title: Novel anti-adhesive barrier Biobarrier reduces growth of colon cancer cells.
abstract: BACKGROUND
  Postoperative peritoneal carcinomatosis together with adhesion formation are considered as two major clinical complications after resection of malignant abdominal tumors, jeopardizing the beneficial effect of the curative surgery. Biobarrier is a novel anti-adhesive barrier fulfilling the criteria for a good adhesion preventive agent, possessing biochemical properties that may enable it to function as a dual efficient device, reducing both adhesion and tumor development. This study aims to evaluate the effect of novel anti-adhesive device Biobarrier on intra-abdominal tumor progression.
  
  
  METHODS
  Cells from cancer cell line BN7005H1D2 were treated with Biobarrier to determine the effect of Biobarrier on cell attachment and viability in vitro. For the in vivo experiments, bilateral peritoneal trauma was inflicted in a reproducible syngeneic rat model. To mimic the clinical situation, the animals received an intraperitoneal injection of BN7005H1D2 cancer cells at the end of surgery, resembling perioperative tumor spill after intraperitoneal instillation of Biobarrier. Animals without given anti-adhesive treatment were used as control.
  
  
  RESULTS
  Biobarrier applied in vitro hindered cells from attachment to the wells. In vivo treatment with Biobarrier significantly reduced tumor growth at both sites of surgical trauma (P = 0.001 and 0.015) and other non-traumatized intraperitoneal sites (P = 0.021).
  
  
  CONCLUSIONS
  Biobarrier maybe effective in reducing intra-abdominal tumor cell implantation with subsequent tumor development in conjunction with peritoneal trauma in a syngeneic rat model.
authors.affiliation: Department of Surgery, Skåne University Hospital at Lund, Sweden; Department of Clinical Sciences, Lund University, Lund, Sweden.
authors.forename: Monika
authors.initials: M
authors.lastname: Posaric-Bauden
authors.affiliation: Department of Surgery, Skåne University Hospital at Lund, Sweden; Department of Clinical Sciences, Lund University, Lund, Sweden.
authors.forename: Karolin
authors.initials: K
authors.lastname: Isaksson
authors.affiliation: Department of Surgery, Skåne University Hospital at Lund, Sweden; Department of Clinical Sciences, Lund University, Lund, Sweden.
authors.forename: Daniel
authors.initials: D
authors.lastname: Akerberg
authors.affiliation: Department of Surgery, Skåne University Hospital at Lund, Sweden; Department of Clinical Sciences, Lund University, Lund, Sweden.
authors.forename: Roland
authors.initials: R
authors.lastname: Andersson
authors.affiliation: Department of Surgery, Skåne University Hospital at Lund, Sweden; Department of Clinical Sciences, Lund University, Lund, Sweden. Electronic address: bobby.tingstedt@med.lu.se.
authors.forename: Bobby
authors.initials: B
authors.lastname: Tingstedt
mesh_terms: D000008:Abdominal Neoplasms / Q000473:pathology / Q000601:surgery; D000230:Adenocarcinoma / Q000473:pathology / Q000601:surgery*; D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D008297:Male; D008567:Membranes, Artificial; D009368:Neoplasm Transplantation; D010534:Peritoneal Neoplasms / Q000473:pathology / Q000601:surgery*; D011108:Polymers; D011914:Rats, Inbred BN; D000267:Tissue Adhesions / Q000517:prevention & control; D047368:Tumor Burden
chemical_list: D008567:Membranes, Artificial; D011108:Polymers
keywords: Anti-adhesive barrier; Biobarrier; Peritoneal carcinomatosis