title: A preliminary study of arecoline and guvacoline presence in the saliva of a "betel-quid" chewer using liquid-chromatography ion trap mass spectrometry.
abstract: It has been reported that the parasympathomimetic alkaloid arecoline and the nootropic agent guvacoline have been detected in areca nut (Areca catechu L.) during extraction using a basic medium. Here, we have studied the detection of arecoline and guvacoline in vivo in saliva of a "betel-quid" chewer using liquid-chromatography ion trap mass spectrometry. In this paper, we provide evidence that guvacoline is absent in the neutral aqueous extract of betel nut, but is present in abundance in the aqueous extract with added time (pH 11.9). In an in vivo experiment, we demonstrated that guvacoline is present in the salivary extracts in the mouth with time (pH 9.5) and without lime (pH5.3).
authors.forename: Adnan A
authors.initials: AA
authors.lastname: Kadi
authors.forename: Mohamed W
authors.initials: MW
authors.lastname: Attwa
authors.forename: A F M Motiur
authors.initials: AF
authors.lastname: Rahman
mesh_terms: D000328:Adult; D001114:Areca; D001115:Arecoline / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008409:Mastication; D009539:Nicotinic Acids / Q000032:analysis / Q000737:chemistry; D012463:Saliva / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D009539:Nicotinic Acids; D001115:Arecoline; C000588631:guvacoline