title: Identification and characterization of suppressor mutants of spl11- mediated cell death in rice.
abstract: Lesion mimic mutants have been used to dissect programmed cell death (PCD) and defense-related pathways in plants. The rice lesion-mimic mutant spl11 exhibits race nonspecific resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae and the fungal pathogen Magnaporthe oryzae. Spl11 encodes an E3 ubiquitin ligase and is a negative regulator of PCD in rice. To study the regulation of Spl11-mediated PCD, we performed a genetic screen and identified three spl11 cell-death suppressor (sds) mutants. These suppressors were characterized for their resistance to X. oryzae pv. oryzae and M. oryzae and for their expression of defense-related genes. The suppression of the cell-death phenotypes was generally correlated with reduced expression of defense-related genes. When rice was challenged with avirulent isolates of M. oryzae, the disease phenotype was unaffected in the sds mutants, indicating that the suppression might be Spl11-mediated pathway specific and may only be involved in basal defense. In addition, we mapped one of the suppressor mutations to a 140-kb interval on the long arm of rice chromosome 1. Identification and characterization of these sds mutants should facilitate our efforts to elucidate the Spl11-mediated PCD pathway.
authors.forename: Gautam S
authors.initials: GS
authors.lastname: Shirsekar
authors.forename: Miguel E
authors.initials: ME
authors.lastname: Vega-Sanchez
authors.forename: Alicia
authors.initials: A
authors.lastname: Bordeos
authors.forename: Marietta
authors.initials: M
authors.lastname: Baraoidan
authors.forename: Andrew
authors.initials: A
authors.lastname: Swisshelm
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Fan
authors.forename: Chan Ho
authors.initials: CH
authors.lastname: Park
authors.forename: Hei
authors.initials: H
authors.lastname: Leung
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Wang
mesh_terms: D016923:Cell Death; D002874:Chromosome Mapping; D018506:Gene Expression Regulation, Plant; D020082:Magnaporthe / Q000472:pathogenicity*; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D010641:Phenotype; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D057865:Plant Immunity; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism; D014974:Xanthomonas / Q000472:pathogenicity*
chemical_list: D010940:Plant Proteins; D044767:Ubiquitin-Protein Ligases