title: [In vitro effect of 5-hydroxytryptamine on motility and length of Schistosoma japonicum primary sporocysts].
abstract: OBJECTIVE
  To study in vitro the effect of 5-hydroxytryptamine (5-HT) on the motility and length of primary sporocysts of Schistosoma japonicum and select the optimal concentration and time of 5-HT.
  
  
  METHODS
  Eggs of S. japonicum were harvested from livers of the infected mice 6-8 weeks after infection, which were then incubated with water. The miracidia were axenically cultured in 1/2 RPMI 1640 containing 10% calf serum and a moderate amount of antibiotics (100 U/ml penicillin G and 100 microg/ml streptomycin) for 48 hours. They became mother sporocysts and were divided into two groups. Parasites in the first group were treated by 5-HT under concentrations of 0, 0.1, 1, 10, 100 and 1000 micromol/L for 48 hours, respectively. Those parasites in the second group were treated by 5-HT of 10 micromol/L for 0.16, 6, 24 and 48 hours, respectively. The motility, length and succinic dehydrogenase (SDH) activity of the parasites were measured under Olympus microscope.
  
  
  RESULTS
  Along with the increase of 5-HT concentration, the motility and length of the mother sporocysts all increased gradually. With the 5-HT concentration of 10 micromol/L, both of them reached a maximum value, being (65.6 +/- 1.5)% and (131.4 +/- 9.2) microm respectively. Meanwhile, along with the prolongation of treatment time, the motility and length also increased gradually. The motility reached a maximal value at 24 hours post-treatment. So did the length and SDH activity of the parasites at 48 hours post-treatment.
  
  
  CONCLUSIONS
  5-HT shows a significant effect on the motility and length of mother sporocysts of S. japonicum and its optimal concentration is 10 micromol/L under in vitro conditions.
authors.forename: Xiang-Ling
authors.initials: XL
authors.lastname: Zhou
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Dong
authors.forename: Ming-Sen
authors.initials: MS
authors.lastname: Jiang
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Huang
mesh_terms: D000818:Animals; D008099:Liver / Q000469:parasitology; D051379:Mice; D008815:Mice, Inbred Strains; D033661:Oocysts / Q000187:drug effects* / Q000502:physiology; D012549:Schistosoma japonicum / Q000033:anatomy & histology / Q000187:drug effects* / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology*
chemical_list: D012701:Serotonin