title: Investigating spermatogenesis in Drosophila melanogaster.
abstract: The process of spermatogenesis in Drosophila melanogaster provides a powerful model system to probe a variety of developmental and cell biological questions, such as the characterization of mechanisms that regulate stem cell behavior, cytokinesis, meiosis, and mitochondrial dynamics. Classical genetic approaches, together with binary expression systems, FRT-mediated recombination, and novel imaging systems to capture single cell behavior, are rapidly expanding our knowledge of the molecular mechanisms regulating all aspects of spermatogenesis. This methods chapter provides a detailed description of the system, a review of key questions that have been addressed or remain unanswered thus far, and an introduction to tools and techniques available to probe each stage of spermatogenesis.
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Rafael S
authors.initials: RS
authors.lastname: Demarco
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, 0379 Montebello, Norway; Centre for Cancer Biomedicine, Faculty of Medicine, Oslo University Hospital, 0379 Montebello, Norway.
authors.forename: Åsmund H
authors.initials: ÅH
authors.lastname: Eikenes
authors.affiliation: Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, 0379 Montebello, Norway; Centre for Cancer Biomedicine, Faculty of Medicine, Oslo University Hospital, 0379 Montebello, Norway.
authors.forename: Kaisa
authors.initials: K
authors.lastname: Haglund
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: leannejones@ucla.edu.
authors.forename: D Leanne
authors.initials: DL
authors.lastname: Jones
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics; D015509:Developmental Biology / Q000379:methods; D004330:Drosophila; D018507:Gene Expression Regulation, Developmental; D008297:Male; D013091:Spermatogenesis / Q000235:genetics*; D014158:Transcription, Genetic
keywords: Cytokinesis; Drosophila; Germ line; Meiosis; Spermatogenesis; Stem cell