title: Recent applications of superresolution microscopy in neurobiology.
abstract: Chemical synapses in brain are structural differentiations where excitatory or inhibitory signals are vectorially transmitted between two neurons. Excitatory synapses occur mostly on dendritic spines, submicron sized protrusions of the neuronal dendritic arborizations. Axons establish contacts with these tiny specializations purported to be the smallest functional processing units in the central nervous system. The minute size of synapses and their macromolecular constituents creates an inherent difficulty for imaging but makes them an ideal object for superresolution microscopy. Here we discuss some representative examples of nanoscopy studies, ranging from quantification of receptors and scaffolding proteins in postsynaptic densities and their dynamic behavior, to imaging of synaptic vesicle proteins and dendritic spines in living neurons or even live animals.
authors.affiliation: Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany; Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Faßberg 11, 37077 Göttingen, Germany. Electronic address: kwillig@gwdg.de.
authors.forename: Katrin I
authors.initials: KI
authors.lastname: Willig
authors.affiliation: Laboratory of Molecular Neurobiology, BIOMED, Faculty of Medical Sciences, Catholic University of Argentina, UCA-CONICET, 1107 Buenos Aires, Argentina. Electronic address: rtfjb1@gmail.com.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Barrantes
mesh_terms: D000818:Animals; D001921:Brain; D008853:Microscopy / Q000379:methods*; D009446:Neurobiology / Q000379:methods*; D011506:Proteins / Q000737:chemistry; D013569:Synapses
chemical_list: D011506:Proteins