title: Lebecin, a new C-type lectin like protein from Macrovipera lebetina venom with anti-tumor activity against the breast cancer cell line MDA-MB231.
abstract: C-type lectins like proteins display various biological activities and are known to affect especially platelet aggregation. Few of them have been reported to have anti-tumor effects. In this study, we have identified and characterized a new C-type lectin like protein, named lebecin. Lebecin is a heterodimeric protein of 30 kDa. The N-terminal amino acid sequences of both subunits were determined by Edman degradation and the entire amino acid sequences were deduced from cDNAs. The precursors of both lebecin subunits contain a 23-amino acid residue signal peptide and the mature α and β subunits are composed of 129 and 131 amino acids, respectively. Lebecin is shown to be a potent inhibitor of MDA-MB231 human breast cancer cells proliferation. Furthermore, lebecin dose-dependently inhibited the integrin-mediated attachment of these cells to different adhesion substrata. This novel C-type lectin also completely blocked MDA-MB231 cells migration towards fibronectin and fibrinogen in haptotaxis assays.
authors.affiliation: Laboratoire des venins et biomolécules thérapeutiques, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis Belvédère, Tunisia. Electronic address: jed.jebali@yahoo.fr.
authors.forename: Jed
authors.initials: J
authors.lastname: Jebali
authors.affiliation: Laboratoire des venins et biomolécules thérapeutiques, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis Belvédère, Tunisia.
authors.forename: Emna
authors.initials: E
authors.lastname: Fakhfekh
authors.affiliation: Laboratoire des venins et biomolécules thérapeutiques, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis Belvédère, Tunisia.
authors.forename: Maram
authors.initials: M
authors.lastname: Morgen
authors.affiliation: Laboratoire des venins et biomolécules thérapeutiques, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis Belvédère, Tunisia.
authors.forename: Najet
authors.initials: N
authors.lastname: Srairi-Abid
authors.affiliation: USCR séquenceur de protéines, Faculté des sciences de Sfax, Route de Soukra, km 3.5, BP 1171, 3000 Sfax, Tunisia.
authors.forename: Hafedh
authors.initials: H
authors.lastname: Majdoub
authors.affiliation: Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les Eucaryotes, Centre de la Biotechnologie de Sfax (CBS), Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Gargouri
authors.affiliation: Laboratoire des venins et biomolécules thérapeutiques, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis Belvédère, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: El Ayeb
authors.affiliation: Aix Marseille Université, Institut National de la Santé et de la Recherche Médicale, UMR_S 911, Marseille, France.
authors.forename: José
authors.initials: J
authors.lastname: Luis
authors.affiliation: Laboratoire des venins et biomolécules thérapeutiques, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis Belvédère, Tunisia.
authors.forename: Naziha
authors.initials: N
authors.lastname: Marrakchi
authors.affiliation: Laboratoire des venins et biomolécules thérapeutiques, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis Belvédère, Tunisia; Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092, Tunisia.
authors.forename: Sameh
authors.initials: S
authors.lastname: Sarray
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry / Q000302:isolation & purification; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014757:Viper Venoms / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017819:Viperidae
chemical_list: D037181:Lectins, C-Type; D014757:Viper Venoms; C519594:lebectin protein, Macrovipera lebetina
keywords: Cell adhesion; Cell migration; Molecular cloning; Molecular modeling; Snake venom