title: Absence of correlation between IL-28B gene polymorphisms and the clinical presentation of chronic hepatitis B in an Amazon Brazilian population.
abstract: OBJECTIVE
  The present study investigated the prevalence of the IL-28B polymorphisms rs12979860 and rs8099917 in chronic hepatitis B patients from a case study in Eastern Amazonia.
  
  
  METHODS
  In total, 65 chronically infected HBV patients and 97 healthy subjects who were anti-HBc and anti-HBs positive (control group) were evaluated between May 2011 and December 2012. The groups of patients were designated as inactive carriers, chronic hepatitis without cirrhosis, and chronic hepatitis with cirrhosis based on clinical, pathological, biochemical, hematological, and virological variables. The patients were genotyped using quantitative real-time PCR.
  
  
  RESULTS
  The frequencies of the rs12979860 polymorphism were similar between the infected group (32.3% CC, 41.5% CT, and 26.2 TT) and the control population (35% CC, 47.4% CT, and 17.6% TT), and the frequencies of the rs8099917 polymorphism (7.7% GG, 35.4% GT, and 56.9% TT versus 7.2% GG, 35.1% GT, and 57.7% TT) were also similar in both groups. The associations between the rs12979860 and rs8099917 polymorphisms and the clinical manifestations were not statistically significant.
  
  
  CONCLUSIONS
  In conclusion, these polymorphisms had a similar distribution between infected and control groups, indicating that they were not associated with susceptibility and the clinical evolution of hepatitis B in the examined population.
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Umarizal, 66050-380 Belém, PA, Brazil ; Holy House of Mercy Foundation of Pará, Umarizal, 66050-380 Belém, PA, Brazil.
authors.forename: Simone Regina Souza da Silva
authors.initials: SR
authors.lastname: Conde
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Umarizal, 66050-380 Belém, PA, Brazil.
authors.forename: Luciana L
authors.initials: LL
authors.lastname: Rocha
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Umarizal, 66050-380 Belém, PA, Brazil.
authors.forename: Vanessa M
authors.initials: VM
authors.lastname: Ferreira
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Umarizal, 66050-380 Belém, PA, Brazil.
authors.forename: Julius Caesar Mendes Soares
authors.initials: JC
authors.lastname: Monteiro
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Umarizal, 66050-380 Belém, PA, Brazil.
authors.forename: Nathália Karla Fonseca
authors.initials: NK
authors.lastname: Filgueiras
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Umarizal, 66050-380 Belém, PA, Brazil.
authors.forename: Pedro Alves de Almeida
authors.initials: PA
authors.lastname: Lins
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Umarizal, 66050-380 Belém, PA, Brazil.
authors.forename: Bruna Tereza Silva
authors.initials: BT
authors.lastname: dos Santos
authors.affiliation: Virology Laboratory, Institute of Biological Sciences, Federal University of Pará, Guamá, 66075-110 Belém, PA, Brazil.
authors.forename: Felipe Bonfim
authors.initials: FB
authors.lastname: Freitas
authors.affiliation: Virology Laboratory, Institute of Biological Sciences, Federal University of Pará, Guamá, 66075-110 Belém, PA, Brazil.
authors.forename: Ednelza da Silva
authors.initials: Eda S
authors.lastname: Graça
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Umarizal, 66050-380 Belém, PA, Brazil.
authors.forename: Sâmia
authors.initials: S
authors.lastname: Demachki
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Umarizal, 66050-380 Belém, PA, Brazil.
authors.forename: Marialva Tereza Ferreira
authors.initials: MT
authors.lastname: de Araújo
authors.affiliation: Virology Laboratory, Institute of Biological Sciences, Federal University of Pará, Guamá, 66075-110 Belém, PA, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Ishak
authors.affiliation: Virology Laboratory, Institute of Biological Sciences, Federal University of Pará, Guamá, 66075-110 Belém, PA, Brazil.
authors.forename: Antonio C R
authors.initials: AC
authors.lastname: Vallinoto
mesh_terms: D001938:Brazil; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D019694:Hepatitis B, Chronic / Q000235:genetics*; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D008099:Liver / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda