title: Vitamin C supplementation does not influence plasma and blood mononuclear cell IL-6 and IL-10 levels after exercise.
abstract: The aim of this study was to determine whether the highest vitamin C supplementation associated with complete bioavailability influences the plasma and blood mononuclear cell IL-6 and IL-10 response to exercise. A double-blinded study of supplementation with vitamin C was performed. After 15 days of supplementation with vitamin C (500 mg · day(-1), n = 16) or a placebo (n = 15), participants in the study completed a 15-km run competition. Blood samples were taken before and after competition. Oxidative stress markers, antioxidants, cortisol, IL-6 and IL-10 were determined in plasma or serum. IL-6 and IL-10 protein and mRNA levels were measured in blood mononuclear cells. Although higher plasma and blood mononuclear cell vitamin C levels were observed in the supplemented group when compared with the placebo one, the two groups showed identical exercise-induced changes in all the measured parameters. Exercise induced increased IL-6 and IL-10 levels in plasma and blood mononuclear cells. IL-6 and IL-10 mRNA levels in blood mononuclear cells increased after the competition. After recovery, IL-6 mRNA returned to basal levels and IL-10 mRNA levels remained elevated. In conclusion, exercise induced increased IL-6 and IL-10 production in blood mononuclear cells. However, vitamin C supplementation did not influence IL-6 and IL-10 response to exercise.
authors.affiliation: a Research Group on Evidence , Lifestyles & Health, Department of Nursing and Physiotherapy, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands , Palma de Mallorca , Spain.
authors.forename: Antoni
authors.initials: A
authors.lastname: Aguiló
authors.forename: Marta
authors.initials: M
authors.lastname: Monjo
authors.forename: Carlos
authors.initials: C
authors.lastname: Moreno
authors.forename: Pau
authors.initials: P
authors.lastname: Martinez
authors.forename: Sonia
authors.initials: S
authors.lastname: Martínez
authors.forename: Pedro
authors.initials: P
authors.lastname: Tauler
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000097:blood / Q000494:pharmacology*; D019587:Dietary Supplements; D004311:Double-Blind Method; D015444:Exercise / Q000502:physiology; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007249:Inflammation / Q000097:blood; D016753:Interleukin-10 / Q000097:blood* / Q000235:genetics; D015850:Interleukin-6 / Q000097:blood* / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism; D012420:Running / Q000502:physiology*; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D015850:Interleukin-6; D012333:RNA, Messenger; D014815:Vitamins; D016753:Interleukin-10; D001205:Ascorbic Acid; D006854:Hydrocortisone
keywords: IL-10; IL-6; antioxidant supplementation; exercise; inflammation; oxidative stress