title: Characterization of the first report of Mycobacterium timonense infecting an HIV patient in an Ecuadorian hospital.
abstract: Mycobacterium timonense is a non-tuberculous mycobacteria (NTM) described in southern France in 2009, and to our knowledge, not reported again as a human pathogen in indexed literature. The aim of this work was to characterize the first clinical isolate of M. timonense in Ecuador. Time of growth, biochemical tests, thin layer growth test, PCR-RFLP analysis of the hsp65 gene and MALDI-TOF spectra analysis were not able to identify the species. The species identification was achieved through sequencing of rrs, hsp65 and rpoB genes. The results highlight the necessity to set up a sequencing method to identify emerging NTM in Ecuadorian clinical facilities.
authors.affiliation: Servicio de Microbiología y Tuberculosis, Hospital Vozandes, Quito, Ecuador; Facultad de Medicina, Pontificia Universidad Católica del Ecuador, Quito, Ecuador; Unidad de Investigaciones en Biomedicina, Zurita & Zurita Laboratorios, Quito, Ecuador.
authors.forename: J
authors.initials: J
authors.lastname: Zurita
authors.forename: D
authors.initials: D
authors.lastname: Ortega-Paredes
authors.forename: M
authors.initials: M
authors.lastname: Mora
authors.forename: N
authors.initials: N
authors.lastname: Espinel
authors.forename: H
authors.initials: H
authors.lastname: Parra
authors.forename: L
authors.initials: L
authors.lastname: Febres
authors.forename: C
authors.initials: C
authors.lastname: Zurita-Salinas
mesh_terms: D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D018834:Chaperonin 60 / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004484:Ecuador; D005602:France; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis* / Q000382:microbiology*; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004275:DNA, Ribosomal; C074119:heat-shock protein 65, Mycobacterium; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit
keywords: Ecuador; Mycobacterium avium complex; Mycobacterium timonense; Non-tuberculous mycobacteria