title: Preparation of ferrocene-functionalized gold nanoparticles by primer extension reaction on the particle surface.
abstract: DNA molecules possessing multiple ferrocene (Fc) molecules as a redox active probe were prepared by the primer extension (PEX) reaction using a 2'-deoxyuridine-5'-triphosphate derivative in which Fc was connected to the C5-position of the uridine by a diethylene glycol linker. Gold nanoparticles (AuNP) covered with DNA possessing the Fc molecules were prepared by the PEX reaction on the surface. The AuNP-FcDNA conjugates exhibit a detectable electrochemical signal due to the Fc molecules. Possible application of the PEX reaction on AuNP is demonstrated for the detection of a single nucleotide mutation in the target DNA.
authors.affiliation: Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan. Electronic address: takada@eng.u-hyogo.ac.jp.
authors.forename: Tadao
authors.initials: T
authors.lastname: Takada
authors.affiliation: Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Tochi
authors.affiliation: Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Nakamura
authors.affiliation: Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Yamana
mesh_terms: D001483:Base Sequence; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D000075163:Metallocenes; D008969:Molecular Sequence Data; D010316:Particle Size; D013499:Surface Properties
chemical_list: D005296:Ferrous Compounds; D061925:Immobilized Nucleic Acids; D000075163:Metallocenes; D006046:Gold; C004998:ferrocene
keywords: DNA; Electrochemistry; Ferrocene; Gold nanoparticles; Polymerase extension reaction