title: The role of podoplanin in the biology of differentiated thyroid cancers.
abstract: Podoplanin (PDPN), a mucin-type transmembrane glycoprotein specific to the lymphatic system is expressed in a variety of human cancers, and is regarded as a factor promoting tumor progression. The purpose of this study was to elucidate the molecular role of PDPN in the biology of thyroid cancer cells. PDPN expression was evaluated in primary thyroid carcinomas and thyroid carcinoma cell lines by RT-qPCR, Western blotting, IF and IHC. To examine the role of podoplanin in determining a cell's malignant potential (cellular migration, invasion, proliferation, adhesion, motility, apoptosis), a thyroid cancer cell line with silenced PDPN expression was used. We observed that PDPN was solely expressed in the cancer cells of 40% of papillary thyroid carcinoma (PTC) tissues. Moreover, PDPN mRNA and protein were highly expressed in PTC-derived TPC1 and BcPAP cell lines but were not detected in follicular thyroid cancer derived cell lines. PDPN knock-down significantly decreased cellular invasion, and modestly reduced cell migration, while proliferation and adhesion were not affected. Our results demonstrate that PDPN mediates the invasive properties of cells derived from papillary thyroid carcinomas, suggesting that podoplanin might promote PTC progression.
authors.affiliation: Department of Biochemistry and Molecular Biology, Center of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rudzińska
authors.affiliation: Department of Biochemistry and Molecular Biology, Center of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Damian
authors.initials: D
authors.lastname: Gaweł
authors.affiliation: Department of Biochemistry and Molecular Biology, Center of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Sikorska
authors.affiliation: Department of Biochemistry and Molecular Biology, Center of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Kamila M
authors.initials: KM
authors.lastname: Karpińska
authors.affiliation: Department of Pathology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland.
authors.forename: Mirosław
authors.initials: M
authors.lastname: Kiedrowski
authors.affiliation: Department of General and Endocrinological Surgery, Copernicus Memorial Hospital, Łódź, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Stępień
authors.affiliation: Department of General and Endocrinological Surgery, Copernicus Memorial Hospital, Łódź, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Marchlewska
authors.affiliation: Department of Biochemistry and Molecular Biology, Center of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Czarnocka
mesh_terms: D063646:Carcinogenesis / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009361:Neoplasm Invasiveness / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured; D000098853:Podoplanin
chemical_list: D008562:Membrane Glycoproteins; D012333:RNA, Messenger; C117468:PDPN protein, human; D000098853:Podoplanin