title: Differential expression of microRNA-320a, -145, and -192 along the continuum of normal mucosa to high-grade dysplastic adenomas of the colorectum.
abstract: BACKGROUND
  MicroRNA (miR)-320a, miR-145, and miR-192 have been shown to play a role in colorectal carcinogenesis and metastasis. We examined if there is a difference in expression during the histologic progression from normal mucosa (NM) to high-grade dysplastic adenomas (HG).
  
  
  METHODS
  Genome-wide miRNA expression profiling was performed on 113 colon adenomas. Information included histologic type, tumor grade, location, sex, age, family, and smoking history. A 2-way ANOVA was performed to evaluate the effect of the following factors adjusted for scan dates: location, sex, age, family history, smoking, and histology.
  
  
  RESULTS
  The expression of miR-320a increased; miR-145 and miR-192 expression decreased (P < .0001), with higher histologic grade, and were independent of age, sex, family history, and smoking status.
  
  
  CONCLUSIONS
  The miRs studied had statistically significant changes in expression with progression of histologic grade. These changes may signify progression of normal mucosa to HG and potentially serve as early markers for disease progression and differentiating high- from low-risk adenomas.
authors.affiliation: Division of Colorectal Surgery, Department of Surgery, Oregon Health and Science University, 3181 S.W. Sam Jackson Park Road, L223A, Portland, OR 97239, USA. Electronic address: tsikitis@ohsu.edu.
authors.forename: Vassiliki L
authors.initials: VL
authors.lastname: Tsikitis
authors.affiliation: Division of Colorectal Surgery, Department of Surgery, Oregon Health and Science University, 3181 S.W. Sam Jackson Park Road, L223A, Portland, OR 97239, USA.
authors.forename: Ian
authors.initials: I
authors.lastname: White
authors.affiliation: Knight Cancer Institute, Oregon Health and Science University, Portland, OR, USA; Integrated Genomics Laboratory, Oregon Health and Science University, Portland, OR, USA.
authors.forename: Motomi
authors.initials: M
authors.lastname: Mori
authors.affiliation: Integrated Genomics Laboratory, Oregon Health and Science University, Portland, OR, USA.
authors.forename: Amiee
authors.initials: A
authors.lastname: Potter
authors.affiliation: Arizona Cancer Center, Tucson, AZ, USA.
authors.forename: Achyut
authors.initials: A
authors.lastname: Bhattcharyya
authors.affiliation: Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Stanley R
authors.initials: SR
authors.lastname: Hamilton
authors.affiliation: Arizona Cancer Center, Tucson, AZ, USA.
authors.forename: Julie
authors.initials: J
authors.lastname: Buckmeier
authors.affiliation: Arizona Cancer Center, Tucson, AZ, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Lance
authors.affiliation: Arizona Cancer Center, Tucson, AZ, USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Thompson
mesh_terms: D018256:Adenomatous Polyps / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C541010:MIRN145 microRNA, human; C546783:MIRN192 microRNA, human; C572916:MIRN320 microRNA, human; D035683:MicroRNAs
keywords: Adenocarcinomas; Colorectal carcinogenesis and metastasis; High-grade dysplastic adenomas; MicroRNAs; miR expression