title: Persistence of α-cypermethrin residues in milk of lactating donkeys (Equus asinus) using UHPLC-MS/MS.
abstract: The aim of this study was to measure the persistence of residues of the pyrethroid insecticide α-cypermethrin (ACYP) in the milk of lactating donkeys following pour-on treatment. Milk was collected from animals (n = 7) before the treatment and at 12, 24, 36, 48, 60, 72 and 84 h post-treatment. The last sampling was taken 7 days post-treatment (168 h). Milk samples were analysed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). The analytical method was validated following requirements of Commission Decision 2002/657/EC. All samples showed levels of ACYP below the maximum residue limit (MRL) of 20 μg kg(-1) established for bovine milk (Commission Regulation (EU) No. 37/2010). The results demonstrate that there is minimal partitioning of ACYP into milk in lactating donkeys from pour-on treatment.
authors.affiliation: a Department of Veterinary Medicine and Animal Production , University of Naples 'Federico II' , Naples , Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Chirollo
authors.forename: Anita
authors.initials: A
authors.lastname: Radovnikovic
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Veneziano
authors.forename: Raffaele
authors.initials: R
authors.lastname: Marrone
authors.forename: Tiziana
authors.initials: T
authors.lastname: Pepe
authors.forename: Martin
authors.initials: M
authors.lastname: Danaher
authors.forename: Aniello
authors.initials: A
authors.lastname: Anastasio
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004478:Ectoparasitic Infestations / Q000188:drug therapy / Q000378:metabolism / Q000662:veterinary; D018397:Equidae / Q000378:metabolism* / Q000469:parasitology; D005260:Female; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D007223:Infant; D007774:Lactation; D008452:Maximum Allowable Concentration; D008892:Milk / Q000737:chemistry* / Q000633:toxicity; D010573:Pesticide Residues / Q000032:analysis* / Q000633:toxicity; D011722:Pyrethrins / Q000008:administration & dosage / Q000032:analysis* / Q000633:toxicity; D053719:Tandem Mass Spectrometry; D019155:Veterinary Drugs / Q000008:administration & dosage / Q000032:analysis* / Q000633:toxicity
chemical_list: D010573:Pesticide Residues; D011722:Pyrethrins; D019155:Veterinary Drugs; C017160:cypermethrin
keywords: HPLC-MS/MS; alpha-cypermethrin; depletion; donkeys; milk; residues