title: Confirmation of isolates of Listeria by conventional and real-time PCR.
abstract: Polymerase chain reaction (PCR) is an invaluable diagnostic technique in microbiology for rapid and specific detection and confirmation of microbial isolates from food and the environment. PCR is a simple, sensitive, specific, and reproducible assay and can be performed in conventional or in real-time formats. Here, we describe the application of real-time and conventional PCR-based methods for confirmation of presumptive Listeria isolates.
authors.affiliation: Instituto Tecnológico Agrario (ITACyL), Junta de Castilla y León, Ctra. Burgos, km119, 47071, Valladolid, Spain, rodlazda@gmail.com.
authors.forename: David
authors.initials: D
authors.lastname: Rodríguez-Lázaro
authors.forename: Marta
authors.initials: M
authors.lastname: Hernández
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004783:Environmental Microbiology; D008087:Listeria / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods
chemical_list: D004269:DNA, Bacterial