title: Antiplasmodial activity of certain medicinal plants against chloroquine resistant Plasmodium berghei infected white albino BALB/c mice.
abstract: In the present study of antimalarial efficacy, aqueous extracts of leaves and unripe fruits of Psidium guajava, leaves of Ocimum sanctum and leaves of Murraya koenigii are evaluated against Plasmodium berghei (chloroquine resistant NK65 strain) infected white albino BALB/c mice. A 7 days oral administration was adopted with different dosage viz., 350 mg, 750 mg and 1,000 mg/kg body weight as treatment schedule along with parasite (Group I) and drug control with Chloroquine, 50 mg/kg body weight (Group II). All the parts were extracted based on the decoction method, which is commonly seen among the villagers/tribes as their usual method of preparation of decoction for most of the ailments. The antimalarial activities were evaluated from the giemsa stained blood smears collected from different treated groups of mice used in this experiment. The antiplasmodial effect that is percent parasitaemia and percent suppression (values in parenthesis) showed by the treated groups of mice at 350 mg/kg b. wt. by the aqueous extracts of P. guajava leaves (Group III) was 19.8 ± 1.22 (73.7 %), P. guajava unripe fruits (Group IV) was 52.7 ± 2.19 (30.0 %), leaves of O. sanctum (Group V) was 64.0 ± 0.73 (15.1 %) and leaves of M. koenigii (Group VI) was 28.9 ± 0.81 (61.6 %) whereas at 750 mg/kg b. wt., it all showed 10.3 ± 0.7 (80.2 %), 26.3 ± 0.52 (65.1 %), 42.0 ± 0.47 (44.2 %) and 14.9 ± 0.46 (71.5 %) whereas at 1,000 mg/kg b. wt. dose, it all showed 9.2 ± 0.39 (85.8 %), 25.6 ± 0.40 (62.0 %), 41.8 ± 0.29 (35.5 %) and 14.0 ± 0.42 (76.9 %) respectively.
authors.affiliation: Medical Entomology Division, Defence Research Laboratory, Defence Research & Development Organisation, Post Bag No: 2, Tezpur, 784 001 India.
authors.forename: C
authors.initials: C
authors.lastname: Rajendran
authors.affiliation: CEPTAM, Defence Research & Development Organisation, Timarpur, 110054 New Delhi India.
authors.forename: M
authors.initials: M
authors.lastname: Begam
authors.affiliation: Medical Entomology Division, Defence Research Laboratory, Defence Research & Development Organisation, Post Bag No: 2, Tezpur, 784 001 India.
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Medical Entomology Division, Defence Research Laboratory, Defence Research & Development Organisation, Post Bag No: 2, Tezpur, 784 001 India.
authors.forename: Indra
authors.initials: I
authors.lastname: Baruah
authors.affiliation: Biotechnology Division, Defence Research Laboratory, Defence Research & Development Organisation, Post Bag No: 2, Tezpur, 784 001 India.
authors.forename: H K
authors.initials: HK
authors.lastname: Gogoi
authors.affiliation: Defence Institute of High Altitude Research, Defence Research & Development Organisation, Leh, 194101 Jammu and Kashmir India.
authors.forename: R B
authors.initials: RB
authors.lastname: Srivastava
authors.affiliation: Medical Entomology Division, Defence Research Laboratory, Defence Research & Development Organisation, Post Bag No: 2, Tezpur, 784 001 India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Veer
keywords: In vivo antimalarial bioassay; Medicinal plants; Mice; P. berghei