title: Comparative studies on the interactions of dihydroartemisinin and artemisinin with bovine serum albumin using spectroscopic methods.
abstract: The interactions of dihydroartemisinin (DHA) and artemisinin (ART) with bovine serum albumin (BSA) have been investigated using fluorescence, UV/vis absorption and Fourier transform infrared (FTIR) spectra under simulated physiological conditions. The binding characteristics of DHA/ART and BSA were determined by fluorescence emission and resonance light scattering (RLS) spectra. The quenching mechanism between BSA and DHA/ART is static. The binding constants and binding sites of DHA/ART-BSA systems were calculated at different temperatures (293, 298, 304 and 310 K). According to Förster non-radiative energy transfer theory, the binding distance of BSA to DHA/ART was calculated to be 1.54/1.65 nm. The effect of DHA/ART on the secondary structure of BSA was analyzed using UV/vis absorption, FTIR, synchronous fluorescence and 3D fluorescence spectra. In addition, the effects of common ions on the binding constants of BSA-DHA and BSA-ART systems were also discussed.
authors.affiliation: Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong, 637002, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Cheng
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Jiang
mesh_terms: D037621:Artemisinins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D004735:Energy Transfer; D005453:Fluorescence; D021621:Imaging, Three-Dimensional; D008027:Light; D011487:Protein Conformation; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013816:Thermodynamics
chemical_list: D037621:Artemisinins; D012710:Serum Albumin, Bovine; C039060:artenimol; C031327:artemisinin
keywords: artemisinin; binding constants; bovine serum albumin; dihydroartemisinin; fluorescence spectroscopy