title: Arabidopsis Transporter MGT6 Mediates Magnesium Uptake and Is Required for Growth under Magnesium Limitation.
abstract: Although magnesium (Mg2+) is the most abundant divalent cation in plant cells, little is known about the mechanism of Mg2+uptake by plant roots. Here, we report a key function of Magnesium Transport6 (MGT6)/Mitochondrial RNA Splicing2-4 in Mg2+uptake and low-Mg2+tolerance in Arabidopsis thaliana. MGT6 is expressed mainly in plant aerial tissues when Mg2+levels are high in the soil or growth medium. Its expression is highly induced in the roots during Mg2+deficiency, suggesting a role for MGT6 in response to the low-Mg2+status in roots. Silencing of MGT6 in transgenic plants by RNA interference (RNAi) resulted in growth retardation under the low-Mg2+condition, and the phenotype was restored to normal growth after RNAi plants were transferred to Mg2+-sufficient medium. RNAi plants contained lower levels of Mg2+compared with wild-type plants under low Mg2+but not under Mg2+-sufficient conditions. Further analysis indicated that MGT6 was localized in the plasma membrane and played a key role in Mg2+uptake by roots under Mg2+limitation. We conclude that MGT6 mediates Mg2+uptake in roots and is required for plant adaptation to a low-Mg2+environment.
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha 410081, China Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, State Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Mao
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors.forename: Lianfu
authors.initials: L
authors.lastname: Tian
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, State Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, California 94720.
authors.forename: Renjie
authors.initials: R
authors.lastname: Tang
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Lu
authors.affiliation: Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, State Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, MOF Key Laboratory of Forest Genetics and Biotechnology, Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Jisen
authors.initials: J
authors.lastname: Shi
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors.forename: Liangbi
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors.forename: Dongping
authors.initials: D
authors.lastname: Li
authors.affiliation: Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, State Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, China Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 sluan@berkeley.edu.
authors.forename: Sheng
authors.initials: S
authors.lastname: Luan