title: Temporal proteomic analysis reveals defects in small-intestinal development of porcine fetuses with intrauterine growth restriction.
abstract: The fetus/neonate with intrauterine growth restriction (IUGR) has a high perinatal mortality and morbidity rate, as well as reduced efficiency for nutrients utilization. Our previous studies showed alterations of intestinal proteome in IUGR piglets both at birth and during the nursing period. Considering the potential long-term impacts of fetal programming and substantial increases in amounts of amniotic fluid nutrients from mid-gestation in pigs, the present study involved IUGR porcine fetuses from days 60 to 110 of gestation (mid to late gestation). We identified 59 differentially expressed proteins in the fetal small intestine that are related to intestinal growth, development and reprogramming. Our results further indicated increased abundances of proteins and enzymes associated with oxidative stress, apoptosis and protein degradation, as well as decreased abundances of proteins that are required for maintenance of cell structure and motility, absorption and transport of nutrients, energy metabolism, and protein synthesis in the fetal gut. Moreover, IUGR from middle to late gestation was associated with reduced expression of intestinal proteins that participate in regulation of gene expression and signal transduction. Collectively, these findings provide the first evidence for altered proteomes in the small intestine of IUGR fetuses, thereby predisposing the gut to metabolic defects during gestation and neonatal periods.
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China.
authors.forename: Xiaoqiu
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Feng
authors.affiliation: Department of Animal Science, University of California, Davis, CA 95616, USA.
authors.forename: Huaijun
authors.initials: H
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China.
authors.forename: Taiji
authors.initials: T
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China.
authors.forename: Defa
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China; Department of Animal Science, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China. Electronic address: jkywjj@hotmail.com.
authors.forename: Junjun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism*; D005865:Gestational Age; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000196:embryology* / Q000378:metabolism*; D011247:Pregnancy; D020543:Proteome / Q000378:metabolism*; D013552:Swine
chemical_list: D020543:Proteome
keywords: Development; Fetus; Intestine; Intrauterine growth restriction; Pig