title: Analysis of mRNA translation states in Arabidopsis over the diurnal cycle by polysome microarray.
abstract: Gene regulation at the level of translation occurs in response to environmental perturbation and is increasingly recognized as a factor affecting plant development. Despite extensive knowledge of transcriptional control, very little is known about translational regulation of genes in response to the daily light/dark cycles. Here we describe the experimental layout designed to address how the translation states of genes change at various times during a diurnal cycle in Arabidopsis thaliana seedlings. We have adopted a strategy combining sucrose-gradient profiling of ribosomes and high-throughput microarray analysis of the ribosome-associated mRNA to investigate the translational landscape of the Arabidopsis genome. This is a powerful technique that can be easily extended to study translation regulation in different genetic backgrounds and under various environmental conditions.
authors.affiliation: Department of Biochemistry, Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN, 37996-0840, USA, amissra@utk.edu.
authors.forename: Anamika
authors.initials: A
authors.lastname: Missra
authors.forename: Albrecht G
authors.initials: AG
authors.lastname: von Arnim
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D057906:Circadian Clocks / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger