title: Induction of reparative dentin formation on exposed dental pulp by dentin phosphophoryn/collagen composite.
abstract: The ultimate goal of vital pulp therapy is to regenerate rapidly dentin possessing an excellent quality using a biocompatible, bioactive agent. Dentin phosphophoryn (DPP), the most abundant noncollagenous polyanionic protein in dentin, cross-linked to atelocollagen fibrils was applied to direct pulp capping in rats. After 1, 2, and 3 weeks, the teeth applied were examined on the induction of reparative dentin formation and the response of pulp tissue, compared to calcium hydroxide-based agent conventionally used. The reparative dentin formation induced by DPP/collagen composite was more rapid than by calcium hydroxide. In the morphometrical analysis, the formation rate of reparative dentin by DPP/collagen composite was approximately the same as that by calcium hydroxide at 3 weeks. Nevertheless, the compactness of reparative dentin formed by DPP/collagen composite was much superior to what resulted from calcium hydroxide. Also, DPP/collagen composite showed high covering ability of exposed pulp. Moreover, DPP/collagen composite led only to slight pulp inflammation at the beginning whereas calcium hydroxide formed necrotic layer adjacent to the material and induced severe inflammation in pulp tissue at 1 week. The present study demonstrates a potential for DPP/collagen composite as a rapid biocompatible inducer for the formation of reparative dentin of excellent quality in rats.
authors.affiliation: Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Tobetsu, Hokkaido 061-0293, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Koike
authors.affiliation: Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Tobetsu, Hokkaido 061-0293, Japan.
authors.forename: Mohammad Ali Akbor
authors.initials: MA
authors.lastname: Polan
authors.affiliation: Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Tobetsu, Hokkaido 061-0293, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Izumikawa
authors.affiliation: Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Tobetsu, Hokkaido 061-0293, Japan.
authors.forename: Takashi
authors.identifier: 0000-0001-8816-7187
authors.initials: T
authors.lastname: Saito
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D003782:Dental Pulp / Q000254:growth & development* / Q000378:metabolism; D003804:Dentin / Q000254:growth & development*; D003809:Dentin, Secondary / Q000254:growth & development* / Q000378:metabolism; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D020894:Microfibrils / Q000378:metabolism*; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism; D051381:Rats
chemical_list: D008689:Methacrylates; D010750:Phosphoproteins; C059821:atelocollagen; C017630:phosphophoryn; D003094:Collagen