title: Decellularization and recellularization technologies in tissue engineering.
abstract: Decellularization is the process by which cells are discharged from tissues/organs, but all of the essential cues for cell preservation and homeostasis are retained in a three-dimensional structure of the organ and its extracellular matrix components. During tissue decellularization, maintenance of the native ultrastructure and composition of the extracellular matrix (ECM) is extremely acceptable. For recellularization, the scaffold/matrix is seeded with cells, the final goal being to form a practical organ. In this review, we focus on the biological properties of the ECM that remains when a variety of decellularization methods are used, comparing recellularization technologies, including bioreactor expansion for perfusion-based bioartificial organs, and we discuss cell sources. In the future, decellularization-recellularization procedures may solve the problem of organ assembly on demand.
authors.affiliation: Graduate Institute of Immunology, China Medical University, Taichung, Taiwan.
authors.forename: Ru-Huei
authors.initials: RH
authors.lastname: Fu
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Wang
authors.forename: Shih-Ping
authors.initials: SP
authors.lastname: Liu
authors.forename: Ton-Ru
authors.initials: TR
authors.lastname: Shih
authors.forename: Hsin-Lien
authors.initials: HL
authors.lastname: Lin
authors.forename: Yue-Mi
authors.initials: YM
authors.lastname: Chen
authors.forename: Jiun-Huei
authors.initials: JH
authors.lastname: Sung
authors.forename: Chia-Hui
authors.initials: CH
authors.lastname: Lu
authors.forename: Jing-Rong
authors.initials: JR
authors.lastname: Wei
authors.forename: Zih-Wan
authors.initials: ZW
authors.lastname: Wang
authors.forename: Shyh-Jer
authors.initials: SJ
authors.lastname: Huang
authors.forename: Chang-Hai
authors.initials: CH
authors.lastname: Tsai
authors.forename: Woei-Cherng
authors.initials: WC
authors.lastname: Shyu
authors.forename: Shinn-Zong
authors.initials: SZ
authors.lastname: Lin
mesh_terms: D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism* / Q000648:ultrastructure; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D012997:Solvents / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D004798:Enzymes; D006025:Glycosaminoglycans; D036341:Intercellular Signaling Peptides and Proteins; D012997:Solvents; D003094:Collagen