title: Aligned collagen-GAG matrix as a 3D substrate for Schwann cell migration and dendrimer-based gene delivery.
abstract: The development of artificial off-the-shelf conduits that facilitate effective nerve regeneration and recovery after repair of traumatic nerve injury gaps is of fundamental importance. Collagen-glycosaminoglycan (GAG) matrix mimicking Schwann cell (SC) basal lamina has been proposed as a suitable and biologically rational substrate for nerve regeneration. In the present study, we have focused on the permissiveness of this matrix type for SC migration and repopulation, as these events play an essential role in nerve remodeling. We have also demonstrated that SCs cultured within collagen-GAG matrix are compatible with non-viral dendrimer-based gene delivery, that may allow conditioning of matrix-embedded cells for future gene therapy applications.
authors.affiliation: Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, HMRB 109-3330 Hospital Drive NW, Calgary, AB, T2N4N1, Canada, shakhbazau@gmail.com.
authors.forename: Antos
authors.initials: A
authors.lastname: Shakhbazau
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Archibald
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
authors.forename: Rajiv
authors.initials: R
authors.lastname: Midha
mesh_terms: D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D018920:Coculture Techniques; D003094:Collagen / Q000378:metabolism*; D050091:Dendrimers; D005822:Genetic Vectors; D006025:Glycosaminoglycans / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D012583:Schwann Cells / Q000166:cytology*; D054457:Tissue Scaffolds
chemical_list: D050091:Dendrimers; D006025:Glycosaminoglycans; D003094:Collagen