title: 67 laminin receptor promotes the malignant potential of tumour cells up-regulating lysyl oxidase-like 2 expression in cholangiocarcinoma.
abstract: BACKGROUND
  67 laminin receptor (67LR) plays an important role in the invasion and metastasis of cholangiocarcinoma, but its mechanism remains unclear.
  
  
  OBJECTIVE
  We investigated the clinical significance of 67LR and its relation to lysyl oxidase-like 2 (LOXL2) in 67LR-mediated invasion and metastasis in cholangiocarcinoma.
  
  
  METHODS
  The clinical significance of 67LR and LOXL2 expression and the prognosis of patients were investigated in 73 cancerous and 32 paracancerous tissues by immunohistochemistry. The impact of LOXL2 on invasion, metastasis and 67LR expression was evaluated in cholangiocarcinoma cells by shRNA or expressed-plasmid transfection.
  
  
  RESULTS
  Expression of 67LR was recognized in 35.62% cholangiocarcinoma tissue, and none in paracancerous tissues. LOXL2 was positively correlated with expression of 67LR. Expression of 67LR or LOXL2 in cholangiocarcinomas was significantly associated with lymph node metastasis, differentiation and poor overall survival. Cox analysis showed that 67LR can act as an independent prognostic biomarker of prognosis in cholangiocarcinoma patients. Expression of LOXL2 decreased by knockdown of 67LR and increased by overexpression of 67LR in cholangiocarcinoma cells. Knockdown of LOXL2 reduced invasion and metastasis in vitro and in vivo.
  
  
  CONCLUSIONS
  67LR may regulate the expression of LOXL2 to promote invasion and metastasis in cholangiocarcinoma cells. It could be used as an independent prognostic marker in cholangiocarcinoma patients.
authors.affiliation: Hepatobiliary Surgery Institute, Laboratory of Liver Transplantation, Southwest Hospital, Third Military Medical University, Chongqing, PR China; Hepatobiliary Surgery Department, First People Hospital of Yunnan Province, Kunming University of Science and Technology, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Hepatobiliary Surgery Institute, Laboratory of Liver Transplantation, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Dajing
authors.initials: D
authors.lastname: Li
authors.affiliation: Hepatobiliary Surgery Institute, Laboratory of Liver Transplantation, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Li
authors.affiliation: Hepatobiliary Surgery Institute, Laboratory of Liver Transplantation, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Zipei
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Hepatobiliary Surgery Institute, Laboratory of Liver Transplantation, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Li
authors.affiliation: Hepatobiliary Surgery Institute, Laboratory of Liver Transplantation, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Hepatobiliary Surgery Institute, Laboratory of Liver Transplantation, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.affiliation: Hepatobiliary Surgery Institute, Laboratory of Liver Transplantation, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Tian
authors.affiliation: Hepatobiliary Surgery Institute, Laboratory of Liver Transplantation, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Hepatobiliary Surgery Institute, Laboratory of Liver Transplantation, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Dechun
authors.initials: D
authors.lastname: Wang
authors.affiliation: Hepatobiliary Surgery Institute, Laboratory of Liver Transplantation, Southwest Hospital, Third Military Medical University, Chongqing, PR China. Electronic address: 123163137@qq.com.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Wang
mesh_terms: D000368:Aged; D000594:Amino Acid Oxidoreductases / Q000032:analysis* / Q000235:genetics; D000818:Animals; D001650:Bile Duct Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D001653:Bile Ducts, Intrahepatic; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement; D018281:Cholangiocarcinoma / Q000737:chemistry* / Q000235:genetics / Q000556:secondary; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011379:Prognosis; D017474:Receptors, Laminin / Q000032:analysis* / Q000235:genetics; D012269:Ribosomal Proteins / Q000032:analysis* / Q000235:genetics; D015996:Survival Rate; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C510624:RPSA protein, human; D017474:Receptors, Laminin; D012269:Ribosomal Proteins; D000594:Amino Acid Oxidoreductases; C119091:LOXL2 protein, human
keywords: 67kDa laminin receptor; Cholangiocarcinoma; Lysyl oxidase-like 2; Prognosis