title: 'Immunosequencing' of heparan sulfate from human cell lines and rat kidney: the (GlcNS6S-IdoA2S)₃ motif, recognized by antibody NS4F5, is located towards the non-reducing end.
abstract: HS (heparan sulfate) is a long linear polysaccharide, variably modified by epimerization and sulfation reactions, and is organized into different domains defined by the extent of modification. To further elucidate HS structural organization, the relative position of different HS structures, identified by a set of phage-display-derived anti-HS antibodies, was established. Two strategies were employed: inhibition of HS biosynthesis using 4-deoxy-GlcNAc, followed by resynthesis, and limited degradation of HS using heparinases. Using both approaches, information about the position of antibody-defined HS structures was identified. The HS structure recognized by the antibody NS4F5, rigorously identified as (GlcN6S-IdoA2S)₃, was found towards the non-reducing end of the HS chain.
authors.affiliation: *Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Geert Grooteplein 26-28, 6525 GA Nijmegen, The Netherlands.
authors.forename: Xander M R
authors.initials: XM
authors.lastname: van Wijk
authors.affiliation: *Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Geert Grooteplein 26-28, 6525 GA Nijmegen, The Netherlands.
authors.forename: Arie
authors.initials: A
authors.lastname: Oosterhof
authors.affiliation: *Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Geert Grooteplein 26-28, 6525 GA Nijmegen, The Netherlands.
authors.forename: Elly M M
authors.initials: EM
authors.lastname: Versteeg
authors.affiliation: *Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Geert Grooteplein 26-28, 6525 GA Nijmegen, The Netherlands.
authors.forename: Els M A
authors.initials: EM
authors.lastname: van de Westerlo
authors.affiliation: *Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Geert Grooteplein 26-28, 6525 GA Nijmegen, The Netherlands.
authors.forename: Toin H
authors.initials: TH
authors.lastname: van Kuppevelt
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018604:Epitope Mapping; D005417:Flavobacterium / Q000201:enzymology; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D019763:Heparin Lyase / Q000378:metabolism; D006497:Heparan Sulfate / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D015394:Molecular Structure; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001426:Bacterial Proteins; D003847:Deoxyglucose; D004791:Enzyme Inhibitors; D005944:Glucosamine; D019763:Heparin Lyase; D006497:Heparan Sulfate; C551889:4-deoxyacetylglucosamine