title: Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro.
abstract: The main objective of this study is to investigate the cytotoxic, genotoxic and antioxidant properties of zingiberene (ZBN) in an in vitro rat brain cell culture study. The cytotoxic effect was determined against the rat neuron and N2a neuroblastoma (N2a-NB) cell lines using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, while the antioxidant activity was assessed using the total antioxidant capacity (TAC) and total oxidative stress (TOS) assays. The effects on DNA damage were also evaluated in this study by the single cell gel electrophoresis assay. The results indicated that ZBN has an anti-proliferative activity suppressing the proliferation of N2a-NB cells at concentrations over 50 mg L(-1) and neuron cells at concentrations over 150 mg L(-1). In addition, ZBN treatments at higher doses (≤50 mg L(-1)) led to increases of TOS levels in N2a-NB cell cultures. However 25 mg L(-1) of ZBN treatment caused increases of TAC levels in cultured neuron and N2a-NB cell cultures while ZBN at doses of 10-400 mg L(-1) did not increase the number of total damage score in both cell lines. This study clearly indicates that ZBN has a significant potential to be used as a natural anticancer agent in cultured N2a-NBs.
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey. basaktogar@gmail.com.
authors.forename: Basak
authors.initials: B
authors.lastname: Togar
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Sciences, Erzurum Technical University, Erzurum, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Turkez
authors.affiliation: Department of Medical Genetics, Faculty of Medical, Atatürk University, Erzurum, Turkey.
authors.forename: Abdulgani
authors.initials: A
authors.lastname: Tatar
authors.affiliation: Department of Medical Pharmacology, Faculty of Medical, Atatürk University, Erzurum, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Hacimuftuoglu
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey.
authors.forename: Fatime
authors.initials: F
authors.lastname: Geyikoglu
keywords: MTT assay; N2a neuroblastoma; Single cell gel electrophoresis; Total antioxidant capacity; Total oxidative stress; Zingiberene