title: First description of OXA-48-producing Escherichia coli and the pandemic clone ST131 from patients hospitalised at a military hospital in Algeria.
abstract: The aim of the study was to assess the frequency and diversity of carbapenemases and extended-spectrum β-lactamases (ESBL) produced by Escherichia coli isolates from patients hospitalised in the Regional Military Hospital of Constantine (Algeria). E. coli isolates were collected over a 2-year period from patients presenting E. coli infections. Strains with reduced susceptibility to ertapenem and/or positive for ESBL were characterised with regard to antibiotic resistance, bla genes, phylogenetic groups, O25 serotyping, quinolone resistance, repetitive sequence-based polymerase chain reaction (rep-PCR) profiles and multi-locus sequence typing (MLST). Of the 448 isolated E. coli, 94 (20.9 %) were multidrug-resistant. One of them (1.1 %) produced a bla OXA-48 and was identified as a B1 ST5 strain. The transposon bearing this gene was Tn1999.2. This strain was isolated from a patient coming from a border province with Tunisia, where this carbapenemase is endemic. In addition, 84 (18.8 %) isolates among them produced an ESBL with predominance (97.6 %) of bla CTX-M-15, which was coupled with qnr genes in 10.9 %. ESBL-producing strains were mainly detected in phylogroups D and A. They displayed 20 rep-PCR profiles and all the clonally related isolates were of the same sequence type (ST). Ten strains (9.4 %) belonged to the pandemic clone ST131. This study describes for the first time the presence of OXA-48-producing E. coli and the emergence of the intercontinental ST131 bla CTX-15-producing E. coli strains in Algeria.
authors.affiliation: Laboratoire de Recherche PADESCA, Institut des Sciences Vétérinaires, Université de Constantine 1, Constantine, Algérie.
authors.forename: A
authors.initials: A
authors.lastname: Agabou
authors.forename: A
authors.initials: A
authors.lastname: Pantel
authors.forename: Z
authors.initials: Z
authors.lastname: Ouchenane
authors.forename: N
authors.initials: N
authors.lastname: Lezzar
authors.forename: S
authors.initials: S
authors.lastname: Khemissi
authors.forename: D
authors.initials: D
authors.lastname: Satta
authors.forename: A
authors.initials: A
authors.lastname: Sotto
authors.forename: J-P
authors.initials: JP
authors.lastname: Lavigne
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000462:Algeria; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D004251:DNA Transposable Elements; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006772:Hospitals, Military; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D058889:Molecular Typing; D010802:Phylogeny; D012703:Serotyping; D055815:Young Adult; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D001618:beta-Lactamases; C108449:oxacillinase