title: Preventive and therapeutic effects of a beta adrenoreceptor agonist, dobutamine, in carrageenan-induced inflammatory nociception in rats.
abstract: We hypothesized that locally administrated β-adrenoreceptor agonist can modulate the inflammatory nociceptive parameters in carrageenan (CG)-induced peripheral inflammatory pain. This study was therefore aimed to assess the preventive and therapeutic effects of a β-agonist, dobutamine, by investigating its pretreatment and posttreatment actions on the inflammation-induced hypersensitivities (thermal hyperalgesia, mechanical allodynia) to cutaneous stimulation, edema, and several biochemical oxidant and anti-oxidant parameters in a rat model of CG-induced hind paw inflammation. Effects of dobutamine were compared with those of esmolol, a β-adrenoreceptor antagonist. CG injection to healthy rats lowered the thermal latencies (from 10.1 ± 0.2 to 4.9 ± 0.1 s) and mechanical thresholds (from 32.9 ± 0.5 to 18.9 ± 1.3 g) and caused the hyperalgesia and allodynia. In CG-induced inflamed paws, while intraplantar esmolol treatment (1 mg) produced significant decreases in latencies (4.1 ± 0.1 s) and thresholds (15.2 ± 2.4 g), dobutamine (1 mg) increased the latencies (11.3 ± 0.5 s) and thresholds (26.3 ± 2.8 g). In contrast to esmolol, dobutamine increased the superoxide dismutase level and decreased the myeloperoxidase, malondialdehyde, and nitric oxide levels in CG-induced inflamed paws. The present results can reveal that β-adrenoreceptors may play a role in inflammatory nociceptive processes, and locally treated β-adrenoreceptor agonists such as dobutamine can be a preferable, appropriate choice for the management of inflammatory nociception due to their preventive and therapeutic effects on CG-induced peripheral inflammatory nociception.
authors.affiliation: Department of Biophysics, School of Medicine, Kahramanmaras Sutcu Imam University, 46050, Kahramanmaras, Turkey, tufanmert@yahoo.com.
authors.forename: Tufan
authors.initials: T
authors.lastname: Mert
authors.forename: Berin
authors.initials: B
authors.lastname: Tugtag
authors.forename: Metin
authors.initials: M
authors.lastname: Kilinc
authors.forename: Elif
authors.initials: E
authors.lastname: Sahin
authors.forename: Hafize
authors.initials: H
authors.lastname: Oksuz
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Gunes
mesh_terms: D058665:Adrenergic beta-1 Receptor Agonists / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D002351:Carrageenan / Q000633:toxicity*; D004280:Dobutamine / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D006930:Hyperalgesia / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D008297:Male; D010147:Pain Measurement / Q000187:drug effects / Q000379:methods; D051381:Rats; D017208:Rats, Wistar
chemical_list: D058665:Adrenergic beta-1 Receptor Agonists; D004280:Dobutamine; D002351:Carrageenan