title: Phospholipids and protein adaptation of Pseudomonas sp. to the xenoestrogen tributyltin chloride (TBT).
abstract: A tributyltin (TBT)-resistant strain of Pseudomonas sp. isolated from an overworked car filter was tested for its adaptation to TBT. The isolate was checked for organotin degradation ability, as well as membrane lipid and cellular protein composition in the presence of TBT. The phospholipid profiles of bacteria, grown with and without increased amounts of TBT, were characterized using liquid chromatography/electrospray ionization/mass spectrometry. The strain reacted to the biocide by changing the composition of its phospholipids. TBT induced a twofold decline in the amounts of many molecular species of phosphatidylglycerol and an increase in the levels of phosphatidic acid (by 58%) and phosphatidylethanolamine (by 70%). An increase in the degree of saturation of phospholipid fatty acids of TBT exposed Pseudomonas sp. was observed. These changes in the phospholipid composition and concentration reflect the mechanisms which support optimal lipid ordering in the presence of toxic xenobiotic. In the presence of TBT the abundances of 16 proteins, including TonB-dependent receptors, porins and peroxidases were modified, which could indicate a contribution of some enzymes to TBT resistance.
authors.affiliation: Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237, Lodz, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Bernat
authors.forename: Paulina
authors.initials: P
authors.lastname: Siewiera
authors.forename: Adrian
authors.initials: A
authors.lastname: Soboń
authors.forename: Jerzy
authors.initials: J
authors.lastname: Długoński
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002853:Chromatography, Liquid; D010743:Phospholipids / Q000032:analysis*; D011549:Pseudomonas / Q000737:chemistry* / Q000187:drug effects* / Q000502:physiology; D021241:Spectrometry, Mass, Electrospray Ionization; D013312:Stress, Physiological; D014220:Trialkyltin Compounds / Q000633:toxicity*
chemical_list: D001426:Bacterial Proteins; D010743:Phospholipids; D014220:Trialkyltin Compounds; C011559:tributyltin