title: Expression of matrix metalloproteinase-2 and 9 in cervical intraepithelial neoplasia and cervical carcinoma among different age groups of premenopausal and postmenopausal women.
abstract: OBJECTIVE
  The objective was to study the gelatinolytic activity of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in preinvasive and invasive carcinoma of the uterine cervix. The expressions were analysed against different age groups, as to demonstrate whether the expression of MMP-2 and MMP-9 is an early or a late event during the progression of cervical cancer. Additionally, the diagnostic accuracy of MMP-2 and MMP-9 was evaluated with ROC curve.
  
  
  METHODS
  A total number of 180 samples of cervical tissue were studied for MMP-2 and MMP-9 gelatinolytic activity. The cases were selected as to include 63 normal cases, 94 CIN cases and 23 cervical carcinoma cases. Among 94 CIN cases, 40 were CIN1, 26 were CIN2 and 28 were CIN3, as reported by histopathology. The gelatinolytic activities of MMP-2 and MMP-9 were evaluated by gelatin zymography in premenopausal and postmenopausal groups.
  
  
  RESULTS
  MMP-2 expressions (latent and active) were very low in control samples, followed by increase in CIN1, decrease in CIN2 and further increase in advance stages. MMP-9 had also shown the same expression pattern that of MMP-2. While comparing the expression of MMP-2 and MMP-9 in different age groups, we found initial CIN stages were prevalent in early age that expressed considerable amount of MMP-2 and MMP-9, and advance stages of carcinoma cervix were prevalent at an elderly age.
  
  
  CONCLUSIONS
  Both MMP-2 and MMP-9 have role in cancer progression and remodelling of the ectocervix. Although expression level varies intricately, a distinctive ROC curve demonstrated MMP-2 active form and MMP-9 form could be used in diagnostic purpose in detection of cervical lesion and cancer.
authors.affiliation: Molecular Biology and Human Genetics Laboratory, Department of Zoology, The University of Burdwan, Golapbug Campus, Burdwan, 713101, West Bengal, India.
authors.forename: Arnab
authors.initials: A
authors.lastname: Ghosh
authors.forename: Anuradha
authors.initials: A
authors.lastname: Moirangthem
authors.forename: Rabindranath
authors.initials: R
authors.lastname: Dalui
authors.forename: TapanKumar
authors.initials: T
authors.lastname: Ghosh
authors.forename: Arghya
authors.initials: A
authors.lastname: Bandyopadhyay
authors.forename: Anindya
authors.initials: A
authors.lastname: Dasgupta
authors.forename: Uma
authors.initials: U
authors.lastname: Banerjee
authors.forename: Narayan
authors.initials: N
authors.lastname: Jana
authors.forename: Anupam
authors.initials: A
authors.lastname: Basu
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D017698:Postmenopause / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000235:genetics*
chemical_list: D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9