title: Orthotopic animal model of pseudomyxoma peritonei: An in vivo model to test anti-angiogenic drug effects.
abstract: Pseudomyxoma peritonei (PMP) is an uncommon peritoneal mucinous carcinomatosis confined to the peritoneal cavity. The rarity of PMP in humans makes evaluation of the disease biological features and new therapeutic strategies difficult. Accordingly, there is a need for animal models of PMP. Human PMP tissue was i.p. grafted and grown into nude mice, then constituted into reliable and reproducible orthotopic models. Histological and immunostaining analysis was performed. Bevacizumab was injected twice a week either during tumor growth or after cytoreductive surgery. In vivo imaging of tumor angiogenesis was performed using barium sulfate or isolectin microangiography and Doppler ultrasonography of the superior mesenteric artery. Tumor angiogenesis was confirmed by the presence of tortuous vascular networks with high levels of expression of CD31, vascular endothelial cadherin, and desmin. Doppler ultrasonography of the superior mesenteric artery revealed a twofold increase in blood flow velocity compared with tumor-free mice (P < 0.001). Bevacizumab administration was correlated with the normalization of tumor vascularity when injected during tumor growth and with the stabilization of the histological and hemodynamic findings when injected after cytoreductive surgery. Our PMP models mimic human PMP. Our results confirmed the presence of tumor angiogenesis related to PMP growth. Our murine model allows researchers to actually bench test and evaluate, in preclinical studies, the efficacy of new therapeutic strategies and anti-angiogenic therapies.
authors.affiliation: INSERM U965, C.A.R.T. Carcinomatosis Angiogenesis and Translational Research, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France; Department of Body and Interventional Imaging, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, Assistance Publique - Hôpitaux de Paris, Paris, France. Electronic address: anthony.dohan@lrb.aphp.fr.
authors.forename: Anthony
authors.initials: A
authors.lastname: Dohan
authors.affiliation: INSERM U965, C.A.R.T. Carcinomatosis Angiogenesis and Translational Research, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France.
authors.forename: Ruben
authors.initials: R
authors.lastname: Lousquy
authors.affiliation: INSERM U965, C.A.R.T. Carcinomatosis Angiogenesis and Translational Research, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France; Surgical Oncologic and Digestive Unit, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Eveno
authors.affiliation: Department of Surgical Oncology, Gustave Roussy Institute, Villejuif, France.
authors.forename: Diane
authors.initials: D
authors.lastname: Goere
authors.affiliation: INSERM U965, C.A.R.T. Carcinomatosis Angiogenesis and Translational Research, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France; Blood and Vessels Institute, Paris Diderot University, Sorbonne Paris Cité, Lariboisière Hospital, Paris, France.
authors.forename: Dong
authors.initials: D
authors.lastname: Broqueres-You
authors.affiliation: INSERM U965, C.A.R.T. Carcinomatosis Angiogenesis and Translational Research, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France; Department of Pathology, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France.
authors.forename: Rachid
authors.initials: R
authors.lastname: Kaci
authors.affiliation: Department of Biochemistry and INSERM U944, Paris Diderot University, Sorbonne Paris Cité, Assistance Publique - Hôpitaux de Paris, Saint-Louis Hospital, Paris, France.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Lehmann-Che
authors.affiliation: INSERM U965, C.A.R.T. Carcinomatosis Angiogenesis and Translational Research, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France; Department of Biochemistry, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Launay
authors.affiliation: INSERM U965, C.A.R.T. Carcinomatosis Angiogenesis and Translational Research, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France; Department of Body and Interventional Imaging, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, Assistance Publique - Hôpitaux de Paris, Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Soyer
authors.affiliation: INSERM U965, C.A.R.T. Carcinomatosis Angiogenesis and Translational Research, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France; Department of Clinical Physiology-Functional Investigations, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bonnin
authors.affiliation: INSERM U965, C.A.R.T. Carcinomatosis Angiogenesis and Translational Research, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France; Surgical Oncologic and Digestive Unit, Lariboisière Hospital, Paris Diderot University, Sorbonne Paris Cité, AP-HP, Paris, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Pocard
mesh_terms: D020533:Angiogenesis Inhibitors / Q000627:therapeutic use*; D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000627:therapeutic use; D000068258:Bevacizumab; D001783:Blood Flow Velocity; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008638:Mesenteric Arteries / Q000000981:diagnostic imaging; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D010534:Peritoneal Neoplasms / Q000188:drug therapy*; D011553:Pseudomyxoma Peritonei / Q000188:drug therapy*; D014463:Ultrasonography
chemical_list: D020533:Angiogenesis Inhibitors; D061067:Antibodies, Monoclonal, Humanized; D000068258:Bevacizumab