title: [Effect of paeoniflorin on secretion of TGF-beta1 from macrophages in mice].
abstract: OBJECTIVE
  To explore the effect of paeoniflorin (PAE) on the production of transforming growth factor beta1 (TGF-beta1) from peritoneal macrophages(PMs) stimulated by soluble egg antigen (SEA) of Schistosoma japonicum.
  
  
  METHODS
  SEA was prepared by trituration and added into culture plank, flask and dish containing PMs which were cultured for 24 h. TGF-beta1 secreted from PMs was measured by ELISA. TGF-beta1 mRNA and protein produced from PMs were evaluated by RT-PCR and Western blotting, respectively. SEA (10 mg/L) 5 ml was added into culture flask and dish containing PMs. PMs were cultured for 12 h, and PAE at different concentrations (0, 7.5, 15, 30, 60, 120 mg/L) was added into the culture flask and dish, and PMs were cultured consecutively for another 12 h and 24 h, respectively. TGF-beta1 mRNA and protein from PMs stimulated by SEA were evaluated by RT-PCR and Western blotting, respectively.
  
  
  RESULTS
  TGF-beta1 (235.86 +/- 3.43 ng/L) was produced from PMs under stimulation of SEA at 10 mg/L, and the expression of TGF-beta1 mRNA and protein in PMs were depressed significantly by PAE in a concentration-dependent manner (r = -0.827, P < 0.01; r = -0.952, P < 0.01, respectively).
  
  
  CONCLUSIONS
  PAE inhibits the production of TGF-beta1 from PMs stimulated by SEA.
authors.forename: De-Yong
authors.initials: DY
authors.lastname: Chu
authors.forename: Cong-Lei
authors.initials: CL
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Luo
authors.forename: Mei-Juan
authors.initials: MJ
authors.lastname: Zheng
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wu
authors.forename: Qing-Li
authors.initials: QL
authors.lastname: Luo
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Shen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005960:Glucosides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism / Q000469:parasitology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D039821:Monoterpenes / Q000494:pharmacology*; D012549:Schistosoma japonicum; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D006027:Glycosides
chemical_list: D005960:Glucosides; D039821:Monoterpenes; D053773:Transforming Growth Factor beta1; C015423:peoniflorin; D006027:Glycosides