title: Engineering genetic circuits that compute and remember.
abstract: Memory and logic are central to complex state-dependent computing, and state-dependent behaviors are a feature of natural biological systems. Recently, we created a platform for integrated logic and memory by using synthetic gene circuits, and we demonstrated the implementation of all two-input logic gates with memory in living cells. Here we provide a detailed protocol for the construction of two-input Boolean logic functions with concomitant DNA-based memory. This technology platform allows for straightforward assembly of integrated logic-and-memory circuits that implement desired behaviors within a couple of weeks. It should enable the encoding of advanced computational operations in living cells, including sequential-logic and biological-state machines, for a broad range of applications in biotechnology, basic science and biosensing.
authors.affiliation: 1] Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3] Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [4].
authors.forename: Piro
authors.initials: P
authors.lastname: Siuti
authors.affiliation: 1] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3].
authors.forename: John
authors.initials: J
authors.lastname: Yazbek
authors.affiliation: 1] Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3] Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Timothy K
authors.initials: TK
authors.lastname: Lu
mesh_terms: D016248:Computer Storage Devices; D039301:Computers, Molecular; D005818:Genetic Engineering / Q000379:methods*; D045522:Recombinases / Q000737:chemistry*
chemical_list: D045522:Recombinases