title: Biodegradation of biphenyl and 2-chlorobiphenyl by a Pseudomonas sp. KM-04 isolated from PCBs-contaminated coal mine soil.
abstract: The biphenyl-degrading strain, Pseudomonas sp. KM-04, was isolated from polychlorinated biphenyls-contaminated soil sample obtained from the vicinity of a former coal mine. We herein report that strain KM-04 can use biphenyl as a sole carbon source, and resting cells convert biphenyl to its corresponding metabolic intermediates. Incubation of KM-04 with autoclaved mining-contaminated soil for 10 days in a slurry system reduced the levels of biphenyl and 2-chlorobiphenyl by 98.5 % and 82.3 %, respectively. Furthermore, treatment of a mine-soil microcosm with strain KM-04 for 15 days in a composting system under laboratory conditions reduced the levels of biphenyl and 2-chlorobiphenyl by 87.1 % and 68.7 %, respectively. These results suggest that KM-04 is a potential candidate for the biological removal of biphenyl and its chlorinated derivatives from polychlorinated biphenyl-contaminated mining areas.
authors.affiliation: Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources (KIGAM), 92 Gwahang-no, Yusung-gu, Daejeon, 305-350, Korea, nih@kigam.re.kr.
authors.forename: In-Hyun
authors.initials: IH
authors.lastname: Nam
authors.forename: Chul-Min
authors.initials: CM
authors.lastname: Chon
authors.forename: Ka-Young
authors.initials: KY
authors.lastname: Jung
authors.forename: Jae-Gon
authors.initials: JG
authors.lastname: Kim
mesh_terms: D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000032:analysis / Q000378:metabolism*; D003031:Coal; D008906:Mining; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D001713:Biphenyl Compounds; D003031:Coal; D012987:Soil; D012989:Soil Pollutants; C055142:2-chlorobiphenyl; C010574:biphenyl