title: Molecular detection of bacteria associated to dental caries in 4-12-year-old Tunisian children.
abstract: The occurrence of several microbial species in the oral cavity of 4-12-year-old Tunisian children was investigated. Samples were taken from 158 children (81 caries actives and 77 caries free). Genomic DNA was extracted and analyzed for the presence of 17 microbial species using a polymerase chain reaction assay. All samples were positive for at least one of the target microbial strains. Streptococcus mutans was the most prevalent species (76.5%) detected in genomic DNA collected from carious lesions. Other prevalent species were Candida spp (63%), Streptococcus salivarius (59%) and Streptococcus oralis (42%). The frequency of Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus casei-group in caries lesions was 29.5%, 34.5% and 22% respectively. Pathogenic bacteria such as Staphylococcus aureus was found in 28.5% of carious lesion samples compared to 15.5% in the control. Frequency of Porphyromonas endodontali, Actinomyces radicidentis and Treponema denticola recovery did not differ significantly between origins of samples. PCR analysis of genomic DNA detect various oral bacteria that differ between caries actives and caries-free children. In addition, the association of same aciduric bacteria (S. mutans, S. salivarius, L. acidophilus) and caries formation was noticed.
authors.affiliation: College of Applied Medical Sciences, Yanbu el Bahr, Taibah University, 5000, Saudi Arabia. Electronic address: bochrak@yahoo.fr.
authors.forename: Bochra
authors.initials: B
authors.lastname: Kouidhi
authors.affiliation: Laboratory of Analyze, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia.
authors.forename: Kais
authors.initials: K
authors.lastname: Fdhila
authors.affiliation: Laboratory of Analyze, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia.
authors.forename: Rihab
authors.initials: R
authors.lastname: Ben Slama
authors.affiliation: Laboratory of Molecular Biology, Ibn el Jazar Hospital, Kairouan, Monastir University, Tunisia.
authors.forename: Kacem
authors.initials: K
authors.lastname: Mahdouani
authors.affiliation: Department of Medicine and Oral Surgery, University Hospital, Clinic of Odontology, Monastir University, Tunisia.
authors.forename: Hajer
authors.initials: H
authors.lastname: Hentati
authors.affiliation: Department of Medicine and Oral Surgery, University Hospital, Clinic of Odontology, Monastir University, Tunisia.
authors.forename: Fayrouz
authors.initials: F
authors.lastname: Najjari
authors.affiliation: Laboratory of Analyze, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia.
authors.forename: Amina
authors.initials: A
authors.lastname: Bakhrouf
authors.affiliation: College of Sciences, Yanbu el Bahr, Taibah University, Saudi Arabia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Chaieb
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D044822:Biodiversity; D002175:Candida / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D003731:Dental Caries / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal
keywords: Children; Dental caries; Genomic DNA; Oral bacteria; PCR; Streptococcus mutans