title: Production of lycopene by metabolically-engineered Escherichia coli.
abstract: Escherichia coli strain CAR001 that produces β-carotene was genetically engineered to produce lycopene by deleting genes encoding zeaxanthin glucosyltransferase (crtX) and lycopene β-cyclase (crtY) from the crtEXYIB operon. The resulting strain, LYC001, produced 10.5 mg lycopene/l (6.5 mg/g dry cell weight, DCW). Modulating expression of genes encoding α-ketoglutarate dehydrogenase, succinate dehydrogenase and transaldolase B within central metabolic modules increased NADPH and ATP supplies, leading to a 76 % increase of lycopene yield. Ribosome binding site libraries were further used to modulate expression of genes encoding 1-deoxy-D-xylulose-5-phosphate synthase (dxs) and isopentenyl diphosphate isomerase (idi) and the crt gene operon, which improved the lycopene yield by 32 %. The optimal strain LYC010 produced 3.52 g lycopene/l (50.6 mg/g DCW) in fed-batch fermentation.
authors.affiliation: College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China, suntao23beckham@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Liangtian
authors.initials: L
authors.lastname: Miao
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Li
authors.forename: Guanping
authors.initials: G
authors.lastname: Dai
authors.forename: Fuping
authors.initials: F
authors.lastname: Lu
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Xueli
authors.initials: X
authors.lastname: Zhang
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D002338:Carotenoids / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D000077276:Lycopene; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics
chemical_list: D002338:Carotenoids; D000077276:Lycopene