title: [Analysis of capsule related surface properties of Porphyromonas gingivalis isolates].
abstract: OBJECTIVE
  To analyze the capsule related surface properties of Porphyromonas gingivalis (Pg) isolates.
  
  
  METHODS
  The transmission electron microscopy (TEM) was used to observe the capsule structure and the capsule thickness of 2 type of strains and 5 clinical isolates. Microbial adhesion to hydrocarbons (MATH) assay was used to qualitatively assess the hydrophobicity of each strain, and the capacities of these strains were investigated by autoaggregation assay.Ninety-six well biofilm assay and confocal laser scanning microscopy (CLSM) were applied to quantify and observe the biofilm produced by each strain.
  
  
  RESULTS
  TEM showed the variety of capsule thickness of these strains.Virulent type strain W83 possessed thicker capsular structure than less-virulent type strain ATCC33277. The SJD4 possessed thicker capsule than other clinical isolates, followed by SJD11, SJD5, SJD2, and SJD12.Strains W83, SJD4, SJD11, with thicker capsule, were much more hydrophilic with lower MATH percentage, in accordance with a slow autoaggregation in incubation during a period of 240 min. Compared with W83, the hydrophobicity of strains ATCC33277, SJD5, SJD2, and SJD12, with thinner capsule, showed increased MATH percentage and autoaggregations. All clinical strains developed biofilm with different absorbance compared with type strains. The CLSM observation showed biofilm thickness of each strain, ranged from (14.74 ± 4.99) to (24.13 ± 5.45) µm. Strain W83 and SJD11 showed notable poor biofilm formation, while others developed dense and mature biofilm.
  
  
  CONCLUSIONS
  There was a certain degree of linkage between the Pg capsule thickness and surface properties diversity.
authors.affiliation: Department of Periodontology, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine & Shanghai Research Institute of Stomatology & Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Periodontology, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine & Shanghai Research Institute of Stomatology & Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
authors.forename: Dali
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Periodontology, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine & Shanghai Research Institute of Stomatology & Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Periodontology, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine & Shanghai Research Institute of Stomatology & Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Periodontology, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine & Shanghai Research Institute of Stomatology & Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. Email: shurong123@hotmail.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Shu
mesh_terms: D016667:Bacterial Capsules; D018441:Biofilms; D057927:Hydrophobic and Hydrophilic Interactions; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D013499:Surface Properties