title: Agrobacterium-mediated transformation of barley (Hordeum vulgare L.).
abstract: Barley biotechnology requires efficient genetic engineering tools for producing transgenic plants necessary for conducting reverse genetics analyses in breeding and functional genomics research. Agrobacterium-mediated genetic transformation is an important technique for producing barley transgenics with simple low-copy number transgenes. This chapter reports a refined protocol for the systematic high-throughput transformation of the advanced Australian spring barley breeding line WI4330.
authors.affiliation: Australian Centre for Plant Functional Genomics, University of Adelaide, PMB 1, Glen Osmond, SA, Australia.
authors.forename: Ainur
authors.initials: A
authors.lastname: Ismagul
authors.forename: Iryna
authors.initials: I
authors.lastname: Mazonka
authors.forename: Corinne
authors.initials: C
authors.lastname: Callegari
authors.forename: Serik
authors.initials: S
authors.lastname: Eliby
mesh_terms: D060054:Agrobacterium / Q000235:genetics*; D017343:Genes, Plant; D005818:Genetic Engineering; D001467:Hordeum / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic