title: Characterization of silk fibroin modified surface: a proteomic view of cellular response proteins induced by biomaterials.
abstract: The purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profiling system. The surface was covered by multiwalled carbon nanotubes (CNTs) and SF to increase the surface area, enhance the adhesion of biopolymer, and promote the rate of cell proliferation. The amount of adhered fibroblasts on CNTs/SF electrodes of quartz crystal microbalance (QCM) greatly exceeded those on other surfaces. Moreover, analyzing differential protein expressions of adhered fibroblasts on the biopolymer surface by proteomic approaches indicated that CD44 may be a key protein. Through this study, utilization of mass spectrometry-based proteomics in evaluation of cell adhesion on biopolymer was proposed.
authors.affiliation: Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 300, Taiwan ; Department of Medical Research, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan ; Translational Research Center, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan.
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Yang
authors.affiliation: Department of Medical Research, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan ; Translational Research Center, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan ; Department of Obstetrics and Gynecology, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan ; School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Shyng-Shiou
authors.initials: SS
authors.lastname: Yuan
authors.affiliation: Department of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan.
authors.forename: Tze-Wen
authors.initials: TW
authors.lastname: Chung
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; Department of Nuclear Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan.
authors.forename: Shiang-Bin
authors.initials: SB
authors.lastname: Jong
authors.affiliation: Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; National Sun Yat-Sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 804, Taiwan.
authors.forename: Chi-Yu
authors.initials: CY
authors.lastname: Lu
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Wan-Chi
authors.initials: WC
authors.lastname: Tsai
authors.affiliation: Department of Fiber and Composite Materials, College of Engineering, Feng Chia University, Taichung 407, Taiwan.
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Chen
authors.affiliation: National Sun Yat-Sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 804, Taiwan ; Department of Chemistry, National Sun Yat-Sen University, Kaohsiung 804, Taiwan.
authors.forename: Po-Chiao
authors.initials: PC
authors.lastname: Lin
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Pei-Wen
authors.initials: PW
authors.lastname: Chiang
authors.affiliation: Translational Research Center, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan ; Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; National Sun Yat-Sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 804, Taiwan ; Center of Biomedical Engineering and System Biology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Tyan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004566:Electrodes; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005349:Fibroins / Q000494:pharmacology*; D063990:Gene Ontology; D018960:Hyaluronan Receptors / Q000378:metabolism; D007150:Immunohistochemistry; D013058:Mass Spectrometry; D018625:Microscopy, Atomic Force; D037742:Nanotubes, Carbon / Q000737:chemistry; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D058525:Quartz Crystal Microbalance Techniques; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D018960:Hyaluronan Receptors; D037742:Nanotubes, Carbon; D011506:Proteins; D005349:Fibroins; D001973:Bromodeoxyuridine