title: U-251 revisited: genetic drift and phenotypic consequences of long-term cultures of glioblastoma cells.
abstract: It is well known that in vitro subculture represents a selection pressure on cell lines, and over time this may result in a genetic drift in the cancer cells. In addition, long-term cultures harbor the risk of cross-contamination with other cell lines. The consequences may have major impact on experimental results obtained in various laboratories, where the cell lines no longer reflect the original tumors that they are supposed to represent. Much neglected in the scientific community is a close monitoring of cell cultures by regular phenotypic and genetic characterization. In this report, we present a thorough characterization of the commonly used glioblastoma (GBM) model U-251, which in numerous publications has been wrongly identified as U-373, due to an earlier cross-contamination. In this work, the original U-251 and three subclones of U-251, commonly referred to as U-251 or U-373, were analyzed with regard to their DNA profile, morphology, phenotypic expression, and growth pattern. By array comparative genomic hybridization (aCGH), we show that only the original low-passaged U-251 cells, established in the 1960s, maintain a DNA copy number resembling a typical GBM profile, whereas all long-term subclones lost the typical GBM profile. Also the long-term passaged subclones displayed variations in phenotypic marker expression and showed an increased growth rate in vitro and a more aggressive growth in vivo. Taken together, the variations in genotype and phenotype as well as differences in growth characteristics may explain different results reported in various laboratories related to the U-251 cell line.
authors.affiliation: Department of Biomedicine, University of Bergen, Bergen, Norway.
authors.forename: Anja
authors.initials: A
authors.lastname: Torsvik
authors.forename: Daniel
authors.initials: D
authors.lastname: Stieber
authors.forename: Per Øyvind
authors.initials: PØ
authors.lastname: Enger
authors.forename: Anna
authors.initials: A
authors.lastname: Golebiewska
authors.forename: Anders
authors.initials: A
authors.lastname: Molven
authors.forename: Agnete
authors.initials: A
authors.lastname: Svendsen
authors.forename: Bengt
authors.initials: B
authors.lastname: Westermark
authors.forename: Simone P
authors.initials: SP
authors.lastname: Niclou
authors.forename: Thale Kristin
authors.initials: TK
authors.lastname: Olsen
authors.forename: Martha
authors.initials: M
authors.lastname: Chekenya Enger
authors.forename: Rolf
authors.initials: R
authors.lastname: Bjerkvig
mesh_terms: D000818:Animals; D063646:Carcinogenesis; D045744:Cell Line, Tumor / Q000502:physiology*; D049109:Cell Proliferation; D048430:Cell Shape; D055028:Comparative Genomic Hybridization; D005260:Female; D015870:Gene Expression; D040961:Genetic Drift; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D059785:Karyotype; D008297:Male; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D010641:Phenotype; D011003:Ploidies
keywords: GBM; STR; U251; U373; aCGH; cell lines; cross-contamination; in vitro models