title: One-pot and one-step synthesis of bioactive urease/ZnFe₂O₄ nanocomposites and their application in detection of urea.
abstract: This communication describes a novel environmentally friendly method to prepare bioactive urease/ZnFe2O4 nanocomposites through a one-pot and one-step process. The synthetic procedure is triggered through a biological mineralization process of decomposition of urea catalyzed by urease. During the growth of ZnFe2O4, urease molecules are immobilized by original ZnFe2O4 nanoparticles. As a consequence, the bioactive urease/ZnFe2O4 nanoparticle composites are assembled. This simple route is expected to endow the bioactive nanocomposites with new properties for various interesting fields.
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Research Center for Micro&Nano Materials and Technology, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China. mengxw@mail.ipc.ac.cn.
authors.forename: Haitang
authors.initials: H
authors.lastname: Shi
authors.forename: Xue
authors.initials: X
authors.lastname: Chen
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
authors.forename: Longfei
authors.initials: L
authors.lastname: Tan
authors.forename: Xiangling
authors.initials: X
authors.lastname: Ren
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Xianwei
authors.initials: X
authors.lastname: Meng
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods; D001962:Bromcresol Purple / Q000737:chemistry; D002138:Calibration; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008956:Models, Chemical; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D014508:Urea / Q000737:chemistry*; D014510:Urease / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D001962:Bromcresol Purple; D014508:Urea; D014510:Urease; D015032:Zinc