title: Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis.
abstract: We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN γ and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient's clinical condition. Colombian ocular toxoplasmosis patients' immune response was Th2-skewed, regardless of the nature of antigen stimulus.
authors.affiliation: Grupo de Estudio en Parasitología Molecular (GEPAMOL), Facultad de Ciencias de la Salud, Centro de Investigaciones Biomédicas, Universidad del Quindio, Av. Bolivar 12 N, Armenia, Quindio, Colombia, South America.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Torres-Morales
authors.forename: Laura
authors.initials: L
authors.lastname: Taborda
authors.forename: Nestor
authors.initials: N
authors.lastname: Cardona
authors.forename: Alejandra
authors.initials: A
authors.lastname: De-la-Torre
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Sepulveda-Arias
authors.forename: Manuel Alfonso
authors.initials: MA
authors.lastname: Patarroyo
authors.forename: Jorge Enrique
authors.initials: JE
authors.lastname: Gomez-Marin
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D000953:Antigens, Protozoan / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology; D049109:Cell Proliferation; D002648:Child; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014123:Toxoplasmosis / Q000276:immunology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D000953:Antigens, Protozoan; D016207:Cytokines; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma; D017346:Protein Serine-Threonine Kinases; C556793:ROP18 protein, Toxoplasma gondii