title: Nontargeted detection of adulteration of skim milk powder with foreign proteins using UHPLC-UV.
abstract: Chromatographic profiles of skim milk powder (SMP) and mixtures of SMP with soy (SPI), pea (PPI), brown rice (BRP), and hydrolyzed wheat protein (HWPI) isolates were obtained by ultrahigh-performance liquid chromatography (UHPLC) with 215 nm detection. Two data analysis approaches were compared for their utility to classify samples as authentic or adulterated. The t test approach evaluated data points exceeding the 99% confidence limit of the mean authentic SMP chromatogram and used data points from the entire chromatogram. The other approach used the multivariate Q statistic from a SIMCA model of authentic samples to determine adulteration and used a selected retention window to obtain best classifications. Q-Statistic and t test correctly classified adulteration of SMP with SPI at the 1% and 3% levels, respectively, while minimizing false classifications of authentic SMP. Detection of SMP adulterated with PPI, BRP, and HWPI was possible at higher adulteration levels.
authors.affiliation: Center for Food Safety and Applied Nutrition, Division of Food Processing Science and Technology, U.S. Food and Drug Administration , 6502 South Archer Road, Bedford Park, Illinois 60501, United States.
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Jablonski
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Moore
authors.forename: James M
authors.initials: JM
authors.lastname: Harnly
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry; D018532:Pisum sativum / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis*; D011208:Powders / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry; D014908:Triticum / Q000737:chemistry
chemical_list: D010940:Plant Proteins; D011208:Powders