title: [Biodiversity and enzymes of culturable facultative-alkaliphilic actinobacteria in saline-alkaline soil in Fukang, Xinjiang].
abstract: OBJECTIVE
  In order to analyze the biodiversity of cultivable facultative-alkaliphilic actinobacteria and the enzymes they produced.
  
  
  METHODS
  Total 10 soil samples were collected from saline-alkaline environments of Fukang, Xinjiang province. Facultative-alkaliphilic actinobacteria strains were isolated and identified by 16S rRNA gene sequence analysis. Enzymes including amylase, proteinase, xylanase, and cellulase were detected.
  
  
  RESULTS
  Total 116 facultative-alkaliphilic actinobacterial strains and 4 alkali-tolerant actinobacterial strains were isolated from the samples, and those strains were distributed within 22 genera in 13 families and 8 orders of actinobacteria based on their 16S rRNA gene sequence analysis. The ratio of non-predominant Streptomyces and Nocardiopsis strains were 53.3%. The positive rates of amylase, proteinase, xylanase and cellulase were 35.8, 37.6, 28.3 and 17.5%, respectively.
  
  
  CONCLUSIONS
  Diverse facultative-alkaliphilic actinobacteria were discovered from saline-alkaline environments of Fukang. Facultative-alkaliphilic actinobacteria are a potential source for enzymes. The study would facilitate the knowledge of the diversity of facultative-alkaliphilic actinobacteria, and provide the technical basis for exploration of facultative-alkaliphilic actinobacteria resources.
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Hongfei
authors.initials: H
authors.lastname: Wang
authors.forename: Daofeng
authors.initials: D
authors.lastname: Zhang
authors.forename: Jiyue
authors.initials: J
authors.lastname: Chen
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D000468:Alkalies / Q000032:analysis / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D044822:Biodiversity; D002681:China; D008969:Molecular Sequence Data; D010802:Phylogeny; D054712:Salinity; D012965:Sodium Chloride / Q000032:analysis / Q000378:metabolism; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D000468:Alkalies; D001426:Bacterial Proteins; D012987:Soil; D012965:Sodium Chloride