title: Noninvasive detection of colorectal cancer by analysis of exhaled breath.
abstract: There has been growing interest in exhaled breath analysis for cancer screening and disease monitoring; however, limited breath biomarker information exists regarding colorectal cancer (CRC). The objective of this study was to screen for breath biomarkers of CRC. Exhaled breath was collected from 20 CRC patients and 20 healthy controls; subsequently, solid-phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS) was used to assess the exhaled volatile organic compounds (VOCs) of the study participants. The statistical methods of principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to process the final data. The VOCs in the exhalations of CRC patients exhibited significant differences from the VOCs in the exhalations of healthy controls; in particular, relative to the latter exhalations, the former exhalations contain significantly higher levels of cyclohexanone, 2,2-dimethyldecane, dodecane, 4-ethyl-1-octyn-3-ol, ethylaniline, cyclooctylmethanol, trans-2-dodecen-1-ol, and 3-hydroxy-2,4,4-trimethylpentyl 2-methylpropanoate but significantly lower levels of 6-t-butyl-2,2,9,9-tetramethyl-3,5-decadien-7-yne (P < 0.05). Analyses of breath VOCs provide a related model of CRC exhalation that could represent an effective and convenient screening method for this disease.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng St, Nangang District, 150001, Harbin, China.
authors.forename: Changsong
authors.initials: C
authors.lastname: Wang
authors.forename: Chaofu
authors.initials: C
authors.lastname: Ke
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Wang
authors.forename: Chunjie
authors.initials: C
authors.lastname: Chi
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Suqi
authors.initials: S
authors.lastname: Luo
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Guo
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
authors.forename: Fengmin
authors.initials: F
authors.lastname: Zhang
authors.forename: Enyou
authors.initials: E
authors.lastname: Li
mesh_terms: D001944:Breath Tests / Q000379:methods*; D015179:Colorectal Neoplasms / Q000175:diagnosis*; D016002:Discriminant Analysis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D025341:Principal Component Analysis; D052617:Solid Phase Microextraction / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D055549:Volatile Organic Compounds