title: An integrated map of HIV-human protein complexes that facilitate viral infection.
abstract: Recent proteomic and genetic studies have aimed to identify a complete network of interactions between HIV and human proteins and genes. This HIV-human interaction network provides invaluable information as to how HIV exploits the host machinery and can be used as a starting point for further functional analyses. We integrated this network with complementary datasets of protein function and interaction to nominate human protein complexes with likely roles in viral infection. Based on our approach we identified a global map of 40 HIV-human protein complexes with putative roles in HIV infection, some of which are involved in DNA replication and repair, transcription, translation, and cytoskeletal regulation. Targeted RNAi screens were used to validate several proteins and complexes for functional impact on viral infection. Thus, our HIV-human protein complex map provides a significant resource of potential HIV-host interactions for further study.
authors.affiliation: Departments of Medicine and Bioengineering, University of California at San Diego, La Jolla, California, United States of America; IP&Science, Thomson Reuters Scientific Inc., Carlsbad, California, United States of America.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Emig-Agius
authors.affiliation: Sanford-Burnham Medical Research Institute, La Jolla, California, United States of America.
authors.forename: Kevin
authors.initials: K
authors.lastname: Olivieri
authors.affiliation: Sanford-Burnham Medical Research Institute, La Jolla, California, United States of America.
authors.forename: Lars
authors.initials: L
authors.lastname: Pache
authors.affiliation: Sanford-Burnham Medical Research Institute, La Jolla, California, United States of America.
authors.forename: Hsin Ling
authors.initials: HL
authors.lastname: Shih
authors.affiliation: IP&Science, Thomson Reuters Scientific Inc., Carlsbad, California, United States of America.
authors.forename: Olga
authors.initials: O
authors.lastname: Pustovalova
authors.affiliation: IP&Science, Thomson Reuters Scientific Inc., Carlsbad, California, United States of America.
authors.forename: Marina
authors.initials: M
authors.lastname: Bessarabova
authors.affiliation: The Salk Institute for Biological Studies, La Jolla, California, United States of America.
authors.forename: John A T
authors.initials: JA
authors.lastname: Young
authors.affiliation: Sanford-Burnham Medical Research Institute, La Jolla, California, United States of America.
authors.forename: Sumit K
authors.initials: SK
authors.lastname: Chanda
authors.affiliation: Departments of Medicine and Bioengineering, University of California at San Diego, La Jolla, California, United States of America.
authors.forename: Trey
authors.initials: T
authors.lastname: Ideker
mesh_terms: D057809:HEK293 Cells; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D054884:Host-Pathogen Interactions; D054298:Human Immunodeficiency Virus Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods; D034622:RNA Interference; D015398:Signal Transduction
chemical_list: D054298:Human Immunodeficiency Virus Proteins