title: KLF13 cooperates with c-Maf to regulate IL-4 expression in CD4+ T cells.
abstract: Kruppel-like factor (KLF) 13 is a transcription factor that positively regulates expression of the chemokine RANTES 3-5 d after activation of T cells. In this study, we document a key role for KLF13 in the expression of IL-4 in CD4(+) T cells. Gene expression analysis in activated T cells from Klf13(-/-) mice showed that IL-4, along with other Th2 cytokine genes, was downregulated when compared with cells from wild-type mice. The decreased levels of IL-4 were not associated with changes in expression of the Th2-inducing transcription factors GATA3 or c-Maf. Additional analysis revealed that KLF13 directly binds to IL-4 promoter regions and synergizes with c-Maf to positively regulate IL-4 expression. These results indicate that KLF13 is a positive regulator for differentiation of Th2 cells, as part of the transcriptional machinery that regulates IL-4 production in Th2 cells.
authors.affiliation: Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892;
authors.forename: Seok Joo
authors.initials: SJ
authors.lastname: Kwon
authors.affiliation: Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892;
authors.forename: Juan
authors.initials: J
authors.lastname: Crespo-Barreto
authors.affiliation: Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892;
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892;
authors.forename: Tianhong
authors.initials: T
authors.lastname: Wang
authors.affiliation: Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892;
authors.forename: Dong Seok
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892; Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611; and Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611.
authors.forename: Alan
authors.initials: A
authors.lastname: Krensky
authors.affiliation: Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892; Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611; and Clayberger@northwestern.edu.
authors.forename: Carol
authors.initials: C
authors.lastname: Clayberger
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology*; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology*; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000276:immunology*; D051257:Proto-Oncogene Proteins c-maf / Q000235:genetics / Q000276:immunology*; D012097:Repressor Proteins / Q000235:genetics / Q000276:immunology*; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology*; D014158:Transcription, Genetic / Q000235:genetics / Q000276:immunology
chemical_list: D018797:Cell Cycle Proteins; C486422:Klf13 protein, mouse; D051741:Kruppel-Like Transcription Factors; C495608:Maf protein, mouse; D051257:Proto-Oncogene Proteins c-maf; D012097:Repressor Proteins; D015847:Interleukin-4