title: Sleep and movement differentiates actions of two types of somatostatin-expressing GABAergic interneuron in rat hippocampus.
abstract: Neuropeptides acting on pre- and postsynaptic receptors are coreleased with GABA by interneurons including bistratified and O-LM cells, both expressing somatostatin but innervating segregated dendritic domains of pyramidal cells. Neuropeptide release requires high-frequency action potentials, but the firing patterns of most peptide/GABA-releasing interneurons during behavior are unknown. We show that behavioral and network states differentiate the activities of bistratified and O-LM cells in freely moving rats. Bistratified cells fire at higher rates during sleep than O-LM cells and, unlike O-LM cells, strongly increase spiking during sharp wave-associated ripples (SWRs). In contrast, O-LM interneurons decrease firing during sleep relative to awake states and are mostly inhibited during SWRs. During movement, both cell types fire cooperatively at the troughs of theta oscillations but with different frequencies. Somatostatin and GABA are differentially released to distinct dendritic zones of CA1 pyramidal cells during sleep and wakefulness to coordinate segregated glutamatergic inputs from entorhinal cortex and CA3.
authors.affiliation: MRC Anatomical Neuropharmacology Unit, Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3TH, UK. Electronic address: linda.katona@pharm.ox.ac.uk.
authors.forename: Linda
authors.initials: L
authors.lastname: Katona
authors.affiliation: MRC Anatomical Neuropharmacology Unit, Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3TH, UK.
authors.forename: Damien
authors.initials: D
authors.lastname: Lapray
authors.affiliation: MRC Anatomical Neuropharmacology Unit, Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3TH, UK.
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Viney
authors.affiliation: Institute of Public Health, College of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, P.O. Box 17666, United Arab Emirates.
authors.forename: Abderrahim
authors.initials: A
authors.lastname: Oulhaj
authors.affiliation: Center for Brain Research, Medical University of Vienna, Spitalgasse 4, Vienna, A-1090, Austria.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Borhegyi
authors.affiliation: MRC Anatomical Neuropharmacology Unit, Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3TH, UK.
authors.forename: Benjamin R
authors.initials: BR
authors.lastname: Micklem
authors.affiliation: MRC Anatomical Neuropharmacology Unit, Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3TH, UK; Center for Brain Research, Medical University of Vienna, Spitalgasse 4, Vienna, A-1090, Austria. Electronic address: thomas.klausberger@meduniwien.ac.at.
authors.forename: Thomas
authors.initials: T
authors.lastname: Klausberger
authors.affiliation: MRC Anatomical Neuropharmacology Unit, Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3TH, UK; Center for Brain Research, Medical University of Vienna, Spitalgasse 4, Vienna, A-1090, Austria. Electronic address: peter.somogyi@pharm.ox.ac.uk.
authors.forename: Peter
authors.initials: P
authors.lastname: Somogyi
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology*; D007395:Interneurons / Q000502:physiology*; D008297:Male; D009068:Movement / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep / Q000502:physiology*; D013004:Somatostatin / Q000378:metabolism*; D014851:Wakefulness; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: C070614:neurobiotin; D013004:Somatostatin; D005680:gamma-Aminobutyric Acid; D001710:Biotin