title: [Separation and identification of the surface and secreted proteins in Toxoplasma gondii by biotin labeling and proteomics methods].
abstract: OBJECTIVE
  To separate and identify the surface proteins and secreted proteins of Toxoplasma gondii tachyzoites of RH strain.
  
  
  METHODS
  T. gondii tachyzoites were cultured in Vero cells, and purified by filtration and Percoll cell separation solution. The biotin-labeled tachyzoites were lysed, and the surface and secreted proteins were separated by NeutrAvidin agarose beads. After condensation and SDS-PAGE, the protein were collected, digested and identified by LC/MS-MS.
  
  
  RESULTS
  A total of 785 T. gondii proteins were identified, 81 (10.3%) of which were originally annotated as the surface or secreted proteins. Among the highly-expressed (PSM>10) 65 proteins, 43 (66%) were originally annotated as surface or secreted proteins, while the others were predicted unknown proteins.
  
  
  CONCLUSIONS
  The surface and secreted proteins of T. gondii are separated by biotin labeling and avidin chromatography, among which some potential new surface or secreted proteins of T. gondii are identified.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Min-Jun
authors.initials: MJ
authors.lastname: Huang
authors.forename: Shao-Bo
authors.initials: SB
authors.lastname: Gan
authors.forename: Jun-Chao
authors.initials: JC
authors.lastname: Gu
mesh_terms: D000818:Animals; D001710:Biotin; D002522:Chlorocebus aethiops; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015800:Protozoan Proteins / Q000302:isolation & purification / Q000378:metabolism; D014122:Toxoplasma / Q000378:metabolism*; D014709:Vero Cells
chemical_list: D008565:Membrane Proteins; D015800:Protozoan Proteins; D001710:Biotin