title: Plate-based assay for swimming motility in Pseudomonas aeruginosa.
abstract: Swimming motility is a flagellum-dependent form of movement observed in the Gram-negative bacterium Pseudomonas aeruginosa. Swimming motility is defined as the movement in liquid or low-viscosity conditions (up to 0.3 % agar concentration). Unlike swarming motility, swimming motility requires a functional flagellum, but neither quorum sensing (QS) systems nor biosurfactants. While swimming motility can also be observed via microscopy, here we describe a reproducible plate-based method.
authors.affiliation: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
authors.forename: Dae-Gon
authors.initials: DG
authors.lastname: Ha
authors.forename: Sherry L
authors.initials: SL
authors.lastname: Kuchma
authors.forename: George A
authors.initials: GA
authors.lastname: O'Toole
mesh_terms: D001681:Biological Assay / Q000379:methods*; D009068:Movement; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D053038:Quorum Sensing