title: Expression of angiotensin-converting enzyme gene in whole blood in patients with essential hypertension.
abstract: OBJECTIVE
  The present study aims to investigate the correlation of the angiotensin-converting enzyme (ACE) gene expression and protein expression in patients with essential hypertension in whole blood.
  
  
  METHODS
  ACE gene expression was analyzed by Real Time PCR and western blot in 52 patients with essential hypertension and 42 healthy controls.
  
  
  RESULTS
  We observed a significant increase in Delta threshold cycle (ΔCT) values in the circulating ACE gene and ACE protein expression in patients as compared to controls.
  
  
  CONCLUSIONS
  The up-regulation in relative expression of circulating Angiotensin converting enzyme mRNA and protein in patients with respect to controls might be correlated with high blood pressure in patients with essential hypertension.
authors.affiliation: Dr. B R Ambedkar Centre for Biomedical Research, University of Delhi , Delhi , India .
authors.forename: Sudhir
authors.initials: S
authors.lastname: Chandra
authors.forename: Rajiv
authors.initials: R
authors.lastname: Narang
authors.forename: Daman
authors.initials: D
authors.lastname: Saluja
authors.forename: Jagriti
authors.initials: J
authors.lastname: Bhatia
authors.forename: Kamna
authors.initials: K
authors.lastname: Srivastava
mesh_terms: D000328:Adult; D001483:Base Sequence; D015153:Blotting, Western; D016022:Case-Control Studies; D017931:DNA Primers; D005260:Female; D006801:Humans; D006973:Hypertension / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000097:blood / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D007703:Peptidyl-Dipeptidase A
keywords: Angiotensin-converting enzyme; blood pressure; essential hypertension; gene expression; renin–angiotensin–aldosterone system