title: Human S100A9 potentiates IL-8 production in response to GM-CSF or fMLP via activation of a different set of transcription factors in neutrophils.
abstract: Inflammation is highly regulated by various agents. Unexpectedly, we report here that the damage-associated molecular pattern S100A9 protein, a potent neutrophil activator and inducer of cytokine production in monocytes, is not a direct activator of cytokine production in human neutrophils. However, S100A9 primed IL-8 production in fMLP- and GM-CSF-stimulated neutrophiles via NF-κB and CREB-1, and NF-κB, STAT3 and STAT5, respectively. Pharmacological inhibition confirmed the importance of these transcription factors by significantly decreasing IL-8 production. This is the first time that a different set of transcription factors are shown to be involved in S100A9-primed neutrophils in response to proinflammatory agonist.
authors.affiliation: Laboratoire de Recherche en Inflammation et Physiologie des Granulocytes, Université du Québec, Canada.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Simard
authors.affiliation: Laboratoire de Recherche en Inflammation et Physiologie des Granulocytes, Université du Québec, Canada.
authors.forename: Claudie
authors.initials: C
authors.lastname: Noël
authors.affiliation: Axe Maladies Infectieuses et Immunitaires, Centre de Recherche du CHU de Québec, and Faculté de Médecine, Université Laval, Québec, Canada.
authors.forename: Philippe A
authors.initials: PA
authors.lastname: Tessier
authors.affiliation: Laboratoire de Recherche en Inflammation et Physiologie des Granulocytes, Université du Québec, Canada. Electronic address: Denis.girard@iaf.inrs.ca.
authors.forename: Denis
authors.initials: D
authors.lastname: Girard
mesh_terms: D040501:Calgranulin A / Q000378:metabolism; D040502:Calgranulin B / Q000378:metabolism*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D007113:Immunity, Innate / Q000187:drug effects; D018836:Inflammation Mediators / Q000378:metabolism; D016209:Interleukin-8 / Q000096:biosynthesis*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D016328:NF-kappa B / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D050799:STAT5 Transcription Factor / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C115163:CREB1 protein, human; C508606:CXCL8 protein, human; D040501:Calgranulin A; D040502:Calgranulin B; D017362:Cyclic AMP Response Element-Binding Protein; D018836:Inflammation Mediators; D016209:Interleukin-8; D016328:NF-kappa B; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D050799:STAT5 Transcription Factor; D014157:Transcription Factors; D009240:N-Formylmethionine Leucyl-Phenylalanine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D051550:I-kappa B Kinase
keywords: CREB-1; NF-κB; Neutrophil; S100A9; STAT-3; STAT-5