title: Metaxins 1 and 2, two proteins of the mitochondrial protein sorting and assembly machinery, are essential for Bak activation during TNF alpha triggered apoptosis.
abstract: The proteins Bax and Bak are central in the execution phase of apoptosis; however, little is known about the partners involved in the control of this complex process. Here, we show that mitochondrial Bak is incorporated into a VDAC2/Mtx1/Mtx2 multi-protein complex in both resting and dying cells. VDAC2 is a porin that has previously been described as a partner of Bak while Mtx1 and Mtx2 are two proteins of the mitochondrial sorting and assembly machinery (SAM) that have been implicated in TNF-induced apoptosis. We show that, after the induction of apoptosis, Bak switches from its association with Mtx2 and VDAC2 to interact with Mtx1.
authors.affiliation: Team 9 (Equipe labellisée Ligue Nationale contre le Cancer), Centre de Recherche en Cancérologie Nantes-Angers, UMR INSERM 892/CNRS UMR 6299, F-44007 Nantes, France; Université de Nantes, Faculté de Médecine, 9 Quai Moncousu, 44035 Nantes Cedex 01, France.
authors.forename: Pierre-François
authors.initials: PF
authors.lastname: Cartron
authors.affiliation: Team 9 (Equipe labellisée Ligue Nationale contre le Cancer), Centre de Recherche en Cancérologie Nantes-Angers, UMR INSERM 892/CNRS UMR 6299, F-44007 Nantes, France; Université de Nantes, Faculté de Médecine, 9 Quai Moncousu, 44035 Nantes Cedex 01, France.
authors.forename: Elise
authors.initials: E
authors.lastname: Petit
authors.affiliation: Team 9 (Equipe labellisée Ligue Nationale contre le Cancer), Centre de Recherche en Cancérologie Nantes-Angers, UMR INSERM 892/CNRS UMR 6299, F-44007 Nantes, France; Université de Nantes, Faculté de Médecine, 9 Quai Moncousu, 44035 Nantes Cedex 01, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Bellot
authors.affiliation: Team 9 (Equipe labellisée Ligue Nationale contre le Cancer), Centre de Recherche en Cancérologie Nantes-Angers, UMR INSERM 892/CNRS UMR 6299, F-44007 Nantes, France; Université de Nantes, Faculté de Médecine, 9 Quai Moncousu, 44035 Nantes Cedex 01, France; CHU de Nantes, 1 place Alexis-Ricordeau, 44093 Nantes Cedex 1, France.
authors.forename: Lisa
authors.initials: L
authors.lastname: Oliver
authors.affiliation: Team 9 (Equipe labellisée Ligue Nationale contre le Cancer), Centre de Recherche en Cancérologie Nantes-Angers, UMR INSERM 892/CNRS UMR 6299, F-44007 Nantes, France; Université de Nantes, Faculté de Médecine, 9 Quai Moncousu, 44035 Nantes Cedex 01, France; Institut de Cancérologie de l'Ouest, Centre René Gauducheau, Saint Herblain, France. Electronic address: francois.vallette@inserm.fr.
authors.forename: François M
authors.initials: FM
authors.lastname: Vallette
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D003513:Cycloheximide / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D011506:Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D050996:Voltage-Dependent Anion Channel 2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: C093550:MTX1 protein, human; C522813:MTX2 protein, human; D008565:Membrane Proteins; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha; C494765:VDAC2 protein, human; D050996:Voltage-Dependent Anion Channel 2; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051028:bcl-2-Associated X Protein; D003513:Cycloheximide
keywords: Apoptosis; Bak; Glioma; Metaxins; Mitochondria; Protein import