title: Prevalence and risk factors of Giardia doudenalis in dogs from China.
abstract: The aim of this study was to carry out a survey for the presence of Giardia duodenalis infection in canine using ELISA and PCR and to identify risk factors for infection. Samples from 318 dogs' feces living in nine cities in China were used in the present study. Each sample was tested for the presence of G. duodenalis-specific antigens using ELISA and 197 out of 318 samples were further examined for the presence of G. duodenalis using PCR. The overall rate of canines infected with giardiasis in the present study was 16.04% and 15.22% using ELISA and PCR, respectively. No significant difference was found between sex and Giardia positivity. Young dogs (up to one year) and living in communities were identified as risk factors for infection by multivariate logistic regression analysis. In conclusion, giardiasis in dogs was present in nine cities in China; as risk factors, young dogs (up to one year) and living in communities were of great significance. Giardia-infected canine should be treated for hygienic management to prevent transmission of the infection from dog to human.
authors.affiliation: a College of Animal Science & Veterinary Medicine , Shandong Agricultural University , Taian , P.R. China.
authors.forename: Dubao
authors.initials: D
authors.lastname: Yang
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhang
authors.forename: Limei
authors.initials: L
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Dong
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Jing
authors.forename: Zhan
authors.initials: Z
authors.lastname: Li
authors.forename: Jianzhu
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000469:parasitology; D005260:Female; D005872:Giardia / Q000302:isolation & purification*; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012307:Risk Factors
keywords: China; ELISA; Giardia; PCR; dog; risk factors