title: 4-Amino-1-(3-mercapto-propyl)-pyridine hexafluorophosphate ionic liquid functionalized gold nanoparticles for IgG immunosensing enhancement.
abstract: A novel ionic liquid, 4-amino-1-(3-mercapto-propyl)-pyridine hexafluorophosphate (AMPPH), was successfully synthesized and characterized. Subsequently, AMPPH was used as a functional monomer to fabricate AMPPH-modified gold nanoparticles (AMPPH-AuNPs) via a one-pot synthesis method. The as-prepared AMPPH-AuNPs were confirmed with transmission electron microscopy and X-ray photoelectron spectroscopy. AMPPH-AuNPs were used to construct a biocompatible interface to immobilize rabbit anti-human IgG (anti-HIgG) onto a glassy carbon electrode (GCE) surface, followed by a cross-linking step with glutaraldehyde to fabricate an anti-HIgG-AMPPH-AuNPs/GCE. The nonspecific binding sites were enclosed with bovine serum albumin (BSA) to develop an immunosensor for human IgG. Electrochemical impedance spectroscopy, cyclic voltammetry and differential pulse voltammetry were used to investigate the electrochemical properties of the developed immunosensor. The results indicate that AMPPH-AuNPs can improve the immunosensing performance. The current response of the immunosensor was found linearly related to human IgG concentration in the range of 0.1-5.0 ng mL(-1) and 5.0-100.0 ng mL(-1). The detection limit is estimated to be 0.08 ng mL(-1) (S/N = 3). The obtained immunosensor was successfully applied to the analysis human IgG immunoglobulin in human serum, and the results were well consistent with ELISA method.
authors.affiliation: Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities , Wuhan 430074, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Kangbing
authors.initials: K
authors.lastname: Wu
authors.forename: Changxian
authors.initials: C
authors.lastname: Liu
authors.forename: Yin
authors.initials: Y
authors.lastname: Huang
authors.forename: Yanying
authors.initials: Y
authors.lastname: Wang
authors.forename: Huaifang
authors.initials: H
authors.lastname: Fang
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Chunya
authors.initials: C
authors.lastname: Li
mesh_terms: D000631:Aminopyridines / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007120:Immunochemistry / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis*; D052578:Ionic Liquids / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D056951:Photoelectron Spectroscopy; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D000631:Aminopyridines; D007074:Immunoglobulin G; D052578:Ionic Liquids; D012710:Serum Albumin, Bovine; D006046:Gold