title: Bradykinin and histamine-induced cytosolic calcium increase in capillary endothelial cells of bovine adrenal medulla.
abstract: We have assessed the effect of bradykinin and histamine on the cytosolic free calcium concentration ([Ca(2+)]i ) of bovine adrenal medulla capillary endothelial cells (BAMCECs). To measure [Ca(2+)]i changes in BAMCECs the intracellular fluorescent probe, fluo-3 AM, was used. Bradykinin (3 µM) produced a transient monophasic increase in [Ca(2+)]i , which was depressed by B1650 (0.1 µM), a B2-bradykinin receptor antagonist (D-Arg-[Hyp(3), Thi(5,8) , D-Phe(7)]-Bradykinin). Similarly, increase in [Ca(2+)]i induced by histamine was also depressed by tripolidine (0.1 µM), an H1-histamine receptor antagonist. [Ca(2+)]i increase induced by both agonists was unaffected in the absence of extracellular Ca(2+) or presence of antagonists of voltage operated Ca(2+) channels (VOCCs). Thapsigargin (1 µM) did not abolish the increase of [Ca(2+)]i produced by bradykinin, but abolished that of histamine. In contrast, caffeine (100 µM), abolished the [Ca(2+)]i response induced by bradykinin (3 µM), but did not affect the [Ca(2+)]i increase induced by histamine (100 µM). The results indicate the presence of B2 bradykinin- and H1 histamine-receptors in BAMCECs. Liberation of Ca(2+) induced by both agonists occurs through 2 different intracellular mechanisms. While bradykinin activates a sarco(endo) plasmic reticulum (SER) containing a SER Ca(2+) -ATPase (SERCA) thapsigargin-insensitive, histamine activates a SER containing a SERCA thapsigargin-sensitive. We suggest that the increase in [Ca(2+)]i induced by bradykinin and histamine could be of physiological relevance, modulating adrenal gland microcirculation.
authors.affiliation: Laboratory of Pharmacology, Faculty of Pharmacy, Universidad de Valparaíso, Gran Bretaña 1093, 2360102 Valparaíso, Chile; Centro Regional de Estudios en Alimentos Saludables (CREAS), Región de Valparaíso, Avda. Universidad 330, 2360102 Valparaíso, Chile.
authors.forename: Raúl
authors.initials: R
authors.lastname: Vinet
authors.forename: Magdalena P
authors.initials: MP
authors.lastname: Cortés
authors.forename: Rocío
authors.initials: R
authors.lastname: Alvarez
authors.forename: Marco A
authors.initials: MA
authors.lastname: Delpiano
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology*; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology*; D011969:Receptors, Histamine H1 / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; D011969:Receptors, Histamine H1; D002110:Caffeine; D019284:Thapsigargin; D006632:Histamine; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D001920:Bradykinin; D002118:Calcium
keywords: adrenal gland; bradykinin; capillary endothelial cells; catecholamine; chromaffin cells; histamine