title: Double-strand break repair and genetic recombination in topoisomerase and primase mutants of bacteriophage T4.
abstract: The effects of primase and topoisomerase II deficiency on the double-strand break (DSB) repair and genetic recombination in bacteriophage T4 were studied in vivo using focused recombination. Site-specific DSBs were induced by SegC endonuclease in the rIIB gene of one of the parents. The frequency/distance relationship was determined in crosses of the wild-type phage, topoisomerase II mutant amN116 (gene 39), and primase mutant E219 (gene 61). Ordinary two-factor (i×j) and three-factor (i k×j) crosses between point rII mutations were also performed. These data provide information about the frequency and distance distribution of the single-exchange (splice) and double-exchange (patch) events. In two-factor crosses ets1×i, the topoisomerase and primase mutants had similar recombinant frequencies in crosses at ets1-i distances longer than 1000 bp, comprising about 80% of the corresponding wild-type values. They, however, differ remarkably in crosses at shorter distances. In the primase mutant, the recombinant frequencies are similar to those in the wild-type crosses at distances less than 100 bp, being a bit diminished at longer distances. In two-factor crosses ets1×i of the topoisomerase mutant, the recombinant frequencies were reduced ten-fold at the shortest distances. In three-factor crosses a6 ets1×i, where we measure patch-related recombination, the primase mutant was quite proficient across the entire range of distances. The topoisomerase mutant crosses demonstrated virtually complete absence of rII(+) recombinants at distances up to 33 bp, with the frequencies increasing steadily at longer distances. The data were interpreted as follows. The primase mutant is fully recombination-proficient. An obvious difference from the wild-type state is some shortage of EndoVII function leading to prolonged existence of HJs and thus stretched out ds-branch migration. This is also true for the topoisomerase mutant. However, the latter is deficient in the ss-branch migration step of the DSB repair pathway and partially deficient in HJ initiation. In apparent contradiction to their effects on the DSB-induced site-specific recombination, the topoisomerase and primase mutants demonstrated about 3-8-fold increase in the recombinant frequencies in the ordinary crosses, with the recombination running exclusively via patches. This implies that most of the spontaneous recombination events are not initiated by dsDNA ends in these mutants.
authors.affiliation: Institute of Problems of Chemical Physics RAS, Chernogolovka, Moscow Region 142432, Russia. Electronic address: svp@icp.ac.ru.
authors.forename: Victor P
authors.initials: VP
authors.lastname: Shcherbakov
authors.affiliation: Institute of Problems of Chemical Physics RAS, Chernogolovka, Moscow Region 142432, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Kudryashova
mesh_terms: D017122:Bacteriophage T4 / Q000201:enzymology* / Q000235:genetics; D019915:DNA Primase / Q000235:genetics / Q000378:metabolism*; D026942:DNA Topoisomerases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011995:Recombination, Genetic; D059767:Recombinational DNA Repair; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D019915:DNA Primase; D026942:DNA Topoisomerases
keywords: Bacteriophage T4; Double-strand break repair; Gene 39; Gene 61; Genetic recombination; Primase; RDR; Recombinational analysis; Topoisomerase II