title: Generation of cellular micropatterns on a single-layered graphene film.
abstract: Micropatterns of fibroblast and hippocampal neurons are generated on a single-layered graphene substrate. A large-area (1 cm × 1 cm) graphene film on Si/SiO2 is functionalized by surface-initiated ATRP of non-biofouling oligo(ethylene glycol) methacrylate, after grafting of the polymerization initiator bearing α-bromoisobutylate via photoreaction of the perfluorophenyl azide group. The microcontact printing-assisted spatio-selective reaction, after chemical activation of the terminal hydroxyl group of oligo(ethylene glycol) in the polymeric film, is utilized to generate the patterns of fibroblast and hippocampal neurons.
authors.affiliation: Molecular-Level Interface Research Center, Department of Chemistry, KAIST, Daejeon, 305-701, Korea.
authors.forename: Daewha
authors.initials: D
authors.lastname: Hong
authors.forename: KiEun
authors.initials: K
authors.lastname: Bae
authors.forename: Sangjin
authors.initials: S
authors.lastname: Yoo
authors.forename: Kyungtae
authors.initials: K
authors.lastname: Kang
authors.forename: Byoungwook
authors.initials: B
authors.lastname: Jang
authors.forename: Jinkyu
authors.initials: J
authors.lastname: Kim
authors.forename: Sehun
authors.initials: S
authors.lastname: Kim
authors.forename: Seokwoo
authors.initials: S
authors.lastname: Jeon
authors.forename: Yoonkey
authors.initials: Y
authors.lastname: Nam
authors.forename: Yang-Gyun
authors.initials: YG
authors.lastname: Kim
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D006108:Graphite / Q000737:chemistry*; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D009474:Neurons / Q000166:cytology*; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization; D061251:Primary Cell Culture; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D006108:Graphite
keywords: aryl azide; cells; graphene; micropatterns; surface-initiated polymerization