title: Lack of association of 1513 A/C polymorphism in P2X7 gene with susceptibility to pulmonary and extrapulmonary tuberculosis.
abstract: BACKGROUND
  Tuberculosis, is one of the a leading causes of death worldwide, is characterized by different clinical forms including: latent, localized pulmonary infection and extrapulmonary tuberculosis. Candidate gene association studies have implicated common polymophisms in genes that may influence the development of tuberculosis. This study, aimed to elucidate the role of P2X7 gene in 1513A/C polymorphism the etiopathogenesis of tuberculosis.
  
  
  METHODS
  The study included 160 patients with tuberculosis (71 pulmonary and 89 extrapulmonary tuberculosis) and 160 healthy controls. Genomic DNA was isolated and 1513A/C polymorphism in P2X(7) gene was genotyped by PCR-RFLP method.
  
  
  RESULTS
  Frequency of P2X7 AA genotype was 47.5% in controls and 56.87% in patients, AC frequency was 39.37% controls and 32.5% in patients, CC genotype was 13.12% in controls and 10.62% in patients. No significant difference in allele and genotype frequencies (1513A/C polymorphism) between tuberculosis patients and controls was found.
  
  
  CONCLUSIONS
  The results suggest that 1513A/C polymorphism of P2X7 gene is not associated with pulmonary and extrapulmonary tuberculosis in the Eastern Turkey.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Bartin University, Bartin, Turkey. ozdemirfethiahmet23@yahoo.com.
authors.forename: Fethi Ahmet
authors.initials: FA
authors.lastname: Ozdemir
authors.forename: Deniz
authors.initials: D
authors.lastname: Erol
authors.forename: Vahit
authors.initials: V
authors.lastname: Konar
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Yüce
authors.forename: Ebru
authors.initials: E
authors.lastname: Kara Şenli
authors.forename: Funda
authors.initials: F
authors.lastname: Bulut
authors.forename: Figen
authors.initials: F
authors.lastname: Deveci
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D058486:Receptors, Purinergic P2X7 / Q000235:genetics*; D014376:Tuberculosis / Q000235:genetics*; D014397:Tuberculosis, Pulmonary / Q000235:genetics
chemical_list: D058486:Receptors, Purinergic P2X7