title: Effect of trehalose on Lateolabrax japonicus myofibrillar protein during frozen storage.
abstract: The effect of trehalose on the denaturation of weever (Lateolabrax japonicus) myofibrillar protein during frozen storage at -18 °C for 90 d was investigated. Trehalose (2.5-10 g dry weight) was added to 100 g of myofibrillar protein, and changes in the Ca(2+)-adenylpyrophosphatase (ATPase) activity, solubility, sulfhydryl content, and unfrozen water content of myofibrils were examined during frozen storage. Ca(2+)-ATPase activity and myofibrillar protein solubility decreased gradually during frozen storage at -18 °C upon addition of trehalose. By contrast, Ca(2+)-ATPase activity and myofibrillar protein solubility in the control group dropped drastically during the first 45 d of storage and then further decreased gradually for up to 90 d of storage, indicating a biphasic denaturation pattern. Trehalose addition significantly increased sulfhydryl and unfrozen water contents in the myofibrillar protein of the treatment groups compared with that of the control group (p<0.05) during frozen storage at -18 °C.
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China. Electronic address: wushengjun008@sina.com.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wu
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China.
authors.forename: Saikun
authors.initials: S
authors.lastname: Pan
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D005399:Fishes; D061353:Food Storage; D005615:Freezing; D014199:Trehalose / Q000737:chemistry*
chemical_list: D014199:Trehalose
keywords: Ca(2+)-ATP(ase); Myofibrillar; Solubility; Sulfhydryl content; Trehalose; Unfrozen water