title: [IL-10 level in allergic rats infected by Echinococcus granulosus].
abstract: Forty Wistar rats were randomly divided into 4 groups with 10 in each group. Group A served as normal control. The other 3 groups were injected intraperitoneally with Echinococcus granulosus protoscoleces 5 x 10(4) each. Six months later, group D was injected intraperitoneally with antibody against IL-10 2.0 microg/time, twice a day for 2 d. Two days later, rats in groups C and D were injected intraperitoneally with cyst fluid 5 ml each to induce allergic reaction. 30 min later, all the rats were sacrificed to observe the infection status and obtain peripheral blood. The level of IL-10, IgE and histamine in the sera was detected by ELISA. The results showed that 5, 6 and 5 rats in groups B, C and D were infected successfully, respectively, with 2 deaths in group A. The rats in groups C and D appeared nasal itching, sneezing and declined activity. 30 min later, the symptoms in group C got improved, but not for group D. The levels of IL-10 and IgE in groups C and D increased significantly compared to group B (P < 0.05). The levels of IL-10 and IgE in group D were (142.61 +/- 43.58) pg/ml and (20.67 +/- 1.58) microg/ml, respectively, lower than those in group C (P < 0.05). The level of histamine was (17.69 +/- 3.90) ng/ml, higher than that in groups B and C (P < 0.05). There was no significant difference in histamine level between B and C (P > 0.05).
authors.forename: Ran
authors.initials: R
authors.lastname: An
authors.forename: Mou
authors.initials: M
authors.lastname: Huang
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zheng
mesh_terms: D000707:Anaphylaxis / Q000097:blood* / Q000469:parasitology; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D004443:Echinococcosis / Q000097:blood*; D048209:Echinococcus granulosus; D005260:Female; D006632:Histamine / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood; D016753:Interleukin-10 / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000909:Antibodies, Helminth; D016753:Interleukin-10; D007073:Immunoglobulin E; D006632:Histamine