title: Differentially-expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis.
abstract: Psoriasis is a T cell-mediated, chronic, relapsing and inflammatory cutaneous disorder. The dysfunctional activity of T cells in patients with psoriasis is attributed to bone marrow hematopoietic stem cells (BMHSCs). To understand the pathogenic roles of BMHSCs in psoriasis, a differential gene expression analysis was performed using suppression subtractive hybridization of the BMHSCs from a patient with psoriasis and a healthy control. Using a cDNA array dot blot screening to screen 600 genes from forward- and reverse-subtracted cDNA libraries, 17 differentially-expressed sequence tags (ESTs) were identified. The genes within the ESTs were observed to be the homologs of genes that are involved in various cellular processes, including hormone signaling, RNA catabolism, protein ADP DNA base melting, transcriptional regulation, cell cycle regulation and metabolism. CD45, which was overexpressed in the psoriatic BMHSCs, was further analyzed using relative quantitative polymerase chain reaction. In addition, the levels of CD45 in the peripheral blood cells (PBCs) of the patients with psoriasis were markedly increased and closely associated with disease severity. An abnormality of hematopoietic progenitor cells, e.g., CD45 overexpression, may be transferred to PBCs via hematopoiesis, and may account for the psoriasis-inducing properties of activated T cells.
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Dalian Dermatosis Hospital, Dalian, Liaoning, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Pan
authors.initials: P
authors.lastname: Tian
authors.affiliation: Department of Dermatology, Hong Kong University-Shenzhen Hospital, Shenzhen, Guangdong 518053, P.R. China.
authors.forename: Suchun
authors.initials: S
authors.lastname: Hou
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Shixin
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Tan
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Piao
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liu
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D020224:Expressed Sequence Tags; D005260:Female; D015723:Gene Library; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D012720:Severity of Illness Index; D066251:Subtractive Hybridization Techniques
chemical_list: D012333:RNA, Messenger; D017493:Leukocyte Common Antigens