title: Soluble adhesion molecules as markers of native arteriovenous fistula thrombosis in children on uremia.
abstract: Vascular access represents a lifeline for children undergoing hemodialysis. A failure of vascular access among patients receiving regular hemodialysis is associated with increased morbidity, mortality and costs. We assessed the possibility of using soluble adhesion molecules as reliable predictors of vascular access failure in children on hemodialysis. Moreover, we evaluated whether there is an association among the different studied adhesion molecules in hemodialysis patients with thrombosed and non-thrombosed arteriovenous fistula fistulas (AVFs). This study included 55 hemodialysis children, 36 with good access and 19 with access failure, and 20 healthy volunteers. Forty-four patients had native AVFs and 11 patients had tunneled permanent catheter (11with thrombosed and 33 with non-thrombosed AVFs). Serum-soluble vascular cell adhesion molecule-1 (sVCAM-1), soluble intercellular adhesion molecule-1 (sICAM-1), soluble E-selectin (sE-selectin) and soluble P-selectin (sP-selectin) were measured using ELISA technique. A significant increase was found in the levels of sVCAM-1, sICAM-1, sE-selectin and sP-selectin versus controls and all hemodialysis patients, hemodialysis patients with good access and hemodialysis patients with access failure (P=0.001 for sVCAM-1 and sICAM-1 and P=0.0001 for sE-selectin and sP-selectin). A significant increase was found in the levels of sVCAM-1, sE-selectin and sP-selectin in both chronic hemodialysis patients with thrombosed and non-thrombosed native AVFs versus controls (P=0.0001 for all parameters). There was significant difference between both chronic hemodialysis patients with thrombosed and non-thrombosed native AVFs as regard to sVCAM-1 (54.64±30.82 versus 25.69±27.96ng/ml, P=0.04). Both sICAM-1 and sP-selectin were positively correlated with the erythropoietin (EPO) dose in hemodialysis children (r=0.31, P=0.04 and r=0.32, P=0.04, respectively). A significant positive association was found between E-selectin and sP-selectin in hemodialysis patients with thrombosed AVFs (r=0.83, P=0.04). There was a significant correlation between sVCAM-1 and EPO dose in thrombosed AVF group (r=0.84, P=0.01). The assessment of serum sVCAM-1 might be useful for the identification of the chronic hemodialysis patients at an increased risk for native AVFs thrombosis. The role of EPO in vascular access failure should be taken into consideration. The clinical relevance of these observations warrants further investigations.
authors.affiliation: aDepartment of Pediatrics, Faculty of Medicine, Cairo University bDepartment of Pediatrics, National Research Centre, Cairo, Egypt cAcademic renal unit, Bristol University, Bristol, UK dDepartment of Clinical & Chemical Pathology, National Research Centre eDepartment of Pediatrics, Faculty of Medicine, Taibah University, Al- Madinah Al- Munawwarah, Kingdom of Saudi Arabia and Child Health Department, National Research Centre, Cairo, Egypt.
authors.forename: Fatina I
authors.initials: FI
authors.lastname: Fadel
authors.forename: Manal F
authors.initials: MF
authors.lastname: Elshamaa
authors.forename: Marwa M
authors.initials: MM
authors.lastname: Nabhan
authors.forename: Rascha G
authors.initials: RG
authors.lastname: Essam
authors.forename: Nagwa
authors.initials: N
authors.lastname: Kantoush
authors.forename: Marwa M
authors.initials: MM
authors.lastname: El Sonbaty
authors.forename: Mona
authors.initials: M
authors.lastname: Raafat
authors.forename: Dalia A
authors.initials: DA
authors.lastname: Abd-El Haleem
mesh_terms: D001164:Arteriovenous Fistula / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D015815:Cell Adhesion Molecules / Q000097:blood*; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D013927:Thrombosis / Q000097:blood*; D014511:Uremia / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D015815:Cell Adhesion Molecules