title: Atypical stress response to temperature and NaOCl exposure leading to septation defect during cell division in Cupriavidus metallidurans CH34.
abstract: Cupriavidus metallidurans CH34 has long been known for its temperature-induced mutagenesis and mortality phenotype (TIMM), for which a genetic origin has been suggested repeatedly. In this report, we present microscopic-based evidences that the TIMM process actually starts with a septation defect, leading to aberrant cell morphologies. Moreover, the septation defect of CH34 could be induced by NaOCl, thus showing that the TIMM phenotype may be part of a more general stress response. Sequence analysis of a TIMM survivor exhibiting a recurrent recognizable lysA mutation ruled out the possibility of a genetic ground linking TIMM survival and peptidoglycan synthesis.
authors.forename: Boussad
authors.initials: B
authors.lastname: Arroua
authors.forename: Xavier
authors.initials: X
authors.lastname: Bellanger
authors.forename: Hélène
authors.initials: H
authors.lastname: Guilloteau
authors.forename: Laurence
authors.initials: L
authors.lastname: Mathieu
authors.forename: Christophe
authors.initials: C
authors.lastname: Merlin
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects*; D049921:Cupriavidus / Q000166:cytology* / Q000187:drug effects* / Q000235:genetics; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D012973:Sodium Hypochlorite; D002262:Carboxy-Lyases; C079912:LysA protein, Bacteria