title: Biological and metabolic response in STS-135 space-flown mouse skin.
abstract: There is evidence that space flight condition-induced biological damage is associated with increased oxidative stress and extracellular matrix (ECM) remodeling. To explore possible mechanisms, changes in gene expression profiles implicated in oxidative stress and in ECM remodeling in mouse skin were examined after space flight. The metabolic effects of space flight in skin tissues were also characterized. Space Shuttle Atlantis (STS-135) was launched at the Kennedy Space Center on a 13-day mission. Female C57BL/6 mice were flown in the STS-135 using animal enclosure modules (AEMs). Within 3-5 h after landing, the mice were euthanized and skin samples were harvested for gene array analysis and metabolic biochemical assays. Many genes responsible for regulating production and metabolism of reactive oxygen species (ROS) were significantly (p < 0.05) altered in the flight group, with fold changes >1.5 compared to AEM control. For ECM profile, several genes encoding matrix and metalloproteinases involved in ECM remodeling were significantly up-/down-regulated following space flight. To characterize the metabolic effects of space flight, global biochemical profiles were evaluated. Of 332 named biochemicals, 19 differed significantly (p < 0.05) between space flight skin samples and AEM ground controls, with 12 up-regulated and 7 down-regulated including altered amino acid, carbohydrate metabolism, cell signaling, and transmethylation pathways. Collectively, the data demonstrated that space flight condition leads to a shift in biological and metabolic homeostasis as the consequence of increased regulation in cellular antioxidants, ROS production, and tissue remodeling. This indicates that astronauts may be at increased risk for pathophysiologic damage or carcinogenesis in cutaneous tissue.
authors.affiliation: Division of Radiation Research, Department of Basic Sciences, Loma Linda University and Medical Center , Loma Linda, CA , USA.
authors.forename: X W
authors.initials: XW
authors.lastname: Mao
authors.forename: M J
authors.initials: MJ
authors.lastname: Pecaut
authors.forename: L S
authors.initials: LS
authors.lastname: Stodieck
authors.forename: V L
authors.initials: VL
authors.lastname: Ferguson
authors.forename: T A
authors.initials: TA
authors.lastname: Bateman
authors.forename: M L
authors.initials: ML
authors.lastname: Bouxsein
authors.forename: D S
authors.initials: DS
authors.lastname: Gridley
mesh_terms: D000818:Animals; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D055432:Metabolomics; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000502:physiology; D012867:Skin / Q000378:metabolism* / Q000473:pathology*; D013026:Space Flight
keywords: STS-135; metabolomic profiling; oxidative stress; skin; space flight