title: Biophysical characterization of genistein-membrane interaction and its correlation with biological effect on cells - The case of EYPC liposomes and human erythrocyte membranes.
abstract: With application of EPR and (1)H NMR techniques genistein interaction with liposomes formed with egg yolk lecithin and with erythrocyte membranes was assessed. The present study addressed the problem of genistein localization and its effects on lipid membrane fluidity and protein conformation. The range of microscopic techniques was employed to study genistein effects on HeLa cells and human erythrocytes. Moreover, DPPH bioassay, superoxide anion radical test and enzymatic measurements were performed in HeLa cells subjected to genistein. The gathered results from both EPR and NMR techniques indicated strong ordering effect of genistein on the motional freedom of lipids in the head group region and the adjacent hydrophobic zone in liposomal as well as in red blood cell membranes. EPR study of human ghost showed also the changes in the erythrocyte membrane protein conformation. The membrane effects of genistein were correlated with the changes in internal membranes arrangement of HeLa cells as it was noticed using transmission electron microscopic and fluorescent techniques. Scanning electron and light microscopy methods showed that one of the aftermaths of genistein incorporation into membranes was creation of echinocytic form of the red blood cells with reduced diameter. Genistein improved redox status of HeLa cells treated with H2O2 by lowering radicals' level. In conclusion, the capacity of genistein to incorporate, to affect membrane organization and to change its biophysical properties is correlated with the changes inside the cells.
authors.affiliation: Department of Comparative Anatomy and Anthropology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland; Electron Microscopy Laboratory, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: bozka1996@o2.pl.
authors.forename: Bożena
authors.initials: B
authors.lastname: Pawlikowska-Pawlęga
authors.affiliation: Institute of Physics, Maria Curie-Skłodowska University, Pl. M. Curie-Skłodowskiej 1, 20-031 Lublin, Poland. Electronic address: lucjan.misiak@umcs.pl.
authors.forename: Lucjan E
authors.initials: LE
authors.lastname: Misiak
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: anna.wilkolazka@poczta.umcs.lublin.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Jarosz-Wilkołazka
authors.affiliation: Department of Cell Biology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: basiaza@vp.pl.
authors.forename: Barbara
authors.initials: B
authors.lastname: Zarzyka
authors.affiliation: Department of Virology and Immunology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: rpaduch@poczta.umcs.lublin.pl.
authors.forename: Roman
authors.initials: R
authors.lastname: Paduch
authors.affiliation: Department of Comparative Anatomy and Anthropology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: agawron@poczta.umcs.lublin.pl.
authors.forename: Antoni
authors.initials: A
authors.lastname: Gawron
authors.affiliation: Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University, Pl. M. Curie-Skłodowskiej 1, 20-031 Lublin, Poland. Electronic address: wieslaw.gruszecki@umcs.pl.
authors.forename: Wieslaw I
authors.initials: WI
authors.lastname: Gruszecki
mesh_terms: D016588:Anticarcinogenic Agents / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism*; D019833:Genistein / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence
chemical_list: D016588:Anticarcinogenic Agents; D000975:Antioxidants; D005609:Free Radicals; D008051:Lipid Bilayers; D008081:Liposomes; D019833:Genistein
keywords: EPR; EYPC liposomes; Electron microscopy; Genistein; Human ghosts; NMR