title: Recruitment of fat cell precursors during high fat diet in C57BL/6J mice is fat depot specific.
abstract: OBJECTIVE
  The study aims to explore long-term dietary effects on increases in body mass and fat depot enlargement through the recruitment of early in life labeled progenitor cells to the adipolineage.
  
  
  METHODS
  Neonate male C57BL/6J (B6) mice were injected intraperitoneally with BrdU. From 4 until 30 weeks of age they were fed either low fat diet (LFD) or high fat diet (HFD). BrdU-labeled cells were analyzed by flow cytometric and immunohistochemical assays after 10 days and 4, 8, 16, and 30 weeks.
  
  
  RESULTS
  Mice fed HFD were heavier than mice fed LFD with the most dramatic disparity recorded between week 16 and 30. BrdU-bearing cells showed the decrease in the percentage content of labeled cells in inguinal (iWAT), epididymal (eWAT) and bone marrow (BM) tissues, regardless diets. However, iWAT collected from animals on HFD showed significant increase in labeled-cells at week 16th, which coincides with robust increase in inguinal but not epididymal fat weight between 16 and 30 weeks age.
  
  
  CONCLUSIONS
  Cells labeled with BrdU during neonate life of B6 mice persist in fat tissues for long period of time and are recruited to the adipocyte lineage in a favorable (obesogenic) environment in iWAT but not in eWAT.
authors.affiliation: Department of Biological Function of Food Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland. b.kozak@pan.olsztyn.pl
authors.forename: B
authors.initials: B
authors.lastname: Gawronska-Kozak
authors.forename: J
authors.initials: J
authors.lastname: Staszkiewicz
authors.forename: J M
authors.initials: JM
authors.lastname: Gimble
authors.forename: H
authors.initials: H
authors.lastname: Kirk-Ballard
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000473:pathology*; D052436:Adipose Tissue, White / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D001823:Body Composition; D050218:Body Fat Distribution; D015992:Body Mass Index; D001853:Bone Marrow / Q000473:pathology; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D018752:Diet, Fat-Restricted; D059305:Diet, High-Fat; D004822:Epididymis / Q000473:pathology; D007264:Inguinal Canal / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
chemical_list: D001973:Bromodeoxyuridine