title: [Over-expression of human SPRY2 promotes the proliferation and survival of HEK293T cells].
abstract: OBJECTIVE
  To construct a recombinant expression vector pDC315/hSPRY2 carrying human SPRY2 (hSPRY2) gene, and transfect the recombinant plasmid into human embryonic kidney HEK293T cells to express the target protein, and thus explore preliminarily the effect of hSPRY2 on the proliferation and survival of HEK293T cells.
  
  
  METHODS
  The recombinant adenoviral vector expressing hSPRY2 was constructed and transfected into HEK293T cells in vitro. The expression of hSPRY2 target protein in the transfected cells was identified by Western blot analysis. The effect of hSPRY2 on the proliferation and survival of HEK293T cells in a serum-free condition was tested using CCK-8 assay.
  
  
  RESULTS
  The recombinant expression vector pDC315/hSPRY2 was constructed, and the target protein hSPRY2 was expressed in the transfected HEK293T cells. The proliferation and survival rates of HEK293T cells transfected with pDC315/hSPRY2 were significantly higher than those transfected with pDC315/EGFP (P<0.05).
  
  
  CONCLUSIONS
  The recombinant expression vector for hSPRY2 was successfully constructed and its expression was demonstrated in the transfected HEK293T cells. Over-expression of SPRY2 promoted the proliferation and survival of HEK293T cells.
authors.affiliation: School of Life Science and Technology, Xinjiang University, Wulumuqi 830046; Department of Translational Medicine, Beijing Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Jingying
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Translational Medicine, Beijing Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: DU
authors.affiliation: Department of Translational Medicine, Beijing Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Translational Medicine, Beijing Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Jiyun
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Translational Medicine, Beijing Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Renli
authors.initials: R
authors.lastname: Tian
authors.affiliation: Department of Translational Medicine, Beijing Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Translational Medicine, Beijing Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D015153:Blotting, Western; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D005434:Flow Cytometry; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C485561:SPRY2 protein, human; D049452:Green Fluorescent Proteins