title: Report: screening of selected medicinal plants for their enzyme inhibitory potential - a validation of their ethnopharmacological uses.
abstract: In present study four medicinal plants namely Valeriana wallichii, Xanthium strumarium, Achyranthes aspera and Duchesnea indica belonging to different families were collected in Khyber Pakhtunkhwa province and crude extract and subsequent fractions were analyzed for their inhibitory potential against acetylcholinesterase, butyrylcholinesterase and α-glucosidase enzymes. Valeriana wallichii, Xanthium strumarium and Achyranthes aspera were significantly active against cholinesterases. Chloroform and ethylacetate fractions of Valeriana wallichii exhibited significant activity against acetylcholinesterase (IC50: 61μg/ml) and butyrylcholinesterase enzymes (IC50: 58μg/ml), respectively. Similarly ethylacetate fraction of Achyranthes aspera showed significant activity against acetylcholinesterase (IC50: 61 μg/ml) and butyrylcholinesterase enzymes (IC50: 61 μg/ml), respectively. In case of α-glucosidase enzyme, the chloroform fraction of Xanthium strumarium exhibited significant inhibitory activity (IC50: 72 μg/ml) as compared to the standard compound acarbose (IC50: 483 μg/ml). Duchesnea indica showed no such activities.
authors.affiliation: Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
authors.forename: Fazli
authors.initials: F
authors.lastname: Khuda
authors.affiliation: Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
authors.forename: Zafar
authors.initials: Z
authors.lastname: Iqbal
authors.affiliation: Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan.
authors.forename: Ayub
authors.initials: A
authors.lastname: Khan
authors.affiliation: Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
authors.lastname: Zakiullah
authors.affiliation: Department of Pharmacy, University of Swabi, Pakistan.
authors.forename: Yasar
authors.initials: Y
authors.lastname: Shah
authors.affiliation: Department of Pharmacy, University of Swabi, Pakistan.
authors.forename: Abad
authors.initials: A
authors.lastname: Khan
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D002091:Butyrylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D020834:Ethnopharmacology; D065089:Glycoside Hydrolase Inhibitors; D010946:Plants, Medicinal
chemical_list: D002800:Cholinesterase Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase