title: Electrochemical detection of Pseudomonas aeruginosa in human fluid samples via pyocyanin.
abstract: The ability to quickly detect the presence of pathogenic bacteria in patient samples is of the outmost importance to expedient patient care. Here we report the direct, selective, and sensitive detection of the opportunistic pathogen Pseudomonas aeruginosa, spiked in human whole blood with sodium heparin, urine, sputum, and bronchial lavage samples using unmodified, disposable carbon electrode sensors that detect the presence of pyocyanin, a virulence factor that is unique to this species. Square wave voltammetry scans of biological fluids from healthy individuals spiked with P. aeruginosa showed a clear pyocyanin response within one day of culturing at 37°C. Scans of supernatants taken from cultures of P. aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, and Bacillus cereus taken over a span of three days in the potential range from -0.5 to 0 V vs. an Ag/AgCl reference showed no electrochemically detectable molecules with the exception of P. aeruginosa. The results indicate the potential to sensitively and selectively determine the presence of P. aeruginosa in human samples via the electrochemical detection of pyocyanin in less than 5 min, without any sample preparation or separation steps.
authors.affiliation: Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, 313 Snell Engineering, Boston, MA 02115, USA.
authors.forename: Thaddaeus A
authors.initials: TA
authors.lastname: Webster
authors.affiliation: Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, 313 Snell Engineering, Boston, MA 02115, USA.
authors.forename: Hunter J
authors.initials: HJ
authors.lastname: Sismaet
authors.affiliation: Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, 313 Snell Engineering, Boston, MA 02115, USA.
authors.forename: Jared L
authors.initials: JL
authors.lastname: Conte
authors.affiliation: Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, 313 Snell Engineering, Boston, MA 02115, USA.
authors.forename: I-ping J
authors.initials: IP
authors.lastname: Chan
authors.affiliation: Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, 313 Snell Engineering, Boston, MA 02115, USA. Electronic address: e.goluch@neu.edu.
authors.forename: Edgar D
authors.initials: ED
authors.lastname: Goluch
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001826:Body Fluids / Q000382:microbiology*; D003217:Conductometry / Q000295:instrumentation*; D004209:Disposable Equipment; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification* / Q000378:metabolism*; D011710:Pyocyanine / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011710:Pyocyanine
keywords: Disposable carbon electrodes; Electrochemical detection; Human biofluids; Pseudomonas aeruginosa; Pyocyanin