title: The canonical Notch signaling was involved in the regulation of intestinal epithelial cells apoptosis after intestinal ischemia/reperfusion injury.
abstract: Notch signaling plays a critical role in the maintenance of intestinal homeostasis. The aim of the present study was to investigate the role of Notch signaling in the apoptosis of intestinal epithelial cells after intestinal ischemia reperfusion (I/R) injury. Male C57BL/6 mice were subjected to sham operation or I/R injury. Intestinal tissue samples were collected at 12 h after reperfusion. TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling) staining showed that intestinal I/R injury induced significantly increased apoptosis of intestinal epithelial cells. Meanwhile, the mRNA expression of Jagged1, DLL1, Notch2, and Hes5, and protein expression of NICD2 and Hes5 were increased significantly after I/R injury in intestinal epithelial cells. In an in vitro IEC-6 culture model, flow cytometry analyses showed that inhibition of Notch signaling by γ-secretase inhibitor DAPT and the suppression of Hes5 expression using siRNA both significantly increased the apoptosis of IEC-6 cells under the condition of hypoxia/ reoxygenation (H/R). In conclusion, the Notch2/Hes5 signaling pathway was activated and involved in the regulation of intestinal epithelial cells apoptosis in intestinal I/R injury.
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. maomaoyu1209@163.com.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. zhicao_zhang@163.com.
authors.forename: Zhicao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. chengyingdong1964@163.com.
authors.forename: Yingdong
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. weidong.xiao@126.com.
authors.forename: Weidong
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. qiuyuan1988@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. yumimianbao@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. slh6260@163.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. happywwsh@163.com.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. guangsheng_du@hotmail.com.
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Du
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. guychao@163.com.
authors.forename: Yingchao
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. pengke620@163.com.
authors.forename: Ke
authors.initials: K
authors.lastname: Peng
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. xuqichao.2007@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. huayang@tmmu.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D051880:Receptors, Notch / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction
chemical_list: D051880:Receptors, Notch