title: Study on the interaction of β-cyclodextrin and berberine hydrochloride and its analytical application.
abstract: The fluorescence enhancement of berberine hydrochloride (BBH) as a result of complex with β-cyclodextrin (β-CD) is investigated. The mechanism of the inclusion was studied and discussed by spectrofluoremetry and infrared spectrograms. The results showed that a 1∶1 (β-CD: BBH) complex was formed with an apparent association constant of 4.23×10(2) L/mol. Based on the enhancement of the fluorescent intensity of berberine hydrochloride, a new spectrofluorimetric method for the determination of BBH in the presence of β-CD was developed. The linear range was 1.00∼4.00 µg/mL with the detection limit of 5.54 ng/mL. The proposed method was successfully applied to the determination of BBH in tablets.
authors.affiliation: College of Pharmaceutical, Taishan Medical University, Tai'an, P.R. China.
authors.forename: Baoxiu
authors.initials: B
authors.lastname: Jia
authors.affiliation: College of Pharmaceutical, Taishan Medical University, Tai'an, P.R. China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Pharmaceutical, Taishan Medical University, Tai'an, P.R. China.
authors.forename: Decai
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Pharmaceutical, Taishan Medical University, Tai'an, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Duan
mesh_terms: D001599:Berberine / Q000494:pharmacology*; D004347:Drug Interactions; D006863:Hydrogen-Ion Concentration; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D047392:beta-Cyclodextrins / Q000494:pharmacology*
chemical_list: D047392:beta-Cyclodextrins; D001599:Berberine; C031215:betadex