title: Impact of short-term liquid storage on human CD133(+) stem cells.
abstract: Stem cell transplantation is a viable strategy for regenerative medicine. However, it is inevitable to have cells undergo storage for several hours or days due to processing and transportation. Therefore, it is crucial to have rigidly controlled conditions ensuring the therapeutic benefit of isolated stem cells. In the present study, we investigated the impact of short-term storage on human CD133(+) cells. CD133(+) cells were isolated from human bone marrow and kept at standardized nonfreezing storage conditions for up to 72 h. Cell viability (apoptosis/necrosis) and expression of CD133 and CXCR4 were analyzed by flow cytometry. Metabolic activity was determined using an MTT assay; colony-forming ability, as well as endothelial-like differentiation, was further evaluated. A qRT-PCR array was employed to investigate the expression of stemness genes. CD133 and CXCR4 expressions were preserved at all time points. After 30 h, cell number and metabolic activity decreased, although no significant changes were detected in cell viability and proliferation as well as endothelial-like differentiation. Cell viability and proliferation decreased significantly only after 72 h of storage. Our results indicate that storage of isolated human CD133(+) bone marrow stem cells in liquid allows for high viability and functionality. However, storage time should be limited in order to avoid cell loss.
authors.affiliation: Reference and Translation Center for Cardiac Stem Cell Therapy, University of Rostock, Rostock, Germany.
authors.forename: Cornelia A
authors.initials: CA
authors.lastname: Lux
authors.forename: Peter
authors.initials: P
authors.lastname: Mark
authors.forename: Christian
authors.initials: C
authors.lastname: Klopsch
authors.forename: Michael
authors.initials: M
authors.lastname: Laupheimer
authors.forename: Hoang
authors.initials: H
authors.lastname: Tu-Rapp
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Li
authors.forename: Nan
authors.initials: N
authors.lastname: Ma
authors.forename: Gustav
authors.initials: G
authors.lastname: Steinhoff
authors.forename: Robert
authors.initials: R
authors.lastname: David
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002470:Cell Survival / Q000502:physiology*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014021:Tissue Preservation
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; C527276:CXCR4 protein, human; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D019718:Receptors, CXCR4