title: Effect of chronic valproic Acid treatment on hepatic gene expression profile in wfs1 knockout mouse.
abstract: Valproic acid (VPA) is a widely used anticonvulsant and mood-stabilizing drug whose use is often associated with drug-induced weight gain. Treatment with VPA has been shown to upregulate Wfs1 expression in vitro. Aim of the present study was to compare the effect of chronic VPA treatment in wild type (WT) and Wfs1 knockout (KO) mice on hepatic gene expression profile. Wild type, Wfs1 heterozygous, and homozygous mice were treated with VPA for three months (300 mg/kg i.p. daily) and gene expression profiles in liver were evaluated using Affymetrix Mouse GeneChip 1.0 ST array. We identified 42 genes affected by Wfs1 genotype, 10 genes regulated by VPA treatment, and 9 genes whose regulation by VPA was dependent on genotype. Among the genes that were regulated differentially by VPA depending on genotype was peroxisome proliferator-activated receptor delta (Ppard), whose expression was upregulated in response to VPA treatment in WT, but not in Wfs1 KO mice. Thus, regulation of Ppard by VPA is dependent on Wfs1 genotype.
authors.affiliation: Institute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, Estonia ; Centre of Excellence for Translational Medicine, 19 Ravila Street, 50411 Tartu, Estonia.
authors.forename: Marite
authors.identifier: 0000-0002-5459-3687
authors.initials: M
authors.lastname: Punapart
authors.affiliation: Institute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, Estonia ; Centre of Excellence for Translational Medicine, 19 Ravila Street, 50411 Tartu, Estonia.
authors.forename: Mall
authors.identifier: 0000-0002-9651-4107
authors.initials: M
authors.lastname: Eltermaa
authors.affiliation: Institute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, Estonia ; Centre of Excellence for Translational Medicine, 19 Ravila Street, 50411 Tartu, Estonia.
authors.forename: Julia
authors.identifier: 0000-0002-5086-8663
authors.initials: J
authors.lastname: Oflijan
authors.affiliation: Institute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, Estonia ; Centre of Excellence for Translational Medicine, 19 Ravila Street, 50411 Tartu, Estonia.
authors.forename: Silva
authors.initials: S
authors.lastname: Sütt
authors.affiliation: Institute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, Estonia ; Centre of Excellence for Translational Medicine, 19 Ravila Street, 50411 Tartu, Estonia.
authors.forename: Anne
authors.identifier: 0000-0002-1235-4442
authors.initials: A
authors.lastname: Must
authors.affiliation: Institute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, Estonia ; Centre of Excellence for Translational Medicine, 19 Ravila Street, 50411 Tartu, Estonia.
authors.forename: Sulev
authors.initials: S
authors.lastname: Kõks
authors.affiliation: SGDP, Institute of Psychiatry at the Maudsley, King's College London, De Crespigny Park, London SE5 8AF, UK.
authors.forename: Leonard C
authors.initials: LC
authors.lastname: Schalkwyk
authors.affiliation: SGDP, Institute of Psychiatry at the Maudsley, King's College London, De Crespigny Park, London SE5 8AF, UK.
authors.forename: Catherine
authors.initials: C
authors.lastname: Fernandes
authors.affiliation: Institute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, Estonia ; Centre of Excellence for Translational Medicine, 19 Ravila Street, 50411 Tartu, Estonia.
authors.forename: Eero
authors.initials: E
authors.lastname: Vasar
authors.affiliation: Centre of Excellence for Translational Medicine, 19 Ravila Street, 50411 Tartu, Estonia ; Institute of Biomedicine and Translational Medicine, Department of Biochemistry, University of Tartu, 19 Ravila Street, 50411 Tartu, Estonia.
authors.forename: Ursel
authors.identifier: 0000-0002-3681-188X
authors.initials: U
authors.lastname: Soomets
authors.affiliation: Institute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, Estonia ; Centre of Excellence for Translational Medicine, 19 Ravila Street, 50411 Tartu, Estonia.
authors.forename: Anton
authors.identifier: 0000-0002-5139-1764
authors.initials: A
authors.lastname: Terasmaa