title: Reversible protein inactivation by optogenetic trapping in cells.
abstract: We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP.
authors.affiliation: 1] Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon, Republic of Korea. [2] Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. [3].
authors.forename: Sangkyu
authors.initials: S
authors.lastname: Lee
authors.affiliation: 1] Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. [2].
authors.forename: Hyerim
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
authors.forename: Taeyoon
authors.initials: T
authors.lastname: Kyung
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
authors.forename: Na Yeon
authors.initials: NY
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
authors.forename: Sungsoo
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
authors.forename: Jihoon
authors.initials: J
authors.lastname: Kim
authors.affiliation: 1] Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon, Republic of Korea. [2] Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
authors.forename: Won Do
authors.initials: WD
authors.lastname: Heo
mesh_terms: D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008027:Light; D061848:Optical Imaging; D062308:Optogenetics; D011506:Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins; D049452:Green Fluorescent Proteins