title: Cyclic adenosine 5'-diphosphoribose (cADPR) cyclic guanosine 3',5'-monophosphate positively function in Ca(2+) elevation in methyl jasmonate-induced stomatal closure, cADPR is required for methyl jasmonate-induced ROS accumulation NO production in guard cells.
abstract: Methyl jasmonate (MeJA) signalling shares several signal components with abscisic acid (ABA) signalling in guard cells. Cyclic adenosine 5'-diphosphoribose (cADPR) and cyclic guanosine 3',5'-monophosphate (cGMP) are second messengers in ABA-induced stomatal closure. In order to clarify involvement of cADPR and cGMP in MeJA-induced stomatal closure in Arabidopsis thaliana (Col-0), we investigated effects of an inhibitor of cADPR synthesis, nicotinamide (NA), and an inhibitor of cGMP synthesis, LY83583 (LY, 6-anilino-5,8-quinolinedione), on MeJA-induced stomatal closure. Treatment with NA and LY inhibited MeJA-induced stomatal closure. NA inhibited MeJA-induced reactive oxygen species (ROS) accumulation and nitric oxide (NO) production in guard cells. NA and LY suppressed transient elevations elicited by MeJA in cytosolic free Ca(2+) concentration ([Ca(2+)]cyt) in guard cells. These results suggest that cADPR and cGMP positively function in [Ca(2+)]cyt elevation in MeJA-induced stomatal closure, are signalling components shared with ABA-induced stomatal closure in Arabidopsis, and that cADPR is required for MeJA-induced ROS accumulation and NO production in Arabidopsis guard cells.
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: M A
authors.initials: MA
authors.lastname: Hossain
authors.forename: W
authors.initials: W
authors.lastname: Ye
authors.forename: S
authors.initials: S
authors.lastname: Munemasa
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: I C
authors.initials: IC
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Murata
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000634:Aminoquinolines; D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D036563:Cyclic ADP-Ribose / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D003517:Cyclopentanes / Q000494:pharmacology*; D009535:Nicotinamidase; D009569:Nitric Oxide / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology*; D018515:Plant Leaves / Q000166:cytology; D054046:Plant Stomata / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000085:Acetates; D000634:Aminoquinolines; D003517:Cyclopentanes; D054883:Oxylipins; D017382:Reactive Oxygen Species; D036563:Cyclic ADP-Ribose; D009569:Nitric Oxide; C072239:methyl jasmonate; C041715:6-anilino-5,8-quinolinedione; D009535:Nicotinamidase; D006152:Cyclic GMP; D002118:Calcium
keywords: Abscisic acid; Arabidopsis thaliana; cyclic adenosine 5′-diphosphoribose; cyclic guanosine 3′,5′-monophosphate; methyl jasmonate