title: Screening on human hepatoma cell line HepG-2 nucleus and cytoplasm protein after CDK2 silencing by RNAi.
abstract: The activation of phase-specific cyclin-dependent kinases is associated with ordered cell cycle transitions. Among the mammalian Cdks, Cdk2 is essential for liver cancer cell proliferation. The related cycling protein CDK2 was analyzed by 2D-gel and MALDI-TOF/TOF MS mass assay in liver cancer cells, which CDK2 was silenced. The results showed four significantly different spots in cell ribonucleoprotein (similar to ribosomal protein S12, chaperonin 10-related protein, beta-actin and zinc finger protein 276) and four in plasmosin (aldolase A protein, hCG, anonymous protein and tubulin, gamma complex associated protein 2). In the plasmosin, aldolase A catalyzes the production of tublin and actin. Together they regulate the cell cycle and arrest the cell in the S phage. In the cell ribonucleoprotein, proteins with homology to ribosomal protein S12 and chaperonin 10 play a similar role in cell cycle regulation.
authors.affiliation: Department of Clinical Laboratory, Inner Mongolia People's Hospital, Hohhot, 010018, People's Republic of China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Han
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.forename: Wenli
authors.initials: W
authors.lastname: Wang
authors.forename: Ruixia
authors.initials: R
authors.lastname: Bai
authors.forename: Pengwei
authors.initials: P
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Shang