title: Influences of pH, urea and metal ions on the interaction of sinomenine with Lysozyme by steady state fluorescence spectroscopy.
abstract: The interaction between sinomenine and Lysozyme (Lys) in aqueous solution has been systemically investigated by fluorescence spectroscopic techniques at pH 7.4. The quenching rate constants and binding constants calculated indicated the static quenching mechanism and medium binding force. The effect of sinomenine on the conformation of Lys was analyzed using synchronous fluorescence and three-dimensional (3D) fluorescence. In addition, influence of pH on the binding of sinomenine to Lys was investigated and the binding ability of the drug to Lys deceased under other pH conditions (pH 9.0, 3.5, and 1.9) as compared with that at pH 7.4. As compared with the binding ability of sinomenine to native Lys, that of sinomenine to denatured Lys deceases dramatically. Furthermore, the effect of many metal ions on the binding constant of sinomenine with Lys was investigated.
authors.affiliation: College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang 471022, China. Electronic address: lyhxxjbm@126.com.
authors.forename: Daojin
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang 471022, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang 471022, China.
authors.forename: Baoming
authors.initials: B
authors.lastname: Ji
mesh_terms: D000818:Animals; D002626:Chemistry, Pharmaceutical; D002645:Chickens; D015195:Drug Design; D004529:Egg White; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008239:Lysine / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D009019:Morphinans / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D014508:Urea / Q000737:chemistry*
chemical_list: D007477:Ions; D008670:Metals; D009019:Morphinans; C009271:sinomenine; D014508:Urea; D009113:Muramidase; D008239:Lysine
keywords: Fluorescence quenching; Lysozyme; Metal ions; Sinomenine; Three-dimensional fluorescence; Urea