title: Study to find the best extraction solvent for use with guava leaves (Psidium guajava L.) for high antioxidant efficacy.
abstract: The effects of guava leaves extracted using solvents of water, ethanol, methanol, and different concentrations of hydroethanolic solvents on phenolic compounds and flavonoids, and antioxidant properties have been investigated. The antioxidant capability was assessed based on 2,2-diphenyl-1-picrylhydrazyl radical and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical-scavenging abilities, reducing power, and nitric oxide-and nitrate-scavenging activities. The results demonstrated that the antioxidant ability of guava leaf extracts has a strong relationship with phenolic compound content rather than flavonoid content. Phenolic compound content of water extracted guava leaves was higher compared to pure ethanol and methanol extracts. However, phenolic compound content extracted using hydroethanolic solvent was higher than water, whereas 50% hydroethanolic was observed to be the most effective solvent showing high antioxidant ability.
authors.affiliation: Department of Nutrition, Food and Exercise Sciences, College of Human Sciences, Florida State University Tallahassee, Florida, 32306.
authors.forename: Jongkwon
authors.initials: J
authors.lastname: Seo
authors.affiliation: Department of Food and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University 501 Jinjudaero, Jinju, 660-701, Korea.
authors.forename: Soojung
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Nutrition, Food and Exercise Sciences, College of Human Sciences, Florida State University Tallahassee, Florida, 32306.
authors.forename: Marcus L
authors.initials: ML
authors.lastname: Elam
authors.affiliation: Department of Nutrition, Food and Exercise Sciences, College of Human Sciences, Florida State University Tallahassee, Florida, 32306.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Johnson
authors.affiliation: Department of Biology, Valdosta State University Valdosta, Georgia, 31698.
authors.forename: Jonghoon
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Nutrition, Food and Exercise Sciences, College of Human Sciences, Florida State University Tallahassee, Florida, 32306.
authors.forename: Bahram H
authors.initials: BH
authors.lastname: Arjmandi
keywords: Antioxidant; flavonoid; guava; hydroethanolic solvent; phenolic compound