title: Two distinct domains of Flo8 activator mediates its role in transcriptional activation and the physical interaction with Mss11.
abstract: Flo8 is a transcriptional activator essential for the inducible expression of a set of target genes such as STA1, FLO11, and FLO1 encoding an extracellular glucoamylase and two cell surface proteins, respectively. However, the molecular mechanism of Flo8-mediated transcriptional activation remains largely elusive. By generating serial deletion constructs, we revealed here that a novel transcriptional activation domain on its extreme C-terminal region plays a crucial role in activating transcription. On the other hand, the N-terminal LisH motif of Flo8 appears to be required for its physical interaction with another transcriptional activator, Mss11, for their cooperative transcriptional regulation of the shared targets. Additionally, GST pull-down experiments uncovered that Flo8 and Mss11 can directly form either a heterodimer or a homodimer capable of binding to DNA, and we also showed that this formed complex of two activators interacts functionally and physically with the Swi/Snf complex. Collectively, our findings provide valuable clues for understanding the molecular mechanism of Flo8-mediated transcriptional control of multiple targets.
authors.affiliation: Department of Medical Science, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Brain Science, DGIST, Daegu 711-873, Republic of Korea.
authors.forename: Sung Bae
authors.initials: SB
authors.lastname: Lee
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Hyen Sam
authors.initials: HS
authors.lastname: Kang
authors.affiliation: Department of Life Science and College of Natural Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Goo Taeg
authors.initials: GT
authors.lastname: Oh
authors.affiliation: Department of Life Science and College of Natural Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea. Electronic address: tskim@ewha.ac.kr.
authors.forename: TaeSoo
authors.initials: T
authors.lastname: Kim
mesh_terms: D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D037241:Mannose-Binding Lectins / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: C087578:FLO1 protein, S cerevisiae; C110690:FLO11 protein, S cerevisiae; C100940:FLO8 protein, S cerevisaie; C109358:MSS11 protein, S cerevisiae; D037241:Mannose-Binding Lectins; D008562:Membrane Glycoproteins; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors
keywords: FLO1; FLO11; Flo8; LisH motif; Mss11; Transcriptional activation