title: Stable accumulation of seed storage proteins containing vaccine peptides in transgenic soybean seeds.
abstract: There has been a significant increase in the use of transgenic plants for the large-scale production of pharmaceuticals and industrial proteins. Here, we report the stable accumulation of seed storage proteins containing disease vaccine peptides in transgenic soybean seeds. To synthesize vaccine peptides in soybean seeds, we used seed storage proteins as a carrier and a soybean breeding line lacking major seed storage proteins as a host. Vaccine peptides were inserted into the flexible disordered regions in the A1aB1b subunit three-dimensional structure. The A1aB1b subunit containing vaccine peptides in the disordered regions were sorted to the protein storage vacuoles where vaccine peptides are partially cleaved by proteases. In contrast, the endoplasmic reticulum (ER)-retention type of the A1aB1b subunit containing vaccine peptides accumulated in compartments that originated from the ER as an intact pro-form. These results indicate that the ER may be an organelle suitable for the stable accumulation of bioactive peptides using seed storage proteins as carriers.
authors.affiliation: Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. Electronic address: marunobu@kais.kyoto-u.ac.jp.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Maruyama
authors.affiliation: Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Fujiwara
authors.affiliation: Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yokoyama
authors.affiliation: Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Cerrone
authors.initials: C
authors.lastname: Cabanos
authors.affiliation: Hokko Chemical Industry Co., Ltd., Atsugi, Kanagawa 243-0023, Japan.
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Hasegawa
authors.affiliation: National Institute of Agrobiological Science, Tsukuba, Ibaraki 305-8602, Japan; National Agricultural Research Center for Hokkaido Region, Sapporo, Hokkaido 062-8555, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takagi
authors.affiliation: National Agricultural Research Center for Hokkaido Region, Sapporo, Hokkaido 062-8555, Japan.
authors.forename: Keito
authors.initials: K
authors.lastname: Nishizawa
authors.affiliation: Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Uki
authors.affiliation: Graduate School of Medicine, Hirosaki University, Hirosaki, Aomori 036-8562, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kawarabayashi
authors.affiliation: Graduate School of Medicine, Hirosaki University, Hirosaki, Aomori 036-8562, Japan.
authors.forename: Mikio
authors.initials: M
authors.lastname: Shouji
authors.affiliation: National Institute of Agrobiological Science, Tsukuba, Ibaraki 305-8602, Japan; National Agricultural Research Center for Hokkaido Region, Sapporo, Hokkaido 062-8555, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Ishimoto
authors.affiliation: Hokko Chemical Industry Co., Ltd., Atsugi, Kanagawa 243-0023, Japan.
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Terakawa
mesh_terms: D000544:Alzheimer Disease / Q000276:immunology / Q000517:prevention & control; D023582:Alzheimer Vaccines / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000595:Amino Acid Sequence; D004721:Endoplasmic Reticulum / Q000378:metabolism; D015870:Gene Expression; D005916:Globulins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010455:Peptides / Q000235:genetics / Q000276:immunology*; D030821:Plants, Genetically Modified; D021381:Protein Transport; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012639:Seeds / Q000235:genetics* / Q000378:metabolism; D030262:Soybean Proteins / Q000096:biosynthesis* / Q000235:genetics; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism; D014612:Vaccines; D014617:Vacuoles / Q000378:metabolism
chemical_list: D023582:Alzheimer Vaccines; D005916:Globulins; D010455:Peptides; D011994:Recombinant Proteins; D030262:Soybean Proteins; D014612:Vaccines; C011474:glycinin
keywords: Endoplasmic reticulum; Seed storage protein; Three dimensional structure; Vaccine; Vacuole