title: Novel approach to activity evaluation for release-active forms of anti-interferon-gamma antibodies based on enzyme-linked immunoassay.
abstract: Selection of a suitable assay to measure the activity of drug agents based on release-active forms of anti-interferon-gamma antibodies (RA forms of Abs) is an important step forward in the investigation of such agents. In this study, the enzyme-linked immunosorbent assay was utilized to examine the effect of RA forms of Abs specific for human interferon gamma on the interaction between monoclonal anti-interferon gamma antibodies and recombinant human interferon gamma. The experimental data and the results obtained by using relevant mathematical analysis showed that such RA forms of Abs are able to modulate the monoclonal antibody interaction with both soluble and immobilized (to the assay plate well) interferon gamma. These data demonstrated the importance of using relatively low concentrations of both soluble and plate-immobilized interferon gamma to detect the effects of RA forms of Abs to interferon gamma on the binding of monoclonal antibodies to interferon gamma. It has been suggested that the observed influence of RA forms of Abs on 'antibody-antigen' interaction could be used to detect and analyze the activity of drugs containing RA forms of Abs.
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", Moscow, Russian Federation.
authors.forename: Elena S
authors.initials: ES
authors.lastname: Gavrilova
authors.affiliation: Department of Molecular Immunology, Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kiev, Ukraine.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Bobrovnik
authors.affiliation: Ab Biotechnology Limited, Penicuik, United Kingdom.
authors.forename: Gordon
authors.initials: G
authors.lastname: Sherriff
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", Moscow, Russian Federation.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Myslivets
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", Moscow, Russian Federation.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Tarasov
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", Moscow, Russian Federation.
authors.forename: Oleg I
authors.initials: OI
authors.lastname: Epstein
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000276:immunology; D007371:Interferon-gamma / Q000737:chemistry / Q000276:immunology*; D012995:Solubility
chemical_list: D000911:Antibodies, Monoclonal; C573415:IFNG protein, human; D055767:Immobilized Proteins; D007371:Interferon-gamma