title: [Determination of dactylorhin A and militarine in three varieties of Cremastrae Pseudobulbus/Pleiones Pseudobulbus by HPLC].
abstract: To establish an HPLC method for determination of dactylorhin A and militarine in Cremastrae Pseudobulbus/Pleiones Pseudobulbus. The analysis was achieved on an Alltech Prevail C18 column (4. 6 mm x 250 mm, 5 microm) using a mobile phase of acetonitrile (A), water (B) gradient elution in a total run time of 35 min (0 min, 20:80; 30 min, 55:45; 35 min, 55:45) and a diode array detector was set at 224 nm. The flow rate was 0.8 mL x min(-1). The assay displayed good linearity over the concentration range of 0.257-9.95 microg (r = 0.999 8), and 0.128-10.27 microg (r = 0.999 9), respectively. The average recoveries (n = 9) were 94.70% and 102.8% for dactylorhin A and militarine, respectively. The method is accurate, quick, simple and reproducibility. It can be used for the quality control of Pleione bulbocodioides and Pleione yunnanensis.
authors.forename: Bao-Song
authors.initials: BS
authors.lastname: Cui
authors.forename: Jie
authors.initials: J
authors.lastname: Song
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.forename: Jian-Gong
authors.initials: JG
authors.lastname: Shi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000032:analysis*; D029595:Orchidaceae / Q000737:chemistry*; D013386:Succinates / Q000032:analysis*
chemical_list: D005960:Glucosides; D013386:Succinates; C000588443:dactylorhin A; C545907:militarine