title: UM-SCC-103: a unique tongue cancer cell line that recapitulates the tumorigenic stem cell population of the primary tumor.
abstract: OBJECTIVE
  A new head and neck cancer cell line was developed from a highly aggressive HNSCC of the oral cavity diagnosed in a 26-year-old pregnant woman.
  
  
  METHODS
  Cells from the primary tumor were passaged in culture and genotyped as a unique cell line. The resultant cell line was assessed for its ability to replicate the primary tumor.
  
  
  RESULTS
  The primary tumor and cell line contained 19.03% and 19.62% CD44(high) cells, respectively. CD44(high) cancer stem cells from UM-SCC-103 formed tumors after flank injections in mice that reconstituted the heterogeneity of the primary tumor. CD44 staining and histology in the primary tumor and tumors grown in vivo from the cell line were similar. CD44(high) cells from the primary tumor resulted in lung colony formation in 2 out of 2 tail vein injections in mice, whereas CD44(low) cells did not. Similarly, CD44(high) cells from UM-SCC-103 formed lung tumors in 2 out of 4 mice, whereas CD44(low) cells did not.
  
  
  CONCLUSIONS
  The similarity in marker expression and tumorigenic behavior between the primary tumor and the resulting cell line strongly suggests that the cell line resembles the primary tumor that it was derived from and provides an important new research tool for the study of head and neck carcinomas in young patients.
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: John H
authors.initials: JH
authors.lastname: Owen
authors.affiliation: Division of Otolaryngology-Head & Neck Surgery, University of California, San Diego, California, USA.
authors.forename: Samantha J
authors.initials: SJ
authors.lastname: Hauff
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, University of Cincinnati, Cincinnati, Ohio, USA.
authors.forename: Alice L
authors.initials: AL
authors.lastname: Tang
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: Martin P
authors.initials: MP
authors.lastname: Graham
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Czerwinski
authors.affiliation: Wayne State University Medical School, Detroit, Michigan, USA.
authors.forename: Marcella
authors.initials: M
authors.lastname: Kaddoura
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: Silvana
authors.initials: S
authors.lastname: Papagerakis
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: Carol R
authors.initials: CR
authors.lastname: Bradford
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Carey
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA mepp@umich.edu.
authors.forename: Mark E P
authors.initials: ME
authors.lastname: Prince
mesh_terms: D000328:Adult; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics*; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism; D011247:Pregnancy; D014062:Tongue Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors
keywords: CD44; aldehyde dehydrogenase; cancer stem cell; cell line; squamous cell carcinoma; tumorigenic