title: Assessing Pseudomonas aeruginosa Persister/antibiotic tolerant cells.
abstract: Bacterial persistence, which is observed in a broad range of microbial species, is the capacity of a bacterial cell subpopulation called "persisters" to tolerate exposure to normally lethal concentrations of bactericidal antibiotics. This ability, which is not due to antibiotic-resistant mutants, has been implicated in antibiotic treatment failures and may account for latent, chronic, and relapsing infections. Antibiotic tolerant/Persister (AT/P) cells have been notoriously difficult to study due to their low frequency and transient nature. This chapter describes the main methods used to isolate and study Pseudomonas aeruginosa AT/P cells and discusses new technologies that may ease research of P. aeruginosa persisters in the near future.
authors.affiliation: Department of Surgery, Harvard Medical School, Boston, MA, USA.
authors.forename: Ronen
authors.initials: R
authors.lastname: Hazan
authors.forename: Damien
authors.initials: D
authors.lastname: Maura
authors.forename: Yok Ai
authors.initials: YA
authors.lastname: Que
authors.forename: Laurence G
authors.initials: LG
authors.lastname: Rahme
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D050296:Microbial Viability; D009154:Mutation / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000187:drug effects* / Q000254:growth & development; D015203:Reproducibility of Results
chemical_list: D000900:Anti-Bacterial Agents