title: BL153 partially prevents high-fat diet induced liver damage probably via inhibition of lipid accumulation, inflammation, and oxidative stress.
abstract: The present study was to investigate whether a magnolia extract, named BL153, can prevent obesity-induced liver damage and identify the possible protective mechanism. To this end, obese mice were induced by feeding with high fat diet (HFD, 60% kcal as fat) and the age-matched control mice were fed with control diet (10% kcal as fat) for 6 months. Simultaneously these mice were treated with or without BL153 daily at 3 dose levels (2.5, 5, and 10 mg/kg) by gavage. HFD feeding significantly increased the body weight and the liver weight. Administration of BL153 significantly reduced the liver weight but without effects on body weight. As a critical step of the development of NAFLD, hepatic fibrosis was induced in the mice fed with HFD, shown by upregulating the expression of connective tissue growth factor and transforming growth factor beta 1, which were significantly attenuated by BL153 in a dose-dependent manner. Mechanism study revealed that BL153 significantly suppressed HFD induced hepatic lipid accumulation and oxidative stress and slightly prevented liver inflammation. These results suggest that HFD induced fibrosis in the liver can be prevented partially by BL153, probably due to reduction of hepatic lipid accumulation, inflammation and oxidative stress.
authors.affiliation: College of Bioengineering, Chongqing University, Chongqing 400044, China ; The Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Sciences & Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou 325035, China ; Department of Pediatrics of the University of Louisville, The Kosair Children's Hospital Research Institute, Louisville, KY 20202, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: The Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Sciences & Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou 325035, China ; Rui'an Center of the Chinese-American Research Institute for Diabetic Complications, The Third Affiliated Hospital of the Wenzhou Medical University, Wenzhou 325200, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics of the University of Louisville, The Kosair Children's Hospital Research Institute, Louisville, KY 20202, USA ; Department of Cardiology at the First Hospital & School of Public Health, Jilin University, Changchun 130021, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics of the University of Louisville, The Kosair Children's Hospital Research Institute, Louisville, KY 20202, USA ; Department of Cardiology at the First Hospital & School of Public Health, Jilin University, Changchun 130021, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: The Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Sciences & Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou 325035, China ; Rui'an Center of the Chinese-American Research Institute for Diabetic Complications, The Third Affiliated Hospital of the Wenzhou Medical University, Wenzhou 325200, China.
authors.forename: Xuemian
authors.initials: X
authors.lastname: Lu
authors.affiliation: The Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Sciences & Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou 325035, China ; Rui'an Center of the Chinese-American Research Institute for Diabetic Complications, The Third Affiliated Hospital of the Wenzhou Medical University, Wenzhou 325200, China.
authors.forename: Minglong
authors.initials: M
authors.lastname: Shao
authors.affiliation: The Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Sciences & Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou 325035, China ; Rui'an Center of the Chinese-American Research Institute for Diabetic Complications, The Third Affiliated Hospital of the Wenzhou Medical University, Wenzhou 325200, China.
authors.forename: Liangmiao
authors.initials: L
authors.lastname: Chen
authors.affiliation: The Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Sciences & Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou 325035, China ; Rui'an Center of the Chinese-American Research Institute for Diabetic Complications, The Third Affiliated Hospital of the Wenzhou Medical University, Wenzhou 325200, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: The Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Sciences & Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou 325035, China ; Rui'an Center of the Chinese-American Research Institute for Diabetic Complications, The Third Affiliated Hospital of the Wenzhou Medical University, Wenzhou 325200, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: The Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Sciences & Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou 325035, China ; Rui'an Center of the Chinese-American Research Institute for Diabetic Complications, The Third Affiliated Hospital of the Wenzhou Medical University, Wenzhou 325200, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
authors.affiliation: The Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Sciences & Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou 325035, China ; Department of Pediatrics of the University of Louisville, The Kosair Children's Hospital Research Institute, Louisville, KY 20202, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Bioland Biotec Co., Ltd., Zhangjiang Modern Medical Device Park, Pudong, Shanghai 201201, China.
authors.forename: Ki-Soo
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Bioland R&D Center, 59 Songjeongni 2-gil, Byeongcheon, Dongnam, Cheonan, Chungnam 330-863, Republic of Korea.
authors.forename: Ki Ho
authors.initials: KH
authors.lastname: Kim
authors.affiliation: College of Bioengineering, Chongqing University, Chongqing 400044, China.
authors.forename: Bochu
authors.initials: B
authors.lastname: Wang
authors.affiliation: Bioland R&D Center, 59 Songjeongni 2-gil, Byeongcheon, Dongnam, Cheonan, Chungnam 330-863, Republic of Korea.
authors.forename: Young Heui
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D059305:Diet, High-Fat; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D031566:Magnolia / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: C000593973:BL153 extract; D010936:Plant Extracts; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor