title: Preparation and biological evaluation of a novel selenium-containing exopolysaccharide from Rhizobium sp. N613.
abstract: In order to obtain a low toxic antitumor agent and an organic selenium source, an exopolysaccharide obtained from Rhizobium sp. N613 (REPS) was modified by selenious acid using barium chloride as the catalyst. The reaction conditions were optimized by response surface methodology (RSM), and the optimal conditions for preparation of selenium-containing REPS (Se-REPS) were obtained. The selenium content of Se-REPS was 790 μg/g under these conditions. The molecular structure of Se-REPS was confirmed by FTIR. In vitro antitumor activity of Se-REPS was evaluated by MTT assay, and the results indicated that Se-REPS could significantly inhibit the growth of S180 and HepG2 cells. Furthermore, Se-REPS exhibited comparable in vivo antitumor efficacy to cyclophosphamide at same concentrations. In addition, Se-REPS could substantially elevate the thymus and spleen indices in tumor-bearing mice. This study demonstrates that Se-REPS holds great potential to be a desirable antitumor agent for therapeutic and immunomodulatory applications.
authors.affiliation: Institute of Biotechnology, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, People's Repubic of China.
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Ding
authors.affiliation: Institute of Biotechnology, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, People's Repubic of China.
authors.forename: Rui-Hong
authors.initials: RH
authors.lastname: Nie
authors.affiliation: Institute of Biotechnology, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, People's Repubic of China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Lv
authors.affiliation: Shanxi Jincheng Anthracite Mining Group Co., Ltd., Jincheng 048006, People's Repubic of China.
authors.forename: Guo-Qin
authors.initials: GQ
authors.lastname: Wei
authors.affiliation: Institute of Biotechnology, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, People's Repubic of China. Electronic address: liangqi@sxu.edu.cn.
authors.forename: Liang-Qi
authors.initials: LQ
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D017609:Barium Compounds / Q000737:chemistry; D002384:Catalysis; D002712:Chlorides / Q000737:chemistry; D003520:Cyclophosphamide / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D011135:Polysaccharides, Bacterial / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D012231:Rhizobium / Q000737:chemistry*; D020887:Selenious Acid / Q000737:chemistry; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D017609:Barium Compounds; D002712:Chlorides; D011135:Polysaccharides, Bacterial; C024986:barium chloride; D003520:Cyclophosphamide; D020887:Selenious Acid
keywords: Antitumor activity; Immunomodulatory effect; Response surface methodology (RSM); Selenium-containing exopolysaccharide