title: Formation of self-assembled triple-layered rotavirus-like particles (tlRLPs) by constitutive co-expression of VP2, VP6, and VP7 in stably transfected high-five insect cell lines.
abstract: In this study, stable high-five insect cell line constitutively expressing rotavirus (RV) VP2 was co-transfected with VP6 and VP7-recombinant plasmids. The presence of RV proteins in stably transfected high-five cells was verified by molecular and protein analyses. To yield self-assembled triple-layered RV-like particles (tlRLPs), a stable insect high-five cell line was generated to produce RV VP6 and VP7 besides VP2. Self-assembled tlRLPs were observed by transmission electron microscopy (TEM), and enzyme-linked immunosorbent assay (ELISA) was used to assess their antigenicity in vivo. The results suggest that the stable transfected high-five cells are able to generate tlRLPs with the efficient antigenicity.
authors.affiliation: Virology Department, Pasteur Institute of Iran, Tehran, Iran; Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Zabihollah
authors.initials: Z
authors.lastname: Shoja
authors.forename: Maria
authors.initials: M
authors.lastname: Tagliamonte
authors.forename: Somayeh
authors.initials: S
authors.lastname: Jalilvand
authors.forename: Yaghoub
authors.initials: Y
authors.lastname: Mollaei-Kandelous
authors.forename: Angelo
authors.initials: A
authors.lastname: De Stradis
authors.forename: Maria Lina
authors.initials: ML
authors.lastname: Tornesello
authors.forename: Franco M
authors.initials: FM
authors.lastname: Buonaguro
authors.forename: Luigi
authors.initials: L
authors.lastname: Buonaguro
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D007313:Insecta; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D022243:Rotavirus Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D022701:Virosomes / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; D022243:Rotavirus Vaccines; C442545:VP2 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D058425:Vaccines, Virus-Like Particle; D022701:Virosomes
keywords: antigenicity; high-five insect cells; rotavirus VP2, VP6, and VP7; tlRLP