title: [Research progress of co-culture system for constructing vascularized tissue engineered bone].
abstract: OBJECTIVE
  To review the research progress of the co-culture system for constructing vascularized tissue engineered bone.
  
  
  METHODS
  The recent literature concerning the co-culture system for constructing vascularized tissue engineered bone was reviewed, including the selection of osteogenic and endothelial lineages, the design and surface modification of scaffolds, the models and dimensions of the co-culture system, the mechanism, the culture conditions, and their application progress.
  
  
  RESULTS
  The construction of vascularized tissue engineered bone is the prerequisite for their survival and further clinical application in vivo. Mesenchymal stem cells (owning the excellent osteogenic potential) and endothelial progenitor cells (capable of directional differentiation into endothelial cell) are considered as attractive cell types for the co-culture system to construct vascularized tissue engineered bone. The culture conditions need to be further optimized. Furthermore, how to achieve the clinical goals of minimal invasion and autologous transplantation also need to be further studied.
  
  
  CONCLUSIONS
  The strategy of the co-culture system for constructing vascularized tissue engineered bone would have a very broad prospects for clinical application in future.
authors.forename: Weili
authors.initials: W
authors.lastname: Fu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xiang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001842:Bone and Bones / Q000098:blood supply / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008954:Models, Biological; D010012:Osteogenesis; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials