title: [Cryoconservation of ovarian tissue: indications and outcome of the patients].
abstract: OBJECTIVE
  Ovarian Tissue Cryopreservation (OTC) is a very promising approach of fertility preservation for women and young patients who have to follow gonadotoxic treatments (chemotherapy, radiotherapy…). The aim of this study was to analyse the indications and the outcomes of the patients who had OTC in our center during the last 17 years.
  
  
  METHODS
  The study is retrospective. Forty-six patients, who underwent OTC in the Laboratory of Reproductive Biology of the University Hospital of Clermont-Ferrand, between January 1997 and December 2009, were included.
  
  
  RESULTS
  The average age on the day of ovarian tissue harvesting was 19.5 years. Fifty-two percent of the patients were minor. In order of decreasing frequency, the diseases for which OTC has been proposed were hematologic, ovarian tumors, sarcoma ou PNET and breast neoplasia. In 93.5 %, the harvesting of ovarian cortex was performed by laparoscopy. After OTC, 82.6 % of the patients were treated by chemotherapy. A bone marrow transplant was performed for 48 % of the study patients. At the time of data collection, 57 % of the patients who had evaluation of their ovarian function presented premature ovarian failure. Eight patients had one or more pregnancies after treatment. It was a natural pregnancy for five of them. The three others were obtained by medically assisted procreation (in vitro fertilization and oocyte donation).
  
  
  CONCLUSIONS
  We report a long-term follow-up of patients treated in our center for OTC. The originality of our study is to evaluate all aspects of OTC from the decision to propose the patients an OTC to their outcomes several years after the ovarian tissue harvesting. It is therefore a multidisciplinary approach both oncology, gynecological and pediatric whereas OTC is often considered restrictively in the literature. Finally, it seems to be essential to establish a specific medical care for these patients. This monitoring will allow an adequate assessment of pubertal development and ovarian function, management of estrogen deficiency and secondary infertility, supporting patients in their desire for motherhood.
authors.affiliation: Pôle de gynécologie-obstétrique-reproduction humaine, médecine de la reproduction, CHU de Clermont-Ferrand, CHU d'Estaing, 1, place Lucie-Aubrac, 63003 Clermont-Ferrand cedex 1, France. Electronic address: desvignes.fanny@neuf.fr.
authors.forename: F
authors.initials: F
authors.lastname: Desvignes
authors.affiliation: Pôle de gynécologie-obstétrique-reproduction humaine, médecine de la reproduction, CHU de Clermont-Ferrand, CHU d'Estaing, 1, place Lucie-Aubrac, 63003 Clermont-Ferrand cedex 1, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Pouly
authors.affiliation: Laboratoire AMP, CECOS, pôle de gynécologie-obstétrique-reproduction humaine, CHU de Clermont-Ferrand, CHU d'Estaing, 1, place Lucie-Aubrac, 63003 Clermont-Ferrand cedex 1, France.
authors.forename: L
authors.initials: L
authors.lastname: Janny
authors.affiliation: Pôle de gynécologie-obstétrique-reproduction humaine, médecine de la reproduction, CHU de Clermont-Ferrand, CHU d'Estaing, 1, place Lucie-Aubrac, 63003 Clermont-Ferrand cedex 1, France.
authors.forename: M
authors.initials: M
authors.lastname: Canis
authors.affiliation: Laboratoire AMP, CECOS, pôle de gynécologie-obstétrique-reproduction humaine, CHU de Clermont-Ferrand, CHU d'Estaing, 1, place Lucie-Aubrac, 63003 Clermont-Ferrand cedex 1, France.
authors.forename: S
authors.initials: S
authors.lastname: Sanfilippo
authors.affiliation: Centre régional de cancérologie et thérapie cellulaire pédiatrique, CHU d'Estaing, 1, place Lucie-Aubrac, 63003 Clermont-Ferrand cedex 1, France.
authors.forename: J
authors.initials: J
authors.lastname: Kanold
authors.affiliation: Département de chirurgie oncologique, centre Jean-Perrin, 58, rue Montalembert, 63000 Clermont-Ferrand, France.
authors.forename: G
authors.initials: G
authors.lastname: Lebouedec
authors.affiliation: Laboratoire AMP, CECOS, pôle de gynécologie-obstétrique-reproduction humaine, CHU de Clermont-Ferrand, CHU d'Estaing, 1, place Lucie-Aubrac, 63003 Clermont-Ferrand cedex 1, France.
authors.forename: F
authors.initials: F
authors.lastname: Brugnon
mesh_terms: D000293:Adolescent; D000328:Adult; D000970:Antineoplastic Agents / Q000009:adverse effects; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation; D005260:Female; D059247:Fertility Preservation / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000628:therapy*; D010053:Ovary; D011247:Pregnancy; D016649:Primary Ovarian Insufficiency / Q000139:chemically induced / Q000209:etiology; D011878:Radiotherapy / Q000009:adverse effects; D027724:Reproductive Techniques, Assisted; D020858:Tissue and Organ Harvesting; D055815:Young Adult
chemical_list: D000970:Antineoplastic Agents
keywords: Cryoconservation de tissu ovarien; Cryopreservation of ovarian tissue; Fertility preservation; Insuffisance ovarienne prématurée; Premature ovarian failure; Préservation de la fertilité