title: L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration.
abstract: Membrane rafts are microdomains of the plasma membrane that have multiple biological functions. The involvement of these structures in the biology of T cells, namely in signal transduction by the TCR, has been widely studied. However, the role of membrane rafts in immunoreceptor signaling in NK cells is less well known. We studied the distribution of the activating NKG2D receptor in lipid rafts by isolating DRMs in a sucrose density gradient or by raft fractionation by β-OG-selective solubility in the NKL cell line. We found that the NKG2D-DAP10 complex and pVav are recruited into rafts upon receptor stimulation. Qualitative proteomic analysis of these fractions showed that the actin cytoskeleton is involved in this process. In particular, we found that the actin-bundling protein L-plastin plays an important role in the clustering of NKG2D into lipid rafts. Moreover, coengagement of the inhibitory receptor NKG2A partially disrupted NKG2D recruitment into rafts. Furthermore, we demonstrated that L-plastin participates in NKG2D-mediated inhibition of NK cell chemotaxis.
authors.affiliation: Immunology and.
authors.forename: Esther
authors.initials: E
authors.lastname: Serrano-Pertierra
authors.affiliation: Neurology Departments, Hospital Universitario Central de Asturias, Oviedo, Asturias, Spain;
authors.forename: Eva
authors.initials: E
authors.lastname: Cernuda-Morollón
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic; and.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Brdička
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic; and.
authors.forename: Václav
authors.initials: V
authors.lastname: Hoøejši
authors.affiliation: Immunology and Fundación Renal "Íñigo Álvarez de Toledo", Madrid, Spain inmuno@hca.es.
authors.forename: Carlos
authors.initials: C
authors.lastname: López-Larrea
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D003902:Detergents / Q000494:pharmacology; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000378:metabolism*; D021962:Membrane Microdomains / Q000187:drug effects / Q000502:physiology*; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D046912:Multiprotein Complexes; D055654:NK Cell Lectin-Like Receptor Subfamily C / Q000378:metabolism; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000502:physiology*; D020543:Proteome; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000378:metabolism; D015398:Signal Transduction / Q000276:immunology
chemical_list: D003902:Detergents; C120528:HCST protein, human; C527085:KLRK1 protein, human; C047262:LCP1 protein, human; D008840:Microfilament Proteins; D046912:Multiprotein Complexes; D055654:NK Cell Lectin-Like Receptor Subfamily C; D055655:NK Cell Lectin-Like Receptor Subfamily K; D020543:Proteome; D034741:RNA, Small Interfering; D011971:Receptors, Immunologic
keywords: chemotaxis; membrane rafts