title: MicroRNA-24 modulates aflatoxin B1-related hepatocellular carcinoma prognosis and tumorigenesis.
abstract: MicroRNA-24 (miR-24) may be involved in neoplastic process; however, the role of this microRNA in the hepatocellular carcinoma (HCC) related to aflatoxin B1 (AFB1) has not been well elaborated. Here, we tested miR-24 expression in 207 pathology-diagnosed HCC cases from high AFB1 exposure areas and HCC cells. We found that miR-24 was upregulated in HCC tumor tissues relative to adjacent noncancerous tissue samples, and that the high expression of miR-24 was significantly correlated with larger tumor size, higher microvessel density, and tumor dedifferentiation. Additionally, this microRNA overexpression modified the recurrence-free survival (relative hazard ratio [HR], 4.75; 95% confidence interval [CI], 2.66-8.47) and overall survival (HR = 3.58, 95% CI = 2.34-5.46) of HCC patients. Furthermore, we observed some evidence of joint effects between miR-24 and AFB1 exposure on HCC prognosis. Functionally, miR-24 overexpression progressed tumor cells proliferation, inhibited cell apoptosis, and developed the formation of AFB1-DNA adducts. These results indicate for the first time that miR-24 may modify AFB1-related HCC prognosis and tumorigenesis.
authors.affiliation: Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Dongfang Road, No. 1630, Shanghai 200127, China.
authors.forename: Yi-Xiao
authors.initials: YX
authors.lastname: Liu
authors.affiliation: Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Dongfang Road, No. 1630, Shanghai 200127, China ; Department of Pathology, Youjiang Medical College for Nationalities, Baise 533000, China.
authors.forename: Xi-Dai
authors.identifier: 0000-0003-0960-0689
authors.initials: XD
authors.lastname: Long
authors.affiliation: Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Dongfang Road, No. 1630, Shanghai 200127, China.
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Xi
authors.affiliation: Department of Pathology, The Affiliated Tumor Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Yun
authors.identifier: 0000-0003-1994-862X
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Pathology, Youjiang Medical College for Nationalities, Baise 533000, China.
authors.forename: Xiao-Ying
authors.identifier: 0000-0003-3622-3452
authors.initials: XY
authors.lastname: Huang
authors.affiliation: Department of Pathology, Youjiang Medical College for Nationalities, Baise 533000, China.
authors.forename: Jin-Guang
authors.initials: JG
authors.lastname: Yao
authors.affiliation: Department of Medicine, The Affiliated Hospital of Youjiang Medical College for Nationalities, Baise 533000, China.
authors.forename: Chao
authors.identifier: 0000-0002-6720-6586
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Dongfang Road, No. 1630, Shanghai 200127, China.
authors.forename: Tian-Yu
authors.initials: TY
authors.lastname: Xing
authors.affiliation: Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Dongfang Road, No. 1630, Shanghai 200127, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000293:Adolescent; D000328:Adult; D016604:Aflatoxin B1 / Q000506:poisoning*; D000368:Aged; D063646:Carcinogenesis / Q000187:drug effects / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced* / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009183:Mycotoxins / Q000506:poisoning; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D009183:Mycotoxins; D016604:Aflatoxin B1