title: LC-MALDI-TOF/TOF for shotgun proteomics.
abstract: Automated liquid chromatography tandem mass spectrometry (LC-MS/MS) is a well-established technique for identification of components from complex mixtures in shotgun proteomics experiments. Approaches involving the use of matrix-assisted laser desorption/ionization mass spectrometry (LC-MALDI-MS/MS) comprise the preparation of protein extracts, their enzymatic digestion, the separation of the resulting peptides by nanoscale liquid chromatography coupled to a collector that deposits the microfractions onto a MALDI plate, and finally the mass spectrometry analysis of the fractions. Using an LC-MALDI strategy, the rate of the collection of MS/MS data is decoupled from the chromatographic separation, thus allowing high-quality data which are often complementary to electrospray ionization (LC-ESI-MS/MS) techniques.
authors.affiliation: Rheumatology Division, ProteoRed/ISCIII Proteomics Group INIBIC - Hospital Universitario de A Coruña, A Coruña, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Fernández-Puente
authors.forename: Jesús
authors.initials: J
authors.lastname: Mateos
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Blanco
authors.forename: Cristina
authors.initials: C
authors.lastname: Ruiz-Romero
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010455:Peptides; D011506:Proteins