title: Comparison of UHPLC-ESI-MS and Hadamard transform atmospheric pressure ion mobility-ESI-MS for rapid profiling of isomeric flavonoids.
abstract: Hadamard transform atmospheric pressure ion mobility-MS and rapid UHPLC-MS methods were investigated for analysis of closely related isomeric flavonoids and their glycosides using a test set of seven standards. On a time scale of a few minutes, the flavonoid aglycones were all separated by ion mobility, but not by UHPLC. The glycosides were better resolved by IMS but not completely separated by both methods. The results suggest that IMS provides sufficient resolution for separation of isomeric polyphenols such as flavonoids in high-throughput metabolomics studies.
authors.affiliation: Tofwerk AG, Uttigenstrasse 22, CH-3600 Thun, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Groessl
authors.affiliation: School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
authors.forename: Antonio
authors.initials: A
authors.lastname: Azzollini
authors.affiliation: School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
authors.forename: Philippe J
authors.initials: PJ
authors.lastname: Eugster
authors.affiliation: Tofwerk AG, Uttigenstrasse 22, CH-3600 Thun, Switzerland.
authors.forename: Benoit
authors.initials: B
authors.lastname: Plet
authors.affiliation: School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Wolfender
authors.affiliation: Tofwerk AG, Uttigenstrasse 22, CH-3600 Thun, Switzerland.
authors.forename: Richard
authors.initials: R
authors.lastname: Knochenmuss
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry*; D007536:Isomerism; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods*; D008968:Molecular Conformation; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D005419:Flavonoids