title: Relationship between urinary nickel and methylation of p15, p16 in workers exposed to nickel.
abstract: OBJECTIVE
  The purpose of this study was to investigate the relationship between urinary nickel and methylation of p15, p16 in workers exposed to nickel.
  
  
  METHODS
  In this study, 165 nickel-exposed workers and 67 workers without exposure were recruited. The levels of urinary nickel were analyzed using dimethylglyoxime spectrophotometric method. Methylation-specific polymerase chain reaction was used to detect the methylation of p15 and p16.
  
  
  RESULTS
  The median concentration of urinary nickel in the exposed group (4.58 μg/L) was significantly higher than that in the control group (1.78 μg/L; P < 0.01). The rate of methylation of p15 in the exposed group was significantly higher than that in the control group (P = 0.023). The multiple logistic analysis showed that workers having higher urinary nickel were at the higher risk of methylation of p15 (P = 0.024).
  
  
  CONCLUSIONS
  The levels of urinary nickel were significantly associated with the methylation of p15.
authors.affiliation: From the Department of Occupational Health (Dr Yang, Mr Chen, Mr Guo, and Mr Wang), School of Public Health, Shanxi Medical University, Taiyuan; and Centers for Disease Control and Prevention (Mr Li and Mr Sun), Taiyuan Iron and Steel (Group) Co Ltd, Shanxi, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yang
authors.forename: Wentao
authors.initials: W
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Jianya
authors.initials: J
authors.lastname: Sun
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Guo
authors.forename: Zhiwu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000235:genetics / Q000378:metabolism*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation / Q000187:drug effects*; D030541:Databases, Genetic; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009532:Nickel / Q000652:urine*; D016273:Occupational Exposure; D016133:Polymerase Chain Reaction
chemical_list: D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D009532:Nickel