title: Cyclosporine A improves adhesion and invasion of mouse preimplantation embryos via upregulating integrin β3 and matrix metalloproteinase-9.
abstract: Our previous study has demonstrated cyclosporin A (CsA) promotes the migration and invasiveness of human first-trimester trophoblast cells in vitro. Here, we further investigated the effect of CsA on the early implantation in vitro of mouse embryo. Female C57 mice were superovulated and mated, and then two-cell embryos were harvested from the oviducts and sequentially cultured in vitro in G1 and G2 media with 0, 0.1, 1.0 or 10 μM of CsA. Blastocyte formation, blastocyte cell number and apoptosis, embryo hatching were assessed in 4-6 dpc. The adhesion and stretching growth of hatched embryos in laminin coated dishes were evaluated from 5 dpc to 8 dpc, and the expressions of implantation serine proteinase 1 (ISP1), integrin (itg) β3 and matrix metalloproteinase (MMP)-9 were determined by real time PCR and immunofluorescence, respectively. We showed there was no significant difference in blastocyst formation rates, hatching rates, number of whole embryonic cells, apoptotic cells, and distribution of inner cell masses (ICMs) and trophoblasts (TB) between the CsA- and control-treated groups. Expression of ISP1 mRNA was unaffected on 5 dpc. After hatching, adhesion rate of 7 dpc significantly increased in 0.1 and 1.0 μM of CsA treatment, and embryo area of 8 dpc stretch growing on laminin were increased in 1.0 μM of CsA. The mRNA and protein expression of itgβ3 and MMP-9 on 7 dpc blastocyst were up-regulated. In conclusion, CsA in low dosage up-regulates itgβ3 and MMP-9 expression, and enhances embryonic adhesion and invasion, which is beneficial to the embryo implantation.
authors.affiliation: Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College Shanghai 200011, China ; Affiliated Hospital of Hainan Medical College Haikou, China.
authors.forename: Yuan-Hua
authors.initials: YH
authors.lastname: Huang
authors.affiliation: Affiliated Hospital of Hainan Medical College Haikou, China.
authors.forename: Yan-Lin
authors.initials: YL
authors.lastname: Ma
authors.affiliation: Affiliated Hospital of Hainan Medical College Haikou, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: Affiliated Hospital of Hainan Medical College Haikou, China ; Guiyang College of Traditional Chinese Medicine Guiyang, China.
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Mao
authors.affiliation: Affiliated Hospital of Hainan Medical College Haikou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College Shanghai 200011, China.
authors.forename: Mei-Rong
authors.initials: MR
authors.lastname: Du
authors.affiliation: Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College Shanghai 200011, China ; Affiliated Hospital of Hainan Medical College Haikou, China.
authors.forename: Da-Jin
authors.initials: DJ
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001755:Blastocyst / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D001757:Blastomeres / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D005260:Female; D007166:Immunosuppressive Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D039661:Integrin beta3 / Q000378:metabolism*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D012333:RNA, Messenger / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D007166:Immunosuppressive Agents; D039661:Integrin beta3; D012333:RNA, Messenger; D016572:Cyclosporine; C436412:Prss28 protein, mouse; D012697:Serine Endopeptidases; D020780:Matrix Metalloproteinase 9
keywords: Cyclosporine A; MMP; integrin; pre-implantation embryos