title: Effect of preservation conditions on cartilage tissue for cell transplantation.
abstract: Autologous chondrocyte transplantation involves isolating chondrocytes from a patient's cartilage tissue. Storage conditions such as storage time and temperature are important for the quality of the isolated cells. However, few studies have focused on variables for optimum tissue preservation, and there is neither an established method for storing cartilage nor reliable reports on how different conditions affect the isolated chondrocytes. Therefore, in the present study, we stored cartilage in various preservation solutions, under different temperatures, and for varying durations and determined their effects on the characteristics and viability of isolated chondrocytes. We assessed chondrocyte viability with the use of a cell proliferation assay and determined their chondrogenic potential with the use of reverse-transcription polymerase chain reaction, enzyme-linked immunosorbent assay, and glycosaminoglycan assays. Our results demonstrated that cartilage tissue stored in a preservation medium composed of dimethyl sulfoxide, fetal bovine serum, and Dulbecco Modified Eagle Medium at a ratio of 1:2:7 (v/v) or stored with a commercially available preservation solution generated greater numbers of chondrocytes when the storage temperature was -80°C than when it was 4°C. The viability of isolated cells decreased with time at 4°C, whereas these values remained constant for tissues stored at -80°C. Our data suggest that an optimal method for preserving cartilage tissue is required to ensure the quality of cells used for transplantation.
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology (IBST), Konkuk University, Seoul, Korea.
authors.forename: B Y
authors.initials: BY
authors.lastname: Kim
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology (IBST), Konkuk University, Seoul, Korea.
authors.forename: B M
authors.initials: BM
authors.lastname: Nam
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology (IBST), Konkuk University, Seoul, Korea.
authors.forename: K M
authors.initials: KM
authors.lastname: Lee
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology (IBST), Konkuk University, Seoul, Korea.
authors.forename: Y H
authors.initials: YH
authors.lastname: Jo
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology (IBST), Konkuk University, Seoul, Korea.
authors.forename: J G
authors.initials: JG
authors.lastname: Nemeno
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology (IBST), Konkuk University, Seoul, Korea.
authors.forename: W
authors.initials: W
authors.lastname: Yang
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology (IBST), Konkuk University, Seoul, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology (IBST), Konkuk University, Seoul, Korea.
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology (IBST), Konkuk University, Seoul, Korea.
authors.forename: I J
authors.initials: IJ
authors.lastname: Jang
authors.affiliation: Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Japan; Project Leader of Advanced Medical Research Center, Yokohama City University, Yokohama, Japan; PRESTO, Japan Science and Technology Agency, Kawaguchi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takebe
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology (IBST), Konkuk University, Seoul, Korea. Electronic address: jeongik@konkuk.ac.kr.
authors.forename: J I
authors.initials: JI
authors.lastname: Lee
mesh_terms: D000818:Animals; D064592:Autografts; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000637:transplantation; D020219:Chondrogenesis / Q000187:drug effects / Q000235:genetics; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000378:metabolism; D008297:Male; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D044967:Serum; D013997:Time Factors
chemical_list: D003470:Culture Media; D006025:Glycosaminoglycans; D019188:Organ Preservation Solutions; D012333:RNA, Messenger; D004121:Dimethyl Sulfoxide