title: Simultaneous imaging of cellular morphology and multiple biomarkers using an acousto-optic tunable filter-based bright field microscope.
abstract: An acousto-optic tunable filter (AOTF)-based multispectral imaging microscope system allows the combination of cellular morphology and multiple biomarker stainings on a single microscope slide. We describe advances in AOTF technology that have greatly improved spectral purity, field uniformity, and image quality. A multispectral imaging bright field microscope using these advances demonstrates pathology results that have great potential for clinical use.
authors.affiliation: Gooch and Housego, Lakewood, New Jersey 08701.
authors.forename: Elliot S
authors.initials: ES
authors.lastname: Wachman
authors.affiliation: Geyer Pathology Services, Pittsburgh, Pennsylvania 15238.
authors.forename: Stanley J
authors.initials: SJ
authors.lastname: Geyer
authors.affiliation: Gooch and Housego, Lakewood, New Jersey 08701.
authors.forename: Joel M
authors.initials: JM
authors.lastname: Recht
authors.affiliation: Gooch and Housego, Ilminster, Somerset TA19 0PF, United Kingdom.
authors.forename: Jon
authors.initials: J
authors.lastname: Ward
authors.affiliation: Gooch and Housego, Orlando, Florida 32811.
authors.forename: Bill
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Gooch and Housego, Palo Alto, California 93021.
authors.forename: Murray
authors.initials: M
authors.lastname: Reed
authors.affiliation: Gooch and Housego, Orlando, Florida 32811.
authors.forename: Chris
authors.initials: C
authors.lastname: Pannell
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D065006:Papanicolaou Test; D061088:Photoacoustic Techniques / Q000295:instrumentation / Q000379:methods*; D002583:Uterine Cervical Neoplasms
chemical_list: D014408:Biomarkers, Tumor