title: Anaerobic 4-chlorophenol mineralization in an enriched culture under iron-reducing conditions.
abstract: We enriched an anaerobic, soil-free 4-chlorophenol (4-CP)-degrading culture under iron-reducing conditions. The [ring-(14)C(U)]4-CP tracer experiment showed that 65 μM 4-CP mineralized to CO2 and CH4 through phenol, 4-hydroxybenzoate, and benzoate intermediates over 60 days. 16S rRNA gene analyses suggested the involvement of Dehalobacterium in the 4-CP dechlorination in the culture.
authors.affiliation: State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China; EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Li
authors.affiliation: EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Suzuki
authors.affiliation: EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Suyin
authors.initials: S
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Nan
authors.affiliation: Nagoya Institute of Technology, Nagoya 466-8555, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Yoshida
authors.affiliation: State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China.
authors.forename: Aijie
authors.initials: A
authors.lastname: Wang
authors.affiliation: EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan; Department of Civil Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan. Electronic address: aktym88@bd5.so-net.ne.jp.
authors.forename: Arata
authors.initials: A
authors.lastname: Katayama
mesh_terms: D000693:Anaerobiosis; D056449:Bacillales / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002733:Chlorophenols / Q000378:metabolism*; D054879:Halogenation; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D008697:Methane / Q000378:metabolism; D010084:Oxidation-Reduction; D010226:Parabens / Q000378:metabolism; D019800:Phenol / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D002733:Chlorophenols; D010226:Parabens; D012336:RNA, Ribosomal, 16S; D002245:Carbon Dioxide; D019800:Phenol; C029107:4-chlorophenol; D007501:Iron; C038193:4-hydroxybenzoic acid; D008697:Methane
keywords: (14)C-tracer experiment; 16S rRNA gene library analysis; 4-Chlorophenol; Anaerobic mineralization; Dehalobacterium