title: [Therapeutic effect of hepatic artery infusion with albendazole microspheres on hepatic alveolar echinococcosis in rats].
abstract: OBJECTIVE
  To observe therapeutic effect of hepatic artery infusion of albendazole solid dispersion-chitosan microspheres on hepatic alveolar echinococcosis (HAE) in rats.
  
  
  METHODS
  After the establishment of hepatic alveolar echinococcosis model, 30 rats were randomly divided into control group (A), blank microspheres group (B), and albendazole microspheres group (C) with 10 rats in each group 0.3 ml normal saline, 27mg/kg blank microspheres and 2.7 mg/kg albendazole solid dispersion-chitosan microspheres with 0.3 ml normal saline were injected through hepatic artery of rats in the groups of A, B and C, respectively. At 1 d, 3 d, 7 d, 14 d, and 42 d after injection, venous blood were collected, and white blood cells (WBC), aspartate aminotransferase (AST), and alanine aminotransferase(ALT) were evaluated. All the rats were sacrificed on 42 d after injection, and HAE pathological changes were observed.
  
  
  RESULTS
  Transient elevation of white blood cells was observed in all groups at 1 d after infusion [Group A (86.11 +/- 19.14) x 10(9)/L, B (117.11 +/- 21.76) x 10(9)/L, C (118.11 +/- 24.52) x 10(9)/L], at 7d WBC fell to normal level [A (7.85 +/- 6.57)x10(9)/L, B (11.73 +/- 4.85) x 10(9)/L, C (8.49 +/- 136)x10(9)/L]. In groups B and C, AST, ALT reached their peaks on day 3 after infusion [B: AST (193.15 +/- 21.57) U/L, ALT (78.39 +/- 9.78) U/L; C: AST (189.91 +/- 14.06) U/L, ALT (88.43 +/- 9.23) U/L], and decreased to normal level at 14d after infusion [B: AST (109.31 +/- 15.48) U/L, ALT(47.855 +/- 9.49) U/L; C: AST(105.37 +/- 8.16) U/L, ALT (49.53 +/- 6.75) UL]. Histopathological examination at 42d after infusion showed that in groups A and B structure of the cysts was virtually normal, but in group C most cysts showed necrosis in germinal layer.
  
  
  CONCLUSIONS
  Hepatic artery infusion with albendazole solid dispersion-chitosan microspheres shows certain therapeutic effect on hepatic alveolar echinococcosis in rats.
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Fan
authors.forename: Wei-Xin
authors.initials: WX
authors.lastname: Ren
authors.forename: Dilmurat
authors.initials: D
authors.lastname: Bawudun
authors.forename: Jun-Peng
authors.initials: JP
authors.lastname: Gu
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Xu
authors.forename: Hai-Xiao
authors.initials: HX
authors.lastname: Zhang
authors.forename: Wei-Zheng
authors.initials: WZ
authors.lastname: Ji
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Hao
authors.initials: H
authors.lastname: Wen
mesh_terms: D015766:Albendazole / Q000008:administration & dosage* / Q000627:therapeutic use; D000818:Animals; D004444:Echinococcosis, Hepatic / Q000188:drug therapy*; D005260:Female; D006499:Hepatic Artery; D007261:Infusions, Intra-Arterial; D008863:Microspheres; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015766:Albendazole