title: A genome-wide screen identifies Salmonella Enteritidis lipopolysaccharide biosynthesis and the HtrA heat shock protein as crucial factors involved in egg white persistence at chicken body temperature.
abstract: Eggs contaminated with Salmonella Enteritidis are an important source of human foodborne Salmonella infections. Salmonella Enteritidis is able to contaminate egg white during formation of the egg within the chicken oviduct, and it has developed strategies to withstand the antimicrobial properties of egg white to survive in this hostile environment. The mechanisms involved in the persistence of Salmonella Enteritidis in egg white are likely to be complex. To address this issue, a microarray-based transposon library screen was performed to identify genes necessary for survival of Salmonella Enteritidis in egg white at chicken body temperature. The majority of identified genes belonged to the lipopolysaccharide biosynthesis pathway. Additionally, we provide evidence that the serine protease/heat shock protein (HtrA) appears essential for the survival of Salmonella Enteritidis in egg white at chicken body temperature.
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: R
authors.initials: R
authors.lastname: Raspoet
authors.forename: N
authors.initials: N
authors.lastname: Shearer
authors.forename: C
authors.initials: C
authors.lastname: Appia-Ayme
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
authors.forename: R
authors.initials: R
authors.lastname: Ducatelle
authors.forename: A
authors.initials: A
authors.lastname: Thompson
authors.forename: F
authors.initials: F
authors.lastname: Van Immerseel
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001831:Body Temperature; D002645:Chickens; D004529:Egg White / Q000382:microbiology*; D055106:Genome-Wide Association Study / Q000662:veterinary; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000235:genetics*; D033903:Periplasmic Proteins / Q000235:genetics* / Q000378:metabolism; D011201:Poultry Diseases / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000235:genetics / Q000502:physiology*; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D008070:Lipopolysaccharides; D033903:Periplasmic Proteins; C061682:DegP protease; D012697:Serine Endopeptidases
keywords: HtrA; Salmonella Enteritidis; egg white; lipopolysaccharide; transposon library