title: Novel spectroscopic methods for determination of Cromolyn sodium and Oxymetazoline hydrochloride in binary mixture.
abstract: New accurate, sensitive and selective spectrophotometric and spectrofluorimetric methods were developed and subsequently validated for determination of Cromolyn sodium (CS) and Oxymetazoline HCl (OXY) in binary mixture. These methods include 'H-point standard addition method (HPSAM) and area under the curve (AUC)' spectrophotometric method and first derivative synchronous fluorescence spectroscopic (FDSFS) method. For spectrophotometric methods, absorbances were recorded at 241.5nm and 274.9nm for HPSAM and the wavelength was selected in ranges 232.0-254.0nm and 216.0-229.0nm for AUC method, where the concentration was obtained by applying Cramer's rule. For FDSFS method, the first-derivative synchronous fluorescence signal was measured at 290.0nm, using Δλ=145.0nm. The suggested methods were validated according to International Conference of Harmonization (ICH) guidelines and the results revealed that they were precise and reproducible. All the obtained results were statistically compared with those of the reported method and there was no significant difference.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt.
authors.forename: Omar
authors.initials: O
authors.lastname: Abdel-Aziz
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt.
authors.forename: A M
authors.initials: AM
authors.lastname: El-Kosasy
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt.
authors.forename: N
authors.initials: N
authors.lastname: Magdy
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt. Electronic address: noha_morsy_elzahar@hotmail.com.
authors.forename: N M
authors.initials: NM
authors.lastname: El Zahar
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000032:analysis*; D018927:Anti-Asthmatic Agents / Q000032:analysis*; D019540:Area Under Curve; D004205:Cromolyn Sodium / Q000032:analysis*; D057230:Limit of Detection; D010109:Oxymetazoline / Q000032:analysis*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000316:Adrenergic alpha-Agonists; D018927:Anti-Asthmatic Agents; D010109:Oxymetazoline; D004205:Cromolyn Sodium
keywords: Area under the curve; Cromolyn sodium; First derivative synchronous fluorescence spectroscopy; H-point standard addition method