title: Loop-mediated isothermal amplification (LAMP) method for detection of genetically modified maize T25.
abstract: The loop-mediated isothermal amplification (LAMP) assay indicates a potential and valuable means for genetically modified organism (GMO) detection especially for its rapidity, simplicity, and low cost. We developed and evaluated the specificity and sensitivity of the LAMP method for rapid detection of the genetically modified (GM) maize T25. A set of six specific primers was successfully designed to recognize six distinct sequences on the target gene, including a pair of inner primers, a pair of outer primers, and a pair of loop primers. The optimum reaction temperature and time were verified to be 65°C and 45 min, respectively. The detection limit of this LAMP assay was 5 g kg(-1) GMO component. Comparative experiments showed that the LAMP assay was a simple, rapid, accurate, and specific method for detecting the GM maize T25.
authors.affiliation: Food Inspection Center, Liaoning Entry-Exit Inspection and Quarantine Bureau Dalian, 116001, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Xu
authors.affiliation: Food Inspection Center, Liaoning Entry-Exit Inspection and Quarantine Bureau Dalian, 116001, China.
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Food Inspection Center, Liaoning Entry-Exit Inspection and Quarantine Bureau Dalian, 116001, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yu
authors.affiliation: Food Inspection Center, Liaoning Entry-Exit Inspection and Quarantine Bureau Dalian, 116001, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Liu
authors.affiliation: Food Inspection Center, Liaoning Entry-Exit Inspection and Quarantine Bureau Dalian, 116001, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Food Inspection Center, Liaoning Entry-Exit Inspection and Quarantine Bureau Dalian, 116001, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Life Science, Dalian polytechnic University Dalian, 116034, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Food Inspection Center, Liaoning Entry-Exit Inspection and Quarantine Bureau Dalian, 116001, China.
authors.forename: Jijuan
authors.initials: J
authors.lastname: Cao
keywords: GM maize T25; isothermal condition; loop-mediated isothermal amplification; rapid detection