title: Clinical Application of the DiversiLab Microbial Typing System Using Repetitive Sequence-Based PCR for Characterization of Helicobacter pylori in Japan.
abstract: We evaluated the DiversiLab (DL) system with universal primers, a semiautomated repetitive extragenic palindromic sequence-based polymerase chain reaction (PCR) (rep-PCR) system, for the characterization of Helicobacter pylori in Japan. All 135 isolates from Japanese patients with gastric cancer (GC, n = 55) or non-GC (n = 80) were used and subjected to the drug susceptibility examinations (amoxicillin, AMPC; metronidazole, MNZ; and clarithromycin, CAM) by E-test. There were 28 MNZ-resistant (20.7%), 35 CAM-resistant (25.9%), and 16 MNZ/CAM-resistant (11.9%) isolates. DL rep-PCR fingerprinting analysis at the level of 95% similarity revealed five major groups (A-E) and the other including 45 isolates. The occupation rates of GC-derived isolates in groups B (54.2%) and E (58.8%) were higher than in the other groups: A (26.7%), C (28.6%), D (30.0%), and the other (40.0%). Relative higher occupation rates of drug resistants, such as MNZ-, CAM- and double MNZ/CAM-resistant isolates, were observed in groups B (45.8%), C (42.6%), and D (40%). Five of eight GC-derived isolates with MNZ/CAM resistance were significantly assigned to group B (P = 0.0312, χ(2) -test). These results suggest that the isolates classified in group B have a potential to contribute to the development of severe gastric disorders. The DL system, rapid and high sensitive technology, would be widely available in clinical laboratory for pathological and epidemiological analyses even in H. pylori.
authors.affiliation: Department of Clinical Laboratory, Kochi Medical School Hospital, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, Japan.
authors.forename: Norihito
authors.initials: N
authors.lastname: Morimoto
authors.affiliation: Clinical Laboratory Medicine, Kochi University School of Medicine, Kochi, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeuchi
authors.affiliation: Department of Clinical Laboratory, Kochi Medical School Hospital, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, Japan.|Clinical Laboratory Medicine, Kochi University School of Medicine, Kochi, Japan.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Nishida
authors.affiliation: Department of Clinical Laboratory, Kochi Medical School Hospital, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, Japan.
authors.forename: Mie
authors.initials: M
authors.lastname: Morisawa
authors.affiliation: Department of Clinical Laboratory, Kochi Medical School Hospital, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, Japan.
authors.forename: Tomoe
authors.initials: T
authors.lastname: Yoshikawa
authors.affiliation: Department of Clinical Laboratory, Kochi Medical School Hospital, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, Japan.
authors.forename: Tamae
authors.initials: T
authors.lastname: Morita
authors.affiliation: Department of Clinical Laboratory, Kochi Medical School Hospital, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Morimoto
authors.affiliation: Clinical Laboratory Medicine, Kochi University School of Medicine, Kochi, Japan.
authors.forename: Chizuko
authors.initials: C
authors.lastname: Sugimoto
authors.affiliation: Clinical Laboratory Medicine, Kochi University School of Medicine, Kochi, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Matsumura
authors.affiliation: Department of Clinical Laboratory, Kochi Medical School Hospital, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, Japan.|Clinical Laboratory Medicine, Kochi University School of Medicine, Kochi, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Sugiura
mesh_terms: D016009:Chi-Square Distribution; D005260:Female; D016481:Helicobacter Infections / Q000150:complications / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D007564:Japan; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D013274:Stomach Neoplasms / Q000150:complications / Q000175:diagnosis / Q000382:microbiology
keywords: DiversiLab Microbial Typing System; Helicobacter pylori; antibiotic resistant; gastric cancer; genotyping; rep-PCR