title: Photopolymerization of polydiacetylene in hybrid liposomes: effect of polymerization on stability and response to pathogenic bacterial toxins.
abstract: Liposomes containing lipids and polydiacetylene (PDA) are hybrid systems encompassing both a fluid phospholipid membrane and a polymer scaffold (PDA). However, the biophysical role of PDA in such liposomes is not well understood. In this report, we studied the effects of photopolymerization of PDA on the stability of lipid-PDA liposomes, and their sensitivity to selected purified toxins and bacterial supernatants, using a fluorescence assay. Of the three different types of liposomes with variable lipid chain lengths that were chosen, the degree of polymerization had a significant impact on the long-term stability, and response, to external microbial exotoxins secreted by pathogenic bacteria, namely, Staphylococcus aureus and Pseudomonas aeruginosa. The degree of polymerization of TCDA played an important role in lipid-chain-length-dependent stabilization of lipid-PDA liposomes, as well as in their response to bacterial toxins of S. aureus and P. aeruginosa.
authors.affiliation: Department of Chemistry and ‡Department of Biology and Biochemistry, University of Bath , BA2 7AY, Bath, United Kingdom.
authors.forename: Naing Tun
authors.initials: NT
authors.lastname: Thet
authors.forename: William David
authors.initials: WD
authors.lastname: Jamieson
authors.forename: Maisem
authors.initials: M
authors.lastname: Laabei
authors.forename: June D
authors.initials: JD
authors.lastname: Mercer-Chalmers
authors.forename: A Toby A
authors.initials: AT
authors.lastname: Jenkins
mesh_terms: D001427:Bacterial Toxins / Q000737:chemistry* / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D000078789:Polyacetylene Polymer; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013211:Staphylococcus aureus / Q000378:metabolism; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: C434762:10,12-tricosadiynoic acid; D001427:Bacterial Toxins; D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D008055:Lipids; D008081:Liposomes; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D002784:Cholesterol