title: Differentiation of tonsil-tissue-derived mesenchymal stem cells controlled by surface-functionalized microspheres in PEG-polypeptide thermogels.
abstract: Poly(ethylene glycol)-poly(l-alanine) diblock copolymer (PEG-L-PA; molecular weight of each block of 1000-1080 Da) aqueous solutions undergo sol-to-gel transition in a 3.0-8.0 wt % concentration range as the temperature increases. By incorporating the polystyrene microspheres with different functional groups with a size of 100-800 μm in in situ formed PEG-L-PA thermogels, the differentiation of tonsil-tissue-derived mesenchymal stem cells (TMSCs) was investigated. The mRNA expression and immunohistochemical assays suggested that the TMSCs preferentially undergo adipogenesis in the ammonium (-NH3(+))- or thiol (-SH)-functionalized microsphere incorporated thermogels; chondrogenesis in the thiol-, phosphate (PO3(2-))-, or carboxylate (-COO(-))-functionalized microsphere incorporated thermogels; and osteogenesis in the phosphate-, carboxylate-functionalized, or neat polystyrene microsphere incorporated thermogels. This paper provides a new TMSC 3D culture system of a sol-gel reversible matrix and suggests that the surface-functional groups of microspheres in the thermogel can control the preferential differentiation of stem cells into specific cell types during the 3D culture.
authors.affiliation: Department of Chemistry and Nano Science, Ewha Global Top 5 Research Program, Ewha Womans University , 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Korea.
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Kye
authors.forename: Seung-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Park
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Moon
authors.forename: Kyung Ha
authors.initials: KH
authors.lastname: Ryu
authors.forename: Byeongmoon
authors.initials: B
authors.lastname: Jeong
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D005782:Gels; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology; D008863:Microspheres; D014066:Palatine Tonsil / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects
chemical_list: D005782:Gels; D010455:Peptides; C019529:polyalanine; D011092:Polyethylene Glycols