title: CDR3 analysis of TCR Vβ repertoire of CD8⁺ T cells from chickens infected with Eimeria maxima.
abstract: CD8(+) T cells play a major role in the immune protection of host against the reinfection of Eimeria maxima, the most immunogenic species of eimerian parasites in chickens. To explore the dominant complementarity-determining regions 3 (CDR3) of CD8(+) T cell populations induced by the infection of this parasite, sequence analysis was performed in this study for CDR3 of CD8(+) T cells from E. maxima infected chickens. After 5 days post the third or forth infection, intraepithelial lymphocytes were isolated from the jejunum of bird. CD3(+)CD8(+) T cells were sorted and subjected to total RNA isolation and cDNA preparation. PCR amplification and cloning of the loci between Vβ1 and Cβ was conducted for the subsequent sequencing of CDR3 of T cell receptor (TCR). After the forth infection, 2 birds exhibited two same frequent TCR CDR3 sequences, i.e., AKQDWGTGGYSNMI and AGRVLNIQY; while the third bird showed two different frequent TCR CDR3 sequences, AKQGARGHTPLN and AKQDIEVRGPNTPLN. No frequent CDR3 sequence was detected from uninfected birds, though AGRVLNIQY was also found in two uninfected birds. Our result preliminarily demonstrates that frequent CDR3 sequences may exist in E. maxima immunized chickens, encouraging the mining of the immunodominant CD8(+) T cells against E. maxima infection.
authors.affiliation: National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ren
authors.affiliation: National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Yin
authors.affiliation: National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Qin
authors.affiliation: National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Jingxia
authors.initials: J
authors.lastname: Suo
authors.affiliation: National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Qiyao
authors.initials: Q
authors.lastname: Lv
authors.affiliation: National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xie
authors.affiliation: National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Yunzhou
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Huang
authors.affiliation: National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: liuxianyong@cau.edu.cn.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Liu
authors.affiliation: National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Suo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002469:Cell Separation / Q000662:veterinary; D002645:Chickens / Q000276:immunology / Q000469:parasitology*; D003048:Coccidiosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D022801:Complementarity Determining Regions / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D004539:Eimeria / Q000276:immunology*; D005434:Flow Cytometry / Q000662:veterinary; D016056:Immunodominant Epitopes / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology*; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D013047:Specific Pathogen-Free Organisms
chemical_list: D022801:Complementarity Determining Regions; D018076:DNA, Complementary; D016056:Immunodominant Epitopes; D016053:RNA, Protozoan; D016693:Receptors, Antigen, T-Cell, alpha-beta
keywords: CDR3; Eimeria maxima; Immunodominant antigens; TCR repertoire