title: The role of the microenvironment in human immunodeficiency virus-associated classical Hodgkin lymphoma.
abstract: OBJECTIVE
  To characterize the microenvironment of classical Hodgkin lymphoma (cHL) in people living with human immunodeficiency virus (PLWH). The objective was to identify and then quantify the immune cells present in the microenvironment.
  
  
  RESULTS
  Ten samples of cHL from PLWH were compared with 10 samples of cHL from the general population using tissue microarray technology and immunohistochemistry. Sections were immunostained with antibodies for CD30, CD3, CD4, CD8, CD20, CD68R, CD56, CD57, CD123, FoxP3 and granzyme B. A statistically significant reduction of CD4(+) T cells, CD56(+) cells, CD57(+) cells, CD123(+) cells and B cells and an increase in numbers in FoxP3(+) CD8(+) cells was observed in cHL diagnosed in PLWH. No significant differences were seen in the number of CD8(+) T cells, CD4(+) FoxP3(+) T cells and macrophages.
  
  
  CONCLUSIONS
  There are considerable differences in the microenvironment of cHL occurring with and without HIV.
authors.affiliation: Imperial College, Department of Histopathology, Hammersmith Hospital Campus, London, UK.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Koulis
authors.forename: Pritesh
authors.initials: P
authors.lastname: Trivedi
authors.forename: Hazem
authors.initials: H
authors.lastname: Ibrahim
authors.forename: Mark
authors.initials: M
authors.lastname: Bower
authors.forename: Kikkeri N
authors.initials: KN
authors.lastname: Naresh
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006689:Hodgkin Disease / Q000276:immunology / Q000473:pathology* / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D046888:Tissue Array Analysis; D059016:Tumor Microenvironment / Q000276:immunology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD
keywords: classical Hodgkin lymphoma; human immunodeficiency virus; human immunodeficiency virus-associated classical Hodgkin lymphoma; immune system cells; inflammation; tumour microenvironment