title: Fluoroplast-polyaniline-coated adsorbent for one-step isolation of DNA for PCR detection of viral hepatitides (HBV and TTV).
abstract: OBJECTIVE
  To demonstrate the effectiveness of application of the adsorbent successively modified with nano-layers of fluoroplast and polyaniline for one-step isolation of DNA of hepatitis B virus and transfusion-transmitted virus from human serum.
  
  
  METHODS
  The technique is based on the application of the spin-cartridges containing porous adsorbent for one-step viral DNA isolation from serum followed by polymerase chain reaction.
  
  
  RESULTS
  The developed adsorbent was shown to be effective for one-step isolation of viral DNA from serum samples for polymerase chain reaction diagnostics.
  
  
  CONCLUSIONS
  The effectiveness of the developed adsorbent application for isolation of viral DNA from serum for polymerase chain reaction diagnostics was confirmed in comparison with standard methods. Thus, the facile sample preparation method of viral DNA isolation was elaborated.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya St., 16/10, 117997, Moscow, Russia. kapustin@ibch.ru.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Kapustin
authors.forename: Anna I
authors.initials: AI
authors.lastname: Prostyakova
authors.forename: Vitaly P
authors.initials: VP
authors.lastname: Zubov
mesh_terms: D000327:Adsorption; D017848:Blood-Borne Pathogens; D004279:DNA, Viral / Q000032:analysis*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004279:DNA, Viral