title: [Endoplasmic reticulum stress mediates lipopolysaccharide-induced apoptosis in rat hepatocyte].
abstract: OBJECTIVE
  To investigate the role of endoplasmic reticulum stress (ERS) in lipopolysaccharide (LPS)-induced hepatocyte apoptosis.
  
  
  METHODS
  Cells of the rat hepatocyte line BRL were cultured. The hepatocytes were treated with LPS, ERS inducer thapsigargin (TG), and ERS inhibitor 4-phenylbutyric acid (4-PBA), respectively or in their different combination. The cell viability was measured by MTT assay. The cyto-nuclear morphological changes of apoptosis cells were detected by the fluorescent dye Hoechst 33258. The apoptosis rate was assessed by flow cytometry with Annexin V-FITC/PI double-staining. Expressions of GRP78 as ERS marker protein, CHOP, caspase-12 and cleaved-caspase-3 as ERS related protein were detected by Western blotting.
  
  
  RESULTS
  LPS could cause a decrease in cell viability and an increase in apoptosis rate in a dose- and time-dependent manner. The expression of GRP78, CHOP, caspase-12 and cleaved-caspase-3 proteins were significantly increased with LPS treatment. TG led to a marked decrease in cell viability and an increase in apoptosis rate, which aggravated the hepatocyte injury induced by LPS; whereas 4-PBA alleviated LPS-induced apoptosis.
  
  
  CONCLUSIONS
  ERS mediates LPS-induced hepatocyte injuries, indicating that ERS may play a vital role in the pathogenesis of LPS-induced hepatocyte injuries.
authors.forename: Ying-Lei
authors.initials: YL
authors.lastname: Ji
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Yan-Sha
authors.initials: YS
authors.lastname: Wang
authors.forename: Yi-Chang
authors.initials: YC
authors.lastname: Liu
authors.forename: Zhen-Yong
authors.initials: ZY
authors.lastname: Gu
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D002470:Cell Survival; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress; D006360:Heat-Shock Proteins; D022781:Hepatocytes; D008070:Lipopolysaccharides; D010654:Phenylbutyrates; D051381:Rats
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D008070:Lipopolysaccharides; D010654:Phenylbutyrates; C075773:4-phenylbutyric acid; D053148:Caspase 3