title: [Analysis of Toxoplasma gondii specific antigens by two dimensional electrophoresis and western blotting].
abstract: The total protein of Toxoplasma gondii RH strain tachyzoites was separated by two-dimensional electrophoresis (2-DE), and Western blotting was performed to find out distinct antigens. 209 spots were detected through Coomassie brilliant blue-stained small gels (7x8 cm, pH 3-10). Western blotting showed 17 specific antigen spots with sera of the experimental group and two nonspecific spots with the control sera.
authors.forename: Lin-Jie
authors.initials: LJ
authors.lastname: Li
authors.forename: Huai-En
authors.initials: HE
authors.lastname: Bao
authors.forename: Wei
authors.initials: W
authors.lastname: Pan
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017207:Rats, Sprague-Dawley; D014122:Toxoplasma / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan