title: Comparative assessment of DNA-based approaches for the quantification of food allergens.
abstract: Governments all over the world have implemented regulatory frameworks concerning food allergen labelling and established or discussed the implementation of thresholds. Therefore, quantitative methods are needed for their surveillance. DNA-based approaches using a matrix-adapted calibration, an internal standard material and a modified standard addition have been developed. In order to enable a comparative assessment of the available quantification methods, experimental framework conditions and uniform performance criteria were defined. For the evaluation of the experimental results using homogenous sample material, the recovery, repeatability and reproducibility were considered along with the limit of detection and the limit of quantification. In addition, muffin dough and muffins spiked with sesame were analysed to assess the suitability of the methods to quantify sesame in model foods. The modified standard addition emerged from the comparative assessment and the analysis of the model foods to be the most appropriate method to quantify traces of allergens in food.
authors.affiliation: Technische Universität München, Lehrstuhl für Allgemeine Lebensmitteltechnologie, Maximus-von-Imhof-Forum 2, D-85350 Freising-Weihenstephan, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Luber
authors.affiliation: Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (LGL), Veterinärstraße 2, D-85764 Oberschleißheim, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Demmel
authors.affiliation: Technische Universität München, Lehrstuhl für Allgemeine Lebensmitteltechnologie, Maximus-von-Imhof-Forum 2, D-85350 Freising-Weihenstephan, Germany.
authors.forename: Denise
authors.initials: D
authors.lastname: Herbert
authors.affiliation: Technische Universität München, Lehrstuhl für Allgemeine Lebensmitteltechnologie, Maximus-von-Imhof-Forum 2, D-85350 Freising-Weihenstephan, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Hosken
authors.affiliation: Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (LGL), Veterinärstraße 2, D-85764 Oberschleißheim, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Hupfer
authors.affiliation: Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (LGL), Veterinärstraße 2, D-85764 Oberschleißheim, Germany.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Huber
authors.affiliation: Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (LGL), Veterinärstraße 2, D-85764 Oberschleißheim, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Busch
authors.affiliation: Technische Universität München, Lehrstuhl für Allgemeine Lebensmitteltechnologie, Maximus-von-Imhof-Forum 2, D-85350 Freising-Weihenstephan, Germany. Electronic address: k.h.engel@wzw.tum.de.
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Engel
mesh_terms: D000485:Allergens / Q000032:analysis*; D018744:DNA, Plant / Q000032:analysis; D005504:Food Analysis / Q000379:methods; D005512:Food Hypersensitivity / Q000209:etiology*; D057230:Limit of Detection; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D031685:Sesamum / Q000737:chemistry*
chemical_list: D000485:Allergens; D018744:DNA, Plant
keywords: Food allergens; Internal standard material; Matrix-adapted quantification; Modified standard addition; Quantification; Real-time PCR