title: Derivation of ES-like cell from neonatal mouse testis cells in autologous sertoli cells co-culture system.
abstract: BACKGROUND
  Spermatogonial stem cell (SSC) is a self-renewing population of male adult stem cell. SSCs have a differentiation potential which are similar to embryonic stem cells. These Embryonic stem like (ES-like) cells can be a potential source for pluripotent cells for stem cell-based therapy.
  
  
  OBJECTIVE
  This study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testis Materials and Methods: Isolated testicular cells were cultured in DMEM/F12. Characteristics of the isolated cells and obtained ES-like cell were immune-cytochemically confirmed by examining the presence of PLZF, vimentin, Oct4 and Nanog protein. Expression of the pluripotency and germ-cell specific genes was analyzed by qPCR in derived ES-like colony and SSCs respectively.
  
  
  RESULTS
  The experiment results indicated that our method of obtaining pluripotent ES-like cells from spermatogonial cells (SCs) is simpler than the described methods. ES-like cells were immunopositive for pluripotency markers. ES-like cell qPCR results indicated signiﬁcant increase in pluripotency genes expression and signiﬁcant decrease in germ cell-speciﬁc genes expression.
  
  
  CONCLUSIONS
  The results indicated that ES-like cell with pluripotency characteristic were generated from freshly isolated spermatogonial cells. The pluripotent stem cells provide a cellular reservoir usable for regenerative medicine instead of embryonic stem cells. This article extracted from Ph.D. thesis. (Setareh Javanmardi).
authors.affiliation: Department of Anatomical Sciences, Faculty of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Asadi
authors.affiliation: Department of Anatomical Sciences, Faculty of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Setareh
authors.initials: S
authors.lastname: Javanmardi
authors.affiliation: Department of Anatomical Sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Movahedin
keywords: Co-culture; Embryonic stem like cell; Pluripotent stem cells; Spermatogonial stem cells