title: Towards error-free profiling of immune repertoires.
abstract: Deep profiling of antibody and T cell-receptor repertoires by means of high-throughput sequencing has become an attractive approach for adaptive immunity studies, but its power is substantially compromised by the accumulation of PCR and sequencing errors. Here we report MIGEC (molecular identifier groups-based error correction), a strategy for high-throughput sequencing data analysis. MIGEC allows for nearly absolute error correction while fully preserving the natural diversity of complex immune repertoires.
authors.affiliation: 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Pirogov Russian National Research Medical University, Moscow, Russia. [3].
authors.forename: Mikhail
authors.identifier: 0000000178267942
authors.initials: M
authors.lastname: Shugay
authors.affiliation: 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2].
authors.forename: Olga V
authors.initials: OV
authors.lastname: Britanova
authors.affiliation: 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2].
authors.forename: Ekaterina M
authors.initials: EM
authors.lastname: Merzlyak
authors.affiliation: 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2].
authors.forename: Maria A
authors.initials: MA
authors.lastname: Turchaninova
authors.affiliation: 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
authors.forename: Ilgar Z
authors.initials: IZ
authors.lastname: Mamedov
authors.affiliation: Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia.
authors.forename: Timur R
authors.initials: TR
authors.lastname: Tuganbaev
authors.affiliation: Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia.
authors.forename: Dmitriy A
authors.initials: DA
authors.lastname: Bolotin
authors.affiliation: Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia.
authors.forename: Dmitry B
authors.initials: DB
authors.lastname: Staroverov
authors.affiliation: Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Putintseva
authors.affiliation: Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
authors.forename: Karla
authors.initials: K
authors.lastname: Plevova
authors.affiliation: Division of Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
authors.forename: Carsten
authors.initials: C
authors.lastname: Linnemann
authors.affiliation: 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Pirogov Russian National Research Medical University, Moscow, Russia.
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Shagin
authors.affiliation: Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
authors.forename: Sarka
authors.initials: S
authors.lastname: Pospisilova
authors.affiliation: 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Pirogov Russian National Research Medical University, Moscow, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Lukyanov
authors.affiliation: Division of Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
authors.forename: Ton N
authors.initials: TN
authors.lastname: Schumacher
authors.affiliation: 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Pirogov Russian National Research Medical University, Moscow, Russia. [3] Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
authors.forename: Dmitriy M
authors.identifier: 000000030430790X
authors.initials: DM
authors.lastname: Chudakov
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods* / Q000592:standards; D059014:High-Throughput Nucleotide Sequencing / Q000592:standards*; D016133:Polymerase Chain Reaction / Q000592:standards; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012107:Research Design
chemical_list: D011948:Receptors, Antigen, T-Cell