title: LC-MS/MS quantitative analysis of quorum sensing signal molecules.
abstract: Extracts taken from spent growth media from Pseudomonas aeruginosa can be analyzed for N-acyl-L-homoserine lactones and 2-alkyl-4-(1H)-quinolones (AQs), including the known quorum sensing signalling molecules of P. aeruginosa, in a specific and sensitive manner by liquid chromatography coupled with tandem mass spectrometric detection. This analysis can be conducted in a quantitative manner by comparison with matrix-matched calibration samples.
authors.affiliation: Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
authors.forename: Catharine A
authors.initials: CA
authors.lastname: Ortori
authors.forename: Nigel
authors.initials: N
authors.lastname: Halliday
authors.forename: Miguel
authors.initials: M
authors.lastname: Cámara
authors.forename: Paul
authors.initials: P
authors.lastname: Williams
authors.forename: David A
authors.initials: DA
authors.lastname: Barrett
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011786:Quality Control; D015363:Quinolones / Q000737:chemistry; D053038:Quorum Sensing; D012015:Reference Standards; D015398:Signal Transduction; D012997:Solvents; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015363:Quinolones; D012997:Solvents