title: Molecular cloning and expression analysis of pig CD7.
abstract: CD7 is an integral membrane protein which mediates an important signal to mediate the differentiation, activation, and regulation of some T cells and NK cells. However, only human and mouse CD7 have been identified and studied among mammalian species. In this study, we cloned pig CD7 cDNA and determined its complete cDNA sequence. Pig CD7 cDNA contained an open reading frame (627 bp) encoding 208 amino acids with well conserved motifs involved in signal transduction within cytoplasmic tail among mammalian species. Pig CD7 mRNA was detected by RT-PCR in mainly lymphoid tissues, indicating the conserved functions of CD7 in pigs. Moreover, we generated soluble pig CD7 fusion immunoglobulin (pig CD7Ig) containing extracellular domain of pig CD7 to test whether pig CD7 binds to pig galectin-3. Flow cytometry and immunohistochemistry analyses indicated that soluble pig CD7Ig can bind to galectin-3 expressed in macrophages and epithelial cells of small intestine. These results help to analyze the structural relationship between CD7 and its ligand transferring signal transduction among mammalian species.
authors.affiliation: Division of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Republic of Korea.
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Lee
authors.forename: Joonbeom
authors.initials: J
authors.lastname: Bae
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
authors.forename: Chung-Gyu
authors.initials: CG
authors.lastname: Park
authors.forename: Taehoon
authors.initials: T
authors.lastname: Chun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019011:Antigens, CD7 / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D037502:Galectin 3 / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008221:Lymphoid Tissue / Q000276:immunology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013552:Swine / Q000145:classification / Q000235:genetics* / Q000276:immunology*
chemical_list: D019011:Antigens, CD7; D018076:DNA, Complementary; D037502:Galectin 3