title: Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL.
abstract: We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.
authors.affiliation: 1] RIKEN SPring-8 Center, Sayo, Japan. [2] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [3].
authors.forename: Kunio
authors.initials: K
authors.lastname: Hirata
authors.affiliation: 1] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [2].
authors.forename: Kyoko
authors.initials: K
authors.lastname: Shinzawa-Itoh
authors.affiliation: 1] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [2] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
authors.forename: Naomine
authors.initials: N
authors.lastname: Yano
authors.affiliation: Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Takemura
authors.affiliation: 1] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [2].
authors.forename: Koji
authors.initials: K
authors.lastname: Kato
authors.affiliation: Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Hatanaka
authors.affiliation: Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Muramoto
authors.affiliation: Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Kawahara
authors.affiliation: 1] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [2] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [3] Institute for Protein Research, Osaka University, Suita, Japan.
authors.forename: Tomitake
authors.initials: T
authors.lastname: Tsukihara
authors.affiliation: Institute for Protein Research, Osaka University, Suita, Japan.
authors.forename: Eiki
authors.initials: E
authors.lastname: Yamashita
authors.affiliation: Japan Synchrotron Radiation Research Institute, Sayo, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Tono
authors.affiliation: RIKEN SPring-8 Center, Sayo, Japan.
authors.forename: Go
authors.initials: G
authors.lastname: Ueno
authors.affiliation: RIKEN SPring-8 Center, Sayo, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Hikima
authors.affiliation: RIKEN SPring-8 Center, Sayo, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Murakami
authors.affiliation: RIKEN SPring-8 Center, Sayo, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Inubushi
authors.affiliation: RIKEN SPring-8 Center, Sayo, Japan.
authors.forename: Makina
authors.initials: M
authors.lastname: Yabashi
authors.affiliation: RIKEN SPring-8 Center, Sayo, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ishikawa
authors.affiliation: RIKEN SPring-8 Center, Sayo, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: Picobiology Institute, Graduate School of Life Science, University of Hyogo, Sayo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ogura
authors.affiliation: RIKEN SPring-8 Center, Sayo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugimoto
authors.affiliation: Photosynthesis Research Center, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
authors.forename: Jian-Ren
authors.initials: JR
authors.lastname: Shen
authors.affiliation: Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Yoshikawa
authors.affiliation: RIKEN SPring-8 Center, Sayo, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Ago
mesh_terms: D000818:Animals; D002417:Cattle; D003461:Crystallography / Q000379:methods*; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000528:radiation effects; D007834:Lasers
chemical_list: D003576:Electron Transport Complex IV