title: Immunosuppressive activity of daphnetin, one of coumarin derivatives, is mediated through suppression of NF-κB and NFAT signaling pathways in mouse T cells.
abstract: Daphnetin, a plant-derived dihydroxylated derivative of coumarin, is an effective compound extracted from a plant called Daphne Korean Nakai. Coumarin derivates were known for their antithrombotic, anti-inflammatory, and antioxidant activities. The present study was aimed to determine the immunosuppressive effects and the underlying mechanisms of daphnetin on concanavalin A (ConA) induced T lymphocytes in mice. We showed that, in vitro, daphnetin suppressed ConA-induced splenocyte proliferation, influenced production of the cytokines and inhibited cell cycle progression through the G0/G1 transition. The data also revealed that daphnetin could down-regulate activation of ConA induced NF-κB and NFAT signal transduction pathways in mouse T lymphocyte. In vivo, daphnetin treatment significantly inhibited the 2, 4- dinitrofluorobenzene (DNFB) -induced delayed type hypersensitivity (DTH) reactions in mice. Collectively, daphnetin had strong immunosuppressive activity both in vitro and in vivo, suggesting a potential role for daphnetin as an immunosuppressive agent, and established the groundwork for further research on daphnetin.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, People's Republic of China.
authors.forename: Bocui
authors.initials: B
authors.lastname: Song
authors.affiliation: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, People's Republic of China.
authors.forename: Zhenning
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, People's Republic of China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, People's Republic of China.
authors.forename: Guoren
authors.initials: G
authors.lastname: Huang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, People's Republic of China.
authors.forename: Linli
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, People's Republic of China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, People's Republic of China; Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Guan
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A / Q000276:immunology; D003374:Coumarins / Q000276:immunology* / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects / Q000276:immunology; D016193:G1 Phase / Q000187:drug effects / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007166:Immunosuppressive Agents / Q000276:immunology* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000276:immunology*; D050778:NFATC Transcription Factors / Q000276:immunology*; D016192:Resting Phase, Cell Cycle / Q000187:drug effects / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014468:Umbelliferones / Q000276:immunology* / Q000494:pharmacology
chemical_list: D003374:Coumarins; D007166:Immunosuppressive Agents; D016328:NF-kappa B; D050778:NFATC Transcription Factors; D014468:Umbelliferones; D003208:Concanavalin A; C030123:coumarin; C039952:daphnetin