title: Integrated analysis of gene network in childhood leukemia from microarray and pathway databases.
abstract: Glucocorticoids (GCs) have been used as therapeutic agents for children with acute lymphoblastic leukaemia (ALL) for over 50 years. However, much remains to be understood about the molecular mechanism of GCs actions in ALL subtypes. In this study, we delineate differential responses of ALL subtypes, B- and T-ALL, to GCs treatment at systems level by identifying the differences among biological processes, molecular pathways, and interaction networks that emerge from the action of GCs through the use of a selected number of available bioinformatics methods and tools. We provide biological insight into GC-regulated genes, their related functions, and their networks specific to the ALL subtypes. We show that differentially expressed GC-regulated genes participate in distinct underlying biological processes affected by GCs in B-ALL and T-ALL with little to no overlap. These findings provide the opportunity towards identifying new therapeutic targets.
authors.affiliation: Centre for Advanced Computational Solutions (CfACS), Lincoln University, Lincoln 7647, New Zealand ; Division of Science and Math, New York University Abu Dhabi and Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi Institute, P.O. Box 129188, Abu Dhabi, UAE.
authors.forename: Amphun
authors.initials: A
authors.lastname: Chaiboonchoe
authors.affiliation: Centre for Advanced Computational Solutions (CfACS), Lincoln University, Lincoln 7647, New Zealand ; Integrated Systems Modelling Group, Lincoln University, Lincoln 7647, New Zealand.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Samarasinghe
authors.affiliation: Centre for Advanced Computational Solutions (CfACS), Lincoln University, Lincoln 7647, New Zealand ; Department of Wine, Food & Molecular Biosciences, Lincoln University, Lincoln 7647, New Zealand.
authors.forename: Don
authors.initials: D
authors.lastname: Kulasiri
authors.affiliation: Division of Science and Math, New York University Abu Dhabi and Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi Institute, P.O. Box 129188, Abu Dhabi, UAE.
authors.forename: Kourosh
authors.initials: K
authors.lastname: Salehi-Ashtiani
mesh_terms: D030541:Databases, Genetic; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics; D053263:Gene Regulatory Networks / Q000187:drug effects / Q000235:genetics; D005938:Glucocorticoids / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma
chemical_list: D005938:Glucocorticoids