title: Correlation between MMP-9 and extracellular cytokine HMGB1 in prediction of human ischemic stroke outcome.
abstract: Ischemic stroke (IS) outcome predictors include clinical features, biochemical parameters and some risk factors. The relations between two main players in the ischemic brain, MMPs and HMGB1, were estimated in the plasma of ischemic stroke patients stratified according to the Glasgow Outcome Scale and the Oxfordshire Community Stroke Project classification. IS patients exhibited higher plasma concentration of MMP-9 and the inflammatory cytokine HMGB1 compared with healthy controls. A full-blown correlation between MMP-9 activation and increased plasma MMP-9 concentration was observed in case of IS patients. A similar activity of MMP-2 and MMP-12 was characteristic of healthy volunteers and IS patients. In patients with ischemic stroke increased plasma levels of MMP-9 and HMGB1 are associated with a poor functional outcome and are significantly correlated with each other (P=0.0054). We suggest that diagnostic benefits will be obtained if plasma HMGB1 levels are measured for IS patients in addition to MMP-9.
authors.affiliation: I. Javakhishvili Tbilisi State University, Andronikashvili Institute of Physics, 6 Tamarashvili Str., 0177 Tbilisi, Georgia. Electronic address: n.sapojnikova@aiphysics.ge.
authors.forename: Nelly
authors.initials: N
authors.lastname: Sapojnikova
authors.affiliation: I. Javakhishvili Tbilisi State University, Andronikashvili Institute of Physics, 6 Tamarashvili Str., 0177 Tbilisi, Georgia.
authors.forename: Tamar
authors.initials: T
authors.lastname: Kartvelishvili
authors.affiliation: I. Javakhishvili Tbilisi State University, Andronikashvili Institute of Physics, 6 Tamarashvili Str., 0177 Tbilisi, Georgia.
authors.forename: Nino
authors.initials: N
authors.lastname: Asatiani
authors.affiliation: School of Pharmacology and Biomedical Sciences, University of Portsmouth, St Michael's Building, White Swan Road, Portsmouth PO1 2DT, UK.
authors.forename: Vitaly
authors.initials: V
authors.lastname: Zinkevich
authors.affiliation: Faculty of Medicine, Department of Neurology and Neurosurgery, I. Javakhishvili Tbilisi State University, 1, Chavchavadze Ave, 0179 Tbilisi, Georgia.
authors.forename: Iagor
authors.initials: I
authors.lastname: Kalandadze
authors.affiliation: Faculty of Medicine, Department of Neurology and Neurosurgery, I. Javakhishvili Tbilisi State University, 1, Chavchavadze Ave, 0179 Tbilisi, Georgia.
authors.forename: Darejan
authors.initials: D
authors.lastname: Gugutsidze
authors.affiliation: Faculty of Medicine, Department of Neurology and Neurosurgery, I. Javakhishvili Tbilisi State University, 1, Chavchavadze Ave, 0179 Tbilisi, Georgia.
authors.forename: Roman
authors.initials: R
authors.lastname: Shakarishvili
authors.affiliation: Faculty of Medicine, Department of Neurology and Neurosurgery, I. Javakhishvili Tbilisi State University, 1, Chavchavadze Ave, 0179 Tbilisi, Georgia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Tsiskaridze
mesh_terms: D000208:Acute Disease; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D015153:Blotting, Western; D002545:Brain Ischemia / Q000097:blood / Q000175:diagnosis*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D024243:HMGB1 Protein / Q000097:blood*; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D011379:Prognosis; D020521:Stroke / Q000097:blood / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D024243:HMGB1 Protein; C585491:HMGB1 protein, human; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Gelatin zymography; HMGB1; Human ischemic stroke; Matrix metalloproteinases