title: Prophylactic and therapeutic effects of Acanthopanax senticosus Harms extract on murine collagen-induced arthritis.
abstract: Evidences are accumulating that extract of Acanthopanax senticosus Harms (ASH; syn Eleutherococcus senticosus [Rupr. & Maxim.] Maxim), a shrub native to Northeastern Asia, has antiinflammatory effects. In this study, we examined prophylactic and therapeutic effects of ASH extract (ASHE) on rheumatoid arthritis using collagen-induced arthritis (CIA) mouse model. Acanthopanax senticosus Harms extract was administered before the onset of arthritis in the prophylaxis model. In the therapeutic model, ASHE was administered after the onset of arthritis with or without anti-TNF-α antibody. The ASHE treatment showed efficacy before onset of CIA but there was no effect after CIA was established. The ASHE treatment delayed the onset and decreased severity of CIA. In vitro examinations showed that ASHE is an antioxidant and that ASHE suppresses TNF-α and interleukin-6 production in human peripheral blood mononuclear cells. The combination therapy with ASHE and anti-TNF-α antibody reduced the severity of arthritis compared with anti-TNF-α antibody alone. The present study shows that ASHE has prophylactic effect against CIA and support therapeutic effect of anti-TNF-α antibody.
authors.affiliation: Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Maki
authors.initials: M
authors.lastname: Tanaka
authors.forename: Ryosei
authors.initials: R
authors.lastname: Murai
authors.forename: Kageaki
authors.initials: K
authors.lastname: Kuribayashi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kobayashi
authors.forename: Nozomi
authors.initials: N
authors.lastname: Yanagihara
authors.forename: Naoki
authors.initials: N
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D018501:Antirheumatic Agents / Q000494:pharmacology; D001169:Arthritis, Experimental / Q000188:drug therapy*; D001172:Arthritis, Rheumatoid; D004195:Disease Models, Animal; D028001:Eleutherococcus / Q000737:chemistry*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010936:Plant Extracts / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D018501:Antirheumatic Agents; C508600:IL6 protein, human; D015850:Interleukin-6; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha
keywords: Acanthopanax senticosus Harms; anti-TNF-α antibody; collagen-induced arthritis; inflammatory cytokines; reactive oxygen species