title: Analysis of lipid peroxidation biomarkers in extremely low gestational age neonate urines by UPLC-MS/MS.
abstract: Extremely low gestational age neonates (ELGAN) frequently require the use of oxygen supply in the delivery room leading to systemic inflammation and oxidative stress that are responsible for increased morbidity and mortality. The objective of this study was to establish reference ranges of a set of representative isoprostanes and prostaglandins, which are stable biomarkers of lipid peroxidation often correlated with oxidative stress-related disorders. First, a quantitative ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated. The proposed analytical method was tailored for its application in the field of neonatology, enabling multi-analyte detection in non-invasive, small-volume urine samples. Then, the lipid peroxidation product concentrations in a total of 536 urine samples collected within the framework of two clinical trials including extremely low gestational age neonates (ELGAN) were analyzed. The access to a substantially large number of samples from this very vulnerable population provided the chance to establish reference ranges of the studied biomarkers. Up to the present, and for this population, this is the biggest reference data set reported in literature. Results obtained should assist researchers and pediatricians in interpreting test results in future studies involving isoprostanes and prostaglandins, and could help assessing morbidities and evaluate effectiveness of treatment strategies (e.g., different resuscitation conditions) in the neonatal field.
authors.affiliation: Neonatal Research Group, Health Research Institute Hospital La Fe, Bulevar Sur s/n, 46026, Valencia, Spain.
authors.forename: Julia
authors.initials: J
authors.lastname: Kuligowski
authors.forename: Javier
authors.initials: J
authors.lastname: Escobar
authors.forename: Guillermo
authors.initials: G
authors.lastname: Quintás
authors.forename: Isabel
authors.initials: I
authors.lastname: Lliso
authors.forename: Isabel
authors.initials: I
authors.lastname: Torres-Cuevas
authors.forename: Antonio
authors.initials: A
authors.lastname: Nuñez
authors.forename: Elena
authors.initials: E
authors.lastname: Cubells
authors.forename: Denise
authors.initials: D
authors.lastname: Rook
authors.forename: Johannes B
authors.initials: JB
authors.lastname: van Goudoever
authors.forename: Máximo
authors.initials: M
authors.lastname: Vento
mesh_terms: D015415:Biomarkers / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D062071:Infant, Extremely Premature / Q000652:urine*; D007231:Infant, Newborn; D028421:Isoprostanes / Q000652:urine*; D015227:Lipid Peroxidation; D008297:Male; D011453:Prostaglandins / Q000652:urine*; D016032:Randomized Controlled Trials as Topic; D012016:Reference Values; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers; D028421:Isoprostanes; D011453:Prostaglandins