title: The complete mitochondrial genome sequence of Hepatozoon catesbianae (Apicomplexa: Coccidia: Adeleorina), a blood parasite of the green frog, Lithobates (formerly Rana) clamitans.
abstract: A complete mitochondrial genome for the blood parasite Hepatozoon catesbianae (Alveolata; Apicomplexa; Coccidia; Adeleorina; Hepatozoidae) was obtained through PCR amplification and direct sequencing of resulting PCR products. The mitochondrial genome of H. catesbianae is 6,397 bp in length and contains 3 protein-coding genes (cytochrome c oxidase subunit I [COI]; cytochrome c oxidase subunit III [COIII]; and cytochrome B [CytB]). Sequence similarities to previously published mitochondrial genomes of other apicomplexan parasites permitted annotation of 23 putative rDNA fragments in the mitochondrial genome of H. catesbianae, 14 large subunit rDNA fragments, and 9 small subunit rDNA fragments. Sequences corresponding to rDNA fragments RNA5, RNA8, RNA11, and RNA19 of Plasmodium falciparum were not identified in the mitrochondrial genome sequence of H. catesbianae. Although the presence of 3 protein-coding regions and numerous putative rDNA fragments is a feature typical for apicomplexan mitochondrial genomes, the mitochondrial genome of H. catesbianae possesses a structure and gene organization that is distinct among the Apicomplexa. This is the first complete mitochondrial genome sequence obtained from any apicomplexan parasite in the suborder Adeleorina.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1 Canada.
authors.forename: Alexandre N
authors.initials: AN
authors.lastname: Leveille
authors.forename: Mosun E
authors.initials: ME
authors.lastname: Ogedengbe
authors.forename: Mian A
authors.initials: MA
authors.lastname: Hafeez
authors.forename: Hsiang-Hsien Abby
authors.initials: HH
authors.lastname: Tu
authors.forename: John R
authors.initials: JR
authors.lastname: Barta
mesh_terms: D000818:Animals; D003044:Coccidia / Q000235:genetics*; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D045303:Cytochromes b / Q000235:genetics; D017931:DNA Primers; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry; D003576:Electron Transport Complex IV / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D004912:Erythrocytes / Q000469:parasitology; D054629:Genome, Mitochondrial / Q000235:genetics*; D018503:Genome, Protozoan / Q000235:genetics*; D058977:Molecular Sequence Annotation; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D063188:Rana clamitans / Q000097:blood / Q000469:parasitology*; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D045303:Cytochromes b; D003576:Electron Transport Complex IV