title: Molecular screening of virulence genes in extraintestinal pathogenic Escherichia coli isolated from human blood culture in Brazil.
abstract: Extraintestinal pathogenic Escherichia coli (ExPEC) is one of the main etiological agents of bloodstream infections caused by Gram-negative bacilli. In the present study, 20 E. coli isolates from human hemocultures were characterized to identify genetic features associated with virulence (pathogenicity islands markers, phylogenetic group, virulence genes, plasmid profiles, and conjugative plasmids) and these results were compared with commensal isolates. The most prevalent pathogenicity island, in strains from hemoculture, were PAI IV536, described by many researchers as a stable island in enterobacteria. Among virulence genes, iutA gene was found more frequently and this gene enconding the aerobactin siderophore receptor. According to the phylogenetic classification, group B2 was the most commonly found. Additionally, through plasmid analysis, 14 isolates showed plasmids and 3 of these were shown to be conjugative. Although in stool samples of healthy people the presence of commensal strains is common, human intestinal tract may serve as a reservoir for ExPEC.
authors.affiliation: Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, 86051-980 Londrina, PR, Brazil.
authors.forename: Vanessa L
authors.initials: VL
authors.lastname: Koga
authors.affiliation: Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, 86051-980 Londrina, PR, Brazil.
authors.forename: Geizecler
authors.identifier: 0000-0001-9530-2746
authors.initials: G
authors.lastname: Tomazetto
authors.affiliation: Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, 86051-980 Londrina, PR, Brazil.
authors.forename: Paula S
authors.initials: PS
authors.lastname: Cyoia
authors.affiliation: Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, 86051-980 Londrina, PR, Brazil.
authors.forename: Meiriele S
authors.initials: MS
authors.lastname: Neves
authors.affiliation: Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, 86051-980 Londrina, PR, Brazil.
authors.forename: Marilda C
authors.initials: MC
authors.lastname: Vidotto
authors.affiliation: Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, 86051-980 Londrina, PR, Brazil.
authors.forename: Gerson
authors.initials: G
authors.lastname: Nakazato
authors.affiliation: Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, 86051-980 Londrina, PR, Brazil.
authors.forename: Renata K T
authors.initials: RK
authors.lastname: Kobayashi
mesh_terms: D000293:Adolescent; D000328:Adult; D016470:Bacteremia / Q000382:microbiology*; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors