title: Production of recombinant protein in Escherichia coli cultured in extract from waste product alga, Ulva lactuca.
abstract: This study examined the potential for waste product alga, Ulva lactuca, to serve as a media component for recombinant protein production in Escherichia coli. To facilitate this investigation, U. lactuca harvested from Jamaica Bay was dried, and nutrients acid extracted for use as a growth media. The E. coli cell line BL21(DE3) was used to assess the effects on growth and production of recombinant green fluorescent protein (GFP). This study showed that media composed of acid extracts without further nutrient addition maintained E. coli growth and recombinant protein production. Extracts made from dried algae lots less than six-months-old were able to produce two-fold more GFP protein than traditional Lysogeny Broth media.
authors.affiliation: Ralph E. Martin Dept. of Chemical Engineering, University of Arkansas, Fayetteville, AR, 72701.
authors.forename: Tammy M
authors.initials: TM
authors.lastname: Rechtin
authors.forename: Matthew
authors.initials: M
authors.lastname: Hurst
authors.forename: Tom
authors.initials: T
authors.lastname: Potts
authors.forename: Jamie
authors.initials: J
authors.lastname: Hestekin
authors.forename: Robert
authors.initials: R
authors.lastname: Beitle
authors.forename: John
authors.initials: J
authors.lastname: McLaughlin
authors.forename: Peter
authors.initials: P
authors.lastname: May
mesh_terms: D001688:Biological Products / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D044445:Ulva / Q000737:chemistry / Q000378:metabolism*; D014866:Waste Products
chemical_list: D001688:Biological Products; D003470:Culture Media; D011994:Recombinant Proteins; D014866:Waste Products; D049452:Green Fluorescent Proteins
keywords: Escherichia coli; Ulva lactuca; green fluorescent protein; recombinant protein