title: Rapid determination of natural and synthetic hormones in biosolids and poultry manure by isotope dilution GC-MS/MS.
abstract: The release of hormones into the environment due to land application of biosolids and manure is a cause of concern for their potential impacts. This paper presents the development of a rapid and sensitive method, based on extraction, for the analysis of 13 hormones in biosolids and poultry manure. A simultaneous derivatization of hydroxyl and ketone groups was carried out for the determination of hormones by GC–MS/MS. The method was validated in three matrices (sewage sludge, manure, and broiler litter). Recoveries from spiked samples at three concentration levels (50, 25, and 10 ng/g) ranged from 76 to 124% with relative SDs ≤ 16%. Method detection limits for the three matrices were in the range of 0.5–3.0 ng/g dry weight. The optimized method was applied to biosolid and poultry manure samples collected in Spain. Only seven of the 13 studied hormones were detected in the different samples. trans-Androsterone was detected at high levels (up to 3.1 μg/g in biosolid samples). Estrone and estradiol were the two hormones detected at higher levels in layer manure, whereas estrone and 4-androstene-3,17-dione presented the highest levels in broiler litter.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Albero
authors.forename: Consuelo
authors.initials: C
authors.lastname: Sánchez-Brunete
authors.forename: Esther
authors.initials: E
authors.lastname: Miguel
authors.forename: Ramón
authors.initials: R
authors.lastname: Aznar
authors.forename: José L
authors.initials: JL
authors.lastname: Tadeo
mesh_terms: D001688:Biological Products / Q000032:analysis*; D002849:Chromatography, Gas; D006728:Hormones / Q000032:analysis*; D007201:Indicator Dilution Techniques; D007553:Isotope Labeling; D008372:Manure / Q000032:analysis*; D012989:Soil Pollutants / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D001688:Biological Products; D006728:Hormones; D008372:Manure; D012989:Soil Pollutants