title: [Effect of Aβ1-42 injection on hippocampus cells in rats and protective role of polygona-polysaccharose for Alzheimer's disease].
abstract: OBJECTIVE
  To determine the effect of polygona-polysaccharose (PP) on learning and memory ability in rats with Alzheimer's disease (AD).
  
  
  METHODS
  Forty five Sprague-Dawley rats were assigned into 3 groups. Rats in the sham-operated group were injected with normal saline. Rats in the Aβ group were injected with Aβ1-42. Rats in the PP group were injected with 16% PP solution for 45 days consecutively. The Morris water maze was used to investigate the ability of learning and memory in the rats. The effect of Aβ and PP on the hippocampus cells was observed by HE and Congo red staining of methanol.
  
  
  RESULTS
  Rats in the sham-operated group had no obvious morphological change; and morphology of rats in the PP group was basicaly normal. The layer of pyramidal cells in the Aβ group was decreased. The cells appeared sparse and irregular and became smaller. Karyopyknosis and vacuolar degeneration cells were also found. More positive staining materials aggradated in the Aβ group compared with the PP group by Congo red staining (P<0.05).
  
  
  CONCLUSIONS
  Aβ infusion into the hippocampus results in the impairment of the neuronal degeneration in the rats, which shows similar characterizations of AD. PP can reduce the deposition of Aβ in the hippocampus.
authors.affiliation: Department of Gerontology, Second Xiangya Hospital, Central South University, Changsha 410011, China 13508488911@163.com.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Yi
authors.forename: Shixing
authors.initials: S
authors.lastname: Wu
authors.forename: Maosheng
authors.initials: M
authors.lastname: Ye
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Xie
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy*; D016229:Amyloid beta-Peptides / Q000009:adverse effects*; D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal; D006624:Hippocampus / Q000166:cytology / Q000473:pathology*; D008568:Memory / Q000187:drug effects*; D010446:Peptide Fragments / Q000009:adverse effects*; D028502:Polygonum / Q000737:chemistry; D011134:Polysaccharides / Q000494:pharmacology*; D017966:Pyramidal Cells / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016229:Amyloid beta-Peptides; D004365:Drugs, Chinese Herbal; D010446:Peptide Fragments; D011134:Polysaccharides; C075222:amyloid beta-protein (1-42)