title: Graphene-based nanobiocatalytic systems: recent advances and future prospects.
abstract: Graphene-based nanomaterials are particularly useful nanostructured materials that show great promise in biotechnology and biomedicine. Owing to their unique structural features, exceptional chemical, electrical, and mechanical properties, and their ability to affect the microenvironment of biomolecules, graphene-based nanomaterials are suitable for use in various applications, such as immobilization of enzymes. We present the current advances in research on graphene-based nanomaterials used as novel scaffolds to build robust nanobiocatalytic systems. Their catalytic behavior is affected by the nature of enzyme-nanomaterial interactions and, thus, the availability of methods to couple enzymes with nanomaterials is an important issue. We discuss the implications of such interactions along with future prospects and possible challenges in this rapidly developing area.
authors.affiliation: Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, 45110, Ioannina, Greece; Institute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Felix-Hausdorff-Str. 4, D-17487, Greifswald, Germany.
authors.forename: Ioannis V
authors.initials: IV
authors.lastname: Pavlidis
authors.affiliation: Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, 45110, Ioannina, Greece.
authors.forename: Michaela
authors.initials: M
authors.lastname: Patila
authors.affiliation: Institute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Felix-Hausdorff-Str. 4, D-17487, Greifswald, Germany.
authors.forename: Uwe T
authors.initials: UT
authors.lastname: Bornscheuer
authors.affiliation: Department of Material Sciences and Engineering, University of Ioannina, 45110, Ioannina, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Gournis
authors.affiliation: Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, 45110, Ioannina, Greece. Electronic address: hstamati@cc.uoi.gr.
authors.forename: Haralambos
authors.initials: H
authors.lastname: Stamatis
mesh_terms: D019149:Bioreactors; D004800:Enzymes, Immobilized / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*
chemical_list: D004800:Enzymes, Immobilized; D006108:Graphite
keywords: biofuel cells; enzyme immobilization; enzyme–nanomaterial interactions; graphene-based nanomaterials; nanobiocatalysis