title: Crystallization and preliminary X-ray crystallographic analysis of the XoGroEL chaperonin from Xanthomonas oryzae pv. oryzae.
abstract: Along with the co-chaperonin GroES, the chaperonin GroEL plays an essential role in enhancing protein folding or refolding and in protecting proteins against misfolding and aggregation in the cellular environment. The XoGroEL gene (XOO_4288) from Xanthomonas oryzae pv. oryzae was cloned and the protein was expressed, purified and crystallized. The purified XoGroEL protein was crystallized using the hanging-drop vapour-diffusion method and a crystal diffracted to a resolution of 3.4 Å. The crystal belonged to the orthorhombic space group P212121 with 14 monomers in the asymmetric unit, with a corresponding VM of 2.7 Å(3) Da(-1) and a solvent content of 54.5%.
authors.affiliation: Department of Advanced Technology Fusion, Konkuk University, Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Huyen Thi
authors.initials: HT
authors.lastname: Tran
authors.affiliation: Department of Advanced Technology Fusion, Konkuk University, Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Tan Viet
authors.initials: TV
authors.lastname: Pham
authors.affiliation: Department of Biological Sciences, Konkuk University, Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Ho Phuong Thuy
authors.initials: HP
authors.lastname: Ngo
authors.affiliation: Department of Biological Sciences, Konkuk University, Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Myoung Ki
authors.initials: MK
authors.lastname: Hong
authors.affiliation: Genomics Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
authors.forename: Jeong Gu
authors.initials: JG
authors.lastname: Kim
authors.affiliation: National Leading Research Laboratory, Department of Biological Sciences, Myongji University, 116 Myongjiro, Yongin, Gyeonggido 449-728, Republic of Korea.
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Life Science, College of Natural Sciences, Sangmyung University, Seoul 110-743, Republic of Korea.
authors.forename: Yeh Jin
authors.initials: YJ
authors.lastname: Ahn
authors.affiliation: Department of Biological Sciences, Konkuk University, Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Lin Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D018833:Chaperonins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D014974:Xanthomonas
chemical_list: D001426:Bacterial Proteins; D018833:Chaperonins
keywords: GroEL; Xanthomonas oryzae pv. oryzae (Xoo); chaperone