title: Association between miR-146aG>C and miR-196a2C>T polymorphisms and the risk of hepatocellular carcinoma in a Chinese population.
abstract: MicroRNAs have been demonstrated to have a role in susceptibility and prognosis of various types of human cancer. We investigated the association between polymorphisms in miR-146aG>C, miR-196a2C>T, and miR-499A>G and hepatocellular carcinoma (HCC) risk and interaction with HCC and hepatitis B virus (HBV) infection. Two hundred sixty-six cases with HCC and 281 health controls were enrolled in the present study. Genotyping of the miR-146aG>C, miR-196a2C>T, and miR-499A>G genotypes was conducted by duplex polymerase chain reaction with the confronting two-pair primer (PCR-RFLP). Subjects with miR-146a GG and G allele had an increased risk of HCC compared with the homozygote CC genotype. Similarly, HCC patients carrying microRNA (miRNA)-196a2 computed tomography, TT, and T allele significantly decreased the risk of HCC relative to the CC genotype. Stratified analysis indicated that miR-196a2C>T polymorphism was associated with reduced risk of HBV-related HCC, but not in hepatitis C virus- and nonviral-related HCC cases. In conclusion, miR-146aG>C and miR-196a2C>T polymorphism are associated with risk of HCC patients in China, especially in patients with HBV infection. SNPs in miRNA sequences can be used as a diagnostic biomarker for HCC.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui, 453100, China, bingzhou_xmu@163.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhou
authors.forename: Liang-Peng
authors.initials: LP
authors.lastname: Dong
authors.forename: Xiao-Yue
authors.initials: XY
authors.lastname: Jing
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Li
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Yang
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Wang
authors.forename: Long-Feng
authors.initials: LF
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000209:etiology / Q000235:genetics*; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008113:Liver Neoplasms / Q000209:etiology / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012306:Risk
chemical_list: C542542:MIRN146 microRNA, human; C542543:MIRN196 microRNA, human; D035683:MicroRNAs