title: Effect of storage temperature and antibiotic impregnation on the quantity of bone morphogenetic protein seven in human bone grafts.
abstract: OBJECTIVE
  The aim of this study was to quantify the amount of bone morphogenic protein 7 (BMP-7) in bone samples in different storage and treatment conditions used in bone banks and thereby evaluate the benefit of this test as a routine measure before bone grafting.
  
  
  METHODS
  Fresh as well as frozen bone chips, each with and without antibiotic impregnation, were screened for their BMP-7 content. Human bone chips were produced from femoral heads of two female donors who had undergone total hip replacement surgery. The amount of BMP-7 was detected using a commercially available enzyme-linked immunosorbent assay (ELISA) test.
  
  
  RESULTS
  There were no significant differences between groups in samples obtained from the first femoral head. Bone-chip samples derived from the second femoral head showed significant differences between groups. The actual amount of these differences was small and most likely biologically irrelevant. It is important to note that there was a significant difference between groups when comparing both femoral heads, reflecting donor-to-donor variability.
  
  
  CONCLUSIONS
  ELISA testing for BMP-7 as a qualitative measurement of bone grafts should be considered a routine quality-control test for bone banks.
authors.affiliation: Experimental Orthopaedics, Medical University of Innsbruck, Salurnerstrasse 15, 6020, Innsbruck, Austria.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wurm
authors.forename: Michael
authors.initials: M
authors.lastname: Nogler
authors.forename: Christoph G
authors.initials: CG
authors.lastname: Ammann
authors.forename: Débora C
authors.initials: DC
authors.lastname: Coraça-Huber
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D055419:Bone Morphogenetic Protein 7 / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005270:Femur Head / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D013696:Temperature; D014021:Tissue Preservation
chemical_list: D000900:Anti-Bacterial Agents; D055419:Bone Morphogenetic Protein 7