title: Role of evolutionarily conserved signaling intermediate in Toll pathways (ECSIT) in the antibacterial immunity of Marsupenaeus japonicus.
abstract: The Toll/Toll-like receptor (TLR) signaling pathway has an important role in the innate immunity of animals. Evolutionarily conserved signaling intermediate in Toll pathways (ECSIT) is a protein that functions as an adaptor protein for the Toll/TLR and bone morphogenetic protein signaling pathways. ECSIT is also a key component in the macrophage bactericidal activity of mammals. However, the function of ECSIT in crustaceans remains unclear. In this study, we cloned and identified a functional ECSIT homologue, MjECSIT 1, from kuruma shrimp Marsupenaeus japonicus. The complementary DNA of MjEcsit 1 is 1442 base pairs long, with an open reading frame of 1221 base pairs that encodes a 407-residue polypeptide. Transcripts of MjEcsit 1 are detected in hemocytes, gills, hepatopancreas, stomach, heart, intestines, testes, and ovaries. Such transcripts are upregulated by Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Vibrio anguillarum) injections. The knockdown of MjEcsit 1 by double-stranded RNA injection increases the sensitivity of M. japonicus to S. aureus challenge and weakens the bacterial clearance ability of M. japonicus in vivo. In addition, suppressing MjEcsit 1 restrains the upregulation of two anti-lipopolysaccharide factors by S. aureus injection. The results indicate that MjECSIT 1 is important in the antibacterial immunity of M. japonicus.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education, School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Ding
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education, School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Chen
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education, School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Kang
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education, School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China.
authors.forename: Hai-Shan
authors.initials: HS
authors.lastname: Jiang
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education, School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China. Electronic address: cjkang@sdu.edu.cn.
authors.forename: Cui-Jie
authors.initials: CJ
authors.lastname: Kang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D017124:Conserved Sequence; D005786:Gene Expression Regulation / Q000276:immunology; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D009928:Organ Specificity; D033561:Penaeidae / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D051193:Toll-Like Receptors / Q000502:physiology; D014158:Transcription, Genetic; D014733:Vibrio / Q000276:immunology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D051193:Toll-Like Receptors
keywords: Anti-lipopolysaccharide factor (ALF); ECSIT; Innate immunity; Shrimp; Toll/Toll like receptor (TLR) signaling pathway