title: Development of a high-performance liquid chromatography-tandem mass spectrometry method for the identification and quantification of CP-47,497, CP-47,497-C8 and JWH-250 in mouse brain.
abstract: While Marijuana continues to be the most widely used illicit drug, abuse of synthetic cannabinoid (SCB) compounds in 'Spice' or 'K2' herbal incense products has emerged as a significant public health concern in many European countries and in the USA. Several of these SCBs have been declared Schedule I controlled substances but detection and quantification in biological samples remain a challenge. Therefore, we present a liquid chromatography-tandem mass spectrometry method after liquid-liquid extraction for the quantitation of CP-47,497, CP-47,497-C8 and JWH-250 in mouse brain. We report data for linearity, limit of quantification, accuracy/bias, precision, recovery, selectivity, carryover, matrix effects and stability experiments which were developed and fully validated based on Scientific Working Group for Forensic Toxicology guidelines for forensic toxicology method validation. Acceptable coefficients of variation for accuracy/bias, within- and between-run precision and selectivity were determined, with all values within ±15% of the target concentration. Validation experiments revealed degradation of CP-47, 497 and CP-47,497-C8 at different temperatures, and significant ion suppression was produced in brain for all compounds tested. The method was successfully applied to detect and quantify CP-47,497 in brains from mice demonstrating significant cannabimimetic behavioral effects as assessed by the classical tetrad paradigm.
authors.affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University, Box 980613, Richmond, VA 23298-0613, USA samanokl@vcu.edu.
authors.forename: Kimberly L
authors.initials: KL
authors.lastname: Samano
authors.affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University, Box 980613, Richmond, VA 23298-0613, USA.
authors.forename: Justin L
authors.initials: JL
authors.lastname: Poklis
authors.affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University, Box 980613, Richmond, VA 23298-0613, USA.
authors.forename: Aron H
authors.initials: AH
authors.lastname: Lichtman
authors.affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University, Box 980613, Richmond, VA 23298-0613, USA Department of Forensic Science, Virginia Commonwealth University, Richmond, VA, USA Department of Pathology, Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Alphonse
authors.initials: A
authors.lastname: Poklis
mesh_terms: D000818:Animals; D000840:Anisoles / Q000032:analysis* / Q000493:pharmacokinetics / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D002138:Calibration; D002186:Cannabinoids / Q000032:analysis* / Q000493:pharmacokinetics / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003511:Cyclohexanols / Q000032:analysis* / Q000493:pharmacokinetics / Q000494:pharmacology; D007211:Indoles / Q000032:analysis* / Q000493:pharmacokinetics / Q000494:pharmacology; D057230:Limit of Detection; D008297:Male; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D010636:Phenols / Q000032:analysis* / Q000493:pharmacokinetics / Q000494:pharmacology; D012015:Reference Standards; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D014018:Tissue Distribution
chemical_list: C569194:1-(1- pentyl-1H-indol-3-yl)-2-(2-methoxyphenyl)ethanone; C572491:3-(2-hydroxy-4-(2-methylnonan-2-yl)phenyl)cyclohexan-1-ol; D000840:Anisoles; D002186:Cannabinoids; D003511:Cyclohexanols; D007211:Indoles; D010636:Phenols; C036088:CP 47497