title: Fungal lysis by a soil bacterium fermenting cellulose.
abstract: Recycling of plant biomass by a community of bacteria and fungi is fundamental to carbon flow in terrestrial ecosystems. Here we report how the plant fermenting, soil bacterium Clostridium phytofermentans enhances growth on cellulose by simultaneously lysing and consuming model fungi from soil. We investigate the mechanism of fungal lysis to show that among the dozens of different glycoside hydrolases C. phytofermentans secretes on cellulose, the most highly expressed enzymes degrade fungi rather than plant substrates. These enzymes, the GH18 Cphy1799 and Cphy1800, synergize to hydrolyse chitin, a main component of the fungal cell wall. Purified enzymes inhibit fungal growth and mutants lacking either GH18 grow normally on cellulose and other plant substrates, but have a reduced ability to hydrolyse chitinous substrates and fungal hyphae. Thus, C. phytofermentans boosts growth on cellulose by lysing fungi with its most highly expressed hydrolases, highlighting the importance of fungal interactions to the ecology of cellulolytic bacteria.
authors.affiliation: CEA, DSV, IG, Genoscope, Évry, France.|CNRS-UMR8030, Évry, France.|Université d'Évry Val d'Essonne, Évry, France.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Tolonen
authors.affiliation: CEA, DSV, IG, Genoscope, Évry, France.|CNRS-UMR8030, Évry, France.|Université d'Évry Val d'Essonne, Évry, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Cerisy
authors.affiliation: CEA, DSV, IG, Genoscope, Évry, France.|CNRS-UMR8030, Évry, France.|Université d'Évry Val d'Essonne, Évry, France.
authors.forename: Hafez
authors.initials: H
authors.lastname: El-Sayyed
authors.affiliation: CEA, DSV, IG, Genoscope, Évry, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Boutard
authors.affiliation: CEA, DSV, IG, Genoscope, Évry, France.|CNRS-UMR8030, Évry, France.|Université d'Évry Val d'Essonne, Évry, France.
authors.forename: Marcel
authors.initials: M
authors.lastname: Salanoubat
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA, USA.
authors.forename: George M
authors.initials: GM
authors.lastname: Church
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism*; D003013:Clostridium / Q000201:enzymology* / Q000254:growth & development*; D017753:Ecosystem; D005285:Fermentation; D005658:Fungi / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D010944:Plants / Q000378:metabolism; D012987:Soil; D012988:Soil Microbiology
chemical_list: D012987:Soil; D002686:Chitin; D002482:Cellulose; D006026:Glycoside Hydrolases