title: Intranasal immunisation of the recombinant Toxoplasma gondii receptor for activated C kinase 1 partly protects mice against T. gondii infection.
abstract: Nasal vaccination is an effective therapeutic regimen for preventing certain infectious diseases. The mucosal immune response is important for resistance to Toxoplasma gondii infection. In this study, we evaluated the immune responses elicited in BALB/c mice by nasal immunisation with recombinant T. gondii receptor for activated C kinase 1 (rTgRACK1) and their protective efficacy against T. gondii RH strain during both chronic and lethal infections. Nasal vaccination with rTgRACK1 increased the level of secretory IgA in nasal, intestinal and vesical washes, and the level of IFN-γ and IL-2 in intestinal washes, indicating that rTgRACK1 vaccination promotes mucosal immune responses. The mice immunised with rTgRACK1 also displayed increased levels of rTgRACK1-specific IgA, total IgG, IgG1 and in particular IgG2a in their blood sera, increased production of IFN-γ, IL-2 and IL-4 but not IL-10 from their isolated spleen cells, and enhanced splenocyte proliferation in vitro. rTgRACK1-vaccinated mice were effectively protected against infection with T. gondii RH strain, showing over 50% reduction of tachyzoite burdens in their liver and brain tissues during a chronic infection, and also a 45% increase in their survivals during a lethal challenge. These results indicate that rTgRACK1 might represent an intriguing immunogen for developing a mucosal vaccine against toxoplasmosis.
authors.affiliation: Research Institute of Medical Parasitology, Shanxi Medical University, Taiyuan, Shanxi 030001, China. Electronic address: longwty.syd@gmail.com.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Wang
authors.affiliation: Department of Respiratory, The First Affiliated Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
authors.forename: Min
authors.initials: M
authors.lastname: Pang
authors.affiliation: Department of Physiology, Key Laboratory of Cellular Physiology Co-constructed by Province and Ministry of Education, Shanxi Medical University, Taiyuan, China.
authors.forename: Li-Tian
authors.initials: LT
authors.lastname: Yin
authors.affiliation: Research Institute of Medical Parasitology, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Zhang
authors.affiliation: Research Institute of Medical Parasitology, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Meng
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Yu
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: Department of Physiology, Faculty of Medical and Health Sciences, University of Auckland, Private bag 92-019, Auckland 1142, New Zealand.
authors.forename: Ji-Zhong
authors.initials: JZ
authors.lastname: Bai
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi 030001, China; Centre for Transplantation and Renal Research, the University of Sydney at Westmead Millennium Institute, Sydney, NSW 2145 Australia.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zheng
authors.affiliation: Research Institute of Medical Parasitology, Shanxi Medical University, Taiyuan, Shanxi 030001, China. Electronic address: guorongyin@163.com.
authors.forename: Guo-Rong
authors.initials: GR
authors.lastname: Yin
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D018928:Immunity, Mucosal; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008807:Mice, Inbred BALB C; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000276:immunology; D016019:Survival Analysis; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D016207:Cytokines; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D016052:Protozoan Vaccines; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic
keywords: Intranasal immunisation; Mucosal immune response; Receptor for activated C kinase 1; Recombinant protein; Secretory IgA; Toxoplasmosis