title: Rosenbergiella australoborealis sp. nov., Rosenbergiella collisarenosi sp. nov. and Rosenbergiella epipactidis sp. nov., three novel bacterial species isolated from floral nectar.
abstract: The taxonomic status of nine strains of the family Enterobacteriaceae isolated from floral nectar of wild Belgian, French, South African and Spanish insect-pollinated plants was investigated following a polyphasic approach. Confirmation that these strains belonged to the genus Rosenbergiella was obtained from comparative analysis of partial sequences of the 16S rRNA gene and other core housekeeping genes (atpD [ATP synthase β-chain], gyrB [DNA gyrase subunit B] and rpoB [RNA polymerase β-subunit]), DNA-DNA reassociation data, determination of the DNA G+C content and phenotypic profiling. Two strains belonged to the recently described species Rosenbergiella nectarea, while the other seven strains represented three novel species within the genus Rosenbergiella. The names Rosenbergiella australoborealis sp. nov. (with strain CdVSA 20.1(T) [LMG 27954(T)=CECT 8500(T)] as the type strain), Rosenbergiella collisarenosi sp. nov. (with strain 8.8A(T) [LMG 27955(T)=CECT 8501(T)] as the type strain) and Rosenbergiella epipactidis sp. nov. (with strain 2.1A(T) [LMG 27956(T)=CECT 8502(T)] as the type strain) are proposed. Additionally, the description of the genus Rosenbergiella is updated on the basis of new phenotypic and molecular data.
authors.affiliation: Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), Cluster for Bioengineering Technology (CBet), Department of Microbial and Molecular Systems (M(2)S), KU Leuven, Campus De Nayer, B-2860 Sint-Katelijne-Waver, Belgium.
authors.forename: Marijke
authors.initials: M
authors.lastname: Lenaerts
authors.affiliation: Department of Animal Health, Faculty of Veterinary Medicine, Universidad Complutense de Madrid, E-28040 Madrid, Spain. Electronic address: sealperez@gmail.com.
authors.forename: Sergio
authors.initials: S
authors.lastname: Alvarez-Pérez
authors.affiliation: Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), E-41092 Sevilla, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: de Vega
authors.affiliation: Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), Cluster for Bioengineering Technology (CBet), Department of Microbial and Molecular Systems (M(2)S), KU Leuven, Campus De Nayer, B-2860 Sint-Katelijne-Waver, Belgium.
authors.forename: Ado
authors.initials: A
authors.lastname: Van Assche
authors.affiliation: School of Life Sciences, University of KwaZulu-Natal, Scottsville, Pietermaritzburg 3209, South Africa.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Johnson
authors.affiliation: Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), Cluster for Bioengineering Technology (CBet), Department of Microbial and Molecular Systems (M(2)S), KU Leuven, Campus De Nayer, B-2860 Sint-Katelijne-Waver, Belgium.
authors.forename: Kris A
authors.initials: KA
authors.lastname: Willems
authors.affiliation: Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), E-41092 Sevilla, Spain.
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Herrera
authors.affiliation: Division of Plant Ecology and Systematics, Biology Department, KU Leuven, B-3001 Heverlee, Belgium.
authors.forename: Hans
authors.initials: H
authors.lastname: Jacquemyn
authors.affiliation: Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), Cluster for Bioengineering Technology (CBet), Department of Microbial and Molecular Systems (M(2)S), KU Leuven, Campus De Nayer, B-2860 Sint-Katelijne-Waver, Belgium. Electronic address: bart.lievens@kuleuven.be.
authors.forename: Bart
authors.initials: B
authors.lastname: Lievens
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D020043:Genes, Essential; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D057048:Plant Nectar; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D057048:Plant Nectar; D012336:RNA, Ribosomal, 16S
keywords: Enterobacteriaceae; Nectar; Rosenbergiella