title: Whole Body Vibration Reduces Inflammatory Bone Loss in a Lipopolysaccharide Murine Model.
abstract: Whole body vibration (WBV) stimulation has a beneficial effect on the recovery of osteoporotic bone. We aimed to investigate the immediate effect of WBV on lipopolysaccharide (LPS)-mediated inflammatory bone loss by varying the exposure timing. Balb/C mice were divided into the following groups: control, LPS (L), and LPS with vibration (LV). The L and LV groups received LPS (5 mg/kg) by 2 intraperitoneal injections on days 0 and 4. The LV group was exposed to WBV (0.4 g, 45 Hz) either during LPS treatment (LV1) or after cessation of LPS injection (LV2) and then continued WBV treatment for 10 min/d for 3 d. Evaluation based on micro-computed tomography was performed 7 d after the first injection, when the L group showed a significant decrease in bone volume (-25.8%) and bone mineral density (-33.5%) compared with the control group. The LV2 group recovered bone volume (35%) and bone mineral density (19.9%) compared with the L group, whereas the LV1 group showed no improvement. This vibratory signal showed a suppressive effect on the LPS-mediated induction of inflammatory cytokines such as IL-1β or TNF-α in human mesenchymal stem cells in vitro. These findings suggest that immediate exposure to WBV after the conclusion of LPS treatment efficiently reduces trabecular bone loss, but WBV might be less effective during the course of treatment with inflammatory factor.
authors.affiliation: Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: I S
authors.initials: IS
authors.lastname: Kim
authors.affiliation: Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: B
authors.initials: B
authors.lastname: Lee
authors.affiliation: Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: S J
authors.initials: SJ
authors.lastname: Yoo
authors.affiliation: Dental Research Institute, Seoul National University, Seoul, Republic of Korea Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Republic of Korea sjhwang@snu.ac.kr.
authors.forename: S J
authors.initials: SJ
authors.lastname: Hwang
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000328:Adult; D000818:Animals; D015519:Bone Density / Q000502:physiology; D001861:Bone Regeneration / Q000502:physiology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005269:Femur / Q000503:physiopathology; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size; D010024:Osteoporosis / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D000071681:Tartrate-Resistant Acid Phosphatase; D013977:Tibia / Q000503:physiopathology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D014732:Vibration / Q000627:therapeutic use*; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D053583:Interleukin-1beta; D007527:Isoenzymes; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: cytokines; inflammation; lipopolysaccharides; recovery; resorption; tibia