title: Exploiting fast detectors to enter a new dimension in room-temperature crystallography.
abstract: A departure from a linear or an exponential intensity decay in the diffracting power of protein crystals as a function of absorbed dose is reported. The observation of a lag phase raises the possibility of collecting significantly more data from crystals held at room temperature before an intolerable intensity decay is reached. A simple model accounting for the form of the intensity decay is reintroduced and is applied for the first time to high frame-rate room-temperature data collection.
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
authors.forename: Robin L
authors.initials: RL
authors.lastname: Owen
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
authors.forename: Neil
authors.initials: N
authors.lastname: Paterson
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
authors.forename: Danny
authors.initials: D
authors.lastname: Axford
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
authors.forename: Jun
authors.initials: J
authors.lastname: Aishima
authors.affiliation: Dectris Ltd, Neuenhofer Strasse 107, 5400 Baden, Switzerland.
authors.forename: Clemens
authors.initials: C
authors.lastname: Schulze-Briese
authors.affiliation: Division of Structural Biology, University of Oxford, The Henry Wellcome Building for Genomic Medicine, Roosevelt Drive, Oxford OX3 7BN, England.
authors.forename: Jingshan
authors.initials: J
authors.lastname: Ren
authors.affiliation: Division of Structural Biology, University of Oxford, The Henry Wellcome Building for Genomic Medicine, Roosevelt Drive, Oxford OX3 7BN, England.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Fry
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
authors.forename: David I
authors.initials: DI
authors.lastname: Stuart
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
authors.forename: Gwyndaf
authors.initials: G
authors.lastname: Evans
mesh_terms: D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D029650:Enterovirus, Bovine / Q000737:chemistry; D029544:Foot-and-Mouth Disease Virus / Q000737:chemistry; D008962:Models, Theoretical; D011506:Proteins / Q000737:chemistry; D013696:Temperature
chemical_list: D011506:Proteins
keywords: dose rate; macromolecular crystallography; radiation damage; room temperature