title: Astragalus polysaccharide ameliorates ionizing radiation-induced oxidative stress in mice.
abstract: Radioprotective compounds from plant resources may represent safe and cost-effective prophylactic and therapeutic agents. This study was designed to investigate the protective effect of polysaccharide derived from the dried roots of the Astragalus spp. (APS) against ionizing radiation (IR) injury in liver and to explore its role in radiation-induced oxidative stress using a mouse model. Prior to (60)Co γ-irradiation (5Gy, single dose), mice received 7 days of APS at low, mid and high doses (50, 100 or 200mg/kg/day, respectively; n=6 each group), vehicle alone (5mL normal saline orally/daily; n=6). A non-irradiated control group (n=6) received the 7-day distilled water regimen only. At 24h post-irradiation, the APS pre-treated mice showed significantly decreased alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase levels, and NF-κB expression. All APS-treated mice also showed attenuation of the IR-induced increase in thiobarbituric acid reactive substance and resolution of the IR-induced decreases in superoxide dismutase, catalase and glutathione activities (all p<0.05). High dose APS pre-treatment led to remarkably less morphologic features of IR-induced hepatic and pulmonary injury. Thus, APS exerts protective effects against IR-induced injury in liver in mice, and the related molecular mechanism may involve suppressing the radiation-induced oxidative stress reaction.
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, 29 Gao Tan Yan Street, Chongqing 400038, Shapingba District, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, 29 Gao Tan Yan Street, Chongqing 400038, Shapingba District, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, 29 Gao Tan Yan Street, Chongqing 400038, Shapingba District, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, 29 Gao Tan Yan Street, Chongqing 400038, Shapingba District, China.
authors.forename: Di
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, 29 Gao Tan Yan Street, Chongqing 400038, Shapingba District, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lv
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, 29 Gao Tan Yan Street, Chongqing 400038, Shapingba District, China.
authors.forename: Yangjin
authors.initials: Y
authors.lastname: Ou
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, 29 Gao Tan Yan Street, Chongqing 400038, Shapingba District, China.
authors.forename: Fengjun
authors.initials: F
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, 29 Gao Tan Yan Street, Chongqing 400038, Shapingba District, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, 29 Gao Tan Yan Street, Chongqing 400038, Shapingba District, China. Electronic address: swhshiy@tom.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, 29 Gao Tan Yan Street, Chongqing 400038, Shapingba District, China. Electronic address: py_xia2013@163.com.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Xia
mesh_terms: D000818:Animals; D027883:Astragalus Plant / Q000737:chemistry*; D015415:Biomarkers / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D011134:Polysaccharides / Q000494:pharmacology*; D011839:Radiation, Ionizing; D017550:Spectroscopy, Fourier Transform Infrared; D014916:Whole-Body Irradiation
chemical_list: D015415:Biomarkers; D009005:Monosaccharides; D016328:NF-kappa B; D011134:Polysaccharides
keywords: Astragalus polysaccharide; Oxidative stress; Radiation