title: Protective effects of minocycline against short-term ischemia-reperfusion injury in rat brain.
abstract: The aim of this study was to assess the effects of minocycline on cerebral ischemia-reperfusion (I/R) injury in rats. The study was carried out on 24 male Wistar albino rats, weighing 200-250 g, which were divided into three groups: (i) control (n = 8), (ii) I/R (n = 8) and (iii) I/R + minocycline (n = 8). Minocycline was administrated at a dose of 90 mg/kg p.o. to the I/R group 48, 24 and 1 h before ischemia. Following bilateral exposure of the common carotid arteries by anterior cervical dissection and separation of the vagus nerve, I/R injury was performed by occlusion. Following reperfusion, malondialdehyde (MDA), superoxide dismutase, glutathione peroxidase and catalase levels in the blood and brain tissue, and creatine kinase (CK), CK-BB, lactate dehydrogenase (LDH), neuron-specific enolase (NSE) and protein S100β levels in the blood were measured and the histopathological changes were monitored. Regarding histopathological evaluation, symptoms of degeneration were significantly improved in the I/R + minocycline group compared to the I/R-only group. Statistical analysis of the biochemical parameters revealed significant differences in MDA (p < 0.001), nitric oxide (p < 0.05), CK (p < 0.05) and CK-MB (p < 0.05) levels between the I/R + minocycline group and the I/R group. According to the literature, the effect of minocycline is firstly assessed by LDH, CK-MB, NSE and S-100β analysis in addition to antioxidant status and histopathological analysis.
authors.affiliation: Department of Neurosurgery, Tayfur Ata Sökmen Medical Faculty, Mustafa Kemal University, Hatay, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Aras
authors.forename: Boran
authors.initials: B
authors.lastname: Urfalı
authors.forename: Yurdal
authors.initials: Y
authors.lastname: Serarslan
authors.forename: Tümay
authors.initials: T
authors.lastname: Ozgür
authors.forename: Kemal Türker
authors.initials: KT
authors.lastname: Ulutaş
authors.forename: Senem
authors.initials: S
authors.lastname: Urfalı
authors.forename: Murat
authors.initials: M
authors.lastname: Altaş
authors.forename: Nebi
authors.initials: N
authors.lastname: Yılmaz
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D008911:Minocycline / Q000494:pharmacology / Q000627:therapeutic use*; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D018696:Neuroprotective Agents; D008911:Minocycline