title: Anti-proteolytic capacity and bonding durability of proanthocyanidin-biomodified demineralized dentin matrix.
abstract: Our previous studies showed that biomodification of demineralized dentin collagen with proanthocyanidin (PA) for a clinically practical duration improves the mechanical properties of the dentin matrix and the immediate resin-dentin bond strength. The present study sought to evaluate the ability of PA biomodification to reduce collagenase-induced biodegradation of demineralized dentin matrix and dentin/adhesive interfaces in a clinically relevant manner. The effects of collagenolytic and gelatinolytic activity on PA-biomodified demineralized dentin matrix were analysed by hydroxyproline assay and gelatin zymography. Then, resin-/dentin-bonded specimens were prepared and challenged with bacterial collagenases. Dentin treated with 2% chlorhexidine and untreated dentin were used as a positive and negative control, respectively. Collagen biodegradation, the microtensile bond strengths of bonded specimens and the micromorphologies of the fractured interfaces were assessed. The results revealed that both collagenolytic and gelatinolytic activity on demineralized dentin were notably inhibited in the PA-biomodified groups, irrespective of PA concentration and biomodification duration. When challenged with exogenous collagenases, PA-biomodified bonded specimens exhibited significantly less biodegradation and maintained higher bond strengths than the untreated control. These results suggest that PA biomodification was effective at inhibiting proteolytic activity on demineralized dentin matrix and at stabilizing the adhesive/dentin interface against enzymatic degradation, is a new concept that has the potential to improve bonding durability.
authors.affiliation: 1] State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China [2] Department of Prosthodontics, Stomatology Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, China.
authors.forename: Rui-Rui
authors.initials: RR
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
authors.forename: Cheng-Fang
authors.initials: CF
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Dou
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Chen
mesh_terms: D002710:Chlorhexidine / Q000737:chemistry / Q000494:pharmacology; D017364:Collagenases / Q000494:pharmacology; D001840:Dental Bonding; D003738:Dental Cements / Q000737:chemistry; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D018093:Gelatinases / Q000494:pharmacology; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis; D020784:Matrix Metalloproteinase 8 / Q000494:pharmacology; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000737:chemistry / Q000494:pharmacology*; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D017001:Tooth Demineralization / Q000473:pathology / Q000503:physiopathology*
chemical_list: C554641:Adper single bond 2; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D061965:Matrix Metalloproteinase Inhibitors; D044945:Proanthocyanidins; C013221:proanthocyanidin; D017364:Collagenases; D018093:Gelatinases; C417249:collagenase 1; D020784:Matrix Metalloproteinase 8; D002710:Chlorhexidine; D006909:Hydroxyproline