title: Biosensors for qualitative and semiquantitative analysis of quorum sensing signal molecules.
abstract: Biosensors are biological tools that can be used to assay bacterial cultures for quorum sensing signal molecules (QSSMs) both qualitatively and semiquantitatively. QSSMs can be extracted from Pseudomonas aeruginosa cultures using organic solvents and tentatively identified via thin layer chromatography in combination with biosensor overlays. Alternatively, QSSMs can be quantified in spent culture supernatants or solvent extracts using biosensor-based spectrophotometric, luminescence, or fluorescence assays.
authors.affiliation: School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
authors.forename: Matthew
authors.initials: M
authors.lastname: Fletcher
authors.forename: Miguel
authors.initials: M
authors.lastname: Cámara
authors.forename: David A
authors.initials: DA
authors.lastname: Barrett
authors.forename: Paul
authors.initials: P
authors.lastname: Williams
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002855:Chromatography, Thin Layer; D008163:Luminescent Measurements; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D015363:Quinolones / Q000737:chemistry / Q000302:isolation & purification; D053038:Quorum Sensing; D015398:Signal Transduction; D013053:Spectrophotometry
chemical_list: D015363:Quinolones