title: Fluorescence properties of selected benzo[c]phenanthridines.
abstract: The fluorescence properties of selected benzo[c]phenanthridines (BPs) were examined. The effect of structure, pH and solvent on the fluorescence properties has been investigated. It was found out that the presence of charged iminium nitrogen significantly decreased the fluorescence of the compounds. The fluorescence (intensity as well as emission spectra shape) of the investigated compounds was significantly dependent on pH as well as used solvent. The utilization in epigenetic modification mechanisms studies as demethylase probe and as possible pH indicator was suggested.
authors.affiliation: Department of Organic Chemistry, Faculty of Science, Institute of Molecular and Translational Medicine, Palacký University, 17. Listopadu 12, 771 46, Olomouc, Czech Republic, motyka@orgchem.upol.cz.
authors.forename: Kamil
authors.initials: K
authors.lastname: Motyka
authors.forename: Jakub
authors.initials: J
authors.lastname: Stýskala
authors.forename: Petr
authors.initials: P
authors.lastname: Cankař
authors.forename: Jan
authors.initials: J
authors.lastname: Hlaváč
mesh_terms: D053119:Benzophenanthridines / Q000138:chemical synthesis / Q000737:chemistry*; D005434:Flow Cytometry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D053119:Benzophenanthridines; D005456:Fluorescent Dyes; D012997:Solvents