title: Changes in memory and synaptic plasticity induced in male rats after maternal exposure to bisphenol A.
abstract: Bisphenol A (BPA), a component of polycarbonate and epoxy resins, has been reported to adversely impact the central nervous system, especially with respect to learning and memory. However, the precise effect and specific mechanisms have not been fully elucidated. In the present study, pregnant Sprague-Dawley rats were orally administered with BPA at 0.05, 0.5, 5 or 50mg/kg·body weight (BW) per day from embryonic day 9 (E 9) to E 20. We examined the effects of maternal BPA exposure on memory and synaptic structure in the hippocampus of male offspring at postnatal day (PND) 21. Maternal BPA exposure significantly affected locomotor activity, exploratory habits, and emotional behavior in open field test, and increased reference and especially working memory errors in the radial arm maze during the postnatal developing stage. Maternal BPA exposure had an adverse effect on synaptic structure, including a widened synaptic cleft, a thinned postsynaptic density (PSD), unclear synaptic surface and disappeared synaptic vesicles. Furthermore, maternal BPA exposure decreased the mRNA and protein expressions of synaptophysin, PSD-95, spinophilin, GluR1 and NMDAR1 in the hippocampus of male offspring on PND 21. These results showed that fetal growth and development was more sensitive to BPA exposure. The decreased learning and memory induced by maternal exposure to BPA in this study may be involved in synaptic plasticity alteration.
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, PR China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, PR China.
authors.forename: Ruiyan
authors.initials: R
authors.lastname: Niu
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, PR China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, PR China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Han
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, PR China.
authors.forename: Guangying
authors.initials: G
authors.lastname: Luo
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, PR China.
authors.forename: Bingrui
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, PR China. Electronic address: wangjd53@outlook.com.
authors.forename: Jundong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001559:Benzhydryl Compounds / Q000633:toxicity*; D015153:Blotting, Western; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects; D008431:Maternal-Fetal Exchange; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects*; D046529:Microscopy, Electron, Transmission; D009043:Motor Activity / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D009473:Neuronal Plasticity / Q000187:drug effects*; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013569:Synapses / Q000187:drug effects* / Q000648:ultrastructure; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D012739:Gonadal Steroid Hormones; D009419:Nerve Tissue Proteins; D010636:Phenols; D012313:RNA; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Glutamate receptors; Learning and memory; Synaptic marker; Synaptic structural