title: Brain quantitative proteomics combining GeLC-MS and isotope-coded protein labeling (ICPL).
abstract: Proteomics has been revolutionized by the rapid advance of mass spectrometric instrumentations and techniques. Parallel methodologies for the quantification of proteomes also evolved, including in vitro stable isotope labeling. Here, we present a protocol for employing isotope-coded protein labeling (ICPL) as part of a shotgun proteomics workflow denoting its advantages and disadvantages. This protocol is suitable to studying any proteome of interest, only requiring a specific sample preparation and protein identification. Given our expertise, descriptions here are centered on the study of brain disorders.
authors.affiliation: Proteomics and Biomarkers, Max Planck Institute of Psychiatry, Kraepelinstr. 2, Munich, 80804, Germany.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Maccarrone
authors.forename: Maria
authors.initials: M
authors.lastname: Lebar
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
mesh_terms: D001921:Brain / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007553:Isotope Labeling; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009419:Nerve Tissue Proteins