title: Quantitative detection of epidermal growth factor and interleukin-8 in whole saliva of healthy individuals.
abstract: OBJECTIVE
  This study aims to create consensus concerning the use of a methodology by which the handling of saliva is standardized and quantitative detection of IL-8 and EGF in whole saliva is achieved. Our study involves evaluating the extent to which the pre-treatment of saliva samples with an anionic detergent - sodium dodecyl sulphate (SDS) - improved detection levels for IL-8 and EGF.
  
  
  METHODS
  Whole saliva samples (n=28) were collected from healthy individuals and a protease inhibitor cocktail was added immediately. They were treated with either SDS or PBS for 20min and were then applied to a sandwich ELISA.
  
  
  CONCLUSIONS
  Saliva is a complex viscous fluid that requires degrading before the analysis of salivary biomarkers. We found that pre-treatment of samples with SDS significantly increased the detection levels for both EGF (293%) and IL-8 (346%) when compared with PBS-treated pairs (***P<0.001). According to the results we recommend: (i) pre-treatment of whole saliva samples with SDS for quantitative analysis (ii) using secretory output instead of concentration in the presentation of results to avoid individual variations and (iii) taking into consideration gender, age and meal intake since these have an impact on the secretory output of salivary proteins.
authors.affiliation: Department of Oral Medicine and Pathology, University of Gothenburg, Odontology Institute at Sahlgrenska Academy, Gothenburg, Sweden.
authors.forename: Amal
authors.initials: A
authors.lastname: Dafar
authors.affiliation: Department of Oral Medicine and Pathology, University of Gothenburg, Odontology Institute at Sahlgrenska Academy, Gothenburg, Sweden.
authors.forename: Paula
authors.initials: P
authors.lastname: Rico
authors.affiliation: Department of Oral Medicine and Pathology, University of Gothenburg, Odontology Institute at Sahlgrenska Academy, Gothenburg, Sweden.
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Işık
authors.affiliation: Department of Oral Medicine and Pathology, University of Gothenburg, Odontology Institute at Sahlgrenska Academy, Gothenburg, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Jontell
authors.affiliation: Department of Oral Medicine and Pathology, University of Gothenburg, Odontology Institute at Sahlgrenska Academy, Gothenburg, Sweden. Electronic address: hulya.cevik@odontologi.gu.se.
authors.forename: Hülya
authors.initials: H
authors.lastname: Cevik-Aras
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D003902:Detergents / Q000737:chemistry; D004435:Eating; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D004815:Epidermal Growth Factor / Q000032:analysis*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011480:Protease Inhibitors / Q000737:chemistry; D012463:Saliva / Q000737:chemistry*; D012472:Salivation; D012737:Sex Factors; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013048:Specimen Handling; D055815:Young Adult
chemical_list: D015415:Biomarkers; C508606:CXCL8 protein, human; D003902:Detergents; D016209:Interleukin-8; D011480:Protease Inhibitors; D012967:Sodium Dodecyl Sulfate; D004815:Epidermal Growth Factor
keywords: ELISA; Oral diseases; Protein biomarkers; SDS; Salivary diagnostics