title: Expression patterns of metallothionein, cytochrome P450 1A and vitellogenin genes in western mosquitofish (Gambusia affinis) in response to heavy metals.
abstract: The aim of this study was to evaluate the effects of three metals (Zn, Cd and Pb) on hepatic metallothionein (MT), cytochrome P450 1A (CYP1A) and vitellogenin (Vtg) mRNA expression in the liver of adult female mosquitofish (Gambusia affinis) after 1, 3 or 8d. Both concentration-response and time-course effects of hepatic MT, CYP1A and Vtg at the transcription level were determined by quantitative real-time PCR. The results from this study showed that Zn, Cd and Pb could significantly induced MT, CYP1A and Vtg mRNA expression levels in mosquitofish. In general, this study demonstrated that heavy metals modulate MT, CYP1A and Vtg mRNA expression levels in a metal-, concentration- or time-dependent manner.
authors.affiliation: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: huang_gyh@sina.com.
authors.forename: Guo-Yong
authors.initials: GY
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: guangguo.ying@gmail.com.
authors.forename: Guang-Guo
authors.initials: GG
authors.lastname: Ying
authors.affiliation: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Yan-Qiu
authors.initials: YQ
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: You-Sheng
authors.initials: YS
authors.lastname: Liu
mesh_terms: D000818:Animals; D003532:Cyprinodontiformes / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects; D008297:Male; D008668:Metallothionein / Q000235:genetics; D019216:Metals, Heavy / Q000633:toxicity*; D014819:Vitellogenins / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D019216:Metals, Heavy; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D008668:Metallothionein
keywords: Cytochrome P450 1A; Heavy metals; Metallothionein; Mosquitofish; Vitellogenin