title: Transcriptional analysis of ORF amv133 of Amsacta moorei entomopoxvirus.
abstract: The open reading frame (ORF) amv133 of Amsacta moorei entomopoxvirus, encodes a putative lipase gene. Its temporal expression pattern was characterized by RT-PCR and found to start at 6 h postinfection (h p.i.) and reach a maximum level at 48 h p.i. While the ORF has a late promoter motif, the inhibition of viral DNA synthesis by Ara-C failed to inhibit transcription, but a general inhibitor of protein synthesis prevented its transcription, indicating that amv133 is an intermediate gene. 5'-RACE analysis showed that transcription was initiated at position -77 relative to the translational start site. To determine the size of the promoter, several truncations were generated and cloned upstream of the firefly luciferase reporter gene. The resulting constructs were tested in a dual assay. A fragment that contained 115 bp relative to the transcription start site exhibited optimum promoter length.
authors.affiliation: Department of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Ozsahin
authors.forename: Kazım
authors.initials: K
authors.lastname: Sezen
authors.forename: Zihni
authors.initials: Z
authors.lastname: Demirbag
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D002460:Cell Line; D003561:Cytarabine / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000235:genetics; D017996:Entomopoxvirinae / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D008049:Lipase / Q000235:genetics*; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology; D016366:Open Reading Frames / Q000235:genetics*; D011213:Poxviridae Infections; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis / Q000187:drug effects; D024363:Transcription Initiation Site; D014158:Transcription, Genetic / Q000187:drug effects; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D000998:Antiviral Agents; D004279:DNA, Viral; D014764:Viral Proteins; D003561:Cytarabine; D008049:Lipase