title: Behavior of encapsulated MG-63 cells in RGD and gelatine-modified alginate hydrogels.
abstract: Achieving cell spreading and proliferation inside hydrogels that are compatible with microencapsulation technology represents a major challenge for tissue engineering scaffolding and for the development of three-dimensional cell culture models. In this study, microcapsules of 650-900 μm in diameter were fabricated from oxidized alginate covalently cross-linked with gelatine (AlGel). Schiff's base bond formed in AlGel, detected by Fourier transform infrared spectroscopy, which confirmed the cross-linking of oxidized alginate with gelatine. Biological properties of alginate based hydrogels were studied by comparing the viability and morphology of MG-63 osteosarcoma cells encapsulated in gelatine and RGD-modified alginate. We hypothesized that the presence of gelatine and RGD will support cell adhesion and spreading inside the microcapsules and finally, also vascular endothelial growth factor (VEGF) secretion. After 4 days of incubation, cells formed extensive cortical protrusions and after 2 weeks they proliferated, migrated, and formed cellular networks through the AlGel material. In contrast, cells encapsulated in pure alginate and in RGD-modified alginate formed spherical aggregates with limited cell mobility and VEGF secretion. Metabolic activity was doubled after 5 days of incubation, making AlGel a promising material for cell encapsulation.
authors.affiliation: 1 Institute of Biomaterials, University of Erlangen-Nuremberg , Erlangen, Germany .
authors.forename: Alexandra
authors.initials: A
authors.lastname: Grigore
authors.forename: Bapi
authors.initials: B
authors.lastname: Sarker
authors.forename: Ben
authors.initials: B
authors.lastname: Fabry
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
authors.forename: Rainer
authors.initials: R
authors.lastname: Detsch
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D055119:Elastic Modulus / Q000187:drug effects; D005780:Gelatin / Q000494:pharmacology*; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D020100:Hydrogels / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D034421:Sus scrofa; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; D009842:Oligopeptides; D042461:Vascular Endothelial Growth Factor A; C047981:arginyl-glycyl-aspartic acid; D020723:Glucuronic Acid; D005780:Gelatin