title: Fluorescence imaging of MACPF/CDC proteins: new techniques and their application.
abstract: Structural and biochemical investigations have helped illuminate many of the important details of MACPF/CDC pore formation. However, conventional techniques are limited in their ability to tackle many of the remaining key questions, and new biophysical techniques might provide the means to improve our understanding. Here we attempt to identify the properties of MACPF/CDC proteins that warrant further study, and explore how new developments in fluorescence imaging are able to probe these properties.
authors.affiliation: Department of Chemistry, Oxford University, 12 Mansfield Rd, Oxford, OX1 3TA, UK.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Senior
authors.forename: Mark I
authors.initials: MI
authors.lastname: Wallace
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism; D015938:Complement Membrane Attack Complex / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D054353:Perforin / Q000737:chemistry* / Q000378:metabolism; D052899:Pore Forming Cytotoxic Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D015938:Complement Membrane Attack Complex; D008051:Lipid Bilayers; D052899:Pore Forming Cytotoxic Proteins; D054353:Perforin; D002784:Cholesterol