title: Development of liposomal nanoconstructs targeting P-selectin (CD62P)-expressing cells by using a sulfated derivative of sialic acid.
abstract: OBJECTIVE
  NMSO3, a sulfated derivative of sialic acid, is a specific inhibitor for P-selectin (CD62P)-mediated cell adhesion. We attempted to apply liposomes modified with NMSO3 for selective targeting of activated platelets.
  
  
  METHODS
  The binding of fluorescently labeled NMSO3-containing liposomes (NMSO3-liposomes) to CHO cells expressing P-selectin (CHO-P cells) and activated platelets were examined. The distribution of NMSO3-liposomes incorporated into the cells was observed by fluorescence microscopy.
  
  
  RESULTS
  The binding assay revealed that NMSO3-liposomes specifically bound to immobilized P-selectin and CHO-P cells in a dose-dependent manner. The binding of NMSO3-liposomes to CHO-P cells was much stronger than that to the parental CHO-K1 cells. Fluorescence microscopic observation showed that NMSO3-liposomes were incorporated into CHO-P cells after the binding and distributed throughout the cytoplasm of the cell. NMSO3-liposomes bound more strongly to thrombin-activated platelets than to resting platelets, as assessed by flow cytometry.
  
  
  CONCLUSIONS
  These results suggest that NMSO3-liposomes can be applied for selective drug delivery to activated platelets.
authors.affiliation: Department of Microbiology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa-ku, Tokyo, 142-8501, Japan.
authors.forename: Saotomo
authors.initials: S
authors.lastname: Itoh
authors.forename: Kumi
authors.initials: K
authors.lastname: Kawano
authors.forename: Kana
authors.initials: K
authors.lastname: Takeshita
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Maitani
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D016466:CHO Cells; D002448:Cell Adhesion / Q000187:drug effects; D003412:Cricetulus; D004337:Drug Carriers / Q000737:chemistry*; D005434:Flow Cytometry; D006801:Humans; D008055:Lipids / Q000008:administration & dosage* / Q000494:pharmacology; D008081:Liposomes; D019158:N-Acetylneuraminic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D049329:Nanostructures / Q000737:chemistry*; D019007:P-Selectin / Q000235:genetics / Q000378:metabolism*; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects*; D014162:Transfection
chemical_list: C413863:(2,2-bis(docosyloxymethyl)propyl-5-acetamido-3,5-dideoxy-4,7,8,9-tetra-O-(sodium oxysulfonyl)glycero-alpha-galacto-2-nonulopyranosid)onate; D004337:Drug Carriers; D008055:Lipids; D008081:Liposomes; D019007:P-Selectin; D019158:N-Acetylneuraminic Acid