title: MiR-29b protects dorsal root ganglia neurons from diabetic rat.
abstract: Accumulated evidences implicated that microRNAs may be involved in diabetic neuropathy. Here, we investigated miR-29's roles in primary isolated dorsal root ganglion (DRG) neurons from STZ-induced diabetic rats. First, miR-29b was found down-regulated after STZ-injection. Inhibitions were increased with time course. Down-regulation of miR-29b was associated with higher apoptosis rate and more serious axonal swelling. Meanwhile, axonogeneration genes were inhibited, whereas neurodegenerative genes were stimulated. Restoration of miR-29b by mimic experiment could reverse the above neuropathy. Furthermore, western blot analysis disclosed that miR-29b could abolish Smad3 activation. In conclusion, the present study identifies that miR-29b could protect DRG from diabetic rats. This protective effects suggested potential therapeutic application of miR-29b in diabetic neuropathy.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Jilin University, 71 Xinmin Avenue, Changchun, Jilin, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Zhang
authors.forename: Xu
authors.initials: X
authors.lastname: Gong
authors.forename: Shuhai
authors.initials: S
authors.lastname: Han
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003929:Diabetic Neuropathies / Q000235:genetics* / Q000473:pathology*; D015536:Down-Regulation; D005727:Ganglia, Spinal / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D009474:Neurons / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D051900:Smad3 Protein / Q000378:metabolism
chemical_list: C526366:MIRN29 microRNA, rat; D035683:MicroRNAs; D051900:Smad3 Protein