title: Ultrastructural features of endometrial-myometrial interface and its alteration in adenomyosis.
abstract: The endometrial-myometrial interface (EMI) is a specific functional region of uterus. However, our knowledge on EMI ultrastructure both in normal uterus and adenomyosis is far from enough to understand its pathology. In this study, used the samples of EMI and outer myometrium (OM) from the adenomyosis hysterectomy specimens and the subjects from the control uteri, we prospectively compared the ultrastructure of myocytes from EMI and OM, the ultrastructural changes of EMI between the proliferative and secretory phases, and the ultrastructural difference of EMI between adenomyosis and the control group. In both adenomyosis and control group, there were differences in ultrastructure between myocytes from EMI and OM. Specifically, the myocytes from EMI were rich in organelles. In contrast, the myocytes from OM had abundant contractile structural components. In the proliferative phase, the myocytes from EMI in adenomyosis had significantly smaller cell and nucleus diameter than those from the control group, but in the secretory phase, the difference was not significant. In the control group, the various ultrastructural features of myocytes from EMI including the mean diameter of cell and nuclei and the myofilaments/cytoplasm ratio exhibited cyclical changes, but in adenomyosis, the normal cyclical changes were absent. In conclusions, there are significant ultrastructural differences between the myocytes from EMI and OM. The myocytes in women with adenomyosis were significantly different to the control subjects, primarily because the normal cyclical changes were absent.
authors.affiliation: Department of Gynecology Minimally Invasive Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University Qi He Lou Street 17#, Dongcheng District, Beijing, China, Post code: 100006.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Gynecology Minimally Invasive Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University Qi He Lou Street 17#, Dongcheng District, Beijing, China, Post code: 100006.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Reproductive Medicine and Surgery, The Jessop Wing Sheffield Teaching Hospital The Jessop Wing Tree Root Walk, Sheffield, United Kingdom, Post code: S10 2SF.
authors.forename: Tin C
authors.initials: TC
authors.lastname: Li
authors.affiliation: Department of Gynecology Minimally Invasive Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University Qi He Lou Street 17#, Dongcheng District, Beijing, China, Post code: 100006.
authors.forename: Hua
authors.initials: H
authors.lastname: Duan
authors.affiliation: Department of Gynecology Minimally Invasive Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University Qi He Lou Street 17#, Dongcheng District, Beijing, China, Post code: 100006.
authors.forename: Pei
authors.initials: P
authors.lastname: Yu
authors.affiliation: Department of Pathology, Fu Wai Hospital Cardiovascular Institute, Chinese Academy of Medical Sciences Peking Union Medical College Beijing, China, Post code: 100037.
authors.forename: Hong Y
authors.initials: HY
authors.lastname: Wang
mesh_terms: D062788:Adenomyosis / Q000473:pathology* / Q000503:physiopathology; D000328:Adult; D002450:Cell Communication / Q000502:physiology; D004717:Endometrium / Q000473:pathology / Q000503:physiopathology / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D008597:Menstrual Cycle / Q000502:physiology; D008875:Middle Aged; D032342:Muscle Cells / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure; D009215:Myometrium / Q000473:pathology / Q000503:physiopathology / Q000648:ultrastructure*
keywords: Adenomyosis; endometrial-myometrial interface; outer myometrium; ultrastructure