title: Soluble L-Selectin as an Independent Biomarker of Bronchial Asthma.
abstract: BACKGROUND
  We sought to determine the association of plasma level of soluble L-selectin (sL-selectin) and F206L polymorphism of L-selectin with asthma.
  
  
  METHODS
  A total of 90 asthmatic patients and 90 sex- and age-matched healthy controls were enrolled. The plasma level of sL-selectin was measured by enzyme-linked immunosorbent assay (ELISA) method. An amplification refractory mutation system polymerase chain reaction was performed to detect F206L polymorphism of L-selectin.
  
  
  RESULTS
  The mean plasma levels of sL-selectin was significantly higher in the patients with asthma than the controls (2113 ± 466 vs. 1664 ± 322 ng/ml, P = 0.001). Logistic regression analysis after adjustment for age, sex, and body mass index demonstrated that plasma levels of sL-selectin are an independent biomarkers for asthma (odds ratio [OR], 1.86; 95% confidence interval [95% CI], 1.42-2.24). The area under the receiver operating characteristic (ROC) curve for sL-selectin was 0.792, 95% CI (0.732-0.862), P = 0.0001. Individuals with the minor homozygote of F206L polymorphism of L-selectin demonstrated a higher level of sL-selectin than the major homozygous (2319 ± 732 vs. 1917 ± 453 ng/ml, P = 0.02). No association was found between F206L polymorphism of L-selectin with asthma.
  
  
  CONCLUSIONS
  Our study suggests that plasma level of sL-selectin is an independent biomarker for asthma.
authors.affiliation: Division of Pulmonary Sciences and Critical Care Medicine, Hamadan Beheshti Hospital, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Nadi
authors.affiliation: Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hajilooi
authors.affiliation: Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Pajouhan
authors.affiliation: University of Texas Health Science Center at Houston, Houston, Texas.|Texas Heart Institute, Houston, Texas.
authors.forename: Mehran
authors.initials: M
authors.lastname: Haidari
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood* / Q000235:genetics; D015415:Biomarkers / Q000097:blood*; D005260:Female; D005838:Genotype; D006801:Humans; D019041:L-Selectin / Q000097:blood* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics
chemical_list: D015415:Biomarkers; D019041:L-Selectin
keywords: asthma; biomarker; genetic polymorphism; receiver operating characteristic; soluble l-selectin