title: Characterisation of seven newly established head and neck squamous cell carcinoma cell lines.
abstract: Seven squamous cell carcinoma cell lines were disintegrated from biopsies of patients with head and neck cancer. Genotyping tests verified the authenticity and the human origin of all seven lines. The cell lines designated as University of Kiel, Head and Neck (UKHN) -1 to -3 and UKHN-6 to -9 were subjected to flow cytometry and indirect immunofluorescence to assess aberrant DNA content. To confirm the squamous epithelial origin of the cells, the cytokeratin profile was immunocytologically analysed. The cell lines showed individual differences in mitotic frequency. UKHN-1, -6, -7 and -9 grew as monolayers, whereas UKHN-2, -3, and -8 tend to multilayer stratification. Overexpression of LOXL4 and Pim-1 proteins as distinctive features of head and neck carcinomas were shown in all seven cell lines. Inoculating SCID mice with these cell lines resulted in tumour formation, hence corroborating the tumourigenicity of all seven cell lines. The cell lines were also tested for high-risk HPV types using different DNA-based assays and found to be negative.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Christian-Albrechts University Kiel, Kiel, Germany, gorogh@hno.uni-kiel.de.
authors.forename: Tibor
authors.initials: T
authors.lastname: Görögh
authors.forename: Elgar Susanne
authors.initials: ES
authors.lastname: Quabius
authors.forename: Patrick
authors.initials: P
authors.lastname: Meyer
authors.forename: Markus
authors.initials: M
authors.lastname: Hoffmann
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D016513:Mice, SCID; D023421:Models, Animal; D008249:Protein-Lysine 6-Oxidase; D051573:Proto-Oncogene Proteins c-pim-1 / Q000235:genetics; D000077195:Squamous Cell Carcinoma of Head and Neck; D059467:Transcriptome
chemical_list: D000594:Amino Acid Oxidoreductases; C445682:LOXL4 protein, human; D008249:Protein-Lysine 6-Oxidase; C496634:PIM1 protein, human; D051573:Proto-Oncogene Proteins c-pim-1