title: A competitive PCR assay confirms the association of a copy number variation in the VIPR2 gene with schizophrenia in Han Chinese.
abstract: Evidence from genetic studies has revealed that genome-wide rare copy number variations (CNVs) are risk factors for neurodevelopmental disorders and this evidence has given rise to a new understanding of disease etiology, including that of schizophrenia (SCZ). Recent studies have indicated that duplication in the vasoactive intestinal peptide receptor-2 (VIPR2) gene confers the susceptibility to SCZ in Caucasians, but so far this finding has still not been confirmed in Chinese populations. In this study, we investigated the association between CNVs in VIPR2 and SCZ risk in an independent case-control study of Han Chinese using 1035 cases and 1535 controls. The CNVs were genotyped using the multiplex fluorescence competitive PCR method. In contrast with a common genotype (2-copy), a microduplication variant genotype (3-copy) was only carried by SCZ patients (4/1035). This finding indicated that CNVs in VIPR2 may impose a significantly increased risk of SCZ in Han Chinese (P=0.02646, OR=infinity, 95% CI=1.327-infinity). Thus, our results suggest that carriers of microduplication genotypes of VIPR2 are predisposed to SCZ in Han Chinese.
authors.affiliation: Wuxi Mental Health Center, Wuxi 214151, Jiangsu Province, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Wuxi Mental Health Center, Wuxi 214151, Jiangsu Province, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Jin
authors.affiliation: Yangzhou Wutaishan Hospital, Yangzhou, Jiangsu Province, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Sha
authors.affiliation: Wuxi Mental Health Center, Wuxi 214151, Jiangsu Province, China.
authors.forename: Zhenhe
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Wuxi Mental Health Center, Wuxi 214151, Jiangsu Province, China.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Nanjing Qinglongshan Mental Hospital, Nanjing, Jiangsu Province, China.
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Wang
authors.affiliation: Wuxi Mental Health Center, Wuxi 214151, Jiangsu Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Nanjing Qinglongshan Mental Hospital, Nanjing, Jiangsu Province, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Li
authors.affiliation: Yangzhou Wutaishan Hospital, Yangzhou, Jiangsu Province, China.
authors.forename: Xuwei
authors.initials: X
authors.lastname: Feng
authors.affiliation: Wuxi Mental Health Center, Wuxi 214151, Jiangsu Province, China.
authors.forename: Shui
authors.initials: S
authors.lastname: Yu
authors.affiliation: Wuxi Mental Health Center, Wuxi 214151, Jiangsu Province, China. Electronic address: wangjd9178@126.com.
authors.forename: Jidong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000328:Adult; D044466:Asian People / Q000208:ethnology / Q000235:genetics; D056915:DNA Copy Number Variations / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D051239:Receptors, Vasoactive Intestinal Peptide, Type II / Q000235:genetics*; D012559:Schizophrenia / Q000208:ethnology / Q000235:genetics*
chemical_list: D051239:Receptors, Vasoactive Intestinal Peptide, Type II; C495509:VIPR2 protein, human
keywords: Copy number variants; Han Chinese; Schizophrenia; VIPR2