title: Variation in N-acetyltransferase 2 (NAT2), smoking and risk of prostate cancer in the Slovak population.
abstract: N-acetyltransferase 2 (NAT2) is an enzyme involved in the biotransformation of xenobiotics, mainly aromatic and heterocyclic amines and hydrazines, all of which represent an important class of carcinogens found in tobacco smoke. Polymorphism in NAT2 gene is reported to be associated with susceptibility to various types of cancer. This study investigated the relationship between the NAT2 polymorphism and the risk of prostate cancer with reference to the link between cigarette smoking and the xenobiotic-metabolizing enzyme NAT2. Overall, 281 cases and 395 controls from Slovakia were studied using polymerase chain reaction-restriction fragment length polymorphism assay. We found no statistically significant association between NAT2 genotypes and prostate cancer risk (slow acetylation vs. rapid acetylation: OR 1.13; 95 % CI 0.83-1.55). We report here a statistically significant correlation between the NAT2*5C/NAT2*6A slow acetylator genotype and the risk for developing prostate cancer (OR 2.91; 95 % CI 1.43-5.94; p = 0.003) when compared with the rapid phenotype. Smokers with NAT2 rapid phenotype had a five percent (5 %) reduced risk of prostate cancer compared with non-smokers carrying the rapid acetylator genotype. The association was reversed among smokers and non-smokers with NAT2 slow phenotype. On the basis of the foregoing, we conclude that the NAT2 phenotypes whether alone or in association with smoking do not correlate with susceptibility to prostate cancer within the Slovak population.
authors.affiliation: Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University in Bratislava, Malá Hora 4, 03601, Martin, Slovakia.
authors.forename: Marta
authors.initials: M
authors.lastname: Vilčková
authors.forename: Jana
authors.initials: J
authors.lastname: Jurečeková
authors.forename: Dušan
authors.initials: D
authors.lastname: Dobrota
authors.forename: Viera
authors.initials: V
authors.lastname: Habalová
authors.forename: Lucia
authors.initials: L
authors.lastname: Klimčáková
authors.forename: Iveta
authors.initials: I
authors.lastname: Waczulíková
authors.forename: Peter
authors.initials: P
authors.lastname: Slezák
authors.forename: Ján
authors.initials: J
authors.lastname: Kliment
authors.forename: Monika Kmeťová
authors.initials: MK
authors.lastname: Sivoňová
mesh_terms: D000107:Acetylation; D000368:Aged; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease; D005828:Genetics, Population; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D018154:Slovakia; D012907:Smoking / Q000235:genetics*
chemical_list: D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human