title: Expression profiles of myostatin, myogenin, and Myosin heavy chain in skeletal muscles of two rabbit breeds differing in growth rate.
abstract: The purpose of the present study was to compare mRNA levels of myostatin (MSTN), myogenin (MyoG), and fiber type compositions in terms of myosin heavy chain (MyHC) in skeletal muscles of two rabbit breeds with different body sizes and growth rates. Longissimus dorsi and biceps femoris muscles of 16 Californian rabbits (CW) and 16 Germany great line of ZIKA rabbits (GZ) were collected at the ages of 35d and 84d (slaughter age). The results showed that the live weights of GZ rabbits of 35d and 84d old were approximately 36% and 26% greater than those of CW rabbits, respectively. Quantitative real-time PCR analysis revealed that at the age of 84d GZ rabbits contained significantly lower MSTN mRNA level and higher MyoG mRNA level in both longissimus dorsi and biceps femoris muscles than CW rabbits, and mRNA levels of MSTN and MyoG exhibited opposite changes from the age of 35d to 84d, suggesting that GZ rabbits were subjected to less growth inhibition from MSTN at slaughter age, which occurred most possibly in skeletal muscles. Four types of fiber were identified by real-time PCR in rabbit muscles, with MyHC-1 and MyHC-2D, MyHC-2B were the major types in biceps femoris and longissimus dorsi muscles, respectively. At the age of 84d, GZ rabbits contained greater proportion of MyHC-1 and decreased proportion of MyHC-2D and decreased lactate dehydrogenase activity in biceps femoris than CW rabbits, and the results were exactly opposite in longissimus dorsi, suggesting that GZ rabbits show higher oxidative capacity in biceps femoris muscle than CW rabbits. In conclusion, the trends of mRNA levels of MSTN and fiber types in GZ rabbits' skeletal muscles might be consistent with the putative fast growth characteristic of GZ rabbits compared to CW rabbits.
authors.affiliation: a Sichuan Animal Sciences Academy , Chengdu , China.
authors.forename: Liangde
authors.initials: L
authors.lastname: Kuang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Xie
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Lei
authors.forename: Congyan
authors.initials: C
authors.lastname: Li
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Ren
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Guo
authors.forename: Cuixia
authors.initials: C
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.forename: Yucai
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D058751:Livestock; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D018008:Myogenin / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D055435:Myostatin / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D011817:Rabbits / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D013045:Species Specificity
chemical_list: D018008:Myogenin; D055435:Myostatin; D018995:Myosin Heavy Chains
keywords: Gene expression; Myogenin; Myosin heavy chain; Myostatin; Rabbit