title: TILLING for plant breeding.
abstract: TILLING is widely used in plant functional genomics. Mutagenesis and SNP detection is combined to allow for the isolation of mutations in genes of interest. It can also be used as a plant breeding tool, whereby variation in known or candidate genes of interest to breeding programs is generated. Here we describe a simple low-cost TILLING procedure.
authors.affiliation: Plant Breeding Institute, University of Sydney, Private Bag 4011, Narellan, NSW, 2567, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Sharp
authors.forename: Chongmei
authors.initials: C
authors.lastname: Dong
mesh_terms: D001947:Breeding / Q000379:methods*; D004252:DNA Mutational Analysis; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D017343:Genes, Plant; D016296:Mutagenesis; D059645:Mutation Rate; D020641:Polymorphism, Single Nucleotide; D012639:Seeds / Q000235:genetics; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant