title: High-level exogenous trans10, cis12 conjugated linoleic acid plays an anti-lipogenesis role in bovine mammary epithelial cells.
abstract: Primary bovine mammary epithelial cells (BMECs) were treated by 0, 37.5, 75, 112.5, 150 μmol/L trans10, cis12 conjugated linoleic acid (CLA) to evaluate the effects of different level trans10, cis12 CLA on lipogenesis in BMEC. Addition of 75-150 μmol/L trans10, cis12 CLA reduced significantly the triacylglycerol (TAG) content (P<0.05), but did not have inhibiting action on cell proliferation (P>0.05). Treatment with 150 μmol/L trans10, cis12 CLA for 48 h resulted in a 17.1% reduction (P<0.0001) of medium chain fatty acids (MCFA, C14<C<C16), a 26.5% reduction (P<0.0001) of unsaturated fatty acids (UFA) and a corresponding reduction of the mRNA abundance of acetyl coenzyme A (acetylCoA) carboxylase (ACC) (P=0.046), fatty acid synthase (FAS) (P=0.017) and stearoylCoA desaturase1 (SCD1) (P=0.002). Another finding was that trans10, cis12 CLA elevated expression of diacylglycerol acyltransferase2 (DGAT2) (P=0.020) and long chain acylCoA synthetases (ACSL) (P=0.032). In conclusion, higher trans10, cis12 CLA, not low trans10, cis12 CLA, inhibited milk fat synthesis and changed fatty acid composition by regulating the expression of FAS, ACC, SCD1, DGAT2 and ACSL.
authors.affiliation: Institute of Dairy Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, China; Department of Animal Sciences and Technology, Shandong Agricultural University, Taian, China.
authors.forename: Hongfang
authors.initials: H
authors.lastname: Wang
authors.forename: Hongyun
authors.initials: H
authors.lastname: Liu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Liu
authors.forename: Ke
authors.initials: K
authors.lastname: Zhao
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Weiren
authors.initials: W
authors.lastname: Yang
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003066:Coenzyme A Ligases / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D050155:Lipogenesis / Q000187:drug effects*; D008321:Mammary Glands, Animal / Q000166:cytology*; D008892:Milk / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D044243:Linoleic Acids, Conjugated; D012333:RNA, Messenger; D014280:Triglycerides; C496197:trans-10,cis-12-conjugated linoleic acid; D013230:Stearoyl-CoA Desaturase; D051048:Diacylglycerol O-Acyltransferase; D064429:Fatty Acid Synthases; D003066:Coenzyme A Ligases; D000103:Acetyl-CoA Carboxylase
keywords: bovine; cis12 CLA; fatty acid composition; mammary epithelial cells; trans10; triacylglycerol