title: [Cloning and bioinformatics analysis of rhoptry protein 11 of Toxoplasma gondii].
abstract: Total RNA was extracted from tachyzoites of RH strain of Toxoplasma gondii. The open reading frame of ROP11 gene was amplified by using a pair of specific primers designed according to the coding sequence of ROP11 gene (Accession No. DQ077905). The RT-PCR product was digested by restriction enzyme EcoR I and Not I, and then ligated into a pGEX-6P-2 vector. The recombinant plasmid was transferred into E. coli XL-Blue. The positive clones was selected by colony PCR, and confirmed by the double restriction enzyme digestion and sequencing. The RT-PCR product was 1,548 bp. The recombinant plasmid was confirmed by colony PCR and double restriction enzyme digestion. Sequencing results showed that the obtained ROP11 gene was 1 548 bp (Accession No. KC456639). There was a high sequence consistency (99%) between the obtained ROP11 gene sequence and the Toxoplasma ROP11 gene from GenBank. Bioinformatics analysis showed that the ROP11 protein (Mr 57,020) consisted of the signal peptide (amino acids 1-26), 12 conservative domains, a serine/threonine protein kinase catalytic domain (amino acids 170-511), and two potential N-glycosylation sites.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Zhang
authors.forename: Jin-Shun
authors.initials: JS
authors.lastname: Zhang
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Jia
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chun-Miao
authors.initials: CM
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Lu
authors.forename: Jian-Ling
authors.initials: JL
authors.lastname: Zhao
authors.forename: Tian-Jun
authors.initials: TJ
authors.lastname: Jia
mesh_terms: D003001:Cloning, Molecular; D019295:Computational Biology; D015870:Gene Expression; D005822:Genetic Vectors; D015800:Protozoan Proteins / Q000235:genetics*; D014122:Toxoplasma / Q000235:genetics*
chemical_list: D015800:Protozoan Proteins