title: Up-regulation of podoplanin involves in neuronal apoptosis in LPS-induced neuroinflammation.
abstract: Podoplanin (PDPN) is a mucin-type transmembrane sialoglycoprotein expressed in multiple tissues in adult animals, including the brain, lungs, kidney, and lymphoid organs. Studies of this molecule have demonstrated its great importance in tumor metastasis, platelet aggregation, and lymphatic vessel formation. However, information regarding its regulation and possible function in the central nervous system is still limited. In this study, we performed a neuroinflammatory model by lipopolysaccharide (LPS) lateral ventral injection in adult rats and detected increased expression of PDPN in the brain cortex. Immunofluorescence indicated that PDPN was located in the neurons, but not astrocytes. Moreover, there was a concomitant up-regulation of active caspase-3, cyclin D1, and CDK4 in vivo and vitro studies. In addition, the expression of these three proteins in cortical primary neurons was decreased after knocking down PDPN by siRNA. Collectively, all these results suggested that the up-regulation of PDPN might be involved in neuronal apoptosis in neuroinflammation after LPS injection.
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.forename: Jianhong
authors.initials: J
authors.lastname: Shen
authors.forename: Yuchang
authors.initials: Y
authors.lastname: Lin
authors.forename: Jiabing
authors.initials: J
authors.lastname: Shen
authors.forename: Xinming
authors.initials: X
authors.lastname: Wu
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Yan
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Maohong
authors.initials: M
authors.lastname: Cao
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008562:Membrane Glycoproteins / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015533:Transcriptional Activation; D015854:Up-Regulation / Q000187:drug effects; D000098853:Podoplanin
chemical_list: D053148:Caspase 3; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D034741:RNA, Small Interfering; C092025:Pdpn protein, rat; D000098853:Podoplanin