title: The protective effect of apelin against water-immersion and restraint stress-induced gastric damage.
abstract: The aim of the present study was to investigate the gastroprotective effect of apelin on water-immersion and restraint stress (WIRS)-induced gastric lesions. Male Wistar rats were divided into four groups: control, WIRS, F13A + WIRS and F13A. APJ receptor antagonist F13A was administered to rats to determine the influence of apelin on stress-induced gastric injury. WIRS administered for 6 h resulted in the development of gastric mucosal lesions accompanied by a significant increase in plasma corticosterone. WIRS increased the concentration of 4-hydroxynonenol (4-HNE) + malondialdehyde (MDA) and the expression of apelin and hypoxia inducible factor-1α (HIF-1α) in gastric mucosa. In addition, WIRS reduced the mucosal blood flow and gastric prostaglandin E(2) (PGE(2)) concentration. Plasma corticosterone, which was increased due to stress, was significantly decreased in the F13A + WIRS group. Gastric lesions and the 4-HNE + MDA concentration were also higher in the F13A + WIRS compared to the WIRS group. We conclude that apelin has a gastroprotective effect against stress-induced lesions possibly by reducing lipid peroxidation in gastric mucosa.
authors.affiliation: Department of Physiology, Faculty of Medicine, Akdeniz University, 07070, Antalya, Turkey, nimetu@akdeniz.edu.tr.
authors.forename: V Nimet
authors.initials: VN
authors.lastname: Izgüt-Uysal
authors.forename: Burcu
authors.initials: B
authors.lastname: Gemici
authors.forename: Ilknur
authors.initials: I
authors.lastname: Birsen
authors.forename: Nuray
authors.initials: N
authors.lastname: Acar
authors.forename: Ismail
authors.initials: I
authors.lastname: Üstünel
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000073861:Apelin; D003345:Corticosterone / Q000097:blood / Q000378:metabolism; D003681:Dehydration / Q000378:metabolism / Q000503:physiopathology*; D015232:Dinoprostone / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism* / Q000503:physiopathology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000502:physiology; D012149:Restraint, Physical / Q000009:adverse effects* / Q000502:physiology; D013270:Stomach / Q000503:physiopathology; D013272:Stomach Diseases / Q000378:metabolism* / Q000503:physiopathology; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D000447:Aldehydes; D000073861:Apelin; C498579:Apln protein, rat; C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D036341:Intercellular Signaling Peptides and Proteins; C532492:apelin-13, Ala(13)-; D008315:Malondialdehyde; C027576:4-hydroxy-2-nonenal; D015232:Dinoprostone; D003345:Corticosterone