title: Rapid replacement of human respiratory syncytial virus A with the ON1 genotype having 72 nucleotide duplication in G gene.
abstract: Human respiratory syncytial virus (HRSV) is the main cause of severe respiratory illness in young children and elderly people. We investigated the genetic characteristics of the circulating HRSV subgroup A (HRSV-A) to determine the distribution of genotype ON1, which has a 72-nucleotide duplication in attachment G gene. We obtained 456 HRSV-A positive samples between October 2008 and February 2013, which were subjected to sequence analysis. The first ON1 genotype was discovered in August 2011 and 273 samples were identified as ON1 up to February 2013. The prevalence of the ON1 genotype increased rapidly from 17.4% in 2011-2012 to 94.6% in 2012-2013. The mean evolutionary rate of G protein was calculated as 3.275 × 10(-3) nucleotide substitution/site/year and several positively selected sites for amino acid substitutions were located in the predicted epitope region. This basic and important information may facilitate a better understanding of HRSV epidemiology and evolution.
authors.affiliation: Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon-gun, Chungbuk-do 363-951, Republic of Korea.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Systems Biology Team, Center for Immunity and Pathology, Korea National Institute of Health, Cheongwon-gun, Chungbuk-do 363-951, Republic of Korea.
authors.forename: Dae-Won
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon-gun, Chungbuk-do 363-951, Republic of Korea.
authors.forename: Wan-Ji
authors.initials: WJ
authors.lastname: Lee
authors.affiliation: Systems Biology Team, Center for Immunity and Pathology, Korea National Institute of Health, Cheongwon-gun, Chungbuk-do 363-951, Republic of Korea.
authors.forename: Mi-Ran
authors.initials: MR
authors.lastname: Yun
authors.affiliation: Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon-gun, Chungbuk-do 363-951, Republic of Korea.
authors.forename: Ho Yeon
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon-gun, Chungbuk-do 363-951, Republic of Korea.
authors.forename: Han Saem
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon-gun, Chungbuk-do 363-951, Republic of Korea.
authors.forename: Hee-Dong
authors.initials: HD
authors.lastname: Jung
authors.affiliation: Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon-gun, Chungbuk-do 363-951, Republic of Korea. Electronic address: tigerkis@gmail.com.
authors.forename: Kisoon
authors.initials: K
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D002648:Child; D002675:Child, Preschool; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D019143:Evolution, Molecular; D005260:Female; D005838:Genotype; D049674:History, 21st Century; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D015995:Prevalence; D056910:Republic of Korea; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology / Q000266:history / Q000276:immunology / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D012621:Seasons; D012641:Selection, Genetic; D016415:Sequence Alignment; D014760:Viral Fusion Proteins / Q000235:genetics*
chemical_list: D000939:Epitopes; C502275:G glycoprotein, Respiratory syncytial virus; D014760:Viral Fusion Proteins
keywords: Attachment G gene; Diversity; Duplication; Evolution; Human respiratory syncytial virus (HRSV)