title: Antioxidant biomarker survey ensuing long-term selenium withdrawal in Acipenser baeri fed Se-cysteine diets.
abstract: Two selenium withdrawal periods, 30 and 90 days, were considered for sturgeon fed 90 days three Se-cysteine diets (1.25, 5, 20 mgkg(-1)). Subsequently Acipenser baeri was fed the previous control diet (0.32 mgSekg(-1)) for 90 days. Levels of superoxide dismutase, catalase, glutathione peroxidases, glutathione reductase, glyoxalase-II and malondialdehyde were determined in liver and kidney. Chemical analyses were carried out for the same tissues and for muscle. A reduction of Se levels in all tissues was recorded and the metalloid concentration decreased more quickly in liver than in kidney and muscle. At the end of the withdrawal Se concentration in muscle remained high in specimens previously fed 20 mgSekg(-1) diet, and disturbance of key antioxidant enzymes was recorded in liver and kidney. Moreover, alterations in glutathione peroxidases, and glyoxalase-II activities persisted even after 90 withdrawal days and were indicative of oxidative stress induced by Se-cysteine concentrations.
authors.affiliation: Department of Cellular and Environmental Biology, University of Perugia, 06123 Perugia, Italy. Electronic address: antonia.elia@unipg.it.
authors.forename: Antonia Concetta
authors.initials: AC
authors.lastname: Elia
authors.affiliation: C.Re.A.A. National Reference Centre for the Surveillance and Monitoring of Animal Feed, State Veterinary Institute, 10154 Turin, Italy.
authors.forename: Maria Cesarina
authors.initials: MC
authors.lastname: Abete
authors.affiliation: Department of Cellular and Environmental Biology, University of Perugia, 06123 Perugia, Italy.
authors.forename: Nicole
authors.initials: N
authors.lastname: Pacini
authors.affiliation: Department of Cellular and Environmental Biology, University of Perugia, 06123 Perugia, Italy.
authors.forename: Ambrosius Josef Martin
authors.initials: AJ
authors.lastname: Dörr
authors.affiliation: Fish Disease Laboratory, State Veterinary Institute, 10154 Turin, Italy.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Scanzio
authors.affiliation: Fish Disease Laboratory, State Veterinary Institute, 10154 Turin, Italy.
authors.forename: Marino
authors.initials: M
authors.lastname: Prearo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000493:pharmacokinetics*; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004032:Diet; D005399:Fishes / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D018384:Oxidative Stress; D012643:Selenium / Q000378:metabolism*; D017279:Selenocysteine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D013482:Superoxide Dismutase / Q000378:metabolism; D013869:Thiolester Hydrolases / Q000378:metabolism; D000099301:Hydroxyacylglutathione Hydrolase
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D002374:Catalase; D005979:Glutathione Peroxidase; D008315:Malondialdehyde; D012643:Selenium; D017279:Selenocysteine; D013482:Superoxide Dismutase; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase
keywords: Antioxidant enzymes; Kidney; Liver; Muscle; Se-cysteine withdrawal; Sturgeon