title: A novel potentiometric sensor based on a poly(anilineboronic acid)/graphene modified electrode for probing sialic acid through boronic acid-diol recognition.
abstract: A novel potentiometric approach was described for probing sialic acid (SA) using a poly(anilineboronic acid)/graphene modified glassy carbon (GC) electrode. The proposed electrode was prepared by electrodeposition of reduced graphene oxide (ERGO) at a GC electrode and then coated with a poly(anilineboronic acid) (PABA) film by electropolymerization of its monomer. Principle of SA detection at the PABA/ERGO/GC electrode was ascribed to a reversible and covalent boronic acid-diol binding which was sensitive to the electrochemical potential of the prepared sensor. The graphene layer introduced on the electrode surface was shown to dramatically improve the sensitivity of the sensor response. Under optimum conditions, the proposed sensor exhibited low detection limit of 0.8 μM with a wide linear range of 2 μM-1.38 mM, high stability, good regeneration, and remarkable selectivity. For the analysis of SA in human blood serum, the high accuracy and good recoveries revealed the great potential in the practical applications.
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal Universtiy, Shangqiu 476000, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal Universtiy, Shangqiu 476000, China. Electronic address: liult05@iccas.ac.cn.
authors.forename: Lantao
authors.initials: L
authors.lastname: Liu
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal Universtiy, Shangqiu 476000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal Universtiy, Shangqiu 476000, China; College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China. Electronic address: xumaotian@sqnc.edu.cn.
authors.forename: Maotian
authors.initials: M
authors.lastname: Xu
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D001897:Boronic Acids / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D019158:N-Acetylneuraminic Acid / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000814:Aniline Compounds; D001897:Boronic Acids; D006108:Graphite; D019158:N-Acetylneuraminic Acid
keywords: Electrodeposition; Graphene; Poly(aniline boronic acid); Potentiometric sensor; Sialic acid