title: Effect of commercially available green and black tea beverages on drug-metabolizing enzymes and oxidative stress in Wistar rats.
abstract: The effect of commercially available green tea (GT) and black tea (BT) drinks on drug metabolizing enzymes (DME) and oxidative stress in rats was investigated. Male Wistar rats were fed a laboratory chow diet and GT or BT drink for 5 weeks. Control rats received de-ionized water instead of the tea drinks. Rats received the GT and BT drinks treatment for 5 weeks showed a significant increase in hepatic microsomal cytochrome P450 (CYP) 1A1 and CYP1A2, and a significant decrease in CYP2C, CYP2E1 and CYP3A enzyme activities. Results of immunoblot analyses of enzyme protein contents showed the same trend with enzyme activity. Significant increase in UDP-glucuronosyltransferase activity and reduced glutathione content in liver and lungs were observed in rats treated with both tea drinks. A lower lipid peroxide level in lungs was observed in rats treated with GT drink. Electrophoretic mobility shift assay revealed that both tea drinks decreased pregnane X receptor binding to DNA and increased nuclear factor-erythroid 2 p45-related factor 2 binding to DNA. These results suggest that feeding of both tea drinks to rats modulated DME activities and reduced oxidative stress in liver and lungs. GT drink is more effective on reducing oxidative stress than BT drink.
authors.affiliation: Department of Nutrition, China Medical University, 91, Hsueh-shih Road, Taichung 404, Taiwan. Electronic address: htyao@mail.cmu.edu.tw.
authors.forename: Hsien-Tsung
authors.initials: HT
authors.lastname: Yao
authors.affiliation: Department of Nutrition, China Medical University, 91, Hsueh-shih Road, Taichung 404, Taiwan.
authors.forename: Ya-Ru
authors.initials: YR
authors.lastname: Hsu
authors.affiliation: Department of Nutrition, China Medical University, 91, Hsueh-shih Road, Taichung 404, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, 500, Lioufeng Road, Wufeng, Taichung 413, Taiwan.
authors.forename: Chong-Kuei
authors.initials: CK
authors.lastname: Lii
authors.affiliation: Department of Nutrition, China Medical University, 91, Hsueh-shih Road, Taichung 404, Taiwan.
authors.forename: Ai-Hsuan
authors.initials: AH
authors.lastname: Lin
authors.affiliation: Department of Nutrition, China Medical University, 91, Hsueh-shih Road, Taichung 404, Taiwan.
authors.forename: Keng-Hao
authors.initials: KH
authors.lastname: Chang
authors.affiliation: Department of Nutrition, China Medical University, 91, Hsueh-shih Road, Taichung 404, Taiwan. Electronic address: lulu0319@mail.cmu.edu.tw.
authors.forename: Hui-Ting
authors.initials: HT
authors.lastname: Yang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002110:Caffeine / Q000097:blood; D028241:Camellia sinensis / Q000737:chemistry*; D002784:Cholesterol / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D019388:Cytochrome P-450 CYP1A2; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics / Q000378:metabolism; D051544:Cytochrome P-450 CYP3A / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D003580:Cytochromes / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D050984:NF-E2 Transcription Factor, p45 Subunit / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D000077297:Pregnane X Receptor; D051381:Rats; D017208:Rats, Wistar; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D013662:Tea / Q000737:chemistry*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003580:Cytochromes; D050984:NF-E2 Transcription Factor, p45 Subunit; D000077297:Pregnane X Receptor; D011987:Receptors, Steroid; D013662:Tea; D014280:Triglycerides; C400322:cytochrome P-450 CYP2C subfamily; D002110:Caffeine; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D019392:Cytochrome P-450 CYP2E1; C017170:Cyp1a2 protein, rat; D019363:Cytochrome P-450 CYP1A1; D019388:Cytochrome P-450 CYP1A2; D051544:Cytochrome P-450 CYP3A; D005978:Glutathione
keywords: Black tea; Drug-metabolizing enzymes; Green tea; Oxidative stress; Rats