title: Successful conversion of the Bacillus subtilis BirA Group II biotin protein ligase into a Group I ligase.
abstract: Group II biotin protein ligases (BPLs) are characterized by the presence of an N-terminal DNA binding domain that allows transcriptional regulation of biotin biosynthetic and transport genes whereas Group I BPLs lack this N-terminal domain. The Bacillus subtilis BPL, BirA, is classified as a Group II BPL based on sequence predictions of an N-terminal helix-turn-helix motif and mutational alteration of its regulatory properties. We report evidence that B. subtilis BirA is a Group II BPL that regulates transcription at three genomic sites: bioWAFDBI, yuiG and yhfUTS. Moreover, unlike the paradigm Group II BPL, E. coli BirA, the N-terminal DNA binding domain can be deleted from Bacillus subtilis BirA without adverse effects on its ligase function. This is the first example of successful conversion of a Group II BPL to a Group I BPL with retention of full ligase activity.
authors.affiliation: Department of Microbiology, University of Illinois, Urbana, Illinois, United States of America.
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Henke
authors.affiliation: Department of Microbiology, University of Illinois, Urbana, Illinois, United States of America; Department of Biochemistry, University of Illinois, Urbana, Illinois, United States of America.
authors.forename: John E
authors.initials: JE
authors.lastname: Cronan
mesh_terms: D000249:Adenosine Monophosphate / Q000031:analogs & derivatives / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology*; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism; D019731:Carbon-Nitrogen Ligases / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D014158:Transcription, Genetic
chemical_list: C082099:5'-AMP-biotin; D000249:Adenosine Monophosphate; D001710:Biotin; D000255:Adenosine Triphosphate; D019731:Carbon-Nitrogen Ligases