title: Scribed transparency microplates mounted on a modified standard microplate.
abstract: The immense cost effectiveness of using transparencies as analyte handling implements in microplate instrumentation offers the possibility of application even in resource-limited laboratories. In this work, a standard microplate was adapted to serve as the permanent base for disposable scribed transparencies. The approach is shown to ameliorate evaporation, which can affect assay accuracy when analytes need to be incubated for some time. It also offers assurance against fluorescence measurement errors due to the cross-talk of samples from adjacent wells.
authors.affiliation: Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Brandon Huey-Ping
authors.initials: BH
authors.lastname: Cheong
authors.affiliation: Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Wei Seong
authors.initials: WS
authors.lastname: Chua
authors.affiliation: Cardiovascular Research Institute, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Centre for Translational Medicine, Singapore 117599, Singapore.
authors.forename: Oi Wah
authors.initials: OW
authors.lastname: Liew
authors.affiliation: Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia. Electronic address: engngtw@gmail.com.
authors.forename: Tuck Wah
authors.initials: TW
authors.lastname: Ng
mesh_terms: D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000592:standards; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000592:standards; D013050:Spectrometry, Fluorescence / Q000592:standards
chemical_list: D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
keywords: Cross-talk; Evaporation; Green fluorescent protein; Microplate; Transparency