title: Identification of a Geranylgeranyl reductase gene for chlorophyll synthesis in rice.
abstract: Geranylgeranyl reductase (CHL P) catalyzes the reduction of geranylgeranyl diphosphate to phytyl diphosphate, and provides phytol for both Chlorophyll (Chl) and tocopherol synthesis. In this study, we isolated a yellow-green leaf mutant, 502ys, in rice (Oryza sativa). The mutant exhibited reduced level of Chls, arrested development of chloroplasts, and retarded growth rate. The phenotype of the 502ys mutant was controlled by by a recessive mutation in a nuclear gene on the long arm of rice chromosome 2. Map-based cloning of the mutant resulted in the identification of an OsChl P gene (LOC_Os02g51080). In the 502ys mutant, a single base pair mutation was detected at residue 1279 in DNA sequence of the gene, resulting in an amino acid change (Gly-206 to Ser) in the encoded protein. HPLC analysis of Chls indicated that the majority of Chl molecules are conjugated with an unsaturated geranylgeraniol side chain, in addition to small amount of normal Chls in the mutant. Furthermore, the mutant phenotype was complemented by transformation with the wild-type gene. Therefore, this study has confirmed the 502ys mutant resulted from a single base pair mutation in OsChl P gene.
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan, China.
authors.forename: Pingyu
authors.initials: P
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan, China.
authors.forename: Changhui
authors.initials: C
authors.lastname: Sun
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan, China.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Gao
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan, China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Deng
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan, China.
authors.forename: Pingrong
authors.initials: P
authors.lastname: Wang
keywords: Chl P gene; Chlorophyll; Geranylgeranyl reductase; Rice