title: Peptide functionalized polyhydroxyalkanoate nanofibrous scaffolds enhance Schwann cells activity.
abstract: Interactions between Schwann cells (SCs) and scaffolds are important for tissue development during nerve regeneration, because SCs physiologically assist in directing the growth of regenerating axons. In this study, we prepared electrospun scaffolds combining poly (3-hydroxybutyrate) (PHB) and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) functionalized with either collagen I, H-Gly-Arg-Gly-Asp-Ser-OH (GRGDS), H-Tyr-Ile-Gly-Ser-Arg-NH2 (YIGSR), or H-Arg-Asn-Ile-Ala-Glu-Ile-Ile-Lys-Asp-Ile-OH (p20) neuromimetic peptides to mimic naturally occurring ECM motifs for nerve regeneration. Cells cultured on fibrous mats presenting these biomolecules showed a significant increase in metabolic activity and proliferation while exhibiting unidirectional orientation along the orientation of the fibers. Real-time PCR showed cells cultured on peptide-modified scaffolds had a significantly higher neurotrophin expression compared to those on untreated nanofibers. Our study suggests that biofunctionalized aligned PHB/PHBV nanofibrous scaffolds may elicit essential cues for SCs activity and could serve as a potential scaffold for nerve regeneration. From the clinical editor: Nanotechnology-based functionalized scaffolds represent one of the most promising approaches in peripheral nerve recovery, as well as spinal cord recovery. In this study, bio-functionalized and aligned PHB/PHBV nanofibrous scaffolds were found to elicit essential cues for Schwann cell activity, therefore could serve as a potential scaffold for nerve regeneration.
authors.affiliation: Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran; Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran; Department of Tissue Regeneration, University of Twente, Enschede, The Netherlands. Electronic address: elahe_mas@tx.iut.ac.ir.
authors.forename: Elahe
authors.initials: E
authors.lastname: Masaeli
authors.affiliation: Department of Tissue Regeneration, University of Twente, Enschede, The Netherlands. Electronic address: p.a.wieringa@utwente.nl.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Wieringa
authors.affiliation: Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran. Electronic address: morshed@cc.iut.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Morshed
authors.affiliation: Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Electronic address: mh_nasr@med.mui.ac.ir.
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: Nasr-Esfahani
authors.affiliation: Department of Electrical and Computer Engineering, Isfahan University of Technology, Isfahan, Iran. Electronic address: sadri@cc.iut.ac.ir.
authors.forename: Saeid
authors.initials: S
authors.lastname: Sadri
authors.affiliation: Department of Tissue Regeneration, University of Twente, Enschede, The Netherlands. Electronic address: c.a.vanblitterwsijk@utwente.nl.
authors.forename: Clemens A
authors.initials: CA
authors.lastname: van Blitterswijk
authors.affiliation: Department of Tissue Regeneration, University of Twente, Enschede, The Netherlands. Electronic address: l.moroni@utwente.nl.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Moroni
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D010455:Peptides / Q000737:chemistry*; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D000091402:Prohibitins; D012583:Schwann Cells / Q000166:cytology*; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds
chemical_list: C000717328:PHB protein, human; D010455:Peptides; D054813:Polyhydroxyalkanoates; D000091402:Prohibitins
keywords: Electrospinning; Peptide; Scaffold; Schwann cells