title: Amyloid fibril formation by β-lactoglobulin is inhibited by gold nanoparticles.
abstract: The endogenous deposition of protein fibrillar aggregates in the tissues is the cause of several neurodisorders. In the present study the native β-lactoglobulin (β-lg) has been driven towards amyloid fibrillar aggregates when it was exposed to heat at 75°C for 1h at pH 7.5. The citrate stabilized gold nanoparticle (AuNPs) of 20nm diameter is shown to inhibit the thermal aggregation of β-lg. The stability of the β-lg against heat stress was assessed by studying its aggregation at 75°C, either in presence or in absence of AuNPs. AuNPs stabilizes the monomeric and dimeric forms of the β-lg inhibiting the nucleation and elongation for the formation of higher aggregates. Incubation of β-lg with AuNPs at 75°C results in the formation of smaller ragged aggregates. Results show that AuNPs possess the capability of inhibiting the formation of amyloid fibrillar aggregates of β-lg in a concentration-dependent manner and may thus facilitate the refolding into native like structure. AuNPs thus serve as nano-chaperon to inhibit the protein aggregation. Thus chaperon like activity of AuNP may be exploited in the design of rational therapeutics for the neurodegenerative diseases.
authors.affiliation: Organic Chemistry Section, Department of Chemistry, Jadavpur University, Kolkata 700032, India.
authors.forename: Subrata
authors.initials: S
authors.lastname: Sardar
authors.affiliation: Organic Chemistry Section, Department of Chemistry, Jadavpur University, Kolkata 700032, India.
authors.forename: Sampa
authors.initials: S
authors.lastname: Pal
authors.affiliation: Organic Chemistry Section, Department of Chemistry, Jadavpur University, Kolkata 700032, India.
authors.forename: Sanhita
authors.initials: S
authors.lastname: Maity
authors.affiliation: Organic Chemistry Section, Department of Chemistry, Jadavpur University, Kolkata 700032, India.
authors.forename: Jishnu
authors.initials: J
authors.lastname: Chakraborty
authors.affiliation: Organic Chemistry Section, Department of Chemistry, Jadavpur University, Kolkata 700032, India. Electronic address: uhalder2002@yahoo.com.
authors.forename: Umesh Chandra
authors.initials: UC
authors.lastname: Halder
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002951:Citrates / Q000737:chemistry; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions; D007782:Lactoglobulins / Q000737:chemistry*; D053768:Metal Nanoparticles; D055503:Protein Multimerization / Q000187:drug effects*; D017433:Protein Structure, Secondary / Q000187:drug effects
chemical_list: D000682:Amyloid; D002951:Citrates; D007782:Lactoglobulins; D006046:Gold
keywords: Amyloid fibril; Gold nanoparticles (AuNP); Inhibition; β-Lactoglobulin (β-lg)