title: MiR-101, downregulated in retinoblastoma, functions as a tumor suppressor in human retinoblastoma cells by targeting EZH2.
abstract: Accumulating evidence indicates that microRNAs are involved in multiple processes in cancer development and progression, and several miRNAs have emerged as candidate components of oncogene or tumor-suppressor networks in retinoblastoma. miR-101 has been identified as a tumor suppressor in several types of human cancer. However, the specific function of miR-101 in retinoblastoma remains unclear. In the present study, we found that the expression of miR-101 in retinoblastoma tissues was much lower than that in the normal controls. In addition, downregulation of miR-101 more frequently occurred in retinoblastoma specimens with adverse clinicopathological and histopathological features. In addition, miR-101 inhibited cell viability and progression in retinoblastoma cells by promoting cell apoptosis and arresting the cell cycle. Finally, we found that miR-101 directly inhibited EZH2 expression by targeting its 3'-UTR, and EZH2 was upregulated and inversely correlated with miR-101 expression in the retinoblastoma tissues. Thus, for the first time, we provide convincing evidence that downregulation of miR-101 is associated with tumor aggressiveness in retinoblastoma and inhibits cell growth and proliferation of retinoblastoma cells by targeting EZH2. In conclusion, all the evidence supports the tumor-suppressor role of miR-101 in human retinoblastoma.
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710062, P.R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Lei
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710062, P.R. China.
authors.forename: Fengmei
authors.initials: F
authors.lastname: Shen
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710062, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Ophthalmology, No.1 Hospital of Xi'an, Xi'an, Shaanxi 710062, P.R. China.
authors.forename: Weishan
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, No.1 Hospital of Xi'an, Xi'an, Shaanxi 710062, P.R. China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710062, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D002648:Child; D002675:Child, Preschool; D000071221:Enhancer of Zeste Homolog 2 Protein; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D007223:Infant; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D063151:Polycomb Repressive Complex 2 / Q000235:genetics* / Q000378:metabolism; D019572:Retinal Neoplasms / Q000235:genetics* / Q000473:pathology*; D012175:Retinoblastoma / Q000235:genetics* / Q000473:pathology*
chemical_list: C534072:MIRN101 microRNA, human; D035683:MicroRNAs; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2