title: A rapid qPCR method for genetic sex identification of Salmo salar and Salmo trutta including simultaneous elucidation of interspecies hybrid paternity by high-resolution melt analysis.
abstract: This study presents an improved duplex quantitative polymerase chain reaction (qPCR) method using the master sex-determining gene sdY as a marker for simultaneous genetic sex identification of salmonids of the Salmo genus and paternity elucidation for Salmo salar × Salmo trutta hybrids. This method will provide a new, simple and economical molecular tool for ecological studies of these species as well as for aquaculture purposes.
authors.affiliation: Norwegian Institute of Water Research, N-0349 Oslo, Norway.
authors.forename: M B
authors.initials: MB
authors.lastname: Anglès d'Auriac
authors.forename: H A
authors.initials: HA
authors.lastname: Urke
authors.forename: T
authors.initials: T
authors.lastname: Kristensen
mesh_terms: D000818:Animals; D001483:Base Sequence; D002678:Chimera / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012487:Salmonidae / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012732:Sex Determination Analysis / Q000379:methods*
chemical_list: D005819:Genetic Markers
keywords: aquaculture; fish ecology; molecular diagnostic; molecular sexing; sdY gene; species substitution