title: Low level laser therapy reduces acute lung inflammation in a model of pulmonary and extrapulmonary LPS-induced ARDS.
abstract: The present study aimed to investigate the effects low level laser therapy (LLLT) in a LPS-induced pulmonary and extrapulmonary acute respiratory distress syndrome (ARDS) in BALB/c mice. Laser (830nm laser, 9J/cm(2), 35mW, 80s per point, 3 points per application) was applied in direct contact with skin, 1h after LPS administration. Mice were distributed in control (n=6; PBS), ARDS IT (n=7; LPS orotracheally 10μg/mouse), ARDS IP (n=7; LPS intra-peritoneally 100μg/mouse), ARDS IT+Laser (n=9; LPS intra-tracheally 10μg/mouse), ARDS IP+Laser (n=9; LPS intra-peritoneally 100μg/mouse). Twenty-four hours after last LPS administration, mice were studied for pulmonary inflammation by total and differential cell count in bronchoalveolar lavage (BAL), cytokines (IL-1beta, IL-6, KC and TNF-alpha) levels in BAL fluid and also by quantitative analysis of neutrophils number in the lung parenchyma. LLLT significantly reduced pulmonary and extrapulmonary inflammation in LPS-induced ARDS, as demonstrated by reduced number of total cells (p<0.001) and neutrophils (p<0.001) in BAL, reduced levels of IL-1beta, IL-6, KC and TNF-alpha in BAL fluid and in serum (p<0.001), as well as the number of neutrophils in lung parenchyma (p<0.001). LLLT is effective to reduce pulmonary inflammation in both pulmonary and extrapulmonary model of LPS-induced ARDS.
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil.
authors.forename: Manoel Carneiro
authors.initials: MC
authors.lastname: Oliveira
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil.
authors.forename: Flávia Regina
authors.initials: FR
authors.lastname: Greiffo
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil.
authors.forename: Nicole Cristine
authors.initials: NC
authors.lastname: Rigonato-Oliveira
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil.
authors.forename: Ricardo Wesley Alberca
authors.initials: RW
authors.lastname: Custódio
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil.
authors.forename: Vanessa Roza
authors.initials: VR
authors.lastname: Silva
authors.affiliation: University of Sao Paulo, School of Medicine, Department of Pathology (LIM 59), Av. Doutor Arnaldo 455, São Paulo, SP CEP 01246-000, Brazil.
authors.forename: Nilsa Regina
authors.initials: NR
authors.lastname: Damaceno-Rodrigues
authors.affiliation: University of Sao Paulo, School of Medicine, Department of Clinical Medicine (LIM 20), Av. Doutor Arnaldo 455, São Paulo, SP CEP 01246-000, Brazil.
authors.forename: Francine Maria
authors.initials: FM
authors.lastname: Almeida
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil.
authors.forename: Regiane
authors.initials: R
authors.lastname: Albertini
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil.
authors.forename: Rodrigo Álvaro B
authors.initials: RÁ
authors.lastname: Lopes-Martins
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil.
authors.forename: Luis Vicente Franco
authors.initials: LV
authors.lastname: de Oliveira
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil.
authors.forename: Paulo de Tarso Camillo
authors.initials: Pde T
authors.lastname: de Carvalho
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Ligeiro de Oliveira
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil.
authors.forename: Ernesto César P
authors.initials: EC
authors.lastname: Leal
authors.affiliation: Nove de Julho University, Rua Vergueiro 239/245, São Paulo, SP CEP 01504-000, Brazil. Electronic address: rodrelena@yahoo.com.br.
authors.forename: Rodolfo P
authors.initials: RP
authors.lastname: Vieira
mesh_terms: D000208:Acute Disease; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D007249:Inflammation / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D028022:Low-Level Light Therapy; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000166:cytology; D012128:Respiratory Distress Syndrome / Q000209:etiology / Q000473:pathology / Q000532:radiotherapy*
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides
keywords: ARDS; Bronchoalveolar lavage; Cytokines; LLLT; LPS; Lung inflammation