title: [Tissue localization and expression difference of endogenous beta-glucosidase in digestive system of Musca domestica third instar larvae].
abstract: OBJECTIVE
  To study the tissue localization and expression difference of endogenous beta-glucosidase in digestive system of Musca domestica third instar larvae.
  
  
  METHODS
  The digestive system of the 3rd instar larvae of Musca domestic was taken for the below tests. Tissue localization of endogenous beta-glucosidase mRNA was identified by in situ hybridization. Cellulase was localized by immunohistochemistry. The enzymatic activity of beta-glucosidase was measured by 3, 5-dinitrosalicylic acid(DNS) assay. The relative mRNA expression levels of M. domestica beta-glucosidase gene in these organs were determined by RT-PCR.
  
  
  RESULTS
  Beta-glucosidase mRNA, with in situ hybridization, was shown in the epithelial cells of midgut, salivary glands and foregut of the larvae. The immunohistochemical analysis on larvae tissues revealed that cellulase was produced and secreted by the epithelial cells of the midgut, salivary glands and foregut. beta-glucosidase activity in salivary glands, foregut, midgut, and hindgut was (0.80 +/- 0.06), (0.38 +/- 0.02), (1.20 +/- 0.05) and (0.26 +/- 0.02) IU/mg, respectively. There was significant difference in beta-glucosidase activity among these digestive organs (P < 0.05). The activity level of beta-glucosidase was highest in midgut [(45.45 +/- 1.27)%], and lowest in hindgut [(9.85 +/- 0.88)%]. However, beta-glucosidase gene were only expressed in the salivary gland, foregut and midgut. Significant differences in gene expression level of beta-glucosidase was found among these organs (P < 0.05). The relative expression quantity of beta-glucosidase gene in midgut and salivary glands were 5 and 3 times higher than that in foregut.
  
  
  CONCLUSIONS
  The endogenous beta-glucosidase gene is expressed in the foregut, midgut and salivary glands. The midgut and salivary glands of Musca domestica 3rd instar larvae are the primary organs of this enzyme secretion.
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Wu
authors.forename: Guo
authors.initials: G
authors.lastname: Guo
authors.forename: Ping
authors.initials: P
authors.lastname: Fu
mesh_terms: D000818:Animals; D004064:Digestive System / Q000201:enzymology*; D006793:Houseflies / Q000201:enzymology*; D007814:Larva / Q000201:enzymology; D012469:Salivary Glands / Q000201:enzymology; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D001617:beta-Glucosidase