title: Histological analysis of the periodontal ligament and alveolar bone during dental movement in diabetic rats subjected to low-level laser therapy.
abstract: OBJECTIVE
  The purpose of this research was to evaluate the histological changes of the periodontal ligament and alveolar bone during dental movement in diabetic rats subjected to low level laser therapy (LLLT).
  
  
  METHODS
  The movement of the upper molar was performed in 60 male Wistar rats divided into four groups (n=15): CTR (control), DBT (diabetic), CTR/LT (irradiated control) and DBT/LT (irradiated diabetic). Diabetes was induced with alloxan (150 mg/kg, i.p.). LLLT was applied with GaAlAs laser at 780 nm (35 J/cm(2)). After 7, 13 and 19 days, the periodontal ligament and alveolar bone were histologically analyzed.
  
  
  RESULTS
  The mean of osteoblasts (p<0.01) and blood vessels (p<0.05) were significantly decreased in DBT compared with CTR at 7 days, whereas the mean of osteoclasts was lower at 7 (p<0.001) and 13 days (p<0.05). In DBT/LT, only the mean of osteoclasts was lower than in CTR (p<0.05) at 7 days, but no difference was observed at 13 and 19 days (p>0.05). The collagenization of the periodontal ligament was impaired in DBT, whereas DBT/LLT showed density/disposition of the collagen fibers similar to those observed in CTR.
  
  
  CONCLUSIONS
  LLLT improved the periodontal ligament and alveolar bone remodeling activity in diabetic rats during dental movement.
authors.affiliation: School of Dentistry, University Tiradentes (UNIT), Rua Terêncio Sampaio, 309, Grageru, Aracaju 49025700, Sergipe, Brazil. Electronic address: orthomaia2003@yahoo.com.br.
authors.forename: Luiz Guilherme Martins
authors.initials: LG
authors.lastname: Maia
authors.affiliation: Laboratory of Morphology and Structural Biology, Science and Technology Institute (ITP), Avenida Murilo Dantas, 300, Prédio do ITP, Farolândia, Aracaju 49032-490, Sergipe, Brazil. Electronic address: angela.dilela@yahoo.com.br.
authors.forename: Angela Valéria Farias
authors.initials: AV
authors.lastname: Alves
authors.affiliation: Laboratory of Morphology and Structural Biology, Science and Technology Institute (ITP), Avenida Murilo Dantas, 300, Prédio do ITP, Farolândia, Aracaju 49032-490, Sergipe, Brazil. Electronic address: talibiomed12@gmail.com.
authors.forename: Talita Santos
authors.initials: TS
authors.lastname: Bastos
authors.affiliation: Laboratory of Morphology and Structural Biology, Science and Technology Institute (ITP), Avenida Murilo Dantas, 300, Prédio do ITP, Farolândia, Aracaju 49032-490, Sergipe, Brazil. Electronic address: lucasmoromizato@hotmail.com.
authors.forename: Lucas Sandes
authors.initials: LS
authors.lastname: Moromizato
authors.affiliation: Laboratory of Morphology and Structural Biology, Science and Technology Institute (ITP), Avenida Murilo Dantas, 300, Prédio do ITP, Farolândia, Aracaju 49032-490, Sergipe, Brazil. Electronic address: isabel_limaverde@yahoo.com.br.
authors.forename: Isabel Bezerra
authors.initials: IB
authors.lastname: Lima-Verde
authors.affiliation: Department of Dentistry, Federal University of Sergipe (UFS), Avenida Cláudio Batista, 54, Sanatório, Aracaju 49000-000, Sergipe, Brazil. Electronic address: endoribeiro@yahoo.com.br.
authors.forename: Maria Amália Gonzaga
authors.initials: MA
authors.lastname: Ribeiro
authors.affiliation: Department of Dentistry, State University Júlio de Mesquita (UNESP), Rua Humaitá, Centro, 1680, Araraquara 14801-903, São Paulo, Brazil. Electronic address: lgandini@foar.unesp.br.
authors.forename: Luiz Gonzaga
authors.initials: LG
authors.lastname: Gandini Júnior
authors.affiliation: School of Dentistry, University Tiradentes (UNIT), Rua Terêncio Sampaio, 309, Grageru, Aracaju 49025700, Sergipe, Brazil; Laboratory of Morphology and Structural Biology, Science and Technology Institute (ITP), Avenida Murilo Dantas, 300, Prédio do ITP, Farolândia, Aracaju 49032-490, Sergipe, Brazil. Electronic address: ricardo.patologia@uol.com.br.
authors.forename: Ricardo Luiz Cavalcanti
authors.initials: RL
authors.lastname: de Albuquerque-Júnior
mesh_terms: D000539:Alveolar Process / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects*; D000818:Animals; D001808:Blood Vessels / Q000528:radiation effects; D002452:Cell Count; D003094:Collagen / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000532:radiotherapy*; D028022:Low-Level Light Therapy / Q000009:adverse effects*; D008297:Male; D010006:Osteoblasts / Q000473:pathology / Q000528:radiation effects; D010010:Osteoclasts / Q000473:pathology / Q000528:radiation effects; D010513:Periodontal Ligament / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D014087:Tooth Movement Techniques
chemical_list: D003094:Collagen
keywords: Diabetes mellitus; LLLT; Periodontal ligament