title: STAG2 loss of expression is rare in aneuploid malignant   salivary gland neoplasms.
abstract: BACKGROUND
  STAG2 depletion leads to loss of centromere cohesion in vitro, and some human neoplasms have been shown to lose expression of this protein. As a result, STAG2 loss has been shown to cause chromosomal instability and aneuploidy in human cancer cell lines.
  
  
  METHODS
  We tested the hypothesis that aneuploid salivary gland tumours lose immunoexpression of STAG2 compared with diploid tumours using image cytometry to determine DNA ploidy and immunohistochemistry to assess STAG2 protein expression in 30 malignant salivary gland neoplasms.
  
  
  RESULTS
  There was no difference in the immunoexpression of STAG2 between aneuploid (n = 9) and diploid (n = 21) samples. In all but two samples, more than 50% of cells stained for STAG2.
  
  
  CONCLUSIONS
  Aneuploidy in human salivary gland carcinomas is not driven by loss of expression of STAG2.
authors.forename: Carolina Cavaliéri
authors.initials: CC
authors.lastname: Gomes
authors.forename: Vanessa Fátima
authors.initials: VF
authors.lastname: Bernardes
authors.forename: Edward William
authors.initials: EW
authors.lastname: Odell
authors.forename: Ricardo Santiago
authors.initials: RS
authors.lastname: Gomez
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D008949:Adenoma, Pleomorphic / Q000235:genetics / Q000473:pathology; D000328:Adult; D000368:Aged; D000782:Aneuploidy; D034961:Antigens, Nuclear / Q000032:analysis / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics / Q000473:pathology; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000473:pathology; D018277:Carcinoma, Mucoepidermoid / Q000235:genetics / Q000473:pathology; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D043171:Chromosomal Instability / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis; D004171:Diploidy; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D012468:Salivary Gland Neoplasms / Q000737:chemistry / Q000235:genetics*; D000097722:Cohesins
chemical_list: D034961:Antigens, Nuclear; D018797:Cell Cycle Proteins; D004273:DNA, Neoplasm; C508565:STAG2 protein, human; D000097722:Cohesins