title: Inhibition of aminoglycoside 6'-N-acetyltransferase type Ib by zinc: reversal of amikacin resistance in Acinetobacter baumannii and Escherichia coli by a zinc ionophore.
abstract: In vitro activity of the aminoglycoside 6'-N-acetyltransferase type Ib [AAC(6')-Ib] was inhibited by ZnCl2 with a 50% inhibitory concentration (IC50) of 15 μM. Growth of Acinetobacter baumannii or Escherichia coli harboring aac(6')-Ib in cultures containing 8 μg/ml amikacin was significantly inhibited by the addition of 2 μM Zn(2+) in complex with the ionophore pyrithione (ZnPT).
authors.affiliation: Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, California, USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Lin
authors.affiliation: Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, California, USA.
authors.forename: Tung
authors.initials: T
authors.lastname: Tran
authors.affiliation: Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, California, USA.
authors.forename: Jamal Y
authors.initials: JY
authors.lastname: Alam
authors.affiliation: Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, California, USA.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Herron
authors.affiliation: Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, California, USA Instituto de Microbiología y Parasitología Médica (IMPaM, UBA-CONICET), School of Medicine, UBA, Buenos Aires, Argentina.
authors.forename: Maria Soledad
authors.initials: MS
authors.lastname: Ramirez
authors.affiliation: Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, California, USA mtolmasky@fullerton.edu.
authors.forename: Marcelo E
authors.initials: ME
authors.lastname: Tolmasky
mesh_terms: D000123:Acetyltransferases / Q000037:antagonists & inhibitors*; D040981:Acinetobacter baumannii / Q000201:enzymology*; D000583:Amikacin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D004926:Escherichia coli / Q000201:enzymology*; D007476:Ionophores / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011725:Pyridines / Q000494:pharmacology; D013871:Thiones / Q000494:pharmacology; D017967:Zinc Compounds / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D002712:Chlorides; D007476:Ionophores; D011725:Pyridines; D013871:Thiones; D017967:Zinc Compounds; C008704:pyrithione; D000583:Amikacin; C016837:zinc chloride; D000123:Acetyltransferases; C056532:aminoglycoside N(6')-acetyltransferase