title: Synthetic mRNAs for manipulating cellular phenotypes: an overview.
abstract: Availability of high quality synthetic mRNAs (syn-mRNAs) has enabled progress in their applications. Important structural features and quality requirements are discussed. Developments in the application of mRNA-mediated manipulation of cells are presented (i) mRNA-directed expression of antigens in dendritic cells for vaccination projects in oncogenesis, infectious disease and allergy prevention; (ii) reprogramming of human fibroblasts to induced pluripotent stem cells with their subsequent differentiation to the desired cell type; (iii) applications in gene therapy.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts-University Kiel, Arnold-Heller-Str. 3, Building 27, D-24105 Kiel, Germany; Institute of Immunology, Christian-Albrechts-University Kiel, Arnold-Heller-Str. 3, Building 17, D-24105 Kiel, Germany.
authors.forename: Elgar Susanne
authors.initials: ES
authors.lastname: Quabius
authors.affiliation: AmpTec GmbH, Königstr. 4A, 22767 Hamburg, Germany. Electronic address: krupp@amp-tec.com.
authors.forename: Guido
authors.initials: G
authors.lastname: Krupp
mesh_terms: D000818:Animals; D002477:Cells / Q000378:metabolism*; D015316:Genetic Therapy; D006801:Humans; D007111:Immunity, Cellular; D007113:Immunity, Innate; D010641:Phenotype; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger