title: Sphingorhabdus arenilitoris sp. nov., isolated from a coastal sand, and reclassification of Sphingopyxis rigui as Sphingorhabdus rigui comb. nov. and Sphingopyxis wooponensis as Sphingorhabdus wooponensis comb. nov.
abstract: A Gram-stain-negative, strictly aerobic, non-flagellated and rod-shaped bacterial strain, designated GJR-7(T), was isolated from coastal sand of the South Sea of South Korea. Strain GJR-7(T) grew optimally at 30 °C, at pH 7.0-7.5 and without NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJR-7(T) clustered with the type strains of Sphingopyxis wooponensis, Sphingopyxis rigui and Sphingorhabdus planktonica, with which it exhibited 16S rRNA gene sequence similarity values of 96.0-96.3%. Sequence similarities to the type strains of other recognized species were less than 95.5%. Strain GJR-7(T) contained Q-10 as the predominant ubiquinone and C(18 : 1)ω7c, 11-methyl C(18 : 1)ω7c and summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c) or C(14 : 0) 2-OH. The major polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and one unidentified glycolipid. The DNA G+C content of strain GJR-7(T) was 55.5 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, revealed that strain GJR-7(T) is separated from the type strains of Sphingopyxis wooponensis, Sphingopyxis rigui and Sphingorhabdus planktonica. On the basis of the data presented, strain GJR-7(T) is considered to represent a novel species of the genus Sphingorhabdus, for which the name Sphingorhabdus arenilitoris sp. nov. is proposed. The type strain is GJR-7(T) ( = KCTC 42051(T) = CECT 8531(T)). It is also proposed that Sphingopyxis wooponensis and Sphingopyxis rigui should be reclassified as members of the genus Sphingorhabdus.
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Ja-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors.forename: Hyangmi
authors.initials: H
authors.lastname: Kim
authors.affiliation: Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009792:Oceans and Seas; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012822:Silicon Dioxide; C025204:Ubiquinone Q2