title: Effects of sodium selenite on aflatoxin B1-induced decrease of ileac T cell and the mRNA contents of IL-2, IL-6, and TNF-α in broilers.
abstract: The aim of this work was to assess the protective effect of sodium selenite on the ileum mucosal immunologic toxicity induced by aflatoxin B1 (AFB1). One hundred and eighty one-day-old healthy male avian broilers were divided into four groups of three replicates and 15 birds per replicate and fed with basal diet (control group), 0.3 mg/kg AFB1 (AFB1 group), 0.4 mg/kg Se (+Se group), and 0.3 mg/kg AFB1+0.4 mg/kg Se (AFB1+Se group), respectively. The ileac T-cell subsets were determined by the methods of flow cytometry (FCM), and the mRNA contents of interleukin-2 (IL-2), interleukin-6(IL-6), and tumor necrosis factor-alpha (TNF-α) by quantitative real-time PCR. Compared with those in control group, the percentages of CD3+, CD3+CD4+, CD3+CD8+ intraepithelial lymphocytes (IELs) and LPLs, the CD4+/CD8+ ratio of IELs, and the mRNA contents of IL-2, IL-6, and TNF-α were decreased in AFB1 group. However, compared with those in AFB1 group, these parameters of AFB1+Se group were increased to be close to those in control group. It was concluded that 0.3 mg/kg AFB1 could reduce the cellular immune function of the ileum mucosa, but 0.4 mg/kg supplemented dietary selenium showed protective effects on AFB1-induced immunologic injury.
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: He
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Chen
authors.forename: Weimin
authors.initials: W
authors.lastname: Lai
authors.forename: Gang
authors.initials: G
authors.lastname: Shu
authors.forename: Li
authors.initials: L
authors.lastname: Tang
mesh_terms: D016604:Aflatoxin B1 / Q000494:pharmacology*; D000818:Animals; D002645:Chickens; D007082:Ileum / Q000276:immunology*; D007376:Interleukin-2 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger; D018038:Sodium Selenite / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D007376:Interleukin-2; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016604:Aflatoxin B1; D018038:Sodium Selenite