title: A rapid and precise method for quantification of fatty acids in human serum cholesteryl esters by liquid chromatography and tandem mass spectrometry.
abstract: We described a rapid and precise method for simultaneous quantification of eleven fatty acids in human serum cholesteryl esters (CEFAs) by liquid chromatography and tandem mass spectrometry (LC-MS/MS). After extraction of serum lipids with isopropanol, CEFAs were separated on reversed phase liquid chromatography and detected by mass spectrometry in positive ion mode with multiple reaction monitor. Individual CEFA was quantified by peak area normalization method and expressed as molar percent of total CEFAs. The run time was less than 5 min and detection limits were from 0.31 to 14.50 × 10(-5)mmol/L. Recoveries of the CEFAs ranged from 91.85% to 104.83% with a mean of 99.12%. The intra and total CVs for the measurement of CEFAs were 0.87-7.70% and 1.02-7.65%, respectively. This LC-MS/MS method required no internal standards, eliminated natural isotope interferences, and provided reproducible and reliable results for 11 major CEFAs in human serum. This method can be used in monitoring and evaluating dietary fatty acid intake. Additional studies are needed to evaluate the associations between serum CEFAs and cardiovascular disease risk factors.
authors.affiliation: Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China; The Key Laboratory of Geriatrics, Beijing Hospital Institute of Geriatrics, Ministry of Health, Beijing 100730, China.
authors.forename: Songlin
authors.initials: S
authors.lastname: Yu
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital Institute of Geriatrics, Ministry of Health, Beijing 100730, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dong
authors.affiliation: Beijing Hospital National Center for Clinical Laboratories, Ministry of Health, Beijing 100730, China.
authors.forename: Weiyan
authors.initials: W
authors.lastname: Zhou
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital Institute of Geriatrics, Ministry of Health, Beijing 100730, China.
authors.forename: Ruiyue
authors.initials: R
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital Institute of Geriatrics, Ministry of Health, Beijing 100730, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Li
authors.affiliation: Beijing Hospital National Center for Clinical Laboratories, Ministry of Health, Beijing 100730, China.
authors.forename: Haijian
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Beijing Hospital National Center for Clinical Laboratories, Ministry of Health, Beijing 100730, China.
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital Institute of Geriatrics, Ministry of Health, Beijing 100730, China.
authors.forename: Hanbang
authors.initials: H
authors.lastname: Guo
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital Institute of Geriatrics, Ministry of Health, Beijing 100730, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Beijing Hospital National Center for Clinical Laboratories, Ministry of Health, Beijing 100730, China.
authors.forename: Chuanbao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital Institute of Geriatrics, Ministry of Health, Beijing 100730, China; Beijing Hospital National Center for Clinical Laboratories, Ministry of Health, Beijing 100730, China. Electronic address: wchen@bjhmoh.cn.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002318:Cardiovascular Diseases / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood* / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005227:Fatty Acids / Q000097:blood*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012307:Risk Factors; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
chemical_list: D002788:Cholesterol Esters; D005227:Fatty Acids
keywords: Cardiovascular diseases; Cholesteryl ester; Fatty acids; Liquid chromatography and tandem mass spectrometry