title: Decrease of PPARδ in Type-1-Like Diabetic Rat for Higher Mortality after Spinal Cord Injury.
abstract: Changes in the peroxisome proliferator-activated receptors- δ (PPAR δ ) expression in rats after spinal cord injury (SCI) have been previously reported. Diabetic animals show a higher mortality after SCI. However, the relationship between the progress of diabetes and PPAR δ in SCI remains unknown. In the present study, we used compressive SCI in streptozotocin-(STZ-) induced diabetic rats. GW0742, a PPAR δ agonist, was used to evaluate its merit in STZ rats after SCI. Changes in PPAR δ expression were detected by Western blot. Survival rates were also estimated. A lower expression of PPAR δ in spinal cords of STZ-diabetic rats was observed. In addition, the survival times in two-week induction diabetes were longer than those in eight-week induction group, which is consistent with the expression of PPAR δ in the spinal cord. Moreover, GW0742 significantly increased the survival time of STZ rats. Furthermore, their motor function and pain response were attenuated by GSK0660, a selective PPAR δ antagonist, but were enhanced by GW0742. In conclusion, the data suggest that higher mortality rate in STZ-diabetic rats with SCI is associated with the decrease of PPAR δ expression. Thus, change of PPAR δ expression with the progress of diabetes seems responsible for the higher mortality rate after SCI.
authors.affiliation: Department of Neurosurgery, Mackay Memorial Hospital, Taipei City 10449, Taiwan ; Graduate Institute of Injury Prevention and Control, Taipei Medical University, Taipei City 10401, Taiwan.
authors.forename: Cheng-Chia
authors.initials: CC
authors.lastname: Tsai
authors.affiliation: Department of Surgery, Kaohsiung Municipal Hsiao-Kang Hospital and Kaohsiung Medical University, Kaohsiung City 81201, Taiwan.
authors.forename: Kung-Shing
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Chi Mei Medical Center, Yong Kang, Tainan City 71101, Taiwan ; Department of Medical Research, Chi Mei Medical Center, Yong Kang, Tainan City 71101, Taiwan ; Department of Biotechnology, Southern Taiwan University, Yong Kang, Tainan City 71102, Taiwan.
authors.forename: Sheng-Hsien
authors.initials: SH
authors.lastname: Chen
authors.affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan City 70101, Taiwan.
authors.forename: Li-Jen
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Institute of Medical Science, College of Health Science, Chang Jung Christian University, Gui-Ren, Tainan City 71301, Taiwan.
authors.forename: Keng-Fan
authors.initials: KF
authors.lastname: Liu
authors.affiliation: Department of Medical Research, Chi Mei Medical Center, Yong Kang, Tainan City 71101, Taiwan ; Institute of Medical Science, College of Health Science, Chang Jung Christian University, Gui-Ren, Tainan City 71301, Taiwan.
authors.forename: Juei-Tang
authors.initials: JT
authors.lastname: Cheng