title: Storage of apheresis platelet concentrates after manual replacement of >95% of plasma with PAS 5.
abstract: OBJECTIVE
  Recently, a glucose- and bicarbonate-containing additive solution termed PAS 5 demonstrated acceptable 7-day platelet storage after >95% plasma replacement with PAS on the day of collection (Day 0). In this study, we examined platelet storage in >95% PAS 5 after manual washing of Day 1 apheresis platelets in plasma collected using either the Amicus or Trima plateletpheresis devices.
  
  
  METHODS
  Triple platelet donations in plasma were obtained from Amicus (n = 10) and Trima (n = 10) plateletpheresis devices and stored overnight before being centrifuged and manually processed into three units with the following storage media: 100% plasma, >95% PAS 5 or 65% PAS 5/35% plasma. Platelet units were sampled on Days 1, 5 and 7 of storage using a range of tests recommended by the UK guidelines.
  
  
  RESULTS
  The majority of in vitro assay results for platelets in PAS 5 were similar to results in paired 100% plasma platelets (controls). The pH of PAS 5 stored platelet units was above the UK recommended guidelines of 7·4 by Day 5. PAS 5 platelets were no more activated than controls as evidenced by comparable soluble P-selectin levels and CD62p and CD42b expression. PAS 5 platelets also exhibited adhesion and aggregation profiles higher than (Day 1) or comparable to (Days 5 and 7) controls as measured by Impact R.
  
  
  CONCLUSIONS
  The 7-day in vitro storage parameters investigated were comparable between >95% PAS 5 and 100% plasma platelets derived from both Amicus and Trima plateletpheresis devices, with the exception that lactose dehydrogenase release rate and pH were significantly higher in PAS 5 units.
authors.affiliation: National Science Laboratory, Scottish National Blood Transfusion Service, Microbiology and Components RD&I Group, Edinburgh, UK.
authors.forename: A
authors.initials: A
authors.lastname: Morrison
authors.forename: L
authors.initials: L
authors.lastname: McMillan
authors.forename: K
authors.initials: K
authors.lastname: Radwanski
authors.forename: O
authors.initials: O
authors.lastname: Blatchford
authors.forename: K
authors.initials: K
authors.lastname: Min
authors.forename: J
authors.initials: J
authors.lastname: Petrik
mesh_terms: D001782:Blood Donors; D001792:Blood Platelets; D001793:Blood Preservation; D006801:Humans; D010983:Plateletpheresis; D012996:Solutions; D006113:United Kingdom
chemical_list: D012996:Solutions
keywords: Amicus; Trima; apheresis platelets; platelet additive solution