title: Synthesis of calcium phosphate-zirconia scaffold and human endometrial adult stem cells for bone tissue engineering.
abstract: To address the hypothesis that using a zirconia (ZrO2)/ β-tricalcium phosphate (β-TCP) composite might improve both the mechanical properties and cellular compatibility of the porous material, we fabricated ZrO2/β-TCP composite scaffolds with different ZrO2/β-TCP ratios, and evaluated their physical and mechanical characteristics, also the effect of three-dimensional (3D) culture (ZrO2/β-TCP scaffold) on the behavior of human endometrial stem cells. Results showed the porosity of a ZrO2/β-TCP scaffold can be adjusted from 65% to 84%, and the compressive strength of the scaffold increased from 4.95 to 6.25 MPa when the ZrO2 content increased from 30 to 50 wt%. The cell adhesion and proliferation in the ZrO2/β-TCP scaffold was greatly improved when ZrO2 decreased. Moreover, in vitro study showed that an osteoblasts-loaded ZrO2/β-TCP scaffold provided a suitable 3D environment for osteoblast survival and enhanced bone regeneration. We thus showed that a porous ZrO2/β-TCP composite scaffold has excellent mechanical properties, and cellular/tissue compatibility, and would be a promising substrate to achieve both bone reconstruction and regeneration needed during in vivo study for treatment of large bone defects.
authors.affiliation: a Department of Tissue Engineering , School of Advanced Technologies in Medicine, Tehran University of Medical Sciences , Tehran , Iran.|c Transplant Research Center, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Alizadeh
authors.affiliation: b Department of Biomedical Engineering , Amirkabir University of Technology , Tehran , Iran.
authors.forename: Fathollah
authors.initials: F
authors.lastname: Moztarzadeh
authors.affiliation: a Department of Tissue Engineering , School of Advanced Technologies in Medicine, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Seyed Naser
authors.initials: SN
authors.lastname: Ostad
authors.affiliation: a Department of Tissue Engineering , School of Advanced Technologies in Medicine, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Azami
authors.affiliation: c Transplant Research Center, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Bita
authors.initials: B
authors.lastname: Geramizadeh
authors.affiliation: d Department of Parasitology , Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Hatam
authors.affiliation: b Department of Biomedical Engineering , Amirkabir University of Technology , Tehran , Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Bizari
authors.affiliation: a Department of Tissue Engineering , School of Advanced Technologies in Medicine, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Tavangar
authors.affiliation: a Department of Tissue Engineering , School of Advanced Technologies in Medicine, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Vasei
authors.affiliation: a Department of Tissue Engineering , School of Advanced Technologies in Medicine, Tehran University of Medical Sciences , Tehran , Iran.|e Brain and Spinal Injury Research Center, Imam Hospital, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Ai
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D001672:Biocompatible Materials; D001861:Bone Regeneration; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D016062:Porosity; D046509:Tissue Culture Techniques; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D015040:Zirconium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D015040:Zirconium; C028541:zirconium oxide
keywords: bone tissue engineering; endometrial stem cells; porous scaffold; zirconia; β-tricalcium phosphate