title: Characterization of anticancer, DNase and antifungal activity of pumpkin 2S albumin.
abstract: The plant 2S albumins exhibit a spectrum of biotechnologically exploitable functions. Among them, pumpkin 2S albumin has been shown to possess RNase and cell-free translational inhibitory activities. The present study investigated the anticancer, DNase and antifungal activities of pumpkin 2S albumin. The protein exhibited a strong anticancer activity toward breast cancer (MCF-7), ovarian teratocarcinoma (PA-1), prostate cancer (PC-3 and DU-145) and hepatocellular carcinoma (HepG2) cell lines. Acridine orange staining and DNA fragmentation studies indicated that cytotoxic effect of pumpkin 2S albumin is mediated through induction of apoptosis. Pumpkin 2S albumin showed DNase activity against both supercoiled and linear DNA and exerted antifungal activity against Fusarium oxysporum. Secondary structure analysis by CD showed that protein is highly stable up to 90°C and retains its alpha helical structure. These results demonstrated that pumpkin 2S albumin is a multifunctional protein with host of potential biotechnology applications.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
authors.forename: Prabhat Pratap Singh
authors.initials: PP
authors.lastname: Tomar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
authors.forename: Kumar
authors.initials: K
authors.lastname: Nikhil
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
authors.forename: Anamika
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
authors.forename: Purushotham
authors.initials: P
authors.lastname: Selvakumar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
authors.forename: Partha
authors.initials: P
authors.lastname: Roy
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India. Electronic address: aksbsfbs@yahoo.co.in.
authors.forename: Ashwani Kumar
authors.initials: AK
authors.lastname: Sharma
mesh_terms: D055516:2S Albumins, Plant / Q000737:chemistry* / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001709:Biotechnology; D045744:Cell Line, Tumor; D028464:Cucurbita / Q000737:chemistry*; D053938:DNA Fragmentation / Q000187:drug effects; D003851:Deoxyribonucleases / Q000737:chemistry* / Q000494:pharmacology*; D005260:Female; D005670:Fusarium / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D008297:Male; D055550:Protein Stability; D017433:Protein Structure, Secondary
chemical_list: D055516:2S Albumins, Plant; D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D003851:Deoxyribonucleases
keywords: Anticancer activity; Antifungal activity; CD studies; Deoxyribonuclease activity; Pumpkin 2S albumin