title: Generation of homologous cell pairs using the oral lymphatic system.
abstract: The purpose of this study is to establish in vivo and in vitro models for studying lymphatic metastasis of squamous cell carcinoma (SCC). Three cell lines CAL-27, Tca-83, and HeLa were injected into the tongue of nude mice. Forty days after injection, we could isolate cells of 2 homologous cell lines LN-CAL-27 and LN-HeLa from lymph node metastasis lesions. Then, the homologous cell pairs were compared by the CCK-8 assay, wound healing assay, real-time PCR, western blot, and animal experiments. The results showed that all the three cell lines could be used to establish lymphatic metastasis animal models, and the lymphatic metastasis process was observed clearly. In addition, the homologous cell pairs performed differently from parent lines with respect to biological behavior and lymphatic metastasis-related gene and protein expression. In conclusion, CAL-27, Tca-83, and HeLa cells could be used to simulate the lymphatic metastasis process of oral cancer in vivo. Furthermore, the homologous cell pairs (CAL-27 and LN-CAL-27; HeLa and LN-HeLa) are potential tools for in vitro investigation of the mechanisms underlying metastasis.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology Beijing, China.
authors.forename: Yin-Fei
authors.initials: YF
authors.lastname: Pu
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology Beijing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology Beijing, China.
authors.forename: Huan-Huan
authors.initials: HH
authors.lastname: Wu
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology Beijing, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Bian
authors.affiliation: Department of Pathology, Peking University School and Hospital of Stomatology Beijing, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Hong
authors.affiliation: Central Laboratory, Peking University School and Hospital of Stomatology Beijing, China.
authors.forename: Yi-Xiang
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology Beijing, China.
authors.forename: Chuan-Bin
authors.initials: CB
authors.lastname: Guo
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D014062:Tongue Neoplasms / Q000235:genetics / Q000473:pathology*; D059467:Transcriptome; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology*
keywords: Oral cancer; animal model; homologous cell pair; lymphatic metastasis; oral lymphatic system