title: Production of novel "functional oil" rich in diglycerides and phytosterol esters with "one-pot" enzymatic transesterification.
abstract: Diglycerides and phytosterol esters are two important functional lipids. Phytosterol esters mixed with dietary diglyceride could not only influence body weight but also prevent or reverse insulin resistance and hyperlipidemia. In this study, a kind of novel "functional oil" rich in both diglycerides and phytosterol esters was prepared with "one-pot" enzymatic transesterification. First, lipase AYS (Candida rugosa) was immobilized on the porous cross-linked polystyrene resin beads (NKA) via hydrophobic interaction. The resulting immobilized AYS showed much better transesterification activity and thermal stability to freeways. On the basis of the excellent biocatalyst prepared, a method for high-efficiency enzymatic esterification of phytosterols with different triglycerides to produce corresponding functional oils rich in both diglycerides and phytosterol esters was developed. Four functional oils rich in both diglycerides and phytosterol esters with conversions >92.1% and controllable fatty acid composition were obtained under the optimized conditions: 80 mmol/L phytosterols, 160 mmol/L triglycerides, and 25 mg/mL AYS@NKA at 180 rpm and 50 °C for 12 h in hexane. The prepared functional oil possessed low acid value (≤1.0 mgKOH/g), peroxide value (≤2.1 mmol/kg), and conjugated diene value (≤1.96 mmol/kg) and high diglyceride and phytosterol ester contents (≥10.4 and ≥20.2%, respectively). All of the characteristics favored the wide application of the functional oil in different fields of functional food.
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Oilcrops Lipid Chemistry and Nutrition, Wuhan 430062, China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Zheng
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.forename: Feng-Hong
authors.initials: FH
authors.lastname: Huang
authors.forename: Ping-Mei
authors.initials: PM
authors.lastname: Guo
authors.forename: Xia
authors.initials: X
authors.lastname: Xiang
authors.forename: Qian-Chun
authors.initials: QC
authors.lastname: Deng
authors.forename: Wen-Lin
authors.initials: WL
authors.lastname: Li
authors.forename: Chu-Yun
authors.initials: CY
authors.lastname: Wan
authors.forename: Chang
authors.initials: C
authors.lastname: Zheng
mesh_terms: D002175:Candida / Q000201:enzymology*; D004075:Diglycerides / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D004951:Esterification; D004952:Esters / Q000737:chemistry*; D055951:Functional Food / Q000032:analysis; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D010840:Phytosterols / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D000074242:Sunflower Oil; D014280:Triglycerides / Q000737:chemistry
chemical_list: D004075:Diglycerides; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D010840:Phytosterols; D010938:Plant Oils; D000074242:Sunflower Oil; D014280:Triglycerides; D008049:Lipase