title: Improved human mesenchymal stem cell isolation.
abstract: Human mesenchymal stem cells (hMSCs) are currently available for a range of applications and benefits and have become a good material for regenerative medicine, tissue engineering, and disease therapy. Before ex vivo expansion, isolation and characterization of primary hMSCs from peripheral tissues are key steps for obtaining adequate materials for clinical application. The proportion of peripheral stem cells is very low in surrounding tissues and organs; thus the recovery ratio will be a limiting factor. In this review, we summarized current common methods used to isolate peripheral stem cells, as well as the new insights revealed to improve the quantity of stem cells and their stemness. These strategies offer alternative ways to acquire hMSCs in a convenient and/or effective manner, which is important for clinical treatments. Improved isolation and mass amplification of the hMSCs while ensuring their stemness and quantity will be an important step for clinical use. Enlarged suitable hMSCs are more clinically applicable for therapeutic transplants and may help people live longer and better.
authors.affiliation: Center for Neuropsychiatry, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Tzu-Min
authors.initials: TM
authors.lastname: Chan
authors.forename: Horng-Jyh
authors.initials: HJ
authors.lastname: Harn
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Lin
authors.forename: Pei-Wen
authors.initials: PW
authors.lastname: Chou
authors.forename: Julia Yi-Ru
authors.initials: JY
authors.lastname: Chen
authors.forename: Tsung-Jung
authors.initials: TJ
authors.lastname: Ho
authors.forename: Tzyy-Wen
authors.initials: TW
authors.lastname: Chiou
authors.forename: Hong-Meng
authors.initials: HM
authors.lastname: Chuang
authors.forename: Shao-Chih
authors.initials: SC
authors.lastname: Chiu
authors.forename: Yen-Chung
authors.initials: YC
authors.lastname: Chen
authors.forename: Ssu-Yin
authors.initials: SY
authors.lastname: Yen
authors.forename: Mao-Hsuan
authors.initials: MH
authors.lastname: Huang
authors.forename: Bing-Chiang
authors.initials: BC
authors.lastname: Liang
authors.forename: Shinn-Zong
authors.initials: SZ
authors.lastname: Lin
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D001854:Bone Marrow Cells / Q000166:cytology; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism