title: Localization and interaction of hydroxyflavones with lipid bilayer model membranes: a study using DSC and multinuclear NMR.
abstract: The localization and interaction of six naturally occurring flavones (FLV, 5HF, 6HF, 7HF, CHY and BLN) in DPPC bilayers were studied using DSC and multi-nuclear NMR. DSC results indicate that FLV and 6HF interact with alkyl chains. The (1)H NMR shows interaction of flavones with the sn-glycero region. Ring current induced chemical shifts indicate that 6HF and BLN acquire parallel orientation in bilayers. 2D NOESY spectra indicate partitioning of the B-ring into the alkyl chain region. The DSC, NMR and binding studies indicate that 5HF and 7HF are located near head group region, while 6HF, CHY and BLN are located in the vicinity of sn-glycero region, and FLV is inserted deepest in the membrane.
authors.affiliation: National Facility for High Field NMR, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India.
authors.forename: Ragini
authors.initials: R
authors.lastname: Sinha
authors.affiliation: Principal K.M. Kundnani College of Pharmacy, Cuffe Parade, Mumbai 400 005, India.
authors.forename: Akshada
authors.initials: A
authors.lastname: Joshi
authors.affiliation: Principal K.M. Kundnani College of Pharmacy, Cuffe Parade, Mumbai 400 005, India. Electronic address: urmilajjoshi@hotmail.com.
authors.forename: Urmila J
authors.initials: UJ
authors.lastname: Joshi
authors.affiliation: National Facility for High Field NMR, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India.
authors.forename: Sudha
authors.initials: S
authors.lastname: Srivastava
authors.affiliation: National Facility for High Field NMR, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India.
authors.forename: Girjesh
authors.initials: G
authors.lastname: Govil
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D047309:Flavones / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D006801:Humans; D006878:Hydroxides / Q000737:chemistry; D008051:Lipid Bilayers / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010851:Picrates / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D001713:Biphenyl Compounds; D047309:Flavones; D016166:Free Radical Scavengers; D006878:Hydroxides; D008051:Lipid Bilayers; D010851:Picrates; C031356:hydroxide ion; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: CSA; Chemical shifts; DPPC; DSC; Flavones; NMR