title: Effects of molten-salt/ionic-liquid mixture on extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101.
abstract: In this study, lipid extraction from Aurantiochytrium sp. was performed using a molten-salt/ionic-liquid mixture. The total fatty acid content of Aurantiochytrium sp. was 478.8 mg/g cell, from which 145 mg/g cell (30.3% of total fatty acids) of docosahexaenoic acid (DHA) was obtained. FeCl3·6H2O showed a high lipid extraction yield (207.9 mg/g cell), when compared with that of [Emim]OAc, which was only 118.1 mg/g cell; notably however, when FeCl3·6H2O was mixed with [Emim]OAc (5:1, w/w), the yield was increased to 478.6 mg/g cell. When lipid was extracted by the FeCl3·6H2O/[Emim]OAc mixture at a 5:1 (w/w) blending ratio under 90 °C, 30 min reaction conditions, the fatty acid content of the extracted lipid was a high purity 997.7 mg/g lipid, with most of the DHA having been extracted (30.2% of total fatty acids). Overall, lipid extraction from Aurantiochytrium sp. was enhanced by the synergistic effects of the molten-salt/ionic-liquid mixture with different ions.
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon, 305-343, Korea.
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Choi
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Jung
authors.forename: Kyochan
authors.initials: K
authors.lastname: Kim
authors.forename: Jong-Hee
authors.initials: JH
authors.lastname: Kwon
authors.forename: Jin-Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Kim
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Park
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Yang
mesh_terms: D057005:Bioengineering; D056804:Biofuels; D002712:Chlorides; D004281:Docosahexaenoic Acids / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D005290:Ferric Compounds; D005516:Food Microbiology; D007093:Imidazoles; D007477:Ions; D008055:Lipids / Q000302:isolation & purification*; D058086:Microalgae / Q000737:chemistry; D012997:Solvents; D058009:Stramenopiles / Q000737:chemistry*
chemical_list: D056804:Biofuels; D002712:Chlorides; D005227:Fatty Acids; D005290:Ferric Compounds; D007093:Imidazoles; D007477:Ions; D008055:Lipids; D012997:Solvents; D004281:Docosahexaenoic Acids; C518739:1-ethyl-3-methylimidazolium; C024555:ferric chloride