title: Serum level of antibody against benzo[a]pyrene-7,8-diol-9,10-epoxide-DNA adducts in people dermally exposed to PAHs.
abstract: Some specific antibodies indicate the presence of antigenic structures on DNA (DNA adducts) that can play an important role in the process of mutagenesis and/or carcinogenesis. They indicate the presence of increased genotoxic potential (hazard) prior to the formation of disease (primary prevention). The present study was focused on the serum level of benzo[a]pyrene 7,8-diol-9,10-epoxide-DNA adducts antibodies (anti-BPDE-DNA) in psoriatic patients (n = 55) dermally exposed to different levels of polycyclic aromatic hydrocarbons (PAHs). The general goal of the study was to contribute to better understanding of the value of the assumed biomarker (anti-BPDE-DNA) for evaluation of the organism's answer to genotoxic exposure to PAHs. Elevated level of exposure to PAHs resulted in the increased level of anti-BPDE-DNA. However, almost all levels of anti-BPDE-DNA ranged within the field of low values. Both variants of GT (CCT-3% and CCT-5%) induced higher expression of anti-BPDE-DNA in the group of nonsmokers. Significant relations between the level of anti-BPDE-DNA and PASI score, total duration of the therapy, or time of UVR exposure were not found. Further studies are needed to reduce interpretation uncertainty of this promising bioindicator.
authors.affiliation: Institute of Pathological Physiology, Charles University in Prague, Faculty of Medicine in Hradec Kralove, 50038 Hradec Kralove, Czech Republic.
authors.forename: Lenka
authors.identifier: 0000-0002-8580-1485
authors.initials: L
authors.lastname: Borska
authors.affiliation: Institute of Clinical Immunology and Allergology, Charles University in Prague, Faculty of Medicine in Hradec Kralove, 50038 Hradec Kralove, Czech Republic.
authors.forename: Ctirad
authors.initials: C
authors.lastname: Andrys
authors.affiliation: Institute of Clinical Immunology and Allergology, Charles University in Prague, Faculty of Medicine in Hradec Kralove, 50038 Hradec Kralove, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Krejsek
authors.affiliation: Institute of Clinical Biochemistry and Diagnosis, Charles University in Prague, Faculty of Medicine in Hradec Kralove, 50038 Hradec Kralove, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Palicka
authors.affiliation: Institute of Clinical Biochemistry and Diagnosis, Charles University in Prague, Faculty of Medicine in Hradec Kralove, 50038 Hradec Kralove, Czech Republic.
authors.forename: Marcela
authors.initials: M
authors.lastname: Chmelarova
authors.affiliation: Clinic of Dermal and Venereal Diseases, University Hospital Hradec Kralove, 50005 Hradec Kralove, Czech Republic.
authors.forename: Kvetoslava
authors.initials: K
authors.lastname: Hamakova
authors.affiliation: Institute of Pathological Physiology, Charles University in Prague, Faculty of Medicine in Hradec Kralove, 50038 Hradec Kralove, Czech Republic.
authors.forename: Jan
authors.identifier: 0000-0001-8641-4287
authors.initials: J
authors.lastname: Kremlacek
authors.affiliation: Institute of Pathological Physiology, Charles University in Prague, Faculty of Medicine in Hradec Kralove, 50038 Hradec Kralove, Czech Republic.
authors.forename: Pavel
authors.identifier: 0000-0001-5253-2808
authors.initials: P
authors.lastname: Borsky
authors.affiliation: Institute of Hygiene and Preventive Medicine, Charles University in Prague, Faculty of Medicine in Hradec Kralove, 50038 Hradec Kralove, Czech Republic.
authors.forename: Zdenek
authors.identifier: 0000-0001-9978-5757
authors.initials: Z
authors.lastname: Fiala
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D000293:Adolescent; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D003033:Coal Tar / Q000627:therapeutic use; D018736:DNA Adducts / Q000097:blood* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D011565:Psoriasis / Q000097:blood* / Q000276:immunology / Q000473:pathology / Q000628:therapy; D012867:Skin / Q000187:drug effects* / Q000276:immunology; D012907:Smoking / Q000097:blood; D014467:Ultraviolet Therapy
chemical_list: D000906:Antibodies; D015415:Biomarkers; D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; C043931:benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D003033:Coal Tar