title: Preeclampsia enhances neuroglial marker expression in umbilical cord Wharton's jelly-derived mesenchymal stem cells.
abstract: OBJECTIVE
  The aim of the study was to compare the neuroglial phenotype of Wharton's jelly-derived mesenchymal stem cells (WJ-MSC) from pregnancies complicated with preeclampsia and gestational age (GA)-matched controls.
  
  
  METHODS
  WJ-MSC were isolated from umbilical cords from both groups and analyzed for the cell surface expression of MSC markers and the gene and protein expression of neuroglial markers.
  
  
  RESULTS
  All WJ cells were highly positive for the MSC markers CD105, CD90 and CD73, but negative for markers specific for hematopoietic (CD34) and immunological cells (CD45, CD14, CD19 and HLA-DR). WJ-MSC from both groups expressed neuroglial markers (MAP-2, GFAP, MBP, Musashi-1 and Nestin) at the mRNA and protein level. The protein expressions of neuronal (MAP-2) and oligodendrocytic (MBP) markers were significantly increased in WJ-MSC from preeclampsia versus GA-matched controls.
  
  
  CONCLUSIONS
  WJ-MSC from preeclamptic patients are possibly more committed to neuroglial differentiation through the activation of pathways involved both in the pathophysiology of the disease and in neurogenesis.
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital Bern , Bern , Switzerland and.
authors.forename: Marianne
authors.initials: M
authors.lastname: Joerger-Messerli
authors.forename: Esther
authors.initials: E
authors.lastname: Brühlmann
authors.forename: Anice
authors.initials: A
authors.lastname: Bessire
authors.forename: Anna
authors.initials: A
authors.lastname: Wagner
authors.forename: Martin
authors.initials: M
authors.lastname: Mueller
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Surbek
authors.forename: Andreina
authors.initials: A
authors.lastname: Schoeberlein
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D009457:Neuroglia / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D014470:Umbilical Cord / Q000378:metabolism / Q000473:pathology; D059631:Wharton Jelly / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
chemical_list: D015415:Biomarkers
keywords: Mesenchymal stem cells; Wharton's jelly; neuroglial differentiation; perinatal brain injury; preeclampsia