title: Extracellular matrix proteins expression profiling in chemoresistant variants of the A2780 ovarian cancer cell line.
abstract: Ovarian cancer is the leading cause of death among gynaecological malignancies. Extracellular matrix (ECM) can affect drug resistance by preventing the penetration of the drug into cancer cells and increased resistance to apoptosis. This study demonstrates alterations in the expression levels of ECM components and related genes in cisplatin-, doxorubicin-, topotecan-, and paclitaxel-resistant variants of the A2780 ovarian cancer cell line. Affymetrix Gene Chip Human Genome Array Strips were used for hybridisations. The genes that had altered expression levels in drug-resistant sublines were selected and filtered by scatter plots. The genes that were up- or downregulated more than fivefold were selected and listed. Among the investigated genes, 28 genes were upregulated, 10 genes were downregulated, and two genes were down- or upregulated depending on the cell line. Between upregulated genes 12 were upregulated very significantly--over 20-fold. These genes included COL1A2, COL12A1, COL21A1, LOX, TGFBI, LAMB1, EFEMP1, GPC3, SDC2, MGP, MMP3, and TIMP3. Four genes were very significantly downregulated: COL11A1, LAMA2, GPC6, and LUM. The expression profiles of investigated genes provide a preliminary insight into the relationship between drug resistance and the expression of ECM components. Identifying correlations between investigated genes and drug resistance will require further analysis.
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, Poland Święcickiego 6 Street, 61-781 Poznan, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Januchowski
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, Poland Święcickiego 6 Street, 61-781 Poznan, Poland ; Departament of Anatomy, Poznan University of Medical Sciences, Poland Święcickiego 6 Street, 61-781 Poznan, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Zawierucha
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, Poland Święcickiego 6 Street, 61-781 Poznan, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Ruciński
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, Poland Święcickiego 6 Street, 61-781 Poznan, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, Poland Święcickiego 6 Street, 61-781 Poznan, Poland ; Department of Histology and Embryology, Wroclaw Medical University, Poland Chałubińskiego 6A Street, 50-368 Wroclaw, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
mesh_terms: D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D016326:Extracellular Matrix Proteins; D009363:Neoplasm Proteins