title: An antimethanogenic nutritional intervention in early life of ruminants modifies ruminal colonization by Archaea.
abstract: The aim of this work was to study whether feeding a methanogen inhibitor from birth of goat kids and their does has an impact on the archaeal population colonizing the rumen and to what extent the impact persists later in life. Sixteen goats giving birth to two kids were used. Eight does were treated (D+) with bromochloromethane after giving birth and over 2 months. The other 8 goats were not treated (D-). One kid per doe in both groups was treated with bromochloromethane (k+) for 3 months while the other was untreated (k-), resulting in four experimental groups: D+/k+, D+/k-, D-/k+, and D-/k-. Rumen samples were collected from kids at weaning and 1 and 4 months after (3 and 6 months after birth) and from does at the end of the treating period (2 months). Pyrosequencing analyses showed a modified archaeal community composition colonizing the rumen of kids, although such effect did not persist entirely 4 months after; however, some less abundant groups remained different in treated and control animals. The different response on the archaeal community composition observed between offspring and adult goats suggests that the competition occurring in the developing rumen to occupy different niches offer potential for intervention.
authors.affiliation: INAN, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: Leticia
authors.initials: L
authors.lastname: Abecia
authors.affiliation: IBERS, Aberystwyth University, Aberystwyth SY23 3DA, UK.
authors.forename: Kate E
authors.initials: KE
authors.lastname: Waddams
authors.affiliation: INAN, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Martínez-Fernandez
authors.affiliation: INAN, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: A Ignacio
authors.initials: AI
authors.lastname: Martín-García
authors.affiliation: INAN, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Ramos-Morales
authors.affiliation: IBERS, Aberystwyth University, Aberystwyth SY23 3DA, UK.
authors.forename: C Jamie
authors.initials: CJ
authors.lastname: Newbold
authors.affiliation: INAN, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: David R
authors.initials: DR
authors.lastname: Yáñez-Ruiz
mesh_terms: D000818:Animals; D001105:Archaea / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D004032:Diet / Q000379:methods*; D006041:Goats; D006846:Hydrocarbons, Halogenated / Q000008:administration & dosage*; D008137:Longitudinal Studies; D012417:Rumen / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D006846:Hydrocarbons, Halogenated; C006811:bromochloromethane