title: Huaier suppresses proliferation and induces apoptosis in human pulmonary cancer cells via upregulation of miR-26b-5p.
abstract: Various studies have reported that Huaier possesses anti-tumor effects. However, the mechanisms are not completely elucidated. Here, we found 66 differentially expressed miRNAs in Huaier-treated pulmonary adenocarcinoma A549 cells, with upregulation of miR-26b-5p. Transfection of A549 cells with miR-26b-5p mimic inhibited proliferation and induced apoptosis, while transfection of Huaier-treated A549 cells with a miR-26b-5p inhibitor reversed the effects of Huaier. EZH2 was verified as the target of miR-26b-5p. Thus, our findings indicate that Huaier might suppress proliferation and induce apoptosis in lung cancer cells via a miR-26b-5p-EZH2-mediated approach, which provides a new perspective for understanding the anti-tumor effects of Huaier.
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China; School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China.
authors.forename: Tangwei
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China; Department of Central Laboratory, Central Hospital of Wuhan, Wuhan 430014, China; Cancer Research Institute of Wuhan, Wuhan 430014, China.
authors.forename: Weiqun
authors.initials: W
authors.lastname: Chen
authors.affiliation: Cancer Research Institute of Wuhan, Wuhan 430014, China.
authors.forename: Shuiyi
authors.initials: S
authors.lastname: Liu
authors.affiliation: Cancer Research Institute of Wuhan, Wuhan 430014, China; Department of Oncology, Central Hospital of Wuhan, Wuhan 430014, China.
authors.forename: Hongda
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.
authors.forename: Deyong
authors.initials: D
authors.lastname: Kong
authors.affiliation: Department of Central Laboratory, Central Hospital of Wuhan, Wuhan 430014, China; Cancer Research Institute of Wuhan, Wuhan 430014, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Central Laboratory, Central Hospital of Wuhan, Wuhan 430014, China; Cancer Research Institute of Wuhan, Wuhan 430014, China; Department of Oncology, Central Hospital of Wuhan, Wuhan 430014, China.
authors.forename: Qinzhi
authors.initials: Q
authors.lastname: Kong
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China. Electronic address: ningyong128@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ning
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China; Department of Central Laboratory, Central Hospital of Wuhan, Wuhan 430014, China; Cancer Research Institute of Wuhan, Wuhan 430014, China. Electronic address: lzx71@yahoo.com.
authors.forename: Zhongxin
authors.initials: Z
authors.lastname: Lu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D000071221:Enhancer of Zeste Homolog 2 Protein; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D063151:Polycomb Repressive Complex 2 / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D055454:Trametes / Q000737:chemistry* / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects*; D051176:beta Catenin / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D000071221:Enhancer of Zeste Homolog 2 Protein; D035683:MicroRNAs; D063151:Polycomb Repressive Complex 2; D019253:Proto-Oncogene Proteins c-bcl-2; D051176:beta Catenin; C528163:MIRN26 microRNA, human; C498409:EZH2 protein, human
keywords: Apoptosis; EZH2; Huaier; Lung cancer; Proliferation; miR-26b-5p