title: Purification, crystallization and preliminary crystallographic analysis of a ribosome-recycling factor from Thermoanaerobacter tengcongensis (TteRRF).
abstract: Ribosome-recycling factor (RRF) plays an essential role in the fourth step of protein synthesis in prokaryotes. RRF combined with elongation factor G (EF-G) disassembles the post-termination ribosome complex and recycles the protein synthesis machine for the next round of translation. A reductive-methylation-modified RRF from Thermoanaerobacter tengcongensis (TteRRF) has been crystallized using the vapour-diffusion method. The crystal grew in a condition consisting of 0.1 M citric acid pH 3.5, 3.0 M NaCl and 50 mg ml(-1) methylated protein solution at 289 K. A complete data set was collected from a crystal to 2.80 Å resolution using synchrotron radiation at 100 K. The crystal belonged to space group P6122/P6522 with unit-cell parameters a = b = 103.26, c = 89.17 Å. The asymmetric unit was estimated to contain one molecule of TteRRF.
authors.affiliation: College of Science, Beijing Forestry University, 35 Qinghuadong Road, Haidian District, Beijing 100083, People's Republic of China.
authors.forename: Guijun
authors.initials: G
authors.lastname: Shang
authors.affiliation: College of Science, Beijing Forestry University, 35 Qinghuadong Road, Haidian District, Beijing 100083, People's Republic of China.
authors.forename: Duo
authors.initials: D
authors.lastname: Feng
authors.affiliation: College of Science, Beijing Forestry University, 35 Qinghuadong Road, Haidian District, Beijing 100083, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Lu
authors.affiliation: Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Science, Beijing Forestry University, 35 Qinghuadong Road, Haidian District, Beijing 100083, People's Republic of China.
authors.forename: Huaixing
authors.initials: H
authors.lastname: Cang
authors.affiliation: College of Science, Beijing Forestry University, 35 Qinghuadong Road, Haidian District, Beijing 100083, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Ruchang
authors.initials: R
authors.lastname: Bi
mesh_terms: D003460:Crystallization; D003461:Crystallography; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012270:Ribosomes; D046968:Thermoanaerobacter; D014961:X-Ray Diffraction
chemical_list: D012269:Ribosomal Proteins; C087267:ribosome releasing factor
keywords: Thermoanaerobacter tengcongensis; ribosome-recycling factor