title: PCR and microscopic identification of isolated Leishmania tropica from clinical samples of cutaneous leishmaniasis in human population of Kohat region in Khyber Pakhtunkhwa.
abstract: Leishmania tropica was isolated from the clinical patients of cutaneous leishmaniasis in rural community of Kohat district in Khyber Pakhtunkhwa province and was identified through PCR, microscopy, and culture techniques. A total of 113 samples from the clinical patients were examined through PCR, microscopy, and culture which showed 87.61% (99/113), 53.98% (61/113), and 46.90% (53/113) prevalence. During the study, 186 bp Leishmania tropica was identified through PCR. Thus the sensitivity of PCR is very high as compared to the conventional techniques.
authors.affiliation: Arriyadh Community College, King Saud University, P.O. Box 28095, Riyadh 11437, Saudi Arabia.
authors.forename: Nasser M
authors.initials: NM
authors.lastname: Abd El-Salam
authors.affiliation: Department of Zoology, Kohat University of Science and Technology, Kohat 26000, Pakistan.
authors.forename: Sultan
authors.initials: S
authors.lastname: Ayaz
authors.affiliation: Department of Chemistry, Government College Ara Khel, FR Kohat, Khyber Pakhtunkhwa 26000, Pakistan.
authors.forename: Riaz
authors.initials: R
authors.lastname: Ullah
mesh_terms: D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D019353:Endemic Diseases; D006801:Humans; D007895:Leishmania tropica / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000469:parasitology* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D016054:DNA, Protozoan