title: Prostate anatomy in motheaten viable (me(v)) mice with mutations in the protein tyrosine phosphatase SHP-1.
abstract: OBJECTIVE
  To study prostate and seminal vesicle anatomy in viable motheaten (mev) with mutations in PTPN6 gene leading to a severe reduction in the activity of protein tyrosine phosphatase SHP-1. Homozygous mev mice exhibit multiple anomalies that include immunodeficiencies, increased proliferation of macrophage, neutrophil, and erythrocyte progenitors, decreased bone density and sterility.
  
  
  METHODS
  We analyzed macro- and microscopic anatomy of the seminal vesicle and prostate macro- and microscopic anatomy of 5 mev/mev and 8 wt/wt adult 7 week old mice. Computerized morphometric analysis was performed to measure the relative changes appearing in the epithelial volume of the different prostatic lobes.
  
  
  RESULTS
  All mice studied revealed normal genital organs (penis, testis, epididymis, vas deferens) and bladder. The seminal vesicle was absent in all mev/mev individuals analyzed, being normal and very noticeable in wt/wt mice. The different glands that compose the prostatic complex (anterior, ventral and dorso-lateral prostate) were atrophied in mev/mev mice: anterior prostate 0.4 times, ventral 0.19 times, dorsal 0.35 times and lateral 0.28 times those of the respective regions in wt/wt mice. Microscopically, mev/mev mice revealed scarce and large prostatic ducts, acini severely atrophic with empty lumen and scarce loose epithelial component forming tufts and infoldings, and hyperplastic changes in fibromuscular stroma.
  
  
  CONCLUSIONS
  The prostate of mev/mev mice exhibits signs of aberrant differentiation and the resulting phenotype may be related to the loss of function of SHP-1. Prostatic anomalies in these mice affect, together with defects in sperm maduration, for their sterility. These data suggest SHP-1 plays an important role in prostate epithelial morphogenesis.
authors.affiliation: Servicio de Urología, Hospital Universitario de Getafe, Departamento Clínico, Facultad de Ciencias Biomédicas, Universidad Europea de Madrid, Madrid, España. Electronic address: anagtello@yahoo.es.
authors.forename: A
authors.initials: A
authors.lastname: García-Tello
authors.affiliation: Servicio de Urología, Hospital Universitario de Getafe, Departamento Clínico, Facultad de Ciencias Biomédicas, Universidad Europea de Madrid, Madrid, España.
authors.forename: J C
authors.initials: JC
authors.lastname: Angulo
authors.affiliation: Grupo Cromatina y Enfermedad, Programa Epigenética y Biología del Cáncer, Instituto de Investigación Biomédica Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Barcelona, España; Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Alcalá de Henares, Madrid, España.
authors.forename: J
authors.initials: J
authors.lastname: Rodriguez-Ubreva
authors.affiliation: Servicio de Urología, Hospital Universitario de Getafe, Departamento Clínico, Facultad de Ciencias Biomédicas, Universidad Europea de Madrid, Madrid, España.
authors.forename: G
authors.initials: G
authors.lastname: Andrés
authors.affiliation: Servicio de Anatomía Patológica, Hospital Universitario de Cruces, Universidad del País Vasco, Baracaldo, Vizcaya, España.
authors.forename: J I
authors.initials: JI
authors.lastname: López
authors.affiliation: Servicio de Urología, Hospital Universitario Príncipe de Asturias, Universidad de Alcalá, Alcalá de Henares, Madrid, España.
authors.forename: M
authors.initials: M
authors.lastname: Sánchez-Chapado
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Alcalá de Henares, Madrid, España.
authors.forename: P
authors.initials: P
authors.lastname: López-Ruiz
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Alcalá de Henares, Madrid, España.
authors.forename: B
authors.initials: B
authors.lastname: Colás
mesh_terms: D000818:Animals; D008297:Male; D051379:Mice; D009154:Mutation; D011467:Prostate / Q000033:anatomy & histology*; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000235:genetics*
chemical_list: D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6
keywords: Anatomy; Anatomía; Diferenciación; Differentiation; Motheaten viable mouse; Prostate; Próstata; Ratón motheaten viable; SHP-1