title: Carnosine Inhibits the Proliferation of Human Gastric Carcinoma Cells by Retarding Akt/mTOR/p70S6K Signaling.
abstract: Carnosine (β-alanyl-L-histidine), described as an enigmatic peptide for its antioxidant, anti-aging and especially antiproliferation properties, has been demonstrated to play an anti-tumorigenic role in certain types of cancer. However, its function in human gastric carcinoma remains unclear. In this study, the effect of carnosine on cell proliferation and its underlying mechanisms were investigated in the cultured human gastric carcinoma cells. The mTOR signaling axis molecules were analyzed in carnosine treated cells. The results showed that treatment with carnosine led to proliferation inhibition, cell cycle arrest in the G0/G1 phase, apoptosis increase, and inhibition of mTOR signaling activation by decreasing the phosphorylation of Akt, mTOR and p70S6K, suggesting that proliferation inhibition of carnosine in human gastric carcinoma was through the inhibition of Akt/mTOR/p70S6K pathway, and carnosine would be a mimic of rapamycin.
authors.affiliation: 1. Key Laboratory of Liver Disease, Center of Infectious Diseases, Guangzhou 458 Hospital, Guangzhou, China ; 3. Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai, China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: 2. Department of Pharmacology, School of Pharmacy and Institute of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Miao
authors.affiliation: 1. Key Laboratory of Liver Disease, Center of Infectious Diseases, Guangzhou 458 Hospital, Guangzhou, China ; 4. Institute of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.affiliation: 1. Key Laboratory of Liver Disease, Center of Infectious Diseases, Guangzhou 458 Hospital, Guangzhou, China.
authors.forename: Guangze
authors.initials: G
authors.lastname: Liu
authors.affiliation: 1. Key Laboratory of Liver Disease, Center of Infectious Diseases, Guangzhou 458 Hospital, Guangzhou, China ; 4. Institute of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Peng
authors.affiliation: 2. Department of Pharmacology, School of Pharmacy and Institute of Biomedical Sciences, Fudan University, Shanghai, China ; 5. Department of pharmacology, Taishan Medical University, Tai'an, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Xin
authors.affiliation: 3. Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai, China.
authors.forename: Binghua
authors.initials: B
authors.lastname: Jiao
authors.affiliation: 1. Key Laboratory of Liver Disease, Center of Infectious Diseases, Guangzhou 458 Hospital, Guangzhou, China.
authors.forename: Xiangping
authors.initials: X
authors.lastname: Kong
keywords: Carnosine; gastric carcinoma cell; mTOR signalling pathway; proliferation; rapamycin