title: Identification of thyroid hormone receptor homologs in the fluke Opisthorchis felineus (Platyhelminthes).
abstract: The liver fluke, Opisthorchis felineus of the Opisthorchiidae family, is a well-known causative agent of opisthorchiasis in Russia and Europe. The aim of this work was to identify genes encoding thyroid hormone receptors in O. felineus, and to analyze the expression of possible target genes in response to treatment with exogenous thyroid hormones. We identified two genes encoding thyroid hormone receptors in the O. felineus genome, THRA and THRB. The genes were differentially expressed through the life cycle. The maximal level of mRNA expression of THRA1 and THRB was observed in adult worms. Treatment of the worms with triiodothyronine and thyroxine resulted in an increase in glucose 6-phosphatase mRNA expression and a decrease in malate dehydrogenase mRNA expression, potential gene targets of thyroid hormones. These data indicate that thyroid hormone receptors may perform essential roles in physiological processes in adult O. felineus.
authors.affiliation: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia. Electronic address: pmaria@yandex.ru.
authors.forename: Maria Y
authors.initials: MY
authors.lastname: Pakharukova
authors.affiliation: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
authors.forename: Nikita I
authors.initials: NI
authors.lastname: Ershov
authors.affiliation: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Vorontsova
authors.affiliation: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Shilov
authors.affiliation: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
authors.forename: Tatyana I
authors.initials: TI
authors.lastname: Merkulova
authors.affiliation: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
authors.forename: Viatcheslav A
authors.initials: VA
authors.lastname: Mordvinov
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D005952:Glucose-6-Phosphatase / Q000096:biosynthesis; D008291:Malate Dehydrogenase / Q000096:biosynthesis; D009891:Opisthorchis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D011988:Receptors, Thyroid Hormone / Q000235:genetics / Q000378:metabolism*; D013974:Thyroxine / Q000378:metabolism; D014284:Triiodothyronine / Q000378:metabolism
chemical_list: D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D008291:Malate Dehydrogenase; D005952:Glucose-6-Phosphatase; D013974:Thyroxine
keywords: Host–parasite interaction; Thyroid hormone receptor; Trematoda