title: Efficacy of Ankaferd Blood Stopper on bone healing in diabetic rats: a stereological and histopathological study.
abstract: We evaluated the effects of Ankaferd Blood Stopper (ABS) and routine antibiotic prophylaxis (AP) on early healing of bone defects in diabetic rats. We used 48 rats in the study. Diabetes was induced in 24 rats using streptozotocin; the remaining 24 healthy untreated rats served as controls. Twelve of the diabetic rats and 12 of the healthy rats were treated with AP for 3 days before surgery. Bilateral bone defects were created in the mandible of all animals. ABS was applied to the defects on the left sides of the mandibles, while nothing was applied to the right sides. Animals were sacrificed on days 7 and 14 after operation and examined for histopathology and by stereology. The volume of newly formed bone was significantly less in the diabetic rats on both days 7 and 14. Local administration of ABS significantly increased the mean volume of newly formed bone in both diabetic and nondiabetic rats at days 7 and 14. No significant difference in new bone formation was found between AP and ABS treatment in diabetic rats. Both AP and local administration of ABS have beneficial effects on bone healing in diabetic animals.
authors.affiliation: Ondokuz Mayıs University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery , Samsun , Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Bulut
authors.forename: B
authors.initials: B
authors.lastname: Baş
authors.forename: B Z
authors.initials: BZ
authors.lastname: Altunkaynak
authors.forename: B
authors.initials: B
authors.lastname: Bekçioğlu
authors.forename: G
authors.initials: G
authors.lastname: Erdem Koç
authors.forename: E
authors.initials: E
authors.lastname: Gönülol
authors.forename: M E
authors.initials: ME
authors.lastname: Önger
authors.forename: S
authors.initials: S
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001846:Bone Development / Q000187:drug effects; D001842:Bone and Bones / Q000473:pathology*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D017102:Fracture Healing / Q000187:drug effects*; D006490:Hemostatics / Q000627:therapeutic use*; D008297:Male; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000627:therapeutic use*; D028321:Plant Preparations / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000900:Anti-Bacterial Agents; D006490:Hemostatics; D010936:Plant Extracts; D028321:Plant Preparations; C539751:ankaferd blood stopper
keywords: Ankaferd Blood Stopper; antibiotic prophylaxis; bone; diabetes; histopathology; regeneration; stereology