title: Matrix metalloproteinase-9: its interplay with angiogenic factors in inflammatory bowel diseases.
abstract: Matrix metalloproteinase- (MMP-) 9 is one of the main metalloproteinases reported to be involved in extracellular matrix degradation and recently also in triggering of angiogenic switch in the course of inflammatory bowel diseases (IBD). The goal of our studies was to estimate in one experimental setting the levels of MMP-9 in sera of Crohn's Disease (CD) and ulcerative colitis (UC) patients and to evaluate its possible diagnostic potential in comparison with other biochemical markers and selected proinflammatory and angiogenic factors. The study group included 176 subjects (CD = 64, UC = 85, control = 27). Concentrations of serum MMP-9 were significantly higher in active than inactive forms of IBD, being higher in active UC than in active CD. Both in the case of CD and UC serum MMP-9 positively correlated with disease activity, IL-6 levels, platelet and leukocyte count, midkine, and PDGF-BB, as well as in UC with ESR and in CD with CRP, IL-1, and VEGF-A. Diagnostic accuracy of MMP-9 in distinguishing active UC from active CD was 66%, and displayed higher specificity than CRP (79.0% versus 61.6%, resp.). Evaluation of serum MMP-9 concentrations could aid in differentiation of active UC from active CD. MMP-9 correlated better with inflammatory and angiogenic parameters in CD than in UC.
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-358 Wroclaw, Poland.
authors.forename: Malgorzata
authors.identifier: 0000-0003-4624-0109
authors.initials: M
authors.lastname: Matusiewicz
authors.affiliation: Department of Gastroenterology and Hepatology, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland.
authors.forename: Katarzyna
authors.identifier: 0000-0003-3650-9311
authors.initials: K
authors.lastname: Neubauer
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-358 Wroclaw, Poland.
authors.forename: Magdalena
authors.identifier: 0000-0001-9640-4883
authors.initials: M
authors.lastname: Mierzchala-Pasierb
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-358 Wroclaw, Poland ; Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-358 Wroclaw, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Krzystek-Korpacka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D042501:Angiogenic Proteins / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000097:blood* / Q000175:diagnosis / Q000201:enzymology; D003424:Crohn Disease / Q000097:blood* / Q000175:diagnosis / Q000201:enzymology; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D042501:Angiogenic Proteins; D015415:Biomarkers; D002097:C-Reactive Protein; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9