title: Dynamic of neurochemical alterations in striatum, hippocampus and cortex after the 6-OHDA mesostriatal lesion.
abstract: Immediate neurochemical alterations produced by 6-OHDA could explain the general toxic pattern in the central nervous system. However, no evidences describe the effects of 6-OHDA on early changes of neurotransmitters in rats' striatum, cortex and hippocampus. In our study, unilateral 6-OHDA injection into medial forebrain bundle (MFB) was used in rats, then five neurotransmitters were analyzed at 3, 6, 12, 24, 48 and 72 h, respectively. Results showed that 6-OHDA injection caused a sharp decline of striatal dopamine (DA) levels in the first 12h followed by a further reduction between 12 and 48 h. However, striatal levels of homovanillic acid (HVA) were stable in the first 12h and showed a marked reduction between 12 and 24h. Striatal levels of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) decreased linearly for 72 h, whereas levels of norepinephrine (NE) showed a slight reduction in the first 48 h, and returned back to normal afterwards. Striatal HVA/DA ratio increased significantly in the first 12h, but 5-HIAA/5-HT ratio showed a sharp increase between 12 and 72 h. Besides, neurochemical alterations were also found in hippocampus and cortex, and the correlations of neurotransmitters were analyzed. Our study indicated that NE system had little influence in the early phase of 6-OHDA injection, moreover, early neurochemical alterations were involved with striatum, hippocampus and cortex.
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: Xue-Hong
authors.initials: XH
authors.lastname: Gui
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: Zhong-Feng
authors.initials: ZF
authors.lastname: Xue
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Huang
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: Ruo-Ming
authors.initials: RM
authors.lastname: Fang
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: Xue-Hong
authors.initials: XH
authors.lastname: Ke
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China. Electronic address: fangyq2@163.com.
authors.forename: Yong-Qi
authors.initials: YQ
authors.lastname: Fang
mesh_terms: D018663:Adrenergic Agents / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D009448:Neurochemistry; D018377:Neurotransmitter Agents / Q000378:metabolism; D016627:Oxidopamine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D018663:Adrenergic Agents; D018377:Neurotransmitter Agents; D016627:Oxidopamine
keywords: Cortex; Dynamic analysis; Hippocampus; Neurotransmitters; Parkinson's disease; Striatum