title: Mesenchymal stem cells from different murine tissues have differential capacity to metabolize extracellular nucleotides.
abstract: Mesenchymal stem cells (MSCs) have shown a great potential for cell-based therapy and many different therapeutic purposes. Despite the recent advances in the knowledge of MSCs biology, their biochemical and molecular properties are still poorly defined. Ecto-nucleoside triphosphate diphosphohydrolases (E-NTPDases) and ecto-5'-nucleotidase (eNT/CD73) are widely expressed enzymes that hydrolyze extracellular nucleotides, generating an important cellular signaling cascade. Currently, studies have evidenced the relationship between the purinergic system and the development, maintenance, and differentiation of stem cells. The objective of this study is to identify the NTPDases and eNT/CD73 and compare the levels of nucleotide hydrolysis on MSCs isolated from different murine tissues (bone marrow, lung, vena cava, kidney, pancreas, spleen, skin, and adipose tissue). MSCs from all tissues investigated expressed the ectoenzymes at different levels. In MSCs from pancreas and adipose tissue, the hydrolysis of triphosphonucleosides was significantly higher when compared to the other cells. The diphosphonucleosides were hydrolyzed at a higher rate by MSC from pancreas when compared to MSC from other tissues. The differential nucleotide hydrolysis activity and enzyme expression in these cells suggests that MSCs play different roles in regulating the purinergic system in these tissues. Overall MSCs are an attractive adult-derived cell population for therapies, however, the fact that ecto-nucleotide metabolism can affect the microenvironment, modulating important events, such as immune response, makes the assessment of this metabolism an important part of the characterization of MSCs to be applied therapeutically.
authors.affiliation: Departamento de Ciências Básicas da Saúde e Laboratório de Biologia Celular, Universidade Federal de Ciências da Saúde de Porto Alegre-UFCSPA, Porto Alegre, RS, Brazil.
authors.forename: Isabele C
authors.initials: IC
authors.lastname: Iser
authors.forename: Paula A
authors.initials: PA
authors.lastname: Bracco
authors.forename: Carlos E I
authors.initials: CE
authors.lastname: Gonçalves
authors.forename: Rafael F
authors.initials: RF
authors.lastname: Zanin
authors.forename: Nance B
authors.initials: NB
authors.lastname: Nardi
authors.forename: Guido
authors.initials: G
authors.lastname: Lenz
authors.forename: Ana Maria O
authors.initials: AM
authors.lastname: Battastini
authors.forename: Márcia R
authors.initials: MR
authors.lastname: Wink
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D059630:Mesenchymal Stem Cells / Q000201:enzymology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009711:Nucleotides / Q000378:metabolism*; D011755:Pyrophosphatases / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D009711:Nucleotides; D015720:5'-Nucleotidase; D011755:Pyrophosphatases; C021895:nucleoside triphosphate pyrophosphatase
keywords: CD73; E-NTPDASES; ECTONUCLEOTIDASES; MSC; PURINERGIC SIGNALING