title: Effect of azithromycin on enhancement of methane production from waste activated sludge.
abstract: In the methane production from waste activated sludge (WAS), complex bacterial interactions in WAS have been known as a major contribution to methane production. Therefore, the influence of bacterial community changes toward methane production from WAS was investigated by an application of antibiotics as a simple means for it. In this study, azithromycin (Azm) as an antibiotic was mainly used to observe the effect on microbial changes that influence methane production from WAS. The results showed that at the end of fermentation, Azm enhanced methane production about twofold compared to control. Azm fostered the growth of acid-producing bacterial communities, which synthesized more precursors for methane formation. DGGE result showed that the hydrolysis as well as acetogenesis stage was improved by the dominant of B1, B2 and B3 strains, which are Clostridium species. In the presence of Azm, the total population of archaeal group was increased, resulting in higher methane productivity achievement.
authors.affiliation: Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, Kitakyushu, 808-0196, Japan.
authors.forename: Minh Tuan
authors.initials: MT
authors.lastname: Nguyen
authors.forename: Toshinari
authors.initials: T
authors.lastname: Maeda
authors.forename: Mohd Zulkhairi
authors.initials: MZ
authors.lastname: Mohd Yusoff
authors.forename: Hiroaki I
authors.initials: HI
authors.lastname: Ogawa
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017963:Azithromycin / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D008697:Methane / Q000096:biosynthesis*; D050296:Microbial Viability / Q000187:drug effects; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D012722:Sewage; D017963:Azithromycin; D008697:Methane