title: Measurement of luciferase rhythms.
abstract: Firefly luciferase (LUC) is a sensitive and versatile reporter for the analysis of gene expression. Transgenic plants carrying CLOCK GENE promoter:LUC fusions can be assayed with high temporal resolution. LUC measurement is sensitive, noninvasive, and nondestructive and can be readily automated, greatly facilitating genetic studies. For these reasons, LUC fusion analysis is a mainstay in the study of plant circadian clocks.
authors.affiliation: Department of Biological Sciences, Dartmouth College, Hanover, NH, 03755, USA, C.Robertson.McClung@Dartmouth.Edu.
authors.forename: C Robertson
authors.initials: CR
authors.lastname: McClung
authors.forename: Qiguang
authors.initials: Q
authors.lastname: Xie
mesh_terms: D057906:Circadian Clocks / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified
chemical_list: D008156:Luciferases