title: Transcriptional analysis of Pseudomonas aeruginosa infected Caenorhabditis elegans.
abstract: Here, we describe a protocol for the extraction of total RNA from C. elegans infected with P. aeruginosa. The protocol excludes P. aeruginosa cells that have not been ingested by the nematodes and yields total RNA that can be used for detection of transcripts from both host and pathogen.
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, UK.
authors.forename: Ashleigh
authors.initials: A
authors.lastname: MacKenzie
authors.forename: Lewis
authors.initials: L
authors.lastname: Stewart
authors.forename: Paul A
authors.initials: PA
authors.lastname: Hoskisson
authors.forename: Nicholas P
authors.initials: NP
authors.lastname: Tucker
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000382:microbiology*; D003850:Deoxyribonuclease I / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D011552:Pseudomonas Infections / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D018510:RNA, Helminth / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D018510:RNA, Helminth; D003850:Deoxyribonuclease I