title: Usefulness of circulating microRNAs for the prediction of early preeclampsia at first-trimester of pregnancy.
abstract: To assess the usefulness of circulating microRNAs (miRNAs) as non-invasive molecular biomarkers for early prediction of preeclampsia, a differential miRNA profiling analysis was performed in first-trimester pooled sera from 31 early preeclampsia patients, requiring delivery before 34 weeks of gestation, and 44 uncomplicated pregnancies using microfluidic arrays. Among a total of 754 miRNAs analyzed, the presence of 63 miRNAs (8%) was consistently documented in the sera from preeclampsia and control samples. Nevertheless, only 15 amplified miRNAs (2%) seemed to be differentially, although modestly, represented (fold change range: 0.4-1.4). After stem loop RT-qPCR from individual samples, the statistical analysis confirmed that none of the most consistent and differentially represented miRNAs (3 overrepresented and 4 underrepresented) were differentially abundant in serum from preeclamptic pregnancies compared with serum from normal pregnancies. Therefore, maternal serum miRNA assessment at first-trimester of pregnancy does not appear to have any predictive value for early preeclampsia.
authors.affiliation: 1] Human Molecular Genetics Group, IDIBELL, L'Hospitalet de Llobregat, 08907 Barcelona, Spain [2].
authors.forename: Ana
authors.initials: A
authors.lastname: Luque
authors.affiliation: 1] Human Molecular Genetics Group, IDIBELL, L'Hospitalet de Llobregat, 08907 Barcelona, Spain [2].
authors.forename: Abduljalil
authors.initials: A
authors.lastname: Farwati
authors.affiliation: 1] Department of Maternal-Fetal Medicine, Institute Clínic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic-IDIBAPS, University of Barcelona and Centre for Biomedical Research on Rare Diseases (CIBERER), Barcelona, Spain [2] Department of Obstetrics and Gynecology, Fondazione Cà Granda, Ospedale Maggiore Policlinico; Università degli Studi di Milano, Milan, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Crovetto
authors.affiliation: Department of Maternal-Fetal Medicine, Institute Clínic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic-IDIBAPS, University of Barcelona and Centre for Biomedical Research on Rare Diseases (CIBERER), Barcelona, Spain.
authors.forename: Fatima
authors.initials: F
authors.lastname: Crispi
authors.affiliation: Department of Maternal-Fetal Medicine, Institute Clínic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic-IDIBAPS, University of Barcelona and Centre for Biomedical Research on Rare Diseases (CIBERER), Barcelona, Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Figueras
authors.affiliation: Department of Maternal-Fetal Medicine, Institute Clínic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic-IDIBAPS, University of Barcelona and Centre for Biomedical Research on Rare Diseases (CIBERER), Barcelona, Spain.
authors.forename: Eduard
authors.initials: E
authors.lastname: Gratacós
authors.affiliation: Human Molecular Genetics Group, IDIBELL, L'Hospitalet de Llobregat, 08907 Barcelona, Spain.
authors.forename: Josep M
authors.initials: JM
authors.lastname: Aran
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D011225:Pre-Eclampsia / Q000097:blood* / Q000175:diagnosis*; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000097:blood*
chemical_list: D015415:Biomarkers; D035683:MicroRNAs