title: MiR-145 inhibits osteosarcoma cells proliferation and invasion by targeting ROCK1.
abstract: MicroRNAs (miRNAs) contribute to the development and progression of various types of human cancers. The aim of this study was to study the role of miR-145 and to identify its functional target gene in osteosarcoma (OS) cells. We found that miR-145 was reduced in OS tissues and cell lines. Enforced expression of miR-145 inhibited cell proliferation, migration, and invasion abilities of MG-63 cells. Furthermore, we revealed that Rho-associated protein kinase 1 (ROCK1) was a target of miR-145 in OS. Finally, we found that silencing of ROCK1 performed similar effects with miR-145 in MG-63 cells, and ROCK1 was inversely correlated with miR-145 in OS tissues. Collectively, these data indicate that miR-145 may act as a tumor suppressor and contributes to the progression of OS through targeting ROCK1.
authors.affiliation: Department of Orthopaedic Trauma, TianJin Hospital, Jiefang Road No. 406, Hexi District, 300211, Tianjin, China, enqi_litj@163.com.
authors.forename: Enqi
authors.initials: E
authors.lastname: Li
authors.forename: Jinli
authors.initials: J
authors.lastname: Zhang
authors.forename: Tianxiang
authors.initials: T
authors.lastname: Yuan
authors.forename: Baotong
authors.initials: B
authors.lastname: Ma
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology*; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases