title: Isolation of nuc mutant isolates of Staphylococcus aureus from bovine clinical mastitis.
abstract: Isolates of Staphylococcus aureus with a mutation in the nuclease (nuc) gene were recovered from cases of bovine mastitis in Poland. Three S. aureus isolates from cows in one herd had a 42 base pair duplication in the nuc gene. These isolates belonged to sequence type 97 (ST97) and clonal complex 97 (CC97). They had a different spa type and multiple-locus variable-number tandem-repeat fingerprinting (MLVF) subtype than a S. aureus isolate without the nuc mutation from the same herd. Isolation of nuc mutant S. aureus strains from cases of bovine mastitis may confound diagnostic PCRs based on detection of the nuc gene.
authors.affiliation: Department of Microbiology, National Veterinary Research Institute, Pulawy, Poland. Electronic address: ewa.zastempowska@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Zastempowska
authors.affiliation: Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Orczykowska-Kotyna
authors.affiliation: Department of Microbiology, National Veterinary Research Institute, Pulawy, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Lassa
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D005260:Female; D008414:Mastitis, Bovine / Q000382:microbiology*; D008836:Micrococcal Nuclease / Q000235:genetics* / Q000378:metabolism; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D009154:Mutation; D011044:Poland; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013203:Staphylococcal Infections / Q000662:veterinary; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008836:Micrococcal Nuclease
keywords: Bovine mastitis; Methicillin-susceptible Staphylococcus aureus; Nuclease gene