title: Biochemical characterisation and assessment of fibril-forming ability of collagens extracted from Bester sturgeon Huso huso × Acipenser ruthenus.
abstract: Collagens purified from Bester sturgeon organs were characterised biochemically, and their fibril-forming abilities and fibril morphologies formed in vitro clarified. Yields of collagens were 2.1%, 11.9%, 0.4%, 18.1%, 0.4%, 0.8% and 0.03% (collagen dry weight/tissue wet weight) from scales, skin, muscle, swim bladder, digestive tract, notochord and snout cartilage, respectively. Using SDS-PAGE and amino acid composition analyses, collagens from scales, skin, muscle, the swim bladder and digestive tract were characterised as type I, and collagens from the notochord and snout cartilage as type II. Denaturation temperatures of the collagens, measured using circular dichroism, were 29.6, 26.8, 29.0, 32.9, 31.6 and 36.3 °C in scales, skin, muscle, swim bladder, digestive tract, and notochord, respectively. For fibril formation, swim bladder and skin collagen showed a more rapid rate of increase in turbidity, a shorter time to attain the maximum turbidity, and formed thicker fibrils compared with porcine tendon type I collagen.
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido 041-8611, Japan. Electronic address: ocean_zhangxi@yahoo.co.jp.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Ookawa
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Yongkai
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ura
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Adachi
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Takagi
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000825:Animal Structures / Q000737:chemistry; D000818:Animals; D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification; D029941:Fish Proteins / Q000737:chemistry* / Q000302:isolation & purification; D005399:Fishes / Q000235:genetics*; D006824:Hybridization, Genetic; D012867:Skin / Q000737:chemistry; D013552:Swine; D013696:Temperature
chemical_list: D000596:Amino Acids; D029941:Fish Proteins; D003094:Collagen
keywords: Denaturation temperature; Notochord; SEM; Type I collagen; Type II collagen