title: Hierarchical silicon nanospikes membrane for rapid and high-throughput mechanical cell lysis.
abstract: This letter reports an efficient and compatible silicon membrane combining the physical properties of nanospikes and microchannel arrays for mechanical cell lysis. This hierarchical silicon nanospikes membrane was created to mechanically disrupt cells for a rapid process with high throughput, and it can be assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical overetching to form ultrasharp nanospikes in situ along the edges of the microchannel arrays. The intracellular protein and nucleic acid concentrations obtained using the proposed membrane within a short period of time were quantitatively higher than those obtained by routine, conventional acoustic and chemical lysis methods.
authors.affiliation: Department of Mechanical Engineering, Berkeley Sensor & Actuator Center and ‡Department of Bioengineering, University of California , Berkeley, California 94720, United States.
authors.forename: Hongyun
authors.initials: H
authors.lastname: So
authors.forename: Kunwoo
authors.initials: K
authors.lastname: Lee
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Seo
authors.forename: Niren
authors.initials: N
authors.lastname: Murthy
authors.forename: Albert P
authors.initials: AP
authors.lastname: Pisano
mesh_terms: D002460:Cell Line; D056945:Hep G2 Cells; D006801:Humans; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D049329:Nanostructures / Q000737:chemistry*; D009696:Nucleic Acids / Q000378:metabolism; D016062:Porosity; D011506:Proteins / Q000378:metabolism; D012825:Silicon / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D009696:Nucleic Acids; D011506:Proteins; D012825:Silicon