title: Application of ultrasound on monitoring the evolution of the collagen fiber reinforced nHAC/CS composites in vivo.
abstract: To date, fiber reinforce scaffolds have been largely applied to repair hard and soft tissues. Meanwhile, monitoring the scaffolds for long periods in vivo is recognized as a crucial issue before its wide use. As a consequence, there is a growing need for noninvasive and convenient methods to analyze the implantation remolding process in situ and in real time. In this paper, diagnostic medical ultrasound was used to monitor the in vivo bone formation and degradation process of the novel mineralized collagen fiber reinforced composite which is synthesized by chitosan (CS), nanohydroxyapatite (nHA), and collagen fiber (Col). To observe the impact of cells on bone remodeling process, the scaffolds were planted into the back of the SD rats with and without rat bone mesenchymal stem cells (rBMSCs). Systematic data of scaffolds in vivo was extracted from ultrasound images. Significant consistency between the data from the ultrasound and DXA could be observed (P < 0.05). This indicated that ultrasound may serve as a feasible alternative for noninvasive monitoring the evolution of scaffolds in situ during cell growth.
authors.affiliation: Department of Ultrasonic Diagnosis, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The Second Clinical Medical College of Southern Medical University, Guangzhou 510282, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
authors.affiliation: The Second Clinical Medical College of Southern Medical University, Guangzhou 510282, China.
authors.forename: He
authors.identifier: 0000-0002-5769-4766
authors.initials: H
authors.lastname: Li
authors.affiliation: The Second Clinical Medical College of Southern Medical University, Guangzhou 510282, China.
authors.forename: Huiting
authors.initials: H
authors.lastname: Tan
authors.affiliation: The Second Clinical Medical College of Southern Medical University, Guangzhou 510282, China.
authors.forename: Huajun
authors.initials: H
authors.lastname: Li
authors.affiliation: The Second Clinical Medical College of Southern Medical University, Guangzhou 510282, China.
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedic surgery and Traumatology, Freiburg University Hospital, Freiburg, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Niemeyer
authors.affiliation: Department of Orthopaedic surgery and Traumatology, Freiburg University Hospital, Freiburg, Germany.
authors.forename: Matin
authors.initials: M
authors.lastname: Yaega
authors.affiliation: Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology; D002113:Calcification, Physiologic / Q000187:drug effects / Q000502:physiology; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008422:Materials Testing / Q000379:methods*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D053761:Nanocomposites / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014463:Ultrasonography / Q000379:methods*
chemical_list: D003094:Collagen; D048271:Chitosan; D017886:Durapatite