title: SHSST cyclodextrin complex prevents the fibrosis effect on CCl₄-induced cirrhotic cardiomyopathy in rats through TGF-β pathway inhibition effects.
abstract: Patients with liver cirrhosis also have subtle cardiac structure or function abnormalities. This cardiac dysfunction commonly occurs in 56% of waiting orthotopic liver transplantation (OLT) patients and is defined as cirrhotic cardiomyopathy (CCM). Up to now, there is no standard treatment because CCM does not have a solidly established diagnosis and is based on high clinical suspicion. The liver function of CCM is particularly limited, making patients vulnerable to more drug treatments. Here, we use silymarin (100 mg/kg/day), baicalein (30 mg/kg/day), San Huang Shel Shin Tang (SHSST, 30 mg/kg/day) and β-cyclodextrin modified SHSST (SHSSTc, 30 and 300 mg/kg/day) treatments for a CCl4-induced CCM rat model. The results show that silymarin, baicalein and SHSST treatments can only slightly reduce the collagen accumulation in CCM rat hearts. However, SHSSTc treatment protects the heart in CCM and significantly inhibits collagen acumination and the fibrosis regulating transforming growth factor-β (TGF-β) pathway expression. SHSSTc treatments further reduced the heart weight and the ratio between left ventricular weight (LVW) and tibia length (TL). This experimental data show that water solubility improved β-cyclodextrin modified Chinese herbal medicine formula (SHSSTc) can provide an excellent heart protection effect through TGF-β pathway inhibition.
authors.affiliation: Graduate Institute of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan. littleraxg@gmail.com.
authors.forename: Cheng-Hsun
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan. Wei-Jen.Ting@outlook.com.
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Ting
authors.affiliation: Department of Nursing, Mei Ho University, 23 Pingguang Road, Pingtung 91202, Taiwan. x00003023@mail.meiho.edu.tw.
authors.forename: Cecilia Hsuan
authors.initials: CH
authors.lastname: Day
authors.affiliation: Department of Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, 325, Section 2, Chenggong Road, Neihu District, Taipei 114, Taiwan. wxyz670628@yahoo.com.tw.
authors.forename: Da-Tong
authors.initials: DT
authors.lastname: Ju
authors.affiliation: Department of Pathology, Changhua Christian Hospital, 135 Nanxiao Street, Changhua 50006, Taiwan. 1867@cch.org.tw.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Yeh
authors.affiliation: Department of Hospital and Health Care Administration, China Nan University of Pharmacy & Science, 60, Section 1, Erren Road, Rende District, Tainan 71710, Taiwan. alanjack@mail.chna.edu.tw.
authors.forename: Li-Chin
authors.initials: LC
authors.lastname: Chung
authors.affiliation: School of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan. fujenntsai@yahoo.com.
authors.forename: Fu-Jenn
authors.initials: FJ
authors.lastname: Tsai
authors.affiliation: Department of Healthcare Administration, Asia University, 500 Lioufeng Road, Taichung 41354, Taiwan. changhaitsai@yahoo.com.tw.
authors.forename: Chang-Hai
authors.initials: CH
authors.lastname: Tsai
authors.affiliation: Graduate Institute of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan. yhtsai@mail.cmu.edu.tw.
authors.forename: Yuhsin
authors.initials: Y
authors.lastname: Tsai
authors.affiliation: Graduate Institute of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan. cyhuang@mail.cmu.edu.tw.
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D002251:Carbon Tetrachloride; D009202:Cardiomyopathies / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D004337:Drug Carriers / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D044950:Flavanones / Q000627:therapeutic use; D006321:Heart / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000150:complications*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D012838:Silymarin / Q000627:therapeutic use; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D000975:Antioxidants; D004337:Drug Carriers; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D012838:Silymarin; D016212:Transforming Growth Factor beta; D047392:beta-Cyclodextrins; C006680:baicalein; D002251:Carbon Tetrachloride; C031215:betadex