title: Sample treatment platform using nanoparticles to determine salinomycin in flesh and meat.
abstract: In this work, we developed a sensitive and selective analytical method of determining salinomycin in flesh and meat using a lab-built laser induced fluorescence microscope (LIFM) with nanoparticles. Two types of nanoparticles, Cy5 doped core-shell silica nanoparticles as a probe and magnetic nanoparticles (MNPs) to extract the target antibiotic from the treated sample, were synthesized and modified for chemical and enzymatic binding. After optimization, the newly developed method was applied to ham, chicken, and meat samples for the quantitative determination of salinomycin. The limits of detection (LODs) obtained from the calibration curve were 48-590 pg/mL, which was about 100-fold lower than that of the ELISA method.
authors.affiliation: Department of Chemistry, NSBI, Dankook University, 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do 448-701, Republic of Korea.
authors.forename: Jihyun
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Chemistry, NSBI, Dankook University, 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do 448-701, Republic of Korea. Electronic address: plasma@dankook.ac.kr.
authors.forename: H B
authors.initials: HB
authors.lastname: Lim
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D008856:Microscopy, Fluorescence; D053758:Nanoparticles; D011714:Pyrans / Q000032:analysis*; D012822:Silicon Dioxide; D000095662:Polyether Polyketides
chemical_list: D000900:Anti-Bacterial Agents; D011714:Pyrans; D012822:Silicon Dioxide; C010327:salinomycin; D000095662:Polyether Polyketides
keywords: Cy5-doped silica nanoparticles; Laser-induced fluorescence; Salinomycin; Sample treatment platform