title: Development of a peptide ELISA for the diagnosis of Equine arteritis virus.
abstract: A peptide-based indirect ELISA was developed to detect antibodies against Equine arteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of protein M were designed, synthesized, purified and used as antigens either alone or combined. Ninety-two serum samples obtained from the 2010 Equine viral arteritis outbreak, analyzed previously by virus neutralization, were evaluated by the ELISA here developed. The best resolution was obtained using peptide GP5. The analysis of the inter- and intraplate variability showed that the assay was robust. The results allow concluding that this peptide-based ELISA is a good alternative to the OIE-prescribed virus neutralization test because it can be standardized between laboratories, can serve as rapid screening, can improve the speed of diagnosis of EAV-negative horses and can be particularly useful for routine surveillance in large populations.
authors.affiliation: Virology, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Argentina; CONICET (CCT - La Plata), Argentina.
authors.forename: Germán Ernesto
authors.initials: GE
authors.lastname: Metz
authors.affiliation: Institute of Chemistry, University Estadual Paulista, Araraquara (UNESP), Sao Paulo, Brazil.
authors.forename: Esteban Nicolás
authors.initials: EN
authors.lastname: Lorenzón
authors.affiliation: Virology, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Argentina; CONICET (CCT - La Plata), Argentina.
authors.forename: María Soledad
authors.initials: MS
authors.lastname: Serena
authors.affiliation: Epidemiology, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Argentina.
authors.forename: Santiago Gerardo
authors.initials: SG
authors.lastname: Corva
authors.affiliation: Virology, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Argentina; CONICET (CCT - La Plata), Argentina.
authors.forename: Carlos Javier
authors.initials: CJ
authors.lastname: Panei
authors.affiliation: CONICET (CCT - La Plata), Argentina; IGEVET - CCT La Plata, Argentina.
authors.forename: Silvina
authors.initials: S
authors.lastname: Díaz
authors.affiliation: Institute of Chemistry, University Estadual Paulista, Araraquara (UNESP), Sao Paulo, Brazil.
authors.forename: Eduardo Maffud
authors.initials: EM
authors.lastname: Cilli
authors.affiliation: Virology, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Argentina; CONICET (CCT - La Plata), Argentina; IGEVET - CCT La Plata, Argentina. Electronic address: mariagabrielaecheverria@yahoo.com.ar.
authors.forename: María Gabriela
authors.initials: MG
authors.lastname: Echeverría
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology*; D018174:Arterivirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D007240:Equartevirus / Q000276:immunology* / Q000302:isolation & purification; D006734:Horse Diseases / Q000175:diagnosis* / Q000821:virology; D006736:Horses; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; C550278:GP5 protein, equine arteritis virus; D010455:Peptides; D014759:Viral Envelope Proteins
keywords: ELISA; Equine arteritis virus; Synthetic peptides