title: Pegylated glucose gold nanoparticles for improved in-vivo bio-distribution and enhanced radiotherapy on cervical cancer.
abstract: Pharmacokinetics and bio-distribution are crucial factors affecting the performance of an intravenous drug. In this study, we explore the combined use of glucose and polyethylene glycol (PEG) ligands to further improve gold nanoparticle (GNP) pharmacokinetics and bio-distribution, with the aim of using the drug for in-vivo radiotherapy. The inclusion of PEG was found to significantly prolong the half-life period, where PEG-Glu-GNPs achieved 6.17 +/- 3.71 h, compared to 1.23 +/- 0.14 h for Glu-GNPs and 1.07 +/- 0.22 h for uncoated GNPs. Our data indicates that nanoparticle size impacts cell uptake performance, with 20 nm being the optimal diameter for cancer treatment applications. Although PEG-Glu-GNPs mainly distributed in the spleen, liver, lung, and kidneys, the concentration of PEG-Glu-GNPs in tumour tissue was 20 times higher than healthy cells in the uterus and ovaries, reaching 9.22 +/- 2.41 microg/g cancer tissue at 48 h after injection. This difference in uptake holds promise for selective tumor targeting which can in turn lead to more effective radiotherapy through the interaction of X-rays and GNPs. Specifically tumor size after 47 days of treatment had reduced to (769 +/- 92) mm3 compared to (1432 +/- 269) mm3 using X-rays alone and (3514 +/- 1818) mm3 without any treatment. Moreover, the mice remained healthy without statistically significant weight loss. Results of our pharmacokinetic and bio-distribution study as well as therapeutic data for PEG-Glu-GNPs in our tumor bearing animal model demonstrate that PEG-Glu-GNPs provide excellent in-vivo stability, tumor targeting function, and radiotherapeutic enhancement effects, providing useful insights for further clinical studies.
authors.forename: Feng
authors.initials: F
authors.lastname: Geng
authors.forename: James Z
authors.initials: JZ
authors.lastname: Xing
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Ray
authors.initials: R
authors.lastname: Yang
authors.forename: Yollanda
authors.initials: Y
authors.lastname: Hao
authors.forename: Kun
authors.initials: K
authors.lastname: Song
authors.forename: Beihua
authors.initials: B
authors.lastname: Kong
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006046:Gold / Q000493:pharmacokinetics* / Q000494:pharmacology; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D011092:Polyethylene Glycols / Q000493:pharmacokinetics* / Q000494:pharmacology; D049268:Positron-Emission Tomography; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D002583:Uterine Cervical Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000532:radiotherapy* / Q000648:ultrastructure
chemical_list: D011092:Polyethylene Glycols; D006046:Gold