title: Synergistic effect of dual-functionalized fibrous scaffold with BCP and RGD containing peptide for improved osteogenic differentiation.
abstract: Over the last decade, bone tissue engineering scaffolds have been advanced owing to the bioceramic incorporation and biomimetic modification. In this report, a dual-functional fibrous scaffold with a bioceramic and biomolecule is developed, and a combined effect of a dual-modification is investigated. Biphasic calcium phosphate (BCP) is incorporated in electrospun poly (L-lactide) scaffolds, and Arg-Gly-Asp (RGD) peptide is then conjugated through the graft polymerization of acrylic acid by γ-ray irradiation. The scaffolds exhibit the intrinsic properties of BCP as well as RGD peptide, and only RGD peptide improves an adhesion and proliferation of the human mesenchymal stem cell. However, alkaline phosphatase activity and calcium formation are synergistically improved by the BCP and RGD peptide indicating that a favorable microenvironment is constructed for bone formation. Therefore, this combination strategy with bioceramic and biomolecule can be a useful tool for the bone tissue engineering.
authors.affiliation: Research Division for Industry and Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Gumgugil, Jeongeup, Jeollabuk-do 580-185, Korea.
authors.forename: Young Min
authors.initials: YM
authors.lastname: Shin
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Jo
authors.forename: Jong-Seok
authors.initials: JS
authors.lastname: Park
authors.forename: Hui-Jeong
authors.initials: HJ
authors.lastname: Gwon
authors.forename: Sung In
authors.initials: SI
authors.lastname: Jeong
authors.forename: Youn-Mook
authors.initials: YM
authors.lastname: Lim
mesh_terms: D000179:Acrylates / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002516:Ceramics / Q000627:therapeutic use; D005455:Fluorescent Antibody Technique; D005720:Gamma Rays; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000502:physiology; D009842:Oligopeptides / Q000737:chemistry; D010012:Osteogenesis / Q000502:physiology*; D058105:Polymerization / Q000187:drug effects / Q000528:radiation effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000179:Acrylates; D006882:Hydroxyapatites; D009842:Oligopeptides; C074950:hydroxyapatite-beta tricalcium phosphate; C047981:arginyl-glycyl-aspartic acid; D000469:Alkaline Phosphatase; C036658:acrylic acid
keywords: RGD peptide; biphasic calcium phosphate; bone tissue engineering; gamma-ray irradiation; surface modification