title: Comprehensive analysis of Cirsium spinosissimum Scop., a wild alpine food plant.
abstract: Plants which have been traditionally eaten by alpine populations may provide new opportunities of agricultural development for mountain regions. In this context we investigated the chemical composition of Cirsium spinosissimum (Asteraceae), a perennial thistle. Its receptacles were eaten by shepherds in Valais (Switzerland). Extracts of aerial parts were subjected to a comprehensive metabolite profiling, using a dereplication platform, combining HPLC-PDA-MS and offline microprobe NMR analysis. Twenty compounds, including various phenolic glycosides, a monoterpene lactone, a spermine derivative, and fatty acids, could be identified online, or after targeted isolation. The total phenolic content was determined, and the major flavonoids were quantitatively assessed in fresh receptacles by HPLC-PDA analysis. In addition, substances relevant for nutrition, such as β-carotene, fatty acids, ascorbic acid, and minerals, were quantified. The ethanolic extract of the receptacles showed no sign of cytotoxicity when tested in Caco-2 cells.
authors.affiliation: Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
authors.forename: Christian
authors.initials: C
authors.lastname: Abbet
authors.affiliation: Ilis Institute and Laboratory, Chemin de la Passerelle 17, CH-2503 Bienne, Switzerland.
authors.forename: Ivan
authors.initials: I
authors.lastname: Slacanin
authors.affiliation: Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Corradi
authors.affiliation: Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
authors.forename: Maria
authors.initials: M
authors.lastname: De Mieri
authors.affiliation: Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
authors.affiliation: Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland. Electronic address: olivier.potterat@unibas.ch.
authors.forename: Olivier
authors.initials: O
authors.lastname: Potterat
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis; D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid; D031180:Cirsium / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D006801:Humans; D007783:Lactones / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009753:Nutritive Value; D010636:Phenols / Q000032:analysis; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D013096:Spermine / Q000032:analysis; D013557:Switzerland; D019207:beta Carotene / Q000032:analysis
chemical_list: D005227:Fatty Acids; D005419:Flavonoids; D006027:Glycosides; D007783:Lactones; D010636:Phenols; D010936:Plant Extracts; D019207:beta Carotene; D013096:Spermine; D001205:Ascorbic Acid
keywords: Alpine food plants; Asteraceae; Cirsium spinosissimum; Linarin; Metabolite profiling; Pectolinarin