title: Helicobacter pylori detection in chronic gastritis: a comparison of staining methods.
abstract: BACKGROUND
  Helicobacter pylori is an important cause of chronic gastritis, gastric ulceration and gastric malignancies as gastric carcinoma and MALT lymphoma. Its definitive diagnosis is based on histopathology. Routine H & E stain is not very effective in its detection, immune-stains and fluorescent stains are costly. Need for simple cheap and sensitive stain has always been a topic of hot debate and extensive research.
  
  
  METHODS
  paraffin embedded blocks of all adult patients diagnosed as chronic gastritis/gastric ulceration with no accompanying gastric pathology as hypertrophic gastropathys, and neoplasias were taken into study. Three sections of 4 micron were cut and stained with routine H & E, Giemsa, and Cresyl fast violet.
  
  
  RESULTS
  Total number of patients was 50. Out of these 37 (74%) were males and 13 (26%) were females. Mean age of the patients was 50.4 years. Thirty-four percent (34%) were positive in normal H & E stain, 68% were positive in Giemsa and 76% were positive in Cresyl fast violet.
  
  
  CONCLUSIONS
  Cresyl fast violet is a good stain for diagnosis ofH. pylori gastritis.
authors.forename: Fiaz
authors.initials: F
authors.lastname: Ahmad
authors.forename: Rozina
authors.initials: R
authors.lastname: Jaffar
authors.forename: Inamullah
authors.initials: I
authors.lastname: Khan
mesh_terms: D000328:Adult; D005260:Female; D005756:Gastritis / Q000382:microbiology* / Q000473:pathology*; D016481:Helicobacter Infections / Q000473:pathology*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D013194:Staining and Labeling / Q000379:methods*; D013276:Stomach Ulcer / Q000473:pathology*