title: Influence of lactuside B on the expression of AQP4 and TRPM7 mRNAs in the cerebral cortex after cerebral ischemia injury.
abstract: OBJECTIVE
  This study aimed to determine the effect of Lactuside B isolated from Pterocypsela alata on the expression of AQP4 and TRPM7 mRNAs after cerebral ischemic injury.
  
  
  METHODS
  Brain ischemia injury was established by occluding the MCA (middle cerebral artery) for 2 h, followed by reperfusion in rats. The neurologic deficit scores were used to determine the success of the model. All drugs were intraperitoneally administered once a day (5 ml/kg). Eight animals from each group were investigated for the Na+ level, and the others were examined for AQP4 and TRPM7 mRNA changes.
  
  
  RESULTS
  Compared with the model group, the neurologic deficit scores and Na+ levels decreased in the lactuside B groups (p < 0.05 vs. p < 0.01). All lactuside B groups had significantly decreased AQP4 and TRPM7 mRNA expression compared with the model group (p < 0.05 vs. < 0.01). Dose dependence was observed between low and medium doses.
  
  
  CONCLUSIONS
  Lactuside B protected against cerebral edema and nerve cell damage caused by cerebral ischemic injury by decreasing the expression of AQP4 and TRPM7 mRNAs in the cerebral cortex of rats.
authors.affiliation: AIM: This study aimed to determine the effect of Lactuside B isolated from Pterocypsela alata on the expression of AQP4 and TRPM7 mRNAs after cerebral ischemic injury. MATERIALS AND METHODS: Brain ischemia injury was established by occluding the MCA (middle cerebral artery) for 2 h, followed by reperfusion in rats. The neurologic deficit scores were used to determine the success of the model. All drugs were intraperitoneally administered once a day (5 ml/kg). Eight animals from each group were investigated for the Na+ level, and the others were examined for AQP4 and TRPM7 mRNA changes. RESULTS: Compared with the model group, the neurologic deficit scores and Na+ levels decreased in the lactuside B groups (p < 0.05 vs. p < 0.01). All lactuside B groups had significantly decreased AQP4 and TRPM7 mRNA expression compared with the model group (p < 0.05 vs. < 0.01). Dose dependence was observed between low and medium doses. CONCLUSIONS: Lactuside B protected against cerebral edema and nerve cell damage caused by cerebral ischemic injury by decreasing the expression of AQP4 and TRPM7 mRNAs in the cerebral cortex of rats.
authors.forename: H-Q
authors.initials: HQ
authors.lastname: Zhan
authors.forename: P-F
authors.initials: PF
authors.lastname: Li
authors.forename: S-Y
authors.initials: SY
authors.lastname: Li
authors.forename: F-L
authors.initials: FL
authors.lastname: Yan
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: R-L
authors.initials: RL
authors.lastname: Liu
authors.forename: H-F
authors.initials: HF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000235:genetics*; D001929:Brain Edema / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019610:Cytoprotection; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004334:Drug Administration Schedule; D005960:Glucosides / Q000008:administration & dosage / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D007274:Injections, Intraperitoneal; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism; D050053:TRPM Cation Channels / Q000235:genetics*; D013997:Time Factors
chemical_list: C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D005960:Glucosides; D018696:Neuroprotective Agents; D012333:RNA, Messenger; D050053:TRPM Cation Channels; C581344:lactuside B; D012964:Sodium; C486584:Trpm7 protein, rat