title: PCR array analysis of gene expression profiles in chronic active Epstein-Barr virus infection.
abstract: To determine the host cellular gene expression profiles in chronic active Epstein-Barr virus infection (CAEBV), peripheral blood samples were obtained from three patients with CAEBV and investigated using a PCR array analysis that focused on T-cell/B-cell activation. We identified six genes with expression levels that were tenfold higher in CAEBV patients compared with those in healthy controls. These results were verified by quantitative reverse transcription-PCR. We identified four highly upregulated genes, i.e., IL-10, IL-2, IFNGR1, and INHBA. These genes may be involved in inflammatory responses and cell proliferation, and they may contribute to the development and progression of CAEBV.
authors.affiliation: Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
authors.forename: Masanao
authors.initials: M
authors.lastname: Murakami
authors.affiliation: Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Hashida
authors.affiliation: Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Imajoh
authors.affiliation: Department of Pediatrics, Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Maeda
authors.affiliation: Department of Laboratory Medicine, Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
authors.forename: Mikio
authors.initials: M
authors.lastname: Kamioka
authors.affiliation: Faculty of Medicine, Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Senda
authors.affiliation: Department of Pediatrics, Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sato
authors.affiliation: Department of Pediatrics, Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
authors.forename: Mikiya
authors.initials: M
authors.lastname: Fujieda
authors.affiliation: Department of Pediatrics, Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Wakiguchi
authors.affiliation: Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi 783-8505, Japan. Electronic address: daibatam@kochi-u.ac.jp.
authors.forename: Masanori
authors.initials: M
authors.lastname: Daibata
mesh_terms: D016022:Case-Control Studies; D002908:Chronic Disease; D016207:Cytokines / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020031:Epstein-Barr Virus Infections / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D016207:Cytokines
keywords: CAEBV; Cytokine; Epstein–Barr virus; Gene profile; PCR array