title: Gene transfer: transduction.
abstract: Bacteriophages able to propagate on Pseudomonas strains are very common and can be easily isolated from natural environments or lysogenic strains. The development of transducing systems has allowed bacterial geneticists to perform chromosome analyses and mutation mapping. Moreover, these systems have also been proved to be a successful tool for molecular microbiologists to introduce a foreign gene or a mutation into the chromosome of a bacterial cell. This chapter provides a description of the phage methodology illustrated by Adams in 1959 and applicable to strain PAO1 derivatives.
authors.affiliation: Department of Science, University Roma Tre, V. le Marconi 446, 00146, Rome, Italy, emanuela.frangipani@uniroma3.it.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Frangipani
mesh_terms: D001435:Bacteriophages; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000821:virology; D017186:Titrimetry; D014161:Transduction, Genetic / Q000379:methods*