title: Label-free quantitative proteomics reveals the dynamics of proteasome complexes composition and stoichiometry in a wide range of human cell lines.
abstract: The proteasome is the main proteolytic system involved in intracellular proteins homeostasis in eukaryotes. Although the structure of proteasome complexes has been well characterized, the distribution of its activators and associated proteins are less studied. Here, we determine the composition and the stoichiometry of proteasome complexes and their associated proteins in a wide range of human cell lines using a one-step affinity purification method and a label-free quantitative proteomic approach. We show that proteasome complexes are highly dynamic protein assemblies, the activity of which being regulated at different levels by variations in the stoichiometry of bound regulators, in the composition of catalytic subunits and associated proteins, and in the rate of the 20S catalytic core complex assembly.
authors.affiliation: CNRS; IPBS (Institut de Pharmacologie et de Biologie Structurale) ; 205 route de Narbonne, F-31077 Toulouse, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Fabre
authors.forename: Thomas
authors.initials: T
authors.lastname: Lambour
authors.forename: Luc
authors.initials: L
authors.lastname: Garrigues
authors.forename: Manuelle
authors.initials: M
authors.lastname: Ducoux-Petit
authors.forename: François
authors.initials: F
authors.lastname: Amalric
authors.forename: Bernard
authors.initials: B
authors.lastname: Monsarrat
authors.forename: Odile
authors.initials: O
authors.lastname: Burlet-Schiltz
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Bousquet-Dubouch
mesh_terms: D002460:Cell Line; D006706:Homeostasis; D006801:Humans; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000378:metabolism*; D060066:Protein Interaction Maps; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics
chemical_list: D018832:Molecular Chaperones; D021122:Protein Subunits; D046988:Proteasome Endopeptidase Complex