title: Interactions of tamoxifen with distearoyl phosphatidylcholine multilamellar vesicles: FTIR and DSC studies.
abstract: Interactions of a non-steroidal antiestrogen drug, tamoxifen (TAM), with distearoyl-sn-glycero-3-phosphatidylcholine (DSPC) multilamellar liposomes (MLVs) were investigated as a function of drug concentration (1-15 mol%) by using two noninvasive techniques, namely Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). FTIR spectroscopy results show that increasing TAM concentrations (except 1 mol%) increased the wavenumbers of the CH2 stretching modes, implying an disordering effect for DSPC MLVs both in the gel and liquid crystalline phases. The bandwidth values of the CH2 stretchings except for 1 mol% increased when TAM concentrations increased for DSPC liposomes, indicating an increase in the dynamics of liposomes. The CO stretching and PO2- antisymmetric double bond stretching bands were analyzed to study interactions of TAM with head groups of lipids. As the concentrations of TAM increased, dehydration occurred around these functional groups in the polar part of the lipids. The DSC studies on thermal properties of DSPC lipids indicate that TAM eliminated the pre transition, shifted the main phase transition to lower temperatures and broadened the phase transition curve of the liposomes.
authors.affiliation: Department of Physics, Faculty of Science, Ege University, 35100 İzmir, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Bilge
authors.affiliation: Department of Physics, Faculty of Science, Ege University, 35100 İzmir, Turkey.
authors.forename: Ipek
authors.initials: I
authors.lastname: Sahin
authors.affiliation: Department of Physics, Faculty of Science, Ege University, 35100 İzmir, Turkey. Electronic address: nadide.kazanci@ege.edu.tr.
authors.forename: Nadide
authors.initials: N
authors.lastname: Kazanci
authors.affiliation: Department of Biological Sciences, Middle East Technical University, 06531 Ankara, Turkey.
authors.forename: Feride
authors.initials: F
authors.lastname: Severcan
mesh_terms: D002152:Calorimetry, Differential Scanning; D045744:Cell Line, Tumor / Q000187:drug effects; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008567:Membranes, Artificial; D009369:Neoplasms / Q000473:pathology; D010713:Phosphatidylcholines / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013629:Tamoxifen / Q000737:chemistry*; D013696:Temperature
chemical_list: D008055:Lipids; D008081:Liposomes; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D013629:Tamoxifen; C010942:1,2-distearoyllecithin
keywords: DSPC; Differential scanning calorimetry; Fourier transform infrared; MLVs; Tamoxifen