title: IL-33 enhanced the proliferation and constitutive production of IL-13 and IL-5 by fibrocytes.
abstract: Interleukin-33 appears to play important roles in the induction of allergic airway inflammation. However, whether IL-33 is involved in airway remodeling remains unclear. Because fibrocytes contribute to tissue remodeling in the setting of chronic inflammation, we examined the effects of IL-33 on fibrocyte functions. Fibrocytes were generated in vitro from peripheral blood mononuclear cells by culturing in the presence of platelet derived growth factors and the cells were stimulated with IL-33. IL-33 enhanced cell proliferation, α-SMA expression, and pro-MMP-9 activity by the fibrocytes without increasing endogenous transforming growth factor-β1 production. Fibrocytes constitutively expressed IL-13 and IL-5, and their production was augmented by stimulation with IL-33. Dexamethasone inhibited the functions of fibrocytes, but IL-33 made fibrocytes slightly refractory to the inhibitory effect of dexamethasone in terms of IL-13 production. Montelukast suppressed IL-13 production by nonstimulated fibrocytes but not those stimulated by IL-33. These findings suggest that IL-33 is involved in the airway remodeling process through its modulation of fibrocyte function independent of antigen stimulation. IL-33 might partially reduce the therapeutic effects of glucocorticoid and cysteinyl leukotriene receptor antagonist on fibrocyte-mediated Th2 responses.
authors.affiliation: Department of Pediatrics, Faculty of Medical Sciences, University of Fukui, 23-3 Shimoaizuki, Matsuoka, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Hisako
authors.initials: H
authors.lastname: Hayashi
authors.affiliation: Department of Pediatrics, Faculty of Medical Sciences, University of Fukui, 23-3 Shimoaizuki, Matsuoka, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kawakita
authors.affiliation: Department of Pediatrics, Faculty of Medical Sciences, University of Fukui, 23-3 Shimoaizuki, Matsuoka, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Okazaki
authors.affiliation: Department of Pediatrics, Faculty of Medical Sciences, University of Fukui, 23-3 Shimoaizuki, Matsuoka, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Murai
authors.affiliation: Department of Pediatrics, Faculty of Medical Sciences, University of Fukui, 23-3 Shimoaizuki, Matsuoka, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Motoko
authors.initials: M
authors.lastname: Yasutomi
authors.affiliation: Department of Pediatrics, Faculty of Medical Sciences, University of Fukui, 23-3 Shimoaizuki, Matsuoka, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Yusei
authors.initials: Y
authors.lastname: Ohshima
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000199:Actins / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D003521:Cyclopropanes; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D000072179:Interleukin-1 Receptor-Like 1 Protein; D007378:Interleukins / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D011804:Quinolines / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism; D013440:Sulfides
chemical_list: D000085:Acetates; D000199:Actins; D003521:Cyclopropanes; C079241:IL1RL1 protein, human; D000072179:Interleukin-1 Receptor-Like 1 Protein; D007378:Interleukins; D011804:Quinolines; D011956:Receptors, Cell Surface; D013440:Sulfides; D003907:Dexamethasone; C093875:montelukast