title: Intrahypothalamic injection of cannabidiol increases the extracellular levels of adenosine in nucleus accumbens in rats.
abstract: Cannabidiol (CBD) is a constituent of Cannabis sativa that promotes wakefulness as well as enhances endogenous levels of wake-related neurotransmitters, including dopamine. However, at this date, the effects of CBD on the sleep-inducing molecules, such as adenosine (AD), are unknown. Here, we report that intrahypothalamic injection of CBD (10μg/1μL) increases the extracellular levels of AD collected from nucleus accumbens. Furthermore, the pharmacodynamic of this drug shows that effects on the contents of AD last 2h post-injection. These preliminary findings suggest that CBD promotes the endogenous accumulation of AD.
authors.affiliation: Laboratorio de Neurociencias Moleculares e Integrativas, Escuela de Medicina, División Ciencias de la Salud, Universidad Anáhuac Mayab, Mérida, Yucatán, Mexico.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Mijangos-Moreno
authors.affiliation: Laboratorio de Neurociencias Moleculares e Integrativas, Escuela de Medicina, División Ciencias de la Salud, Universidad Anáhuac Mayab, Mérida, Yucatán, Mexico.
authors.forename: Alwin
authors.initials: A
authors.lastname: Poot-Aké
authors.affiliation: Laboratorio de Neurobiología, Departamento de Neurociencias, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Yucatán, Mexico.
authors.forename: Gloria
authors.initials: G
authors.lastname: Arankowsky-Sandoval
authors.affiliation: Laboratorio de Neurociencias Moleculares e Integrativas, Escuela de Medicina, División Ciencias de la Salud, Universidad Anáhuac Mayab, Mérida, Yucatán, Mexico. Electronic address: eric.murillo@anahuac.mx.
authors.forename: Eric
authors.initials: E
authors.lastname: Murillo-Rodríguez
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D002185:Cannabidiol / Q000494:pharmacology*; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism*; D007031:Hypothalamus / Q000187:drug effects*; D008297:Male; D008845:Microinjections; D009714:Nucleus Accumbens / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D002185:Cannabidiol; D000241:Adenosine
keywords: Adenosine; HPLC; Hypothalamus; Microdialysis; Wakefulness