title: Application of shotgun proteomics for discovery-driven protein-protein interaction.
abstract: Affinity purification of protein complexes and identification of co-purified proteins by mass spectrometry is a powerful method to discover novel protein-protein interactions. Application of this method to the study of biological systems often requires the ability to process a large number of samples. Hence, there is great need to generate proteomic workflows compatible with large-scale studies. The major goal of this protocol is to present a fast, reliable, and scalable method to characterize protein complexes by mass spectrometry to overcome the limitations of conventional geLC-MS/MS or MudPIT protocols. This method was successfully employed for the discovery and characterization of novel protein complexes in cultured yeast, mammalian cells, and mice.
authors.affiliation: Proteomics Unit, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, 21941-909, Brazil.
authors.forename: Livia
authors.initials: L
authors.lastname: Goto-Silva
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Maliga
authors.forename: Mikolaj
authors.initials: M
authors.lastname: Slabicki
authors.forename: Jimmy Rodriguez
authors.initials: JR
authors.lastname: Murillo
authors.forename: Magno
authors.initials: M
authors.lastname: Junqueira
mesh_terms: D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D011506:Proteins