title: Influence of low-intensity pulsed ultrasound on osteogenic tissue regeneration in a periodontal injury model: X-ray image alterations assessed by micro-computed tomography.
abstract: OBJECTIVE
  This study was conducted to evaluate, with micro-computed tomography, the influence of low-intensity pulsed ultrasound on wound-healing in periodontal tissues.
  
  
  METHODS
  Periodontal disease with Class II furcation involvement was surgically produced at the bilateral mandibular premolars in 8 adult male beagle dogs. Twenty-four teeth were randomly assigned among 4 groups (G): G1, periodontal flap surgery; G2, periodontal flap surgery+low-intensity pulsed ultrasound (LIPUS); G3, guided tissue regeneration (GTR) surgery; G4, GTR surgery plus LIPUS. The affected area in the experimental group was exposed to LIPUS. At 6 and 8weeks, the X-ray images of regenerated teeth were referred to micro-CT scanning for 3-D measurement.
  
  
  RESULTS
  Bone volume (BV), bone surface (BS), and number of trabeculae (Tb) in G2 and G4 were higher than in G1 and G3 (p<0.05). BV, BS, and Tb.N of the GTR+LIPUS group were higher than in the GTR group. BV, BS, and Tb.N of the LIPUS group were higher than in the periodontal flap surgery group.
  
  
  CONCLUSIONS
  LIPUS irradiation increased the number, volume, and area of new alveolar bone trabeculae. LIPUS has the potential to promote the repair of periodontal tissue, and may work effectively if combined with GTR.
authors.affiliation: Chongqing Key Laboratory for Oral Diseases and Biomedical Science, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, China.
authors.forename: Yunji
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Chongqing Key Laboratory for Oral Diseases and Biomedical Science, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, China.
authors.forename: Zhaowu
authors.initials: Z
authors.lastname: Chai
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Chongqing Key Laboratory for Oral Diseases and Biomedical Science, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Deng
authors.affiliation: Dept. of Biomedical Engineering, Chongqing Medical University, Chongqing, China.
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Chongqing Key Laboratory for Oral Diseases and Biomedical Science, The Affiliated Hospital of Stomatology, Chongqing Medical University, No. 426, North Songshi Road, Chongqing 400015, China. Electronic address: soongjl@163.com.
authors.forename: Jinlin
authors.initials: J
authors.lastname: Song
mesh_terms: D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000628:therapy*; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D004285:Dogs; D017823:Furcation Defects / Q000000981:diagnostic imaging / Q000628:therapy*; D048091:Guided Tissue Regeneration / Q000379:methods*; D021621:Imaging, Three-Dimensional; D008297:Male; D013524:Surgical Flaps; D014464:Ultrasonic Therapy / Q000379:methods*; D014945:Wound Healing / Q000502:physiology*; D055114:X-Ray Microtomography
keywords: Low-intensity pulsed ultrasound; Micro-computed tomography; Periodontal disease