title: Increased expression of the intercellular adhesion molecule-1 (ICAM-1) on peripheral blood neutrophils in acute pancreatitis.
abstract: OBJECTIVE
  Considering the important role of neutrophils' activation in the pathogenesis of acute pancreatitis (AP), the aim of our study was to evaluate the expression of leukocytes' adhesion molecules in patients with AP.
  
  
  METHODS
  Thirty-five patients (16 women and 19 men; age 32-77 years, median 56 years) with AP were prospectively included into our study. The absolute number of leukocytes was estimated by haematologic analyser. Surface neutrophils antigens (CD) were assayed by the direct fluorescence method for whole blood, using a flow cytometer.
  
  
  RESULTS
  At the day 1, significant increase of ICAM-1 expression was found in patients with severe AP (S-AP) (7280 mm(-3) vs 2850 mm(-3) in healthy control; p<0.05). In the days 2, 3 and 5 it sharply decreased and peaked again to 4860 mm(-3) at the day 10. In patients with mild AP (M-AP), not significant elevation of ICAM-1 quickly returned to normal level. In both forms of AP, neutrophil CD62L (L-selectin) expression reached the highest level at the day 1 (8800 mm(-3) and 9020 mm(-3), respectively in M-AP and S-AP, in comparison to 3400 mm(-3) in control; p<0.05). Expression of CD69 (neutrophils' marker of early activation) significantly increased in both M-AP and S-AP.
  
  
  CONCLUSIONS
  We have found an early and significant increase of peripheral blood neutrophil CD54/ICAM-1 expression, specific for S-AP but not for M-AP. It may provide a good marker predicting severe course of pancreatitis.
authors.affiliation: Department of Gastroenterology and Internal Medicine, Medical University of Bialystok, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Dabrowski
authors.affiliation: Department of Hematological Diagnostics, Medical University of Bialystok, Poland. Electronic address: joannaosada@interia.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Osada
authors.affiliation: Department of Hematological Diagnostics, Medical University of Bialystok, Poland.
authors.forename: Milena I
authors.initials: MI
authors.lastname: Dabrowska
authors.affiliation: Department of Gastroenterology and Internal Medicine, Medical University of Bialystok, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Wereszczynska-Siemiatkowska
authors.affiliation: Department of Anesthesiology and Intensive Therapy, Medical University of Bialystok, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Siemiatkowski
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D010195:Pancreatitis / Q000097:blood / Q000175:diagnosis*; D011379:Prognosis; D011446:Prospective Studies
chemical_list: D015415:Biomarkers; C573420:ICAM1 protein, human; D018799:Intercellular Adhesion Molecule-1
keywords: Acute pancreatitis; Adhesion molecules; CD69; Neutrophils