title: Role of Rab11 in planar cell polarity and apical constriction during vertebrate neural tube closure.
abstract: Epithelial folding is a critical process underlying many morphogenetic events including vertebrate neural tube closure, however, its spatial regulation is largely unknown. Here we show that during neural tube formation Rab11-positive recycling endosomes acquire bilaterally symmetric distribution in the Xenopus neural plate, being enriched at medial apical cell junctions. This mediolateral polarization was under the control of planar cell polarity (PCP) signalling, was necessary for neural plate folding and was accompanied by the polarization of the exocyst component Sec15. Our further experiments demonstrate that similar PCP-dependent polarization of Rab11 is essential for ectopic apical constriction driven by the actin-binding protein Shroom and during embryonic wound repair. We propose that anisotropic membrane trafficking has key roles in diverse morphogenetic behaviours of individual cells and propagates in a tissue by a common mechanism that involves PCP.
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
authors.forename: Olga
authors.initials: O
authors.lastname: Ossipova
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
authors.forename: Kyeongmi
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
authors.forename: Blue B
authors.initials: BB
authors.lastname: Lake
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
authors.forename: Keiji
authors.initials: K
authors.lastname: Itoh
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
authors.forename: Andriani
authors.initials: A
authors.lastname: Ioannou
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
authors.forename: Sergei Y
authors.initials: SY
authors.lastname: Sokol
mesh_terms: D000818:Animals; D016764:Cell Polarity; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D007365:Intercellular Junctions / Q000378:metabolism*; D054258:Neural Plate / Q000378:metabolism*; D054259:Neural Tube / Q000378:metabolism*; D054261:Neurulation; D033921:Vesicular Transport Proteins / Q000378:metabolism*; D014982:Xenopus laevis; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D003598:Cytoskeletal Proteins; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins