title: [Effect of transposase on the transposition activity of piggyBac transposon transfected into Toxoplasma gondii].
abstract: To determine the transfection efficiency about PBase to piggyBac transposon in transfecting to Toxoplasma gondii, T. gondii RH strain tachyzoites were transfected with plasmid PB-Toxo-RFP which was expressed piggyBac transposon with a red fluorescent protein and Toxo-PBase plasmid which is a transposable enzyme. T. gondii tachyzoites were transfected with PB-Toxo-RFP plasmid alone as control group. The expression of red fluorescent protein was detected by flow cytometry at 24 h after transfection. The transposition efficiency in PB-Toxo-RFP+Toxo-PBase group and PB-Toxo-RFP group was 73% and 43%, respectively (P < 0.01). It suggests that the PBase transposase can improve the transfection efficiency of piggyBac transposon in T. gondii tachyzoites.
authors.forename: Xin-Shuai
authors.initials: XS
authors.lastname: Song
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.forename: Ying-Guang
authors.initials: YG
authors.lastname: Zhang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Cao
authors.forename: Quan
authors.initials: Q
authors.lastname: Liu
mesh_terms: D004251:DNA Transposable Elements; D005822:Genetic Vectors; D010957:Plasmids; D014122:Toxoplasma / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection; D019895:Transposases / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements; D019895:Transposases