title: Epinecidin-1 has immunomodulatory effects, facilitating its therapeutic use in a mouse model of Pseudomonas aeruginosa sepsis.
abstract: Antimicrobial peptides (AMPs) are garnering attention as possible alternatives to antibiotics. Here, we describe the antimicrobial properties of epinecidin-1 against a multidrug-resistant clinical isolate of P. aeruginosa (P. aeruginosa R) and a P. aeruginosa strain from ATCC (P. aeruginosa ATCC 19660) in vivo. The MICs of epinecidin-1 against P. aeruginosa R and P. aeruginosa ATCC 19660 were determined and compared with those of imipenem. Epinecidin-1 was found to be highly effective at combating peritonitis infection caused by P. aeruginosa R or P. aeruginosa ATCC 19660 in mouse models, without inducing adverse behavioral effects or liver or kidney toxicity. Taken together, our results indicate that epinecidin-1 enhances the rate of survival of mice infected with the bacterial pathogen P. aeruginosa through both antimicrobial and immunomodulatory effects.
authors.affiliation: Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung, Taiwan.
authors.forename: Chieh-Yu
authors.initials: CY
authors.lastname: Pan
authors.affiliation: Department of Biotechnology, Southern Taiwan University, Yung-Kang City, Tainan County, Taiwan.
authors.forename: Jian-Chyi
authors.initials: JC
authors.lastname: Chen
authors.affiliation: Department of Biotechnology, National Formosa University, Yunlin, Taiwan.
authors.forename: Jenn-Feng
authors.initials: JF
authors.lastname: Sheen
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan.
authors.forename: Tai-Lang
authors.initials: TL
authors.lastname: Lin
authors.affiliation: Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Jiaushi, Ilan, Taiwan zoocjy@gate.sinica.edu.tw.
authors.forename: Jyh-Yih
authors.initials: JY
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D004195:Disease Models, Animal; D024901:Drug Resistance, Multiple, Bacterial; D029941:Fish Proteins / Q000138:chemical synthesis / Q000494:pharmacology*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007155:Immunologic Factors / Q000138:chemical synthesis / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology / Q000401:mortality; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D018805:Sepsis / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology / Q000401:mortality; D016019:Survival Analysis; D023382:Toxicity Tests, Acute
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D029941:Fish Proteins; D007155:Immunologic Factors; C521392:epinecidin-1, Epinephelus coioides; D015378:Imipenem