title: Mesosilica-coated ultrafine fibers for highly efficient laccase encapsulation.
abstract: In this paper, we present a simple but efficient biomimetic method to encapsulate laccase on mesoporous silica-modified electrospun (ES) ultrafine fibers. Because of the mild immobilization conditions (room temperature, aqueous condition), the encapsulated laccase retained a high activity of 94%. Because of the protection from the silica layer, the laccase worked efficiently at 60 °C and retained a long-term activity in the presence of proteinase K. After recycling for 10 times the laccase still preserved 96% of its original reactivity. More remarkably, the immobilized laccase on fibers could completely recover its activity after thermal denature, while the free laccase permanently lost the activity. We also demonstrated that the laccase on silica-coated fibers exhibited an enhanced decolorization capability of Brilliant Blue KN-R (BBKN-R) as compared to the free laccase, showing its great potential for industrial applications.
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education of China, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. xhli@swjtu.edu.cn.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Sha
authors.initials: S
authors.lastname: He
authors.forename: Qilong
authors.initials: Q
authors.lastname: Zhao
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000327:Adsorption; D001557:Benzenesulfonates / Q000737:chemistry* / Q000302:isolation & purification; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000648:ultrastructure; D042845:Laccase / Q000737:chemistry* / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure*; D010084:Oxidation-Reduction; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D001557:Benzenesulfonates; D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D042845:Laccase; C006796:brilliant blue