title: Involvement of both PKS and NRPS in antibacterial activity in Lysobacter enzymogenes OH11.
abstract: Polyketides and nonribosomal peptides represent two large families of natural products (NPs) with diverse structures and important functions. They are synthesized by polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS), respectively. Lysobacter enzymogenes is emerging as a novel biocontrol agent against pathogens of crop plants and a new source of bioactive NPs, such as antibacterial antibiotic WAP-8294A2 and antifungal antibiotic HSAF. Genome survey of strain OH11, a Chinese L. enzymogenes isolate, detected four novel PKS, NRPS or hybrid gene clusters, designed as cluster A to D. We further individually mutated five genes (PKS or NRPS) located in these four gene clusters and showed that a PKS gene in cluster A and an NRPS gene in cluster D were involved in the antibacterial activity via a WAP-8294A2 dependent way. The data also showed that none of the five genes was associated with antifungal activity and the regulation of HSAF biosynthesis. Our results reveal the unusual regulatory role of these PKS and NRPS genes that were discovered from genome mining in L. enzymogenes.
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing, China; Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Ministry of Education, Nanjing, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: Liangcheng
authors.initials: L
authors.lastname: Du
authors.forename: Fengquan
authors.initials: F
authors.lastname: Liu
authors.forename: Feifei
authors.initials: F
authors.lastname: Xu
authors.forename: Baishi
authors.initials: B
authors.lastname: Hu
authors.forename: Vittorio
authors.initials: V
authors.lastname: Venturi
authors.forename: Guoliang
authors.initials: G
authors.lastname: Qian
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology; D047630:Depsipeptides / Q000737:chemistry / Q000494:pharmacology; D005798:Genes, Bacterial; D053528:Lysobacter / Q000737:chemistry* / Q000235:genetics; D005810:Multigene Family; D046908:Peptide Biosynthesis, Nucleic Acid-Independent; D010957:Plasmids / Q000235:genetics; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism*; D061065:Polyketides / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001688:Biological Products; D047630:Depsipeptides; D061065:Polyketides; D048630:Polyketide Synthases; C116834:lotilibcin
keywords: Lysobacter enzymogenes; WAP-8294A2; nonribosomal peptide synthetase; polyketide synthase