title: The antibacterial activity of phytochemically characterised fractions from Folium Syringae.
abstract: To identify the most active antimicrobial fraction of Folium Syringae, four common pathogens were used in an in vitro screening. The results indicated that the combination of the 30% and 60% ethanol fraction (FSC) obtained from the water extraction was the most active fraction with a minimal inhibitory concentration of 0.65 mg mL(-1). FSC was also found to be able to protect mice from a lethal infection of Staphylococcus aureus at clinical dosage (0.2 g kg(-1)) with a survival rate of 83.3%. The antibacterial activity of FSC was then tested using the serum pharmacology method which revealed that FSC exhibits a more long-lasting activity than the positive control (levofloxacin hydrochloride). The main components were confirmed to be iridoid glycosides and flavones by HPLC-MS analysis.
authors.affiliation: a Department of Pharmacognosy and Utilization Key Laboratory of Northeast Plant Materials , School of Traditional Chinese Medicine, Shenyang Pharmaceutical University , 103 Wenhua Road, Shenyang 110016 , P.R. China.
authors.forename: Zhengyuan
authors.initials: Z
authors.lastname: Zhou
authors.forename: Na
authors.initials: N
authors.lastname: Han
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Liu
authors.forename: Jinling
authors.initials: J
authors.lastname: Wang
authors.forename: Huanzhang
authors.initials: H
authors.lastname: Xia
authors.forename: Dezu
authors.initials: D
authors.lastname: Miao
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D047309:Flavones / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D057889:Iridoid Glycosides / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D044949:Kaempferols / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D047311:Luteolin / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D013211:Staphylococcus aureus / Q000187:drug effects; D031662:Syringa / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D047309:Flavones; D006027:Glycosides; D057889:Iridoid Glycosides; D044949:Kaempferols; C038066:syringopicroside; D047311:Luteolin
keywords: Folium Syringae; HPLC–MS; antibacterial activity; fraction; medicated serum