title: The comparison of methods for measuring oxidative stress in zebrafish brains.
abstract: The zebrafish is a versatile model organism with the potential to contribute to our understanding of the molecular pathological mechanisms underlying Alzheimer's disease (AD). An early characteristic of AD brain pathology is lipid peroxidation resulting from oxidative stress. However, changes in lipid peroxidation have not yet been assessed in zebrafish brains, and an earlier attempt to observe changes in F₂-isoprostane levels in the brains of zebrafish exposed to hypoxia was unsuccessful. In this article, we examine the utility of various assays of lipid peroxidation and more general assays of intracellular oxidative stress to detect the changes in oxidative stress in the brains of adult zebrafish exposed to hypoxia or explanted into a sodium azide solution for chemical mimicry of hypoxia. Levels of F₂-isoprostanes and F₄-neuroprostanes were low and variable in zebrafish brains such that statistically significant changes due to hypoxia or chemical mimicry of hypoxia could not be observed. However, measurement of lipid hydroperoxides did reveal significant changes in lipid peroxidation under these conditions, while analyses of catalase gene expression and an assay based on 2',7'-dicholorofluorescein oxidation also revealed changes in oxidative stress levels.
authors.affiliation: 1 Discipline of Genetics, School of Molecular and Biomedical Sciences, The University of Adelaide , Adelaide, Australia .
authors.forename: Seyyed Hani
authors.initials: SH
authors.lastname: Moussavi Nik
authors.forename: Kevin
authors.initials: K
authors.lastname: Croft
authors.forename: Trevor A
authors.initials: TA
authors.lastname: Mori
authors.forename: Michael
authors.initials: M
authors.lastname: Lardelli
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D028441:F2-Isoprostanes / Q000378:metabolism; D005260:Female; D005452:Fluoresceins / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015870:Gene Expression; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D058632:Neuroprostanes / Q000378:metabolism; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015027:Zebrafish / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D028441:F2-Isoprostanes; D005452:Fluoresceins; D008054:Lipid Peroxides; D058632:Neuroprostanes; D017382:Reactive Oxygen Species; D029961:Zebrafish Proteins; C037631:2',7'-dichlorofluorescein; D002374:Catalase