title: Measuring circadian oscillations of cytosolic-free calcium in Arabidopsis thaliana.
abstract: Circadian oscillations of cytosolic-free calcium can be measured in plants by observing luminescence of the bioluminescent calcium binding protein aequorin. Here we describe the use of intensified photon-counting CCD cameras to measure circadian oscillations in aequorin bioluminescence from Arabidopsis thaliana.
authors.affiliation: Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, CB2 3EA, UK.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Hearn
authors.forename: Alex A R
authors.initials: AA
authors.lastname: Webb
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D057906:Circadian Clocks / Q000502:physiology*; D003600:Cytosol / Q000378:metabolism*; D061848:Optical Imaging / Q000379:methods*
chemical_list: D002118:Calcium