title: Resveratrol reduces the proinflammatory effects and lipopolysaccharide- induced expression of HMGB1 and TLR4 in RAW264.7 cells.
abstract: BACKGROUND
  Resveratrol (Res) is a polyphenol anti-inflammatory agent. We have studied the link between the anti-inflammatory effects of Res and the high mobility group box 1(HMGB1) signaling pathway.
  
  
  METHODS
  Murine macrophage-like RAW264.7 cells (RAW264.7 cells) were either untreated (control) or treated with Res, LPS, or LPS + Res. Levels of IL-6, NO, and TNF-α were measured by ELISA and colorimetric assays. Expression of HMGB1 was detected by qRT-PCR, western blot, and immunofluorescence assays. Protein and mRNA expression levels of TLR4 were also examined.
  
  
  RESULTS
  Res significantly reduced the levels of IL-6, NO, and TNF-α in RAW264.7 cells exposed to LPS. Expression levels of HMGB1 (mRNA and protein) and of TLR4 in the LPS + Res-treated cells were lower than in cells treated with LPS alone.
  
  
  CONCLUSIONS
  Res can block the inflammatory effects induced by LPS in RAW264.7 cells. Down-regulation of HMGB expression may be one of the mechanisms of action of Res. Res may also influence TLR4 expression in the HMGB1-TLR4 signaling pathway.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital, School of Medicine, Zhejiang University, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Shuping
authors.initials: S
authors.lastname: Li
authors.forename: Qiao
authors.initials: Q
authors.lastname: Yang
authors.forename: Yu
authors.initials: Y
authors.lastname: Shi
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.forename: Yanning
authors.initials: Y
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Guangzhong
authors.initials: G
authors.lastname: Song
authors.forename: Hangdi
authors.initials: H
authors.lastname: Xu
authors.forename: Tianhong
authors.initials: T
authors.lastname: Wan
authors.forename: Jiliang
authors.initials: J
authors.lastname: He
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D024243:HMGB1 Protein / Q000235:genetics*; D007249:Inflammation / Q000188:drug therapy*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D013329:Structure-Activity Relationship; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D024243:HMGB1 Protein; C582808:HMGB1 protein, mouse; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D013267:Stilbenes; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D000077185:Resveratrol