title: Identification of novel genes involved in gastric carcinogenesis by suppression subtractive hybridization.
abstract: Gastric cancer (GC) is one of the most common and life-threatening types of malignancies. Identification of the differentially expressed genes in GC is one of the best approaches for establishing new diagnostic and therapeutic targets. Furthermore, these investigations could advance our knowledge about molecular biology and the carcinogenesis of this cancer. To screen for the overexpressed genes in gastric adenocarcinoma, we performed suppression subtractive hybridization (SSH) on gastric adenocarcinoma tissue and the corresponding normal gastric tissue, and eight genes were found to be overexpressed in the tumor compared with those of the normal tissue. The genes were ribosomal protein L18A, RNase H2 subunit B, SEC13, eukaryotic translation initiation factor 4A1, tetraspanin 8, cytochrome c oxidase subunit 2, NADH dehydrogenase subunit 4, and mitochondrially encoded ATP synthase 6. The common functions among the identified genes include involvement in protein synthesis, involvement in genomic stability maintenance, metastasis, metabolic improvement, cell signaling pathways, and chemoresistance. Our results provide new insights into the molecular biology of GC and drug discovery: each of the identified genes could be further investigated as targets for prognosis evaluation, diagnosis, treatment, evaluation of the response to new anticancer drugs, and determination of the molecular pathogenesis of GC.
authors.affiliation: Department of Pharmaceutical Biotechnology and Pharmaceutical Biotechnology Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran Pharmaceutical Sciences Research Center, Sari School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Islamic Republic of Iran.
authors.forename: N
authors.initials: N
authors.lastname: Mottaghi-Dastjerdi
authors.affiliation: Department of Pharmaceutical Biotechnology and Pharmaceutical Biotechnology Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran Pharmaceutical Sciences Research Center, Sari School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Islamic Republic of Iran.
authors.forename: M
authors.initials: M
authors.lastname: Soltany-Rezaee-Rad
authors.affiliation: Department of Pharmaceutical Biotechnology and Pharmaceutical Biotechnology Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Sepehrizadeh
authors.affiliation: Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Golestan, Islamic Republic of Iran.
authors.forename: G
authors.initials: G
authors.lastname: Roshandel
authors.affiliation: Department of General Surgery, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran.
authors.forename: F
authors.initials: F
authors.lastname: Ebrahimifard
authors.affiliation: Department of Pharmaceutical Biotechnology and Pharmaceutical Biotechnology Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran nsetayesh@tums.ac.ir.
authors.forename: N
authors.initials: N
authors.lastname: Setayesh
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D039601:Eukaryotic Initiation Factor-4A / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics; D009245:NADH Dehydrogenase / Q000235:genetics; D016914:Ribonuclease H / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics*; D066251:Subtractive Hybridization Techniques; D059470:Tetraspanins / Q000235:genetics
chemical_list: D002352:Carrier Proteins; C501005:MT-ATP6 protein, human; C098483:NADH dehydrogenase subunit 4; D012269:Ribosomal Proteins; C507456:SEC13 protein, human; C065609:TSPAN8 protein, human; D059470:Tetraspanins; C029609:ribosomal protein L18; D009245:NADH Dehydrogenase; C117108:cytochrome C oxidase subunit II; D003576:Electron Transport Complex IV; D039601:Eukaryotic Initiation Factor-4A; C091139:ribonuclease HII; D016914:Ribonuclease H; D025261:Mitochondrial Proton-Translocating ATPases
keywords: Gene expression profiling; gastric cancer; oncogene; overexpression; suppression subtractive hybridization