title: Piezo1 is as a novel trefoil factor family 1 binding protein that promotes gastric cancer cell mobility in vitro.
abstract: BACKGROUND
  Trefoil factor family 1 (TFF1) is a member of the TFF-domain peptide family involved in epithelial restitution and cell motility. Recently, we screened Piezo1 as a candidate TFF1-binding protein.
  
  
  OBJECTIVE
  We aimed to confirm Piezo1 as a novel TFF1 binding protein and to assess the role of this interaction in mediating gastric cancer cell mobility.
  
  
  METHODS
  This interaction was confirmed by co-immunoprecipitation and co-localisation of TFF1 and Piezo1 in GES-1 cells. We used stable RNA interference to knockdown Piezo1 protein expression and restored the expression of TFF1 in the gastric cancer cell lines SGC-7901 and BGC-823. Cell motility was evaluated using invasion assay and migration assay in vitro. The expression levels of the integrin subunits β1, β5, α1 as well as the expression of β-catenin and E-cadherin were detected by Western blot.
  
  
  RESULTS
  We demonstrate that TFF1, but not TFF2 or TFF3, bind to and co-localize with Piezo1 in the cytoplasm in vitro. TFF1 interacts with the C-terminal portion of the Piezo1 protein. Wound healing and trans-well assays demonstrated that the restored expression of TFF1 promoted cell mobility in gastric cancer cells, and this effect was attenuated by the knockdown of Piezo1. Western blots demonstrated the decreased expression of integrin β1 in Piezo1-knockdown cells.
  
  
  CONCLUSIONS
  Our data demonstrate that Piezo1 is a novel TFF1 binding protein that is important for TFF1-mediated cell migration and suggest that this interaction may be a therapeutic target in the invasion and metastasis of gastric cancer.
authors.affiliation: Department of Gastroenterology, Zhongshan Hospital Affiliated to Xiamen University, 201 Hubin South Road, Xiamen, 361004, Fujian Province, China.
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Yang
authors.forename: Ya-Pi
authors.initials: YP
authors.lastname: Lu
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Liu
authors.forename: Jian-Kun
authors.initials: JK
authors.lastname: Huang
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Liu
authors.forename: Chuan-Xing
authors.initials: CX
authors.lastname: Xiao
authors.forename: Amarsanaa
authors.initials: A
authors.lastname: Jazag
authors.forename: Jian-Lin
authors.initials: JL
authors.lastname: Ren
authors.forename: Bayasi
authors.initials: B
authors.lastname: Guleng
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006801:Humans; D047468:Immunoprecipitation; D007473:Ion Channels / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000503:physiopathology*; D000071163:Trefoil Factor-1; D000071164:Trefoil Factor-2; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D007473:Ion Channels; C515204:PIEZO1 protein, human; C051838:TFF1 protein, human; C000604007:TFF2 protein, human; D000071163:Trefoil Factor-1; D000071164:Trefoil Factor-2; D025521:Tumor Suppressor Proteins