title: Porous membrane ultrafiltration-A novel method for enrichment of the active compounds from micro-plasma samples.
abstract: To enrich the active compounds from plasma samples, a novel and simple method has been developed using a porous membrane envelope based on the ultrafiltration technique combining with high-performance liquid chromatography. The ultrafiltration device is a sealed porous membrane envelope prepared with a polypropylene sheet to effectively separate the active small molecules and large biomolecules, and a sample carrier is held inside the envelope to load plasma samples. The enrichment of hyperoside and isoquercitrin from rat plasma was used as an example. Significant factors of this method, such as membrane types, the desorption solvent, and the desorption time were optimized for the ultrafiltration method. Under the optimal conditions, correlation coefficients of 0.999 and 0.998 were obtained for hyperoside and isoquercitrin, respectively, with a linear range between 0.5 and 100μg/mL. The absolute extraction recoveries from 83.2% to 86.8% were achieved. The detection limits of the method for hyperoside and isoquercitrin were 0.22 and 0.20μg/mL, respectively. Compared with protein precipitation, solid-phase extraction and commercial ultrafiltration membrane methods, our proposed method demonstrates lower detection limits and lower cost for extraction. Also, it consumes less plasma samples and is found to be applicable to biological samples.
authors.affiliation: National Research Center for Chinese Minority Medicine, Minzu University of China, Beijing 100081, China.
authors.forename: Qingshan
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. Electronic address: ncyxoy@163.com.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Yin
authors.affiliation: College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Biying
authors.initials: B
authors.lastname: Sha
authors.affiliation: College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: You
mesh_terms: D000818:Animals; D030010:Apocynum / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004867:Equipment Design; D057230:Limit of Detection; D008567:Membranes, Artificial; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D011126:Polypropylenes / Q000737:chemistry; D016062:Porosity; D011794:Quercetin / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification; D051381:Rats; D052616:Solid Phase Extraction; D014462:Ultrafiltration / Q000295:instrumentation*
chemical_list: D008567:Membranes, Artificial; D010936:Plant Extracts; D011126:Polypropylenes; C016527:isoquercitrin; C021304:hyperoside; D011794:Quercetin
keywords: Hyperoside; Isoquercitrin; Micro-plasma samples; Porous membrane ultrafiltration device; Ultrafiltration