title: Acidic-pH-activatable fluorescence probes for visualizing exocytosis dynamics.
abstract: Live imaging of exocytosis dynamics is crucial for a precise spatiotemporal understanding of secretion phenomena, but current approaches have serious limitations. We designed and synthesized small-molecular fluorescent probes that were chemically optimized for sensing acidic intravesicular pH values, and established that they can be used to sensitively and reliably visualize vesicular dynamics following stimulation. This straightforward technique for the visualization of exocytosis as well as endocytosis/reacidification processes with high spatiotemporal precision is expected to be a powerful tool for investigating dynamic cellular phenomena involving changes in the pH value.
authors.affiliation: Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo (Japan).
authors.forename: Daisuke
authors.initials: D
authors.lastname: Asanuma
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Takaoka
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Namiki
authors.forename: Kenji
authors.initials: K
authors.lastname: Takikawa
authors.forename: Mako
authors.initials: M
authors.lastname: Kamiya
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hirose
mesh_terms: D005089:Exocytosis / Q000502:physiology*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration
chemical_list: D005456:Fluorescent Dyes
keywords: acidity; exocytosis; fluorescent probes; spatiotemporal dynamics; vesicular imaging