title: Conjugatable water-soluble Pt(II) and Pd(II) porphyrin complexes: novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering.
abstract: Measurement of oxygen tension in compressed collagen sheets was performed using matrix-embedded optical oxygen sensors based on platinum(II) and palladium(II) porphyrins supported on polyacrylamide nanoparticles. Bespoke, fully water-soluble, mono-functionalised Pt(II) and Pd(II) porphyrin complexes designed for conjugation under mild conditions were obtained using microwave-assisted metallation. The new sensors display a linear response (1/τ vs. O2) to varying oxygen tension over a biologically relevant range (7.0 × 10(-4) to 2.7 × 10(-1) mM) in aqueous solutions; a behaviour that is maintained following conjugation to polyacrylamide nanoparticles, and following embedding of the nanosensors in compressed collagen sheets, paving the way to innovative approaches for real-time resolution of oxygen gradients throughout 3D matrices useful for tissue regeneration.
authors.affiliation: Department of Chemistry, University of Hull, Hull, UK. R.W.Boyle@hull.ac.uk.
authors.forename: F
authors.initials: F
authors.lastname: Giuntini
authors.forename: V M
authors.initials: VM
authors.lastname: Chauhan
authors.forename: J W
authors.initials: JW
authors.lastname: Aylott
authors.forename: G A
authors.initials: GA
authors.lastname: Rosser
authors.forename: A
authors.initials: A
authors.lastname: Athanasiadis
authors.forename: A
authors.initials: A
authors.lastname: Beeby
authors.forename: A J
authors.initials: AJ
authors.lastname: MacRobert
authors.forename: R A
authors.initials: RA
authors.lastname: Brown
authors.forename: R W
authors.initials: RW
authors.lastname: Boyle
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D003094:Collagen / Q000737:chemistry; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D008872:Microwaves; D015335:Molecular Probes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010100:Oxygen / Q000032:analysis*; D010165:Palladium / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D023822:Tissue Engineering; D014867:Water / Q000737:chemistry; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D003094:Collagen; D056831:Coordination Complexes; D015335:Molecular Probes; D010100:Oxygen; D010165:Palladium; D010984:Platinum; D011166:Porphyrins; D014867:Water; D000099166:Polyacrylamides