title: Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini).
abstract: Non-human primates belonging to the Aotus genus have been shown to be excellent experimental models for evaluating drugs and vaccine candidates against malaria and other human diseases. The immune system of this animal model must be characterised to assess whether the results obtained here can be extrapolated to humans. Class I and II major histocompatibility complex (MHC) proteins are amongst the most important molecules involved in response to pathogens; in spite of this, the techniques available for genotyping these molecules are usually expensive and/or time-consuming. Previous studies have reported MHC-DRB class II gene typing by microsatellite in Old World primates and humans, showing that such technique provides a fast, reliable and effective alternative to the commonly used ones. Based on this information, a microsatellite present in MHC-DRB intron 2 and its evolutionary patterns were identified in two Aotus species (A. vociferans and A. nancymaae), as well as its potential for genotyping class II MHC-DRB in these primates.
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Cundinamarca, Colombia; School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, Cundinamarca, Colombia; MSc Microbiology Programme, Instituto de Biotecnología (IBUN), Universidad Nacional de Colombia, Bogotá, Cundinamarca, Colombia.
authors.forename: Carolina
authors.initials: C
authors.lastname: López
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Cundinamarca, Colombia; School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, Cundinamarca, Colombia.
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Suárez
authors.affiliation: Genetics Institute, Universidad Nacional de Colombia, Bogotá, Cundinamarca, Colombia.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Cadavid
authors.affiliation: School of Medicine, Universidad Nacional de Colombia, Bogotá, Cundinamarca, Colombia.
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Patarroyo
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Cundinamarca, Colombia; School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, Cundinamarca, Colombia.
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Patarroyo
mesh_terms: D000818:Animals; D016645:Aotidae / Q000235:genetics*; D060005:Genotyping Techniques; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D007438:Introns / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*
chemical_list: D000949:Histocompatibility Antigens Class II