title: LSCHL4 from Japonica Cultivar, which is allelic to NAL1, increases yield of indica super rice 93-11.
abstract: The basic premise of high yield in rice is to improve leaf photosynthetic efficiency and coordinate the source-sink relationship in rice plants. Quantitative trait loci (QTLs) related to morphological traits and chlorophyll content of rice leaves were detected at the stages of heading to maturity, and a major QTL (qLSCHL4) related to flag leaf shape and chlorophyll content was detected at both stages in recombinant inbred lines constructed using the indica rice cultivar 93-11 and the japonica rice cultivar Nipponbare. Map-based cloning and expression analysis showed that LSCHL4 is allelic to NAL1, a gene previously reported in narrow leaf mutant of rice. Overexpression lines transformed with vector carrying LSCHL4 from Nipponbare and a near-isogenic line of 93-11 (NIL-9311) had significantly increased leaf chlorophyll content, enlarged flag leaf size, and improved panicle type. The average yield of NIL-9311 was 18.70% higher than that of 93-11. These results indicate that LSCHL4 had a pleiotropic function. Exploring and pyramiding more high-yield alleles resembling LSCHL4 for super rice breeding provides an effective way to achieve new breakthroughs in raising rice yield and generate new ideas for solving the problem of global food safety.
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Guang-Heng
authors.initials: GH
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Shu-Yu
authors.initials: SY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Da-Li
authors.initials: DL
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Yu-Chun
authors.initials: YC
authors.lastname: Rao
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: You-Lin
authors.initials: YL
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Yao-Long
authors.initials: YL
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: De-Yong
authors.initials: DY
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Xing-Ming
authors.initials: XM
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Mei-Xian
authors.initials: MX
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China.
authors.forename: Long-Biao
authors.initials: LB
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Chuan-You
authors.initials: CY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China. Electronic address: qianqian188@hotmail.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
mesh_terms: D000483:Alleles; D002734:Chlorophyll / Q000378:metabolism; D002874:Chromosome Mapping; D017343:Genes, Plant / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D040641:Quantitative Trait Loci; D012987:Soil; D013045:Species Specificity; D014170:Transformation, Genetic
chemical_list: D012987:Soil; D002734:Chlorophyll
keywords: QTL; panicle type; pleiotropism; qLSCHL4; rice breeding; yield potential.