title: Enhancement of the nonamyloidogenic pathway by exogenous NGF in an Alzheimer transgenic mouse model.
abstract: Nerve growth factor (NGF) is an important nerve cell growth regulatory factor and has an indispensable role in the development, survival and regeneration of the cholinergic basal forebrain (CBF) neurons, and it has multiple targets when used for Alzheimer's Disease (AD) therapy. In this study, we observed whether NGF can affect cholinergic neurons to change amyloid-β precursor protein (APP) metabolism process and reduce amyloidosis in AD brains. NGF was administered intranasally to APP/PS1 double-transgenic mice for 14weeks. We observed an increase in APP695 and ADAM10 and a decrease in BACE1 and PS1 protein levels and, subsequently, a reduction in Aβ1-40 and Aβ1-42 levels and Aβ burden were present in NGF-treated mice brains, suggesting that NGF enhanced the APP nonamyloidogenic cleavage pathway and reduced the Aβ generation in the APP/PS1 transgenic mice brains.
authors.affiliation: Department of Neurobiology, China Medical University, Shenyang, PR China; Department of Anatomy, Liaoning University of Traditional Chinese Medicine, Shenyang, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Acupuncture and Massage College, Liaoning University of Traditional Chinese Medicine, Shenyang, PR China.
authors.forename: Yuli
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Anatomy, Daqing Branch of Haerbin Medical University, Daqing, PR China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Ni
authors.affiliation: Major Scientific Research Platform, Liaoning University of Traditional Chinese Medicine, Shenyang, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Neurobiology, China Medical University, Shenyang, PR China.
authors.forename: Baohui
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Neurobiology, China Medical University, Shenyang, PR China. Electronic address: xbfang@mail.cmu.edu.cn.
authors.forename: Xiubin
authors.initials: X
authors.lastname: Fang
mesh_terms: D000281:Administration, Intranasal; D000544:Alzheimer Disease / Q000235:genetics / Q000503:physiopathology*; D058227:Amyloidogenic Proteins / Q000378:metabolism*; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D020932:Nerve Growth Factor / Q000008:administration & dosage / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D058227:Amyloidogenic Proteins; D020932:Nerve Growth Factor
keywords: APP/PS1 transgenic mice; Alzheimer’s Disease; Amyloid-β precursor protein; Nerve growth factor