title: Apricot attenuates oxidative stress and modulates of Bax, Bcl-2, caspases, NFκ-B, AP-1, CREB expression of rats bearing DMBA-induced liver damage and treated with a combination of radiotherapy.
abstract: We evaluated the ability of apricot to attenuate apoptosis and oxidative stress developed during the process of 7,12-dimethylbenz[a]anthracene (DMBA) and radiotherapy in the liver of rats bearing liver damage. Fifty female Wistar rats were divided into 7 groups; (i) normal control rats; (ii) rats fed with standard diet with apricot (20%), (ii) rats fed with standard diet and administrated 6 gray radiotherapy with Co 60 device applied to a single fraction, (iv) rats fed with standard diet and administered intraperitoneally DMBA (20mg/kg), (v) rats fed with standard diet and administered DMBA and 6 gray radiotherapy, (vi) rats fed with standard rat diet and administered DMBA and supplemented apricot, (vii) rats fed with standard diet supplemented apricot administered DMBA and radiotherapy (RT) for 6weeks. Expression of Bax, caspase 3, and glutathione activity decreased in the liver but liver expression of NF-κB, AP-1, CREB, Bcl-2 and ALT, AST, 5'NT, MDA, NO levels increased in DMBA-induced liver damage rats. In conclusion, the results suggest that apricot supplementation and irradiation given in combination, offer maximum protection against DMBA-induced hepatic carcinogenesis.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Inonu University, 44280 Malatya, Turkey. Electronic address: aysunbay.karabulut@inonu.edu.tr.
authors.forename: Aysun Bay
authors.initials: AB
authors.lastname: Karabulut
authors.affiliation: Department of Pathology, Faculty of Medicine, Inonu University, 44280 Malatya, Turkey.
authors.forename: Nese
authors.initials: N
authors.lastname: Karadag
authors.affiliation: Department of Radiation Oncology, Faculty of Medicine, Inonu University, 44280 Malatya, Turkey.
authors.forename: Simay
authors.initials: S
authors.lastname: Gurocak
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Inonu University, 44280 Malatya, Turkey.
authors.forename: Tugba
authors.initials: T
authors.lastname: Kiran
authors.affiliation: Division of Biology, Faculty of Science, Firat University, 23119 Elazig, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Tuzcu
authors.affiliation: Department of Nutrition, Faculty of Fisheries, Inonu University, 44280 Malatya, Turkey; Department of Animal Nutrition, Faculty of Veterinary, Firat University, 23119 Elazig, Turkey.
authors.forename: Kazım
authors.initials: K
authors.lastname: Sahin
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage / Q000009:adverse effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000532:radiotherapy / Q000628:therapy*; D005260:Female; D008099:Liver / Q000378:metabolism / Q000528:radiation effects; D008113:Liver Neoplasms / Q000139:chemically induced / Q000188:drug therapy; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D027861:Prunus / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D018808:Transcription Factor AP-1; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D051028:bcl-2-Associated X Protein; D015127:9,10-Dimethyl-1,2-benzanthracene; D050882:CREB-Binding Protein; C494365:Crebbp protein, rat; C505410:Casp3 protein, rat; D053148:Caspase 3
keywords: 7,12-Dimethylbenz[a]anthracene; Apoptosis; Apricot; Liver damage; Oxidative stress; Radiotherapy