title: [Micro-droplet characterization and its application for amino acid detection in droplet microfluidic system].
abstract: Recently, the droplet microfluidic system attracts interests due to its high throughput and low cost to detect and screen. The picoliter micro-droplets from droplet microfluidics are uniform with respect to the size and shape, and could be used as monodispensed micro-reactors for encapsulation and detection of single cell or its metabolites. Therefore, it is indispensable to characterize micro-droplet and its application from droplet microfluidic system. We first constructed the custom-designed droplet microfluidic system for generating micro-droplets, and then used the micro-droplets to encapsulate important amino acids such as glutamic acid, phenylalanine, tryptophan or tyrosine to test the droplets' properties, including the stability, diffusivity and bio-compatibility for investigating its application for amino acid detection and sorting. The custom-designed droplet microfluidic system could generate the uniformed micro-droplets with a controllable size between 20 to 50 microm. The micro-droplets could be stable for more than 20 h without cross-contamination or fusion each other. The throughput of detection and sorting of the system is about 600 micro-droplets per minute. This study provides a high-throughput platform for the analysis and screening of amino acid-producing microorganisms.
authors.forename: Huiling
authors.initials: H
authors.lastname: Yuan
authors.forename: Libing
authors.initials: L
authors.lastname: Dong
authors.forename: Ran
authors.initials: R
authors.lastname: Tu
authors.forename: Wenbin
authors.initials: W
authors.lastname: Du
authors.forename: Shiru
authors.initials: S
authors.lastname: Ji
authors.forename: Qinhong
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification*; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics / Q000295:instrumentation*
chemical_list: D000596:Amino Acids