title: Gabapentin, a synthetic analogue of gamma aminobutyric acid, reverses systemic acute inflammation and oxidative stress in mice.
abstract: The aim of this study was to investigate the potential anti-inflammatory and anti-oxidant effects of gabapentin (GBP) in mice. The anti-inflammatory and anti-oxidant effects were evaluated using various mediators that induce paw edema, peritonitis model, myeloperoxidase (MPO) activity, proinflammatory cytokine levels, glutathione (GSH) consumption, and malondialdehyde (MDA) production in mice. Pretreatment of mice with GBP (1 mg/kg) significantly reduced carrageenan or dextran-induced paw edema (P<0.05) when compared to vehicle group. Adding to this, GBP (1 mg/kg) significantly inhibited paw edema induced by histamine, serotonin, bradikinin, 48/80 compound, and prostaglandin E2. In the carrageenan-induced peritonitis model, GBP significantly decreased total and differential leukocyte counts and reduced the levels of MPO activity in the plantar tissue and IL-1β and TNF-α concentrations in the peritoneal exudate. The same dose of GBP also decreased the MDA concentration and increased the levels of GSH into the peritoneal fluid. In summary, our results demonstrated that GBP exhibited anti-inflammatory activity in mice by reducing the action of inflammatory mediators, neutrophil migration and proinflammatory cytokine levels, and anti-oxidant properties by decreasing the concentration of MDA and increasing the GSH content. These observations raise the possibility that GBP could be used to improve tissue resistance to damage during inflammatory conditions.
authors.affiliation: LAFFEX-Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Parnaiba-PI, 64049-550, Brazil.
authors.forename: Jordana Maia
authors.initials: JM
authors.lastname: Dias
authors.forename: Tarcisio Vieira
authors.initials: TV
authors.lastname: de Brito
authors.forename: Diva
authors.initials: D
authors.lastname: de Aguiar Magalhães
authors.forename: Pammela Weryka
authors.initials: PW
authors.lastname: da Silva Santos
authors.forename: Jalles Arruda
authors.initials: JA
authors.lastname: Batista
authors.forename: Eulina Gabriela
authors.initials: EG
authors.lastname: do Nascimento Dias
authors.forename: Heliana
authors.initials: H
authors.lastname: de Barros Fernandes
authors.forename: Samara Rodrigues Bonfim
authors.initials: SR
authors.lastname: Damasceno
authors.forename: Renan O
authors.initials: RO
authors.lastname: Silva
authors.forename: Karoline S
authors.initials: KS
authors.lastname: Aragão
authors.forename: Marcellus H L P
authors.initials: MH
authors.lastname: Souza
authors.forename: Jand-Venes R
authors.initials: JV
authors.lastname: Medeiros
authors.forename: André Luiz R
authors.initials: AL
authors.lastname: Barbosa
mesh_terms: D000208:Acute Disease; D000588:Amines / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology / Q000627:therapeutic use*; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D000077206:Gabapentin; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D011897:Random Allocation; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000588:Amines; D000893:Anti-Inflammatory Agents; D003509:Cyclohexanecarboxylic Acids; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin