title: Ratio of intracellular precursors concentration and their flux influences hyaluronic acid molecular weight in Streptococcus zooepidemicus and recombinant Lactococcus lactis.
abstract: HA molecular weight variation in Streptococcus zooepidemicus and two recombinant Lactococcus lactis strains were investigated by chemostat experiments and metabolic flux analysis (MFA). The study showed that intracellular flux ratio of UDP-GlcUA to UDP-GlcNAc correlated directly with HA molecular weight, for all the three strains. The ratio of intracellular concentration of these HA precursors also exhibited a similar trend. Phosphoglucoisomerase activity and glucose flux towards lactic acid formation were found to be the major bottlenecks for HA production in all the three strains. The study suggests that environmental conditions and genetic manipulations that balance the intracellular flux and HA precursors concentrations will result in increased molecular weight.
authors.affiliation: Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600 036, India.
authors.forename: Sneh Sanjay
authors.initials: SS
authors.lastname: Badle
authors.affiliation: Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600 036, India.
authors.forename: Guhan
authors.initials: G
authors.lastname: Jayaraman
authors.affiliation: Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600 036, India. Electronic address: kbram@iitm.ac.in.
authors.forename: K B
authors.initials: KB
authors.lastname: Ramachandran
mesh_terms: D000388:Air; D003470:Culture Media; D006820:Hyaluronic Acid / Q000737:chemistry*; D013294:Lactococcus lactis / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D018502:Streptococcus equi / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003470:Culture Media; D006820:Hyaluronic Acid
keywords: Hyaluronic acid; Lactococcus lactis; MFA; Molecular weight; Streptococcus zooepidemicus