title: DNA barcode ITS2 coupled with high resolution melting (HRM) analysis for taxonomic identification of Sideritis species growing in Greece.
abstract: Identification of genotypes in Sideritis is complicated owing to the morphological similarity and common occurrence of natural hybridisation within Sideritis species. Species- and genotype-specific DNA markers are very useful for plant identification, breeding and preservation programs. Herein, a real-time polymerase chain reaction (PCR) of ITS2 barcode region coupled with high resolution melting-curve (HRM) analysis was evaluated for an accurate, rapid and sensitive tool for species identification focusing on seven Sideritis species growing in Greece. The HRM assay developed in this study is a rapid and straightforward method for the identification and discrimination of the investigated Sideritis species. This assay is simple compared to other genotyping methods as it does not require DNA sequencing or post-PCR processing. Therefore, this method offers a new alternative for rapid detection of Sideritis species.
authors.affiliation: Cotton and Industrial Plants Institute, Hellenic Agricultural Organization "Demeter", Thermi, 57001, Thessaloniki, Greece.
authors.forename: Apostolos
authors.initials: A
authors.lastname: Kalivas
authors.forename: Ioannis
authors.initials: I
authors.lastname: Ganopoulos
authors.forename: Aliki
authors.initials: A
authors.lastname: Xanthopoulou
authors.forename: Paschalina
authors.initials: P
authors.lastname: Chatzopoulou
authors.forename: Athanasios
authors.initials: A
authors.lastname: Tsaftaris
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Madesis
mesh_terms: D001483:Base Sequence; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005819:Genetic Markers; D060005:Genotyping Techniques / Q000379:methods*; D006115:Greece; D008969:Molecular Sequence Data; D010802:Phylogeny; D058974:Phylogeography; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D031365:Sideritis / Q000145:classification* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers