title: [Clinical investigation to compare aCGH and FISH in preimplantationgenetic diagnosis of chromosome translocation carriers].
abstract: OBJECTIVE
  To investigate the clinical use of array comparative genomic hybridization (aCGH) with fluorescence in situ hybridization (FISH) in preimplantion genetic diagnosis (PGD) for reciprocal and Robertsonian translocation carriers.
  
  
  METHODS
  From Jan. 2012 to Jun. 2013, a total of 220 PGD cycles from 151 reciprocal translocation and 62 Robertsonian translocation carrier couples, including 33 cycles for reciprocal translocation carriers and 22 cycles for Robertsonian translocation carriers performed using array CGH, and 119 cycles for reciprocal translocation carriers and 46 cycles for Robertsonian translocation carriers performed using FISH were retrospectively studied. The rate of accurate diagnosis was compared between two methods.
  
  
  RESULTS
  Normal and/or balance rates of the two translocated chromosomes detected by aCGH for both reciprocal and Robertsonian translocation carriers were 38.20% (123/322) and 67.20% (127/189), significantly higher than 15.39% (195/1 267) and 30.75% (202/657) by FISH (all P < 0.05). Abnormal rates of the two translocated chromosomes detected by aCGH for both reciprocal and Robertsonian translocation carriers were 59.32% (191/322) and 30.69% (58/189), significantly lower than 83.03% (1 052/1 267) and 67.43% (443/657) by FISH (all P < 0.05). And the rate of aneu ploidy in non-translocated chromosome from reciprocal translocation embryos was 20.19% (65/322), which was significantly lower than 38.62% (13/189) from Robertsonian translocation embryos (P < 0.01).
  
  
  CONCLUSIONS
  Normal and/or balance rates of the two translocated chromosomes detected by array CGH were significantly higher than FISH. And the rate of aneuploidy in non-translocated chromosomes from reciprocal translocation embryos was significantly lower than that from Robertsonian translocation embryos.
authors.affiliation: Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Benyu
authors.initials: B
authors.lastname: Miao
authors.affiliation: Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China. Email: zhoucanquan@hotmail.com.
authors.forename: Canquan
authors.initials: C
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000782:Aneuploidy; D001706:Biopsy; D001755:Blastocyst; D025063:Chromosome Disorders / Q000175:diagnosis*; D055028:Comparative Genomic Hybridization; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis / Q000379:methods*; D014178:Translocation, Genetic