title: Low intraprostatic DHT promotes the infiltration of CD8+ T cells in BPH tissues via modulation of CCL5 secretion.
abstract: Clinical studies suggested thatandrogen might be associated with infiltrating T cells in prostate of benign prostatic hyperplasia (BPH) patients, but detail of T-cell subset and mechanism still remained unclear. The present study tested the hypothesis that intraprostatic 5 α -dihydrotestosterone (DHT) exerts effects on T cells recruitment by BPH epithelial cells. Prostate tissues from 64 cases of BPH patients after transurethral resection of prostate (TURP) were divided into 2 groups: (1) no medication history; (2) administration of 5 α -reductase type II inhibitor-finasteride 5 mg daily for at least 6 months before surgery. Group 2 presented significantly higher CD8+ T cells infiltration than group 1, but no changes in CD4+ T cells (immunohistochemistry and flow cytometry). In vitro study more CD8+ T cell migrated to the prostate tissue lysates from group 2 and BPH-1 cells in low DHT condition. Transcription of chemokine (C-C motif) Ligand 5 (CCL5) mRNA in BPH-1 cells and chemokine (C-C motif) receptor 5 (CCR5) mRNA in CD8+ T cells were upregulated in low DHT condition (q-PCR). CCL5 expression was also identified to be higher in group 2 prostate tissues by IHC. This study suggested that intraprostatic DHT may participate in regulating inflammatory response which was induced by human prostatic epithelial cell, via modulating CCL5 secretion.
authors.affiliation: Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, Beijing 100034, China ; National Research Center for Genitourinary Oncology, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, Beijing 100034, China ; National Research Center for Genitourinary Oncology, Beijing, China.
authors.forename: Shuai
authors.identifier: 0000-0001-5507-1808
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, Beijing 100034, China ; National Research Center for Genitourinary Oncology, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Surgery, China-Japanese Friendship Hospital, Beijing, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, Beijing 100034, China ; National Research Center for Genitourinary Oncology, Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, Beijing 100034, China ; National Research Center for Genitourinary Oncology, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.affiliation: Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, Beijing 100034, China ; National Research Center for Genitourinary Oncology, Beijing, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, Beijing 100034, China ; National Research Center for Genitourinary Oncology, Beijing, China.
authors.forename: Mengkui
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, Beijing 100034, China ; National Research Center for Genitourinary Oncology, Beijing, China.
authors.forename: Tianjing
authors.initials: T
authors.lastname: Lv
authors.affiliation: Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, Beijing 100034, China ; National Research Center for Genitourinary Oncology, Beijing, China.
authors.forename: Qun
authors.identifier: 0000-0002-2110-9721
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, Beijing 100034, China ; National Research Center for Genitourinary Oncology, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D018946:Chemokine CCL5; D013196:Dihydrotestosterone / Q000378:metabolism*; D018120:Finasteride / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism
chemical_list: C483024:CCL5 protein, human; D018946:Chemokine CCL5; D013196:Dihydrotestosterone; D018120:Finasteride