title: Development and application of a monoclonal antibody against grouper iridovirus (GIV) major capsid protein.
abstract: The major capsid protein (MCP) is a main structural protein of iridoviruses, and is used as a marker for the identification, differentiation and classification of ranaviruses. In the present study, six monoclonal antibodies (mAbs) against recombinant MCP of grouper iridovirus (GIV) were produced and characterized. All of the six mAbs were of IgG1 isotype. Among the mAbs, GIV-MCP-mAb-21 showed the highest reactivity in ELISA and was used to further characterize the expression of GIV-MCP during viral replication. RT-PCR and Western blot analyses revealed that GIV-MCP is a late gene during GIV infection. By immunofluorescence assay, the presence of GIV-MCP was observed in not only the cytoplasm but also the nucleus of GIV-infected cells, a surprising finding that might indicate additional role of GIV-MCP. In conclusion, the newly established GIV-MCP-mAbs are a valuable tool for GIV diagnostic and future studies on GIV pathogenesis.
authors.affiliation: Department of Horticulture, National Ilan University, Yilan, Taiwan; Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan.
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Lin
authors.affiliation: Department of Nursing, Chang Gung University of Science and Technology, Taoyuan, Taiwan; Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
authors.forename: Chian-Jiun
authors.initials: CJ
authors.lastname: Liou
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan.
authors.forename: Yeong-Hsiang
authors.initials: YH
authors.lastname: Cheng
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan.
authors.forename: Hui-Chen
authors.initials: HC
authors.lastname: Hsu
authors.affiliation: Department of Horticulture, National Ilan University, Yilan, Taiwan.
authors.forename: Jinn-Chin
authors.initials: JC
authors.lastname: Yiu
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Pinwen Peter
authors.initials: PP
authors.lastname: Chiou
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan. Electronic address: yslai@niu.edu.tw.
authors.forename: Yu-Shen
authors.initials: YS
authors.lastname: Lai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D001483:Base Sequence; D036022:Capsid Proteins / Q000276:immunology*; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000175:diagnosis* / Q000821:virology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006825:Hybridomas; D007074:Immunoglobulin G / Q000276:immunology; D017994:Iridovirus / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D010473:Perciformes; D017995:Ranavirus / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C081132:major capsid protein, Iridovirus
keywords: Grouper; Iridovirus; Major capsid protein; Monoclonal antibody