title: Association of viral replication capacity with the pathogenicity of enterovirus 71.
abstract: Enterovirus 71 (EV71) is a major cause of hand-foot-and-mouth disease, which is associated with fatal neurological disease. The mechanism of EV71 pathogenesis remains obscure. We compared the replication capacity of the severe and mild enterovirus 71 isolates. The replication kinetics of EV71 in RD cells and ICR mice was determined by qRT-PCR. The lung, muscular, brain, intestine tissues were used for histopathological and immunohistochemical assays. The growth curves of EV71 strains in RD cells showed that the severe EV71 strains (SDLY107 and SDLY52) replicated faster and generated more viral RNA than the mild EV71 strains (SDLY11 and SDLY1). The mice infected by the severe EV71 strains (SDLY107) showed more severe clinical symptoms, pathological changes and higher viral load than the mice infected by the mild EV71 strains (SDLY11). These results suggest that there was a difference in replication capacity between the severe and mild EV71 strains, which was possibly associated with EV71 pathogenesis.
authors.affiliation: Department of Virology, School of Public Health, Shandong University, Jinan, Shandong 250012, People's Republic of China.
authors.forename: Le-le
authors.initials: LL
authors.lastname: Sun
authors.affiliation: Laiwu City Center for Disease Control and Prevention, Shandong Province 271100, People's Republic of China.
authors.forename: Jia-Kun
authors.initials: JK
authors.lastname: Wang
authors.affiliation: Shanghai City Center for Disease Control and Prevention, Shanghai 200336, People's Republic of China.
authors.forename: Xiao-qing
authors.initials: XQ
authors.lastname: Cui
authors.affiliation: Shandong Medical Equipment Quality Supervision and Inspection Center, Jinan, Shandong 250101, People's Republic of China.
authors.forename: Shu-Bin
authors.initials: SB
authors.lastname: Hao
authors.affiliation: Department of Virology, School of Public Health, Shandong University, Jinan, Shandong 250012, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Virology, School of Public Health, Shandong University, Jinan, Shandong 250012, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Shandong Medical Equipment Quality Supervision and Inspection Center, Jinan, Shandong 250101, People's Republic of China.
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Yuan
authors.affiliation: Department of Virology, School of Public Health, Shandong University, Jinan, Shandong 250012, People's Republic of China. Electronic address: wenhongling@sdu.edu.cn.
authors.forename: Hong-ling
authors.initials: HL
authors.lastname: Wen
authors.affiliation: Department of Virology, School of Public Health, Shandong University, Jinan, Shandong 250012, People's Republic of China.
authors.forename: Xue-jie
authors.initials: XJ
authors.lastname: Yu
authors.affiliation: Department of Virology, School of Public Health, Shandong University, Jinan, Shandong 250012, People's Republic of China.
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Wang
mesh_terms: D000825:Animal Structures / Q000821:virology; D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D004769:Enterovirus Infections / Q000473:pathology / Q000821:virology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008813:Mice, Inbred ICR; D014774:Virulence; D014779:Virus Replication
keywords: EV71; Pathogenicity; Replication