title: Physiopathological effects of Escherichia coli O157:H7 inoculation in weaned calves fed with colostrum containing antibodies to EspB and Intimin.
abstract: Escherichia coli O157:H7 is responsible for severe intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. Cattle are the primary reservoir for E. coli O157:H7 and the main source of infection for humans. In this study, we evaluated the ability of transferred maternal colostral antibodies against γ-Intimin C280 and EspB, to protect young weaned calves from E. coli O157:H7 infection. Hyperimmune colostra were obtained by immunization of pregnant cows with a mix of the mentioned antigens. All vaccinated cows mounted a significant IgG response against γ-Intimin C280, and EspB in sera and colostra. Colostrum-fed calves also exhibited high serum IgG titers against γ-Intimin C280 and EspB along with a rise in mucosal γ-Intimin C280-specific IgG antibodies at recto-anal junction and ileum. Additionally, 70 day-old calves received a challenge with E. coli O157:H7 but no reduction in total bacterial shedding or frequency of E. coli O157:H7 excretion from these calves was observed. Most tissue samples showed granulocyte focal infiltrations of the lamina propria and enterocyte erosion. In conclusion, up to the 70th day, the passively acquired γ-Intimin-C280 and EspB-IgG antibodies present in sera and recto-anal mucosa reached a titer insufficient to reduce EHEC O157 shedding and damages of experimentally inoculated young calves.
authors.affiliation: Instituto de Patobiología, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.
authors.forename: B C
authors.initials: BC
authors.lastname: Rabinovitz
authors.affiliation: Instituto de Patobiología, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.
authors.forename: D A
authors.initials: DA
authors.lastname: Vilte
authors.affiliation: Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Larzábal
authors.affiliation: Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Abdala
authors.affiliation: Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.
authors.forename: R
authors.initials: R
authors.lastname: Galarza
authors.affiliation: Laboratorio de Fisiopatogenia, Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: E
authors.initials: E
authors.lastname: Zotta
authors.affiliation: Laboratorio de Fisiopatogenia, Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Ibarra
authors.affiliation: Instituto de Patobiología, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.
authors.forename: E C
authors.initials: EC
authors.lastname: Mercado
authors.affiliation: Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina. Electronic address: cataldi.angeladrian@inta.gob.ar.
authors.forename: A
authors.initials: A
authors.lastname: Cataldi
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D057138:Bacterial Shedding; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D003126:Colostrum / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D019453:Escherichia coli O157; D029968:Escherichia coli Proteins / Q000276:immunology*; D022361:Escherichia coli Vaccines / Q000008:administration & dosage; D005243:Feces / Q000382:microbiology; D005260:Female; D007112:Immunity, Maternally-Acquired; D018928:Immunity, Mucosal; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D011247:Pregnancy
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C087863:EaeB protein, E coli; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; D007074:Immunoglobulin G; C073819:eaeA protein, E coli
keywords: Calves; Colostrum; EHEC O157:H7; IgG; Vaccine