title: Effect of raw Radix Rehmanniae on the pharmacokinetics of pioglitazone in rats.
abstract: Raw Radix Rehmanniae (RRR) is a frequently used traditional Chinese medicine in the treatment of diabetes mellitus according to the statistics on all of the anti-diabetic formulas recorded in New National Traditional Chinese Medicine. Pioglitazone and RRR may be co-administrated for presumably enhanced therapeutic effects because of the common indications. Therefore, the aim of the study was to evaluate the effect of RRR on the pharmacokinetics of pioglitazone in healthy rats and type 2 diabetic rats. The pharmacokinetic effect of RRR on pioglitazone was studied in healthy rats and type 2 diabetic rats. A validated UPLC-MS/MS method was used to analyze the concentration of pioglitazone in blood samples. The pharmacokinetic parameters were calculated using non-compartmental analyses by Winnonlin 5.0.1. In healthy group, the pre-treatment of RRR significantly (P<0.05) reduced the C(max) but enhanced the V/F of pioglitazone; whereas in T2DM group, signiﬁcant increase of C(max) and decrease of V/F and T½ were found after the rats were pre-treated with RRR. However, AUC(0-t) and CL/F remained unchanged in both healthy group and T2DM group. In conclusion, co-administration with RRR could alter the pharmacokinetic profiles of pioglitazone to statistically significant levels.
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China / Department of Pharmacy, Puyang Medical College, Puyang, Henan, China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Shi
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Gao
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China.
authors.forename: Yuemei
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Zhu
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China.
authors.forename: Meicun
authors.initials: M
authors.lastname: Yao
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy; D041743:Herb-Drug Interactions; D007004:Hypoglycemic Agents / Q000493:pharmacokinetics*; D008297:Male; D008516:Medicine, Chinese Traditional; D000077205:Pioglitazone; D051381:Rats; D017208:Rats, Wistar; D032267:Rehmannia; D045162:Thiazolidinediones / Q000493:pharmacokinetics*
chemical_list: D007004:Hypoglycemic Agents; D045162:Thiazolidinediones; D000077205:Pioglitazone