title: Employing TMT quantification in shotgun-MS proteomic analysis: a focus on skeletal muscle.
abstract: Quantify proteins present in a sample is the ultimate goal of a proteomic experiment. Although it might reveal countless physiological and /or pathological process on a studied model, it is still a daunting task to perform. Of the protein present in a sample, only a small number can be identified and even a lesser number quantified, each with its own weaknesses and strengths. Presented here are the "tandem mass tags" isobaric labels (TMT) and a protocol for its utilization in the quantification of skeletal muscle, which might be adapted also to other animal tissues.
authors.affiliation: Catarinense Federal Institute, Videira Campus, Rodovia SC 135, Km 125, Bairro Campo Experimental, Videira - Santa Catarina, Brazil, bruno.olv@gmail.com.
authors.forename: Bruno Menezes
authors.initials: BM
authors.lastname: de Oliveira
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D007553:Isotope Labeling; D051379:Mice; D009124:Muscle Proteins / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry*; D040901:Proteomics; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009124:Muscle Proteins