title: Development of morin-conjugated Au nanoparticles: exploring the interaction efficiency with BSA using spectroscopic methods.
abstract: In order to enhance its interaction efficiency with biomacromolecules for the usage as a therapeutic agent, we have conjugated morin, an antioxidant activity and anti-tumor drug, with citrate-coated Au nanoparticles (M-C-AuNPs). M-C-AuNPs were prepared by reducing chloroauric acid using trisodium citrate in the boiling condition, and the resulted M-C-AuNPs were characterized by UV-vis absorption spectroscopy, Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and FTIR analysis. In this article, UV-vis absorption spectroscopy in combination with fluorescence spectroscopy, and circular dichroism (CD) spectroscopy were employed to investigate the interactions between M-C-AuNPs and bovine serum albumin (BSA), C-AuNPs and BSA in a phosphate buffer at pH 7.4. By comparing the quenching constant KSV, effective quenching constant Ka, binding constant Kb and the number of binding sites n, it is clearly suggested that M-C-AuNPs could enhance the binding force of morin with BSA, which would pave the way for the design of nanotherapeutic agents with improved functionality.
authors.affiliation: Hubei Collaborative Innovation Center for Rare Metal Chemistry, Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi 435002, PR China.
authors.forename: Hua-Li
authors.initials: HL
authors.lastname: Yue
authors.affiliation: Hubei Collaborative Innovation Center for Rare Metal Chemistry, Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi 435002, PR China. Electronic address: yjhu@263.net.
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: Hubei Collaborative Innovation Center for Rare Metal Chemistry, Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi 435002, PR China.
authors.forename: Hong-Gui
authors.initials: HG
authors.lastname: Huang
authors.affiliation: Hubei Collaborative Innovation Center for Rare Metal Chemistry, Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi 435002, PR China.
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Hubei Collaborative Innovation Center for Rare Metal Chemistry, Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi 435002, PR China.
authors.forename: Bao
authors.initials: B
authors.lastname: Tu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry; D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry; D002417:Cattle; D002942:Circular Dichroism; D005419:Flavonoids / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D047309:Flavones
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D001672:Biocompatible Materials; D005419:Flavonoids; D006046:Gold; D012710:Serum Albumin, Bovine; C008548:morin; D047309:Flavones
keywords: Bovine serum albumin; Citrate-coated Au nanoparticles; Interaction efficiency; Morin-conjugated Au nanoparticles; Spectroscopy; Therapeutic agent