title: Pseudotyping of vesicular stomatitis virus with the envelope glycoproteins of highly pathogenic avian influenza viruses.
abstract: Pseudotype viruses are useful for studying the envelope proteins of harmful viruses. This work describes the pseudotyping of vesicular stomatitis virus (VSV) with the envelope glycoproteins of highly pathogenic avian influenza viruses. VSV lacking the homotypic glycoprotein (G) gene (VSVΔG) was used to express haemagglutinin (HA), neuraminidase (NA) or the combination of both. Propagation-competent pseudotype viruses were only obtained when HA and NA were expressed from the same vector genome. Pseudotype viruses containing HA from different H5 clades were neutralized specifically by immune sera directed against the corresponding clade. Fast and sensitive reading of test results was achieved by vector-mediated expression of GFP. Pseudotype viruses expressing a mutant VSV matrix protein showed restricted spread in IFN-competent cells. This pseudotype system will facilitate the detection of neutralizing antibodies against virulent influenza viruses, circumventing the need for high-level biosafety containment.
authors.affiliation: Institut für Virologie und Immunologie (IVI), Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland.
authors.forename: Gert
authors.initials: G
authors.lastname: Zimmer
authors.affiliation: Institut für Virologie und Immunologie (IVI), Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland.
authors.forename: Samira
authors.initials: S
authors.lastname: Locher
authors.affiliation: Institut für Virologie und Immunologie (IVI), Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland.
authors.forename: Marianne
authors.initials: M
authors.lastname: Berger Rentsch
authors.affiliation: Institut für Virologie und Immunologie (IVI), Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland.
authors.forename: Stefan J
authors.initials: SJ
authors.lastname: Halbherr
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D001717:Birds; D061505:Cell Surface Display Techniques / Q000379:methods*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000821:virology*; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests; D018116:Vesiculovirus / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014764:Viral Proteins; C109963:hemagglutinin, avian influenza A virus; C487630:NA protein, influenza A virus; D009439:Neuraminidase