title: The RNA-binding protein PCBP2 facilitates gastric carcinoma growth by targeting miR-34a.
abstract: Gastric carcinoma is the fourth most common cancer worldwide, with a high rate of death and low 5-year survival rate. However, the mechanism underling gastric cancer is still not fully understood. Here in the present study, we identify the RNA-binding protein PCBP2 as an oncogenic protein in human gastric carcinoma. Our results show that PCBP2 is up-regulated in human gastric cancer tissues compared to adjacent normal tissues, and that high level of PCBP2 predicts poor overall and disease-free survival. Knockdown of PCBP2 in gastric cancer cells inhibits cell proliferation and colony formation in vitro, whereas opposing results are obtained when PCBP2 is overexpressed. Our in vivo subcutaneous xenograft results also show that PCBP2 can critically regulate gastric cancer cell growth. In addition, we find that PCBP2-depletion induces apoptosis in gastric cancer cells via up-regulating expression of pro-apoptotic proteins and down-regulating anti-apoptotic proteins. Mechanically, we identify that miR-34a as a target of PCBP2, and that miR-34a is critically essential for the function of PCBP2. In summary, PCBP2 promotes gastric carcinoma development by regulating the level of miR-34a.
authors.affiliation: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Cheng-En
authors.initials: CE
authors.lastname: Hu
authors.affiliation: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Yong-Chao
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Shanghai Children's Medical Center, Shanghai, China.
authors.forename: Hui-Dong
authors.initials: HD
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China. Electronic address: huanggjfdu@sina.com.
authors.forename: Guang-Jian
authors.initials: GJ
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D055785:Gene Knockdown Techniques; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D014410:Tumor Stem Cell Assay
chemical_list: C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; C501903:PCBP2 protein, human; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016601:RNA-Binding Proteins
keywords: Apoptosis; Gastric cancer; PCBP2; miR-34a