title: Long-term viremia and fecal shedding in pups after modified-live canine parvovirus vaccination.
abstract: Canine parvovirus (CPV) modified live virus vaccines are able to infect vaccinated dogs replicating in the bloodstream and enteric mucosa. However, the exact duration and extent of CPV vaccine-induced viremia and fecal shedding are not known. With the aim to fill this gap, 26 dogs were administered two commercial vaccines containing a CPV-2 or CPV-2b strain and monitored for 28 days after vaccination. By using real-time PCR, vaccine-induced viremia and shedding were found to be long lasting for both vaccinal strains. Vaccinal CPV-2b shedding was detected for a shorter period than CPV-2 (12 against 19 mean days) but with greater viral loads, whereas viremia occurred for a longer period (22 against 19 mean days) and with higher titers for CPV-2b. Seroconversion appeared as early as 7 and 14 days post-vaccination for CPV-2b and CPV-2 vaccines, respectively. With no vaccine there was any diagnostic interference using in-clinic or hemagglutination test, since positive results were obtained only by fecal real-time PCR testing. The present study adds new insights into the CPV vaccine persistence in the organism and possible interference with diagnostic tests.
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy. Electronic address: nicola.decaro@uniba.it.
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Crescenzo
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Costantina
authors.initials: C
authors.lastname: Desario
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cavalli
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Losurdo
authors.affiliation: Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Foggia, Italy; Ospedale Veterinario Pingry, Bari, Italy.
authors.forename: Maria Loredana
authors.initials: ML
authors.lastname: Colaianni
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Gianpiero
authors.initials: G
authors.lastname: Ventrella
authors.affiliation: Ospedale Veterinario Pingry, Bari, Italy; Cocker House - Allevamento "dei Machich", Mola di Bari, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Rizzi
authors.affiliation: Ospedale Veterinario Pingry, Bari, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Aulicino
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Maria Stella
authors.initials: MS
authors.lastname: Lucente
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004279:DNA, Viral / Q000302:isolation & purification; D004283:Dog Diseases / Q000517:prevention & control*; D004285:Dogs; D005243:Feces / Q000821:virology; D005260:Female; D006385:Hemagglutination Inhibition Tests; D008297:Male; D010322:Parvoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D017993:Parvovirus, Canine; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000008:administration & dosage; D019562:Viral Load; D014765:Viral Vaccines / Q000008:administration & dosage*; D014766:Viremia / Q000175:diagnosis*; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
keywords: Canine parvovirus; Vaccination; Viremia; Virus shedding