title: Effects of intermittent training on anaerobic performance and MCT transporters in athletes.
abstract: This study examined the effects of intermittent hypoxic training (IHT) on skeletal muscle monocarboxylate lactate transporter (MCT) expression and anaerobic performance in trained athletes. Cyclists were assigned to two interventions, either normoxic (N; n = 8; 150 mmHg PIO2) or hypoxic (H; n = 10; ∼3000 m, 100 mmHg PIO2) over a three week training (5×1 h-1h30 x week(-1)) period. Prior to and after training, an incremental exercise test to exhaustion (EXT) was performed in normoxia together with a 2 min time trial (TT). Biopsy samples from the vastus lateralis were analyzed for MCT1 and MCT4 using immuno-blotting techniques. The peak power output (PPO) increased (p<0.05) after training (7.2% and 6.6% for N and H, respectively), but VO2max showed no significant change. The average power output in the TT improved significantly (7.3% and 6.4% for N and H, respectively). No differences were found in MCT1 and MCT4 protein content, before and after the training in either the N or H group. These results indicate there are no additional benefits of IHT when compared to similar normoxic training. Hence, the addition of the hypoxic stimulus on anaerobic performance or MCT expression after a three-week training period is ineffective.
authors.affiliation: ISSUL Institute of Sport Sciences University of Lausanne, Lausanne, Switzerland; Department of Physiology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Grégoire
authors.initials: G
authors.lastname: Millet
authors.affiliation: Faculty of Health Science, University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Bentley
authors.affiliation: ORION, Clinical Services Ltd, London, England.
authors.forename: Belle
authors.initials: B
authors.lastname: Roels
authors.affiliation: Department of Sport and Physical Activity, Edge Hill University, Ormskirk, Lancashire, England.
authors.forename: Lars R
authors.initials: LR
authors.lastname: Mc Naughton
authors.affiliation: Laboratoire de physiologie des Interactions EA 701, Institut de Biologie, Montpellier, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Mercier
authors.affiliation: School of Nutrition and Exercise Sciences, Deakin University, Melbourne, Victoria, Australia; Liggins Institute, University of Auckland, Auckland, New Zealand.
authors.forename: David
authors.initials: D
authors.lastname: Cameron-Smith
mesh_terms: D000328:Adult; D015308:Anaerobic Threshold / Q000502:physiology*; D056352:Athletes; D015444:Exercise / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000096:biosynthesis*; D009124:Muscle Proteins / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000378:metabolism*; D010809:Physical Fitness / Q000502:physiology*; D027981:Symporters / Q000096:biosynthesis*; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D027501:Monocarboxylic Acid Transporters; D009124:Muscle Proteins; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1; C406962:SLC16A4 protein, human