title: Necrotic regions are absent in fiber-shaped cell aggregates, approximately 100 μm in diameter.
abstract: Microscopic, fiber-shaped cell aggregates, have been used as building blocks for fabricating macroscopic three-dimensional tissue architectures, in the field of tissue engineering. In this study, we examined the occurrence of necrotic regions in the most widely used, fiber-shaped cell aggregates, approximately 100 μm in diameter. Alginate hydrogel hollow microfibers were used as templates for the cell aggregates. We demonstrated negligible necrotic region formation occurred in the cell aggregates formed in the hollow microfibers. Furthermore, we improved on previously-reported methods for preparing the hollow microfibers to avoid common microfiber tangling during the fiber preparation process.
authors.affiliation: a Department of Chemical Engineering , Graduate School of Science and Engineering, Kagoshima University , Korimoto, Kagoshima , Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Takei
authors.affiliation: a Department of Chemical Engineering , Graduate School of Science and Engineering, Kagoshima University , Korimoto, Kagoshima , Japan.
authors.forename: Jyunpei
authors.initials: J
authors.lastname: Kitazono
authors.affiliation: b Department of Gastroenterology , Nanpuh Hospital , Kagoshima , Japan.
authors.forename: Sadao
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: c Department of Pathology , Nanpuh Hospital , Kagoshima , Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Nishimata
authors.affiliation: a Department of Chemical Engineering , Graduate School of Science and Engineering, Kagoshima University , Korimoto, Kagoshima , Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoshida
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002449:Cell Aggregation; D020723:Glucuronic Acid / Q000737:chemistry; D056945:Hep G2 Cells; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D009336:Necrosis / Q000517:prevention & control; D023822:Tissue Engineering
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid
keywords: hydrogel; microfiber; necrosis; regenerative medicine; tissue engineering