title: Crystallization and preliminary X-ray crystallographic analysis of a novel α-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405.
abstract: The truncated carbohydrate-active enzyme belonging to family 43 glycoside hydrolase from Clostridium thermocellum (CtGH43) is an α-L-arabinofuranosidase that in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans. The recombinant enzyme CtGH43 from C. thermocellum was overexpressed in Escherichia coli and purified by immobilized metal-ion affinity chromatography. The recombinant CtGH43 has a molecular mass of 35.86 kDa. Preliminary structural characterization was carried out on CtGH43 crystallized from different conditions, which gave either cube-shaped or brick-shaped crystals. These diffracted to a resolution of 1.65 Å for the cubic form and 1.1 Å for the monoclinic form. Molecular replacement was used to solve the CtGH43 structure.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781 039, India.
authors.forename: Arun
authors.initials: A
authors.lastname: Goyal
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781 039, India.
authors.forename: Shadab
authors.initials: S
authors.lastname: Ahmed
authors.affiliation: CIISA-Faculdade de Medicina Veterinaria, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal.
authors.forename: Carlos M G A
authors.initials: CM
authors.lastname: Fontes
authors.affiliation: CIISA-Faculdade de Medicina Veterinaria, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal.
authors.forename: Shabir
authors.initials: S
authors.lastname: Najmudin
mesh_terms: D000595:Amino Acid Sequence; D048013:Acetivibrio thermocellus / Q000201:enzymology* / Q000235:genetics*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data
chemical_list: D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase
keywords: C. thermocellum; CtGH43; glycoside hydrolase; α-l-arabinofuranosidase