title: Expression profiles of genes in wild-type DJ-1 and A39S DJ-1 mutant cells.
abstract: OBJECTIVE
  To elucidate the role of A39S mutation of DJ-1 in the onset of Parkinson's disease (PD) and identify genes for which expressions are abnormally regulated by A39S DJ-1 mutation.
  
  
  METHODS
  We established HEK293 cell lines which stably expressed empty vector, wild-type DJ-1 and A39S mutated DJ-1 respectively. DNA microarrays were used to identify genes for which expressions change in wild-type DJ-1 cells and A39S DJ-1 mutant cells.
  
  
  RESULTS
  Compared with the cell line expression empty vector, we identified 42 differentially regulated genes (including 14 up-regulated genes and 28 down-regulated genes) in the wildtype DJ-1 cells and 8 differentially regulated genes (including 6 up-regulated genes and 2 downregulated genes) in the A39S DJ-1 mutant cells. Compared with the wild-type DJ-1 cells, only the expression of UGT2B7 gene was down-regulated in A39S DJ-1 mutant cells. These differentially regulated genes were mainly related to signal transduction, regulation of transcription, apoptosis and metabolism.
  
  
  CONCLUSIONS
  A39S mutated DJ-1 may disturb the transcriptional activities of DJ-l and involve in the pathogenesis of PD.
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Liu
authors.forename: Beisha
authors.initials: B
authors.lastname: Tang
authors.forename: Dan
authors.initials: D
authors.lastname: He
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Xiao
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Yan
authors.forename: Jifeng
authors.initials: J
authors.lastname: Guo
mesh_terms: D017209:Apoptosis; D015536:Down-Regulation; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010300:Parkinson Disease / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D015533:Transcriptional Activation; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D047908:Intracellular Signaling Peptides and Proteins