title: Self-assembly of protein fibrils into suprafibrillar aggregates: bridging the nano- and mesoscale.
abstract: We report on in vitro self-assembly of nanometer-sized α-synuclein amyloid fibrils into well-defined micrometer-sized suprafibrillar aggregates with sheet-like or cylindrical morphology depending on the ionic strength of the solution. The cylindrical suprafibrillar structures are heavily hydrated, suggesting swollen gel-like particles. In contrast to higher order structures formed by other negatively charged biopolymers, multivalent ions are not required for the suprafibrillar aggregates to form. Their formation is induced by both mono- and divalent counterions. The self-assembly process is not mediated by protein-specific interactions but rather by the cooperative action of long-range electrostatic repulsion and short-range attraction. Understanding the mechanism driving the self-assembly might give us valuable insight into the pathological formation of fibrillar superstructures such as Lewy bodies and neurites-distinct signatures of Parkinson's disease-and will open the possibility to utilize the self-assembly process for the design of novel fibril-based smart nanostructured materials.
authors.affiliation: Nanobiophysics, MESA+ Institute for Nanotechnology, Faculty of Science and Technology, University of Twente , PO Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: Slav A
authors.initials: SA
authors.lastname: Semerdzhiev
authors.forename: Dirk R
authors.initials: DR
authors.lastname: Dekker
authors.forename: Vinod
authors.initials: V
authors.lastname: Subramaniam
authors.forename: Mireille M A E
authors.initials: MM
authors.lastname: Claessens
mesh_terms: D000682:Amyloid / Q000737:chemistry; D001665:Binding Sites; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions; D016631:Lewy Bodies / Q000737:chemistry; D018625:Microscopy, Atomic Force; D009154:Mutation; D036103:Nanotechnology / Q000379:methods*; D009994:Osmolar Concentration; D010300:Parkinson Disease / Q000473:pathology*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D055672:Static Electricity; D013696:Temperature; D051844:alpha-Synuclein / Q000737:chemistry*
chemical_list: D000682:Amyloid; D007477:Ions; D011506:Proteins; D012996:Solutions; D051844:alpha-Synuclein