title: Differentially expressed genes in the cuticle and hemolymph of the silkworm, Bombyx mori, injected with the fungus Beauveria bassiana.
abstract: The most important pathogenic fungus of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), is Beauveria bassiana (Balsamo-Crivelli ) Vuillemin (Hypocreales: Clavicipitaceae), which causes significant damage to sericulture production. Therefore, diagnosing fungal disease and developing new control measures are crucial to silk production. To better understand the responsive and interactive mechanisms between the host silkworm and this fungus, variations in silkworm gene expression were investigated using the suppression subtractive hybridization method following the injection of B. bassiana conidia. Two cDNA libraries were constructed, and 140 cDNA clones were isolated. Of the 50 differentially expressed genes identified, 45 (112 clones) were identified in the forward library, and 5 (28 clones) were identified in the reverse library. Expression profiling of six of these genes by quantitative polymerase chain reaction (qPCR) verified that they were induced by the fungal challenge. The present study provides insight into the interaction between lepidopteran insects and pathogenic fungi.
authors.affiliation: 1 Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu, China.
authors.forename: Cheng-Xiang
authors.initials: CX
authors.lastname: Hou
authors.forename: Guang-Xing
authors.initials: GX
authors.lastname: Qin
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Xing-Lin
authors.initials: XL
authors.lastname: Mei
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Zhong-Yuan
authors.initials: ZY
authors.lastname: Shen
authors.forename: Xi-Jie
authors.initials: XJ
authors.lastname: Guo
mesh_terms: D000818:Animals; D052982:Beauveria / Q000502:physiology*; D061046:Biological Control Agents; D012831:Bombyx / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D005786:Gene Expression Regulation; D006458:Hemolymph / Q000378:metabolism / Q000382:microbiology; D007113:Immunity, Innate; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D034582:Integumentary System / Q000382:microbiology; D007814:Larva / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction; D013172:Spores, Fungal / Q000502:physiology; D066251:Subtractive Hybridization Techniques
chemical_list: D061046:Biological Control Agents; D019476:Insect Proteins