title: Temperature switch PCR (TSP): a gel-based molecular marker technique for investigating single nucleotide polymorphisms.
abstract: Temperature Switch PCR (TSP) is a robust single-marker single nucleotide polymorphism (SNP) genotyping technique with broad applications in genetic studies of various organisms. The technique consists of a biphasic PCR with two sets of primers, a locus-specific set and a nested locus-specific set. The PCR products can be easily assessed for polymorphism based on different band sizes using agarose gel electrophoresis.
authors.affiliation: Australian Centre for Plant Functional Genomics, The University of Adelaide, PMB 1, Glen Osmond, SA, Australia, lpthanh@ctu.edu.vn.
authors.forename: Le Phuoc
authors.initials: le P
authors.lastname: Thanh
authors.forename: Kelvin
authors.initials: K
authors.lastname: Khoo
mesh_terms: D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D017343:Genes, Plant; D005819:Genetic Markers; D060005:Genotyping Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D005819:Genetic Markers