title: Eosinophils in hereditary and inflammatory myopathies.
abstract: It is not known whether eosinophilic myositis is a specific histopathological feature of limb girdle muscular dystrophy 2A (LGMD2A). Number and location of eosinophils in skeletal muscle biopsies (n=100) was analysed by Giemsa and modified hematoxylin/eosin staining in patients with genetically confirmed myopathies (LGMD2A, LGMD2B, LGMD2L, facioscapulohumeral muscular dystrophy, dystrophinopathy), histologically confirmed idiopathic inflammatory myopathies (sporadic inclusion body myositis (sIBM), dermatomyositis (DM), polymyositis), amyotrophic lateral sclerosis (neurogenic control), and normal controls. The number of eosinophils/mm² was significantly higher in LGMD2A, PM, DM, and sIBM compared to controls but not significantly higher than other myopathies. A large overlap in the number of eosinophils/mm2 between all groups was seen. In all disease groups eosinophils were mainly found endomysially (46- 88%) and intra- and perivascularly (4-37%). There was no correlation between the numbers of eosinophils/mm² and (i) age at biopsy and (ii) the duration of the disease. The extent of myopathic, fibrotic, and inflammatory changes did not differ in samples with high and low eosinophil count. Eosinophils seem to represent an unspecific histological finding in hereditary and inflammatory myopathies, but also amyotrophic lateral sclerosis.
authors.affiliation: Department of Neurology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany;
authors.forename: Thomas
authors.initials: T
authors.lastname: Schröder
authors.affiliation: Department of Neurology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany;
authors.forename: Johann
authors.initials: J
authors.lastname: Fuchss
authors.affiliation: Department of Neurology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany;
authors.forename: Ilka
authors.initials: I
authors.lastname: Schneider
authors.affiliation: Institute of Cell Biology and Neurobiology, Charité, Universitätsmedizin Berlin, Germany.
authors.forename: Gisela
authors.initials: G
authors.lastname: Stoltenburg-Didinger
authors.affiliation: Department of Neurology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany;
authors.forename: Frank
authors.initials: F
authors.lastname: Hanisch
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D004802:Eosinophilia / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005858:Germany / Q000453:epidemiology; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D049288:Muscular Dystrophies, Limb-Girdle; D009220:Myositis / Q000453:epidemiology / Q000235:genetics*; D013194:Staining and Labeling
keywords: Eosinophil; Giemsa staining; amyotrophic lateral sclerosis; calpainopathy; hereditary myopathy; inflammatory myopathy; limb girdle muscular dystrophy