title: MicroRNA-155 inhibits migration of trophoblast cells and contributes to the pathogenesis of severe preeclampsia by regulating endothelial nitric oxide synthase.
abstract: The aim of the present study was to characterize the role of microRNA (miR)-155 in the pathogenesis of severe preeclampsia (PE). A total of 19 severe preeclampsic and 22 normal placentas were collected to measure miR-155 and endothelial nitric oxide synthase (eNOS) expression using quantitative (q)PCR and western blot analysis. The results demonstrated a significant increase in the levels of miR-155 and decreased eNOS expression in the severe preeclampsic placentas, as compared with the normal controls. In order to examine the function of miR-155 in the human placenta, the HTR8/Svneo cell line was transiently transfected with an miR-155 mimic or its inhibitor, anti-miR-155. It was confirmed that miR-155 may suppress the expression of eNOS in HTR-8/SVneo cells. Furthermore, a transwell insert invasion assay demonstrated that miR-155 inhibited cell invasion in trophoblast cells, and the effect was rescued by over expression of eNOS. The present study revealed that miR-155 has a negative regulatory role in the migratory behavior of HTR-8/SVneo cells via modulating eNOS.
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital, Xi'an Jiao Tong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Xuelan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital, Xi'an Jiao Tong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital, Xi'an Jiao Tong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital, Xi'an Jiao Tong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Gou
mesh_terms: D000328:Adult; D002460:Cell Line; D002465:Cell Movement; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D010920:Placenta / Q000201:enzymology / Q000378:metabolism; D011225:Pre-Eclampsia / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D011247:Pregnancy; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014327:Trophoblasts / Q000166:cytology; D015854:Up-Regulation
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D034741:RNA, Small Interfering; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III