title: Species differentiation within the Staphylococcus intermedius group using a refined MALDI-TOF MS database.
abstract: Among coagulase-positive staphylococci of animal origin, the members of the Staphylococcus intermedius-group (SIG: S. intermedius, Staphylococcus pseudintermedius and Staphylococcus delphini) are important opportunistic pathogens in different animal hosts and occasionally in humans. However, the unambiguous species diagnosis of SIG is often challenging. Therefore, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) -based SIG-identification with Bruker Microflex LT in combination with Biotyper 3.0 software (Bruker Daltonics, Bremen, Germany) was evaluated using (i) the original database content and (ii) the database after extension with distinct hierarchical clustered reference spectra for 60 SIG. A convenience sample comprising 200 isolates was used to compare both database performances. As a result, 17 isolates initially diagnosed as S. intermedius with the current content of the Bruker database were identified as S. pseudintermedius by applying the in-house reference spectra extended version. Furthermore, a significant improvement (average rise of log score value: 0.24) of the SIG identification score values was achieved, emphasizing that further sequence-based refinement of the Bruker database content allows improvement of MALDI-TOF MS-based identification.
authors.affiliation: Institute of Animal Hygiene and Environmental Health, Centre for Infection Medicine, Freie Universität Berlin, Berlin, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Murugaiyan
authors.forename: B
authors.initials: B
authors.lastname: Walther
authors.forename: I
authors.initials: I
authors.lastname: Stamm
authors.forename: Y
authors.initials: Y
authors.lastname: Abou-Elnaga
authors.forename: S
authors.initials: S
authors.lastname: Brueggemann-Schwarze
authors.forename: S
authors.initials: S
authors.lastname: Vincze
authors.forename: L H
authors.initials: LH
authors.lastname: Wieler
authors.forename: A
authors.initials: A
authors.lastname: Lübke-Becker
authors.forename: T
authors.initials: T
authors.lastname: Semmler
authors.forename: U
authors.initials: U
authors.lastname: Roesler
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D016208:Databases, Factual; D006801:Humans; D010802:Phylogeny; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013203:Staphylococcal Infections / Q000382:microbiology* / Q000662:veterinary; D057789:Staphylococcus intermedius / Q000145:classification* / Q000302:isolation & purification
keywords: Biotyper; Staphylococcus intermedius-group; Staphylococcus pseudintermedius; coagulase positive staphylococci; matrix-assisted laser desorption ionization-time of flight mass spectrometry; microflex