title: Posttreatment with Ma-Xing-Shi-Gan-Tang, a Chinese medicine formula, ameliorates lipopolysaccharide-induced lung microvessel hyperpermeability and inflammatory reaction in rat.
abstract: OBJECTIVE
  The aim of present study was to investigate the efficacy of MXSGT, a traditional Chinese medicine formula used for treatment of respiratory system diseases, in the LPS-induced rat ALI particularly with a focus on its effect on lung microvascular hyperpermeability and inflammatory reaction.
  
  
  METHODS
  Male Sprague-Dawley rats were injected with LPS (7.5 mg/kg, 1.5 mg/mL) intraperitoneally. MXSGT (0.52 g or 2.61 g/kg) was given by gavage six hours after LPS injection.
  
  
  RESULTS
  LPS stimulation resulted in a reduced survival rate, deteriorated vital signs, an increase in the number of leukocytes adhering to lung venules, the albumin leakage, the activity of MPO in lung tissues, the production of pro-inflammatory cytokines and lung perivascular edema. After LPS stimulation, western blot analysis revealed an increase in the expression of ICAM-1 and toll-like receptor 4, a decrease in tight junction proteins and an activation of cav-1, Src, and NF-κB. All the LPS-induced alterations were significantly attenuated by posttreatment with MXSGT.
  
  
  CONCLUSIONS
  This study demonstrated MXSGT as a potential strategy for lung microvascular hyperpermeability and inflammatory reaction in ALI, and suggested that the beneficial role of MXSGT was correlated with toll-like receptor 4, Src, and NF-κB.
authors.affiliation: Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China; Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China; Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.
authors.forename: Li-Qian
authors.initials: LQ
authors.lastname: Ma
authors.forename: Chun-Shui
authors.initials: CS
authors.lastname: Pan
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Wei
authors.forename: Ke
authors.initials: K
authors.lastname: He
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Xiao
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Fan
authors.forename: Jing-Yan
authors.initials: JY
authors.lastname: Han
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002199:Capillary Permeability / Q000187:drug effects*; D021941:Caveolae / Q000187:drug effects; D002448:Cell Adhesion; D016207:Cytokines / Q000378:metabolism; D004334:Drug Administration Schedule; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000627:therapeutic use*; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D007962:Leukocytes; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000098:blood supply*; D008297:Male; D055806:Microvessels / Q000187:drug effects* / Q000503:physiopathology; D016328:NF-kappa B / Q000378:metabolism; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D062725:Tight Junction Proteins / Q000096:biosynthesis / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis / Q000235:genetics; D014699:Venules / Q000187:drug effects / Q000503:physiopathology
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D016328:NF-kappa B; D062725:Tight Junction Proteins; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; C000591856:ma-xing-shi-gan-tang; D018799:Intercellular Adhesion Molecule-1; D016392:Proto-Oncogene Proteins pp60(c-src)
keywords: Src; caveolin-1; hyperpermeability; leukocyte; tight junction