title: Poly(lactic acid) nanoparticles coated with combined WGA and water-soluble chitosan for mucosal delivery of β-galactosidase.
abstract: A combinatorial design, physical adsorption of water-soluble chitosan (WSC) to particle surface and covalent conjugation of wheat germ agglutinin (WGA) to WSC, was applied to surface modification of poly(lactic acid) nanoparticles (NPs) for targeted delivery of β-galactosidase to the intestinal mucosa. All the surface-engineered NPs in the size range of 500-600 nm were prepared by a w/o/w solvent diffusion/evaporation technique. β-Galactosidase encapsulated in these NPs was well protected from external proteolysis and exerted high hydrolytic activity on the permeable lactose. The presence of WSC coating, whether alone or with WGA, highly improved the suspension stability of NPs and tailored the particle surface positively charged. In comparison to NPs modified with WGA or WSC alone, the synergistic action of WGA and WSC greatly enhanced the NP-mucin interactions in vitro. The highest amount of NPs was found in the small intestine at 24 h after oral administration in rats. Notably, calculated half-life of WGA-WSC-NPs in the small intestine was 6.72 h, resulting in 2.1- and 4.3-fold increase when compared to WGA-polyvinylalcohol (PVA)-NPs and WSC-NPs, much longer than that of control PVA-NPs (6.9-fold). These results suggest that NPs with the combined WGA and WSC coating represent promising candidates for efficient mucosal drug delivery as well as biomimetic treatment of lactose intolerance.
authors.affiliation: College of Chemistry and Chemical Engineering, Yantai University , Yantai , PR China and.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sheng
authors.forename: Hongjun
authors.initials: H
authors.lastname: He
authors.forename: Hui
authors.initials: H
authors.lastname: Zou
mesh_terms: D000284:Administration, Oral; D000327:Adsorption; D000818:Animals; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D004355:Drug Stability; D005079:Excipients / Q000737:chemistry; D006207:Half-Life; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D019344:Lactic Acid / Q000008:administration & dosage / Q000737:chemistry*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013535:Suspensions / Q000008:administration & dosage / Q000737:chemistry; D014867:Water / Q000008:administration & dosage / Q000737:chemistry*; D014909:Wheat Germ Agglutinins / Q000008:administration & dosage / Q000737:chemistry*; D001616:beta-Galactosidase / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D005079:Excipients; D011091:Polyesters; D011108:Polymers; D013535:Suspensions; D014909:Wheat Germ Agglutinins; D014867:Water; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan; D001616:beta-Galactosidase
keywords: Mucosal delivery; poly(lactic acid) nanoparticles; surface modification; water-soluble chitosan; wheat germ agglutinin