title: Isolation and gut microbiota modulation of antibiotic-resistant probiotics from human feces.
abstract: Antibiotic-resistant probiotics may be advantageous for antibiotic-induced gut microbiota imbalance. In this article, we aimed to isolate antibiotic-resistant bacteria as potential probiotics. Feces from 3 healthy adults and 2 infants were used to isolate the antibiotic-resistant bacteria. Then we established gut microbiota imbalance mice model by antibiotics treatment and used it to assess the effect of the probiotics. Finally, we identified 8 isolates, and 6 of them were used as probiotics cocktail. Number of anaerobe, lactobacilli, and Bifidobacterium in feces were higher in the probiotic group (9.47±0.35 log10CFU/g, 8.74±0.18 log10CFU/g, 7.24±0.38 log10CFU/g, respectively) compared with model group (P<0.05). Richness and diversity index of probiotic group (19.79±0.29 and 2.95±0.06, respectively) were larger than model group (P<0.05). Diarrhea and mucosal edema had been alleviated during probiotic treatment. Our results validated that bacteriotherapy was available to treat gut microbiota imbalance.
authors.affiliation: Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Tian
authors.affiliation: Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.affiliation: Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 510632, China. Electronic address: sunhanx718@163.com.
authors.forename: Hanxiao
authors.initials: H
authors.lastname: Sun
authors.affiliation: Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Pijin
authors.initials: P
authors.lastname: Wei
mesh_terms: D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics; D003227:Conjugation, Genetic; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D054892:Metagenome; D051379:Mice; D008826:Microbial Sensitivity Tests; D064307:Microbiota; D019936:Probiotics; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
chemical_list: D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Bacteriotherapy; Diarrhea; Gut microbiota imbalance; Probiotics cocktail