title: Streptomyces flavogriseus HS1: isolation and characterization of extracellular proteases and their compatibility with laundry detergents.
abstract: The present study describes the isolation of a new protease producing Streptomyces strain HS1 and the biochemical characterization of the secreted proteases. By sequencing of its noted 16S rDNA, HS1 strain was found to have a 100% identity with Streptomyces flavogriseus. The highest protease production was found using FermII media. In these conditions maximum protease production (99 U/mL) was obtained after 96 h incubation at 30°C and 150 rpm. HS1 strain produced at least five proteases as revealed by zymogram technique. The enzyme preparation exhibited activity over a broad range of pH (5-11) and temperature (25-70°C). Optimum activity was observed at a pH of 7.0 and a temperature of 50°C. Proteolytic activity was significantly unaffected by Ca(2+) and Mg(2+). EDTA and PMSF highly decreased the original activity. The crude extracellular proteases showed high stability when used as a detergent additive. These properties offer an interesting potential for enzymatic hydrolysis at the industrial level.
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, BP 1173, 3038 Sfax, Tunisia.
authors.forename: Sofiane
authors.identifier: 0000-0003-0277-8564
authors.initials: S
authors.lastname: Ghorbel
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, BP 1173, 3038 Sfax, Tunisia.
authors.forename: Maher
authors.initials: M
authors.lastname: Kammoun
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, BP 1173, 3038 Sfax, Tunisia.
authors.forename: Hala
authors.initials: H
authors.lastname: Soltana
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, BP 1173, 3038 Sfax, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, BP 1173, 3038 Sfax, Tunisia.
authors.forename: Noomen
authors.initials: N
authors.lastname: Hmidet
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D003902:Detergents / Q000737:chemistry*; D004795:Enzyme Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010447:Peptide Hydrolases / Q000737:chemistry / Q000302:isolation & purification; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D003902:Detergents; D010447:Peptide Hydrolases