title: Development and immunological evaluation of HLA-specific chronic myeloid leukemia polyepitope vaccine in Chinese population.
abstract: BACKGROUND
  BCR/ABL and Wilms' tumor 1 (WT1) are an ideal tumor associated antigens which can be used to develop a potential chronic myeloid leukemia (CML) dentritic cell (DC) vaccine. Here, we constructed a novel polyepitope vaccine which used recombinant lentiviral vector carrying BCR/ABL and WT1 genes, and determined the immunological effects of this vaccine in vitro.
  
  
  METHODS
  The DC vaccine was constructed using lentiviral vector transduced DCs. T lymphocytes were stimulated with DC vaccine and then co-cultured in vitro with peripheral blood mononuclear cells (PBMCs) from CML or ALL patients, respectively. The cytotoxicity of proliferous cytotoxic T lymphocytes (CTLs) was determined by the LDH assay. The IFN-γ production of CTLs was detected using ELISPOT assay.
  
  
  RESULTS
  We constructed an lentiviral vector encoding 50 different epitopes from BCR/ABL and WT1 antigens, and transferred it into DCs to prepare the DC vaccine successfully. The in vivo stimulation of CTLs with this DC vaccine were proved to show strong cytotoxicity and produce high level of IFN-γ.
  
  
  CONCLUSIONS
  The novel recombinant lentiviral polyepitope DC vaccine is a promising candidate for clinical trials and may be an effective approach for CML immunotherapy.
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Wenhao
authors.initials: W
authors.lastname: Dong
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Jingru
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Na
authors.initials: N
authors.lastname: Shao
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Jimo
authors.initials: J
authors.lastname: Jian
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Shaolei
authors.initials: S
authors.lastname: Zang
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Daoxin
authors.initials: D
authors.lastname: Ma
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan, China. Electronic address: jichunyan@sdu.edu.cn.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Ji
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology; D044466:Asian People; D019496:Cancer Vaccines / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000276:immunology*; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D007167:Immunotherapy; D007371:Interferon-gamma / Q000276:immunology; D016086:Lentivirus; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000517:prevention & control*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D025721:WT1 Proteins / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D000939:Epitopes; D025721:WT1 Proteins; D007371:Interferon-gamma; D016044:Fusion Proteins, bcr-abl
keywords: Chinese population; Chronic myeloid leukemia; Dentritic cell; Polyepitope vaccine