title: Inhibition of Polo kinase by BI2536 affects centriole separation during Drosophila male meiosis.
abstract: Pharmacological inhibition of Drosophila Polo kinase with BI2536 has allowed us to re-examine the requirements for Polo during Drosophila male gametogenesis. BI2536-treated spermatocytes persisted in a pro-metaphase state without dividing and had condensed chromosomes that did not separate. Centrosomes failed to recruit γ-tubulin and centrosomin (Cnn) and were not associated with microtubule arrays that were abnormal and did not form proper bipolar spindles. Centrioles, which usually separate during the anaphase of the first meiosis, remained held together in a V-shaped configuration suggesting that Polo kinase regulates the proteolysis that breaks centriole linkage to ensure their disengagement. Despite these defects spermatid differentiation proceeds, leading to axoneme formation.
authors.affiliation: Department of Life Sciences; University of Siena; Siena, Italy.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Riparbelli
authors.affiliation: Department of Life Sciences; University of Siena; Siena, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Gottardo
authors.affiliation: Department of Genetics; University of Cambridge; Cambridge, UK.
authors.forename: David M
authors.initials: DM
authors.lastname: Glover
authors.affiliation: Department of Life Sciences; University of Siena; Siena, Italy.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Callaini
mesh_terms: D000818:Animals; D054468:Axoneme / Q000378:metabolism / Q000648:ultrastructure; D002502:Centrioles / Q000378:metabolism*; D002842:Chromatids / Q000378:metabolism / Q000648:ultrastructure; D004330:Drosophila / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008297:Male; D008540:Meiosis / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011621:Pteridines / Q000494:pharmacology*; D011679:Pupa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism
chemical_list: C518477:BI 2536; D029721:Drosophila Proteins; D011621:Pteridines; D014404:Tubulin; C072004:polo protein, Drosophila; D017346:Protein Serine-Threonine Kinases
keywords: BI2536; Drosophila; Polo kinase; centriole disengagement; centrosome maturation; gametogenesis; spindle assembly