title: The ribosome can prevent aggregation of partially folded protein intermediates: studies using the Escherichia coli ribosome.
abstract: BACKGROUND
  Molecular chaperones that support de novo folding of proteins under non stress condition are classified as chaperone 'foldases' that are distinct from chaperone' holdases' that provide high affinity binding platform for unfolded proteins and prevent their aggregation specifically under stress conditions. Ribosome, the cellular protein synthesis machine can act as a foldase chaperone that can bind unfolded proteins and release them in folding competent state. The peptidyl transferase center (PTC) located in the domain V of the 23S rRNA of Escherichia coli ribosome (bDV RNA) is the chaperoning center of the ribosome. It has been proposed that via specific interactions between the RNA and refolding proteins, the chaperone provides information for the correct folding of unfolded polypeptide chains.
  
  
  RESULTS
  We demonstrate using Escherichia coli ribosome and variants of its domain V RNA that the ribosome can bind to partially folded intermediates of bovine carbonic anhydrase II (BCAII) and lysozyme and suppress aggregation during their refolding. Using mutants of domain V RNA we demonstrate that the time for which the chaperone retains the bound protein is an important factor in determining its ability to suppress aggregation and/or support reactivation of protein.
  
  
  CONCLUSIONS
  The ribosome can behave like a 'holdase' chaperone and has the ability to bind and hold back partially folded intermediate states of proteins from participating in the aggregation process. Since the ribosome is an essential organelle that is present in large numbers in all living cells, this ability of the ribosome provides an energetically inexpensive way to suppress cellular aggregation. Further, this ability of the ribosome might also be crucial in the context that the ribosome is one of the first chaperones to be encountered by a large nascent polypeptide chains that have a tendency to form partially folded intermediates immediately following their synthesis.
authors.affiliation: Department of Biotechnology, St. Xavier's College, Kolkata, West Bengal, India.
authors.forename: Bani Kumar
authors.initials: BK
authors.lastname: Pathak
authors.affiliation: Department of Biotechnology, St. Xavier's College, Kolkata, West Bengal, India.
authors.forename: Surojit
authors.initials: S
authors.lastname: Mondal
authors.affiliation: National Institute of Cholera and Enteric Diseases P-33, Scheme XM, Beleghata, India.
authors.forename: Amar Nath
authors.initials: AN
authors.lastname: Ghosh
authors.affiliation: Department of Biotechnology, St. Xavier's College, Kolkata, West Bengal, India.
authors.forename: Chandana
authors.initials: C
authors.lastname: Barat
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D010458:Peptidyl Transferases / Q000378:metabolism; D066329:Protein Aggregates; D058849:Protein Refolding; D012335:RNA, Ribosomal; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D066329:Protein Aggregates; D012335:RNA, Ribosomal; D010458:Peptidyl Transferases; D009113:Muramidase