title: Ultrastructural localization of adiponectin protein in vasculature of normal and atherosclerotic mice.
abstract: Adiponectin, adipose-specific secretory protein, abundantly circulates in bloodstream and its concentration is around 1000-fold higher than that of other cytokines and hormones. Hypoadiponectinemia is a risk factor for atherosclerosis. There is little or no information on ultrastructural localization of adiponectin in the vasculature. Herein we investigated the localization of vascular adiponectin in the aorta using the immunoelectron microscopic technique. In wild-type (WT) mice, adiponectin was mainly detected on the luminal surface membrane of endothelial cells (ECs) and also found intracellularly in the endocytic vesicles of ECs. In the atherosclerotic lesions of apolipoprotein E-knockout (ApoE-KO) mice, adiponectin was detected in ECs, on the cell surface membrane of synthetic smooth muscle cells, and on the surface of monocytes adherent to ECs. Changes in adiponectin localization within the wall of the aorta may provide novel insight into the pathogenesis of atherosclerosis.
authors.affiliation: 1] Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan 565-0871 [2].
authors.forename: Takuya
authors.initials: T
authors.lastname: Mori
authors.affiliation: 1] Department of Neuroscience and Cell Biology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan 565-0871 [2].
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Koyama
authors.affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan 565-0871.
authors.forename: Norikazu
authors.initials: N
authors.lastname: Maeda
authors.affiliation: Department of Neuroscience and Cell Biology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan 565-0871.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan 565-0871.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Fujishima
authors.affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan 565-0871.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Matsuda
authors.affiliation: 1] Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan 565-0871 [2] Department of Metabolism and Atherosclerosis, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan 565-0871.
authors.forename: Tohru
authors.initials: T
authors.lastname: Funahashi
authors.affiliation: Department of Neuroscience and Cell Biology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan 565-0871.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Shimada
authors.affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan 565-0871.
authors.forename: Iichiro
authors.initials: I
authors.lastname: Shimomura
mesh_terms: D052242:Adiponectin / Q000172:deficiency / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000378:metabolism; D050197:Atherosclerosis / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism*; D008297:Male; D008661:Metabolism, Inborn Errors / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009000:Monocytes / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism
chemical_list: D052242:Adiponectin; D016207:Cytokines