title: Quantitative determination of corosolic acid in rat plasma by LC-MS/MS-ESI: application to a pharmacokinetic study.
abstract: A sensitive and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of corosolic acid (CRA) in rat plasma. The plasma samples were prepared by a simple protein precipitation treatment. Chromatographic separation was performed on an Agela MG-C18 column (50 mm × 4.6 mm; i.d., 5 μm) at 30°C. The mobile phase consisting of methanol-10 mM ammonium acetate-formic acid (80:20:0.1, v/v/v; pH=3.8) was delivered with a flow rate of 0.6 ml/min and a running time of 2.5 min. Quantification was performed with positive electrospray ionization (ESI) in the multiple-reaction monitoring (MRM) mode at m/z 490 → m/z 205 for CRA, and m/z 471 → m/z 203 for TEOA (internal standard, IS), respectively. Linearity was evaluated over the concentration range of 1.00-1000 ng/ml with a lower limit of quantification (LLOQ) of 1.00 ng/ml. The validated method was successfully applied to the pharmacokinetic study of CRA after the drug was orally administered in Sprague-Dawley rats.
authors.affiliation: School of Pharmacy, Guangdong Medical College, Dongguan, PR China.
authors.forename: Baohong
authors.initials: B
authors.lastname: Li
authors.affiliation: School of Pharmacy, Guangdong Medical College, Dongguan, PR China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Nature's & US Limited Liability Corporation, St. Louis, USA.
authors.forename: Xianglu
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D014315:Triterpenes; C113861:corosolic acid