title: AtMMS21 regulates DNA damage response and homologous recombination repair in Arabidopsis.
abstract: DNA damage is a significant problem in living organisms and DNA repair pathways have been evolved in different species to maintain genomic stability. Here we demonstrated the molecular function of AtMMS21, a component of SMC5/6 complex, in plant DNA damage response. Compared with wild type, the AtMMS21 mutant plants show hypersensitivity in the DNA damaging treatments by MMS, cisplatin and gamma radiation. However, mms21-1 is not sensitive to replication blocking agents hydroxyurea and aphidicolin. The expression of a DNA damage response gene PARP2 is upregulated in mms21-1 under normal condition, suggesting that this signaling pathway is constitutively activated in the mutant. Depletion of ATAXIA-TELANGIECTASIA MUTATED (ATM) in mms21-1 enhances its root growth defect phenotype, indicating that ATM and AtMMS21 may play additive roles in DNA damage pathway. The analysis of homologous recombination frequency showed that the number of recombination events is reduced in mms21-1 mutant. Conclusively, we provided evidence that AtMMS21 plays an important role in homologous recombination for DNA damage repair.
authors.affiliation: Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Dongke
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Lai
authors.affiliation: Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Panglian
authors.initials: P
authors.lastname: Xu
authors.affiliation: Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Shengchun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Chunxin
authors.initials: C
authors.lastname: Li
authors.affiliation: Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Jinju
authors.initials: J
authors.lastname: Du
authors.affiliation: Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China. Electronic address: Yangchw@scnu.edu.cn.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Yang
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D064007:Ataxia Telangiectasia Mutated Proteins / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D059767:Recombinational DNA Repair; D015854:Up-Regulation
chemical_list: C576589:ATM protein, Arabidopsis; D029681:Arabidopsis Proteins; D011065:Poly(ADP-ribose) Polymerases; D064007:Ataxia Telangiectasia Mutated Proteins; D008025:Ligases; C555898:MMS21 protein, Arabidopsis
keywords: Arabidopsis thaliana; AtMMS21; DNA damage response; Homologous recombination