title: Selective detection and quantification of modified DNA with solid-state nanopores.
abstract: We demonstrate a solid-state nanopore assay for the unambiguous discrimination and quantification of modified DNA. Individual streptavidin proteins are employed as high-affinity tags for DNA containing a single biotin moiety. We establish that the rate of translocation events corresponds directly to relative concentration of protein-DNA complexes and use the selectivity of our approach to quantify modified oligonucleotides from among a background of unmodified DNA in solution.
authors.affiliation: Virginia Tech-Wake Forest School of Biomedical Engineering and Sciences, Wake Forest University School of Medicine , Winston Salem, North Carolina 27101, United States.
authors.forename: Autumn T
authors.initials: AT
authors.lastname: Carlsen
authors.forename: Osama K
authors.initials: OK
authors.lastname: Zahid
authors.forename: Jan A
authors.initials: JA
authors.lastname: Ruzicka
authors.forename: Ethan W
authors.initials: EW
authors.lastname: Taylor
authors.forename: Adam R
authors.initials: AR
authors.lastname: Hall
mesh_terms: D001483:Base Sequence; D019921:Biotinylation; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D055664:Electrochemical Techniques; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D058608:Nanopores / Q000648:ultrastructure*; D036103:Nanotechnology; D009841:Oligonucleotides / Q000032:analysis / Q000378:metabolism; D011506:Proteins / Q000378:metabolism
chemical_list: D009841:Oligonucleotides; D011506:Proteins; D004247:DNA
keywords: DNA; DNA modifications; Solid-state nanopores; proteins; quantification; single-molecule