title: Aptamer TY04 inhibits the growth of multiple myeloma cells via cell cycle arrest.
abstract: The aptamer TY04 is a single-stranded DNA. However, its biological function has not been elucidated. Here, we found that TY04 specifically bound to multiple myeloma cells MM.1S, and some membrane proteins on the surface of MM.1S cells constituted the target molecules of TY04. TY04 inhibited the growth of multiple myeloma cell lines, induced cell cycle arrest in mitosis, and resulted in a significant accumulation of binucleated cells. Following TY04 treatment, a concomitant increase in CDK1 and cyclin B1 expression occurred. In addition, TY04 treatment also resulted in a significant downregulation of γ-tubulin. Considering the unique advantages of aptamers, TY04 shows great potential as a drug candidate to treat multiple myeloma.
authors.affiliation: Molecular Biology Research Center, School of Life Sciences, Central South University, Changsha, 410078, China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Dai
authors.forename: Mao
authors.initials: M
authors.lastname: Ye
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Peng
authors.forename: Weihua
authors.initials: W
authors.lastname: Zhou
authors.forename: Huarong
authors.initials: H
authors.lastname: Bai
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Xiao
authors.forename: Bianying
authors.initials: B
authors.lastname: Ma
authors.forename: Jiajie
authors.initials: J
authors.lastname: Zhou
authors.forename: Shijun
authors.initials: S
authors.lastname: Tang
authors.forename: Shan
authors.initials: S
authors.lastname: Yao
authors.forename: Ye
authors.initials: Y
authors.lastname: Cao
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Qin
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D052157:Aptamers, Nucleotide / Q000494:pharmacology*; D016203:CDC2 Protein Kinase; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D056744:Cyclin B1 / Q000235:genetics; D018844:Cyclin-Dependent Kinases / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects; D009101:Multiple Myeloma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D052157:Aptamers, Nucleotide; C534699:CCNB1 protein, human; D056744:Cyclin B1; D008565:Membrane Proteins; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases