title: [RL-RVG inhibits proliferation and promotes apoptosis of lung cancer cells in vitro].
abstract: OBJECTIVE
  To infect lung adenocarcinoma A549 cells with recombinant LaSota strain Newcastle disease virus (NDV) vaccine expressing the rabies virus glycoprotein (rL-RVG), and explore the effect of rL-RVG on proliferation and apoptosis of lung cancer cells.
  
  
  METHODS
  A549 cells were infected with the rL-RVG and then detected for the expressions of RVG and NDV proteins by Western blotting. The cell proliferation was examined by MTT assay and apoptosis index and cell early apoptosis were respectively detected by TUNEL and annexin V-FITC/PI staining combined with flow cytometry. The expression of pro-apoptotic protein caspase-3 was observed by Western blotting. The LaSota strain of NDV was used as control group and PBS was the blank control.
  
  
  RESULTS
  Both RVG and NDV proteins were stably expressed in A549 cells infected with rL-RVG. MTT assay results showed that cell proliferation was significantly inhibited, and the inhibition rate of the rL-RVG group was higher than that of LaSota group. The apoptosis of A549 cells were promoted by rL-RVG infection. Flow cytometry revealed that the early apoptotic cells of the rL-RVG group increased as compared with the other two groups (P<0.05). Consistently, TUNEL assay showed the apoptotic index increased in the rL-RVG group as compared with the other two groups (P<0.05). Western blotting demonstrated that the expression of the pro-apoptotic protein caspase-3 was up-regulated. However, when we added specific broad-spectrum caspase inhibitor Z-VAD-FMK, the expression of the caspase-3 protein was obviously reduced.
  
  
  CONCLUSIONS
  The rL-RVG is stably expressed in the infected A549 cells. The rL-RVG inhibits lung cancer cell growth and promote cell apoptosis, and the effect of rL-RVG is better than the wild LaSota strain.
authors.affiliation: Department of Respiratory Medicine, Affiliated People's Hospital, Jiangsu University, Zhenjiang 212002, China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Respiratory Medicine, Affiliated People's Hospital, Jiangsu University, Zhenjiang 212002; Clinical Medicine College, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liang
authors.affiliation: Department of Respiratory Medicine, Affiliated People's Hospital, Jiangsu University, Zhenjiang 212002, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Affiliated People's Hospital, Jiangsu University, Zhenjiang 212002; Clinical Medicine College, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Respiratory Medicine, Affiliated People's Hospital, Jiangsu University, Zhenjiang 212002, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Clinical Medicine College, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D017209:Apoptosis; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D061945:Caspase Inhibitors / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D005434:Flow Cytometry; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D020287:In Situ Nick-End Labeling; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011820:Rabies virus / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D061945:Caspase Inhibitors; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; D053148:Caspase 3