title: EPA and DHA increased PPARγ expression and deceased integrin-linked kinase and integrin β1 expression in rat glomerular mesangial cells treated with lipopolysaccharide.
abstract: Fish oil containing n-3 polyunsaturated fatty acids (n-3 PUFAs) including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) is known to prevent the progression of nephropathy and retard the progression of kidney disease. This study sought to investigate the underlying mechanisms of EPA and DHA in terms of peroxisome proliferator-activated receptor γ (PPARγ), integrin-linked kinase (ILK), and integrin β1 expression in glomerular mesangial cells (GMCs) because of their critical roles in the development and progression of nephropathy. Lipopolysaccharide (LPS) significantly reduced the expression of PPARγand increased the expression of ILK at the mRNA level and at the protein level in GMCs as indicated by real-time PCR and Western blotting. In addition, LPS increased integrin β1 expression in GMCs at the mRNA level. Treatment with EPA and DHA significantly increased the expression of PPARγ and decreased the expression of ILK and integrin β1 in GMCs. These data suggest that the renoprotective effects of EPA and DHA may be related to their potential to increase the expression of PPARγ and decrease the expression of ILK and integrin β1.
authors.affiliation: Department of Pediatrics, the Second Affiliated Hospital of Shandong University.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Han
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Jiao
authors.forename: Fange
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D019012:Integrin beta1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D050527:Mesangial Cells / Q000187:drug effects / Q000378:metabolism; D047495:PPAR gamma / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats; D000099200:Scaffold Protein ILK
chemical_list: D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D019012:Integrin beta1; D008070:Lipopolysaccharides; D047495:PPAR gamma; D017346:Protein Serine-Threonine Kinases; D000099200:Scaffold Protein ILK