title: Kinetics of enzymatic hydrolysis of olive oil in batch and fed-batch systems.
abstract: This work reports experimental data, kinetic modeling, and simulations of enzyme-catalyzed hydrolysis of olive oil. This reaction was performed in batch system and an ordered-sequential Bi Bi model was used to model the kinetic mechanism. A fed-batch system was proposed and experimental data were obtained and compared to the simulated values. The kinetic model used was able to correlate the experimental data, in which a satisfactory agreement between the experimental data and modeling results was obtained under different enzyme concentration and initial free water content. Therefore, the modeling allowed a better understanding of the reaction kinetics and affords a fed-batch simulation for this system. From the results obtained, it was observed that the fed-batch approach showed to be more advantageous when compared to the conventional batch system.
authors.affiliation: Department of Chemical Engineering, Federal University of Paraná (UFPR), Curitiba, 82530-990, PR, Brazil.
authors.forename: Paloma Souza
authors.initials: PS
authors.lastname: Cabral
authors.forename: Arion Zandoná
authors.initials: AZ
authors.lastname: Filho
authors.forename: Fernando Augusto Pedersen
authors.initials: FA
authors.lastname: Voll
authors.forename: Marcos Lúcio
authors.initials: ML
authors.lastname: Corazza
mesh_terms: D019149:Bioreactors; D001709:Biotechnology; D004042:Dietary Fats, Unsaturated / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism; D004951:Esterification; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism; D008954:Models, Biological; D050178:Monoglycerides / Q000378:metabolism; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism; D014867:Water
chemical_list: D004042:Dietary Fats, Unsaturated; D004075:Diglycerides; D004800:Enzymes, Immobilized; D005230:Fatty Acids, Nonesterified; D050178:Monoglycerides; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides; D014867:Water; C093666:Lipozyme; D008049:Lipase