title: Beneficial Effects of Fractions of Nardostachys jatamansi on Lipopolysaccharide-Induced Inflammatory Response.
abstract: It has been previously shown that Nardostachys jatamansi (NJ) exhibits anti-inflammatory properties against lipopolysaccharide (LPS) challenges. However, the potency of NJ constituents against LPS-induced inflammatory responses has not been examined. In this present study, we determined which NJ extract fractions exhibit inhibitory effects against LPS-induced inflammatory responses. Among the NJ fractions, NJ-1, NJ-3, NJ-4, and NJ-6 inhibited LPS-induced production of NO. The NJ-3, NJ-4, and NJ-6 fractions also inhibited the production of cytokines, such as IL-1 β , IL-6, and TNF- α . However, NJ-1, NJ-3, NJ-4, and NJ-6 showed differential inhibitory mechanisms against LPS-induced inflammatory responses. NJ-1, NJ-3, and NJ-4 inhibited LPS-induced activation of c-jun NH2-terminal kinase (JNK) and p38 but did not affect activation of extracellular signal-regulated kinase (ERK) or NF- κ B. On the other hand, NJ-6 inhibited activation of MAPKs and NF- κ B. In addition, in vivo experiments revealed that administration of NJ-1, NJ-3, NJ-4, and NJ-6 reduced LPS-induced endotoxin shock, with NJ-6 especially showing a marked protective effect. Taken together, these results provide the evidence for the potential of selective NJ fractions against LPS-induced inflammation. Thus, it will be advantageous to further isolate and determine single effective compounds from these potent fractions.
authors.affiliation: Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea.
authors.forename: Gi-Sang
authors.identifier: 0000-0002-5627-2676
authors.initials: GS
authors.lastname: Bae
authors.affiliation: Department of Rehabilitation Medicine of Korean Medicine, Spine & Joint Center, Pusan National University Korean Medicine Hospital, Yangsan 626-770, Republic of Korea.
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Heo
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea ; BK21 plus team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea.
authors.forename: Sun Bok
authors.initials: SB
authors.lastname: Choi
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea ; BK21 plus team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea.
authors.forename: Il-Joo
authors.initials: IJ
authors.lastname: Jo
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea ; BK21 plus team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea.
authors.forename: Dong-Goo
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea ; BK21 plus team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea.
authors.forename: Joon-Yeon
authors.initials: JY
authors.lastname: Shin
authors.affiliation: Department of Beauty, Dongshin University, Naju, Jeonnam 252-17, Republic of Korea.
authors.forename: Seung-Hee
authors.initials: SH
authors.lastname: Seo
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea.
authors.forename: Kyoung-Chel
authors.initials: KC
authors.lastname: Park
authors.affiliation: Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea.
authors.forename: Dong-Sung
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea ; College of Pharmacy, Wonkwang University, Iksan 570-749, Republic of Korea.
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Oh
authors.affiliation: Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea ; College of Pharmacy, Wonkwang University, Iksan 570-749, Republic of Korea.
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea ; BK21 plus team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea.
authors.forename: Ho-Joon
authors.initials: HJ
authors.lastname: Song
authors.affiliation: Department of Rehabilitation Medicine of Korean Medicine, Spine & Joint Center, Pusan National University Korean Medicine Hospital, Yangsan 626-770, Republic of Korea.
authors.forename: Byung-Cheul
authors.initials: BC
authors.lastname: Shin
authors.affiliation: Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea ; Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea ; BK21 plus team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea.
authors.forename: Sung-Joo
authors.identifier: 0000-0002-6227-6681
authors.initials: SJ
authors.lastname: Park