title: New role for EMD (emerin), a key inner nuclear membrane protein, as an enhancer of autophagosome formation in the C16-ceramide autophagy pathway.
abstract: To date, precise roles of EMD (emerin) remain poorly described. In this paper, we investigated the role of EMD in the C16-ceramide autophagy pathway. Ceramides are bioactive signaling molecules acting notably in the regulation of cell growth, differentiation, or cell death. However, the mechanisms by which they mediate these pathways are not fully understood. We found that C16-ceramide induces EMD phosphorylation on its LEM domain through PRKACA. Upon ceramide treatment, phosphorylated EMD binds MAP1LC3B leading to an increase of autophagosome formation. These data suggest a new role of EMD as an enhancer of autophagosome formation in the C16-ceramide autophagy pathway in colon cancer cells.
authors.affiliation: GIGA-R Proteomic Unit; University of Liège; Liège, Belgium.
authors.forename: Céline
authors.initials: C
authors.lastname: Deroyer
authors.affiliation: GIGA-R Proteomic Unit; University of Liège; Liège, Belgium.
authors.forename: Anne-Françoise
authors.initials: AF
authors.lastname: Rénert
authors.affiliation: Department of Clinical Chemistry; Centre Hospitalier Universitaire de Liège; Liège, Belgium.
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Merville
authors.affiliation: GIGA-R Proteomic Unit; University of Liège; Liège, Belgium; Department of Analytical Pharmaceutical Chemistry; Department of Pharmacy; Centre Interfacultaire de Recherche du Médicament; University of Liège; Liège, Belgium.
authors.forename: Marianne
authors.initials: M
authors.lastname: Fillet
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002518:Ceramides / Q000494:pharmacology*; D045325:HCT116 Cells; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009685:Nuclear Envelope / Q000187:drug effects / Q000378:metabolism*; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D010588:Phagosomes / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D017434:Protein Structure, Tertiary; D015398:Signal Transduction / Q000187:drug effects*; D019311:Staurosporine / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: D002518:Ceramides; D007546:Isoquinolines; C502070:MAP1LC3B protein, human; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D013449:Sulfonamides; C092710:emerin; C097760:N-palmitoylsphingosine; D019311:Staurosporine; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
keywords: EMD; LEM-domain; PRKACA; autophagy; ceramide; colon cancer cells; phosphorylation