title: Preparation of a low-cost minimal medium for engineered Escherichia coli with high yield of human-like collagen II.
abstract: In order to reduce the production cost of human-like collagen (HLC), a minimal medium was introduced. On the base of Design of experiments (DOE), especially Plackett-Burman design and central composite design, a modified minimal medium that could give a high yield of HLC was developed. The optimum minimal medium for engineered E. coli BL21 ΔptsG contained 6.11g/L of glucose, 5.82g/L of (NH(4))(2)SO(4), 1.80´10(-4)g/L of thiamine and 3.00´10(-2)L of trace element solution, the other ingredients were same to that in M9 medium. And the HLC production of ptsG mutant reached to 0.26g/L in this optimized minimal medium, which approached to 0.27g/L produced by the strain without deleting ptsG gene in an optimized complex medium.
authors.affiliation: Shaanxi R&D Center of Biomaterial and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, People's Republic of China / Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an, People's Republic of China.
authors.forename: Yan'e
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Shaanxi R&D Center of Biomaterial and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, People's Republic of China / Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an, People's Republic of China.
authors.forename: Tingzhen
authors.initials: T
authors.lastname: Mu
mesh_terms: D024043:Collagen Type II / Q000096:biosynthesis*; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005818:Genetic Engineering; D006801:Humans; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics
chemical_list: D024043:Collagen Type II; D003470:Culture Media; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase