title: Size does matter! Label-free detection of small molecule-protein interaction.
abstract: This review is focused on methods for detecting small molecules and, in particular, the characterisation of their interaction with natural proteins (e.g. receptors, ion channels). Because there are intrinsic advantages to using label-free methods over labelled methods (e.g. fluorescence, radioactivity), this review only covers label-free techniques. We briefly discuss available techniques and their advantages and disadvantages, especially as related to investigating the interaction between small molecules and proteins. The reviewed techniques include well-known and widely used standard analytical methods (e.g. HPLC-MS, NMR, calorimetry, and X-ray diffraction), newer and more specialised analytical methods (e.g. biosensors), biological systems (e.g. cell lines and animal models), and in-silico approaches.
authors.affiliation: Biametrics GmbH, Auf der Morgenstelle 18, 72076, Tübingen, Germany, peter.fechner@biametrics.com.
authors.forename: Peter
authors.initials: P
authors.lastname: Fechner
authors.forename: Oliver
authors.initials: O
authors.lastname: Bleher
authors.forename: Melanie
authors.initials: M
authors.lastname: Ewald
authors.forename: Kathrin
authors.initials: K
authors.lastname: Freudenberger
authors.forename: Dominik
authors.initials: D
authors.lastname: Furin
authors.forename: Urs
authors.initials: U
authors.lastname: Hilbig
authors.forename: Felix
authors.initials: F
authors.lastname: Kolarov
authors.forename: Katrin
authors.initials: K
authors.lastname: Krieg
authors.forename: Lothar
authors.initials: L
authors.lastname: Leidner
authors.forename: Goran
authors.initials: G
authors.lastname: Markovic
authors.forename: Günther
authors.initials: G
authors.lastname: Proll
authors.forename: Florian
authors.initials: F
authors.lastname: Pröll
authors.forename: Sabrina
authors.initials: S
authors.lastname: Rau
authors.forename: Johannes
authors.initials: J
authors.lastname: Riedt
authors.forename: Barbara
authors.initials: B
authors.lastname: Schwarz
authors.forename: Patricia
authors.initials: P
authors.lastname: Weber
authors.forename: Julia
authors.initials: J
authors.lastname: Widmaier
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002853:Chromatography, Liquid; D006801:Humans; D008024:Ligands; D013058:Mass Spectrometry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D008024:Ligands; D011506:Proteins