title: Phosphorylation of CDK2 at threonine 160 regulates meiotic pachytene and diplotene progression in mice.
abstract: Telomere clustering is a widespread phenomenon among eukaryotes. However, the molecular mechanisms that regulate formation of telomere clustering in mammalian meiotic prophase I, are still largely unknown. Here, we show that CDK2, especially p39(cdk2), as a potential meiosis-specific connector interaction with SUN1 mediates formation of telomere clustering during mouse meiosis. The transition from CDK2 to p-CDK2 also regulates the progression from homologous recombination to desynapsis by interacting with MLH1. In addition, disappearance of CDK2 on the telomeres and of p-CDK2 on recombination sites, were observed in Sun1(-/-) mice and in pachytene-arrested hybrid sterile mice (pwk×C57BL/6 F1), respectively. These results suggest that transition from CDK2 to p-CDK2 plays a critical role for regulating meiosis progression.
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China; College of Life Science and Technology, Southwest University of Science and Technology, Mianyang, Sichuan 621000, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Liu
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Engineering College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Engineering College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Gendi
authors.initials: G
authors.lastname: Song
authors.affiliation: Institute of Developmental Biology and Molecular Medicine and School of Life Science, Fudan University, Shanghai 200433, China.
authors.forename: Rener
authors.initials: R
authors.lastname: Xu
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Guishuan
authors.initials: G
authors.lastname: Wang
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Li
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lian
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tian
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China. Electronic address: feisun@ustc.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism*; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D049469:Meiotic Prophase I / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D000070957:MutL Protein Homolog 1; D009687:Nuclear Proteins / Q000378:metabolism; D049471:Pachytene Stage / Q000235:genetics*; D010766:Phosphorylation; D016615:Telomere / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D008869:Microtubule-Associated Proteins; C501485:Mlh1 protein, mouse; D009687:Nuclear Proteins; C464932:SUN1 protein, mouse; C495893:Cdk2 protein, mouse; D051357:Cyclin-Dependent Kinase 2; D000070957:MutL Protein Homolog 1
keywords: MLH1; Meiosis; Mouse; Recombination; SUN1; Synapsis; Telomere clustering; p39(cdk2)/p-CDK2