title: Psoralea corylifolia L. seed extract ameliorates streptozotocin-induced diabetes in mice by inhibition of oxidative stress.
abstract: Pancreatic beta-cell death is known to be the cause of deficient insulin production in diabetes mellitus. Oxidative stress is one of the major causes of beta-cell death. In this study, we investigated the effects of Psoralea corylifolia L. seed (PCS) extract on beta-cell death. Oral administration of PCS extract resulted in a significant improvement of hyperglycemia in streptozotocin-induced diabetic mice. PCS extract treatment improved glucose tolerance and increased serum insulin levels. To study the mechanisms involved, we investigated the effects of PCS extract on H2O2-induced apoptosis in INS-1 cells. Treatment with PCS extract inhibited cell death. PCS extract treatment decreased reactive oxygen species level and activated antioxidative enzymes. Among the major components of PCS extract, psoralen and isopsoralen (coumarins), but not bakuchiol, showed preventive effects against H2O2-induced beta-cell death. These findings indicate that PCS extract may be a potential pharmacological agent to protect against pancreatic beta-cell damage caused by oxidative stress associated with diabetes.
authors.affiliation: College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon University, Incheon 406-840, Republic of Korea ; Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-840, Republic of Korea.
authors.forename: Eunhui
authors.initials: E
authors.lastname: Seo
authors.affiliation: College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon University, Incheon 406-840, Republic of Korea ; Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-840, Republic of Korea.
authors.forename: Eun-Kyu
authors.initials: EK
authors.lastname: Lee
authors.affiliation: Nanomol Inc. and Regen Phoenix R&D Institute, Incheon 406-840, Republic of Korea.
authors.forename: Cheol Soon
authors.initials: CS
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon University, Incheon 406-840, Republic of Korea.
authors.forename: Kwang-Hoon
authors.initials: KH
authors.lastname: Chun
authors.affiliation: KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon University, Incheon 406-840, Republic of Korea ; Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-840, Republic of Korea ; Gachon Medical Research Institute, Gil Hospital, Incheon 405-760, Republic of Korea.
authors.forename: Hee-Sook
authors.initials: HS
authors.lastname: Jun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental; D005363:Ficusin / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D011564:Furocoumarins / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000633:toxicity; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D029907:Psoralea / Q000737:chemistry* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000378:metabolism
chemical_list: D011564:Furocoumarins; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; C011659:angelicin; D005363:Ficusin