title: Exopolysaccharide quantification.
abstract: The extracellular (EC) matrix is a key feature of mature P. aeruginosa biofilms. Exopolysaccharides are considered as major components of this biofilm matrix. They include alginate, LPS, glucans, and psl- and pel-dependent products. Here, we describe a method of quantification of the psl-dependent mannose-rich exopolysaccharide, based on the quantification of mannose in carbohydrate-enriched cell-associated extracts and growth media. Mannose is quantified by GC or GC-MS with an internal standard, after acid hydrolysis and conversion into volatile alditol acetates.
authors.affiliation: USC ANSES-Université du Littoral-Côte d'Opale, Biochimie des Produits Aquatiques, Bd. Bassin Napoléon BP 120, Boulogne-sur-mer, 62327, France.
authors.forename: Irina
authors.initials: I
authors.lastname: Sadovskaya
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005810:Multigene Family; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013402:Sugar Alcohols / Q000378:metabolism
chemical_list: D011135:Polysaccharides, Bacterial; D013402:Sugar Alcohols