title: Effects of adenosine monophosphate-activated kinase in the ventral horn of rabbit spinal cord after transient ischemia.
abstract: OBJECTIVE
  To investigate the effect compound C, an adenosine monophosphate-activated kinase (AMPK) inhibitor, has on motor neurons of rabbit spinal cord after ischemia/reperfusion.
  
  
  METHODS
  Compound C (30 mg/kg) was administered intraperitoneally to rabbits 30 minutes before ischemia and the animals were sacrificed at 15 minutes after ischemia/reperfusion to measure lactate levels and at 72 hours after ischemia/reperfusion for morphological study.
  
  
  RESULTS
  The administration of compound C did not produce any significant changes in physiological parameters such as pH, arterial blood gas (PaCO(2) and PaO(2)), and blood glucose in rabbit either at 10 minutes before ischemia or at 10 minutes after reperfusion. However, the administration of compound C did significantly ameliorate lactate acidosis at 15 minutes after reperfusion. In addition, the administration of compound C significantly improved the neurological scores of the rabbits and reduced the neuronal death seen in the ventral horn of their spinal cords at 72 hours after ischemia/reperfusion.
  
  
  CONCLUSIONS
  Inhibition of AMPK can ameliorate the ischemia-induced neuronal death in the spinal cord via the reduction of early lactate acidosis.
authors.forename: Byung Moon
authors.initials: BM
authors.lastname: Cho
authors.forename: Woosuk
authors.initials: W
authors.lastname: Kim
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Yoo
authors.forename: Hyo Young
authors.initials: HY
authors.lastname: Jung
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Seung Myung
authors.initials: SM
authors.lastname: Moon
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000097:blood; D008297:Male; D011720:Pyrazoles / Q000494:pharmacology* / Q000627:therapeutic use; D011743:Pyrimidines / Q000494:pharmacology* / Q000627:therapeutic use; D011817:Rabbits; D020760:Spinal Cord Ischemia / Q000188:drug therapy / Q000378:metabolism*; D066151:Spinal Cord Ventral Horn / Q000187:drug effects* / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D011720:Pyrazoles; D011743:Pyrimidines; C516138:dorsomorphin; D019344:Lactic Acid; D055372:AMP-Activated Protein Kinases
keywords: Adenosine monophosphate-activated kinase; Lactate; Neuroprotection; Rabbit; Transient spinal cord ischemia