title: Litoreibacter ascidiaceicola sp. nov., isolated from the golden sea squirt Halocynthia aurantium.
abstract: A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated RSS4-C1(T), was isolated from a golden sea squirt (Halocynthia aurantium) collected from the East Sea, South Korea. The novel strain grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 1.0-3.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain RSS4-C1(T) fell within the clade comprising the type strains of species of the genus Litoreibacter . Strain RSS4-C1(T) exhibited the highest 16S rRNA gene sequence similarity (99.6%) to the type strain of Litoreibacter albidus and sequence similarities of 96.5-98.5% to type strains of other recognized species of the genus Litoreibacter. Strain RSS4-C1(T) contained Q-10 as the predominant ubiquinone and C(18 : 1)ω7c and 11-methyl-C(18 : 1)ω7c as the major fatty acids. The major polar lipids of strain RSS4-C1(T) were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain RSS4-C1(T) was 58.0 mol% and its DNA-DNA relatedness values with type strains of four species of the genus Litoreibacter were 21-34%. The differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain RSS4-C1(T) is distinct from other species of the genus Litoreibacter. On the basis of the data presented, strain RSS4-C1(T) is considered to represent a novel species of the genus Litoreibacter, for which the name Litoreibacter ascidiaceicola sp. nov. is proposed. The type strain is RSS4-C1(T) ( = KCTC 42050(T) = CECT 8539(T)).
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan 619-705, Republic of Korea.
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan 619-705, Republic of Korea.
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan 619-705, Republic of Korea.
authors.forename: Dong-Gyun
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D014561:Urochordata / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2