title: Poly(amidoamine) dendrimer nanocarriers and their aerosol formulations for siRNA delivery to the lung epithelium.
abstract: Small interfering RNA (siRNA)-based therapies have great promise in the treatment of a number of prevalent pulmonary disorders including lung cancer, asthma and cystic fibrosis. However, progress in this area has been hindered due to the lack of carriers that can efficiently deliver siRNA to lung epithelial cells, and also due to challenges in developing oral inhalation (OI) formulations for the regional administration of siRNA and their carriers to the lungs. In this work we report the ability of generation four, amine-terminated poly(amidoamine) (PAMAM) dendrimer (G4NH2)-siRNA complexes (dendriplexes) to silence the enhanced green fluorescent protein (eGFP) gene on A549 lung alveolar epithelial cells stably expressing eGFP. We also report the formulation of the dendriplexes and their aerosol characteristics in propellant-based portable OI devices. The size and gene silencing ability of the dendriplexes was seen not to be a strong function of the N/P ratio. Silencing efficiencies of up to 40% are reported. Stable dispersions of the dendriplexes encapsulated in mannitol and also in a biodegradable and water-soluble co-oligomer were prepared in hydrofluoroalkane (HFA)-based pressurized metered-dose inhalers (pMDIs). Their aerosol characteristics were very favorable, and conducive to deep lung deposition, with respirable fractions of up to 77%. Importantly, siRNA formulated as dendriplexes in pMDIs was shown to keep its integrity after the particle preparation processes, and also after long-term exposures to HFA. The relevance of this study stems from the fact that this is the first work to report the formulation of inhalable siRNA with aerosol properties suitable to deep lung deposition using pMDIs devices that are the least expensive and most widely used portable inhalers. This study is relevant because, also for the first time, it shows that siRNA-G4NH2 dendriplexes can efficiently target lung alveolar epithelial A549 cells and silence genes even after siRNA has been exposed to the propellant environment.
authors.affiliation: Department of Chemical Engineering and Materials Science, College of Engineering, Wayne State University , 5050 Anthony Wayne Drive, Detroit, Michigan 48202, United States.
authors.forename: Denise S
authors.initials: DS
authors.lastname: Conti
authors.forename: Daniel
authors.initials: D
authors.lastname: Brewer
authors.forename: Jordan
authors.initials: J
authors.lastname: Grashik
authors.forename: Sumant
authors.initials: S
authors.lastname: Avasarala
authors.forename: Sandro R P
authors.initials: SR
authors.lastname: da Rocha
mesh_terms: D000336:Aerosols / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods; D050091:Dendrimers / Q000008:administration & dosage*; D004337:Drug Carriers / Q000008:administration & dosage; D016503:Drug Delivery Systems / Q000379:methods; D004847:Epithelial Cells / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000008:administration & dosage; D006801:Humans; D008168:Lung / Q000187:drug effects*; D053758:Nanoparticles / Q000008:administration & dosage*; D010316:Particle Size; D011073:Polyamines / Q000008:administration & dosage*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D020545:Respiratory Mucosa / Q000187:drug effects*
chemical_list: D000336:Aerosols; D050091:Dendrimers; D004337:Drug Carriers; C531249:Poly(amidoamine); D011073:Polyamines; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins