title: LC/MS/MS-based quantitative assay for the secondary messenger molecule, c-di-GMP.
abstract: The secondary messenger molecule, 3',5'-cyclic diguanosine monophosphate (c-di-GMP), controls various cellular processes in bacteria. Direct measurement of intracellular concentration of c-di-GMP is fast becoming an important tool for studying prokaryotic biology. Here, we describe a comprehensive extraction protocol from live bacteria and quantitative analysis using LC/MS/MS.
authors.affiliation: Centre for Biomolecular Sciences, School of Molecular Medical Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Irie
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Parsek
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D012015:Reference Standards; D015290:Second Messenger Systems; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP