title: Whole-genome analysis of a rare human Korean G3P rotavirus strain suggests a complex evolutionary origin potentially involving reassortment events between feline and bovine rotaviruses.
abstract: A rare human rotavirus, G3P[9] strain RVA/Human-tc/KOR/CAU12-2-51/2013/G3P[9], was isolated from the stool of a 9-year-old female hospitalized with acute watery diarrhea in August 2012 in South Korea using a cell culture system, and its genome was analyzed. The complete genomic constellation of the CAU12-2-51 strain revealed a novel genotype constellation for human rotavirus, G3-P[9]-I2-R2-C2-M2-A3-N2-T3-E3-H3. Phylogenetic analysis revealed that the CAU12-2-51 strain originated from feline- and bovine-like reassortment strains. The genes encoding VP4, VP7, NSP1, NSP3, NSP4, and NSP5 were related to human/feline-like and feline rotavirus strains, whereas the remaining five genes encoding VP1, VP2, VP3, VP6, and NSP2 were related to the human/bovine-like and bovine rotavirus strains. This novel strain was identified for the first time, providing evidence of feline/bovine-to-human transmission of rotavirus. The data presented herein provide information regarding rotavirus diversity and evolution.
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
authors.forename: Sunyoung
authors.initials: S
authors.lastname: Jeong
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
authors.forename: Van Thai
authors.initials: VT
authors.lastname: Than
authors.affiliation: Department of Pediatrics, Chung-Ang University College of Medicine, Seoul, South Korea.
authors.forename: Inseok
authors.initials: I
authors.lastname: Lim
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
authors.forename: Wonyong
authors.initials: W
authors.lastname: Kim
mesh_terms: D000818:Animals; D002415:Cats; D002417:Cattle; D002648:Child; D019143:Evolution, Molecular; D005260:Female; D016679:Genome, Viral / Q000235:genetics; D023281:Genomics; D060005:Genotyping Techniques; D006801:Humans; D010802:Phylogeny; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification; D056910:Republic of Korea; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification