title: Complement receptors C5aR and C5L2 are associated with metabolic profile, sex hormones, and liver enzymes in obese women pre- and postbariatric surgery.
abstract: OBJECTIVE
  Obesity is associated with metabolic dysfunction with sex differences and chronic, low-grade inflammation.We proposed that hepatic expression of immune complement C3 related receptors (C3aR, C5aR, and C5L2) would be associated with pre- or postmenopausal status and metabolic profile in severely obese women. We hypothesized that C5L2/C5aR ratio, potentially influencing the ASP/C5L2 metabolic versus C5a/C5aR immune response, would predict metabolic profiles after weight loss surgery.
  
  
  METHODS
  Fasting plasma (hormone, lipid, and enzyme analysis) and liver biopsies (RT-PCR gene expression) were obtained from 91 women during surgery.
  
  
  RESULTS
  Hepatic C5L2 mRNA expression was elevated in pre- versus postmenopausal women (P < 0.01) and correlated positively with circulating estradiol, estrone, ApoB, ApoA1, ApoA1/B, waist circumference, age, and LDL-C (all P < 0.05).While plasma ASP was lower in pre- versus postmenopausal women (P < 0.01), the hepatic C5L2/C5aR mRNA ratio was increased (P < 0.001) and correlated positively with estrone (P < 0.01) and estradiol (P < 0.001) and negatively with circulating ApoB and liver enzymes ALT, AST, and GGT (all P < 0.05). Over 12 months postoperatively, liver enzymes in low C5L2/C5aR mRNA ratio group remained higher (ALP and ALT, P < 0.05, AST and GGT, P < 0.001 2-way-ANOVA).
  
  
  CONCLUSIONS
  C5L2-C5aR association with other mediators including estrogens may contribute to hepatic metabolic and inflammatory function.
authors.forename: Reza
authors.initials: R
authors.lastname: Rezvani
authors.forename: Jessica
authors.initials: J
authors.lastname: Smith
authors.forename: Marc
authors.initials: M
authors.lastname: Lapointe
authors.forename: Picard
authors.initials: P
authors.lastname: Marceau
authors.forename: Andre
authors.initials: A
authors.lastname: Tchernof
authors.forename: Katherine
authors.initials: K
authors.lastname: Cianflone
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000818:Animals; D001053:Apolipoproteins / Q000097:blood; D050110:Bariatric Surgery; D008078:Cholesterol, LDL / Q000097:blood; D003176:Complement C3 / Q000235:genetics / Q000378:metabolism*; D015926:Complement C3a / Q000378:metabolism; D004958:Estradiol / Q000097:blood; D004970:Estrone / Q000097:blood; D005260:Female; D012739:Gonadal Steroid Hormones / Q000097:blood*; D006801:Humans; D007249:Inflammation / Q000209:etiology / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D008593:Menopause; D008875:Middle Aged; D009765:Obesity / Q000150:complications / Q000276:immunology / Q000378:metabolism* / Q000601:surgery; D012313:RNA / Q000378:metabolism; D044087:Receptor, Anaphylatoxin C5a / Q000235:genetics / Q000378:metabolism*; D055105:Waist Circumference; D015431:Weight Loss; D055815:Young Adult
chemical_list: D001053:Apolipoproteins; D008078:Cholesterol, LDL; D003176:Complement C3; D012739:Gonadal Steroid Hormones; D044087:Receptor, Anaphylatoxin C5a; C044541:complement C3a, des-Arg-(77)-; D004970:Estrone; D004958:Estradiol; D012313:RNA; D015926:Complement C3a