title: Alpha-B-crystallin expression in human laryngeal squamous cell carcinoma tissues.
abstract: BACKGROUND
  Laryngeal squamous cell carcinoma (SCC), being an aggressive malignancy, is one of the most commonly diagnosed malignant types of head and neck SCC worldwide. The recent studies suggested that αB-crystallin might play an important role in tumorigenesis. The purpose of this study was to investigate the αB-crystallin expression level in metastatic and nonmetastatic laryngeal SCC tissues and to determine its prognostic significance.
  
  
  METHODS
  Alpha-B-crystallin expression status in metastatic, nonmetastatic laryngeal SCC, and normal tissue samples was investigated by quantitative real-time polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  We demonstrated that the expression of αB-crystallin was significantly upregulated in laryngeal SCC tumor tissue samples in comparison with the corresponding normal tissues (p < .001), although no significant association has been found between αB-crystallin expression and either the metastatic potential or the T classification of the specimens.
  
  
  CONCLUSIONS
  Although expression of αB-crystallin is not statistically correlated with neck metastases, we demonstrated that αB-crystallin is significantly overexpressed in laryngeal cancer.
authors.affiliation: Department of Otorhinolaryngology, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yilmaz
authors.affiliation: Department of Medical Genetics, Istanbul University Cerrahpasa Medical School, Istanbul, Turkey.|Molecular Biology and Genetics Department, Erzurum Technical University, Erzurum, Turkey.
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Karatas
authors.affiliation: Department of Medical Genetics, Istanbul University Cerrahpasa Medical School, Istanbul, Turkey.
authors.forename: Betul
authors.initials: B
authors.lastname: Yuceturk
authors.affiliation: Department of Otorhinolaryngology, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Dag
authors.affiliation: Department of Otorhinolaryngology, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Yener
authors.affiliation: Department of Medical Genetics, Istanbul University Cerrahpasa Medical School, Istanbul, Turkey.|Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas.|Biruni University, Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozen
mesh_terms: D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality / Q000556:secondary / Q000601:surgery; D016022:Case-Control Studies; D018572:Disease-Free Survival; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000401:mortality / Q000556:secondary / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D008198:Lymph Nodes / Q000473:pathology* / Q000601:surgery; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies; D000077195:Squamous Cell Carcinoma of Head and Neck; D016019:Survival Analysis; D038203:alpha-Crystallin B Chain / Q000235:genetics*
chemical_list: C059215:CRYAB protein, human; D038203:alpha-Crystallin B Chain
keywords: laryngeal squamous cell carcinoma; lymph node; metastasis; quantitative real-time polymerase chain reaction (qRT-PCR); αB-crystallin