title: [Molecular repair mechanisms using the Intratissue Percutaneous Electrolysis technique in patellar tendonitis].
abstract: OBJECTIVE
  To investigate the molecular mechanisms of tissue response after treatment with the Intratissue Percutaneous Electrolysis (EPI(®)) technique in collagenase-induced tendinopathy in Sprague-Dawley rats.
  
  
  METHODS
  Tendinopathy was induced by injecting 50 μg of type i collagenase into the patellar tendon of 24 Sprague Dawley rats of 7 months of age and weighting 300 g. The sample was divided into 4 groups: the control group, collagenase group, and two EPI(®) technique treatment groups of 3 and 6 mA, respectively. An EPI(®) treatment session was applied, and after 3 days, the tendons were analysed using immunoblotting and electrophoresis techniques. An analysis was also made of cytochrome C protein, Smac/Diablo, vascular endothelial growth factor and its receptor 2, as well as the nuclear transcription factor peroxisome proliferator-activated receptor gamma.
  
  
  RESULTS
  A statistically significant increase, compared to the control group, was observed in the expression of cytochrome C, Smac/Diablo, vascular endothelial growth factor, its receptor 2 and peroxisome proliferator-activated receptor gamma in the groups in which the EPI(®) technique was applied.
  
  
  CONCLUSIONS
  EPI(®) technique produces an increase in anti-inflammatory and angiogenic molecular mechanisms in collagenase-induced tendon injury in rats.
authors.affiliation: Departamento de Traumatología y Rehabilitación Deportiva, CEREDE Sports Medicine, Barcelona, España. Electronic address: drabat@cerede.es.
authors.forename: F
authors.initials: F
authors.lastname: Abat
authors.affiliation: Departamento de Fisiología, Facultad de Medicina de Valencia, Valencia, España.
authors.forename: S L
authors.initials: SL
authors.lastname: Valles
authors.affiliation: Servicio de Cirugía Ortopédica y Traumatología, Hospital de la Santa Creu i Sant Pau, Universidad Autónoma de Barcelona, Barcelona, España; Institut Català de Traumatologia i Medicina de l'Esport (ICATME)-Instituto Universitario Dexeus, Universidad Autónoma de Barcelona, Barcelona, España.
authors.forename: P E
authors.initials: PE
authors.lastname: Gelber
authors.affiliation: Departamento de Traumatología y Rehabilitación Deportiva, CEREDE Sports Medicine, Barcelona, España.
authors.forename: F
authors.initials: F
authors.lastname: Polidori
authors.affiliation: Departamento de Medicina Física y Rehabilitación, New Jersey Medical School, Newark, New Jersey, Estados Unidos de América.
authors.forename: T P
authors.initials: TP
authors.lastname: Stitik
authors.affiliation: Departamento de Fisiología, Facultad de Medicina de Valencia, Valencia, España.
authors.forename: S
authors.initials: S
authors.lastname: García-Herreros
authors.affiliation: Servicio de Cirugía Ortopédica y Traumatología, Hospital de la Santa Creu i Sant Pau, Universidad Autónoma de Barcelona, Barcelona, España; Institut Català de Traumatologia i Medicina de l'Esport (ICATME)-Instituto Universitario Dexeus, Universidad Autónoma de Barcelona, Barcelona, España.
authors.forename: J C
authors.initials: JC
authors.lastname: Monllau
authors.affiliation: Departamento de Traumatología y Rehabilitación Deportiva, CEREDE Sports Medicine, Barcelona, España.
authors.forename: J M
authors.initials: JM
authors.lastname: Sanchez-Ibánez
mesh_terms: D000818:Animals; D004599:Electric Stimulation Therapy / Q000379:methods*; D004572:Electrolysis; D051379:Mice; D017847:Patellar Ligament; D051381:Rats; D017207:Rats, Sprague-Dawley; D052256:Tendinopathy / Q000628:therapy*; D014945:Wound Healing
keywords: Electrólisis Percutánea Intratisular; Intratissue Percutaneous Electrolysis; Mecanismos moleculares; Molecular mechanisms; Regeneración; Regeneration; Tendinopathy; Tendinopatía; Tendon; Tendón