title: Intermittent fasting attenuates inflammasome activity in ischemic stroke.
abstract: Recent findings have revealed a novel inflammatory mechanism that contributes to tissue injury in cerebral ischemia mediated by multi-protein complexes termed inflammasomes. Intermittent fasting (IF) can decrease the levels of pro-inflammatory cytokines in the periphery and brain. Here we investigated the impact of IF (16h of food deprivation daily) for 4months on NLRP1 and NLRP3 inflammasome activities following cerebral ischemia. Ischemic stroke was induced in C57BL/6J mice by middle cerebral artery occlusion, followed by reperfusion (I/R). IF decreased the activation of NF-κB and MAPK signaling pathways, the expression of NLRP1 and NLRP3 inflammasome proteins, and both IL-1β and IL-18 in the ischemic brain tissue. These findings demonstrate that IF can attenuate the inflammatory response and tissue damage following ischemic stroke by a mechanism involving suppression of NLRP1 and NLRP3 inflammasome activity.
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia.
authors.forename: David Yang-Wei
authors.initials: DY
authors.lastname: Fann
authors.affiliation: School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia.
authors.forename: Tomislav
authors.initials: T
authors.lastname: Santro
authors.affiliation: School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia.
authors.forename: Silvia
authors.initials: S
authors.lastname: Manzanero
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Widiapradja
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia.
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Cheng
authors.affiliation: School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia.
authors.forename: Seung-Yoon
authors.initials: SY
authors.lastname: Lee
authors.affiliation: School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia.
authors.forename: Prasad
authors.initials: P
authors.lastname: Chunduri
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon, South Korea.
authors.forename: Dong-Gyu
authors.initials: DG
authors.lastname: Jo
authors.affiliation: Department of Physiology, Georgia Health Sciences University, Augusta, GA, USA.
authors.forename: Alexis M
authors.initials: AM
authors.lastname: Stranahan
authors.affiliation: Laboratory of Neurosciences, National Institute on Aging, Intramural Research Program, Baltimore, MD, USA.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Mattson
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; School of Pharmacy, Sungkyunkwan University, Suwon, South Korea; School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia. Electronic address: phstva@nus.edu.sg.
authors.forename: Thiruma V
authors.initials: TV
authors.lastname: Arumugam
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000150:complications; D002343:Carotid Artery, Internal; D020170:Caspase 1 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005215:Fasting / Q000502:physiology*; D058847:Inflammasomes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015424:Reperfusion; D015398:Signal Transduction / Q000502:physiology; D020521:Stroke / Q000209:etiology / Q000378:metabolism* / Q000628:therapy*
chemical_list: D016207:Cytokines; D058847:Inflammasomes; D020170:Caspase 1
keywords: Inflammasomes; Intermittent fasting; Ischemic stroke; NLRP1; NLRP3