title: Automatic pseudorotaxane formation targeting on nucleic acids using a pair of reactive oligodeoxynucleotides.
abstract: Here we report a novel method to form a pseudorotaxane architecture using only a pair of reactive oligodeoxyribonucleotides (ODNs), which we designed and synthesized, and then performed the pseudorotaxane formation reaction with both DNA and RNA oligonucleotides. The reaction proceeded smoothly without any extra reagents at 37 °C and pH 7.2, leading to the formation of a stable complex on a denaturing polyacrylamide gel. Interestingly, the pseudorotaxane was formed with the cyclized ODN reversibly by the slipping process. This new pseudorotaxane formation represents a promising method for developing new DNA nanotechnologies and antisense oligonucleotides.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN , 2-1, Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Kazumitsu
authors.initials: K
authors.lastname: Onizuka
authors.forename: Fumi
authors.initials: F
authors.lastname: Nagatsugi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
mesh_terms: D001331:Automation; D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D043862:Rotaxanes / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D009838:Oligodeoxyribonucleotides; D043862:Rotaxanes; D012313:RNA; D004247:DNA