title: Usefulness of Genotype MTBDRplus assay in acid-fast bacilli positive smear specimens in Almeria, Spain.
abstract: BACKGROUND
  The urgent need for operational research evaluating test performance in a real-world setting has been highlighted. The purpose of this study was therefore to evaluate the performance of MTBDRplus assay.
  
  
  METHODS
  According to the reference method, of the 155 clinical specimens with valid results, 147 were susceptible to rifampicin (RIF) and isoniazid (INH), with 4 being multi-drug resistant (MDR) and 4 with isolated resistance to isoniazid (INH).
  
  
  RESULTS
  The results of the MTBDRplus assay were 100% concordant for the MDR and mono-resistant to INH specimens. However, the MTBDRplus assay showed a resistance pattern to RIF in one specimen which was classified as susceptible by the reference method. The majority of the specimens (118/75.6%) were also tested using the MTBDRplus method after culture on Lowenstein-Jensen media, showing 100% agreement with the results of the test directly from the specimens. An MTBDRplus test result was available within an average of 8 days.
  
  
  CONCLUSIONS
  Overall, MTBDR results showed excellent results when compared with the reference method and achieved a significant time-reduction.
authors.affiliation: Unidad de Gestión Clínica de Biotecnología, Complejo Hospitalario Torrecárdenas, Almería, Spain.
authors.forename: Miguel José
authors.initials: MJ
authors.lastname: Martínez-Lirola
authors.affiliation: Unidad de Gestión Clínica de Biotecnología, Complejo Hospitalario Torrecárdenas, Almería, Spain. Electronic address: mariajose.munoz5@um.es.
authors.forename: María José
authors.initials: MJ
authors.lastname: Munoz-Dávila
authors.affiliation: Servicio de Microbiología, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
authors.forename: Darío
authors.initials: D
authors.lastname: García-de Viedma
authors.affiliation: Empresa Pública Hospital de Poniente, El Ejido, Almería, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Cabezas Fernández
authors.affiliation: Servicio de Análisis Clínicos (Microbiología), Hospital La Inmaculada, Huércal-Overa, Almería, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Luzón García
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002374:Catalase / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D060005:Genotyping Techniques; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D010088:Oxidoreductases / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology*; D013030:Spain / Q000453:epidemiology; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; C505805:rpoB protein, Mycobacterium tuberculosis; D010088:Oxidoreductases; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; C085257:InhA protein, Mycobacterium; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin
keywords: Comparación; Comparison; Genotype MTBDRplus; Molecular susceptibility; Reducción tiempo de respuesta; Susceptibilidad molecular; Time reduction