title: Edaravone, a free radical scavenger, improves the graft viability on liver transplantation from non-heart-beating donors in pigs.
abstract: BACKGROUND
  Although liver transplantation from non-heart-beating donors (NHBDs) is an effective way to overcome shortage of donors, primary graft nonfunction is often noted in these grafts. We have previously reported that edaravone, a free radical scavenger, has a cytoprotective effect on warm ischemia-reperfusion injury and improves the function of liver grafts from NHBDs in a rat model of ischemia-reperfusion. The purpose of this study was to investigate the effects of edaravone on liver transplantations from NHBDs.
  
  
  METHODS
  Pigs were divided into three groups: (1) a heart-beating (HB) group (n = 5), in which liver grafts were retrieved from HB donors; (2) a non-heart-beating (NHB) group (n = 4), in which liver grafts were retrieved under apnea-induced NHB conditions; and (3) an edaravone-treated (ED) group (n = 5), in which liver grafts were retrieved in the same manner as the NHB group and treated with edaravone at the time of perfusion (3 mg/L in University of Wisconsin [UW] solution), cold preservation (1 mg/L in UW solution), and after surgery (1 mg/kg/d). The grafts from all groups were transplanted after 4 hours of cold preservation.
  
  
  RESULTS
  In the ED group, the 7-day survival rate was significantly higher than that in the NHB group (80% versus 0%, P = .0042, Kaplan-Meier log-rank test). Furthermore, on histologic examination, the structure of sinusoids in the ED group was well preserved and similar to that in the HB group.
  
  
  CONCLUSIONS
  Edaravone may improve the viability of liver grafts from NHBDs.
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan. Electronic address: miyazawa_koji@yahoo.co.jp.
authors.forename: K
authors.initials: K
authors.lastname: Miyazawa
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Miyagi
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Maida
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Fujio
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kashiwadate
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: W
authors.initials: W
authors.lastname: Nakanishi
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hara
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Nakanishi
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamaya
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kawagishi
authors.affiliation: Division of Advanced Cell Transplantation, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Goto
authors.affiliation: Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ohuchi
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000494:pharmacology; D015415:Biomarkers / Q000097:blood; D050377:Cold Ischemia; D019610:Cytoprotection; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology; D006085:Graft Survival / Q000187:drug effects*; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D016031:Liver Transplantation / Q000009:adverse effects / Q000379:methods*; D008297:Male; D023421:Models, Animal; D019188:Organ Preservation Solutions / Q000494:pharmacology; D011887:Raffinose / Q000494:pharmacology; D015427:Reperfusion Injury / Q000097:blood / Q000175:diagnosis / Q000209:etiology / Q000517:prevention & control*; D013552:Swine; D013997:Time Factors; D014022:Tissue Survival / Q000187:drug effects; D020858:Tissue and Organ Harvesting / Q000009:adverse effects / Q000379:methods*; D052096:Warm Ischemia
chemical_list: D015415:Biomarkers; D016166:Free Radical Scavengers; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D000077553:Edaravone; D000983:Antipyrine