title: Antitumor effects of a polypeptide isolated from Tegillarca granosa linnaeus and the related molecular mechanism.
abstract: This study is to investigate the anticancer effects and mechanisms of Tegillarca granosa Linnaeus-1 (TG-1) on renal carcinoma OS-RC-2 cells in vitro. The proliferation of OS-RC-2 cells was evaluated under various concentrations of TG-1 using MTT assay. The apoptosis of OS-RC-2 cells was analyzed using acridine orange/ethidium bromide staining. And the cell cycle distribution of OS-RC-2 cells was detected by flow cytometry. In addition, the expression level of Ki67 mRNA was examined by RT-PCR and level of casepase-3 was examined by Western blot analysis. TG-1 incubation significantly inhibited the proliferation of renal carcinoma OS-RC-2 cells and arrested cells at G0/G1 phase (P <0.05). And TG-1 also significantly inhibited the expression of Ki67 mRNA (P<0.05). Additionally, TG-1 significantly promoted apoptosis and the expression of caspase-3 in cells (P<0.05). Moreover, the optimal effects of TG-1 was achieved at the concentration of 100 mg/L The results indicate that TG-1 has antitumor effects on renal carcinoma OS-RC-2 cells and that the underlying mechanisms may be acted through inhibiting proliferation and Ki67 mRNA expression, and promoting apoptosis and caspase-3 expression.
authors.affiliation: The Medical College, Qingdao University, 308 Ningxia Street, Qingdao, Shandong, 266003, PR China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Xu
authors.affiliation: The Medical College, Qingdao University, 308 Ningxia Street, Qingdao, Shandong, 266003, PR China.
authors.forename: Zhishang
authors.initials: Z
authors.lastname: Chang
authors.affiliation: The Garrison Command Hospital of Qingdao, Qingdao, Shandong, 266003, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pathology, Municipal Hospital of Qingdao, Qingdao, Shandong, 266003, PR China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Jiang
authors.affiliation: The Medical College, Qingdao University, 308 Ningxia Street, Qingdao, Shandong, 266003, PR China.
authors.forename: Chunbo
authors.initials: C
authors.lastname: Wang
authors.affiliation: The Medical College, Qingdao University, 308 Ningxia Street, Qingdao, Shandong, 266003, PR China.
authors.forename: Luo
authors.initials: L
authors.lastname: Xu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049893:Arcidae / Q000737:chemistry*; D053148:Caspase 3 / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics; D007680:Kidney Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010455:Peptides / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D019394:Ki-67 Antigen; D010455:Peptides; D053148:Caspase 3