title: The bacteriocin sublancin attenuates intestinal injury in young mice infected with Staphylococcus aureus.
abstract: Sublancin, a bacteriocin, has bactericidal activity against a broad spectrum of gram-positive bacteria. However, studies have not been conducted to determine its in vivo efficacy against potential pathogens. The objective of this study was to investigate the effects of sublancin in a Staphylococcus aureus (S. aureus) infected mouse model which induced intestinal injury. A total of 160, 4-week-old mice were randomly assigned to one of eight treatments. Mice in the control group were injected intraperitoneally with 0.5 mL of 0.9% saline. Mice in the other seven groups were given an intraperitoneal injection of 0.5 mL saline containing 1.0 × 10(10) colony-forming units (CFU)/mL S. aureus. Six hours after inoculation, mice in the control group were again injected with 0.5 mL of 0.9% saline. Mice in the other seven groups were injected intraperitoneally with 0.5 mL of 0.9% saline containing 0, 0.5, 1.0, 2.0, or 4.0 mg/kg body weight (BW) sublancin or 1.0 or 2.0 mg/kg BW ampicillin. The results showed that 4.0 mg/kg sublancin and 2.0 mg/kg ampicillin significantly reduced mice mortality from 55 to 10%. The height and the number of proliferated cells from the intestinal villi in the sublancin and ampicillin treated mice were higher than in the control. We conclude that sublancin has potent antibacterial activity against S. aureus. Therefore, sublancin could find use as an alternative antimicrobial agent for the treatment of gram-positive bacterial infections.
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiangfang
authors.initials: X
authors.lastname: Zeng
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Chengli
authors.initials: C
authors.lastname: Hou
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Yang
authors.forename: Xi
authors.initials: X
authors.lastname: Ma
authors.forename: Philip
authors.initials: P
authors.lastname: Thacker
authors.forename: Shiyan
authors.initials: S
authors.lastname: Qiao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001430:Bacteriocins / Q000627:therapeutic use*; D015153:Blotting, Western; D002478:Cells, Cultured; D005260:Female; D007124:Immunoenzyme Techniques; D007422:Intestines / Q000187:drug effects* / Q000293:injuries / Q000382:microbiology; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000472:pathogenicity*
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D016328:NF-kappa B; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse
keywords: Staphylococcus aureus; bacteriocin; intestine; peptide; sublancin