title: Immunoproteomic approach for identification of Ascaris suum proteins recognized by pigs with porcine ascariasis.
abstract: Ascaris suum, the causative agent of porcine ascariasis, is responsible for marked economic losses in pig farms worldwide. Despite recent advances in research, including the characterization of the genome of A. suum, knowledge about the parasite/host relationship in porcine ascariasis at the molecular level is scarce and chemotherapy is the only effective option for parasite control. The aim of this study was to identify immunogenic proteins of A. suum somatic antigens associated with the pathogenicity/survival mechanisms of the parasite, by using two-dimensional (2-D) electrophoresis, 2-D Western blot and mass spectrometry (MS). A total of 24 parasite proteins recognized by serum samples from pigs naturally infected with A. suum were identified. Most of them (23/24) were identified as being involved in parasite survival mechanisms, including functions related to energy generation (12 proteins) and redox processes (5 proteins). These results may aid the search for effective chemo-therapeutic targets in porcine ascariasis. Further studies are needed, however, to illustrate the effect of the host immune response on the survival mechanisms of A. suum.
authors.affiliation: Laboratory of Parasitology, Faculty of Pharmacy, Institute of Biomedical Research of Salamanca (IBSAL) and University of Salamanca, 37007 Salamanca, Spain. Electronic address: jglez@usal.es.
authors.forename: Javier
authors.initials: J
authors.lastname: González-Miguel
authors.affiliation: Laboratory of Parasitology, Faculty of Pharmacy, Institute of Biomedical Research of Salamanca (IBSAL) and University of Salamanca, 37007 Salamanca, Spain.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Morchón
authors.affiliation: University of Milan, Leonardo da Vinci Program, Milan, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Gussoni
authors.affiliation: University of Milan, Leonardo da Vinci Program, Milan, Italy.
authors.forename: Erika
authors.initials: E
authors.lastname: Bossetti
authors.affiliation: Laboratory of Parasitology, Faculty of Pharmacy, Institute of Biomedical Research of Salamanca (IBSAL) and University of Salamanca, 37007 Salamanca, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Hormaeche
authors.affiliation: Dipartimento di Produzioni Animali, Università degli Studi di Parma, 43100 Parma, Italy.
authors.forename: Laura Helen
authors.initials: LH
authors.lastname: Kramer
authors.affiliation: Laboratory of Parasitology, Faculty of Pharmacy, Institute of Biomedical Research of Salamanca (IBSAL) and University of Salamanca, 37007 Salamanca, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Simón
mesh_terms: D000818:Animals; D001196:Ascariasis / Q000276:immunology / Q000662:veterinary*; D017165:Ascaris suum / Q000276:immunology*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015801:Helminth Proteins / Q000276:immunology*; D007162:Immunoproteins / Q000276:immunology*; D008297:Male; D013058:Mass Spectrometry; D040901:Proteomics; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000469:parasitology
chemical_list: D015801:Helminth Proteins; D007162:Immunoproteins
keywords: Antigens; Ascaris suum; Mass spectrometry; Pigs; Soluble proteome