title: [Secretory expression of salivary ATP diphosphohydrolase (apyrase) from Aedes albopictus in Pichia pastoris].
abstract: The gene-coding mature apyrase protein from Aedes albopictus was amplified by RT-PCR and cloned in frame with the a-factor secretion signal peptide into Pichia pastoris secreting expression vector pGAPZalpha-A resulting in the pGAPZa-A-apyrase. After being linearized by Bln I restriction enzyme, the recombinant pGAPZalpha-A-apyrase was trans-formed into Pichia pastoris GS115 by electroporation. Recombinant strains pGAPZalpha-A-apyrase/GS115 were screened on YPDS plates containing Zeocin and identified by PCR. The recombinant protein of apyrase (M(r) 60000) has been expressed in the supernatant of Pichia pastoris.
authors.forename: Song-Quan
authors.initials: SQ
authors.lastname: Wu
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Wang
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Lei
authors.forename: Kai-Bo
authors.initials: KB
authors.lastname: Zhang
mesh_terms: D000330:Aedes / Q000201:enzymology*; D000818:Animals; D001081:Apyrase / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism*; D010843:Pichia / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012463:Saliva / Q000201:enzymology
chemical_list: D019476:Insect Proteins; D011994:Recombinant Proteins; D001081:Apyrase