title: Plasmocytoid dendritic cell deficit of early response to toll-like receptor 7 agonist stimulation in multiple sclerosis patients.
abstract: Plasmacytoid dendritic cells (pDCs), an important immunoregulatory population, are characterized by vigorous secretion of type I interferons (IFNs) in response to toll-like receptor (TLR) 7 and 9 stimulation. We studied the function of pDCs in multiple sclerosis (MS) patients by analysis of TLR7 responses. We assessed a pDC secretion pattern of cytokines in the short term PBMC cultures stimulated with TLR7 agonist. pDCs sorted from PBMCs of both MS patients and controls were used to assess TLR7 expression profile. TLR7 induced signaling in pDCs has been analyzed with intracellular flow cytometry. We have identified a clinically correlated significant decrease of the TLR7-induced IFN-alfa (IFNa) secretion by pDCs from MS patients. This deficit has been accompanied by insufficient intracellular phosphorylation of protein kinase Akt and a decrease of the TLR7 gene expression in MS pDCs. Our results demonstrated a selective pDC deficit in MS supporting a relationship between pDCs and mechanisms of MS.
authors.affiliation: Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Kopcinskiego 22, 90-153 Lodz, Poland.
authors.forename: Marcin P
authors.initials: MP
authors.lastname: Mycko
authors.affiliation: Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Kopcinskiego 22, 90-153 Lodz, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Cwiklinska
authors.affiliation: Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Kopcinskiego 22, 90-153 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Cichalewska
authors.affiliation: Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Kopcinskiego 22, 90-153 Lodz, Poland.
authors.forename: Mariola
authors.initials: M
authors.lastname: Matysiak
authors.affiliation: Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Kopcinskiego 22, 90-153 Lodz, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Lewkowicz
authors.affiliation: Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Kopcinskiego 22, 90-153 Lodz, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Sliwinska
authors.affiliation: Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Kopcinskiego 22, 90-153 Lodz, Poland.
authors.forename: Igor
authors.initials: I
authors.lastname: Selmaj
authors.affiliation: Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Kopcinskiego 22, 90-153 Lodz, Poland. Electronic address: kselmaj@afazja.am.lodz.pl.
authors.forename: Krzysztof W
authors.initials: KW
authors.lastname: Selmaj
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology*; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis; D042541:Intracellular Space / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D051199:Toll-Like Receptor 7 / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D016898:Interferon-alpha; D051199:Toll-Like Receptor 7; D051057:Proto-Oncogene Proteins c-akt
keywords: Autoimmunity; Immunoregulation; Multiple sclerosis; Plasmocytoid dendritic cells; Toll-like receptor; Type I interferon