title: Rapid enzyme-linked immunosorbent assay for the detection of hantavirus-specific antibodies in divergent small mammals.
abstract: We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.
authors.affiliation: Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile. kcautivor@gmail.com.
authors.forename: Karla
authors.initials: K
authors.lastname: Cautivo
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA. tony.schountz@colostate.edu.
authors.forename: Tony
authors.initials: T
authors.lastname: Schountz
authors.affiliation: Facultad de Cs Veterinarias y Pecuarias, Universidad de Chile, Santiago 8820808, Chile. mariana.acuna@gmail.com.
authors.forename: Mariana
authors.initials: M
authors.lastname: Acuña-Retamar
authors.affiliation: Laboratorio de Infectología y Virología Molecular, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago 8330024, Chile. mferres@med.puc.cl.
authors.forename: Marcela
authors.initials: M
authors.lastname: Ferrés
authors.affiliation: Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile. kcautivor@gmail.com.
authors.forename: Fernando
authors.initials: F
authors.lastname: Torres-Pérez
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D002677:Chile; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006476:Orthohantavirus / Q000276:immunology / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000662:veterinary* / Q000821:virology; D016205:Sigmodontinae / Q000821:virology*; D013997:Time Factors; D014730:Veterinary Medicine / Q000379:methods*; D014773:Virology / Q000379:methods
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral