title: [Post-transplant cell immune reconstitution in humanized NOD/SCID mice].
abstract: OBJECTIVE
  To investigate the cell immune reconstitution in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice by the transplantation of human umbilical cord blood (HUCB) CD34⁺; cells. Methods CD34⁺; cells were isolated from HUCB by magnetic activated cell sorting (MACS), and then were transplanted into NOD/SCID mice following the irradiation of sublethal doses via the lateral tail vein. Human CD45⁺; CD3⁺; CD56⁺; cell populations in the peripheral blood of mice were dynamically analyzed by flow cytometry (FCM) 4, 6, 8 and 10 weeks after transplantation. After 10 weeks, the expression of human ALU gene was detected by PCR in the bone marrow of mice, and the expressions of human CD3⁺; CD56⁺; cells were examined by immunohistochemical staining in the spleen tissues.
  
  
  RESULTS
  After irradiation, the nucleated cells and giant cells in the marrow cavity of NOD/SCID mice were reduced significantly or completely demolished. The effect of myeloablative pretreatment was ideal. Human CD45⁺; CD3⁺; CD56⁺; cells were found by FCM in the peripheral blood of all surviving mice in transplantation group 4, 6, 8, and 10 weeks after the transplantation. The population of the human lymphocytes varied over time, peaked at the 8th week, and remained at a high level later. At the 10th week, the human ALU sequence could be detected in the bone marrow of all surviving mice in transplantation group, and human CD3⁺; CD56⁺; cells could be observed in the spleen tissues. All mice which received no transplantation died within 2 weeks after irradiation.
  
  
  CONCLUSIONS
  The hu-SRC-NOD/SCID model was successfully established in irradiation-induced NOD/SCID mice by the transplantation of HUCB CD34⁺; cells, and its cell immune system was effectively rebuilt.
authors.affiliation: Department of Pathology, Affiliated Anqing Municipal Hospital, Anhui Medical College, Anqing 246003, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yang
authors.affiliation: Zunyi Medical College, Zunyi 563000, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Hu
authors.affiliation: Zunyi Medical College, Zunyi 563000, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Jia
authors.affiliation: Zunyi Medical College, Zunyi 563000, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Deng
mesh_terms: D020087:Alu Elements / Q000235:genetics; D000818:Animals; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism; D019002:CD56 Antigen / Q000276:immunology / Q000378:metabolism; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013997:Time Factors; D014181:Transplantation Immunology / Q000235:genetics / Q000276:immunology*; D014183:Transplantation, Heterologous
chemical_list: D017252:CD3 Complex; D019002:CD56 Antigen; D017493:Leukocyte Common Antigens