title: The pilot study of fibrin with temporomandibular joint derived synovial stem cells in repairing TMJ disc perforation.
abstract: TMJ disc related diseases are difficult to be cured due to the poor repair ability of the disc. TMJ-SDSCs were ideal cell sources for cartilage tissue engineering which have been widely used in hyaline cartilage regeneration. Fibrin gel has been demonstrated as a potential scaffold for neocartilage formation. The aim of this study was to repair the TMJ disc perforation using fibrin/chitosan hybrid scaffold combined with TMJ-SDSCs. Rat TMJ-SDSCs were cultured on hybrid scaffold or pure chitosan scaffolds. The cell seeding efficiency, distribution, proliferation, and chondrogenic differentiation capacity were investigated. To evaluate the in vivo repair ability of cell/scaffold construct, rat TMJ disc explants were punched with a defect to mimic TMJ disc perforation. Cell seeded scaffolds were inserted into the defect of TMJ disc explants and then were implanted subcutaneously in nude mice for 4 weeks. Results demonstrated that fibrin may improve cell seeding, proliferation, and chondrogenic induction in vitro. The in vivo experiments showed more cartilage ECM deposition in fibrin/chitosan scaffold, which suggested an enhanced reparative ability. This pilot study demonstrated that the regenerative ability of TMJ-SDSCs seeded in fibrin/chitosan scaffold could be applied for repairing TMJ disc perforation.
authors.affiliation: Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, No. 237 Luo Yu Road, Wuhan, Hubei 430079, China.
authors.forename: Yang
authors.identifier: 0000-0002-7789-6592
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Oncology Department of Oral & Maxillofacial Surgery, The First Teaching Hospital of Xinjiang Medical University, Stomatology College of Xinjiang Medical University, Stomatology Research Institute of Xinjiang Province, Urumqi, Xinjiang 830054, China.
authors.forename: Zhongcheng
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, No. 237 Luo Yu Road, Wuhan, Hubei 430079, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, No. 237 Luo Yu Road, Wuhan, Hubei 430079, China.
authors.forename: Qinggong
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, No. 237 Luo Yu Road, Wuhan, Hubei 430079, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fang
authors.affiliation: Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, No. 237 Luo Yu Road, Wuhan, Hubei 430079, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Long
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D005337:Fibrin / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008819:Mice, Nude; D010865:Pilot Projects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*; D013704:Temporomandibular Joint / Q000166:cytology* / Q000293:injuries; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005337:Fibrin; D048271:Chitosan