title: Crystallization and preliminary X-ray characterization of the dephospho-CoA kinase from Legionella pneumophila.
abstract: Dephospho-CoA kinases (DPCKs) are members of the kinase family that catalyze the final step in CoA biosynthesis. Their function is phosphorylation of the 3-hydroxyl group of the ribose using ATP as a phosphate donor. Structural changes induced by ATP binding play an important role during the DPCK catalytic cycle. In this work, DPCK from Legionella pneumophila was overexpressed in Escherichia coli. The purification, crystallization and preliminary X-ray analysis of crystals of this protein are described. The protein was crystallized in space group P21212, with unit-cell parameters a = 36.29, b = 82.20, c = 81.80 Å, using the hanging-drop vapour-diffusion method. Diffraction data were collected at 100 K and the phases were determined using the molecular-replacement method.
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei 230601, People's Republic of China.
authors.forename: Dongmin
authors.initials: D
authors.lastname: Yu
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei 230601, People's Republic of China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Life Sciences, Hefei Normal University, Hefei 230601, People's Republic of China.
authors.forename: Zhongdong
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei 230601, People's Republic of China.
authors.forename: Honghua
authors.initials: H
authors.lastname: Ge
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D016952:Legionella pneumophila / Q000201:enzymology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); C020021:dephospho-CoA kinase
keywords: Legionella pneumophila; dephospho-CoA kinase