title: A new spiroplasma isolate from the field cricket (Gryllus bimaculatus) in Taiwan.
abstract: We briefly described the morphology and transmission pathway of a Spiroplasma sp. isolated from the field cricket, Gryllus bimaculatus in Taiwan, followed by the phylogenetic analysis based on the 16S rRNA gene sequence. The cricket spiroplasma infected the hemolymph, gut, muscle tissues and tracheal cells; therefore we suggest that the pathogen invaded tissues and organs from the hemolymph through the tracheal system and the endoplasmic reticular system. Based on 16S rRNA gene sequences and the phylogeny, this spiroplasma was most closely related to Spiroplasma platyhelix (Identity=95%) isolated from the dragonfly Pachydiplax longipennis and belongs to the Ixodetis clade.
authors.affiliation: Institute of Zoology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC; Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Yu-Shin
authors.initials: YS
authors.lastname: Nai
authors.affiliation: Institute of Zoology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC.
authors.forename: Ping-Yi
authors.initials: PY
authors.lastname: Su
authors.affiliation: Institute of Zoology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC.
authors.forename: Yu-Hsiang
authors.initials: YH
authors.lastname: Hsu
authors.affiliation: Department of Leisure Business Management, De Lin Institute of Technology, Taiwan, ROC.
authors.forename: Ching-Hao
authors.initials: CH
authors.lastname: Chiang
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Jae Su
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University, No. 1, Sec. 1, Shen Nung Road, Ilan 26047, Taiwan, ROC.
authors.forename: Yue-Wen
authors.initials: YW
authors.lastname: Chen
authors.affiliation: Institute of Zoology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC; Department of Biotechnology and Animal Science, National Ilan University, No. 1, Sec. 1, Shen Nung Road, Ilan 26047, Taiwan, ROC. Electronic address: wangch@ntu.edu.tw.
authors.forename: Chung-Hsiung
authors.initials: CH
authors.lastname: Wang
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D006135:Gryllidae / Q000382:microbiology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013149:Spiroplasma / Q000235:genetics*; D013624:Taiwan
chemical_list: D004269:DNA, Bacterial
keywords: 16S rRNA; Gryllus bimaculatus; Phylogeny; Spiroplasma