title: [Construction and identification of helper-dependent adenoviral vector encoding hemagglutinin protein of swin-origin influenza virus A H1N1].
abstract: Abstract: In order to investigate immune protection against swine-origin influenza virus (S-OIV) A H1N1, the helper-dependent adenovirus vector (HDAd) system was exploited to construct recombinant HDAd encoding hemagglutinin (HA). The HA gene was synthesized and cloned to the HDAd backbone. Then, the HDAd/HA DNA molecules were transfected into 293Cre4 cells with calcium phosphate. The cells were infected by helper virus 16 hours after the transfection. The 293Cre4 cells were coinfected with HDAd/HA and the helper virus for large-scale preparation of HDAd/HA. The HDAd/HA was obtained and purified twice with CsCI density ultracentrifugation and observed morphologically under transmission electron microscope, and the expression of HA protein was analyzed with RTPCR. Recombinant HDAd/HA expressing HA protein was successfully constructed which could pave the way for in vivo investigation on immunogenicity and efficacy against S-OIV A H1N1 infection.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Yanze
authors.initials: Y
authors.lastname: Jiang
authors.forename: Nianhua
authors.initials: N
authors.lastname: Chen
authors.forename: Yuanhui
authors.initials: Y
authors.lastname: Fu
authors.forename: Wei
authors.initials: W
authors.lastname: Qiao
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.forename: Jinsheng
authors.initials: J
authors.lastname: He
mesh_terms: D000256:Adenoviridae; D002460:Cell Line; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006378:Helper Viruses; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype
chemical_list: C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus