title: Harmful effect of detergents on lipase.
abstract: In order to study effects of detergents at molecular level, we have done activity measurements of wheat germ lipase in increasing concentration of some commercial detergents. Conformational changes in protein structure using circular dichroism and fluorescence spectroscopy were studied in increasing concentration of sodium dodecyl sulfate. Our study proves that detergents may lead to loss of enzymatic activity and structure of plant enzymes. Since detergents are common source of pollution in water bodies and the water from these resources can be used in fields, our study may prove helpful in creating awareness about harmful action of detergents.
authors.affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India.
authors.forename: Sadaf
authors.initials: S
authors.lastname: Fatima
authors.forename: Rehan
authors.initials: R
authors.lastname: Ajmal
authors.forename: Gamal
authors.initials: G
authors.lastname: Badr
authors.forename: Rizwan H
authors.initials: RH
authors.lastname: Khan
mesh_terms: D003902:Detergents / Q000009:adverse effects*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D017433:Protein Structure, Secondary / Q000187:drug effects; D017434:Protein Structure, Tertiary / Q000187:drug effects; D014908:Triticum / Q000201:enzymology
chemical_list: D003902:Detergents; D008049:Lipase