title: Nanopore biosensor for label-free and real-time detection of anthrax lethal factor.
abstract: We report a label-free real-time nanopore sensing method for the detection of anthrax lethal factor, a component of the anthrax toxin, by using a complementary single-stranded DNA as a molecular probe. The method is rapid and sensitive: sub-nanomolar concentrations of the target anthrax lethal factor DNA could be detected in ∼1 min. Further, our method is selective, which can differentiate the target DNA from other single-stranded DNA molecules at the single-base resolution. This sequence-specific detection approach should find useful application in the development of nanopore sensors for the detection of other pathogens.
authors.affiliation: Department of Biological and Chemical Sciences, Illinois Institute of Technology , 3101 South Dearborn Street, Chicago, Illinois 60616, United States.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Yujing
authors.initials: Y
authors.lastname: Han
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhou
authors.forename: Guihua
authors.initials: G
authors.lastname: Wang
authors.forename: Xiyun
authors.initials: X
authors.lastname: Guan
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001408:Bacillus anthracis / Q000378:metabolism*; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D015374:Biosensing Techniques; D004269:DNA, Bacterial / Q000032:analysis* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D006460:Hemolysin Proteins / Q000737:chemistry; D058608:Nanopores; D009693:Nucleic Acid Hybridization
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D006460:Hemolysin Proteins; C030325:anthrax toxin