title: Advances in tissue section preparation for MALDI imaging MS.
abstract: Enriched by a decade of remarkable developments, matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI IMS) has witnessed a phenomenal expansion. Initially introduced for the mapping of peptides and intact proteins from mammalian tissue sections, MALDI IMS applications now extend to a wide range of molecules including peptides, lipids, metabolites and xenobiotics. Technology and methodology are quickly evolving to push the limits of the technique forward. Within a short period of time, numerous protocols and concepts have been developed and introduced in tissue section preparation, nonexhaustively including in situ tissue chemistries and solvent-free matrix depositions. Considering the past progress and current capabilities, this Review aims to cover the different aspects and challenges of tissue section preparation for MALDI IMS.
authors.affiliation: Unit of toxicology, CURML, University of Lausanne, Bugnon street 21, 1011 Lausanne, Switzerland.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Thomas
authors.forename: Pierre
authors.initials: P
authors.lastname: Chaurand
mesh_terms: D000818:Animals; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D046509:Tissue Culture Techniques