title: Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations.
abstract: We have developed a multiplex assay covering 16 microsatellite loci, amplified in four polymerase chain reaction (PCR) assays, and loaded on the ABI DNA Analyzer in two separate panels. The assay was tested on 603 individuals originating from wild populations and hatchery stocks of Atlantic sturgeon. The assay was also tested on 12 individuals of European sturgeon and appeared to be almost equally useful. The multiplex assay designed in this study can be successfully applied in studies requiring high genetic resolution, such as relatedness analysis, selective breeding programs, and stock identification of Atlantic sturgeon.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106, Warsaw, Poland, hpana@wp.pl.
authors.forename: H
authors.initials: H
authors.lastname: Panagiotopoulou
authors.forename: D
authors.initials: D
authors.lastname: Popovic
authors.forename: K
authors.initials: K
authors.lastname: Zalewska
authors.forename: P
authors.initials: P
authors.lastname: Weglenski
authors.forename: A
authors.initials: A
authors.lastname: Stankovic
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D005399:Fishes / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D005828:Genetics, Population; D060005:Genotyping Techniques; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*