title: In vitro selection of RNA aptamers that selectively bind danofloxacin.
abstract: Danofloxacin is a synthetic fluoroquinolone with broad spectrum antibacterial activity that is used for the treatment of respiratory diseases in animal husbandry. However, danofloxacin has many adverse reactions and is toxic to humans. Especially, it detrimentally affects muscle, central nerve system, peripheral nerve system, liver, and skin in those who ingest foods in which danofloxacin has accumulated. Prescreening and determination of the level of danofloxacin in foods or food products is necessary for human health. Aptamers are composing of oligonucleotides that specifically interact with target molecules. They are emerging as detection/diagnostic ligands. Here, we used the SELEX in vitro selection technology to identify specific and high-affinity RNA aptamers with 2'-fluoro-2'-deoxyribonucleotide modified pyrimidine nucleotides against danofloxacin. Selected RNA aptamers bound specifically to danofloxacin, but not to tetracycline. Truncation of RNA aptamer up to 36 mer did not comprise specificity and affinity. The truncated RNA aptamer specifically bound to target chemical, allowing the discrimination of danofloxacin from other fluoroquinolones. The isolated specific aptamer could be a potential agent used for the rapid and cost-effective detection and sensing of danofloxacin, replacing instrumental methods including the more expensive and time-consuming methods of high performance liquid chromatography and liquid chromatography/mass spectrometry.
authors.affiliation: Department of Molecular Biology, Institute of Nanosensor and Biotechnology, Dankook University, Yongin 448-701, Republic of Korea.
authors.forename: Seung Ryul
authors.initials: SR
authors.lastname: Han
authors.affiliation: Department of Chemistry and Education, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Molecular Biology, Institute of Nanosensor and Biotechnology, Dankook University, Yongin 448-701, Republic of Korea. Electronic address: SWL0208@dankook.ac.kr.
authors.forename: Seong-Wook
authors.initials: SW
authors.lastname: Lee
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D004350:Drug Residues / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D024841:Fluoroquinolones / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D009690:Nucleic Acid Conformation; D052156:SELEX Aptamer Technique / Q000379:methods*; D020349:Surface Plasmon Resonance
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D024841:Fluoroquinolones; C068581:danofloxacin
keywords: Danofloxacin; RNA aptamer; SELEX