title: Evidences of +896 A/G TLR4 polymorphism as an indicative of prevalence of complications in T2DM patients.
abstract: T2DM is today considered as world-wide health problem, with complications responsible of an enhanced mortality and morbidity. Thus, new strategies for its prevention and therapy are necessary. For this reason, the research interest has focused its attention on TLR4 and its polymorphisms, particularly the rs4986790. However, no conclusive findings have been reported until now about the role of this polymorphism in development of T2DM and its complications, even if a recent meta-analysis showed its T2DM association in Caucasians. In this study, we sought to evaluate the weight of rs4986790 polymorphism in the risk of the major T2DM complications, including 367 T2DM patients complicated for the 55.6%. Patients with A/A and A/G TLR4 genotypes showed significant differences in complication's prevalence. In particular, AG carriers had higher risk prevalence for neuropathy (P = 0.026), lower limb arteriopathy (P = 0.013), and the major cardiovascular pathologies (P = 0.017). Their cumulative risk was significant (P = 0.01), with a threefold risk to develop neuropathy, lower limb arteriopathy, and major cardiovascular events in AG cases compared to AA cases. The adjusted OR for the confounding variables was 3.788 (95% CI: 1.642-8.741). Thus, the rs4986790 polymorphism may be an indicative of prevalence of complications in T2DM patients.
authors.affiliation: Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Tukory's Street 211, 90134 Palermo, Italy.
authors.forename: Carmela Rita
authors.initials: CR
authors.lastname: Balistreri
authors.affiliation: Metabolic Diseases and Diabetology Unit, Italian National Research Center on Aging (INRCA), Ancona, Italy.
authors.forename: Anna Rita
authors.identifier: 0000-0002-9619-0181
authors.initials: AR
authors.lastname: Bonfigli
authors.affiliation: Metabolic Diseases and Diabetology Unit, Italian National Research Center on Aging (INRCA), Ancona, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Boemi
authors.affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, Ancona, Italy ; Center of Clinical Pathology and Innovative Therapy, Italian National Research Center on Aging (INRCA), Ancona, Italy.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Olivieri
authors.affiliation: Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain ; Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Barcelona, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Ceriello
authors.affiliation: Department of Cardiovascular and Metabolic Diseases, IRCCS Gruppo Multimedica, Sesto San Giovanni (MI), Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Genovese
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, CNR-ISOF and IRCCS Neuroscience, Bologna, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Franceschi
authors.affiliation: Biostatistical Center, Italian National Research Center on Aging (INRCA), Ancona, Italy.
authors.forename: Liana
authors.initials: L
authors.lastname: Spazzafumo
authors.affiliation: Biostatistical Center, Italian National Research Center on Aging (INRCA), Ancona, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Fabietti
authors.affiliation: Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Tukory's Street 211, 90134 Palermo, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Candore
authors.affiliation: Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Tukory's Street 211, 90134 Palermo, Italy.
authors.forename: Calogero
authors.identifier: 0000-0001-8004-2363
authors.initials: C
authors.lastname: Caruso
authors.affiliation: Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Tukory's Street 211, 90134 Palermo, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Lio
authors.affiliation: Experimental Models in Clinical Pathology, Italian National Research Center on Aging (INRCA), Ancona, Italy.
authors.forename: Roberto
authors.identifier: 0000-0001-8308-7663
authors.initials: R
authors.lastname: Testa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4