title: Short communication: Viable Mycobacterium avium subspecies paratuberculosis in retail artisanal Coalho cheese from Northeastern Brazil.
abstract: Mycobacterium avium ssp. paratuberculosis (MAP) is the etiologic agent of paratuberculosis and it potentially plays a role in Crohn's disease. In humans, the main route of transmission of MAP might be the intake of contaminated milk and dairy products. Considering that MAP has already been detected in many types of cheese in different counties, and that Coalho cheese is an important dairy product in northeastern Brazil, the aim of this study was to report the first detection of MAP in retail Coalho cheese in Brazil by PCR and culture. Of 30 retail Coalho cheese samples, 3 (10%) amplified fragments of a similar size to that expected (626 bp) were obtained and viable MAP was recovered by culture from 1 (3.3%) sample. The DNA from the positive culture sample was sequenced and showed 99% identity with the insertion sequence IS900 deposited in GenBank. It was possible to identify the presence of MAP-specific DNA in the analyzed samples for the first time in Brazil, and to recover viable cells from retail Coalho cheese.
authors.affiliation: Faculdade de Ciências Biológicas e da Saúde (FACISA/UNIVIÇOSA), Av. Maria de Paula Santana, 3815, Silvestre, CEP 36570-000, Viçosa, Minas Gerais, Brazil.
authors.forename: A C S
authors.initials: AC
authors.lastname: Faria
authors.affiliation: Departamento de Medicina Veterinária, Universidade Federal de Viçosa (UFV), Av. P. H. Rolfs, s/n, Campus Universitário, CEP 36570-900, Viçosa, Minas Gerais, Brazil.
authors.forename: D G G
authors.initials: DG
authors.lastname: Schwarz
authors.affiliation: Departamento de Medicina Veterinária, Universidade Federal de Viçosa (UFV), Av. P. H. Rolfs, s/n, Campus Universitário, CEP 36570-900, Viçosa, Minas Gerais, Brazil.
authors.forename: I A
authors.initials: IA
authors.lastname: Carvalho
authors.affiliation: Departamento de Medicina Veterinária, Universidade Federal de Viçosa (UFV), Av. P. H. Rolfs, s/n, Campus Universitário, CEP 36570-900, Viçosa, Minas Gerais, Brazil.
authors.forename: B B
authors.initials: BB
authors.lastname: Rocha
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária do Meio Norte/Uep Parnaíba (EMBRAPA), Br 343, km 35, Cep 64200-970, Parnaíba, Piauí, Brazil.
authors.forename: K N
authors.initials: KN
authors.lastname: De Carvalho Castro
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária Gado de Leite (EMBRAPA), Rua Eugênio do Nascimento, 610, CEP 36038-330, Juíz de Fora, MG, Brazil.
authors.forename: M R
authors.initials: MR
authors.lastname: Silva
authors.affiliation: Departamento de Medicina Veterinária, Universidade Federal de Viçosa (UFV), Av. P. H. Rolfs, s/n, Campus Universitário, CEP 36570-900, Viçosa, Minas Gerais, Brazil. Electronic address: masm@ufv.br.
authors.forename: M A S
authors.initials: MA
authors.lastname: Moreira
mesh_terms: D000818:Animals; D001938:Brazil; D002611:Cheese / Q000382:microbiology*; D015169:Colony Count, Microbial; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial
keywords: cheese; culture; paratuberculosis; viable