title: Acetylcholinesterase inhibition-based biosensor for aluminum(III) chronoamperometric determination in aqueous media.
abstract: A novel amperometric biosensor for the determination of Al(III) based on the inhibition of the enzyme acetylcholinesterase has been developed. The immobilization of the enzyme was performed on screen-printed carbon electrodes modified with gold nanoparticles. The oxidation signal of acetylthiocholine iodide enzyme substrate was affected by the presence of Al(III) ions leading to a decrease in the amperometric current. The developed system has a detection limit of 2.1 ± 0.1 μM for Al(III). The reproducibility of the method is 8.1% (n = 4). Main interferences include Mo(VI), W(VI) and Hg(II) ions. The developed method was successfully applied to the determination of Al(III) in spiked tap water . The analysis of a certified standard reference material was also carried out. Both results agree with the certified values considering the respective associated uncertainties.
authors.affiliation: University of Costa Rica, CELEQ, San Pedro de Montes de Oca, 11500-2060 San José, Costa Rica. miriam.barquero@ucr.ac.cr.
authors.forename: Miriam
authors.initials: M
authors.lastname: Barquero-Quirós
authors.affiliation: Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain. olgado@ubu.es.
authors.forename: Olga
authors.initials: O
authors.lastname: Domínguez-Renedo
authors.affiliation: Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain. malomillo@ubu.es.
authors.forename: Maria Asunción
authors.initials: MA
authors.lastname: Alonso-Lomillo
authors.affiliation: Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain. jarcos@ubu.es.
authors.forename: María Julia
authors.initials: MJ
authors.lastname: Arcos-Martínez
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D000535:Aluminum / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D014867:Water / Q000737:chemistry*
chemical_list: D002800:Cholinesterase Inhibitors; D012996:Solutions; D014867:Water; D006046:Gold; D000535:Aluminum; D000110:Acetylcholinesterase