title: [Studies on immunomodulation effect of recombinant Sj16 from Schistosoma japonicum on inflammation response of host].
abstract: OBJECTIVE
  To study the immunomodulation effect of the recombination Sj16 from Schistosoma japonicum (reSj16) on inflammation response of host.
  
  
  METHODS
  reSj16 expressed in pGEX-4T-1 was purified by GST purification kit and detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectra (MS). The immunomodulation effect of reSj16 was observed by means of dimethylbenzene inducing mouse ear edema model, carrageenan inducing rat voix pedis swell model and acetic acid inducing mouse experiment peritonitis model.
  
  
  RESULTS
  The soluble protein of reSjl6 was obtained and identified by SDS-PAGE and MS. reSjl6 1.0, 5.0, 10.0 microg/kg evidently suppressed the mouse ear edema induced by dimethyl-benzene, significantly mitigated the rat voix pedis swelling induced by carrageenan, and remarkably suppressed the increase of the capillary permeability of abdominal cavity in experiment peritonitis mouse model.
  
  
  CONCLUSIONS
  The results further prove that Sj16 may be a potential immunosuppressive molecule and may have a notable effect on immunomodulation.
authors.forename: Xi
authors.initials: X
authors.lastname: Sun
authors.forename: He-Jun
authors.initials: HJ
authors.lastname: Zhou
authors.forename: Zhi-Yue
authors.initials: ZY
authors.lastname: Lv
authors.forename: Shuang-Min
authors.initials: SM
authors.lastname: Zhang
authors.forename: Shao-Min
authors.initials: SM
authors.lastname: Hu
authors.forename: Huan-Qin
authors.initials: HQ
authors.lastname: Zheng
authors.forename: Ming-Zhao
authors.initials: MZ
authors.lastname: Feng
authors.forename: Zhong-Dao
authors.initials: ZD
authors.lastname: Wu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D015801:Helminth Proteins / Q000276:immunology / Q000627:therapeutic use*; D056747:Immunomodulation; D007249:Inflammation / Q000276:immunology* / Q000628:therapy*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000276:immunology / Q000627:therapeutic use; D012549:Schistosoma japonicum
chemical_list: D015801:Helminth Proteins; D011994:Recombinant Proteins