title: Comparison of three molecular assays for the detection of rifampin resistance in Mycobacterium tuberculosis.
abstract: BACKGROUND
  Rifampin (RIF) is the most important first-line antituberculosis drug, and resistance to this drug may result in treatment failures. We evaluated the diagnostic performances of recently introduced, molecular assays for the detection of RIF resistance.
  
  
  METHODS
  A total of 100 isolates (50 RIF resistant and 50 RIF susceptible) were studied. Their RIF resistances were determined by conventional drug-susceptibility test. These results were compared with those of three molecular assays: Xpert MTB/RIF assay (MTB is Mycobacterium tuberculosis), Sacace MTB Real-TM resistance, and AdvanSure MDR-TB GenoBlot assay (MDR is multidrug resisitant).
  
  
  RESULTS
  Sensitivities for RIF resistance detection of Xpert MTB/RIF assay, Sacace MTB Real-TM resistance, and Advansure GenoBlot assay were 94.0%, 91.8%, and 84.0%, respectively. Their specificities for RIF resistance detection were all 100%.
  
  
  CONCLUSIONS
  Three molecular assays for the detection of RIF resistance have various performances. Xpert MTB/RIF assay shows the highest sensitivity among the three molecular assays and can be an effective choice in clinical laboratories.
authors.affiliation: Department of Laboratory Medicine, Konkuk University School of Medicine, Konkuk University Hospital, Seoul, Korea.
authors.forename: Hee-Won
authors.initials: HW
authors.lastname: Moon
authors.forename: Mina
authors.initials: M
authors.lastname: Hur
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Yeo-Min
authors.initials: YM
authors.lastname: Yun
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002374:Catalase / Q000235:genetics; D012321:DNA-Directed RNA Polymerases; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D016133:Polymerase Chain Reaction; D012293:Rifampin / Q000494:pharmacology*; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; C505805:rpoB protein, Mycobacterium tuberculosis; D010088:Oxidoreductases; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; C085257:InhA protein, Mycobacterium; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin
keywords: molecular assay; resistance; rifampin; tuberculosis