title: A novel siadenovirus detected in the kidneys and liver of Gouldian finches (Erythura gouldiae).
abstract: A novel siadenovirus was found in six captive Gouldian finches (Erythrura gouldiae) in the United States and Hungary. Histopathological examination revealed inclusions in the kidney of the captive Gouldian finch in the United States, and virions morphologically consistent with adenoviruses were seen by electron microscopy. Partial sequence of the DNA-dependent DNA polymerase gene was gained by consensus PCR and sequencing in all six finches, and all proved to be identical. In one Hungarian finch, additional sequence was obtained from the DNA polymerase gene, the pre-terminal protein (pTP) gene, the 52k gene, and the hexon gene. Bayesian, maximum likelihood, and distance-based analyses showed the novel virus clusters with the siadenoviruses, and is herein referred to as Gouldian finch adenovirus 1. The genes looked at in this study had low G+C percentages, which is common in the genus Siadenovirus, and suggestive of recent host switch. The significance of this virus' presence is unknown at this time as clinical signs of positive birds varied.
authors.affiliation: College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, FL 32608, United States.
authors.forename: Heather M
authors.initials: HM
authors.lastname: Joseph
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary.
authors.forename: Mónika Z
authors.initials: MZ
authors.lastname: Ballmann
authors.affiliation: Northwest ZooPath, 654 West Main, Monroe, WA 98272, United States.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Garner
authors.affiliation: Animal Health and Nutrition Department, The Toledo Zoo, P.O. Box 140130, Toledo, OH 43614, United States.
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Hanley
authors.affiliation: Animal Health and Nutrition Department, The Toledo Zoo, P.O. Box 140130, Toledo, OH 43614, United States.
authors.forename: Ric
authors.initials: R
authors.lastname: Berlinski
authors.affiliation: Lab. of Domestic Mammal, Wildlife, and Poultry Diseases, National Food Chain Safety Office - Veterinary Diagnostic Directorate, Tábornok u. 2, 1143 Budapest, Hungary.
authors.forename: Károly
authors.initials: K
authors.lastname: Erdélyi
authors.affiliation: College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, FL 32608, United States.
authors.forename: April L
authors.initials: AL
authors.lastname: Childress
authors.affiliation: California Animal Health and Food Safety Lab, School of Veterinary Medicine, University of California-Davis, One Shields Avenue, Davis, CA 95616, United States.
authors.forename: Scott S
authors.initials: SS
authors.lastname: Fish
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Harrach
authors.affiliation: College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, FL 32608, United States. Electronic address: wellehanj@ufl.edu.
authors.forename: James F X
authors.initials: JF
authors.lastname: Wellehan
mesh_terms: D000257:Adenoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D001499:Bayes Theorem; D001715:Bird Diseases / Q000453:epidemiology / Q000821:virology*; D004256:DNA Polymerase I / Q000235:genetics; D046369:Finches / Q000821:virology*; D058507:Host Specificity; D006814:Hungary / Q000453:epidemiology; D007668:Kidney / Q000473:pathology / Q000821:virology*; D008099:Liver / Q000473:pathology / Q000821:virology*; D010802:Phylogeny; D046809:Siadenovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014481:United States / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins; D004256:DNA Polymerase I
keywords: Erythrura gouldiae; Genome sequencing; Gouldian finch; PCR; Phylogeny; Siadenovirus