title: Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors.
abstract: Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen-antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7 % (166/183 cases) [corrected] in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors.
authors.affiliation: Department of Cancer Pathology, Hokkaido University Graduate School of Medicine, N15, W7, Kita-ku, Sapporo, 060-8638, Japan.
authors.forename: Mishie
authors.initials: M
authors.lastname: Tanino
authors.forename: Toshio
authors.initials: T
authors.lastname: Sasajima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nanjo
authors.forename: Shiori
authors.initials: S
authors.lastname: Akesaka
authors.forename: Masami
authors.initials: M
authors.lastname: Kagaya
authors.forename: Taichi
authors.initials: T
authors.lastname: Kimura
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ishida
authors.forename: Masaya
authors.initials: M
authors.lastname: Oda
authors.forename: Masataka
authors.initials: M
authors.lastname: Takahashi
authors.forename: Taku
authors.initials: T
authors.lastname: Sugawara
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Yoshioka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishihara
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Akagami
authors.forename: Akiteru
authors.initials: A
authors.lastname: Goto
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Minamiya
authors.forename: Shinya
authors.initials: S
authors.lastname: Tanaka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000937:Antigen-Antibody Reactions; D014408:Biomarkers, Tumor / Q000032:analysis*; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000473:pathology; D003937:Diagnosis, Differential; D004560:Electricity; D005260:Female; D005629:Frozen Sections; D005910:Glioma / Q000175:diagnosis / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007432:Intraoperative Period; D019394:Ki-67 Antigen / Q000032:analysis*; D008223:Lymphoma / Q000175:diagnosis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen