title: In cellulo structure determination of a novel cypovirus polyhedrin.
abstract: This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of in cellulo and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.
authors.affiliation: Diamond Light Source Ltd, Harwell Oxford, Didcot OX11 0DE, England.
authors.forename: Danny
authors.initials: D
authors.lastname: Axford
authors.affiliation: Division of Structural Biology, The Welcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, England.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Ji
authors.affiliation: Diamond Light Source Ltd, Harwell Oxford, Didcot OX11 0DE, England.
authors.forename: David I
authors.initials: DI
authors.lastname: Stuart
authors.affiliation: Division of Structural Biology, The Welcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, England.
authors.forename: Geoff
authors.initials: G
authors.lastname: Sutton
mesh_terms: D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D008958:Models, Molecular; D011487:Protein Conformation; D012087:Reoviridae / Q000737:chemistry*; D017356:Synchrotrons; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D014764:Viral Proteins
keywords: data collection; in cellulo; microcrystals; viral protein