title: A quick, easy, cheap, effective, rugged, and safe sample pretreatment and liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of 33 mycotoxins in Lentinula edodes.
abstract: Lentinula edodes, one of the most cultivated edible fungi in the world, are usually neglected for mycotoxins contamination due to the initial thinking of its resistance to mycotoxingenic molds. In the present study, a sensitive and reliable liquid chromatography with tandem mass spectrometry method was developed for the simultaneous quantification of 33 mycotoxins in L. edodes. Targeted mycotoxins were extracted using a quick, easy, cheap, effective, rugged, and safe procedure without any further clean-up step, and analyzed by liquid chromatography with tandem mass spectrometry on an Agilent Poroshell 120 EC-C18 column (100 × 3 mm, 2.7 μm) with a linear gradient elution program using water containing 5 mM ammonium acetate and methanol as the mobile phase. After validation by determining linearity (R(2) > 0.99), sensitivity (LOQ ≤ 20 ng/kg), recovery (73.6-117.9%), and precision (0.8-19.5%), the established method has been successfully applied to reveal the contamination states of various mycotoxins in L. edodes. Among the 30 tested samples, 22 were contaminated by various mycotoxins with the concentration levels ranging from 3.3-28,850.7 μg/kg, predicting that the edible fungus could be infected by the mycotoxins-producing fungi. To the best of our knowledge, this is the first report about real mycotoxins contamination in L. edodes.
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China; Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Gent, Belgium.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Han
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Feng
authors.forename: Wen
authors.initials: W
authors.lastname: Shi
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhao
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Wu
authors.forename: Aibo
authors.initials: A
authors.lastname: Wu
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000191:economics / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009183:Mycotoxins / Q000737:chemistry*; D020942:Shiitake Mushrooms / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009183:Mycotoxins
keywords: Isotopically labeled internal standards; Lentinula edodes; Mycotoxins; Simultaneous quantification