title: Vitamin E and vitamin E acetate absorption from self-assembly systems under pancreas insufficiency conditions.
abstract: We determined the bioavailability of vitamin E from self-assembly structures in patients with diagnosed chronic pancreas insufficiency. Vitamin E solubilized in dispersed inverted bicontinuous cubic phase and in micellar formulation was delivered directly to the small intestine by tube-feeding. A cross-over study with randomization of 6 subjects and 2 treatments including a combined dose of 18 mg (27 IU) of vitamin E (RRR-[5,7-methyl-((2)H6)]-α-tocopherol) and 27 mg (27 IU) vitamin E acetate (RRR-[5-methyl-(2)H3]-α-tocopheryl acetate) was applied over a time period of 1 h. Plasma samples were collected for 56 h and analyzed by liquid chromatography-mass spectrometry. Appearance of labeled tocopherols originating from the treatment started at 25 h and reached Cmax (0.6-4.6 μM depending on subject) in the 7-9 h window. From the Tmax onwards, both forms of tocopherols diminished slowly to 30-50% of their maxima within 56 h. Strong inter-individual variation was observed in the plasma appearance curves (relative standard deviation varied between 38-45%). No significant discrimination was found between the absorption of free or acetylated forms of deuterated α-tocopherol confirming that application of acetylated α-tocopherol provides the same bioavailability as free α-tocopherol. This observation is valid in both dispersed inverted bicontinuous cubic phase and micellar formulations. Furthermore, since the area-under-the-curve values from cubic phase and from micellar formulations are similar, the cubic phase formulation could represent an alternative delivery system for lipophilic micronutrients in conditions or studies where polysorbate-based micelles cannot be generated.
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Kornél
authors.initials: K
authors.lastname: Nagy
authors.affiliation: Digestive System Research Unit, Institut de Recerca Vall d'Hebron; Department of Gastroenterology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (Department of Medicine), Barcelona, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Lobo
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Courtet-Compondu
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Sophie
authors.initials: S
authors.lastname: Braga-Lagache
authors.affiliation: Digestive System Research Unit, Institut de Recerca Vall d'Hebron; Department of Gastroenterology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (Department of Medicine), Barcelona, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Ramos
authors.affiliation: Digestive Diseases Unit, Hospital de Sabadell, Institut Universitari Parc Taulí, Universitat Autònoma de Barcelona, Sabadell, Spain.
authors.forename: Valentí
authors.initials: V
authors.lastname: Puig-Divi
authors.affiliation: Digestive System Research Unit, Institut de Recerca Vall d'Hebron; Department of Gastroenterology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (Department of Medicine), Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Azpiroz
authors.affiliation: Digestive System Research Unit, Institut de Recerca Vall d'Hebron; Department of Gastroenterology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (Department of Medicine), Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Juan Ramón
authors.initials: JR
authors.lastname: Malagelada
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Maurice
authors.initials: M
authors.lastname: Beaumont
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Julie
authors.initials: J
authors.lastname: Moulin
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Simone
authors.initials: S
authors.lastname: Acquistapace
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Laurent
authors.initials: L
authors.lastname: Sagalowicz
authors.affiliation: Molecular Biomarkers Core, Nestlé Institute of Health Sciences, Campus EPFL, Quartier de l'innovation, bâtiment H, CH-1015 Lausanne, Switzerland; Ecole Polytechnique Fédérale Lausanne (EPFL), Lausanne, Switzerland; Faculty of Science, Aarhus University, Aarhus, Denmark.
authors.forename: Martin
authors.initials: M
authors.lastname: Kussmann
authors.affiliation: Digestive System Research Unit, Institut de Recerca Vall d'Hebron; Department of Gastroenterology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (Department of Medicine), Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Santos
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Birgit
authors.initials: B
authors.lastname: Holst
authors.affiliation: School of Food Science and Nutrition, University of Leeds, Leeds, UK.
authors.forename: Gary
authors.initials: G
authors.lastname: Williamson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000008:administration & dosage* / Q000493:pharmacokinetics* / Q000627:therapeutic use; D001682:Biological Availability; D018592:Cross-Over Studies; D016503:Drug Delivery Systems; D004750:Enteral Nutrition; D010188:Exocrine Pancreatic Insufficiency / Q000097:blood / Q000188:drug therapy*; D006801:Humans; D007408:Intestinal Absorption; D008297:Male; D008875:Middle Aged; D014810:Vitamin E / Q000008:administration & dosage* / Q000097:blood* / Q000627:therapeutic use; D055815:Young Adult; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000097:blood / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D024502:alpha-Tocopherol