title: Identification of a novel polyomavirus in a pancreatic transplant recipient with retinal blindness and vasculitic myopathy.
abstract: BACKGROUND
  A 33 year-old pancreatic transplant recipient developed weakness, retinal blindness, and necrotic plaques on her face, scalp, and hands.
  
  
  METHODS
  A muscle biopsy was analyzed by light and electron microscopy and high-throughput nucleic acid sequencing.
  
  
  RESULTS
  The biopsy revealed microthrombosis and viral particles in swollen endothelial cell nuclei. High-throughput sequencing of nucleic acid revealed a novel polyomavirus. In situ hybridization confirmed the presence of the polyomavirus in endothelial cells at sites of myositis and cutaneous necrosis.
  
  
  CONCLUSIONS
  New Jersey polyomavirus (NJPyV-2013) is a novel polyomavirus that may have tropism for vascular endothelial cells.
authors.affiliation: Center for Infection and Immunity.
authors.forename: Nischay
authors.initials: N
authors.lastname: Mishra
authors.affiliation: Division of Infectious Diseases, Columbia University, New York, New York.
authors.forename: Marcus
authors.initials: M
authors.lastname: Pereira
authors.affiliation: Pathology and Laboratory Medicine, Rutgers University, New Brunswick, New Jersey.
authors.forename: Roy H
authors.initials: RH
authors.lastname: Rhodes
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pennsylvania.
authors.forename: Ping
authors.initials: P
authors.lastname: An
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pennsylvania.
authors.forename: James M
authors.initials: JM
authors.lastname: Pipas
authors.affiliation: Center for Infection and Immunity.
authors.forename: Komal
authors.initials: K
authors.lastname: Jain
authors.affiliation: Center for Infection and Immunity.
authors.forename: Amit
authors.initials: A
authors.lastname: Kapoor
authors.affiliation: Center for Infection and Immunity.
authors.forename: Thomas
authors.initials: T
authors.lastname: Briese
authors.affiliation: Pathology and Cell Biology, Columbia University, New York, New York.
authors.forename: Phyllis L
authors.initials: PL
authors.lastname: Faust
authors.affiliation: Center for Infection and Immunity.
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
mesh_terms: D000328:Adult; D001706:Biopsy; D001766:Blindness / Q000209:etiology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D042783:Endothelial Cells / Q000821:virology; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D017403:In Situ Hybridization; D008853:Microscopy; D008969:Molecular Sequence Data; D009132:Muscles / Q000473:pathology; D009135:Muscular Diseases / Q000821:virology*; D027601:Polyomavirus Infections / Q000821:virology*; D012173:Retinitis / Q000150:complications / Q000821:virology*; D017422:Sequence Analysis, DNA; D066027:Transplant Recipients; D014657:Vasculitis / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: immunosuppression; myositis; polyomavirus; transplantation; vasculitis; virus discovery