title: Antioxidant and antibacterial activities of Hibiscus Rosa-sinensis Linn flower extracts.
abstract: Antioxidant and antibacterial potential of different solvent extracts of locally grown Hibiscus rosa-sinensis Linn was evaluated. The antioxidant activity was assessed by estimation of total flavonoids contents, total phenolic contents, DPPH free radical scavenging activity and percentage inhibition of linoleic acid oxidation capacity. Agar disc diffusion method was used to assess antibacterial potential of crude extract of H. rosa-sinensis. The yield of the crude extracts (23.21 ± 3.67 and 18.36 ± 2.98% in 80% methanol and ethanol solvents was calculated, respectively. Methanol and ethanol extract of H. rosa-sinensis showed total phenolics 61.45 ± 3.23 and 59.31 ± 4.31 mg/100g as gallic acid equivalent, total flavonoids 53.28 ± 1.93 and 32.25±1.21 mg/100g as catechine equivalent, DPPH free radical scavenging activity 75.46±4.67 and 64.98 ± 2.11% and inhibition of linoleic acid oxidation potential 75.8 ±3.22 and 61.6 ± 2.01% respectively, was measured. Antibacterial study against three human pathogens such as staphlococus sp. Bacillus sp. and Escherichia coli showed growth inhibitory effect in the range of 12.75 ± 1.17 to 16.75 ± 2.10 mm. These results showed H. rosa-sinensis indigenous to Kallar Kahar and its allied areas bear promising medicinal values and could be used for developing herbal medicines to target oxidative stress and infectious diseases.
authors.affiliation: Department of Chemistry, Government College University, Faisalabad, Pakistan.
authors.forename: Zulfiqar Ali
authors.initials: ZA
authors.lastname: Khan
authors.affiliation: Department of Chemistry, Government College University, Faisalabad, Pakistan.
authors.forename: Syed Ali-Raza
authors.initials: SA
authors.lastname: Naqvi
authors.affiliation: Department of Chemistry, Government College University, Faisalabad, Pakistan.
authors.forename: Ammara
authors.initials: A
authors.lastname: Mukhtar
authors.affiliation: Institute of Chemistry, University of the Punjab, Lahore, Pakistan.
authors.forename: Zaib
authors.initials: Z
authors.lastname: Hussain
authors.affiliation: Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad, Pakistan.
authors.forename: Sohail Anjum
authors.initials: SA
authors.lastname: Shahzad
authors.affiliation: Department of Chemistry, Government College University, Faisalabad, Pakistan.
authors.forename: Asim
authors.initials: A
authors.lastname: Mansha
authors.affiliation: Department of Chemistry, Government College University, Faisalabad, Pakistan.
authors.forename: Matloob
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Department of Chemistry, Government College University, Faisalabad, Pakistan.
authors.forename: Ameer Fawad
authors.initials: AF
authors.lastname: Zahoor
authors.affiliation: Department of Chemistry, Government College University, Faisalabad, Pakistan.
authors.forename: Iftikhar Hussain
authors.initials: IH
authors.lastname: Bukhari
authors.forename: Muhammad Ramazan-Saeed
authors.initials: MR
authors.lastname: Ashraf-Janjua
authors.affiliation: Department of Allied Sciences and Chemical Pathology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Nasir
authors.initials: N
authors.lastname: Mahmood
authors.affiliation: Interdisciplinary Research Center in Biomedical Materials, COMSATS Institute of Information Technology, Lahore, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Yar
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D005419:Flavonoids / Q000032:analysis; D035264:Flowers; D016166:Free Radical Scavengers / Q000494:pharmacology; D031584:Hibiscus / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts