title: Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sacchari using wheat straw hydrolysates and gamma-butyrolactone.
abstract: Burkholderia sacchari DSM 17165 is able to grow and produce poly(3-hydroxybutyrate) both on hexoses and pentoses. In a previous study, wheat straw lignocellulosic hydrolysates (WSH) containing high C6 and C5 sugar concentrations were shown to be excellent carbon sources for P(3HB) production. Using a similar feeding strategy developed for P(3HB) production based on WSH, fed-batch cultures were developed aiming at the production of the copolymer P(3HB-co-4HB) (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) by B. sacchari. The ability of this strain to synthesize P(3HB-co-4HB) was first shown in shake flasks using gamma-butyrolactone (GBL) as precursor of the 4HB units. Fed-batch cultures using glucose as carbon source (control) and GBL were developed to achieve high copolymer productivities and 4HB incorporations. The attained P(3HB-co-4HB) productivity and 4HB molar% were 0.7g/(Lh) and 4.7molar%, respectively. The 4HB incorporation was improved to 6.3 and 11.8molar% by addition of 2g/L propionic and acetic acid, respectively. When WSH were used as carbon source under the same feeding conditions, the values achieved were 0.5g/(Lh) and 5.0molar%, respectively. Burkholderia sacchari, a strain able to produce biopolymers based on xylose-rich lignocellulosic hydrolysates, is for the first time reported to produce P(3HB-co-4HB) using gamma butyrolactone as precursor.
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address: teresa.cesario@tecnico.ulisboa.pt.
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Cesário
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
authors.forename: Rodrigo S
authors.initials: RS
authors.lastname: Raposo
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal; CIIEM-Centro de Investigação Interdisciplinar Egas Moniz, ISCSEM, Campus Universitário, Quinta da Granja, 2829-511 Monte de Caparica, Portugal.
authors.forename: M Catarina
authors.initials: MC
authors.lastname: M D de Almeida
authors.affiliation: Biotrend-Inovação e Engenharia em Biotecnologia, S.A., Biocant Park, Núcleo 04-Lote 2, 3060-197 Cantanhede, Portugal.
authors.forename: Frederik
authors.initials: F
authors.lastname: van Keulen
authors.affiliation: Biotrend-Inovação e Engenharia em Biotecnologia, S.A., Biocant Park, Núcleo 04-Lote 2, 3060-197 Cantanhede, Portugal.
authors.forename: Bruno S
authors.initials: BS
authors.lastname: Ferreira
authors.affiliation: Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
authors.forename: João P
authors.initials: JP
authors.lastname: Telo
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
authors.forename: M Manuela
authors.initials: MM
authors.lastname: R da Fonseca
mesh_terms: D015107:4-Butyrolactone / Q000737:chemistry*; D061249:Batch Cell Culture Techniques; D018533:Biomass; D019117:Burkholderia / Q000254:growth & development / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D006885:Hydroxybutyrates / Q000378:metabolism*; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D011091:Polyesters / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D014908:Triticum / Q000737:chemistry*; D000094122:Polyhydroxybutyrates
chemical_list: D015107:4-Butyrolactone; D002244:Carbon; D005947:Glucose; D006885:Hydroxybutyrates; D011091:Polyesters; D011108:Polymers; C068056:poly(3-hydroxybutyrate-co-4-hydroxybutyrate); D000094122:Polyhydroxybutyrates
keywords: Burkholderia sacchari; Poly(3-hydroxybutyrate-co-4-hydroxybutyrate); Wheat straw lignocellulosic hydrolysates