title: Rapid detection of encephalomyocarditis virus by one-step reverse transcription loop-mediated isothermal amplification method.
abstract: The encephalomyocarditis virus (EMCV) can cause acute myocarditis in young pigs or reproductive failure in sows. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to detect EMCV RNA. The RT-LAMP assay was highly sensitive and able to detect 2.2 × 10(-5)ng of EMCV RNA, as no cross-reaction was observed with other viruses. The RT-LAMP assay was conducted in isothermal (62 °C) conditions within 50 min. The amplified products of EMCV could be detected as ladder-like bands using agarose gel electrophoresis. This is the first report to demonstrate the application of a one-step RT-LAMP assay for the detection of EMCV. The sensitive, specific and rapid RT-LAMP assay developed in this study can be applied widely in clinical diagnosis and field surveillance of EMCV.
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, Chinese Ministry of Agriculture, Baoding, Hebei 071001, China.
authors.forename: Wanzhe
authors.initials: W
authors.lastname: Yuan
authors.affiliation: Center of Technology, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang, Hebei 050051, China.
authors.forename: Jianchang
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, Chinese Ministry of Agriculture, Baoding, Hebei 071001, China.
authors.forename: Mingtan
authors.initials: M
authors.lastname: Sun
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, Chinese Ministry of Agriculture, Baoding, Hebei 071001, China.
authors.forename: Yingshuai
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, Chinese Ministry of Agriculture, Baoding, Hebei 071001, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, Chinese Ministry of Agriculture, Baoding, Hebei 071001, China.
authors.forename: Xiuyuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, Chinese Ministry of Agriculture, Baoding, Hebei 071001, China. Electronic address: vaccine2000@126.com.
authors.forename: Jiguo
authors.initials: J
authors.lastname: Sun
mesh_terms: D000818:Animals; D018188:Cardiovirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004587:Electrophoresis, Agar Gel; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology; D013696:Temperature; D013997:Time Factors; D014730:Veterinary Medicine / Q000379:methods
keywords: Detection; Encephalomyocarditis virus; RT-LAMP; RT-PCR; Real-time