title: Inhibitory effects of lysophosphatidic acid receptor-5 on cellular functions of sarcoma cells.
abstract: Lysophosphatidic acid (LPA) is a bioactive lipid that interacts with G protein-coupled LPA receptors (LPA receptor-1 (LPA1) to LPA6). Here, we investigated the effects of LPA signaling via LPA5 on cellular functions of sarcoma cells by generating Lpar5 overexpressing and Lpar5 knockdown cells from rat osteosarcoma and malignant fibrous histiocytoma cells, respectively. The cell motility activity of Lpar5 overexpressing cells was significantly lower, while Lpar5 knockdown cells showed high cell motility, compared with respective controls. Gelatin zymography showed that LPA5 suppressed the activation of matrix metalloproteinase-2. LPA5 also inhibited the cell motility activity of endothelial cells, correlating with the expression levels of vascular endothelial growth factor genes. These results suggest that LPA signaling via LPA5 negatively regulates the cellular functions of rat sarcoma cells.
authors.affiliation: Division of Cancer Biology and Bioinformatics and.
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Araki
authors.forename: Misaho
authors.initials: M
authors.lastname: Kitayoshi
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Miku
authors.initials: M
authors.lastname: Hirane
authors.forename: Shuhei
authors.initials: S
authors.lastname: Ozaki
authors.forename: Shiori
authors.initials: S
authors.lastname: Mori
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fukushima
authors.forename: Kanya
authors.initials: K
authors.lastname: Honoki
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Tsujiuchi
mesh_terms: D000818:Animals; D019556:COS Cells; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008246:Lysophospholipids / Q000494:pharmacology; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051381:Rats; D049368:Receptors, Lysophosphatidic Acid / Q000235:genetics / Q000378:metabolism*; D012509:Sarcoma / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D008246:Lysophospholipids; D049368:Receptors, Lysophosphatidic Acid; D042461:Vascular Endothelial Growth Factor A; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; C032881:lysophosphatidic acid
keywords: LPA receptor-5; Lysophosphatidic acid; malignant fibrous histiocytoma; osteosarcoma; tumor progression