title: Liquid chromatography-tandem mass spectrometric assay for the determination of vaccarin in rat plasma: application to a pharmacokinetic study.
abstract: Vaccarin, a flavonoid glycoside, is considered one of the major active constituents of Vaccaria segetalis. A simple and specific liquid chromatography-tandem mass spectrometric method was developed and validated for quantifying vaccarin in rat plasma following intravenous dosing. Plasma samples were precipitated with methanol and separated on a Venusil-C18 analytical column (2.1 × 50 mm, 5 µm particles) with gradient elution consisting of methanol and 0.1% (v/v) formic acid as the mobile phase. The detection was performed on an Agilent Triple Quad LC/MS with electrospray ionization inlet in the positive multiple reaction monitoring mode. Good linearity was achieved over the concentration range of 12.5-25,000 ng/mL (r(2)  > 0.99). Intra- and inter-day precisions were <9.1%, and accuracy ranged from -2.8 to 8.7%. The lower limit of quantification for vaccarin was 12.5 ng/mL, and the analyte was stable under various storage conditions. This validated method was successfully applied to the preliminary pharmacokinetic studies of vaccarin following intravenous administrations of 1.21, 2.41, and 4.82 mg/kg vaccarin in rats.
authors.affiliation: The Second Hospital of Jilin University, Changchun, 130041, People's Republic of China; China Meitan General Hospital, Beijing, 100028, People's Republic of China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Yang
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.forename: Tiance
authors.initials: T
authors.lastname: Wang
authors.forename: Shudong
authors.initials: S
authors.lastname: Zhang
authors.forename: Rihao
authors.initials: R
authors.lastname: Xu
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Zhu
authors.forename: Kexiang
authors.initials: K
authors.lastname: Liu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D004355:Drug Stability; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D006027:Glycosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D005419:Flavonoids; D006027:Glycosides
keywords: LC/MS/MS; pharmacokinetic studies; semen vaccariae; vaccarin