title: Genome-wide identification and characterization of WRKY gene family in Hevea brasiliensis.
abstract: WRKY proteins constitute a large family of transcription factors. In this study, we identified 81 WRKY genes (named HbWRKY1 to HbWRKY81) in the latest rubber tree genome. Tissue-specific expression profiles showed that 74 HbWRKYs were expressed in at least one of the tissues and the other 7 genes showed very low expression in all tissues tested, which suggested that HbWRKYs took part in many cellular processes. The responses of 20 selected HbWRKYs to jasmonic acid (JA) and ethylene (ET) were analyzed in the latex. 17 HbWRKYs responded to at least one treatment, which included 15 HbWRKYs responding to JA treatment, 15 HbWRKYs to ET, which suggested that these HbWRKYs were regulated by JA and ET. We also observed that HbWRKY3, 14, and 55 bind HbSRPP promoter and activate the transcription in yeast. This study suggests that HbWRKY proteins maybe involved in the transcriptional regulation of nature rubber biosynthesis.
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
authors.forename: Hui-Liang
authors.initials: HL
authors.lastname: Li
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Guo
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Zi-Ping
authors.initials: ZP
authors.lastname: Yang
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Tang
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
authors.forename: Shi-Qing
authors.initials: SQ
authors.lastname: Peng
mesh_terms: D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D028482:Hevea / Q000235:genetics*; D007840:Latex / Q000096:biosynthesis; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D010940:Plant Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D007840:Latex; D010940:Plant Proteins; D014157:Transcription Factors
keywords: Ethylene; Hevea brasiliensis; Jasmonic acid; Small rubber particle protein; WRKY transcription factor