title: Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis.
abstract: This study was designed to investigate the effects of local delivery of adipose-derived stem cells (ADSCs) transfected with transcription factor osterix (OSX) on bone formation during distraction osteogenesis. New Zealand white rabbits (n=54) were randomly divided into three groups (18 rabbits per group). A directed cloning technique was used for the construction of recombinant plasmid pEGFP-OSX, where EGFP is the enhanced green fluorescence protein. After osteodistraction of the right mandible of all experimental rabbits, rabbits in group A were treated with ADSCs transfected with pEGFP-OSX, group B with ADSCs transfected with pEGFP-N1, and group C with physiological saline. Radiographic and histological examinations were processed after half of the animals within each group were humanely killed by injection of sodium pentothal at Week 2 or 6 after surgery. The distraction bone density was measured as its projectional bone mineral density (BMD). Three parameters were measured, namely, the thickness of new trabeculae (TNT), and the volumes of the newly generated cortical bone (NBV1) and the cancellous bone (NBV2) of the distracted regions. Good bone generation in the distraction areas was found in group A, which had the highest BMD, TNT, and NBV in the distraction zones among the groups. There was no significant difference in bone generation in the distraction areas between groups B and C. The results indicate that the transplantation of ADSCs transfected with pEGFP-OSX can effectively promote bone generation during distraction in vivo.
authors.affiliation: Department of Oral and Maxillofacial Surgery, the Second Hospital of Shandong University, Jinan 250033, China; Department of Oral and Maxillofacial Surgery, Shanghai Key Laboratory of Stomatology, the Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China; Department of Stomatology, Zhaoyuan People's Hospital, Zhaoyuan 265400, China; School of Stomatology, Shandong University, Jinan 250012, China; Department of Ultrasonography, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China.
authors.forename: Qing-guo
authors.initials: QG
authors.lastname: Lai
authors.forename: Shao-long
authors.initials: SL
authors.lastname: Sun
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Zhou
authors.forename: Chen-ping
authors.initials: CP
authors.lastname: Zhang
authors.forename: Kui-feng
authors.initials: KF
authors.lastname: Yuan
authors.forename: Zhong-jun
authors.initials: ZJ
authors.lastname: Yang
authors.forename: Sheng-lei
authors.initials: SL
authors.lastname: Luo
authors.forename: Xiao-peng
authors.initials: XP
authors.lastname: Tang
authors.forename: Jiang-bo
authors.initials: JB
authors.lastname: Ci
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003131:Combined Modality Therapy; D015316:Genetic Therapy / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008334:Mandible / Q000254:growth & development*; D010012:Osteogenesis / Q000502:physiology*; D019857:Osteogenesis, Distraction / Q000379:methods*; D011817:Rabbits; D000076182:Sp7 Transcription Factor; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection / Q000379:methods; D016896:Treatment Outcome
chemical_list: D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; D014157:Transcription Factors; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Adipose-derived stem cell (ADSC); Distraction osteogenesis; Gene transfection; Transcription factor