title: Comparative artificial neural network and partial least squares models for analysis of Metronidazole, Diloxanide, Spiramycin and Cliquinol in pharmaceutical preparations.
abstract: Metronidazole (MNZ) is a widely used antibacterial and amoebicide drug. Therefore, it is important to develop a rapid and specific analytical method for the determination of MNZ in mixture with Spiramycin (SPY), Diloxanide (DIX) and Cliquinol (CLQ) in pharmaceutical preparations. This work describes simple, sensitive and reliable six multivariate calibration methods, namely linear and nonlinear artificial neural networks preceded by genetic algorithm (GA-ANN) and principle component analysis (PCA-ANN) as well as partial least squares (PLS) either alone or preceded by genetic algorithm (GA-PLS) for UV spectrophotometric determination of MNZ, SPY, DIX and CLQ in pharmaceutical preparations with no interference of pharmaceutical additives. The results manifest the problem of nonlinearity and how models like ANN can handle it. Analytical performance of these methods was statistically validated with respect to linearity, accuracy, precision and specificity. The developed methods indicate the ability of the previously mentioned multivariate calibration models to handle and solve UV spectra of the four components' mixtures using easy and widely used UV spectrophotometer.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sinai University, El Arish, North Sinai, Egypt.
authors.forename: Mahmoud M
authors.initials: MM
authors.lastname: Elkhoudary
authors.affiliation: Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
authors.forename: Randa A
authors.initials: RA
authors.lastname: Abdel Salam
authors.affiliation: Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt. Electronic address: ghhadad@yahoo.com.
authors.forename: Ghada M
authors.initials: GM
authors.lastname: Hadad
mesh_terms: D000465:Algorithms; D000563:Amebicides / Q000737:chemistry*; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D007464:Clioquinol / Q000737:chemistry*; D005663:Furans / Q000737:chemistry*; D016018:Least-Squares Analysis; D016014:Linear Models; D008795:Metronidazole / Q000737:chemistry*; D015233:Models, Statistical; D015999:Multivariate Analysis; D016571:Neural Networks, Computer; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D015572:Spiramycin / Q000737:chemistry*; D013607:Tablets
chemical_list: D000563:Amebicides; D005663:Furans; D013607:Tablets; D008795:Metronidazole; D007464:Clioquinol; D015572:Spiramycin; C004932:diloxanide furoate
keywords: ANN; Genetic algorithm; Metronidazole; Multivariate calibration; PLS; Spectrophotometry