title: miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2.
abstract: MicroRNAs play important roles in carcinogenesis by negatively regulating the expression of target genes. Here we explore the biological function of miR-155 and the underlying mechanism in colorectal carcinoma. We validate, for the first time, that E2F2 is a direct target of miR-155 using western blot and a luciferase reporter assay and that miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2 using siRNA technology. We also found, for the first, time that E2F2 acts as a tumor suppressor in colorectal carcinoma. Overall, miR-155 plays an important role in colorectal carcinoma tumorigenesis by negative regulation of its targets including E2F2 and may be a potential therapeutic target for colorectal carcinoma treatment.
authors.affiliation: School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, People's Republic of China, litong198824@163.com.
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.forename: Jue
authors.initials: J
authors.lastname: Yang
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Lv
authors.forename: Kunmei
authors.initials: K
authors.lastname: Liu
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.forename: Yingying
authors.initials: Y
authors.lastname: Xing
authors.forename: Tao
authors.initials: T
authors.lastname: Xi
mesh_terms: D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000473:pathology*; D050703:E2F2 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D020868:Gene Silencing; D017930:Genes, Reporter; D016147:Genes, Tumor Suppressor; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D035683:MicroRNAs / Q000378:metabolism*
chemical_list: D050703:E2F2 Transcription Factor; C493761:E2F2 protein, human; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D008156:Luciferases