title: Genomic analysis of clonal eosinophils by CGH arrays reveals new genetic regions involved in chronic eosinophilia.
abstract: To assess the presence of genetic imbalances in patients with myeloproliferative neoplasms (MPNs), 38 patients with chronic eosinophilia were studied by array comparative genomic hybridization (aCGH): seven had chronic myelogenous leukaemia (CML), BCR-ABL1 positive, nine patients had myeloproliferative neoplasia Ph- (MPN-Ph-), three had a myeloid neoplasm associated with a PDGFRA rearrangement, and the remaining two cases were Lymphoproliferative T neoplasms associated with eosinophilia. In addition, 17 patients had a secondary eosinophilia and were used as controls. Eosinophilic enrichment was carried out in all cases. Genomic imbalances were found in 76% of all MPN patients. Losses on 20q were the most frequent genetic abnormality in MPNs (32%), affected the three types of MPN studied. This study also found losses at 11q13.3 in 26% of patients with MPN-Ph- and in 19p13.11 in two of the three patients with an MPN associated with a PDGFRA rearrangement. In addition, 29% of patients with CML had losses on 8q24. In summary, aCGH revealed clonality in eosinophils in most MPNs, suggesting that it could be a useful technique for defining clonality in these diseases. The presence of genetic losses in new regions could provide new insights into the knowledge of these MPN associated with eosinophilia.
authors.affiliation: Department of Hematology, Hospital Clínico Universitario, Valladolid, Spain.
authors.forename: Maryam
authors.initials: M
authors.lastname: Arefi
authors.forename: Cristina
authors.initials: C
authors.lastname: Robledo
authors.forename: María J
authors.initials: MJ
authors.lastname: Peñarrubia
authors.forename: Alfonso
authors.initials: A
authors.lastname: García de Coca
authors.forename: Miguel
authors.initials: M
authors.lastname: Cordero
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Hernández-Rivas
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: García
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11 / Q000737:chemistry; D002888:Chromosomes, Human, Pair 19 / Q000737:chemistry; D002890:Chromosomes, Human, Pair 20 / Q000737:chemistry; D002898:Chromosomes, Human, Pair 8 / Q000737:chemistry; D002908:Chronic Disease; D002999:Clone Cells; D055028:Comparative Genomic Hybridization; D004802:Eosinophilia / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D004804:Eosinophils / Q000378:metabolism* / Q000473:pathology; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D016678:Genome; D042822:Genomic Instability; D019337:Hematologic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics
chemical_list: C581634:BCR-ABL1 fusion protein, human; D020796:Receptor, Platelet-Derived Growth Factor alpha; D016044:Fusion Proteins, bcr-abl
keywords: array comparative genomic hybridization; eosinophilia; myeloproliferative neoplasms