title: Characterization and genotoxicity evaluation of particulate matter collected from industrial atmosphere in Tamil Nadu state, India.
abstract: Ambient particulate matter (PM) collected in the vicinity of five industries (Cement, Chemical, Thermal power plant, Sponge-iron and Steel) in Tamil Nadu state, India was characterized for size distribution, metals and polycyclic aromatic hydrocarbons (PAHs) content. Genotoxicity of PM and organic matter (OM) extracted from PM was measured in human lung cancer cell-line, A549 and in human liver carcinoma cell-line, HepG2, respectively, using the comet assay. PM values varied from 57.0 μg/m(3) of air at Cement industry upstream to 561.0 μg/m(3) of air at Sponge iron industry downstream samples. Their metal content varied from 5.758 μg/m(3) of air at Chemical industry to 46.144 μg/m(3) of air at Sponge iron industry and PAH concentration varied from 0.5 ng/m(3) air in upstream Thermal power plant to 3302.4 ng/m(3) air in downstream Sponge iron industry samples. While all PM samples induced DNA strand breaks at higher dose levels, downstream samples of Steel and Sponge iron industries which contained relatively higher concentrations of PAHs and metals and exhibited higher levels of pro-oxidant activity as measured by DTT activity induced significantly higher levels of DNA damage in HepG2 and A549 cells. Pretreatment of A549 cells with vitamin C or quercetin significantly reduced PM induced DNA strand breaks.
authors.affiliation: Department of Biotechnology, Anna University - BIT Campus, Tiruchirappalli 620024, Tamil Nadu, India. Electronic address: metcitythil.bio@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Senthilkumar
authors.affiliation: Department of Environmental Science, PSG College of Arts and Science, Coimbatore 641014, Tamil Nadu, India. Electronic address: manjuenv88@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Manju
authors.affiliation: Department of Biotechnology, Anna University - BIT Campus, Tiruchirappalli 620024, Tamil Nadu, India. Electronic address: muthu.aut10@gmail.com.
authors.forename: P
authors.initials: P
authors.lastname: Muthuselvam
authors.affiliation: Department of Biotechnology, Anna University - BIT Campus, Tiruchirappalli 620024, Tamil Nadu, India. Electronic address: devishalini87@gmail.com.
authors.forename: D
authors.initials: D
authors.lastname: Shalini
authors.affiliation: Department of Biotechnology, Anna University - BIT Campus, Tiruchirappalli 620024, Tamil Nadu, India. Electronic address: indhumathiveerappan@gmail.com.
authors.forename: V
authors.initials: V
authors.lastname: Indhumathi
authors.affiliation: Department of Environmental Science, PSG College of Arts and Science, Coimbatore 641014, Tamil Nadu, India. Electronic address: kalaiselvi.kannan@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Kalaiselvi
authors.affiliation: Department of Environmental Science, PSG College of Arts and Science, Coimbatore 641014, Tamil Nadu, India. Electronic address: empl2k2@yahoo.co.in.
authors.forename: M
authors.initials: M
authors.lastname: Palanivel
authors.affiliation: Tamil Nadu Pollution Control Board, Salem 635004, Tamil Nadu, India. Electronic address: ppcsekaran@yahoo.com.
authors.forename: P P
authors.initials: PP
authors.lastname: Chandrasekar
authors.affiliation: Department of Biotechnology, Anna University - BIT Campus, Tiruchirappalli 620024, Tamil Nadu, India. Electronic address: rajaguru62@gmail.com.
authors.forename: P
authors.initials: P
authors.lastname: Rajaguru
mesh_terms: D000393:Air Pollutants / Q000032:analysis / Q000737:chemistry / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D004784:Environmental Monitoring; D056945:Hep G2 Cells; D006801:Humans; D007194:India; D007221:Industry; D019216:Metals, Heavy / Q000032:analysis / Q000737:chemistry / Q000633:toxicity; D010316:Particle Size; D052638:Particulate Matter / Q000032:analysis / Q000737:chemistry / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000737:chemistry / Q000633:toxicity; D011794:Quercetin / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology
chemical_list: D000393:Air Pollutants; D000975:Antioxidants; D019216:Metals, Heavy; D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons; D014815:Vitamins; D011794:Quercetin
keywords: Ambient particulate; Chemical composition; DNA damage; Metals; PAH