title: Accurate resistivity mouse brain mapping using microelectrode arrays.
abstract: Electrical impedance spectroscopy measurements were performed in post-mortem mice brains using a flexible probe with an embedded micrometric electrode array. Combined with a peak resistance frequency method this allowed obtaining intrinsic resistivity values of brain tissues and structures with submillimetric resolution. Reproducible resistivity measurements are reported, which allows the resistivity in the cortex, ventricle, fiber tracts, thalamus and basal ganglia to be differentiated. Measurements of brain slices revealed resistivity profiles correlated with the local density of cell bodies hence allowing to discriminate between the different cortical layers. Finally, impedance measurements were performed on a model of cauterized mouse brain evidencing the possibility to measure the spatial extent and the degree of the tissue denaturation due to the cauterization.
authors.affiliation: Microsystems Laboratory, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. Electronic address: amelie.beduer@epfl.ch.
authors.forename: Amélie
authors.initials: A
authors.lastname: Béduer
authors.affiliation: Microsystems Laboratory, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
authors.forename: Pierre
authors.initials: P
authors.lastname: Joris
authors.affiliation: Laboratory of Molecular & Cellular Biology of Alzheimer׳s Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Mosser
authors.affiliation: Laboratory of Neural Microcircuitry, Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
authors.forename: Vincent
authors.initials: V
authors.lastname: Delattre
authors.affiliation: Laboratory of Molecular & Cellular Biology of Alzheimer׳s Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Fraering
authors.affiliation: Microsystems Laboratory, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
authors.forename: Philippe
authors.initials: P
authors.lastname: Renaud
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology* / Q000502:physiology*; D001931:Brain Mapping / Q000295:instrumentation*; D002452:Cell Count / Q000295:instrumentation; D003217:Conductometry / Q000295:instrumentation*; D017097:Electric Impedance; D004867:Equipment Design; D019544:Equipment Failure Analysis; D051379:Mice; D046228:Microarray Analysis / Q000295:instrumentation*; D008839:Microelectrodes; D010992:Plethysmography, Impedance / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: Cortical layers; Impedance spectroscopy; Microelectrodes array; Mouse brain; Neural probe; Thermal cauterization