title: Crystallization and preliminary X-ray analysis of the cardiac transcription factor complex of NKX2.5 and TBX5 with DNA.
abstract: Heart development depends on timely expression of genes regulated by combinatorial interactions of master cardiac transcription factors. To elucidate the molecular basis of their interactions, a ternary complex of cardiac transcription factors, NKX2.5 and TBX5, and their target DNA was studied using X-ray crystallography. Here, the purification, crystallization and preliminary X-ray crystallographic analyses of the NKX2.5 homeodomain and TBX5 DNA-binding domain complex with a DNA element from the -252 promoter region of the atrial natriuretic factor are reported. The crystal diffracted to 2.88 Å resolution and belonged to space group P21, with unit-cell parameters a = 69.30, b = 77.78, c = 77.60 Å, β = 108.31°. Two sets of ternary complexes are present in an asymmetric unit with a solvent content of 54%.
authors.affiliation: Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
authors.forename: Lagnajeet
authors.initials: L
authors.lastname: Pradhan
authors.affiliation: Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
authors.forename: Sunil
authors.initials: S
authors.lastname: Gopal
authors.affiliation: Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Nam
mesh_terms: D000595:Amino Acid Sequence; D009320:Atrial Natriuretic Factor / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites / Q000502:physiology; D003460:Crystallization; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006321:Heart; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D020825:T-Box Domain Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins; C095552:NKX2-5 protein, human; D020825:T-Box Domain Proteins; C103172:T-box transcription factor 5; D014157:Transcription Factors; D009320:Atrial Natriuretic Factor; D004247:DNA
keywords: NKX2.5; TBX5; cardiac transcription factors; target DNA