title: Elevated levels of circulating biomarkers of cell death (nucleosomes) in the patients with plaque psoriasis treated with the Goeckerman regimen.
abstract: BACKGROUND
  Nucleosomes are complexes that are formed during apoptosis. Psoriasis is a chronic skin disease characterized by keratinocyte hyperproliferation and anti-apoptotic features. Presented study was focused to expression of circulating biomarkers of cell death (circulating nucleosomes, CN) during Goeckerman therapy of psoriasis (UV, PAHs).
  
  
  METHODS
  In a group of patients with psoriasis (19), treated with Goeckerman regimen (GR), we evaluated their level of CN, level of chromosomal aberration in peripheral lymphocytes (CA), level of urinary 1-hydroxypyrene (1-OHP) and their value of Psoriasis Area and Severity Index (PASI).
  
  
  RESULTS
  Following the treatment, the serum level of CN and urinary level of 1-OHP (p<0.05) were significantly increased (p<0.01). We found significant correlation between CN and urinary level of 1-OHP after GR (r=0.57; p<0.05). Immediately after the treatment we found significantly increased total numbers of abnormal chromosomes (ABB; p<0.01) and structurally abnormal chromosomes (SAB; p<0.05).
  
  
  CONCLUSIONS
  We found slightly (but statistically significant) elevated level of circulating biomarkers of cell death (nucleosomes) in patients with plaque psoriasis treated with GR (PAHs, UV radiation). We suppose that elevated level of CN is a result of combination of the positive effects of GR and its weak genotoxic effect (mainly PAHs). Conclusions are supported by significant correlation between CN and urinary level of 1-OHP after GR and significantly elevated level of CA after GR (Tab. 2, Fig. 1, Ref. 28).
authors.forename: D
authors.initials: D
authors.lastname: Ranna
authors.forename: C
authors.initials: C
authors.lastname: Andrys
authors.forename: J
authors.initials: J
authors.lastname: Krejsek
authors.forename: K
authors.initials: K
authors.lastname: Hamakova
authors.forename: J
authors.initials: J
authors.lastname: Kremlacek
authors.forename: Z
authors.initials: Z
authors.lastname: Fiala
authors.forename: P
authors.initials: P
authors.lastname: Borsky
authors.forename: L
authors.initials: L
authors.lastname: Borska
mesh_terms: D015415:Biomarkers / Q000097:blood; D016923:Cell Death / Q000502:physiology; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism*; D011565:Psoriasis / Q000097:blood* / Q000628:therapy*; D011721:Pyrenes / Q000652:urine; D014467:Ultraviolet Therapy / Q000379:methods
chemical_list: D015415:Biomarkers; D009707:Nucleosomes; D011721:Pyrenes; C033146:1-hydroxypyrene