title: Functional genetic variants in apoptosis-associated FAS and FASL genes and risk of bladder cancer in a Turkish population.
abstract: BACKGROUND
  The present study aimed to evaluate the role of functional polymorphisms of apoptosis-associated Fatty acid synthase (FAS) and fatty acid synthase ligand (FASL) genes in bladder cancer susceptibility as first presentation in a Turkish population.
  
  
  METHODS
  Genotypes of 91 patients with bladder cancer and 101 healthy controls were evaluated for the polymorphism of FAS-1377 G/A and FASL-844 T/C genes by polymerase chain reaction and restriction fragment length polymorphism analysis.
  
  
  RESULTS
  The frequency of the FAS-1377 G allele was significantly higher in patients with bladder cancer compared to controls (p<0.001). A significantly increased risk for developing bladder cancer was found for the group bearing a T allele for FASL-844 compared to the homozygous FASL-844 CC genotype (p=0.027). FAS-1377 GG genotype and FASL-844 T allele were found to be independently associated with an increased risk of bladder cancer. Additionally, gene-gene interaction analysis revealed that the frequency of FAS-1377AA with FASL-844TC was significantly lower in patients with bladder cancer in comparison to those of controls (p<0.001). Extensive studies for gene-gene interaction are still needed.
  
  
  CONCLUSIONS
  Our study provides new evidence that FAS-1377 G and FASL-844 T alleles may be used as low-penetrant risk factors for bladder cancer development in a Turkish population.
authors.affiliation: Department of Urology, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkey. leventverim@hotmail.com.
authors.forename: Levent
authors.initials: L
authors.lastname: Verim
authors.forename: Ozlem
authors.initials: O
authors.lastname: Timirci-Kahraman
authors.forename: Habib
authors.initials: H
authors.lastname: Akbulut
authors.forename: Alpaslan
authors.initials: A
authors.lastname: Akbas
authors.forename: Tulin
authors.initials: T
authors.lastname: Ozturk
authors.forename: Saime
authors.initials: S
authors.lastname: Turan
authors.forename: Ilhan
authors.initials: I
authors.lastname: Yaylim
authors.forename: Arzu
authors.initials: A
authors.lastname: Ergen
authors.forename: Oguz
authors.initials: O
authors.lastname: Ozturk
authors.forename: Turgay
authors.initials: T
authors.lastname: Isbir
mesh_terms: D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D004843:Epistasis, Genetic; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D012306:Risk; D014421:Turkey; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology; D019014:fas Receptor / Q000235:genetics*
chemical_list: D053222:Fas Ligand Protein; D019014:fas Receptor
keywords: Apoptosis; FAS; FAS ligand; bladder cancer; polymorphism