title: Inhibition of cell proliferation and migration by miR-509-3p that targets CDK2, Rac1, and PIK3C2A.
abstract: CDK2 is a key regulator of cell cycle progression. In this study, we screened for miRNAs targeting CDK2 using a luciferase-3'-untranslated region reporter assay. Among 11 hit miRNAs, miR-509-3p reduced CDK2 protein levels and significantly inhibited cancer cell growth. Microarray, Western blotting, and luciferase reporter analyses revealed additional targets of miR-509-3p, including Rac1 and PIK3C2A. Overexpression of miR-509-3p induced G1 cell-cycle arrest and inhibited colony formation and migration. RNAi experiments indicated that the growth-inhibitory effects of miR-509-3p may occur through down-regulation of CDK2, Rac1, and PIK3C2A. Targeting of multiple growth regulatory genes by miR-509-3p may contribute to effective anti-cancer therapy.
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea.
authors.forename: Sena
authors.initials: S
authors.lastname: Yoon
authors.forename: Eunji
authors.initials: E
authors.lastname: Han
authors.forename: Young-Chul
authors.initials: YC
authors.lastname: Choi
authors.forename: Honghwan
authors.initials: H
authors.lastname: Kee
authors.forename: Yongsu
authors.initials: Y
authors.lastname: Jeong
authors.forename: Jaeseung
authors.initials: J
authors.lastname: Yoon
authors.forename: Kwanghee
authors.initials: K
authors.lastname: Baek
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D051357:Cyclin-Dependent Kinase 2 / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D046228:Microarray Analysis; D009369:Neoplasms / Q000235:genetics / Q000628:therapy*; D014411:Neoplastic Stem Cells; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D019076:Transgenes / Q000235:genetics; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C571982:MIRN509 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D019869:Phosphatidylinositol 3-Kinases; C546621:PIK3C2A protein, human; D051357:Cyclin-Dependent Kinase 2; D020830:rac1 GTP-Binding Protein