title: The oligonucleotide/oligosaccharide-binding fold motif is a poly(ADP-ribose)-binding domain that mediates DNA damage response.
abstract: Oligonucleotide/oligosaccharide-binding (OB) fold is a ssDNA or RNA binding motif in prokaryotes and eukaryotes. Unexpectedly, we found that the OB fold of human ssDNA-binding protein 1 (hSSB1) is a poly(ADP ribose) (PAR) binding domain. hSSB1 exhibits high-affinity binding to PAR and recognizes iso-ADP ribose (ADPR), the linkage between two ADPR units. This interaction between PAR and hSSB1 mediates the early recruitment of hSSB1 to the sites of DNA damage. Mutations in the OB fold of hSSB1 that disrupt PAR binding abolish the relocation of hSSB1 to the sites of DNA damage. Moreover, PAR-mediated recruitment of hSSB1 is important for early DNA damage repair. We have screened other OB folds and found that several other OB folds also recognize PAR. Taken together, our study reveals a PAR-binding domain that mediates DNA damage repair.
authors.affiliation: Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109.
authors.forename: Mo
authors.initials: M
authors.lastname: Li
authors.affiliation: Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109 xiayu@umich.edu.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Yu
mesh_terms: D020816:Amino Acid Motifs; D045744:Cell Line, Tumor; D004249:DNA Damage; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006801:Humans; D024101:Mitochondrial Proteins / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011064:Poly Adenosine Diphosphate Ribose / Q000737:chemistry*; D011485:Protein Binding; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; C543505:INTS3 protein, human; D024101:Mitochondrial Proteins; D009841:Oligonucleotides; D009844:Oligosaccharides; D011994:Recombinant Proteins; C504091:NABP2 protein, human; D011064:Poly Adenosine Diphosphate Ribose