title: Application of magnetic solid phase extraction for separation and determination of aflatoxins B ₁ and B₂ in cereal products by high performance liquid chromatography-fluorescence detection.
abstract: A simple and sensitive method based on the magnetic solid phase extraction with modified magnetic nanoparticles followed by high performance liquid chromatography with fluorescence detection has been developed for extraction and determination of aflatoxins B1 (AFB1) and B2 (AFB2) in cereal products. Magnetic nanoparticle coated with 3-(trimethoxysilyl)-1-propanthiol (TMSPT) and modified with 2-amino-5-mercapto-1,3,4-thiadiazole (AMT) was used as an antibody-free adsorbent. Under the optimal conditions, the calibration curves for AFB1 and AFB2 were linear in the ranges of 0.2-15 μg L(-1) and 0.04-3 μg L(-1), respectively. Detection limit was 0.041 μg L(-1) for AFB1 and 0.013 μg L(-1) for AFB2. The proposed method was successfully applied to the determination of AFB1 and AFB2 in spiked corn and rice samples with an average recovery of 93.5%. The results demonstrated that the developed method is simple, rapid, inexpensive, accurate and remarkably free from interference effects.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, Hamedan, Iran. Electronic address: mhashemi@basu.ac.ir.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Hashemi
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, Hamedan, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Taherimaslak
authors.affiliation: Department of Chemistry, Payam Noor University, Hamedan, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Rashidi
mesh_terms: D000327:Adsorption; D016604:Aflatoxin B1 / Q000032:analysis* / Q000737:chemistry; D000348:Aflatoxins / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013050:Spectrometry, Fluorescence
chemical_list: D000348:Aflatoxins; D058185:Magnetite Nanoparticles; C029753:aflatoxin B2; D016604:Aflatoxin B1
keywords: Aflatoxins B(1) and B(2); Cereals; HPLC-FD; Magnetic solid phase extraction; Modified magnetic nanoparticles