title: Expression of stabilin-1 in M2 macrophages in human granulomatous disease and melanocytic lesions.
abstract: Stabilin-1 is an endocytotic scavenger receptor, specifically expressed by non-continuous sinusoidal endothelial cells in the liver, spleen and lymph nodes and by M2 or alternatively activated macrophages in human malignancies. We analysed paraffin-embedded tissue of melanocytic lesions and granulomatous diseases for stabilin-1 expression, using the human/murine RS1 antibody. The specificity of the RS1 staining was confirmed in a knockout model, as only M2-like tumor-associated macrophages and vessels of a B16F10 melanoma in wild type mice stained positive; while staining of tumor-associated macrophages and vessels originating from stabilin-1 deficient mice remained negative for stabilin-1 specific antibody RS1. In human specimens, the RS1 antibody stained tumor-associated macrophages in all pathological stages of melanoma. In addition, five cases of juvenile xanthogranulomas and one case of necrobiotic xanthogranuloma were strongly stabilin-1 positive, while Th-1 cytokine dominated granulomatous diseases such as sarcoidosis and granulomatous leprosy were negative. Stabilin-1 positive vessels were found in all analysed non-Langerhans cell histiocytoses and melanocytic lesions. No stabilin-1 positive vessels were present in any other granulomatous diseases.
authors.affiliation: Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Schönhaar
authors.affiliation: Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Schledzewski
authors.affiliation: Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Michel
authors.affiliation: Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Dollt
authors.affiliation: Department of Internal Medicine, City Hospital Baden-Baden Balger Str. 50, 76532 Baden-Baden, Germany.
authors.forename: Cleopatra
authors.initials: C
authors.lastname: Gkaniatsou
authors.affiliation: Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany.
authors.forename: Cyrill
authors.initials: C
authors.lastname: Géraud
authors.affiliation: Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Kzhyshkowska
authors.affiliation: Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany.
authors.forename: Sergij
authors.initials: S
authors.lastname: Goerdt
authors.affiliation: Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany.
authors.forename: Astrid
authors.initials: A
authors.lastname: Schmieder
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D064593:Heterografts; D015616:Histiocytosis, Non-Langerhans-Cell / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008545:Melanoma / Q000378:metabolism / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D008875:Middle Aged; D058252:Necrobiotic Xanthogranuloma / Q000378:metabolism* / Q000473:pathology; D009508:Nevus, Pigmented / Q000378:metabolism* / Q000473:pathology; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism*; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology; D014972:Xanthogranuloma, Juvenile / Q000378:metabolism* / Q000473:pathology
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016201:Receptors, Lymphocyte Homing; C449926:STAB1 protein, human; C486732:Stab1 protein, mouse
keywords: M2 macrophages; Melanoma; marker molecules; melanocytic nevus; tumor associated macrophages; tumor classification; xanthogranuloma