title: Comparison of total and IgG ABO antibody titers in healthy individuals by using tube and column agglutination techniques.
abstract: BACKGROUND
  Most immune reactions related to transfusion and transplantation are caused by IgM ABO antibodies. However, IgG also plays an important role in these reactions. Therefore, a method to measure antibodies, including IgG, is necessary. We investigated ABO antibody titers of healthy individuals using a column agglutination technique (CAT) with or without dithiothreitol (DTT) and compared them with titers obtained using a conventional tube method.
  
  
  METHODS
  Among healthy adults who underwent a medical examination, 180 individuals (60 with blood group A, 60 with group B, and 60 with group O) were selected. Antibody titrations were performed using the immediate spin (IS) tube, anti-human globulin (AHG) tube, and CAT with or without DTT methods.
  
  
  RESULTS
  Higher median values of anti-B and anti-A titers in groups A and B individuals, respectively, were obtained using the IS method than using the AHG method. Higher values for group O individuals were obtained using the AHG method. Higher median titers of anti-B and anti-A in group O individuals were obtained using CAT without DTT than using the AHG method. Median titers of anti-B and anti-A in all blood groups were higher in CAT without DTT than in CAT with DTT, especially for group O individuals.
  
  
  CONCLUSIONS
  We recommend CAT with and without DTT for titration of anti-A and anti-B, especially in group O individuals, to provide more sensitive results that include IgG data. Adjustment of insurance coverage of fees associated with antibody titration might be necessary, considering the actual cost of reagents and personnel.
authors.affiliation: Department of Laboratory Medicine, Soonchunhyang University Hospital, Seoul, Korea.
authors.forename: Eun Su
authors.initials: ES
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, Soonchunhyang University Hospital, Seoul, Korea.
authors.forename: Kyung Il
authors.initials: KI
authors.lastname: Jo
authors.affiliation: Department of Laboratory Medicine, Soonchunhyang University Hospital, Seoul, Korea.
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Shin
authors.affiliation: Department of Laboratory Medicine, Soonchunhyang University Hospital, Seoul, Korea.
authors.forename: Rojin
authors.initials: R
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, Soonchunhyang University Hospital, Seoul, Korea.
authors.forename: Tae Yoon
authors.initials: TY
authors.lastname: Choi
authors.affiliation: Department of Laboratory Medicine, Soonchunhyang University Hospital, Seoul, Korea.
authors.forename: Hae In
authors.initials: HI
authors.lastname: Bang
authors.affiliation: Department of Laboratory Medicine, Soonchunhyang University Hospital, Seoul, Korea.
authors.forename: Gum Ran
authors.initials: GR
authors.lastname: Chai
authors.affiliation: Department of Laboratory Medicine, Soonchunhyang University Hospital, Seoul, Korea. ; Department of Laboratory Medicine, Kangwon National University School of Medicine, Chuncheon, Korea.
authors.forename: Soon Gyu
authors.initials: SG
authors.lastname: Yun
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000328:Adult; D000372:Agglutination Tests / Q000295:instrumentation; D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008875:Middle Aged
chemical_list: D000017:ABO Blood-Group System; D000906:Antibodies; D007074:Immunoglobulin G
keywords: ABO antibody; Dithiothreitol; IgG; Titer