title: The use of fluorescent proteins to analyze circadian rhythms.
abstract: Compared with luciferase which is widely used as a reporter for circadian rhythms in Arabidopsis thaliana, available fluorescent markers are generally too stable to allow circadian oscillations to be measured. However, we have developed a technique to use the nuclear localization of circadian-controlled transcription factors fused to a fluorescent reporter as a means of measuring circadian rhythms. This technique has the advantage of being suitable for analyzing rhythms at the level of individual cells and in living plants.
authors.affiliation: Department of Plant and Environmental Sciences, Hebrew University, Edmund Safra Givat Ram Campus, Jerusalem, 91904, Israel.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Shor
authors.forename: Miriam
authors.initials: M
authors.lastname: Hassidim
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Green
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D057906:Circadian Clocks / Q000235:genetics*; D002940:Circadian Rhythm / Q000235:genetics*; D015870:Gene Expression; D017930:Genes, Reporter; D018613:Microscopy, Confocal; D030821:Plants, Genetically Modified