title: Magnetic separation techniques in sample preparation for biological analysis: a review.
abstract: Sample preparation is a fundamental and essential step in almost all the analytical procedures, especially for the analysis of complex samples like biological and environmental samples. In past decades, with advantages of superparamagnetic property, good biocompatibility and high binding capacity, functionalized magnetic materials have been widely applied in various processes of sample preparation for biological analysis. In this paper, the recent advancements of magnetic separation techniques based on magnetic materials in the field of sample preparation for biological analysis were reviewed. The strategy of magnetic separation techniques was summarized. The synthesis, stabilization and bio-functionalization of magnetic nanoparticles were reviewed in detail. Characterization of magnetic materials was also summarized. Moreover, the applications of magnetic separation techniques for the enrichment of protein, nucleic acid, cell, bioactive compound and immobilization of enzyme were described. Finally, the existed problems and possible trends of magnetic separation techniques for biological analysis in the future were proposed.
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Jincan
authors.initials: J
authors.lastname: He
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Huang
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: zzm@mail.sysu.edu.cn.
authors.forename: Zhuomin
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: cesgkl@mail.sysu.edu.cn.
authors.forename: Gongke
authors.initials: G
authors.lastname: Li
mesh_terms: D001681:Biological Assay / Q000379:methods*; D060328:Magnetic Phenomena; D008280:Magnetics / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D013048:Specimen Handling / Q000379:methods*
keywords: Bioactive compounds; Biological analysis; Biological macromolecules; Magnetic separation; Sample preparation