title: The protective effect of quercetin on IMA levels and apoptosis in experimental ovarian ischemia-reperfusion injury.
abstract: OBJECTIVE
  To investigate the protective effect of quercetin (QE), an anti-inflammatory and anti-oxidant agent, on torsion-detorsion induced histopathological changes and blood IMA levels in experimental ovarian ischemia-reperfusion (IR) injury.
  
  
  METHODS
  Twenty-four female Wistar rats were randomly divided into four groups in this study (n=6). Group I, (sham operation); Group II, torsion-detorsion plus saline (IR); Group III, torsion-detorsion plus solvent (dimethylsulfoxide: DMSO, IR+DMSO); Group IV, torsion-detorsion plus 15 mg/kg/bw quercetin (IR+QE) injected intraperitoneally 30 min prior to detorsion. After 3h of reperfusion, the right ovaries were removed surgically. The ovary tissue samples were fixed in 10% formalin solution for histopathological and immunohistochemical examination. Blood samples were obtained at the end of the procedures for each group of animals.
  
  
  RESULTS
  Ovarian sections in Groups II and III showed higher follicular cell degeneration, hemorrhage, vascular congestion and edema when compared with Group I. Administration of quercetin in rats significantly prevented degenerative changes in the ovary. Significantly less histopathological changes were found in Group IV compared with Groups II and III. Caspase-3 and TUNEL positive cells were detected in the ovarian surface, follicle epithelium, and stromal cells in all experimental groups, and there was a significant increase in Groups II and III compared with Group I (P<0.05). Treatment with quercetin decreased the number of caspase-3 and TUNEL positive cells. IR increased the ischemia modified albumin (IMA) levels in comparison to the sham group (1.06 ± 0.10 ABSU and 0.92 ± 0.08 ABSU, P<0.05). Quercetin administration before IR reduced the levels of IMA (0.93 ± 0.08 ABSU, P<0.05).
  
  
  CONCLUSIONS
  Administration of quercetin is effective in preventing tissue damage induced by IR injury in ovaries.
authors.affiliation: Çanakkale Onsekiz Mart University, Medical Faculty, Department of Obstetrics and Gynecology, Çanakkale, Turkey. Electronic address: meruray@yahoo.com.
authors.forename: Meryem
authors.initials: M
authors.lastname: Gencer
authors.affiliation: Trakya University, Medical Faculty, Department of Histology and Embryology, Edirne, Turkey.
authors.forename: Turan
authors.initials: T
authors.lastname: Karaca
authors.affiliation: Çanakkale Onsekiz Mart University, Medical Faculty, Department of Obstetrics and Gynecology, Çanakkale, Turkey.
authors.forename: Ayşe N C
authors.initials: AN
authors.lastname: Güngör
authors.affiliation: Çanakkale Onsekiz Mart University, Medical Faculty, Department of Obstetrics and Gynecology, Çanakkale, Turkey.
authors.forename: Servet Ö
authors.initials: SÖ
authors.lastname: Hacıvelioğlu
authors.affiliation: Trakya University, Medical Faculty, Department of Histology and Embryology, Edirne, Turkey.
authors.forename: Selim
authors.initials: S
authors.lastname: Demirtaş
authors.affiliation: Çanakkale Onsekiz Mart University, Medical Faculty, Department of Biochemistry, Çanakkale, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Turkon
authors.affiliation: Çanakkale Onsekiz Mart University, Medical Faculty, Department of Obstetrics and Gynecology, Çanakkale, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Uysal
authors.affiliation: Beykoz Government Hospital, Department of Obstetrics and Gynecology, İstanbul, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Korkmaz
authors.affiliation: Çanakkale Onsekiz Mart University, Medical Faculty, Department of Obstetrics and Gynecology, Çanakkale, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Coşar
authors.affiliation: Dokuz Eylül University, Medical Faculty, Department of Anestesiology, İzmir, Turkey.
authors.forename: Volkan
authors.initials: V
authors.lastname: Hancı
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D017209:Apoptosis; D015415:Biomarkers / Q000097:blood; D053148:Caspase 3 / Q000032:analysis; D005260:Female; D010049:Ovarian Diseases / Q000150:complications*; D010053:Ovary / Q000737:chemistry; D011794:Quercetin / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D012709:Serum Albumin; D000075462:Serum Albumin, Human; D014102:Torsion Abnormality / Q000150:complications*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D012709:Serum Albumin; C561522:ischemia-modified albumin; D011794:Quercetin; D053148:Caspase 3; D000075462:Serum Albumin, Human
keywords: Apoptosis; Caspase-3; Ischemia modified albumin; Ovarian torsion; Quercetin; TUNEL