title: Acid-sensing ion channels in mouse olfactory bulb M/T neurons.
abstract: The olfactory bulb contains the first synaptic relay in the olfactory pathway, the sensory system in which odorants are detected enabling these chemical stimuli to be transformed into electrical signals and, ultimately, the perception of odor. Acid-sensing ion channels (ASICs), a family of proton-gated cation channels, are widely expressed in neurons of the central nervous system. However, no direct electrophysiological and pharmacological characterizations of ASICs in olfactory bulb neurons have been described. Using a combination of whole-cell patch-clamp recordings and biochemical and molecular biological analyses, we demonstrated that functional ASICs exist in mouse olfactory bulb mitral/tufted (M/T) neurons and mainly consist of homomeric ASIC1a and heteromeric ASIC1a/2a channels. ASIC activation depolarized cultured M/T neurons and increased their intracellular calcium concentration. Thus, ASIC activation may play an important role in normal olfactory function.
authors.affiliation: Department of Neurological Surgery, Oregon Health & Science University, Portland, OR 97239.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Li
authors.affiliation: Oregon State University, Corvallis, OR 97331.
authors.forename: Selina Qiuying
authors.initials: SQ
authors.lastname: Liu
authors.affiliation: Department of Neurobiology, Neuroscience Institute, Morehouse School of Medicine, Atlanta, GA 30310.
authors.forename: Koichi
authors.initials: K
authors.lastname: Inoue
authors.affiliation: Legacy Research, Portland, OR 97234.
authors.forename: Jinquan
authors.initials: J
authors.lastname: Lan
authors.affiliation: Department of Neurobiology, Neuroscience Institute, Morehouse School of Medicine, Atlanta, GA 30310.
authors.forename: Roger P
authors.initials: RP
authors.lastname: Simon
authors.affiliation: Department of Neurobiology, Neuroscience Institute, Morehouse School of Medicine, Atlanta, GA 30310 zxiong@msm.edu.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Xiong
mesh_terms: D062565:Acid Sensing Ion Channels / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology; D002478:Cells, Cultured; D015640:Ion Channel Gating / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology*; D051379:Mice; D009830:Olfactory Bulb / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000145:classification / Q000502:physiology*
chemical_list: D062565:Acid Sensing Ion Channels; D002118:Calcium