title: Applying accelerator mass spectrometry for low-level detection of complex engineered nanoparticles in biological media.
abstract: Complex engineered nanoparticles (CENPs), which have different core and surface components, are being developed for medicinal, pharmaceutical and industrial applications. One of the key challenges for environmental health and safety assessments of CENPs is to identify and quantity their transformations in biological environments. This study reports the effects of in vivo exposure of citrate-coated nanoalumina with different rare isotope labels on each component. This CENP was dosed to the rat and accelerator mass spectrometry (AMS) was used to quantify (26)Al, (14)C, and their ratio in the dosing material and tissue samples. For CENPs detected in the liver, the rare isotope ratio, (14)C/(26)Al, was 87% of the dosing material's ratio. The citrate coating on the nanoalumina in the liver was stable or, if it degraded, its metabolites were incorporated with nearby tissues. However, in brain and bone where little alumina was detected, the rare isotope ratio greatly exceeded that of the dosing material. Therefore, in the animal, citrate dissociated from CENPs and redistributed to brain and bone. Tracking both the core and surface components by AMS presents a new approach for characterizing transformations of CENPs components in biological milieu or environments.
authors.affiliation: Departments of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506, United States.
authors.forename: Binghui
authors.initials: B
authors.lastname: Wang
authors.affiliation: Purdue Rare Isotope Measurement Laboratory, Department of Physics, Purdue University, West Lafayette, IN 47907, United States.
authors.forename: George S
authors.initials: GS
authors.lastname: Jackson
authors.affiliation: Department of Pharmaceutical Sciences, University of Kentucky, Lexington, KY 40536, United States.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Yokel
authors.affiliation: Departments of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506, United States. Electronic address: eric.grulke@uky.edu.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Grulke
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D019343:Citric Acid / Q000737:chemistry; D008099:Liver / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D053768:Metal Nanoparticles / Q000032:analysis* / Q000737:chemistry*; D010316:Particle Size; D011868:Radioisotopes; D051381:Rats
chemical_list: D011868:Radioisotopes; D019343:Citric Acid
keywords: Accelerator mass spectrometry; Biodistribution; Biotransformation; Complex engineered nanoparticles; In vivo exposure; Nanoalumina