title: Sensitive and selective spectrophotometric assay of piroxicam in pure form, capsule and human blood serum samples via ion-pair complex formation.
abstract: A simple, accurate and highly sensitive spectrophotometric method has been developed for the rapid determination of piroxicam (PX) in pure and pharmaceutical formulations. The proposed method involves formation of stable yellow colored ion-pair complexes of the amino derivative (basic nitrogen) of PX with three sulphonphthalein acid dyes namely; bromocresol green (BCG), bromothymol blue (BTB), bromophenol blue (BPB) in acidic medium. The colored species exhibited absorption maxima at 438, 429 and 432 nm with molar absorptivity values of 9.400×10(3), 1.218×10(3) and 1.02×10(4) L mol(-1) cm(-1) for PX-BCG, PX-BTB and PX-BPB complexes, respectively. The effect of optimum conditions via acidity, reagent concentration, time and solvent were studied. The reactions were extremely rapid at room temperature and the absorbance values remained constant for 48h. Beer's law was obeyed with a good correlation coefficient in the concentration ranges 1-100 μg mL(-1) for BCG, BTB complexes and 1-95 μg mL(-1) for BPB complex. The composition ratio of the ion-pair complexes were found to be 1:1 in all cases as established by Job's method. No interference was observed from common additives and excipients which may be present in the pharmaceutical preparations. The proposed method was successfully applied for the determination of PX in capsule and human blood serum samples with good accuracy and precision.
authors.affiliation: Department of Chemistry, Faculty of Science, University of Guilan, Namjoo Street, P.B. 41335-1914, Rasht, Iran. Electronic address: nializadeh@yahoo.com.
authors.forename: Nina
authors.initials: N
authors.lastname: Alizadeh
authors.affiliation: Department of Chemistry, Faculty of Science, University of Guilan, Namjoo Street, P.B. 41335-1914, Rasht, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Keyhanian
mesh_terms: D001961:Bromcresol Green / Q000737:chemistry; D001978:Bromphenol Blue / Q000737:chemistry; D001979:Bromthymol Blue / Q000737:chemistry; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D004396:Coloring Agents / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D010894:Piroxicam / Q000737:chemistry*; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry*; D012997:Solvents; D013053:Spectrophotometry; D013455:Sulfur / Q000737:chemistry; D013696:Temperature
chemical_list: D004396:Coloring Agents; D007202:Indicators and Reagents; D012997:Solvents; D001978:Bromphenol Blue; D010894:Piroxicam; D013455:Sulfur; D001961:Bromcresol Green; D001979:Bromthymol Blue
keywords: Bromocresol green; Bromophenol blue; Bromothymol blue; Piroxicam; Spectrophotometry