title: Analysis of protein structure by cross-linking combined with mass spectrometry.
abstract: Cross-linking combined with mass spectrometry is a powerful technique to study protein structure. Here, we present an optimized protocol for the preparation, processing, and analysis of a protein sample cross-linked with isotopically coded, affinity-enrichable, and CID-cleavable cross-linker CyanurBiotinDimercaptoPropionylSuccinimide using LC/ESI-MS/MS on a Thermo Scientific Orbitrap mass spectrometer.
authors.affiliation: University of Victoria - Genome BC Proteomics Centre, University of Victoria, 3101-4464 Markham St, Victoria, BC, 78Z 7X8, Canada.
authors.forename: Evgeniy V
authors.initials: EV
authors.lastname: Petrotchenko
authors.forename: Karl A T
authors.initials: KA
authors.lastname: Makepeace
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Serpa
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Borchers
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003432:Cross-Linking Reagents; D011506:Proteins