title: Induction of dedifferentiated male mouse adipose stromal vascular fraction cells to primordial germ cell-like cells.
abstract: The adipose stromal vascular fraction (SVF) contains abundant mesenchymal stem cell populations that have a limited ability to self-renew and differentiate. Male mouse adipose SVF cells were dedifferentiated by reprogramming factors (c-Myc, Oct4, Sox2, and Klf4) to form embryonic stem cell-like cells (ESCLCs), which upgraded their limited differentiation potential. The ESCLCs were induced to differentiate toward epiblast-like cells (EpiLCs) and primordial germ cell-like cells (PGCLCs) by culturing in media supplied with activin A and BMP-4, respectively. The derived ESCLCs possess embryonic stem cell features and can automatically form embryonic bodies. After culture in EpiLC induction medium for 2-3 days, ESCLCs formed flattened epithelial structures that were different from their original water drop-like colonies, and the expression of pluripotency-related genes decreased. When the cells that had been cultured in EpiLC induction medium for 2 days were isolated and cultured in PGCLC induction medium for 4-6 days, they formed typical water drop-like colonies again. Moreover, expression of the pluripotency-related genes and the primordial germ cell (PGC) specification-related genes increased. During progression from ESCLCs toward EpiLCs and PGCLCs, the levels of histone methylases H3K9me2 and H3K27me3 kept changing, which resembled those seen in PGC specification. The derived PGCLCs expressed SSEA-1, Blimp-1, and Stella. Furthermore, methylation of Igf2r and Snrpn was retained, but H19 and Kcnq1ot1 methylation levels were slightly reduced compared to non-PGCLCs, suggesting that the derived PGCLCs may have initiated the process of imprint erasure.
authors.affiliation: Guangdong Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Medical Center of Peking University and Hong Kong Science and Technology University, Guangdong, 518036, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Cui
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Qi
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xia
authors.initials: X
authors.lastname: Long
authors.forename: Jie
authors.initials: J
authors.lastname: Qin
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D054337:Cell Dedifferentiation / Q000187:drug effects; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000187:drug effects; D002868:Chromosomal Proteins, Non-Histone; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005854:Germ Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism; D000090062:Kruppel-Like Factor 4; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000074462:Positive Regulatory Domain I-Binding Factor 1; D012097:Repressor Proteins / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology*; D014157:Transcription Factors / Q000378:metabolism / Q000494:pharmacology
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C462180:Dppa3 protein, mouse; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; C513258:Prdm1 protein, mouse; D012097:Repressor Proteins; D014157:Transcription Factors; D000076983:Histone Methyltransferases; D000074462:Positive Regulatory Domain I-Binding Factor 1; D011495:Histone-Lysine N-Methyltransferase
keywords: dedifferentiation; embryonic stem cell; epiblast cell; imprint erasure; primordial germ cell; stromal vascular fraction cell