title: A novel low molecular weight endo-xylanase from Streptomyces sp. CS628 cultivated in wheat bran.
abstract: An extracellular low molecular weight xylanase (Xyn628) from Streptomyces sp. CS628 was isolated from Korean soil sample, produced in wheat bran medium, purified, and biochemically characterized. Xyn628 was purified 4.8-fold with a 33.78 % yield using Sepharose CL-6B column chromatography. The purified xylanase was ~18.1 kDa estimated by SDS-PAGE and xylan zymography. N-terminal amino acid sequences of Xyn628 were AYIKEVVSRAYM. The enzyme was found to be stable in a broad range of pH (5.0-13.0) and up to 60 °C and have optimal pH and temperature of pH 11.0 and 60 °C, respectively. Xyn628 activities were remarkable affected by various detergents, chelators, modulators, and metal ions. The xylanase produced xylobiose and xylotriose as principal hydrolyzed end products from the xylan. It was found to degrade agro-waste materials like corn cob and wheat bran by Xyn628 (20 U/g) as shown by electron microscopy. As being simple in purification, low molecular weight, alkaline, thermostable, and ability to produce xylooligosaccharides show that Xyn628 has potential applications in bioindustries as a biobleaching agent or/and xylooligosaccharides production with an appropriate utilization of agro-waste.
authors.affiliation: Department of Pharmacy, Chosun University, Gwangju, 501-759, Korea.
authors.forename: Md Arifur
authors.initials: MA
authors.lastname: Rahman
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Choi
authors.forename: G C
authors.initials: GC
authors.lastname: Pradeep
authors.forename: Yoon Seok
authors.initials: YS
authors.lastname: Choi
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Choi
authors.forename: Seung Sik
authors.initials: SS
authors.lastname: Cho
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Yoo
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001673:Biodegradation, Environmental; D018533:Biomass; D004043:Dietary Fiber; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000235:genetics; D004795:Enzyme Stability; D005965:Glucuronates / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009844:Oligosaccharides / Q000096:biosynthesis; D012988:Soil Microbiology; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D004043:Dietary Fiber; D005965:Glucuronates; D009844:Oligosaccharides; C570991:xylooligosaccharide; D043364:Endo-1,4-beta Xylanases