title: Platelet-derived growth factor receptor beta: a novel urinary biomarker for recurrence of non-muscle-invasive bladder cancer.
abstract: Non-muscle-invasive bladder cancer (NMIBC) is one of the most common malignant tumors in the urological system with a high risk of recurrence, and effective non-invasive biomarkers for NMIBC relapse are still needed. The human urinary proteome can reflect the status of the microenvironment of the urinary system and is an ideal source for clinical diagnosis of urinary system diseases. Our previous work used proteomics to identify 1643 high-confidence urinary proteins in the urine from a healthy population. Here, we used bioinformatics to construct a cancer-associated protein-protein interaction (PPI) network comprising 16 high-abundance urinary proteins based on the urinary proteome database. As a result, platelet-derived growth factor receptor beta (PDGFRB) was selected for further validation as a candidate biomarker for NMIBC diagnosis and prognosis. Although the levels of urinary PDGFRB showed no significant difference between patients pre- and post-surgery (n = 185, P>0.05), over 3 years of follow-up, urinary PDGFRB was shown to be significantly higher in relapsed patients (n = 68) than in relapse-free patients (n = 117, P<0.001). The levels of urinary PDGFRB were significantly correlated with the risk of 3-year recurrence of NMIBC, and these levels improved the accuracy of a NMIBC recurrence risk prediction model that included age, tumor size, and tumor number (area under the curve, 0.862; 95% CI, 0.809 to 0.914) compared to PDGFR alone. Therefore, we surmise that urinary PDGFRB could serve as a non-invasive biomarker for predicting NMIBC recurrence.
authors.affiliation: Department of Urology, Xinqiao Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Feng
authors.affiliation: Chongqing Key Laboratory for Disease Proteomics; State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Urology, Xinqiao Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Song
authors.affiliation: Chongqing Key Laboratory for Disease Proteomics; State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Simpson
authors.affiliation: Chongqing Key Laboratory for Disease Proteomics; State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Chongqing Key Laboratory for Disease Proteomics; State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Gaoxing
authors.initials: G
authors.lastname: Luo
authors.affiliation: Chongqing Key Laboratory for Disease Proteomics; State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Urology, Xinqiao Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Chibing
authors.initials: C
authors.lastname: Huang
mesh_terms: D000367:Age Factors; D000368:Aged; D019540:Area Under Curve; D015415:Biomarkers / Q000652:urine; D019295:Computational Biology; D016208:Databases, Factual; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D011379:Prognosis; D060066:Protein Interaction Maps; D012372:ROC Curve; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism / Q000652:urine*; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery
chemical_list: D015415:Biomarkers; D020797:Receptor, Platelet-Derived Growth Factor beta