title: DESI-MS as a tool for direct lipid analysis in cultured cells.
abstract: Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface preparation of cell culture slides, induction of oxidative stress, and cell monolayer culture preparation as well as optimization of the analysis. Advantages and drawbacks of the method were also discussed.
authors.affiliation: AGH University of Science and Technology, Kraków, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Bodzon-Kulakowska
authors.affiliation: AGH University of Science and Technology, Kraków, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Cichon
authors.affiliation: AGH University of Science and Technology, Kraków, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Golec
authors.affiliation: AGH University of Science and Technology, Kraków, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Drabik
authors.affiliation: AGH University of Science and Technology, Kraków, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Ner
authors.affiliation: AGH University of Science and Technology, Kraków, Poland. piotr.suder@agh.edu.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Suder
keywords: Cell culture; Desorption electrospray ionization (DESI); Lipid analysis; Oxidative stress