title: Generation and application of a 293 cell line stably expressing bovine interferon-gamma.
abstract: A stable mammalian cell line expressing highly active bovine interferon-gamma (BoIFN-γ) was generated using Flp recombinase-mediated integration. This recombinant 293 cell line (B1) efficiently secreted FLAG-tagged BoIFN-γ protein into the culture supernatant, as determined by ELISA and Western blot. The recombinant BoIFN-γ exhibited high anti-viral activity, suggesting that the 293 cells expressed BoIFN-γ that structurally and biologically resembled the natural protein. Two monoclonal antibodies (mAbs) with high affinity for the 293 cell-expressed BoIFN-γ were identified using this cell line, and these mAbs can be used for the development of diagnostic kits. Thus, this work demonstrates the successful generation of a 293 cell line that produces large quantities of highly active BoIFN-γ and demonstrates its potential application in the research of bovine infectious diseases.
authors.affiliation: Jiangsu Key Lab of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Zhengzhong
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Jiangsu Key Lab of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Fengli
authors.initials: F
authors.lastname: Shan
authors.affiliation: Jiangsu Key Lab of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Fa
authors.initials: F
authors.lastname: Shan
authors.affiliation: Jiangsu Key Lab of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Meng
authors.affiliation: Department of Pathobiology and Veterinary Sciences, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Unit of Innate Defense and Immune Modulation, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Lab of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. Electronic address: chenxiang@yzu.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Jiangsu Key Lab of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. Electronic address: jiao@yzu.edu.cn.
authors.forename: Xinan
authors.initials: X
authors.lastname: Jiao
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D019556:COS Cells; D002417:Cattle; D002460:Cell Line; D004254:DNA Nucleotidyltransferases; D057809:HEK293 Cells; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D011994:Recombinant Proteins; D007371:Interferon-gamma; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase
keywords: 293 cell line; Antibody; Bovine interferon-gamma