title: In ovo carbohydrate supplementation modulates growth and immunity-related genes in broiler chickens.
abstract: A study was undertaken to investigate the role of in ovo administrated carbohydrates on the expression pattern of growth and immune-related genes. In ovo injections (n = 400) were carried out on the 14th day of incubation into the yolk sac/amnion of the broiler chicken embryos. Expression of growth-related genes: chicken growth hormone (cGH), insulin-like growth factor-I & II (IGF-I & II) and mucin were studied in hepatic and jejunum tissues of late-term embryo and early post-hatch chicks. Expression of candidate immune genes: Interleukin-2, 6, 10 and 12 (IL-2, IL-6, IL-10 and IL-12), Tumour necrosis factor-alpha (TNF-α) and Interferon gamma (IFN-γ) were studied in peripheral blood monocyte cells of in ovo-injected and control birds following antigenic stimulation with sheep RBC (SRBC) or mitogen concanavalin A (Con-A). Glucose injection significantly increased the expression of IGF-II gene during embryonic period and both cGH and IGF-II in early post-hatch period, while ribose-injected chicks had higher expression of IGF-II gene during embryonic stage. Enhanced mucin gene expression was also observed in fructose-injected chicks during embryonic age. Glucose-injected chicks had higher expression of IL-6 or IL-10, while those injected with fructose or ribose had higher expression of IL-2, IL-12 and IFN gamma. It is concluded that in ovo supplementation of carbohydrates might help in improving the growth of late-term embryos and chicks. In ovo glucose could modulate humoral-related immunity, while fructose or ribose might help in improving the cellular immunity in broiler chickens.
authors.affiliation: Central Avian Research Institute, Izatnagar, UP, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Bhanja
authors.affiliation: Central Avian Research Institute, Izatnagar, UP, India.
authors.forename: A
authors.initials: A
authors.lastname: Goel
authors.affiliation: Central Avian Research Institute, Izatnagar, UP, India.
authors.forename: N
authors.initials: N
authors.lastname: Pandey
authors.affiliation: Central Avian Research Institute, Izatnagar, UP, India.
authors.forename: M
authors.initials: M
authors.lastname: Mehra
authors.affiliation: Central Avian Research Institute, Izatnagar, UP, India.
authors.forename: S
authors.initials: S
authors.lastname: Majumdar
authors.affiliation: Central Avian Research Institute, Izatnagar, UP, India.
authors.forename: A B
authors.initials: AB
authors.lastname: Mandal
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000008:administration & dosage / Q000494:pharmacology*; D002642:Chick Embryo; D002645:Chickens / Q000254:growth & development* / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000276:immunology; D010063:Ovum
chemical_list: D002241:Carbohydrates
keywords: broiler chickens; carbohydrates; gene expression; growth and immunity; in ovo injection