title: An innovative approach to treating dental decay in children. A new anti-caries agent.
abstract: The aim of this work is to evaluate the antimicrobial and cytotoxic activity of a formulation containing silver nanoparticles and chitosan, provisionally called nano silver fluoride (NSF), against Streptococcus mutans in comparison to chlorhexidine and silver diamine fluoride (SDF). The product was characterised by transmission electron microscopy and UV-Vis absorption spectroscopy. The minimum inhibition concentration (MIC) was evaluated by the spectrophotometric microdilution method and turbidity. The minimum bactericide concentration (MBC) was evaluated in brain heart infusion plates, and cytotoxicity was evaluated by haemolytic activity. The MIC and MBC for NSF were, respectively, 33.54 ± 14.52 and 50.32 µg/mL; for SDF were 33.33 ± 14.43 and 50.0 µg/mL, respectively; and for CHX were 3.3 ± 0.5 and 6 µg/mL, respectively. An ANOVA for MIC gave P = 0.032, and for MBC P = 0.035. The cytotoxic effect of NSF compared to SDF demonstrated a statistically significant difference in the MIC value (t test P < 0.05). The NSF formulation may be effective against S. mutans with much lower doses, may have lower toxicity than SDF, and may not stain teeth.
authors.affiliation: Department of Paediatric Dentistry, Faculty of Dentistry, University of Pernambuco, Av: Gal. Newton Cavalcanti nº 1650, Camaragibe, PE, CEP 54 753-020, Brazil, andreadoutorado@gmail.com.
authors.forename: Andréa Gadelha Ribeiro
authors.initials: AG
authors.lastname: Targino
authors.forename: Miguel Angel Pelagio
authors.initials: MA
authors.lastname: Flores
authors.forename: Valdeci Elias
authors.initials: VE
authors.lastname: dos Santos Junior
authors.forename: Fabiana
authors.initials: F
authors.lastname: de Godoy Bené Bezerra
authors.forename: Hilzeth
authors.initials: H
authors.lastname: de Luna Freire
authors.forename: André
authors.initials: A
authors.lastname: Galembeck
authors.forename: Aronita
authors.initials: A
authors.lastname: Rosenblatt
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002648:Child; D003731:Dental Caries / Q000188:drug therapy*; D006801:Humans; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D013056:Spectrophotometry, Ultraviolet; D013295:Streptococcus mutans / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents