title: Cloning, expression, crystallization and preliminary X-ray diffraction studies of staphylococcal superantigen-like protein 1 (SSL1).
abstract: Staphylococcus aureus produces a family of exotoxins which are structural homologues of superantigens and thus are called staphylococcal superantigen-like proteins (SSLs). Amongst the 14 SSL genes, ssl1 (SAOUHSC_00383) has been cloned in the pQE30 expression vector, overexpressed in Escherichia coli M15 (pREP4) cells and the protein purified to homogeneity. The protein was crystallized using 6% Tacsimate pH 6.0, 0.1 M MES pH 6.0, 25%(w/v) polyethylene glycol 3350, 100 mM NDSB 256 at 298 K by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 77.9, b = 70.5, c = 126.5 Å, β = 106.2°. X-ray diffraction data were collected and processed to a maximum resolution of 2.5 Å. The crystal contains six molecules in the asymmetric unit.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721 302, India.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Dutta
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721 302, India.
authors.forename: Anirudha
authors.initials: A
authors.lastname: Dutta
authors.affiliation: Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong 793 022, India.
authors.forename: Atanu
authors.initials: A
authors.lastname: Bhattacharjee
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721 302, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Basak
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721 302, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Das
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D003460:Crystallization; D005098:Exotoxins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D013211:Staphylococcus aureus / Q000235:genetics; D018089:Superantigens / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D005098:Exotoxins; D018089:Superantigens
keywords: SSL1; Staphylococcus aureus; superantigen-like proteins