title: [Porphyromonas gingivalis infection enhanced the adhesion of monocytes to endothelial].
abstract: OBJECTIVE
  To observe if Porphyromonas gingivalis (Pg) infection could enhance the adhesion of human monocytic cell line (THP-1) to human umbilical vein endothelial cells (HUVEC).
  
  
  METHODS
  PgATCC33277 was cultured in anaerobic jar, and THP-1 was infected with various concentrations of PgATCC33277 at multiplicity of infection (MOI) of 1: 100 for 8 and 24 hours, respectively. After removal of the free Pg, THP-1 cells were cocultured with HUVEC for 1 hour to observe the adhesion of THP-1 to HUVEC.HUVEC with adhesive THP-1 cells were co-cultured for additional 23 hours. The medium and cells were separately collected. The expression of related chemotactic cytokine[monocyte chemotactic protein 1(MCP-1) and interleukin 8(IL-8)] and intercellular adhesion molecule-1(ICAM-1) were detected with enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The adhesion of THP-1 to HUVEC was enhanced (13.8%-35.2%, P = 0.006) and the expression of ICAM-1 of HUVEC was increased from (132.5 ± 7.7) to (164.9 ± 9.1) ng/L (P = 0.005) after infection for 24 hours by Pg. Both of the secreted MCP-1 and IL-8 elevated after infection of Pg for 24 hours from (183.2 ± 3.1) to (221.0 ± 4.9) ng/L (P = 0.012) and from (587.2 ± 5.1) to (787.2 ± 10.3) ng/L (P = 0.002), respectively.
  
  
  CONCLUSIONS
  Pg could enhance the adhesion of monocytes to endothelial cells and stimulate the inflammation, suggesting that Pg infection may be one of the risk factors in promoting the development of atherosclerosis.
authors.affiliation: Department of Cell Biology and Genetics, Medical School of Nanjing University, Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology and Genetics, Medical School of Nanjing University, Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Cell Biology and Genetics, Medical School of Nanjing University, Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Cell Biology and Genetics, Medical School of Nanjing University, Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Cell Biology and Genetics, Medical School of Nanjing University, Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China.
authors.forename: Weibin
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Cell Biology and Genetics, Medical School of Nanjing University, Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China. Email: likuanyu@nju.edu.cn.
authors.forename: Kuanyu
authors.initials: K
authors.lastname: Li
mesh_terms: D016866:Bacteroidaceae Infections / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D018920:Coculture Techniques; D042783:Endothelial Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D009000:Monocytes; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*; D014471:Umbilical Veins
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D016209:Interleukin-8; D018799:Intercellular Adhesion Molecule-1