title: CRISPR transcriptional repression devices and layered circuits in mammalian cells.
abstract: A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.
authors.affiliation: 1] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Samira
authors.initials: S
authors.lastname: Kiani
authors.affiliation: Raytheon BBN Technologies, Cambridge, Massachusetts, USA.
authors.forename: Jacob
authors.identifier: 0000000216635102
authors.initials: J
authors.lastname: Beal
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Ebrahimkhani
authors.affiliation: 1] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3] Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Huh
authors.affiliation: 1] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Richard N
authors.initials: RN
authors.lastname: Hall
authors.affiliation: 1] Bioinformatics Division, Center for Synthetic & Systems Biology, Tsinghua National Laboratory for Information Science and Technology, Beijing, China. [2] Department of Automation, Tsinghua University, Beijing, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Yinqing
authors.initials: Y
authors.lastname: Li
authors.affiliation: 1] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3] Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Ron
authors.initials: R
authors.lastname: Weiss
mesh_terms: D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D005786:Gene Expression Regulation; D018390:Gene Targeting; D005818:Genetic Engineering / Q000295:instrumentation* / Q000379:methods; D057809:HEK293 Cells; D006801:Humans; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics; D012320:RNA Polymerase III / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D058727:RNA, Small Untranslated
chemical_list: D012319:RNA Polymerase II; D012320:RNA Polymerase III; D058727:RNA, Small Untranslated