title: Superhydrophobic nitric oxide-releasing xerogels.
abstract: Superhydrophobic nitric oxide (NO)-releasing xerogels were prepared by spray-coating a fluorinated silane/silica composite onto N-diazeniumdiolate NO donor-modified xerogels. The thickness of the superhydrophobic layer was used to extend NO release durations from 59 to 105h. The resulting xerogels were stable, maintaining superhydrophobicity for up to 1month (the longest duration tested) when immersed in solution, with no leaching of silica or undesirable fragmentation detected. The combination of superhydrophobicity and NO release reduced viable Pseudomonas aeruginosa adhesion by >2-logs. The killing effect of NO was demonstrated at longer bacterial contact times, with superhydrophobic NO-releasing xerogels resulting in 3.8-log reductions in adhered viable bacteria vs. controls. With no observed toxicity to L929 murine fibroblasts, NO-releasing superhydrophobic membranes may be valuable antibacterial coatings for implants as they both reduce adhesion and kill bacteria that do adhere.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, CB#3290, Chapel Hill, NC 27599, USA.
authors.forename: Wesley L
authors.initials: WL
authors.lastname: Storm
authors.affiliation: Department of Chemistry, Kwangwoon University, Seoul 139-701, South Korea.
authors.forename: Jonghae
authors.initials: J
authors.lastname: Youn
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, CB#3290, Chapel Hill, NC 27599, USA.
authors.forename: Katelyn P
authors.initials: KP
authors.lastname: Reighard
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, CB#3290, Chapel Hill, NC 27599, USA.
authors.forename: Brittany V
authors.initials: BV
authors.lastname: Worley
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, CB#3290, Chapel Hill, NC 27599, USA.
authors.forename: Hetali M
authors.initials: HM
authors.lastname: Lodaya
authors.affiliation: Department of Chemistry, Kwangwoon University, Seoul 139-701, South Korea. Electronic address: jhshin@kw.ac.kr.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, CB#3290, Chapel Hill, NC 27599, USA. Electronic address: schoenfisch@unc.edu.
authors.forename: Mark H
authors.initials: MH
authors.lastname: Schoenfisch
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D004058:Diffusion; D020100:Hydrogels / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry / Q000648:ultrastructure; D009569:Nitric Oxide / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology*; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D053769:Nanocapsules; D012821:Silanes; D009569:Nitric Oxide; D012822:Silicon Dioxide
keywords: Antimicrobial; Nitric oxide; Sol–gel; Superhydrophobic