title: [Effect of RNF2 knockdown on apoptosis and radiosensitivity in glioma U87 cells].
abstract: OBJECTIVE
  To investigate the effect of RING finger protein 2 (RNF2) knockdown on the biological characteristics and radiosensitivity in glioma U87 cells.
  
  
  METHODS
  Plasmids containing shRNA targeting RNF2 were transfected into U87 cells. Real-time quantitative PCR (qRT-PCR) and Western blotting were respectively applied to detect the mRNA and protein level of RNF2. MTT assay was used to detect cell proliferation. Cell cycle and apoptosis were measured by flow cytometry combined with annexin V-FITC/PI staining in the control and RNF2 knockdown cells. Apoptosis was also detected after X-ray radiation.
  
  
  RESULTS
  Both shRNAs efficiently inhibited RNF2 expression in U87 cells. Cell proliferation was obviously depressed in RNF2 knockdown cells. The percentage of cells decreased in S phase (shRNA-NC: 27.31 ± 1.35; shRNF2-1: 16.72 ± 2.90; shRNF2-3: 10.35 ± 1.33) and increased in G1 phase (shRNA-NC: 56.13 ± 1.80; shRNF2-1: 76.32 ± 3.11; shRNF2-3: 80.45 ± 2.83). More cell apoptosis was observed in RNF2 knockdown cells. After X ray radiation, the apoptosis rate was significantly raised in RNF2 knockdown cells (shRNA-NC: 20.88 ± 0.64; shRNF2-1: 39.69 ± 0.57; shRNF2-3: 47.82 ± 0.45).
  
  
  CONCLUSIONS
  Knockdown of RNF2 can inhibit cell proliferation, induce cell cycle arrest and promote apoptosis in U87 cells. RNF2 knockdown can obviously increase the sensitivity of U87 cells to X ray radiation.
authors.affiliation: Department of Neurosurgery, Navy General Hospital, Beijing 100037; Graduate School, Second Military Medical University, Shanghai 200433, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Xi
authors.affiliation: Department of Urology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wei
authors.affiliation: Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Guoxu
authors.initials: G
authors.lastname: Zheng
authors.affiliation: Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Angang
authors.initials: A
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Navy General Hospital, Beijing 100037; Graduate School, Second Military Medical University, Shanghai 200433, China.
authors.forename: Jianning
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Wen
mesh_terms: D017209:Apoptosis / Q000235:genetics* / Q000528:radiation effects*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000235:genetics / Q000528:radiation effects; D005434:Flow Cytometry; D016193:G1 Phase / Q000235:genetics / Q000528:radiation effects; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D005910:Glioma / Q000235:genetics / Q000473:pathology; D006801:Humans; D063150:Polycomb Repressive Complex 1 / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D011836:Radiation Tolerance / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016196:S Phase / Q000235:genetics / Q000528:radiation effects
chemical_list: D063150:Polycomb Repressive Complex 1; C489841:RNF2 protein, human