title: A coumarin-based fluorescent probe for differential identification of sulfide and sulfite in CTAB micelle solution.
abstract: Sulfite and sulfide share several similarities in terms of chemical properties, such as nucleophilic and reducing reactivities. Therefore, they may disturb the detection of each other. In order to discriminate between these two kinds of sulfur-containing species, a new probe -N3 was developed, in which para-azidobenzenyl ketone was covalently incorporated to a coumarin fluorophore linked by a C=C double bond. Sulfite and sulfide can respectively react with the C=C double bond and the azido group to give different products, consequently, they can be differentially identified by UV-vis and fluorescence spectroscopy as well as by the naked eye. Selectivity and competition results reveal that -N3 is a good candidate for the detection of sulfide and sulfite. The bioimaging experiment demonstrates the potential of the -N3 probe for the differential imaging of sulfide and sulfite in living cells.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China. junhongqian@ecust.edu.cn weibingzhang@ecust.edu.cn.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Tian
authors.forename: Junhong
authors.initials: J
authors.lastname: Qian
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Chenjia
authors.initials: C
authors.lastname: Jiang
authors.forename: Runsheng
authors.initials: R
authors.lastname: Zhang
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008823:Micelles; D061848:Optical Imaging; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000032:analysis*; D013447:Sulfites / Q000032:analysis*
chemical_list: D002593:Cetrimonium Compounds; D003374:Coumarins; D005456:Fluorescent Dyes; D008823:Micelles; D013440:Sulfides; D013447:Sulfites; C030123:coumarin; D000077286:Cetrimonium