title: Forensic informativity of ~3000 bp of coding sequence of domestic dog mtDNA.
abstract: The discriminatory power of the noncoding control region (CR) of domestic dog mitochondrial DNA alone is relatively low. The extent to which the discriminatory power could be increased by analyzing additional highly variable coding regions of the mitochondrial genome (mtGenome) was therefore investigated. Genetic variability across the mtGenome was evaluated by phylogenetic analysis, and the three most variable ~1 kb coding regions identified. We then sampled 100 Swedish dogs to represent breeds in accordance with their frequency in the Swedish population. A previously published dataset of 59 dog mtGenomes collected in the United States was also analyzed. Inclusion of the three coding regions increased the exclusion capacity considerably for the Swedish sample, from 0.920 for the CR alone to 0.964 for all four regions. The number of mtDNA types among all 159 dogs increased from 41 to 72, the four most frequent CR haplotypes being resolved into 22 different haplotypes.
authors.affiliation: Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, School of Biotechnology, SE-171 65, Solna, Sweden.
authors.forename: Helen
authors.initials: H
authors.lastname: Angleby
authors.forename: Mattias
authors.initials: M
authors.lastname: Oskarsson
authors.forename: Junfeng
authors.initials: J
authors.lastname: Pang
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Thomas
authors.initials: T
authors.lastname: Leitner
authors.forename: Caitlyn
authors.initials: C
authors.lastname: Braham
authors.forename: Lars
authors.initials: L
authors.lastname: Arvestad
authors.forename: Joakim
authors.initials: J
authors.lastname: Lundeberg
authors.forename: Kristen M
authors.initials: KM
authors.lastname: Webb
authors.forename: Peter
authors.initials: P
authors.lastname: Savolainen
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D030561:Databases, Nucleic Acid; D004285:Dogs / Q000235:genetics*; D016678:Genome; D006239:Haplotypes; D019901:Locus Control Region; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial
keywords: coding region; control region; domestic dog; exclusion capacity; forensic science; mitochondrial DNA