title: Absence of Association between CCR5 rs333 Polymorphism and Childhood Acute Lymphoblastic Leukemia.
abstract: Acute lymphoblastic leukemia (ALL) is a malignant disorder that originates from one single hematopoietic precursor committed to B- or T-cell lineage. Ordinarily, these cells express CCR5 chemokine receptor, which directs the immune response to a cellular pattern and is involved in cancer pathobiology. The genetic rs333 polymorphism of CCR5 (Δ32), results in a diminished receptor expression, thus leading to impaired cell trafficking. The objective of the present study was to investigate the effect of CCR5 chemokine receptor rs333 polymorphism in the pathogenesis of ALL. The genotype distribution was studied in 79 patients and compared with 80 control subjects, in a childhood population of Southern Brazil. Genotyping was performed using DNA samples amplified by polymerase chain reaction with sequence-specific primers (PCR-SSP). The homozygous (Δ32/Δ32) deletion was not observed in any subject involved in the study. Heterozygous genotype was not associated with ALL risk (OR 0.7%; 95% CI 0.21-2.32; P > 0.05), nor recurrence status of ALL (OR 0.86; 95% CI 0.13-5.48; P > 0.05). This work demonstrated, for the first time, no significant differences in the frequency of the CCR5/Δ32 genotype between ALL and control groups, indicating no effect of this genetic variant on the ALL susceptibility and recurrence risk.
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, (PR 445), Km 380, 86051-970 Londrina, PR, Brazil.
authors.forename: Carlos Eduardo Coral
authors.initials: CE
authors.lastname: de Oliveira
authors.affiliation: Laboratory of Hematology, Department of Pathology, Clinical and Toxicological Analysis, Health Sciences Center, State University of Londrina, Londrina, PR, Brazil.
authors.forename: Marla Karine
authors.identifier: 0000-0001-8218-8518
authors.initials: MK
authors.lastname: Amarante
authors.affiliation: Laboratory of Hematology, Department of Pathology, Clinical and Toxicological Analysis, Health Sciences Center, State University of Londrina, Londrina, PR, Brazil.
authors.forename: Aparecida de Lourdes
authors.initials: Ade L
authors.lastname: Perim
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, (PR 445), Km 380, 86051-970 Londrina, PR, Brazil.
authors.forename: Patricia Midori Murobushi
authors.initials: PM
authors.lastname: Ozawa
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, (PR 445), Km 380, 86051-970 Londrina, PR, Brazil.
authors.forename: Carlos
authors.identifier: 0000-0002-1343-5957
authors.initials: C
authors.lastname: Hiroki
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, (PR 445), Km 380, 86051-970 Londrina, PR, Brazil.
authors.forename: Glauco Akelinghton
authors.initials: GA
authors.lastname: Freire Vitiello
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, (PR 445), Km 380, 86051-970 Londrina, PR, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Losi Guembarovski
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, (PR 445), Km 380, 86051-970 Londrina, PR, Brazil.
authors.forename: Maria Angelica Ehara
authors.initials: MA
authors.lastname: Watanabe