title: Recombinant nAG (a salamander-derived protein) decreases the formation of hypertrophic scarring in the rabbit ear model.
abstract: nAG (newt-Anterrior Gradient) protein is the key mediator of regrowth of amputated limbs in salamanders. In a previous work in our lab, a new nAG gene (suitable for humans) was designed and cloned. The cloned vector was transfected into primary human fibroblasts. The expression of nAG in human primary fibroblasts was found to suppress collagen expression. The current study shows that local injection of recombinant nAG reduces scar hypertrophy in the rabbit ear model. This is associated with lower scar elevation index (SEI), lower levels of collagen I & III, higher levels of MMP1, and a higher degree of scar maturation in experimental wounds compared to controls.
authors.affiliation: Department of Surgery, King Saud University, PO Box 18097, Riyadh 11415, Saudi Arabia ; College of Medicine Research Center, King Saud University, PO Box 18097, Riyadh 11415, Saudi Arabia.
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Al-Qattan
authors.affiliation: College of Medicine Research Center, King Saud University, PO Box 18097, Riyadh 11415, Saudi Arabia.
authors.forename: Mervat M
authors.initials: MM
authors.lastname: Abd-Al Wahed
authors.affiliation: Department of Surgery, King Saud University, PO Box 18097, Riyadh 11415, Saudi Arabia.
authors.forename: Khalid
authors.initials: K
authors.lastname: Hawary
authors.affiliation: Department of Pathology, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Alhumidi
authors.affiliation: College of Medicine Research Center, King Saud University, PO Box 18097, Riyadh 11415, Saudi Arabia.
authors.forename: Medhat K
authors.initials: MK
authors.lastname: Shier
mesh_terms: D029845:Amphibian Proteins / Q000008:administration & dosage* / Q000235:genetics; D000818:Animals; D001688:Biological Products / Q000008:administration & dosage*; D017439:Cicatrix, Hypertrophic / Q000188:drug therapy* / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D004427:Ear Diseases / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D015552:Injections, Intralesional; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage; D016896:Treatment Outcome; D014562:Caudata / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D029845:Amphibian Proteins; D001688:Biological Products; D011994:Recombinant Proteins; D003094:Collagen