title: [Cloning, expression and analysis of Der f Mag 29 allergen of Dermatophagoides farinae].
abstract: The full-length Mag 29 gene of Dermatophagoides farinae was amplified by RT-PCR with a pair of specific primers. The PCR product was cloned into pCold TF DNA vector. The constructed plasmid pCold TF-Mag 29 was transformed into E. coli BL21 and followed by expression of the protein induced by IPTG. The recombinant protein was analyzed by SDS-PAGE. The full-length Mag 29 gene was 429 bp. A specific band (Mr 63,000) were detected in the whole cells, the supernatant, and the precipitate. Bioinformatics analysis revealed that Mag 29 protein was composed with 142 amino acid residues with a calculated molecular weight of Mr 15,100, and its secondary structure was composed of alpha helix (55.63%), extended strand (3.52%), and random coil (40.85%). The Mag 29 allergen was a hydrophilic and cytoplasmic protein, and shared a high degree homology with the heat shock protein 70 family.
authors.forename: Jin-Xia
authors.initials: JX
authors.lastname: Sun
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Bian
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Yu-Bao
authors.initials: YB
authors.lastname: Cui
mesh_terms: D000485:Allergens / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D040001:Dermatophagoides farinae / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D010957:Plasmids; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D011994:Recombinant Proteins