title: Perchlorate reduction by hydrogen autotrophic bacteria in a bioelectrochemical reactor.
abstract: The autotrophic hydrogen reduction of perchlorate was investigated in batch modes in a proton-exchange membrane bioelectrochemical reactor. The phylogenic characterization of hydrogen-autotrophic perchlorate-reducing cultures in the cathode cell mainly included the genera Aureibacter tunicatorum, Fulvivirga kasyanovii, Thermotalea metallivorans (T), bacterium WHC2-6, and Thauera sp. Q20-C. The suitable H2SO4 supplement concentration was identified to be between 0.04 and 0.05 mol L(-1) in the anode cell. The perchlorate reduction was affected by the sludge concentration in the cathode cell, current intensity, and initial perchlorate concentration. The removal efficiency of perchlorate positively correlated with the current intensity, and the current intensity of 20, 40, 50, and 60 mA corresponded to removal efficiencies of 95.03, 96.29, 97.56 and 98.99%, respectively. When the current intensity was sufficient for hydrogen production, the kinetics of the perchlorate reduction conformed to a zero-order kinetics model, and the maximum specific substrate utilization rates for perchlorate (Vmax) ranged from 5.52 to 14.34 mg ClO4(-) g(-1) volatile suspended solid (VSS) h(-1).
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Zichao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. Electronic address: mengchungao@hotmail.com.
authors.forename: Mengchun
authors.initials: M
authors.lastname: Gao
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: You
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Zonglian
authors.initials: Z
authors.lastname: She
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Zhao
mesh_terms: D052818:Autotrophic Processes; D001419:Bacteria / Q000378:metabolism*; D019149:Bioreactors; D004563:Electrochemistry; D004566:Electrodes; D006859:Hydrogen / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D010084:Oxidation-Reduction; D010472:Perchlorates / Q000378:metabolism*; D012722:Sewage; D013464:Sulfuric Acids / Q000737:chemistry
chemical_list: D010472:Perchlorates; D012722:Sewage; D013464:Sulfuric Acids; D006859:Hydrogen; C033158:sulfuric acid; C494474:perchlorate
keywords: Bioelectrochemical reactor; Current intensity; H(2)SO(4) supplement; Perchlorate