title: Fluorescent amplified fragment length polymorphism (fAFLP) analysis of Listeria monocytogenes.
abstract: Fluorescent amplified fragment length polymorphism (fAFLP) is based on the selective PCR amplification of restriction fragments from a digest of total genomic DNA. Genomic DNA extracted from a purified bacterial isolate is completely digested with two endonucleases generating fragments which are ligated to specific double-stranded adaptors. The ligated fragments are then amplified by PCR using fluorescently labelled primers. Fluorescent amplified fragments are separated by size on an automated sequencer with a size standard. fAFLP is a rapid, highly reproducible technique which can be used to discriminate and subtype Listeria monocytogenes strains.
authors.affiliation: Gastrointestinal Bacteria Reference Unit, Public Health England, 61 Colindale Avenue, London, NW9 5EQ, UK, corinne.Amar@phe.gov.uk.
authors.forename: Corinne
authors.initials: C
authors.lastname: Amar
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000191:economics / Q000379:methods*; D015373:Bacterial Typing Techniques / Q000191:economics / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D005453:Fluorescence; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics*; D008088:Listeriosis / Q000382:microbiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial