title: Rapid separation and identification of   Strychnos alkaloids metabolites in rats by   ultra high performance liquid   chromatography with linear ion trap Orbitrap   mass spectrometry.
abstract: An in vivo study of Strychnos alkaloids metabolites in rats by ultra high performance liquid chromatography with linear ion trap Orbitrap MS is reported for the first time. Two major Strychnos alkaloids compounds including strychnine and brucine were investigated. To obtain optimal extraction efficiency, samples were pretreated by using an SPE plate. The structures of metabolites and their fragment ions were characterized based on the accurate mass and MSn data. Forty-seven metabolites were identified in rat urine, of which 25 were reported for the first time. Four new metabolism pathways were proposed on the basis of the identified metabolites. This study provides a practical approach for rapidly identifying complicated metabolites, a methodology that could be widely applied not only in forensic and clinically toxicological relevant cases, but also for the structural characterization of metabolites of other compounds.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Liu
authors.forename: Shuiqing
authors.initials: S
authors.lastname: Zheng
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
authors.forename: Chen
authors.initials: C
authors.lastname: Liang
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhou
authors.forename: Yurong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yunqiu
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D017207:Rats, Sprague-Dawley; D031451:Strychnos / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000470:Alkaloids