title: Membrane proteomics of Pseudomonas aeruginosa.
abstract: In recent years gel-free proteomics approaches have been increasingly used for global quantitative proteome analyses of multiple prokaryotic organisms, including Pseudomonas aeruginosa. A major advantage of this method is its suitability for the investigation of membrane proteomes. In this chapter, we present a protocol for preparation of proteins from the inner and outer membrane of P. aeruginosa PAO1 grown as a biofilm culture. Parameters for quantitative protein measurements by 2D-LC-MS/MS are described.
authors.affiliation: Microbial Proteomics, Helmholtz Centre for Infection Research (HZI), Inhoffenstr, Braunschweig, Germany.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Magnowska
authors.forename: Isabel
authors.initials: I
authors.lastname: Hartmann
authors.forename: Lothar
authors.initials: L
authors.lastname: Jänsch
authors.forename: Dieter
authors.initials: D
authors.lastname: Jahn
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002254:Carbonates; D002412:Cations; D002500:Centrifugation, Isopycnic; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007553:Isotope Labeling; D008565:Membrane Proteins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002254:Carbonates; D002412:Cations; D008565:Membrane Proteins; D010455:Peptides