title: Development and characterization of liposomal doxorubicin hydrochloride with palm oil.
abstract: The usage of natural products in pharmaceuticals has steadily seen improvements over the last decade, and this study focuses on the utilization of palm oil in formulating liposomal doxorubicin (Dox). The liposomal form of Dox generally minimizes toxicity and enhances target delivery actions. Taking into account the antiproliferative and antioxidant properties of palm oil, the aim of this study is to design and characterize a new liposomal Dox by replacing phosphatidylcholine with 5% and 10% palm oil content. Liposomes were formed using the freeze_thaw method, and Dox was loaded through pH gradient technique and characterized through in vitro and ex vivo terms. Based on TEM images, large lamellar vesicles (LUV) were formed, with sizes of 438 and 453 nm, having polydispersity index of 0.21 ± 0.8 and 0.22 ± 1.3 and zeta potentials of about -31 and -32 mV, respectively. In both formulations, the entrapment efficiency was about 99%, and whole Dox was released through 96 hours in PBS (pH = 7.4) at 37°C. Comparing cytotoxicity and cellular uptake of LUV with Caelyx(R) on MCF7 and MDA-MBA 231 breast cancer cell lines indicated suitable uptake and lower IC50 of the prepared liposomes.
authors.affiliation: Department of Pharmacy, Faculty of Medicine, University of Malaya, 51200 Kuala Lumpur, Malaysia.
authors.forename: Bahareh
authors.initials: B
authors.lastname: Sabeti
authors.affiliation: Department of Pharmacy, Faculty of Medicine, University of Malaya, 51200 Kuala Lumpur, Malaysia.
authors.forename: Mohamed Ibrahim
authors.initials: MI
authors.lastname: Noordin
authors.affiliation: Faculty of Pharmacy, Cyberjaya University College of Medical Sciences, 63000 Cyberjaya, Selangor, Malaysia.
authors.forename: Shaharuddin
authors.initials: S
authors.lastname: Mohd
authors.affiliation: Faculty of Pharmacy, Cyberjaya University College of Medical Sciences, 63000 Cyberjaya, Selangor, Malaysia.
authors.forename: Rosnani
authors.initials: R
authors.lastname: Hashim
authors.affiliation: Faculty of Pharmacy, Cyberjaya University College of Medical Sciences, 63000 Cyberjaya, Selangor, Malaysia.
authors.forename: Afendi
authors.identifier: 0000-0001-7491-2457
authors.initials: A
authors.lastname: Dahlan
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 141176, Iran.
authors.forename: Hamid Akbari
authors.initials: HA
authors.lastname: Javar
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D000073878:Palm Oil; D010316:Particle Size; D010938:Plant Oils / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D000903:Antibiotics, Antineoplastic; D008081:Liposomes; D010938:Plant Oils; C506643:liposomal doxorubicin; D011092:Polyethylene Glycols; D000073878:Palm Oil; D004317:Doxorubicin