title: Isolation of 12 microsatellite markers following a pyrosequencing procedure and cross-priming in two invasive cryptic species, Alexandrium catenella (group IV) and A. tamarense (group III) (Dinophyceae).
abstract: Alexandrium catenella (group IV) and Alexandrium tamarense (group III) (Dinophyceae) are two cryptic invasive phytoplankton species belonging to the A. tamarense species complex. Their worldwide spread is favored by the human activities, transportation and climate change. In order to describe their diversity in the Mediterranean Sea and understand their settlements and maintenances in this area, new microsatellite markers were developed based on Thau lagoon (France) samples of A. catenella and A. tamarense strains. In this study twelve new microsatellite markers are proposed. Five of these microsatellite markers show amplifications on A. tamarense and ten on A. catenella. Three of these 12 microsatellite markers allowed amplifications on both cryptic species. Finally, the haplotypic diversity ranged from 0.000 to 0.791 and 0.000 to 0.942 for A. catenella and A. tamarense respectively.
authors.affiliation: Institut des Sciences de l'Evolution de Montpellier, UMR 5554 UM2-CNRS-IRD, Université Montpellier II, CC065, Place E. Bataillon, 34095 Montpellier, Cedex 5, France; Département de Biologie, IBIS (Institut de Biologie Intégrative et des Systèmes), Université Laval, Québec G1V 0A6, Canada.
authors.forename: Martin
authors.initials: M
authors.lastname: Laporte
authors.affiliation: Institut des Sciences de l'Evolution de Montpellier, UMR 5554 UM2-CNRS-IRD, Université Montpellier II, CC065, Place E. Bataillon, 34095 Montpellier, Cedex 5, France.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Shao
authors.affiliation: Institut des Sciences de l'Evolution de Montpellier, UMR 5554 UM2-CNRS-IRD, Université Montpellier II, CC065, Place E. Bataillon, 34095 Montpellier, Cedex 5, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Berrebi
authors.affiliation: Ecologie des Systèmes Marins Côtiers, UMR 5119 UM2-CNRS-IRD-Ifremer-UM1, Université Montpellier II, CC 093, 34095 Montpellier Cedex 5, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Laabir
authors.affiliation: Laboratoire Environnement Ressources Languedoc-Roussillon Ifremer, B.P. 171, 34203 Sète, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Abadie
authors.affiliation: Ecologie des Systèmes Marins Côtiers, UMR 5119 UM2-CNRS-IRD-Ifremer-UM1, Université Montpellier II, CC 093, 34095 Montpellier Cedex 5, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Faivre
authors.affiliation: Ecologie des Systèmes Marins Côtiers, UMR 5119 UM2-CNRS-IRD-Ifremer-UM1, Université Montpellier II, CC 093, 34095 Montpellier Cedex 5, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Rieuvilleneuve
authors.affiliation: Ecologie des Systèmes Marins Côtiers, UMR 5119 UM2-CNRS-IRD-Ifremer-UM1, Université Montpellier II, CC 093, 34095 Montpellier Cedex 5, France. Electronic address: estelle.masseret@univ-montp2.fr.
authors.forename: Estelle
authors.initials: E
authors.lastname: Masseret
mesh_terms: D004141:Dinoflagellida / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D058865:Introduced Species; D008522:Mediterranean Sea; D018895:Microsatellite Repeats; D010839:Phytoplankton; D017422:Sequence Analysis, DNA
keywords: Alexandrium; Biogeography; Dinophyceae; Invasive species; Microsatellite markers; Phytoplankton