title: ESR1 and PGR gene promoter methylation and correlations with estrogen and progesterone receptors in ductal and lobular breast cancer.
abstract: The aim of this work was to analyze methylation of the promoter sites of the ESR1 and PGR genes and to determine correlations with immunohistochemical expression of estrogen and progesterone receptors in ductal and lobular breast cancers. An observational, descriptive, molecular study was conducted on 20 ductal and 20 lobular breast cancer samples with immunohistochemical determination of estrogen and progesterone receptor expression. The methylation analysis of ESR1 and PGR promoter sites was carried-out by methylation-specific PCR. For correlation analysis, Kendall's tau coefficient was determined. Positive correlations were found between estrogen and progesterone receptors, estrogen receptor and unmethylated progesterone receptor, progesterone receptor, and unmethylated progesterone receptor. Negative correlations were found between estrogen receptor and methylated progesterone receptor, progesterone receptor and methylated progesterone receptor, methylated and unmethylated estrogen receptor, and methylated and unmethylated progesterone receptor. The results suggest that methylation of promoter sites of ESR1 and PGR is a relatively uncommon event in ductal and lobular breast cancer, and also suggest that the determination of epigenetic states of ESR1 and PGR could represent an alternative or complement to the histopathological expression analysis.
authors.affiliation: Health Services of Durango SSD, Durango, Mexico E-mail : sifual55@hotmail.com.
authors.forename: Alma Delia
authors.initials: AD
authors.lastname: Medina-Jaime
authors.forename: Francianella
authors.initials: F
authors.lastname: Reyes-Vargas
authors.forename: Victoria
authors.initials: V
authors.lastname: Martinez-Gaytan
authors.forename: Graciela
authors.initials: G
authors.lastname: Zambrano-Galvan
authors.forename: Eduardo
authors.initials: E
authors.lastname: Portillo-Delcampo
authors.forename: Jorge Alberto
authors.initials: JA
authors.lastname: Burciaga-Nava
authors.forename: Miguel
authors.initials: M
authors.lastname: Reyes-Romero
authors.forename: Antonio
authors.initials: A
authors.lastname: Sifuentes-Alvarez
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D018275:Carcinoma, Lobular / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics*; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011960:Receptors, Estrogen / Q000235:genetics; D011980:Receptors, Progesterone / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D047908:Intracellular Signaling Peptides and Proteins; C439067:MRFAP1 protein, human; D009687:Nuclear Proteins; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone