title: Analysis of methylated patterns and quality-related genes in tobacco (Nicotiana tabacum) cultivars.
abstract: Methylation-sensitive amplified polymorphism was used in this study to investigate epigenetic information of four tobacco cultivars: Yunyan 85, NC89, K326, and Yunyan 87. The DNA fragments with methylated information were cloned by reamplified PCR and sequenced. The results of Blast alignments showed that the genes with methylation information included chitinase, nitrate reductase, chloroplast DNA, mitochondrial DNA, ornithine decarboxylase, ribulose carboxylase, and promoter sequences. Homologous comparison in three cloned gene sequences (nitrate reductase, ornithine decarboxylase, and ribulose decarboxylase) indicated that geographic factors had significant influence on the whole genome methylation. Introns also contained different information in different tobacco cultivars. These findings suggest that synthetic mechanisms for tobacco aromatic components could be affected by different environmental factors leading to variation of noncoding regions in the genome, which finally results in different fragrance and taste in different tobacco cultivars.
authors.affiliation: School of Basic Medicine, Xinxiang Medical University, Xinxiang, 453003, Henan, China, jiaojn@yeah.net.
authors.forename: Junna
authors.initials: J
authors.lastname: Jiao
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Jia
authors.forename: Zhuangwei
authors.initials: Z
authors.lastname: Lv
authors.forename: Chuanfei
authors.initials: C
authors.lastname: Sun
authors.forename: Lijie
authors.initials: L
authors.lastname: Gao
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Yan
authors.forename: Liusu
authors.initials: L
authors.lastname: Cui
authors.forename: Zongxiang
authors.initials: Z
authors.lastname: Tang
authors.forename: Benju
authors.initials: B
authors.lastname: Yan
mesh_terms: D019175:DNA Methylation; D017343:Genes, Plant; D005819:Genetic Markers; D007438:Introns; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000235:genetics*
chemical_list: D005819:Genetic Markers