title: Rapid and parallel quantification of small and large RNA species.
abstract: Quantitative real-time PCR (qRT-PCR) is a common technique for mRNA quantification. Several methods have been developed in the past few years in order to adapt qRT-PCR also for small non-coding RNAs (sRNA). We here provide a simple and sensitive protocol that allows quantification of mRNAs, selected sRNAs, and long non-coding RNAs (lncRNA) in one cDNA sample by qRT-PCR.
authors.affiliation: Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany.
authors.forename: Corinna
authors.initials: C
authors.lastname: Speth
authors.forename: Sascha
authors.initials: S
authors.lastname: Laubinger
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D012313:RNA / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D018749:RNA, Plant; D012313:RNA