title: Multiple sclerosis: the elevated antibody response to Epstein-Barr virus primarily targets, but is not confined to, the glycine-alanine repeat of Epstein-Barr nuclear antigen-1.
abstract: Patients with multiple sclerosis (MS) have elevated antibodies against Epstein-Barr virus (EBV), but data on the epitope-resolved specificity of these antibodies are scarce. Using a peptide microarray containing 1465 peptides representing 8 full-length EBV proteins, we identified higher (p<0.001) antibody reactivities to 39 EBV-peptides in MS patients (n=29) compared to healthy controls (n=22). Seventeen of the 39 peptides were from EBNA-1 and 13 located within the glycine-alanine repeat of EBNA-1. Further reactivities were directed against EBNA-3, EBNA-4, EBNA-6, VP26, and LMP1. Thus, antibodies against EBV in MS patients primarily target, but are not confined to, the glycine-alanine repeat of EBNA-1.
authors.affiliation: Department of Neurology, Charité - Universitätsmedizin Berlin, Germany; Clinical and Experimental Multiple Sclerosis Research Center, Charité - Universitätsmedizin Berlin, Germany. Electronic address: klemens.ruprecht@charite.de.
authors.forename: Klemens
authors.initials: K
authors.lastname: Ruprecht
authors.affiliation: Department of Neurology, Charité - Universitätsmedizin Berlin, Germany; Clinical and Experimental Multiple Sclerosis Research Center, Charité - Universitätsmedizin Berlin, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Wunderlich
authors.affiliation: Department of Neurology, Charité - Universitätsmedizin Berlin, Germany; Clinical and Experimental Multiple Sclerosis Research Center, Charité - Universitätsmedizin Berlin, Germany.
authors.forename: René
authors.initials: R
authors.lastname: Gieß
authors.affiliation: JPT Peptide Technologies GmbH, Berlin, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Meyer
authors.affiliation: Institute for Medical Immunology, Charité - Universitätsmedizin Berlin, Germany.
authors.forename: Madlen
authors.initials: M
authors.lastname: Loebel
authors.affiliation: Institute of Medical Biometrics and Clinical Epidemiology, Charité - Universitätsmedizin Berlin, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Lenz
authors.affiliation: Labor Berlin Charité-Vivantes GmbH, Berlin, Germany; Institute of Medical Virology, Charité - Universitätsmedizin Berlin, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Hofmann
authors.affiliation: Department of Neurology, Charité - Universitätsmedizin Berlin, Germany; Clinical and Experimental Multiple Sclerosis Research Center, Charité - Universitätsmedizin Berlin, Germany.
authors.forename: Berit
authors.initials: B
authors.lastname: Rosche
authors.affiliation: Department of Neurology, Charité - Universitätsmedizin Berlin, Germany; Clinical and Experimental Multiple Sclerosis Research Center, Charité - Universitätsmedizin Berlin, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Wengert
authors.affiliation: Department of Neurology, Charité - Universitätsmedizin Berlin, Germany; Clinical and Experimental Multiple Sclerosis Research Center, Charité - Universitätsmedizin Berlin, Germany; NeuroCure Clinical Research Center, Charité - Universitätsmedizin Berlin, Germany.
authors.forename: Friedemann
authors.initials: F
authors.lastname: Paul
authors.affiliation: JPT Peptide Technologies GmbH, Berlin, Germany.
authors.forename: Ulf
authors.initials: U
authors.lastname: Reimer
authors.affiliation: Institute for Medical Immunology, Charité - Universitätsmedizin Berlin, Germany.
authors.forename: Carmen
authors.initials: C
authors.lastname: Scheibenbogen
mesh_terms: D000328:Adult; D000409:Alanine / Q000276:immunology; D000917:Antibody Formation; D000956:Antigens, Viral / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D020031:Epstein-Barr Virus Infections / Q000097:blood / Q000276:immunology*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000276:immunology*; D005260:Female; D005998:Glycine / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology*; D012372:ROC Curve; D055815:Young Adult
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D019309:Epstein-Barr Virus Nuclear Antigens; D000409:Alanine; D005998:Glycine
keywords: Antibodies; Epstein–Barr nuclear antigen-1; Epstein–Barr virus; Glycine–alanine repeat; Multiple sclerosis; Peptide array