title: An evidence in vitro for the influence of bisphenol A on uterine leiomyoma.
abstract: OBJECTIVE
  To study the effect and mechanism of environmental estrogens bisphenol A (BPA) on uterine leiomyoma (UL) cells.
  
  
  METHODS
  Primary cultures and subcultures of human UL cells, which were identified by immunocytochemical staining with a monoclonal anti-α-smooth muscle actin antibody, were performed. The viability of cells in different concentration of bisphenol A of 24h, 48h and 72h were analyzed by CCK-8. The expressions of mRNA of ERα, IGF-1 and VEGF in all groups were detected by real-time quantitative PCR assay, and then the expressions of proteins detected by Western blot assay.
  
  
  RESULTS
  BPA promoted the growth of UL cells and the expressions of ERα, IGF-1 and VEGF, which had positive correlation with the concentration and action time of BPA treatment.
  
  
  CONCLUSIONS
  BPA promotes the growth of leiomyoma cells. The expressions of IGF-1, VEGF can be up-regulated by ERα, which may be possible mechanism of BPA promote the growth of leiomyoma cells.
authors.affiliation: Department of Obstetrics and Gynecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China. Electronic address: shenyang0924@sina.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Obstetrics and Gynecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Mu-Lan
authors.initials: ML
authors.lastname: Ren
authors.affiliation: Department of Obstetrics and Gynecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Obstetrics and Gynecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Yun-Lang
authors.initials: YL
authors.lastname: Cai
authors.affiliation: Department of Obstetrics and Gynecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Yong-Xing
authors.initials: YX
authors.lastname: Gao
authors.affiliation: School of Public Health, Southeast University, Nanjing 210009, China; Ministry of Education Key Laboratory of Environmental Medicine Engineering, Southeast University, Nanjing 210009, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
mesh_terms: D001559:Benzhydryl Compounds / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D047628:Estrogen Receptor alpha / Q000096:biosynthesis; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis; D007889:Leiomyoma / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D047628:Estrogen Receptor alpha; D004967:Estrogens; D007334:Insulin-Like Growth Factor I; D010636:Phenols; D042461:Vascular Endothelial Growth Factor A; C506487:ESR1 protein, human; C467484:VEGFA protein, human; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; ERα; Environmental estrogen; IGF-1; Uterine leiomyoma; VEGF