title: Effects of subchronic exposure to N,N-diethyl-m-toluamide on selected biomarkers in common carp (Cyprinus carpio L.).
abstract: DEET (N,N-diethyl-m-toluamide) is the most common active ingredient in the insect repellents commonly detected in European groundwater. The aim of this study was to investigate the effect of subchronic DEET exposure on biochemical and haematological parameters, antioxidant enzymes, including catalase, glutathione peroxidase, glutathione reductase, and glutathione S-transferase, and the amount of thiobarbituric acid reactive substances (TBARS) in common carp (Cyprinus carpio L.). Two specific proinflammatory and anti-inflammatory cytokine genes were selected to assess an immunological status of the fish. Fish were exposed for 28 days to three concentrations of DEET (1.0 µg/L, 0.1 mg/L, and 1.0 mg/L) where 1 µg/L is corresponding to the concentration found in the environment. DEET had a significant (P < 0.05) effect on increased RBC, decreased mean corpuscular volume (MCV), and mean corpuscular haemoglobin value (MCH) compared to control groups in the concentration of 1 mg/L. A significant decline (P < 0.05) in triacylglycerols (TAG) in plasma was found in the concentration of 1 mg/L compared to the control groups. The parameters of oxidative stress in tissues of common carp were weekly affected and immunological parameters were not affected.
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Slaninova
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Modra
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Hostovsky
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
authors.forename: Eliska
authors.initials: E
authors.lastname: Sisperova
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Blahova
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
authors.forename: Iveta
authors.initials: I
authors.lastname: Matejova
authors.affiliation: Department of Immunology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Vicenova
authors.affiliation: Department of Immunology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Faldyna
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Zelnickova
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Tichy
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Svobodova
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D015415:Biomarkers / Q000097:blood*; D002347:Carps / Q000378:metabolism*; D016207:Cytokines / Q000097:blood; D003671:DEET / Q000008:administration & dosage / Q000633:toxicity*; D009928:Organ Specificity; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000097:blood; D059550:Toxicity Tests, Subchronic
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016207:Cytokines; D003671:DEET; D010088:Oxidoreductases