title: [Cloning and prokaryotic expression of malate dehydrogenase gene of Taenia saginata asiatica and immunogenicity analysis of the recombinant protein].
abstract: OBJECTIVE
  To clone and express the lactate dehydrogenase (LDH) gene of Taenia saginata asiatica and analyze the immunogenicity of the recombinant protein.
  
  
  METHODS
  By screening the full length cDNA plasmid library, the coding region of LDH was amplified with PCR, and cloned into the prokaryotic expression vector pET-30a (+), then expressed in E. coli BL21 with IPTG induction. The recombinant protein was detected by SDS-PAGE and purified by Ni-IDA affinity chromatography, and its immunogenicity was analyzed by Western blotting.
  
  
  RESULTS
  PCR, double enzyme digestion and DNA sequencing confirmed that the recombinant expression plasmid was constructed. The expression products were obtained and purified by Ni-IDA affinity chromatography. Western blotting analysis of LDH recombinant protein testified that the recombinant protein could be recognized by sera of the Taenia saginata asiatica infected swine and the patient.
  
  
  CONCLUSIONS
  The LDH gene of Taenia saginata asiatica has been cloned and expressed, and the purified protein has been confirmed with immunogenicity.
authors.forename: Jiang
authors.initials: J
authors.lastname: Huang
authors.forename: Xu-Chu
authors.initials: XC
authors.lastname: Hu
authors.forename: Xuan
authors.initials: X
authors.lastname: Wu
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Xin-Bing
authors.initials: XB
authors.lastname: Yu
authors.forename: Huai-En
authors.initials: HE
authors.lastname: Bao
authors.forename: Shu-Yuan
authors.initials: SY
authors.lastname: Lang
authors.forename: Xing-Jiang
authors.initials: XJ
authors.lastname: Liao
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008291:Malate Dehydrogenase / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013552:Swine / Q000469:parasitology*; D041202:Taenia saginata / Q000201:enzymology*
chemical_list: D011994:Recombinant Proteins; D008291:Malate Dehydrogenase