title: Hypothalamic protein profiles associated with inhibited feed intake of ducks fed with insufficient dietary arginine.
abstract: An experiment was conducted to investigate the effect of arginine on feed intake regulation. One hundred and twenty six 1-day-old male White Pekin ducks (Anas platyrhynchos domestica) were randomly were allotted to one of two dietary treatments. The birds were fed diets containing 0.71% (deficient) or 1.27% (sufficient) arginine for 3 weeks. At 21 days of age, feed intake was determined and hypothalamic protein profiles were analyzed using isobaric tags for relative and absolute quantification technique. The birds fed with arginine-deficient diet had a lower final live BW and cumulative feed intake (P1.5-fold expressional changes between arginine-deficient and -sufficient dietary treatments. Nine of these proteins were upregulated and seven of them were downregulated. The identified proteins could be regrouped into six categories: protein processing, carbohydrate metabolism and energy production, transporter, cytoskeleton, immunity and neuronal development. Dietary arginine deficiency decreased expression of proteins involved in energy production (glycine amidinotransferase, aldolase B fructose-bisphosphate, aconitase, transaldolase, 6-phosphofructokinase type C-like) and oxygen transportation (haemoglobin subunit α expression). The proteomic alterations described here provides valuable insights into the interactions of arginine with appetite.
authors.affiliation: 1Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China.
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.affiliation: 3Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China.
authors.forename: A J
authors.initials: AJ
authors.lastname: Zheng
authors.affiliation: 1Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China.
authors.forename: M
authors.initials: M
authors.lastname: Xie
authors.affiliation: 1Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China.
authors.forename: W
authors.initials: W
authors.lastname: Huang
authors.affiliation: 1Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Xie
authors.affiliation: 1Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China.
authors.forename: S S
authors.initials: SS
authors.lastname: Hou
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena / Q000187:drug effects; D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000172:deficiency*; D004032:Diet / Q000662:veterinary*; D004305:Dose-Response Relationship, Drug; D004372:Ducks / Q000254:growth & development / Q000502:physiology*; D008297:Male; D040901:Proteomics
chemical_list: D001120:Arginine