title: Mycobacterium hippocampi sp. nov., a rapidly growing scotochromogenic species isolated from a seahorse with tail rot.
abstract: A Gram-positive, aerobic, non-motile, non-sporulating, acid-fast, and rod-shaped bacterium (BFLP-6(T)), previously isolated from a seahorse (Hippocampus guttulatus) with tail rot, was studied using a polyphasic taxonomic approach. Growth occurred at 15-35 °C (optimum 25 °C), at pH 5.0-10.0 (optimum pH 7.0) and at NaCl concentrations between 0 and 6 % (w/v). The G+C content of DNA was 66.7 mol%. The predominant fatty acids were C(18:1) ω9c, C(16:0) and C(16:1) ω6c. A mycolic acid pattern of alpha-mycolates and keto-mycolates was detected. Analysis of concatenated sequences (16S rRNA, rpoB, ssrA and tuf genes), and chemotaxonomic and phenotypic features indicated that strain BFLP-6(T) represents a novel species within the genus Mycobacterium, for which the name Mycobacterium hippocampi sp. nov. is proposed. The type strain is BFLP-6(T) (=DSM 45391(T) =LMG 25372(T)).
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC), Eduardo Cabello 6, 36208, Vigo, Spain, jlbalcazar@icra.cat.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Balcázar
authors.forename: Miquel
authors.initials: M
authors.lastname: Planas
authors.forename: José
authors.initials: J
authors.lastname: Pintado
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009164:Mycobacterium Infections / Q000382:microbiology; D009171:Mycolic Acids / Q000032:analysis; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D023701:Smegmamorpha / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism; D013623:Tail / Q000382:microbiology; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit