title: Methods for studying biofilm dispersal in Pseudomonas aeruginosa.
abstract: Biofilm dispersal is the last and least understood stage of the biofilm life cycle. Several recent studies have characterized dispersal events in response to various cues and signals. Here we describe a range of methods useful for the investigation of dispersal in the biofilm model organism and opportunistic pathogen Pseudomonas aeruginosa.
authors.affiliation: Centre for Marine Bio-Innovation and School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW, Australia, n.barraud@unsw.edu.au.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Barraud
authors.forename: Joana A
authors.initials: JA
authors.lastname: Moscoso
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Ghigo
authors.forename: Alain
authors.initials: A
authors.lastname: Filloux
mesh_terms: D061249:Batch Cell Culture Techniques; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology; D015169:Colony Count, Microbial; D005840:Gentian Violet; D008828:Microbiological Techniques / Q000379:methods*; D009569:Nitric Oxide / Q000172:deficiency; D010100:Oxygen / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology*; D013194:Staining and Labeling
chemical_list: D009569:Nitric Oxide; D005840:Gentian Violet; D010100:Oxygen