title: Chromatin hydrodynamics.
abstract: Following recent observations of large scale correlated motion of chromatin inside the nuclei of live differentiated cells, we present a hydrodynamic theory-the two-fluid model-in which the content of a nucleus is described as a chromatin solution with the nucleoplasm playing the role of the solvent and the chromatin fiber that of a solute. This system is subject to both passive thermal fluctuations and active scalar and vector events that are associated with free energy consumption, such as ATP hydrolysis. Scalar events drive the longitudinal viscoelastic modes (where the chromatin fiber moves relative to the solvent) while vector events generate the transverse modes (where the chromatin fiber moves together with the solvent). Using linear response methods, we derive explicit expressions for the response functions that connect the chromatin density and velocity correlation functions to the corresponding correlation functions of the active sources and the complex viscoelastic moduli of the chromatin solution. We then derive general expressions for the flow spectral density of the chromatin velocity field. We use the theory to analyze experimental results recently obtained by one of the present authors and her co-workers. We find that the time dependence of the experimental data for both native and ATP-depleted chromatin can be well-fitted using a simple model-the Maxwell fluid-for the complex modulus, although there is some discrepancy in terms of the wavevector dependence. Thermal fluctuations of ATP-depleted cells are predominantly longitudinal. ATP-active cells exhibit intense transverse long wavelength velocity fluctuations driven by force dipoles. Fluctuations with wavenumbers larger than a few inverse microns are dominated by concentration fluctuations with the same spectrum as thermal fluctuations but with increased intensity.
authors.affiliation: Department of Physics and Astronomy, Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California. Electronic address: bruinsma@physics.ucla.edu.
authors.forename: Robijn
authors.initials: R
authors.lastname: Bruinsma
authors.affiliation: Department of Physics and Center for Soft Matter Research, New York University, New York, New York.
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Grosberg
authors.affiliation: Department of Physics, and Institute for Nanotechnology and Advanced Materials, Bar Ilan University, Ramat Gan, Israel.
authors.forename: Yitzhak
authors.initials: Y
authors.lastname: Rabin
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, Massachusetts; School of Engineering and Applied Sciences, Department of Physics, Harvard University, Cambridge, Massachusetts.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Zidovska
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002453:Cell Cycle; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D057446:Hydrodynamics; D008954:Models, Biological; D015398:Signal Transduction; D013816:Thermodynamics
chemical_list: D002843:Chromatin; D000255:Adenosine Triphosphate