title: MicroRNA profiling of activated and tolerogenic human dendritic cells.
abstract: Dendritic cells (DCs) belong to the immune system and are particularly studied for their potential to direct either an activated or tolerogenic immune response. The roles of microRNAs (miRNAs) in posttranscriptional gene expression regulation are being increasingly investigated. This study's aim is to evaluate the miRNAs' expression changes in prepared human immature (iDCs), activated (aDCs), and tolerogenic dendritic cells (tDCs). The dendritic cells were prepared using GM-CSF and IL-4 (iDC) and subsequently maturated by adding LPS and IFN-γ (aDC) or IL-10 and TGF-β (tDC). Surface markers, cytokine profiles, and miRNA profiles were evaluated in iDC, tDC, and aDC at 6 h and 24 h of maturation. We identified 4 miRNAs (miR-7, miR-9, miR-155 and miR-182), which were consistently overexpressed in aDC after 6 h and 24 h of maturation and 3 miRNAs (miR-17, miR-133b, and miR-203) and miR-23b cluster solely expressed in tDC. We found 5 miRNAs (miR-10a, miR-203, miR-210, miR-30a, and miR-449b) upregulated and 3 miRNAs downregulated (miR-134, miR-145, and miR-149) in both tDC and aDC. These results indicate that miRNAs are specifically modulated in human DC types. This work may contribute to identifying specific modulating miRNAs for aDC and tDC, which could in the future serve as therapeutic targets in the treatment of cancer and autoimmune diseases.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Stumpfova
authors.affiliation: CEITEC, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic ; Masaryk Memorial Cancer Institute, Zlutý Kopec 7, 65653 Brno, Czech Republic.
authors.forename: Renata
authors.initials: R
authors.lastname: Hezova
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic ; INSERM U1046, University of Montpellier I and II, 371 avenue du Doyen G. Giraud, 34295 Montpellier, France.
authors.forename: Albano Carlo
authors.initials: AC
authors.lastname: Meli
authors.affiliation: CEITEC, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic ; Masaryk Memorial Cancer Institute, Zlutý Kopec 7, 65653 Brno, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Slaby
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Michalek
mesh_terms: D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism*; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D008070:Lipopolysaccharides; C547183:MIRN10 microRNA, human; C548234:MIRN133 microRNA, human; C524941:MIRN17 microRNA, human; C546703:MIRN203 microRNA, human; C543800:MIRN210 microRNA, human; C546086:MIRN30b microRNA, human; C544530:MIRN449 microRNA, human; D035683:MicroRNAs; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor