title: Effects of antioxidants of bamboo leaves and flavonoids on 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) formation in chemical model systems.
abstract: The inhibitory effects of antioxidants of bamboo leaves (AOB) and flavonoids against 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) formation were investigated in creatinine and phenylalanine model systems. AOB and the tested flavonoids (orientin, homoorientin, vitexin, isovitex, apigenin, luteolin, isorhamnetin, fisetin, and hesperetin) had significant dose-dependent inhibition effects on PhIP formation with different IC50 values. The superoxide anion (O2(•-)) scavenging activities of these nine flavonoids were evaluated using the pyrogallol autoxidation system. The EC50 values of compounds that showed antioxidant activity were found to correlate well (R(2) = 0.8003) with the corresponding IC50 values representing their inhibition of PhIP formation. It was assumed that the inhibitory effects of flavonoids on PhIP formation were probably achieved by scavenging free radicals generated in the reaction system. These findings provide valuable information for the development of effective strategies to minimize heterocyclic amine content in thermally processed food.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology , Tianjin 300457, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Luo
authors.forename: Zeping
authors.initials: Z
authors.lastname: Shao
authors.forename: Chundi
authors.initials: C
authors.lastname: Yu
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D031723:Bambusa / Q000737:chemistry*; D002273:Carcinogens / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D006358:Hot Temperature; D007093:Imidazoles; D008956:Models, Chemical; D010084:Oxidation-Reduction; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D005419:Flavonoids; D007093:Imidazoles; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine