title: Generation of liver-specific TGF-α and c-Myc-overexpressing fibroblasts for future creation of a liver cancer porcine model.
abstract: Liver cancer is one of the most serious life-threatening diseases in the world. Although the rodent model of hepatocellar carcinoma (HCC) is commonly used, it is limited when considering preclinical applications, including transarterial chemoembolization. The pig is a more appropriate model for applying preclinical procedures as it has similar anatomical and physiological characteristics to humans. In the current study, transgenic fibroblasts were generated that overexpressed two proto-oncogenes specifically in hepatocytes. Porcine TGF-α and c-myc genes were isolated and these were linked with the porcine albumin promoter, which has exhibited selective activity in liver cells. Targeting vectors were introduced into the porcine fibroblasts using a liposome-mediated delivery system and the transgenic cell line was screened with 3 weeks of G-418 treatment. Selected vector‑positive colonies were further confirmed with polymerase chain reaction-based genotyping. Thus, the transgenic cell lines created in the current study should induce liver cancer in pig models following somatic cell nuclear transfer.
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea.
authors.forename: Jeeyoung
authors.initials: J
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea.
authors.forename: Huijeong
authors.initials: H
authors.lastname: Ahn
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea.
authors.forename: Heung-Myong
authors.initials: HM
authors.lastname: Woo
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea.
authors.forename: Eunsong
authors.initials: E
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea.
authors.forename: Geun-Shik
authors.initials: GS
authors.lastname: Lee
mesh_terms: D000418:Albumins / Q000235:genetics; D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D011401:Promoter Regions, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D014162:Transfection; D016211:Transforming Growth Factor alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D000418:Albumins; D016271:Proto-Oncogene Proteins c-myc; D016211:Transforming Growth Factor alpha