title: Effects of acupuncture applied to sanyinjiao with different stimuli on uterine contraction and microcirculation in rats with dysmenorrhea of cold coagulation syndrome.
abstract: In this study, we try to evaluate the effects of acupuncture stimulation with different amounts at Sanyinjiao (SP6) on uterine contraction and uterus microcirculation in rats with dysmenorrhea of cold coagulation syndrome and to explore whether there is direct relativity between "De qi" and needle stimulus intensity. Diestrus female rats were randomly divided into 4 groups, including saline control group, model control group, "A" stimulus group (with strong stimulus), and "B" stimulus group (with weak stimulus). We found that dysmenorrhea rats of the cold coagulation syndrome present a high intensity in uterine tension and high contraction of microvascular diameter. Acupuncture applied with two different stimuli could relieve the symptoms, but, compared with "B" stimulus, "A" stimulus leads to better outcomes on reducing uterine contraction and increasing diameter of uterine microvascular; moreover, hand manipulation during needling mediates the curative effect on the microvascular diameter. Our finding indicates that using thick needles and deep insertion with hand manipulation are more effective and achieve desired level of "De qi" in dysmenorrhea rats.
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Wing-Sze
authors.identifier: 0000-0003-1119-0535
authors.initials: WS
authors.lastname: Hsu
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Shen
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Jia-Min
authors.initials: JM
authors.lastname: Yang
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Li
authors.initials: L
authors.lastname: Luo
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Qi
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Song-Xi
authors.initials: SX
authors.lastname: Shen
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Shi-Peng
authors.initials: SP
authors.lastname: Zhu
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Zhao
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Xiao-Xuan
authors.initials: XX
authors.lastname: Ren
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Meng-Wei
authors.initials: MW
authors.lastname: Guo
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Li
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ji
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Lu-Fen
authors.initials: LF
authors.lastname: Zhang
authors.affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chao Yang District, Beijing 100029, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu