title: Live imaging in Drosophila: The optical and genetic toolkits.
abstract: Biological imaging based on light microscopy comes at the core of the methods that let us understanding morphology and its dynamics in synergy to the spatiotemporal distribution of cellular and molecular activities as the organism develops and becomes functional. Non-linear optical tools and superesolution methodologies are under constant development and their applications to live imaging of whole organisms keep improving as we speak. Genetically coded biosensors, multicolor clonal methods and optogenetics in different organisms and, in particular, in Drosophila follow equivalent paths. We anticipate a brilliant future for live imaging providing the roots for the holistic understanding, rather than for individual parts, of development and function at the whole-organism level.
authors.affiliation: Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Parc Cientific de Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Rebollo
authors.affiliation: Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Parc Cientific de Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain.
authors.forename: Katerina
authors.initials: K
authors.lastname: Karkali
authors.affiliation: Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Parc Cientific de Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain.
authors.forename: Federica
authors.initials: F
authors.lastname: Mangione
authors.affiliation: Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Parc Cientific de Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain. Electronic address: embbmc@ibmb.csic.es.
authors.forename: Enrique
authors.initials: E
authors.lastname: Martín-Blanco
mesh_terms: D000818:Animals; D015509:Developmental Biology / Q000379:methods; D004331:Drosophila melanogaster / Q000235:genetics*; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*
keywords: Biosensors; Clonal analysis; Confocal microscopy; Drosophila; Light sheet fluorescence microscopy; Non-linear optics; Optogenetics; Superesolution