title: NOD1, RIP2 and Caspase12 are potentially novel biomarkers for oral squamous cell carcinoma development and progression.
abstract: Although increasing studies have indicated that Nucleotide-oligomerization domain-containing protein 1 (NOD1) signaling could play an important role in gastrointestinal tumorigenesis, the protein expression and function of NOD1 signaling have not been understood well in oral squamous cell carcinoma (OSCC) progression. The objective of this study is, thus, to examine protein expression of NOD1 signaling immunohistochemically in normal, premalignant and malignant specimens of oral cavity, and to take insights into the association between the protein expression of NOD1 signaling pathway and OSCC precession. In this study immunohistochemical expression of NOD1, Receptor-interacting protein 2 (RIP2), Caspase12, human β Defensin1, 2 and 3 (hBD1, 2, 3) was examined in 15 normal controls, 30 cases of oral leukoplakia (OLK) and 60 cases of OSCC. The immunostaining score was assessed by 2 pathologists, respectively. We found that the expression of NOD1, RIP2, Caspase12, hBD1, 2, and 3 decreased along with the progression of OSCC. NOD1 expression was correlated significantly to tumor differentiation, lymph node metastasis, and tumor size. Our results also showed the correlation of hBD2, 3 to lymph node metastasis of OSCC. These results suggest that the dysfunction of NOD1 signaling pathways could be associated with OSCC development and progression. NOD1, RIP2 and Caspase12 could be used as potentially novel biomarkers for oral carcinogenesis.
authors.affiliation: Department of Oral Medicine & Oral Pathology, Institute and Hospital of Stomatology, Nanjing University Medical School Nanjing 210008, China ; Jiangsu Key Laboratory of Molecular Medicine, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University Nanjing 210093, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Oral Medicine & Oral Pathology, Institute and Hospital of Stomatology, Nanjing University Medical School Nanjing 210008, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Oral Medicine & Oral Pathology, Institute and Hospital of Stomatology, Nanjing University Medical School Nanjing 210008, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Duan
authors.affiliation: Department of Oral Medicine & Oral Pathology, Institute and Hospital of Stomatology, Nanjing University Medical School Nanjing 210008, China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Department of Endodontics, Institute and Hospital of Stomatology, Nanjing University Medical School Nanjing 210008, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Oral Medicine & Oral Pathology, Institute and Hospital of Stomatology, Nanjing University Medical School Nanjing 210008, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Ye
authors.affiliation: Department of Oral Medicine & Oral Pathology, Institute and Hospital of Stomatology, Nanjing University Medical School Nanjing 210008, China.
authors.forename: Hongliu
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Oral Medicine & Oral Pathology, Institute and Hospital of Stomatology, Nanjing University Medical School Nanjing 210008, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Jiangsu Key Laboratory of Molecular Medicine, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University Nanjing 210093, China.
authors.forename: Weiyun
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Oral Medicine & Oral Pathology, Institute and Hospital of Stomatology, Nanjing University Medical School Nanjing 210008, China.
authors.forename: Wenmei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D063646:Carcinogenesis / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D053454:Caspase 12 / Q000235:genetics / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D053474:Nod1 Signaling Adaptor Protein / Q000235:genetics / Q000378:metabolism*; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C406407:DEFB1 protein, human; C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; C119067:NOD1 protein, human; D053474:Nod1 Signaling Adaptor Protein; D023083:beta-Defensins; C506253:RIPK2 protein, human; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2; D053454:Caspase 12
keywords: Caspase12; NOD1; RIP2; human β Defensin; oral squamous cell carcinoma