title: The pyruvate dehydrogenase complexes: structure-based function and regulation.
abstract: The pyruvate dehydrogenase complexes (PDCs) from all known living organisms comprise three principal catalytic components for their mission: E1 and E2 generate acetyl-coenzyme A, whereas the FAD/NAD(+)-dependent E3 performs redox recycling. Here we compare bacterial (Escherichia coli) and human PDCs, as they represent the two major classes of the superfamily of 2-oxo acid dehydrogenase complexes with different assembly of, and interactions among components. The human PDC is subject to inactivation at E1 by serine phosphorylation by four kinases, an inactivation reversed by the action of two phosphatases. Progress in our understanding of these complexes important in metabolism is reviewed.
authors.affiliation: From the Department of Biochemistry, School of Medicine and Biomedical Sciences, University at Buffalo, the State University of New York, Buffalo, New York 14214, mspatel@buffalo.edu.
authors.forename: Mulchand S
authors.initials: MS
authors.lastname: Patel
authors.affiliation: the Department of Chemistry, Rutgers, the State University of New Jersey, Newark, New Jersey 07102.
authors.forename: Natalia S
authors.initials: NS
authors.lastname: Nemeria
authors.affiliation: the Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, and the Veterans Affairs Medical Center, Pittsburgh, Pennsylvania 15240.
authors.forename: William
authors.initials: W
authors.lastname: Furey
authors.affiliation: the Department of Chemistry, Rutgers, the State University of New Jersey, Newark, New Jersey 07102, frjordan@rutgers.edu.
authors.forename: Frank
authors.initials: F
authors.lastname: Jordan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020134:Catalytic Domain; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011768:Pyruvate Dehydrogenase Complex
keywords: Covalent Regulation; Enzyme Catalysis; Protein-Protein Interaction; Pyruvate Dehydrogenase Complex (PDC); Pyruvate Dehydrogenase Kinase (PDC Kinase)