title: Direct label-free electrical immunodetection of transplant rejection protein biomarker in physiological buffer using floating gate AlGaN/GaN high electron mobility transistors.
abstract: Monokine induced by interferon gamma (MIG/CXCL9) is used as an immune biomarker for early monitoring of transplant or allograft rejection. This paper demonstrates a direct electrical, label-free detection method of recombinant human MIG with anti-MIG IgG molecules in physiologically relevant buffer environment. The sensor platform used is a biologically modified GaN-based high electron mobility transistor (HEMT) device. Biomolecular recognition capability was provided by using high affinity anti-MIG monoclonal antibody to form molecular affinity interface receptors on short N-hydroxysuccinimide-ester functionalized disulphide (DSP) self-assembled monolayers (SAMs) on the gold sensing gate of the HEMT device. A floating gate configuration has been adopted to eliminate the influences of external gate voltage. Preliminary test results with the proposed chemically treated GaN HEMT biosensor show that MIG can be detected for a wide range of concentration varying from 5 ng/mL to 500 ng/mL.
authors.forename: Fahmida S
authors.initials: FS
authors.lastname: Tulip
authors.forename: Edward
authors.initials: E
authors.lastname: Eteshola
authors.forename: Suchita
authors.initials: S
authors.lastname: Desai
authors.forename: Salwa
authors.initials: S
authors.lastname: Mostafa
authors.forename: Subramanian
authors.initials: S
authors.lastname: Roopa
authors.forename: Boyd
authors.initials: B
authors.lastname: Evans
authors.forename: Syed Kamrul
authors.initials: SK
authors.lastname: Islam
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques; D002021:Buffers; D054370:Chemokine CXCL9 / Q000032:analysis* / Q000276:immunology; D004583:Electrons; D005708:Gallium / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D014173:Transistors, Electronic
chemical_list: D017607:Aluminum Compounds; D015415:Biomarkers; D002021:Buffers; C080810:CXCL9 protein, human; D054370:Chemokine CXCL9; D007074:Immunoglobulin G; C513700:aluminum gallium nitride; C473348:gallium nitride; D005708:Gallium