title: Macrophage capping protein CapG is a putative oncogene involved in migration and invasiveness in ovarian carcinoma.
abstract: The actin binding protein CapG modulates cell motility by interacting with the cytoskeleton. CapG is associated with tumor progression in different nongynecologic tumor entities and overexpression in breast cancer cell lines correlates with a more invasive phenotype in vitro. Here, we report a significant CapG overexpression in 18/47 (38%) of ovarian carcinomas (OC) analyzed by qRealTime-PCR analyses. Functional analyses in OC cell lines through siRNA mediated CapG knockdown and CapG overexpression showed CapG-dependent cell migration and invasiveness. A single nucleotide polymorphism rs6886 inside the CapG gene was identified, affecting a CapG phosphorylation site and thus potentially modifying CapG function. The minor allele frequency (MAF) of SNP rs6886 (c.1004A/G) was higher and the homozygous (A/A, His335) genotype was significantly more prevalent in patients with fallopian tube carcinomas (50%) as in controls (10%). With OC being one of the most lethal cancer diseases, the detection of novel biomarkers such as CapG could reveal new diagnostic and therapeutic targets. Moreover, in-depth analyses of SNP rs6886 related to FTC and OC will contribute to a better understanding of carcinogenesis and progression of OC.
authors.affiliation: Department of Nephrology, University Hospital Duesseldorf, Heinrich-Heine University, 40225 Duesseldorf, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Glaser
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital Duesseldorf, Heinrich-Heine University, 40225 Duesseldorf, Germany.
authors.forename: M H D
authors.initials: MH
authors.lastname: Neumann
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital Duesseldorf, Heinrich-Heine University, 40225 Duesseldorf, Germany ; Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Qi
authors.identifier: 0000-0001-7977-4962
authors.initials: Q
authors.lastname: Mei
authors.affiliation: Institute of Human Genetics, University Hospital Duesseldorf, Heinrich-Heine University, 40225 Duesseldorf, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Betz
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital Duesseldorf, Heinrich-Heine University, 40225 Duesseldorf, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Seier
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital Duesseldorf, Heinrich-Heine University, 40225 Duesseldorf, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Beyer
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital Duesseldorf, Heinrich-Heine University, 40225 Duesseldorf, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Fehm
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital Duesseldorf, Heinrich-Heine University, 40225 Duesseldorf, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Neubauer
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital Duesseldorf, Heinrich-Heine University, 40225 Duesseldorf, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Niederacher
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital Duesseldorf, Heinrich-Heine University, 40225 Duesseldorf, Germany.
authors.forename: M C
authors.initials: MC
authors.lastname: Fleisch
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D005185:Fallopian Tube Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D008840:Microfilament Proteins; D009687:Nuclear Proteins; D015513:Oncogene Proteins; C066575:CAPG protein, human