title: Immunoprecipitation-based analysis of protein-protein interactions.
abstract: Several techniques allow the detection of protein-protein interactions. In vivo co-immunoprecipitation (Co-IP) studies are an important complement to other commonly used techniques such as yeast two-hybrid or fluorescence complementation, as they reveal interactions between functional proteins at physiological relevant concentrations. Here, we describe an in vivo Co-IP approach using either GFP affinity matrix or specific antibodies to purify proteins of interests and their interacting partners.
authors.affiliation: Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany.
authors.forename: Corinna
authors.initials: C
authors.lastname: Speth
authors.forename: Luis A A
authors.initials: LA
authors.lastname: Toledo-Filho
authors.forename: Sascha
authors.initials: S
authors.lastname: Laubinger
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D057906:Circadian Clocks / Q000502:physiology; D015870:Gene Expression; D017930:Genes, Reporter; D047468:Immunoprecipitation / Q000379:methods*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D011506:Proteins; D011993:Recombinant Fusion Proteins