title: Dynamic changes in saliva after acute mental stress.
abstract: Stress-related variations of fluoride concentration in supernatant saliva and salivary sediment, salivary cortisol, total protein and pH after acute mental stress were assessed. The hypothesis was that stress reactions have no influence on these parameters. Thirty-four male students were distributed into two groups: first received the stress exposure followed by the same protocol two weeks later but without stress exposure, second underwent the protocol without stress exposure followed by the stress exposure two weeks later. The stressor was a public speech followed by tooth brushing. Saliva was collected before, immediately after stress induction and immediately, at 10, 30 and 120 min. after tooth brushing. Cortisol concentrations, total protein, intraoral pH, and fluoride content in saliva were measured. The data were analyzed statistically. Salivary sediment was ca 4.33% by weight of whole unstimulated saliva. Fluoride bioavailability was higher in salivary sediment than in supernatant saliva. The weight and fluoride concentration was not altered during 2 hours after stress exposure. After a public speech, the salivary cortisol concentration significantly increased after 20 minutes compared to the baseline. The salivary protein concentration and pH also increased. Public speaking influences protein concentration and salivary pH but does not alter the fluoride concentration of saliva.
authors.affiliation: Witten/Herdecke University, Faculty of Health, School of Dentistry, Witten, Germany.
authors.forename: Ella A
authors.initials: EA
authors.lastname: Naumova
authors.affiliation: Witten/Herdecke University, Faculty of Health, School of Dentistry, Witten, Germany.
authors.forename: Tudor
authors.initials: T
authors.lastname: Sandulescu
authors.affiliation: Witten/Herdecke University, Faculty of Health, School of Dentistry, Witten, Germany.
authors.forename: Clemens
authors.initials: C
authors.lastname: Bochnig
authors.affiliation: Witten/Herdecke University, Faculty of Health, School of Dentistry, Witten, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Al Khatib
authors.affiliation: Witten/Herdecke University, Faculty of Health, Center for Biomedical Education and Research, Institute for Physiology, Pathophysiology and Toxicology, Witten, Germany.
authors.forename: Wing-Kee
authors.initials: WK
authors.lastname: Lee
authors.affiliation: Witten/Herdecke University, Faculty of Health, School of Dentistry, Witten, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Zimmer
authors.affiliation: Witten/Herdecke University, Faculty of Health, School of Dentistry, Witten, Germany.
authors.forename: Wolfgang H
authors.initials: WH
authors.lastname: Arnold
mesh_terms: D000328:Adult; D001682:Biological Availability; D018592:Cross-Over Studies; D005459:Fluorides / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008297:Male; D012463:Saliva / Q000378:metabolism*; D013315:Stress, Psychological / Q000378:metabolism*; D055815:Young Adult
chemical_list: D005459:Fluorides; D006854:Hydrocortisone