title: Lactobacillus plantarum CLP-0611 ameliorates colitis in mice by polarizing M1 to M2-like macrophages.
abstract: The TNF-α expression-inhibitory effect of lactic acid bacteria (LAB) isolated from kimchi were measured in lipopolysaccharide (LPS)-stimulated peritoneal macrophages. Among the LAB evaluated, Lactobacillus plantarum CLP-0611 inhibited the IL-1β and IL-6 expression, as well as the NF-κB and AP1 activation in LPS-stimulated peritoneal macrophages. Therefore, we investigated its inhibitory effect on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. TNBS significantly induced colon shortening, as well as myeloperoxidase activity and macroscopic score. Oral administration of CLP-0611 significantly reduced TNBS-induced body weight loss, colon shortening, myeloperoxidase activity, IRAK-1 phosphorylation, NF-κB and MAP kinase (p38, ERK, JNK) activation, and iNOS and COX-2 expression. CLP-0611 also inhibited TNBS-induced expression of TNF-α, IL-1β, and IL-6. However, IL-10 expression was induced. CLP-0611 also induced the production of M2 macrophage markers (IL-10, arginase I and CD206). Based on these findings, CLP-0611 inhibits TLR-4-linked NF-κB and MAPK signaling pathways and polarizes M1 to M2-like macrophages, thus ameliorating colitis.
authors.affiliation: Department of Life and Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 130-701, South Korea; Department of Food and Nutrition, Kyung Hee University, Seoul 130-701, South Korea.
authors.forename: Se-Eun
authors.initials: SE
authors.lastname: Jang
authors.affiliation: Department of Food and Nutrition, Kyung Hee University, Seoul 130-701, South Korea.
authors.forename: Myung Joo
authors.initials: MJ
authors.lastname: Han
authors.affiliation: R & D Center, CTOBIO Inc., Gyeonggi-do 445-913, South Korea.
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Life and Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 130-701, South Korea. Electronic address: dhkim@khu.ac.kr.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003092:Colitis / Q000139:chemically induced / Q000276:immunology* / Q000382:microbiology / Q000628:therapy; D003106:Colon / Q000473:pathology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D053592:Interleukin-1 Receptor-Associated Kinases / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D020935:MAP Kinase Signaling System / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000382:microbiology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D019936:Probiotics / Q000008:administration & dosage*; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000008:administration & dosage
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase; D053592:Interleukin-1 Receptor-Associated Kinases
keywords: Colitis; Lactobacillus plantarum CLP-0611; Macrophage; Polarization; TNF-α