title: Characterization of cis- and trans-octadecenoic acid positional isomers in edible fat and oil using gas chromatography-flame ionisation detector equipped with highly polar ionic liquid capillary column.
abstract: In this study, the characterisation of all cis- and trans-octadecenoic acid (C18:1) positional isomers in partially hydrogenated vegetable oil (PHVO) and milk fat, which contain several cis- and trans-C18:1 positional isomers, was achieved by gas chromatography-flame ionisation detector equipped with a highly polar ionic liquid capillary column (SLB-IL111). Prior to analysis, the cis- and trans-C18:1 fractions in PHVO and milk fat were separated using a silver-ion cartridge. The resolution of all cis-C18:1 positional isomers was successfully accomplished at the optimal isothermal column temperature of 120 °C. Similarly, the positional isomers of trans-C18:1, except for trans-6-C18:1 and trans-7-C18:1, were separated at 120 °C. The resolution of trans-6-C18:1 and trans-7-C18:1 isomers was made possible by increasing the column temperature to 160 °C. This analytical method is suitable for determining the cis- and trans-C18:1 positional isomers in edible fats and oils.
authors.affiliation: Tsukishima Foods Industry Co. Ltd., 3-17-9 Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yoshinaga
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Asanuma
authors.affiliation: Tsukishima Foods Industry Co. Ltd., 3-17-9 Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan.
authors.forename: Hoyo
authors.initials: H
authors.lastname: Mizobe
authors.affiliation: Tsukishima Foods Industry Co. Ltd., 3-17-9 Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Kojima
authors.affiliation: Tsukishima Foods Industry Co. Ltd., 3-17-9 Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Nagai
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Beppu
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan. Electronic address: ngotoh@kaiyodai.ac.jp.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Gotoh
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D005223:Fats / Q000032:analysis*; D005410:Flame Ionization; D052578:Ionic Liquids / Q000737:chemistry; D007536:Isomerism; D008892:Milk / Q000737:chemistry; D010938:Plant Oils / Q000032:analysis*; D013229:Stearic Acids / Q000032:analysis*
chemical_list: C114874:5-octadecenoic acid; D005223:Fats; D052578:Ionic Liquids; D010938:Plant Oils; D013229:Stearic Acids
keywords: Gas chromatography; Hydrogenated fat; Ionic liquid; Milk fat; Trans fatty acids