title: Metabolic footprinting: extracellular metabolomic analysis.
abstract: Uptake and excretion of nutrients is an integral part of a cell's physiology. Using analytical chemistry techniques, metabolite uptake and excretion from the culture medium can be quantified. As cellular metabolism changes throughout growth, additional information is available if transient and growth phase-dependent changes are monitored. Here, we describe time-resolved metabolic footprinting (TReF), a technique which employs nuclear magnetic resonance spectroscopy and nonlinear curve fitting to understand and visualize metabolite utilization of P. aeruginosa.
authors.affiliation: Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, UK.
authors.forename: Volker
authors.initials: V
authors.lastname: Behrends
authors.forename: Huw D
authors.initials: HD
authors.lastname: Williams
authors.forename: Jacob G
authors.initials: JG
authors.lastname: Bundy
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013223:Statistics as Topic
chemical_list: D000596:Amino Acids