title: Stable high-titer n-butanol production from sucrose and sugarcane juice by Clostridium acetobutylicum JB200 in repeated batch fermentations.
abstract: The production of n-butanol, a widely used industrial chemical and promising transportation fuel, from abundant, low-cost substrates, such as sugarcane juice, in acetone-butanol-ethanol (ABE) fermentation was studied with Clostridium acetobutylicum JB200, a mutant with high butanol tolerance and capable of producing high-titer (>20 g/L) n-butanol from glucose. Although JB200 is a favorable host for industrial bio-butanol production, its fermentation performance with sucrose and sugarcane juice as substrates has not been well studied. In this study, the long-term n-butanol production from sucrose by JB200 was evaluated with cells immobilized in a fibrous-bed bioreactor (FBB), showing stable performance with high titer (16-20 g/L), yield (∼ 0.21 g/g sucrose) and productivity (∼ 0.32 g/Lh) for 16 consecutive batches over 800 h. Sugarcane thick juice as low-cost substrate was then tested in 3 consecutive batches, which gave similar n-butanol production, demonstrating that JB200 is a robust and promising strain for industrial ABE fermentation.
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave., Columbus, OH 43210, USA.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Jiang
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave., Columbus, OH 43210, USA.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Zhao
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave., Columbus, OH 43210, USA.
authors.forename: Zhongqiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave., Columbus, OH 43210, USA. Electronic address: yang.15@osu.edu.
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
mesh_terms: D020001:1-Butanol / Q000737:chemistry*; D003013:Clostridium / Q000737:chemistry*; D005285:Fermentation; D031786:Saccharum / Q000737:chemistry*; D013395:Sucrose / Q000737:chemistry*
chemical_list: D013395:Sucrose; D020001:1-Butanol
keywords: Acetone–butanol–ethanol fermentation; Butanol; Clostridium acetobutylicum; Fibrous bed bioreactor; Sugarcane juice