title: Design and high-resolution structure of a β³-peptide bundle catalyst.
abstract: Despite the widespread exploration of α-peptides as catalysts, there are few examples of β-peptides that alter the course of a chemical transformation. Our previous work demonstrated that a special class of β(3)-peptides spontaneously self-assembles in water into discrete protein-like bundles possessing unique quaternary structures and exceptional thermodynamic stability. Here we describe a series of β(3)-peptide bundles capable of both substrate binding and chemical catalysis--ester hydrolysis. A combination of kinetic and high-resolution structural analysis suggests an active site triad composed of residues from at least two strands of the octameric bundle structure.
authors.affiliation: Department of Chemistry and ‡Department of Molecular, Cellular and Developmental Biology, Yale University , New Haven, Connecticut 06520-8107, United States.
authors.forename: Pam S P
authors.initials: PS
authors.lastname: Wang
authors.forename: Jennifer B
authors.initials: JB
authors.lastname: Nguyen
authors.forename: Alanna
authors.initials: A
authors.lastname: Schepartz
mesh_terms: D055162:Biocatalysis; D002384:Catalysis; D004950:Esterases / Q000737:chemistry / Q000378:metabolism; D004952:Esters / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D017433:Protein Structure, Secondary
chemical_list: D004952:Esters; D010455:Peptides; D004950:Esterases