title: Spectroscopic studies on the antioxidant activity of ellagic acid.
abstract: Ellagic acid (EA, C14H6O8) is a natural dietary polyphenol whose benefits in a variety of diseases shown in epidemiological and experimental studies involve anti-inflammation, anti-proliferation, anti-angiogenesis, anticarcinogenesis and anti-oxidation properties. In vitro radical scavenging and antioxidant capacity of EA were clarified using different analytical methodologies such as total antioxidant activity determination by ferric thiocyanate, hydrogen peroxide scavenging, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and superoxide anion radical scavenging, ferrous ions (Fe2+) chelating activity and ferric ions (Fe3+) reducing ability. EA inhibited 71.2% lipid peroxidation of a linoleic acid emulsion at 45 μg/mL concentration. On the other hand, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), α-tocopherol and ascorbic acid displayed 69.8%, 66.8%, 64.5% and 59.7% inhibition on the peroxidation of linoleic acid emulsion at the same concentration, respectively. In addition, EA had an effective DPPH• scavenging, ABTS+ scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, ferric ions (Fe3+) reducing power and ferrous ions (Fe2+) chelating activities. Also, those various antioxidant activities were compared to BHA, BHT, α-tocopherol and ascorbic acid as references antioxidant compounds. These results suggested that EA can be used in the pharmacological, food industry and medicine because of these properties.
authors.affiliation: Trakya University, Faculty of Education, Edirne, Turkey. Electronic address: ismailk@trakya.edu.tr.
authors.forename: Ismail
authors.initials: I
authors.lastname: Kilic
authors.affiliation: Trakya University, Faculty of Science, Department of Chemistry, Edirne, Turkey.
authors.forename: Yeşim
authors.initials: Y
authors.lastname: Yeşiloğlu
authors.affiliation: Trakya University, Faculty of Science, Department of Chemistry, Edirne, Turkey.
authors.forename: Yüksel
authors.initials: Y
authors.lastname: Bayrak
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002083:Butylated Hydroxyanisole / Q000737:chemistry; D002084:Butylated Hydroxytoluene / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D004610:Ellagic Acid / Q000737:chemistry*; D004655:Emulsions; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D019787:Linoleic Acid / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D013053:Spectrophotometry; D013481:Superoxides / Q000737:chemistry; D013861:Thiocyanates / Q000737:chemistry; D013997:Time Factors; D024502:alpha-Tocopherol / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002614:Chelating Agents; D004655:Emulsions; D016166:Free Radical Scavengers; D005609:Free Radicals; D008055:Lipids; D010851:Picrates; D013861:Thiocyanates; D013481:Superoxides; D004610:Ellagic Acid; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; C103724:ferric thiocyanate; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron; D024502:alpha-Tocopherol; D001205:Ascorbic Acid; D010100:Oxygen
keywords: Antioxidant activity; Ellagic acid; Free radicals; Metal chelating; Reducing power