title: Antifungal susceptibility testing: current role from the clinical laboratory perspective.
abstract: Despite availability of many antifungal agents, antifungal clinical resistance occurs, perhaps as a consequence of an infecting organism found to be resistant in vitro to one or more antifungals tested. From what derives the important current role of the in vitro antifungal susceptibility testing (AFST), that is to determine which agents are like to be scarcely effective for a given infection. Thus, AFST results, if timely generated by the clinical microbiology laboratory and communicated to clinicians, can aid them in the therapeutic decision making, especially for difficult-to-treat invasive candidiasis and aspergillosis. Although recently refined AFST methods are commercially available for allowing a close antifungal resistance surveillance in many clinical setting, novel assays such as flow cytometry or MALDI-TOF mass spectrometry are upcoming tools for AFST. Based on short-time antifungal drug exposure of fungal isolates, these assays could provide a reliable means for quicker and sensitive assessment of AFST.
authors.affiliation: Institute of Public Health (Section of Hygiene), Università Cattolica del Sacro Cuore, Largo F. Vito, 1-00168, Rome, Italy.
authors.forename: Brunella
authors.initials: B
authors.lastname: Posteraro
authors.affiliation: Institute of Microbiology, Università Cattolica del Sacro Cuore, Largo F. Vito, 1-00168, Rome, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Torelli
authors.affiliation: Institute of Microbiology, Università Cattolica del Sacro Cuore, Largo F. Vito, 1-00168, Rome, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: De Carolis
authors.affiliation: Clinical Laboratory, Ospedale San Carlo, Via Aurelia, 275-00165, Rome, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Posteraro
authors.affiliation: Institute of Microbiology, Università Cattolica del Sacro Cuore, Largo F. Vito, 1-00168, Rome, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Sanguinetti