title: Plasmid mediated kallistain gene expression via intramuscular electroporation delivery in vivo for treatment of NCI-H446 subcutaneous xenograft tumor.
abstract: Kallistatin (KAL) is a novel anti-tumor protein with anti-angiogenic activity. The aim of this study was to investigate whether intramuscular injection of KAL plasmid DNA by electroporation could inhibit NCI-H446 subcutaneous xenograft tumor growth in mice. The tumor model of BALB/c nude mice was induced by subcutaneous inoculation of 5×10(6) NCI-H446 cells into the mice right flank. The next day, naked plasmid pEGFP or pKAL was electrotransfered into the skeletal muscle of nude mice (n=6 for each group), with the optimized electroporation conditions. Tumor cells migration was assessed by E-cadherin staining; Proliferation was determined by anti-Ki-67 staining; and apoptosis was assayed via TUNEL, tumor microvessel density (MVD) was examined by anti-CD34 staining to evaluate the angiogenesis of tumor. Compared to the pEGFP treating group, tumor growth was inhibited by 85% (pEGFP group: 486 ± 187 mm(3), pKAL group: 71±33 mm(3)) at day 42, the MVD of tumor tissues was signiﬁcantly decreased, and tumor cellular proliferation was also inhibited. The results indicate that this therapeutic strategy might serve as a promising approach for cancer clinical therapy.
authors.affiliation: School of Biomedical Science, Institute of Molecular Medicine, Huaqiao University, Quanzhou, China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Jia
authors.affiliation: School of Biomedical Science, Institute of Molecular Medicine, Huaqiao University, Quanzhou, China / Quanzhou Medical College, Quanzhou, China.
authors.forename: Chenna
authors.initials: C
authors.lastname: Zheng
authors.affiliation: School of Biomedical Science, Institute of Molecular Medicine, Huaqiao University, Quanzhou, China.
authors.forename: Jingxian
authors.initials: J
authors.lastname: Feng
authors.affiliation: School of Biomedical Science, Institute of Molecular Medicine, Huaqiao University, Quanzhou, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zou
authors.affiliation: School of Biomedical Science, Institute of Molecular Medicine, Huaqiao University, Quanzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Diao
mesh_terms: D000818:Animals; D018274:Electroporation; D015316:Genetic Therapy; D064593:Heterografts; D006801:Humans; D020287:In Situ Nick-End Labeling; D007273:Injections, Intramuscular; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology / Q000628:therapy*; D010957:Plasmids; D015843:Serpins / Q000235:genetics*
chemical_list: D015843:Serpins; C051324:kallistatin