title: Bacterial leakage in root canals filled with AH Plus and dentine bonding agents.
abstract: OBJECTIVE
  The aim of this study was to compare the efficacy of different dentine adhesives in delaying the coronal bacterial leakage of Enterococcus faecalis in filled root canals. Materials and methods. Ninety-five lower incisors of patients >65 years of age were instrumented using the ProTaper system and were irrigated with 1 mL of 2.5% sodium hypochlorite (NaOCl) alternated with 1 mL 17% EDTA between each file change. Final irrigation was performed with 5 mL of 17% EDTA and then flushed with 5 mL of distilled water. The teeth were randomly divided into five experimental groups (n = 15/group) and one of the following dentine adhesives was applied: (1) AdheSE; (2) Excite DSC; (3) Clearfil Protect Bond; (4) One Coat 7.0; or (5) Control group without adhesive. After filling the root canals, the samples were mounted on a double chamber device to evaluate the bacterial filtration of E. faecalis during a period of 240 days. The results underwent non-parametric Kaplan-Meier survival analysis and comparisons among groups were done using the Log-Rank test.
  
  
  RESULTS
  At 240 days, E. faecalis was detected in samples of all groups in the lower chamber. The highest survival value was obtained by One Coat 7.0, giving statistically significant differences from the other groups, whereas Clearfil Protect Bond, AdheSE and Excite DSC showed similar behaviours, likewise similar to the Control group.
  
  
  CONCLUSIONS
  One Coat 7.0 adhesive system provides the longest survival value to delay E. faecalis coronal leakage in filled root canals.
authors.affiliation: Department of Stomatology.
authors.forename: Esther
authors.initials: E
authors.lastname: Navarro-Escobar
authors.forename: Pilar
authors.initials: P
authors.lastname: Baca
authors.forename: Matilde
authors.initials: M
authors.lastname: Ruiz-Linares
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Arias-Moliz
authors.forename: Mercedes
authors.initials: M
authors.lastname: Perez-Heredia
authors.forename: Carmen Maria
authors.initials: CM
authors.lastname: Ferrer-Luque
mesh_terms: D000180:Acrylic Resins / Q000627:therapeutic use; D000368:Aged; D003763:Dental Leakage / Q000382:microbiology*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D003804:Dentin / Q000382:microbiology; D017220:Dentin-Bonding Agents / Q000737:chemistry* / Q000627:therapeutic use; D004492:Edetic Acid / Q000627:therapeutic use; D013293:Enterococcus faecalis / Q000502:physiology*; D004853:Epoxy Resins / Q000627:therapeutic use*; D006171:Gutta-Percha / Q000627:therapeutic use; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000627:therapeutic use; D011897:Random Allocation; D019279:Resin Cements / Q000627:therapeutic use; D012387:Root Canal Filling Materials / Q000627:therapeutic use*; D012388:Root Canal Irrigants / Q000627:therapeutic use; D018915:Root Canal Preparation / Q000295:instrumentation; D012973:Sodium Hypochlorite / Q000627:therapeutic use
chemical_list: D000180:Acrylic Resins; C489662:AdheSE cement; C503075:Clearfil Protect Bond; D017220:Dentin-Bonding Agents; D004853:Epoxy Resins; C420130:Excite cement; D008689:Methacrylates; C426984:One Coat Bond; D019279:Resin Cements; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; C534916:epoxy resin-based root canal sealer; D006171:Gutta-Percha; D004492:Edetic Acid; D012973:Sodium Hypochlorite
keywords: Enterococcus faecalis; adhesive systems; antibacterial agents; leakage; root canal filling