title: Adjuvant effect of polysaccharide from fruits of Physalis alkekengi L. in DNA vaccine against systemic candidiasis.
abstract: Adjuvant effect mediated by polysaccharide (PPSB) isolated from the fruits of Physalis alkekengi L. in DNA vaccine was evaluated in mice. Recombinant plasmid containing epitope C (LKVIRK) from heat shock protein 90 (HSP90) of Candida albicans (C. albican) was used as DNA vaccine (pD-HSP90C). The results indicated that PPSB significantly enhanced specific antibody titers IgG, IgG1, IgG2b, and concentration of IL-2 and IL-4 in sera of mice immunized with pD-HSP90C (p<0.05). More importantly, it was found that the mice immunized with pD-HSP90C/PPSB not only had fewer CFU (colony forming unites) in the kidneys than mice immunized with pD-HSP90C, but also a statistically significant higher survival rate over PBS-injected group (p<0.05) when the immunized mice were challenged with living C. albican cells. However, no statistically significant difference in survival rate was observed between pD-HSP90C-immunized group and PBS-injected group. Therefore, PPSB can be considered as a promising adjuvant eliciting both Th1 and Th2 responses to enhance the efficacy of DNA vaccines.
authors.affiliation: School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun City, Jilin Province 130024, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun City, Jilin Province 130024, China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Han
authors.affiliation: School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun City, Jilin Province 130024, China.
authors.forename: Danyang
authors.initials: D
authors.lastname: Zhao
authors.affiliation: School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun City, Jilin Province 130024, China. Electronic address: wanggy737@nenu.edu.cn.
authors.forename: Guiyun
authors.initials: G
authors.lastname: Wang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D002176:Candida albicans / Q000276:immunology; D002177:Candidiasis / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D016207:Cytokines / Q000097:blood; D004353:Drug Evaluation, Preclinical; D005638:Fruit / Q000737:chemistry*; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology; D016469:Fungemia / Q000517:prevention & control; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology; D007668:Kidney / Q000382:microbiology; D008297:Male; D008813:Mice, Inbred ICR; D032321:Physalis / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage*; D011134:Polysaccharides / Q000008:administration & dosage*; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage*
chemical_list: D000276:Adjuvants, Immunologic; D000908:Antibodies, Fungal; D016207:Cytokines; D005656:Fungal Proteins; D018841:HSP90 Heat-Shock Proteins; D010936:Plant Extracts; D011134:Polysaccharides; D019444:Vaccines, DNA
keywords: Adjuvant; Candida albicans; DNA vaccine; Physalis alkekengi L. polysaccharide