title: Calorie restriction-induced changes in the secretome of human adipocytes, comparison with resveratrol-induced secretome effects.
abstract: Obesity is characterized by dysfunctional white adipose tissue (WAT) that ultimately may lead to metabolic diseases. Calorie restriction (CR) reduces the risk for age and obesity-associated complications. The impact of CR on obesity has been examined with human intervention studies, which showed alterations in circulating adipokines. However, a direct effect of CR on the human adipocyte secretome remains elusive. Therefore, the effect of a 96h low glucose CR on the secretion profile of in vitro cultured mature human SGBS adipocytes was investigated by using proteomics technology. Low-glucose CR decreased the adipocyte triglyceride contents and resulted in an altered secretion profile. Changes in the secretome indicated an improved inflammatory phenotype. In addition, several adipocyte-secreted proteins related to insulin resistance showed a reversed expression after low-glucose CR. Furthermore, 6 novel CR-regulated adipocyte-secreted proteins were identified. Since resveratrol (RSV) mimics CR we compared results from this study with data from our previous RSV study on the SGBS adipocyte secretome. The CR and RSV adipocyte secretomes partly differed from each other, although both treatment strategies lead to secretome changes indicating a less inflammatory phenotype. Furthermore, both treatments induced SIRT1 expression and resulted in a reversed expression of detrimental adipokines associated with metabolic complications.
authors.affiliation: NUTRIM School for Nutrition, Toxicology and Metabolism, Department of Human Biology, Maastricht University, Maastricht, The Netherlands. Electronic address: j.renes@maastrichtuniversity.nl.
authors.forename: Johan
authors.initials: J
authors.lastname: Renes
authors.affiliation: NUTRIM School for Nutrition, Toxicology and Metabolism, Department of Human Biology, Maastricht University, Maastricht, The Netherlands.
authors.forename: Anja
authors.initials: A
authors.lastname: Rosenow
authors.affiliation: NUTRIM School for Nutrition, Toxicology and Metabolism, Department of Human Biology, Maastricht University, Maastricht, The Netherlands.
authors.forename: Nadia
authors.initials: N
authors.lastname: Roumans
authors.affiliation: Biomedical Research Institute, Hasselt University, and School of Life Sciences, Transnationale Universiteit Limburg, Diepenbeek, Belgium.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Noben
authors.affiliation: NUTRIM School for Nutrition, Toxicology and Metabolism, Department of Human Biology, Maastricht University, Maastricht, The Netherlands.
authors.forename: Edwin C M
authors.initials: EC
authors.lastname: Mariman
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D054392:Adipokines / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D052436:Adipose Tissue, White / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000975:Antioxidants / Q000494:pharmacology*; D001145:Arrhythmias, Cardiac / Q000378:metabolism / Q000473:pathology; D031204:Caloric Restriction; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000187:drug effects; D040181:Genetic Diseases, X-Linked / Q000378:metabolism / Q000473:pathology; D005877:Gigantism / Q000378:metabolism / Q000473:pathology; D005947:Glucose / Q000172:deficiency; D006330:Heart Defects, Congenital / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007333:Insulin Resistance; D008607:Intellectual Disability / Q000378:metabolism / Q000473:pathology; D058977:Molecular Sequence Annotation; D009765:Obesity / Q000378:metabolism / Q000473:pathology; D020543:Proteome / Q000302:isolation & purification* / Q000378:metabolism; D040901:Proteomics; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry
chemical_list: D054392:Adipokines; D000975:Antioxidants; D020543:Proteome; D013267:Stilbenes; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D005947:Glucose; D000077185:Resveratrol
keywords: 2-DE LC–MS/MS; Adipokines; Calorie restriction; Human adipocytes; Metabolic syndrome