title: [Enhancement of in vitro protoscolicidal effects of high-intensity focused ultrasound by a superabsorbent polymer and ultrasound contrast agent].
abstract: This study evaluated whether or not a superabsorbent polymer (SAP) combined with ultrasound contrast agent (UCA) could enhance damage efficacy of high intensity focused ultrasound (HIFU) on Echinococcus granulosus protoscoleces in vitro. Thirty test tubes each with 6 000-7 500 protoscolices were divided into 5 groups: group A (blank control) without HIFU treatment, group B treated with HIFU (50 W) only, group C treated with 10 microl UCA and HIFU, group D treated with 0.01 g SAP and HIFU, group E treated with 10 microl UCA, 0.01 g SAP, and HIFU. In group B, echo enhancement of ultrasound image, suspension temperature (26.0 degrees C +/- 0.2 degrees C) and protoscoleces mortality (30.4%) were higher than that of group A (18.0 degrees C +/- 0.1 degrees C, 1.9%) (P < 0.01). Compared with group B, the echo enhancement of ultrasound image, suspension temperature (27.0 degrees C +/- 0.2 degrees C, 28.2 degrees C +/- 0.2 degrees C) and protoscoleces mortality (50.0%, 53.7%) of groups C and D increased significantly (P < 0.01). In group E, more protoscoleces were stained in red and their internal structures were indistinct. By chi-square test, the protoscoleces mortality of group E (69.7%) was higher than that of groups C and D (P < 0.01). There was no significant difference in suspension temperature among the 3 groups.
authors.forename: Hui
authors.initials: H
authors.lastname: Cai
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Ye
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Zhao
authors.forename: Ai-Bo
authors.initials: AB
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Zhao
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000494:pharmacology*; D048209:Echinococcus granulosus / Q000187:drug effects*; D011108:Polymers / Q000494:pharmacology*; D014465:Ultrasonics
chemical_list: D003287:Contrast Media; D011108:Polymers