title: The environmental biological signature: NGS profiling for forensic comparison of soils.
abstract: The identification of the source of a specific soil sample is a crucial step in forensic investigations. Rapid advances in next generation sequencing (NGS) technology and the strong reduction of the cost of sequencing have recently opened new perspectives. In the present work a metabarcoding approach has been successfully applied to forensic and environmental soil samples, allowing the accurate and sensitive analysis of microflora (mfDNA), plants, metazoa, and protozoa DNA. The identification of the biological component by DNA metabarcoding is a strong element for the discrimination of samples geologically very similar but coming for distinct environments.
authors.affiliation: University of Rome "Foro Italico", Department of Movement, Human and Health Sciences, Public Health Unit, Piazza Lauro De Bosis, 6, 00135 Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Giampaoli
authors.affiliation: Carabinieri, Reparto Investigazioni Scientifiche di Roma, Viale di Tor di Quinto, 119, 00191 Roma, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Berti
authors.affiliation: Geoscienze Forensi Italia(®), Viale Mediterraneo 77, 00122 Rome, Italy.
authors.forename: R M
authors.initials: RM
authors.lastname: Di Maggio
authors.affiliation: Department of Biology, University of Florence, Via del Proconsolo 12, 50122 Florence, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Pilli
authors.affiliation: Spygen, Savoie Technolac, Bâtiment Koala, 17, rue du Lac Saint-André, BP 274, 73375 Le Bourget-du-Lac Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Valentini
authors.affiliation: University of Rome "Foro Italico", Department of Movement, Human and Health Sciences, Public Health Unit, Piazza Lauro De Bosis, 6, 00135 Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Valeriani
authors.affiliation: University of Rome "Foro Italico", Department of Movement, Human and Health Sciences, Public Health Unit, Piazza Lauro De Bosis, 6, 00135 Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Gianfranceschi
authors.affiliation: Carabinieri, Reparto Investigazioni Scientifiche di Roma, Viale di Tor di Quinto, 119, 00191 Roma, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Barni
authors.affiliation: Carabinieri, Reparto Investigazioni Scientifiche di Roma, Viale di Tor di Quinto, 119, 00191 Roma, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Ripani
authors.affiliation: University of Rome "Foro Italico", Department of Movement, Human and Health Sciences, Public Health Unit, Piazza Lauro De Bosis, 6, 00135 Rome, Italy; MDD University Spin off, Viterbo, Italy. Electronic address: vincenzo.romanospica@uniroma4.it.
authors.forename: V
authors.initials: V
authors.lastname: Romano Spica
mesh_terms: D000818:Animals; D004269:DNA, Bacterial; D018744:DNA, Plant; D044707:Forensic Sciences; D016678:Genome; D006801:Humans; D008903:Minerals / Q000032:analysis; D017422:Sequence Analysis, DNA / Q000379:methods*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; D008903:Minerals; D012987:Soil
keywords: Metabarcoding; Soil; mFDNA