title: The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM).
abstract: The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.
authors.affiliation: Department of Biochemistry, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, 14049-900, Ribeirao Preto, São Paulo, Brazil, vitor.faca@fmrp.usp.br.
authors.forename: Vitor M
authors.initials: VM
authors.lastname: Faça
authors.forename: Camila S
authors.initials: CS
authors.lastname: Palma
authors.forename: Daniele
authors.initials: D
authors.lastname: Albuquerque
authors.forename: Gabriela N S
authors.initials: GN
authors.lastname: Canchaya
authors.forename: Mariana L
authors.initials: ML
authors.lastname: Grassi
authors.forename: Vera L
authors.initials: VL
authors.lastname: Epifânio
authors.forename: Eduardo B
authors.initials: EB
authors.lastname: de Oliveira
mesh_terms: D045744:Cell Line, Tumor; D003470:Culture Media; D057166:High-Throughput Screening Assays; D006801:Humans; D013058:Mass Spectrometry; D040901:Proteomics
chemical_list: D003470:Culture Media