title: Quantification of homoegonol in rat plasma using liquid chromatography-tandem mass spectrometry and its pharmacokinetics application.
abstract: Homoegonol is a biologically active neolignan isolated from Styrax species with cytotoxic, antimicrobial, anti-inflammatory and anti-asthma activities. For the quantification of homoegonol in rat plasma, a selective and sensitive liquid chromatography-tandem mass spectrometric method was developed and validated for the first time using protein precipitation with methanol as a sample clean-up procedure. The analytes were separated in an Atlantis dC18 column using a gradient elution of methanol and 0.1% formic acid, and mass-to-charge ratios were determined in selective reaction monitoring mode using tandem mass spectrometry with m/z 343.12 > 296.97 for homoegonol and m/z 517.30 > 282.90 for udenafil (internal standard). The standard curve was linear over the concentration ranges of 1 - 500 ng/mL using a 30 μL rat plasma sample. The coefficient of variation and relative error for intra- and inter-assay at four quality control levels were 3.9-10.0 and -3.3-2.7%, respectively. The overall recovery of homoegonol from rat plasma using protein precipitation was 99.7 ± 7.7%. The pharmacokinetics parameters of homoegonol were dose-independent after both intravenous (1, 2.5 and 5 mg/kg doses) and oral (5, 10 and 20 mg/kg doses) administration in male Sprague-Dawley rats.
authors.affiliation: Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, The Catholic University of Korea, Bucheon, 420-743, Republic of Korea.
authors.forename: Hyeon-Uk
authors.initials: HU
authors.lastname: Jeong
authors.forename: Soon Sang
authors.initials: SS
authors.lastname: Kwon
authors.forename: Deok-Gyu
authors.initials: DG
authors.lastname: Hwang
authors.forename: Eun Nam
authors.initials: EN
authors.lastname: Kim
authors.forename: Kyeong
authors.initials: K
authors.lastname: Lee
authors.forename: Kyung-Seop
authors.initials: KS
authors.lastname: Ahn
authors.forename: Sei-Ryang
authors.initials: SR
authors.lastname: Oh
authors.forename: Hye Suk
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D017705:Lignans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D032362:Styrax; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D017705:Lignans
keywords: homoegonol; liquid chromatography-tandem mass spectrometry; pharmacokinetics; rat plasma