title: Characterization of the cell surface properties of drinking water pathogens by microbial adhesion to hydrocarbon and electrophoretic mobility measurements.
abstract: The surface characteristics of microbial cells directly influence their mobility and behavior within aqueous environments. The cell surface hydrophobicity (CSH) and electrophoretic mobility (EPM) of microbial cells impact a number of interactions and processes including aggregation, adhesion to surfaces, and stability of the cells within the aqueous environments. These cell characteristics are unique to the bacterial species and are a reflection of the large diversity of surface structures, proteins, and appendages of microorganisms. CSH and EPM of bacterial cells contribute substantially to the effectiveness of drinking water treatment to remove them, and therefore an investigation of these properties will be useful in predicting their removal through drinking water treatment processes and transport through drinking water distribution systems. EPM and CSH measurements of six microbiological pathogen or surrogate species suspended in phosphate-buffered water are reported in this work. Two strains of Vibrio cholerae were hydrophobic, while three strains of Escherichia coli were hydrophilic. Bacillus cereus was categorized as moderately hydrophobic. The strains of E. coli had the highest (most negative) EPM. Based on the measurements, E. coli species is predicted to be most difficult to remove from water while V. cholerae will be the easiest to remove.
authors.affiliation: Department of Biological Sciences, University of Cincinnati, 2100 Clifton Ave., Cincinnati, OH 45220, United States. Electronic address: popovici.jonathan@epa.gov.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Popovici
authors.affiliation: Pegasus Technical Services Inc., 46 E. Hollister St., Cincinnati, OH 45219, United States. Electronic address: white.colin@epa.gov.
authors.forename: Colin P
authors.initials: CP
authors.lastname: White
authors.affiliation: U.S. Environmental Protection Agency, ORD, NMRML, WSWRD, 26 W. Martin Luther King Dr., Cincinnati, OH 45268, United States.
authors.forename: Jill
authors.initials: J
authors.lastname: Hoelle
authors.affiliation: Department of Biological Sciences, University of Cincinnati, 2100 Clifton Ave., Cincinnati, OH 45220, United States.
authors.forename: Brian K
authors.initials: BK
authors.lastname: Kinkle
authors.affiliation: U.S. Environmental Protection Agency, ORD, NMRML, WSWRD, 26 W. Martin Luther King Dr., Cincinnati, OH 45268, United States. Electronic address: lytle.darren@epa.gov.
authors.forename: Darren A
authors.initials: DA
authors.lastname: Lytle
mesh_terms: D001409:Bacillus cereus / Q000187:drug effects; D001419:Bacteria / Q000166:cytology* / Q000187:drug effects; D001422:Bacterial Adhesion / Q000187:drug effects*; D002021:Buffers; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects; D060766:Drinking Water / Q000382:microbiology*; D004586:Electrophoresis; D004926:Escherichia coli / Q000187:drug effects; D006838:Hydrocarbons / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D013499:Surface Properties; D014734:Vibrio cholerae / Q000187:drug effects
chemical_list: D002021:Buffers; D060766:Drinking Water; D006838:Hydrocarbons
keywords: Drinking water; Electrophoretic mobility; Hydrophobicity; Pathogen; Surface