title: Partitioning of polychlorinated biphenyls into human cells and adipose tissues: evaluation of octanol, triolein, and liposomes as surrogates.
abstract: Whereas octanol, triacylglycerides, and liposomes have all been proposed as surrogates for measuring the affinity of hydrophobic organic contaminants to human lipids, no comparative evaluation of their suitability exists. Here we conducted batch sorption experiments with polyoxymethylene passive samplers to determine the partition coefficients at 37 °C of 18 polychlorinated biphenyls (PCBs) from water into (i) triolein (Ktriolein/water), (ii) eight types of liposomes (Kliposome/water), (iii) human abdominal fat tissues (KAFT/water) from seven individuals, and (iv) human MCF-7 cells cultured in vitro (Kcell/water). Differences between KAFT/water among individuals and between Kliposome/water among liposome types were very small and not correlated to structural attributes of the PCBs. Similarly, the length and degree of saturation of the phospholipid carbon chains, the headgroup, and the composition of the liposome did not affect the partitioning of PCBs into the studied liposomes. Whereas Kliposome/water values were similar to literature values of Koctanol/water adjusted to 37 °C, they both were lower than KAFT/water and Kcell/water by a factor of 3 on average. Partitioning of PCBs into triolein on the other hand closely mimicked that into human lipids, for which triolein is thus a better surrogate than either octanol or liposomes. Previously published polyparameter linear free energy relationships for partitioning from water into storage lipids and liposomes predicted the measured partition coefficients with a root-mean-square error of less than 0.15 log units, if the chosen equations and solute descriptors do not allow chlorine substitution in the ortho-position to influence the prediction. By guiding the selection of (i) a surrogate for the experimental determination and (ii) a method for the prediction of partitioning into human lipids, this study contributes to a better assessment of hydrophobic organic contaminant bioaccumulation in humans.
authors.affiliation: Institute for Risk Assessment Sciences, Utrecht University , P.O. Box 80177, 3508 TD Utrecht, The Netherlands.
authors.forename: Cristina L
authors.initials: CL
authors.lastname: Quinn
authors.forename: Stephan A
authors.initials: SA
authors.lastname: van der Heijden
authors.forename: Frank
authors.initials: F
authors.lastname: Wania
authors.forename: Michiel T O
authors.initials: MT
authors.lastname: Jonker
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D000368:Aged; D002713:Chlorine / Q000378:metabolism; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D061986:MCF-7 Cells; D008875:Middle Aged; D000442:Octanols / Q000737:chemistry / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000737:chemistry / Q000378:metabolism*; D013816:Thermodynamics; D014304:Triolein / Q000737:chemistry / Q000378:metabolism*; D014867:Water / Q000737:chemistry
chemical_list: D008055:Lipids; D008081:Liposomes; D000442:Octanols; D014867:Water; D014304:Triolein; D002713:Chlorine; D011078:Polychlorinated Biphenyls