title: Amniotic fluid-derived mesenchymal stem cells prevent fibrosis and preserve renal function in a preclinical porcine model of kidney transplantation.
abstract: It is well known that ischemia/reperfusion injuries strongly affect the success of human organ transplantation. Development of interstitial fibrosis and tubular atrophy is the main deleterious phenomenon involved. Stem cells are a promising therapeutic tool already validated in various ischemic diseases. Amniotic fluid-derived mesenchymal stem cells (af-MSCs), a subpopulation of multipotent cells identified in amniotic fluid, are known to secrete growth factors and anti-inflammatory cytokines. In addition, these cells are easy to collect, present higher proliferation and self-renewal rates compared with other adult stem cells (ASCs), and are suitable for banking. Consequently, af-MSCs represent a promising source of stem cells for regenerative therapies in humans. To determine the efficiency and the safety of af-MSC infusion in a preclinical porcine model of renal autotransplantation, we injected autologous af-MSCs in the renal artery 6 days after transplantation. The af-MSC injection improved glomerular and tubular functions, leading to full renal function recovery and abrogated fibrosis development at 3 months. The strong proof of concept generated by this translational porcine model is a first step toward evaluation of af-MSC-based therapies in human kidney transplantation.
authors.affiliation: INSERM U1082, Université de Poitiers, Faculté de Médecine et de Pharmacie, Poitiers, France; CHU de Poitiers, Poitiers, France; INSERM U935, Poitiers and Villejuif, France; INSERM U935, Esteam Pluripotent Stem Cell Core Facility and Ingestem Infrastructure, Université Paris Sud XI, Villejuif, France; INRA, UE1372 GenESI, Plateforme Ibisa, Surgères, France.
authors.forename: Edouard
authors.initials: E
authors.lastname: Baulier
authors.affiliation: INSERM U1082, Université de Poitiers, Faculté de Médecine et de Pharmacie, Poitiers, France; CHU de Poitiers, Poitiers, France; INSERM U935, Poitiers and Villejuif, France; INSERM U935, Esteam Pluripotent Stem Cell Core Facility and Ingestem Infrastructure, Université Paris Sud XI, Villejuif, France; INRA, UE1372 GenESI, Plateforme Ibisa, Surgères, France.
authors.forename: Frederic
authors.initials: F
authors.lastname: Favreau
authors.affiliation: INSERM U1082, Université de Poitiers, Faculté de Médecine et de Pharmacie, Poitiers, France; CHU de Poitiers, Poitiers, France; INSERM U935, Poitiers and Villejuif, France; INSERM U935, Esteam Pluripotent Stem Cell Core Facility and Ingestem Infrastructure, Université Paris Sud XI, Villejuif, France; INRA, UE1372 GenESI, Plateforme Ibisa, Surgères, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Le Corf
authors.affiliation: INSERM U1082, Université de Poitiers, Faculté de Médecine et de Pharmacie, Poitiers, France; CHU de Poitiers, Poitiers, France; INSERM U935, Poitiers and Villejuif, France; INSERM U935, Esteam Pluripotent Stem Cell Core Facility and Ingestem Infrastructure, Université Paris Sud XI, Villejuif, France; INRA, UE1372 GenESI, Plateforme Ibisa, Surgères, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Jayle
authors.affiliation: INSERM U1082, Université de Poitiers, Faculté de Médecine et de Pharmacie, Poitiers, France; CHU de Poitiers, Poitiers, France; INSERM U935, Poitiers and Villejuif, France; INSERM U935, Esteam Pluripotent Stem Cell Core Facility and Ingestem Infrastructure, Université Paris Sud XI, Villejuif, France; INRA, UE1372 GenESI, Plateforme Ibisa, Surgères, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Schneider
authors.affiliation: INSERM U1082, Université de Poitiers, Faculté de Médecine et de Pharmacie, Poitiers, France; CHU de Poitiers, Poitiers, France; INSERM U935, Poitiers and Villejuif, France; INSERM U935, Esteam Pluripotent Stem Cell Core Facility and Ingestem Infrastructure, Université Paris Sud XI, Villejuif, France; INRA, UE1372 GenESI, Plateforme Ibisa, Surgères, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Goujon
authors.affiliation: INSERM U1082, Université de Poitiers, Faculté de Médecine et de Pharmacie, Poitiers, France; CHU de Poitiers, Poitiers, France; INSERM U935, Poitiers and Villejuif, France; INSERM U935, Esteam Pluripotent Stem Cell Core Facility and Ingestem Infrastructure, Université Paris Sud XI, Villejuif, France; INRA, UE1372 GenESI, Plateforme Ibisa, Surgères, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Feraud
authors.affiliation: INSERM U1082, Université de Poitiers, Faculté de Médecine et de Pharmacie, Poitiers, France; CHU de Poitiers, Poitiers, France; INSERM U935, Poitiers and Villejuif, France; INSERM U935, Esteam Pluripotent Stem Cell Core Facility and Ingestem Infrastructure, Université Paris Sud XI, Villejuif, France; INRA, UE1372 GenESI, Plateforme Ibisa, Surgères, France.
authors.forename: Annelise
authors.initials: A
authors.lastname: Bennaceur-Griscelli
authors.affiliation: INSERM U1082, Université de Poitiers, Faculté de Médecine et de Pharmacie, Poitiers, France; CHU de Poitiers, Poitiers, France; INSERM U935, Poitiers and Villejuif, France; INSERM U935, Esteam Pluripotent Stem Cell Core Facility and Ingestem Infrastructure, Université Paris Sud XI, Villejuif, France; INRA, UE1372 GenESI, Plateforme Ibisa, Surgères, France t.hauet@chu-poitiers.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Hauet
authors.affiliation: INSERM U1082, Université de Poitiers, Faculté de Médecine et de Pharmacie, Poitiers, France; CHU de Poitiers, Poitiers, France; INSERM U935, Poitiers and Villejuif, France; INSERM U935, Esteam Pluripotent Stem Cell Core Facility and Ingestem Infrastructure, Université Paris Sud XI, Villejuif, France; INRA, UE1372 GenESI, Plateforme Ibisa, Surgères, France.
authors.forename: Ali G
authors.initials: AG
authors.lastname: Turhan
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000818:Animals; D058948:Cell Tracking; D002478:Cells, Cultured; D005260:Female; D005355:Fibrosis; D006085:Graft Survival; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D007668:Kidney / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D016030:Kidney Transplantation / Q000009:adverse effects*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000378:metabolism / Q000637:transplantation*; D011247:Pregnancy; D020127:Recovery of Function; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D013552:Swine; D013997:Time Factors; D014161:Transduction, Genetic; D014162:Transfection / Q000379:methods; D014182:Transplantation, Autologous
chemical_list: D049452:Green Fluorescent Proteins
keywords: Amniotic fluid-derived mesenchymal stem cells; Deceased after cardiac arrest donors; Ischemia reperfusion injury; Kidney transplantation; Pig; Preclinical model