title: Assembly and nuclear export of pre-ribosomal particles in budding yeast.
abstract: The ribosome is responsible for the final step of decoding genetic information into proteins. Therefore, correct assembly of ribosomes is a fundamental task for all living cells. In eukaryotes, the construction of the ribosome which begins in the nucleolus requires coordinated efforts of >350 specialized factors that associate with pre-ribosomal particles at distinct stages to perform specific assembly steps. On their way through the nucleus, diverse energy-consuming enzymes are thought to release assembly factors from maturing pre-ribosomal particles after accomplishing their task(s). Subsequently, recruitment of export factors prepares pre-ribosomal particles for transport through nuclear pore complexes. Pre-ribosomes are exported into the cytoplasm in a functionally inactive state, where they undergo final maturation before initiating translation. Accumulating evidence indicates a tight coupling between nuclear export, cytoplasmic maturation, and final proofreading of the ribosome. In this review, we summarize our current understanding of nuclear export of pre-ribosomal subunits and cytoplasmic maturation steps that render pre-ribosomal subunits translation-competent.
authors.affiliation: Institute of Biochemistry (IBC), ETH Zürich, Otto-Stern-Weg 3, 8093, Zürich, Switzerland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Gerhardy
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Menet
authors.forename: Cohue
authors.initials: C
authors.lastname: Peña
authors.forename: Janusz Jurand
authors.initials: JJ
authors.lastname: Petkowski
authors.forename: Vikram Govind
authors.initials: VG
authors.lastname: Panse
mesh_terms: D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism*; D008958:Models, Molecular; D054657:Ribosome Subunits / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*