title: Chemotypes sensitivity and predictivity of in vivo outcomes for cytotoxic assays in THLE and HepG2 cell lines.
abstract: In the present study, we demonstrate the utility of in vitro ATP depletion assays in both THLE and HepG2 cells for predicting the toxicological outcome in Exploratory Toxicology Studies across 446 Pfizer proprietary compounds. Our results suggest a higher likelihood of selecting suitable compounds for in vivo safety studies by using cytotoxicity assays in multiple cell-lines over a single cell line. In addition, we demonstrate that different cell-lines have different sensitivities to compounds depending on their ionization state, that is, acid, base or neutral. HepG2 cells are more sensitive for basic compounds, whereas THLE cells have a relatively higher sensitivity for the acidic and neutral compounds. These in vitro cytotoxicity assays when combined with physicochemical properties (cLogP >3 and topological polar surface area (TPSA) <75Å(2)), are the most effective means to prioritize compounds having a lower probability of causing adverse events in vivo.
authors.affiliation: Compound Safety Prediction, Pfizer Global Research & Development, Groton, CT, United States. Electronic address: Falgun.shah@pfizer.com.
authors.forename: Falgun
authors.initials: F
authors.lastname: Shah
authors.affiliation: Compound Safety Prediction, Pfizer Global Research & Development, Groton, CT, United States.
authors.forename: Shirley
authors.initials: S
authors.lastname: Louise-May
authors.affiliation: Compound Safety Prediction, Pfizer Global Research & Development, Groton, CT, United States.
authors.forename: Nigel
authors.initials: N
authors.lastname: Greene
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D002460:Cell Line; D003603:Cytotoxins / Q000737:chemistry / Q000633:toxicity*; D004354:Drug Screening Assays, Antitumor; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D012372:ROC Curve; D018675:Toxicity Tests
chemical_list: D003603:Cytotoxins; D000255:Adenosine Triphosphate
keywords: ATP depletion assay; Chemotype sensitivity; Cytotoxicity assay; Exploratory Toxicology Studies; HepG2; THLE; TPSA; cLogP