title: FOXL2 suppresses proliferation, invasion and promotes apoptosis of cervical cancer cells.
abstract: FOXL2 is a transcription factor that is essential for ovarian function and maintenance, the germline mutations of which give rise to the blepharophimosis ptosis epicanthus inversus syndrome (BPES), often associated with premature ovarian failure. Recently, its mutations have been found in ovarian granulosa cell tumors (OGCTs). In this study, we measured the expression of FOXL2 in cervical cancer by immunohistochemistry and its mRNA level in cervical cancer cell lines Hela and Siha by RT-PCR. Then we overexpressed FOXL2 in Hela cells and silenced it in Siha cells by plasmid transfection and verified using western blotting. When FOXL2 was overexpressed or silenced, cells proliferation and apoptosis were determined by Brdu assay and Annexin V/PI detection kit, respectively. In addition, we investigated the effects of FOXL2 on the adhesion and invasion of Hela and Siha cells. Finally, we analyzed the influences of FOXL2 on Ki67, PCNA and FasL by flow cytometry. The results showed that FOXL2 was highly expressed in cervical squamous cancer. Overexpressing FOXL2 suppressed Hela proliferation and facilitated its apoptosis. Silencing FOXL2 enhanced Siha proliferation and inhibited its apoptosis. Meanwhile, silencing FOXL2 promoted Siha invasion, but it had no effect on cells adhesion. In addition, overexpressing FOXL2 decreased the expression of Ki67 in Hela and Siha cells. Therefore, our results suggested that FOXL2 restrained cells proliferation and enhanced cells apoptosis mainly through decreasing Ki67 expression.
authors.affiliation: Plastic Surgery Institute of Weifang Medical University Weifang, 261041, Shandong Province, China ; Department of Traumatic Orthopedics, The 89th Hospital of PLA Weifang, 261041, Shandong Province, China.
authors.forename: Xing-Long
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Peking University Third Hospital Beijing, 100191, China.
authors.forename: Yu-Han
authors.initials: YH
authors.lastname: Meng
authors.affiliation: Department of Traumatic Orthopedics, The 89th Hospital of PLA Weifang, 261041, Shandong Province, China.
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Wang
authors.affiliation: Plastic Surgery Institute of Weifang Medical University Weifang, 261041, Shandong Province, China.
authors.forename: Biao-Bing
authors.initials: BB
authors.lastname: Yang
authors.affiliation: Plastic Surgery Institute of Weifang Medical University Weifang, 261041, Shandong Province, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Plastic Surgery Institute of Weifang Medical University Weifang, 261041, Shandong Province, China.
authors.forename: Sheng-Jian
authors.initials: SJ
authors.lastname: Tang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D000328:Adult; D017209:Apoptosis / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D053222:Fas Ligand Protein / Q000235:genetics / Q000502:physiology; D005260:Female; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics / Q000502:physiology; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000502:physiology; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*
chemical_list: C421604:FOXL2 protein, human; D053222:Fas Ligand Protein; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen
keywords: FOXL2; apoptosis; cervical cancer; invasiveness; proliferation