title: Visualization of hormone binding proteins in vivo based on Mn-doped CdTe QDs.
abstract: Daminozide (B9) is a growth inhibitor with important regulatory roles in plant growth and development. Locating and quantifying B9-binding proteins in plant tissues will assist in investigating the mechanism behind the signal transduction of B9. In this study, red fluorescent Mn-doped CdTe quantum dots (CdTeMn QDs) were synthesized by a high-temperature hydrothermal process. Since CdTeMn QDs possess a maximum fluorescence emission peak at 610nm, their fluorescence properties are more stable than those of CdTe QDs. A B9-CdTeMn probe was synthesized by coupling B9 with CdTeMn QDs. The fluorescence intensity of the probe is double that of CdTeMn QDs; its fluorescence stability is also superior under different ambient conditions. The probe retains the biological activity of B9 and is unaffected by interference from the green fluorescent protein present in plants. Therefore, we used this probe to label B9-binding proteins selectively in root tissue sections of mung bean seedlings. These proteins were observed predominantly on the surfaces of the cell membranes of the cortex and epidermal parenchyma.
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors.forename: Fang fei
authors.initials: Ff
authors.lastname: Liu
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China. Electronic address: yuyhs@scnu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors.forename: Bi xia
authors.initials: Bx
authors.lastname: Lin
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors.forename: Xiao gang
authors.initials: Xg
authors.lastname: Hu
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors.forename: Yu juan
authors.initials: Yj
authors.lastname: Cao
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors.forename: Jian zhong
authors.initials: Jz
authors.lastname: Wu
mesh_terms: D019187:Cadmium Compounds / Q000032:analysis / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D008345:Manganese / Q000032:analysis / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D045663:Quantum Dots / Q000032:analysis* / Q000378:metabolism; D036226:Seedlings / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence; D013386:Succinates / Q000378:metabolism*; D013691:Tellurium / Q000032:analysis / Q000378:metabolism
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013386:Succinates; D008345:Manganese; C014499:daminozide; D013691:Tellurium; C028337:cadmium telluride
keywords: Binding proteins; Daminozide; Labeling; Quantum dots