title: Peptide nucleic acid-mediated aggregation of reduced graphene oxides and label-free detection of DNA mutation.
abstract: We have investigated peptide nucleic acid (PNA)-mediated aggregation of reduced graphene oxide (rGO) sheets. Addition of PNA into suspension of rGOs resulted in aggregation of rGOs, which could be easily detected with the naked eye. To elucidate the mechanism of rGO aggregation, we monitored the reaction by transmission electron microscopy, zeta potential measurement, and UV-vis spectroscopy. Our findings suggest that PNA adsorbed on the rGO surface and then acted as a cross-linker to induce aggregation. We also tested the effects of different nucleic acids on rGO aggregation and found that not only the single-stranded DNA, but also the PNA-DNA complex, could stabilize the suspension against aggregation through electrostatic repulsion. Based on our understanding on rGO aggregation, we attempted to detect mutations in epidermal growth factor receptor (EGFR) by designing PNA probe to be complementary to the mutant type DNA sequence. Our results showed that PNA-mediated rGO aggregation could successfully be used to detect mutations in EGFR with high selectivity and sensitivity.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University , Seoul, Republic of Korea.
authors.forename: Taegyeong
authors.initials: T
authors.lastname: Kang
authors.forename: Hoon
authors.initials: H
authors.lastname: Choi
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Joo
authors.forename: So Yeong
authors.initials: SY
authors.lastname: Lee
authors.forename: Kyong-Ah
authors.initials: KA
authors.lastname: Yoon
authors.forename: Kangtaek
authors.initials: K
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D066246:ErbB Receptors / Q000235:genetics; D006108:Graphite / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009154:Mutation; D010087:Oxides / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis; D055672:Static Electricity
chemical_list: D010087:Oxides; D020135:Peptide Nucleic Acids; D006108:Graphite; D004247:DNA; D066246:ErbB Receptors