title: The expression of epithelial intercellular junctional proteins in the sinonasal tissue of subjects with chronic rhinosinusitis: a histopathologic study.
abstract: OBJECTIVE
  To evaluate the expression of five epithelial intercellular junctional proteins in the sinonasal tissue of subjects with chronic rhinosinusitis (CRS).
  
  
  METHODS
  Forty-one samples of nasal polyp tissue of CRS patients with nasal polyps (wNP), 20 ethmoid sinus mucosa of CRS patients without nasal polyps (sNP) and 19 nasal mucosa of controls were collected and assessed for the expression of zonulae occludens (ZO-1), claudin-1, E-cadherin and desmoglein-1 and -2 (DSG1, DSG2) using immunohistochemical staining. Interleukin (IL)-5, IL-6 and IL-8 concentrations in the tissues were also measured using ELISA.
  
  
  RESULTS
  The expression of ZO-1, claudin-1, DSG1 and DSG2 in the CRSwNP patient group and the expression of claudin-1, DSG1 and DSG2 of the CRSsNP patient group was significantly lower compared to that of the control group. Furthermore, the expression of DSG1 in the CRSwNP patient group was also significantly lower than in the CRSsNP patient group. In contrast, the expression of E-cadherin in the CRSwNP and the CRSsNP patient groups was significantly greater compared to the controls. The assessment of associations between the expression of the intercellular junctional proteins and cytokines demonstrated negative correlations between IL-5 and claudin-1, IL-6 and claudin-1, IL-6 and DSG2, IL-8 and DSG1, and IL-8 and DSG2. In contrast, a positive correlation was found between IL-8 and E-cadherin.
  
  
  CONCLUSIONS
  Differences in the expression of epithelial intercellular junctional proteins may play an important role in the pathogenesis of CRS.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Wang
authors.forename: Ruihan
authors.initials: R
authors.lastname: Wang
authors.forename: Mingyu
authors.initials: M
authors.lastname: Bo
authors.forename: Erzhong
authors.initials: E
authors.lastname: Fan
authors.forename: Su
authors.initials: S
authors.lastname: Duan
authors.forename: Luo
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015820:Cadherins / Q000378:metabolism*; D002908:Chronic Disease; D062445:Claudin-1 / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D051182:Desmogleins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012220:Rhinitis / Q000378:metabolism* / Q000473:pathology*; D012852:Sinusitis / Q000378:metabolism* / Q000473:pathology*; D055815:Young Adult; D062826:Zonula Occludens-1 Protein / Q000378:metabolism*
chemical_list: D015820:Cadherins; D062445:Claudin-1; D016207:Cytokines; D051182:Desmogleins; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein