title: Individually addressable multi-chamber electroporation platform with dielectrophoresis and alternating-current-electro-osmosis assisted cell positioning.
abstract: A multi-functional microfluidic platform was fabricated to demonstrate the feasibility of on-chip electroporation integrated with dielectrophoresis (DEP) and alternating-current-electro-osmosis (ACEO) assisted cell/particle manipulation. A spatial gradient of electroporation parameters was generated within a microchamber array and validated using normal human dermal fibroblast (NHDF) cells and red fluorescent protein-expressing human umbilical vein endothelial cells (RFP-HUVECs) with various fluorescent indicators. The edge of the bottom electrode, coinciding with the microchamber entrance, may act as an on-demand gate, functioning under either positive or negative DEP. In addition, at sufficiently low activation frequencies, ACEO vortices can complement the DEP to contribute to a rapid trapping/alignment of particles. As such, results clearly indicate that the microfluidic platform has the potential to achieve high-throughput screening for electroporation with spatial control and uniformity, assisted by DEP and ACEO manipulation/trapping of particles/cells into individual microchambers.
authors.affiliation: Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion-Israel Institute of Technology, Technion City 32000, Israel.
authors.forename: Sinwook
authors.initials: S
authors.lastname: Park
authors.affiliation: Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion-Israel Institute of Technology, Technion City 32000, Israel.
authors.forename: Dana Ben
authors.initials: DB
authors.lastname: Bassat
authors.affiliation: Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion-Israel Institute of Technology, Technion City 32000, Israel.
authors.forename: Gilad
authors.initials: G
authors.lastname: Yossifon