title: Determination of kaempferol and quercetin in Xindakang tablet by β-cyclodextrin modified micellar electrokinetic capillary chromatography.
abstract: A method was proposed to determine kaempferol and quercetin in Hippophae rhamnoides L medicinal preparation xindakang tablet by β-cyclodextrin modified micellar electrokinetic capillary chromatography. Under the optimized conditions: buffer solution of 20 mmol/L Na(2)B(4)O(7)-KH(2)PO(4) (pH 9.0)-20mmol/L SDS-6mmol/L β-CD-5%(v/v) MeCN, applied voltage of 16 kV and injection time of 8s, the two analytes were separated well within 10 minutes. The calibration was linear in the 0.02-0.80 and 0.02-0.70 mg/mL range for kaempferol and quercetin, respectively. The reproducibility based on migration time and peak height were 0.47% and1.87% for kaempferol, 0.55% and 2.02% for quercetin. The detection limits based on three times noise were 0.010 mg/mL and 0.008 mg/mL for kaempferol and quercetin, respectively. The developed method was utilized to analyze real samples and running recovery experiments with satisfactory results.
authors.affiliation: Analysis and Testing Centre, Dezhou University, University West Road 566, Dezhou, PR China / Institute of Materia Medica, Shandong Academy of Medical Sciences, Jingshi Road 18877, Jinan, PR China.
authors.forename: Yuanqi
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Analysis and Testing Centre, Dezhou University, University West Road 566, Dezhou, PR China.
authors.forename: Dunqing
authors.initials: D
authors.lastname: Wang
authors.affiliation: Analysis and Testing Centre, Dezhou University, University West Road 566, Dezhou, PR China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ning
authors.affiliation: Institute of Materia Medica, Shandong Academy of Medical Sciences, Jingshi Road 18877, Jinan, PR China.
authors.forename: Qingqiang
authors.initials: Q
authors.lastname: Yao
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D044949:Kaempferols / Q000032:analysis*; D008516:Medicine, Chinese Traditional; D011794:Quercetin / Q000032:analysis*; D013607:Tablets; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D044949:Kaempferols; D013607:Tablets; D047392:beta-Cyclodextrins; C006552:kaempferol; D011794:Quercetin; C031215:betadex