title: HIP-55/DBNL-dependent regulation of adrenergic receptor mediates the ERK1/2 proliferative pathway.
abstract: The activation of β-adrenergic receptors (β-ARs) plays a key role in regulating cardiac function. However, the detailed regulatory mechanisms of β-AR-induced fibrosis are still unclear. We used a proteomics approach to analyze the changes in protein expression patterns in cardiac fibrosis with β-AR stimulation. HIP-55 (also called debrin-like; DBNL) was revealed as a novel regulator in the signaling regulatory network with β-AR activation. Further studies of both HIP-55-overexpressed and -deficient cardiac fibroblasts indicated that HIP-55 negatively regulated β-AR-activated cardiac fibroblast proliferation and the proliferative signaling pathway may be associated with the extracellular signal-regulated protein kinase (ERK) activation. Our data provide a new mechanistic insight into the role of HIP-55 in β-AR-induced cardiac fibroblast proliferation and suggest a new treatment strategy for proliferative disorders.
authors.affiliation: Central Laboratory, Jilin University Second Hospital, Changchun 130041, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Yang
authors.forename: Aiju
authors.initials: A
authors.lastname: Tian
authors.forename: Zhizhen
authors.initials: Z
authors.lastname: Lv
authors.forename: Ningning
authors.initials: N
authors.lastname: Sun
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Youyi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zijian
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D005355:Fibrosis / Q000139:chemically induced; D005786:Gene Expression Regulation / Q000187:drug effects; D006335:Heart Injuries / Q000139:chemically induced / Q000473:pathology*; D007545:Isoproterenol / Q000009:adverse effects; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D018909:src Homology Domains / Q000235:genetics
chemical_list: C485513:Dbnl protein, rat; D008840:Microfilament Proteins; D011943:Receptors, Adrenergic, beta; D007545:Isoproterenol