title: Monitoring lectin interactions with carbohydrates.
abstract: Protein-carbohydrate interactions are often involved in the first step of infection and Pseudomonas aeruginosa produces several proteins that are able to bind specifically to glycan epitopes present on host epithelia. The experimental approaches for studying protein-carbohydrate interaction have been inspired, with some adaptations, from those commonly used for protein-protein or protein-ligand interactions. A range of methods are described herein for detecting lectin activity, screening for monosaccharide or oligosaccharide specificity, determining the affinity of binding together with thermodynamics and kinetics parameters, and producing crystal of lectin-carbohydrate complexes for further structural studies.
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), UMR7255 CNRS-Aix Marseille Université, Institut de Microbiologie de la Méditerranée, 31 Chemin Joseph Aiguier, Marseille Cédex 20, 13402, France, bentzman@imm.cnrs.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: de Bentzmann
authors.forename: Annabelle
authors.initials: A
authors.lastname: Varrot
authors.forename: Anne
authors.initials: A
authors.lastname: Imberty
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002151:Calorimetry; D002241:Carbohydrates / Q000737:chemistry*; D018360:Crystallography, X-Ray; D006386:Hemagglutination Tests; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D011817:Rabbits; D020349:Surface Plasmon Resonance
chemical_list: D002241:Carbohydrates; D037102:Lectins; D008024:Ligands