title: Optimization of extraction and enrichment of steroidal alkaloids from bulbs of cultivated Fritillaria cirrhosa.
abstract: The bulbs of cultivated Fritillaria cirrhosa (BCFC) are used in China both for food and folk medicine due to its powerful biological activities. The aim of this study is to optimize the extraction and enrichment conditions of alkaloids from BCFC. Firstly, the orthogonal experimental design was used to optimize and evaluate four variables (ethanol concentration, solid-liquid ratio, extraction time, and temperature). Thereafter, resin adsorption was as a means to enrich alkaloids. Among 16 tested resins, H-103 resin presented higher adsorption capacity and desorption ratio. The equilibrium experimental data of the adsorption of total alkaloids, imperialine, and peimisine were well-fitted to the pseudo-first-order kinetics model, Langmuir and Freundlich isotherms models. Finally, in order to optimize the parameters for purifying alkaloids, dynamic adsorption and desorption tests were carried out. After one run treatment with H-103 resin, the contents of total alkaloids, imperialine, and peimisine in the product were 21.40-, 18.31-, and 22.88-fold increased with recovery yields of 94.43%, 90.57%, and 96.16%, respectively.
authors.affiliation: Department of Pharmacognosy, West China College of Pharmacy, Sichuan University, No. 17, Duan 3, Renmin Nan Road, Chengdu 610041, China.
authors.forename: Dongdong
authors.identifier: 0000-0002-6195-4428
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Pharmacognosy, West China College of Pharmacy, Sichuan University, No. 17, Duan 3, Renmin Nan Road, Chengdu 610041, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Pharmacognosy, West China College of Pharmacy, Sichuan University, No. 17, Duan 3, Renmin Nan Road, Chengdu 610041, China.
authors.forename: Qingdan
authors.initials: Q
authors.lastname: Du
authors.affiliation: Department of Pharmacognosy, West China College of Pharmacy, Sichuan University, No. 17, Duan 3, Renmin Nan Road, Chengdu 610041, China.
authors.forename: Nanyi
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Pharmacognosy, West China College of Pharmacy, Sichuan University, No. 17, Duan 3, Renmin Nan Road, Chengdu 610041, China.
authors.forename: Simei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Pharmacognosy, West China College of Pharmacy, Sichuan University, No. 17, Duan 3, Renmin Nan Road, Chengdu 610041, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmacognosy, West China College of Pharmacy, Sichuan University, No. 17, Duan 3, Renmin Nan Road, Chengdu 610041, China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification*; D031410:Fritillaria / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000470:Alkaloids; D013256:Steroids