title: Relationship between toll-like receptor 2 Arg677Trp and Arg753Gln and toll-like receptor 4 Asp299Gly polymorphisms and cytomegalovirus infection.
abstract: OBJECTIVE
  The association among specific single-nucleotide polymorphisms (SNPs) in TLR2 (Arg677Trp, Arg753Gln) and TLR4 (Asp299Gln) and human cytomegalovirus (CMV) infection was studied in infants and adults.
  
  
  METHODS
  The TLR2 and TLR4 polymorphisms were genotyped in 151 patients with CMV infections and in 78 unrelated healthy individuals. Genotyping was performed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments. The viral load was measured by quantitative real-time PCR.
  
  
  RESULTS
  Almost all of the patients with CMV infections were wild-type homozygotes without TLR2 and TLR4 polymorphisms. No significant differences in TLR2 and TLR4 polymorphisms were observed between infants with or without CMV infection. Compared with adults with CMV infections, heterozygosity for the TLR2 Arg677Trp and TLR4 Asp299Gly SNPs was detected more frequently in healthy individuals (p<0.05). Logistic regression analysis showed that the wild-type TLR2 genotype was associated with an increased risk of CMV infection and that heterozygosity for TLR2 and TLR4 SNPs diminished the risk of CMV infection in adult patients. An association between CMV load and the TLR4 SNP was found.
  
  
  CONCLUSIONS
  Our results suggest that the wild-type TLR2 genotype may be a risk factor for CMV replication in adult patients.
authors.affiliation: Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St., 93-232 Lodz, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Jabłońska
authors.affiliation: Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St., 93-232 Lodz, Poland. Electronic address: eparadowska@cbm.pan.pl.
authors.forename: Edyta
authors.initials: E
authors.lastname: Paradowska
authors.affiliation: Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St., 93-232 Lodz, Poland.
authors.forename: Mirosława
authors.initials: M
authors.lastname: Studzińska
authors.affiliation: Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St., 93-232 Lodz, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Suski
authors.affiliation: Department of Foetal-Maternal Medicine and Gynaecology, Third Chair of Gynaecology and Obstetrics, Medical University, Lodz, Poland; Department of Foetal-Maternal Medicine and Gynaecology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Nowakowska
authors.affiliation: Third Department of Paediatrics, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wiśniewska-Ligier
authors.affiliation: Third Department of Paediatrics, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Woźniakowska-Gęsicka
authors.affiliation: Department of Foetal-Maternal Medicine and Gynaecology, Third Chair of Gynaecology and Obstetrics, Medical University, Lodz, Poland; Department of Foetal-Maternal Medicine and Gynaecology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Wilczyński
authors.affiliation: Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St., 93-232 Lodz, Poland.
authors.forename: Zbigniew J
authors.initials: ZJ
authors.lastname: Leśnikowski
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D019943:Amino Acid Substitution; D003062:Codon; D003587:Cytomegalovirus; D003586:Cytomegalovirus Infections / Q000235:genetics* / Q000821:virology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D019562:Viral Load; D055815:Young Adult
chemical_list: D003062:Codon; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4
keywords: Cytomegalovirus; Single-nucleotide polymorphism; Toll-like receptors