title: Oxidatively modified proteins in the serous subtype of ovarian carcinoma.
abstract: Serous subtype of ovarian cancer is considered to originate from fallopian epithelium mucosa that has been exposed to physiological changes resulting from ovulation. Ovulation influences an increased in inflammation of epithelial ovarian cells as results of constant exposure of cells to ROS. The imbalance between ROS and antioxidant capacities, as well as a disruption of redox signaling, causes a wide range of damage to DNA, proteins, and lipids. This study applied spectrophotometric, dinitrophenylhydrazone (DNPH) assay, two-dimensional gel electrophoresis, and Western blot analyses to assess the levels of oxidatively modified proteins in 100 primary serous epithelial ovarian carcinoma and normal/surrounding tissues. These samples were obtained from 56 Caucasian and 44 African-American patients within the age range of 61 ± 10 years. Analyses showed that the levels of reactive protein carbonyl groups increased as stages progressed to malignancy. Additionally, the levels of protein carbonyls in serous ovarian carcinoma among African Americans are 40% (P < 0.05) higher relative to Caucasian at similar advanced stages. Results suggest that oxidative stress is involved in the modification of carbonyl protein groups, leading to increased aggressiveness of epithelial ovarian tumors and may contribute to the disease's invasiveness among African Americans.
authors.affiliation: Department of Medicine, Morehouse School of Medicine, Atlanta, GA 30310, USA.
authors.forename: Sharifeh
authors.initials: S
authors.lastname: Mehrabi
authors.affiliation: Comprehensive Cancer Center University of Alabama at Birmingham, Birmingham, AL 35249, USA.
authors.forename: Edward E
authors.initials: EE
authors.lastname: Partridge
authors.affiliation: Department of Physiology, Morehouse School of Medicine, Atlanta, GA 30310, USA.
authors.forename: William
authors.identifier: 0000-0002-5657-1852
authors.initials: W
authors.lastname: Seffens
authors.affiliation: Department of Physiology, Morehouse School of Medicine, Atlanta, GA 30310, USA.
authors.forename: Xuebiao
authors.initials: X
authors.lastname: Yao
authors.affiliation: Department of Medicine, Morehouse School of Medicine, Atlanta, GA 30310, USA.
authors.forename: Felix O
authors.initials: FO
authors.lastname: Aikhionbare
mesh_terms: D000368:Aged; D018284:Cystadenocarcinoma, Serous / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000378:metabolism; D018384:Oxidative Stress; D050050:Protein Carbonylation; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*
chemical_list: D011506:Proteins