title: Inverting microwell array chip for the cultivation of human induced pluripotent stem cells with controlled aggregate size and geometrical arrangement.
abstract: We present a novel cell culture chip, namely, "inverting microwell array chip," for cultivation of human induced pluripotent stem cells. The chip comprises a lower hydrogel microwell array and an upper polystyrene culture surface. We demonstrate the formation of uniform cellular aggregates in the microwell array, and after inversion, a culture with controlled aggregate size and geometrical arrangement on the polystyrene surface. Here, we report effects of cell concentrations on a cultivation sequence in the chip.
authors.affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 5, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8568, Japan.
authors.forename: Taku
authors.initials: T
authors.lastname: Satoh
authors.affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 5, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8568, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Sugiura
authors.affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 5, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8568, Japan.
authors.forename: Kimio
authors.initials: K
authors.lastname: Sumaru
authors.affiliation: Tokyo Electron Limited, 5-3-1 Akasaka, Minato-ku, Tokyo 107-6325, Japan.
authors.forename: Shigenori
authors.initials: S
authors.lastname: Ozaki
authors.affiliation: Tokyo Electron Limited, 5-3-1 Akasaka, Minato-ku, Tokyo 107-6325, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Gomi
authors.affiliation: Tokyo Electron Limited, 5-3-1 Akasaka, Minato-ku, Tokyo 107-6325, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Kurakazu
authors.affiliation: Tokyo Electron Limited, 5-3-1 Akasaka, Minato-ku, Tokyo 107-6325, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Oshima
authors.affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 5, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8568, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kanamori