title: Dystroglycan depletion inhibits the functions of differentiated HL-60 cells.
abstract: Dystroglycan has recently been characterized in blood tissue cells, as part of the dystrophin glycoprotein complex but to date nothing is known of its role in the differentiation process of neutrophils. We have investigated the role of dystroglycan in the human promyelocytic leukemic cell line HL-60 differentiated to neutrophils. Depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated HL-60 cells, including chemotaxis, respiratory burst, phagocytic activities and expression of markers of differentiation. These findings strongly implicate dystroglycan as a key membrane adhesion protein involved in the differentiation process in HL-60 cells.
authors.affiliation: Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico.
authors.forename: Alma Delia
authors.initials: AD
authors.lastname: Martínez-Zárate
authors.affiliation: Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico.
authors.forename: Ivette
authors.initials: I
authors.lastname: Martínez-Vieyra
authors.affiliation: Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico.
authors.forename: Lea
authors.initials: L
authors.lastname: Alonso-Rangel
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav-IPN), Mexico City, Mexico.
authors.forename: Bulmaro
authors.initials: B
authors.lastname: Cisneros
authors.affiliation: Department of Biomedical Science, University of Sheffield, Sheffield, UK.
authors.forename: Steve J
authors.initials: SJ
authors.lastname: Winder
authors.affiliation: Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico. Electronic address: dcereced@prodigy.net.mx.
authors.forename: Doris
authors.initials: D
authors.lastname: Cerecedo
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D049030:Dystroglycans / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology*; D010587:Phagocytosis; D010641:Phenotype; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D016897:Respiratory Burst
chemical_list: D015415:Biomarkers; C483081:DAG1 protein, human; D034741:RNA, Small Interfering; D049030:Dystroglycans
keywords: Differentiation; Dystroglycan; HL-60 cells; Migration; Phagocytosis; Respiratory burst activity