title: Breed-specific hematologic reference intervals in healthy adult Dogues de Bordeaux.
abstract: BACKGROUND
  There is an increasing interest for breed-specific reference intervals in veterinary medicine. In a previous study, breed-specific biochemical reference intervals (RIs) have been established for Dogues de Bordeaux (DDBs). This breed is predisposed to familial juvenile glomerulonephropathy and hypothyroidism, and would benefit from hematologic RI.
  
  
  OBJECTIVE
  The purpose was de novo establishment of breed-specific hematologic RIs for the DDB in accordance with the International Federation of Clinical Chemistry and Clinical and Laboratory Standards Institute guidelines.
  
  
  METHODS
  One hundred and twenty DDBs from France and Belgium were recruited. CBCs were determined with the Sysmex XT-2000iV analyzer within 12 hours of blood collection. RIs were determined using the nonparametric method. Effects of sex, age, and face mask color were studied.
  
  
  RESULTS
  RIs were determined in 58 healthy dogs. DDBs had higher RIs for HGB, HCT, MCV, MCHC, and mean platelet volume, and lower RIs for reticulocytes counts, platelets by impedance (PLT-I) and optical count (PLT-O), and plateletcrit when compared with generic canine RIs. Age significantly affected RIs for HGB, HCT, MCHC, WBC, neutrophil, lymphocyte, and monocyte counts.
  
  
  CONCLUSIONS
  The generic canine RIs established in the same laboratory with analogous preanalytical and analytical variations did not differ significantly from breed-specific RIs, and thus have no significant impact on clinical decision making; however, breed-specific RIs are advised for some RBC and all platelet-related variables to avoid erroneous suspicion of polycythemia and thrombocytopenia when using general canine RIs for evaluation of DDB.
authors.affiliation: Internal Medicine Unit , Institut National Polytechnique, Ecole Nationale Vétérinaire de Toulouse (INP-ENVT), Toulouse, France.
authors.forename: R
authors.initials: R
authors.lastname: Lavoué
authors.forename: A
authors.initials: A
authors.lastname: Geffré
authors.forename: J P
authors.initials: JP
authors.lastname: Braun
authors.forename: D
authors.initials: D
authors.lastname: Peeters
authors.forename: F
authors.initials: F
authors.lastname: Granat
authors.forename: N
authors.initials: N
authors.lastname: Bourgès-Abella
authors.forename: C
authors.initials: C
authors.lastname: Trumel
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001947:Breeding; D004285:Dogs / Q000097:blood* / Q000145:classification; D005260:Female; D006403:Hematologic Tests / Q000592:standards / Q000662:veterinary*; D008297:Male; D011446:Prospective Studies; D012016:Reference Values; D013045:Species Specificity
chemical_list: D015415:Biomarkers
keywords: Brachicephalic canine; Sysmex; genetic polymorphism; mastiff