title: Nitro-oleic acid downregulates lipoprotein-associated phospholipase A2 expression via the p42/p44 MAPK and NFκB pathways.
abstract: Nitro-oleic acid (OA-NO2), acting as anti-inflammatory signaling mediators, are involved in multiple signaling pathways. Lipoprotein-associated phospholipase A2 (Lp-PLA2) is well known as a cardiovascular risk biomarker. Our results showed that OA-NO2 downregulated the expression of Lp-PLA2 in a time- and dose-dependent manner, whereas native OA had no such effect. Furthermore, OA-NO2 could repress Lp-PLA2 expression in the peripheral blood mononuclear cells of apo CIII-transgenic (apo CIII TG) pigs, which exhibited higher Lp-PLA2 expression and activity than did wild-type (WT) pigs. OA-NO2 inhibited Lp-PLA2 expression in macrophages, independent of nitric oxide formation and PPARγ-activation. However, OA-NO2 downregulates Lp-PLA2 by inhibiting the p42/p44 mitogen-activated protein kinase (MAPK) and the nuclear factor κB (NFκB) pathways. When used to mediate anti-inflammatory signaling, the regulation of inflammatory cytokines and SOD by OA-NO2 might be associated with the reduction of Lp-PLA2. These results suggested that OA-NO2 might exert a vascular-protective effect partially via Lp-PLA2 inhibition.
authors.affiliation: 1] Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University [2].
authors.forename: Gangqi
authors.initials: G
authors.lastname: Wang
authors.affiliation: 1] Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University [2].
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Han
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University.
authors.forename: Nannan
authors.initials: N
authors.lastname: Guo
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University.
authors.forename: Qi
authors.initials: Q
authors.lastname: Song
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University.
authors.forename: Longquan
authors.initials: L
authors.lastname: Quan
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University.
authors.forename: Tiedong
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jilin University.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Han
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University.
authors.forename: Daxin
authors.initials: D
authors.lastname: Pang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Tang
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000235:genetics*; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D015536:Down-Regulation; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D019301:Oleic Acid / Q000494:pharmacology*; D047495:PPAR gamma / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D013552:Swine
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D047495:PPAR gamma; D019301:Oleic Acid; D009569:Nitric Oxide; D013482:Superoxide Dismutase; D048052:Mitogen-Activated Protein Kinase 3; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase