title: Efficient chromatographic reduction of ovalbumin for egg-based influenza virus purification.
abstract: Vaccination is the most effective prevention strategy to avoid influenza infection and for protection of large populations. The vast majority of influenza vaccines are still produced with allantoic fluid from fertilized chicken eggs. The presence of ovalbumin, which can constitute over 60% of the total protein content in allantoic fluid, can result in severe allergies. Consequently, efficient reduction of ovalbumin is critical during egg based vaccine manufacturing. Here we present Capto Core 700, a novel core bead chromatographic flow through mode resin for removal of ovalbumin and compare it to sucrose zonal gradient ultracentrifugation, which is the industry standard for egg-based vaccine production. The results demonstrate that core bead chromatography is fully comparable to zonal centrifugation in removing ovalbumin to meet regulatory requirements. Furthermore, the scalability and the shorter process times of this method have the potential to significantly improve the productivity and economy for industrial production compared to zonal centrifugation.
authors.affiliation: BioProcessing R&D, GE Healthcare Bio-Sciences AB, Björkgatan 30, SE-751 84 Uppsala, Sweden. Electronic address: Hans.Blom@ge.com.
authors.forename: Hans
authors.initials: H
authors.lastname: Blom
authors.affiliation: BioProcessing R&D, GE Healthcare Bio-Sciences AB, Björkgatan 30, SE-751 84 Uppsala, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Åkerblom
authors.affiliation: Institute for Translational Vaccinology, Antonie van Leeuwenhoeklaan 9, Pb 450, 3720 AL Bilthoven, The Netherlands.
authors.forename: Theone
authors.initials: T
authors.lastname: Kon
authors.affiliation: Institute for Translational Vaccinology, Antonie van Leeuwenhoeklaan 9, Pb 450, 3720 AL Bilthoven, The Netherlands.
authors.forename: Sabah
authors.initials: S
authors.lastname: Shaker
authors.affiliation: Institute for Translational Vaccinology, Antonie van Leeuwenhoeklaan 9, Pb 450, 3720 AL Bilthoven, The Netherlands.
authors.forename: Leo
authors.initials: L
authors.lastname: van der Pol
authors.affiliation: BioProcessing R&D, GE Healthcare Bio-Sciences AB, Björkgatan 30, SE-751 84 Uppsala, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Lundgren
mesh_terms: D000818:Animals; D002645:Chickens; D002845:Chromatography; D053122:Influenza A Virus, H3N2 Subtype; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D010047:Ovalbumin / Q000737:chemistry*; D010063:Ovum / Q000821:virology*; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D010047:Ovalbumin
keywords: Allantoic fluid; Core bead chromatography; Influenza; Ovalbumin; Ultracentrifugation