title: Chromosomal integration of transcriptional fusions.
abstract: The characterization and parameterization of promoters is crucial for the study of gene regulatory networks. While a number of techniques are available for this purpose, the use of reporter fusions integrated in the chromosome of a bacterial host affords precise quantification of transcriptional responses with high reproducibility. Here, we describe the integration of green fluorescent protein (GFP) and lacZ reporter cassettes using either mini-Tn7-based vectors or homologous chromosomal recombination to analyze gene regulation at transcriptional and post-transcriptional levels.
authors.affiliation: Faculdade de Medicina, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Rafael
authors.initials: R
authors.lastname: Silva-Rocha
authors.forename: Víctor
authors.initials: V
authors.lastname: de Lorenzo
mesh_terms: D019677:Artificial Gene Fusion / Q000379:methods*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D059765:Homologous Recombination / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000235:genetics*; D011401:Promoter Regions, Genetic; D016958:Pseudomonas putida / Q000235:genetics*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins; D001616:beta-Galactosidase