title: The EF-hand calcium-binding protein tescalcin is a potential oncotarget in colorectal cancer.
abstract: Tescalcin (TESC) is an EF-hand calcium binding protein that is differentially expressed in several tissues, however it is not reported that the expression and functional roles of TESC in colorectal cancer. Levels of messenger RNA (mRNA) and protein expression of TESC in colorectal cancer tissues were assessed using RT-PCR, real time PCR, immunohistochemistry, and clinicopathologic analyses. Quantitative analysis of TESC levels in serum specimens was performed using sandwich ELISA. Colorectal cancer cells transfected with TESC small interfering RNA and short hairpin RNA were examined in cell proliferation assays, phospho-MAPK array, and mouse xenograft models. Here we demonstrated that TESC is overexpressed in colorectal cancer (CRC), but was not expressed in normal mucosa and premalignant dysplastic lesions. Furthermore, serum TESC levels were elevated in patients with CRC. Knockdown of TESC inhibited the Akt-dependent NF-κB pathway and decreased cell survival in vitro. Depletion of TESC reduced tumor growth in a CRC xenograft model. Thus, TESC is a potential diagnostic marker and oncotarget in colorectal cancer.
authors.affiliation: Medical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Kang
authors.forename: Seung Ro
authors.initials: SR
authors.lastname: Han
authors.forename: Jong-Tae
authors.initials: JT
authors.lastname: Kim
authors.forename: Seon-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Young Il
authors.initials: YI
authors.lastname: Yeom
authors.forename: Jeong-Ki
authors.initials: JK
authors.lastname: Min
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Lee
authors.forename: Jae Wha
authors.initials: JW
authors.lastname: Kim
authors.forename: Suk Ran
authors.initials: SR
authors.lastname: Yoon
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Yoon
authors.forename: Kwan Soo
authors.initials: KS
authors.lastname: Hong
authors.forename: Geum-Sook
authors.initials: GS
authors.lastname: Hwang
authors.forename: Hee Cheol
authors.initials: HC
authors.lastname: Kim
authors.forename: Young-Ha
authors.initials: YH
authors.lastname: Lee
authors.forename: Hee Gu
authors.initials: HG
authors.lastname: Lee
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D053208:Kaplan-Meier Estimate; D008297:Male; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; C486009:TESC protein, human