title: [Creation of transgenic sugar beet lines expressing insect pest resistance genes cry1C and cry2A].
abstract: Impact of insect pests makes a significant limitation of the sugar beet crop yield. Integration of cry-genes of Bacillus thuringiensis into plant genome is one of the promising strategies to ensure plant resistance. The aim of this work was to obtain sugar beet lines (based on the MM 1/2 line) transformed with cry2A and cry1Cgenes. We have optimized transformation protocol and direct plant let regeneration protocol from leaf explants using 1 mg/l benzylaminopurine as well as 0,25 mg/l benzylaminopurine and 0,1 mg/l indole-butyric acid. Consequently, transgenic sugar beet lines transformed with vector constructs pRD400-cry1C and pRD400-cry2A have been obtained. PCR analysis revealed integration of cry2A and cry1C into genome of transgenic lines and expression of these genes in leaf tissues was shown by reverse transcription PCR.
authors.forename: D I
authors.initials: DI
authors.lastname: Litvin
authors.forename: V V
authors.initials: VV
authors.lastname: Sivura
authors.forename: V V
authors.initials: VV
authors.lastname: Kurilo
authors.forename: V D
authors.initials: VD
authors.lastname: Oleneva
authors.forename: A I
authors.initials: AI
authors.lastname: Emets
authors.forename: Ia B
authors.initials: IaB
authors.lastname: Blium
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001593:Benzyl Compounds / Q000494:pharmacology; D027461:Beta vulgaris / Q000187:drug effects / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D004175:Diptera / Q000472:pathogenicity* / Q000502:physiology; D004731:Endotoxins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D018745:Genome, Plant; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism; D007211:Indoles / Q000494:pharmacology; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D030821:Plants, Genetically Modified; D011687:Purines / Q000494:pharmacology; D019076:Transgenes
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001593:Benzyl Compounds; D004731:Endotoxins; D006460:Hemolysin Proteins; D007211:Indoles; D011687:Purines; C030956:insecticidal crystal protein, Bacillus Thuringiensis; C014612:indolebutyric acid; C480551:benzylaminopurine