title: Effect of cyclic mechanical stimulation on the expression of osteogenesis genes in human intraoral mesenchymal stromal and progenitor cells.
abstract: We evaluated the effects of mechanical stimulation on the osteogenic differentiation of human intraoral mesenchymal stem and progenitor cells (MSPCs) using the Flexcell FX5K Tension System that mediated cyclic tensile stretch on the cells. MSPCs were isolated from human mandibular retromolar bones and characterized using flow cytometry. The positive expression of CD73, CD90, and CD105 and negativity for CD14, CD19, CD34, CD45, and HLA-DR confirmed the MSPC phenotype. Mean MSPC doubling time was 30.4 ± 2.1 hrs. The percentage of lactate dehydrogenase (LDH) release showed no significant difference between the mechanically stimulated groups and the unstimulated controls. Reverse transcription quantitative real-time PCR revealed that 10% continuous cyclic strain (0.5 Hz) for 7 and 14 days induced a significant increase in the mRNA expression of the osteogenesis-specific markers type-I collagen (Col1A1), osteonectin (SPARC), bone morphogenetic protein 2 (BMP2), osteopontin (SPP1), and osteocalcin (BGLAP) in osteogenic differentiated MSPCs. Furthermore, mechanically stimulated groups produced significantly higher amounts of calcium deposited into the cultures and alkaline phosphatase (ALP). These results will contribute to a better understanding of strain-induced bone remodelling and will form the basis for the correct choice of applied force in oral and maxillofacial surgery.
authors.affiliation: Department of Orthopaedic Surgery, Medical University of Graz, Auenbruggerplatz 5, 8036 Graz, Austria.
authors.forename: Birgit
authors.identifier: 0000-0002-3156-7902
authors.initials: B
authors.lastname: Lohberger
authors.affiliation: Department of Orthopaedic Surgery, Medical University of Graz, Auenbruggerplatz 5, 8036 Graz, Austria.
authors.forename: Heike
authors.initials: H
authors.lastname: Kaltenegger
authors.affiliation: Department of Orthopaedic Surgery, Medical University of Graz, Auenbruggerplatz 5, 8036 Graz, Austria.
authors.forename: Nicole
authors.initials: N
authors.lastname: Stuendl
authors.affiliation: Department of Oral Surgery and Radiology, School of Dental Medicine, Medical University of Graz, Auenbruggerplatz 5, 8036 Graz, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Payer
authors.affiliation: Center for Medical Research, Medical University of Graz, Auenbruggerplatz 5, 8036 Graz, Austria.
authors.forename: Beate
authors.initials: B
authors.lastname: Rinner
authors.affiliation: Department of Orthopaedic Surgery, Medical University of Graz, Auenbruggerplatz 5, 8036 Graz, Austria.
authors.forename: Andreas
authors.identifier: 0000-0002-2598-2325
authors.initials: A
authors.lastname: Leithner
mesh_terms: D000293:Adolescent; D000328:Adult; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008334:Mandible / Q000166:cytology / Q000378:metabolism; D008437:Maxilla / Q000166:cytology / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D010012:Osteogenesis; D013314:Stress, Mechanical
chemical_list: D000943:Antigens, Differentiation