title: A validated RP-HPLC method to investigate finasteride in human skin after in vitro topically applying vesicular nanocarrier.
abstract: The pharmacotherapeutic efficiency of topical drug delivery systems is mainly dominated by the skin distribution of therapeutic agents. In this work, a sensitive, rapid and fully-validated reversed-phase high performance liquid chromatography (RP-HPLC) method was developed to determine finasteride in human cadaver skin after different vesicular formulations were applied. Drug in different depth of skin layers were measured with an EclipseXDB-C18 column. The mobile phase consisted of 75% (v/v) methanol containing 0.2% phosphoric acid buffered to pH 3.0 with triethylamine under isocratic conditions. The system was operated at 40°C and the mobile phase flow rate was set at 1 mL/min. The standard-calibration curve was linear within range of 5 to 200 ng/ml with correlation coefficient 0.9996. The intra-assay precision was less than 3.9% while the inter-assay precision was less than 7.1% with the bias range of -8.6 to 4.1%. This method was found to be specific, accurate, and sensitive and was successfully used to determine the accumulation of finasteride after in-vitro percutaneous delivery by liposomal or ethosomal drug delivery nanocarriers.
authors.affiliation: Department of Pharmacy, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Feiyue
authors.initials: F
authors.lastname: Zheng
authors.affiliation: The First Affiliated Hospital, College of Medicine and College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Rao
authors.affiliation: The First Affiliated Hospital, College of Medicine and College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lou
authors.affiliation: The First Affiliated Hospital, College of Medicine and College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Lu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016503:Drug Delivery Systems; D018120:Finasteride / Q000008:administration & dosage / Q000032:analysis*; D006801:Humans; D053758:Nanoparticles; D012867:Skin / Q000737:chemistry*
chemical_list: D018120:Finasteride