title: Conjugated linoleic acids attenuate LPS-induced pro-inflammatory gene expression by inhibiting the NF-κB translocation through PPARγ in buffalo granulosa cells.
abstract: OBJECTIVE
  In granulosa cells, TLR4-mediated LPS-induced immune response interferes with ovarian granulosa cell function.
  
  
  METHODS
  LPS-induced pro-inflammatory gene expression was monitored by real-time PCR and NF-κB was determined by the immuno-blotting and immuno-staining in granulosa cells in vitro.
  
  
  RESULTS
  The LPS (1 μg/mL) increased pro-inflammatory gene expression which was reverted back by treatment with TLR4 signaling inhibitor. Cotreatment of CLA (10 μm) with LPS did not show any effect on LPS-induced pro-inflammatory gene expression but granulosa cells pre-treated with CLA for 24 hr, attenuated LPS-induced pro-inflammatory gene expression and nuclear NF-κB. GW9662, a PPARγ-inhibitor, further increased the expression of pro-inflammatory genes.
  
  
  CONCLUSIONS
  The present findings reiterated that pre-treatment with CLA can prevent LPS-induced granulosa cells dysfunction.
authors.affiliation: Molecular Endocrinology Laboratory, Animal Biochemistry Division, National Dairy Research Institute, Karnal, India.
authors.forename: Vengala Rao
authors.initials: VR
authors.lastname: Yenuganti
authors.forename: Ravinder
authors.initials: R
authors.lastname: Ravinder
authors.forename: Dheer
authors.initials: D
authors.lastname: Singh
mesh_terms: D000813:Anilides / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002020:Buffaloes; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008070:Lipopolysaccharides; D016328:NF-kappa B / Q000378:metabolism*; D047495:PPAR gamma / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000494:pharmacology; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors
chemical_list: C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D044243:Linoleic Acids, Conjugated; D008070:Lipopolysaccharides; D016328:NF-kappa B; D047495:PPAR gamma; D010713:Phosphatidylcholines; D051197:Toll-Like Receptor 4; C472349:oxidized-L-alpha-1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine
keywords: GW9662; TLR4; infertility