title: Identification and characterization of a differentially expressed protein (CAPZB) in skeletal muscle between Meishan and Large White pigs.
abstract: Actin capping protein beta (CAPZB) protein was identified with considerable differences in the longissimus dorsi muscle between Large White and Meishan pigs using proteomics approach. However, in pigs, the information on CAPZB is very limited. In this study, we cloned and characterized the porcine actin capping protein beta (CAPZB) gene. In addition, we present two novel porcine CAPZB splice variants CAPZB1 and CAPZB2. CAPZB1 was expressed in all twenty tissues. However, CAPZB2 was predominantly expressed in the skeletal muscle and heart. In addition, the two isoforms had different expression profiles during the skeletal muscle development and between breeds. Moreover, the SNP T394G was identified in the coding region of the CAPZB gene, which was significantly associated with the carcass traits including the LFW, CFW, SFT and LEA. Data presented in our study suggests that the CAPZB gene may be a candidate gene of meat production trait and provides useful information for further studies on its roles in porcine skeletal muscle.
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China. Electronic address: wanglinjie@sicau.edu.cn.
authors.forename: Linjie
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Genetics, College of Life Sciences, Xinyang Normal University, Xinyang, Henan, PR China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhong
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Tang
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Yuanzhu
authors.initials: Y
authors.lastname: Xiong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051347:CapZ Actin Capping Protein / Q000235:genetics*; D003001:Cloning, Molecular; D005260:Female; D020869:Gene Expression Profiling; D005787:Gene Frequency; D005838:Genotype; D008297:Male; D008460:Meat; D008969:Molecular Sequence Data; D024510:Muscle Development / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D020033:Protein Isoforms / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*
chemical_list: D051347:CapZ Actin Capping Protein; D020033:Protein Isoforms
keywords: Actin capping protein; Alternative splicing; Pig; SNP; Skeletal muscle