title: Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia.
abstract: Prodigiosin, a member of the prodiginines, is a tripyrrole red pigment synthesized by Serratia and some other microbes. A bifurcated biosynthesis pathway of prodigiosin has been proposed in Serratia in which MBC (4-methoxy-2,2'-bipyrrole-5-carbaldehyde) and MAP (2-methyl-3-N-amyl-pyrrole) are synthesized separately and then condensed by PigC to form prodigiosin. The first step for the synthesis of MBC is the activation of L-proline by PigI, but its catalytic mechanism has remained elusive. To elucidate its mechanism, recombinant PigI was purified and crystallized. Crystals obtained by the sitting-drop method belonged to space group P1 and diffracted to 2.0 Å resolution, with unit-cell parameters a = 51.2, b = 62.8, c = 91.3 Å, α = 105.1, β = 90.1, γ = 92.2°. Matthews coefficient analysis suggested two molecules in the asymmetric unit, with a VM of 2.6 Å(3) Da(-1) and a solvent content of 52.69%.
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Han
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Ran
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Xiangdi
authors.initials: X
authors.lastname: Lou
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: He
authors.affiliation: Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Dongqing
authors.initials: D
authors.lastname: Xu
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Weiwu
authors.initials: W
authors.lastname: Wang
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D011088:DNA Ligases / Q000096:biosynthesis* / Q000737:chemistry*; D015971:Gene Expression Regulation, Enzymologic; D011353:Prodigiosin / Q000096:biosynthesis* / Q000737:chemistry*; D012705:Serratia / Q000201:enzymology*; D015398:Signal Transduction / Q000502:physiology
chemical_list: C076545:Ampligase; D011088:DNA Ligases; D011353:Prodigiosin
keywords: PigI; Serratia