title: Role of glutathione S transferase polymorphism in COPD with special reference to peoples living in the vicinity of the open cast coal mine of Assam.
abstract: BACKGROUND
  COPD may develop due to variation in the functioning of antioxidants along with smoking and environmental factors in genetically susceptible individuals. Since there are different views about the antioxidants responsible for detoxifying xenobiotic compound in the human body whose functional variation may lead to obstructive disease, this associative study has been taken up between GST gene polymorphism and COPD in populations exposed to coal dusts.
  
  
  METHODS
  Genotypes of the 70 COPD patients and 85 non COPD patients were determined by PCR based methods followed by multiplex PCR of GSTT1 and GSTM1 genes taking albumin gene as a control. Suspended particulate analyses were determined through the Respirable Dust sampler along with the FTIR analysis of the dust samples from the glass microfiber filters.
  
  
  RESULTS
  Dust sampling analysis reveals higher level of respirable suspended particulate matter, non respirable particulate matter, SO2 and NO2 present in air of the study site. FTIR analysis also suggests a higher concentration of organic silicone and aliphatic C-F compounds present in air of the study site and when spirometry was done, low lung function was observed among most of the subjects. GSTM1 null type was significantly associated with low lung function in smoker groups and the presence of at least one active allele (either GSTM1/GSTT1) seemed to have a protective role in the development of COPD.
  
  
  CONCLUSIONS
  GSTM1 (null genotype) appeared to be a risk factor for lower lung function in smokers living in the vicinity of coal mines. Apart from polluted environment and genetic susceptibility, mixed coal dust exposure rich in organic silicone and aliphatic C-F compounds also appears to be a factor for the low lung function.
authors.affiliation: Biotechnology Division, Council of Scientific & Industrial Research (CSIR) - North-East Institute of Science and Technology, Jorhat, Assam, India.
authors.forename: Tapan
authors.initials: T
authors.lastname: Dey
authors.affiliation: Biotechnology Division, Council of Scientific & Industrial Research (CSIR) - North-East Institute of Science and Technology, Jorhat, Assam, India.
authors.forename: Kabita
authors.initials: K
authors.lastname: Gogoi
authors.affiliation: Biotechnology Division, Council of Scientific & Industrial Research (CSIR) - North-East Institute of Science and Technology, Jorhat, Assam, India.
authors.forename: Bala Gopalan
authors.initials: BG
authors.lastname: Unni
authors.affiliation: Biotechnology Division, Council of Scientific & Industrial Research (CSIR) - North-East Institute of Science and Technology, Jorhat, Assam, India.
authors.forename: Munmi
authors.initials: M
authors.lastname: Kalita
authors.affiliation: Biotechnology Division, Council of Scientific & Industrial Research (CSIR) - North-East Institute of Science and Technology, Jorhat, Assam, India.
authors.forename: Moonmee
authors.initials: M
authors.lastname: Bharadwaz
authors.affiliation: Biotechnology Department, University of Science and Technology Meghalaya (USTM), 9th Mile, Meghalaya, India.
authors.forename: Minakshi
authors.initials: M
authors.lastname: Bhattacharjee
authors.affiliation: Biotechnology Division, Council of Scientific & Industrial Research (CSIR) - North-East Institute of Science and Technology, Jorhat, Assam, India.
authors.forename: Pranab Kumar
authors.initials: PK
authors.lastname: Boruah
authors.affiliation: Biotechnology Division, Council of Scientific & Industrial Research (CSIR) - North-East Institute of Science and Technology, Jorhat, Assam, India.
authors.forename: Thaneswar
authors.initials: T
authors.lastname: Bora
authors.affiliation: Biotechnology Division, Council of Scientific & Industrial Research (CSIR) - North-East Institute of Science and Technology, Jorhat, Assam, India.
authors.forename: Dibyajyoti
authors.initials: D
authors.lastname: Ozah
authors.affiliation: Biotechnology Division, Council of Scientific & Industrial Research (CSIR) - North-East Institute of Science and Technology, Jorhat, Assam, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kalita
mesh_terms: D000328:Adult; D000393:Air Pollutants / Q000378:metabolism / Q000633:toxicity; D003031:Coal; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D006798:Housing; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D008906:Mining; D011110:Polymorphism, Genetic; D029424:Pulmonary Disease, Chronic Obstructive / Q000201:enzymology* / Q000209:etiology / Q000235:genetics*; D012907:Smoking
chemical_list: D000393:Air Pollutants; D003031:Coal; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1