title: Circulating adipocyte fatty acid binding protein is increased in chronic and acute renal dysfunction.
abstract: OBJECTIVE
  The adipokine adipocyte fatty acid binding protein (AFABP) is positively associated with the development of the metabolic syndrome, diabetes mellitus, and cardiovascular disease. We hypothesized that AFABP also increases with deteriorating renal function.
  
  
  RESULTS
  Serum AFABP levels were quantified by enzyme linked immunosorbent assay in 532 patients with chronic kidney disease (CKD) covering the whole spectrum of estimated glomerular filtration rate (eGFR) categories from G1 to G5 (study population 1). Furthermore, AFABP was measured in 32 patients before and within 30 h after elective unilateral nephrectomy, a model of acute kidney dysfunction (AKD) (study population 2). Moreover, circulating AFABP was investigated in rats undergoing bilateral nephrectomy (BNE) as compared to sham-operated animals. Median serum AFABP levels adjusted for age, gender, and body mass index significantly increased with increasing eGFR category (G1: 22.0 μg/l; G2: 34.6 μg/l; G3: 56.7 μg/l; G4: 95.2 μg/l; and G5: 173.9 μg/l). Furthermore, renal dysfunction remained positively associated with AFABP in multivariate analysis in this cohort. In patients undergoing unilateral nephrectomy, AFABP increased significantly after surgery (42.1 μg/l) as compared to pre-surgical values (29.3 μg/l). Furthermore, relative changes of post-to-pre-surgical AFABP levels were independently associated with relative changes of post-to-pre-surgical creatinine concentrations. After BNE in rats, AFABP increased significantly as compared to sham-operated animals.
  
  
  CONCLUSIONS
  We show that AFABP is significantly elevated in CKD and AKD patients. Furthermore, measures of renal function are associated with circulating AFABP. Moreover, animal experiments indicate that AFABP levels strongly depend on renal function.
authors.affiliation: University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany; Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany. Electronic address: thomas.ebert@medizin.uni-leipzig.de.
authors.forename: T
authors.initials: T
authors.lastname: Ebert
authors.affiliation: University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany; Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany.
authors.forename: L M
authors.initials: LM
authors.lastname: Hopf
authors.affiliation: University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany; Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Wurst
authors.affiliation: University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Bachmann
authors.affiliation: University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany; Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kralisch
authors.affiliation: University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany; Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Lössner
authors.affiliation: University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany; Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Platz
authors.affiliation: University of Leipzig, Institute of Laboratory Medicine, 04103 Leipzig, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Kratzsch
authors.affiliation: University of Leipzig, Department of Urology, 04103 Leipzig, Germany.
authors.forename: J U
authors.initials: JU
authors.lastname: Stolzenburg
authors.affiliation: University of Leipzig, Department of Urology, 04103 Leipzig, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Dietel
authors.affiliation: University of Greifswald, Department of Physiology, 17495 Greifswald, Karlsburg, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Grisk
authors.affiliation: Hospital St. Georg, Division of Nephrology and KfH Renal Unit, 04129 Leipzig, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Beige
authors.affiliation: Outpatient Nephrology Care Unit, 04107 and 04178 Leipzig, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Anders
authors.affiliation: Outpatient Nephrology Care Unit, 04107 and 04178 Leipzig, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Bast
authors.affiliation: Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Klöting
authors.affiliation: University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Blüher
authors.affiliation: University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Stumvoll
authors.affiliation: University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany; Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Fasshauer
mesh_terms: D058186:Acute Kidney Injury / Q000097:blood*; D017667:Adipocytes / Q000378:metabolism*; D054392:Adipokines / Q000097:blood; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015992:Body Mass Index; D003404:Creatinine / Q000097:blood; D003430:Cross-Sectional Studies; D050556:Fatty Acid-Binding Proteins / Q000097:blood*; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009392:Nephrectomy; D051381:Rats; D051436:Renal Insufficiency, Chronic / Q000097:blood*; D055815:Young Adult
chemical_list: D054392:Adipokines; C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D003404:Creatinine
keywords: AFABP; AKI; Adipokines; CKD; Elimination