title: Coordinated and distinct functions of velvet proteins in Fusarium verticillioides.
abstract: Velvet-domain-containing proteins are broadly distributed within the fungal kingdom. In the corn pathogen Fusarium verticillioides, previous studies showed that the velvet protein F. verticillioides VE1 (FvVE1) is critical for morphological development, colony hydrophobicity, toxin production, and pathogenicity. In this study, tandem affinity purification of FvVE1 revealed that FvVE1 can form a complex with the velvet proteins F. verticillioides VelB (FvVelB) and FvVelC. Phenotypic characterization of gene knockout mutants showed that, as in the case of FvVE1, FvVelB regulated conidial size, hyphal hydrophobicity, fumonisin production, and oxidant resistance, while FvVelC was dispensable for these biological processes. Comparative transcriptional analysis of eight genes involved in the ROS (reactive oxygen species) removal system revealed that both FvVE1 and FvVelB positively regulated the transcription of a catalase-encoding gene, F. verticillioides CAT2 (FvCAT2). Deletion of FvCAT2 resulted in reduced oxidant resistance, providing further explanation of the regulation of oxidant resistance by velvet proteins in the fungal kingdom.
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Lan
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Hanxing
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Wenzhao
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Biological Science, Northern Illinois University, DeKalb, Illinois, USA.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Calvo
authors.affiliation: Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Harris
authors.affiliation: National Institute of Biological Sciences, Beijing, China.
authors.forename: She
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China lisj@im.ac.cn.
authors.forename: Shaojie
authors.initials: S
authors.lastname: Li
mesh_terms: D002374:Catalase / Q000235:genetics / Q000378:metabolism; D037341:Fumonisins / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D005670:Fusarium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D016877:Oxidants / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013172:Spores, Fungal / Q000254:growth & development
chemical_list: D037341:Fumonisins; D005656:Fungal Proteins; D016877:Oxidants; D017382:Reactive Oxygen Species; D002374:Catalase