title: [Cloning and expression of the mucin-related protein1 (Aamucin1) from salivary gland of Aedes albopictus].
abstract: OBJECTIVE
  To clone the mucin-related protein (Aamucin1) gene from salivary gland of Aedes albopictus Guangzhou isolate, and analyze the expression difference due to blood-feeding.
  
  
  METHODS
  Total RNA was extracted from the salivary gland. The coding region of Aamucin1 was amplified with a pair of specific primers by RT-PCR. The product was sequenced and analyzed by bioinformatics. Expression analysis was conducted by real-time RT-PCR.
  
  
  RESULTS
  The product of RT-PCR was 849 bp with encoding 283 amino acids. To compare with that from Ae. albopictus Rome strain, 13 amino acids were deleted at the C end, and Aamucin1 in Guangzhou isolate shared 58% identity in amino acids with that of Rome isolate. In addition, an alternative splicing was found in Aamucin1 and located in a proline enrich area by Protscan. To compare with that of non-blood-feeding (group SG), Aamucin1 was significantly down-regulated with 0.39 fold expression at zero time after engorged (group BSG_0, mosquitoes with abdominal distention from the first 2 hours after blood-feeding, P < 0.01) and 0.61 fold expression at the 24th hour after engorged (group BSG_24,mosquitoes from the 24th hours after blood-feeding, P > 005).
  
  
  CONCLUSIONS
  The full length of Aamucin1 gene of Ae. albopictus is cloned and it can be modulated by blood-feeding.
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Jin-Zhi
authors.initials: JZ
authors.lastname: Cheng
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.forename: Ru-Fang
authors.initials: RF
authors.lastname: Zhu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Jia-Hong
authors.initials: JH
authors.lastname: Wu
mesh_terms: D000330:Aedes / Q000235:genetics*; D000818:Animals; D003001:Cloning, Molecular; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D009077:Mucins / Q000235:genetics / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D009077:Mucins