title: Serum phenylalanine in preterm newborns fed different diets of human milk.
abstract: OBJECTIVE
  To evaluate phenylalanine plasma profile in preterm newborns fed different human milk diets.
  
  
  METHODS
  Twenty-four very-low weight preterm newborns were distributed randomly in three groups with different feeding types: Group I: banked human milk plus 5% commercial fortifier with bovine protein, Group II: banked human milk plus evaporated fortifier derived from modified human milk, Group III: banked human milk plus lyophilized fortifier derived from modified human milk. The newborns received the group diet when full diet was attained at 15 ± 2 days. Plasma amino acid analysis was performedon the first and last day of feeding. Comparison among groups was performed by statistical tests: one way ANOVA with Tukey's post-test using SPSS software, version 20.0 (IBM Corp, NY, USA), considering a significance level of 5%.
  
  
  RESULTS
  Phenylalanine levels in the first and second analysis were, respectively, in Group I: 11.9 ± 1.22 and 29.72 ± 0.73; in Group II: 11.72 ± 1.04 and 13.44 ± 0.61; and in Group III: 11.3 ± 1.18 and 15.42 ± 0.83 μmol/L.
  
  
  CONCLUSIONS
  The observed results demonstrated that human milk with fortifiers derived from human milk acted as a good substratum for preterm infant feeding both in the evaporated or the lyophilized form, without significant increases in plasma phenylalanine levels in comparison to human milk with commercial fortifier.
authors.affiliation: Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
authors.forename: Débora M
authors.initials: DM
authors.lastname: Thomaz
authors.affiliation: Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
authors.forename: Paula O
authors.initials: PO
authors.lastname: Serafin
authors.affiliation: Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil; Case Western Reserve University - RBCH, Cleveland, United States.
authors.forename: Durval B
authors.initials: DB
authors.lastname: Palhares
authors.affiliation: Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil; Universidade Católica Dom Bosco (UCDB), Campo Grande, MS, Brazil.
authors.forename: Luciana V M
authors.initials: LV
authors.lastname: Tavares
authors.affiliation: Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil. Electronic address: thayanagrance@yahoo.com.
authors.forename: Thayana R S
authors.initials: TR
authors.lastname: Grance
mesh_terms: D000818:Animals; D002417:Cattle; D004032:Diet / Q000379:methods*; D005260:Female; D005527:Food, Fortified / Q000032:analysis; D005612:Freeze Drying; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood*; D019102:Infant, Very Low Birth Weight / Q000097:blood*; D008297:Male; D017784:Milk Banks; D008894:Milk Proteins / Q000032:analysis; D008895:Milk, Human; D010649:Phenylalanine / Q000097:blood*
chemical_list: D008894:Milk Proteins; D010649:Phenylalanine
keywords: Aditivo para leite humano; Banked human milk; Fenilalanina plasmática; Human milk; Human milk fortifier; Leite humano; Leite humano de banco; Preterm newborns; Recém-nascido pré-termo; Serum phenylalanine