title: [Construction and expression of recombinant baculovirus with Schistosoma japonicum SjPP gene].
abstract: OBJECTIVE
  To express the soluble recombinant Schistosoma japonicum SjPP proteins in TN5B1-4 cells.
  
  
  METHODS
  The total RNA was extracted from adult worms of Schistosoma japonicum. The whole coding sequence of SjPP gene was synthesized by RT-PCR and cloned into donor plasmid. The recombinant donor pFastBac-SjPP was transformed into E.coli DH10Bac forming Bacmid-SjPP which was transfected into insect cell with cational lipofectin. The fusion protein SjPP was analyzed with SDS-PAGE and Western blotting.
  
  
  RESULTS
  The infective recombinant baculovirus Bacmid-SjPP was obtained and SjPP protein was expressed in insect cells.
  
  
  CONCLUSIONS
  The recombinant protein SjPP has been expressed in insect TN5B1-4 cells with proper antigenicity.
authors.forename: Li-Xiao
authors.initials: LX
authors.lastname: Yao
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Tao
authors.forename: Guan-Zhen
authors.initials: GZ
authors.lastname: Yang
authors.forename: Xiang-Fu
authors.initials: XF
authors.lastname: Wu
authors.forename: You-Min
authors.initials: YM
authors.lastname: Cai
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Lin
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D015801:Helminth Proteins / Q000096:biosynthesis*; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics; D012549:Schistosoma japonicum / Q000235:genetics*; D018411:Spodoptera / Q000166:cytology; D014162:Transfection
chemical_list: D015801:Helminth Proteins; D011994:Recombinant Proteins