title: qPCR for quantification of transgene expression and determination of transgene copy number.
abstract: Quantitative real-time PCR (qPCR) is a mature technology that can be used to accurately quantify the number of copies of a target nucleic acid in a sample. Here, we describe a method for using this technology to determine the copy number of a transgene stably integrated into a plant's genome and to ascertain the level of transgene expression.
authors.affiliation: Chemistry and Molecular Biosciences, The School of Biological Sciences, University of Queensland, St Lucia Campus, Brisbane, QLD, 4072, Australia, s.fletcher@uq.edu.au.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Fletcher
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D018628:Gene Dosage; D015870:Gene Expression; D020869:Gene Expression Profiling; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards*; D012015:Reference Standards; D019076:Transgenes; D014908:Triticum / Q000235:genetics
chemical_list: D017931:DNA Primers; D018744:DNA, Plant