title: Interleukin-4 and -8 gene polymorphisms and risk of gastric cancer in a population in Southwestern China.
abstract: BACKGROUND
  Gastric carcinogenesis is a complicated process that involves environmental and genetic factors like interleukin-4 (IL-4) and IL-8. Single nucleotide polymorphisms in their genes are associated with changed levels of gene expression. Here, we investigated the association between IL4-590 C>T and IL8-251T>A and gastric cancer (GC) risk in Sichuan of Southwestern China.
  
  
  METHODS
  We surveyed the research subjects using a self-designed questionnaire with questions on demographic factors and putative risk factors. Approximately 2-5ml of whole blood was collected after field survey to analyze IL4-590 C>T and IL8-251T>A genotypes using MALDI-TOF MS.
  
  
  RESULTS
  Our study recruited 308 pairs of GC patients and controls, including 224 (72.7%) men and 84 (27.3%) women in each group. There were 99 cardia and 176 noncardia GC patients in the case group. The case and control groups had an average age of 57.7±10.6 (mean±SD) and 57.6±11.1 years. GC patients reported a significantly greater proportion of family history of cancer (29.9% vs 10.7%, p<0.01) and drinking (54.6% vs 43.2%, p<0.01) than did controls. Variant genotypes of IL-4-590 C>T and IL-8-251 T>A were not associated with overall GC risk (adjusted OR, 0.89; 95%CI, 0.61-1.28 for CT or CC vs TT; adjusted OR, 1.14; 95%CI, 0.86-1.79 for TA or AA vs TT). Stratification analysis of two SNPs for risk by subsites only found that variant IL-8-251 TA or AA genotype was associated with increased noncardia GC risk (adjusted OR, 2.58; 95%CI, 1.19-5.57). We did not observe interactions between the IL-8-251 T>A genotype and smoking (adjusted OR, 0.38; 95%CI, 0.08-1.79) or drinking (adjusted OR, 0.36; 95%CI, 0.08-1.65) for risk of noncardia GC.
  
  
  CONCLUSIONS
  Our data indicate no association between the two SNPs of IL-4-590 and IL-8-251 with overall GC risk, while the IL-8-251 TA or AA genotype conferred risk of cardia GC. Our findings contribute to the evidence body for risk of SNPs associated with the development of gastric cancer in this region.
authors.affiliation: Department of Epidemiology, West China School of Public Health, Sichuan University, Chengdu, China E-mail : chunxia815@aliyun.com.
authors.forename: Xiong-Fei
authors.initials: XF
authors.lastname: Pan
authors.forename: Ying
authors.initials: Y
authors.lastname: Wen
authors.forename: Marie
authors.initials: M
authors.lastname: Loh
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wen
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Yang
authors.forename: Zhi-Mei
authors.initials: ZM
authors.lastname: Zhao
authors.forename: Zhi
authors.initials: Z
authors.lastname: Tian
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Lan
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Richie
authors.initials: R
authors.lastname: Soong
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Yang
mesh_terms: D002299:Cardia / Q000473:pathology; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D003710:Demography; D005190:Family; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics*; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000453:epidemiology* / Q000235:genetics*; D011795:Surveys and Questionnaires
chemical_list: C508606:CXCL8 protein, human; C508598:IL4 protein, human; D016209:Interleukin-8; D015847:Interleukin-4