title: On-the-fly decoding luminescence lifetimes in the microsecond region for lanthanide-encoded suspension arrays.
abstract: Significant multiplexing capacity of optical time-domain coding has been recently demonstrated by tuning luminescence lifetimes of the upconversion nanoparticles called 'τ-Dots'. It provides a large dynamic range of lifetimes from microseconds to milliseconds, which allows creating large libraries of nanotags/microcarriers. However, a robust approach is required to rapidly and accurately measure the luminescence lifetimes from the relatively slow-decaying signals. Here we show a fast algorithm suitable for the microsecond region with precision closely approaching the theoretical limit and compatible with the rapid scanning cytometry technique. We exploit this approach to further extend optical time-domain multiplexing to the downconversion luminescence, using luminescence microspheres wherein lifetimes are tuned through luminescence resonance energy transfer. We demonstrate real-time discrimination of these microspheres in the rapid scanning cytometry, and apply them to the multiplexed probing of pathogen DNA strands. Our results indicate that tunable luminescence lifetimes have considerable potential in high-throughput analytical sciences.
authors.affiliation: Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University, Sydney, New South Wales 2109, Australia.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University, Sydney, New South Wales 2109, Australia.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.affiliation: Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University, Sydney, New South Wales 2109, Australia.
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Laboratory for Molecular Photonics, University of Miami, 1301 Memorial Drive, Coral Gables, Florida 33146-0431, USA.
authors.forename: Janet
authors.initials: J
authors.lastname: Cusido
authors.affiliation: Laboratory for Molecular Photonics, University of Miami, 1301 Memorial Drive, Coral Gables, Florida 33146-0431, USA.
authors.forename: Françisco M
authors.initials: FM
authors.lastname: Raymo
authors.affiliation: State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116024, China.
authors.forename: Jingli
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Newport Instruments, 3345 Hopi Place, San Diego, California 92117-3516, USA.
authors.forename: Sean
authors.initials: S
authors.lastname: Yang
authors.affiliation: Newport Instruments, 3345 Hopi Place, San Diego, California 92117-3516, USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Leif
authors.affiliation: Department of Electronic Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Huo
authors.affiliation: Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University, Sydney, New South Wales 2109, Australia.
authors.forename: James A
authors.initials: JA
authors.lastname: Piper
authors.affiliation: Purdue University Cytometry Laboratories, Bindley Bioscience Center, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: J
authors.initials: J
authors.lastname: Paul Robinson
authors.affiliation: Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University, Sydney, New South Wales 2109, Australia.
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Goldys
authors.affiliation: 1] Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University, Sydney, New South Wales 2109, Australia [2] Purdue University Cytometry Laboratories, Bindley Bioscience Center, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Dayong
authors.initials: D
authors.lastname: Jin
mesh_terms: D000465:Algorithms; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049449:Luminescence
chemical_list: D028581:Lanthanoid Series Elements