title: p-Coumaric acid and ursolic acid from Corni fructus attenuated β-amyloid(25-35)-induced toxicity through regulation of the NF-κB signaling pathway in PC12 cells.
abstract: Neuroinflammatory responses induced by amyloid-beta peptide (Aβ) are important causes in the pathogenesis of Alzheimer's disease (AD). Blockade of Aβ has emerged as a possible therapeutic approach to control the onset of AD. This study investigated the neuroprotective effects and molecular mechanisms of p-coumaric acid (p-CA) and ursolic acid (UA) from Corni fructus against Aβ(25-35)-induced toxicity in PC12 cells. p-CA and UA significantly inhibited the expression of iNOS and COX-2 in Aβ(25-35)-injured PC12 cells. Blockade of nuclear translocation of the p65 subunit of nuclear factor κB (NF-κB) and phosphorylation of IκB-α was also observed after p-CA and UA treatment. For the upstream kinases, UA exclusively reduced ERK1/2, p-38, and JNK phosphorylation, but p-CA suppressed ERK1/2 and JNK phosphorylation. Both compounds comprehensively inhibited NF-κB activity, but possibly with different upstream pathways. The results provide new insight into the pharmacological modes of p-CA and UA and their potential therapeutic application to AD.
authors.affiliation: Department of Food Science and Nutrition, Dong-A University , Busan 604-714, Korea.
authors.forename: Jeong-Hyun
authors.initials: JH
authors.lastname: Yoon
authors.forename: Kumju
authors.initials: K
authors.lastname: Youn
authors.forename: Chi-Tang
authors.initials: CT
authors.lastname: Ho
authors.forename: Mukund V
authors.initials: MV
authors.lastname: Karwe
authors.forename: Woo-Sik
authors.initials: WS
authors.lastname: Jeong
authors.forename: Mira
authors.initials: M
authors.lastname: Jun
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology; D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D029768:Cornus / Q000737:chemistry*; D003373:Coumaric Acids / Q000494:pharmacology*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D016328:NF-kappa B / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011422:Propionates; D051381:Rats; D014315:Triterpenes / Q000494:pharmacology*; D000097245:Ursolic Acid
chemical_list: D016229:Amyloid beta-Peptides; D003373:Coumaric Acids; D016328:NF-kappa B; D018696:Neuroprotective Agents; D010936:Plant Extracts; D011422:Propionates; D014315:Triterpenes; D000097245:Ursolic Acid; C495469:p-coumaric acid