title: Procalcitonin sensitive detection based on graphene-gold nanocomposite film sensor platform and single-walled carbon nanohorns/hollow Pt chains complex as signal tags.
abstract: Septicemia is a serious disease that requires early diagnosis, and procalcitonin (PCT) serves as a diagnostic biomarker for this disease. Traditional clinical detection (via immune-gold chips) remains difficult and expensive. An electrochemical immunosensor based on new nanomaterials may provide a solving approach. Herein, an ultrasensitive sandwich electrochemical strategy for PCT detection was developed. Firstly, reduced graphene oxide (rGO)-gold (Au) nano-composite film was used as the immunosensor platform to increase the amount of PCT antibody 1(Ab1) immobilized. Next, single-walled carbon nanohorns (SWCNHs)/hollow Pt chains (HPtCs) complex was firstly utilized to label PCT Ab2 as signal tags. For SWCNHs with few side effects, high surface area and HPtCs with higher specific surface, better catalytic activity, complex synthesized from both may provide more advantages. Moreover, to amplify signal, HPtC catalytic activity with H2O2 was enhanced by horseradish peroxidase (HRP) for dual synergy amplification. The whole results demonstrated that the proposed immunosensor exhibited fast operation, high sensitivity, good reproducibility, acceptable stability and ideal selectivity compared with traditional method. The linear calibration of the immunosensor ranged from 1.00 pg/mL to 2.00 × 10(1)ng/mL with a detection limit of 0.43 pg/mL. Analytical application results revealed that the immunosensor matched with the real concentrations of serum samples. Overall this immunosensor may provide a new alternative strategy for PCT detection.
authors.affiliation: Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Guiming
authors.initials: G
authors.lastname: Xiang
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Southwest University, Chongqing 400715, China.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.affiliation: Shima River Community Health Center, Jiangbei District, Chongqing 400021, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Fukang
authors.initials: F
authors.lastname: Luo
authors.affiliation: Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Dongneng
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Shaoguang
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. Electronic address: puxiaoyong@yahoo.com.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Pu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D002116:Calcitonin / Q000032:analysis* / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D058608:Nanopores / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010984:Platinum / Q000737:chemistry; D011498:Protein Precursors / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D008567:Membranes, Artificial; D037742:Nanotubes, Carbon; D011498:Protein Precursors; D010984:Platinum; D006046:Gold; D006108:Graphite; D002116:Calcitonin
keywords: HPtCs; Immunosensor; Procalcitonin (PCT); Reduced graphene oxide (rGO); SWCNHs