title: Transformation and measurement of bioluminescence rhythms in the moss Physcomitrella patens.
abstract: Gene targeting is a highly effective and straightforward technique for the functional analysis of a gene of interest. However, its efficiency is not satisfactorily high in many model plants including Arabidopsis thaliana. In the moss Physcomitrella patens, a model species of basal plants, the efficiency of gene targeting is as high as in yeasts, and this moss is becoming widely recognized as an experimental model of choice in various areas of plant biology. Here we focus on the transformation of protoplast cells and on the measurement of bioluminescence rhythms from protonema tissues of luciferase reporter strains in P. patens, both of which are important for mechanistic studies of the circadian clock.
authors.affiliation: Graduate School of Information Science, Nagoya University Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan, aoki@is.nagoya-u.ac.jp.
authors.forename: Setsuyuki
authors.initials: S
authors.lastname: Aoki
authors.forename: Ryo
authors.initials: R
authors.lastname: Okada
authors.forename: Santosh B
authors.initials: SB
authors.lastname: Satbhai
mesh_terms: D019068:Bryopsida / Q000502:physiology*; D002940:Circadian Rhythm / Q000502:physiology*; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D008164:Luminescent Proteins