title: Metabolomics analysis of collagen-induced arthritis in rats and interventional effects of oral tolerance.
abstract: A serum metabolomics method based on rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (RRLC-Q-TOF-MS) was performed for a holistic evaluation of the metabolic changes of collagen-induced arthritis (CIA) in rats and to assess the interventional effects of type II collagen (CII) in this model. Partial least-squares-discriminant analysis (PLS-DA) was employed to study the metabolic profiling of CIA rats and control rats. Ten metabolites, namely, 12(S)-HHTrE, 12(S)-HEPE, PGE2, TXB2, 12(S)-HETE, LysoPE(16:0), PE(O-18:0/0:0), Lyso-PE(18:2), Lyso-PE(20:4), and Lyso-PC(22:5) were identified as differential metabolites associated with the pathogenesis of CIA. These results suggested that dysregulation of the arachidonic acid (AA) and phospholipid metabolic networks is involved in the pathomechanism of CIA. Differential metabolomics and histopathological analyses demonstrated that CII inhibits the progress of arthritis. Furthermore, the therapeutic effects of CII on CIA may involve regulation of the disordered AA and phospholipid metabolic networks. This metabolomics study provides new insights into the pathogenesis of arthritis and, furthermore, indicates the potential mechanism underlying the significantly increased prevalence of metabolic syndrome, defined as a clustering of cardiovascular disease (CVD) risk factors, in arthritis patients.
authors.affiliation: College of Basic Medical, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Xinghong
authors.initials: X
authors.lastname: Ding
authors.affiliation: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Shui
authors.affiliation: College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Zhishan
authors.initials: Z
authors.lastname: Ding
authors.affiliation: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yao
authors.affiliation: College of Basic Medical, Zhejiang Chinese Medical University, Hangzhou, China. Electronic address: applezjtcm@126.com.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Fan
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis; D001169:Arthritis, Experimental / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D024043:Collagen Type II / Q000627:therapeutic use; D016002:Discriminant Analysis; D004195:Disease Models, Animal; D016018:Least-Squares Analysis; D008297:Male; D055442:Metabolome; D055432:Metabolomics; D010743:Phospholipids / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D024043:Collagen Type II; D010743:Phospholipids; D016718:Arachidonic Acid
keywords: Collagen-induced arthritis; Metabolomics; PLS-DA; RRLC-Q-TOF-MS; Rheumatoid arthritis