title: Transposon mutagenesis.
abstract: Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we present a detailed protocol for using Himar1 mariner-based transposon mutagenesis to create mutant libraries in P. aeruginosa.
authors.affiliation: Department of Microbiology, University of Washington, 1959 NE Pacific St HSB K161, Seattle, WA, 98195, USA, donhk@uw.edu.
authors.forename: Hemantha D
authors.initials: HD
authors.lastname: Kulasekara
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D005822:Genetic Vectors / Q000378:metabolism; D016254:Mutagenesis, Insertional / Q000379:methods*; D009154:Mutation / Q000235:genetics; D059372:Nucleotide Motifs / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA
chemical_list: D004251:DNA Transposable Elements