title: Genomic variation in Arabidopsis: tools and insights from next-generation sequencing.
abstract: The release of a reference genome for Arabidopsis thaliana in 2000 has been an enormous boon for the study of plant genetics. Less than a decade later, however, a revolution in sequencing technology had enabled rapid and inexpensive re-sequencing of whole A. thaliana genomes. Large-scale efforts to characterize natural genomic variation in A. thaliana have revealed remarkable intra-specific variation in this species, ranging from single-nucleotide differences to large structural rearrangements. The partitioning of this variation by geography and local adaptation has been described using powerful new methods and tools. Simultaneously, an ambitious research agenda has emerged to sequence 1001 A. thaliana lines from around the world, while sequencing of related species is enabling powerful evolutionary genomic analyses. In this review, I summarize recent progress in genomic analysis of natural variation in A. thaliana and its close relatives. This progress has set the stage for the emergence of Arabidopsis as a model genus for evolutionary and functional genomics.
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, ON, M5S 1G8, Canada, jesse.hollister@utoronto.ca.
authors.forename: Jesse D
authors.initials: JD
authors.lastname: Hollister
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D057890:Epigenomics; D019143:Evolution, Molecular; D018745:Genome, Plant; D023281:Genomics / Q000379:methods*; D006239:Haplotypes; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D058974:Phylogeography; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant