title: Absorption, distribution and pathological injury in mice due to ricin poisoning via the alimentary pathway.
abstract: The aim of this work was to investigate the potential interactions between intestinal absorbance and ricin poisoning. The Caco-2 cell monolayer and everted intestinal sac (VEIS) models were used. The distribution of ricin in CD-1 mice intoxicated with 0.1 mg/kg of ricin intragastrically was determined by immunohistochemistry. The results showed that ricin could not transfer across the healthy Caco-2 cell monolayer within three hours after poisoning. However, it could pass through the everted rat intestinal wall after 0.5 h of incubation. The toxin in the liver, spleen, lungs and kidneys of mice could be detected as early as 1 h after intoxication. The pathological results were in accordance with the cytotoxicities of ricin in Caco-2, HepG 2, H1299 and MDCK cells, indicating that though no significant symptom in mice could be observed within 3 h after ricin intoxication, important tissues, especially the kidneys, were being injured by the toxin and that the injuries were progressing.
authors.affiliation: General Hospital of Beijing Military Command, Beijing 100700, China ; Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Na
authors.initials: N
authors.lastname: Dong
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Wu
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Jia
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Zhongcai
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Han
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China ; School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Shan
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Wei
keywords: alimentary poisoning; immunohistochemistry; ricin