title: A simple, sensitive colorimetric assay for coralyne based on target induced split G-quadruplex formation.
abstract: The screening of potential drugs specifically binding to polydeoxyadenosine (poly(dA)) has been of great interest in recent studies. We have developed a simple colorimetric strategy through the mechanism of target induced split G-quadruplex formation for detecting coralyne, a poly(dA)-binding drug with noticeable antitumor activity. Two DNA oligonucleotides containing a split G-quadruplex sequence and an adenine-rich sequence are used in our strategy. In the presence of coralyne, the adenine-rich sequences of two oligonucleotides are drawn into close proximity, resulting in the formation of a split G-quadruplex DNAzyme that catalyzes the generation of a colored product. The DNAzyme-based colorimetric assay for coralyne has a linear range of from 0.033 to 1.667 μM with a low detection limit of 16 nM. The developed method is simple, cost-effective and visible; it holds great potential for applications in drug screening.
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University.
authors.forename: Yingya
authors.initials: Y
authors.lastname: Kan
authors.forename: Cheng
authors.initials: C
authors.lastname: Jiang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xi
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
authors.forename: Jianhui
authors.initials: J
authors.lastname: Jiang
authors.forename: Ruqin
authors.initials: R
authors.lastname: Yu
mesh_terms: D001600:Berberine Alkaloids / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002942:Circular Dichroism; D003124:Colorimetry / Q000379:methods; D021881:DNA, Catalytic / Q000737:chemistry*; D054856:G-Quadruplexes; D011061:Poly A / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044366:Transition Temperature
chemical_list: D001600:Berberine Alkaloids; D021881:DNA, Catalytic; D011061:Poly A; C015465:poly(dA); C000666:coralyne