title: Oxidative potential of ultraviolet-A irradiated or nonirradiated suspensions of titanium dioxide or silicon dioxide nanoparticles on Allium cepa roots.
abstract: The effect of ultraviolet-A irradiated or nonirradiated suspensions of agglomerates of titanium dioxide (TiO(2)) or silicon dioxide (SiO(2)) nanoparticles on roots of the onion (Allium cepa) has been studied. The reactive potential of TiO(2) nanoparticles, which have photocatalytic potential, and the nonphotocatalytic SiO(2) nanoparticles with the same size of agglomerates was compared. The authors measured the activity of antioxidant enzymes glutathione reductase, ascorbate peroxidase, guaiacol peroxidase, and catalase as well as lipid peroxidation to assess the oxidative stress in exposed A. cepa roots. A wide range of concentrations of nanoparticles was tested (0.1-1000 µg/mL). The sizes of agglomerates ranged in both cases from 300 nm to 600 nm, and the exposure time was 24 h. Adsorption of SiO(2) nanoparticles on the root surface was minimal but became significant when roots were exposed to TiO(2) agglomerates. No significant biological effects were observed even at high exposure concentrations of SiO(2) and TiO(2) nanoparticles individually. Plants appear to be protected against nanoparticles by the cell wall, which shields the cell membrane from direct contact with the nanoparticles. The authors discuss the need to supplement conventional phytotoxicity and stress end points with measures of plant physiological state when evaluating the safety of nanoparticles.
authors.forename: Jasna Dolenc
authors.initials: JD
authors.lastname: Koce
authors.forename: Damjana
authors.initials: D
authors.lastname: Drobne
authors.forename: Katja
authors.initials: K
authors.lastname: Klančnik
authors.forename: Darko
authors.initials: D
authors.lastname: Makovec
authors.forename: Sara
authors.initials: S
authors.lastname: Novak
authors.forename: Matej
authors.initials: M
authors.lastname: Hočevar
mesh_terms: D060387:Ascorbate Peroxidases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D053758:Nanoparticles / Q000528:radiation effects / Q000633:toxicity*; D019697:Onions / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D012822:Silicon Dioxide / Q000528:radiation effects / Q000633:toxicity*; D014025:Titanium / Q000528:radiation effects / Q000633:toxicity*; D014466:Ultraviolet Rays
chemical_list: D010940:Plant Proteins; C009495:titanium dioxide; D012822:Silicon Dioxide; D014025:Titanium; C121048:guaiacol peroxidase; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D005980:Glutathione Reductase