title: New method for isolation of rat lamina propria macrophages in colonic tissue.
abstract: Studies on intestinal cells in the lamina propria are important for understanding the cellular and immune responses in the gut. There is a lack of specific isolating procedures of macrophage cells in rats. Two different procedures of macrophage isolation of the lamina propria in rats are compared: a standard mice protocol for lymphocyte isolation (A) adapted to rat samples and a new protocol developed specifically for rats (B). Significant differences are observed when analyzing the effect of the isolation method on the cell number, viability and phenotype. This has important implications when further functional studies are required.
authors.affiliation: Gastroenterology Department, Hospital De Sant Joan Despí Moisès Broggi, Sant Joan Despí, Barcelona, Spain; Germans Trias i Pujol Foundation (IGTP), Badalona, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Bargalló
authors.affiliation: Germans Trias i Pujol Foundation (IGTP), Badalona, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Abad
authors.affiliation: Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; CIBERehd, Ciber de Enfermedades Hepaticas y Digestivas, Barcelona, Spain; Department of Nutrition, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Gemma
authors.initials: G
authors.lastname: Odena
authors.affiliation: CIBERehd, Ciber de Enfermedades Hepaticas y Digestivas, Barcelona, Spain; Hepatology Unit, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain.
authors.forename: Ramon
authors.initials: R
authors.lastname: Planas
authors.affiliation: Germans Trias i Pujol Foundation (IGTP), Badalona, Spain; CIBERehd, Ciber de Enfermedades Hepaticas y Digestivas, Barcelona, Spain. Electronic address: rbartolisole@gmail.com.
authors.forename: Ramon
authors.initials: R
authors.lastname: Bartolí
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D003106:Colon / Q000166:cytology*; D007413:Intestinal Mucosa / Q000166:cytology*; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D008297:Male; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D039481:CD11b Antigen
keywords: CD11b cells; Isolation method; Lamina propria; Macrophages; Rats