title: [Effects on Salvia miltiorrhiza hairy roots of tanshinones content accumulation after treated with fosmidomycin].
abstract: Fosmidomycin (100 micromol x L(-1)) which is the effective inhibitor of DXR, key enzyme in terpenoid MEP pathway, was used to treat with hairy roots of Salvia miltiorrhiza. The treated roots were harvested at 2, 4, 6, 8, 10, 16 and 21 d, mRNA level of SmDXR and tanshinone content in treated and negative control groups were detected. Results found that, after treated with fosmidomycin, color of S. miltiorrhiza hairy roots grew pale gradually comparing with controls; mRNA level of SmDXR in hairy roots varied as a shape of parabolic and the highest value achieved at the sixth day after treatment, then it decreased gradually; Content of four kinds of tanshinones were detected. Among of the four kinds of tanshinones, Tanshinone I content changed relatively little, while content of dihydrotanshinone I, cryptotanshinone and tanshinone II (A) decreased gradually in 21 days. The content of total tanshinones in NC groups was 5, 63 times more than FOS-treated roots in the 21th day. The previous results showed that SmDXR played an important role in the accumulation of tanshinone content in MEP pathway. Once the mRNA level of SmDXR was suppressed, the accumulation of secondary metabolites will be significantly affected.
authors.forename: Xia-Nan
authors.initials: XN
authors.lastname: Zhang
authors.forename: Zhi-Shuang
authors.initials: ZS
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
mesh_terms: D045784:Abietanes / Q000378:metabolism*; D019747:Aldose-Ketose Isomerases / Q000235:genetics; D005578:Fosfomycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
chemical_list: D045784:Abietanes; D012333:RNA, Messenger; C021751:tanshinone; D005578:Fosfomycin; C024640:fosmidomycin; C114231:1-deoxy-D-xylulose 5-phosphate reductoisomerase; D019747:Aldose-Ketose Isomerases