title: Macromolecular depletion as a determinant of RBC adhesive interactions: why blood is thicker than water.
abstract: If a surface is in contact with a solution containing macromolecules or proteins, and the loss of configurational entropy of these molecules at the surface is not balanced by adsorption energy, a polymer-poor layer will develop near the surface. If two such layers overlap, an attractive force develops due to the osmotic pressure difference between these depletion zones and the bulk phase. Recent studies have shown that depletion interaction plays a major role in red blood cell (RBC) aggregation and hence it is a major determinate of blood flow stability; depletion interaction also markedly affects RBC adhesion to vascular endothelial cells. Understanding and quantitating factors that regulate depletion in vivo are thus of importance, yet made difficult since only very small changes of the cell surface (e.g., glycocalyx thickness) such as seen during RBC aging can lead to massive changes of depletion interaction and hence cell-cell adhesion. It is suggested that insight into the in vivo relevance of depletion mechanisms may lead to an improved understanding of how and why blood flow is altered in many diseases, and may also provide new biomarkers (e.g., surface properties) that will aid in the development of novel or improved diagnostic and therapeutic tools.
authors.affiliation: Faculty of Life Sciences, University of Applied Sciences Rhine-Waal, Kleve, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Neu
authors.affiliation: Department of Physiology and Biophysics, Keck School of Medicine, Los Angeles, CA, USA.
authors.forename: Herbert J
authors.initials: HJ
authors.lastname: Meiselman
mesh_terms: D002448:Cell Adhesion; D004903:Erythrocyte Aggregation / Q000502:physiology*; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans
keywords: Aggregation; adhesion; cell affinity; polymer depletion