title: Fe deficiency induces phosphorylation and translocation of Lhcb1 in barley thylakoid membranes.
abstract: HvLhcb1 a major light-harvesting chlorophyll a/b-binding protein in barley, is a critical player in sustainable growth under Fe deficiency. Here, we demonstrate that Fe deficiency induces phosphorylation of HvLhcb1 proteins leading to their migration from grana stacks to stroma thylakoid membranes. HvLhcb1 remained phosphorylated even in the dark and apparently independently of state transition, which represents a mechanism for short-term acclimation. Our data suggest that the constitutive phosphorylation-triggered translocation of HvLhcb1 under Fe deficiency contributes to optimization of the excitation balance between photosystem II and photosystem I, the latter of which is a main target of Fe deficiency.
authors.affiliation: Department of Applied Biology and Chemistry, Tokyo University of Agriculture, Tokyo 156-8502, Japan; Faculty of Education and Integrated Arts and Sciences, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan. Electronic address: a3saito@nodai.ac.jp.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Saito
authors.affiliation: Department of Applied Biology and Chemistry, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
authors.forename: Mizuho
authors.initials: M
authors.lastname: Shimizu
authors.affiliation: Department of Applied Biology and Chemistry, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Nakamura
authors.affiliation: Department of Applied Biology and Chemistry, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Maeno
authors.affiliation: Department of Applied Biology and Chemistry, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
authors.forename: Riko
authors.initials: R
authors.lastname: Katase
authors.affiliation: Department of Applied Biology and Chemistry, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
authors.forename: Eitaro
authors.initials: E
authors.lastname: Miwa
authors.affiliation: Department of Applied Biology and Chemistry, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Higuchi
authors.affiliation: Faculty of Education and Integrated Arts and Sciences, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan.
authors.forename: Kintake
authors.initials: K
authors.lastname: Sonoike
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D001467:Hordeum / Q000166:cytology* / Q000378:metabolism*; D000090463:Iron Deficiencies; D010766:Phosphorylation; D055668:Photochemical Processes; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D021381:Protein Transport; D020524:Thylakoids / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll
keywords: Barley; Iron deficiency; Light-harvesting antenna complex II; Non-photochemical quenching; Photosynthetic electron transport; Protein phosphorylation