title: Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins.
abstract: A traceable linker that is potentially applicable to identification of a target protein of bioactive compounds was developed. It enabled not only thiol-induced cleavage of the linker for enrichment of the target protein but also selective labelling to pick out the target from contaminated non-target proteins for facile identification.
authors.affiliation: Institute of Health Biosciences and Graduate School of Pharmaceutical Sciences, The University of Tokushima, Shomachi, Tokushima 770-8505, Japan. shigenaga.akira@tokushima-u.ac.jp aotaka@tokushima-u.ac.jp.
authors.forename: Jun
authors.initials: J
authors.lastname: Yamamoto
authors.forename: Masaya
authors.initials: M
authors.lastname: Denda
authors.forename: Nami
authors.initials: N
authors.lastname: Maeda
authors.forename: Miku
authors.initials: M
authors.lastname: Kita
authors.forename: Chiaki
authors.initials: C
authors.lastname: Komiya
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Tanaka
authors.forename: Wataru
authors.initials: W
authors.lastname: Nomura
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tamamura
authors.forename: Youichi
authors.initials: Y
authors.lastname: Sato
authors.forename: Aiko
authors.initials: A
authors.lastname: Yamauchi
authors.forename: Akira
authors.initials: A
authors.lastname: Shigenaga
authors.forename: Akira
authors.initials: A
authors.lastname: Otaka
mesh_terms: D000577:Amides / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry*; D057930:Click Chemistry / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D013194:Staining and Labeling; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000577:Amides; D000596:Amino Acids; D010455:Peptides; D011506:Proteins; D013438:Sulfhydryl Compounds