title: MicroRNA targeting of CoREST controls polarization of migrating cortical neurons.
abstract: The migration of cortical projection neurons is a multistep process characterized by dynamic cell shape remodeling. The molecular basis of these changes remains elusive, and the present work describes how microRNAs (miRNAs) control neuronal polarization during radial migration. We show that miR-22 and miR-124 are expressed in the cortical wall where they target components of the CoREST/REST transcriptional repressor complex, thereby regulating doublecortin transcription in migrating neurons. This molecular pathway underlies radial migration by promoting dynamic multipolar-bipolar cell conversion at early phases of migration, and later stabilization of cell polarity to support locomotion on radial glia fibers. Thus, our work emphasizes key roles of some miRNAs that control radial migration during cerebral corticogenesis.
authors.affiliation: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Volvert
authors.affiliation: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Pierre-Paul
authors.initials: PP
authors.lastname: Prévot
authors.affiliation: GIGA-Signal Transduction, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Close
authors.affiliation: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Sophie
authors.initials: S
authors.lastname: Laguesse
authors.affiliation: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Sophie
authors.initials: S
authors.lastname: Pirotte
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA; Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: James
authors.initials: J
authors.lastname: Hemphill
authors.affiliation: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Florence
authors.initials: F
authors.lastname: Rogister
authors.affiliation: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Kruzy
authors.affiliation: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Rosalie
authors.initials: R
authors.lastname: Sacheli
authors.affiliation: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Gustave
authors.initials: G
authors.lastname: Moonen
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA; Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Deiters
authors.affiliation: Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK.
authors.forename: Matthias
authors.initials: M
authors.lastname: Merkenschlager
authors.affiliation: GIGA-Signal Transduction, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Walloon Excellence in Lifesciences and Biotechnology (WELBIO), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Alain
authors.initials: A
authors.lastname: Chariot
authors.affiliation: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Malgrange
authors.affiliation: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
authors.forename: Juliette D
authors.initials: JD
authors.lastname: Godin
authors.affiliation: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Walloon Excellence in Lifesciences and Biotechnology (WELBIO), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium. Electronic address: lnguyen@ulg.ac.be.
authors.forename: Laurent
authors.initials: L
authors.lastname: Nguyen
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D056970:Co-Repressor Proteins; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D000099229:RE1-Silencing Transcription Factor
chemical_list: D056970:Co-Repressor Proteins; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D012097:Repressor Proteins; D000099229:RE1-Silencing Transcription Factor; C540079:Mirn124 microRNA, mouse; C574876:Mirn22 microRNA, mouse; C485650:Rcor2 protein, mouse