title: Pectin methylesterase-generated methanol may be involved in tobacco leaf growth.
abstract: Plant leaves undergo a sink-source modification of intercellular macromolecular transport during the transition from carbon import to carbon export. After assessing the role of metabolite signaling in gene regulation in Nicotiana tabacum sink and source leaves, we observed increased pectin methylesterase (PME)-mediated methanol generation in immature leaves. Using suppression subtractive hybridization (SSH), we identified a number of genes whose activity changes from sink to source leaves. The most abundant SSH-identified genes appeared to be sensitive to methanol. We hypothesize that tobacco leaf maturation and the sink-source transition are accompanied by a change in mRNA levels of genes that function in methanol-dependent cell signaling.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia. dorokhov@genebee.msu.su.
authors.forename: T V
authors.initials: TV
authors.lastname: Komarova
authors.forename: D V
authors.initials: DV
authors.lastname: Pozdyshev
authors.forename: I V
authors.initials: IV
authors.lastname: Petrunia
authors.forename: E V
authors.initials: EV
authors.lastname: Sheshukova
authors.forename: Y L
authors.initials: YL
authors.lastname: Dorokhov
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism*; D018515:Plant Leaves / Q000254:growth & development* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D000432:Methanol