title: Amelioration of streptozotocin-induced diabetic nephropathy by melatonin, quercetin, and resveratrol in rats.
abstract: The role of oxygen radicals are known for the pathogenesis of kidney damage. The aim of the present study was to investigate the antioxidative effects of melatonin, quercetin, and resveratrol on streptozotocin (STZ)-induced diabetic nephropathy in rats. A total of 35 male Wistar rats were divided into 5 groups as follows: control, diabetes mellitus (DM), DM + melatonin, DM + quercetin, and DM + resveratrol. All the injections started on the same day of single-dose STZ injection and continued for 30 days. At the end of this period, kidneys were removed and processed for routine histological procedures. Biochemical parameters and morphological changes were examined. In DM group, blood glucose levels were significantly increased, whereas body weights were decreased compared with the control group. Significant increases in blood urea nitrogen and tissue malondialdehyde (MDA) levels and decreases in superoxide dismutase and catalase activities were detected in DM group. Administration of melatonin, quercetin, and resveratrol significantly reduced these values. Melatonin was more efficient in reducing MDA levels than other antioxidants (p < 0.05). STZ-induced histopathological alterations including epithelial desquamation, swelling, intracytoplasmic vacuolization, brush border loss and peritubular infiltration. Additionally, basement membrane thickening and sclerotic changes were observed in glomerulus. Transforming growth factor-β1 positive cells were also increased. Melatonin, quercetin, and resveratrol significantly reduced these histopathological changes. Our results indicate that melatonin, quercetin, and resveratrol might be helpful in reducing diabetes-induced renal damage.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Inonu University, Malatya, Turkey h_elbe@hotmail.com.
authors.forename: H
authors.initials: H
authors.lastname: Elbe
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Inonu University, Malatya, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Vardi
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Esrefoglu
authors.affiliation: Department of Chemistry, Faculty of Science and Art, Inonu University, Malatya, Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Ates
authors.affiliation: Department of Biostatistics, Faculty of Medicine, Inonu University, Malatya, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Yologlu
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Inonu University, Malatya, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Taskapan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001786:Blood Glucose / Q000032:analysis; D001806:Blood Urea Nitrogen; D002374:Catalase / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology / Q000627:therapeutic use*; D011794:Quercetin / Q000494:pharmacology / Q000627:therapeutic use*; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D013267:Stilbenes; D008315:Malondialdehyde; D011794:Quercetin; D003404:Creatinine; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D008550:Melatonin; D000077185:Resveratrol
keywords: Streptozotocin; TGF-β1; diabetic nephropathy; melatonin; quercetin; resveratrol