title: [Sequence homology analysis on lactate dehydrogenase (LDH) of Plasmodium vivax Anhui isolates].
abstract: DNA in dried blood spots of 39 vivax malaria patients (2009-2010) from Anhui (Bengbu urban district and counties of Wuhe, Huaiyuan, Mengcheng and Lixin) was extracted. The Plasmodium vivax LDH (PvLDH) gene was amplified, cloned and sequenced. The sequences were subjected to NCBI Blast program. The results showed that the targeted DNA fragment size was 951 bp without difference among the 39 samples (accession No. GU078391), and was more than 99% homologous to the PvLDH sequences in other strains from GenBank. There was only one different amino acid in the protein sequences between the isolates from Anhui and EJEU60134 or MIA061251 strains.
authors.forename: Ming-Jie
authors.initials: MJ
authors.lastname: Hu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fang
authors.forename: Shou-Wei
authors.initials: SW
authors.lastname: Wu
authors.forename: Bi-Kui
authors.initials: BK
authors.lastname: Tang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yu-Meng
authors.initials: YM
authors.lastname: Jiao
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Shen
mesh_terms: D002681:China; D016054:DNA, Protozoan / Q000235:genetics; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D010966:Plasmodium vivax / Q000201:enzymology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017386:Sequence Homology, Amino Acid
chemical_list: D016054:DNA, Protozoan; D007770:L-Lactate Dehydrogenase