title: Nonomuraea muscovyensis sp. nov., isolated from soil.
abstract: A novel actinomycete, strain FMN03(T), was isolated from a soil sample collected from Yuga Zapadnaya South-West Forest Park, Moscow, Russia. The isolate had chemical and morphological properties typical of members of the genus Nonomuraea and formed a distinct 16S rRNA gene subclade with the type strains Nonomuraea roseoviolacea subsp. carminata NBRC 15903(T) and Nonomuraea roseoviolacea subsp. roseoviolacea NBRC 14098(T). The organism formed extensively branched substrate and aerial hyphae, which generated spiral chains of spores with smooth surfaces. The cell wall contained meso-diaminopimelic acid and the whole cell sugars were glucose, galactose and trace amounts of madurose, mannose and xylose. The polar lipids were phosphatidylethanolamine, hydroxyphosphatidylethanolamine, four unidentified phospholipids, four unidentified glycolipids and one unidentified lipid. The predominant menaquinone was MK-9(H4). The major fatty acids were iso-C16 : 0 2-OH, C17 : 0 10-methyl, C17 : 1 cis9 and iso-C16 : 0. Analyses of its morphological, physiological and biochemical characteristics, together with DNA-DNA relatedness data, confirmed that strain FMN03(T) is a representative of a novel species of the genus Nonomuraea, which is distinct from closely related reference strains. Strain FMN03(T) ( = DSM 45913(T) = KCTC 29233(T)) is proposed as the type strain of a novel species, for which the name Nonomuraea muscovyensis sp. nov. is proposed.
authors.affiliation: Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, Samsun, Turkey.|Department of Nursing, School of Health, Bilecik Seyh Edebali University, Bilecik, Turkey.
authors.forename: Fadime
authors.initials: F
authors.lastname: Ozdemir-Kocak
authors.affiliation: Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Kamil
authors.initials: K
authors.lastname: Isik
authors.affiliation: Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Veyisoglu
authors.affiliation: Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Tatar
authors.affiliation: Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Nevzat
authors.initials: N
authors.lastname: Sahin
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid