title: Immunohistochemical expression of cyclooxygenase-2 in normal, hyperplastic and neoplastic canine lymphoid tissues.
abstract: There is much interest in the potential use of selective inhibitors of cyclooxygenase (COX)-2 in combination with other cancer therapeutics. COX-2 is a key enzyme in prostaglandin synthesis and has been implicated in the pathogenesis of numerous canine and feline malignancies. There are few data on the potential role of COX-2 in the pathogenesis of canine lymphoma. The present study examined COX-2 expression in normal, hyperplastic and neoplastic canine lymphoid tissues. Immunohistochemical expression was evaluated in 12 samples of non-pathologically enlarged normal lymph nodes, 24 samples of hyperplastic lymph node and 44 samples of lymphoma (22 B-cell and 22 T-cell lymphomas). The labelling was scored semiquantitatively and a score of +2 or +3 was interpreted as overexpression. In hyperplastic lymph nodes only a few macrophages were COX-2-positive while six of the 44 lymphomas (13.6%; three B- and three T-cell lymphomas) overexpressed COX-2. These data provide a rationale for further investigation of COX-2 expression in canine lymphoma for prognostic, chemopreventive and chemotherapeutic purposes.
authors.affiliation: Dipartimento di Scienze Veterinarie, University of Pisa, Pisa I-56124, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Asproni
authors.affiliation: Centro Oncologico Veterinario, Via San Lorenzo 1/4, 40037 Sasso Marconi, Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Vignoli
authors.affiliation: Centro Oncologico Veterinario, Via San Lorenzo 1/4, 40037 Sasso Marconi, Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Cancedda
authors.affiliation: Dipartimento di Scienze Veterinarie, University of Pisa, Pisa I-56124, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Millanta
authors.affiliation: Centro Oncologico Veterinario, Via San Lorenzo 1/4, 40037 Sasso Marconi, Bologna, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Terragni
authors.affiliation: Dipartimento di Scienze Veterinarie, University of Pisa, Pisa I-56124, Italy. Electronic address: apoli@vet.unipi.it.
authors.forename: A
authors.initials: A
authors.lastname: Poli
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D006965:Hyperplasia / Q000378:metabolism / Q000662:veterinary; D007150:Immunohistochemistry; D008221:Lymphoid Tissue / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism / Q000662:veterinary*; D008297:Male
chemical_list: D051546:Cyclooxygenase 2
keywords: COX-2; dog; lymphoid tissue; lymphoma