title: Interneuron precursor transplants in adult hippocampus reverse psychosis-relevant features in a mouse model of hippocampal disinhibition.
abstract: GABAergic interneuron hypofunction is hypothesized to underlie hippocampal dysfunction in schizophrenia. Here, we use the cyclin D2 knockout (Ccnd2(-/-)) mouse model to test potential links between hippocampal interneuron deficits and psychosis-relevant neurobehavioral phenotypes. Ccnd2(-/-) mice show cortical PV(+) interneuron reductions, prominently in hippocampus, associated with deficits in synaptic inhibition, increased in vivo spike activity of projection neurons, and increased in vivo basal metabolic activity (assessed with fMRI) in hippocampus. Ccnd2(-/-) mice show several neurophysiological and behavioral phenotypes that would be predicted to be produced by hippocampal disinhibition, including increased ventral tegmental area dopamine neuron population activity, behavioral hyperresponsiveness to amphetamine, and impairments in hippocampus-dependent cognition. Remarkably, transplantation of cells from the embryonic medial ganglionic eminence (the major origin of cerebral cortical interneurons) into the adult Ccnd2(-/-) caudoventral hippocampus reverses these psychosis-relevant phenotypes. Surviving neurons from these transplants are 97% GABAergic and widely distributed within the hippocampus. Up to 6 mo after the transplants, in vivo hippocampal metabolic activity is lowered, context-dependent learning and memory is improved, and dopamine neuron activity and the behavioral response to amphetamine are normalized. These findings establish functional links between hippocampal GABA interneuron deficits and psychosis-relevant dopaminergic and cognitive phenotypes, and support a rationale for targeting limbic cortical interneuron function in the prevention and treatment of schizophrenia.
authors.affiliation: New York State Psychiatric Institute, New York, NY 10032;Department of Biological Sciences, Columbia University, New York, NY 10027; Departments of.
authors.forename: Ahmed I
authors.initials: AI
authors.lastname: Gilani
authors.affiliation: New York State Psychiatric Institute, New York, NY 10032;Psychiatry and.
authors.forename: Muhammad O
authors.initials: MO
authors.lastname: Chohan
authors.affiliation: Department of Psychiatry andFeil Family Brain and Mind Research Institute, Weill Cornell Medical College, New York, NY 10065.
authors.forename: Melis
authors.initials: M
authors.lastname: Inan
authors.affiliation: New York State Psychiatric Institute, New York, NY 10032;Psychiatry and.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Schobel
authors.affiliation: New York State Psychiatric Institute, New York, NY 10032;
authors.forename: Nashid H
authors.initials: NH
authors.lastname: Chaudhury
authors.affiliation: New York State Psychiatric Institute, New York, NY 10032;
authors.forename: Samuel
authors.initials: S
authors.lastname: Paskewitz
authors.affiliation: New York State Psychiatric Institute, New York, NY 10032;Psychiatry and.
authors.forename: Nao
authors.initials: N
authors.lastname: Chuhma
authors.affiliation: Feil Family Brain and Mind Research Institute, Weill Cornell Medical College, New York, NY 10065.
authors.forename: Sara
authors.initials: S
authors.lastname: Glickstein
authors.affiliation: New York State Psychiatric Institute, New York, NY 10032;
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Merker
authors.affiliation: Department of Psychiatry and.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Neurology, Columbia University, New York, NY 10032; and.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Small
authors.affiliation: Department of Psychiatry and hm2035@columbia.edu andersons3@email.chop.edu mer2005@med.cornell.edu.
authors.forename: Stewart A
authors.initials: SA
authors.lastname: Anderson
authors.affiliation: Feil Family Brain and Mind Research Institute, Weill Cornell Medical College, New York, NY 10065 hm2035@columbia.edu andersons3@email.chop.edu mer2005@med.cornell.edu.
authors.forename: Margaret Elizabeth
authors.initials: ME
authors.lastname: Ross
authors.affiliation: New York State Psychiatric Institute, New York, NY 10032;Psychiatry and hm2035@columbia.edu andersons3@email.chop.edu mer2005@med.cornell.edu.
authors.forename: Holly
authors.initials: H
authors.lastname: Moore
mesh_terms: D000818:Animals; D003072:Cognition Disorders / Q000503:physiopathology; D056742:Cyclin D2 / Q000235:genetics; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D005239:Fear; D005260:Female; D006624:Hippocampus / Q000196:embryology* / Q000378:metabolism; D007395:Interneurons / Q000166:cytology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009433:Neural Inhibition; D009473:Neuronal Plasticity / Q000502:physiology; D010320:Parvalbumins / Q000378:metabolism; D011618:Psychotic Disorders / Q000503:physiopathology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology
chemical_list: C486196:Ccnd2 protein, mouse; D056742:Cyclin D2; D010320:Parvalbumins; D004298:Dopamine
keywords: contextual fear conditioning; functional magnetic resonance imaging; neural stem cell therapy; parvalbumin; temporal lobe-dependent cognition