title: The screening research of anti-inflammatory bioactive markers from different flowering phases of Flos Lonicerae Japonicae.
abstract: Flos Lonicerae Japonicae (FLJ) is an important cash crop in eastern Asia, and it is an anti-inflammatory Traditional Chinese Medicine. There are large variations in the quality of the marketed FLJ products. To find marker ingredients useful for quality control, a tandem technology integrating ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF), principal component analysis (PCA), heat map analysis and hierarchical cluster analysis coupled with a NF-κB luciferase reporter gene assay were used to identify the different ingredients from the green bud, white bud, flowering stage and leaf stages, as well as to screen the anti-inflammatory activity of FLJ compositions. As flowering progressed, the anti-inflammatory effects of FLJ gradually decreased; however, chlorogenic acid, swertiamarin and sweroside should be used to evaluate the quality of FLJ products.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, People's Republic of China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, People's Republic of China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, People's Republic of China.
authors.forename: Yan-qi
authors.initials: YQ
authors.lastname: Han
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, People's Republic of China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, People's Republic of China.
authors.forename: Meng-ge
authors.initials: MG
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, People's Republic of China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, People's Republic of China.
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, People's Republic of China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, People's Republic of China.
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, People's Republic of China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, People's Republic of China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, People's Republic of China; Department of Chemistry, Tsinghua University, Beijing, People's Republic of China.
authors.forename: Guo-an
authors.initials: GA
authors.lastname: Luo
mesh_terms: D000893:Anti-Inflammatory Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D035264:Flowers / Q000737:chemistry; D031239:Lonicera / Q000737:chemistry*; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts