title: 1H-NMR-based profiling of organic components in leachate from animal carcasses disposal site with time.
abstract: Leachate, generated by the decomposition of animal carcasses, presents many environmental, sanitary, and food safety hazards. However, research on the characteristics of leachate is lacking. In this study, we performed biochemical profiling of leachate from two animal species (pig and cattle) in two soil types (sandy loam and sandy soil) using (1)H-NMR-based profiling, followed by multivariate data analysis. The leachate was collected from a well-controlled artificial burial site over a 31-week period. Principal components analysis (PCA) of the NMR data showed similar patterns between species and soil types. Organic components, including organic acids and phenols, predominated, and their levels increased with time. The methylamine level in leachate from pig carcasses 18 weeks following burial was significantly higher than that from cattle carcasses; leachate from cattle carcasses in sandy soil 1 week after burial contained unique components (specifically ethanol, formate, alanine, N-methylation, and taurine), in contrast with those from sandy loam soil. This study suggests that a NMR-based profiling approach is useful to characterize the organic components in leachate from animal carcasses over time.
authors.affiliation: Western Seoul Center, Korea Basic Science Institute, 150 Bugahyeon-ro, Seodamun-gu, Seoul, 120-140, Republic of Korea.
authors.forename: Yong-Kook
authors.initials: YK
authors.lastname: Kwon
authors.forename: Hyun-Whee
authors.initials: HW
authors.lastname: Bae
authors.forename: Sun Kyoung
authors.initials: SK
authors.lastname: Shin
authors.forename: Tae-Wan
authors.initials: TW
authors.lastname: Jeon
authors.forename: Jungju
authors.initials: J
authors.lastname: Seo
authors.forename: Geum-Sook
authors.initials: GS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D002417:Cattle; D003643:Death; D008744:Methylamines / Q000032:analysis; D015999:Multivariate Analysis; D010636:Phenols / Q000032:analysis; D025341:Principal Component Analysis; D066244:Proton Magnetic Resonance Spectroscopy; D012987:Soil / Q000737:chemistry*; D013045:Species Specificity; D013552:Swine; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D008744:Methylamines; D010636:Phenols; D012987:Soil; D014874:Water Pollutants, Chemical; C027451:methylamine