title: Early articular cartilage degeneration in a developmental dislocation of the hip model results from activation of β-catenin.
abstract: Developmental dislocation or dysplasia of the hip (DDH) is one of the most common deformities in children. Osteoarthritis (OA) is the most frequent long-term complication. The molecular mechanism of early articular cartilage degeneration in DDH is still unclear. It is well known that β-catenin plays a crucial role in articular cartilage degeneration. The objective of this study was to verify the relationship between β-catenin and DDH cartilage degeneration. We used a DDH model that was established by modification of swaddling position in newborn Wistar rats. The hips were isolated from the DDH model rats and untreated control group at the age of 2, 4, 6 and 8 weeks. β-Catenin gene and protein were investigated by quantitative (q)RT-PCR and immunohistochemistry. Collagen X and matrix metalloproteinase (MMP)-13, markers of early cartilage degeneration, were assessed by qRT-PCR. Primary chondrocytes were cultured from cartilage of two groups at the age of 8 weeks. Expression of β-catenin, collagen X and MMP-13 was detected. Continued high expression of β-catenin was observed in cartilage from DDH model rats. mRNA and protein expression of β-catenin was significantly increased in primary chondrocytes of the DDH model compared with the control group. Collagen X and MMP-13 expression was higher in the cartilage and chondrocytes from DDH model rats than the control group. Our findings suggest that early cartilage degeneration in DDH may result from activation of β-catenin signaling.
authors.affiliation: Department of Pediatric Orthopaedic, Anhui Provincial Children's Hospital 39 Wangjiang Road, 230051, Hefei, China ; Department of Pediatric Orthopaedic, Children's Hospital of Fudan University 399 Wanyuan Road, 201102, Shanghai, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ning
authors.affiliation: Department of Pediatric Orthopaedic, Anhui Provincial Children's Hospital 39 Wangjiang Road, 230051, Hefei, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Pediatric Orthopaedic, Anhui Provincial Children's Hospital 39 Wangjiang Road, 230051, Hefei, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Pediatric Orthopaedic, Anhui Provincial Children's Hospital 39 Wangjiang Road, 230051, Hefei, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Pediatric Orthopaedic, Children's Hospital of Fudan University 399 Wanyuan Road, 201102, Shanghai, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Pediatric Orthopaedic, Children's Hospital of Fudan University 399 Wanyuan Road, 201102, Shanghai, China.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Ma
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D002357:Cartilage Diseases / Q000378:metabolism / Q000503:physiopathology*; D002358:Cartilage, Articular / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology; D024121:Collagen Type X / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006618:Hip Dislocation, Congenital / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D024121:Collagen Type X; D051176:beta Catenin; D053509:Matrix Metalloproteinase 13
keywords: cartilage degeneration; hip dysplasia; osteoarthritis; β-catenin