title: Multi-mycotoxin analysis in dairy products by liquid chromatography coupled to quadrupole orbitrap mass spectrometry.
abstract: A new analytical method was developed and validated for simultaneous analysis of 58 mycotoxins in dairy products. Response surface methodology was employed to optimize a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation method. Ultrahigh-performance liquid chromatography and electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC/ESI Q-Orbitrap) was used for the separation and detection of all the analytes. The method was validated by taking into consideration the guidelines specified in Commission Decision 2002/657/EC and 401/2006/EC. The extraction recoveries were in a range of 86.6-113.7%, with coefficient of variation <6.2%. The 58 compounds could be detected in the 0.001-100 μg kg(-1) concentration range, with correlation coefficient >0.99. The limits of detection for the analytes are in the range 0.001-0.92 μg kg(-1). The repeatability was lower than 6.4%. This method has been successfully applied on screening of mycotoxins in commercial dairy product samples, and it is very useful for fast screening of different food contaminants.
authors.affiliation: College of Chemistry & Chemical Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
authors.affiliation: College of Chemistry & Chemical Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China. Electronic address: foodjiawei@aliyun.com.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Chu
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
authors.affiliation: College of Chemistry & Chemical Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China.
authors.forename: Junrong
authors.initials: J
authors.lastname: Huang
authors.affiliation: ThermoFisher Scientific, 355 River Oaks Parkway, San Jose, CA 95134,USA.
authors.forename: James
authors.initials: J
authors.lastname: Chang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003611:Dairy Products / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D009183:Mycotoxins / Q000032:analysis*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D009183:Mycotoxins
keywords: Dairy products; Mycotoxins; Q-Orbitrap; QuEChERS