title: Montanoa frutescens and Montanoa grandiflora extracts reduce anxiety-like behavior during the metestrus-diestrus phase of the ovarian cycle in Wistar rats.
abstract: In previous studies, the anxiolytic-like effects of Montanoa tomentosa and Montanoa frutescens were reported in male rats, but the potential anxiolytic-like effects of Montanoa plants during the different phases of the ovarian cycle in rats remain to be explored. The anxiolytic-like effects of the aqueous crude extracts of M. frutescens (25 and 50 mg/kg) and M. grandiflora (25 and 50 mg/kg) in the elevated plus maze were investigated in Wistar rats during the estrous cycle and compared with 2 mg/kg diazepam as a reference anxiolytic drug. To investigate any motor effect (i.e., hyperactivity, no changes, or hypoactivity) associated with the treatments, the rats were evaluated in the open field test. The M. frutescens (25 and 50 mg/kg) and M. grandiflora (50 mg/kg) extracts exerted anxiolytic-like effects during the metestrus-diestrus phase, similar to diazepam, without disrupting spontaneous motor activity. No significant effects of the extracts were detected in either behavioral test during the proestrus-estrus phase, whereas diazepam produced motor hypoactivity in the open field test. These results indicate that the M. frutescens and M. grandiflora extracts possess anxiolytic-like effects that depend on the ovarian cycle phase, supporting the Mexican ancient medicinal use of these plants to ameliorate anxiety disorders.
authors.affiliation: Instituto de Neuroetología, Universidad Veracruzana, Avenida Dr. Luis Castelazo s/n, Colonia Industrial Ánimas, 91001 Xalapa, VER, Mexico ; Facultad de Química Farmacéutica Biológica, Universidad Veracruzana, 91190 Xalapa, VER, Mexico.
authors.forename: Juan Francisco
authors.initials: JF
authors.lastname: Rodríguez-Landa
authors.affiliation: Facultad de Química Farmacéutica Biológica, Universidad Veracruzana, 91190 Xalapa, VER, Mexico ; Programa de Tutoría para la Investigación, Universidad Veracruzana, 91001 Xalapa, VER, Mexico.
authors.forename: Julio
authors.initials: J
authors.lastname: Vicente-Serna
authors.affiliation: Facultad de Química Farmacéutica Biológica, Universidad Veracruzana, 91190 Xalapa, VER, Mexico ; Programa de Tutoría para la Investigación, Universidad Veracruzana, 91001 Xalapa, VER, Mexico.
authors.forename: Luis Alfredo
authors.initials: LA
authors.lastname: Rodríguez-Blanco
authors.affiliation: Instituto de Neuroetología, Universidad Veracruzana, Avenida Dr. Luis Castelazo s/n, Colonia Industrial Ánimas, 91001 Xalapa, VER, Mexico.
authors.forename: María de Jesús
authors.initials: Mde J
authors.lastname: Rovirosa-Hernández
authors.affiliation: Instituto de Neuroetología, Universidad Veracruzana, Avenida Dr. Luis Castelazo s/n, Colonia Industrial Ánimas, 91001 Xalapa, VER, Mexico.
authors.forename: Francisco
authors.initials: F
authors.lastname: García-Orduña
authors.affiliation: Laboratorio de Comportamiento Reproductivo, Escuela de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tlaxcala, 90000 Tlaxcala, TLAX, Mexico.
authors.forename: Miguel
authors.initials: M
authors.lastname: Carro-Juárez
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001007:Anxiety / Q000188:drug therapy*; D001522:Behavior, Animal / Q000187:drug effects; D004031:Diestrus / Q000502:physiology*; D005260:Female; D018782:Maze Learning / Q000187:drug effects; D008686:Metestrus / Q000502:physiology*; D035924:Montanoa / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
chemical_list: D014151:Anti-Anxiety Agents; D010936:Plant Extracts