title: Characterization of RotaTeq® vaccine-derived rotaviruses in South Korean infants with rotavirus gastroenteritis.
abstract: Genotyping of human rotaviruses was performed in 191 rotavirus-positive fecal samples collected from infants with acute gastroenteritis, 3 years after the introduction of two rotavirus vaccines in South Korea. Among these samples, the most prevalent rotavirus genotype was G3P[8] (30.9%), followed by G1P[8] (27.7%), G4P[6] (15.2%), and G9P[8] (5.8%). Sequence analysis identified RotaTeq® vaccine-derived strains in 12 samples (6.3%), comprising 11 G1P[8] human-bovine double reassortant rotaviruses and 1 G1P[5] human-bovine single reassortant rotavirus. It is of note that cross-reactivity between the current G4-specific typing primer and RotaTeq®-specific G1 genotypes was found. A trace of the clinical and environmental routes of the rotavirus vaccine strains revealed unexpected complexity, and the diagnostic protocol for rotaviruses may require modification by using either another typing primer set or nucleotide sequence analysis.
authors.affiliation: Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, South Korea.
authors.forename: Van Thai
authors.initials: VT
authors.lastname: Than
authors.forename: Sunyoung
authors.initials: S
authors.lastname: Jeong
authors.forename: Wonyong
authors.initials: W
authors.lastname: Kim
mesh_terms: D002675:Child, Preschool; D016000:Cluster Analysis; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D056910:Republic of Korea / Q000453:epidemiology; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D022243:Rotavirus Vaccines / Q000008:administration & dosage*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014613:Vaccines, Attenuated / Q000008:administration & dosage
chemical_list: D012367:RNA, Viral; C492535:RotaTeq; D022243:Rotavirus Vaccines; D014613:Vaccines, Attenuated
keywords: genotyping; primer cross-reactivity; rotavirus vaccine