title: Lipid-like self-assembling peptide nanovesicles for drug delivery.
abstract: Amphiphilic self-assembling peptides are functional materials, which, depending on the amino acid sequence, the peptide length, and the physicochemical conditions, form a variety of nanostructures including nanovesicles, nanotubes, and nanovalves. We designed lipid-like peptides with an aspartic acid or lysine hydrophilic head and a hydrophobic tail composed of six alanines (i.e., ac-A6K-CONH2, KA6-CONH2, ac-A6D-COOH, and DA6-COOH). The resulting novel peptides have a length similar to biological lipids and form nanovesicles at physiological conditions. AFM microscopy and light scattering analyses of the positively charged lipid-like ac-A6K-CONH2, KA6-CONH2 peptide formulations showed individual nanovesicles. The negatively charged ac-A6D-COOH and DA6-COOH peptides self-assembled into nanovesicles that formed clusters that upon drying were organized into necklace-like formations of nanovesicles. Encapsulation of probe molecules and release studies through the peptide bilayer suggest that peptide nanovesicles may be good candidates for sustained release of pharmaceutically active hydrophilic and hydrophobic compounds. Lipid-like peptide nanovesicles represent a paradigm shifting system that may complement liposomes for the delivery of diagnostic and therapeutic agents.
authors.affiliation: School of Pharmacy, Aristotle University of Thessaloniki , GR-54124 Thessaloniki, Greece.
authors.forename: Dimitrios G
authors.initials: DG
authors.lastname: Fatouros
authors.forename: Dimitrios A
authors.initials: DA
authors.lastname: Lamprou
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Urquhart
authors.forename: Spyros N
authors.initials: SN
authors.lastname: Yannopoulos
authors.forename: Ioannis S
authors.initials: IS
authors.lastname: Vizirianakis
authors.forename: Shuguang
authors.initials: S
authors.lastname: Zhang
authors.forename: Sotirios
authors.initials: S
authors.lastname: Koutsopoulos
mesh_terms: D004337:Drug Carriers; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D008055:Lipids; D010455:Peptides