title: Basophil response to peanut allergens in Mediterranean peanut-allergic patients.
abstract: Ara h 1, Ara h 2, and Ara h 3 are important sensitizers in peanut allergy. Ara h 9 has also been shown to be relevant in the Mediterranean area. We evaluated the basophil response to peanut allergens and Pru p 3 in Mediterranean patients: Group 1, peanut and peach allergy; Group 2, peanut allergy and tolerance to peach; Group 3, peach allergy and tolerance to peanut; Group 4, nonallergic subjects that tolerate both peanut and peach. Compared to controls (Group 4), there was an increased basophil activation with Ara h 2 (P = 0.031) and Pru p 3 (P = 0.009) in Group 1 and with Ara h 1 (P = 0.016), Ara h 2 (P = 0.001), and Ara h 9 (P = 0.016) in Group 2. Importantly, only Ara h 2 showed an increased activation (P = 0.009) in Group 2 compared to Group 3. Ara h 2 is the best discriminating allergen for peanut allergy diagnosis in a Mediterranean population showing two patterns: patients also allergic to peach, responding to Ara h 2 and Pru p 3, and patients allergic only to peanut, responding to Ara h 1, Ara h 2, and Ara h 9.
authors.affiliation: Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain; UGC Allergy, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Mayorga
authors.forename: F
authors.initials: F
authors.lastname: Gomez
authors.forename: A
authors.initials: A
authors.lastname: Aranda
authors.forename: S J
authors.initials: SJ
authors.lastname: Koppelman
authors.forename: A
authors.initials: A
authors.lastname: Diaz-Perales
authors.forename: N
authors.initials: N
authors.lastname: Blanca-López
authors.forename: A B
authors.initials: AB
authors.lastname: Blazquez
authors.forename: M
authors.initials: M
authors.lastname: Blanca
authors.forename: M J
authors.initials: MJ
authors.lastname: Torres
mesh_terms: D055516:2S Albumins, Plant / Q000276:immunology*; D000328:Adult; D000485:Allergens / Q000276:immunology; D052179:Antigens, Plant / Q000276:immunology*; D015549:Basophil Degranulation Test; D001491:Basophils / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D008297:Male; D021183:Peanut Hypersensitivity / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D027861:Prunus / Q000276:immunology; D055815:Young Adult
chemical_list: D055516:2S Albumins, Plant; D000485:Allergens; D052179:Antigens, Plant; C500890:Ara h 2 allergen, Arachis hypogaea; C544631:Ara h 9 allergen, peanut; D006023:Glycoproteins; D010940:Plant Proteins; C463181:Pru p 3 allergen
keywords: allergens; basophil; lipid-transfer protein; peach; peanut