title: A dextran-bonded stationary phase for saccharide separation.
abstract: A-saccharide-based stationary phase for hydrophilic interaction liquid chromatography (HILIC) is presented. The method uses carbonyl di-imidazole (CDI) as a cross-linker for dextran in aqueous solution. Different from the traditional immobilized saccharide stationary phase, this method was using a simple way to bond the high molecular of polysaccharide immobilized on the silica gel. The new method avoided the time-consuming process but had a very considerable loading result. With silica-based surface bonded, it will be possible to develop well-defined surface modifications that promote the hydrophilic with dextran and high mechanical strength with silica. For all tested compounds, including polar compounds and carbohydrates, this dextran-bonded stationary phase performed well in terms of separation efficiency and column stability, and the retention mechanism matched that of typical HILIC retention. Moreover, good selectivity was achieved in the separation of oligosaccharides and glycopeptides.
authors.affiliation: Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Qianying
authors.initials: Q
authors.lastname: Sheng
authors.affiliation: The Second Affiliated Hospitals of Dalian Medical University, Dalian 116023, China.
authors.forename: Xindai
authors.initials: X
authors.lastname: Su
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
authors.forename: Xiuling
authors.initials: X
authors.lastname: Li
authors.affiliation: Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address: key@ecust.edu.cn.
authors.forename: Yanxiong
authors.initials: Y
authors.lastname: Ke
authors.affiliation: Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China. Electronic address: liangxm@dicp.ac.cn.
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D002241:Carbohydrates / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D003911:Dextrans / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000737:chemistry; D015203:Reproducibility of Results; D013499:Surface Properties
chemical_list: D002241:Carbohydrates; D003911:Dextrans; D006020:Glycopeptides; D007074:Immunoglobulin G
keywords: Dextran; Glycopeptides; HILIC; MS; Oligosaccharide