title: Overexpression of OsMYB4P, an R2R3-type MYB transcriptional activator, increases phosphate acquisition in rice.
abstract: R2R3 MYB transcription factors play regulatory roles in plant responses to various environmental stresses and nutrient deficiency. In this study, we isolated and designated OsMYB4P, an R2R3 MYB transcription factor, from rice (Oryza sativa L. 'Dongjin') under phosphate-deficient conditions. OsMYB4P was localized in the nucleus and acted as a transcriptional activator. Transcriptional levels of OsMYB4P in cell suspension, shoots, and roots of rice increased under phosphate-deficient conditions. Shoots and roots of OsMYB4P-overexpressing plants grew well in high- and phosphate-deficient conditions. In addition, root system architecture was altered considerably as a result of OsMYB4P overexpression. Under both phosphate-sufficient and -deficient conditions, more Pi accumulated in shoots and roots of OsMYB4P-overexpressing plants than in the wild type. Overexpression of OsMYB4P led to greater expression of Pi transporter-family proteins OsPT1, OsPT2, OsPT4, OsPT7, and OsPT8 in shoots, and to decreased or unchanged expression of these proteins in roots, with the exception of OsPT8. These results demonstrate that OsMYB4P may be associated with efficient utilization of Pi in rice through transcriptional activation of Pi homeostasis-related genes.
authors.affiliation: College of Life Science and Natural Resources, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: Won Tae
authors.initials: WT
authors.lastname: Yang
authors.affiliation: Division of Applied Life Science (BK21 Plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Dongwon
authors.initials: D
authors.lastname: Baek
authors.affiliation: Division of Applied Life Science (BK21 Plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
authors.affiliation: National Institute of Crop Science (NICS), Rural Development Administration (RDA), Suwon 441-857, Republic of Korea.
authors.forename: Woon Ha
authors.initials: WH
authors.lastname: Hwang
authors.affiliation: National Institute of Crop Science (NICS), Rural Development Administration (RDA), Suwon 441-857, Republic of Korea.
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Park
authors.affiliation: National Institute of Crop Science (NICS), Rural Development Administration (RDA), Suwon 441-857, Republic of Korea.
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Nam
authors.affiliation: College of Life Science and Natural Resources, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Chung
authors.affiliation: College of Life Science and Natural Resources, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Chung
authors.affiliation: College of Life Science and Natural Resources, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: Young Byung
authors.initials: YB
authors.lastname: Yi
authors.affiliation: College of Life Science and Natural Resources, Dong-A University, Busan 604-714, Republic of Korea. Electronic address: dhkim@dau.ac.kr.
authors.forename: Doh Hoon
authors.initials: DH
authors.lastname: Kim
mesh_terms: D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D010710:Phosphates; D010940:Plant Proteins; D014157:Transcription Factors
keywords: Phosphate acquisition; Phosphate transporter; Pi accumulation; Pi response; R2R3 MYB transcription factor; Root system architecture