title: Allergen component specific ige measurement with the Immulite™ 2000 system: diagnostic accuracy and intermethod comparison.
abstract: BACKGROUND
  The identification of the allergenic molecules, associated to the advances in the field of recombinant allergens, led to the development of a new concept in allergy diagnosis called component-resolved diagnosis. The aim of our study was to evaluate the diagnostic accuracy of different allergen components using the full automatic singleplex quantitative platform Immulite™ 2000.
  
  
  METHODS
  One hundred ninety-five allergic outpatients (35 to olive pollen, 35 to birch pollen, 35 to profilin, 35 to house dust mites, 35 to peach, and 20 to shrimp) and 20 negative controls were enrolled for the study. Bet v 1, Bet v 2, Ole e 1, Der p 1, Der p 2, Der f 1, Der f 2, Pru p 3, tropomyosin were tested both with Immulite™ 2000 and ImmunoCAP™ (Thermo Fisher Scientific, Uppsala, Sweden).
  
  
  RESULTS
  Sensitivity of allergen-specific Immunoglobulin E (sIgE) to Ole e 1, Bet v 1, Der p 1, Der p 2, Der f 1, Der f 2, Pen m 1, and Pru p 3 with Immulite™ 2000 was 100%, 100%, 77.1%, 94.3%, 71.4%, 94%, 75%, and 97.1%, respectively, and the specificity was 100% for all the allergens. The overall agreement between Immulite™ 2000 and ImmunoCAP™ (Thermo Fisher Scientific) platforms was 98.6% (Cohen's kappa = 0.979; confidence interval [CI] 95%: 0.960-0.997). From moderate to strong, positive linear correlations between the assays (r(2) from 0.322 to 0.860, and Spearman's rho from 0.824 to 0.971) were showed.
  
  
  CONCLUSIONS
  A high diagnostic accuracy of the sIgE to allergen components measurement with Immulite™ 2000 and a high agreement with ImmunoCAP™ platforms were shown in this study.
authors.affiliation: Allergologia e Immunologia Clinica, DML, A.O. "S. Maria degli Angeli", Pordenone, Italy.
authors.forename: Danilo
authors.initials: D
authors.lastname: Villalta
authors.forename: Mirella
authors.initials: M
authors.lastname: Da Re
authors.forename: Mariaelisabetta
authors.initials: M
authors.lastname: Conte
authors.forename: Paola
authors.initials: P
authors.lastname: Martelli
authors.forename: Carina Gabriela
authors.initials: CG
authors.lastname: Uasuf
authors.forename: Maria
authors.initials: M
authors.lastname: Barrale
authors.forename: Stella Maria
authors.initials: SM
authors.lastname: La Chiusa
authors.forename: Ignazio
authors.initials: I
authors.lastname: Brusca
mesh_terms: D000485:Allergens / Q000032:analysis / Q000276:immunology*; D000818:Animals; D029662:Betula / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D005512:Food Hypersensitivity / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood*; D031658:Olea / Q000276:immunology; D011058:Pollen / Q000276:immunology; D051304:Profilins / Q000276:immunology; D027861:Prunus / Q000276:immunology; D039981:Pyroglyphidae / Q000276:immunology; D012680:Sensitivity and Specificity; D012758:Shellfish; D012882:Skin Tests
chemical_list: D000485:Allergens; D051304:Profilins; D007073:Immunoglobulin E
keywords: Immulite 2000; allergy diagnosis; component resolved diagnosis; molecular allergens