title: Microarray Analysis of the Juvenile Hormone Response in Larval Integument of the Silkworm, Bombyx mori.
abstract: Juvenile hormone (JH) coordinates with 20-hydroxyecdysone (20E) to regulate larval growth and molting in insects. However, little is known about how this cooperative control is achieved during larval stages. Here, we induced silkworm superlarvae by applying the JH analogue (JHA) methoprene and used a microarray approach to survey the mRNA expression changes in response to JHA in the silkworm integument. We found that JHA application significantly increased the expression levels of most genes involved in basic metabolic processes and protein processing and decreased the expression of genes associated with oxidative phosphorylation in the integument. Several key genes involved in the pathways of insulin/insulin-like growth factor signaling (IIS) and 20E signaling were also upregulated after JHA application. Taken together, we suggest that JH may mediate the nutrient-dependent IIS pathway by regulating various metabolic pathways and further modulate 20E signaling.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, No. 2, Tiansheng Road, Beibei District, Chongqing 400715, China.
authors.forename: Daojun
authors.initials: D
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, No. 2, Tiansheng Road, Beibei District, Chongqing 400715, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, No. 2, Tiansheng Road, Beibei District, Chongqing 400715, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Meng
authors.affiliation: School of Life Science, Southwest University, Chongqing 400715, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, No. 2, Tiansheng Road, Beibei District, Chongqing 400715, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, No. 2, Tiansheng Road, Beibei District, Chongqing 400715, China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, No. 2, Tiansheng Road, Beibei District, Chongqing 400715, China.
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Xia