title: Mesenchymal stromal cells from the human placenta promote neovascularization in a mouse model in vivo.
abstract: Cell transplantation is a promising strategy in regenerative medicine for revascularization of ischemic tissues. Based on our observation that placental mesenchymal stromal cells (PMSC) enhance endothelial cell viability in vitro via secretion of angiogenic factors, we asked whether PMSC support vascular growth in vivo. PMSC were isolated from amnion and placental endothelial cells (PLEC) from chorion and either separately or co-transplanted subcutaneously into immune-deficient mice. Co-transplantation resulted in a higher number of perfused human vessels (CD31+/vimentin+) containing mouse glycophorin A+ erythrocytes. Results indicate positive effects of PMSC on neovascularization in vivo, making them attractive candidates to create autologous PMSC/PLEC pairs for research and transplantation.
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Kinzer
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Hingerl
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, Austria; School of Health and Medical Sciences, Örebro University, Örebro, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: König
authors.affiliation: Stem Cell Research Unit, Division of Hematology, Department of Internal Medicine, Medical University of Graz, Graz, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Reinisch
authors.affiliation: Experimental & Clinical Cell Therapy Institute, Spinal Cord & Tissue Regeneration Center, Paracelsus Medical University, Salzburg, Austria.
authors.forename: D
authors.initials: D
authors.lastname: Strunk
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Huppertz
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, Austria. Electronic address: ingrid.lang@medunigraz.at.
authors.forename: I
authors.initials: I
authors.lastname: Lang
mesh_terms: D000818:Animals; D042783:Endothelial Cells / Q000502:physiology / Q000637:transplantation; D005260:Female; D064593:Heterografts; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D023421:Models, Animal; D018919:Neovascularization, Physiologic; D010920:Placenta / Q000098:blood supply / Q000166:cytology*; D011247:Pregnancy; D044968:Regenerative Medicine
keywords: Angiogenesis; Human placenta; Mesenchymal stromal cells; Mouse model; Vascularization