title: Peroxisome-localized hepatitis Bx protein increases the invasion property of hepatocellular carcinoma cells.
abstract: HBx acts as a multifunctional regulator that modulates various cellular responses, which can lead to development and progression of hepatocellular carcinoma (HCC). Here, we show that the HBx protein is also localized to peroxisomes, and this increases cellular reactive oxygen species (ROS) to levels that are higher than when HBx is localized to other organelles. The elevated ROS strongly activated nuclear factor (NF)-κB. In addition, the peroxisome-localized HBx increased the expressions of matrix metalloproteinases and decreased the expression of E-cadherin, which increased the invasive ability of HCC cells. Thus, a specific distribution of HBx to peroxisomes may contribute to HCC progression by increasing the invasive ability of HCC cells through elevation of the cellular ROS level.
authors.affiliation: School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Han
authors.forename: Jung-Ah
authors.initials: JA
authors.lastname: Kang
authors.forename: Min-Hee
authors.initials: MH
authors.lastname: Han
authors.forename: Kyung-Hun
authors.initials: KH
authors.lastname: Chung
authors.forename: Cho-Rong
authors.initials: CR
authors.lastname: Lee
authors.forename: Woo-Keun
authors.initials: WK
authors.lastname: Song
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Jun
authors.forename: Sung-Gyoo
authors.initials: SG
authors.lastname: Park
mesh_terms: D015820:Cadherins / Q000096:biosynthesis; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D018450:Disease Progression; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006509:Hepatitis B / Q000821:virology; D006515:Hepatitis B virus / Q000472:pathogenicity; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics; D020783:Matrix Metalloproteinase 7 / Q000096:biosynthesis / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000473:pathology; D016328:NF-kappa B / Q000096:biosynthesis; D009361:Neoplasm Invasiveness; D020675:Peroxisomes / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D015820:Cadherins; D016328:NF-kappa B; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1