title: Applying antibiotic selection markers for nematode genetics.
abstract: Antibiotic selection markers have been recently developed in the multicellular model organism Caenorhabditis elegans and other related nematode species, opening great opportunities in the field of nematode transgenesis. Here we describe how these antibiotic selection systems can be easily combined with many well-established genetic approaches to study gene function, improving time- and cost-effectiveness of the nematode genetic toolbox.
authors.affiliation: Cancer and Human Molecular Genetics, Bellvitge Biomedical Research Institute-IDIBELL, Hospitalet de Llobregat, Barcelona 08908, Spain; Univ. Bordeaux, IECB, Laboratoire ARNA, F-33600 Pessac, France; INSERM, U869, Laboratoire ARNA, F-33000 Bordeaux, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Cornes
authors.affiliation: Univ. Bordeaux, IECB, Laboratoire ARNA, F-33600 Pessac, France; INSERM, U869, Laboratoire ARNA, F-33000 Bordeaux, France.
authors.forename: Cécile A L
authors.initials: CA
authors.lastname: Quéré
authors.affiliation: Molecular and Cellular Neurobiology Laboratory, The University of Queensland, Queensland Brain Institute, Qld 4072, Australia.
authors.forename: Rosina
authors.initials: R
authors.lastname: Giordano-Santini
authors.affiliation: Univ. Bordeaux, IECB, Laboratoire ARNA, F-33600 Pessac, France; INSERM, U869, Laboratoire ARNA, F-33000 Bordeaux, France. Electronic address: d.dupuy@iecb.u-bordeaux.fr.
authors.forename: Denis
authors.initials: D
authors.lastname: Dupuy
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015415:Biomarkers; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000235:genetics*; D018014:Gene Transfer Techniques; D009154:Mutation; D012641:Selection, Genetic; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D015415:Biomarkers
keywords: Antibiotic; C. elegans; Transformation marker; Transgenesis