title: Biological evaluation of secondary metabolites from the roots of Myrica adenophora.
abstract: Bioassay-guided fractionation of the roots of Myrica adenophora led to isolation of 24 known compounds and hitherto unknown compounds, including three A-type proanthocyanidins [adenodimerins A-C], two esters of sucrose [myricadenins A and B ], and the phenolic glycoside 6'-O-galloyl orbicularin. Spectroscopic analyses were used to determine their structures. Adenodimerin A, myricananin C, and myricetin showed strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities, with SC50 values of 7.9, 16.3, and 15.9 μM, respectively. Adenodimerin A, myricanone, myricananin C, (-)-myricanol, myricanol 11-O-β-D-glucopyranoside, and myricetin showed stronger 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activities than the positive control, with SC50 values of 7.5, 19.6, 12.0, 22.3, 19.6, and 15.6 μM, respectively. 5-Deoxymyricanone, porson, 12-hydroxymyricanone (-)-myricanol, and (+)-galeon exhibited anti-tubercular activity against Mycobacterium tuberculosis H37Rv in vitro and MICs values of 25.8, 40.0, 35.8, 30.0, and 15.0 μg/mL, respectively. Myricadenin A, myricanone, myricananin C, and (-)-myricanol exhibited anti-inflammatory activities in the iNOS assay with EC50 values of 18.1, 1.00, 13.0, and 7.5 μM, respectively.
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ROC.
authors.forename: Ya-Chih
authors.initials: YC
authors.lastname: Ting
authors.affiliation: Department of Fragrance and Cosmetics Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ROC.
authors.forename: Horng-Huey
authors.initials: HH
authors.lastname: Ko
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ROC.
authors.forename: Hui-Chun
authors.initials: HC
authors.lastname: Wang
authors.affiliation: Biomedical Laboratory Science and Biotechnology, College of Health Science, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ROC.
authors.forename: Chien-Fang
authors.initials: CF
authors.lastname: Peng
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ROC.
authors.forename: Hsun-Shuo
authors.initials: HS
authors.lastname: Chang
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ROC.
authors.forename: Pei-Chen
authors.initials: PC
authors.lastname: Hsieh
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ROC. Electronic address: m635013@kmu.edu.tw.
authors.forename: Ih-Sheng
authors.initials: IS
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D036381:Diarylheptanoids / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry; D015394:Molecular Structure; D031641:Myrica / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D036381:Diarylheptanoids; D005419:Flavonoids; D016166:Free Radical Scavengers; D006027:Glycosides; D010851:Picrates; D044945:Proanthocyanidins; C013221:proanthocyanidin; C041795:myricanone; C041794:myricanol; C040015:myricetin; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Anti-inflammatory activity; Anti-oxidant activity; Anti-tubercular activity; Diarylheptanoid; Myrica adenophora; Myricaceae; Proanthocyanidin; Root