title: [Construction and identification of pcDNA3-HBsAg-p30-ROP2 expression vector].
abstract: OBJECTIVE
  To construct a multi-gene recombinant pcDNA3-HBsAg-p30-ROP2 expression vector and identify it preliminarily.
  
  
  METHODS
  According to recombinant pcDNA3-p30-ROP2 restriction sites, HBV HBsAg gene sequences of primers were designed and synthesized to amplify target fragment, and then cloned into pcDNA3-HbsAg-p30-ROP2 expression vector. After sequencing, it was identified finally by restriction enzyme digestion and other molecular biology techniques.
  
  
  RESULTS
  HBV HBsAg gene segment was amplified by PCR and the multi-gene recombinant pcDNA3-HBsAg-p30-ROP2 expression vector was constructed and identified to be correct as theoretical values. The PCR and restriction enzyme digestion results showed that HBsAg and p30-ROP2 gene in recombinant plasmid were confirmed by DNA sequencing.
  
  
  CONCLUSIONS
  The multi-gene recombinant pcD-NA3-HBsAg-p30-ROP2 expression vector is successfully constructed.
authors.forename: Qing-kuan
authors.initials: QK
authors.lastname: Wei
authors.forename: Ying-Ting
authors.initials: YT
authors.lastname: Wang
authors.forename: Yun-Qin
authors.initials: YQ
authors.lastname: Yan
authors.forename: Ting
authors.initials: T
authors.lastname: Xiao
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
authors.forename: Gong-Zhen
authors.initials: GZ
authors.lastname: Liu
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Zhong
authors.forename: Kun
authors.initials: K
authors.lastname: Yin
authors.forename: Bin
authors.initials: B
authors.lastname: Fu
authors.forename: Ge
authors.initials: G
authors.lastname: Yan
authors.forename: Ge
authors.initials: G
authors.lastname: Yan
mesh_terms: D000953:Antigens, Protozoan / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D017325:Hepatitis B Vaccines / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics*; D016052:Protozoan Vaccines / Q000235:genetics*; D014122:Toxoplasma / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000235:genetics
chemical_list: D000953:Antigens, Protozoan; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D008565:Membrane Proteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C090555:ROP 2 protein, Toxoplasma gondii; C047168:SAG1 antigen, Toxoplasma; D014614:Vaccines, Synthetic