title: Analysis of humoral immune response and cytokines in chickens vaccinated with Eimeria brunetti apical membrane antigen-1 (EbAMA1) DNA vaccine.
abstract: This study aimed to determine the changes of cytokines, specific serum IgG and several parameters in chickens vaccinated with DNA vaccine encoding Eimeria brunetti apical membrane antigen-1 (EbAMA1) antigen. Two-week-old chickens were divided into five groups (four groups for experiment) randomly. Experimental groups of chickens were immunized with DNA vaccine while control group of chickens were injected with pVAX1 plasmid alone or TE buffer solution. All immunizations were boosted 2 weeks later. The EbAMA1 specific IgG antibody responses were measured at weeks 1-6 post-second immunizations and several parameters were also identified. The result showed that the antibody titers in chickens vaccinated with DNA vaccines were significantly different from those of the control groups 1 week after the second immunization and reached the maximum values 3 weeks post-second immunization. IFN-γ concentration was increased the highest level against EbAMA1 of all chickens vaccinated with vaccines up to 56-fold, follow by the specific IgG antibody levels were increased 10-17-fold compared with those of TE solution and plasmid (pVAX1) control chickens 1-6 weeks post-second immunization. In case of the levels of IL-10 and IL-17 was increased in experimental chickens with 4-5-fold. Even though it was statistically significant, TGF-β and IL-4 levels were higher in vaccinated than unvaccinated chickens. The results suggested that DNA vaccines encoding E. brunetti apical membrane antigen-1 (EbAMA1) could increase serum specific IgG antibody and cytokines concentration and could give protection against E. brunetti infection.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China; Faculty of Animal Husbandry and Veterinary Medicine, Bacgiang Agriculture and Forestry University, Vietyen District, Bacgiang Province, Viet Nam.
authors.forename: Tran Duc
authors.initials: TD
authors.lastname: Hoan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China.
authors.forename: Doan Thi
authors.initials: DT
authors.lastname: Thao
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China.
authors.forename: Javaid Ali
authors.initials: JA
authors.lastname: Gadahi
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China.
authors.forename: Xiaokai
authors.initials: X
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China.
authors.forename: Ruofeng
authors.initials: R
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China. Electronic address: lixiangrui@njau.edu.cn.
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D003048:Coccidiosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D016207:Cytokines / Q000097:blood; D004539:Eimeria / Q000276:immunology*; D005786:Gene Expression Regulation; D007074:Immunoglobulin G / Q000097:blood; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control; D016052:Protozoan Vaccines / Q000276:immunology*; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016207:Cytokines; D007074:Immunoglobulin G; D016052:Protozoan Vaccines; D019444:Vaccines, DNA
keywords: Apical membrane antigen protein-1 gene; Chicken; DNA vaccine; Eimeria brunetti