title: Effect of 2-aminoethoxydiphenyl borate on ischemia-reperfusion injury in a rat ovary model.
abstract: OBJECTIVE
  The aim of this study is to evaluate the effects of 2-aminoethoxydiphenyl borate (2-APB) as an antioxidant and analyze biochemical and histopathologic changes in experimental ischemia-reperfusion (I/R) injury in rat ovaries.
  
  
  METHODS
  Thirty female rats were utilized to create four groups. Group 1: I/R and 2-APB (2mg/kg); Group 2: I/R and 2-APB (4mg/kg); Group 3: I/R; Group 4: sham operation. Ovarian tissue and serum malondialdehyde, nitric oxide (NO) levels; ovarian tissue and serum total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) were determined. In ovarian tissue samples histopathologic examination, immunoflourescence staining by TUNEL method was studied.
  
  
  RESULTS
  Tissue TOS, serum TOS, and OSI levels were elevated in I/R group. After treatment with 2-APB, tissue and serum TOS levels and OSI levels were markedly decreased. There was a significant difference in terms of tissue and serum NO levels between the sham group and I/R group. Elevation in tissue NO and serum NO levels were decreased after treatment with 2-APB. TUNEL-positive cell number gradually decreased with dose of 2-APB in groups 1 and 2.
  
  
  CONCLUSIONS
  Conservative treatment with 2-APB is beneficial for mitigation of I/R injury, and the ovarian protective effect of 2-APB appears to be mediated through its antiapopitotic and antioxidative effects.
authors.affiliation: Department of Obstetrics and Gynecology, Balıkesir University School of Medicine, Balikesir, Turkey. Electronic address: minetaskin1302@yahoo.com.tr.
authors.forename: Mine Islimye
authors.initials: MI
authors.lastname: Taskin
authors.affiliation: Department of Biochemistry, Balıkesir University School of Medicine, Balikesir, Turkey.
authors.forename: Adnan Adil
authors.initials: AA
authors.lastname: Hismiogullari
authors.affiliation: Department of Histology and Embryology, Erciyes University School of Medicine, Kayseri, Turkey.
authors.forename: Arzu
authors.initials: A
authors.lastname: Yay
authors.affiliation: Department of Obstetrics and Gynecology, Balıkesir University School of Medicine, Balikesir, Turkey.
authors.forename: Ertan
authors.initials: E
authors.lastname: Adali
authors.affiliation: Department of Obstetrics and Gynecology, On Sekiz Mart University School of Medicine, Canakkale, Turkey.
authors.forename: Aysenur Cakir
authors.initials: AC
authors.lastname: Gungor
authors.affiliation: Department of Histology and Embryology, Erciyes University School of Medicine, Kayseri, Turkey.
authors.forename: Gozde Ozge
authors.initials: GO
authors.lastname: Korkmaz
authors.affiliation: Department of Obstetrics and Gynecology, Balıkesir University School of Medicine, Balikesir, Turkey.
authors.forename: Umit
authors.initials: U
authors.lastname: Inceboz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D017209:Apoptosis; D001896:Boron Compounds / Q000627:therapeutic use*; D005260:Female; D009569:Nitric Oxide / Q000378:metabolism; D010049:Ovarian Diseases / Q000188:drug therapy* / Q000473:pathology; D010053:Ovary / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D054159:Torsion, Mechanical
chemical_list: D000975:Antioxidants; D001896:Boron Compounds; D009569:Nitric Oxide; C109986:2-aminoethoxydiphenyl borate
keywords: 2-Aminoethoxydiphenyl borate (2-APB); Adnexal torsion; Ischemia-reperfusion; Rat