title: JNK and AKT/GSK3β signaling pathways converge to regulate periodontal ligament cell survival involving XIAP.
abstract: Periodontal ligament cells (PDLCs) were incubated with H2O2 and the levels of XIAP protein, protein kinase B (AKT), phosphorylated forms of AKT (pAKT), c-Jun N-terminal kinase (JNK), and glycogen synthase kinase-3β (GSK3β) were determined by western immunoblotting or immunocytochemistry. After overexpression and knockdown of XIAP, the AKT, pAKT, JNK and GSK3β levels were determined in PDLCs exposed to H2O2. We demonstrated that 72 h of 250 μM H2O2 exposure resulted in an increase in apoptosis. Meanwhile, XIAP levels were decreased with 72 h of 250 μM H2O2 exposure, while there were also a decrease of JNK2, AKT, pAKT, and GSK3β levels. Such reductions induced by 72 h of 250 μM H2O2 treatment were partially recovered in PDLCs overexpressing XIAP. Interestingly, these reductions (except for pAKT) were mimicked by RNA interference of XIAP. These results suggest that, after 72 h of 250 μM H2O2 exposure, Akt, JNK, and GSK3β intracellular kinase signaling pathways converge to regulate PDLC survival involving XIAP.
authors.affiliation: Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Mouda
authors.initials: M
authors.lastname: Wei
authors.affiliation: Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Andrew
authors.initials: A
authors.lastname: Adams
authors.affiliation: Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China. Electronic address: duanyz@fmmu.edu.cn.
authors.forename: Yinzhong
authors.initials: Y
authors.lastname: Duan
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D055785:Gene Knockdown Techniques; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D048056:Mitogen-Activated Protein Kinase 9 / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D006861:Hydrogen Peroxide; D048056:Mitogen-Activated Protein Kinase 9; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3
keywords: Apoptosis; Hydrogen peroxide; Periodontal ligament cells