title: Efficient generation human induced pluripotent stem cells from human somatic cells with Sendai-virus.
abstract: A few years ago, the establishment of human induced pluripotent stem cells (iPSCs) ushered in a new era in biomedicine. Potential uses of human iPSCs include modeling pathogenesis of human genetic diseases, autologous cell therapy after gene correction, and personalized drug screening by providing a source of patient-specific and symptom relevant cells. However, there are several hurdles to overcome, such as eliminating the remaining reprogramming factor transgene expression after human iPSCs production. More importantly, residual transgene expression in undifferentiated human iPSCs could hamper proper differentiations and misguide the interpretation of disease-relevant in vitro phenotypes. With this reason, integration-free and/or transgene-free human iPSCs have been developed using several methods, such as adenovirus, the piggyBac system, minicircle vector, episomal vectors, direct protein delivery and synthesized mRNA. However, efficiency of reprogramming using integration-free methods is quite low in most cases. Here, we present a method to isolate human iPSCs by using Sendai-virus (RNA virus) based reprogramming system. This reprogramming method shows consistent results and high efficiency in cost-effective manner.
authors.affiliation: Institute for Cell Engineering, Department of Neurology and Neuroscience, Johns Hopkins University School of Medicine.
authors.forename: In Young
authors.initials: IY
authors.lastname: Choi
authors.affiliation: Institute for Cell Engineering, Department of Neurology and Neuroscience, Johns Hopkins University School of Medicine.
authors.forename: HoTae
authors.initials: H
authors.lastname: Lim
authors.affiliation: Institute for Cell Engineering, Department of Neurology and Neuroscience, Johns Hopkins University School of Medicine; glee48@jhmi.edu.
authors.forename: Gabsang
authors.initials: G
authors.lastname: Lee
mesh_terms: D065150:Cellular Reprogramming / Q000502:physiology*; D003584:Cytological Techniques / Q000379:methods*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000821:virology*; D029082:Sendai virus / Q000502:physiology*