title: Comparison between an in-house method and the ViroSeq™ method for determining mutations for drug resistance in the HIV-1 CRF01_AE subtype circulating in China.
abstract: Commercial products for determining the drug-resistance mutations of HIV-1 are expensive and usually focus on a particular HIV-1 subtype. In this study, a more cost-effective in-house method was compared side-by-side with the ViroSeq™ Genotyping System 2.0 for determining resistant mutations in China's most prevalent subtype, CRF01_AE. Plasma samples were obtained from 205 patients infected with HIV-1, and subtypes were verified using the Stanford calibrated population resistance tool. The capacity for determining positive samples was not significantly different between the in-house (93.8%) and ViroSeq™ (96.5%) methods. For the clade of subtype CRF01_AE in particular, complete concordance between the methods was observed for the protease and reverse-transcriptase regions of the HIV-1 pol gene, and concordance for overall DRRMs and HLDRMs was 99.5% and 100%, respectively. Although 51 discordant mutations were found, further analysis verified that most of mutations had minimal impact on antiviral drugs. Excellent overall concordance (97.7%) was achieved for the resistance reports between the two methods for CRF01_AE. Thus, the performance and effectiveness of determining resistance-associated mutations were nearly equivalent between the cost-effective in-house method and the ViroSeq™ system, the greatly potential application of the in-house method in China for many patients infected with HIV-1 located in resource-limited regions.
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Hanping
authors.initials: H
authors.lastname: Li
authors.affiliation: Guangxi Center for Disease Prevention and Control, Nanning, China.
authors.forename: Shujia
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Daomin
authors.initials: D
authors.lastname: Zhuang
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Zuoyi
authors.initials: Z
authors.lastname: Bao
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Siyang
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Li
authors.affiliation: Guangxi Center for Disease Prevention and Control, Nanning, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China. Electronic address: lijy@nic.bmi.ac.cn.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D002681:China / Q000453:epidemiology; D015331:Cohort Studies; D024882:Drug Resistance, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D009154:Mutation; D017422:Sequence Analysis, DNA / Q000379:methods*; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D054302:pol Gene Products, Human Immunodeficiency Virus
keywords: CRF01_AE; Drug resistance; HIV-1; In-house method; ViroSeq™ system