title: Hybrid molecularly imprinted polymers synthesized with 3-aminopropyltriethoxysilane-methacrylic acid monomer for miniaturized solid-phase extraction: A new and economical sample preparation strategy for determination of acyclovir in urine.
abstract: The miniaturized molecularly imprinted solid-phase extraction (mini-MISPE) coupled with high-performance liquid chromatography was proposed for the determination of acyclovir in urine. 1.5-mL tapered plastic centrifuge tube filled with hybrid molecularly imprinted polymers (HMIPs) was used as the cartridge of mini-MISPE, and the HMIPs synthesized with 3-aminopropyltriethoxy silane-methacrylic acid as monomer exhibited good recognition and selectivity for acyclovir. Under the optimized condition, good linear calibration was obtained in a range of 0.5-15μgmL(-1) with the correlation coefficient of 0.9994, and the recoveries at three spiked levels were 91.6-103.3% in urine with the relative standard deviation (RSD) of ≤3.5%. Excellent intra-day and inter-day repeatability were achieved with RSD of ≤2.6% and 4.0% in three different concentrations. This method combined the advantages of HMIPs and mini-MISPE, and it could become an alternative tool for analyzing the residues of acyclovir in complex urine matrices.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education & Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China. Electronic address: yanhy@hbu.edu.cn.
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Yan
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education & Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education & Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China.
authors.forename: Yehong
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Biochemistry, Baoding University, Baoding 071002, China.
authors.forename: Fengxia
authors.initials: F
authors.lastname: Qiao
authors.affiliation: Department of Chemistry and Chemical Engineering, Inha University, Incheon 402751, Republic of Korea. Electronic address: rowkho@inha.ac.kr.
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Row
mesh_terms: D000212:Acyclovir / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D052616:Solid Phase Extraction; D016482:Urinalysis / Q000379:methods*
chemical_list: D008689:Methacrylates; D011108:Polymers; D011437:Propylamines; D012821:Silanes; C008384:methacrylic acid; C477625:amino-propyl-triethoxysilane; D000212:Acyclovir
keywords: 3-Aminopropyltriethoxysilane-methacrylic acid monomer; Acyclovir; Hybrid molecularly imprinted polymers; Miniaturized molecularly imprinted solid-phase extraction; Urine