title: Characterization of a thermophilic bacteriophage of Geobacillus kaustophilus.
abstract: GBK2 is a bacteriophage, isolated from a backyard compost pile, that infects the thermophile Geobacillus kaustophilus. GBK2 has a circularly permuted genome of 39,078 bp with a G+C content of 43 %. Annotation of the genome reveals 62 putative open reading frames (ORFs), 25 of which (40.3 %) show homology to known proteins and 37 of which (59.7 %) are proteins with unknown functions. Twelve of the identified ORFs had the greatest homology to genes from the phage SPP1, a phage that infects the mesophile Bacillus subtilis. The overall genomic arrangement of GBK2 is similar to that of SPP1, with the majority of GBK2 SPP1-like genes coding for proteins involved in DNA replication and metabolism.
authors.affiliation: Department of Pharmaceutical Sciences, Campbell University, PO Box 1090, Buies Creek, NC, 27506, USA.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Marks
authors.forename: Paul T
authors.initials: PT
authors.lastname: Hamilton
mesh_terms: D001412:Bacillus subtilis / Q000821:virology; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification; D001482:Base Composition; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D056506:Geobacillus / Q000821:virology*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins