title: Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells.
abstract: Lactobacillus acidophilus is categorized as a probiotic strain because of its beneficial effects in human health and prevention of disease transmission. This study is aimed to characterize the probiotic potential of L. acidophilus 36YL originally isolated from the vagina of healthy and fertile Iranian women. The L. acidophilus 36YL strain was identified using 16S rDNA gene sequencing and characterized by biochemical methodologies, such as antibiotics susceptibility, antimicrobial activity, and acid and bile resistance. The bioactivity of the secretion of this strain on four human cancer cell lines (AGS, HeLa, MCF-7, and HT-29) and one normal cell line (HUVEC) was evaluated by cytotoxicity assay and apoptosis analysis. This newly isolated strain was found to exhibit notable probiotic properties, such as admirable antibiotic susceptibility, good antimicrobial activity, and favorable resistance to acid and bile salt. The results of bioactivity assessment demonstrated acceptable anticancer effects on the four tested cancer cell lines and negligible side effects on the assayed normal cell line. Our findings revealed that the anticancer effect of L. acidophilus 36YL strain secretions depends on the induction of apoptosis in cancer cells. L. acidophilus 36YL strain is considered as a nutraceutical alternative or a topical medication with a potential therapeutic index because of the absence of cytotoxicity to normal cells, but effective toxicity to cancer cell lines.
authors.affiliation: Institute of Biosciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Yousef
authors.initials: Y
authors.lastname: Nami
authors.affiliation: Chemical and Environmental Engineering Department, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: nhafizah@eng.upm.edu.my.
authors.forename: Norhafizah
authors.initials: N
authors.lastname: Abdullah
authors.affiliation: Institute of Biosciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Babak
authors.initials: B
authors.lastname: Haghshenas
authors.affiliation: Chemical and Environmental Engineering Department, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Dayang
authors.initials: D
authors.lastname: Radiah
authors.affiliation: Institute of Biosciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Rozita
authors.initials: R
authors.lastname: Rosli
authors.affiliation: Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: Yarikhosroushahia@tbzmed.ac.ir.
authors.forename: Ahmad Yari
authors.initials: AY
authors.lastname: Khosroushahi
mesh_terms: D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000378:metabolism; D000898:Antibiosis; D017209:Apoptosis; D015373:Bacterial Typing Techniques; D001646:Bile / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D007779:Lactobacillus acidophilus / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D019936:Probiotics / Q000302:isolation & purification* / Q000494:pharmacology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014621:Vagina / Q000382:microbiology; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D002264:Carboxylic Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Anti-microbe; Antibiotic susceptibility; Anticancer; Apoptosis; Cytotoxicity