title: Extracts and Fractions from Edible Roots of Sechium edule (Jacq.) Sw. with Antihypertensive Activity.
abstract: Sechium edule is traditionally used in Mexico as a therapeutic resource against renal diseases and to control high blood pressure. The purpose of this work is to evaluate the antihypertensive effect of the hydroalcoholic extract obtained from the roots of this plant, including its fractions and subfractions, on different hypertension models induced with angiotensin II (AG II). The hydroalcoholic extract was tested on an in vitro study of isolated aorta rings denuded of endothelial cells, using AG II as the agonist; this assay proved the vasorelaxant effect of this extract. Vagotomized rats were administered different doses of AG II as well as the Hydroalcoholic extract, which reduced blood pressure in 30 mmHg approximately; subsequently this extract was separated into two fractions (acetone and methanol) which were evaluated in the acute hypertension mouse model induced with AG II, where the acetone fraction was identified as the most effective one and was subsequently subfractioned using an open chromatographic column packed with silica gel. The subfractions were also evaluated in the acute hypertension model. Finally, the extract, fraction, and active subfraction were analyzed by MS-PDA-HPLC, identifying cinnamic derivative compounds like cinnamic acid methyl ester.
authors.affiliation: Programa de Doctorado en Ciencias Biológicas y de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico ; Centro de Investigación Biomédica del Sur (CIBIS-IMSS), Argentina 1, Colonia Centro, 62790 Xochitepec, MOR, Mexico.
authors.forename: Galia
authors.initials: G
authors.lastname: Lombardo-Earl
authors.affiliation: Programa de Doctorado en Ciencias Biológicas y de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico.
authors.forename: Rubén
authors.initials: R
authors.lastname: Roman-Ramos
authors.affiliation: Centro de Investigación Biomédica del Sur (CIBIS-IMSS), Argentina 1, Colonia Centro, 62790 Xochitepec, MOR, Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Zamilpa
authors.affiliation: Centro de Investigación Biomédica del Sur (CIBIS-IMSS), Argentina 1, Colonia Centro, 62790 Xochitepec, MOR, Mexico.
authors.forename: Maribel
authors.initials: M
authors.lastname: Herrera-Ruiz
authors.affiliation: Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Calle Iztaccihuátl Esq. Leñeros S/N, Colonia Volcanes, 62350 Cuernavaca, MOR, Mexico.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Rosas-Salgado
authors.affiliation: Centro de Investigación Biomédica del Sur (CIBIS-IMSS), Argentina 1, Colonia Centro, 62790 Xochitepec, MOR, Mexico.
authors.forename: Jaime
authors.initials: J
authors.lastname: Tortoriello
authors.affiliation: Centro de Investigación Biomédica del Sur (CIBIS-IMSS), Argentina 1, Colonia Centro, 62790 Xochitepec, MOR, Mexico.
authors.forename: Enrique
authors.initials: E
authors.lastname: Jiménez-Ferrer