title: Mapping protein complexes using covalently linked antibodies and isobaric mass tags.
abstract: Affinity enrichment techniques in combination with quantitative proteomics enable the unbiased identification of protein-protein interaction, and thus the delineation of protein complexes and interaction networks. Here, we describe an immunoaffinity enrichment approach that employs covalently immobilized antibodies for the identification of protein-protein interactions of endogenously expressed proteins under near-to-physiological conditions. Specifically enriched proteins are identified using shotgun mass spectrometry and isobaric mass tag-based relative quantification.
authors.affiliation: Cellzome GmbH, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Dittmann
authors.forename: Sonja
authors.initials: S
authors.lastname: Ghidelli-Disse
authors.forename: Carsten
authors.initials: C
authors.lastname: Hopf
authors.forename: Marcus
authors.initials: M
authors.lastname: Bantscheff
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D002853:Chromatography, Liquid; D011506:Proteins / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D000906:Antibodies; D011506:Proteins