title: Quantification of proteins by label-free LC-MS(E.).
abstract: Quantitative proteomics by LC-MS/MS is a widely used approach for quantifying a significant portion of any complex proteome. Among the different techniques used for this purpose, one is by use of Data Independent Acquisition (DIA). We present a descriptive protocol for label-free quantitation of proteins by one DIA method termed LC-MS(E), which facilitates large-scale quantification of proteins without the need for isotopic labelling and with no theoretical limit to the number of samples included in an experiment.
authors.affiliation: Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, 76100, Israel.
authors.forename: Alon
authors.initials: A
authors.lastname: Savidor
authors.forename: Yishai
authors.initials: Y
authors.lastname: Levin
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011506:Proteins