title: Expression differences of miRNAs and genes on NF-κB pathway between the healthy and the mastitis Chinese Holstein cows.
abstract: In order to discover the variation of microRNAs and genes associated with NF-κB signaling pathway between the healthy and the mastitis Chinese Holstein cows, Illumina Deep Sequencing and qRT-PCR are applied to detect 25 kinds of miRNAs (miR-16, miR-125b, miR-15, miR-29a, miR-23b, miR-146, miR-301a, miR-181b, let-7, miR-30b, miR-21, miR-223, miR-27b, miR-10a, miR-143, etc.) expression levels in blood samples and 14 genes (RelA, RelB, Rel, p105, p100, IκBα, IκBβ, IκBδ, IκBε, IκBζ, Bcl-3, IKKα, IKKβ, IKKγ/NEMO) relative expression levels in nine tissues. The total number of miRNAs is declining, and RelA, Rel, p105, p100, IκBα, IκBβ, IκBδ, IκBζ, Bcl-3, and IKKα expressions are rising in mastitis individuals. So, we suppose that NF-κB pathway is active in mastitis individuals as a result of the decrease inhibition of miRNAs. While in healthy ones, the NF-κB pathway is inactive, because of the miRNAs enhanced inhibition action. However, the specific regulatory mechanism of miRNAs on NF-κB pathway in mastitis Holstein cows needs further investigation. Moreover, due to obvious expression differences, some miRNAs, especially miR-16 and miR-223, may be used as new markers for the dairy mastitis prognosing.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China. Electronic address: liuxiaolin@nwsuaf.edu.cn.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Fubiao
authors.initials: F
authors.lastname: Niu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Jiazhong
authors.initials: J
authors.lastname: Guo
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D002417:Cattle; D005260:Female; D005786:Gene Expression Regulation; D005819:Genetic Markers; D008414:Mastitis, Bovine / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs; D016328:NF-kappa B
keywords: Expression differences; Genes of NF-κB pathway; Mastitis; miRNAs