title: Evalution of DNA extraction methods in order to monitor genetically modified materials in soy foodstuffs and feeds commercialised in Turkey by multiplex real-time PCR.
abstract: BACKGROUND
  Soybean is one of the most important biotech crops, widely used as an ingredient in both foodstuffs and feed. DNA extraction methods have been evaluated to detect the presence of genetically modified (GM) materials in soya-containing food and feed products commercialised in Turkey.
  
  
  RESULTS
  All extraction methods performed well for the majority of soya foods and feed products analysed. However, the most successful method varied between different products; the Foodproof, Genespin and the cetyltrimethylammonium bromide (CTAB) methods each produced the highest DNA yield and purity for different soya foodstuffs and feeds. Of the samples tested, 20% were positive for the presence of at least two GM elements (35S/NOS) while 11% contained an additional GM element (35S/NOS/FMV). Of the tested products, animal feeds showed a larger prevalence of GM material (50%) than the soya-containing foodstuffs (13%).
  
  
  CONCLUSIONS
  The best performing extraction methods proved to be the Foodproof, Genespin and CTAB methods for soya-containing food and feed products. The results obtained herein clearly demonstrate the presence of GM soybean in the Turkish market, and that the Foodproof GMO Screening Kit provides reliable screening of soy-containing food and feed products.
authors.affiliation: Department of Plant and Animal Production, Vocational School of Mustafakemalpasa, University of Uludag, 16500, Bursa, Turkey.
authors.forename: Aydin
authors.initials: A
authors.lastname: Turkec
authors.forename: Hande
authors.initials: H
authors.lastname: Kazan
authors.forename: Aykut
authors.initials: A
authors.lastname: Baykut
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Lucas
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D018744:DNA, Plant / Q000302:isolation & purification*; D030841:Food, Genetically Modified; D006801:Humans; D030821:Plants, Genetically Modified / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds; D045730:Soy Foods / Q000032:analysis*; D013025:Glycine max / Q000235:genetics*; D014421:Turkey
chemical_list: D018744:DNA, Plant
keywords: DNA extraction; GM soy; feed; food; multiplex PCR screening; real-time PCR