title: [Improved preparation of pure alive eggs of Schistosoma japonicum].
abstract: To prepare a large amount of pure alive Schistosoma japonicum eggs, rabbit was infected with 2000 cercariae and its liver was taken aseptically 38-45 days after infection and homogenized. The homogenate was screened through different sieves(60, 120, 200, 300, 360 meshes per inch respectively), and washed with 1.2% NaCl. The eggs and leftover were then digested with 0.25% trypsin for 2 hours, sieved over 360 meshes per inch and washed with RPMI 1640 medium. The collected eggs reached to (95.1 +/- 6.4)% of live eggs, with a high efficiency.
authors.forename: Yan-Hai
authors.initials: YH
authors.lastname: Wang
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Peng
authors.forename: Jin-Bao
authors.initials: JB
authors.lastname: Gu
mesh_terms: D000818:Animals; D005260:Female; D008099:Liver / Q000469:parasitology*; D008297:Male; D010270:Parasite Egg Count; D011817:Rabbits; D012549:Schistosoma japonicum / Q000302:isolation & purification*; D012554:Schistosomiasis japonica / Q000469:parasitology*