title: Subchronic toxicity study in rats of two new ethyl-carbamates with ixodicidal activity.
abstract: Female and male Wistar rats were used to determine the subchronic oral toxicities of two new ethyl-carbamates with ixodicidal activities (ethyl-4-bromphenyl-carbamate and ethyl-4-chlorphenyl-carbamate). The evaluated carbamates were administered in the drinking water (12.5, 25 and 50 mg/kg/day) for 90 days. Exposure to the evaluated carbamates did not cause mortality or clinical signs and did not affect food consumption or weight gain. However, exposure to these carbamates produced alterations in water consumption, hematocrit, percentages of reticulocytes, plasma proteins, some biochemical parameters (aspartate aminotransferase, gamma-glutamyl transpeptidase, cholinesterase, and creatinine activities), thiobarbituric acid reactive substances, and the relative weight of the spleen. Histologically, slight pathological alterations were found in the liver that were consistent with the observed biochemical alterations. The nonobserved adverse effect levels (NOAELs) of the evaluated carbamates were 12.5 mg/kg/day for both the female and male rats. The low severity and reversibility of the majority of the observed alterations suggest that the evaluated carbamates have low subchronic toxicity.
authors.affiliation: Programa de Maestría y Doctorado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México, 04510 Ciudad Universitaria, D.F., Mexico.
authors.forename: María Guadalupe
authors.initials: MG
authors.lastname: Prado-Ochoa
authors.affiliation: Laboratorio de Química Medicinal, Departamento de Ciencias Químicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54714 Cuautitlán Izcalli, Mexico.
authors.forename: Víctor Hugo
authors.initials: VH
authors.lastname: Abrego-Reyes
authors.affiliation: Laboratorio de Química Medicinal, Departamento de Ciencias Químicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54714 Cuautitlán Izcalli, Mexico.
authors.forename: Ana María
authors.initials: AM
authors.lastname: Velázquez-Sánchez
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54714 Cuautitlán Izcalli, Mexico.
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Muñoz-Guzmán
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54714 Cuautitlán Izcalli, Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: Ramírez-Noguera
authors.affiliation: Laboratorio de Química Medicinal, Departamento de Ciencias Químicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54714 Cuautitlán Izcalli, Mexico.
authors.forename: Enrique
authors.initials: E
authors.lastname: Angeles
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54714 Cuautitlán Izcalli, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Alba-Hurtado
mesh_terms: D056810:Acaricides / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004327:Drinking Behavior / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008970:Molecular Weight; D009929:Organ Size / Q000187:drug effects; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D036401:Toxicity Tests, Chronic; D014520:Urethane / Q000737:chemistry / Q000633:toxicity*
chemical_list: D056810:Acaricides; D017392:Thiobarbituric Acid Reactive Substances; D014520:Urethane