title: Characterization of family D DNA polymerase from Thermococcus sp. 9°N.
abstract: Accurate DNA replication is essential for maintenance of every genome. All archaeal genomes except Crenarchaea, encode for a member of Family B (polB) and Family D (polD) DNA polymerases. Gene deletion studies in Thermococcus kodakaraensis and Methanococcus maripaludis show that polD is the only essential DNA polymerase in these organisms. Thus, polD may be the primary replicative DNA polymerase for both leading and lagging strand synthesis. To understand this unique archaeal enzyme, we report the biochemical characterization of a heterodimeric polD from Thermococcus. PolD contains both DNA polymerase and proofreading 3'-5' exonuclease activities to ensure efficient and accurate genome duplication. The polD incorporation fidelity was determined for the first time. Despite containing 3'-5' exonuclease proofreading activity, polD has a relatively high error rate (95 × 10(-5)) compared to polB (19 × 10(-5)) and at least 10-fold higher than the polB DNA polymerases from yeast (polε and polδ) or Escherichia coli DNA polIII holoenzyme. The implications of polD fidelity and biochemical properties in leading and lagging strand synthesis are discussed.
authors.affiliation: New England Biolabs, Inc., 240 County Road, Ipswich, MA, 01938, USA.
authors.forename: Lucia
authors.initials: L
authors.lastname: Greenough
authors.forename: Julie F
authors.initials: JF
authors.lastname: Menin
authors.forename: Nirav S
authors.initials: NS
authors.lastname: Desai
authors.forename: Zvi
authors.initials: Z
authors.lastname: Kelman
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Gardner
mesh_terms: D015111:4-Hydroxyphenylpyruvate Dioxygenase / Q000235:genetics / Q000378:metabolism*; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004261:DNA Replication; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D019712:Thermococcus / Q000201:enzymology*
chemical_list: D019843:Archaeal Proteins; D015111:4-Hydroxyphenylpyruvate Dioxygenase