title: Genetic instability in peripheral lymphocytes as biological marker for non-small cell lung cancer patients in the South Indian state of Andhra Pradesh.
abstract: OBJECTIVE
  This study aims, first, at evaluating the DNA and chromosomal damage in non-small cell lung cancer (NSCLC) patients from the South Indian state of Andhra Pradesh, and then at correlating these results with possible confounding factors that might potentially play a role in causing genetic damage.
  
  
  METHODS
  The study included 246 NSCLC patients (177 men and 69 women) and 250 healthy controls (180 men and 70 women) for the analysis of DNA and chromosomal damage using the comet assay and micronucleus test.
  
  
  RESULTS
  Both DNA and chromosomal damage were found to be increased in NSCLC patients compared to healthy controls, and the extent of the damage was higher in males than female patients. The smoking status had a profound effect on the extent of DNA and chromosomal damage in NSCLC patients. The degree of genetic damage correlated with the stage of the disease. However, the histological status had no effect on the extent of DNA and chromosomal damage among NSCLC patients.
  
  
  CONCLUSIONS
  We here report, for the first time, that the NSCLC patients selected form the Andhra Pradesh population had increased DNA damage and higher mean micronucleus frequencies in peripheral lymphocytes, indicating a strong background level of genetic instability.
authors.affiliation: 1 Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, Andhra Pradesh - India.
authors.forename: Vidyullatha
authors.initials: V
authors.lastname: Peddireddy
authors.forename: Siva Prasad
authors.initials: SP
authors.lastname: Badabagni
authors.forename: Sandhya Devi
authors.initials: SD
authors.lastname: Gundimeda
authors.forename: Shehnaz
authors.initials: S
authors.lastname: Sultana
authors.forename: Kanakadurga
authors.initials: K
authors.lastname: Kadali
authors.forename: Suresh
authors.initials: S
authors.lastname: Gudala
authors.forename: Pardhanandana Reddy
authors.initials: PR
authors.lastname: Penagaluru
authors.forename: Hema Prasad
authors.initials: HP
authors.lastname: Mundluru
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000428:Alcohol Drinking / Q000009:adverse effects; D014408:Biomarkers, Tumor / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D020552:Comet Assay; D004249:DNA Damage / Q000235:genetics*; D005260:Female; D006801:Humans; D007194:India; D008175:Lung Neoplasms / Q000235:genetics*; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D008875:Middle Aged; D012907:Smoking / Q000009:adverse effects
chemical_list: D014408:Biomarkers, Tumor