title: Enhancement of DNA vaccine potency against legumain.
abstract: The asparaginyl endopeptidase legumain that is overexpressed in M2-polarized tumor-associated macrophages has been identified as a suitable target for elimination of these cells supporting tumor progression. To enhance the efficacy of DNA immunization against legumain, we performed several modifications in this protein that could improve induction of immune responses. First, we mutated the RGD motif into GGD or RGG sequences. This alteration resulted in diminished maturation of legumain and impaired cellular localization. Then, as tolerance to self-antigens can be broken by the activation of CD4 T-cell help, we tried to enhance the immunogenicity of legumain by the insertion of a foreign helper epitope, namely the p30 epitope from the tetanus toxin. Finally, the 2 modifications were combined. After gene gun DNA immunization of C57BL/6 mice with these constructs, we identified the Lgmn111-119 CD8 T-cell epitope that binds to H-2D molecules. Furthermore, we showed that mutagenesis in the RGD motif significantly enhanced the immune response against legumain. The addition of the p30 helper epitope induced the specific production of IFN-γ by T cells, but did not significantly increase legumain-specific immunity activated after mutagenesis in the RGD motif which might be caused by simultaneous activation of a Th2 response demonstrated by the production of IL-4. However, the beneficial effect of the helper epitope on legumain-specific response was proved after the depletion of regulatory T cells by antibody against CD25 that preferentially stimulated Th1 immunity. The antitumor effect of the modified legumain gene was shown in the immunization against tumors induced by MK16 cells.
authors.affiliation: Department of Experimental Virology, Institute of Hematology and Blood Transfusion, Prague 2, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Smahel
authors.forename: Martina
authors.initials: M
authors.lastname: Duskova
authors.forename: Ingrid
authors.initials: I
authors.lastname: Polakova
authors.forename: Jan
authors.initials: J
authors.lastname: Musil
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000818:Animals; D019470:Biolistics; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D019496:Cancer Vaccines; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000378:metabolism; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007371:Interferon-gamma / Q000378:metabolism; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016297:Mutagenesis, Site-Directed; D009154:Mutation / Q000235:genetics; D041681:NIH 3T3 Cells; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy*; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D013744:Tetanus Toxin / Q000235:genetics / Q000378:metabolism; D019444:Vaccines, DNA; D000099240:Asparaginyl Endopeptidase
chemical_list: D014408:Biomarkers, Tumor; D019496:Cancer Vaccines; D003546:Cysteine Endopeptidases; D018984:Epitopes, T-Lymphocyte; D007371:Interferon-gamma; D010446:Peptide Fragments; D013744:Tetanus Toxin; D019444:Vaccines, DNA; D000099240:Asparaginyl Endopeptidase; C091828:P30 tetanus toxin peptide