title: Mitochondria hyperfusion and elevated autophagic activity are key mechanisms for cellular bioenergetic preservation in centenarians.
abstract: Mitochondria have been considered for long time as important determinants of cell aging because of their role in the production of reactive oxygen species. In this study we investigated the impact of mitochondrial metabolism and biology as determinants of successful aging in primary cultures of fibroblasts isolated from the skin of long living individuals (LLI) (about 100 years old) compared with those from young (about 27 years old) and old (about 75 years old) subjects. We observed that fibroblasts from LLI displayed significantly lower complex I-driven ATP synthesis and higher production of H2O2 in comparison with old subjects. Despite these changes, bioenergetics of these cells appeared to operate normally. This lack of functional consequences was likely due to a compensatory phenomenon at the level of mitochondria, which displayed a maintained supercomplexes organization and an increased mass. This appears to be due to a decreased mitophagy, induced by hyperfused, elongated mitochondria. The overall data indicate that longevity is characterized by a preserved bioenergetic function likely attained by a successful mitochondria remodeling that can compensate for functional defects through an increase in mass, i.e. a sort of mitochondrial "hypertrophy".
authors.affiliation: DIBINEM, Department of Biomedical and Neuromotor Sciences University of Bologna, 40126 Bologna, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Sgarbi
authors.forename: Paola
authors.initials: P
authors.lastname: Matarrese
authors.forename: Marcello
authors.initials: M
authors.lastname: Pinti
authors.forename: Catia
authors.initials: C
authors.lastname: Lanzarini
authors.forename: Barbara
authors.initials: B
authors.lastname: Ascione
authors.forename: Lara
authors.initials: L
authors.lastname: Gibellini
authors.forename: Emi
authors.initials: E
authors.lastname: Dika
authors.forename: Annalisa
authors.initials: A
authors.lastname: Patrizi
authors.forename: Chiara
authors.initials: C
authors.lastname: Tommasino
authors.forename: Miriam
authors.initials: M
authors.lastname: Capri
authors.forename: Andrea
authors.initials: A
authors.lastname: Cossarizza
authors.forename: Alessandra
authors.initials: A
authors.lastname: Baracca
authors.forename: Giorgio
authors.initials: G
authors.lastname: Lenaz
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Solaini
authors.forename: Claudio
authors.initials: C
authors.lastname: Franceschi
authors.forename: Walter
authors.initials: W
authors.lastname: Malorni
authors.forename: Stefano
authors.initials: S
authors.lastname: Salvioli
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over / Q000502:physiology*; D000375:Aging / Q000502:physiology*; D001343:Autophagy / Q000502:physiology*; D015153:Blotting, Western; D002478:Cells, Cultured; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000502:physiology*