title: Mycobacterium tuberculosis persistence in various adipose depots of infected mice and the effect of anti-tubercular therapy.
abstract: The adipocytes are one of the non-professional phagocytes postulated to be a haven for Mycobacterium tuberculosis during persistence in the human host. The adipocyte - M. tuberculosis interaction data available to date are ex vivo. The present study was primarily aimed to investigate M. tuberculosis infection of adipocytes in course of infection of mouse model. Using primary murine adipocytes, the study first confirmed the infection and immunomodulation of natural adipocytes by M. tuberculosis. The bacilli could be isolated form visceral, subcutaneous, peri renal and mesenteric adipose depots of immunocompetent mice infected with M. tuberculosis intravenously. The bacilli could be isolated from adipocytes and the stromal vascular fraction, even though the numbers were significantly higher in the latter. The bacterial burden in the adipose depots was comparable to those in lungs in the early phase of infection. But with time, the burden in the adipose depots was either decreased or kept under control, despite the increasing burden in the lungs. Infected mice treated with standard anti tubercular drugs, despite effective elimination of bacterial loads in the lungs, continued to harbour M. tuberculosis in adipose depots at loads similar to untreated mice in the late infection phase.
authors.affiliation: Division of Microbiology, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Agarwal
authors.affiliation: Division of Microbiology, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India.
authors.forename: Shaheb R
authors.initials: SR
authors.lastname: Khan
authors.affiliation: Division of Microbiology, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India.
authors.forename: Subash C
authors.initials: SC
authors.lastname: Verma
authors.affiliation: Division of Pharmacology, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India.
authors.forename: Muheeb
authors.initials: M
authors.lastname: Beg
authors.affiliation: Electron Microscopy Lab, Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India.
authors.forename: Kavita
authors.initials: K
authors.lastname: Singh
authors.affiliation: Electron Microscopy Lab, Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India; Academy of Scientific and Innovative Research, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India.
authors.forename: Kalyan
authors.initials: K
authors.lastname: Mitra
authors.affiliation: Division of Pharmacology, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India; Academy of Scientific and Innovative Research, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India.
authors.forename: Anil N
authors.initials: AN
authors.lastname: Gaikwad
authors.affiliation: Division of Molecular and Structural Biology, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India; Academy of Scientific and Innovative Research, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India.
authors.forename: Md Sohail
authors.initials: MS
authors.lastname: Akhtar
authors.affiliation: Division of Microbiology, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India; Academy of Scientific and Innovative Research, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226 031, Uttar Pradesh, India. Electronic address: manju@cdri.res.in.
authors.forename: Manju Y
authors.initials: MY
authors.lastname: Krishnan
mesh_terms: D017667:Adipocytes / Q000737:chemistry / Q000276:immunology* / Q000382:microbiology*; D054392:Adipokines / Q000032:analysis; D000273:Adipose Tissue / Q000382:microbiology; D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D054884:Host-Pathogen Interactions / Q000276:immunology; D066292:Lipid Droplets; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity; D014376:Tuberculosis / Q000188:drug therapy / Q000382:microbiology*
chemical_list: D054392:Adipokines; D000995:Antitubercular Agents; D016207:Cytokines
keywords: Adipocytes; Mouse adipose depots; Mycobacterium tuberculosis; Persistence