title: Vaccination studies: detection of a Listeria monocytogenes-specific T cell immune response using the ELISPOT technique.
abstract: During systemic infection by Listeria monocytogenes the host develops a robust T cell-mediated immune response against the major immunodominant antigens of the pathogen. The enzyme-linked immuno-spot (ELISPOT) test is an accurate and reproducible means of measuring the extent of this T cell response. Here we describe a detailed ELISPOT protocol for measuring an epitope-specific CD8+ T cell-mediated immune response in mice vaccinated with low doses of L. monocytogenes. The basic approach can be easily adapted for the analysis of other vaccination regimes and target epitopes.
authors.affiliation: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Bahey-El-Din
authors.forename: Cormac G M
authors.initials: CG
authors.lastname: Gahan
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D007124:Immunoenzyme Techniques / Q000379:methods*; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D018984:Epitopes, T-Lymphocyte