title: Supplementation of folic acid and vitamin B₁₂ reduces plasma levels of asymmetric dimethylarginine in patients with acute ischemic stroke.
abstract: Increased levels of asymmetric dimethylarginine (ADMA) have been observed in patients with acute ischemic stroke. We aimed to investigate the correlation between ADMA and ischemic stroke, and evaluate the effect of supplementation of folic acid and vitamin B12 on concentrations of ADMA. Patients were randomized into intervention and non-intervention groups within 3 days after symptom onset. Intervention group patients were treated with folic acid (5mg daily) and vitamin B12 (500 μg twice daily) for 12 weeks. ADMA and homocysteine (Hcy) concentrations were measured before treatment (baseline) and 2 and 12 weeks after treatment. The laboratory measures were also collected from healthy controls. Eighty five subjects were enrolled in this study, from whom 72 with complete baseline and follow-up laboratory data were included in the present analysis. Thirty four patients were assigned to the intervention group and 38 patients to the non-intervention group. Sixty people were enrolled as healthy controls. Levels of ADMA and Hcy were raised (p<0.05) in patients with acute ischemic stroke. With supplementation of both folic acid and vitamin B12, the levels of ADMA and Hcy decreased significantly at 2 and 12 weeks (p<0.05). The present study reconfirmed that ADMA can be regarded as a risk biomarker for acute ischemic stroke. We observed that with supplementation of folic acid and vitamin B12, levels of ADMA were decreased in patients with acute ischemic stroke.
authors.affiliation: Department of Neurology, The Second Hospital of Tianjin Medical University, No. 23, Pingjiang Road, Hexi District, Tianjin 300211, China.
authors.forename: Xiao-Shuang
authors.initials: XS
authors.lastname: Xia
authors.affiliation: Department of Neurology, The Second Hospital of Tianjin Medical University, No. 23, Pingjiang Road, Hexi District, Tianjin 300211, China. Electronic address: lixinsci@126.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Geratology, The Second Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Second Hospital of Tianjin Medical University, No. 23, Pingjiang Road, Hexi District, Tianjin 300211, China.
authors.forename: Ji-Zuo
authors.initials: JZ
authors.lastname: Wang
authors.affiliation: Department of Geratology, The Second Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Ma
authors.affiliation: Department of Geratology, The Second Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Cun-Jin
authors.initials: CJ
authors.lastname: Wu
mesh_terms: D000368:Aged; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D001774:Blood Chemical Analysis; D002545:Brain Ischemia / Q000097:blood* / Q000188:drug therapy*; D019587:Dietary Supplements; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage*; D005500:Follow-Up Studies; D006710:Homocysteine / Q000097:blood; D006801:Humans; D008297:Male; D009569:Nitric Oxide / Q000097:blood; D052250:Nitric Oxide Synthase Type III / Q000097:blood; D020521:Stroke / Q000097:blood* / Q000188:drug therapy*; D013997:Time Factors; D016896:Treatment Outcome; D014805:Vitamin B 12 / Q000008:administration & dosage*
chemical_list: D006710:Homocysteine; D009569:Nitric Oxide; C018524:N,N-dimethylarginine; D005492:Folic Acid; D001120:Arginine; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D014805:Vitamin B 12
keywords: Asymmetric dimethylarginine; Folic acid; Homocysteinemia; Ischemic stroke; Vitamin B(12)