title: Roles of carbonic anhydrase 8 in neuronal cells and zebrafish.
abstract: BACKGROUND
  Carbonic anhydrase 8 (CA8) is an isozyme of α-carbonic anhydrases (CAs). Previous studies showed that CA8 can be detected in human adult brain, with more intense expression in the cerebellum. Single mutations in CA8 were reported to cause novel syndromes like ataxia, mild mental retardation or the predisposition to quadrupedal gait.
  
  
  METHODS
  In the present study, we examine the functions of CA8 in neuronal cell lines, mouse cerebellar granule neurons and zebrafish.
  
  
  CONCLUSIONS
  We demonstrated that overexpression of CA8 in neuronal cells significantly decreased cell death under staurosporine treatment. Moreover, CA8 overexpression significantly increased cell migration and invasion ability in neuronal cells and in mouse cerebellar granule neurons, implicating that CA8 may be involved in neuron motility and oncogenesis. By using zebrafish as an animal model, motor reflection of 3dpf zebrafish embryos was significantly affected after the down-regulation of CA8 through ca8 morpholino.
  
  
  CONCLUSIONS
  We concluded that CA8 overexpression desensitizes neuronal cells to STS induced apoptotic stress and increases cell migration and invasion ability in neuronal cells. In addition, down-regulated CA8 decreases neuron mobility in neuronal cells and leads to abnormal calcium release in cerebellar granule neurons. Knockdown of the ca8 gene results in an abnormal movement pattern in zebrafish.
  
  
  CONCLUSIONS
  Our findings provide evidence to support that the impaired protective function of CA8 contributes to human neuropathology, and to suggest that zebrafish can be used as an animal model to study the biological functions of human CA8 in vivo.
authors.affiliation: Department of Life Science, Tunghai University, Taichung 407, Taiwan, Republic of China.
authors.forename: Min-Syuan
authors.initials: MS
authors.lastname: Huang
authors.affiliation: Department of Life Science, Tunghai University, Taichung 407, Taiwan, Republic of China.
authors.forename: Tze-Kai
authors.initials: TK
authors.lastname: Wang
authors.affiliation: Department of Life Science, Tunghai University, Taichung 407, Taiwan, Republic of China; Life Science Research Center, Tunghai University, Taichung 407, Taiwan, Republic of China.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Liu
authors.affiliation: Department of Life Science, Tunghai University, Taichung 407, Taiwan, Republic of China.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Li
authors.affiliation: Department of Life Science, Tunghai University, Taichung 407, Taiwan, Republic of China.
authors.forename: Tang-Hao
authors.initials: TH
authors.lastname: Chi
authors.affiliation: Department of Life Science, Tunghai University, Taichung 407, Taiwan, Republic of China.
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Chou
authors.affiliation: Department of Life Science, Tunghai University, Taichung 407, Taiwan, Republic of China; Life Science Research Center, Tunghai University, Taichung 407, Taiwan, Republic of China. Electronic address: mhsieh@thu.edu.tw.
authors.forename: Mingli
authors.initials: M
authors.lastname: Hsieh
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D002531:Cerebellum / Q000201:enzymology* / Q000473:pathology; D004195:Disease Models, Animal; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009422:Nervous System Diseases / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D009474:Neurons / Q000201:enzymology* / Q000473:pathology; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000096:biosynthesis* / Q000235:genetics; D002256:Carbonic Anhydrases; D047908:Intracellular Signaling Peptides and Proteins
chemical_list: D014408:Biomarkers, Tumor; D009419:Nerve Tissue Proteins; D029961:Zebrafish Proteins; C512821:Car8 protein, mouse; D002256:Carbonic Anhydrases; C512818:CA8 protein, human; D047908:Intracellular Signaling Peptides and Proteins
keywords: Carbonic anhydrase 8; Cell migration and invasion; Cerebellar granule neuron; Neuro-2a; SK-N-SH; Zebrafish