title: In vitro culture for doubled haploids: tools for molecular breeding.
abstract: Doubled haploid (DH) techniques using immature microspores or embryos rescued in vitro following interspecific hybridization are important tools for breeding wheat and barley and for generating populations for gene mapping purposes. Here, we describe methods being used for the successful production of spring wheat and barley DHs in Australia.
authors.affiliation: Department of Agriculture and Food, Government of WA, 3 Baron-Hay Court, South Perth, 6151, WA, Australia, sue.broughton@agric.wa.gov.au.
authors.forename: Sue
authors.initials: S
authors.lastname: Broughton
authors.forename: Parminder K
authors.initials: PK
authors.lastname: Sidhu
authors.forename: Philip A
authors.initials: PA
authors.lastname: Davies
mesh_terms: D001947:Breeding / Q000379:methods*; D032461:Chromosomes, Plant; D046508:Culture Techniques; D032621:DNA Shuffling; D035264:Flowers / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D006238:Haploidy; D001467:Hordeum / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D014908:Triticum / Q000166:cytology / Q000235:genetics / Q000254:growth & development