title: TGFBI, CHST6, and GSN gene analysis in Mexican patients with stromal corneal dystrophies.
abstract: OBJECTIVE
  The purpose of our study was to describe the results of molecular screening of TGFBI, CHST6, and GSN genes in a group of Mexican patients with different stromal corneal dystrophies (CD).
  
  
  METHODS
  A total of 16 CD Mexican patients pertaining to nine different pedigrees were subjected to a complete ophthalmological investigation. A clinical diagnosis of lattice CD was performed in 10 patients from five pedigrees. Three patients from two pedigrees were diagnosed with granular CD type 2, two patients with unrelated probands had Finnish-type corneal amyloidosis, and one patient had macular CD. Genetic analysis included DNA isolation from blood leukocytes and polymerase chain reaction (PCR) amplification and direct nucleotide sequencing of TGFBI, CHST6, and GSN genes.
  
  
  RESULTS
  Seven lattice CD patients from four unrelated families had an identical p.H626R mutation in TGFBI, three patients from a single lattice CD family carried a p.R124C substitution in TGFBI, and a granular type 2 CD pedigree was demonstrated to carry a heterozygous TGFBI p.M619K substitution. A patient having Finnish-type corneal amyloidosis had a p.D187N mutation in GSN. Finally, molecular analysis of CHST6 in a patient with macular CD disclosed the presence of a homozygous p.Y110C change.
  
  
  CONCLUSIONS
  This study improves the knowledge of the genetic features of Mexican patients with corneal stromal dystrophies by identifying mutations in the TGFBI, CHST6, and GSN genes. Genetic screening of larger samples of patients from distinct ethnic groups would be of great importance for a better understanding of the mutational spectrum of stromal CD.
authors.affiliation: Department of Genetics-Research Unit, Institute of Ophthalmology "Conde de Valenciana", Mexico City, Mexico.
authors.forename: Johanna
authors.initials: J
authors.lastname: Gonzalez-Rodriguez
authors.forename: Arturo
authors.initials: A
authors.lastname: Ramirez-Miranda
authors.forename: Sergio E
authors.initials: SE
authors.lastname: Hernandez-Da Mota
authors.forename: Juan C
authors.initials: JC
authors.lastname: Zenteno
mesh_terms: D000328:Adult; D003317:Corneal Dystrophies, Hereditary / Q000453:epidemiology / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D005260:Female; D018260:Gelsolin / Q000235:genetics*; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D009154:Mutation; D010375:Pedigree; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015238:Sulfotransferases / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics*; D055815:Young Adult; D000097811:Carbohydrate Sulfotransferases; D000099222:betaIG-H3 Protein
chemical_list: D016326:Extracellular Matrix Proteins; D018260:Gelsolin; D015238:Sulfotransferases; D016212:Transforming Growth Factor beta; D000097811:Carbohydrate Sulfotransferases; D000099222:betaIG-H3 Protein