title: A novel adenovirus in Chinstrap penguins (Pygoscelis antarctica) in Antarctica.
abstract: Adenoviruses (family Adenoviridae) infect various organ systems and cause diseases in a wide range of host species. In this study, we examined multiple tissues from Chinstrap penguins (Pygoscelis antarctica), collected in Antarctica during 2009 and 2010, for the presence of novel adenoviruses by PCR. Analysis of a 855-bp region of the hexon gene of a newly identified adenovirus, designated Chinstrap penguin adenovirus 1 (CSPAdV-1), showed nucleotide (amino acid) sequence identity of 71.8% (65.5%) with South Polar skua 1 (SPSAdV-1), 71% (70%) with raptor adenovirus 1 (RAdV-1), 71.4% (67.6%) with turkey adenovirus 3 (TAdV-3) and 61% (61.6%) with frog adenovirus 1 (FrAdV-1). Based on the genetic and phylogenetic analyses, CSPAdV-1 was classified as a member of the genus, Siadenovirus. Virus isolation attempts from kidney homogenates in the MDTC-RP19 (ATCC® CRL-8135™) cell line were unsuccessful. In conclusion, this study provides the first evidence of new adenovirus species in Antarctic penguins.
authors.affiliation: Department of Microbiology, College of Medicine, Institute for Viral Diseases, Korea University, Seoul 136-705, Korea. sylee163@gmail.com.
authors.forename: Sook-Young
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Division of Life Sciences, Korea Polar Research Institute, Incheon 406-840, Korea. jhkim94@kopri.re.kr.
authors.forename: Jeong-Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Microbiology, College of Medicine, Institute for Viral Diseases, Korea University, Seoul 136-705, Korea. suerte8422@hotmail.com.
authors.forename: Yon Mi
authors.initials: YM
authors.lastname: Park
authors.affiliation: Department of Biomedical Science, College of Medicine, Korea University, Seoul 136-705, Korea. oshin@korea.ac.kr.
authors.forename: Ok Sarah
authors.initials: OS
authors.lastname: Shin
authors.affiliation: Department of Pathology, College of Medicine, Korea University, Guro Hospital, Seoul 152-703, Korea. sswords@naver.com.
authors.forename: Hankyeom
authors.initials: H
authors.lastname: Kim
authors.affiliation: Division of Life Sciences, Korea Polar Research Institute, Incheon 406-840, Korea. hchoi82@kopri.re.kr.
authors.forename: Han-Gu
authors.initials: HG
authors.lastname: Choi
authors.affiliation: Department of Microbiology, College of Medicine, Institute for Viral Diseases, Korea University, Seoul 136-705, Korea. jwsong@korea.ac.kr.
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Song
mesh_terms: D000257:Adenoviridae Infections / Q000662:veterinary* / Q000821:virology; D000825:Animal Structures / Q000821:virology; D000818:Animals; D000864:Antarctic Regions; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D046809:Siadenovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D046429:Spheniscidae / Q000821:virology*
chemical_list: D004279:DNA, Viral