title: Expression of Hsp90α and cyclin B1 were related to prognosis of esophageal squamous cell carcinoma and keratin pearl formation.
abstract: Hsp90α (heat shock protein 90α), one of the important molecular chaperones in cancer cell signal transduction, has been a new candidate target for cancer therapy. Cyclin B1, the client protein of Hsp90α, plays a key role as a mitotic cyclin in the G2-M phase transition during the cell cycle progression. However, the relationship between the level of HSP90α and cyclin B1, the location of Hsp90α and cyclin B1 in prognosis of esophageal squamous cell carcinoma (ESCC) has not been examined. Here, we demonstrate that the diagnostic significance of Hsp90α and cyclin B1 by immunohistochemistry and the association of Hsp90α and cyclin B1 expression in ESCC. In the specimens from 105 ESCC patients (81 stained with Hsp90α antibody by Immunohistochemistry, 65 with cyclin B1 antibody, and among them, 41 paired specimens were stained with Hsp90α and cyclin B1 respectively, and then checked for the correlation of the level and location of Hsp90α and cylcin B1. The positivity rate of Hsp90α and cyclin B1 expression were 96.3% (78 of 81) and 84.6% (55 of 65) respectively. Both of them, the expression levels are associated with the clinical pathological stage (Hsp90α, p=0.027; cyclin B1, p=0.007). No association was found between Hsp90α or cyclin B1 and gender, age, tumor location. As to TMN stage, there is no association with the level of Hsp90α, However, cyclin B1 expression is significantly related to tumor status (p=0.002). Interestingly, Hsp90α expression was negatively correlated to cyclin B1 expression (Gamma=-0.692, p=0.007) in the keratin pearls though there is a positive correlation in the other areas of tumor (Gamma=0.503, p=0.015), which suggest Hsp90α might play diverse roles in the cyclin B1 expression and cyclin B1 related cell cycle regulation in the different area of tumor. These findings demonstrated that the expression of Hsp90α, cyclin B1 protein is associated with tumor malignancy and prognosis for patients with human esophageal squamous cell carcinoma, and Hsp90α might be involved in cyclin B1 expression regulation and cell cycle regulation in keratin peal formation of ESCC.
authors.affiliation: Department of Occupational Medicine and Health, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Tingyuan
authors.initials: T
authors.lastname: Huang
authors.affiliation: Department of Oncology, The First Affiliated Hosipital of Guangdong Pharmaceutical University Guangzhou, Guangdong, China.
authors.forename: Size
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Clinical Nutrition, Sun Yat-Sen University Guangzhou, Guangdong, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Han
authors.affiliation: Department of Occupational Medicine and Health, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Huadan
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Occupational Medicine and Health, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Zhizhou
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Occupational Medicine and Health, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Occupational Medicine and Health, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Qiangbin
authors.initials: Q
authors.lastname: Yin
authors.affiliation: Department of Occupational Medicine and Health, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Occupational Medicine and Health, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Occupational Medicine and Health, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Peijuan
authors.initials: P
authors.lastname: Dai
authors.affiliation: Department of Occupational Medicine and Health, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Danping
authors.initials: D
authors.lastname: Duan
authors.affiliation: Department of Occupational Medicine and Health, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
authors.affiliation: Department of Occupational Medicine and Health, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism / Q000401:mortality; D056744:Cyclin B1 / Q000235:genetics / Q000378:metabolism*; D022083:Cytoplasmic Structures / Q000473:pathology*; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000401:mortality; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D007633:Keratins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012189:Retrospective Studies; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D056744:Cyclin B1; D018841:HSP90 Heat-Shock Proteins; C533585:HSP90AA2P protein, human; D007633:Keratins
keywords: Hsp90α; cyclin B1; esophageal squamous cell carcinoma; immunohistochemistry; keratin pearl; prognosis