title: Nature and extent of left/right axis defects in T(Wis) /T(Wis) mutant mouse embryos.
abstract: BACKGROUND
  Mutations in the T-box gene Brachyury have well known effects on invagination of the endomesodermal layer during gastrulation, but the gene also plays a role in the determination of left/right axis determination that is less well studied. Previous work has implicated node morphology in this effect. We use the T(Wis) allele of Brachyury to investigate the molecular and morphological effects of the T locus on axis determination in the mouse.
  
  
  RESULTS
  Similar to embryos mutant for the T allele, T(Wis) /T(Wis) embryos have a high incidence of ventral and/or reversed heart looping. In addition, heterotaxia between the direction of heart looping and the direction of embryo turning is common. Scanning electron microscopy reveals defects in node morphology including irregularity, smaller size, and a decreased number of cilia, although the cilia appear morphologically normal. Molecular analysis shows a loss of perinodal expression of genes involved in Nodal signaling, namely Cer2, Gdf1, and Nodal itself. There is also loss of Dll1 expression, a key component of the Notch signaling pathway, in the presomitic mesoderm.
  
  
  CONCLUSIONS
  Morphological abnormalities of the node as well as disruptions of the molecular cascade of left/right axis determination characterize T(Wis) /T(Wis) mutants. Decreased Notch signaling may account for both the morphological defects and the absence of expression of genes in the Nodal signaling pathway.
authors.affiliation: Department of Genetics and Development, Columbia University Medical Center, New York, New York.
authors.forename: Daniel
authors.initials: D
authors.lastname: Concepcion
authors.forename: Virginia E
authors.initials: VE
authors.lastname: Papaioannou
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D002135:Calcium-Binding Proteins; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D055452:Growth Differentiation Factor 1 / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D055457:Nodal Protein / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy
chemical_list: D002135:Calcium-Binding Proteins; C512925:Dlk1 protein, mouse; C525978:Gdf1 protein, mouse; D055452:Growth Differentiation Factor 1; D036341:Intercellular Signaling Peptides and Proteins; D055457:Nodal Protein
keywords: Brachyury; Cer2; Dll1; Gdf1; Left/right asymmetry; Nodal; TWis; embryonic node