title: Effects of the water-holding capability of polyvinyl formal sponges on osteogenic ability in in vivo experiments.
abstract: In this study, dextran-coated polyvinyl formal (PVF) sponges with high water-holding capability were developed to increase the osteogenic response in the PVF sponge. The study aimed to estimate the effect of the increased water-holding capability of the sponges on osteogenic capacity at a bone defect site in the rabbit femur epiphysis. Bone formation was evaluated using radiography, microcomputed tomography (CT), and histological analysis at 2, 4, and 6 weeks after implantation. As shown by radiography and micro-CT findings, the dextran-coated PVF sponge without water-holding capability showed little bone formation at all evaluated time points. However, the dextran-coated PVF sponge with high water-holding capability showed increasing bone formation around the implant at 4 and 6 weeks after implantation. Furthermore, as shown by micro-CT quantitative analysis, the grafted PVF sponge with high water-holding capability showed significantly greater values for percentage of bone volume per total volume and mean bone mineral density compared with the grafted PVF sponge without water-holding capability at 4 and 6 weeks after implantation. These results suggest that the dextran-coated PVF sponge with high water-holding capability promoted osteogenesis in vivo. The PVF sponge might be a new biomaterial to be used as a fill material for bone defects.
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, Chuo-ku, Kumamoto, Japan.
authors.forename: Wakana
authors.initials: W
authors.lastname: Togami
authors.forename: Akira
authors.initials: A
authors.lastname: Sei
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Okada
authors.forename: Takuya
authors.initials: T
authors.lastname: Taniwaki
authors.forename: Toru
authors.initials: T
authors.lastname: Fujimoto
authors.forename: Shogo
authors.initials: S
authors.lastname: Tahata
authors.forename: Kazuma
authors.initials: K
authors.lastname: Nagamura
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nakanishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuta
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D003911:Dextrans / Q000737:chemistry / Q000494:pharmacology; D004838:Epiphyses / Q000000981:diagnostic imaging / Q000293:injuries; D005269:Femur / Q000000981:diagnostic imaging / Q000293:injuries*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D011145:Polyvinyls / Q000737:chemistry / Q000494:pharmacology; D011817:Rabbits; D013997:Time Factors; D055114:X-Ray Microtomography
chemical_list: D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D003911:Dextrans; D011145:Polyvinyls
keywords: bone tissue engineering; coated polyvinyl formal sponge; high water-holding capability; microcomputed tomography; osteogenic ability