title: The Aβ peptide forms non-amyloid fibrils in the presence of carbon nanotubes.
abstract: Carbon nanotubes have specific properties that make them potentially useful in biomedicine and biotechnology. However, carbon nanotubes may themselves be toxic, making it imperative to understand how carbon nanotubes interact with biomolecules such as proteins. Here, we used NMR, CD, and ThT/fluorescence spectroscopy together with AFM imaging to study pH-dependent molecular interactions between single walled carbon nanotubes (SWNTs) and the amyloid-beta (Aβ) peptide. The aggregation of the Aβ peptide, first into oligomers and later into amyloid fibrils, is considered to be the toxic mechanism behind Alzheimer's disease. We found that SWNTs direct the Aβ peptides to form a new class of β-sheet-rich yet non-amyloid fibrils.
authors.affiliation: Gorlaeus Laboratory, Leiden Institute of Chemistry, Leiden University, 2300RA Leiden, The Netherlands. abrahams@chem.leidenuniv.nl.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Luo
authors.forename: Sebastian K T S
authors.initials: SK
authors.lastname: Wärmländer
authors.forename: Chien-Hung
authors.initials: CH
authors.lastname: Yu
authors.forename: Kamran
authors.initials: K
authors.lastname: Muhammad
authors.forename: Astrid
authors.initials: A
authors.lastname: Gräslund
authors.forename: Jan
authors.initials: J
authors.lastname: Pieter Abrahams
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000648:ultrastructure*; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000648:ultrastructure*; D001665:Binding Sites; D008422:Materials Testing; D046912:Multiprotein Complexes / Q000737:chemistry / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure*; D010446:Peptide Fragments / Q000737:chemistry* / Q000648:ultrastructure*; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D046912:Multiprotein Complexes; D037742:Nanotubes, Carbon; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40)