title: Engineered tumor cell apoptosis monitoring method based on dynamic laser tweezers.
abstract: Monitoring the cells' apoptosis progression could provide a valuable insight into the temporal events that initiate cell death as well as the potential for rescue of apoptotic cells. In this paper, we engineered a novel and robust method for monitoring apoptosis of tumor cells based on dynamic laser tweezers, using A549 and HeLa cell line as typical samples. The entire experiment can be completed in a few hours with small amount of fluid sample, presenting great advantages of celerity, microscaled measurement, and label-free explorations without perturbing experimental conditions in combination with other probes. Validity and stability of this method are verified experimentally in terms of physical parameters of the system. The proposed technique has great potential in improving cancer treatment by monitoring the objective efficacy of tumor cell killing.
authors.affiliation: Key Laboratory of Optoelectronic Information Science & Technology, Institute of Modern Optics, Nankai University, Ministry of Education of China, Tianjin 300071, China.
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Oncology, Tianjin Union Medicine Centre, Tianjin 300121, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Optoelectronic Information Science & Technology, Institute of Modern Optics, Nankai University, Ministry of Education of China, Tianjin 300071, China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Min
authors.affiliation: Institute of Oncology, Tianjin Union Medicine Centre, Tianjin 300121, China.
authors.forename: Siwei
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Optics Research Group, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands.
authors.forename: H Paul
authors.initials: HP
authors.lastname: Urbach
authors.affiliation: Institute of Micro & Nano Optics, Shenzhen University, Shenzhen 518060, China.
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Yuan
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000008:administration & dosage*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D052898:Optical Tweezers
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin