title: Testing the capability of a polynomial-modified gaussian model in the description and simulation of chromatographic peaks of amlodipine and its impurity in ion-interaction chromatography.
abstract: In this paper, the capability of a polynomial-modified Gaussian model to relate the peak shape of basic analytes, amlodipine, and its impurity A, with the change of chromatographic conditions was tested. For the accurate simulation of real chromatographic peaks the authors proposed the three-step procedure based on indirect modeling of peak width at 10% of peak height (W0.1), individual values of left-half width (A) and right-half width (B), number of theoretical plates (N), and tailing factor (Tf). The values of retention factors corresponding to the peak beginning (k(B)), peak apex (k(A)), peak ending (k(E)), and peak heights (H0) of the analytes were directly modeled. Then, the investigated experimental domain was divided to acquire a grid of appropriate density, which allowed the subsequent calculation of W0.1, A, B, N, and Tf. On the basis of the predicted results for Tf and N, as well as the defined criteria for the simulation the following conditions were selected: 33% acetonitrile/67% aqueous phase (55 mM perchloric acid, pH 2.2) at 40°C column temperature. Perfect agreement between predicted and experimental values was obtained confirming the ability of polynomial modified Gaussian model and three-step procedure to successfully simulate the real chromatograms in ion-interaction chromatography.
authors.affiliation: Department of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Colović
authors.forename: Ana
authors.initials: A
authors.lastname: Vemić
authors.forename: Nađa
authors.initials: N
authors.lastname: Kostić
authors.forename: Anđelija
authors.initials: A
authors.lastname: Malenović
authors.forename: Mirjana
authors.initials: M
authors.lastname: Medenica
mesh_terms: D017311:Amlodipine / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000295:instrumentation*; D015233:Models, Statistical
chemical_list: D017311:Amlodipine
keywords: Indirect modeling; Ion-interaction chromatography; Modified Gaussian model; Peak shape simulation