title: [Study on in vitro biomineralization of enamel-binding peptide].
abstract: We present the binding ability of a new peptide (CMPQVMPMC-) with dental enamel after being evaluated in the present study. Under a standard procedure, the recovery of M13 filamentous phage was greatly enhanced by displaying the peptide in phage coat protein p III. Then the cyclic peptide was synthesized using a solid method. The effect of the cyclic peptide in vitro biomineralization was tested in a single-diffusion microtiter plate gel system. Absorbance at 405 nm of each sample was recorded for 24 h at every 6 h intervals. The relatively increased values of each sample were expressed as percentages relative to the blank group (100%). The cyclic peptide resulted in a concentration-dependent delayed nucleation. In addition, the overall values of peptide groups at the end of 24 h were lower than those in the control group but much higher than those in the BSA control group.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Zhou
authors.initials: Z
authors.lastname: Peng
authors.forename: Jie
authors.initials: J
authors.lastname: Deng
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Mao
mesh_terms: D003743:Dental Enamel / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding; D014074:Tooth Calcification
chemical_list: D010455:Peptides