title: In vitro xanthine oxidase inhibitory and in vivo hypouricemic activity of herbal coded formulation (Gouticin).
abstract: Currently, natural products have been used in treating gouty arthritis and are recognized as xanthine oxidase inhibitors. Current study was designed to evaluate in vitro xanthine oxidase inhibitory potential of Gouticin and its ingredients extracts and in vivo hypouricemic activity of gouticin tablet 500 mg twice daily. Ethanol extracts of Gouticin and its ingredients were evaluated in vitro, at 200, 100, 50, 25 μ g/ml concentrations for xanthine oxidase inhibitory activity. IC(50) values of Gouticin and its ingredients were estimated. Further, in vivo therapeutic effect of Gouticin was investigated in comparison with allopathic medicine (Allopurinol) to treat gout. Total patients were 200 that were divided into test and control group. Herbal coded medicine (Gouticin) was given to test group and allopathic medicine allopurinol was administered to control group. In vitro, Gouticin has the highest percent inhibition at 96% followed by Allopurinol with 93% inhibition. In vivo study, mean serum uric acid level of patients was 4.62 mg/dl and 5.21mg/dl by use of Gouticin and Allopurinol at end of therapy. The study showed that herbal coded formulation gouticin and its ingredients are potential sources of natural xanthine oxidase inhibitors. Gouticin 500 mg twice daily is more effective than the allopurinol 300mg once daily in the management of gout.
authors.affiliation: Department of Eastern Medicine and Surgery, Faculty of Medical and Health Sciences, The University of Poonch, Rawalakot, Azad Jammu & Kashmir, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Akram
authors.affiliation: Faculty of Eastern Medicine and Surgery, Hamdard University Karachi, Pakistan.
authors.forename: Khan
authors.initials: K
authors.lastname: Usmanghani
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Baqai Medical University, Karachi, Pakistan.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ahmed
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Azhar
authors.affiliation: Department of Horticulture, Faculty of Agriculture, The University of Poonch, Rawalakot, Azad Jammu & Kashmir, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Hamid
mesh_terms: D000493:Allopurinol / Q000627:therapeutic use; D005260:Female; D006073:Gout / Q000188:drug therapy*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal; D014527:Uric Acid / Q000097:blood; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
chemical_list: D010936:Plant Extracts; D014527:Uric Acid; D000493:Allopurinol; D014969:Xanthine Oxidase