title: The tissue-specific transcriptomic landscape of the mid-gestational mouse embryo.
abstract: Differential gene expression is a prerequisite for the formation of multiple cell types from the fertilized egg during embryogenesis. Understanding the gene regulatory networks controlling cellular differentiation requires the identification of crucial differentially expressed control genes and, ideally, the determination of the complete transcriptomes of each individual cell type. Here, we have analyzed the transcriptomes of six major tissues dissected from mid-gestational (TS12) mouse embryos. Approximately one billion reads derived by RNA-seq analysis provided extended transcript lengths, novel first exons and alternative transcripts of known genes. We have identified 1375 genes showing tissue-specific expression, providing gene signatures for each of the six tissues. In addition, we have identified 1403 novel putative long noncoding RNA gene loci, 439 of which show differential expression. Our analysis provides the first complete transcriptome data for the mouse embryo. It offers a rich data source for the analysis of individual genes and gene regulatory networks controlling mid-gestational development.
authors.affiliation: Department of Developmental Genetics, Max-Planck-Institute for Molecular Genetics, Ihnestrasse 63-73, 14195 Berlin, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Werber
authors.affiliation: Department of Developmental Genetics, Max-Planck-Institute for Molecular Genetics, Ihnestrasse 63-73, 14195 Berlin, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Wittler
authors.affiliation: Sequencing Core Facility, Max-Planck-Institute for Molecular Genetics, Ihnestrasse 63-73, 14195 Berlin, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Timmermann
authors.affiliation: Department of Developmental Genetics, Max-Planck-Institute for Molecular Genetics, Ihnestrasse 63-73, 14195 Berlin, Germany grote@molgen.mpg.de herrmann@molgen.mpg.de.
authors.forename: Phillip
authors.initials: P
authors.lastname: Grote
authors.affiliation: Department of Developmental Genetics, Max-Planck-Institute for Molecular Genetics, Ihnestrasse 63-73, 14195 Berlin, Germany Institute for Medical Genetics, Charité - University Medicine Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12203 Berlin, Germany grote@molgen.mpg.de herrmann@molgen.mpg.de.
authors.forename: Bernhard G
authors.initials: BG
authors.lastname: Herrmann
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000235:genetics*; D005091:Exons; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D059014:High-Throughput Nucleotide Sequencing; D051379:Mice; D008810:Mice, Inbred C57BL; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA; D014018:Tissue Distribution; D059467:Transcriptome
chemical_list: D022661:RNA, Untranslated
keywords: Embryogenesis; Long noncoding RNA; Mouse; RNA-seq; Tissue-specific expression; Transcriptome