title: A population of glomerular glutamatergic neurons controls sensory information transfer in the mouse olfactory bulb.
abstract: In sensory systems, peripheral organs convey sensory inputs to relay networks where information is shaped by local microcircuits before being transmitted to cortical areas. In the olfactory system, odorants evoke specific patterns of sensory neuron activity that are transmitted to output neurons in olfactory bulb (OB) glomeruli. How sensory information is transferred and shaped at this level remains still unclear. Here we employ mouse genetics, 2-photon microscopy, electrophysiology and optogenetics, to identify a novel population of glutamatergic neurons (VGLUT3+) in the glomerular layer of the adult mouse OB as well as several of their synaptic targets. Both peripheral and serotoninergic inputs control VGLUT3+ neurons firing. Furthermore, we show that VGLUT3+ neuron photostimulation in vivo strongly suppresses both spontaneous and odour-evoked firing of bulbar output neurons. In conclusion, we identify and characterize here a microcircuit controlling the transfer of sensory information at an early stage of the olfactory pathway.
authors.affiliation: Department of Basic Neurosciences, School of Medicine, University of Geneva, 1 rue Michel-Servet, 1211, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Switzerland.
authors.forename: Roberta
authors.initials: R
authors.lastname: Tatti
authors.affiliation: Department of Basic Neurosciences, School of Medicine, University of Geneva, 1 rue Michel-Servet, 1211, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Switzerland.
authors.forename: Khaleel
authors.initials: K
authors.lastname: Bhaukaurally
authors.affiliation: Department of Basic Neurosciences, School of Medicine, University of Geneva, 1 rue Michel-Servet, 1211, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Switzerland.
authors.forename: Olivier
authors.initials: O
authors.lastname: Gschwend
authors.affiliation: Departments of Neurobiology and Otolaryngology, School of Medicine, University of Pittsburgh, 3501 Fifth Ave BST3 6058, Pittsburgh, Pennsylvania, 15260.
authors.forename: Rebecca P
authors.initials: RP
authors.lastname: Seal
authors.affiliation: Department of Physiology and Neurology, School of Medicine, University of California, 600 16th Street, San Francisco, California 94143.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Edwards
authors.affiliation: Geneva Neuroscience Center, University of Geneva, Switzerland.|Department of Genetics and Evolution, University of Geneva, 30 quai Ernest-Ansermet, Geneva, Switzerland.
authors.forename: Ivan
authors.initials: I
authors.lastname: Rodriguez
authors.affiliation: Department of Basic Neurosciences, School of Medicine, University of Geneva, 1 rue Michel-Servet, 1211, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Switzerland.
authors.forename: Alan
authors.initials: A
authors.lastname: Carleton
mesh_terms: D026922:Amino Acid Transport Systems, Acidic / Q000502:physiology*; D000818:Animals; D055724:Electrophysiological Phenomena / Q000502:physiology*; D059330:GABAergic Neurons / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009812:Odorants; D009830:Olfactory Bulb / Q000502:physiology*; D009833:Olfactory Pathways; D055696:Olfactory Perception / Q000502:physiology*; D012677:Sensation / Q000502:physiology; D011984:Sensory Receptor Cells / Q000502:physiology
chemical_list: D026922:Amino Acid Transport Systems, Acidic; C504941:Slc17a8 protein, mouse