title: The Plant Membrane-Associated REMORIN1.3 Accumulates in Discrete Perihaustorial Domains and Enhances Susceptibility to Phytophthora infestans.
abstract: Filamentous pathogens such as the oomycete Phytophthora infestans infect plants by developing specialized structures termed haustoria inside the host cells. Haustoria are thought to enable the secretion of effector proteins into the plant cells. Haustorium biogenesis, therefore, is critical for pathogen accommodation in the host tissue. Haustoria are enveloped by a specialized host-derived membrane, the extrahaustorial membrane (EHM), which is distinct from the plant plasma membrane. The mechanisms underlying the biogenesis of the EHM are unknown. Remarkably, several plasma membrane-localized proteins are excluded from the EHM, but the remorin REM1.3 accumulates around P. infestans haustoria. Here, we used overexpression, colocalization with reporter proteins, and superresolution microscopy in cells infected by P. infestans to reveal discrete EHM domains labeled by REM1.3 and the P. infestans effector AVRblb2. Moreover, SYNAPTOTAGMIN1, another previously identified perihaustorial protein, localized to subdomains that are mainly not labeled by REM1.3 and AVRblb2. Functional characterization of REM1.3 revealed that it is a susceptibility factor that promotes infection by P. infestans. This activity, and REM1.3 recruitment to the EHM, require the REM1.3 membrane-binding domain. Our results implicate REM1.3 membrane microdomains in plant susceptibility to an oomycete pathogen.
authors.affiliation: Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom (T.O.B., A.R., Y.F.D., S.K., S.R.);Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom (T.O.B.);John Innes Centre, Norwich NR4 7UH, United Kingdom (A.R.);Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200 Centre National de la Recherche Scientifique-Université Bordeaux Segalen-Institut National de la Recherche Agronomique, F-33883 Villenave d'Ornon cedex, France (S.M.); andLaboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441 Institut National de la Recherche Agronomique-Unité Mixte de Recherche 2594 Centre National de la Recherche Scientifique, F-31326 Castanet-Tolosan, France (S.R.).
authors.forename: Tolga O
authors.initials: TO
authors.lastname: Bozkurt
authors.affiliation: Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom (T.O.B., A.R., Y.F.D., S.K., S.R.);Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom (T.O.B.);John Innes Centre, Norwich NR4 7UH, United Kingdom (A.R.);Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200 Centre National de la Recherche Scientifique-Université Bordeaux Segalen-Institut National de la Recherche Agronomique, F-33883 Villenave d'Ornon cedex, France (S.M.); andLaboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441 Institut National de la Recherche Agronomique-Unité Mixte de Recherche 2594 Centre National de la Recherche Scientifique, F-31326 Castanet-Tolosan, France (S.R.).
authors.forename: Annis
authors.initials: A
authors.lastname: Richardson
authors.affiliation: Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom (T.O.B., A.R., Y.F.D., S.K., S.R.);Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom (T.O.B.);John Innes Centre, Norwich NR4 7UH, United Kingdom (A.R.);Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200 Centre National de la Recherche Scientifique-Université Bordeaux Segalen-Institut National de la Recherche Agronomique, F-33883 Villenave d'Ornon cedex, France (S.M.); andLaboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441 Institut National de la Recherche Agronomique-Unité Mixte de Recherche 2594 Centre National de la Recherche Scientifique, F-31326 Castanet-Tolosan, France (S.R.).
authors.forename: Yasin F
authors.initials: YF
authors.lastname: Dagdas
authors.affiliation: Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom (T.O.B., A.R., Y.F.D., S.K., S.R.);Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom (T.O.B.);John Innes Centre, Norwich NR4 7UH, United Kingdom (A.R.);Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200 Centre National de la Recherche Scientifique-Université Bordeaux Segalen-Institut National de la Recherche Agronomique, F-33883 Villenave d'Ornon cedex, France (S.M.); andLaboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441 Institut National de la Recherche Agronomique-Unité Mixte de Recherche 2594 Centre National de la Recherche Scientifique, F-31326 Castanet-Tolosan, France (S.R.).
authors.forename: Sébastien
authors.initials: S
authors.lastname: Mongrand
authors.affiliation: Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom (T.O.B., A.R., Y.F.D., S.K., S.R.);Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom (T.O.B.);John Innes Centre, Norwich NR4 7UH, United Kingdom (A.R.);Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200 Centre National de la Recherche Scientifique-Université Bordeaux Segalen-Institut National de la Recherche Agronomique, F-33883 Villenave d'Ornon cedex, France (S.M.); andLaboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441 Institut National de la Recherche Agronomique-Unité Mixte de Recherche 2594 Centre National de la Recherche Scientifique, F-31326 Castanet-Tolosan, France (S.R.).
authors.forename: Sophien
authors.initials: S
authors.lastname: Kamoun
authors.affiliation: Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom (T.O.B., A.R., Y.F.D., S.K., S.R.);Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom (T.O.B.);John Innes Centre, Norwich NR4 7UH, United Kingdom (A.R.);Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200 Centre National de la Recherche Scientifique-Université Bordeaux Segalen-Institut National de la Recherche Agronomique, F-33883 Villenave d'Ornon cedex, France (S.M.); andLaboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441 Institut National de la Recherche Agronomique-Unité Mixte de Recherche 2594 Centre National de la Recherche Scientifique, F-31326 Castanet-Tolosan, France (S.R.) sylvain.raffaele@toulouse.inra.fr.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Raffaele