title: Multilocus sequence typing (MLST) of Listeria monocytogenes.
abstract: Nucleotide sequence-based methods focusing on the single nucleotide polymorphisms (SNPs) of Listeria monocytogenes housekeeping genes facilitate the rapid and interlaboratory comparison on open accessible databases, such as the multilocus sequence typing (MLST) databases that are available. MLST has advantages over other methods as it can reconstruct ancestral and evolutionary linkage between L. monocytogenes isolates. MLST detects all genetic variations within the amplified housekeeping gene that accumulate slowly. This chapter describes how to undertake MLST.
authors.affiliation: Department of Veterinary Public Health and Food Science, Institute of Milk Hygiene, Milk Technology and Food Science, University of Veterinary Medicine, Veterinärplatz 1, 1210, Vienna, Austria, beatrix.stessel@vetmeduni.ac.at.
authors.forename: Beatrix
authors.initials: B
authors.lastname: Stessl
authors.forename: Irene
authors.initials: I
authors.lastname: Rückerl
authors.forename: Martin
authors.initials: M
authors.lastname: Wagner
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods; D020043:Genes, Essential; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics*; D008088:Listeriosis / Q000382:microbiology; D058885:Multilocus Sequence Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004269:DNA, Bacterial