title: LC-MS/MS determination of cinacalcet enantiomers in rat plasma on Chirobiotic V column in polar ionic mode: application to a pharmacokinetic study.
abstract: A simple and selective polar ionic liquid chromatography-tandem mass spectrometric method for separation and determination of cinacalcet enantiomers in rat plasma was developed and validated. The chromatographic separation was accomplished on a Chirobiotic V column packed with vancomycin as a chiral stationary phase using 2.5 mm ammonium formate in 100% methanol as a mobile phase in an isocratic mode of elution at a flow rate of 1.0 mL/min. The analytes were extracted from rat plasma by precipitating the proteins with acetonitrile. The developed method exhibited a linear dynamic range over 0.5-500 ng/mL in rat plasma for both enantiomers. The method was successfully applied to study the pharmacokinetics after a single dose by oral administration of 10 mg/kg of cinacalcet enantiomers to healthy male Wistar rats.
authors.affiliation: Analytical Chemistry Division, Discovery Laboratory, Indian Institute of Chemical Technology, Tarnaka, Hyderabad, 500 607, India.
authors.forename: Nageswara Rao
authors.initials: NR
authors.lastname: Ramisetti
authors.forename: Sravan
authors.initials: S
authors.lastname: Bompelli
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D000069449:Cinacalcet; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008297:Male; D009281:Naphthalenes / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012965:Sodium Chloride; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014640:Vancomycin / Q000737:chemistry
chemical_list: D009281:Naphthalenes; D012965:Sodium Chloride; D014640:Vancomycin; D000069449:Cinacalcet
keywords: cinacalcet; enantiomers; polar ionic; rat plasma; vancomycin