title: Measurement of the temperature of the resting rotor in analytical ultracentrifugation.
abstract: Accurate measurements of rotor temperature are critical for the interpretation of hydrodynamic parameters in analytical ultracentrifugation. We have recently developed methods for a more accurate determination of the temperature of a spinning rotor using iButton temperature loggers. Here we report that the temperature measured with the iButton on the counterbalance of a resting rotor, following thermal equilibration under high vacuum, closely corresponded to the temperature of the spinning rotor with a precision better than 0.2°C. This strategy offers an inexpensive and straightforward approach to monitor the accuracy of the temperature calibration and determine corrective temperature offsets.
authors.affiliation: Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: rodolfo.ghirlando@nih.gov.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Ghirlando
authors.affiliation: Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Huaying
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Bioengineering and Physical Science Shared Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Balbo
authors.affiliation: Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Piszczek
authors.affiliation: Institute for Biophysical Chemistry, Hannover Medical School, 30625 Hannover, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Curth
authors.affiliation: Department of Biophysics, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Chad A
authors.initials: CA
authors.lastname: Brautigam
authors.affiliation: Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: schuckp@mail.nih.gov.
authors.forename: Peter
authors.initials: P
authors.lastname: Schuck
mesh_terms: D002138:Calibration; D057446:Hydrodynamics; D011506:Proteins / Q000032:analysis; D013696:Temperature; D014461:Ultracentrifugation / Q000592:standards
chemical_list: D011506:Proteins
keywords: Hydrodynamics; Sedimentation velocity; Temperature calibration