title: Constituents of the roots of Clausena lansium and their potential anti-inflammatory activity.
abstract: Eight new carbazole alkaloids, claulamines C (1), D (2), and E (5) and clausenalines B-F (3, 4, 6-8), four new coumarins, clausemarins A-D (9-12), and 43 known compounds were isolated from the roots of Clausena lansium. The structures of the new compounds were established on the basis of 2D-NMR spectroscopic analysis, and their absolute configurations were established from their ECD spectra. The configuration of wampetin was revised as E using a NOESY experiment. Most of the isolated compounds were evaluated for their potential anti-inflammatory activity. The results showed that compounds 9, 13-18, and 20-22 exhibited strong inhibition of superoxide anion generation with IC50 values ranging from 1.9 to 8.4 μM, while compounds 18, 19, and 21 inhibited elastase release with IC50 values in the range from 2.0 to 6.9 μM.
authors.affiliation: Department of Chemistry, National Cheng Kung University , Tainan 70101, Taiwan.
authors.forename: De-Yang
authors.initials: DY
authors.lastname: Shen
authors.forename: Yu-Yi
authors.initials: YY
authors.lastname: Chan
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.forename: Shin-Hun
authors.initials: SH
authors.lastname: Juang
authors.forename: Shiow-Chyn
authors.initials: SC
authors.lastname: Huang
authors.forename: Ping-Chung
authors.initials: PC
authors.lastname: Kuo
authors.forename: Tran Dinh
authors.initials: TD
authors.lastname: Thang
authors.forename: E-Jian
authors.initials: EJ
authors.lastname: Lee
authors.forename: Amooru G
authors.initials: AG
authors.lastname: Damu
authors.forename: Tian-Shung
authors.initials: TS
authors.lastname: Wu
mesh_terms: D000058:Accidental Falls; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002227:Carbazoles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D054700:Clausena / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
chemical_list: D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D002227:Carbazoles; D003374:Coumarins