title: New strategy to surface functionalization of polymeric nanoparticles: one-pot synthesis of scFv anti-LDL(-)-functionalized nanocapsules.
abstract: OBJECTIVE
  In general, the surface functionalization of polymeric nanoparticles is carried out by covalently bounding ligands to the nanoparticle surface. This process can cause a lack or decrease of the ligand specificity to its target receptor, besides the need of purification steps. We proposed a ligand-metal-chitosan-lecithin complex as a new strategy to functionalize the surface of biodegradable nanoparticles.
  
  
  METHODS
  One pot synthesis of scFv anti-LDL(-)-functionalized nanocapsules was carried out by self-assembly and interfacial reactions. Particle sizing techniques, lipid peroxidation and molecular recognition by enzyme linked immuno sorbent assays were carried out.
  
  
  RESULTS
  The selected formulation had unimodal size distribution with mean diameter of about 130 nm. The metals in the complex did not enhance the oxidative stress, and the scFv anti-LDL(-)-functionalized nanocapsules recognized LDL(-) and did not react with native LDL indicating the maintenance of the active site of the fragment.
  
  
  CONCLUSIONS
  The one pot synthesis, using the ligand-metal-chitosan-lecithin complex to functionalize the surface of the biodegradable nanocapsules, maintained the active site of the antibody fragment making the device interesting for applications in nanomedicine.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Bender
authors.forename: Marcela F
authors.initials: MF
authors.lastname: Cavalcante
authors.forename: Márcia D
authors.initials: MD
authors.lastname: Adorne
authors.forename: Letícia M
authors.initials: LM
authors.lastname: Colomé
authors.forename: Sílvia S
authors.initials: SS
authors.lastname: Guterres
authors.forename: Dulcinéia S P
authors.initials: DS
authors.lastname: Abdalla
authors.forename: Adriana R
authors.initials: AR
authors.lastname: Pohlmann
mesh_terms: D020134:Catalytic Domain; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000737:chemistry; D054709:Lecithins / Q000737:chemistry; D008024:Ligands; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000276:immunology*; D008670:Metals / Q000737:chemistry; D053769:Nanocapsules / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D057127:Single-Chain Antibodies / Q000737:chemistry* / Q000276:immunology*
chemical_list: D054709:Lecithins; D008024:Ligands; D008077:Lipoproteins, LDL; D008670:Metals; D053769:Nanocapsules; D011108:Polymers; D057127:Single-Chain Antibodies; D048271:Chitosan