title: [Genotyping and polymorphism analysis of Plasmodium falciparum isolates by nest-PCR and PCR-RFLP].
abstract: OBJECTIVE
  To analyze the efficiency and specificity of MSP2 alleles genotyping for Plasmodium falciparum isolates by Nest-PCR and PCR-RFLP.
  
  
  METHODS
  MSP2 alleles from Plasmodium falciparum isolates of Yunnan and Hainan were genotyped by Nest-PCR and PCR-RFLP, respectively, and the efficiency and specificity of the two methods were analyzed.
  
  
  RESULTS
  The conventional Nest-PCR method could detect 79.8% (166/208) alleles of MSP2, and 65.7% (65/99) for 3D7 family, but could not identify the type of any allele. While PCR-RFLP showed 25.3% higher genotyping efficiency than Nest-PCR. Moreover, this method could identify the allele types.
  
  
  CONCLUSIONS
  PCR-RFLP genotyping technique is more efficient and specific than conventional Nest-PCR, and it is a convenient tool in the study on molecular epidemiology of malaria.
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Zhang
authors.forename: Jing-Ying
authors.initials: JY
authors.lastname: Hu
authors.forename: Yue-Xin
authors.initials: YX
authors.lastname: Yang
authors.forename: Wei-Qing
authors.initials: WQ
authors.lastname: Pan
mesh_terms: D000483:Alleles; D002681:China / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D016778:Malaria, Falciparum / Q000453:epidemiology; D010963:Plasmodium falciparum / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length