title: Influence of drying treatments on antioxidant capacity of forage legume leaves.
abstract: This study was aimed to investigate the antioxidant capacities of four common forage legume leaves namely, Arachis pintoi (Pintoi), Calapogonium mucunoides (Calapo), Centrosema pubescens (Centro), and Stylosanthes guanensis (Stylo). Two different drying methods (oven-drying and freeze-drying) were employed and antioxidant activities were determined by DPPH, Ferric Reducing Antioxidant Power (FRAP) and β-carotene bleaching assays. Total phenolic content (TPC) was determined using Folin-Ciocalteu assay. Freeze-dried extract showed the highest antioxidant activities by DPPH (EC50 values 1.17-2.13 mg/ml), FRAP (147.08-246.42 μM of Fe(2+)/g), and β-carotene bleaching (57.11-78.60%) compared to oven drying. Hence, freeze drying treatment could be considered useful in retention of antioxidant activity and phenolic content.
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Science, 43400 UPM Serdang, Malaysia.
authors.forename: Saw Yei
authors.initials: SY
authors.lastname: Sang
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Science, 43400 UPM Serdang, Malaysia.
authors.forename: Fazrina
authors.initials: F
authors.lastname: Jamharee
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Science, 43400 UPM Serdang, Malaysia.
authors.forename: K Nagendra
authors.initials: KN
authors.lastname: Prasad
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Science, 43400 UPM Serdang, Malaysia ; Laboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia.
authors.forename: Azrina
authors.initials: A
authors.lastname: Azlan
authors.affiliation: Institute of Veterinary Malaysia, KM 13, Jalan Batu Pahat, Beg Berkunci 520, 86009 Kluang, Johor Malaysia.
authors.forename: Nurzillah
authors.initials: N
authors.lastname: Maliki
keywords: Antioxidants; Freeze-drying; Legume leaves; Oven-drying; Phenolics