title: Observation of permeability of blood-labyrinth barrier during cytomegalovirus-induced hearing loss.
abstract: OBJECTIVE
  Congenital cytomegalovirus (CMV) infection is the most common infectious cause of sensorineural hearing loss in children. This study aims to investigate the pathogenesis CMV-induced hearing loss from the view of integrity of blood-labyrinth-barrier (BLB).
  
  
  METHODS
  Newborn BALB/c mice were randomly divided into three groups (n=22, respectively): CMV group, control group and normal group. The CMV group and control group were intracerebrally injected with equal volume (15 μl) of murine CMV (MCMV; 10(4)IU/0.1 ml) and PBS, respectively, and normal group did not receive any treatment. After three weeks, auditory-evoked brainstem response was assessed, and permeability of BLB was evaluated by Evans blue method. Means between groups were compared using t-test.
  
  
  RESULTS
  We observed that mice injected with MCMV had a hearing loss and it was connected with the permeability changes of BLB. Besides, using hematoxylin-eosin staining, we noticed hyperaemia in stria vascularis and spiral ligament and bleeding in scala vestibule and scala tympani in CMV group.
  
  
  CONCLUSIONS
  All these data indicated the possible association between CMV-induced hearing loss and BLB dysfunction with the characteristics of inflammation. Our data provide a possible path to investigate the mechanism of CMV-induced hearing damage.
authors.affiliation: Department of Otolaryngology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Xuanyi
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Otolaryngology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, China; Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou 221006, China. Electronic address: oto8558@163.com.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Laboratory of Transplantation Immunity of Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Kailin
authors.initials: K
authors.lastname: Xu
authors.affiliation: Laboratory of Transplantation Immunity of Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Department of Otolaryngology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Caiji
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Basic Education Science of Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Haichen
authors.initials: H
authors.lastname: Niu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001299:Audiometry; D002199:Capillary Permeability; D003051:Cochlea / Q000473:pathology; D004396:Coloring Agents / Q000493:pharmacokinetics; D003586:Cytomegalovirus Infections / Q000150:complications*; D007758:Ear, Inner / Q000098:blood supply*; D005070:Evans Blue / Q000493:pharmacokinetics; D016057:Evoked Potentials, Auditory, Brain Stem; D034381:Hearing Loss / Q000821:virology*; D006470:Hemorrhage / Q000473:pathology; D006940:Hyperemia / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D004396:Coloring Agents; D005070:Evans Blue
keywords: Blood–labyrinth-barrier; Cytomegalovirus; Inflammation; Permeability