title: MiR-152 reduces human umbilical vein endothelial cell proliferation and migration by targeting ADAM17.
abstract: As a cleavage enzyme of precursor TNF-α, the high expression level of ADAM17 in endothelial cells is an important factor in atherosclerosis. In this study, we demonstrate that ADAM17 is the target of miR-152. We found that miR-152 could reduce TNF precursor cleavage and inhibit cell proliferation and migration by targeting ADAM17 in human umbilical vein endothelial cells (HUVECs). Furthermore, the expression pattern of miR-152 and corresponding target ADAM17 was opposite in HUVECs under hypoxic conditions. The levels of circulating miR-152 in AS patient sera were lower than those detected in the sera of normal individuals. Our results indicate that miR-152 may be involved in the development of human atherosclerosis and could be used as diagnostic biomarker or therapeutic target in atherosclerosis.
authors.affiliation: General Hospital of Chinese People's Liberation Army, Beijing 100853, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China.
authors.forename: Aixue
authors.initials: A
authors.lastname: Huang
authors.affiliation: Affiliated Hospital of General Hospital of Chinese People's Liberation Army, Beijing 100048, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China.
authors.forename: Xueting
authors.initials: X
authors.lastname: Su
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ding
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China.
authors.forename: Xingliang
authors.initials: X
authors.lastname: Qin
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China.
authors.forename: Lubin
authors.initials: L
authors.lastname: Hou
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China.
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Ge
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Fang
authors.affiliation: General Hospital of Chinese People's Liberation Army, Beijing 100853, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: General Hospital of Chinese People's Liberation Army, Beijing 100853, PR China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Gao
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China. Electronic address: jannbio@gmail.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China. Electronic address: shaonsh@hotmail.com.
authors.forename: Ningsheng
authors.initials: N
authors.lastname: Shao
mesh_terms: D051722:ADAM Proteins / Q000235:genetics*; D000072198:ADAM17 Protein; D000818:Animals; D050197:Atherosclerosis / Q000097:blood / Q000235:genetics; D001483:Base Sequence; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*
chemical_list: C550856:MIRN152 microRNA, human; D035683:MicroRNAs; D051722:ADAM Proteins; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human
keywords: ADAM17; Atherosclerosis; HUVEC; MiR-152