title: Amount of interleukin-1β and interleukin-1 receptor antagonist in periodontitis and healthy patients.
abstract: OBJECTIVE
  Regulation of interleukin-1β (IL-1β) is a critical element of immune responses in health and disease. Additional research is required to determine the levels of interleukin-1 receptor antagonist (IL-1ra) sufficient to inhibit inteleukine-1-induced responses in periodontium. The aim of this study was to compare the levels of IL-1β, IL-1ra in gingival crevicular fluid samples obtained from periodontitis and healthy patients.
  
  
  METHODS
  The periodontitis group consisted of 50 patients (F-31, M-19) with chronic periodontitis. The control group consisted of 30 periodontally healthy subjects (F-19, M-11). Oral hygiene index-simplified, gingival index, pocket depth, clinical attachment level were measured. The concentrations of interleukins in gingival crevicular fluid were measured by the ELISA technique.
  
  
  RESULTS
  The total amounts of interleukin-1β and IL-1ra were significantly higher in periodontitis group than in the control group (P<0.05). The levels of each cytokine were reported also as moles. In the control group there were 800-times more moles/sample of IL-1ra than IL-1β. In periodontitis group nearly 300-times more moles/sample of IL-1ra was obtained. The significant positive Spearman's rank correlations was identified between both concentrations (R=0.39; P<0.05) and total amounts (R=0.31; P<0.05) of cytokines in the periodontitis group.
  
  
  CONCLUSIONS
  The increased secretion of IL-1ra in periodontitis is not adequate to "curb" the release of IL-1β.
authors.affiliation: Chair and Department of Pharmacology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Jordana 38, 41-808 Zabrze, Poland; Private Dental Practice, Gliwice, Poland. Electronic address: aplg@wp.pl.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Gilowski
authors.affiliation: Chair and Department of Conservative Dentistry with Endodontics, School of Medicine with the Division of Dentistry, Medical University of Silesia, Bytom, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Wiench
authors.affiliation: Chair and Department of Conservative Dentistry with Endodontics, School of Medicine with the Division of Dentistry, Medical University of Silesia, Bytom, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Płocica
authors.affiliation: Chair and Department of Pharmacology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Jordana 38, 41-808 Zabrze, Poland.
authors.forename: Tadeusz Faustyn
authors.initials: TF
authors.lastname: Krzemiński
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000032:analysis*; D053583:Interleukin-1beta / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D010518:Periodontitis / Q000276:immunology*
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta
keywords: Chronic periodontitis; Gingival crevicular fluid; Interleukin-1 receptor antagonist; Interleukin-1β