title: Transcriptional control of ROS homeostasis by KUODA1 regulates cell expansion during leaf development.
abstract: The final size of an organism, or of single organs within an organism, depends on an intricate coordination of cell proliferation and cell expansion. Although organism size is of fundamental importance, the molecular and genetic mechanisms that control it remain far from understood. Here we identify a transcription factor, KUODA1 (KUA1), which specifically controls cell expansion during leaf development in Arabidopsis thaliana. We show that KUA1 expression is circadian regulated and depends on an intact clock. Furthermore, KUA1 directly represses the expression of a set of genes encoding for peroxidases that control reactive oxygen species (ROS) homeostasis in the apoplast. Disruption of KUA1 results in increased peroxidase activity and smaller leaf cells. Chemical or genetic interference with the ROS balance or peroxidase activity affects cell size in a manner consistent with the identified KUA1 function. Thus, KUA1 modulates leaf cell expansion and final organ size by controlling ROS homeostasis.
authors.affiliation: 1] Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany [2] Department of Molecular Biology, Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam-Golm, Germany.
authors.forename: Dandan
authors.initials: D
authors.lastname: Lu
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: 1] Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany [2] Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Staffan
authors.initials: S
authors.lastname: Persson
authors.affiliation: 1] Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany [2] Department of Molecular Biology, Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam-Golm, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Mueller-Roeber
authors.affiliation: 1] Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany [2] Department of Molecular Biology, Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam-Golm, Germany [3] Institute of Biology I, RWTH Aachen University, 52074 Aachen, Germany.
authors.forename: Jos H M
authors.initials: JH
authors.lastname: Schippers
mesh_terms: D017360:Arabidopsis / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry; D002940:Circadian Rhythm; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D009195:Peroxidase / Q000378:metabolism; D018515:Plant Leaves / Q000196:embryology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D044763:Ubiquitin-Conjugating Enzymes
chemical_list: D029681:Arabidopsis Proteins; C000599978:KUODA1 protein, Arabidopsis; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D009195:Peroxidase; D044763:Ubiquitin-Conjugating Enzymes