title: Determination of chloramphenicol and zeranols in pig muscle by immunoaffinity column clean-up and LC-MS/MS analysis.
abstract: An immunoaffinity column clean-up and LC-MS/MS method was successfully developed for simultaneous determination of chloramphenicol, zearalanone, α-zearalanol, β-zearalanol, zearalenone, α-zearalenol and β-zearalenol in pig muscle. The sample was extracted with diethyl ether after enzymatic digestion by β-glucuronidase/sulfatase. The extracted solution was evaporated to dryness and the residue was then dissolved in 1 ml of 50% acetonitrile solution. After filtration and dilution with phosphate buffer solution (PBS), the reconstituted solution was cleaned-up with an IAC-CZ immunoaffinity column and then analysed by HPLC-MS/MS. The established method were validated by linearity (r ≥ 0.9990), precision (RSD ≥ 2.9%), average recovery (74.5-105.0%) and limit of detection (0.04-0.10 μg kg(-1)). The developed method is rapid, reliable, sensitive, accurate and has good applicability for real samples.
authors.affiliation: a College of Pharmacy , Chongqing Medical University , Chongqing , China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.forename: Cunxian
authors.initials: C
authors.lastname: Xi
authors.forename: Guomin
authors.initials: G
authors.lastname: Wang
authors.forename: Dongdong
authors.initials: D
authors.lastname: Chen
authors.forename: Shijia
authors.initials: S
authors.lastname: Ding
mesh_terms: D000818:Animals; D002701:Chloramphenicol / Q000032:analysis*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D034421:Sus scrofa; D053719:Tandem Mass Spectrometry; D019155:Veterinary Drugs / Q000032:analysis*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D019155:Veterinary Drugs; D002701:Chloramphenicol; D015029:Zeranol
keywords: HPLC-MS/MS; chloramphenicol; immunoaffinity column; pig muscle; zeranols