title: Ischemic reperfusion injury-induced oxidative stress and pro-inflammatory mediators in liver transplantation recipients.
abstract: OBJECTIVE
  Liver ischemic reperfusion injury is harmful to transplant recipients, and is associated with postoperative morbidity and mortality. Our study was designed to investigate the oxidative stress and pro-inflammatory mediators in liver transplant recipients.
  
  
  METHODS
  We prospectively analyzed 14 recipients who underwent liver transplantation by measuring their blood levels of malondialdehyde (MDA) and cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6, at nine time points perioperatively. We also evaluated the correlations between oxidative stress (MDA levels) and the characteristics of the recipient or the donated graft.
  
  
  RESULTS
  These parameters significantly increased from 1 minute before reperfusion, and the values peaked within 3 to 30 minutes after reperfusion. On the time point at 5 minutes after reperfusion, the MDA levels which were the highest in the recipients correlated with the values of preoperative direct/and total bilirubin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), model for end-stage liver disease (MELD) score, international normalized ratio (INR), and surgical blood loss.
  
  
  CONCLUSIONS
  The levels of MDA, TNF-α, IL-1β, and IL-6 greatly increased with the ischemic reperfusion insult. Recipients with higher values of preoperative direct/and total bilirubin, AST, ALT, MELD score, INR, and surgical blood loss tended to have higher levels of MDA and may suffer more injury from this insult.
authors.affiliation: Department of Anesthesiology, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; College of Medicine, Chang Gung University, Tao-Yuan, Taiwan; Graduate Institute of Clinical Medical Sciences, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Y-F
authors.initials: YF
authors.lastname: Tsai
authors.affiliation: Department of Anesthesiology, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; College of Medicine, Chang Gung University, Tao-Yuan, Taiwan; Graduate Institute of Clinical Medical Sciences, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: F-C
authors.initials: FC
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan.
authors.forename: W-C
authors.initials: WC
authors.lastname: Sung
authors.affiliation: Department of Anesthesiology, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; College of Medicine, Chang Gung University, Tao-Yuan, Taiwan; Graduate Institute of Clinical Medical Sciences, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: C-C
authors.initials: CC
authors.lastname: Lin
authors.affiliation: Department of Anesthesiology, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: P C-H
authors.initials: PC
authors.lastname: Chung
authors.affiliation: College of Medicine, Chang Gung University, Tao-Yuan, Taiwan; Division of Transplantation and Liver Surgery, Department of General Surgery, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan.
authors.forename: W-C
authors.initials: WC
authors.lastname: Lee
authors.affiliation: Department of Anesthesiology, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; College of Medicine, Chang Gung University, Tao-Yuan, Taiwan; Graduate Institute of Clinical Medical Sciences, Chang Gung University, Tao-Yuan, Taiwan. Electronic address: yuhp2001@adm.cgmh.org.tw.
authors.forename: H-P
authors.initials: HP
authors.lastname: Yu
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis / Q000601:surgery*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000175:diagnosis / Q000209:etiology*; D018836:Inflammation Mediators / Q000097:blood*; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis / Q000601:surgery*; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis / Q000601:surgery*; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011446:Prospective Studies; D015427:Reperfusion Injury / Q000097:blood / Q000175:diagnosis / Q000209:etiology*; D012720:Severity of Illness Index; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators