title: Colloidal gold-based immunochromatographic strip test compromising optimised combinations of anti-S. suis capsular polysaccharide polyclonal antibodies for detection of Streptococcus suis.
abstract: A rapid diagnosis kit that detects Streptococcus suis (S. suis) antigens from urine with an immunochromatographic stripe (ICS) test was developed in this study. The ICS test was produced using colloidal gold coated with polyclonal antibodies (pAbs) against S. suis. The pAbs were developed from rabbits immunised with S. suis serotype 2 capsular polysaccharides (CPS). Development of the pAbs was investigated to establish their binding to CPS and to determine the maximum sensitivity of two combination antibodies for the ICS test. The results of the ICS optimisation revealed that the combinations of pAb C-N1 and pAb C-N2 had the highest sensitivity to CPS. The minimum limitation of ICS sensitivity indicated 1.0 × 10(4) colony forming units (CFU) and a CPS concentration of 0.05 µg. The assay time for detection of S. suis antigens is less than 15 min, which is suitable for rapid detection. A cross-reactive test was also conducted, and it detected no other bacteria (Streptococcus pneumoniae, Streptococcus agalactiae, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae). The cross-reactivity of other serotypes in S. suis was also investigated, and tests for serotypes of 1, 1/2, 3, 4, 5, 6, 7, 8, 9, 14, and 16 were positive. This study presents the first report of a development of an ICS that enables the quantitative detection of streptococcal antigens. The S. suis ICS provides several advantages over other methods, including the speed and simplicity of use.
authors.affiliation: Global Collaboration Center, Osaka University, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nakayama
authors.affiliation: Department of Microbiology, Harbin Medical University, China.
authors.forename: Jizi
authors.initials: J
authors.lastname: Zhao
authors.affiliation: International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Japan.
authors.forename: Dan
authors.initials: D
authors.lastname: Takeuchi
authors.affiliation: National institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi, Thailand.
authors.forename: Anusack
authors.initials: A
authors.lastname: Kerdsin
authors.affiliation: Phayao Provincial Hospital, Thailand.
authors.forename: Piphat
authors.initials: P
authors.lastname: Chiranairadul
authors.affiliation: Chaiang Khum General Hospital, Phayao, Thailand.
authors.forename: Prasanee
authors.initials: P
authors.lastname: Areeratana
authors.affiliation: Chaiang Khum General Hospital, Phayao, Thailand.
authors.forename: Phacharaphan
authors.initials: P
authors.lastname: Loetthong
authors.affiliation: Chaiang Khum General Hospital, Phayao, Thailand.
authors.forename: Anupong
authors.initials: A
authors.lastname: Pienpringam
authors.affiliation: International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akeda
authors.affiliation: Infectious Disease Surveillance Center National Institute of Infectious Disease, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Oishi
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016667:Bacterial Capsules / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002846:Chromatography, Affinity / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D011817:Rabbits; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017011:Streptococcus suis / Q000276:immunology* / Q000302:isolation & purification*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D011934:Reagent Strips; C028096:capsular polysaccharide, Streptococcus suis type 2
keywords: Capsular polysaccharides; Colloidal gold; Immunochromatographic stripe; Polyclonal antibody; Streptococcus suis