title: Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis.
abstract: LncRNA SPRY4-IT1 has been shown to promote the progression of melanoma. However, the role of lncRNA SPRY4-IT1 in human esophageal squamous cell carcinoma (ESCC) remains unclear. The purpose of this study is to investigate the clinical significance and biological functions of SPRY4-IT1 in ESCC. The expression levels of lncRNA SPRY4-IT in 92 ESCC patients and 8 ESCC cell lines were evaluated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The prognostic significance was evaluated using Kaplan-Meier and Cox regression analyses. Small interfering RNA (siRNA) was used to suppress SPRY4-IT1 expression in ESCC cell lines. Both in vitro and in vivo assays were performed to further explore its role in tumor progression. SPRY4-IT1 levels were significantly higher in ESCC tissues and cells than in corresponding adjacent noncancerous tissues and nontumorigenic esophageal epithelial cells, and the ESCC patients with higher SPRY4-IT1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY4-IT1 expression. The multivariate analysis revealed that SPRY4-IT1 expression level is an independent prognostic factor in ESCC patients. In vitro assays demonstrated that knockdown of SPRY4-IT1 reduced cell proliferation, invasiveness, and migration. In vivo assays demonstrated that knockdown of SPRY4-IT1 decreases cell growth. SPRY4-IT1 is a novel molecule involved in ESCC progression, which may provide a potential prognostic biomarker and a potential target for therapeutic intervention.
authors.affiliation: Department of Surgical Oncology, Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Hai-Wei
authors.initials: HW
authors.lastname: Xie
authors.forename: Qing-Quan
authors.initials: QQ
authors.lastname: Wu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.forename: Fang-Jun
authors.initials: FJ
authors.lastname: Chen
authors.forename: Lv
authors.initials: L
authors.lastname: Ji
authors.forename: Su-Qing
authors.initials: SQ
authors.lastname: Li
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Wang
authors.forename: Yu-Suo
authors.initials: YS
authors.lastname: Tong
authors.forename: Lei
authors.initials: L
authors.lastname: Tuo
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Lv
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Shi
authors.forename: Xiu-Feng
authors.initials: XF
authors.lastname: Cao
mesh_terms: D000368:Aged; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000401:mortality; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D062085:RNA, Long Noncoding; C499038:SPRY4 protein, human