title: Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon.
abstract: We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes into the Caenorhabditis elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (~60% of injected animals). Genetic and antibiotic markers can be used for selection, and the transposon is active in C. elegans isolates and Caenorhabditis briggsae. We used the miniMos transposon to generate six universal Mos1-mediated single-copy insertion (mosSCI) landing sites that allow targeted transgene insertion with a single targeting vector into permissive expression sites on all autosomes. We also generated two collections of strains: a set of bright fluorescent insertions that are useful as dominant, genetic balancers and a set of lacO insertions to track genome position.
authors.affiliation: 1] Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA. [2] Department of Biology, University of Utah, Salt Lake City, Utah, USA. [3] Danish National Research Foundation Centre for Cardiac Arrhythmia, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Christian
authors.identifier: 0000000231780906
authors.initials: C
authors.lastname: Frøkjær-Jensen
authors.affiliation: 1] Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA. [2] Department of Biology, University of Utah, Salt Lake City, Utah, USA.
authors.forename: M Wayne
authors.initials: MW
authors.lastname: Davis
authors.affiliation: TransgeneOmics, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Mihail
authors.initials: M
authors.lastname: Sarov
authors.affiliation: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Jon
authors.initials: J
authors.lastname: Taylor
authors.affiliation: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Stephane
authors.initials: S
authors.lastname: Flibotte
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, Utah, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: LaBella
authors.affiliation: TransgeneOmics, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Andrei
authors.initials: A
authors.lastname: Pozniakovsky
authors.affiliation: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Donald G
authors.initials: DG
authors.lastname: Moerman
authors.affiliation: 1] Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA. [2] Department of Biology, University of Utah, Salt Lake City, Utah, USA.
authors.forename: Erik M
authors.initials: EM
authors.lastname: Jorgensen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D019295:Computational Biology; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D005819:Genetic Markers / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008957:Models, Genetic; D016254:Mutagenesis, Insertional; D011994:Recombinant Proteins / Q000378:metabolism; D011995:Recombination, Genetic; D019076:Transgenes; D019895:Transposases / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D011994:Recombinant Proteins; C111652:mariner transposases; D049452:Green Fluorescent Proteins; D019895:Transposases