title: Follow-up of implant survival comparing ficoll and bone marrow aspirate concentrate methods for hard tissue regeneration with mesenchymal stem cells in humans.
abstract: OBJECTIVE
  Clinical follow-up of implant survival in 11 patients comparing two different methods for mesenchymal stem cell (MSC) isolation (Ficoll and bone marrow aspirate concentrate [BMAC]) applied in maxillary sinus augmentation.
  
  
  METHODS
  Mononuclear cells, including MSCs, were concentrated with either Ficoll (control group, n=6 sinus) or BMAC (test group, n=12 sinus) and transplanted in combination with bovine bone mineral. A total of 50 implants were placed in a second surgical intervention (17 Ficoll/33 BMAC) and loaded after 4 months. Overall implant survival was assessed with a Kaplan-Meier model using package survival under R.
  
  
  RESULTS
  Implant survival of the Ficoll group was 100% compared with the BMAC group, which had 93.4% survival (95% confidence interval, 0.849-1). The difference between the groups was not significant (p=0.381).
  
  
  CONCLUSIONS
  The BMAC system is an effective and suitable "chair-side" method for clinical application in hard tissue regeneration.
authors.affiliation: Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg , Freiburg, Germany .
authors.forename: Fabian
authors.initials: F
authors.lastname: Duttenhoefer
authors.affiliation: Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg , Freiburg, Germany .
authors.forename: Stefan F
authors.initials: SF
authors.lastname: Hieber
authors.affiliation: Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg , Freiburg, Germany .
authors.forename: Andres
authors.initials: A
authors.lastname: Stricker
authors.affiliation: Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg , Freiburg, Germany .
authors.forename: Rainer
authors.initials: R
authors.lastname: Schmelzeisen
authors.affiliation: Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg , Freiburg, Germany .
authors.forename: Ralf
authors.initials: R
authors.lastname: Gutwald
authors.affiliation: Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg , Freiburg, Germany .
authors.forename: Sebastian
authors.initials: S
authors.lastname: Sauerbier
keywords: biomaterials; cell culture; tissue engineering