title: Rsf-1 overexpression serves as a prognostic marker in human hepatocellular carcinoma.
abstract: Rsf-1 (HBXAP) was recently reported to be overexpressed in various cancers and associated with the malignant behavior of cancer cells. However, the expression of Rsf-1 and its clinical significance in human hepatocellular carcinoma (HCC) have not been reported. In the present study, we analyzed the expression pattern of Rsf-1 in human HCC tissues and found that Rsf-1 was overexpressed in 41.1 % of HCC specimens. There was a significant association between Rsf-1 overexpression and tumor stage (p = 0.0322), AFP (p = 0.0184), and tumor relapse (p = 0.0112). Furthermore, Rsf-1 overexpression correlated with poor overall survival in HCC patients (p < 0.001). Rsf-1 overexpression could serve as an independent predictor for poor recurrence-free survival (p = 0.0079). Small interfering RNA (siRNA) knockdown in SK-Hep-1 cells with high endogenous Rsf-1 expression inhibited cell proliferation and colony formation, with downregulation of cyclin E protein. In conclusion, Rsf-1 is overexpressed in HCCs and serves as a novel tumor marker. Rsf-1 contributes to hepatocellular carcinoma cell growth through regulation of cell cycle proteins.
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Bei'er Road 92, Heping District, Shenyang, Liaoning Province, People's Republic of China.
authors.forename: Chengyao
authors.initials: C
authors.lastname: Xie
authors.forename: Lin
authors.initials: L
authors.lastname: Fu
authors.forename: Lingling
authors.initials: L
authors.lastname: Xie
authors.forename: Nan
authors.initials: N
authors.lastname: Liu
authors.forename: Qingchang
authors.initials: Q
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D006528:Carcinoma, Hepatocellular / Q000401:mortality* / Q000473:pathology; D045744:Cell Line, Tumor; D042002:Chromatin Assembly and Disassembly; D019938:Cyclin D1 / Q000235:genetics; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000401:mortality* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis / Q000502:physiology*; D011379:Prognosis; D015534:Trans-Activators / Q000032:analysis / Q000502:physiology*
chemical_list: D014408:Biomarkers, Tumor; C530104:CCND1 protein, human; D009687:Nuclear Proteins; C452851:RSF1 protein, human; D015534:Trans-Activators; D019938:Cyclin D1