title: Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.
abstract: Identification of the proteinaceous components of the pathological inclusions is an important step in understanding the associated disease mechanisms. We immunohistochemically examined two previously reported cases with eosinophilic neuronal cytoplasmic inclusions (NCIs)(case 1, Mori et al. Neuropathology 2010; 30: 648–53; case 2, Kojima et al. Acta Pathol Jpn 1990; 40: 785–91) using 67 antibodies against proteins related to cytoskeletal constituents, ubiquitin-proteasome system, autophagy-lysosome pathway and stress granule formation. Regional distribution pattern of eosinophilic NCIs in case 1 was substantially different from that in case 2. However, NCIs in both cases were immunonegative for ubiquitin and p62 and were immunopositive for stress granule markers as well as autophagy-related proteins, including valosin-containing protein. Considering that eukaryotic stress granules are cleared by autophagy and valosin-containing protein function, our findings suggest that eosinophilic NCIs in the present two cases may represent the process of autophagic clearance of stress granules.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Mori
authors.forename: Yuka
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Miki
authors.forename: Kunikazu
authors.initials: K
authors.lastname: Tanji
authors.forename: Saori
authors.initials: S
authors.lastname: Odagiri
authors.forename: Komyo
authors.initials: K
authors.lastname: Eto
authors.forename: Koichi
authors.initials: K
authors.lastname: Wakabayashi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001343:Autophagy / Q000502:physiology*; D001344:Autopsy; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D016271:Proto-Oncogene Proteins c-myc; D025801:Ubiquitin; C046951:valosin