title: [Relationship between PMI and relative expression of myocardial various RNAs in rats died of different causes].
abstract: OBJECTIVE
  To observe the changes of relative expression of myocardial various RNAs in rats died of different causes and their relationship with PMI.
  
  
  METHODS
  The rat models were established in which the rats were sacrificed by broken neck, asphyxia, and hemorrhagic shock. Total RNAs were extracted from myocardium. The quantitative real time PCR was used to calculate threshold cycle values of RNAs including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-actin, inducible nitric oxide synthase (iNOS), hypoxia-inducible factor-1 (HIF-1), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and U6 small nuclear RNA (U6 snRNA) and to study the changes of the relative expressions of various indexes with PMI.
  
  
  RESULTS
  U6 snRNA with stable expression level could be used as appropriate internal control. In the early PMI, the relative expression of GAPDH, HIF-1, iNOS, TNF-alpha, and IL-6 more characteristically increased in groups of asphyxia and hemorrhagic shock than in group of broken neck, but the quantity of beta-actin decreased in all groups. In the late PMI, all the relative expressions significantly declined in correlation with the degradation of RNA.
  
  
  CONCLUSIONS
  The characteristic changes of each RNA expression can be used as references to estimate PMI in deaths by different causes.
authors.forename: Ye-Hui
authors.initials: YH
authors.lastname: Lü
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Pan
authors.forename: Kai-Jun
authors.initials: KJ
authors.lastname: Ma
authors.forename: Wen-Can
authors.initials: WC
authors.lastname: Li
authors.forename: Wen-Feng
authors.initials: WF
authors.lastname: Chen
authors.forename: Jie-Qing'
authors.initials: JQ
authors.lastname: Jiang
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Xue
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Wang
authors.forename: Long
authors.initials: L
authors.lastname: Chen
mesh_terms: D000199:Actins; D000818:Animals; D002423:Cause of Death; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D004798:Enzymes / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D009206:Myocardium / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D012313:RNA / Q000378:metabolism*; D012342:RNA, Small Nuclear; D051381:Rats; D012771:Shock, Hemorrhagic; D014409:Tumor Necrosis Factor-alpha
chemical_list: D000199:Actins; D016207:Cytokines; D004798:Enzymes; D012342:RNA, Small Nuclear; D014409:Tumor Necrosis Factor-alpha; C405486:U6 small nuclear RNA; D012313:RNA; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases