title: Effects of chicken-liver hydrolysates on lipid metabolism in a high-fat diet.
abstract: The contents of free hydrophobic amino acids, taurine and carnosine/anserine were elevated after hydrolyzing chicken livers by pepsin and compared to dried chicken livers. Chicken-liver-hydrolysates (CLHs) exhibited in vitro inhibitory lipase activity and bile-acid binding ability (p<0.05). Forty-eight male hamsters were assigned randomly to the following groups: (1) chow diet; (2) high-fat diet (HFD); (3) HFD and 100 mg CLH/kg BW; (4) HFD and 200 mg CLH/kg BW; (5) HFD and 400 mg CLH/kg BW; (6) HFD and 200 mg carnosine/kg BW. CLHs alleviated (p<0.05) serum oxidative stress and improved (p<0.05) the serum lipid profile in the high-fat dietary groups; meanwhile, improved (p<0.05) antioxidant abilities and decreased (p<0.05) lipid accumulation, oxidative stress and TNF-α/IL-1β levels in the livers. These benefits might result from regulations of lipid homeostasis and increased faecal bile-acid outputs (p<0.05). Hence, lipid-homeostasis and antioxidant abilities of CLHs in the high-fat dietary habit were demonstrated and were similar to pure carnosine.
authors.affiliation: Department of Animal Science and Technology, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Kou-Tai
authors.initials: KT
authors.lastname: Yang
authors.affiliation: Department of Animal Science and Technology, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Chen
authors.initials: C
authors.lastname: Lin
authors.affiliation: Department of Post-Modern Agriculture, Ming-Dao University, ChangHua 523, Taiwan.
authors.forename: Cheng-Wei
authors.initials: CW
authors.lastname: Liu
authors.affiliation: Department of Animal Science and Technology, National Taiwan University, Taipei 106, Taiwan. Electronic address: ycpchen@ntu.edu.tw.
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Chen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D002336:Carnosine / Q000008:administration & dosage; D002645:Chickens; D006224:Cricetinae; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects; D004044:Dietary Proteins / Q000008:administration & dosage*; D005234:Fatty Liver / Q000517:prevention & control; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010434:Pepsin A / Q000378:metabolism; D011492:Protein Hydrolysates / Q000008:administration & dosage*; D013654:Taurine / Q000008:administration & dosage; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D000975:Antioxidants; D004041:Dietary Fats; D004044:Dietary Proteins; D008055:Lipids; D011492:Protein Hydrolysates; D014409:Tumor Necrosis Factor-alpha; D013654:Taurine; D002336:Carnosine; D010434:Pepsin A
keywords: Antioxidant capacity; Bile-acid binding ability; Carnosine/anserine; Chicken-liver hydrolysates; Free amino-acid composition; Lipid homeostasis; Pancreatic lipase inhibitory activity