title: Paenibacillus quercus sp. nov., isolated from rhizosphere of Quercus aliena var. acuteserrata.
abstract: A Gram-positive, spore-forming, rod-shaped and motile bacterium, designated strain 1-25(T), was isolated from the rhizosphere of Quercus aliena var. acuteserrata in Taibai Mountain, Shaanxi Province, China. 16S rRNA gene sequence analysis showed that strain 1-25(T) belongs to the genus Paenibacillus. Strain 1-25(T) was found to be closely related to Paenibacillus harenae and Paenibacillus castaneae with 96.0 and 95.9 % 16S rRNA gene sequence similarities, respectively. The strain was observed to grow optimally at 28 °C and pH 7.5. The major isoprenoid quinone was found to be menaquinone-7. The dominant cellular fatty acids were identified as anteiso-C15:0 and iso-C15:0. The diagnostic diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid. The DNA G+C content was determined to be 41.6 mol%. On the basis of phenotypic characteristics and molecular properties, strain 1-25(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus quercus sp. nov. is proposed. The type strain is 1-25(T) (=CCTCC AB2013265(T) = KCTC 33194(T)).
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: Ying-ying
authors.initials: YY
authors.lastname: Jiang
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Wei
authors.forename: Hang-xian
authors.initials: HX
authors.lastname: Lai
authors.forename: Quan-hong
authors.initials: QH
authors.lastname: Xue
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D029963:Quercus / Q000254:growth & development; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid