title: A ten-microRNA signature identified from a genome-wide microRNA expression profiling in human epithelial ovarian cancer.
abstract: Epithelial ovarian cancer (EOC) is the most common gynecologic malignancy. To identify the micro-ribonucleic acids (miRNAs) expression profile in EOC tissues that may serve as a novel diagnostic biomarker for EOC detection, the expression of 1722 miRNAs from 15 normal ovarian tissue samples and 48 ovarian cancer samples was profiled by using a quantitative real-time polymerase chain reaction (qRT-PCR) assay. A ten-microRNA signature (hsa-miR-1271-5p, hsa-miR-574-3p, hsa-miR-182-5p, hsa-miR-183-5p, hsa-miR-96-5p, hsa-miR-15b-5p, hsa-miR-182-3p, hsa-miR-141-5p, hsa-miR-130b-5p, and hsa-miR-135b-3p) was identified to be able to distinguish human ovarian cancer tissues from normal tissues with 97% sensitivity and 92% specificity. Two miRNA clusters of miR183-96-183 (miR-96-5p, and miR-182, miR183) and miR200 (miR-141-5p, miR200a, b, c and miR429) are significantly up-regulated in ovarian cancer tissue samples compared to those of normal tissue samples, suggesting theses miRNAs may be involved in ovarian cancer development.
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Biovue Technology (China) Ltd., Shanghai, China.
authors.forename: Miao-Jun
authors.initials: MJ
authors.lastname: Zhu
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Ren
authors.affiliation: Biovue Technology (China) Ltd., Shanghai, China.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Wu
authors.affiliation: Biovue Technology (China) Ltd., Shanghai, China.
authors.forename: Wu-Mei
authors.initials: WM
authors.lastname: Han
authors.affiliation: Biovue Technology (China) Ltd., Shanghai, China.
authors.forename: Ruo-Ying
authors.initials: RY
authors.lastname: Tan
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Rui-Qin
authors.initials: RQ
authors.lastname: Tu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D055106:Genome-Wide Association Study / Q000379:methods; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000235:genetics*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C533895:MIRN141 microRNA, human; C533449:MIRN183 microRNA, human; C533896:MIRN200 microRNA, human; C546363:MIRN429 microRNA, human; C541492:MIRN96 microRNA, human; D035683:MicroRNAs; C538984:Mirn182 microRNA, human