title: Simultaneous photodynamic and photothermal therapy using photosensitizer-functionalized Pd nanosheets by single continuous wave laser.
abstract: In this work, we prepared chlorin e6 (Ce6)-functionalized Pd nanosheets (Pd-PEI-Ce6) for the photodynamic and photothermal combined therapy that use a single laser. To fabricate the Pd-PEI-Ce6 nanocomposite, photosensitizer Ce6 were chemically conjugated to polyethylenimine (PEI) and the formed Ce6-PEI conjugates were then anchored onto Pd nanosheets by electrostatic and coordination interaction. The prepared Pd-PEI-Ce6 nanocomposite were about 4.5 nm in size, exhibited broad, and strong absorption from 450 to 800 nm, good singlet oxygen generation capacity and photothermal conversion efficiency, and excellent biocompability. Significantly greater cell killing was observed when HeLa cells incubated with Pd-PEI-Ce6 were irradiated with the 660 nm laser, attributable to both Pd nanosheets-mediated photothermal ablation and the photodynamic destruction effect of photosensitizer Ce6. The double phototherapy effect was also confirmed in vivo. It was found that the Pd-PEI-Ce6 treated tumor-bearing mice displayed the enhanced therapeutic efficiency compared to that of Pd-PEI, or Ce6-treated mice. Our work highlights the promise of using Pd nanosheets for potential multimode cancer therapies.
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University , Xiamen, 361005, P.R. China.
authors.forename: Zengxia
authors.initials: Z
authors.lastname: Zhao
authors.forename: Saige
authors.initials: S
authors.lastname: Shi
authors.forename: Yizhuan
authors.initials: Y
authors.lastname: Huang
authors.forename: Shaoheng
authors.initials: S
authors.lastname: Tang
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*; D017319:Photosensitizing Agents / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D010165:Palladium