title: [Production of sugar syrup containing rare sugar using dual-enzyme coupled reaction system].
abstract: Enzymatic conversion is very important to produce functional rare sugars, but the conversion rate of single enzymes is generally low. To increase the conversion rate, a dual-enzyme coupled reaction system was developed. Dual-enzyme coupled reaction system was constructed using D-psicose-3-epimerase (DPE) and L-rhamnose isomerase (L-RhI), and used to convert D-fructose to D-psicose and D-allose. The ratio of DPE and L-RhI was 1:10 (W/W), and the concentration of DPE was 0.05 mg/mL. The optimum temperature was 60 degrees C and pH was 9.0. When the concentration of D-fructose was 2%, the reaction reached its equilibrium after 10 h, and the yield of D-psicose and D-allose was 5.12 and 2.04 g/L, respectively. Using the dual-enzymes coupled system developed in the current study, we could obtain sugar syrup containing functional rare sugar from fructose-rich raw material, such as high fructose corn syrup.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Han
authors.forename: Yueming
authors.initials: Y
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Bai
authors.forename: Ken
authors.initials: K
authors.lastname: Izumori
authors.forename: Tongcun
authors.initials: T
authors.lastname: Zhang
authors.forename: Yuanxia
authors.initials: Y
authors.lastname: Sun
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000378:metabolism*; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D005632:Fructose / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
chemical_list: C003243:psicose; D005632:Fructose; C002055:allose; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C062878:L-rhamnose isomerase; D005947:Glucose