title: Induction of quinone reductase (QR) by withanolides isolated from Physalis angulata L. var. villosa Bonati (Solanaceae).
abstract: In the present study, the EtOAc extract of the persistent calyx of Physalis angulata L. var. villosa Bonati (PA) was tested for its potential quinone reductase (QR) inducing activity with glutathione (GSH) as the substrate using an UPLC-ESI-MS method. The result revealed that the PA had electrophiles that could induce quinone reductase (QR) activity, which might be attributed to the modification of the highly reactive cysteine residues in Keap1. Herein, three new withanolides, compounds 3, 6 and 7, together with four known withanolides, compounds 1, 2, 4 and 5 were isolated from PA extract. Their structures were determined by spectroscopic techniques, including (1)H-, (13)C NMR (DEPT), and 2D-NMR (HMBC, HMQC, (1)H, (1)H-COSY, NOESY) experiments, as well as by HR-MS. All the seven compounds were tested for their QR induction activities towards mouse hepa 1c1c7 cells.
authors.affiliation: Institute of Marine Biology & Natural Products, Ocean College, Zhejiang University, Zijingang Campus, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.affiliation: Institute of Marine Biology & Natural Products, Ocean College, Zhejiang University, Zijingang Campus, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Institute of Marine Biology & Natural Products, Ocean College, Zhejiang University, Zijingang Campus, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Yu
authors.affiliation: Institute of Marine Biology & Natural Products, Ocean College, Zhejiang University, Zijingang Campus, No. 866 Yuhangtang Road, Hangzhou 310058, PR China. Electronic address: mazj@zju.edu.cn.
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Zhejiang Key Laboratory of Gastro-Intestinal Pathophysiology, Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, No. 54 Youdian Road, Hangzhou 310006, PR China.
authors.forename: Xiaoqiong
authors.initials: X
authors.lastname: Ma
authors.affiliation: Zhejiang Key Laboratory of Gastro-Intestinal Pathophysiology, Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, No. 54 Youdian Road, Hangzhou 310006, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Zhejiang Institute for Drug Control, No. 86 Jichang Road, Hangzhou 310004, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Zhejiang Provincial People's Hospital, No. 158 Shangtang Rd., Hangzhou 310014, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004789:Enzyme Activation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D051379:Mice; D008968:Molecular Conformation; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism*; D019657:Solanaceae / Q000737:chemistry*; D012995:Solubility; D021241:Spectrometry, Mass, Electrospray Ionization; D014407:Tumor Cells, Cultured; D054358:Withanolides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D054358:Withanolides; D016660:NAD(P)H Dehydrogenase (Quinone); D005978:Glutathione
keywords: Physalis angulata L. var. villosa Bonati; QR induction activity; UPLC–ESI-MS analysis; Withanolides