title: [A preliminary study on genome-wide expression profiling of laryngeal squamous cell carcinoma].
abstract: OBJECTIVE
  To identify the genes differentially expressed and underlying molecular mechanism in laryngeal squamous cell carcinoma by using cDNA microarrays.
  
  
  METHODS
  Using Illumina Human HT-12 BeadChip, gene expressions were detected in ten pairs of laryngeal cancer tissues and adjacent normal tissues. Total RNA was extracted and reverse-transcribed into cDNA. Labeled cDNA were hybridized with cDNA microarray, data were read and images were scanned. All the samples had passed quality control testing.
  
  
  RESULTS
  Through Illumina Genomstudio 1.9.0 Data processing software (P < 0.05 or diffscore>13, diffscore<-13) and multiple displacement t test(FDR<0.05), 426 genes showed statistically significant differences in expressions between laryngeal tumor tissues and corresponding adjacent normal tissues, with 222 up-regulated genes and 204 down-regulated genes in laryngeal cancer tissues. These up- or down-regulated genes were indicated to involve in cellular processes relevant to the cancer phenotype, such as proliferation, cell cycle, chromosome segregation, mitosis and meiosis. These differentially expressed genes also took part in cancer related signaling pathways as well, for instance, metabolic pathways, cell cycle, DNA replication, glutathione metabolism, mucin type O-Glycan biosynthesis, drug metabolism-cytochrome P450 and so on.
  
  
  CONCLUSIONS
  The set of genes identified here and their functional annotations contribute to a better understanding of the pathogenesis of laryngeal squamous cell carcinoma from the view of multiple gene interactions and provide candidate markers for improving diagnosis, prognosis and treatment of this cancer.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100730, hina.
authors.forename: Ru
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100730, hina.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100730, hina.
authors.forename: Meng
authors.initials: M
authors.lastname: Lian
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100730, hina.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100730, hina.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100730, hina.
authors.forename: Ling
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100730, hina. Email: fangjugao@vip.sohu.com.
authors.forename: Jugao
authors.initials: J
authors.lastname: Fang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055106:Genome-Wide Association Study; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D000077195:Squamous Cell Carcinoma of Head and Neck