title: Split luciferase complementation for analysis of intracellular signaling.
abstract: Bioluminescent proteins such as luciferases are unique analytical tools with high sensitivity and wide dynamic ranges. However, applicability of the proteins has remained limited to reporter gene analysis. Split luciferase complementation technique is a new powerful strategy to detect protein-protein interactions and their related intracellular signaling in living cells. This review presents a summary of recent topics for the analysis of biological events using split luciferase complementation techniques. Different applications such as G-protein-coupled receptor (GPCR) screening, monitoring phosphorylations, protein-protein interactions, and pH differences in living tissues are described.
authors.affiliation: Department of Chemistry, School of Science, The University of Tokyo.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Hattori
authors.forename: Takeaki
authors.initials: T
authors.lastname: Ozawa
mesh_terms: D000818:Animals; D002460:Cell Line; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; D008156:Luciferases