title: [Analysis of Pfcrt gene polymorphism in Plasmodium falciparum from imported cases].
abstract: OBJECTIVE
  To identify the incidence of the K76T mutation in Pfcrt gene of imported Plasmodium falciparum and study the Pfcrt gene polymorphism in Plasmodium falciparum.
  
  
  METHODS
  Seventy-two blood samples were collected from patients infected with P. falciparum returning from Africa (Nigeria, Equatorial Guinea, Congo, Liberia, Angora and Mali) and Southeast Asia (Myanmar and Indonesia) from 2008 to 2012. According to Pfcrt gene sequence of P. falciparum, nested PCR primers were designed, and the reaction was applied with P. falciparum DNA in the blood samples as templates. PCR products were identified by Apo I digestion.
  
  
  RESULTS
  Among 72 blood samples of P. falciparum, mutant Pfcrt alleles were found in 41 samples (57.7%, 41/71) and wild type Pfcrt alleles were found in 30 samples (42.3%, 30/71). There were 25 samples (50%, 25/50) each with mutant Pfcrt alleles or wild type that were from Africa, while 16 samples (76.2%, 16/21) with mutant Pfcrt alleles and 5 samples (23.8%, 5/21) with wild type that were from Southeast Asia, respectively.
  
  
  CONCLUSIONS
  The incidence of Pfcrt gene mutation is different in P. falciparum isolates from different regions.
authors.forename: Shui-Mao
authors.initials: SM
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Kai
authors.initials: K
authors.lastname: Wu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Ming-Xing
authors.initials: MX
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Chong-Xin
authors.initials: CX
authors.lastname: Wang
mesh_terms: D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology; D026901:Membrane Transport Proteins / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics* / Q000302:isolation & purification; D017354:Point Mutation; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan; D026901:Membrane Transport Proteins; C416369:PfCRT protein, Plasmodium falciparum; D015800:Protozoan Proteins