title: Nicotine promotes late endothelial progenitor cells functional activity in a PI 3-kinase-dependent manner.
abstract: Recent studies have shown that endothelial progenitor cells (EPCs) participated in angiogenic effects of nicotine and nicotine dose dependently increased the functional activity of early EPCs. The effects of nicotine on late EPCs remain to be determined. Therefore, we investigated whether nicotine had influences on the functional activity of late EPCs. Late EPCs were isolated from human umbilical cord blood and characterized. Late EPCs of 3-5 passages were treated for 32 h with either vehicle or nicotine. The proliferative, migratory, and in vitro vasculogenesis activities of late EPCs were assayed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, modified Boyden chamber assay, and in matrigel, respectively. Late EPCs adhesion assay was performed by replating cells on fibronectin-coated dishes, and then adherent cells were counted. Nicotine enhanced proliferative, migratory, adhesive, and in vitro vasculogenesis capacities of late EPCs. These effects were significantly reduced in the presence of phosphatidylinositol (PI) 3-kinase inhibitor.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Shantou University Medical College, Shantou, 515041, Guangdong, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.forename: Zhouwu
authors.initials: Z
authors.lastname: Shu
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Xuerui
authors.initials: X
authors.lastname: Tan
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D066026:Endothelial Progenitor Cells / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009538:Nicotine / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D013997:Time Factors
chemical_list: D009538:Nicotine; D019869:Phosphatidylinositol 3-Kinases