title: Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography.
abstract: Cryo-electron tomography (CET) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated cryo-PALM (photoactivated localization microscopy)-CET method for localizing objects within cryo-tomograms to beyond the diffraction limit of the light microscope. Using cryo-PALM-CET, we identified multiple and new conformations of the dynamic type VI secretion system in the crowded interior of Myxococcus xanthus.
authors.affiliation: 1] Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA. [2] Howard Hughes Medical Institute, Pasadena, California, USA.
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Chang
authors.affiliation: 1] Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA. [2] Howard Hughes Medical Institute, Pasadena, California, USA.
authors.forename: Songye
authors.initials: S
authors.lastname: Chen
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
authors.forename: Elitza I
authors.initials: EI
authors.lastname: Tocheva
authors.affiliation: Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Treuner-Lange
authors.affiliation: Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Löbach
authors.affiliation: Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
authors.forename: Lotte
authors.initials: L
authors.lastname: Søgaard-Andersen
authors.affiliation: 1] Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA. [2] Howard Hughes Medical Institute, Pasadena, California, USA.
authors.forename: Grant J
authors.initials: GJ
authors.lastname: Jensen
mesh_terms: D058947:Bacterial Secretion Systems; D020285:Cryoelectron Microscopy / Q000379:methods*; D055032:Electron Microscope Tomography / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods; D016941:Myxococcus xanthus / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D058947:Bacterial Secretion Systems