title: Complete nucleotide sequence and genome organization of an endornavirus from bottle gourd (Lagenaria siceraria) in California, U.S.A.
abstract: The full-length nucleotide sequence and genome organization of an Endornavirus isolated from ornamental hard shell bottle gourd plants (Lagenaria siceraria (Molina) Standl.) in California (CA), USA tentatively named L. siceraria endornavirus-California (LsEV-CA) was determined. The LsEV-CA genome was 15088 bp in length, with a G + C content of 36.55 %. The lengths of the 5' and 3' untranslated regions were 111 and 52 bp, respectively. The genome of LsEV-CA contained one large ORF encoding a 576 kDa polyprotein. The predicted protein contains two glycosyltransferase motifs, as well as RNA-dependent RNA polymerase and helicase domains. LsEV-CA was detected in healthy-looking field-grown gourd plants, as well as plants expressing yellows symptoms. It was also detected in non-symptomatic greenhouse-grown gourd seedlings grown from seed obtained from the same field sites. These preliminary data indicate that LsEV-CA is likely not associated with the gourd-yellows syndrome observed in the field.
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, CA, 92521, USA.
authors.forename: Sun-Jung
authors.initials: SJ
authors.lastname: Kwon
authors.forename: Shih-Hua
authors.initials: SH
authors.lastname: Tan
authors.forename: Georgios
authors.initials: G
authors.lastname: Vidalakis
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D001482:Base Composition; D002140:California; D019662:Cucurbitaceae / Q000821:virology*; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010942:Plant Viruses / Q000235:genetics / Q000302:isolation & purification; D020815:Polyproteins / Q000737:chemistry / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D020815:Polyproteins; D012367:RNA, Viral