title: Genome-wide association study identifies quantitative trait loci affecting hematological traits in an F2 intercross between Landrace and Korean native pigs.
abstract: Changes affecting the status of health and robustness can bring about physiological alterations including hematological parameters in swine. To identify quantitative trait loci (QTL) associated with eight hematological traits (one leukocyte trait, six erythrocyte traits and one platelet trait), we conducted a genome-wide association study using the PorcineSNP60K BeadChip in a resource population derived from an intercross between Landrace and Korean native pigs. A total of 36 740 SNPs from 816 F2 progeny were analyzed for each blood-related trait after filtering for quality control. Data were analyzed by the genome-wide rapid association using mixed model and regression (GRAMMAR) approach. A total of 257 significant SNPs (P < 1.36 × 10(-6) ) on SSC3, 6, 8, 13 and 17 were identified for blood-related traits in this study. Interestingly, the genomic region between 17.9 and 130 Mb on SSC8 was found to be significantly associated with red blood cell, mean corpuscular volume and mean corpuscular hemoglobin. Our results include the identification of five significant SNPs within five candidate genes (KIT, IL15, TXK, ARAP2 and ERG) for hematopoiesis. Further validation of these identified SNPs could give valuable information for understanding the variation of hematological traits in pigs.
authors.affiliation: Department of Animal Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju, 660-701, Korea; Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju, 660-701, Korea.
authors.forename: E J
authors.initials: EJ
authors.lastname: Jung
authors.forename: H B
authors.initials: HB
authors.lastname: Park
authors.forename: J B
authors.initials: JB
authors.lastname: Lee
authors.forename: C K
authors.initials: CK
authors.lastname: Yoo
authors.forename: B M
authors.initials: BM
authors.lastname: Kim
authors.forename: H I
authors.initials: HI
authors.lastname: Kim
authors.forename: I C
authors.initials: IC
authors.lastname: Cho
authors.forename: H T
authors.initials: HT
authors.lastname: Lim
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology; D003433:Crosses, Genetic; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D055106:Genome-Wide Association Study; D007962:Leukocytes / Q000166:cytology; D008297:Male; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D034421:Sus scrofa / Q000097:blood* / Q000235:genetics*
keywords: blood-related traits; health; pig; robustness; whole-genome association study