title: NtrBC and Nac contribute to efficient Shigella flexneri intracellular replication.
abstract: Shigella flexneri two-component regulatory systems (TCRS) are responsible for sensing changes in environmental conditions and regulating gene expression accordingly. We examined 12 TCRS that were previously uncharacterized for potential roles in S. flexneri growth within the eukaryotic intracellular environment. We demonstrate that the TCRS EvgSA, NtrBC, and RstBA systems are required for wild-type plaque formation in cultured epithelial cells. The phenotype of the NtrBC mutant depended in part on the Nac transcriptional regulator. Microarray analysis was performed to identify S. flexneri genes differentially regulated by the NtrBC system or Nac in the intracellular environment. This study contributes to our understanding of the transcriptional regulation necessary for Shigella to effectively adapt to the mammalian host cell.
authors.affiliation: Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, Oregon, USA.
authors.forename: Chelsea D
authors.initials: CD
authors.lastname: Waddell
authors.affiliation: Department of Biology, University of Richmond, Richmond, Virginia, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Walter
authors.affiliation: Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, Oregon, USA.
authors.forename: Sophia A
authors.initials: SA
authors.lastname: Pacheco
authors.affiliation: Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, Oregon, USA purdyg@ohsu.edu lrunyenj@richmond.edu.
authors.forename: Georgiana E
authors.initials: GE
authors.lastname: Purdy
authors.affiliation: Department of Biology, University of Richmond, Richmond, Virginia, USA purdyg@ohsu.edu lrunyenj@richmond.edu.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Runyen-Janecky
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000382:microbiology; D040081:Protein Array Analysis; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D050436:Regulatory Elements, Transcriptional / Q000235:genetics; D012763:Shigella flexneri / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial