title: Effects of ubiquitin-conjugating enzyme 2C on invasion, proliferation and cell cycling of lung cancer cells.
abstract: The aims of this study were to investigate the influence of ubiquitin- conjugating enzyme E2C (UBE2C) on biological behavior of lung cancer cells. Using MTT, flow cytometry and invasion assays, we detected UBE2C expression and evaluated its biological properties in these cells, including effects on proliferation, the cell cycle profile and invasive capability. Compared with control cells, the UBE2C transfected cells demonstrated increased cellular proliferation (p<0.05). UBE2C transfected cells also had a lower percentage in G1 phase and a higher percentage in S phase (p<0.05). Importantly, the UBE2C transfected cells had a notable enhancement of cell numbers penetrating the basement membrane compared with the control group (p<0.05). Ectopic up- regulation UBE2C promoted the growth of lung cancer cells in vivo. Furthermore, we found UBE2C increased the expression of cyclin D1 and MMP-2. These results show UBE2C may represent a potential therapeutic target for lung cancer.
authors.affiliation: Department of Respiratory Disease, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China E-mail : dlichen@126.com.
authors.forename: Xiao-Kui
authors.initials: XK
authors.lastname: Tang
authors.forename: Ke-Jian
authors.initials: KJ
authors.lastname: Wang
authors.forename: Yu-Kui
authors.initials: YK
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Chen
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D019938:Cyclin D1 / Q000096:biosynthesis; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D009361:Neoplasm Invasiveness / Q000235:genetics*; D016196:S Phase / Q000235:genetics*; D044763:Ubiquitin-Conjugating Enzymes / Q000096:biosynthesis / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D019938:Cyclin D1; C105156:UBE2C protein, human; D044763:Ubiquitin-Conjugating Enzymes; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9