title: Injury to the endothelial surface layer induces glomerular hyperfiltration rats with early-stage diabetes.
abstract: Glomerular endothelial surface layer (ESL) may play a role in the mechanisms of albuminuria in diabetic nephropathy, which lack evidence in vivo. The effects of high glucose on the passage of albumin across the glomerular ESL were analysed in streptozotocin-induced diabetic Sprague-Dawley rats for 4 weeks. Albuminuria and glomerular mesangial matrix were significantly increased in diabetic rats. The passage of albumin across the ESL, as measured by albumin-colloid gold particle density in the glomerular basement membrane (GBM), was increased significantly in diabetic rats. The thickness of the glomerular ESL, examined indirectly by infusing Intralipid into vessels using an electron microscope, was significantly decreased and the GBM exhibited little change in diabetic rats. In summary, the glomerular ESL may play a role in the pathogenesis of albuminuria in rats with early-stage diabetes.
authors.affiliation: Division of Endocrinology, Shanghai Tongji Hospital Affiliated Tongji University, No. 389, Xincun Road, Shanghai 200065, China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Division of Endocrinology, Shanghai Tongji Hospital Affiliated Tongji University, No. 389, Xincun Road, Shanghai 200065, China ; Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Division of Endocrinology, Shanghai Tongji Hospital Affiliated Tongji University, No. 389, Xincun Road, Shanghai 200065, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Division of Endocrinology, Shanghai Tongji Hospital Affiliated Tongji University, No. 389, Xincun Road, Shanghai 200065, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Xuan
authors.affiliation: Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai 200041, China.
authors.forename: Lanyu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Deng
authors.affiliation: Division of Endocrinology, Shanghai Tongji Hospital Affiliated Tongji University, No. 389, Xincun Road, Shanghai 200065, China.
authors.forename: Keqin
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai 200041, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Division of Endocrinology, Shanghai Tongji Hospital Affiliated Tongji University, No. 389, Xincun Road, Shanghai 200065, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Lei
mesh_terms: D000419:Albuminuria / Q000209:etiology; D000818:Animals; D002199:Capillary Permeability; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D003928:Diabetic Nephropathies / Q000097:blood / Q000473:pathology / Q000503:physiopathology* / Q000652:urine; D004655:Emulsions / Q000008:administration & dosage; D004730:Endothelium, Vascular / Q000378:metabolism / Q000503:physiopathology* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D050533:Glomerular Basement Membrane / Q000378:metabolism / Q000503:physiopathology / Q000648:ultrastructure; D060730:Glomerular Filtration Barrier / Q000378:metabolism / Q000503:physiopathology* / Q000648:ultrastructure; D005920:Glomerular Mesangium / Q000378:metabolism / Q000648:ultrastructure; D017979:Gold Colloid; D007275:Injections, Intravenous; D007678:Kidney Glomerulus / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology* / Q000648:ultrastructure; D008297:Male; D010743:Phospholipids / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin; D013024:Soybean Oil / Q000008:administration & dosage; D013311:Streptozocin; D014682:Vena Cava, Inferior
chemical_list: D004655:Emulsions; D017979:Gold Colloid; D010743:Phospholipids; D012709:Serum Albumin; C545823:soybean oil, phospholipid emulsion; D013311:Streptozocin; D013024:Soybean Oil