title: Isolation and substrate specificity of an adenine nucleoside phosphorylase from adult Schistosoma mansoni.
abstract: An adenine nucleoside phosphorylase (ANP, EC none) activity was identified and partially purified from extracts of Schistosoma mansoni by chromatofocussing column chromatography and molecular sieving. The enzyme is distinct from purine nucleoside phosphorylase (PNP, EC 2.4.2.1). ANP is specific for adenine nucleosides which includes adenosine analogs modified in the aglycone, pentose or both moieties. (e.g. 2'-deoxyadenosine, 5'-deoxy-5'-methylthioadenosine, 5'-deoxy-5'-iodo-2-fluoroadenosine, etc.) The enzyme is also distinct from the mammalian 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP, EC 2.4.2.28) in that it is able of the phosphorolysis of 2'-deoxyadenosine while mammalian MTAP cannot. Because of ANP unique substrate specificity, the enzyme could play a role as a target for chemotherapy of these parasites. Cytotoxic analogs may be designed as subversive substrates that are selectively activated only by the schistosomal ANP.
authors.affiliation: Division of Biology and Medicine, Brown University, RI 02912, USA.
authors.forename: Todd M
authors.initials: TM
authors.lastname: Savarese
authors.affiliation: Division of Biology and Medicine, Brown University, RI 02912, USA; Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA. Electronic address: melkouni@uab.edu.
authors.forename: Mahmoud H
authors.initials: MH
authors.lastname: El Kouni
mesh_terms: D000225:Adenine / Q000378:metabolism*; D000818:Animals; D010430:Pentosyltransferases / Q000302:isolation & purification* / Q000378:metabolism*; D012550:Schistosoma mansoni / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D010430:Pentosyltransferases; C067995:nucleoside phosphorylase; D000225:Adenine
keywords: Adenine nucleoside phosphorylase; Adenosine metabolism; Purine nucleoside phosphorylase (EC 2.4.2.1) 5′-deoxy-5′-methylthioadenosine phosphorylase (EC 2.4.2.28); Schistosoma mansoni