title: Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays.
abstract: In Pseudomonas aeruginosa, identification of new partners of a protein of interest could give precious clues to decipher a biological process in which this protein is involved. However, genes encoding for partners of a protein of interest are unknown and frequently scattered throughout the genome. We describe herein the construction and the use of pan-genomic bacterial two-hybrid libraries to identify new partners of a protein of interest encoded by P. aeruginosa.
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), UMR7255 CNRS-Aix Marseille Université, Institut de Microbiologie de la Méditerranée, Marseille cédex 20, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: de Bentzmann
authors.forename: Christophe
authors.initials: C
authors.lastname: Bordi
mesh_terms: D000242:Cyclic AMP / Q000096:biosynthesis; D017930:Genes, Reporter; D016680:Genome, Bacterial; D057166:High-Throughput Screening Assays / Q000379:methods*; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D020798:Two-Hybrid System Techniques
chemical_list: D000242:Cyclic AMP