title: The influence of apolipoprotein A5 T-1131C and apolipoprotein E common genetic variants on the levels of hemostatic markers in dyslipidemic patients.
abstract: OBJECTIVE
  The aim of this study was to evaluate the relationships of the T-1131C (rs662799) polymorphism variants of apolipoprotein A5 (Apo A5) gene and variants of apolipoprotein E (Apo E) gene common polymorphism (rs429358, rs7412) to selected hemostatic markers.
  
  
  METHODS
  We examined 590 asymptomatic dyslipidemic patients, subsequently divided into MetS+ (n=146) and MetS- (n=444) groups according to the criteria for identification of the metabolic syndrome (MetS). We compared variant frequencies and differences in levels of hemostatic markers according to Apo A5, Apo E and Apo A5/Apo E common variants.
  
  
  RESULTS
  The -1131C Apo A5 minor variant was associated with elevated tissue plasminogen activator (tPA) in comparison to TT genotype (p<0.001), but not in the MetS+ group. The analysis of Apo A5/Apo E common variants in all subjects revealed that the presence of -1131C minor allele has always been associated with higher levels of tPA in comparison with T allele, regardless of Apo E genotype. Also the presence of minor Apo E2 allele led to elevated tPA concentrations in both T and C carriers. In addition, common -1131C/E2 variant was associated with the highest tPA levels.
  
  
  CONCLUSIONS
  We demonstrated a remarkable association especially between the -1131C Apo A5 variant and increased tPA levels in asymptomatic dyslipidemic patients.
authors.affiliation: Department of Clinical Biochemistry, University Hospital Olomouc, I.P. Pavlova 6, 775 20 Olomouc, Czech Republic. Electronic address: dalibor.novotny@fnol.cz.
authors.forename: Dalibor
authors.initials: D
authors.lastname: Novotny
authors.affiliation: 3rd Department of Internal Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, I.P. Pavlova 6, 775 20 Olomouc, Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: Karasek
authors.affiliation: 3rd Department of Internal Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, I.P. Pavlova 6, 775 20 Olomouc, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Vaverkova
authors.affiliation: Department of Haemato-Oncology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, I.P. Pavlova 6, 775 20 Olomouc, Czech Republic.
authors.forename: Ludek
authors.initials: L
authors.lastname: Slavik
mesh_terms: D000328:Adult; D001054:Apolipoproteins A / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics*; D058070:Asymptomatic Diseases; D015415:Biomarkers / Q000097:blood; D050171:Dyslipidemias / Q000097:blood* / Q000235:genetics* / Q000503:physiopathology; D005260:Female; D006487:Hemostasis; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D010959:Tissue Plasminogen Activator / Q000097:blood*
chemical_list: D001054:Apolipoproteins A; D001057:Apolipoproteins E; D015415:Biomarkers; D010959:Tissue Plasminogen Activator
keywords: Apolipoprotein A5; Apolipoprotein E; Dyslipidemia; Genetic polymorphism; Metabolic syndrome; Tissue plasminogen activator