title: [Extraction of sperm DNA from mixed stain by the modified differential lysis method combined with silicon bead method].
abstract: OBJECTIVE
  To extract sperm DNA from mixed stain by the modified differential lysis method combined with silicon bead method and to evaluate its application value.
  
  
  METHODS
  Fifty-two mixed stains containing female STR genotypes detected by differential lysis method were collected. The sperm DNA was extracted by the modified method combined with silicon bead method, then genotyped with the Identifiler Kit, and compared with the results of genotyping by the conventional differential lysis method as control.
  
  
  RESULTS
  Of the 52 samples, 38 samples with sole male STR genotypes in all loci were detected. The detection rate of male STR genotypes was 98.08% through the modified method combined with silicon bead method.
  
  
  CONCLUSIONS
  The modified differential lysis method combined with silicon bead method can be used in extraction of sperm DNA from mixed stain.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Han
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Hai
authors.initials: H
authors.lastname: Yi
authors.forename: Geng-Ye
authors.initials: GY
authors.lastname: Yang
authors.forename: Dong-Tao
authors.initials: DT
authors.lastname: Jia
authors.forename: Da-Ru
authors.initials: DR
authors.lastname: Lu
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012825:Silicon; D013094:Spermatozoa
chemical_list: D004247:DNA; D012825:Silicon