Fabrication of carbon-felt-based multi-enzyme immobilized anodes to oxidize sucrose for biofuel cells.
A carbon-felt-based multi-enzyme immobilized bioanode for biofuel cells has been successfully developed. The combination of four enzymes, namely, invertase, mutarotase, glucose oxidase, and fructose dehydrogenase, makes it possible to use sucrose--a disaccharide--as fuel for the bioanode. The new electrode exhibits a high oxidation current density of about 12 mA cm(-2) (0.25 V vs. Ag/AgCl) in a McIlvaine buffer solution containing sucrose at pH 6.0 in the half-cell configuration. A sucrose/O(2) biofuel cell composed of the bioanode and an opposite biocathode, including bilirubin oxidase as the enzymatic electrocatalyst, was fabricated, and the new device demonstrated a maximum power density of 2.90 mW cm(-2) with an open-circuit voltage of 0.69 V in the McIlvaine buffer solution. The biofuel cell fabricated using our multi-enzyme anode operates in commercially available beverages that contain only sugar, even without glucose.
Department of Applied Chemistry, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601 (Japan).
Yutaka
Y
Handa
Kiyofumi
K
Yamagiwa
Yutaro
Y
Ikeda
Yuichi
Y
Yanagisawa
Shinya
S
Watanabe
Naoaki
N
Yabuuchi
Shinichi
S
Komaba
D001674:Bioelectric Energy Sources; D002238:Carbohydrate Epimerases / Q000737:chemistry; D002244:Carbon / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005632:Fructose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D013395:Sucrose / Q000737:chemistry*; D043324:beta-Fructofuranosidase / Q000737:chemistry
D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D005632:Fructose; D013395:Sucrose; D002244:Carbon; D010088:Oxidoreductases; D005949:Glucose Oxidase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D043324:beta-Fructofuranosidase; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase; D005947:Glucose; D010100:Oxygen
beverages; biofuel cells; buffer solutions; multi-enzyme systems; sucrose