A size amplified immune magnetic microbeads strategy in the rapid detection of circulating tumor cells.
Using anti-EpCAM antibody modified magnetic microbeads allowed us to simultaneously apply size-amplification and magnetic labelling of CTCs to the capture and purification of CTCs by membrane filtration and immune-magnetic separation. High purity capture (>98%), rapid (<2 hours) and simple detection of CTCs were realized.
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan, China. tangb@sdnu.edu.cn.
Hongyan
H
Zhang
Yanhong
Y
Wang
Qingling
Q
Li
Fumiao
F
Zhang
Bo
B
Tang
D000912:Antibodies, Neoplasm / Q000737:chemistry* / Q000276:immunology*; D045744:Cell Line, Tumor; D002469:Cell Separation; D005374:Filtration; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D008280:Magnetics; D008567:Membranes, Artificial; D008863:Microspheres; D053758:Nanoparticles; D009360:Neoplastic Cells, Circulating / Q000276:immunology*
D000912:Antibodies, Neoplasm; D008567:Membranes, Artificial