Cytotoxic effect of ergot alkaloids in Achnatherum inebrians infected by the Neotyphodium gansuense endophyte.
Ergonovine or ergonovinine was isolated from the aerial parts of endophyte (Neotyphodium gansuense) infected (E+) drunken horse grass (Achnatherum inebrians), neither of which existed in endophyte-free (E-) plants. Both of these ergot alkaloids had a cytotoxic effect on animal smooth muscle cells and increased cell growth inhibition with greater concentrations, in a significantly (P < 0.05) positive correlation. The median inhibitory concentrations (IC50) for ergonovine and ergonovinine were 71.95 and 72.75 μg/mL, respectively. These results indicate that endophytic ergot alkaloids may be the cause of drunken horse grass poisoning.
State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University , P.O. Box 61, Lanzhou 730020, People's Republic of China.
Xingxu
X
Zhang
Zhibiao
Z
Nan
Chunjie
C
Li
Kun
K
Gao
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D060026:Endophytes / Q000737:chemistry; D004874:Ergonovine / Q000737:chemistry / Q000633:toxicity; D004876:Ergot Alkaloids / Q000737:chemistry / Q000633:toxicity*; D020128:Inhibitory Concentration 50; D032389:Myocytes, Smooth Muscle / Q000187:drug effects; D055328:Neotyphodium / Q000737:chemistry*; D010939:Plant Poisoning / Q000662:veterinary; D006109:Poaceae / Q000382:microbiology*
D004876:Ergot Alkaloids; D004874:Ergonovine