[Defects in TOR regulatory complexes retard aging and carbonyl/oxidative stress development in yeast Saccharomyces cerevisiae].
TOR signaling pathway first described in yeast S. cerevisiae is the highly conserved regulator of eukaryotic cell growth, aging and stress resistance. The effect of nitrogen sources, in particular amino acids, on the activity of TOR signaling pathway is well studied, however its relation to carbohydrates is poor understood. The aim of the present study is expanding of our understanding of potential role of TOR regulatory complexes in development of carbonyl/oxidative stress that can result from yeast cultivation on glucose and fructose. It has been shown that the level of alpha-dicarbonyl compounds and protein carbonyl groups increased with time of yeast cultivation and was higher in cells grown on fructose that demonstrated their accelerated aging and carbonyl/oxidative stress development as compared with cells grown on glucose. The strains defective in TOR proteins cultivated in the presence of glucose as well as fructose demonstrated lower markers of the stress and aging than parental strain. Thus these data confirmed the previous conclusion on fructose more potent ability to cause carbonyl/oxidative stress and accelerated aging in S. cerevisiae as compared with glucose. However, defects in TOR regulatory complexes retard aging and development of the stress in yeast independent on the type of carbohydrate in the cultivation medium.
B V
BV
Homza
R A
RA
Vasyl'kovs'ka
H M
HM
Semchyshyn
D018797:Cell Cycle Proteins / Q000172:deficiency / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D005632:Fructose / Q000378:metabolism / Q000494:pharmacology*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D018384:Oxidative Stress; D019869:Phosphatidylinositol 3-Kinases / Q000172:deficiency / Q000235:genetics*; D050050:Protein Carbonylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D015398:Signal Transduction; D013997:Time Factors
D018797:Cell Cycle Proteins; D005632:Fructose; D005947:Glucose; D019869:Phosphatidylinositol 3-Kinases; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; C083324:TOR1 protein, S cerevisiae; C081135:TOR2 protein, S cerevisiae