An integrative process of bioconversion of oil palm empty fruit bunch fiber to ethanol with on-site cellulase production.
The aim of this study was to efficiently convert oil palm empty fruit bunch fiber (OPEFB), one of the most commonly generated lingo-wastes in Southeast Asia, into both cellulase and bioethanol. The unprocessed cellulase crude (37.29%) produced under solid-state fermentation using OPEFB as substrate showed a better reducing sugar yield using filter paper than the commercial enzyme blend (34.61%). Organosolv pretreatment method could efficiently reduce hemicellulose (24.3-18.6%) and lignin (35.2-22.1%) content and increase cellulose content (40.5-59.3%) from OPEFB. Enzymatic hydrolysis of pretreated OPEFB using the crude cellulase with 20% solid content, enzyme loading of 15 FPU/g OPEFB at 50 °C, and pH 5.5 resulted in a OPEFB hydrolysate containing 36.01 g/L glucose after 72 h. Fermentation of the hydrolysate medium produced 17.64 g/L ethanol with 0.49 g/g yield from glucose and 0.088 g/g yield from OPEFB at 8 h using Saccharomyces cerevisiae.
Department of Biological Science, Jining Medical University, Jining, 272067, China.
Youshuang
Y
Zhu
Fengxue
F
Xin
Ying
Y
Zhao
Yunkang
Y
Chang
D028023:Arecaceae / Q000737:chemistry*; D057005:Bioengineering; D056804:Biofuels; D001711:Biotransformation; D002480:Cellulase / Q000096:biosynthesis*; D000431:Ethanol / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry; D006868:Hydrolysis; D007218:Industrial Microbiology; D008031:Lignin / Q000378:metabolism; D000073878:Palm Oil; D010938:Plant Oils; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology; D014866:Waste Products / Q000032:analysis
D056804:Biofuels; D010938:Plant Oils; D014866:Waste Products; C036909:lignocellulose; D000431:Ethanol; D000073878:Palm Oil; D008031:Lignin; D002480:Cellulase