Simultaneous determination of seven synthetic colorants in wine by dispersive micro-solid-phase extraction coupled with reversed-phase high-performance liquid chromatography.
A novel and effective dispersive micro-solid-phase extraction (d-µ-SPE) using ethanediamine-functionalized magnetic Fe3O4 polymer (EDA-MP) as an efficient adsorbent in wine sample was developed. Based on this, a simple and time-saving analytical method for the simultaneous determination of seven synthetic colorants (i.e., tartrazine, amaranth, carmine, sunset yellow, allura red, brilliant blue and erythrosine) in wine by reversed-phase high-performance liquid chromatography with an ultraviolet detector was established. The experimental parameters, including the chromatographic retention behavior of studied synthetic colorants, the effect of the usage amount of cross-linking monomer, the effect of the usage amount of EDA-MP on the recovery and the recyclability of the adsorbents, were studied in detail. The results showed that the EDA-MP could be reused efficiently at least six times. Under optimized conditions, the recoveries for all analytes were in the range of 88.6-105.2%, with the intraday relative standard deviations (RSDs) ranging from 2.1 to 8.2% and the interday RSDs ranging from 3.4 to 8.7%, and all the analytes had good linearities in the tested ranges with correlation coefficients (r(2)) >0.9995. The limits of quantification for seven synthetic colorants were between 0.12 and 0.45 mg L(-1). The developed method was successfully applied to wine samples, and it was confirmed that the EDA-MP particles were highly effective d-µ-SPE materials.
Quzhou Center for Disease Control and Prevention, Quzhou 324000, PR China.
Yun
Y
Zhang
Quzhou Center for Disease Control and Prevention, Quzhou 324000, PR China.
Hua
H
Zhou
Quzhou Center for Disease Control and Prevention, Quzhou 324000, PR China.
Yougang
Y
Wang
Quzhou Center for Disease Control and Prevention, Quzhou 324000, PR China.
Xianglun
X
Wu
Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, PR China zhyg91213@163.com.
Yonggang
Y
Zhao
D000327:Adsorption; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005029:Ethylenediamines / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry; D005505:Food Coloring Agents / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*; D014920:Wine / Q000032:analysis*
D005029:Ethylenediamines; D005505:Food Coloring Agents; C031234:ethylenediamine; D052203:Ferrosoferric Oxide