Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers.
The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a atisfactory agreement with increased tolerance to environmental stress conditions of winter rape.
Engelgardt Institute of Molecular Biology of RAS, Vavilov Str. 32, Moscow 119991, Russia. amomar@mail.ru.
Alexandra V
AV
Amosova
Lyudmila V
LV
Zemtsova
Zoya E
ZE
Grushetskaya
Tatiana E
TE
Samatadze
Galina V
GV
Mozgova
Yadviga E
YE
Pilyuk
Valentina T
VT
Volovik
Natalia V
NV
Melnikova
Alexandr V
AV
Zelenin
Valentina A
VA
Lemesh
Olga V
OV
Muravenko
D001937:Brassica / Q000235:genetics* / Q000378:metabolism; D002871:Chromosome Banding; D032461:Chromosomes, Plant; D020732:Cytogenetic Analysis; D004883:Erucic Acids / Q000378:metabolism; D059647:Gene-Environment Interaction; D017343:Genes, Plant; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D059785:Karyotype; D011110:Polymorphism, Genetic; D012621:Seasons
D004883:Erucic Acids; D005819:Genetic Markers; C049811:erucic acid