Convenient detection of HPV virus in a clinical sample using concurrent rolling circle and junction probe amplifications.
Herein we show that two isothermal amplification strategies, rolling circle amplification and junction probe strategy, can be used in tandem in the same tube under isothermal conditions to detect HPV16 in clinical cervical swabs. It was discovered that the prior treatment of the clinical sample with a cocktail of restriction endonucleases (REAses) to digest the genomic DNA facilitated the isothermal detection assay.
Changzhou Fangyuan Pharmaceutical Co., Ltd., 108 Hehaixi Road, Xinbei District, Changzhou, Jiangsu 213022, China. leiyanzju@gmail.com.
Lei
L
Yan
Kailong
K
Liu
Herman O
HO
Sintim
D002584:Cervix Uteri / Q000821:virology; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques
D015344:DNA Probes, HPV; D004279:DNA, Viral