Peimisine and peiminine production by endophytic fungus Fusarium sp. isolated from Fritillaria unibracteata var. wabensis.
Steroidal alkaloids, as the major biologically active components in Bulbus Fritillariae, possess a variety of toxicological and pharmacological effects on humans. The objective of this work was to determine whether endophytic fungi isolated from fresh bulbs of Fritillaria unibracteata var. wabensis can produce one or more alkaloids like its host plant. Four classical reagents including Wagner's, iodine-potassium iodide, Mayer's and improved Dragendorff's were used for primary screening. Then thin-layer chromatography (TLC) and high performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) were employed to identify the fermentation products of the selected strains. The results showed that extract from one stain (WBS007) has positive reactions in process of primary screening. A further TLC scan and HPLC-ELSD showed that strain WBS007 had two components with the same TLC relative front (Rf) value and HPLC retention time (RT) as authentic peimisine and peiminine. In addition, strain WBS007 was identified as Fusarium sp. based on phylogenetic analysis of ITS sequences. Thus, strain WBS007 produced the bioactive ingredient peimisine and peiminine, as does its host plant, and could be used for the production of peimisine and peiminine by fermentation.
Agronomy College, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, PR China.
Feng
F
Pan
Agronomy College, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, PR China.
Kai
K
Hou
Agronomy College, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, PR China.
Feng
F
Gao
Agronomy College, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, PR China.
Bo
B
Hu
Agronomy College, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, PR China.
Que
Q
Chen
Agronomy College, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, PR China. Electronic address: ewuwei@gmail.com.
Wei
W
Wu
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002595:Cevanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D016000:Cluster Analysis; D031410:Fritillaria / Q000382:microbiology*; D005670:Fusarium / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D000470:Alkaloids; D002595:Cevanes; C047331:peiminine
Endophytic fungi; Fritillaria unibracteata var. wabuensis; Fusarium sp.; Peimisine and peiminine