Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α.
Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with a predicted phytase activity of 4194.45 U/mL. The model was validated and the results showed no significant difference between the experimental and the predicted phytase activity (P = 0.305). Under optimum cultivation conditions, the phytase activity of the recombinant E. coli DH5α was 364 times higher compared to the phytase activity of the wild-type producer, Enterobacter sakazakii ASUIA279. Hence, optimization of the cultivation conditions using RSM positively increased phytase production from recombinant E. coli DH5α.
Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Rafidah Mohd
RM
Ariff
Department of Mathematics, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Anwar
A
Fitrianto
Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Mohd Yazid
MY
Abd Manap
Department of Science Clinical Study, Faculty of Veterinary Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Aini
A
Ideris
Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Azhar
A
Kassim
Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Afinah
A
Suhairin
Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Anis Shobirin Meor
AS
Hussin
Escherichia coli; cultivation conditions; optimization; phytase; recombinant; response surface methodology