Assessment of crystallinity in processed sucrose by near-infrared spectroscopy and application to lyophiles.
The objective of this study was to investigate the capability of near-infrared spectroscopy (NIRS) to determine crystallinity in processed sucrose using a common set of calibration standards derived from binary physical mixtures. NIRS was applied as a primary method using binary mixtures of amorphous and crystalline standards to predict crystallinity in sucrose that was either rendered partially amorphous by milling, partially recrystallized from the amorphous phase, or amorphous lyophiles annealed to induce recrystallization. Crystallinity prediction in the case of milled crystalline and recrystallized amorphous sucrose was feasible using the two-state binary calibration mixtures applying a univariate model. NIRS results for milled sucrose were comparable to those obtained using X-ray powder diffraction. The changes in crystallinity after milling and recrystallization showed expected trends. However, the same NIRS univariate calibration method could not be successfully applied for directly through the vial. To overcome this complication, NIRS was applied as a secondary method relative to water vapor sorption (WVS) where a set of processed samples were measured using both NIRS and WVS and a partial least-squares model applied. The NIRS secondary method was successfully applied and provided a standard error of calibration of 2.11% and standard error of prediction of 3.76%.
College of Pharmacy, Division of Pharmaceutics, The University of Iowa, Iowa City, Iowa 52242; Boehringer Ingelheim Pharmaceuticals Inc, Pharmaceutical Research and Development, Ridgefield, Connecticut 06877. Electronic address: paul.luner@boehringer-ingelheim.com.
Paul E
PE
Luner
College of Pharmacy, Division of Pharmaceutics, The University of Iowa, Iowa City, Iowa 52242; Pfizer Consumer Healthcare, Richmond, Virginia 23261.
Jeffery J
JJ
Seyer
D002138:Calibration; D003460:Crystallization / Q000379:methods; D005612:Freeze Drying / Q000379:methods; D011208:Powders / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D013395:Sucrose / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods
D011208:Powders; D013395:Sucrose
amorphous; crystallinity; crystallization; degree of crystallinity; diffuse reflectance; lyophilization; milling; moisture sorption; near-infrared spectroscopy; water sorption