Effect of anti-oxidants, Ricetrienol and α-tocopherol, on adipocytokine abnormalities and fatty liver in Otsuka Long-Evans Tokushima Fatty diabetic rats.
BACKGROUND
We investigated the effect of Ricetrienol, which is an anti-oxidant extracted from rice bran, and α-tocopherol on the adipocytokine abnormalities and fatty liver in Otsuka Long-Evans Tokushima Fatty (OLETF) rats.


METHODS
A total of 18 OLETF rats were bred using a 30% sucrose solution (the diabetic group; DM), whereas another 18 OLETF rats were bred using ordinary water (the non-diabetic obese group; OB) as drinking water, respectively. After the sucrose-fed rats developed diabetes, all of the rats from the diabetic and obese groups were randomly divided into three groups. Then each group was fed either standard chow (DM-S, OB-S group), 0.05% Ricetrienol-containing chow (DM-R, OB-R group) or 0.05%α-tocopherol-containing chow (DM-A, OB-A group), respectively. After 12 weeks of feeding, all the rats were killed. Plasma insulin, adiponectin, resistin and leptin were assayed by enzyme immunoassay. Histopathological findings of liver tissue were scored according to Brunt and Kleiner's method, and triglyceride contents of the liver tissue were investigated.


RESULTS
Plasma adiponectin was significantly reduced in DM-S compared with OB-S, but it had significantly increased in DM-R and DM-A as opposed to DM-S. Plasma resistin showed a significant increase in DM-S compared with OB-S, but it was significantly reduced in DM-A than in DM-S. Though the triglyceride contents of liver tissue significantly increased in DM-S as opposed to OB-S, they were significantly reduced in DM-R compared with DM-S. Histopathological scores were significantly higher in DM-S than OB-S.


CONCLUSIONS
The present study shows that Ricetrienol might prevent adipocytokine abnormalities and fatty liver in OLETF diabetic rats. (J Diabetes Invest, doi: 10.1111/j.2040-1124.2010.00090.x, 2011).
The First Department of Medicine, Wakayama Medical University.
Kunihiro
K
Tatsumi
The First Department of Medicine, Wakayama Medical University.
Hideyuki
H
Sasaki
The First Department of Medicine, Wakayama Medical University.
Atsuyo
A
Fujita
The First Department of Medicine, Wakayama Medical University.
Asako
A
Doi
Research and Development Department, Tsuno Food Industrial Co. Ltd.
Yumi
Y
Kanaya
The First Department of Medicine, Wakayama Medical University.
Hiroto
H
Furuta
The First Department of Medicine, Wakayama Medical University.
Masahiro
M
Nishi
Research and Development Department, Tsuno Food Industrial Co. Ltd.
Takuo
T
Tsuno
Chemical Technology Division, Wakayama Prefectural Industrial Technique Center, Wakayama, Japan.
Hisaji
H
Taniguchi
The First Department of Medicine, Wakayama Medical University.
Kishio
K
Nanjo
Adipocytokine; Anti‐oxidant; Fatty liver