Emergence and prevalence of non-H2S-producing Salmonella enterica serovar Senftenberg isolates belonging to novel sequence type 1751 in China.
Salmonella enterica serovar Senftenberg is a common nontyphoidal Salmonella serotype which causes human Salmonella infections worldwide. In this study, 182 S. Senftenberg isolates, including 17 atypical non-hydrogen sulfide (H2S)-producing isolates, were detected in China from 2005 to 2011. The microbiological and genetic characteristics of the non-H2S-producing and selected H2S-producing isolates were determined by using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and clustered regularly interspaced short palindromic repeat (CRISPR) analysis. The phs operons were amplified and sequenced. The 17 non-H2S-producing and 36 H2S-producing isolates belonged to 7 sequence types (STs), including 3 new STs, ST1751, ST1757, and ST1758. Fourteen of the 17 non-H2S-producing isolates belonged to ST1751 and had very similar PFGE patterns. All 17 non-H2S-producing isolates had a nonsense mutation at position 1621 of phsA. H2S-producing and non-H2S-producing S. Senftenberg isolates were isolated from the same stool sample from three patients; isolates from the same patients displayed the same antimicrobial susceptibility, ST, and PFGE pattern but could be discriminated based on CRISPR spacers. Non-H2S-producing S. Senftenberg isolates belonging to ST1751 have been prevalent in Shanghai, China. It is possible that these emerging organisms will disseminate further, because they are difficult to detect. Thus, we should strengthen the surveillance for the spread of this atypical S. Senftenberg variant.
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China Xiangya Basic Medical College, Central South University, Changsha, China.
Shengjie
S
Yi
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Jing
J
Xie
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Nan
N
Liu
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Peng
P
Li
Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China.
Xuebin
X
Xu
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Hao
H
Li
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Jichao
J
Sun
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Jian
J
Wang
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Beibei
B
Liang
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Chaojie
C
Yang
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Xu
X
Wang
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Rongzhang
R
Hao
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Ligui
L
Wang
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Zhihao
Z
Wu
Department of Food Science Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Jianmin
J
Zhang
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Yong
Y
Wang
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Liuyu
L
Huang
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Yansong
Y
Sun
International Emerging Infections Program, US Centers for Disease Control and Prevention, Beijing, China Global Disease Detection Branch, Division of Global Health Protection, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
John D
JD
Klena
Department of Food Science Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China Department of Food Science and Nutrition, University of Maryland, College Park, Maryland, USA.
Jianghong
J
Meng
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China hongbinsong@263.net qiushf0613@hotmail.com.
Shaofu
S
Qiu
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China hongbinsong@263.net qiushf0613@hotmail.com.
Hongbin
H
Song
D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D018389:Codon, Nonsense; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D050505:Mutant Proteins / Q000235:genetics; D015995:Prevalence; D012480:Salmonella Infections / Q000453:epidemiology* / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D055815:Young Adult
D001426:Bacterial Proteins; D018389:Codon, Nonsense; D004269:DNA, Bacterial; D050505:Mutant Proteins; D006862:Hydrogen Sulfide