Chrebp regulates the transcriptional activity of androgen receptor in prostate cancer.
Androgen receptor (AR), a member of nuclear hormone receptor, plays an essential role in the initiation and progression of prostate cancer (PCa). In the present study, by way of immunoprecipitation followed by mass spectrometry (IP/MS) system, we found that carbohydrate-responsive element-binding protein (Chrebp), a glucose sensor in normal and cancer cells, interacted with AR in LNCaP cells. The interaction was further confirmed by coimmunoprecipitation analysis. Besides, Chrebp is required for the optimal transcriptional activity of AR in promoting the transcription of the prostate-specific antigen (PSA) promoter and messenger RNA (mRNA) expression. Consistently, knockdown of Chrebp using small interfering RNA (siRNA) in LNCaP cells reduced endogenous PSA levels. Together, our study demonstrates that Chrebp interacts with AR and regulates its transcriptional activity.
Department of Urology, East Hospital, Tongji University School of Medicine, Jimo Road 150, 200120, Shanghai, People's Republic of China.
Xue-Lei
XL
Wang
Xiao-Fei
XF
Wen
Rong-Bing
RB
Li
Bin
B
Liu
Guang-Ming
GM
Qiu
Ji-Ling
JL
Wen
Yue-Min
YM
Wang
D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000502:physiology*; D045744:Cell Line, Tumor; D006801:Humans; D047468:Immunoprecipitation; D008297:Male; D011401:Promoter Regions, Genetic; D017430:Prostate-Specific Antigen / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics*; D011944:Receptors, Androgen / Q000502:physiology*; D014158:Transcription, Genetic
C410798:AR protein, human; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C116385:MLXIPL protein, human; D011944:Receptors, Androgen; D017430:Prostate-Specific Antigen