Ethanol determination using pressure mobilization and free solution capillary electrophoresis by photo-oxidation assisted ultraviolet detection.
Free solution capillary electrophoresis (CE) can separate and quantify carbohydrates using a simple direct UV detection based on a photo-oxidation reaction taking place in the detection window without any labeling. Ethanol interferes with this photo-oxidation reaction. We thus present the first detection and quantification of ethanol using either a simple pressure mobilization set-up or CE. Ethanol can be detected down to 34.9mgL(-1) and quantified in the range 117-1850mgL(-1) through the interference with photo-oxidization of 2gL(-1) sucrose. CE can thus separate and quantify both carbohydrates and ethanol, for example to monitor a lignocellulosic fermentation process. The method is not limited to ethanol and applies to alkyl amines and other alcohols and likely to most molecules possessing the ability to react with free radicals assuming they can be sufficiently separated from each other.
University of Western Sydney (UWS), Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Locked Bag 1797, Penrith, NSW 2751, Australia. Electronic address: james.oliver@uws.edu.au.
James D
JD
Oliver
University of Western Sydney (UWS), Molecular Medicine Research Group (MMRG), School of Science and Health, Locked Bag 1797, Penrith, NSW 2751, Australia. Electronic address: m.gaborieau@uws.edu.au.
Marianne
M
Gaborieau
University of Western Sydney (UWS), Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Locked Bag 1797, Penrith, NSW 2751, Australia. Electronic address: p.castignolles@uws.edu.au.
Patrice
P
Castignolles
D002241:Carbohydrates / Q000737:chemistry; D019075:Electrophoresis, Capillary; D000431:Ethanol / Q000032:analysis*; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D010944:Plants / Q000737:chemistry; D011312:Pressure; D012996:Solutions; D014466:Ultraviolet Rays
D002241:Carbohydrates; D012996:Solutions; D000431:Ethanol
Capillary electrophoresis; Ethanol detection; Nuclear magnetic resonance spectroscopy; Photo-oxidation detection; Pressure mobilization