The influence of selenium addition during germination of Brassica seeds on health-promoting potential of sprouts.
The correlation among selenium uptake, the content of bioactive compounds in sprouts, and biological activities triggered in cultured human cells by sprout extracts was investigated. Seeds of Brassica crops and rye were treated with SeO2 water solution. The selenium levels in sprouts increased from 1.0-4.1 to 53.3-382 μg/g dw with no influence on plant physiology according to the indices used. Neither the composition of glucosinolates (GL) in Brassica sprouts nor the myrosinase activity nor the composition of GL breakdown lipophilic products were significantly affected. In all Brassica sprouts, conversion to health-promoting isothiocyanates (ITC) and indoles corresponded to only 1% of total GLs. Low ITC concentration may explain observed lack of induction of glutathione S-transferases (GST) and quinone oxidoreductase (NQO) detoxifying enzymes in HT29 cells exposed to sprout extracts. The insignificant impact on cell growth and genome function suggests that Brassica sprouts may be safe vehicle of selenium to combat its dietary deficiency.
Faculty of Chemistry, Gdansk University of Technology , Gdansk , Poland and.
Anna
A
Piekarska
Dominik
D
Kołodziejski
Tadeusz
T
Pilipczuk
Małgorzata
M
Bodnar
Piotr
P
Konieczka
Barbara
B
Kusznierewicz
Franziska S
FS
Hanschen
Monika
M
Schreiner
Joanna
J
Cyprys
Milena
M
Groszewska
Jacek
J
Namieśnik
Agnieszka
A
Bartoszek
D016588:Anticarcinogenic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D001937:Brassica / Q000378:metabolism*; D018929:Cell Culture Techniques; D004032:Diet; D018525:Germination; D005961:Glucosinolates / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D007211:Indoles / Q000378:metabolism / Q000494:pharmacology; D017879:Isothiocyanates / Q000378:metabolism*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D036226:Seedlings / Q000378:metabolism*; D012643:Selenium / Q000172:deficiency / Q000378:metabolism*; D064588:Selenium Oxides / Q000378:metabolism
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D005961:Glucosinolates; D007211:Indoles; D017879:Isothiocyanates; D010936:Plant Extracts; D064588:Selenium Oxides; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human; D005982:Glutathione Transferase; D006026:Glycoside Hydrolases; C022739:thioglucosidase; D012643:Selenium
Brassica sprouts; glucosinolate degradation products; myrosinase; phase II enzymes; selenium fortification