Fe(III)-TAML activator: a potent peroxidase mimic for chemiluminescent determination of hydrogen peroxide.
Efforts to replace native peroxidase with its low molecular weight alternatives have stimulated a search for peroxidase mimetics. Herein we describe the oxidation of luminol with hydrogen peroxide catalyzed by commercially available Fe(III)-TAML activator 1a, which was shown to be a more active catalyst than hemin. At Fe(III)-TAML activator 1a use in chemiluminescent assay for H2O2 determination the detection limit value (3σ) of 5×10(-8)M was similar to the detection limit obtained with horseradish peroxidase (1×10(-7)M) and significantly lower than that obtained in the presence of hemin (6×10(-7)M). The linear ranges (R(2)=0.98) of the assay were 6×10(-8)-1×10(-6)M and 6×10(-7)-1×10(-6)M H2O2 for Fe(III)-TAML 1a and hemin, respectively. The CV values for Fe(III)-TAML 1a-based assay measured within the working range varied from 1.0% to 3.7% (n=4), whereas in the case of hemin -5.0% to 9.7% (n=4). Moreover, the sensitivity of Fe(III)-TAML 1a-based method was 56 and 5 times higher than that of hemin- and HRP-based methods, respectively. The obtained results open good perspectives to apply Fe(III)-TAML activator 1a in CL analytical methods instead of hemin, a traditionally used peroxidase mimetic.
Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia.
Marina M
MM
Vdovenko
Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia.
Alexandra S
AS
Demiyanova
Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia.
Kirill E
KE
Kopylov
Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia. Electronic address: sakharovivan@gmail.com.
Ivan Yu
IY
Sakharov
D015374:Biosensing Techniques; D002138:Calibration; D002384:Catalysis; D004305:Dose-Response Relationship, Drug; D006427:Hemin / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D049449:Luminescence; D008165:Luminol / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry*; D010544:Peroxidases / Q000737:chemistry*; D015203:Reproducibility of Results
D008024:Ligands; D008165:Luminol; D006427:Hemin; D006861:Hydrogen Peroxide; D010544:Peroxidases; D010100:Oxygen
Chemiluminescence; Hemin; Hydrogen peroxide; Mimetics; Peroxidase; TAML