Different frequency of epidermal growth factor rs76189946 polymorphism genotype in an Iranian colorectal cancer.
OBJECTIVE
This study aimed to determinant association between rs76189946 polymorphism of EGF gene and risk of colorectal cancer in an Iranian population.


BACKGROUND
Colorectal cancer (CRC) is the third most prevalent cancer in both genders worldwide. The determination of genetic variation becomes a new way to etiology of colorectal cancer. Epidermal growth factor (EGF) is a mitogen that plays an important role in cell growth and tumourigenesis, this protein acts by binding its receptor, EGFR.


METHODS
DNA samples taken from totally 125 CRC patients and healthy controls were amplified by polymerase chain reaction (PCR) for the rs76189946 polymorphism. Genotypes were analyzed using restriction fragment length polymorphism (RFLP). Finally to confirm the RFLP procedure, 20 of the PCR products were sequenced using the ABI PRISM 3130xl Genetic Analyzer and chain termination method (Applied Biosystems, Carlsbad, CA).


RESULTS
Genotype distribution and allele frequency was similar in CRC patients and controls individuals. We expect observe C and G allele in both groups but only was found C allele.


CONCLUSIONS
In this study for first time we identified genetic distribution of exonic rs76189946 polymorphism in EGF gene both CRC patients and healthy controls. These results suggest there wasn't association between EGF polymorphism rs76189946 and risk of colorectal cancer in an Iranian population.
Basic and Molecular Epidemiology of Gastroenterology Disorder Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Vahid
V
Chaleshi
Basic and Molecular Epidemiology of Gastroenterology Disorder Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Sanaz
S
Savabkar
Basic and Molecular Epidemiology of Gastroenterology Disorder Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hesamodin
H
Dolatmoradi
Department of Biology, Science Faculty, Islamic Azad University, East Tehran Branch, Tehran, Iran.
Mahdi Montazer
MM
Haghighi
Basic and Molecular Epidemiology of Gastroenterology Disorder Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Elmira Khalili
EK
Maryan
Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Sara
S
Pasha
Basic and Molecular Epidemiology of Gastroenterology Disorder Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Narges Ebrahimi
NE
Vandfaez
Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Ehsan
E
Nazemalhosseini Mojarad
Basic and Molecular Epidemiology of Gastroenterology Disorder Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hamid Asadzadeh
HA
Aghdaei
Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammad Reza
MR
Zali
Colorectal cancer; Epidermal growth factor (EGF); Single nucleotide polymorphism (SNP); rs76189946