Characterization of a novel carbonic anhydrase from freshwater pearl mussel Hyriopsis cumingii and the expression profile of its transcript in response to environmental conditions.
Gene encoding for α-carbonic anhydrases (α-CAs) and their functions in fundamental metabolism and biomineralization are widely identified in mollusks. However, the transcriptional regulation of α-CA genes in response to various environmental conditions remains unknown. In the present study, we characterized a cDNA encoding for an α-CA (HcCA) from the freshwater pearl mussel Hyriopsis cumingii. The spatial and temporal expression patterns of HcCA indicate that this gene is mainly expressed in the mantle of juvenile mussels. The expression profile of HcCA under various environmental conditions reveals that the transcription of HcCA is significantly regulated by Ca(2+) concentration, water temperature, pH and air exposure. Our results suggest that HcCA is a crucial target gene by which the external environmental conditions affecting shell growth and pH homeostasis of H. cumingii.
College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China; College of Life Science, Shaoxing University, Shaoxing 312000, PR China.
Gang
G
Ren
College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address: ywang@zju.edu.cn.
Yan
Y
Wang
School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.
Jianguang
J
Qin
College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China.
Jinyu
J
Tang
College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China.
Xiafei
X
Zheng
College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China.
Youming
Y
Li
D020121:5' Untranslated Regions; D000367:Age Factors; D000388:Air; D000595:Amino Acid Sequence; D060105:Animal Shells / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000235:genetics* / Q000378:metabolism; D004777:Environment; D005618:Fresh Water; D015971:Gene Expression Regulation, Enzymologic; D006706:Homeostasis / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D049876:Unionidae / Q000201:enzymology* / Q000235:genetics
D020121:5' Untranslated Regions; D002256:Carbonic Anhydrases; D002118:Calcium
Carbonic anhydrase; Expression; Hyriopsis cumingii; Shell growth; pH homeostasis