Leptin level and oxidative stress contribute to obesity-induced low testosterone in murine testicular tissue.
OBJECTIVE
This study evaluated the effects of obesity on the function of reproductive organs in male mice and the possible mechanism of male secondary hypogonadism (SH) in obesity.


METHODS
Ninety-six mice were randomly assigned to three groups: the control group, diet-induced obesity group, and diet-induced obesity resistant group for 8 weeks and 19 weeks. The effects of short- and long-term high-fat diet on the reproductive organs were determined by measuring sperm count and motility, relative testis weight, testosterone level, pathological changes and apoptosis of Leydig cells. Oxidative stress was evaluated by determining malondialdehyde, H2O2, NO levels, and GSH in testis tissues. CAT, SOD, GSH-Px and Nrf2 mRNA were measured by real-time PCR.


RESULTS
Short- and long-term high-fat diet decreased sperm count and motility, relative testis weight, testosterone level; decreased CAT, SOD, GSH-Px and Nrf2 mRNA expression; increased MDA, H2O2, NO and leptin levels; inhibited the activity of CAT and GSH-Px enzymes. Pathological injury and apoptosis of Leydig cells were found in testis tissue.


CONCLUSIONS
Pathological damage of Leydig cells, oxidative stress in testis tissue, and high level of leptin may provide some evidence to clarify the mechanisms of male SH in obesity.
Department of Pharmacology, Shenyang Pharmaceutical University, No. 103 Wenhua Road, Shenhe District, Shenyang, Liaoning 110016, China.
Jian
J
Zhao
Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang, Liaoning 110001, China.
Lingling
L
Zhai
Department of Pharmacology, Shenyang Pharmaceutical University, No. 103 Wenhua Road, Shenhe District, Shenyang, Liaoning 110016, China.
Zheng
Z
Liu
Department of Pharmacology, Shenyang Pharmaceutical University, No. 103 Wenhua Road, Shenhe District, Shenyang, Liaoning 110016, China.
Shuang
S
Wu
Department of Pharmacology, Shenyang Pharmaceutical University, No. 103 Wenhua Road, Shenhe District, Shenyang, Liaoning 110016, China.
Liping
L
Xu
D000818:Animals; D017209:Apoptosis; D001835:Body Weight; D059305:Diet, High-Fat; D007006:Hypogonadism / Q000209:etiology; D020738:Leptin / Q000097:blood*; D007985:Leydig Cells / Q000166:cytology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000378:metabolism; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology; D013737:Testis / Q000378:metabolism* / Q000473:pathology; D013739:Testosterone / Q000097:blood*
D020738:Leptin; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D013739:Testosterone; D010088:Oxidoreductases