Metabolomics combined with chemometric tools (PCA, HCA, PLS-DA and SVM) for screening cassava (Manihot esculenta Crantz) roots during postharvest physiological deterioration.
Cassava roots are an important source of dietary and industrial carbohydrates and suffer markedly from postharvest physiological deterioration (PPD). This paper deals with metabolomics combined with chemometric tools for screening the chemical and enzymatic composition in several genotypes of cassava roots during PPD. Metabolome analyses showed increases in carotenoids, flavonoids, anthocyanins, phenolics, reactive scavenging species, and enzymes (superoxide dismutase family, hydrogen peroxide, and catalase) until 3-5days postharvest. PPD correlated negatively with phenolics and carotenoids and positively with anthocyanins and flavonoids. Chemometric tools such as principal component analysis, partial least squares discriminant analysis, and support vector machines discriminated well cassava samples and enabled a good prediction of samples. Hierarchical clustering analyses grouped samples according to their levels of PPD and chemical compositions.
Plant Morphogenesis and Biochemistry Laboratory, Plant Science Center, Federal University of Santa Catarina, Florianópolis, SC, Brazil; Postgraduate Program in Plant Genetic Resources, Federal University of Santa Catarina, Rodovia Admar Gonzaga 1346, CEP 88.034-001 Florianópolis, SC, Brazil.
Virgílio Gavicho
VG
Uarrota
Plant Morphogenesis and Biochemistry Laboratory, Plant Science Center, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
Rodolfo
R
Moresco
Plant Morphogenesis and Biochemistry Laboratory, Plant Science Center, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
Bianca
B
Coelho
Santa Catarina State Agricultural Research and Rural Extension Agency (EPAGRI), Experimental Station of Urussanga (EEUR), Rd. SC 446, Km 19 S/N, CEP 88840-000 Urussanga, Florianópolis, SC, Brazil.
Eduardo da Costa
Eda C
Nunes
Santa Catarina State Agricultural Research and Rural Extension Agency (EPAGRI), Experimental Station of Urussanga (EEUR), Rd. SC 446, Km 19 S/N, CEP 88840-000 Urussanga, Florianópolis, SC, Brazil.
Luiz Augusto Martins
LA
Peruch
Santa Catarina State Agricultural Research and Rural Extension Agency (EPAGRI), Experimental Station of Urussanga (EEUR), Rd. SC 446, Km 19 S/N, CEP 88840-000 Urussanga, Florianópolis, SC, Brazil.
Enilto de Oliveira
Ede O
Neubert
Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Miguel
M
Rocha
Plant Morphogenesis and Biochemistry Laboratory, Plant Science Center, Federal University of Santa Catarina, Florianópolis, SC, Brazil. Electronic address: mtocsy@gmail.com.
Marcelo
M
Maraschin
D000872:Anthocyanins; D005419:Flavonoids; D002365:Manihot / Q000737:chemistry*; D055432:Metabolomics / Q000379:methods*; D010636:Phenols; D018517:Plant Roots / Q000737:chemistry*
D000872:Anthocyanins; D005419:Flavonoids; D010636:Phenols
Cassava; Chemometrics; Metabolomics; Postharvest deterioration