Adenosine triphosphate acts as a paracrine signaling molecule to reduce the motility of T cells.
Organization of immune responses requires exchange of information between cells. This is achieved through either direct cell-cell contacts and establishment of temporary synapses or the release of soluble factors, such as cytokines and chemokines. Here we show a novel form of cell-to-cell communication based on adenosine triphosphate (ATP). ATP released by stimulated T cells induces P2X4/P2X7-mediated calcium waves in the neighboring lymphocytes. Our data obtained in lymph node slices suggest that, during T-cell priming, ATP acts as a paracrine messenger to reduce the motility of lymphocytes and that this may be relevant to allow optimal tissue scanning by T cells.
Humanitas Clinical and Research Center, Rozzano, Italy Department of Translational Medicine, University of Milan, Rozzano, Italy mary.wang@humanitasresearch.it antonella.viola@humanitasresearch.it.
Chiuhui Mary
CM
Wang
Humanitas Clinical and Research Center, Rozzano, Italy.
Cristina
C
Ploia
Humanitas Clinical and Research Center, Rozzano, Italy.
Fabio
F
Anselmi
INSERM, Paris, France.
Adelaida
A
Sarukhan
Humanitas Clinical and Research Center, Rozzano, Italy Department of Translational Medicine, University of Milan, Rozzano, Italy mary.wang@humanitasresearch.it antonella.viola@humanitasresearch.it.
Antonella
A
Viola
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002465:Cell Movement / Q000276:immunology*; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D018448:Models, Immunological; D019899:Paracrine Communication / Q000276:immunology* / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D058469:Receptors, Purinergic P2X / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D058469:Receptors, Purinergic P2X; D000255:Adenosine Triphosphate; D002118:Calcium
T cells; adenosine triphosphate; calcium wave; cell migration; imaging