Regulation of diacylglycerol kinase δ2 expression in C2C12 skeletal muscle cells by free fatty acids.
Decreased expression of diacylglycerol kinase (DGK) δ in skeletal muscles is closely related to the pathogenesis of type 2 diabetes. However, the regulation of DGKδ expression is not well understood. In this study, we found that myristic acid (14:0) significantly increased DGKδ2 protein expression in a dose-dependent manner (EC(50) = 0.16 mM) in mouse C2C12 myotubes. In contrast, oleic [18:1(n-9)], eicosenoic [20:1(n-9)] and erucic [22:1(n-9)] acids markedly decreased DGKδ2 expression. Myristic acid slowly enhanced DGKδ2 expression at the transcription level. Therefore, DGKδ2 expression is positively regulated by the relatively short-chain saturated fatty acid myristic acid but attenuated by n-9 monounsaturated fatty acids.
Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan.
Shizuka
S
Sakiyama
Takako
T
Usuki
Hiromichi
H
Sakai
Fumio
F
Sakane
D000818:Animals; D002478:Cells, Cultured; D019852:Diacylglycerol Kinase / Q000235:genetics* / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D013329:Structure-Activity Relationship
D005230:Fatty Acids, Nonesterified; D019852:Diacylglycerol Kinase