Detection of opsonic antibodies against Enterococcus faecalis cell wall carbohydrates in immune globulin preparations.
Three different commercially available polyvalent immune globulins (IG) were investigated for the existence of antibodies against cell wall carbohydrates of four different E. faecalis serotypes (using a cell wall carbohydrate-enzyme-linked immunosorbent assay), and whether these antibodies mediated opsonic killing (using an opsonic-killing assay). All three IG preparations contained antibodies against all four serotypes (CPS-A to CPS-D). However, only one of the three IG preparations showed opsonic killing against all four serotypes. Average killing was higher against serotypes A and B (72 and 79 %, respectively) than against serotypes C and D (30 and 37 %, respectively). Such IG preparations could play a role as an adjuvant therapeutic option in life-threatening infections with E. faecalis, particularly when resistant strains are involved.
Section of Pediatric Infectious Diseases and Rheumatology, Center for Pediatrics and Adolescent Medicine, University Medical Center Freiburg, Mathildenstr. 1, 79106, Freiburg, Germany, markus.hufnagel@uniklinik-freiburg.de.
M
M
Hufnagel
K
K
Sixel
F
F
Hammer
A
A
Kropec
I G
IG
Sava
C
C
Theilacker
R
R
Berner
J
J
Huebner
D002241:Carbohydrates / Q000276:immunology*; D002473:Cell Wall / Q000276:immunology*; D013293:Enterococcus faecalis / Q000145:classification / Q000276:immunology* / Q000502:physiology; D016908:Gram-Positive Bacterial Infections / Q000628:therapy; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*; D007167:Immunotherapy / Q000379:methods; D050296:Microbial Viability; D009895:Opsonin Proteins / Q000276:immunology*; D004364:Pharmaceutical Preparations; D065288:Serogroup
D002241:Carbohydrates; D007136:Immunoglobulins; D009895:Opsonin Proteins; D004364:Pharmaceutical Preparations