Influence of recovery method and centrifugation on epididymal sperm from collared peccaries (Pecari tajacu Linnaeus, 1758).
In order to establish protocols for gamete recovery from accidentally killed wild animals, or to take advantage of those slaughtered by captive breeders, we assess the influence of two methods on the recovery of epididymal sperm from collared peccaries, and verify the effect of centrifugation on such gametes. Genitalia from nine animals were used. For each animal, one epididymis was processed by flotation and the other was processed by retrograde flushing, both using a buffered media based on Tris. Following recovery, sperm were evaluated for motility, vigor, viability, functional membrane integrity, and morphology. A 1-mL aliquot of each sample was centrifuged, the supernatant removed, and the pellet suspended and evaluated as fresh samples. The sperm characteristics did not differ between the samples collected by flotation or retrograde flushing (P < 0.05). Centrifugation promoted an increase in head and tail defects, thus reducing the percentage of viable sperm (P < 0.05). No other parameter assessed for both methods was affected by centrifugation. In conclusion, epididymal sperm from collared peccaries can be efficiently collected through flotation or retrograde flushing, but not when either is followed by centrifugation.
Laboratory of Animal Germplasm Conservation - LCGA, Universidade Federal Rural do Semi-Árido - UFERSA, Mossoró, RN, Brazil.
José Artur Brilhante
JA
Bezerra
Andréia Maria
AM
da Silva
Gislayne Christianne Xavier
GC
Peixoto
Mariana de Araújo
Mde A
da Silva
Moacir
M
Franco de Oliveira
Alexandre Rodrigues
AR
Silva
D000818:Animals; D004822:Epididymis / Q000166:cytology* / Q000502:physiology; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D013094:Spermatozoa / Q000502:physiology*; D013552:Swine / Q000502:physiology*
Tayassu tajacu; centrifugation; collared peccary; epididymis; sperm