The effects of paradoxical sleep deprivation on amphetamine-induced behavioral sensitization in adult and adolescent mice.
Drug-induced behavioral sensitization (BS), paradoxical sleep deprivation (PSD) and adolescence in rodents are associated with changes in the mesolimbic dopaminergic system. We compared the effects of PSD on amphetamine-induced BS in adult and adolescent mice. Adult (90 days old) and adolescent (45 days old) Swiss mice were subjected to PSD for 48h. Immediately after PSD, mice received saline or 2.0mg/kg amphetamine intraperitoneally (i.p.), and their locomotion was quantified in activity chambers. Seven days later, all the animals were challenged with 2.0mg/kg amphetamine i.p., and their locomotion was quantified again. Acute amphetamine enhanced locomotion in both adult and adolescent mice, but BS was observed only in adolescent mice. Immediately after its termination, PSD decreased locomotion of both saline- and amphetamine-treated adolescent mice. Seven days later, previous PSD potentiated both the acute stimulatory effect of amphetamine and its sensitization in adolescent mice. In adult animals, previous PSD revealed BS. Our data suggest that adolescent mice are more vulnerable to both the immediate and long-term effects of PSD on amphetamine-induced locomotion. Because drug-induced BS in rodents shares neuroplastic changes with drug craving in humans, our findings also suggest that both adolescence and PSD could facilitate craving-related mechanisms in amphetamine abuse.
Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1° andar, 04023062 São Paulo, SP, Brazil; Department of Psychobiology, Universidade Federal de São Paulo, R. Napoleão de Barros, 925, 3° andar, 04024002 São Paulo, SP, Brazil.
Sonia R
SR
Kameda
Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1° andar, 04023062 São Paulo, SP, Brazil. Electronic address: danifukushiro@gmail.com.
Daniela F
DF
Fukushiro
Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1° andar, 04023062 São Paulo, SP, Brazil.
Thaís F
TF
Trombin
Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1° andar, 04023062 São Paulo, SP, Brazil.
Leandro
L
Sanday
Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1° andar, 04023062 São Paulo, SP, Brazil.
Raphael
R
Wuo-Silva
Department of Psychobiology, Universidade Federal de São Paulo, R. Napoleão de Barros, 925, 3° andar, 04024002 São Paulo, SP, Brazil.
Luis P
LP
Saito
Department of Psychobiology, Universidade Federal de São Paulo, R. Napoleão de Barros, 925, 3° andar, 04024002 São Paulo, SP, Brazil.
Sergio
S
Tufik
Department of Psychobiology, Universidade Federal de São Paulo, R. Napoleão de Barros, 925, 3° andar, 04024002 São Paulo, SP, Brazil.
Vânia
V
D'Almeida
Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1° andar, 04023062 São Paulo, SP, Brazil; Department of Psychobiology, Universidade Federal de São Paulo, R. Napoleão de Barros, 925, 3° andar, 04024002 São Paulo, SP, Brazil.
Roberto
R
Frussa-Filho
D000367:Age Factors; D000661:Amphetamine; D019969:Amphetamine-Related Disorders / Q000503:physiopathology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D004195:Disease Models, Animal; D008124:Locomotion / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D012892:Sleep Deprivation / Q000503:physiopathology*; D012895:Sleep, REM / Q000187:drug effects / Q000502:physiology*
D000697:Central Nervous System Stimulants; D000661:Amphetamine
Age; Amphetamine; Animal models; Drug abuse; Locomotion; Sleep deprivation