Thromboelastometric and platelet responses to silk biomaterials.
Silkworm's silk is natural biopolymer with unique properties including mechanical robustness, all aqueous base processing and ease in fabrication into different multifunctional templates. Additionally, the nonmulberry silks have cell adhesion promoting tri-peptide (RGD) sequences, which make it an immensely potential platform for regenerative medicine. The compatibility of nonmulberry silk with human blood is still elusive; thereby, restricts its further application as implants. The present study, therefore, evaluate the haematocompatibility of silk biomaterials in terms of platelet interaction after exposure to nonmulberry silk of Antheraea mylitta using thromboelastometry (ROTEM). The mulberry silk of Bombyx mori and clinically used Uni-Graft W biomaterial serve as references. Shortened clotting time, clot formation times as well as enhanced clot strength indicate the platelet mediated activation of blood coagulation cascade by tested biomaterials; which is comparable to controls.
1] Department of Biotechnology, Indian Institute of Technology, Kharagpur-721302, India [2].
Banani
B
Kundu
1] Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Austrian Cluster for Tissue Regeneration, Vienna, Austria [2].
Christoph J
CJ
Schlimp
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Austrian Cluster for Tissue Regeneration, Vienna, Austria.
Sylvia
S
Nürnberger
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Austrian Cluster for Tissue Regeneration, Vienna, Austria.
Heinz
H
Redl
Department of Biotechnology, Indian Institute of Technology, Kharagpur-721302, India.
S C
SC
Kundu
D000328:Adult; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects*; D012831:Bombyx; D005260:Female; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008297:Male; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000494:pharmacology; D047011:Silk / Q000737:chemistry*; D013916:Thrombelastography; D013997:Time Factors; D055815:Young Adult
D001672:Biocompatible Materials; D010975:Platelet Aggregation Inhibitors; D047011:Silk; D005349:Fibroins