Liposomes physically coated with peptides: preparation and characterization.
Physically coating liposomes with peptides of desirable functions is an economic, versatile, and less time-consuming approach to prepare drug delivery vehicles. In this work, we designed three peptides-Ac-WWKKKGGNNN-NH2 (W2K3), Ac-WWRRRGGNNN-NH2(W2R3), Ac-WWGGGGGNNN-NH2(W2G3)-and studied their coating ability on negatively charged liposomes. It was found that the coating was mainly driven by the electrostatic interaction between the peptides' cationic side groups and the acidic lipids, which also mediated the "anchoring " of Trp residuals in the interfacial region of lipid bilayers. At the same conditions, the amount of the coated W2R3 was more than that of W2K3, but the stability of the liposome coated with W2R3 was deteriorated. This was caused by the delocalized charge of the guanidinium group of arginine. The coating of the peptide rendered the liposome pH-responsive behavior but did not prominently change the phase transition temperature. The liposome coated with peptides displayed appropriate pH/temperature dual responsive characteristics and was able to release the content in a controlled manner.
Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
Cuicui
C
Su
Yuqiong
Y
Xia
Jianbo
J
Sun
Nan
N
Wang
Lin
L
Zhu
Tao
T
Chen
Yanyi
Y
Huang
Dehai
D
Liang
D000595:Amino Acid Sequence; D001120:Arginine / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D004247:DNA / Q000737:chemistry; D016503:Drug Delivery Systems; D005452:Fluoresceins / Q000737:chemistry; D019791:Guanidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D012542:Scattering, Radiation; D055672:Static Electricity; D013696:Temperature; D014867:Water / Q000737:chemistry
D005452:Fluoresceins; D008051:Lipid Bilayers; D008081:Liposomes; D010455:Peptides; D012492:Salts; D014867:Water; D004247:DNA; D001120:Arginine; D019791:Guanidine; C007740:fluorexon