Development of a paper-type tyrosinase biosensor for detection of phenolic compounds.
A low-cost, portable, and disposable paper-type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper-strip absorption method. Tyrosinase and a chromophore (3-methyl-2-benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (l-dopamine). The biosensor was responsive to phenolic compounds such as 4-chlorophenol, catechol, m-cresol, and p-cresol. The sensor showed stability for 70 days. The developed biosensor can be used for remote on-site qualitative monitoring of phenolic compounds in wastewater samples.
Department of Biological Sciences, Nanobiotechnology R&D Lab, Middle East Technical University, Ankara, Turkey; Nanobiz Ltd, METU-Technopolis, Ankara, Turkey.
Özge
Ö
Şenyurt
Füsun
F
Eyidoğan
Remziye
R
Yılmaz
M Tufan
MT
Öz
V Cengiz
VC
Özalp
Yakup
Y
Arıca
Hüseyin A
HA
Öktem
D000363:Agaricales / Q000201:enzymology; D052160:Benzothiazoles / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006835:Hydrazones / Q000737:chemistry; D007700:Kinetics; D057230:Limit of Detection; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000378:metabolism*; D010209:Paper; D010636:Phenols / Q000032:analysis* / Q000737:chemistry
D052160:Benzothiazoles; D004800:Enzymes, Immobilized; D006835:Hydrazones; D010636:Phenols; D014442:Monophenol Monooxygenase; C002536:3-methyl-2-benzothiazolone hydrazone
4-chlorophenol; catechol; cresol; l-dopamine; paper strip; phenol