Luteal cells from functional and regressing bovine corpora lutea differentially alter the function of gamma delta T cells.
The luteal microenvironment is thought to direct the function of resident immune cells to facilitate either luteal function or regression. To determine if luteal cells from functional (Days 10-12) and regressing (8 h after administration of prostaglandin F2alpha) corpora lutea (CL) induce different responses in γδ T cells, luteal cells were cocultured with autologous γδ T cells isolated from peripheral blood. Proliferation, functional phenotypes, and cytokine synthesis were analyzed by flow cytometry. To determine if the luteal cells from functional CL induce hyporesponsiveness in γδ T cells, γδ(+) cells were cocultured with midcycle luteal cells and further stimulated with concanavalin A. Coculture of γδ(+) cells with midcycle luteal cells did not inhibit concanavalin A-induced proliferation. In a proliferation assay, luteal cells from midcycle CL predominantly induced proliferation of γδ(+) WC1(-) cells (P < 0.05), while luteal cells from regressing CL predominantly induced proliferation of γδ(+)WC1(+) cells (P < 0.05). Analysis of intracellular cytokines indicated that midcycle luteal cells increased the proportion of γδ(+) cells containing interleukin 10 (P < 0.05), but reduced the proportion of γδ(+) cells containing interferon gamma (IFNG; P < 0.05). There were no changes in the proportions of γδ(+) cells synthesizing interleukin 4 or tumor necrosis factor. Unexpectedly, coculture of γδ(+) cells with luteal cells from regressing CL had no effect on any of the cytokines analyzed. These data support the hypothesis that the function of resident T cells is differentially modulated depending on the status of the CL.
Department of Animal Science, Center for Reproductive Biology and Health, Pennsylvania State University, State College, Pennsylvania.
Sadhat S
SS
Walusimbi
Department of Animal Science, Center for Reproductive Biology and Health, Pennsylvania State University, State College, Pennsylvania jlp36@psu.edu.
Joy L
JL
Pate
D000818:Animals; D002417:Cattle / Q000276:immunology*; D002418:Cattle Diseases / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D003338:Corpus Luteum / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D005260:Female; D005298:Fertility / Q000276:immunology; D016130:Immunophenotyping; D007246:Infertility / Q000276:immunology / Q000662:veterinary*; D003341:Luteolysis / Q000276:immunology / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
D016207:Cytokines; D016692:Receptors, Antigen, T-Cell, gamma-delta
WC1 phenotypes; corpus luteum; cytokines; gamma-delta T cells