Polyhydroxyalkanoates: waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture.
This integrated study shows that waste glycerol can be bio-valorized by the fabrication of electrospun scaffolds for stem cells. Human mesenchymal stem cells (hMSC) provide an interesting model of regenerating cells because of their ability to differentiate into osteo-, chrondro-, adipo- and myogenic lineages. Moreover, hMSC have modulatory properties with potential on treatment of immunologic diseases. Electrospun fiber meshes offer tunable mechanical and physical properties that can mimic the structure of the native extracellular matrix, the natural environment where cells inhabit. Following a biorefinery approach, crude glycerol directly recovered from a biodiesel post-reaction stream was fed as major C source to Cupriavidus necator DSM 545 to produce polyhydroxyalkanoates at polymer titers of 9-25g/L. Two of the P(3HB-4HB-3HV) terpolymers produced, one containing 11.4% 4HB and 3.5% 3HV and the other containing 35.6% 4HB and 3.4% 3HV, were electrospun into fibers of average diameters of 600 and 1400nm, respectively. hMSC were cultured for 7 days in both fiber meshes, showing their ability to support stem cell growth at acceptable proliferation levels. Comparative results clearly demonstrate that scaffold topology is critical, with electrospun PHA fibers succeeding on the support of significant cell adhesion and proliferation, where planar PHA films failed.
Department of Bioengineering and IBB - Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
Raphaël F
RF
Canadas
Department of Bioengineering and IBB - Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
João M B T
JM
Cavalheiro
Department of Bioengineering and IBB - Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
João D T
JD
Guerreiro
Department of Bioengineering and IBB - Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal; CiiEM - Center for Interdisciplinary Research Egas Moniz, Campus Universitário, Quinta da Granja, Monte de Caparica 2829-511, Caparica, Portugal.
M Catarina M D
MC
de Almeida
LIPHT - Laboratoire d'Ingénierie des Polymères pour les Hautes Technologies, Ecole de Chimie, Polymères et Matériaux, Université de Strasbourg, 25 rue Becquerel, 67087 Strasbourg Cedex 2, France.
Eric
E
Pollet
Department of Bioengineering and IBB - Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
Cláudia Lobato
CL
da Silva
Department of Bioengineering and IBB - Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
M M R
MM
da Fonseca
Department of Bioengineering and IBB - Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address: frederico.ferreira@ist.utl.pt.
Frederico Castelo
FC
Ferreira
D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002470:Cell Survival; D005990:Glycerol / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055595:Mechanical Phenomena; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*; D014866:Waste Products
D015415:Biomarkers; D054813:Polyhydroxyalkanoates; D014866:Waste Products; D005990:Glycerol
Electrospinning; Mesenchymal stem cell; Polyhydroxyalkanoates; Stem cell scaffolds; Waste glycerol