First identification and characterization of porcine enterovirus G in the United States.
Porcine enterovirus G (EV-G) is a member of the family Picornavirdae, genus Enterovirus. To date, eleven EV-G types (EV-G1 through EV-G11) have been identified in pigs from Asia and Europe however they have never been reported in North America. In this study, we isolated and characterized the complete genome of NP/2013/USA, an EV-G from a porcine diarrhea sample from the United States. The complete genome consists of 7,390 nucleotides excluding the 3' poly(A) tail, and has an open reading frame that encodes a 2,169 amino acid polyprotein. NP/2013/USA was most similar at the nucleotide (84%) and amino acid (95%) level to the HM131607, an EV-G1 type isolated from China in 2012.
Newport Laboratories, Inc., Worthington, Minnesota, United States of America.
Srivishnupriya
S
Anbalagan
Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, United States of America.
Richard A
RA
Hesse
Newport Laboratories, Inc., Worthington, Minnesota, United States of America.
Ben M
BM
Hause
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004771:Enteroviruses, Porcine / Q000145:classification / Q000235:genetics*; D005243:Feces / Q000821:virology; D016679:Genome, Viral / Q000235:genetics*; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine / Q000821:virology*; D014481:United States
D017931:DNA Primers