Oligonucleotide primers for specific detection of actinobacterial laccases from superfamilies I and K.
Although many putative laccase-like genes have been assigned to members of the phylum Actinobacteria, few of the related enzymes have been characterized so far. It is noteworthy, however, that this small number of enzymes has presented properties with industrial relevance. This observation, combined with the recognized biotechnological potential and the capability of this phylum to degrade recalcitrant soil polymers, has attracted attention for bioprospective approaches. In the present work, we have designed and tested primers that were specific for detection of sub-groups of laccase-like genes within actinomycetes, which corresponded to the superfamilies I and K from the classification presented by the laccase and multicopper oxidase engineering database. The designed primers have amplified laccase-like gene fragments from actinomycete isolates that were undetectable by primers available from the literature. Furthermore, phylogenetic alignments suggest that some of these fragments may belong to new laccases-like proteins, and thus emphasize the benefits of designing subgroup-specific primers.
Laboratório de Microbiologia Ambiental, Embrapa Meio Ambiente, Rodovia SP 340, Km 127,5, CP 69, Jaguariúna, SP, 13820-000, Brazil, tatirigamonte@yahoo.com.br.
Tatiana Alves Rigamonte
TA
Fernandes
Wendel Batista
WB
da Silveira
Flávia Maria Lopes
FM
Passos
Tiago Domingues
TD
Zucchi
D039903:Actinobacteria / Q000201:enzymology* / Q000235:genetics; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D042845:Laccase / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D042845:Laccase