Sleep deprivation impairs the extinction of cocaine-induced environmental conditioning in mice.
Persistence of a drug-environment conditioning induced by repeated psychostimulant treatment is thought to play a key role in the addictive cycle. In addition, sleep disorders are a common feature in patients with addictive disorders. Sleep deprivation shares similar neurobiological effects with psychostimulants. Therefore, we investigated whether sleep deprivation would impair the extinction of previously established conditioning between the drug effect and the environmental cues. Four cohorts of male adult mice underwent a behavioral sensitization procedure pairing drug (cocaine at 15 mg/kg, i.p.) or saline with environment (open-field apparatus). The extinction of conditioned locomotion was evaluated after control (home-cage maintained) or sleep deprivation (gentle handling method for 6h) conditions. Sleep deprivation both postponed the initiation and impaired the completeness of extinction of the conditioned locomotion promoted by previous drug-environment conditioning in cocaine-sensitized animals. While the cocaine control group required 5 free-drug sessions of exposure to the open-field apparatus to complete extinction of conditioned locomotion, the cocaine pre-treated group that experienced sleep deprivation before each extinction session still significantly differed from its respective control group on Day 5 of extinction. The possibility that the sleep condition can influence the extinction of a long-lasting association between drug effects and environmental cues can represent new outcomes for clinically relevant phenomena.
Department of Psychobiology, Universidade Federal de São Paulo, Brazil.
L F
LF
Berro
Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
A W
AW
Hollais
Department of Psychobiology, Universidade Federal de São Paulo, Brazil.
C L
CL
Patti
Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
D F
DF
Fukushiro
Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
E
E
Mári-Kawamoto
Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
F
F
Talhati
Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
J M
JM
Costa
Department of Psychobiology, Universidade Federal de São Paulo, Brazil.
K A
KA
Zanin
Department of Psychobiology, Universidade Federal de São Paulo, Brazil.
L B
LB
Lopes-Silva
Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
L M
LM
Ceccon
Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
R
R
Santos
Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
R
R
Procópio-Souza
Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
T F
TF
Trombin
Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
T S
TS
Yokoyama
Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
R
R
Wuo-Silva
Department of Psychobiology, Universidade Federal de São Paulo, Brazil.
S
S
Tufik
Department of Psychobiology, Universidade Federal de São Paulo, Brazil. Electronic address: ml.andersen12@gmail.com.
M L
ML
Andersen
Department of Psychobiology, Universidade Federal de São Paulo, Brazil; Department of Pharmacology, Universidade Federal de São Paulo, Brazil.
R
R
Frussa-Filho
D000818:Animals; D003042:Cocaine / Q000008:administration & dosage*; D003216:Conditioning, Operant; D008297:Male; D051379:Mice; D012892:Sleep Deprivation / Q000503:physiopathology*
D003042:Cocaine
Behavioral sensitization; Cocaine; Conditioning; Extinction; Mice; Sleep