Targeted protein surface sensors as a tool for analyzing small populations of proteins in biological mixtures.
Optical cross-reactive sensor arrays (the so-called chemical "noses/tongues") have recently been demonstrated as a powerful tool for high-throughput protein detecting and analysis. Nevertheless, applying this technology to biomarker detection is complicated by the difficulty of non-selective sensors to operate in biological mixtures. Herein we demonstrate a step toward circumventing this limitation by using self-assembled fluorescent receptors consisting of two distinct recognition motifs: specific and non-specific. When combined in an array, binding cooperatively between the specific and non-specific protein binders enables the system to discriminate among closely related isoform biomarkers even in the presence of serum proteins or within human urine.
Department of Organic Chemistry, The Weizmann Institute of Science, 76100 Rehovot (Israel).
Leila
L
Motiei
Zohar
Z
Pode
Anna
A
Koganitsky
David
D
Margulies
D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D040081:Protein Array Analysis / Q000379:methods*; D020033:Protein Isoforms / Q000032:analysis / Q000097:blood / Q000652:urine; D011506:Proteins / Q000032:analysis*; D013379:Substrate Specificity
D020033:Protein Isoforms; D011506:Proteins
cross-reactive sensor array; fluorescent probes; multivalency; pattern-based detection; protein surface recognition