Characterization of the most abundant Lactobacillus species in chicken gastrointestinal tract and potential use as probiotics for genetic engineering.
The count and diffusion of Lactobacilli species in the different gastrointestinal tract (GI) regions of broilers were investigated by quantitative real-time polymerase chain reaction, and the probiotic characteristics of six L. reuteri species isolated from broilers' GI tract were also investigated to obtain the potential target for genetic engineering. Lactobacilli had the highest diversity in the crop and the lowest one in the cecum. Compared with the lower GI tract, more Lactobacilli were found in the upper GI tract. Lactobacillus reuteri, L. johnsonii, L. acidophilus, L. crispatus, L. salivarius, and L. aviarius were the predominant Lactobacillus species and present throughout the GI tract of chickens. Lactobacillus reuteri was the most abundant Lactobacillus species. Lactobacillus reuteri XC1 had good probiotic characteristics that would be a potential and desirable target for genetic engineering.
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Lei
L
Wang
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Mingjian
M
Fang
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Yanping
Y
Hu
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Yuxin
Y
Yang
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Mingming
M
Yang
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China chenyulindk@163.com.
Yulin
Y
Chen
D000818:Animals; D002645:Chickens / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000382:microbiology*; D005818:Genetic Engineering; D019936:Probiotics; D060888:Real-Time Polymerase Chain Reaction
Lactobacillus; broiler; gastrointestinal tract; probiotic characteristics; real-time polymerase chain reaction