Profiling of the Chromatin-associated Proteome Identifies HP1BP3 as a Novel Regulator of Cell Cycle Progression.
The chromatin-associated proteome (chromatome) regulates cellular gene expression by restricting access of transcriptional machinery to template DNA, and dynamic re-modeling of chromatin structure is required to regulate critical cell functions including growth and replication, DNA repair and recombination, and oncogenic transformation in progression to cancer. Central to the control of these processes is efficient regulation of the host cell cycle, which is maintained by rapid changes in chromatin conformation during normal cycle progression. A global overview of chromatin protein organization is therefore essential to fully understand cell cycle regulation, but the influence of the chromatome and chromatin binding topology on host cell cycle progression remains poorly defined. Here we used partial MNase digestion together with iTRAQ-based high-throughput quantitative proteomics to quantify chromatin-associated proteins during interphase progression. We identified a total of 481 proteins with high confidence that were involved in chromatin-dependent events including transcriptional regulation, chromatin re-organization, and DNA replication and repair, whereas the quantitative data revealed the temporal interactions of these proteins with chromatin during interphase progression. When combined with biochemical and functional assays, these data revealed a strikingly dynamic association of protein HP1BP3 with the chromatin complex during different stages of interphase, and uncovered a novel regulatory role for this molecule in transcriptional regulation. We report that HP1BP3 protein maintains heterochromatin integrity during G1-S progression and regulates the duration of G1 phase to critically influence cell proliferative capacity.
From the ‡School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
Bamaprasad
B
Dutta
From the ‡School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
Yan
Y
Ren
From the ‡School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
Piliang
P
Hao
From the ‡School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
Kae Hwan
KH
Sim
From the ‡School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
Esther
E
Cheow
From the ‡School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
Sunil
S
Adav
From the ‡School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
James P
JP
Tam
From the ‡School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 sksze@ntu.edu.sg.
Siu Kwan
SK
Sze
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement; D002843:Chromatin / Q000378:metabolism*; D004268:DNA-Binding Proteins; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D006570:Heterochromatin / Q000378:metabolism; D006801:Humans; D008297:Male; D008819:Mice, Nude; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D000072258:Tumor Hypoxia; D059016:Tumor Microenvironment; D023041:Xenograft Model Antitumor Assays
D002843:Chromatin; D004268:DNA-Binding Proteins; C548039:HP1BP3 protein, human; D006570:Heterochromatin; D009687:Nuclear Proteins