3D-fluorescence in situ hybridization of intact, anaerobic biofilm.
FISH (fluorescence in situ hybridization) is a valuable technique to visualize and quantify localization of different microbial species within biofilms. Biofilm conformation can be altered during typical sample preparation for FISH, which can impact observations in multispecies biofilms, including the relative positions of cells. Here, we describe methods to preserve 3-D structure during FISH for visualization of an anaerobic coculture biofilm of Desulfovibrio vulgaris Hildenborough and Methanococcus maripaludis.
Department of Microbiology, Center for Biofilm Engineering, Montana State University, 173520, 109 Lewis Hall, Bozeman, MT, USA.
Kristen A
KA
Brileya
Laura B
LB
Camilleri
Matthew W
MW
Fields
D018441:Biofilms / Q000254:growth & development; D018920:Coculture Techniques / Q000379:methods; D016969:Desulfovibrio vulgaris / Q000166:cytology / Q000502:physiology*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D017017:Methanococcus / Q000166:cytology / Q000502:physiology*