Analysis of calcium signals in steering neuronal growth cones in vitro.
Calcium imaging allows us to measure the spatial and temporal changes in intracellular calcium concentration in living cells. Localized calcium elevation often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.
Laboratory for Neuronal Growth Mechanisms, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
Hiroki
H
Akiyama
Hiroyuki
H
Kamiguchi
D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D002478:Cells, Cultured; D002645:Chickens; D004867:Equipment Design; D020439:Growth Cones / Q000378:metabolism* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D020932:Nerve Growth Factor / Q000378:metabolism; D012984:Software
D020932:Nerve Growth Factor; D002118:Calcium