Tumor-specific delivery of siRNA using supramolecular assembly of hyaluronic acid nanoparticles and 2b RNA-binding protein/siRNA complexes.
Anticancer therapeutics delivering exogenous siRNA have been explored to suppress the tumor-associated genes, but several limitations of siRNA delivery such as tumor-targeted delivery, controlled siRNA release at the sites of interest, or instabilities of siRNA in physiological fluids should be preferentially addressed for its clinical applications. As an attempt to meet these criteria, we designed a supramolecular assembly, which was composed of cholesterol-bearing hyaluronic acid (HA-Chol) conjugates and 2b RNA-binding protein (2b)/siRNA complexes. In contrast to the traditional siRNA polyplexes using electrostatic interactions, HA-Chol nanoparticles, as a results of self-assembly of HA-Chol conjugates, provide the hydrophobic core that acts as the container for 2b protein/siRNA complexes, where a high affinity of 2b protein for siRNA could neutralize the negative-charged siRNA. Here, we investigated the potential of HA-Chol/2b/siRNA complexes as the siRNA carriers that provide encapsulation, protection, and targeted delivery of siRNA. The HA-Chol nanoparticles could selectively deliver 2b protein/siRNA complexes to the tumor cells with up-regulated CD44 receptors and suppress the expression of target gene. The pH-associated binding properties of siRNA for 2b proteins allowed the controlled release of siRNA in the endocytic compartments, and ultimately the released siRNA could obtain the RNAi acitivities in the cells, whereas the encapsulated 2b proteins still stayed within the HA-Chol nanoparticles. Our delivery systems demonstrate the promising potential of the efficient siRNA carriers in the anticancer therapeutic applications.
Institute of Global Environment and Department of Biology, Kyung Hee University, Dongdaemun-Gu, Seoul 130-701, South Korea.
Kyung-mi
KM
Choi
Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-Gu, Seoul 136-791, South Korea.
Mihue
M
Jang
Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-Gu, Seoul 136-791, South Korea; Department of Chemistry, Kyung Hee University, Dongdaemun-Gu, Seoul 130-701, South Korea.
Jong Hwan
JH
Kim
Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-Gu, Seoul 136-791, South Korea. Electronic address: hjahn@kist.re.kr.
Hyung Jun
HJ
Ahn
D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D020868:Gene Silencing; D005822:Genetic Vectors; D018960:Hyaluronan Receptors / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010942:Plant Viruses / Q000235:genetics; D034741:RNA, Small Interfering / Q000737:chemistry / Q000494:pharmacology*; D011946:Receptors, Antigen / Q000378:metabolism; D015854:Up-Regulation; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D002352:Carrier Proteins; C476399:Cd44 protein, mouse; D004337:Drug Carriers; D018960:Hyaluronan Receptors; D034741:RNA, Small Interfering; D011946:Receptors, Antigen; D014764:Viral Proteins; D006820:Hyaluronic Acid
Hyaluronic acid-choleresterol conjugate; Supramolecular assembly; siRNA; siRNA carrier