Differentiation of pancreatic stem and progenitor β-cells into insulin secreting cells in mice with diabetes mellitus.
We studied in vitro differentiation of pancreatic stem and progenitor cells into insulin secreting cells in the model of streptozotocin-induced diabetes in C57Bl/6 mice. Streptozotocin was shown to increase the population of pancreatic oligopotent β-cell precursors (CD45(-), TER119(-), CD133(+), and CD49f(low)) and did not affect multipotent (stem) progenitor cells (CD45(-), TER119(-), CD17(-), CD309(-)). During long-term culturing, diabetic multipotent progenitor cells showed high capacity for self-renewal. A population of dithizone-positive (insulin secreting cells) mononuclear cells was obtained releasing insulin after prolonged culturing in suspension enriched with diabetic CD45(-), TER119(-), CD17(-), and CD309(-) cells. The rate of generation of "new" insulin-producing cells and insulin release in the samples of experimental group considerably exceeded activity of the corresponding processes in the control group.
Research Institute of Pharmacology, Siberian Branch of Russian Academy of Medical Sciences, Tomsk, Russia.
E G
EG
Skurikhin
N N
NN
Ermakova
E S
ES
Khmelevskaya
O V
OV
Pershina
V A
VA
Krupin
L A
LA
Ermolaeva
A M
AM
Dygai
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002454:Cell Differentiation; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D004230:Dithizone / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000166:cytology*; D010179:Pancreas / Q000166:cytology*; D054448:Precursor Cells, B-Lymphoid / Q000166:cytology*; D013311:Streptozocin
D001786:Blood Glucose; D013311:Streptozocin; D004230:Dithizone