DKWSLLL, a versatile DXXXLL-type signal with distinct roles in the Cu(+)-regulated trafficking of ATP7B.
In the liver, the P-type ATPase and membrane pump ATP7B plays a crucial role in Cu(+) donation to cuproenzymes and in the elimination of excess Cu(+). ATP7B is endowed with a COOH-cytoplasmic (DE)XXXLL-type traffic signal. We find that accessory (Lys -3, Trp -2, Ser -1 and Leu +2) and canonical (D -4, Leu 0 and Leu +1) residues confer the DKWSLLL signal with the versatility required for the Cu(+)-regulated cycling of ATP7B between the trans-Golgi network (TGN) and the plasma membrane (PM). The separate mutation of these residues caused a disruption of the signal, resulting in different ATP7B distribution phenotypes. These phenotypes indicate the key roles of specific residues at separate steps of ATP7B trafficking, including sorting at the TGN, transport from the TGN to the PM and its endocytosis, and recycling to the TGN and PM. The distinct roles of ATP7B in the TGN and PM and the variety of phenotypes caused by the mutation of the canonical and accessory residues of the DKWSLLL signal can explain the separate or joined presentation of Wilson's cuprotoxicosis and the dysfunction of the cuproenzymes that accept Cu(+) at the TGN.
Centro Biología Molecular Severo Ochoa, Cantoblanco, 28049, Madrid, Spain.
Vasiliki
V
Lalioti
Sonia
S
Hernandez-Tiedra
Ignacio V
IV
Sandoval
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D019556:COS Cells; D027682:Cation Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D003300:Copper / Q000378:metabolism*; D000073840:Copper-Transporting ATPases; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D021382:Protein Sorting Signals; D021381:Protein Transport; D021601:trans-Golgi Network / Q000378:metabolism
C486201:Atp7a protein, mouse; D027682:Cation Transport Proteins; D021382:Protein Sorting Signals; D003300:Copper; D000251:Adenosine Triphosphatases; D000073840:Copper-Transporting ATPases
(DE)XXXLL-type signals; ATP7B; Cu+; Golgi; PM; endocytosis; endosomes; traffic