Problem-solving test: DNA manipulation with alkaline phosphatase and polynucleotide kinase.
Terms to be familiar with before you start to solve the test: 3'- and 5'-ends of DNA fragments, plasmids, restriction endonucleases, agarose gel electrophoresis, linear and circular DNA, superhelical DNA, blunt and sticky ends, DNA ligase, nuclease, [γ-(32) P] ATP, and radioactive labeling. © 2014 by The International Union of Biochemistry and Molecular Biology, 42(4):348-350, 2014.
Department of Medical Biology, Medical School, University of Pécs, H-7624 Pécs, Hungary.
József
J
Szeberényi
D000469:Alkaline Phosphatase / Q000378:metabolism; D001671:Biochemistry / Q000193:education*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D008967:Molecular Biology / Q000193:education*; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism; D011340:Problem Solving; D018794:Problem-Based Learning / Q000379:methods*; D011795:Surveys and Questionnaires
D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D000469:Alkaline Phosphatase
biotechnology education; molecular biology; nucleic acid enzymology; problem-based learning