Functional effects of alcohol withdrawal syndrome on peripheral sympathetic neurotransmission in vas deferens of adult rats.
OBJECTIVE
Alcohol withdrawal syndrome (AWS) is characterized by a set of physiological modifications triggered by abrupt withdrawal and/or decreasing consumption of ethanol (EtOH), which may manifest 16-48 h after ceasing consumption. The relationship between the effects of AWS and central and peripheral sympathetic neurotransmission is unknown. This study investigates the possible mechanisms on the sympathetic system during periods of AWS.


METHODS
Male Wistar rats were treated with EtOH (6-10 g/kg/day/v.o. 5 days). Subsequently, 1h, 24h, 48 h and 120 h after administration of the last dose of EtOH, the animals were sacrificed, and their vas deferens (VD) were removed to perform the following evaluations: (a) concentration-effect curves of sympathetic agonist; (b) activity of α2-adrenoreceptor; (c) function of voltage-dependent calcium channels (Cav); and (d) release of endogenous catecholamines measured in real time coupled to HPLC.


RESULTS
The results showed that the maximum effects of contraction were increased by agonists tested in at 24h and 48 h EtOH withdrawal. The inhibitory affinity (pIC50) of guanfacine was decreased 24h EtOH withdrawal. The function of Cav was also decreased as pIC50 values dropped 24h and 48 h EtOH withdrawal. The release of catecholamines increased 48 h after EtOH withdrawal. It is suggested that AWS triggers hyperactivity in peripheral sympathetic neurotransmission.


CONCLUSIONS
The mechanisms underlying hyperactivity are possibly explained by a failure of autoregulation from catecholamines released by α2-adrenoreceptors and/or an increase of Cav function, which may be potential targets to attenuate the symptoms of AWS at the peripheral level.
Department of Pharmacology, Federal University of São Paulo (Unifesp), Rua 3 de maio 100, 04044-020 São Paulo, SP, Brazil. Electronic address: henrique_epm@hotmail.com.
Guilherme Henrique Souza
GH
Bomfim
Department of Pharmacology, Federal University of São Paulo (Unifesp), Rua 3 de maio 100, 04044-020 São Paulo, SP, Brazil.
Luciana Ferreira
LF
Verde
Department of Pharmacology, Federal University of São Paulo (Unifesp), Rua 3 de maio 100, 04044-020 São Paulo, SP, Brazil.
Roberto
R
Frussa-Filho
Department of Pharmacology, Federal University of São Paulo (Unifesp), Rua 3 de maio 100, 04044-020 São Paulo, SP, Brazil. Electronic address: aron.farm@epm.br.
Aron
A
Jurkiewicz
Department of Pharmacology, Federal University of São Paulo (Unifesp), Rua 3 de maio 100, 04044-020 São Paulo, SP, Brazil.
Neide Hyppolito
NH
Jurkiewicz
D058647:Adrenergic alpha-2 Receptor Agonists / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D002395:Catecholamines / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage*; D016316:Guanfacine / Q000008:administration & dosage / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D018341:Receptors, Adrenergic, alpha-2 / Q000187:drug effects / Q000378:metabolism; D013375:Substance Withdrawal Syndrome / Q000503:physiopathology*; D009435:Synaptic Transmission; D013997:Time Factors; D014649:Vas Deferens / Q000378:metabolism*
D058647:Adrenergic alpha-2 Receptor Agonists; D015220:Calcium Channels; D002395:Catecholamines; D018341:Receptors, Adrenergic, alpha-2; D016316:Guanfacine; D000431:Ethanol
Alcohol withdrawal syndrome; Calcium channels; Catecholamines; Peripheral sympathetic neurotransmission; Vas deferens