Use of Quantitative Fluorescent Polymerase Chain Reaction (QF PCR) in Prenatal Diagnostic of Fetal Aneuploidies in a 17 Month Period in Parallel with Karyotyping.
BACKGROUND
QF PCR has recently entered diagnostic practice as a possible way to bypass culturing of the fetal cells, as well as to provide a rapid response following amniocentesis.


METHODS
The effective value of the QF PCR remains a much debated issue, positions ranging from that it makes classic kayotyping obsolete except in special occasions, to that it is no more than a guideline for a mandatory karyotype. Current practices of the gynecology specialists generates samples in such fashion that kariotyping of samples quickly falls behind to the point of obsoleteness, because, by the time a karyotype has been finished, a window of opportunity for termination of pregnancy has closed.


RESULTS
QF PCR provides a rapid response alternative, but it is necessary to establish its reproducibility, as well as an algorithm of its use along classic kariotyping. This study contains samples processed in a period from August 1, 2012 to December 31 2013 in both QF PCR and classic karyotype. Object of this study was compare results obtained by two methods, and establish confidence interval of the QF PCR testing. Overall, 661 amniotic fluid samples were processed and typed with QF PCR, out of which 221 were done in parallel with karyiotyping, as an confirmation of results.
Clinical Pathology, Clinical Centre, University of Sarajevo, Bosnia and Herzegovina.
Rijad
R
Konjhodzic
Clinical Microbiology, Clinical Centre, University of Sarajevo, Bosnia and Herzegovina.
Edina
E
Dervovic
Clinical Pathology, Clinical Centre, University of Sarajevo, Bosnia and Herzegovina.
Ilvana
I
Kurtovic-Basic
Clinical Pathology, Clinical Centre, University of Sarajevo, Bosnia and Herzegovina.
Meliha
M
Stomornjak-Vukadin
Clinical Pathology, Clinical Centre, University of Sarajevo, Bosnia and Herzegovina.
Adis
A
Muhic
Clinical Pathology, Clinical Centre, University of Sarajevo, Bosnia and Herzegovina.
Sumeja
S
Baljevic
Clinical Pathology, Clinical Centre, University of Sarajevo, Bosnia and Herzegovina.
Aida
A
Pirnat-Gegic
Clinic for gynecology and obstetrics, Clinical Centre, University of Sarajevo, Bosnia and Herzegovina.
Ejub
E
Basic
Clinical Pathology, Clinical Centre, University of Sarajevo, Bosnia and Herzegovina.
Nurija
N
Bilalovic
QF PCR; amniocentesis; kayotyping