First Litopenaeus vannamei WSSV 100% oral vaccination protection using CotC::Vp26 fusion protein displayed on Bacillus subtilis spores surface.
OBJECTIVE
Litopenaeus vannamei do not have an adaptive immune response system. White spot syndrome virus (WSSV) is the most severe pathogen in shrimps. Bacillus subtilis spores carrying heterologous antigens on its surface have been evaluated as a vaccine inducing specific systemic responses on vertebrates. Orally administrated Vp28 vaccines have been investigated in crustaceans. Vp26 is also an important constituent of WSSV structure but little is known about its oral vaccination capacity in L. vannamei.


RESULTS
In this study, for first time, L. vannamei WSSV protection was carried out using B. subtilis recombinant spores (RS), displaying CotC::Vp26 fusion protein (FP) on its surface. RS-expressing FP were coated on shrimp food pellets and used to feed L. vannamei. Results have shown that orally administered B. subtilis RS protected 100% L. vannamei against WSSV infection.


CONCLUSIONS
Bacillus subtilis spores orally administrated expressing CotC::Vp26 fusion protein on its surface demonstrated the great capacity of Vp26 to induce immune protection, equally or even greater than Vp28 in L. vannamei.


CONCLUSIONS
The biotechnological process developed represents an easy to produce, practical to handle, environmentally stable, human-safe and economically feasible opportunity to apply a new Vp26 vaccine in a massively way in shrimp farms.
Molecular Microbiology Laboratory, Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, México.
A
A
Valdez
G
G
Yepiz-Plascencia
E
E
Ricca
J
J
Olmos
D000284:Administration, Oral; D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D033561:Penaeidae / Q000276:immunology / Q000821:virology*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000276:immunology; D013171:Spores, Bacterial / Q000235:genetics; D014611:Vaccination / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics; D046848:White spot syndrome virus 1 / Q000276:immunology*
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines
Bacillus subtilis; Litopenaeus vannamei; WSSV; oral vaccination; spores