Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma.
The present work aimed to investigate the expression and role of aminoacylase 1 (ACY1) in hepatocellular carcinoma (HCC) based on a proteomic study. The study results revealed that the expression of ACY1 was much lower in HCC tissues. ACY1 expression significantly correlated with the serum alpha fetoprotein level and tumor invasiveness. The knockdown of ACY1 in SMMC7721 cells promoted cell viability and invasiveness. In contrast, the restoration of ACY1 in BEL7402 cells inhibited cell viability and invasiveness. Further studies indicated that the knockdown of ACY1 increased the protein expression of transforming growth factor beta 1 and extracellular signal-regulated kinase 1 expression. The study's results indicated that ACY1 acts as a tumor suppressor in HCC.
Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou 310003, China.
Xuyong
X
Wei
Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou 310003, China.
Jie
J
Li
Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou 310003, China.
Haiyang
H
Xie
Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Qi
Q
Ling
Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou 310003, China.
Jianguo
J
Wang
Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou 310003, China.
Di
D
Lu
Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou 310003, China.
Lin
L
Zhou
Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China. Electronic address: zjxu@zju.edu.cn.
Xiao
X
Xu
Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China. Electronic address: zyzss@zju.edu.cn.
Shusen
S
Zheng
D000581:Amidohydrolases / Q000502:physiology*; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D005260:Female; D055785:Gene Knockdown Techniques; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D059807:S Phase Cell Cycle Checkpoints; D053719:Tandem Mass Spectrometry; D025521:Tumor Suppressor Proteins / Q000502:physiology*; D057825:Two-Dimensional Difference Gel Electrophoresis
D020543:Proteome; D025521:Tumor Suppressor Proteins; D000581:Amidohydrolases; C019672:aminoacylase I
Aminoacylase 1; Hepatocellular carcinoma; Proteomic; Tumor suppressor