Airway epithelial cell-derived insulin-like growth factor-1 triggers skewed CD8(+) T cell polarization.
Skewed CD8(+) T cell responses are important in airway inflammation. This study investigates the role of the airway epithelial cell-derived insulin-like growth factor 1 (IGF1) in contributing to CD8(+) T cell polarization. Expression of IGF1 in the airway epithelial cell line, RPMI2650 cells, was assessed by quantitative real time RT-PCR and Western blotting. The role of IGF1 in regulating CD8(+) T cell activation was observed by coculture of mite allergen-primed RPMI2650 cells and naïve CD8(+) T cells. CD8(+) T cell polarization was assessed by the carboxyfluorescein succinimidyl ester-dilution assay and the determination of cytotoxic cytokine levels in the culture medium. Exposure to mite allergen, Der p1, increased the expression of IGF1 by RPMI2650 cells. The epithelial cell-derived IGF1 prevented the activation-induced cell death by inducing the p53 gene hypermethylation. Mite allergen-primed RPMI2650 cells induced an antigen-specific CD8(+) T cell polarization. We conclude that mite allergens induce airway epithelial cell line, RPMI2650 cells, to produce IGF1; the latter contributes to antigen-specific CD8(+) T cell polarization.
Department of Thoracic Surgery, The First Affiliated hospital, Sun Yat-Sen University, No.58, Zhongshan 2nd Road, Guangzhou, 510080, P. R. China.
Jian-Yong
JY
Zou
Shao-hong
SH
Huang
Yun
Y
Li
Hui-guo
HG
Chen
Jian
J
Rong
Sheng
S
Ye
D039741:Antigens, Dermatophagoides / Q000494:pharmacology; D060829:Arthropod Proteins / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects*; D003546:Cysteine Endopeptidases / Q000494:pharmacology; D019175:DNA Methylation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D016159:Tumor Suppressor Protein p53; D007334:Insulin-Like Growth Factor I; D048049:Extracellular Signal-Regulated MAP Kinases; D020930:MAP Kinase Kinase Kinases; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1
CD8 T cells; P53 gene methylation; airway; epithelial cells; insulin-like growth factor-1