A chemical "hub" for absolute quantification of a targeted protein: orthogonal integration of elemental and molecular mass spectrometry.
A novel element-tagged activity-based photo-cleavable biotinylated chemical "Hub" was designed and synthesized to orthogonally integrate ICP-MS and ESI-MS for absolute targeted-protein quantification. This tetrafunctional chemical "hub" allowed us to quantify a targeted protein using species-unspecific isotope dilution ICP-MS and to know which protein is being quantified using ESI-IT-MS at the same time.
Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. qqwang@xmu.edu.cn.
Xiaowen
X
Yan
Zhaoxin
Z
Li
Yong
Y
Liang
Limin
L
Yang
Bo
B
Zhang
Qiuquan
Q
Wang
D001710:Biotin / Q000737:chemistry* / Q000378:metabolism; D004987:Ethers / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007201:Indicator Dilution Techniques; D008027:Light; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000032:analysis* / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry* / Q000378:metabolism; D013441:Sulfinic Acids / Q000737:chemistry* / Q000378:metabolism
D004987:Ethers; D013441:Sulfinic Acids; D012694:Serine; C048899:sulfuryl fluoride; D001710:Biotin; D019809:Streptavidin; D010447:Peptide Hydrolases