Fungal laccases degradation of endocrine disrupting compounds.
Over the past decades, water pollution by trace organic compounds (ng/L) has become one of the key environmental issues in developed countries. This is the case of the emerging contaminants called endocrine disrupting compounds (EDCs). EDCs are a new class of environmental pollutants able to mimic or antagonize the effects of endogenous hormones, and are recently drawing scientific and public attention. Their widespread presence in the environment solicits the need of their removal from the contaminated sites. One promising approach to face this challenge consists in the use of enzymatic systems able to react with these molecules. Among the possible enzymes, oxidative enzymes are attracting increasing attention because of their versatility, the possibility to produce them on large scale, and to modify their properties. In this study five different EDCs were treated with four different fungal laccases, also in the presence of both synthetic and natural mediators. Mediators significantly increased the efficiency of the enzymatic treatment, promoting the degradation of substrates recalcitrant to laccase oxidation. The laccase showing the best performances was chosen to further investigate its oxidative capabilities against micropollutant mixtures. Improvement of enzyme performances in nonylphenol degradation rate was achieved through immobilization on glass beads.
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, Italy.
Gemma
G
Macellaro
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, Italy.
Cinzia
0000-0001-5694-472X
C
Pezzella
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, Italy.
Paola
P
Cicatiello
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, Italy.
Giovanni
G
Sannia
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, Italy.
Alessandra
0000-0002-3818-6539
A
Piscitelli
D001234:Aspergillus niger / Q000201:enzymology*; D052160:Benzothiazoles / Q000378:metabolism; D001673:Biodegradation, Environmental; D052244:Endocrine Disruptors / Q000737:chemistry / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism; D042845:Laccase / Q000378:metabolism*; D020076:Pleurotus / Q000201:enzymology*; D013451:Sulfonic Acids / Q000378:metabolism
D052160:Benzothiazoles; D052244:Endocrine Disruptors; D004800:Enzymes, Immobilized; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D042845:Laccase