Molecular characteristic and expression analysis of collagenolytic serine protease from the Chinese mitten crab Eriocheir sinensis with defense response to Vibrio anguillarum challenge.
A novel collagenolytic serine protease (CLSP) was cloned from the hemocytes of the Chinese mitten crab Eriocheir sinensis (Es-CLSP). The full-length cDNA of Es-CLSP contains 990 nucleotides. It encodes a 270-amino acid-long peptide with the mature peptide containing 221 amino acids. It contains the conserved catalytic triad (H, D, and S). Similarity analysis shows that Es-CLSP shares high identity with CLSPs from the fiddler crab Uca pugilator. Es-CLSP mRNA expression in E. sinensis is a) tissue-related with the highest expression in hemocytes and widely distributed, b) highly responsive to Vibrio anguillarum challenge in hemocytes, and c) a different response to the intruding pathogens. The results of this study demonstrate the successful isolation of Es-CLSP and indicate that Es-CLSP is an immune-related gene, and show the possible role of CLSP in the invertebrate innate immune system.
School of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan, China.
Q Z
QZ
Yang
School of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan, China.
Z J
ZJ
Yang
School of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan, China.
Y
Y
Zhang
Xinxiang Vocational and Technical College, Xinxiang, Henan, China.
X L
XL
Li
School of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan, China wzhang.cn@hotmail.com.
W
W
Zhang
D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D010802:Phylogeny; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000096:biosynthesis* / Q000235:genetics; D014733:Vibrio / Q000472:pathogenicity*; D014735:Vibrio Infections / Q000235:genetics* / Q000382:microbiology
D018076:DNA, Complementary; D012697:Serine Endopeptidases; C066053:collagenolytic protease I, crab