Recombinant thioredoxin 1 protein: the immune-adjuvant effect of Streptococcus iniae and its safety in rock bream, Oplegnathus fasciatus.
Adjuvant is an immune enhancer commonly used during vaccination to enhance the host immune response. In the present study, we analysed the recombinant protein produced from rock bream thioredoxin 1 cDNA (rRbTRx1). To verify the immune-stimulatory effect of this recombinant protein, changes in the expression level of several genes were investigated using the cDNA microarray chips in rock bream peripheral blood leukocytes stimulated with rRbTRx1. Furthermore, the immune responses of rock bream to Streptococcus iniae FKC (formalin-killed cell) vaccination alone or in combination with recombinant proteins were analysed after an experimental challenge with living S. iniae. Microarray analysis showed that 237 unique genes were upregulated more than two-fold after rRbTRx1 stimulation. Serum agglutination titres were relatively low; however, the FKC vaccine still conferred protection against S. iniae. Moreover, the adverse effects showed no statistically significant difference between fish injected with a concentration and non-injected fish. After experimental challenge to the rock bream by injection with living bacteria (S. iniae), the relative percent survival in the vaccinated groups with FKC + rRbTRx1 was significantly higher than that of the vaccinated groups with FKC alone, which were 85.9% and 68.2%, respectively. This indicated that the recombinant protein as an adjuvant showed synergism with the injected vaccine in rock bream.
Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong, Gyeongnam 650-160, Republic of Korea.
Ju-Won
JW
Kim
School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
Sang Hee
SH
Shim
Genetics and Breeding Research Center, National Fisheries Research and Development Institute, Geoje 656-842, Republic of Korea.
Jung-Ho
JH
Lee
Genetics and Breeding Research Center, National Fisheries Research and Development Institute, Geoje 656-842, Republic of Korea.
Ji-Min
JM
Jeong
Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong, Gyeongnam 650-160, Republic of Korea. Electronic address: vinus96@hanmail.net.
Chan-Il
CI
Park
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000372:Agglutination Tests / Q000662:veterinary; D000704:Analysis of Variance; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology*; D005786:Gene Expression Regulation / Q000276:immunology; D046228:Microarray Analysis / Q000662:veterinary; D010473:Perciformes; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D011994:Recombinant Proteins / Q000276:immunology* / Q000494:pharmacology; D013290:Streptococcal Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000187:drug effects / Q000276:immunology*; D013879:Thioredoxins / Q000276:immunology* / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins; D013879:Thioredoxins
Adjuvant; Recombinant protein; Rock bream; Streptococcus iniae; Vaccine