Serum matrix metalloproteinase levels in patients exposed to sulfur mustard.
BACKGROUND
Matrix metalloproteinases (MMPs) are a group of endopeptidases which comprised of various types. These proteolytic enzymes are zinc-dependent and play role in degradation of extracellular matrix (ECM). Various types of cells such as macrophages, fibroblasts, neutrophils, synovial cells and some epithelial cells secrete MMPs. According to previous studies on bronchiolitis and respiratory tract lesions in these patients and unknown pathophysiology mechanism up to date, this cross-sectional study was performed.


OBJECTIVE
The aim of this study was to compare the serum MMP level in patients with chemical injuries and normal people and also determine the role of these parameters in pulmonary disorders .


METHODS
In this cross-sectional study, 25 Iranian patients exposed to the sulfur mustard and 25 unexposed participants as the control group were enrolled. Serum samples were collected from two groups and stored at -70˚C until the measurement of MMPs and TIMPs. ELISA kit was used for measurement of MMP and TIMP based on the kit's instruction. For validations in measurement, all samples were analyzed duplicate and in some cases triplicate.


RESULTS
The mean level of MMP-9 in serum of chemically-injured group was 1592.42 and this amount in normal group was 679.72 .So there was a significant difference between two groups (P = 0.001) and the mean level of MMP-8 in serum of patients group was 49.10 and in normal group was 35.53. Then there was no significant difference between two groups (P = 0.197). The mean levels of MMP-1 and MMP-2 was not significantly different (P value > 0.05) in the patient and normal groups. And also the mean levels of TIMP-1 and TIMP-2 was not significantly different (P > 0.05) in the patients and normal groups.


CONCLUSIONS
In summary, serum MMPs in chemically-injured has shown no significant difference with normal people except for the MMP-9.
Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
Majid
M
Shohrati
Department of Biochemistry, Payame Noor University, Tehran, IR Iran.
Reza
R
Haji Hosseini
Department of Biochemistry, Payame Noor University, Tehran, IR Iran.
Malek Ashtar
MA
Esfandiari
Department of Biochemistry, Payame Noor University, Tehran, IR Iran.
Nastaran
N
Najafian
Department of Pediatrics, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
Bita
B
Najafian
Department of Biochemistry, Payame Noor University, Tehran, IR Iran.
Abbas
A
Golbedagh
Matrix Metalloproteinase; Mustard Gas; Tissue Inhibitor of Metalloproteinases