Patchouli alcohol dampens lipopolysaccharide induced mastitis in mice.
Patchouli alcohol (PA), a tricyclic sesquiterpene isolated from Pogostemonis Herba, has been known to exhibit antioxidant, anti-inflammatory, and other important therapeutic activities. The aim of this study was to investigate the effects of PA on LPS-induced mastitis in vivo and the possible mechanism. The mouse model of mastitis was induced by injection of LPS through the duct of mammary gland. Mice were pretreated with dexamethasone or PA 1 h before and 12 h after induction of LPS. The myeloperoxidase activity and inflammatory cytokines production in mammary tissues were determined. The effects of PA on NF-κB signal pathways were analyzed by Western blotting. The results showed that PA inhibited the LPS-induced TNF-α, IL-6, and IL-1β production in a dose manner. It was also observed that PA attenuated mammary histopathologic changes. Furthermore, Western blot analysis showed that PA could inhibit the phosphorylation of NF-κB and IκB induced by LPS. These results indicate that PA inhibits NF-κB signaling pathways to attenuate inflammatory injury induced by LPS. PA may be a potent therapeutic reagent for the prevention of mastitis.
Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 15 West Changle Road, Xi'an, Shan Xi, 710032, China.
Yong-Ping
YP
Li
Shi-Fang
SF
Yuan
Guo-Hong
GH
Cai
Hui
H
Wang
Ling
L
Wang
Lei
L
Yu
Rui
R
Ling
Jun
J
Yun
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000633:toxicity*; D008297:Male; D008413:Mastitis / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012717:Sesquiterpenes / Q000494:pharmacology / Q000627:therapeutic use*
D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D012717:Sesquiterpenes; C052983:patchouli alcohol