Effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 on hepatic steatosis in Zucker rats.
We have previously described the safety and immunomodulatory effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 in healthy volunteers. The scope of this work was to evaluate the effects of these probiotic strains on the hepatic steatosis of obese rats. We used the Zucker rat as a genetic model of obesity. Zucker-Lepr(fa/fa) rats received one of three probiotic strains, a mixture of L. paracasei CNCM I-4034 and B. breve CNCM I-4035, or a placebo for 30 days. An additional group of Zucker-lean+/fa rats received a placebo for 30 days. No alterations in intestinal histology, in the epithelial, lamina propria, muscular layers of the ileal or colonic mucosa, or the submucosae, were observed in any of the experimental groups. Triacylglycerol content decreased in the liver of Zucker-Lepr(fa/fa) rats that were fed L. rhamnosus, B. breve, or the mixture of B. breve and L. paracasei. Likewise, the area corresponding to neutral lipids was significantly smaller in the liver of all four groups of Zucker-Lepr(fa/fa) rats that received probiotics than in rats fed the placebo. Zucker-Lepr(fa/fa) rats exhibited significantly greater serum LPS levels than Zucker-lean+/fa rats upon administration of placebo for 30 days. In contrast, all four groups of obese Zucker-Lepr(fa/fa) rats that received LAB strains exhibited serum LPS concentrations similar to those of Zucker-lean+/fa rats. Serum TNF-α levels decreased in the Zucker-Lepr(fa/fa) rats that received B. breve, L. rhamnosus, or the mixture, whereas L. paracasei feeding decreased IL-6 levels in the serum of Zucker-Lepr(fa/fa) rats. In conclusion, the probiotic strains reduced hepatic steatosis in part by lowering serum LPS, and had an anti-inflammatory effect in obese Zucker rats.
Department of Biochemistry & Molecular Biology II, School of Pharmacy, University of Granada, Granada, Spain; Institute of Nutrition & Food Technology "José Mataix", Biomedical Research Center, University of Granada, Granada, Spain.
Julio
J
Plaza-Diaz
Department of Biochemistry & Molecular Biology II, School of Pharmacy, University of Granada, Granada, Spain; Institute of Nutrition & Food Technology "José Mataix", Biomedical Research Center, University of Granada, Granada, Spain.
Carolina
C
Gomez-Llorente
Department of Cell Biology, School of Sciences, University of Granada, Granada, Spain.
Francisco
F
Abadia-Molina
Department of Biochemistry & Molecular Biology I, School of Sciences, University of Granada, Granada, Spain.
Maria Jose
MJ
Saez-Lara
Institute of Nutrition & Food Technology "José Mataix", Biomedical Research Center, University of Granada, Granada, Spain.
Laura
L
Campaña-Martin
Department of Biochemistry & Molecular Biology II, School of Pharmacy, University of Granada, Granada, Spain; Institute of Nutrition & Food Technology "José Mataix", Biomedical Research Center, University of Granada, Granada, Spain.
Sergio
S
Muñoz-Quezada
Hero Global Technology Center, Hero Spain, S.A., Alcantarilla, Murcia, Spain.
Fernando
F
Romero
Department of Biochemistry & Molecular Biology II, School of Pharmacy, University of Granada, Granada, Spain; Institute of Nutrition & Food Technology "José Mataix", Biomedical Research Center, University of Granada, Granada, Spain.
Angel
A
Gil
Department of Biochemistry & Molecular Biology II, School of Pharmacy, University of Granada, Granada, Spain; Institute of Nutrition & Food Technology "José Mataix", Biomedical Research Center, University of Granada, Granada, Spain.
Luis
L
Fontana
D054392:Adipokines / Q000097:blood; D000818:Animals; D001644:Bifidobacterium; D016207:Cytokines / Q000097:blood; D005234:Fatty Liver / Q000628:therapy*; D007778:Lactobacillus; D008070:Lipopolysaccharides / Q000097:blood; D008297:Male; D009765:Obesity / Q000097:blood; D019936:Probiotics; D051381:Rats; D011924:Rats, Zucker
D054392:Adipokines; D016207:Cytokines; D008070:Lipopolysaccharides