Electrophoretic deposition of gentamicin-loaded bioactive glass/chitosan composite coatings for orthopaedic implants.
Despite their widespread application, metallic orthopaedic prosthesis failure still occurs because of lack of adequate bone-bonding and the incidence of post-surgery infections. The goal of this research was to develop multifunctional composite chitosan/Bioglass coatings loaded with gentamicin antibiotic as a suitable strategy to improve the surface properties of metallic implants. Electrophoretic deposition (EPD) was applied as a single-step technology to simultaneously deposit the biopolymer, bioactive glass particles, and the antibiotic on stainless steel substrate. The microstructure and composition of the coatings were characterized using SEM/EDX, XRD, FTIR, and TGA/DSC, respectively. The in vitro bioactivity of the coatings was demonstrated by formation of hydroxyapatite after immersion in simulated body fluid (SBF) in a short period of 2 days. High-performance liquid chromatography (HPLC) measurements indicated the release of 40% of the loaded gentamicin in phosphate buffered saline (PBS) within the first 5 days. The developed composite coating supported attachment and proliferation of MG-63 cells up to 10 days. Moreover, disc diffusion test showed improved bactericidal effect of gentamicin-loaded composite coatings against S. aureus compared to control non-gentamicin-loaded coatings.
Department of Materials, Imperial College London , Prince Consort Road, London SW7 2BP, United Kingdom.
Fatemehsadat
F
Pishbin
Viviana
V
Mouriño
Sabrina
S
Flor
Stefan
S
Kreppel
Vehid
V
Salih
Mary P
MP
Ryan
Aldo R
AR
Boccaccini
D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D002152:Calorimetry, Differential Scanning; D002478:Cells, Cultured; D048271:Chitosan; D020099:Coated Materials, Biocompatible; D005839:Gentamicins / Q000008:administration & dosage*; D005898:Glass; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009985:Orthopedics; D019736:Prostheses and Implants; D013818:Thermogravimetry
D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D005839:Gentamicins; D048271:Chitosan