Production of hexaoligochitin from colloidal chitin using a chitinase from Aeromonas schubertii.
Chitin derivatives, such as those with modified main saccharide chains and deacetylated side chains, exhibit versatile biological functions. The biomedical properties of chitin oligosaccharides depend on their degree of oligomerization. Of the chitin oligosaccharides, chitin hexamers are generally the most potent. In our recent study, N-acetylchitohexaose was obtained by digesting chitin with ASCHI61, a chitinase from Aeromonas schubertii. In this work, the factors involved in the production of chitin hexasaccharide were evaluated experimentally. Using steep map analysis and cross-analysis, the substrate concentration and reaction pH were identified as the key factors in this reaction, and the interactions between these parameters were observed. Using a response surface experimental design, we predicted that a colloidal chitin concentration of 3.4mgmL(-1) and a pH of 6.54 were the optimal conditions for producing hexaoligochitin. These conditions were verified in separate experiments, in which 38.73mmolL(-1) of N-acetylchitohexaose was obtained. The maximum amount of hexamer produced was 42.175mgL(-1), an increase of only 0.27% from the predicted value.
Graduate School of Biochemical Engineering and Department of Chemical Engineering, Ming Chi University of Technology, Taishan, New Taipei City, Taiwan.
Chao-Lin
CL
Liu
Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li 32003, Taiwan.
Chih-Yu
CY
Lan
Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li 32003, Taiwan.
Chuan-Chieh
CC
Fu
Department of Chemical and Materials Engineering, Chang Gung University, Kwei-Shan, Taoyuan 33302, Taiwan. Electronic address: rsjuang@mail.cgu.edu.tw.
Ruey-Shin
RS
Juang
D000333:Aeromonas / Q000201:enzymology*; D002686:Chitin / Q000737:chemistry* / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism*; D003102:Colloids; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008962:Models, Theoretical; D058105:Polymerization; D013696:Temperature
D003102:Colloids; D002686:Chitin; D002688:Chitinases
Chitinase; Colloidal chitin; Hexaoligochitin production; Response surface methodology