Increase in hypotonic stress-induced endocytic activity in macrophages via ClC-3.
Extracellular hypotonic stress can affect cellular function. Whether and how hypotonicity affects immune cell function remains to be elucidated. Macrophages are immune cells that play key roles in adaptive and innate in immune reactions. The purpose of this study was to investigate the role and underlying mechanism of hypotonic stress in the function of bone marrow-derived macrophages (BMDMs). Hypotonic stress increased endocytic activity in BMDMs, but there was no significant change in the expression of CD80, CD86, and MHC class II molecules, nor in the secretion of TNF-α or IL-10 by BMDMs. Furthermore, the enhanced endocytic activity of BMDMs triggered by hypotonic stress was significantly inhibited by chloride channel-3 (ClC-3) siRNA. Our findings suggest that hypotonic stress can induce endocytosis in BMDMs and that ClC-3 plays a central role in the endocytic process.
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 4340030, China.
Yutao
Y
Yan
Department of Immunology, Tongji Medical College.
Yu
Y
Ding
Bingxia
B
Ming
Wenjiao
W
Du
Xiaoling
X
Kong
Li
L
Tian
Fang
F
Zheng
Min
M
Fang
Zheng
Z
Tan
Feili
F
Gong
D000818:Animals; D017209:Apoptosis; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D002478:Cells, Cultured; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D016753:Interleukin-10 / Q000378:metabolism; D008264:Macrophages / Q000502:physiology*; D008810:Mice, Inbred C57BL; D009997:Osmotic Pressure; D010873:Pinocytosis; D013312:Stress, Physiological; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D018118:Chloride Channels; C115376:ClC-3 channel; C556062:IL10 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10