Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on H2O2-induced C2C12 cells.
The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of these peptides were determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+•) radical scavenging assays. The in vitro cytotoxicity of T.peptide and MPG peptide was determined against H2O2-induced C2C12 cells. H2O2-induced apoptosis in C2C12 cells were determined by mRNA expression of caspase-3. Moreover, the mRNA expression of tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-κB) were assayed by reverse transcription polymerase chain reaction. The findings of the study indicate that the mRNA expression of TNF-α and NF-κB are significantly (p < 0.05) increased in H2O2-induced C2C12 cells, whereas a significant decrease (p < 0.05) in these mRNA expressions are observed when pretreated with T.peptide or MPG peptide. Pretreatment with MPG or T.peptides is also found to significantly (p < 0.05) decrease the mRNA expression of caspase-3 in H2O2-induced C2C12 cells. The results of the study demonstrate that both T.peptide and MPG peptide could reduce the DPPH• and ABTS+• radical and inhibit cytotoxicity against H2O2-induced injury, resulting in prevention of free radical generation and subsequent apoptotic cell death, which indicates the potential of bovine meat as a source of antioxidant peptides.
Department of Animal Science and BK21 PLUS program, Chonbuk National University, Jeonju, 561-756, South Korea.
Allur Subramaniyan
AS
Sivakumar
Chinzorio
C
Ochirbat
Soo-Hyun
SH
Cho
Jieun
J
Yang
Inho
I
Hwang
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D053148:Caspase 3 / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004268:DNA-Binding Proteins / Q000494:pharmacology*; D006861:Hydrogen Peroxide; D051379:Mice; D018482:Muscle, Skeletal / Q000737:chemistry*; D016328:NF-kappa B / Q000378:metabolism; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D010851:Picrates; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013451:Sulfonic Acids; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D004268:DNA-Binding Proteins; C475074:MPG peptide; D016328:NF-kappa B; D010455:Peptides; D010851:Picrates; D013451:Sulfonic Acids; D014409:Tumor Necrosis Factor-alpha; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D053148:Caspase 3