Cryo-scanning electron microscopy to study the freezing behavior of plant tissues.
A cryo-scanning electron microscope (cryo-SEM) is a valuable tool for observing bulk frozen samples to monitor freezing responses of plant tissues and cells. Here, essential processes of a cryo-SEM to observe freezing behaviors of plant tissue cells are described.
Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-8589, Japan, sfuji@for.agr.hokudai.ac.jp.
Seizo
S
Fujikawa
Keita
K
Endoh
D002473:Cell Wall / Q000737:chemistry; D020285:Cryoelectron Microscopy / Q000379:methods*; D005110:Extracellular Space / Q000737:chemistry; D005615:Freezing; D007053:Ice; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D010944:Plants / Q000737:chemistry* / Q000648:ultrastructure*; D016707:Tissue Fixation
D007053:Ice