[Establishment of fluorescence labeling and capillary electrophoresis in MSAP for Beijing You chicken].
The DNA methylated states in muscle tissues from Beijing You chicken were analyzed using methylation sensitive amplified polymorphism (MSAP) combined with fluorescence labeling and capillary electrophoresis technology. We optimized the MSAP experimental condition including the genomic DNA concentration, dilution of pre-amplification prod-uct, the internal standard content and the concentrations of selective primers, Mg2+ and dNTPs. Repeated experiments showed that this method can automatically detect the global DNA methylation states in Beijing You chicken, and can be extended to other animals or plants with complex genomes and rich methylation polymorphism.
Institute of Life Science and Biotechnology, Beijing Jiaotong University, Beijing 100044, China.
Li
L
Jinlong
Beijing Animal Husbandry Station, Beijing 100029, China.
Tang
T
Shaoqing
Institute of Software, Beijing Jiaotong University, Beijing 100044, China.
Zou
Z
Zhiyuan
Institute of Life Science and Biotechnology, Beijing Jiaotong University, Beijing 100044, China.
Wang
W
Haichao
Institute of Life Science and Biotechnology, Beijing Jiaotong University, Beijing 100044, China.
Xu
X
Qing
D000818:Animals; D002645:Chickens / Q000235:genetics*; D019175:DNA Methylation; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D023281:Genomics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D013194:Staining and Labeling
D005456:Fluorescent Dyes