Effect of captopril on collagen metabolisms in keloid fibroblast cells.
BACKGROUND
Keloid is a proliferative disease of fibrous tissues. The mechanism and consistently effective treatments of keloid remained unknown. Although there was a report about treating keloid with topical captopril, the further investigation about captopril affecting keloid has not been performed so far.


OBJECTIVE
The aim of this study was to analyse the effect of captopril on collagen metabolisms in keloid fibroblast cells, and to provide information for the mechanism and therapy of keloid.


METHODS
To investigate the effects and relative mechanism of captopril on keloid fibroblast cells, we examined the changes of collagen metabolism, expression of angiotensin, transforming growth factor (TGF)-β1, platelet-derived growth factor (PDGF)-BB and heat shock protein 47 (HSP47), and cellular proliferation in keloid fibroblast cells.


RESULTS
We found that all collagen metabolisms, expression of TGF-β1, PDGF-BB and HSP47, and cellular proliferation decreased significantly with effective captopril concentrations in keloid fibroblast cells.


CONCLUSIONS
With a comprehensive analysis of test results, we proposed that captopril may decrease the expression of angiotensin, PDGF-BB, TGF-β1 and HSP47, and further inhibit proliferation and collagen synthesis of keloid fibroblast cells, which were the key in keloid formation.
Department of Burns and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Junjie
J
Chen
Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Sha
S
Zhao
Department of Burns and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Yong
Y
Liu
Department of Burns and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Ying
Y
Cen
Department of Burns and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Crook
C
Nicolas
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D015153:Blotting, Western; D002216:Captopril / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007627:Keloid / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012313:RNA / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
D000806:Angiotensin-Converting Enzyme Inhibitors; D012313:RNA; D003094:Collagen; D002216:Captopril

HSP47; captopril; collagen metabolism; keloid