Structural and biocompatibility properties of dextran from Weissella cibaria JAG8 as food additive.
Dextran produced from Weissella cibaria JAG8 was purified and characterized. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive.
Department of Biotechnology, Indian Institute of Technology Guwahati , Guwahati, Assam , India.
Jagan Mohan Rao
JM
Tingirikari
Damini
D
Kothari
Rishikesh
R
Shukla
Arun
A
Goyal
D000818:Animals; D003911:Dextrans / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D055050:Diet, Gluten-Free; D005503:Food Additives; D005947:Glucose / Q000032:analysis; D005964:Glucosyltransferases / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D015416:Maillard Reaction; D008892:Milk / Q000382:microbiology*; D008970:Molecular Weight; D056692:Prebiotics; D013395:Sucrose / Q000378:metabolism; D058836:Weissella / Q000378:metabolism*
D003911:Dextrans; D005503:Food Additives; D056692:Prebiotics; D013395:Sucrose; D005964:Glucosyltransferases; C020025:dextransucrase; D005947:Glucose
Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk