Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein.
Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis.
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02129, USA. sikes@mit.edu.
Brandon W
BW
Heimer
Tatyana A
TA
Shatova
Jungkyu K
JK
Lee
Kaja
K
Kaastrup
Hadley D
HD
Sikes
D000818:Animals; D018899:CpG Islands; D019175:DNA Methylation; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D004867:Equipment Design; D006801:Humans; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic
D004268:DNA-Binding Proteins; C489323:Mbd1 protein, mouse