Role of leptin and ghrelin in induction of differentiation of IL-17-producing and T-regulatory cells.
We studied isolated and combined effects of leptin and ghrelin on the formation of IL-producing and T-regulatory cells. Leptin in concentrations comparable with its normal blood concentration during pregnancy (trimester II-III) promotes differentiation of peripheral blood CD4(+) cells to IL-17-producing cells and enhances IL-17A production, but suppresses the formation of T-regulatory cells in vitro. In contrast, ghrelin in a concentration typical of trimester I-II of pregnancy reduces the number of IL-17-producing cells, but stimulated the formation of T-regulatory cells. The effects of leptin and ghrelin in combination typical of trimester I-II of pregnancy stimulated the formation of T-regulatory cells, while in combination typical of trimester II-III did not shift the balance of T-regulatory cells and IL-17-producing cells, but stimulated the formation of IL-17A. We conclude that leptin and ghrelin play an important role in the maintenance of the balance of IL-17-producing and T-regulatory cells during pregnancy.
Laboratory of Immunoregulation, Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences, Perm', Russia, orlova_katy@mail.ru.
E G
EG
Orlova
S V
SV
Shirshev
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D005260:Female; D005434:Flow Cytometry; D005453:Fluorescence; D051858:Forkhead Transcription Factors / Q000378:metabolism; D054439:Ghrelin / Q000494:pharmacology*; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D020738:Leptin / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear; D011247:Pregnancy; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000502:physiology*
C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D054439:Ghrelin; D020381:Interleukin-17; D020738:Leptin