Proteomic analysis of the human skin proteome after in vivo treatment with sodium dodecyl sulphate.
BACKGROUND
Skin has a variety of functions that are incompletely understood at the molecular level. As the most accessible tissue in the body it often reveals the first signs of inflammation or infection and also represents a potentially valuable source of biomarkers for several diseases. In this study we surveyed the skin proteome qualitatively using gel electrophoresis, liquid chromatography tandem mass spectrometry (GeLC-MS/MS) and quantitatively using an isobaric tagging strategy (iTRAQ) to characterise the response of human skin following exposure to sodium dodecyl sulphate (SDS).


RESULTS
A total of 653 skin proteins were assigned, 159 of which were identified using GeLC-MS/MS and 616 using iTRAQ, representing the most comprehensive proteomic study in human skin tissue. Statistical analysis of the available iTRAQ data did not reveal any significant differences in the measured skin proteome after 4 hours exposure to the model irritant SDS.


CONCLUSIONS
This study represents the first step in defining the critical response to an irritant at the level of the proteome and provides a valuable resource for further studies at the later stages of irritant exposure.
Centre for Biological Sciences, University of Southampton, Southampton, United Kingdom; Centre for Proteomic Research, Institute for Life Sciences, University of Southampton, Southampton, United Kingdom.
Erika
E
Parkinson
Centre for Biological Sciences, University of Southampton, Southampton, United Kingdom; Centre for Proteomic Research, Institute for Life Sciences, University of Southampton, Southampton, United Kingdom.
Paul
P
Skipp
Safety & Environmental Assurance Centre, Unilever, Colworth Science Park, Sharnbrook, United Kingdom.
Maja
M
Aleksic
Safety & Environmental Assurance Centre, Unilever, Colworth Science Park, Sharnbrook, United Kingdom.
Andrew
A
Garrow
Unilever Clinicals, Unilever Research, Colworth Science Park, Sharnbrook, United Kingdom.
Tony
T
Dadd
Safety & Environmental Assurance Centre, Unilever, Colworth Science Park, Sharnbrook, United Kingdom.
Michael
M
Hughes
Institute for Developmental Sciences, School of Medicine, University of Southampton, Southampton, United Kingdom.
Geraldine
G
Clough
Centre for Biological Sciences, University of Southampton, Southampton, United Kingdom; Centre for Proteomic Research, Institute for Life Sciences, University of Southampton, Southampton, United Kingdom.
C David
CD
O'Connor
D006801:Humans; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D012995:Solubility
D020543:Proteome; D012967:Sodium Dodecyl Sulfate