Border control--a membrane-linked interactome of Arabidopsis.
Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 10(6) pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis. Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, >99% of the interactions were previously unknown. Interactions were confirmed at a rate of 32% in orthogonal in planta split-green flourescent protein interaction assays, which was statistically indistinguishable from the confirmation rate for known interactions collected from literature (38%). Regulatory associations in membrane protein trafficking, turnover, and phosphorylation include regulation of potassium channel activity through abscisic acid signaling, transporter activity by a WNK kinase, and a brassinolide receptor kinase by trafficking-related proteins. These examples underscore the utility of the membrane/signaling protein interaction network for gene discovery and hypothesis generation in plants and other organisms.
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Alexander M
AM
Jones
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Yuanhu
Y
Xuan
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Meng
M
Xu
Department of Physics, Pennsylvania State University, University Park, PA 16802, USA.
Rui-Sheng
RS
Wang
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Cheng-Hsun
CH
Ho
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Sylvie
S
Lalonde
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Chang Hun
CH
You
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Maria I
MI
Sardi
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Saman A
SA
Parsa
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Erika
E
Smith-Valle
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Tianying
T
Su
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Keith A
KA
Frazer
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA. Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic University and State University, Blacksburg, VA 24061, USA.
Guillaume
G
Pilot
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA. Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic University and State University, Blacksburg, VA 24061, USA.
Réjane
R
Pratelli
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA.
Guido
G
Grossmann
Department of Biology, Pennsylvania State University, University Park, PA 16802, USA.
Biswa R
BR
Acharya
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
Heng-Cheng
HC
Hu
Cell and Developmental Biology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
Cawas
C
Engineer
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
Florent
F
Villiers
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
Chuanli
C
Ju
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
Kouji
K
Takeda
Department of Biology, Pennsylvania State University, University Park, PA 16802, USA.
Zhao
Z
Su
Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA.
Qunfeng
Q
Dong
Department of Biology, Pennsylvania State University, University Park, PA 16802, USA.
Sarah M
SM
Assmann
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA. Michigan State University-U.S. Department of Energy (MSU-DOE) Plant Research Laboratory and Department of Computer Science and Engineering, Michigan State University, East Lansing, MI 48824, USA.
Jin
J
Chen
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA. Center for Plant Aging Research, Institute for Basic Science, Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology, Daegu 711-873, Republic of Korea.
June M
JM
Kwak
Cell and Developmental Biology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
Julian I
JI
Schroeder
Department of Physics, Pennsylvania State University, University Park, PA 16802, USA.
Reka
R
Albert
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA. wfrommer@stanford.edu srhee@carnegiescience.edu.
Seung Y
SY
Rhee
Department of Plant Biology, Carnegie Institution for Science, CA 94305, USA. wfrommer@stanford.edu srhee@carnegiescience.edu.
Wolf B
WB
Frommer
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D060066:Protein Interaction Maps; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
D029681:Arabidopsis Proteins; D008565:Membrane Proteins