Aspergillus fumigatus induces microRNA-132 in human monocytes and dendritic cells.
Aspergillus fumigatus is responsible for severe and often fatal infections in immunocompromised patients. The human immune response against this pathogenic mould is still not fully understood. Recently, microRNAs (miRNAs) have been characterized as regulators of inflammation and immune response in various diseases. MiRNAs specifically bind to mRNA target sequences, thereby leading to gene silencing by target degradation and/or translational repression. To investigate the possible role of miRNAs during A. fumigatus infection, we studied the expression of two major immune relevant miRNAs, miR-132 and miR-155, in human monocytes and dendritic cells (DCs). Both cell types are crucial for the immune response against A. fumigatus. Here, we demonstrate for the first time that miR-132 and miR-155 are differentially expressed in monocytes and DCs upon stimulation with A. fumigatus or bacterial lipopolysaccharide (LPS). Interestingly, miR-132 was induced by A. fumigatus but not by LPS in both cell types. Our data suggest that miR-132 may be a relevant regulator of the immune response directed against A. fumigatus.
University Hospital Wuerzburg, Medical Clinic and Polyclinic II, Wuerzburg, Germany.
Mithun
M
Das Gupta
University Hospital Wuerzburg, Medical Clinic and Polyclinic II, Wuerzburg, Germany.
Mirjam
M
Fliesser
University Hospital Wuerzburg, Medical Clinic and Polyclinic II, Wuerzburg, Germany.
Jan
J
Springer
University Hospital Wuerzburg, Medical Clinic and Polyclinic II, Wuerzburg, Germany.
Tanja
T
Breitschopf
University Hospital Wuerzburg, Medical Clinic and Polyclinic II, Wuerzburg, Germany.
Hannes
H
Schlossnagel
University Hospital Wuerzburg, Medical Clinic and Polyclinic II, Wuerzburg, Germany.
Anna-Lena
AL
Schmitt
Septomics Research Centre, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology-Hans Knoell Institute, Jena, Germany.
Oliver
O
Kurzai
Septomics Research Centre, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology-Hans Knoell Institute, Jena, Germany.
Kerstin
K
Hünniger
University Hospital Wuerzburg, Medical Clinic and Polyclinic II, Wuerzburg, Germany.
Hermann
H
Einsele
University Hospital Wuerzburg, Medical Clinic and Polyclinic II, Wuerzburg, Germany. Electronic address: loeffler_j@ukw.de.
Jürgen
J
Löffler
D001232:Aspergillus fumigatus / Q000254:growth & development* / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D009000:Monocytes / Q000276:immunology / Q000382:microbiology*; D015533:Transcriptional Activation
C547077:MIRN132 microRNA, human; C524942:MIRN155 microRNA, human; D035683:MicroRNAs
Aspergillus fumigatus; Dendritic cells; Microrna; Monocytes