Phosphoproteome profiles of the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea during exponential growth in axenic cultures.
This study describes the gel-free phosphoproteomic analysis of the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea grown in vitro under nonlimiting conditions. Using a combination of strong cation exchange and IMAC prior to LC-MS, we identified over 1350 phosphopeptides per fungus representing over 800 phosphoproteins. The preferred phosphorylation sites were found on serine (>80%) and threonine (>15%), whereas phosphorylated tyrosine residues were found at less than 1% in A. brassicicola and at a slightly higher ratio in B. cinerea (1.5%). Biological processes represented principally among the phoshoproteins were those involved in response and transduction of stimuli as well as in regulation of cellular and metabolic processes. Most known elements of signal transduction were found in the datasets of both fungi. This study also revealed unexpected phosphorylation sites in histidine kinases, a category overrepresented in filamentous ascomycetes compared to yeast. The data have been deposited to the ProteomeXchange database with identifier PXD000817 (http://proteomecentral.proteomexchange.org/dataset/PXD000817).
CNRS, Plateforme d'Analyse Protéomique de Paris Sud Ouest, PAPPSO, Gif-sur-Yvette, France; INRA/University Paris-Sud/CNRS/AgroParisTech, UMR 0320/UMR 8120 Génétique Végétale, Gif-sur-Yvette, France.
Marlène
M
Davanture
Jérôme
J
Dumur
Nelly
N
Bataillé-Simoneau
Claire
C
Campion
Benoît
B
Valot
Michel
M
Zivy
Philippe
P
Simoneau
Sabine
S
Fillinger
D000528:Alternaria / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D000595:Amino Acid Sequence; D061245:Axenic Culture; D020171:Botrytis / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction
D005656:Fungal Proteins; D010750:Phosphoproteins
Black spot; Gray mold; Microbiology; PTMs; Protein phosphorylation; Signal transduction