Synthetic disialyl hexasaccharides protect neonatal rats from necrotizing enterocolitis.
Two novel synthetic α2-6-linked disialyl hexasaccharides, disialyllacto-N-neotetraose (DSLNnT) and α2-6-linked disialyllacto-N-tetraose (DS'LNT), were readily obtained by highly efficient one-pot multienzyme (OPME) reactions. The sequential OPME systems described herein allowed the use of an inexpensive disaccharide and simple monosaccharides to synthesize the desired complex oligosaccharides with high efficiency and selectivity. DSLNnT and DS'LNT were shown to protect neonatal rats from necrotizing enterocolitis (NEC) and are good therapeutic candidates for preclinical experiments and clinical application in treating NEC in preterm infants.
Department of Chemistry, University of California-Davis, One Shields Avenue, Davis, CA 95616 (USA).
Hai
H
Yu
Kam
K
Lau
Vireak
V
Thon
Chloe A
CA
Autran
Evelyn
E
Jantscher-Krenn
Mengyang
M
Xue
Yanhong
Y
Li
Go
G
Sugiarto
Jingyao
J
Qu
Shengmao
S
Mu
Li
L
Ding
Lars
L
Bode
Xi
X
Chen
D000818:Animals; D001644:Bifidobacterium / Q000201:enzymology; D004353:Drug Evaluation, Preclinical; D020345:Enterocolitis, Necrotizing / Q000188:drug therapy*; D009097:Multienzyme Complexes / Q000378:metabolism; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000627:therapeutic use; D020011:Protective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats
D009097:Multienzyme Complexes; D009844:Oligosaccharides; D020011:Protective Agents; C576246:disialyllacto-N-tetraose
disialyl glycans; enzymatic synthesis; enzymes; necrotizing enterocolitis; oligosaccharides