Wogonoside ameliorates lipopolysaccharide-induced acute lung injury in mice.
Wogonoside has been reported to have anti-inflammatory properties. In this study, we evaluated the effect of wogonoside on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Male BALB/c mice with ALI, induced by intranasal instillation of LPS, were treated with wogonoside 1 h prior to LPS exposure. Mice treated with LPS alone showed significantly increased TNF-α, IL-6, and IL-1β levels in the bronchoalveolar lavage fluid (BALF). When pretreated with wogonoside, the TNF-α, IL-6, and IL-1β levels were significantly decreased. Meanwhile, wogonoside significantly inhibited LPS-induced increases in the macrophage and neutrophil infiltration of lung tissues and markedly attenuated myeloperoxidase activity. Furthermore, wogonoside inhibited the TLR4 expression and the phosphorylation of NF-κB p65, and IκB induced by LPS. In conclusion, our results indicate that wogonoside exhibits a protective effect on LPS-induced ALI via suppression of TLR4-mediated NF-κB signaling pathways.
Department of Thoracic Surgery, Liaoning Cancer Hospital and Institute, Shenyang, 110042, Liaoning Province, People's Republic of China.
Liang
L
Zhang
Yi
Y
Ren
Chengliang
C
Yang
Yue
Y
Guo
Xiaojing
X
Zhang
Gang
G
Hou
Xinjin
X
Guo
Nan
N
Sun
Yongyu
Y
Liu
D055371:Acute Lung Injury / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D004305:Dose-Response Relationship, Drug; D044950:Flavanones / Q000627:therapeutic use*; D005960:Glucosides / Q000627:therapeutic use*; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D044950:Flavanones; D005960:Glucosides; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; C473995:wogonoside