A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis.
The sesquiterpenoid abscisic acid (ABA) mediates an assortment of responses across a variety of kingdoms including both higher plants and animals. In plants, where most is known, a linear core ABA signaling pathway has been identified. However, the complexity of ABA-dependent gene expression suggests that ABA functions through an intricate network. Here, using systems biology approaches that focused on genes transcriptionally regulated by ABA, we defined an ABA signaling network of over 500 interactions among 138 proteins. This map greatly expanded ABA core signaling but was still manageable for systematic analysis. For example, functional analysis was used to identify an ABA module centered on two sucrose nonfermenting (SNF)-like kinases. We also used coexpression analysis of interacting partners within the network to uncover dynamic subnetwork structures in response to different abiotic stresses. This comprehensive ABA resource allows for application of approaches to understanding ABA functions in higher plants.
Cell & Systems Biology, University of Toronto and the Centre for The Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON M5S 3B2, Canada.
Shelley
S
Lumba
Cell & Systems Biology, University of Toronto and the Centre for The Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON M5S 3B2, Canada.
Shigeo
S
Toh
Department of Computer Science, University of Toronto, Toronto, ON M5S 2E4, Canada.
Louis-François
LF
Handfield
Cell & Systems Biology, University of Toronto and the Centre for The Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON M5S 3B2, Canada.
Michael
M
Swan
Cell & Systems Biology, University of Toronto and the Centre for The Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON M5S 3B2, Canada.
Raymond
R
Liu
Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.
Ji-Young
JY
Youn
Botany and Plant Sciences, Chemistry Genomics Building, University of California, Riverside, Riverside, CA 92521, USA.
Sean R
SR
Cutler
Agriculture and Agrifood Canada, 960 Carling Avenue, Ottawa, ON K1A 06C, Canada.
Rajagopal
R
Subramaniam
Cell & Systems Biology, University of Toronto and the Centre for The Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON M5S 3B2, Canada.
Nicholas
N
Provart
Cell & Systems Biology, University of Toronto and the Centre for The Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON M5S 3B2, Canada; Department of Computer Science, University of Toronto, Toronto, ON M5S 2E4, Canada.
Alan
A
Moses
Cell & Systems Biology, University of Toronto and the Centre for The Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON M5S 3B2, Canada. Electronic address: darrell.desveaux@utoronto.ca.
Darrell
D
Desveaux
Cell & Systems Biology, University of Toronto and the Centre for The Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON M5S 3B2, Canada. Electronic address: peter.mccourt@utoronto.ca.
Peter
P
McCourt
D000040:Abscisic Acid / Q000494:pharmacology / Q000502:physiology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000235:genetics; D060066:Protein Interaction Maps; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics
D029681:Arabidopsis Proteins; D000040:Abscisic Acid; D017346:Protein Serine-Threonine Kinases