Influence of the level of arsenic (As) exposure and the presence of T860C polymorphism in human As urinary metabolic profile.
The influence, on arsenic (As) urinary metabolic profile, of the level of As exposure was evaluated on chronic-exposed inhabitants of several locations of the Chaco-Pampean Plains in Argentina. Urinary As (UAs) was quantified as a measure of the level of exposure. The metabolic profile of UAs (inorganic As, monomethylarsonic acid, and dimethylarsinic acid) was also evaluated. The presence of T860C polymorphism on the arsenite methyltransferase encoding gene was investigated by desquamation of buccal cells. UAs showed a wide range of levels (from 18 µg/g to 4103 µg/g) of creatinine. A clear influence of age, gender, level of As exposure, and the presence of T860C polymorphism was observed on As metabolic profile. The influence of the level of exposure showed to be different between individuals carrying the wild type (WT) and the heterozygous (H) genotypes. Metabolic profile of individuals carrying the WT genotype seemed to be influenced by the level of exposure, while individuals with the H genotype did not. It is concluded that the level of As exposure seemed to have a significant influence on urinary metabolic profile of individuals carrying the WT genotype. In contrast, individuals carrying the H genotype seemed not to be affected the same way by increasing the As exposure level.
Cátedra de Toxicología y Química Legal, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina volmos@ffyb.uba.ar.
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V
Olmos
Cátedra de Toxicología y Química Legal, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
J A
JA
Navoni
Cátedra de Matemáticas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
M L
ML
Calcagno
Cátedra de Toxicología y Química Legal, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
A H
AH
Sassone
Cátedra de Toxicología y Química Legal, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
E C
EC
Villaamil Lepori
D000293:Adolescent; D000328:Adult; D001118:Argentina; D001151:Arsenic / Q000032:analysis / Q000652:urine*; D001152:Arsenicals / Q000652:urine; D002101:Cacodylic Acid / Q000652:urine; D002648:Child; D060766:Drinking Water / Q000032:analysis; D004784:Environmental Monitoring; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008780:Methyltransferases / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000652:urine*; D055815:Young Adult
D001152:Arsenicals; D060766:Drinking Water; D014874:Water Pollutants, Chemical; D002101:Cacodylic Acid; D008780:Methyltransferases; C505756:AS3MT protein, human; C020300:monomethylarsonic acid; D001151:Arsenic
Arsenic; influencing factors; level of exposure; metabolic profile; metabolism; polymorphism