Association of rs8066560 variant in the sterol regulatory element-binding protein 1 (SREBP-1) and miR-33b genes with hyperglycemia and insulin resistance.
OBJECTIVE
This study aims to evaluate the association of miR-33b/SREBP-1 rs8066560 with hyperglycemia and insulin resistance in Iranian children and adolescents.


METHODS
This case-control study comprised 200 participants with 9-19 years of age, consisting of 100 MetS and 100 healthy controls. Anthropometric and biochemical parameters were measured, and the polymorphism was genotyped by TaqMan real-time PCR method.


RESULTS
Logistic regression analysis of genotype distribution of rs8066560 did not show a significant association with MetS neither in boys (OR, 1.745; 95% CI, 0.695-4.382; p=0.236) nor in girls (OR, 1.075; 95% CI, 0.420-2.752; p=0.880). Participants with GG/AG genotypes had significant higher LDL-C levels than those with the AA genotype. No statistically significant difference was documented in fasting blood glucose and insulin levels between the three rs8066560 genotypes.


CONCLUSIONS
We found that rs8066560 variant on SREBP-1 and miR-33b genes is associated with LDL-C levels; however, it may not be an important determinant for the MetS, hyperglycemia, and insulin resistance in children and adolescents.
Parvaneh
P
Nikpour
Hajar
H
Miranzadeh-Mahabadi
Modjtaba
M
Emadi-Baygi
Roya
R
Kelishadi
D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D008078:Cholesterol, LDL / Q000378:metabolism; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D006943:Hyperglycemia / Q000453:epidemiology / Q000235:genetics*; D007333:Insulin Resistance / Q000235:genetics*; D007492:Iran / Q000453:epidemiology; D008297:Male; D024821:Metabolic Syndrome / Q000453:epidemiology / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics*; D055815:Young Adult
D008078:Cholesterol, LDL; C550416:MIRN33a microRNA, human; D035683:MicroRNAs; C497387:SREBF1 protein, human; D051780:Sterol Regulatory Element Binding Protein 1