Metabonomics based NMR in Crohn's disease applying PLS-DA.
OBJECTIVE
The aim of this study was to search for metabolic biomarkers of Crohn's disease (CD).


BACKGROUND
Crohn's disease (CD) is a type of inflammatory bowel disease that causing a wide variety of symptoms. CD can influence any part of the gastrointestinal tract from mouth to anus. CD is not easily diagnosed because monitoring tools are currently insufficient. Thus, the discovery of proper methods is needed for early diagnosis of CD.


METHODS
We utilized metabolic profiling using proton nuclear magnetic resonance spectroscopy ((1)HNMR) to find the metabolites in serum. Classification of CD and healthy subject was done using partial least squares discriminant analysis (PLS-DA).


RESULTS
According to PLS-DA model, we concluded that just using one descriptor CD and control groups could be classified separately. The level of lipid in blood serum of CD compared to healthy cohorts was decreased. For the external test set, the classification model showed a 94% correct classification of CD and healthy subject.


CONCLUSIONS
The result of classification model presents that NMR based metabonomics is key tool as well as insight into potential targets for disease therapy and prevention.
Department of Chemistry, Sharif University of Technology, Tehran, Iran.
Fariba
F
Fathi
Department of Basic Science, Faculty of Paramedical, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Afsaneh Arefi
AA
Oskouie
Department of Chemistry, Sharif University of Technology, Tehran, Iran.
Mohsen
M
Tafazzoli
Gastroenterology and Liver Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Nosratollah
N
Naderi
Department of electrical engineer, Golpayegan Payam University, Tehran, Iran.
Kaveh
K
Sohrabzedeh
Department of Mathematics, Teacher Training University, Tehran, Iran.
Soraya
S
Fathi
Gastroenterology and Liver Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohsen
M
Norouzinia
Gastroenterology and Liver Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammad
M
Rostami Nejad
1HNMR; Crohn's diseasel; Metabonomics; PLS-DA