A novel method for the rapid detection of benzo(a)pyrene in liquid milk by dimethyl sulfoxide selectively enhanced synchronous fluorescence spectrometry.
Based on the high solubility efficiency and strong fluorescence response of benzo(a)pyrene (BaP) in dimethyl sulfoxide in combination with the high-performance derivative constant-energy synchronous fluorescence spectroscopic (DCESFS) technique, a simple, sensitive and economic method was developed for the determination of BaP in liquid milk. This method comprises ultrasound-assisted solvent extraction, solvent replacement and DCESFS detection. No saponification or other tedious clean-up procedures were needed. The recoveries of BaP in different milk samples were greater than 82%. Detection limits in full- and low-fat milk were 0.03 and 0.04 μg kg(-1), respectively.
a Department of Chemistry and MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering , Xiamen University , Xiamen , China.
Li-Rong
LR
Lin
He-Dong
HD
Luo
Xiu-Ying
XY
Li
Na
N
Li
Na
N
Zhou
Yu-Zhu
YZ
Jia
Yi-Hong
YH
Liu
Yao-Qun
YQ
Li
D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis* / Q000633:toxicity; D004121:Dimethyl Sulfoxide; D004785:Environmental Pollutants / Q000032:analysis / Q000633:toxicity; D005449:Fluorenes / Q000032:analysis / Q000633:toxicity; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry* / Q000633:toxicity; D012997:Solvents; D013053:Spectrophotometry / Q000379:methods*
D004785:Environmental Pollutants; D005449:Fluorenes; D012997:Solvents; D001564:Benzo(a)pyrene; C022921:benzo(k)fluoranthene; D004121:Dimethyl Sulfoxide
benzo(a)pyrene; constant-energy synchronous fluorescence spectra; dimethyl sulfoxide; milk