Non-microfluidic methods for imaging live C. elegans.
There are many challenges to live Caenorhabditis elegans imaging including the high motility of the animals and sustaining their viability for extended periods of time. Commonly used anesthetics to immobilize the C. elegans for imaging purpose prevents feeding of the animals and can cause cellular physiologic changes. Here we present three adapted or novel methodologies to image live C. elegans over different imaging microscopy equipment to allow for visualization of animals by DIC and fluorescence without the use of microfluidic technologies. The methods present here use common microscopy consumables and equipment found in many imaging core facilities and can be easily adapted to fit on multiple microscopy systems.
Department of Pediatrics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh, One Children's Hospital Drive, 4401 Penn Avenue, Pittsburgh, PA 15224, USA. Electronic address: cliff.luke@chp.edu.
Cliff J
CJ
Luke
Department of Pediatrics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh, One Children's Hospital Drive, 4401 Penn Avenue, Pittsburgh, PA 15224, USA.
Jason Z
JZ
Niehaus
Department of Pediatrics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh, One Children's Hospital Drive, 4401 Penn Avenue, Pittsburgh, PA 15224, USA.
Linda P
LP
O'Reilly
Department of Cell Biology and Physiology, Center for Biologic Imaging, University of Pittsburgh School of Medicine, 3500 Terrace Street, S233 BST, Pittsburgh, PA 15261, USA.
Simon C
SC
Watkins
D000818:Animals; D017173:Caenorhabditis elegans; D008856:Microscopy, Fluorescence / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*
Caenorhabditis elegans; Confocal; Fluorescence; Live-cell imaging; Microscopy; Widefield