A simple and safe technique for longitudinal bone marrow aspiration in cynomolgus and rhesus macaques.
Longitudinal bone marrow aspirates were obtained aseptically from the humerus of 36 rhesus and 6 cynomolgus macaques by using a 20G spinal needle, introduced through the bone close to the greater tuberosity. All samplings were performed without complications, and the animals showed no signs of pain or infections. The amount of total bone marrow cells obtained from each aspiration varied, in part due to animal-to-animal variation, but the yields were not affected by the sampling frequency or the length of time between each aspiration. The frequency of plasma cells in the bone marrow of each animal was also fairly stable over several longitudinal samplings while a greater, age-dependent, variation was observed between different animals.
Comparative Medicine, Astrid Fagraeus Laboratory, Karolinska Institutet, Stockholm, Sweden. Electronic address: mats.spangberg@ki.se.
Mats
M
Spångberg
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Paola
P
Martinez
Comparative Medicine, Astrid Fagraeus Laboratory, Karolinska Institutet, Stockholm, Sweden.
Helene
H
Fredlund
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Gunilla B
GB
Karlsson Hedestam
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden. Electronic address: christopher.sundling@ki.se.
Christopher
C
Sundling
D000367:Age Factors; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology*; D001707:Biopsy, Needle / Q000379:methods*; D001854:Bone Marrow Cells / Q000276:immunology / Q000502:physiology*; D001856:Bone Marrow Examination; D002469:Cell Separation; D006811:Humerus / Q000166:cytology*; D007074:Immunoglobulin G / Q000378:metabolism; D008252:Macaca fascicularis; D008253:Macaca mulatta; D010950:Plasma Cells / Q000276:immunology / Q000502:physiology*; D013396:Suction; D013997:Time Factors
D007074:Immunoglobulin G
B cell; Bone marrow aspirate; Macaque; Non-human primate; Plasma cell; Technique