Ethanol production from xylan-removed sugarcane bagasse using low loading of commercial cellulase.
Xylan was always extracted as the feedstock for xylooligosaccharides production. The xylan-removed residue may contain high content of cellulose and thus had a possibility to be converted into ethanol. After soaked in 12% of NaOH at room temperature overnight, solubilization of cellulose, xylan, and lignin was 4.64%, 72.06%, and 81.87% respectively. The xylan-removed sugarcane bagasse (XRSB) was enzymatically hydrolyzed by using decreased cellulase loadings. The results showed that 7.5 FPU/g cellulose could obtain a cellulose conversion yield of 82%. Increasing the cellulase loading did not result in higher yield. Based on this, bioethanol production was performed using 7.5 FPU/g cellulose by employing fed-batch fermentation mode. The final ethanol concentration reached 40.59 g/L corresponding to 74.2% of the theoretical maximum. The high titer ethanol and low cellulase loading may reduce the overall cost.
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China. Electronic address: jingbo_li@163.com.
Jingbo
J
Li
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Pengfei
P
Zhou
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Hongmei
H
Liu
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Kejing
K
Wu
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Wenjuan
W
Xiao
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Yingxue
Y
Gong
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Jianghai
J
Lin
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China. Electronic address: zhliu@jnu.edu.cn.
Zehuan
Z
Liu
D002480:Cellulase / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D006868:Hydrolysis; D031786:Saccharum / Q000378:metabolism*; D014990:Xylans / Q000302:isolation & purification* / Q000378:metabolism
D014990:Xylans; D000431:Ethanol; D002480:Cellulase
Bioethanol production; High solid loading; Low cellulase loading; NaOH extraction; Sugarcane bagasse