Human dermal fibroblast migration induced by fibronectin in autocrine and paracrine manners.
Although fibronectin (FN) is known as a chemoattractant for human dermal fibroblasts (HDFs), it is unclear whether HDF migration is stimulated by FN produced by HDFs (autocrine manner) or by keratinocytes (paracrine manner). In this study, we investigated HDF migration by Boyden chamber assay using conditioned media from HDFs and HaCaT cells (keratinocyte cell line). Immunoblotting and enzyme-linked immunosorbent assay revealed that FN existed in both conditioned media. Boyden chamber assay showed both conditioned media stimulated HDF migration, which was inhibited by anti-FN antibody. Antibodies to both integrin β1and β3 subunits inhibited HDF migration induced by HDF-conditioned medium almost completely and that by HaCaT cell-conditioned medium with 50-60%. These results suggested that HDF migration was stimulated by FN in both autocrine and paracrine manners. However, the mechanisms of HDF migration by FN, particularly the role of integrin β1 and β3 subunits, were slightly different between autocrine and paracrine manners.
Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, Kurume, Fukuoka, Japan.
Xiaoguang
X
Li
Hua
H
Qian
Fumitake
F
Ono
Atsunari
A
Tsuchisaka
Rafal P
RP
Krol
Koji
K
Ohara
Taihei
T
Hayakawa
Satoko
S
Matsueda
Tetsuro
T
Sasada
Chika
C
Ohata
Minao
M
Furumura
Takahiro
T
Hamada
Takashi
T
Hashimoto
D019898:Autocrine Communication; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000502:physiology*; D005353:Fibronectins / Q000502:physiology*; D006801:Humans; D019012:Integrin beta1 / Q000502:physiology; D039661:Integrin beta3 / Q000502:physiology; D015603:Keratinocytes / Q000502:physiology; D019899:Paracrine Communication; D012867:Skin / Q000166:cytology*; D012879:Skin Physiological Phenomena
D017077:Culture Media, Conditioned; D005353:Fibronectins; D019012:Integrin beta1; D039661:Integrin beta3
cell migration; fibronectin; human dermal fibroblasts; integrin