Brassica oleracea MATE encodes a citrate transporter and enhances aluminum tolerance in Arabidopsis thaliana.
The secretion of organic acid anions from roots is an important mechanism for plant aluminum (Al) tolerance. Here we report cloning and characterizing BoMATE (KF031944), a multidrug and toxic compound extrusion (MATE) family gene from cabbage (Brassica oleracea). The expression of BoMATE was more abundant in roots than in shoots, and it was highly induced by Al treatment. The (14)C-citrate efflux experiments in oocytes demonstrated that BoMATE is a citrate transporter. Electrophysiological analysis and SIET analysis of Xenopus oocytes expressing BoMATE indicated BoMATE is activated by Al. Transient expression of BoMATE in onion epidermal cells demonstrated that it localized to the plasma membrane. Compared with the wild-type Arabidopsis, the transgenic lines constitutively overexpressing BoMATE enhanced Al tolerance and increased citrate secretion. In addition, Arabidopsis transgenic lines had a lower K(+) efflux and higher H(+) efflux, in the presence of Al, than control wild type in the distal elongation zone (DEZ). This is the first direct evidence that MATE protein is involved in the K(+) and H(+) flux in response to Al treatment. Taken together, our results show that BoMATE is an Al-induced citrate transporter and enhances aluminum tolerance in Arabidopsis thaliana.
College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, People's Republic of ChinaThe first two authors contributed equally to this work.
Xinxin
X
Wu
College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, People's Republic of ChinaThe first two authors contributed equally to this work.
Ren
R
Li
College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, People's Republic of ChinaThe first two authors contributed equally to this work.
Jin
J
Shi
College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, People's Republic of ChinaThe first two authors contributed equally to this work.
Jinfang
J
Wang
College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, People's Republic of ChinaThe first two authors contributed equally to this work.
Qianqian
Q
Sun
College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, People's Republic of ChinaThe first two authors contributed equally to this work.
Haijun
H
Zhang
College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, People's Republic of ChinaThe first two authors contributed equally to this work.
Yanxia
Y
Xing
College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, People's Republic of ChinaThe first two authors contributed equally to this work.
Yan
Y
Qi
College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, People's Republic of ChinaThe first two authors contributed equally to this work.
Na
N
Zhang
College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, People's Republic of ChinaThe first two authors contributed equally to this work. yaguo@cau.edu.cn.
Yang-Dong
YD
Guo
D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D001692:Biological Transport; D001937:Brassica / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D019343:Citric Acid / Q000378:metabolism; D005260:Female; D018506:Gene Expression Regulation, Plant; D006859:Hydrogen / Q000378:metabolism; D008969:Molecular Sequence Data; D019697:Onions / Q000235:genetics / Q000502:physiology; D019441:Plant Epidermis / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D018520:Plant Shoots / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011188:Potassium / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological; D014981:Xenopus
D002352:Carrier Proteins; D010940:Plant Proteins; C020723:citrate-binding transport protein; D019343:Citric Acid; D006859:Hydrogen; D000535:Aluminum; D011188:Potassium
BoMATE; H+ flux; K+ flux; aluminum tolerance; cabbage; citrate