Application of ionic liquid-based dispersive liquid-liquid microextraction for the analysis of ochratoxin A in rice wines.
A novel and rapid ionic liquid-based dispersive liquid-liquid microextraction (IL-DLLME) method combined with liquid chromatography and a fluorescence detector for the analysis of ochratoxin A in rice wines is presented. The following parameters were systematically investigated: type and volume of ionic liquid, volume of dispersive solvent, salt addition, sample pH, and vortex time. Rice wine samples were first diluted to 18% alcohol with deionized water, and the pH was adjusted to 3.0. A DLLME procedure was followed that included IL ([HMIM][PF6]) and ethanol as the extraction and dispersive solvents, respectively. Under the optimized experimental conditions, good linearity was obtained with a correlation coefficient (r) of 0.9998 and a limit of detection (LOD) of 0.04μgL(-1). The recoveries ranged from 75.9% to 82.1% with an RSD below 10.4%. The proposed method was successfully applied to analyse OTA samples from several rice wine brands collected in Guangdong province, China.
Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, PR China.
Xianwen
X
Lai
Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, PR China.
Chunqiang
C
Ruan
Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, PR China.
Ruicen
R
Liu
Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: liuchenglan@scau.edu.cn.
Chenglan
C
Liu
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry; D059627:Liquid Phase Microextraction / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D012275:Oryza; D014920:Wine / Q000032:analysis*
D052578:Ionic Liquids; D009793:Ochratoxins; C025589:ochratoxin A
Dispersive liquid–liquid microextraction; HPLC-FLD; Ionic liquid; Ochratoxin A; Rice wine