The use of plethysmography in determining the severity of lung pathology in a mouse model of minimally lethal influenza virus infection.
To characterize the impact on lung function, we assessed plethysmography parameters in a course of infection with mouse-adapted A/Pennsylvania/14/2010 (H3N2) influenza virus. Several parameters, represented by enhanced pause (penh) and ratio of inspiratory/expiratory time (Ti/Te), were observed that had early (1-7dpi) and robust changes regardless of virus challenge dose. Other parameters, characterized by tidal volume (TV), breathing frequency (freq) and end inspiratory pause (EIP), changed later (7-15dpi) during the course of infection and had a virus challenge dose effect. A third category of lung function parameters, such as peak inspiratory flow, had early, virus challenge-independent changes followed by later changes that were challenge dependent. These parameters changed in a similar manner after infection with a non-mouse adapted virus, although the time-course of many parameters was delayed somewhat when compared with mouse-adapted virus. Histopathological assessment of lung samples corresponded with changes in lung function parameters. This study demonstrates the utility of plethysmography in assessing disease in a mouse model of mild influenza virus infection.
Institute for Antiviral Research, Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, United States. Electronic address: justin.julander@usu.edu.
Justin G
JG
Julander
Institute for Antiviral Research, Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, United States.
Kyle
K
Kesler
Veterinary Diagnostics Lab, Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, United States.
Arnaud J
AJ
Van Wettere
Institute for Antiviral Research, Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, United States.
John D
JD
Morrey
Institute for Antiviral Research, Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, United States.
Donald F
DF
Smee
D000818:Animals; D005260:Female; D006651:Histocytochemistry; D053122:Influenza A Virus, H3N2 Subtype / Q000254:growth & development; D008168:Lung / Q000473:pathology*; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000175:diagnosis* / Q000473:pathology*; D010991:Plethysmography / Q000379:methods*; D011024:Pneumonia, Viral / Q000175:diagnosis* / Q000473:pathology*; D012129:Respiratory Function Tests / Q000379:methods*
H3N2 variant; Influenza virus; Lung function; Mice; Plethysmography