[Construction of nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49].
OBJECTIVE
To construct nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49.


METHODS
The plasmids of pcDNA3.1 (-)E6. BARF1p. CD/UPRT. UL49 was transfected into CNE-2 cells through lipofectamine, and the transfected CNE-2 cells were selected by G418 and prodrugs for getting the cells expressing fusion CD/UPRT. UL49 gene. The protein produced by the suicide gene was tested by Western-blotting in CNE-2 cells.


RESULTS
Suicide genes were expressed stably in CNE-2 cells.


CONCLUSIONS
We constructed nasopharyngeal carcinoma cell lines CNE-2 expressing stable suicide gene through lipofectamine.
Jing
J
Qing
Suping
S
Zhao
Weihong
W
Jiang
Hua
H
Zhang
D019677:Artificial Gene Fusion / Q000379:methods*; D002277:Carcinoma; D045744:Cell Line, Tumor; D041722:Genes, Transgenic, Suicide; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*