Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy.
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
Research Institute of Molecular Pathology, Vienna, Austria.|Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.|Research Platform Quantum Phenomena & Nanoscale Biological Systems (QuNaBioS), University of Vienna, Austria.
Robert
R
Prevedel
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.|MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Young-Gyu
YG
Yoon
Research Institute of Molecular Pathology, Vienna, Austria.|Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.|Research Platform Quantum Phenomena & Nanoscale Biological Systems (QuNaBioS), University of Vienna, Austria.
Maximilian
M
Hoffmann
MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.|Department of Mechanical Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Nikita
N
Pak
MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Gordon
G
Wetzstein
Research Institute of Molecular Pathology, Vienna, Austria.
Saul
S
Kato
Research Institute of Molecular Pathology, Vienna, Austria.
Tina
T
Schrödel
MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Ramesh
R
Raskar
Research Institute of Molecular Pathology, Vienna, Austria.
Manuel
M
Zimmer
MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.|Department of Biological Engineering, Massachusetts Institute of Technology(MIT), Cambridge, MA, USA.|Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.|McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Edward S
ES
Boyden
Research Institute of Molecular Pathology, Vienna, Austria.|Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.|Research Platform Quantum Phenomena & Nanoscale Biological Systems (QuNaBioS), University of Vienna, Austria.
Alipasha
A
Vaziri
D000818:Animals; D017173:Caenorhabditis elegans; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007814:Larva / Q000648:ultrastructure; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D009474:Neurons / Q000502:physiology*; D015027:Zebrafish
D002118:Calcium