Quantification of superoxide and hydrogen peroxide in leaves.
Reactive oxygen species (ROS) are produced in plants under both non-stressful and stressful conditions. Various histochemical staining methods have been developed and are widely used to visualize ROS accumulation sites. In contrast to qualitative analysis, quantification of ROS has been time- and labor consuming. As a consequence, the number of samples, which could be analyzed in parallel, has been limited. To overcome this problem, we introduce an improved semiquantitative method, in which ROS levels are quantified after histochemical staining in plant organs with the digital image analysis package ImageJ.
Plant Physiology, Dahlem Center of Plant Sciences, Freie Universität Berlin, Königin-Luise-Straße 12-16, D-14195, Berlin, Germany.
Ilona
I
Juszczak
Margarete
M
Baier
D006861:Hydrogen Peroxide / Q000032:analysis*; D009580:Nitroblue Tetrazolium / Q000737:chemistry; D010781:Photography; D018515:Plant Leaves / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods*; D013481:Superoxides / Q000032:analysis*; D013997:Time Factors
D013481:Superoxides; D009580:Nitroblue Tetrazolium; D006861:Hydrogen Peroxide