Calcitonin gene-related peptide inhibits osteolytic factors induced by osteoblast in co-culture system with breast cancer.
Recently, it was found that α-Calcitonin gene-related peptide (CGRP) was associated with breast cancer metastases, but the role of CGRP in interaction between breast cancer and osteoblast during bone metastases is not clear. Here, we investigated the effect of CGRP on osteoblast in co-culture system with breast cancer. Using a breast cancer-osteoblast co-culture system, we chose MDA-MB-231 for breast cancer and human cell line MG-63 for osteoblast. CGRP was added to this co-culture system. The expression levels of the Runx2, RANK1, and osteoprotegerin (OPG) were analyzed using real-time PCR and western blot. CGRP receptors were investigated by immunofluorescence. We found that breast cancer cells cause osteolysis lesions by upregulating Runx2 expression, decreasing OPG expression, and increasing RANKL expression in osteoblasts. Our data prove that CGRP can regulate osteoclast coupling genes in osteoblast by increasing OPG, and decreasing RANKL and Runx2 expressions in a time-dependent manner; and inhibit those osteolytic factors induced by interaction between breast cancer cells and osteoblast. This inhibition could be abolished by the CGRP antagonist, CGRP8-37. In conclusion, calcitonin receptor-like receptor is the key player for CGRP's effect in this co-culture system.
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
Hui
H
Zhao
Lin-lin
LL
Ning
Zhi-yu
ZY
Wang
Hong-tao
HT
Li
Dan
D
Qiao
Yang
Y
Yao
Huan-long
HL
Qin
D001859:Bone Neoplasms / Q000556:secondary; D001943:Breast Neoplasms / Q000473:pathology*; D015740:Calcitonin Gene-Related Peptide / Q000037:antagonists & inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D005786:Gene Expression Regulation; D006801:Humans; D010006:Osteoblasts / Q000473:pathology*; D010014:Osteolysis / Q000188:drug therapy* / Q000473:pathology*; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism
D053244:Osteoprotegerin; D010446:Peptide Fragments; D053245:RANK Ligand; C071243:calcitonin gene-related peptide (28-37); D015740:Calcitonin Gene-Related Peptide