Determination of ochratoxin A in Italian red wines by molecularly imprinted solid phase extraction and HPLC analysis.
An extraction method based on molecularly imprinted polymer prepared through a mimic template approach was used for the determination of ochratoxin A in 17 red wines from different geographical regions of Italy. Sample loading (wine sample diluted 1:1 with 1% v/v aqueous solution of PEG 8000), washing (2 mL water/acetonitrile 4:1 v/v), and elution (2 mL of acetonitrile/acetic acid 98:2 v/v) conditions allowed the optimization of the extraction method, capable of preconcentrating ochratoxin A below the maximum permitted level of 2 ng/mL. Under optimized conditions, recoveries of ochratoxin A from spiked samples ranged from 88 to 102% with sample volumes up to 20 mL. The HPLC determination by fluorescence detection allowed limits of detection and quantification, respectively, of 0.075 and 0.225 ng/mL. Sample extractions by an immunoaffinity protocol showed the method to be comparable, demonstrating the potential of the imprinting approach to substitute for the current immunoaffinity method.
Department of Chemistry, University of Torino , Via P. Giuria 5, 10125 Torino, Italy.
Cristina
C
Giovannoli
Cinzia
C
Passini
Fabio
F
Di Nardo
Laura
L
Anfossi
Claudio
C
Baggiani
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D007558:Italy; D054802:Molecular Imprinting; D009183:Mycotoxins / Q000032:analysis* / Q000302:isolation & purification*; D009793:Ochratoxins / Q000032:analysis* / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D014920:Wine / Q000032:analysis*
D009183:Mycotoxins; D009793:Ochratoxins; D011108:Polymers; C025589:ochratoxin A