Acute effects of monounsaturated fat on postprandial lipemia and gene expression in first-degree relatives of subjects with type 2 diabetes.
OBJECTIVE
Subjects with type 2 diabetes (T2D) and their nondiabetic first-degree relatives (REL) have increased risk of cardiovascular disease (CVD). Postprandial triglyceridemia (PPL), influenced by diet, is an independent risk factor for CVD. Dietary fat elicits increased PPL in T2D compared with nondiabetic controls, but our knowledge of PPL responses to fat in REL is sparse. Our aim was to test the hypothesis that REL respond to a monounsaturated fatty acid (MUFA) challenge with a higher PPL response compared with controls who have no family history of T2D (CON) and that MUFAs exert a differential impact on incretin responses and on the expression of genes involved in carbohydrate and lipid metabolism in muscle and adipose tissues of REL and CON.


METHODS
A total of 17 REL and 17 CON consumed a meal with 72 energy percent derived from MUFAs (macadamia nut oil). Plasma triglycerides, free fatty acids, insulin, glucose, glucagon-like peptide 1, glucose-dependent insulintropic peptide and ghrelin were measured at baseline and regular intervals until 4 h postprandially. Muscle and adipose tissue biopsies were collected at baseline and at 210 min after the meal.


RESULTS
The MUFA-rich meal did not elicit different responses (P>0.05) in PPL, insulin, glucose, incretins or ghrelin in REL and CON. Several genes were differentially regulated in muscle and adipose tissues of REL and CON.


CONCLUSIONS
A MUFA-rich meal elicits similar PPL, insulin and incretin responses in REL and CON. MUFAs have a differential impact on gene expression in muscle and adipose tissues in a pattern pointing toward early defects in lipid metabolism in REL.
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark.
A
A
Pietraszek
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark.
S
S
Gregersen
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark.
S B
SB
Pedersen
The NovoNordisk Foundation Center for Basic Metabolic Research, Department of Biomedical Sciences, The Panum Institute, University of Copenhagen, Copenhagen, Denmark.
J J
JJ
Holst
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark.
K
K
Hermansen
D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000328:Adult; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism; D005190:Family; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D006949:Hyperlipidemias / Q000209:etiology*; D054795:Incretins / Q000097:blood; D007328:Insulin / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics; D039106:Macadamia / Q000737:chemistry; D008297:Male; D062407:Meals; D008875:Middle Aged; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D019518:Postprandial Period; D014280:Triglycerides / Q000097:blood
D005229:Fatty Acids, Monounsaturated; D054795:Incretins; D007328:Insulin; D014280:Triglycerides