Mussel-inspired cell-adhesion peptide modification for enhanced endothelialization of decellularized blood vessels.
Enhanced endothelialization of tissue-engineered blood vessels is essential for vascular regeneration and function of engineered vessels. In this study, mussel-inspired surface chemistry of polydopamine (pDA) coatings are applied to functionalize decellularized vein matrix (DVM) with extracellular matrix-derived cell adhesion peptides (RGD and YIGSR). DVMs engineered with pDA-peptides enhance focal adhesion, metabolic activity, and endothelial differentiation of human endothelial progenitor cells (EPCs) derived from cord blood and embryonic stem cells compared with EPCs on non-coated or pDA-coated DVMs. These results indicate that pDA-peptide functionalization may contribute to enhanced, rapid endothelialization of DVM surfaces by promoting adhesion, proliferation, and differentiation of circulating EPCs. Ultimately, this approach may be useful for improving in vivo patency and function of decellularized matrix-based blood vessels.
Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749, Republic of Korea.
Jung Seung
JS
Lee
Kihong
K
Lee
Sung-Hwan
SH
Moon
Hyung-Min
HM
Chung
Jun Hyup
JH
Lee
Soong Ho
SH
Um
Dong-Ik
DI
Kim
Seung-Woo
SW
Cho
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D001808:Blood Vessels / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D066026:Endothelial Progenitor Cells / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D022001:Focal Adhesions / Q000502:physiology; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D014654:Vascular Patency / Q000502:physiology
D016326:Extracellular Matrix Proteins; D007211:Indoles; D009842:Oligopeptides; D011108:Polymers; C568283:polydopamine; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; C047981:arginyl-glycyl-aspartic acid
cell adhesion peptide; decellularized vein matrix; endothelial progenitor cells; endothelialization; polydopamine