A novel cell disruption technique to enhance lipid extraction from microalgae.
Lipid extraction represents one of the main bottlenecks of the microalgal technology for the production of biofuels. A novel method based on the use of H2O2 with or without FeSO4, to disrupt the cell wall of Chlorella vulgaris and favor the subsequent extraction of lipids from wet biomass, is proposed. Experimental results show that, when disruption is performed under suitable operating conditions, the amount of lipids extracted is significantly increased with respect to the case where a classical approach is applied. Moreover, quality of lipids extracted after disruption seems to be improved in view of their exploitation for producing biofuels.
Research Unit of the National Interuniversity Consortium "The Chemistry for the Environment" and Interdepartmental Center of Environmental Science and Engineering (CINSA), University of Cagliari, Via San Giorgio 12, 09124 Cagliari, Italy.
Alberto
A
Steriti
Research Unit of the National Interuniversity Consortium "The Chemistry for the Environment" and Interdepartmental Center of Environmental Science and Engineering (CINSA), University of Cagliari, Via San Giorgio 12, 09124 Cagliari, Italy.
Roberto
R
Rossi
Center for Advanced Studies, Research and Development in Sardinia (CRS4), Loc. Piscina Manna, Building 1, 09010 Pula (CA), Italy. Electronic address: aconcas@crs4.it.
Alessandro
A
Concas
Research Unit of the National Interuniversity Consortium "The Chemistry for the Environment" and Interdepartmental Center of Environmental Science and Engineering (CINSA), University of Cagliari, Via San Giorgio 12, 09124 Cagliari, Italy; Center for Advanced Studies, Research and Development in Sardinia (CRS4), Loc. Piscina Manna, Building 1, 09010 Pula (CA), Italy; Department of Mechanical, Chemical and Materials Engineering, University of Cagliari, Piazza d'Armi, 09123 Cagliari, Italy. Electronic address: giacomo.cao@dimcm.unica.it.
Giacomo
G
Cao
D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002138:Calibration; D048408:Chlorella vulgaris / Q000254:growth & development / Q000378:metabolism*; D004952:Esters / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D008055:Lipids / Q000302:isolation & purification*; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D058433:Photobioreactors
D004952:Esters; D005227:Fatty Acids; D008055:Lipids
Cell disruption; Fenton’s reaction; Lipid extraction; Microalgae