Archived formalin-fixed paraffin-embedded (FFPE) blocks: A valuable underexploited resource for extraction of DNA, RNA, and protein.
Formalin-fixed paraffin-embedded (FFPE) material presents a readily available resource in the study of various biomarkers. There has been interest in whether the storage period has significant effect on the extracted macromolecules. Thus, in this study, we investigated if the storage period had an effect on the quantity/quality of the extracted nucleic acids and proteins. We systematically examined the quality/quantity of genomic DNA, total RNA, and total protein in the FFPE blocks of malignant tumors of lung, thyroid, and salivary gland that had been stored over several years. We show that there is no significant difference between macromolecules extracted from blocks stored over 11-12 years, 5-7 years, or 1-2 years in comparison to the current year blocks.
Cooperative Human Tissue Network-Eastern Division, Department of Pathology and Lab Medicine, Perelman School of Medicine, University of Pennsylvania , Philadelphia, Pennsylvania.
Theresa J
TJ
Kokkat
Miral S
MS
Patel
Diane
D
McGarvey
Virginia A
VA
LiVolsi
Zubair W
ZW
Baloch
D004247:DNA / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005557:Formaldehyde / Q000737:chemistry*; D005615:Freezing; D006801:Humans; D016612:Paraffin Embedding / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*; D012313:RNA / Q000302:isolation & purification*; D016707:Tissue Fixation / Q000379:methods*
D011506:Proteins; D005557:Formaldehyde; D012313:RNA; D004247:DNA