Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy.
Cytotoxic T cells typically are expanded ex vivo in culture with IL2 for adoptive immunotherapy. This culture period leads to a differentiated phenotype and acquisition of effector function, as well as a loss of in vivo proliferative capability and antitumor efficacy. Here, we report antigen-specific and polyclonal expansion of cytotoxic T cells in a cocktail of cytokines and small molecules that leads to a memory-like phenotype in mouse and human cells even during extended culture, leading to enhanced in vivo expansion and tumor control in mice.
Masonic Cancer Center, Brain Tumor Program, Departments of Pediatrics.
Adam J
AJ
Litterman
Masonic Cancer Center, Brain Tumor Program, Departments of Pediatrics.
David M
DM
Zellmer
Masonic Cancer Center, Genetics, Cell Biology and Development, and Center for Genome Engineering, and.
Rebecca S
RS
LaRue
Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota, Minneapolis, Minnesota.
Stephen C
SC
Jameson
Masonic Cancer Center, Brain Tumor Program, Departments of Pediatrics, Genetics, Cell Biology and Development, and Center for Genome Engineering, and larga002@umn.edu.
David A
DA
Largaespada
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006801:Humans; D007156:Immunologic Memory; D016219:Immunotherapy, Adoptive; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000628:therapy
D016207:Cytokines; D000939:Epitopes