Determination of parasitic load in different tissues of murine toxoplasmosis after immunization by excretory-secretory antigens using Real time QPCR.
Excretory-secretory antigens (ESAs) of Toxoplasma gondii are one of the candidates for immunization against toxoplasmosis. For evaluation of immunization, we determined the kinetics of the distribution of Toxoplasma and parasite load in different tissues of mice immunized by ESAs. In this experimental study, 36 mice in case (n=18) and control (n=18) groups were immunized with ESAs and PBS, respectively. After 2weeks, mice were challenged intraperitoneally with Toxoplasma virulent RH strain. Blood and different tissues (brain, spleen, liver, heart, kidney, and muscle) were collected daily after challenge (1, 2, 3 and last day before death). Parasite load was calculated using Real time QPCR targeted at the B1 gene. ESAs as vaccine in different tissues showed various effects. However, infected mice which received the vaccine in comparison with control group, displayed a drastically decreasing in parasite burden, in their blood and tissues (P=0.000). These results indicated that ESAs with reduction of parasite load in different tissues of host could be evaluable candidate for the development of immunization strategies against toxoplasmosis.
Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Parasitology and Mycology Department, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran.
Ahmad
A
Daryani
Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Parasitology and Mycology Department, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran. Electronic address: msharifmahdi@yahoo.com.
Mehdi
M
Sharif
Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Parasitology and Mycology Department, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran.
Yousef
Y
Dadimoghaddam
Department of Biochemistry, Biophysics & Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Mohammad Bagher Hashemi
MB
Souteh
Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Parasitology and Mycology Department, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran.
Ehsan
E
Ahmadpour
Biostatistics Department, Mazandaran University of Medical Sciences, Sari, Iran.
Alireza
A
Khalilian
Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Parasitology and Mycology Department, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran.
Shahabeddin
S
Sarvi
Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Touraj
T
Farazmand
Parasitology and Mycology Department, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran.
Hamed
H
Kalani
Department of Biochemistry, Biophysics & Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Mehdi
M
Rasouli
D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D001921:Brain / Q000469:parasitology; D016054:DNA, Protozoan / Q000302:isolation & purification; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000469:parasitology; D059208:Parasite Load; D018512:Parasitemia / Q000469:parasitology; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D014122:Toxoplasma / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000469:parasitology / Q000517:prevention & control*; D014781:Viscera / Q000469:parasitology
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D005620:Freund's Adjuvant
Excretory–secretory antigens; Parasite load; QPCR; Toxoplasmosis; Vaccine