Polyetheretherketone/nano-fluorohydroxyapatite composite with antimicrobial activity and osseointegration properties.
Lack of antibacterial activity and binding ability to natural bone tissue has significantly limited polyetheretherketone (PEEK) for many challenging dental implant applications. Here, we have developed a polyetheretherketone/nano-fluorohydroxyapatite (PEEK/nano-FHA) biocomposite with enhanced antibacterial activity and osseointegration through blending method. Smooth and rough surfaces of PEEK/nano-FHA biocomposites were also prepared. Our results showed that in vitro initial cell adhesion and proliferation on the nano-FHA reinforced PEEK composite were improved. In addition, higher alkaline phosphatase activity and cell mineralization were also detected in cells cultured on PEEK/nano-FHA biocomposites, especially for rough PEEK/nano-FHA surfaces. More importantly, the as-prepared PEEK/nano-FHA biocomposite could effectively prevent the proliferation and biofilm formation of bacterial. For in vivo test, the newly formed bone volume of PEEK/nano-FHA group was higher than that of bare PEEK group based on 3D microcomputed tomography and 2D histomorphometric analysis. These reports demonstrate that the developed PEEK/nano-FHA biocomposite has increased biocompatibility and antibacterial activity in vitro, and promoted osseointegration in vivo, which suggests that it holds potential to be applied as dental implant material in dental tissue engineering applications.
Department of Stomatology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China.
Lixin
L
Wang
Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
Shu
S
He
Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing 401147, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
Xiaomian
X
Wu
The Affiliated Hospital, Hainan Medical College, Hainan 571199, China.
Shanshan
S
Liang
The Affiliated Hospital, Hainan Medical College, Hainan 571199, China.
Zhonglin
Z
Mu
Key Laboratory for Ultrafine Materials of Ministry of Education, Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China.
Jie
J
Wei
Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing 401147, China.
Feng
F
Deng
Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: dengyibandeng@pku.edu.cn.
Yi
Y
Deng
Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing 100081, China; Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing 401147, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: sc-wei@pku.edu.cn.
Shicheng
S
Wei
D000469:Alkaline Phosphatase / Q000378:metabolism; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D001577:Benzophenones; D001672:Biocompatible Materials / Q000737:chemistry; D018441:Biofilms / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry* / Q000494:pharmacology; D007659:Ketones / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry*; D016348:Osseointegration / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers; D013295:Streptococcus mutans / Q000187:drug effects; D055114:X-Ray Microtomography
D000890:Anti-Infective Agents; D001577:Benzophenones; D001672:Biocompatible Materials; D006882:Hydroxyapatites; D007659:Ketones; D011108:Polymers; C116586:fluorohydroxyapatite; C063834:polyetheretherketone; D011092:Polyethylene Glycols; D000469:Alkaline Phosphatase
Antimicrobial; Bioactivity; Fluorohydroxyapatite; Implant; Osseointegration; Polyetheretherketone