(1)H NMR metabolomics reveals contrasting response by male and female mussels exposed to reduced seawater pH, increased temperature, and a pathogen.
Human activities are fundamentally altering the chemistry of the world's oceans. Ocean acidification (OA) is occurring against a background of warming and an increasing occurrence of disease outbreaks, posing a significant threat to marine organisms, communities, and ecosystems. In the current study, (1)H NMR spectroscopy was used to investigate the response of the blue mussel, Mytilus edulis, to a 90-day exposure to reduced seawater pH and increased temperature, followed by a subsequent pathogenic challenge. Analysis of the metabolome revealed significant differences between male and female organisms. Furthermore, males and females are shown to respond differently to environmental stress. While males were significantly affected by reduced seawater pH, increased temperature, and a bacterial challenge, it was only a reduction in seawater pH that impacted females. Despite impacting males and females differently, stressors seem to act via a generalized stress response impacting both energy metabolism and osmotic balance in both sexes. This study therefore has important implications for the interpretation of metabolomic data in mussels, as well as the impact of environmental stress in marine invertebrates in general.
Plymouth Marine Laboratory , Prospect Place, West Hoe, Plymouth, PL1 3DH, U.K.
Robert P
RP
Ellis
John I
JI
Spicer
Jonathan J
JJ
Byrne
Ulf
U
Sommer
Mark R
MR
Viant
Daniel A
DA
White
Steve
S
Widdicombe
D000825:Animal Structures / Q000378:metabolism; D000818:Animals; D002254:Carbonates / Q000737:chemistry; D004734:Energy Metabolism; D004781:Environmental Exposure / Q000032:analysis*; D005260:Female; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D049880:Mytilus edulis / Q000378:metabolism* / Q000382:microbiology*; D012623:Seawater / Q000737:chemistry*; D013312:Stress, Physiological; D013696:Temperature; D014733:Vibrio / Q000502:physiology
D002254:Carbonates