Crystal structure of the secreted protein HP1454 from the human pathogen Helicobacter pylori.
HP1454 is a protein of 303 amino acids found in the extracellular milieu of Helicobacter pylori. The protein structure, crystallized in the orthorhombic C222₁ space group with one molecule per asymmetric unit, has been determined using the single-wavelength anomalous dispersion method. HP1454 exhibits an elongated bent shape, composed of three distinct domains. Each domain possesses a fold already present in other structures: Domain I contains a three-strand antiparallel β-barrel flanked by a long α-helix, Domain II is an anti-parallel three-helix bundle, and Domain III a β-sheet flanked by two α-helices. The overall assembly of the protein does not bear any similarity with known structures.
Department of Biomedical Sciences, University of Padua, Viale G. Colombo 3, Padua, 35131, Italy.
Sandra
S
Quarantini
Laura
L
Cendron
Giuseppe
G
Zanotti
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D030562:Databases, Protein; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008074:Lipoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins; C089630:Lpp20 protein, Helicobacter pylori; D011994:Recombinant Proteins
Helicobacter pylori; outer membrane; secreted proteins