A refined technique for sciatic denervation in a golden-mantled ground squirrel (Callospermophilus lateralis) model of disuse atrophy.
Disuse atrophy of both muscle and bone can occur rapidly during periods of inactivity. In several rodent models developed for the study of disuse atrophy, immobilization is induced by prolonged cage restraint, hind limb unloading, tenotomy, sciatic nerve block or sciatic denervation. In less tractable species such as wild-caught hibernating rodents, the sciatic denervation model is superior in terms of both animal welfare and applicability to the characteristics of natural cases of disuse atrophy. The authors describe a refined surgical approach to sciatic denervation in golden-mantled ground squirrels (Callospermophilus lateralis), a hibernating species, that improves animal welfare and reduces the incidence of post-operative complications such as autotomy.
Laboratory Animal Medicine, University of Nevada, Las Vegas, NV.
Valeri
V
Sarukhanov
Laboratory Animal Medicine, University of Nevada, Las Vegas, NV.
Roger
R
Van Andel
School of Life Sciences, University of Nevada, Las Vegas, NV.
Michael D
MD
Treat
School of Life Sciences, University of Nevada, Las Vegas, NV.
Jenifer C
JC
Utz
School of Life Sciences, University of Nevada, Las Vegas, NV.
Frank
F
van Breukelen
D000827:Animal Welfare / Q000592:standards; D000818:Animals; D003714:Denervation / Q000379:methods* / Q000662:veterinary*; D004195:Disease Models, Animal; D020966:Muscular Disorders, Atrophic; D012584:Sciatic Nerve / Q000601:surgery*; D012589:Sciuridae / Q000601:surgery*