Effect of variation of ABCB1 and GSTP1 on osteosarcoma survival after chemotherapy.
We conducted a comprehensive study to investigate the role of genes involved in metabolic and transport pathways in response to chemotherapy and clinical outcome of osteosarcoma patients. Genotyping of seven gene polymorphisms was performed on a 384-well plate format on the Sequenom MassARRAY platform in 162 patients with osteosarcoma. We studied the correlation of the seven gene polymorphisms with response to chemotherapy and clinical outcome of patients. Individuals with the ABCB1 TT genotype had a higher probability of responding poorly to chemotherapy, indicated by an odds ratio (OR) of 2.64 (95%CI=1.04-6.83). Similarly, the genotype of GSTP1 GG was significantly associated with improved responses to chemotherapy, indicated by an OR of 3.33 (95%CI=1.26-8.99). The ABCB1 TT and GSTP1 GG genotypes were significantly associated with a shorter overall survival (OS). Our study found that two gene polymorphisms in two transporter genes and one Phase II metabolism enzymes are associated with response to chemotherapy and OS in osteosarcoma patients, suggesting the potential of the two gene polymorphisms as prognostic biomarkers for osteosarcoma.
Department of Orthopedics, Juye County People's Hospital, Heze, China.
J Z
JZ
Li
Department of Orthopedics, The Third People's Hospital of Heze, Heze, China.
Z Q
ZQ
Tian
Department of Spinal Surgery, Infectious Disease Hospital of Heilongjiang, Harbin, China.
S N
SN
Jiang
Department of Orthopedics, Heze Municipal Hospital, Heze, China xuyuanlan699@163.com.
T
T
Feng
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D000293:Adolescent; D000328:Adult; D054316:Biomarkers, Pharmacological; D001859:Bone Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D002648:Child; D018572:Disease-Free Survival; D004317:Doxorubicin / Q000008:administration & dosage; D005260:Female; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D008297:Male; D050217:Metabolic Detoxication, Phase II / Q000235:genetics; D008875:Middle Aged; D012516:Osteosarcoma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis
C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D054316:Biomarkers, Pharmacological; D004317:Doxorubicin; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi