[Determination of homocysteine by tandem mass spectrometry with chemical ionization].
Homocysteine (Hcy) is an intermediate of methionine metabolism. High plasma Hcy concentrations are an independent risk factor for stroke, peripheral vascular disease, deep venous thrombosis, coronary disease, and cognitive deficiency. Apparently, it is a great importance to measure Hcy levels in human blood. A new method for the quantification of Hcy by means of reversed-phase LC/atmospheric pressure chemical ionization mass spectrometry has been developed. The MRM ion transition, m/z 136.0 ® 90.0 was used for Hcy quantification. The limit of detection was 0.4 mM, quantification was performed from 1 mM to 40 mM with coefficient of determination of R2=0,997. The method was applied successfully to Hcy determination in human blood.
I I
II
Miroshnichenko
A I
AI
Platova
T P
TP
Safarova
O B
OB
Iakovleva
D000368:Aged; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003863:Depression / Q000097:blood; D005260:Female; D006710:Homocysteine / Q000097:blood* / Q000737:chemistry; D006801:Humans; D020138:Hyperhomocysteinemia / Q000097:blood; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D013048:Specimen Handling; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
D006710:Homocysteine
APCI; MRM; homocysteine; liquid chromatography tandem mass spectrometry (LC-MS/MS)