Increased expressions of NKp44, NKp46 on NK/NKT-like cells are associated with impaired cytolytic function in self-limiting hepatitis E infection.
We have characterized the NK/NKT-like cells in patients with self-limiting hepatitis E infection. The distribution of peripheral NK/NKT-like cells, expressions of activation receptors, cytotoxic potential and effector function of NK/NKT-like cells from fresh peripheral blood mononuclear cells of 86 acute patients, 101 recovered and 54 control individuals were assessed. Activated NKT-like (CD16(+) CD56(+) CD3(+)) cells were high in the patient groups. On CD56(+) CD3(-) cells, NKp44 and NKp46 expressions were high in the acute patients, whereas NKp30, NKp44, NKp46 and NKG2D were high in the recovered individuals. On CD56(+) CD3(+) cells, NKp44, NKp46 and NKG2D expressions were high in the recovered but NKp30 was low in both the patient groups. Collectively, the current study elucidates the role of NK/NKT-like cells demonstrating phenotypic alterations of activated NKT-like cells and activation receptors, lack of CD107a expression and functional impairment of peripheral NK/NKT-like cells in self-limiting hepatitis E infection.
Hepatitis Group, MCC, National Institute of Virology, 130/1, Sus Road, Pashan, Pune, 411021, Maharashtra, India.
Rumki
R
Das
Anuradha
A
Tripathy
D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D016751:Hepatitis E / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry / Q000502:physiology*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000032:analysis; D055627:Natural Cytotoxicity Triggering Receptor 1 / Q000096:biosynthesis*; D055629:Natural Cytotoxicity Triggering Receptor 2 / Q000096:biosynthesis*; D055628:Natural Cytotoxicity Triggering Receptor 3 / Q000032:analysis; D055611:Natural Killer T-Cells / Q000737:chemistry / Q000502:physiology*; D055815:Young Adult
D015703:Antigens, CD; C527085:KLRK1 protein, human; C112260:NCR1 protein, human; C112837:NCR2 protein, human; C431710:NCR3 protein, human; D055655:NK Cell Lectin-Like Receptor Subfamily K; D055627:Natural Cytotoxicity Triggering Receptor 1; D055629:Natural Cytotoxicity Triggering Receptor 2; D055628:Natural Cytotoxicity Triggering Receptor 3