Identification of 10 cowpox virus proteins that are necessary for induction of hemorrhagic lesions (red pocks) on chorioallantoic membranes.
Cowpox viruses (CPXV) cause hemorrhagic lesions ("red pocks") on infected chorioallantoic membranes (CAM) of embryonated chicken eggs, while most other members of the genus Orthopoxvirus produce nonhemorrhagic lesions ("white pocks"). Cytokine response modifier A (CrmA) of CPXV strain Brighton Red (BR) is necessary but not sufficient for the induction of red pocks. To identify additional viral proteins involved in the induction of hemorrhagic lesions, a library of single-gene CPXV knockout mutants was screened. We identified 10 proteins that are required for the formation of hemorrhagic lesions, which are encoded by CPXV060, CPXV064, CPXV068, CPXV069, CPXV074, CPXV136, CPXV168, CPXV169, CPXV172, and CPXV199. The genes are the homologues of F12L, F15L, E2L, E3L, E8R, A4L, A33R, A34R, A36R, and B5R of vaccinia virus (VACV). Mutants with deletions in CPXV060, CPXV168, CPXV169, CPXV172, or CPXV199 induced white pocks with a comet-like shape on the CAM. The homologues of these five genes in VACV encode proteins that are involved in the production of extracellular enveloped viruses (EEV) and the repulsion of superinfecting virions by actin tails. The homologue of CPXV068 in VACV is also involved in EEV production but is not related to actin tail induction. The other genes encode immunomodulatory proteins (CPXV069 and crmA) and viral core proteins (CPXV074 and CPXV136), and the function of the product of CPXV064 is unknown.


OBJECTIVE
It has been known for a long time that cowpox virus induces hemorrhagic lesions on chicken CAM, while most of the other orthopoxviruses produce nonhemorrhagic lesions. Although cowpox virus CrmA has been proved to be responsible for the hemorrhagic phenotype, other proteins causing this phenotype remain unknown. Recently, we generated a complete single-gene knockout bacterial artificial chromosome (BAC) library of cowpox virus Brighton strain. Out of 183 knockout BAC clones, 109 knockout viruses were reconstituted. The knockout library makes possible high-throughput screening for studying poxvirus replication and pathogenesis. In this study, we screened all 109 single-gene knockout viruses and identified 10 proteins necessary for inducing hemorrhagic lesions. The identification of these genes gives a new perspective for studying the hemorrhagic phenotype and may give a better understanding of poxvirus virulence.
Institut für Virologie, Freie Universität Berlin, Zentrum für Infektionsmedizin, Berlin, Germany.
Zhiyong
Z
Xu
Institut für Virologie, Freie Universität Berlin, Zentrum für Infektionsmedizin, Berlin, Germany.
Dimitrios
D
Zikos
Institut für Virologie, Freie Universität Berlin, Zentrum für Infektionsmedizin, Berlin, Germany.
Aistė
A
Tamošiūnaitė
Institut für Tierpathologie, Freie Universität Berlin, Berlin, Germany.
Robert
R
Klopfleisch
Institut für Virologie, Freie Universität Berlin, Zentrum für Infektionsmedizin, Berlin, Germany no.34@fu-berlin.de k.tischer@fu-berlin.de.
Nikolaus
N
Osterrieder
Institut für Virologie, Freie Universität Berlin, Zentrum für Infektionsmedizin, Berlin, Germany no.34@fu-berlin.de k.tischer@fu-berlin.de.
B Karsten
BK
Tischer
D000818:Animals; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000473:pathology* / Q000821:virology*; D015608:Cowpox virus / Q000235:genetics / Q000502:physiology*; D055786:Gene Knockout Techniques; D006470:Hemorrhage / Q000473:pathology / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
D014764:Viral Proteins; D037521:Virulence Factors