Nanofibers coated on acellular tissue-engineered bovine pericardium supports differentiation of mesenchymal stem cells into endothelial cells for tissue engineering.
OBJECTIVE
This study aimed to develop biodegradable, polymer-based nanofibers coated on acellular tissue-engineered bovine pericardium (ATEBP) for cell interfaces, enabling more exquisite functionality, such as mesenchymal stem cell (MSC) adhesion, proliferation and differentiation into endothelial cells for tissue engineering.


METHODS
ATEBP coated with nanofibers of poly(L-lactic acid)-co-poly(ε-caprolactone) (PLACL) and a blend of PLACL and gelatin were analyzed for human bone marrow-derived MSC adhesion, proliferation and differentiation into endothelial cells.


RESULTS
The cell culture-based approach showed an increase in human bone marrow-derived MSC adhesion, proliferation and differentiation into endothelial cells on ATEBP coated with PLACL/gelatin nanofibers compared with ATEBP and PLACL nanofibers coated on ATEBP.


CONCLUSIONS
ATEBP coated with PLACL/gelatin nanofibrous scaffolds, along with human bone marrow-derived MSCs differentiated into endothelial cells, might improve the scaffolds' functionality for tissue engineering.
Stem Cell & Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, Tamil Nadu, India.
Santosh
S
Mathapati
Dillip Kumar
DK
Bishi
Jayarama Reddy
JR
Venugopal
Kotturathu Mammen
KM
Cherian
Soma
S
Guhathakurta
Seeram
S
Ramakrishna
Rama Shanker
RS
Verma
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D002474:Cell-Free System; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology; D019544:Equipment Failure Analysis; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010496:Pericardium / Q000737:chemistry*; D011474:Prosthesis Design; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds
D020099:Coated Materials, Biocompatible
bovine pericardium; endothelial cell; mesenchymal stem cell; nanofiber; tissue engineering