Electrochemical immunosensor for detection of topoisomerase based on graphene-gold nanocomposites.
A facile electrochemical immunosensor based on graphene-three dimensional nanostructure gold nanocomposites (G-3D Au) using simple and rapid one-step electrochemical co-reduction technique was developed for sensitive detection of topoisomerase. The resultant G-3D Au nanocomposites were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy, and then were used as a substrate for construction of the "sandwich-type" immunosensor. Amperometric current-time curve was employed to monitor the immunoreaction on the protein modified electrode. The proposed method could respond to topoisomerase with a linear calibration range from 0.5 ng mL(-1) to 50 ng mL(-1) with a detection limit of 10 pg mL(-1). This new biosensor exhibited a fast amperometric response, high sensitivity and selectivity, and was successfully used in determining the topoisomerase which was added in human serum with a relative standard deviation (n=5)<5%. The immunosensor served as a significant step toward the practical application of the immunosensor in clinical diagnosis and prognosis monitor.
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, PR China; Department of Orthopaedics, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China.
Guang-Xian
GX
Zhong
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, PR China.
Peng
P
Wang
Department of Endocrinology, The County Hospital of Anxi, Anxi 362400, PR China.
Fei-Huan
FH
Fu
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, PR China.
Shao-Huang
SH
Weng
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, PR China.
Wei
W
Chen
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, PR China.
Shao-Guang
SG
Li
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, PR China. Electronic address: ailinliu@mail.fjmu.edu.cn.
Ai-Lin
AL
Liu
Department of Orthopaedics, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China.
Zhao-Yang
ZY
Wu
Department of Orthopaedics, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China.
Xia
X
Zhu
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, PR China. Electronic address: xhl1963@sina.com.
Xin-Hua
XH
Lin
Department of Orthopaedics, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China. Electronic address: jianhual@126.com.
Jian-Hua
JH
Lin
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Xing-Hua
XH
Xia
D015374:Biosensing Techniques; D004264:DNA Topoisomerases, Type I / Q000032:analysis* / Q000097:blood; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D036103:Nanotechnology; D009369:Neoplasms / Q000175:diagnosis; D015203:Reproducibility of Results
D006046:Gold; D006108:Graphite; D004264:DNA Topoisomerases, Type I
Electrochemical immunosensor; Graphene–gold nanocomposites; Topoisomerase