(60)Co-γ irradiation affects the enzymatic antioxidant system of the citrus red mite Panonychus citri (Acari: Tetranychidae).
Radio-(60Co), which emits γ rays, has been used worldwide in pest control. The aim of the present study was to analyze the effect of effective-low-power 60Co-γ irradiation on the enzymatic antioxidant system of the citrus red mite Panonychus citri. One day old female adults were exposed to 0.4 kGy 60Co-γ irradiation and on the, 6th h, 1st day, 2nd day, and 5th day post treatment, the mites were euthanized for biochemical analysis. The activity of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), phenoloxidase (PO) and acetylocholinesterase (AchE) were investigated. POD and CAT activities, as well as SOD were higher in the irradiated mites. We found that exposure to 60Co-γ radiation resulted in increased activities of SOD, CAT, POD and decreased AchE activity. When the recovery time lasted till the 5th day, the activities of POD and PO were significantly lower than the control, whereas the SOD, CAT and AchE activities returned to control levels. Cells possess protein repair pathways to rescue oxidized proteins and restore their functions, but if these repair processes fail, oxidized proteins may become cytotoxic. Our results confirm the hypothesis that low dosages of 60Co-γ irradiation increase the level of oxidative stress in P. citri adults in a short time, causing meanwhile damage and sterility. The results of this study also indicate that stress caused by exposure to irradiation could inhibit the cholinergic system in P. citri.
Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Guangzhou 510642, China. zhangke2110@163.com.
Ke
K
Zhang
Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Guangzhou 510642, China. zhibinli00@163.com.
Zhibin
Z
Li
Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Guangzhou 510642, China. chuk_shiuman@163.com.
Shaowen
S
Zhu
Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Guangzhou 510642, China. huabao@scau.edu.cn.
Qunfang
Q
Weng
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003037:Cobalt Radioisotopes; D004307:Dose-Response Relationship, Radiation; D004798:Enzymes / Q000378:metabolism*; D005260:Female; D005720:Gamma Rays; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010063:Ovum / Q000528:radiation effects; D018384:Oxidative Stress / Q000528:radiation effects; D010544:Peroxidases / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D040102:Tetranychidae / Q000201:enzymology* / Q000528:radiation effects*
D000975:Antioxidants; D003037:Cobalt Radioisotopes; D004798:Enzymes; D010544:Peroxidases; D002374:Catalase; D014442:Monophenol Monooxygenase; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase