Membranes, peptides, and disease: unraveling the mechanisms of viral proteins with solid state nuclear magnetic resonance spectroscopy.
The interplay between peptides and lipid bilayers drives crucial biological processes. For example, a critical step in the replication cycle of enveloped viruses is the fusion of the viral membrane and host cell endosomal membrane, and these fusion events are controlled by viral fusion peptides. Thus such membrane-interacting peptides are of considerable interest as potential pharmacological targets. Deeper insight is needed into the mechanisms by which fusion peptides and other viral peptides modulate their surrounding membrane environment, and also how the particular membrane environment modulates the structure and activity of these peptides. An important step toward understanding these processes is to characterize the structure of viral peptides in environments that are as biologically relevant as possible. Solid state nuclear magnetic resonance (ssNMR) is uniquely well suited to provide atomic level information on the structure and dynamics of both membrane-associated peptides as well as the lipid bilayer itself; further ssNMR can delineate the contribution of specific membrane components, such as cholesterol, or changing cellular conditions, such as a decrease in pH on membrane-associating peptides. This paper highlights recent advances in the study of three types of membrane associated viral peptides by ssNMR to illustrate the more general power of ssNMR in addressing important biological questions involving membrane proteins.
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037 USA.
Matthew T
MT
Eddy
Institute of Atomic and Molecular Sciences, Academia Sinica, No. 1 Sec. 4. Rooservelt Rd., Taipei, 10617, Taiwan. Electronic address: dharmanmr@gate.sinica.edu.tw.
Tsyr-Yan
TY
Yu
D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins / Q000502:physiology*; D010455:Peptides / Q000502:physiology*; D014764:Viral Proteins / Q000502:physiology*; D014777:Virus Diseases / Q000821:virology*
D008565:Membrane Proteins; D010455:Peptides; D014764:Viral Proteins
Membrane associate viral protein; Membrane associated viral peptides; Protein structure and function; ssNMR