Biomolecular logic gate for analysis of the New Delhi metallo-β-lactamase (NDM)-coding gene with concurrent determination of its drug resistance-encoding fragments.
We herein exploit a newly schemed logic gate to superiorly facilitate analysis of long and highly structured nucleic acids. This strategy uniquely enables the identification of NDM-specific genes and concurrent screening of two active site-encoded fragments, which is promising for evaluating microbial drug resistance.
Department of Chemistry, Tunghai University, Taichung, Taiwan. mcchuang@thu.edu.tw.
Yu-Hsuan
YH
Lai
Yu-Chieh
YC
Liao
Jung-Jung
JJ
Mu
Tsui-Ming
TM
Kuo
Yuan-Hao
YH
Hsu
Min-Chieh
MC
Chuang
D000465:Algorithms; D000595:Amino Acid Sequence; D001483:Base Sequence; D020134:Catalytic Domain / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D007118:Immunoassay; D007711:Klebsiella pneumoniae / Q000201:enzymology; D009696:Nucleic Acids / Q000032:analysis*; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics
D004800:Enzymes, Immobilized; D009696:Nucleic Acids; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; D016650:Fluorescein-5-isothiocyanate