[High frequency of DQ8 in the celiac population of Chaco province, Argentina].
BACKGROUND
There is a strong association between celiac disease (CD) and certain genes of the major histocompatibility complex (HLA). The CD specifically related alleles are those coding for HLA-DQ2 heterodimer and to a lesser degree for HLA-DQ8. OBJECTVE. The aim of this study was to evaluate the frequency of HLA-DQB1* and HLA-DRB1* alleles, haplotypes, and genotypes in patients diagnosed with CD and in control population of Chaco, in order to establish its distribution and compare it with that observed in other populations.


METHODS
A total of 139 samples from patients diagnosed with CD and 119 healthy controls were typed for HLA-DQ and HLA-DR, using PCR and reverse hybridization (INNO-LiPA or Dynal).


RESULTS
Comparing patients with CD vs. controls, the DQBI*0201 (P = 0.0002), DQBJ*0202 (P = 0.0046), DQBI*0302 (P = 0. 0006), DRBl *03 (P = 0.0002), DRBl *04 (P = 0.0199) and DRB1 *07 (P = 0.0062) were significantly increased, while a decrease was observed in HLA-DQB1*0301 (P = 0.0006), HLA-DQBI*0303 (P = 0.0070), DQBI*0501 (P = 0.0023), DQB1*0604 (P = 0.0140) DRB1*01 (P = 0.0023), DRB1*08 (P = 0.0165), DRB1*09 (P = 0.0362) and DRB1*16 (P = 0.0228). Within DQB1* genotypes associated with EC, 65.4% of patients had the DQB1*02 in linkage disequilibrium with DRB1*03 or DRB1*07 (DQ2), and 43.2% presented genotype DQB1*0302 in linkage disequilibrium with DRB1*04 (DQ8). Both genotypes were shared by 15.2% of them.


CONCLUSIONS
We point out the high frequency of DQ8 associated with CD. Although the DQ2 is still the most common, this finding could be attributed to the Amerindian influence in our population.
Patricía Maria F
PM
Motta
María A
MA
López
Karina
K
Marinic
Santiago O
SO
Picón
Mariana G
MG
Stafuza
Alicia
A
Habegger de Sorrentino
D000293:Adolescent; D000328:Adult; D000368:Aged; D001118:Argentina; D016022:Case-Control Studies; D002446:Celiac Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D006683:HLA-DQ Antigens; C109756:HLA-DQ8 antigen; D059811:HLA-DRB1 Chains