Separation of nine compounds from Salvia plebeia R.Br. using two-step high-speed counter-current chromatography with different elution modes.
Nine compounds were successfully separated from Salvia plebeia R.Br. using two-step high-speed counter-current chromatography with three elution modes. Elution-extrusion counter-current chromatography was applied in the first step, while classical counter-current chromatography and recycling counter-current chromatography were used in the second step. Three solvent systems, n-hexane/ethyl acetate/ethanol/water (4:6.5:3:7, v/v), methyl tert-butyl ether/ethyl acetate/n-butanol/methanol/water (6:4:1:2:8, v/v) and n-hexane/ethyl acetate/methanol/water (5:5.5:5:5, v/v) were screened and optimized for the two-step separation. The separation yielded nine compounds, including caffeic acid (1), 6-hydroxyluteuolin-7-glucoside (2), 5,7,3',4'-tetrahydroxy-6-methoxyflavanone-7-glucoside (3), nepitrin (4), rosmarinic acid (5), homoplantaginin (6), nepetin (7), hispidulin (8), and 5,6,7,4'-tertrahydroxyflavone (9). To the best of our knowledge, 5,7,3',4'-tetrahydroxy-6-methoxyflavanone-7-glucoside and 5,6,7,4'-tertrahydroxyflavone have been separated from Salvia plebeia R.Br. for the first time. The purities and structures of these compounds were identified by high-performance liquid chromatography, electrospray ionization mass spectrometry, (1)H and (13)C NMR spectroscopy. This study demonstrates that high-speed counter-current chromatography is a useful and flexible tool for the separation of components from a complex sample.
College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Da-Bing
DB
Ren
Yan-Hua
YH
Qin
Yong-Huan
YH
Yun
Hong-Mei
HM
Lu
Xiao-Qing
XQ
Chen
Yi-Zeng
YZ
Liang
D020001:1-Butanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D000431:Ethanol / Q000737:chemistry; D006586:Hexanes / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D008738:Methyl Ethers / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis*; D027544:Salvia / Q000737:chemistry*; D012997:Solvents; D014867:Water / Q000737:chemistry
D000085:Acetates; D004365:Drugs, Chinese Herbal; D006586:Hexanes; D008738:Methyl Ethers; D010936:Plant Extracts; D012997:Solvents; D014867:Water; C043243:methyl tert-butyl ether; C026385:n-hexane; D000431:Ethanol; C007650:ethyl acetate; D020001:1-Butanol; D000432:Methanol
Counter-current chromatography; Elution-extrusion; Recycling elution; Salvia plebeia R.Br; Traditional Chinese medicine