Terminal supraparticle assemblies from similarly charged protein molecules and nanoparticles.
Self-assembly of proteins and inorganic nanoparticles into terminal assemblies makes possible a large family of uniformly sized hybrid colloids. These particles can be compared in terms of utility, versatility and multifunctionality to other known types of terminal assemblies. They are simple to make and offer theoretical tools for designing their structure and function. To demonstrate such assemblies, we combine cadmium telluride nanoparticles with cytochrome C protein and observe spontaneous formation of spherical supraparticles with a narrow size distribution. Such self-limiting behaviour originates from the competition between electrostatic repulsion and non-covalent attractive interactions. Experimental variation of supraparticle diameters for several assembly conditions matches predictions obtained in simulations. Similar to micelles, supraparticles can incorporate other biological components as exemplified by incorporation of nitrate reductase. Tight packing of nanoscale components enables effective charge and exciton transport in supraparticles and bionic combination of properties as demonstrated by enzymatic nitrate reduction initiated by light absorption in the nanoparticle.
1] Departments of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA [2] [3].
Jai Il
JI
Park
1] Departments of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA [2].
Trung Dac
TD
Nguyen
Departments of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
Gleiciani
G
de Queirós Silveira
Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
Joong Hwan
JH
Bahng
Departments of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
Sudhanshu
S
Srivastava
Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA.
Gongpu
G
Zhao
Materials Science and Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
Kai
K
Sun
Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA.
Peijun
P
Zhang
1] Departments of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA [2] Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA [3] Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan 48019, USA.
Sharon C
SC
Glotzer
1] Departments of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA [2] Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA [3] Materials Science and Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA [4] Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan 48019, USA.
Nicholas A
NA
Kotov
D019187:Cadmium Compounds / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry* / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D062105:Molecular Docking Simulation; D050901:Nitrate Reductase / Q000378:metabolism; D010843:Pichia; D055672:Static Electricity; D013691:Tellurium / Q000737:chemistry*
D019187:Cadmium Compounds; D003102:Colloids; D045304:Cytochromes c; D050901:Nitrate Reductase; D013691:Tellurium; C028337:cadmium telluride