Application of silver nanoparticles to the chemiluminescence determination of cefditoren pivoxil using the luminol-ferricyanide system.
A new simple, accurate and sensitive sequential injection analysis chemiluminescence (CL) detection method for the determination of cefditoren pivoxil (CTP) has been developed. The developed method was based on the enhancement effect of silver nanoparticles on the CL signal arising from a luminol-potassium ferricyanide reaction in the presence of CTP. The optimum conditions relevant to the effect of luminol, potassium ferricyanide and silver nanoparticle concentrations were investigated. The proposed method showed linear relationships between relative CL intensity and the investigated drug concentration at the range 0.001-5000 ng/mL, (r = 0.9998, n = 12) with a detection limit of 0.5 pg/mL and quantification limit of 0.001 ng/mL. The relative standard deviation was 1.6%. The proposed method was employed for the determination of CTP in bulk drug, in its pharmaceutical dosage forms and biological fluids such as human serum and urine. The interference of some common additive compounds such as glucose, lactose, starch, talc and magnesium stearate was investigated. In addition, the interference of some related cephalosporins was tested. No interference was recorded. The obtained sequential injection analysis-CL results were statistically compared with those from a reported method and did not show any significant differences.
Department of Chemistry, College of Science, King Saud University, PO Box 22452, Riyadh, 11495, Saudi Arabia.
Nawal A
NA
Alarfaj
Fatma A
FA
Aly
Maha F
MF
El-Tohamy
D002511:Cephalosporins / Q000032:analysis*; D005292:Ferricyanides / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000379:methods; D006801:Humans; D049449:Luminescence; D008165:Luminol / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008968:Molecular Conformation; D012834:Silver / Q000737:chemistry*; D013607:Tablets / Q000737:chemistry
D002511:Cephalosporins; D005292:Ferricyanides; D013607:Tablets; C007931:hexacyanoferrate III; D012834:Silver; D008165:Luminol; C057710:cefditoren pivoxil
cefditoren pivoxil; chemiluminescence; luminol-ferricyanide system; sequential injection analysis; silver nanoparticles