Testosterone therapy differently regulates the anti- and pro-inflammatory cytokines in the plasma and prostate of rats submitted to chronic ethanol consumption (UChB).
OBJECTIVE
Ethanol consumption damages the prostate, and testosterone is known by anti-inflammatory role.


METHODS
The cytokines were investigated in the plasma and ventral prostate of UChB rats submitted or not to testosterone therapy by ELISA and Western blot, respectively. Additionally, inflammatory foci and mast cells were identified in the ventral prostate slides stained by hematoxylin and eosin and toluidine blue, respectively.


RESULTS
Inflammatory foci were found in the ethanol-treated animals and absent after testosterone therapy. Plasma levels of IL-6 and IL-10 were not changed while TNFα and TFG-β1 were increased in the animals submitted testosterone therapy. Regarding to ventral prostate, IL-6 did not alter, while IL-10, TNFα, and TFG-β1 were increased after testosterone therapy. Ethanol increases NFR2 in addition to high number of intact and degranulated mast cell which were reduced after testosterone therapy.


CONCLUSIONS
So, ethanol and testosterone differentially modulates the cytokines in the plasma and prostate.
Structural and Cell Biology Program, State University of Campinas - UNICAMP, Campinas/SP, Brazil.
Leonardo Oiveira
LO
Mendes
Wellerson Rodrigo
WR
Scarano
Sabrina Santos
SS
Rochel-Maia
Beatriz Aparecida
BA
Fioruci-Fontaneli
Luiz Gustavo Almeida
LG
Chuffa
Francisco Eduardo
FE
Martinez
D000428:Alcohol Drinking / Q000378:metabolism*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000276:immunology; D011467:Prostate / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051381:Rats; D013739:Testosterone / Q000494:pharmacology* / Q000627:therapeutic use
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D000431:Ethanol; D013739:Testosterone
Cytokines; ethanol; prostate; testosterone