Thermotolerant Bacillus kokeshiiformis sp. nov. isolated from marine animal resources compost.
A novel Gram-staining-positive, endospore-forming, rod-shaped, facultatively anaerobic, thermotolerant bacterium, designated strain MO-04(T), was isolated from a marine animal resources (MAR) compost. The 16S rRNA gene sequence of strain MO-04(T) showed 99.4% similarity with Bacillus thermolactis R-6488(T), 94.1% similarity with Bacillus thermoamylovorans CNCM I-1378(T), 93.3% similarity with Bacillus humi LMG 22167(T), 93.2% similarity with Bacillus niacini IFO 15566(T) and the similarities with other species were less than 93%. DNA-DNA relatedness between strain MO-04(T) and B. thermolactis DSM 23332(T) was 45%. The DNA G+C content of strain MO-04(T) was 33.4 mol%, comparatively lower than that of B. thermolactis R-6488(T) (35.0 mol%). Strain MO-04(T) grew at 35-61 °C (optimum 50 °C), pH 4.5-9.0 (optimum pH 7.2) and tolerated up to 8.0% (w/v) NaCl (optimum 2%). The MO-04(T) cell wall peptidoglycan type was meso-2,6-diaminopimelic acid, and the major fatty acids were C(16 : 1), C(14 : 1), C(17 : 0) and C(17 : 1). The major polar lipids were represented by diphosphatidylglycerol and phosphatidylglycerol and two unidentified phospholipids. The analysed polyphasic data presented here clearly indicate that the isolate MO-04(T) is considered to represent a novel species within the genus Bacillus for which the name Bacillus kokeshiiformis sp. nov. is proposed. The type strain of B. kokeshiiformis is MO-04(T) ( = JCM 19325(T) = KCTC 33163(T)).
Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Pramod
P
Poudel
Japan Eco-science Co. Ltd, 11-2 Shiomigaokacho, Chuo-ku, Chiba 260-0034, Japan.
Hirokuni
H
Miyamoto
Miroku Co. Ltd, 706-27, Mikoubou, Ohuchi, Kitsuki City, Oita 873-0006, Japan.
Hisashi
H
Miyamoto
Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Yuki
Y
Okugawa
Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Yukihiro
Y
Tashiro
Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Kenji
K
Sakai
D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005308:Fertilizers / Q000382:microbiology; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017747:Seafood; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D005308:Fertilizers; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid