Characterization of a lambda-cyhalothrin metabolizing glutathione S-transferase CpGSTd1 from Cydia pomonella (L.).
In insects, glutathione S-transferases (GSTs) are enzymes involved in detoxification of insecticides. However, few data are available for the codling moth, Cydia pomonella (L.). In this study, we cloned a delta class GST gene CpGSTd1 from C. pomonella. Real-time quantitative PCR shows that CpGSTd1 was up-regulated with aging, and the mRNA level of CpGSTd1 was higher in the fat body and silk glands than in other tissues. The expression level of CpGSTd1 exposure to insecticide suggests that CpGSTd1 is up-regulated after chlorpyrifos-methyl and lambda-cyhalothrin treatments. Both lambda-cyhalothrin and chlorpyrifos-methyl altered GST activity in vivo. The purified CpGSTd1 protein exhibits a high catalytic efficiency with CDNB and was inhibited by lambda-cyhalothrin and chlorpyrifos-methyl in vitro. Metabolism assays indicate that lambda-cyhalothrin was significantly metabolized while chlorpyrifos-methyl was not metabolized by CpGSTd1. Binding free energy analysis suggests that CpGSTd1 binding is tighter with lambda-cyhalothrin than with chlorpyrifos-methyl. Our study suggests that CpGSTd1 plays a key role in the metabolism of insecticides in C. pomonella.
Key Laboratory of Plant Protection Resources & Pest Management of Ministry of Education, College of Plant Protection, Northwest A & F University, Yangling, 712100, People's Republic of China.
Jiyuan
J
Liu
Xueqing
X
Yang
Yalin
Y
Zhang
D000818:Animals; D004390:Chlorpyrifos / Q000031:analogs & derivatives / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D007915:Lepidoptera / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D009570:Nitriles / Q000378:metabolism*; D011722:Pyrethrins / Q000378:metabolism*
D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D005982:Glutathione Transferase; D004390:Chlorpyrifos; C007031:chlorpyrifos-methyl; C037304:cyhalothrin