Validation of a food-frequency questionnaire assessment of methyl-group donors using estimated diet records and plasma biomarkers: the method of triads.
In this study, the intake of methyl-group donors (methionine, folate, betaine, and choline) among Flemish women of reproductive age (n = 30) assessed by a 7-d estimated diet record (7 d EDR) and food-frequency questionnaire (FFQ) was compared with plasma S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), and SAH:SAM ratio. Pearson correlation coefficients were calculated between each of the dietary methods and the validity coefficient was calculated using the method of triads. Correlations were higher between intake assessed by the FFQ and biomarkers than between 7 d EDR and biomarkers. The validity coefficients of the FFQ, when using SAH as a biomarker, were high (0.86 for methionine to 0.94 for folate), when the SAH:SAM ratio was used as a biomarker the validity coefficients ranged from 0.63 to 1.00. These data indicate that the FFQ is a reliable tool to estimate the intake of the methyl-group donors in women of reproductive age.
KU Leuven, Department of Public Health and Primary Care, Centre Environment & Health , Leuven , Belgium .
Sara
S
Pauwels
Ine
I
Doperé
Inge
I
Huybrechts
Lode
L
Godderis
Gudrun
G
Koppen
Greet
G
Vansant
D000293:Adolescent; D000328:Adult; D001530:Belgium; D001622:Betaine / Q000008:administration & dosage* / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002794:Choline / Q000008:administration & dosage* / Q000097:blood; D004032:Diet; D015930:Diet Records; D002149:Energy Intake; D005247:Feeding Behavior; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage* / Q000097:blood; D006801:Humans; D008715:Methionine / Q000008:administration & dosage* / Q000097:blood; D008745:Methylation; D008875:Middle Aged; D015596:Nutrition Assessment; D009752:Nutritional Status; D015203:Reproducibility of Results; D012435:S-Adenosylhomocysteine / Q000097:blood; D012436:S-Adenosylmethionine / Q000097:blood; D011795:Surveys and Questionnaires / Q000592:standards*; D055815:Young Adult
D015415:Biomarkers; D001622:Betaine; D012436:S-Adenosylmethionine; D005492:Folic Acid; D012435:S-Adenosylhomocysteine; D008715:Methionine; D002794:Choline
7 d EDR; FFQ; method of triads; methyl-group donors; validation