Stability of daptomycin 5 mg/mL and heparin sodium 100 units/mL combined in lactated Ringer's injection and stored in polypropylene syringes at 4 and -20°C.
OBJECTIVE
The stability of an admixture containing reconstituted daptomycin and heparin in lactated Ringer's injection was evaluated.


METHODS
Two samples of the admixture of daptomycin 5 mg/mL and heparin sodium 100 USP units/mL diluted in lactated Ringer's injection were prepared and divided into 5-mL portions for storage in syringes at 4 and -20 °C for 14 days. The percentage of the initial concentration of the drugs remaining in the syringes was assessed using a high-performance liquid chromatographic (HPLC) method with diode-array detection previously validated as stability indicating for both drugs. Forced degradation studies were performed independently with each drug diluted in lactated Ringer's injection. One sample from each stored syringe was analyzed in triplicate on days 0, 1, 2, 3, 4, 7, and 14; quality-control samples of each concentration tested were used throughout the analysis. The admixture samples were visually inspected for color, clarity, and the formation of particulate matter.


RESULTS
The HPLC analysis indicated no significant reduction (loss of ≤5%) in the concentration of daptomycin and heparin diluted in lactated Ringer's injection stored in syringes refrigerated at 4 °C and frozen at -20 °C. None of the chromatographic peaks observed in samples subjected to forced degradation were detected in any sample during the 14-day study. All of the syringe-stored samples remained clear and colorless on visual inspection for the duration of the study.


CONCLUSIONS
The admixture of daptomycin 5 mg/mL and heparin sodium 100 USP units/mL was stable when stored in polypropylene syringes for up to 14 days at 4 and -20 °C.
Raquel Ortega, Chemical.D., is Ph.D. student, Department of Analytical Chemistry, Faculty of Science, Granada, Spain. Antonio Salmerón-Garvía, Pharm.D., Ph.D., is Clinical Pharmacist, Hospital Pharmacy Unit, Baza Hospital, Granada. José Cabeza, Pharm.D., is Clinical Pharmacist and Head, Pharmacy Hospital Unit, University Hospital San Cecilio, Granada. Luís F. Capitán-Vallvey, Chemical.D., Ph.D., is Full Professor; and Natalia Navas, Chemical.D., Ph.D., is Professor, Department of Analytical Chemistry, Faculty of Science.
Raquel
R
Ortega
Raquel Ortega, Chemical.D., is Ph.D. student, Department of Analytical Chemistry, Faculty of Science, Granada, Spain. Antonio Salmerón-Garvía, Pharm.D., Ph.D., is Clinical Pharmacist, Hospital Pharmacy Unit, Baza Hospital, Granada. José Cabeza, Pharm.D., is Clinical Pharmacist and Head, Pharmacy Hospital Unit, University Hospital San Cecilio, Granada. Luís F. Capitán-Vallvey, Chemical.D., Ph.D., is Full Professor; and Natalia Navas, Chemical.D., Ph.D., is Professor, Department of Analytical Chemistry, Faculty of Science.
Antonio
A
Salmerón-García
Raquel Ortega, Chemical.D., is Ph.D. student, Department of Analytical Chemistry, Faculty of Science, Granada, Spain. Antonio Salmerón-Garvía, Pharm.D., Ph.D., is Clinical Pharmacist, Hospital Pharmacy Unit, Baza Hospital, Granada. José Cabeza, Pharm.D., is Clinical Pharmacist and Head, Pharmacy Hospital Unit, University Hospital San Cecilio, Granada. Luís F. Capitán-Vallvey, Chemical.D., Ph.D., is Full Professor; and Natalia Navas, Chemical.D., Ph.D., is Professor, Department of Analytical Chemistry, Faculty of Science.
José
J
Cabeza
Raquel Ortega, Chemical.D., is Ph.D. student, Department of Analytical Chemistry, Faculty of Science, Granada, Spain. Antonio Salmerón-Garvía, Pharm.D., Ph.D., is Clinical Pharmacist, Hospital Pharmacy Unit, Baza Hospital, Granada. José Cabeza, Pharm.D., is Clinical Pharmacist and Head, Pharmacy Hospital Unit, University Hospital San Cecilio, Granada. Luís F. Capitán-Vallvey, Chemical.D., Ph.D., is Full Professor; and Natalia Navas, Chemical.D., Ph.D., is Professor, Department of Analytical Chemistry, Faculty of Science.
Luís F
LF
Capitán-Vallvey
Raquel Ortega, Chemical.D., is Ph.D. student, Department of Analytical Chemistry, Faculty of Science, Granada, Spain. Antonio Salmerón-Garvía, Pharm.D., Ph.D., is Clinical Pharmacist, Hospital Pharmacy Unit, Baza Hospital, Granada. José Cabeza, Pharm.D., is Clinical Pharmacist and Head, Pharmacy Hospital Unit, University Hospital San Cecilio, Granada. Luís F. Capitán-Vallvey, Chemical.D., Ph.D., is Full Professor; and Natalia Navas, Chemical.D., Ph.D., is Professor, Department of Analytical Chemistry, Faculty of Science. natalia@ugr.es.
Natalia
N
Navas
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D017576:Daptomycin / Q000032:analysis / Q000737:chemistry*; D004355:Drug Stability; D006493:Heparin / Q000032:analysis / Q000737:chemistry*; D007267:Injections; D007552:Isotonic Solutions / Q000737:chemistry*; D011126:Polypropylenes; D000077325:Ringer's Lactate; D013594:Syringes; D013696:Temperature
D000900:Anti-Bacterial Agents; D007552:Isotonic Solutions; D011126:Polypropylenes; D000077325:Ringer's Lactate; D006493:Heparin; D017576:Daptomycin