Spectral reproducibility and quantification of peptides in MALDI of samples prepared by micro-spotting.
Previously, we reported that MALDI spectra of peptides became reproducible when temperature was kept constant. Linear calibration curves derived from such spectral data could be used for quantification. Homogeneity of samples was one of the requirements. Among the three popular matrices used in peptide MALDI [i.e., α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), and sinapinic acid (SA)], homogeneous samples could be prepared by conventional means only for CHCA. In this work, we showed that sample preparation by micro-spotting improved the homogeneity for all three cases.
Department of Chemistry, Seoul National University, Seoul, 151-747, Korea.
Yong Jin
YJ
Bae
Kyung Man
KM
Park
Sung Hee
SH
Ahn
Jeong Hee
JH
Moon
Myung Soo
MS
Kim
D053000:Analytic Sample Preparation Methods; D002138:Calibration; D003373:Coumaric Acids / Q000737:chemistry; D055664:Electrochemical Techniques; D005841:Gentisates / Q000737:chemistry; D057230:Limit of Detection; D008567:Membranes, Artificial; D008832:Microchemistry / Q000379:methods; D008970:Molecular Weight; D009842:Oligopeptides / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013499:Surface Properties
D003373:Coumaric Acids; D005841:Gentisates; D008567:Membranes, Artificial; D009842:Oligopeptides; C007175:alpha-cyano-4-hydroxycinnamate; C073734:sinapinic acid; C010925:2,5-dihydroxybenzoic acid