Mobilitalea sibirica gen. nov., sp. nov., a halotolerant polysaccharide-degrading bacterium.
A novel strictly anaerobic, halotolerant, organotrophic bacterium, strain P3M-3(T), was isolated from a microbial mat formed under the flow of hot water emerging from a 2775 m-deep well in Tomsk region (western Siberia, Russia). Cells of strain P3M-3(T) were straight and curved rods, 0.2-0.4 µm in width and 1.5-20 µm in length. Strain P3M-3(T) grew optimally at 37 °C, pH 7.0-7.5 and in a NaCl concentration of 15 g l(-1). Under optimum growth conditions, the doubling time was 1 h. The isolate was able to ferment a variety of mono-, di- and polysaccharides, including microcrystalline cellulose. Acetate, ethanol, H2 and CO2 were the main products of glucose fermentation. The DNA G+C content was 33.4 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain P3M-3(T) was a member of family Lachnospiraceae, whose representatives are also found in Clostridium cluster XIVa. 16S rRNA gene sequence similarity with Clostridium jejuense HY-35-12(T), the closest relative, was 93.9%. A novel genus and species, Mobilitalea sibirica gen. nov., sp. nov., are proposed based on phylogenetic analysis and physiological properties of the novel isolate. The type strain of the type species is P3M-3(T) ( = DSM 26468(T) = VKM B-2804(T)).
Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/2, 117312 Moscow, Russia.
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OA
Podosokorskaya
Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/2, 117312 Moscow, Russia.
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Bonch-Osmolovskaya
Gubkin Russian State University of Oil and Gas, Leninskiy Prospect 65, 119991 Moscow, Russia.
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Beskorovaynyy
Department of Genomic and Proteomic Research, Baltic Federal University, Botkina str. 3, 236016, Kaliningrad, Russia.
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SV
Toshchakov
Bioengineering Center, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/1, 117312 Moscow, Russia.
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TV
Kolganova
Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/2, 117312 Moscow, Russia.
I V
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Kublanov
D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005285:Fermentation; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D055049:Salt Tolerance; D017422:Sequence Analysis, DNA; D014871:Water Microbiology; D060986:Water Wells / Q000382:microbiology*
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S