Fermentation of lignocellulosic hydrolyzate using a submerged membrane bioreactor at high dilution rates.
A submerged membrane bioreactor (sMBR) was developed to ferment toxic lignocellulosic hydrolyzate to ethanol. The sMBR achieved high cell density of Saccharomyces cerevisiae during continuous cultivation of the hydrolyzate by completely retaining all yeast cells inside the sMBR. The performance of the sMBR was evaluated based on the ethanol yield and productivity at the dilution rates 0.2, 0.4, 0.6, and 0.8h(-1) with the increase of dilution rate. Results show that the yeast in the sMBR was able to ferment the wood hydrolyzate even at high dilution rates, attaining a maximum volumetric ethanol productivity of 7.94 ± 0.10 g L(-1)h(-1) at a dilution rate of 0.8h(-1). Ethanol yields were stable at 0.44 ± 0.02 g g(-1) during all the tested dilution rates, and the ethanol productivity increased from 2.16 ± 0.15 to 7.94 ± 0.10 g L(-1)h(-1). The developed sMBR systems running at high yeast density demonstrate a potential for a rapid and productive ethanol production from wood hydrolyzate.
Resource Recovery, University of Borås, Borås, Sweden; Industrial Biotechnology, Chalmers University of Technology, Göteborg, Sweden. Electronic address: paivi.ylitervo@hb.se.
Päivi
P
Ylitervo
Flemish Institute for Technological Research, Vito NV, Mol, Belgium. Electronic address: wim.doyen@vito.be.
Wim
W
Doyen
Resource Recovery, University of Borås, Borås, Sweden. Electronic address: mohammad.taherzadeh@hb.se.
Mohammad J
MJ
Taherzadeh
D000693:Anaerobiosis; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D008567:Membranes, Artificial; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D014934:Wood / Q000378:metabolism
D006601:Hexoses; D008567:Membranes, Artificial; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D005990:Glycerol
Bioethanol production; Cell retention; IPC membrane panel; Membrane bioreactor; Wood hydrolyzate