Phaseguide assisted liquid lamination for magnetic particle-based assays.
We have developed a magnetic particle-based assay platform in which functionalised magnetic particles are transferred sequentially through laminated volumes of reagents and washing buffers. Lamination of aqueous liquids is achieved via the use of phaseguide technology; microstructures that control the advancing air-liquid interface of solutions as they enter a microfluidic chamber. This allows manual filling of the device, eliminating the need for external pumping systems, and preparation of the system requires only a few minutes. Here, we apply the platform to two on-chip strategies: (i) a one-step streptavidin-biotin binding assay, and (ii) a two-step C-reactive protein immunoassay. With these, we demonstrate how condensing multiple reaction and washing processes into a single step significantly reduces procedural times, with both assay procedures requiring less than 8 seconds.
Department of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK. n.pamme@hull.ac.uk.
Chayakom
C
Phurimsak
Ender
E
Yildirim
Mark D
MD
Tarn
Sebastiaan J
SJ
Trietsch
Thomas
T
Hankemeier
Nicole
N
Pamme
Paul
P
Vulto
D001710:Biotin / Q000737:chemistry*; D002097:C-Reactive Protein / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D060526:Magnetic Fields; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D019809:Streptavidin / Q000737:chemistry*
D001710:Biotin; D002097:C-Reactive Protein; D019809:Streptavidin