MicroRNA-146a expression and its intervention in NF-кB signaling pathway in diabetic rat aorta.
OBJECTIVE
The present study was designed to evaluate whether microRNA-146a, as an NF-кB regulating factor and its adapter proteins (TRAF6 and IRAK1), are affected by diabetes in rat aorta.


METHODS
Male Wistar rats were randomly divided into control and diabetic groups (n=6 in each). Diabetes was induced by a single injection of streptozotocin (55 mg/kg; i.p.) in 12 h fasted rats. The gene expression of microRNA-146a, NF-κB, IRAK1, and TRAF6 were determined by real time PCR.


RESULTS
The expression of microRNA-146a was down-regulated in diabetic aorta when compared with the control group (p<0.05). The mRNA expression levels of NF-кB, TRAF6 and IRAK1 also increased in the diabetic rat aorta when compared with their control counterparts (p<0.01 for all comparisons).


CONCLUSIONS
These results suggest that down-regulation of microRNA-146a may lead to derangement in NF-кB negative feedback loop, propelling the aorta toward inflammation.
S S
SS
Emadi
F G
FG
Soufi
A M
AM
Khamaneh
M R
MR
Alipour
D000818:Animals; D001011:Aorta / Q000378:metabolism* / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D007249:Inflammation / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000235:genetics
C554180:MIRN146 microRNA, rat; D035683:MicroRNAs; D016328:NF-kappa B