Synthesis, biocompatible, and self-assembly properties of poly (ethylene glycol)/lactobionic acid-grafted chitosan.
Polymers with targeted ligands are widely used as the anti-cancer drug delivery materials. For applications of chitosan as an anti-liver cancer drug delivery, poly (ethylene glycol)/lactobionic acid-grafted chitosan (PEG/LA-CS) was prepared and investigated since lactobionic acid can be specifically recognized by the hepatocytes. The structure of the PEG/LA-CS was characterized by Fourier transform infrared spectrometry and elemental analysis. The self-assembly behaviors of the PEG/LA-CS were monitored by steady-state fluorescence spectroscopy and electronic transmission microscope. The protein adsorption of the PEG/LA-CS was detected with bovine serum albumin (BSA) by electrochemical impedance spectroscopy. The results showed that the PEG/LA-CS almost did not adsorb protein. To study the effects of PEG/LA-CS on the structure of BSA, the interactions between the PEG/LA-CS and BSA were detected by ultraviolet spectrum, fluorescence spectrum, and circular dichroism. All the data gave one result that BSA maintained its original folded confirmation in PEG/LA-CS solution. The hemocompatibility of PEG/LA-CS was investigated by observing the effects of PEG/LA-CS on the hemolysis rate and the plasma recalcification time (PRT). The results showed that the PRT was prolonged greatly and the hemolysis rate was less than 5%. Furthermore, PEG/LA-CS also showed good cytocompatibility with K562, Hep G2, and LO2 cells. Therefore, the PEG/LA-CS is believed to have great potential for producing injectable anti-liver cancer drug delivery.
Xiaoli
X
Song
Juan
J
Wang
Xiadan
X
Luo
Chunlan
C
Xu
Aiping
A
Zhu
Rong
R
Guo
Caifeng
C
Yan
Peizhi
P
Zhu
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity*; D001777:Blood Coagulation / Q000187:drug effects; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D048271:Chitosan / Q000737:chemistry*; D004187:Disaccharides / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D011092:Polyethylene Glycols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012995:Solubility
D001672:Biocompatible Materials; D004187:Disaccharides; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C005608:lactobionic acid; D048271:Chitosan
PEG/LA-CS; cytocompatibility; electrochemical impedance spectroscopy; hemocompatibility; self-assembly