Boseongicola aestuarii gen. nov., sp. nov., isolated from a tidal flat sediment.
A Gram-stain-negative, aerobic, non-motile and pleomorphic (coccoid, ovoid or rod-shaped) bacterial strain, BS-W15(T), isolated from a tidal flat sediment at Boseong in South Korea, was characterized taxonomically. Strain BS-W15(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of approximately 2.0% (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees, based on 16S rRNA gene sequences, revealed that strain BS-W15(T) joined the cluster comprising the type strains of Profundibacterium mesophilum, Hwanghaeicola aestuarii, M. pelagius and M. salinus, showing 93.5-96.4% sequence similarities. Strain BS-W15(T) contained Q-10 as the predominant ubiquinone and C(18 : 1)ω7c as the predominant fatty acid. The polar lipid profile of strain BS-W15(T) contained phosphatidylcholine and phosphatidylglycerol as major components, differentiating it from those of the type strains of P. mesophilum, H. aestuarii, M. pelagius and M. salinus. The DNA G+C content of strain BS-W15(T) was 58.7 mol%. The differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrate that strain BS-W15(T) is distinct from type strains of P. mesophilum, H. aestuarii, M. pelagius and M. salinus. On the basis of the data presented, strain BS-W15(T) is considered to represent a novel genus and species, for which the name Boseongicola aestuarii gen. nov., sp. nov. is proposed. The type strain is BS-W15(T) ( = KCTC 32576(T) = CECT 8489(T)).
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Sooyeon
S
Park
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Ja-Min
JM
Park
Microbiological Resource Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Keun-Chul
KC
Lee
Microbiological Resource Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Kyung Sook
KS
Bae
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Jung-Hoon
JH
Yoon
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010713:Phosphatidylcholines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2