Three-step photoconversion of only three subunits of the water-soluble chlorophyll-binding protein tetramer from Chenopodium album.
Water-soluble chlorophyll (Chl)-binding proteins (WSCPs) have been found in various plants. WSCPs are categorized into two classes based on their photoconvertibility: Class I (photoconvertible) and Class II (non-photoconvertible). Based on their absorption peaks, which occur in the red wavelengths, the pre- and post-photoconverted forms of Chenopodium album WSCP (CaWSCP) are called CP668 and CP742, respectively. Although various biochemical and biophysical properties of CaWSCP have already been characterized, questions remain regarding the structural dynamics of the photoconversion from CP668 to CP742, and the relationship between the photoconversion activity and incident light wavelength. To address how the wavelength of incident light affects the photoconversion, we performed time-course analyses of CaWSCP photoconversion by using light-emitting diodes that emit either white light, or at the discrete wavelengths 670, 645, 525, 470, or 430 nm. The most efficient photoconversion was observed under irradiation at 430 nm. Less efficient photoconversion was observed under irradiation with 670, 645, 470, or 525 nm light, in that order. The relationship between photoconversion activity and wavelength corresponded with the absorption peak intensities of Chls in the CaWSCP complex. The observed time dependence of the A(742)/A(668) ratio during photoconversion of the CaWSCP complex indicated that the photoconversion from CP668 to CP742 occurs in a three-step reaction, and that only three subunits in the complex could be photoconverted.
Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba, 274-8510, Japan.
Shigekazu
S
Takahashi
Akira
A
Uchida
Katsumi
K
Nakayama
Hiroyuki
H
Satoh
D027463:Chenopodium album; D061146:Chlorophyll Binding Proteins / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects*; D055668:Photochemical Processes; D021122:Protein Subunits / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects*
D061146:Chlorophyll Binding Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins