Micromonospora zeae sp. nov., a novel endophytic actinomycete isolated from corn root (Zea mays L.).
A novel actinomycete, designated strain NEAU-gq9(T), was isolated from corn root (Zea mays L.) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. On the basis of 16S rRNA gene sequence similarity studies, strain NEAU-gq9(T) was most closely related to Micromonospora zamorensis CR38(T) (99.3%), Micromonospora jinlongensis NEAU-GRX11(T) (99.2%), Micromonospora saelicesensis Lupac 09(T) (99.2%), Micromonospora chokoriensis 2-19(6)(T) (98.9%), Micromonospora coxensis 2-30-b(28)(T) (98.6%) and Micromonospora lupini Lupac 14N(T) (98.5%). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-gq9(T) is a member of the genus Micromonospora and supported the closest phylogenetic relationship to M. zamorensis CR38(T), M. jinlongensis NEAU-GRX11(T), M. saelicesensis Lupac 09(T), M. chokoriensis 2-19(6)(T) and M. lupini Lupac 14N(T). A combination of DNA-DNA hybridization, morphological and physiological characteristics indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. Therefore, it is proposed that strain NEAU-gq9(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora zeae sp. nov. is proposed. The type strain is NEAU-gq9(T) (=CGMCC 4.7092(T)=DSM 45882(T)).
1] College of Science, Northeast Agricultural University, Harbin, China [2] College of Agriculture, Northeast Agricultural University, Harbin, China.
Yue
Y
Shen
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, China.
Yuejing
Y
Zhang
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, China.
Chongxi
C
Liu
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, China.
Xiangjing
X
Wang
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, China.
Junwei
J
Zhao
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, China.
Feiyu
F
Jia
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, China.
Lingyu
L
Yang
College of Agriculture, Northeast Agricultural University, Harbin, China.
Deguang
D
Yang
1] Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, China [2] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Wensheng
W
Xiang
D004269:DNA, Bacterial / Q000235:genetics*; D008847:Micromonospora / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D003313:Zea mays / Q000382:microbiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S