The neuroprotective effects of carnosine in early stage of focal ischemia rodent model.
OBJECTIVE
This study was conducted to elucidate neuroprotective effect of carnosine in early stage of stroke.


METHODS
Early stage of rodent stroke model and neuroblastoma chemical hypoxia model was established by middle cerebral artery occlusion and antimycin A. Neuroprotective effect of carnosine was investigated with 100, 250, and 500 mg of carnosine treatment. And antioxidant expression was analyzed by enzyme linked immunosorbent assay (ELISA) and western blot in brain and blood.


RESULTS
Intraperitoneal injection of 500 mg carnosine induced significant decrease of infarct volume and expansion of penumbra (p<0.05). The expression of superoxide dismutase (SOD) showed significant increase than in saline group in blood and brain (p<0.05). In the analysis of chemical hypoxia, carnosine induced increase of neuronal cell viability and decrease of reactive oxygen species (ROS) production.


CONCLUSIONS
Carnosine has neuroprotective property which was related to antioxidant capacity in early stage of stroke. And, the oxidative stress should be considered one of major factor in early ischemic stroke.
Department of Neurosurgery, Seoul Medical Center, Seoul, Korea.
Hui-Seung
HS
Park
Research Institute, Seoul Medical Center, Seoul, Korea.
Kyung-Hoon
KH
Han
Research Institute, Seoul Medical Center, Seoul, Korea.
Jeoung-A
JA
Shin
Research Institute, Seoul Medical Center, Seoul, Korea.
Joo-Hyun
JH
Park
Department of Neurosurgery, Seoul Medical Center, Seoul, Korea.
Kwan-Young
KY
Song
Research Institute, Seoul Medical Center, Seoul, Korea.
Doh-Hee
DH
Kim
Carnosine; Early stage; Focal ischemia; Neuroprotective effect