Piperlongumine as a potential activator of AMP-activated protein kinase in HepG2 cells.
AMP-activated protein kinase (AMPK) is a key regulator of fatty acid biosynthesis and fatty acid oxidation throughout the body. Piperlongumine (PL) isolated from Piper longum (L.) was shown to potently upregulate activation of AMPK via phosphorylation and inactivation of acetyl-CoA carboxylases in cultured HepG2 cells, presumably enhancing the transfer of fatty acids into mitochondrial cells by inhibiting malonyl-CoA production. PL showed cytotoxicity on HepG2 cell growth at the concentration of 5 μM of PL, while more than 80% of HepG2 cells were survived at the concentration of 2 μM of PL. Overall, the results of this study indicate that PL activates AMPK phosphorylation and possesses cytotoxicity in HepG2 cells.
a School of Life Science and Biotechnology, Kyungpook National University , Daegu 702-701 , Korea.
Jahee
J
Ryu
Myoung-Jin
MJ
Kim
Tae-Oh
TO
Kim
Tae-Lin
TL
Huh
Sung-Eun
SE
Lee
D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000103:Acetyl-CoA Carboxylase; D004148:Dioxolanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D008316:Malonyl Coenzyme A; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010766:Phosphorylation; D031701:Piper / Q000737:chemistry*; D010881:Piperidones
D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase; D004148:Dioxolanes; D005227:Fatty Acids; D008316:Malonyl Coenzyme A; C498077:piperlongumine; D010881:Piperidones
AMP-activated protein kinase; HepG2 cell; phosphorylation; piperlongumine