Neutrophils release extracellular DNA traps in response to exercise.
Intense exercise evokes a rapid and transient increase in circulating cell-free DNA (cf-DNA), a phenomenon that is commonly observed in a variety of acute and chronic inflammatory conditions. In this study, we aimed to shed new light on the release and clearance mechanisms of cf-DNA in response to exercise. We hypothesized that activated neutrophils may primarily contribute to exercise-evoked cf-DNA levels by releasing neutrophil extracellular traps (NETs). Analysis of plasma and/or serum samples from male athletes at rest and in response to exhaustive treadmill exercise revealed an immediate and transient increase in cf-DNA that was concomitantly counterbalanced by an increase in serum DNase activity. Consistently, rapid release and clearance kinetics for cf-DNA could also be observed in response to intensive cycling exercise, with no significant differences between endurance-trained (V̇o2max >57 ml·min(-1)·kg(-1)) and healthy (V̇o2max <49 ml·min(-1)·kg(-1)) sedentary individuals. In postexercise blood smear samples, we detected seemingly intact neutrophils displaying morphological signs of NET release, as indicated by abnormal swollen nuclei and emanating DNA fibers. In support, we observed a striking correlation of postexercise cf-DNA concentrations with plasma levels of the granule-derived enzyme myeloperoxidase. Our study indicates that intense exercise induces liberation of NETs, which is sufficiently counterbalanced in healthy individuals by a concomitant rise in serum DNase activity. As aberrant release of NETs has been linked to diverse disease states, monitoring of cf-DNA/DNase levels or activities in response to standardized exercise testing could provide a valuable tool to identify people who are at increased risk for cardiac ischemia, thrombosis, autoimmunity, or chronic fatigue.
Department of Sports Medicine, Medical Clinic, Eberhard-Karls-University of Tuebingen; thomas.beiter@med.uni-tuebingen.de.
Thomas
T
Beiter
Department of Sports Medicine, Medical Clinic, Eberhard-Karls-University of Tuebingen;
Annunziata
A
Fragasso
Department of Sports Medicine, Medical Clinic, Eberhard-Karls-University of Tuebingen;
Jens
J
Hudemann
Department of Sports Medicine, Justus-Liebig University, Giessen; and.
Marius
M
Schild
Division of Sports and Rehabilitation Medicine, Ulm University Medical Center, Germany.
Jürgen
J
Steinacker
Department of Sports Medicine, Justus-Liebig University, Giessen; and.
Frank C
FC
Mooren
Department of Sports Medicine, Medical Clinic, Eberhard-Karls-University of Tuebingen;
Andreas M
AM
Niess
D000328:Adult; D004247:DNA / Q000097:blood / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test / Q000379:methods; D065206:Extracellular Traps / Q000502:physiology*; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D055815:Young Adult
D004247:DNA; D009195:Peroxidase; D003851:Deoxyribonucleases
DNase; cell-free DNA; neutrophil extracellular traps