sHLA-G involved in the apoptosis of decidual natural killer cells following Toxoplasma gondii infection.
This study aims to assess whether soluble HLA-G (sHLA-G) is involved in apoptosis of decidual natural killer (dNK) cells following Toxoplasma gondii infection. dNK cells or NK-92 cells were infected with T. gondii and co-cultured with trophoblast cells or BeWo cells. Infected co-cultured cells were treated without or with sHLA-G neutralizing antibody. Uninfected co-cultured cells were used as controls. Apoptosis of dNK cells were analyzed by flow cytometry and confocal microscope. Real-time PCR and Western blot were used to determine caspase 3 and caspase 8 expression. sHLA-G in supernatant were measured by enzyme-linked immunosorbent assay (ELISA). In infection groups, sHLA-G was increased, while dNK apoptosis proteins caspase 3 and caspase 8 were up-regulated, but significantly decreased in the presence of sHLA-G neutralizing antibody compared to controls. Under the situation of T. gondii-infected dNK cells co-cultured with trophoblast cells, the up-regulation of sHLA-G could induce dNK cells apoptosis which ultimately may contribute to the abnormal pregnancy outcomes with T. gondii infection.
Department of Reproductive Medicine, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China, 256603.
Meiyu
M
Han
Yuzhu
Y
Jiang
Kaixue
K
Lao
Xiaoyan
X
Xu
Shaowei
S
Zhan
Yanlin
Y
Wang
Xuemei
X
Hu
D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018920:Coculture Techniques; D003656:Decidua / Q000378:metabolism* / Q000382:microbiology; D005260:Female; D059951:HLA-G Antigens / Q000096:biosynthesis*; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism* / Q000382:microbiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000378:metabolism*; D014123:Toxoplasmosis / Q000378:metabolism*
D059951:HLA-G Antigens