High-throughput and automatic typing via human papillomavirus identification map for cervical cancer screening and prognosis.
A novel human papillomavirus (HPV) typing assay for cervical cancer screening and prognosis was developed by the combination of restriction fragment length polymorphism (RFLP) and microchip electrophoresis (MCE) to achieve higher levels of sensitivity and throughput. The detection limit of 2 × 10(2) copies, high sensitivity and typing accuracy on the account of PCR-RFLP-MCE method guarantee the successful diagnosis results of 4-fold higher infection rate over cytologic tests. From clinical samples, eleven kinds of HPV types were identified with a good compatibility degree of over 90%. The described method showed good reliability in clinical samples and provided a promising alternative for pathological studies at the molecular level.
College of Chemistry, Fuzhou University, Fuzhou, 350108, China. xxq@fzu.edu.cn.
Linglu
L
Yi
Xueqin
X
Xu
Xuexia
X
Lin
Haifang
H
Li
Yuan
Y
Ma
Jin-Ming
JM
Lin
D001483:Base Sequence; D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D055088:Early Detection of Cancer; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D004867:Equipment Design; D005260:Female; D060005:Genotyping Techniques / Q000295:instrumentation; D057166:High-Throughput Screening Assays / Q000295:instrumentation; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D015203:Reproducibility of Results; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000209:etiology / Q000821:virology*
D004279:DNA, Viral