A novel DAD type and folic acid conjugated fluorescent monomer as a targeting probe for imaging of folate receptor overexpressed cells.
We describe a modification and post-functionalization technique for a donor-acceptor-donor type monomer; 6-(4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-2H-benzo[d][1,2, 3]triazol-2-yl)hexan-1-amine. Folic acid was attached to the fluorescent structure. The conjugation was confirmed via NMR and Fourier transform infrared analyses. Cytotoxicity was investigated and the comparison of association of targeted monomeric structures in tumor cells was monitored via fluorescence microscopy.
Dept. of Biotechnology, Middle East Technical University, Ankara, 06800, Turkey.
Fulya
F
Ekiz Kanik
Didem
D
Ag
Muharrem
M
Seleci
Firat Baris
FB
Barlas
Melis
M
Kesik
Gonul
G
Hizalan
Hava
H
Akpinar
Suna
S
Timur
Levent
L
Toppare
D003952:Diagnostic Imaging; D005456:Fluorescent Dyes / Q000737:chemistry*; D058975:Folate Receptor 1 / Q000096:biosynthesis / Q000302:isolation & purification*; D005492:Folic Acid / Q000031:analogs & derivatives* / Q000737:chemistry*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014230:Triazoles / Q000737:chemistry*
D005456:Fluorescent Dyes; D058975:Folate Receptor 1; D014230:Triazoles; C000596023:folic aid-6-(4,7-bis(2,3-dihydrothieno(3,4-b)(1,4)dioxin-5-yl)-2H-benzo(d)(1,2, 3)triazol-2-yl)hexan-1-amine; D005492:Folic Acid
cell imaging; conjugation; fluorescence microscopy; folic acid