Tertiary-amine functionalized polyplexes enhanced cellular uptake and prolonged gene expression.
Ultrasound (US) has been found to facilitate the transport of DNA across cell membranes. However, the transfection efficiency is generally low, and the expression duration of the transfected gene is brief. In this study, a tertiary polycation, Poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA), was used as a carrier for US-mediated gene transfection. Its in-vitro and in-vivo effects on the transfection efficiency and the expression duration were evaluated. A mixture of pCI-neo-luc and PDMAEMA was transfected to cultured cells or mouse muscle by exposure to 1-MHz pulse US. A strong expression of luciferase was found 10 days after the transfection in vitro regardless of US exposure. However, effective transfection only occurred in the US groups in vivo. The transfection ability depended on the weight ratio of PDMAEMA to DNA, and was different for the in-vitro and in-vivo conditions. Lower weight ratios, e.g., 0.25, exhibited better in-vivo expression for at least 45 days.
Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, ROC.
Chia-Wen
CW
Lo
R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Jhong-Li, Taoyuan, Taiwan, ROC.
Yung
Y
Chang
Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, ROC.
Jyun-Lin
JL
Lee
Department of Chemical Engineering, National Taiwan University College of Medicine, Taipei, Taiwan, ROC.
Wei-Bor
WB
Tsai
Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, ROC.
Wen-Shiang
WS
Chen
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004337:Drug Carriers; D015870:Gene Expression; D017930:Genes, Reporter; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D009757:Nylons / Q000737:chemistry / Q000494:pharmacology*; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D011094:Polyethyleneimine / Q000494:pharmacology; D013016:Sound; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
D004337:Drug Carriers; D008689:Methacrylates; D009757:Nylons; C407037:poly(2-(dimethylamino)ethyl methacrylate); D011094:Polyethyleneimine; D004247:DNA; D008156:Luciferases