A Versatile Tool for Stable Inhibition of microRNA Activity.
MicroRNAs (miRNAs) are a class of small RNAs (18-22 nt) that post transcriptionally regulate gene expression by binding to complementary sequences on target mRNAs, resulting in translational repression or target degradation and gene silencing. As aberrant expression of miRNAs is implicated in important diseases including cancer miRNA-based therapies are under intensive investigation. We optimized strategies to stably or conditionally generate miRNA inhibitors for a continuous block of miRNA activity that allows for probing miRNA function in long-term cell culture experiments, cancer xenografts, 3D tissue models and for in vivo studies with transgenic organisms.
Institute of Biomedical Technologies, National Research Council, 20090 Segrate-Milan, Italy. paride.pelucchi@itb.cnr.it.
Paride
P
Pelucchi
Institute of Biomedical Technologies, National Research Council, 20090 Segrate-Milan, Italy. valeria.tria@itb.cnr.it.
Valeria
V
Tria
Institute of Biomedical Technologies, National Research Council, 20090 Segrate-Milan, Italy. valentina.martino@itb.cnr.it.
Valentina
V
Martino
Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, Robert-Kock Str. 39, D-50931, Cologne, Germany. dsabour@uni-koeln.de.
Davood
D
Sabour
Pathology and Anatomy Unit, Manerbio, Desenzano d/G, 25025 Manerbio, Italy. g.bertalot@libero.it.
Giovanni
G
Bertalot
Institute of Biomedical Technologies, National Research Council, 20090 Segrate-Milan, Italy. stefano.molgora@itb.cnr.it.
Stefano
S
Molgora
Institute of Biomedical Technologies, National Research Council, 20090 Segrate-Milan, Italy. mira.palizban@uni-muenster.de.
Mira
M
Palizban
University of Münster Medical Center, Gynecology and Obstetrics, Münster 48149, Germany. martingotte@uni-muenster.de.
Martin
M
Götte
Institute of Biomedical Technologies, National Research Council, 20090 Segrate-Milan, Italy. ileana.zucchi@itb.cnr.it.
Ileana
I
Zucchi
Institute of Biomedical Technologies, National Research Council, 20090 Segrate-Milan, Italy. rreinbold@icloud.com.
Rolland A
RA
Reinbold