Improved gene transfer with histidine-functionalized mesoporous silica nanoparticles.
Mesoporous silica nanoparticles (MSN) were functionalized with aminopropyltriethoxysilane (MSN-NH2) then L-histidine (MSN-His) for pDNA delivery in cells and in vivo. The complexation of pDNA with MSN-NH2 and MSN-His was first studied with gel shift assay. pDNA complexed with MSN-His was better protected from DNase degradation than with MSN-NH2. An improvement of the transfection efficiency in cells was observed with MSN-His/pDNA compared to MSN-NH2/pDNA, which could be explained by a better internalization of MSN-His. The improvement of the transfection efficiency with MSN-His was also observed for gene transfer in Achilles tendons in vivo.
Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-UM2-ENSCM-UM1, CC1701 Equipe Chimie Moléculaire et Organisation du Solide, Place Eugène Bataillon, Cedex 05, Montpellier 34095, France.
David
D
Brevet
Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-UM2-ENSCM-UM1, CC1701 Equipe Chimie Moléculaire et Organisation du Solide, Place Eugène Bataillon, Cedex 05, Montpellier 34095, France.
Ouahiba
O
Hocine
Centre de Biophysique Moléculaire, CNRS-UPR 4301, rue Charles Sadron, Orléans 45071, France.
Anthony
A
Delalande
Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-UM2-ENSCM-UM1, CC1701 Equipe Chimie Moléculaire et Organisation du Solide, Place Eugène Bataillon, Cedex 05, Montpellier 34095, France.
Laurence
L
Raehm
Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-UM2-ENSCM-UM1, CC1701 Equipe Chimie Moléculaire et Organisation du Solide, Place Eugène Bataillon, Cedex 05, Montpellier 34095, France.
Clarence
C
Charnay
Centre de Biophysique Moléculaire, CNRS-UPR 4301, rue Charles Sadron, Orléans 45071, France.
Patrick
P
Midoux
Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-UM2-ENSCM-UM1, CC1701 Equipe Chimie Moléculaire et Organisation du Solide, Place Eugène Bataillon, Cedex 05, Montpellier 34095, France. Electronic address: durand@um2.fr.
Jean-Olivier
JO
Durand
Centre de Biophysique Moléculaire, CNRS-UPR 4301, rue Charles Sadron, Orléans 45071, France. Electronic address: chantal.pichon@cnrs-orleans.fr.
Chantal
C
Pichon
D000125:Achilles Tendon / Q000378:metabolism; D000818:Animals; D003587:Cytomegalovirus / Q000235:genetics; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D057809:HEK293 Cells; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D049409:Luciferases, Firefly / Q000235:genetics; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010957:Plasmids; D011437:Propylamines; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D014162:Transfection
D004337:Drug Carriers; D011437:Propylamines; D012821:Silanes; D006639:Histidine; D012822:Silicon Dioxide; D004247:DNA; D049409:Luciferases, Firefly; C477625:amino-propyl-triethoxysilane
Histidine; Mesoporous silica nanoparticles; Nucleic acid delivery; Transfection in vivo