MicroRNA-26a-5p and microRNA-23b-3p up-regulate peroxiredoxin III in acute myeloid leukemia.
MicroRNAs (miRNAs) are small RNAs that regulate target gene expression. Using microarray-based miRNA expression profiling, we compared the miRNA expression in granulocytes from four patients with acute myeloid leukemia and four healthy controls. Thirty-four miRNAs were found to be differentially expressed, including 20 miRNAs that were up-regulated and 14 miRNAs that were down-regulated. The expression of selected miRNAs (miR-26a-5p and miR-23b-3p) was independently validated in 20 patients and 12 healthy controls. Notably, we demonstrated that peroxiredoxin III (PrxIII) is a common direct target of both miR-26a-5p and miR-23b-3p. Furthermore, these results indicate that the two decreased miRNAs could scavenge cellular reactive oxygen species (ROS) by targeting the PrxIII gene. These findings are discussed with regard to the known function of PrxIII as a ROS scavenger and the high endogenous ROS levels required for hematopoietic stem cell differentiation. These findings may potentially offer insights into the pathological relationships between miR-26a-5p, miR-23b-3p and leukemogenesis.
Key Laboratory of Experimental Teratology, Ministry of Education, Institute of Medical Genetics, School of Medicine, Shandong University , Jinan, Shandong , China.
Wenjie
W
Jiang
Jie
J
Min
Xiaohui
X
Sui
Yanyan
Y
Qian
Ying
Y
Liu
Zhaojian
Z
Liu
Haibin
H
Zhou
Xi
X
Li
Yaoqin
Y
Gong
D020413:3' Untranslated Regions / Q000235:genetics; D000208:Acute Disease; D000328:Adult; D002454:Cell Differentiation / Q000235:genetics; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006098:Granulocytes / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D020014:K562 Cells; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D061105:Peroxiredoxin III / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome; D015854:Up-Regulation
D020413:3' Untranslated Regions; D035683:MicroRNAs; D061105:Peroxiredoxin III; D017382:Reactive Oxygen Species; C552430:MIRN23a microRNA, human; C528163:MIRN26 microRNA, human
AML; PrxIII; miR-23b-3p; miR-26a-5p