Mechanical properties of mouse lungs along organ decellularization by sodium dodecyl sulfate.
Lung decellularization is based on the use of physical, chemical, or enzymatic methods to break down the integrity of the cells followed by a treatment to extract the cellular material from the lung scaffold. The aim of this study was to characterize the mechanical changes throughout the different steps of lung decellularization process. Four lungs from mice (C57BL/6) were decellularized by using a conventional protocol based on sodium dodecyl sulfate. Lungs resistance (R(L)) and elastance (E(L)) were measured along decellularization steps and were computed by linear regression fitting of tracheal pressure, flow, and volume during mechanical ventilation. Transients differences found were more distinct in an intermediate step after the lungs were rinsed with deionized water and treated with 1% SDS, whereupon the percentage of variation reached approximately 80% for resistance values and 30% for elastance values. In conclusion, although a variation in extracellular matrix stiffness was observed during the decellularization process, this variation can be considered negligible overall because the resistance and elastance returned to basal values at the final decellularization step.
Unitat de Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Spain; Master's and Doctoral Degree Programs in Rehabilitation Sciences, Nove de Julho University, Sao Paulo, Brazil.
Paula N
PN
Nonaka
Unitat de Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Spain; CIBER de Enfermedades Respiratorias, Bunyola, Spain.
Juan J
JJ
Uriarte
Unitat de Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Spain; CIBER de Enfermedades Respiratorias, Bunyola, Spain; Institut de Bioenginyeria de Catalunya, Barcelona, Spain.
Noelia
N
Campillo
Unitat de Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Spain; CIBER de Enfermedades Respiratorias, Bunyola, Spain.
Esther
E
Melo
Unitat de Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Spain; CIBER de Enfermedades Respiratorias, Bunyola, Spain; Institut de Bioenginyeria de Catalunya, Barcelona, Spain.
Daniel
D
Navajas
Unitat de Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Spain; CIBER de Enfermedades Respiratorias, Bunyola, Spain.
Ramon
R
Farré
Master's and Doctoral Degree Programs in Rehabilitation Sciences, Nove de Julho University, Sao Paulo, Brazil. Electronic address: oliveira.lvf@uninove.br.
Luis V F
LV
Oliveira
D000403:Airway Resistance; D000818:Animals; D003902:Detergents; D004548:Elasticity; D005109:Extracellular Matrix / Q000502:physiology*; D005260:Female; D005615:Freezing; D007211:Indoles; D016014:Linear Models; D008168:Lung / Q000166:cytology* / Q000502:physiology*; D008810:Mice, Inbred C57BL; D009929:Organ Size; D011312:Pressure; D012121:Respiration, Artificial; D015656:Respiratory Mechanics; D012967:Sodium Dodecyl Sulfate; D014132:Trachea / Q000502:physiology
D003902:Detergents; D007211:Indoles; D012967:Sodium Dodecyl Sulfate; C007293:DAPI
Lung bioengineering; Lung decellularization; Organ scaffold