CD 10 expression intensity in various grades and stages of urothelial carcinoma of urinary bladder.
OBJECTIVE
To evaluate CD10 expression in urothelial carcinoma of the urinary bladder and the association of immunohistochemical (IHC) CD10 expression intensity with grade and stage.


METHODS
Descriptive cross-sectional analytical study.


METHODS
Armed Forces Institute of Pathology, Rawalpindi, from January to December 2011.


METHODS
Fifty consecutive cases of urothelial bladder carcinomas, obtained through transurethral resections, were included in this study. Hematoxylin-eosin (HE) stained sections from each case were re-evaluated histopathologically according to WHO 2004 grading system. The TNM system was used for pathologic staging. On selected slides IHC CD10 marker was applied and a semiquantitative scoring for its expression based on the percentage of positive cells and intensity was performed. Data was entered and analysed on SPSS version 17. Fisher's exact test was used to compare grades, stages of urothelial carcinoma with CD 10 expression and age groups. P < 0.05 was taken as level of significance.


RESULTS
Urothelial carcinoma was more common in males. The male to female ratio was 9:1. The older patients > 50 years had higher grade and stage as compared to the younger patients. All cases of high grade urothelial carcinoma showed higher positivity for CD 10. Twenty cases (86.95%) of high grade urothelial carcinoma were positive with +2 immunostaining while 3 cases (13.04 %) were positive with +1 staining. None of the tumors of stage pTa was positive for CD 10 expression. Of all patients with stage pT 1 tumor, 1 case (5.3%) was CD 10 negative and 17 cases (89.9%) were CD 10 positive having +1 staining with 5 - 50% staining and 1 case (5.3%) had +2 staining with more then 50% expression. Out of all patients with stage pT 2, no tumor was CD 10 negative, 3 (13.6%) patients were CD 10 positive with +1 staining and 19 (86.4%) with stage pT 2 tumor had stained positive with +2 staining.


CONCLUSIONS
CD 10 expression was greater in high grade and invasive urothelial carcinomas; it may be associated with tumor progression in bladder cancer pathogenesis.
Department of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi.
Muhammad
M
Atique
Department of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi.
Muhammad Sajjad
MS
Abbasi
Department of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi.
Shahid
S
Jamal
Department of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi.
Muhammad Tahir
MT
Khadim
Department of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi.
Farhan
F
Akhtar
Department of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi.
Nighat
N
Jamal
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology*; D003430:Cross-Sectional Studies; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D015260:Neprilysin / Q000378:metabolism*; D001743:Urinary Bladder / Q000166:cytology / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology*; D019459:Urothelium / Q000473:pathology
D014408:Biomarkers, Tumor; D015260:Neprilysin