Phenotypic screening and molecular analysis of blast resistance in fragrant rice for marker assisted selection.
Experiments were conducted to identify blast-resistant fragrant genotypes for the development of a durable blast-resistant rice variety during years 2012-2013. The results indicate that out of 140 test materials including 114 fragrant germplasms, 25 differential varieties (DVs) harbouring 23 blast-resistant genes, only 16 fragrant rice germplasms showed comparatively better performance against a virulent isolate of blast disease. The reaction pattern of single-spore isolate of Magnaporthe oryzae to differential varieties showed that Pish, Pi9, Pita-2 and Pita are the effective blast-resistant genes against the tested blast isolates in Bangladesh. The DNA markers profiles of selected 16 rice germplasms indicated that genotype Chinigura contained Pish, Pi9 and Pita genes; on the other hand, both BRRI dhan50 and Bawaibhog contained Pish and Pita genes in their genetic background. Genotypes Jirakatari, BR5, and Gopalbhog possessed Pish gene, while Uknimodhu, Deshikatari, Radhunipagol, Kalijira (3), Chinikanai each contained the Pita gene only. There are some materials that did not contain any target gene(s) in their genetic background, but proved resistant in pathogenicity tests. This information provided valuable genetic information for breeders to develop durable blast-resistant fragrant or aromatic rice varieties in Bangladesh.
Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.
Mohammad Ashik Iqbal
MA
Khan
Department of Genetics and Plant Breeding, Patuakhali Science and Technology University, Patuakhali 8602, Bangladesh.
Partha Pratim
PP
Sen
Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.
Rejwan
R
Bhuiyan
Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.
Enamul
E
Kabir
Department of Genetics and Plant Breeding, Patuakhali Science and Technology University, Patuakhali 8602, Bangladesh.
Abul Kashem
AK
Chowdhury
Japan International Research Center for Agricultural Sciences, 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan.
Yoshimichi
Y
Fukuta
Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.
Ansar
A
Ali
Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh; Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Sealngor, Malaysia. Electronic address: alatif1965@yahoo.com.
Mohammad Abdul
MA
Latif
D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics* / Q000502:physiology*; D017343:Genes, Plant / Q000235:genetics; D005819:Genetic Markers; D020082:Magnaporthe / Q000502:physiology; D009181:Mycoses / Q000382:microbiology*; D012275:Oryza / Q000235:genetics* / Q000502:physiology*; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D013045:Species Specificity
D018744:DNA, Plant; D005819:Genetic Markers
Blast disease; DNA markers; Fragrant rice; Pathogenicity; Resistance