Effects of the acute exposition to glyphosate-based herbicide on oxidative stress parameters and antioxidant responses in a hybrid Amazon fish surubim (Pseudoplatystoma sp).
The aim of this study was to investigate the effects of acute glyphosate (active ingredient) exposure on the oxidative stress biomarkers and antioxidant defenses of a hybrid surubim (Pseudoplatystoma sp). The fish were exposed to different herbicide concentrations for 96 h. The thiobarbituric acid-reactive substances (TBARS), protein carbonyls and antioxidant responses were verified. The 15 mg a.pL(-1) of herbicide resulted in the death of 50% of the fish after 96 h. An increase in liver and muscle TBARS levels was observed when fish were exposed to the herbicide. The protein carbonyl content was also increased in the liver (4.5mg a.pL(-1) concentration) and brain (2.25 mg a.pL(-1) concentration). The antioxidant activities decreased in the liver and brain after exposure to herbicide. Levels of ascorbic acid in the liver (2.25 mg a.pL(-1) and 4.5 mg a.pL(-1) concentrations) and brain (2.25 mg a.pL(-1) concentration) were increased post-treatment. Levels of total thiols were increased in the liver and brain (2.25 mg L(-1) and 7.5mg a.pL(-1), respectively). Glyphosate exposure, at the tested concentrations affects surubim health by promoting changes that can affect their survival in natural environment. Some parameters as TBARS and protein carbonyl could be early biomarkers for Roundup exposure in this fish species.
Instituto de Ciências Naturais, Humanas e Sociais/Laboratórios Integrados de Pesquisas Químicas, Universidade Federal de Mato Grosso (UFMT), Câmpus de Sinop, MT, Brazil.
Valéria Dornelles Gindri
VD
Sinhorin
Instituto de Ciências Naturais, Humanas e Sociais/Laboratórios Integrados de Pesquisas Químicas, Universidade Federal de Mato Grosso (UFMT), Câmpus de Sinop, MT, Brazil.
Adilson Paulo
AP
Sinhorin
Instituto de Ciências da Saúde, Universidade Federal de Mato Grosso (UFMT), Câmpus de Sinop, MT, Brazil.
Jhonnes Marcos dos Santos
JM
Teixeira
Instituto de Ciências da Saúde, Universidade Federal de Mato Grosso (UFMT), Câmpus de Sinop, MT, Brazil.
Kelly Márcia Lazarotto
KM
Miléski
Instituto de Ciências da Saúde, Universidade Federal de Mato Grosso (UFMT), Câmpus de Sinop, MT, Brazil.
Paula Carine
PC
Hansen
Instituto de Ciências Agrárias e Ambientais, Universidade Federal de Mato Grosso (UFMT), Câmpus de Sinop, MT, Brazil.
Paula Sueli Andrade
PS
Moreira
Instituto de Ciências Exatas e da Terra, UFMT, Câmpus de Cuiabá, MT, Brazil.
Nair Honda
NH
Kawashita
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Campus de Araraquara, SP, Brazil.
Amanda Martins
AM
Baviera
Laboratório de Bioquímica Adaptativa e Toxicologia de Peixes, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. Electronic address: vania.loro@gmail.com.
Vania Lúcia
VL
Loro
D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis; D015415:Biomarkers / Q000378:metabolism; D002397:Catfishes / Q000502:physiology*; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D009132:Muscles / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000097797:Glyphosate
D001205:Ascorbic Acid; D015415:Biomarkers; D005998:Glycine; D006540:Herbicides; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D000097797:Glyphosate
Fish farmers; Hybrid Amazon fish; Oxidative stress; Pesticide; Roundup; Soybean monoculture