Biofunctionalization of PEGylated microcapsules for exclusive binding to protein substrates.
Targeted delivery of drugs to specific diseased sites in the body is an area of research that has attracted many studies, particularly in drug deliveries that utilize microparticles. By achieving targeted delivery of a drug, one can increase the efficacy of the treatment, thus, reducing unwanted side effects. This study aims to synthesize microcapsules that are able to target and adsorb to specific proteins (i.e., collagen type IV and fibronectin) through antibody-antigen interactions, while simultaneously suppressing any unspecific binding, a characteristic that is commonly observed in polyelectrolyte microcapsule-protein interactions. This is accomplished by creating an antibody-functionalized poly(ethylene glycol) (PEG) assembly within the microcapsule structure. Site-specific adsorption of these microcapsules is tested using protein micropatterns. Results show that significant adsorption is achieved on the target protein, with unspecific adsorptions being heavily suppressed on control proteins. In conclusion, we have successfully manufactured microcapsules that specifically and exclusively bind to their complementary target area. This paves the way for future in vivo experiments using microcapsules as targeted drug carriers.
Institute of Bioengineering, Queen Mary University of London , Mile End Road, London E1 4NS, United Kingdom.
Devendra I
DI
Deo
Julien E
JE
Gautrot
Gleb B
GB
Sukhorukov
Wen
W
Wang
D000327:Adsorption; D055910:Antibodies, Immobilized / Q000737:chemistry; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry; D002214:Capsules; D024141:Collagen Type IV / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis*; D005353:Fibronectins / Q000737:chemistry; D006801:Humans; D011092:Polyethylene Glycols / Q000138:chemical synthesis*; D011485:Protein Binding; D013499:Surface Properties
D055910:Antibodies, Immobilized; D058846:Antibodies, Monoclonal, Murine-Derived; D002214:Capsules; D024141:Collagen Type IV; D004337:Drug Carriers; D005353:Fibronectins; D011092:Polyethylene Glycols