Analysis of Th1, Th17 and regulatory T cells in tuberculosis case contacts.
We have hypothesized that individuals infected with Mycobacteriumtuberculosis that exhibit different patterns of immune reactivity in serial interferon (IFN)-γ release assays (IGRA's) correspond to different status within the immune spectrum of latent tuberculosis (TB). Accordingly, we analyzed the possible association between the consistent results (negative or positive) in an IGRA test and relevant immune parameters, mainly the levels of Th1 and Th17 lymphocytes and T regulatory (Treg) cells in the peripheral blood of TB case contacts. We found that individuals with a persistently positive IGRA showed increased levels of Th1 and Th17 lymphocytes upon in vitro stimulation with MTB antigens. In addition, a significant increase in the proportion of CD4+CTLA-4+ and CD4+Foxp3+ cells was detected in assays with blood samples from these individuals. Our data support that different immune phenotypes can be identified into the spectrum of latent TB, by combining different parameters of immune reactivity against MTB.
Unidad de Investigación Médica-Zacatecas, Instituto Mexicano del Seguro Social, IMSS, Mexico.
R E
RE
García Jacobo
Unidad de Investigación Médica-Zacatecas, Instituto Mexicano del Seguro Social, IMSS, Mexico.
C J
CJ
Serrano
Unidad de Investigación Médica-Zacatecas, Instituto Mexicano del Seguro Social, IMSS, Mexico.
J A
JA
Enciso Moreno
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Unidad Noreste., Monterrey, Nuevo León, Mexico.
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O
Gaspar Ramírez
Unidad de Investigación Médica-Zacatecas, Instituto Mexicano del Seguro Social, IMSS, Mexico.
J L
JL
Trujillo Ochoa
Departamento de Inmunología y Biología Celular y Molecular, Facultad de Ciencias Químicas, UASLP, San Luis Potosí, SLP, Mexico.
E E
EE
Uresti Rivera
Departamento de Inmunología y Biología Celular y Molecular, Facultad de Ciencias Químicas, UASLP, San Luis Potosí, SLP, Mexico.
D P
DP
Portales Pérez
Departamento de Inmunología, Facultad de Medicina, UASLP, San Luis Potosí, SLP, Mexico.
R
R
González-Amaro
Unidad de Investigación Médica-Zacatecas, Instituto Mexicano del Seguro Social, IMSS, Mexico. Electronic address: mariana.haydee.gh@gmail.com.
M H
MH
García Hernández
D000328:Adult; D015704:CD4 Antigens / Q000097:blood; D060908:CTLA-4 Antigen / Q000097:blood; D005260:Female; D051858:Forkhead Transcription Factors / Q000097:blood; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D059425:Interferon-gamma Release Tests; D055985:Latent Tuberculosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*
D015704:CD4 Antigens; D060908:CTLA-4 Antigen; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D007371:Interferon-gamma
Immune response; Mycobacteria; T lymphocytes; TB spectrum; Tuberculosis case contacts