A novel application of pulsed electric field (PEF) processing for improving glutathione (GSH) antioxidant activity.
Glutathione (GSH) was treated by pulsed electric field (PEF) processing to investigate its effect on antioxidant activity. The antioxidant activity of GSH was evaluated using 2,2-diphenyl-1-picrylhydrazy (DPPH) radical inhibition. A Box-Behnken design (BBD) with three independent variables, which were concentration, electric field intensity and pulse frequency was used to establish the regression equation of second-order response surface. Optimal conditions were as follows: GSH concentration 8.86mg/mL, electric field intensity 9.74kV/cm and pulse frequency 2549.08Hz. The DPPH radical inhibition increased from 81.83% to 97.40%. Near-infrared spectroscopy (NIR) and mid-infrared spectroscopy (MIR) were used to analyse the change of structure and functional groups of GSH.
Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, PR China.
Jia
J
Wang
Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, PR China.
Ke
K
Wang
Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, PR China.
Ying
Y
Wang
Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, PR China. Electronic address: linsongyi730@163.com.
Songyi
S
Lin
Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, PR China.
Ping
P
Zhao
Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, SC 29634, USA.
Gregory
G
Jones
D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D004560:Electricity; D005511:Food Handling / Q000379:methods*; D005978:Glutathione / Q000494:pharmacology*; D010851:Picrates / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared
D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl; D005978:Glutathione
Analysis of structure and functional groups; Glutathione (GSH); Improvement antioxidant activity; Pulsed electric field (PEF); Response surface methodology (RSM)