Methoxy-poly(ethylene glycol) modified poly(L-lactide) enhanced cell affinity of human bone marrow stromal cells by the upregulation of 1-cadherin and delta-2-catenin.
Poly(L-lactide) (PLLA), a versatile biodegradable polymer, is one of the most commonly-used materials for tissue engineering applications. To improve cell affinity for PLLA, poly(ethylene glycol) (PEG) was used to develop diblock copolymers. Human bone marrow stromal cells (hBMSCs) were cultured on MPEG-b-PLLA copolymer films to determine the effects of modification on the attachment and proliferation of hBMSC. The mRNA expression of 84 human extracellular matrix (ECM) and adhesion molecules was analyzed using RT-qPCR to understand the underlying mechanisms. It was found that MPEG-b-PLLA copolymer films significantly improved cell adhesion, extension, and proliferation. This was found to be related to the significant upregulation of two adhesion genes, CDH1 and CTNND2, which encode 1-cadherin and delta-2-catenin, respectively, two key components for the cadherin-catenin complex. In summary, MPEG-b-PLLA copolymer surfaces improved initial cell adhesion by stimulation of adhesion molecule gene expression.
Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 Ling Yuan Road West, Guangzhou 510055, China ; Guangdong Provincial Key Laboratory of Stomatology, 56 Ling Yuan Road West, Guangzhou 510055, China ; Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4059, Australia.
Xueli
X
Mao
Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4059, Australia ; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4059, Australia.
Zetao
Z
Chen
Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 Ling Yuan Road West, Guangzhou 510055, China ; Guangdong Provincial Key Laboratory of Stomatology, 56 Ling Yuan Road West, Guangzhou 510055, China ; Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4059, Australia.
Junqi
J
Ling
Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 Ling Yuan Road West, Guangzhou 510055, China ; Guangdong Provincial Key Laboratory of Stomatology, 56 Ling Yuan Road West, Guangzhou 510055, China.
Jingjing
J
Quan
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Corner College and Cooper Roads, Brisbane, QLD 4072, Australia.
Hui
H
Peng
Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4059, Australia ; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4059, Australia.
Yin
Y
Xiao
D015820:Cadherins / Q000378:metabolism*; D051177:Catenins / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008875:Middle Aged; D011091:Polyesters / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects*; D000095002:Delta Catenin
D015820:Cadherins; D051177:Catenins; D011091:Polyesters; C492425:methoxy poly(ethylene glycol)-poly(lactide); D011092:Polyethylene Glycols; D000095002:Delta Catenin; C000722348:CTNND2 protein, human