Human aquaporin-11 is a water and glycerol channel and localizes in the vicinity of lipid droplets in human adipocytes.
OBJECTIVE
For a long time Aquaporin-7 has been the only aquaporin associated with the adipose tissue, and its dysregulation has been linked to the underlying mechanisms of obesity. However, the presence of alternative glycerol channels within the adipose tissue has been postulated, which has prompted us to the search of alternate glycerol transport routes in adipocytes. In view of this, it is hypothesized that Aquaporin-11 (AQP11) would have a role in adipocyte cell biology.


METHODS
The expression, the localization and the function of human AQP11 (hAQP11) in cultured differentiated adipocytes were investigated.


RESULTS
Gene expression analysis revealed the presence of AQP11 in both subcutaneous and visceral human mature adipocytes. It is found that hAQP11 is primarily located intracellularly in human adipocytes and partially colocalizes with perilipin, pointing towards AQP11 preferential location in the vicinity of lipid droplets. Overexpression of hAQP11 in 3T3-L1 adipocytes enabled to validate its function as a water channel and reveal its glycerol permeation activity.


CONCLUSIONS
hAQP11 permeates both water and glycerol, localizing in the vicinity of lipid droplets in human adipocytes.
Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Lisboa, Portugal; Department Bioquimica e Biologia Humana, Faculdade de Farmacia, Universidade de Lisboa, Portugal.
Ana
A
Madeira
Sonia
S
Fernández-Veledo
Marta
M
Camps
Antonio
A
Zorzano
Teresa F
TF
Moura
Victoria
V
Ceperuelo-Mallafré
Joan
J
Vendrell
Graça
G
Soveral
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D020346:Aquaporins / Q000378:metabolism / Q000502:physiology*; D001692:Biological Transport; D002478:Cells, Cultured; D005990:Glycerol / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D066292:Lipid Droplets / Q000378:metabolism*; D051379:Mice; D014018:Tissue Distribution; D014867:Water / Q000378:metabolism*
C511251:AQP11 protein, human; D020346:Aquaporins; D014867:Water; D005990:Glycerol