Association of endothelial nitric oxide synthase gene polymorphisms (894G/T, -786T/C, G10T) and clinical findings in patients with migraine.
Migraine is a common neurological disorder characterized by recurrent attacks, unilateral head pain, and related symptoms. The aim of this study was to investigate three endothelial nitric oxide synthase (eNOS) polymorphisms in 176 patients with migraine and 123 healthy individuals. Clinical and biochemical parameters were investigated. Genetic analysis was performed using the polymerase chain reaction-restriction fragment length polymorphism method. The differences between migraine cases and the control group were significant for two polymorphisms (-786T/C and 894G/T) (p = 0.000). Homocysteine and body mass index (BMI) were significantly higher in the migraine group than in the control group (p = 0.001 and p = 0.000). The relation between -786T/C genotype and BMI and allodynia was significant. TC heterozygotes and CC homozygotes were significantly higher in the migraine group than in the control group (OR 2.843 and 95 % CI 1.681-4.808 and OR 3.729 and 95 % CI 1.784-7.792, respectively). The 894G/T genotype was correlated with BMI, pain intensity, age at the onset of migraine, nausea, tension, compression, and allodynia. For this polymorphism, GT heterozygotes and TT homozygotes were significantly higher in the migraine group than in the control group (OR 3.027 and 95 % CI 1.830-5.008 and OR 3.221 and 95 % CI 1.223-8.484, respectively). The G10T genotype was correlated with attack duration and age at the onset of migraine (p = 0.008 and p = 0.040). eNOS polymorphisms may be useful markers for assessing migraine risk and clinical diagnosis.
Department of Medical Genetics, Medical School, Duzce University, Duzce, 81620, Turkey, eroz38r@hotmail.com.
Recep
R
Eröz
Anzel
A
Bahadir
Suber
S
Dikici
Sener
S
Tasdemir
D000293:Adolescent; D000328:Adult; D017668:Age of Onset; D000483:Alleles; D015992:Body Mass Index; D016022:Case-Control Studies; D015897:Comorbidity; D005091:Exons / Q000235:genetics; D005260:Female; D005838:Genotype; D006710:Homocysteine / Q000097:blood; D006801:Humans; D012001:Hyperacusis / Q000209:etiology / Q000235:genetics; D006930:Hyperalgesia / Q000453:epidemiology / Q000235:genetics; D007438:Introns / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020325:Migraine with Aura / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D020326:Migraine without Aura / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D009325:Nausea / Q000209:etiology / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D050177:Overweight / Q000453:epidemiology / Q000235:genetics; D020795:Photophobia / Q000209:etiology / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D055815:Young Adult
D006710:Homocysteine; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III