PrecisePrimer: an easy-to-use web server for designing PCR primers for DNA library cloning and DNA shuffling.
PrecisePrimer is a web-based primer design software made to assist experimentalists in any repetitive primer design task such as preparing, cloning and shuffling DNA libraries. Unlike other popular primer design tools, it is conceived to generate primer libraries with popular PCR polymerase buffers proposed as pre-set options. PrecisePrimer is also meant to design primers in batches, such as for DNA libraries creation of DNA shuffling experiments and to have the simplest interface possible. It integrates the most up-to-date melting temperature algorithms validated with experimental data, and cross validated with other computational tools. We generated a library of primers for the extraction and cloning of 61 genes from yeast DNA genomic extract using default parameters. All primer pairs efficiently amplified their target without any optimization of the PCR conditions.
Institute of System and Synthetic Biology, Université d'Évry val d'Éssonnes, Bt. Geneavenir 6 Genopole Campus 1, 5 rue Henry Desbruères 91000, Évry, France.
Cyrille
C
Pauthenier
Institute of System and Synthetic Biology, Université d'Évry val d'Éssonnes, Bt. Geneavenir 6 Genopole Campus 1, 5 rue Henry Desbruères 91000, Évry, France jean-loup.faulon@issb.genopole.fr.
Jean-Loup
JL
Faulon
D000465:Algorithms; D003001:Cloning, Molecular; D017931:DNA Primers; D032621:DNA Shuffling; D015723:Gene Library; D005800:Genes, Fungal; D020407:Internet; D016133:Polymerase Chain Reaction / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012984:Software
D017931:DNA Primers