iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo.
Comprehensive and quantitative proteomic information on citrus floral bud is significant for understanding male sterility of the cybrid pummelo (G1+HBP) with nuclear genome of HBP and foreign mitochondrial genome of G1. Scanning electron microscopy and transmission electron microscopy analyses of the anthers showed that the development of pollen wall in G1+HBP was severely defective with a lack of exine and sporopollenin formation. Proteomic analysis was used to identify the differentially expressed proteins between male sterile G1+HBP and fertile type (HBP) with the aim to clarify their potential roles in anther development and male sterility. On the basis of iTRAQ quantitative proteomics, we identified 2235 high-confidence protein groups, 666 of which showed differentially expressed profiles in one or more stages. Proteins up- or down-regulated in G1+HBP were mainly involved in carbohydrate and energy metabolism (e.g., pyruvate dehydrogenase, isocitrate dehydrogenase, ATP synthase, and malate dehydrogenase), nucleotide binding (RNA-binding proteins), protein synthesis and degradation (e.g., ribosome proteins and proteasome subunits). Additionally, the proteins located in mitochondria also showed changed expression patterns. These findings provide a valuable inventory of proteins involved in floral bud development and contribute to elucidate the mechanism of cytoplasmic male sterility in the cybrid pummelo.
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University , Wuhan 430070, China.
Bei-Bei
BB
Zheng
Yan-Ni
YN
Fang
Zhi-Yong
ZY
Pan
Li
L
Sun
Xiu-Xin
XX
Deng
Jude W
JW
Grosser
Wen-Wu
WW
Guo
D050260:Carbohydrate Metabolism; D002957:Citrus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D035264:Flowers / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D012098:Reproduction; D013194:Staining and Labeling; D059467:Transcriptome
D024101:Mitochondrial Proteins; D010940:Plant Proteins; D020543:Proteome