Polymerase synthesis of photocaged DNA resistant against cleavage by restriction endonucleases.
5-[(2-Nitrobenzyl)oxymethyl]-2'-deoxyuridine 5'-O-triphosphate was used for polymerase (primer extension or PCR) synthesis of photocaged DNA that is resistant to the cleavage by restriction endonucleases. Photodeprotection of the caged DNA released 5-hydroxymethyluracil-modified nucleic acids, which were fully recognized and cleaved by restriction enzymes.
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, 16610 Prague 6 (Czech Republic) http://www.uochb.cas.cz/hocekgroup.
Zuzana
Z
Vaníková
Michal
M
Hocek
D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D010432:Pentoxyl / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D014466:Ultraviolet Rays
C008055:5-hydroxymethyluracil; D010432:Pentoxyl; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes
DNA; DNA polymerase; nucleotides; photocaging; protective groups