Are basophil activation and sulphidoleukotriene determination useful tests for monitoring patients with peach allergy receiving sublingual immunotherapy with a Pru p 3-enriched peach extract?
BACKGROUND
Treatment of food allergy essentially consists of food avoidance, but immunotherapy with food is emerging as a new therapeutic option.


OBJECTIVE
To evaluate clinical improvement and immunological changes in patients with peach allergy following sublingual immunotherapy (SLIT) with a Prup3 quantified peach extract.


METHODS
A randomized, double-blind, placebo-controlled clinical trial with peach SLIT was conducted. We assessed clinical efficacy after 6 months of treatment by means of double-blind, placebo-controlled oral challenges with peach and also evaluated immunological changes (basophil activation test [BAT] and determination of sulphidoleukotriene production) following stimulation with peach peel and pulp, rPrup3, rMald 1, and rMal d 4 stimulation. We also measured specific IgE and IgG4 to Pru p3.


RESULTS
After 6 months of SLIT (T6), the active group showed a 3-fold improvement in tolerance to Prup3 and a significant increase in IgE to rPrup3 and in sLT production following stimulation with peach peel and rPrup3. There was also a significant increase in BAT results after stimulation with rPrup3 at 1 month of SLIT (T1). Statistically significant between-group differences were only observed for BAT with peach peel and pulp at T1 and T6 and for BAT with rPru p3 at T6. No changes were observed in BAT with rMal d 1 or rMal d 4 or in IgG4 levels to nPrup3.


CONCLUSIONS
SLIT with a Pru p 3 quantified peach extract is clinically effective and leads to an increase in basophil activation and sulphidoleukotriene production following stimulation with rPru p3 and peach peel in the first months of treatment.
S
S
Garrido-Fernández
B E
BE
García
M L
ML
Sanz
S
S
Echechipía
M T
MT
Lizaso
A I
AI
Tabar
D000328:Adult; D052179:Antigens, Plant / Q000276:immunology*; D001491:Basophils / Q000276:immunology*; D004311:Double-Blind Method; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology / Q000628:therapy*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D015289:Leukotrienes / Q000096:biosynthesis*; D008297:Male; D010936:Plant Extracts / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D027861:Prunus / Q000276:immunology*; D063729:Sublingual Immunotherapy
D052179:Antigens, Plant; D007074:Immunoglobulin G; D015289:Leukotrienes; D010936:Plant Extracts; D010940:Plant Proteins; C463181:Pru p 3 allergen; D007073:Immunoglobulin E