Yeast transformation by the LiAc/SS carrier DNA/PEG method.
Transformation is essential to many molecular and genetic investigations in the yeast Saccharomyces cerevisiae. Yeast transformation protocols utilizing the LiAc/ssDNA/PEG method are presented. Protocols for various applications are listed including a method for transformation in 96-well microtiter plate format and another for the production of frozen competent yeast cells that can be used at a moment's notice.
Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Avenue, Winnipeg, MB, Canada, R3E 0W3, gietz@cc.umanitoba.ca.
R Daniel
RD
Gietz
D000085:Acetates / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D006358:Hot Temperature; D008967:Molecular Biology / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D013388:Succinimides / Q000737:chemistry; D014170:Transformation, Genetic
D000085:Acetates; D013388:Succinimides; D011092:Polyethylene Glycols; C050761:polyethylene glycol bis(succinimidyl succinate); D004247:DNA; C488804:lithium acetate