Preparation of immobilized glucose oxidase and its application in improving breadmaking quality of commercial wheat flour.
Preparation of immobilized glucose oxidase (GO) on chitosan (CS)-sodium tripolyphosphate (TPP) and its application in improving breadmaking quality of commercial wheat flour were investigated. The optimum conditions for GO immobilization were: viscosity of CS: 700cP, ratio of CS to TPP (w/w): 5 to 1, and GO concentration 100U/mL. The obtained CSTPP-GO was 5μm-diameter particle with a pseudo-spherical shape. By addition of CSTPP-GO (400U/kg flour) and fungal α-amylase (62.5U/kg flour), bread springiness slightly increased from 0.923 to 0.940, specific volume of crumb increased by 13.48% and hardness decreased by 19.22%, compared to addition of KBrO3 (60mg/kg flour). It could be concluded that CSTPP-GO combined with fungal α-amylase had potential application in improving breadmaking quality of commercial wheat flour.
State Key Laboratory of Food Science and Technology, Jiangnan University, 214122 Wuxi, China; School of Food Science and Technology, Jiangnan University, 214122 Wuxi, China.
Lele
L
Tang
State Key Laboratory of Food Science and Technology, Jiangnan University, 214122 Wuxi, China; School of Food Science and Technology, Jiangnan University, 214122 Wuxi, China. Electronic address: yrj@jiangnan.edu.cn.
Ruijin
R
Yang
School of Food Science and Technology, Jiangnan University, 214122 Wuxi, China.
Xiao
X
Hua
State Key Laboratory of Food Science and Technology, Jiangnan University, 214122 Wuxi, China.
Chaohua
C
Yu
School of Food Science and Technology, Jiangnan University, 214122 Wuxi, China.
Wenbin
W
Zhang
State Key Laboratory of Food Science and Technology, Jiangnan University, 214122 Wuxi, China.
Wei
W
Zhao
D001939:Bread / Q000032:analysis*; D005433:Flour / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry*
D005949:Glucose Oxidase
Breadmaking quality; Chitosan; Commercial wheat flour; Glucose oxidase; Immobilization; Sodium tripolyphosphate