Helicobacter pylori TlyA agglutinates liposomes and induces fusion and permeabilization of the liposome membranes.
Helicobacter pylori TlyA is a pore-forming hemolysin with potent cytotoxic activity. To explore the potential membrane-damaging activity of H. pylori TlyA, we have studied its interaction with the synthetic liposome vesicles. In our study, H. pylori TlyA shows a prominent ability to associate with the liposome vesicles without displaying an obligatory requirement for any protein receptor on the liposome membranes. Interaction of TlyA triggers agglutination of the liposome vesicles. Such agglutinating activity of TlyA could also be observed with erythrocytes before the induction of its pore-forming hemolytic activity. In addition to its agglutinating activity against liposomes, TlyA also induces fusion and disruption of the liposome membranes. Altogether, our study highlights novel membrane-damaging properties of H. pylori TlyA that have not been documented previously with any other TlyA family protein.
Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali , Sector 81, SAS Nagar, Manauli 140306, Punjab, India.
Kusum
K
Lata
Kausik
K
Chattopadhyay
D000371:Agglutination / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry / Q000502:physiology*; D002459:Cell Fusion; D002463:Cell Membrane Permeability / Q000502:physiology; D018360:Crystallography, X-Ray; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D006460:Hemolysin Proteins / Q000737:chemistry; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism*; D008561:Membrane Fusion / Q000502:physiology*; D037521:Virulence Factors / Q000737:chemistry / Q000502:physiology*
D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D008051:Lipid Bilayers; D008081:Liposomes; C588092:TlyA protein, Helicobacter pylori; D037521:Virulence Factors