Bacteriological and Molecular Detection of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus in Equines of Northern India.
Present study was undertaken to study the prevalence of β-haemolytic streptococci in equine of northern temperate region of Jammu and Kashmir, India. One hundred and forty one samples were collected in duplicate from nasopharyngeal tract of diseased (53) and apparently healthy equine (88) for isolation and direct PCR. A total of 77 isolates of streptococci were recovered from 141 samples with an overall prevalence rate of 54.60%. Out of these 77 isolates, 52 were from diseased and 25 from apparently healthy animals. Of the 77 isolates, 4 were identified as Streptococcus equi subsp. equi, 56 as S. equi subsp. zooepidemicus and 17 as S. dysgalactiae subsp. equisimilis. Thus the overall prevalence of S. equi subsp. equi, S. equi subsp. zooepidemicus and S. dysgalactiae subsp. equisimilis was 2.83, 39.71 and 12.05% respectively. The sensitivity of the PCR for the detection of S. equi species was found higher when attempted from direct swab samples.
Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu 181102, India.
Irfan Ahmad
IA
Mir
Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
Bablu
B
Kumar
Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu 181102, India.
Anil
A
Taku
Division of Veterinary Microbiology and Biotechnology, Rajasthan University of Veterinary and Animal Sciences, Bikaner 334001, India.
Farah
F
Faridi
Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu 181102, India.
Mohd Altaf
MA
Bhat
Division of Veterinary Pharmacology & Toxicology, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu 181102, India.
Naseer Ahmad
NA
Baba
Division of Veterinary Public Health & Epidemiology, Sher-e-Kashmir University of Agricultural Sciences & Technology, Srinagar 190001, India.
Tahir
T
Maqbool
S. dysgalactiae subsp. equisimilis; S. equi subsp. zooepidemicus; Streptococcus equi subsp. equi; equine