Structure and genetics of the O-antigen of Enterobacter cloacae C6285 containing di-N-acetyllegionaminic acid.
On mild acid degradation of the lipopolysaccharide of Enterobacter cloacae C6285, the O-polysaccharide was cleaved at residues of 5,7-diacetamido-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic acid (di-N-acetyllegionaminic acid, Leg5Ac7Ac) in the main chain. The resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with (1)H and (13)C NMR spectroscopy, and the following structure of the tetrasaccharide repeating unit of the linear O-polysaccharide was established: →4)-α-d-Galp-(1→4)-α-Legp5Ac7Ac-(2→3)-β-d-Galp-(1→3)-β-d-GalpNAc-(1→ The O-antigen gene cluster of E. cloacae C6285 was sequenced, the gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
Andrei V
AV
Filatov
TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, PR China; Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, TEDA, Tianjin 300457, PR China.
Min
M
Wang
Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, PR China.
Wenjing
W
Wang
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia. Electronic address: perepel@ioc.ac.ru.
Andrei V
AV
Perepelov
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
Alexander S
AS
Shashkov
TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, PR China; Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, TEDA, Tianjin 300457, PR China.
Lei
L
Wang
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
Yuriy A
YA
Knirel
D002240:Carbohydrate Sequence; D016972:Enterobacter cloacae / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*; D012794:Sialic Acids / Q000737:chemistry*
C110570:5,7-diacetamido-8-O-acetyl-3,5,7,9-tetradeoxy-glycero-talo-nonulosonic acid; D019081:O Antigens; D012794:Sialic Acids
Bacterial polysaccharide structure; Enterobacter cloacae; Legionaminic acid; O-Antigen; O-Antigen gene cluster