CaMKK-CaMK1a, a new post-traumatic signalling pathway induced in mouse somatosensory neurons.
Neurons innervating peripheral tissues display complex responses to peripheral nerve injury. These include the activation and suppression of a variety of signalling pathways that together influence regenerative growth and result in more or less successful functional recovery. However, these responses can be offset by pathological consequences including neuropathic pain. Calcium signalling plays a major role in the different steps occurring after nerve damage. As part of our studies to unravel the roles of injury-induced molecular changes in dorsal root ganglia (DRG) neurons during their regeneration, we show that the calcium calmodulin kinase CaMK1a is markedly induced in mouse DRG neurons in several models of mechanical peripheral nerve injury, but not by inflammation. Intrathecal injection of NRTN or GDNF significantly prevents the post-traumatic induction of CaMK1a suggesting that interruption of target derived factors might be a starter signal in this de novo induction. Inhibition of CaMK signalling in injured DRG neurons by pharmacological means or treatment with CaMK1a siRNA resulted in decreased velocity of neurite growth in vitro. Altogether, the results suggest that CaMK1a induction is part of the intrinsic regenerative response of DRG neurons to peripheral nerve injury, and is thus a potential target for therapeutic intervention to improve peripheral nerve regeneration.
Institute for Neurosciences of Montpellier, I.N.S.E.R.M. U1051, Montpellier, France.
Lucie
L
Elzière
Institute for Neurosciences of Montpellier, I.N.S.E.R.M. U1051, Montpellier, France.
Chamroeun
C
Sar
Institute for Neurosciences of Montpellier, I.N.S.E.R.M. U1051, Montpellier, France.
Stéphanie
S
Ventéo
Molecular Neurobiology Laboratory, The Salk Institute, La Jolla, California, United States of America.
Steeve
S
Bourane
Institute for Neurosciences of Montpellier, I.N.S.E.R.M. U1051, Montpellier, France.
Sylvie
S
Puech
Institute for Neurosciences of Montpellier, I.N.S.E.R.M. U1051, Montpellier, France.
Corinne
C
Sonrier
Institute for Neurosciences of Montpellier, I.N.S.E.R.M. U1051, Montpellier, France.
Hassan
H
Boukhadaoui
Institute for Neurosciences of Montpellier, I.N.S.E.R.M. U1051, Montpellier, France; Department BioMV, University of Montpellier II, Montpellier, France.
Agnès
A
Fichard
Institute for Neurosciences of Montpellier, I.N.S.E.R.M. U1051, Montpellier, France.
Alexandre
A
Pattyn
Institute for Neurosciences of Montpellier, I.N.S.E.R.M. U1051, Montpellier, France; Department BioMV, University of Montpellier II, Montpellier, France.
Jean
J
Valmier
Institute for Neurosciences of Montpellier, I.N.S.E.R.M. U1051, Montpellier, France.
Patrick
P
Carroll
Institute for Neurosciences of Montpellier, I.N.S.E.R.M. U1051, Montpellier, France; Department BioMV, University of Montpellier II, Montpellier, France.
Ilana
I
Méchaly
D000818:Animals; D019771:Axotomy; D020013:Calcium Signaling / Q000235:genetics / Q000502:physiology*; D054729:Calcium-Calmodulin-Dependent Protein Kinase Type 1 / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology* / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D009416:Nerve Regeneration / Q000502:physiology*; D016501:Neurites / Q000502:physiology; D009474:Neurons / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012584:Sciatic Nerve / Q000601:surgery
D054729:Calcium-Calmodulin-Dependent Protein Kinase Type 1