Development and application of quartz crystal microbalance sensor based on novel molecularly imprinted sol-gel polymer for rapid detection of histamine in foods.
To rapidly detect histamine (HA) in foods, a novel material for HA-specific recognition was synthesized by a sol-gel process and coated on a quartz crystal microbalance (QCM) sensor. The Scatchard model was used to evaluate the adsorption performance of the material; high affinity for HA was demonstrated. Based on QCM frequency change, the sensor exhibited linear behavior for HA concentrations of 0.11 × 10(-2) to 4.45 × 10(-2) mg L(-1), a detection limit of 7.49 × 10(-4) mg kg(-1) (S/N = 3), high selectivity for HA (selectivity coefficient >4) compared with structural analogues, good reproducibility, and long-term stability. The sensor was used to determine the concentration of HA in spiked fish products; the recovery values were satisfactory (93.2-100.4%) and compared well with those obtained by high-performance liquid chromatography (correlation coefficient, r(2) = 0.9965).
Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology , Tianjin 300457, China.
Jie
J
Dai
Yan
Y
Zhang
Mingfei
M
Pan
Lingjie
L
Kong
Shuo
S
Wang
D000327:Adsorption; D000818:Animals; D005396:Fish Products / Q000032:analysis*; D005399:Fishes; D005782:Gels / Q000737:chemistry; D006632:Histamine / Q000032:analysis*; D057230:Limit of Detection; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011791:Quartz / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques / Q000295:instrumentation / Q000379:methods*
D005782:Gels; D011108:Polymers; D011791:Quartz; D006632:Histamine