An exact solution for R2,eff in CPMG experiments in the case of two site chemical exchange.
The Carr-Purcell-Meiboom-Gill (CPMG) experiment is widely used to quantitatively analyse the effects of chemical exchange on NMR spectra. In a CPMG experiment, the effective transverse relaxation rate, R2,eff, is typically measured as a function of the pulse frequency, νCPMG. Here, an exact expression for how R2,eff varies with νCPMG is derived for the commonly encountered scenario of two-site chemical exchange of in-phase magnetisation. This result, summarised in Appendix A, generalises a frequently used equation derived by Carver and Richards, published in 1972. The expression enables more rapid analysis of CPMG data by both speeding up calculation of R2,eff over numerical methods by a factor of ca. 130, and yields exact derivatives for use in data analysis. Moreover, the derivation provides insight into the physical principles behind the experiment.
Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford OX1 3QZ, UK. Electronic address: andrew.baldwin@chem.ox.ac.uk.
Andrew J
AJ
Baldwin
D000465:Algorithms; D003198:Computer Simulation; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
CPMG experiments; Carver Richards equation; Chemical exchange; Two-site exchange