A movable microelectrode array for chronic basal ganglia single-unit electrocorticogram co-recording in freely behaving rats.
The basal ganglia-cortical circuits are important for information process to brain function. However, chronic recording of single-unit activities in the basal ganglia nucleus has not yet been well established. We present a movable bundled microwire array for chronic subthalamic nucleus (STN) single-unit electrocorticogram co-recording. The electrode assembly contains a screw-advanced microdrive and a microwire array. The array consists of a steel guide tube, five recording wires and one referenced wire which form the shape of a guiding hand, and one screw electrode for cortico-recording. The electrode can acquire stable cortex oscillation-driven STN firing units in rats under different behaving conditions for 8 weeks. We achieved satisfying signal-to-noise ratio, portions of cells retaining viability, and spike waveform similarities across the recording sections. Using this method, we investigated neural correlations of the basal ganglia-cortical circuits in different behaving conditions. This method will become a powerful tool for multi-region recording to study normal statements or movement disorders.
Department of Neurosurgery, Key Clinic Specialty Discipline of Fujian, First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian, China.
Xiaobin
X
Zheng
Jia
J
Zeng
Ting
T
Chen
Yuanxiang
Y
Lin
Lianghong
L
Yu
Ying
Y
Li
Zhangya
Z
Lin
Xiyue
X
Wu
Fuyong
F
Chen
Dezhi
D
Kang
Shizhong
S
Zhang
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001479:Basal Ganglia / Q000166:cytology*; D004569:Electroencephalography / Q000295:instrumentation / Q000379:methods*; D005071:Evoked Potentials / Q000502:physiology*; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014851:Wakefulness / Q000502:physiology*