Establishment and characterization of a piscean PCF cell line for toxicity and gene expression studies as in vitro model.
A new piscean fibroblastic cell line termed as PCF derived from the caudal fin tissue of dark mahseer, Puntius (Tor) chelynoides was established and characterized in the present study which was found to be suitable for toxicity and gene expression studies as in vitro model. The cell line grew well in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum (FBS). The cells were able to grow at a temperature ranging from 20 to 28 °C with an optimal growth at 24 °C and the cell line have been expanded in culture for more than 70 passages. Authentication of the cell line was carried out using mitochondrial DNA markers (Cytochrome Oxidase subunit I and 16S ribosomal RNA). Presence of vimentin in the cells confirmed the fibroblastic origin of cell line. Significant cytopathic effects were observed upon exposure of PCF cell line to bacterial extracellular products and the study also validated the suitability of cell line in transgenic applications as well as in genotoxicity assessment as an in vitro model.
National Bureau of Fish Genetic Resources (NBFGR), Canal Ring Road, PO Dilkusha, Lucknow 226002, India. Electronic address: mukugoswami@gmail.com.
M
M
Goswami
National Bureau of Fish Genetic Resources (NBFGR), Canal Ring Road, PO Dilkusha, Lucknow 226002, India.
B S
BS
Sharma
National Bureau of Fish Genetic Resources (NBFGR), Canal Ring Road, PO Dilkusha, Lucknow 226002, India.
Kamalendra
K
Yadav
HNB Garhwal University, PO Box 70, Srinagar/Garhwal, Uttarakhand, India.
S N
SN
Bahuguna
Central Institute of Fisheries Education (CIFE), Versova, Andheri (W), Mumbai 400061, India.
W S
WS
Lakra
D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D015925:Cryopreservation / Q000379:methods; D005347:Fibroblasts / Q000166:cytology*; D005399:Fishes; D015870:Gene Expression / Q000502:physiology*; D023421:Models, Animal; D018675:Toxicity Tests / Q000379:methods
Cell line; Characterization; Genotoxicity; PCF; Transfection