Application of ultrasound technology in the study of ischemic postconditioning to protect testes from ischemia-reperfusion injury.
This study was designed to investigate the application of ultrasound technology in the study of ischemic postconditioning to protect testes from ischemia-reperfusion injury. Seventy-two big white rabbits were divided into mild ischemic groups (Group A: A0, A1, A2, A3), moderate ischemic groups (Group B: B0, B1, B2, B3) under ultrasound monitor, and control group (N = 8). Groups A0 and B0 received direct perfusion, while the other groups received a different short time filling/stopped filling treatment (15 s/15 s, 30 s/30 s, or 45 s/45 s) three times before complete perfusion. Each group received contrast-enhanced ultrasound before complete filling. At 3 days after perfusion, the testicular tissue was removed for biopsy. The parameters of testicular contrast in pre-reperfusion groups A and B differed significantly from those of their corresponding control groups (P < 0.05). The changes in testis-related pathological indicators in groups A1 and A2 were more significant than those of group A0 (P < 0.05), and changes in group B2 were more obvious than those of group B0 (P < 0.05). There were no statistically significant differences in the comparison of other indicators between the corresponding groups (P > 0.05). Ultrasound technology can help build different degree models of ischemic testes and predict the protective effect of post-ischemic treatment.
Department of Ultrasound, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian, China enshengxue@126.com.
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Xue
Department of Ultrasound, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian, China.
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Chen
Department of Ultrasound, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian, China.
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Wang
Department of Ultrasound, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian, China.
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Y
Zhang
Department of Ultrasound, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian, China.
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Chen
Department of Ultrasound, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian, China.
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Liang
D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D004195:Disease Models, Animal; D006801:Humans; D020287:In Situ Nick-End Labeling; D057775:Ischemic Postconditioning; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011817:Rabbits; D015427:Reperfusion Injury / Q000150:complications / Q000000981:diagnostic imaging*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013733:Testicular Diseases / Q000175:diagnosis / Q000000981:diagnostic imaging* / Q000209:etiology; D013737:Testis / Q000098:blood supply / Q000000981:diagnostic imaging* / Q000378:metabolism; D018615:Ultrasonography, Doppler, Color / Q000379:methods*
D019253:Proto-Oncogene Proteins c-bcl-2; D053148:Caspase 3