Type 1 fimbriae in commensal Escherichia coli derived from healthy humans.
Type 1 fimbriae are one of the most important factors of Escherichia coli adaptation to different niches in the host. Our study indicated that the genetic marker--fimH gene occurred commonly in commensal E. coli derived from healthy humans but expression of the type 1 fimbriae was not observed. Identification of fim structural subunit genes (fimA-fimH) and recombinase fimE and fimB genes showed that many of the strains were carrying an incomplete set of genes and the genes expression study revealed that in strains with complete set of fim genes, the fimC gene, encoding the chaperone protein, was not expressed.
Department of Biological Sciences, University of Zielona Góra, Poland.
Paweł
P
Pusz
Department of Biological Sciences, University of Zielona Góra, Poland.
Ewa
E
Bok
Department of Biological Sciences, University of Zielona Góra, Poland.
Justyna
J
Mazurek
Department of Biological Sciences, University of Zielona Góra, Poland.
Michał
M
Stosik
Department of Biological Sciences, University of Zielona Góra, Poland.
Katarzyna
K
Baldy-Chudzik
D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000235:genetics* / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000382:microbiology; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D009876:Operon; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D013559:Symbiosis
D018829:Adhesins, Bacterial