Is there a relationship between earthworm energy reserves and metal availability after exposure to field-contaminated soils?
Generic biomarkers are needed to assess environmental risks in metal polluted soils. We assessed the strength of the relationship between earthworm energy reserves and metal availability under conditions of cocktail of metals at low doses and large range of soil parameters. Aporrectodea caliginosa was exposed in laboratory to a panel of soils differing in Cd, Pb and Zn total and available (CaCl2 and EDTA-extractable) concentrations, and in soil texture, pH, CEC and organic-C. Glycogen, protein and lipid contents were recorded in exposed worms. Glycogen contents were not linked to the explaining variables considered. Variable selection identified CaCl2 extractable metals concentrations and soil texture as the main factors affecting protein and lipid contents. The results showed opposite effects of Pb and Zn, high inter-individual variability of biomarkers and weak relationships with easily extractable metals. Our results support the lack of genericity of energy reserves in earthworms exposed to field-contaminated soils.
INRA, UR 251 PESSAC, 78026 Versailles Cedex, France.
Léa
L
Beaumelle
INRA, UR 251 PESSAC, 78026 Versailles Cedex, France.
Isabelle
I
Lamy
INRA, UR 251 PESSAC, 78026 Versailles Cedex, France.
Nathalie
N
Cheviron
INRA, UR 251 PESSAC, 78026 Versailles Cedex, France. Electronic address: mickael.hedde@versailles.inra.fr.
Mickaël
M
Hedde
D000818:Animals; D002104:Cadmium / Q000737:chemistry / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D006003:Glycogen / Q000378:metabolism; D007854:Lead / Q000737:chemistry / Q000494:pharmacology; D019216:Metals, Heavy / Q000737:chemistry / Q000494:pharmacology*; D009835:Oligochaeta / Q000378:metabolism*; D012987:Soil; D012989:Soil Pollutants / Q000032:analysis / Q000494:pharmacology*; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology
D019216:Metals, Heavy; D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium; D007854:Lead; D006003:Glycogen; D015032:Zinc
Cocktail; Earthworm biomarkers; Low dose; Soil metal availability; Soil parameters