Expression of polysialylated neural cell adhesion molecules on adult stem cells after neuronal differentiation of inner ear spiral ganglion neurons.
During brain development, polysialylated (polySia) neural cell adhesion molecules (polySia-NCAMs) modulate cell-cell adhesive interactions involved in synaptogenesis, neural plasticity, myelination, and neural stem cell (NSC) proliferation and differentiation. Our findings show that polySia-NCAM is expressed on NSC isolated from adult guinea pig spiral ganglion (GPSG), and in neurons and Schwann cells after differentiation of the NSC with epidermal, glia, fibroblast growth factors (GFs) and neurotrophins. These differentiated cells were immunoreactive with mAb's to polySia, NCAM, β-III tubulin, nestin, S-100 and stained with BrdU. NSC could regenerate and be differentiated into neurons and Schwann cells. We conclude: (1) polySia is expressed on NSC isolated from adult GPSG and on neurons and Schwann cells differentiated from these NSC; (2) polySia is expressed on neurons primarily during the early stage of neuronal development and is expressed on Schwann cells at points of cell-cell contact; (3) polySia is a functional biomarker that modulates neuronal differentiation in inner ear stem cells. These new findings suggest that replacement of defective cells in the inner ear of hearing impaired patients using adult spiral ganglion neurons may offer potential hope to improve the quality of life for patients with auditory dysfunction and impaired hearing disorders.
Department of Otolaryngology Head & Neck Surgery, College of Medicine, Catholic University, Seoul, Republic of Korea.
Kyoung Ho
KH
Park
Department of Otolaryngology Head & Neck Surgery, College of Medicine, Catholic University, Seoul, Republic of Korea. Electronic address: swyeo@catholic.ac.kr.
Sang Won
SW
Yeo
Department of Biochemistry & Molecular Medicine, University of California, School of Medicine, Davis, CA 95616, USA; Xiamen University, School of Medicine, Xiamen City, PR China. Electronic address: fatroy@ucdavis.edu.
Frederic A
FA
Troy
D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D006168:Guinea Pigs; D006311:Hearing Disorders / Q000628:therapy; D006801:Humans; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry* / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism; D013136:Spiral Ganglion / Q000166:cytology* / Q000378:metabolism*
D019006:Neural Cell Adhesion Molecules; D012794:Sialic Acids
Neural cell adhesion molecules (NCAM); Polysialic acid; Polysialylated NCAM; Spiral ganglion stem cells