BEST: barcode enabled sequencing of tetrads.
Tetrad analysis is a valuable tool for yeast genetics, but the laborious manual nature of the process has hindered its application on large scales. Barcode Enabled Sequencing of Tetrads (BEST)1 replaces the manual processes of isolating, disrupting and spacing tetrads. BEST isolates tetrads by virtue of a sporulation-specific GFP fusion protein that permits fluorescence-activated cell sorting of tetrads directly onto agar plates, where the ascus is enzymatically digested and the spores are disrupted and randomly arrayed by glass bead plating. The haploid colonies are then assigned sister spore relationships, i.e. information about which spores originated from the same tetrad, using molecular barcodes read during genotyping. By removing the bottleneck of manual dissection, hundreds or even thousands of tetrads can be isolated in minutes. Here we present a detailed description of the experimental procedures required to perform BEST in the yeast Saccharomyces cerevisiae, starting with a heterozygous diploid strain through the isolation of colonies derived from the haploid meiotic progeny.
Pacific Northwest Diabetes Research Institute.
Adrian C
AC
Scott
Pacific Northwest Diabetes Research Institute.
Catherine L
CL
Ludlow
Pacific Northwest Diabetes Research Institute.
Gareth A
GA
Cromie
Pacific Northwest Diabetes Research Institute; aimee.dudley@gmail.com.
Aimée M
AM
Dudley
D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics; D004171:Diploidy; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D006238:Haploidy; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D008540:Meiosis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics*
D004271:DNA, Fungal