iTRAQ-based proteomic profiling of the marine medaka (Oryzias melastigma) gonad exposed to BDE-47.
A recent study demonstrated that 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) may have an adverse effect on the reproduction in marine medaka (Oryzias melastigma), but the molecular mechanisms remain largely unknown. In this study, we investigated the protein expression profiles of male and female gonads of O. melastigma exposed to dietary BDE-47 at two dosages (0.65 and 1.30 μg/g/day, respectively) for 21 days. Extracted proteins were labeled with iTRAQ and analyzed on a MALDI TOF/TOF analyzer, as results, 133 and 144 unique proteins were identified in testis and ovary, respective, and they exerted dose- and sex-dependent expression patterns. In testis, among the 42 differentially expressed proteins; down-regulation of histone variants and parvalbumins implicated BDE-47 may disrupt the spermatogenesis and induce sterility in fishes. In ovary, 38 proteins were differentially expressed; the elevation of vitellogenins and apolipoprotein A-I expression indicated BDE-47 acts as an estrogen-mimicking compound and led to reproductive impairment in O. melastigma.
State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong; Shenzhen Key Laboratory of Biochip Research, City University of Hong Kong, Shenzhen 518057, PR China.
C C
CC
Fong
Shenzhen Key Laboratory of Biochip Research, City University of Hong Kong, Shenzhen 518057, PR China; Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Y F
YF
Shi
State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong.
W K
WK
Yu
Shenzhen Key Laboratory of Biochip Research, City University of Hong Kong, Shenzhen 518057, PR China.
F
F
Wei
State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong.
J P
JP
van de Merwe
State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong.
Alice K Y
AK
Chan
State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong.
R
R
Ye
State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong.
Doris W T
DW
Au
School of Biological Science, The University of Hong Kong, Hong Kong.
Rudolf S S
RS
Wu
State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong; Shenzhen Key Laboratory of Biochip Research, City University of Hong Kong, Shenzhen 518057, PR China. Electronic address: bhmyang@cityu.edu.hk.
M S
MS
Yang
D000818:Animals; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D008297:Male; D009990:Oryzias / Q000235:genetics / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012098:Reproduction / Q000187:drug effects; D012737:Sex Factors; D013737:Testis / Q000378:metabolism; D014819:Vitellogenins / Q000378:metabolism
D055768:Halogenated Diphenyl Ethers; D014819:Vitellogenins; C511295:2,2',4,4'-tetrabromodiphenyl ether
BDE-47; Gonads; Marine medaka; Proteomics