Enhanced conjugation of Candida rugosa lipase onto multiwalled carbon nanotubes using reverse micelles as attachment medium and application in nonaqueous biocatalysis.
Three liquid phases (viz. aqueous, nonaqueous, and reverse micelles) were scrutinized as medium for attachment of the enzyme Candida rugosa lipase (CRL) onto multiwalled carbon nanotubes (CNTs). The nanotubes were functionalized to attain carboxyl and amino groups on their surfaces before enzyme conjugation. Transmission electron microscopy and Fourier transformation infrared spectroscopic studies were used for characterization of the nanotubes during the course of functionalization. High enzyme loadings associated with the functionalized CNTs were observed when reverse micelles were used as the attachment medium. In addition, high activity in terms of ester synthesis in organic solvents was also observed while using those preparations. The nanobioconjugates prepared using reverse micelles were found to be highly sturdy and exhibited appreciable operational stability of around 95 ± 3% at 20th cycle (in case of carboxylated nanotubes) and 90 ± 5% at 10th cycle (in case of aminated nanotubes) for esterification. This shows the potential application of reverse micelles as the attachment medium for surface active enzymes such as CRL onto CNTs.
BRD School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, 388 120, Gujarat, India.
Tripti
T
Raghavendra
Uzma
U
Vahora
Amita R
AR
Shah
Datta
D
Madamwar
D055162:Biocatalysis; D002175:Candida / Q000201:enzymology; D002942:Circular Dichroism; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D008823:Micelles; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012997:Solvents; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry
D004800:Enzymes, Immobilized; D008823:Micelles; D037742:Nanotubes, Carbon; D012997:Solvents; D014867:Water; D008049:Lipase
Candida rugosa lipase; multiwalled carbon nanotubes; pentyl valerate; reverse micelles