Solirubrobacter taibaiensis sp. nov., isolated from a stem of Phytolacca acinosa Roxb.
A white-coloured bacterium, designated strain GTJR-20(T), was isolated from a stem of Phytolacca acinosa Roxb. collected from Taibai Mountain in Shaanxi Province, north-west China, and was subjected to a taxonomic study by using a polyphasic approach. The novel isolate was found to grow optimally at 28-30 °C, at pH 7.5-8.0 and in the absence of NaCl. Cells were observed to be Gram-stain positive, strictly aerobic, rod-shaped and non-motile. The predominant respiratory quinone was identified as MK-7(H4) and the major cellular fatty acids were identified as iso-C16:0 (35.8 %), C18:1 ω9c (17.7 %), C17:1 ω6c (11.0 %), C17:1 ω8c (7.8 %) and C18:3 ω6c (6, 9, 12) (7.2 %). The DNA G+C content was determined to be 71.6 mol %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GTJR-20(T) is a member of the genus Solirubrobacter and is closely related to Solirubrobacter phytolaccae GTGR-8(T) (16S rRNA gene sequence similarity, 98.4 %), Solirubrobacter soli KCTC 12628(T) (97.8 %), Solirubrobacter pauli KCTC 9974(T) (97.7 %) and Solirubrobacter ginsenosidimutans KCTC 19420(T) (97.6 %). No other recognized bacterial species showed more than 94.6 % 16S rRNA gene sequence similarity to the novel isolate. DNA-DNA relatedness values for strain GTJR-20(T) with respect to its closely related neighbours S. phytolaccae GTGR-8(T), S. soli KCTC 12628(T), S. pauli KCTC 9974(T) and S. ginsenosidimutans KCTC 19420(T) were 48.3 ± 8.6, 21.3 ± 5.2, 36.8 ± 6.2 and 36.0 ± 5.5 %, respectively. Based on the phenotypic, phylogenetic and genotypic data, strain GTJR-20(T) is considered to represent a novel species of the genus Solirubrobacter, for which the name Solirubrobacter taibaiensis sp. nov. is proposed. The type strain is GTJR-20(T) (=CCTCC AB 2013308(T) = KCTC 29222(T)).
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Lei
L
Zhang
Lingfang
L
Zhu
Meiru
M
Si
Changfu
C
Li
Liang
L
Zhao
Yahong
Y
Wei
Xihui
X
Shen
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D029603:Phytolacca / Q000382:microbiology*; D018547:Plant Stems / Q000382:microbiology; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride