Human mesenchymal cells from adipose tissue deposit laminin and promote regeneration of injured spinal cord in rats.
Cell therapy is a promising strategy to pursue the unmet need for treatment of spinal cord injury (SCI). Although several studies have shown that adult mesenchymal cells contribute to improve the outcomes of SCI, a description of the pro-regenerative events triggered by these cells is still lacking. Here we investigated the regenerative properties of human adipose tissue derived stromal cells (hADSCs) in a rat model of spinal cord compression. Cells were delivered directly into the spinal parenchyma immediately after injury. Human ADSCs promoted functional recovery, tissue preservation, and axonal regeneration. Analysis of the cord tissue showed an abundant deposition of laminin of human origin at the lesion site and spinal midline; the appearance of cell clusters composed of neural precursors in the areas of laminin deposition, and the appearance of blood vessels with separated basement membranes along the spinal axis. These effects were also observed after injection of hADSCs into non-injured spinal cord. Considering that laminin is a well-known inducer of axonal growth, as well a component of the extracellular matrix associated to neural progenitors, we propose that it can be the paracrine factor mediating the pro-regenerative effects of hADSCs in spinal cord injury.
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Karla
K
Menezes
Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Marcos Assis
MA
Nascimento
Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Juliana Pena
JP
Gonçalves
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Aline Silva
AS
Cruz
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Daiana Vieira
DV
Lopes
National Institute of Cancer, Rio de Janeiro, Rio de Janeiro, Brazil.
Bianca
B
Curzio
National Institute of Cancer, Rio de Janeiro, Rio de Janeiro, Brazil.
Martin
M
Bonamino
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
João Ricardo Lacerda
JR
de Menezes
Excellion, Petrópolis, Rio de Janeiro, Brazil.
Radovan
R
Borojevic
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Maria Isabel Doria
MI
Rossi
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Tatiana
T
Coelho-Sampaio
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001253:Astrocytes / Q000166:cytology; D001522:Behavior, Animal; D042783:Endothelial Cells / Q000166:cytology; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006801:Humans; D007249:Inflammation; D007797:Laminin / Q000378:metabolism*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009416:Nerve Regeneration; D009474:Neurons / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D013116:Spinal Cord / Q000473:pathology; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology*
D007797:Laminin