Formate oxidation-driven calcium carbonate precipitation by Methylocystis parvus OBBP.
Microbially induced carbonate precipitation (MICP) applied in the construction industry poses several disadvantages such asammonia release to the air and nitric acid production. An alternative MICP from calcium formate by Methylocystis parvus OBBP is presented here to overcome these disadvantages. To induce calcium carbonate precipitation, M. parvus was incubated at different calcium formate concentrations and starting culture densities. Up to 91.4% ± 1.6% of the initial calcium was precipitated in the methane-amended cultures compared to 35.1% ± 11.9% when methane was not added. Because the bacteria could only utilize methane for growth, higher culture densities and subsequently calcium removals were exhibited in the cultures when methane was added. A higher calcium carbonate precipitate yield was obtained when higher culture densities were used but not necessarily when more calcium formate was added. This was mainly due to salt inhibition of the bacterial activity at a high calcium formate concentration. A maximum 0.67 ± 0.03 g of CaCO3 g of Ca(CHOOH)2(-1) calcium carbonate precipitate yield was obtained when a culture of 10(9) cells ml(-1) and 5 g of calcium formate liter(-)1 were used. Compared to the current strategy employing biogenic urea degradation as the basis for MICP, our approach presents significant improvements in the environmental sustainability of the application in the construction industry.
Giovanni
G
Ganendra
Willem
W
De Muynck
Adrian
A
Ho
Eleni Charalampous
EC
Arvaniti
Baharak
B
Hosseinkhani
Jose Angel
JA
Ramos
Hubert
H
Rahier
Nico
N
Boon
D002119:Calcium Carbonate / Q000737:chemistry* / Q000378:metabolism*; D011232:Chemical Precipitation; D008697:Methane / Q000378:metabolism; D042083:Methylocystaceae / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D010084:Oxidation-Reduction
D002119:Calcium Carbonate; D008697:Methane