Networks development between nicotinic chemical probes and Ca9-22 oral cancer cells by general proteomics analyses.
Tobacco includes thousands of chemicals such as nicotine, which causes numerous diseases including oral cancer. We synthesized nicotinic acid based probes by chemical modification to identify the proteins expressed by the oral cancer cell line Ca9-22 that interact with the nicotinic functional group. Proteins belonging to human oral squamous cell carcinoma were pulled down by a probe carrier based on nicotinic acid, which was reacted with 3-aminopropyltriethoxysilane to compose nicotinic acid linked 3-aminopropyltriethoxysilane exposed on the SiO2 surface. Oral cancer cell lysates were incubated with the nicotinic acid chemical probes to identify the interactions between the nicotinic group and oral cancer cell line extracted proteins. The interactions between the chemical probes and proteins identified as their targets were confirmed by consulting chemicals databases. Interestingly, chaperone proteins (e.g., heat-shock proteins and endoplasmin) that were found to interact with nicotinic acid were identified as binding partners in ribosomal and nucleosome assembly complexes.
Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
Ruei-Nian
RN
Li
Chin-Jen
CJ
Wu
Zong-Jing
ZJ
Yu
Hsueh-Wei
HW
Chang
Shih-Shin
SS
Liang
D014408:Biomarkers, Tumor / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D015336:Molecular Probe Techniques; D009062:Mouth Neoplasms / Q000737:chemistry / Q000378:metabolism*; D009525:Niacin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D060066:Protein Interaction Maps / Q000502:physiology*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics
D014408:Biomarkers, Tumor; D020543:Proteome; D009525:Niacin
Ca9-22; Chemical probes; Chemical-protein interaction; Human oral squamous cell carcinoma; Nicotinic acid probes; Proteomics