Characteristics and enzymatic hydrolysis of cellulose-rich fractions from steam exploded and sequentially alkali delignified bamboo (Phyllostachys pubescens).
In this study, cellulose-rich fractions from bamboo were prepared with steam explosion pretreatment (SEP) followed by a successive alkaline delignification to improve the enzymatic digestibility for an efficient bioethanol production. The cellulose-rich fractions obtained were characterized by FT-IR, XRD, CP/MAS (13)C NMR, SEM, and BET surface area. It was found that the SEP alone significantly removed partial hemicelluloses, while the synergistic treatment by SEP and alkaline delignification removed most hemicelluloses and lignin. Results from enzymatic hydrolysis showed that SEP alone improved the enzymatic hydrolysis rate by 7.9-33.1%, while the synergistic treatment by SEP and alkaline delignification enhanced the rate by 45.7-63.9%. The synergistic treatment by SEP at 2.0 MPa for 5 min with water impregnation followed by a successive alkaline delignification with 0.5% NaOH and 70% ethanol containing 1.5% NaOH resulted in a maximum enzymatic hydrolysis rate of 70.6%.
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
Shao-Ni
SN
Sun
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
Xue-Fei
XF
Cao
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
Xue-Ming
XM
Zhang
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
Feng
F
Xu
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address: rcsun3@bjfu.edu.cn.
Run-Cang
RC
Sun
School of Natural Science, University of Wales, Bangor LL57 2UW, Gwynedd, Wales, UK.
Gwynn Lloyd
GL
Jones
D000468:Alkalies / Q000378:metabolism*; D031723:Bambusa / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D006868:Hydrolysis; D001617:beta-Glucosidase / Q000378:metabolism*
D000468:Alkalies; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase
Alkaline delignification; Bamboo; Characteristics; Enzymatic hydrolysis; Steam explosion