Selective effect of allopregnanolone on corticoliberin-induced anxiety.
We studied the effect of allopregnanolone on stress-induced anxiety caused by intranasal administration of corticoliberin to male Wistar rats. Active and passive rats were selected by their T-maze behavior and then were tested in elevated plus maze for initial anxiety level. Basing on test results, the animals were divided into following groups: active high-anxiety rats, active low-anxiety rats, and passive animals. Rats of the experimental subgroups received subcutaneous injection of allopregnanolone (0.2 mg/kg body weight), control animals received saline. In 30 min, all animals intranasally received corticoliberin (0.5 μg into each nostril) and then were tested in elevated plus maze. Passive animals demonstrated increased anxiety after corticoliberin administration against the background of allopregnanolone. Under the same conditions, low-anxiety rats demonstrated reduced anxiety, while in active high-anxiety animals, only an increase of motor activity was observed. Hence, the effect of allopregnanolone on anxiety level under stress conditions depends on individual typologic features of animal behavior.
Laboratory for Neuroendocrinology, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia, genolsem@mail.ru.
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D000777:Anesthetics / Q000627:therapeutic use*; D000818:Animals; D001007:Anxiety / Q000188:drug therapy*; D001522:Behavior, Animal / Q000187:drug effects; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D011280:Pregnanolone / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000139:chemically induced / Q000188:drug therapy*
D000777:Anesthetics; D003346:Corticotropin-Releasing Hormone; D011280:Pregnanolone