Characterization and cancer cell targeted imaging properties of human antivascular endothelial growth factor monoclonal antibody conjugated CdTe/ZnS quantum dots.
High luminescence quantum yield water-soluble CdTe/ZnS core/shell quantum dots (QDs) stabilized with thioglycolic acid were synthesized. QDs were chemically coupled to fully humanized antivascular endothelial growth factor165 monoclonal antibodies to produce fluorescent probes. These probes can be used to assay the biological affinity of the antibody. The properties of QDs conjugated to an antibody were characterized by ultraviolet and visible spectrophotometry, fluorescent spectrophotometry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transmission electron microscopy and fluorescence microscopy. Cell-targeted imaging was performed in human breast cancer cell lines. The cytotoxicity of bare QDs and fluorescent probes was evaluated in the MCF-7 cells with an MTT viability assay. The results proved that CdTe/ZnS QD-monoclonal antibody nanoprobes had been successfully prepared with excellent spectral properties in target detections. Surface modification by ZnS shell could mitigate the cytotoxicity of cadmium-based QDs. The therapeutic effects of antivascular endothelial growth factor antibodies towards cultured human cancer cells were confirmed by MTT assay.
Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 210009, People's Republic of China.
Lili
L
Pang
Jian
J
Xu
Chang
C
Shu
Jin
J
Guo
Xiaona
X
Ma
Yu
Y
Liu
Wenying
W
Zhong
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D001943:Breast Neoplasms / Q000473:pathology*; D002104:Cadmium / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003952:Diagnostic Imaging / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D045663:Quantum Dots / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013691:Tellurium / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000276:immunology*; D017967:Zinc Compounds / Q000737:chemistry
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D018036:Selenium Compounds; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D017967:Zinc Compounds; D002104:Cadmium; D013691:Tellurium; C044696:zinc selenide
cell imaging; cytotoxicity; human anti-VEGF165 antibody; quantum dots