Structural alteration of cell wall pectins accompanies pea development in response to cold.
Pea (Pisum sativum) cell wall metabolism in response to chilling was investigated in a frost-sensitive genotype 'Terese' and a frost-tolerant genotype 'Champagne'. Cell walls isolated from stipules of cold acclimated and non-acclimated plants showed that cold temperatures induce changes in polymers containing xylose, arabinose, galactose and galacturonic acid residues. In the tolerant cultivar Champagne, acclimation is accompanied by increases in homogalacturonan, xylogalacturonan and highly branched Rhamnogalacturonan I with branched and unbranched (1→5)-α-arabinans and (1→4)-β-galactans. In contrast, the sensitive cultivar Terese accumulates substantial amounts of (1→4)-β-xylans and glucuronoxylan, but not the pectins. Greater JIM7 labeling was observed in Champagne compared to Terese, indicating that cold acclimation also induces an increase in the degree of methylesterification of pectins. Significant decrease in polygalacturonase activities in both genotypes were observed at the end of cold acclimation. These data indicate a role for esterified pectins in cold tolerance. The possible functions for pectins and their associated arabinans and galactans in cold acclimation are discussed.
EA 3900-BIOPI, Biologie des Plantes et Innovation, Université de Picardie Jules Verne, 80039 Amiens, France. Electronic address: lbal@plen.ku.dk.
Laëtitia
L
Baldwin
EA 3900-BIOPI, Biologie des Plantes et Innovation, Université de Picardie Jules Verne, 80039 Amiens, France. Electronic address: jean-marc.domon@u-picardie.fr.
Jean-Marc
JM
Domon
Department of Botany & Plant Pathology, Purdue University, 915 West State Street, West Lafayette, IN 47907-2054, United States. Electronic address: jfklimek@purdue.edu.
John F
JF
Klimek
EA 3900-BIOPI, Biologie des Plantes et Innovation, Université de Picardie Jules Verne, 80039 Amiens, France. Electronic address: francoise.fournet@u-picardie.fr.
Françoise
F
Fournet
INRA USTL UMR 1281, Laboratoire de Génétique et d'Amélioration des Plantes, Estrées-Mons BP50136, 80203 Péronne, France. Electronic address: sellier@mons.inra.fr.
Hélène
H
Sellier
EA 3900-BIOPI, Biologie des Plantes et Innovation, Université de Picardie Jules Verne, 80039 Amiens, France. Electronic address: francoise.gillet@u-picardie.fr.
Françoise
F
Gillet
EA 3900-BIOPI, Biologie des Plantes et Innovation, Université de Picardie Jules Verne, 80039 Amiens, France. Electronic address: jerome.pelloux@u-picardie.fr.
Jérôme
J
Pelloux
INRA USTL UMR 1281, Laboratoire de Génétique et d'Amélioration des Plantes, Estrées-Mons BP50136, 80203 Péronne, France. Electronic address: lejeune@mons.inra.fr.
Isabelle
I
Lejeune-Hénaut
Department of Botany & Plant Pathology, Purdue University, 915 West State Street, West Lafayette, IN 47907-2054, United States. Electronic address: carpita@purdue.edu.
Nicholas C
NC
Carpita
EA 3900-BIOPI, Biologie des Plantes et Innovation, Université de Picardie Jules Verne, 80039 Amiens, France. Electronic address: catherine.rayon@u-picardie.fr.
Catherine
C
Rayon
D000064:Acclimatization; D002473:Cell Wall / Q000201:enzymology / Q000378:metabolism*; D003080:Cold Temperature; D004951:Esterification; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D009005:Monosaccharides / Q000378:metabolism; D018532:Pisum sativum / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D010368:Pectins / Q000378:metabolism*; D010641:Phenotype; D013045:Species Specificity; D014990:Xylans / Q000378:metabolism
D009005:Monosaccharides; D014990:Xylans; C038910:glucuronoxylan; D010368:Pectins
Cell-wall; Cold; Pea; Pectic epitopes; Pectin; Pectin acetylesterase; Pectin methylesterase; Polygalacturonase