Randomly arrayed G-quadruplexes for label-free and real-time assay of enzyme activity.
Randomly arrayed G-quadruplexes can serve as an efficient peroxidase-mimic DNAzyme and provide a novel and facile method to detect terminal deoxynucleotidyl transferase (TdT). Moreover, this G-rich sequence binding to the thioflavin T (ThT) dye can be applied in real-time fluorescent detection of TdT activity.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. niezhou.hnu@gmail.com.
Zhuoliang
Z
Liu
Wang
W
Li
Zhou
Z
Nie
Feifei
F
Peng
Yan
Y
Huang
Shouzhuo
S
Yao
D052160:Benzothiazoles; D001681:Biological Assay; D015374:Biosensing Techniques; D004253:DNA Nucleotidylexotransferase / Q000032:analysis*; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D054856:G-Quadruplexes; D006427:Hemin / Q000737:chemistry / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism*
D052160:Benzothiazoles; D021881:DNA, Catalytic; D013844:Thiazoles; C009462:thioflavin T; D006427:Hemin; D009195:Peroxidase; D004253:DNA Nucleotidylexotransferase