Detailed scrutiny of the anion receptor pocket in subdomain IIA of serum proteins toward individual response to specific ligands: HSA-pocket resembles flexible biological slide-wrench unlike BSA.
Present study reveals that the subdomain IIA cavity of two homologous serum albumins (HSA, BSA) has inherent mutual structural and functional deviations which render noticeable difference in behavior toward specific ligands. The major drug binding site (subdomain IIA) of HSA is found to be largely hydrophobic while that of BSA is partially exposed to water. Larger shift in REE spectra and greater change in solvent reorganization energy of coumarin 343 (C343)-anion in HSA clearly reveals that binding pocket is relatively large and water molecules penetrate deeper into it unlike BSA. The individual response of proteins to perturbation by ligands is found to be way different. Although the subdomain IIA is primarily anion receptive (prefers anionic ligands), the present study suggests that HSA may also like to bind neutral guests due to its remarkable conformational features. Actually, HSA is capable of adopting favorable conformation like mechanical slide-wrench, when required, to accommodate neutral ligands [e.g., coumarin 314 (C314)], as well. But due to less flexible solution structure, BSA behaves like fixed mechanical spanners and hence is not very responsive to C314. Therefore, the generally speaking functional-structural similarities of homologous proteins can be apparent and needs to be analyzed exhaustively.
Department of Chemistry, Indian Institute of Technology Kharagpur , Kharagpur 721302, India.
Shubhashis
S
Datta
Mintu
M
Halder
D000838:Anions / Q000737:chemistry*; D016880:Anisotropy; D002942:Circular Dichroism; D003374:Coumarins / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D062105:Molecular Docking Simulation; D011487:Protein Conformation; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D013395:Sucrose / Q000737:chemistry; D013816:Thermodynamics; D014783:Viscosity; D014867:Water / Q000737:chemistry
D000838:Anions; D003374:Coumarins; D000644:Quaternary Ammonium Compounds; D012709:Serum Albumin; D012997:Solvents; C552279:coumarin 343; D014867:Water; D012710:Serum Albumin, Bovine; D012965:Sodium Chloride; D013395:Sucrose; C009405:tetrabutylammonium; C520890:coumarin 314