The application of a carrier-based bioremediation strategy for marine oil spills.
The application of recycled marine materials to develop sustainable remediation technologies in marine environment was assessed. The remediation strategy consisted of a shell carrier mounted bacterial consortium composed of hydrocarbonoclastic strains enriched with nutrients (Bioaug SC). Pilot scale studies (5000 l) were used to examine the ability of Bioaug-SC to degrade weathered crude oil (10 g l(-1); initially 315,000±44,000 mg l(-1)) and assess the impacts of the introduction and biodegradation of oil. Total petroleum hydrocarbon mass was effectively reduced by 53.3 (±5.75)% to 147,000 (±21,000) mg l(-1) within 27 weeks. 16S rDNA bacterial community profiling using Denaturant Gradient Gel Electrophoresis revealed that cyanobacteria and Proteobacteria dominated the microbial community. Aquatic toxicity assessment was conducted by ecotoxicity assays using brine shrimp hatchability, Microtox and Phaeodactylum tricornutum. This study revealed the importance of combining ecotoxicity assays with oil chemistry analysis to ensure safe remediation methods are developed.
School of Applied Science, Royal Melbourne Institute of Technology (RMIT) University, Bundoora, Victoria 3083, Australia; School of Biological Sciences, Flinders University of South Australia, Adelaide, South Australia 5042, Australia. Electronic address: Petra.Sheppard@rmit.edu.au.
Petra J
PJ
Sheppard
School of Biological Sciences, Flinders University of South Australia, Adelaide, South Australia 5042, Australia.
Keryn L
KL
Simons
School of Applied Science, Royal Melbourne Institute of Technology (RMIT) University, Bundoora, Victoria 3083, Australia; School of Biological Sciences, Flinders University of South Australia, Adelaide, South Australia 5042, Australia.
Eric M
EM
Adetutu
School of Applied Science, Royal Melbourne Institute of Technology (RMIT) University, Bundoora, Victoria 3083, Australia; School of Biological Sciences, Flinders University of South Australia, Adelaide, South Australia 5042, Australia.
Krishna K
KK
Kadali
Centre for Environmental Risk Assessment and Remediation, University of South Australia, Mawson Lakes, South Australia 5095, Australia.
Albert L
AL
Juhasz
Centre for Marine Bio Innovation, University of New South Wales, Sydney, New South Wales, Australia.
Mike
M
Manefield
Environmental and Industrial Biotechnology, The Energy and Resources Institute, Habitat Place, Lodhi Road, New Delhi 100 003, India.
Priyangshu M
PM
Sarma
Environmental and Industrial Biotechnology, The Energy and Resources Institute, Habitat Place, Lodhi Road, New Delhi 100 003, India.
Banwari
B
Lal
School of Applied Science, Royal Melbourne Institute of Technology (RMIT) University, Bundoora, Victoria 3083, Australia; School of Biological Sciences, Flinders University of South Australia, Adelaide, South Australia 5042, Australia.
Andrew S
AS
Ball
D000818:Animals; D001156:Artemia; D001673:Biodegradation, Environmental; D001681:Biological Assay; D000458:Cyanobacteria / Q000378:metabolism; D010578:Petroleum / Q000032:analysis / Q000378:metabolism*; D059392:Petroleum Pollution; D020560:Proteobacteria / Q000378:metabolism; D012623:Seawater / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000633:toxicity
D010578:Petroleum; D014874:Water Pollutants, Chemical
Biodegradation; Carrier material; DGGE; Ecotoxicity; Hydrocarbon