Whole mount immunolabeling of olfactory receptor neurons in the Drosophila antenna.
Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this information to the brain. As such, determining how olfactory information is transferred to the brain, modifying both perception and behavior, merits investigation. Interestingly, there is emerging evidence that cellular transduction and transcriptional factors are involved in the diversification of olfactory receptor neuron. Here we provide a robust whole mount immunological labeling method to assay in vivo olfactory receptor neuron organization. Using this method, we identified all olfactory receptor neurons with anti-ELAV antibody, a known pan-neural marker and Or49a-mCD8::GFP, an olfactory receptor neuron specifically expressed in Nba neuron using anti-GFP antibody.
Laboratory for Genetic Code, Graduate School of Life and Medical Sciences, Doshisha University.
M Rezaul
MR
Karim
Laboratory for Circuit Mechanisms of Sensory Perception, RIKEN Brain Science Institute.
Keita
K
Endo
Disease Mechanism Research Core, RIKEN Brain Science Institute.
Adrian W
AW
Moore
Laboratory for Genetic Code, Graduate School of Life and Medical Sciences, Doshisha University; hitanigu@mail.doshisha.ac.jp.
Hiroaki
H
Taniguchi
D000818:Animals; D057506:Arthropod Antennae / Q000737:chemistry* / Q000166:cytology*; D004330:Drosophila; D007158:Immunologic Techniques / Q000379:methods*; D018034:Olfactory Receptor Neurons / Q000737:chemistry* / Q000166:cytology*