Effects of the modified Huanglian Jiedu decoction on the disease resistance in grey mullet (Mugil cephalus) to Lactococcus garvieae.
Lactococcosis is prevalent on grey mullet (Mugil cephalus) in Hong Kong aquaculture resulting in serious economic loss. A compound formulation of Traditional Chinese Medicines (TCM) (modified Huanglian Jiedu decoction (HLJDD)) comprising Rhizoma coptidis, Radix scutellaria, Cortex phellodendri, Fructus gardeniae, Fructus forsythiae and Flos lonicerae japonicae (in a ratio of 3:2:2:3:3:5) were applied as feed supplements to deal with the disease. The Nitroblue tetrazolium activity in blood, bactericidal activity and total immunoglobulin in plasma were significantly enhanced after feeding 1% of this TCM for 28 days. The disease resistances to Lactococcus garvieae in 1% and 2% TCM feeding groups were significantly enhanced. In the in vitro study, the modified HLJDD also activated the plasma bactericidal activities (p<0.01). Based on this study, 1% modified HLJDD feeding for 28 days may be an optimal dose to prevent L. garvieae infection and could be used in aquaculture industries.
State Key Laboratory in Marine Pollution - Croucher Institute for Environmental Sciences, Hong Kong Baptist University and City University of Hong Kong, Hong Kong, PR China.
W M
WM
Choi
State Key Laboratory in Marine Pollution - Croucher Institute for Environmental Sciences, Hong Kong Baptist University and City University of Hong Kong, Hong Kong, PR China.
C L
CL
Lam
State Key Laboratory in Marine Pollution - Croucher Institute for Environmental Sciences, Hong Kong Baptist University and City University of Hong Kong, Hong Kong, PR China.
W Y
WY
Mo
State Key Laboratory in Marine Pollution - Croucher Institute for Environmental Sciences, Hong Kong Baptist University and City University of Hong Kong, Hong Kong, PR China.
Z
Z
Cheng
State Key Laboratory in Marine Pollution - Croucher Institute for Environmental Sciences, Hong Kong Baptist University and City University of Hong Kong, Hong Kong, PR China.
N K
NK
Mak
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, PR China.
Z X
ZX
Bian
State Key Laboratory in Marine Pollution - Croucher Institute for Environmental Sciences, Hong Kong Baptist University and City University of Hong Kong, Hong Kong, PR China; Centre for Education in Environmental Sustainability and Department of Science and Environmental Studies, The Hong Kong Institute of Education, Hong Kong, PR China. Electronic address: minghwong@ied.edu.hk.
M H
MH
Wong
D000818:Animals; D017756:Aquaculture; D060467:Disease Resistance / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control*; D006723:Hong Kong; D006801:Humans; D017045:Lactococcus / Q000276:immunology*; D008517:Phytotherapy; D017747:Seafood; D023701:Smegmamorpha / Q000097:blood / Q000276:immunology*
D004365:Drugs, Chinese Herbal; C494417:huanglian; C061676:oren gedoku to
Aquaculture; Bactericidal activity; Grey mullet; Lactococcus garvieae; Traditional Chinese Medicine