Substitutional analysis of the C-terminal domain of AbrB revealed its essential role in DNA-binding activity.
The global transition state regulator AbrB controls more than 100 genes of the Bacillus relatives and is known to interact with varying DNA-sequences. The DNA-binding domain of the AbrB-like proteins was proposed to be located exclusively within the amino-terminal ends. However, the recognition of DNA, and specificity of the binding mechanism, remains elusive still in view of highly differing recognition sites. Here we present a substitutional analysis to examine the role of the carboxy-terminal domain of AbrB from Bacillus subtilis and Bacillus amyloliquefaciens. Our results demonstrate that the carboxy-terminal domains of AbrB affect the DNA-binding properties of the tetrameric AbrB. Most likely, the C-termini are responsible for the cooperative character observed for AbrB interaction with some DNA targets like tycA and phyC.
Institute of Biology, Humboldt University of Berlin, Berlin, Germany.
Svetlana
S
Neubauer
Institute of Biology, Humboldt University of Berlin, Berlin, Germany.
Olga
O
Dolgova
Institute of Biology, Humboldt University of Berlin, Berlin, Germany.
Gregory
G
Präg
Institute of Biology, Humboldt University of Berlin, Berlin, Germany.
Rainer
R
Borriss
Institute of Biology, Humboldt University of Berlin, Berlin, Germany; Center for Infectious Diseases and Infection Control, Jena University Hospital, Jena, Germany.
Oliwia
O
Makarewicz
D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics* / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000235:genetics; D010453:Peptide Synthases / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
C060762:AbrB protein, Bacillus subtilis; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D009841:Oligonucleotides; D014157:Transcription Factors; D004247:DNA; D010453:Peptide Synthases; C035180:tyrocidine synthetase