Qualitative and quantitative detection of methylation at CpG sites using the fluorescein-dGTP incorporated asymmetric PCR assay strategy.
The methylation status of each CpG site can be monitored by Fl-dGTP incorporated asymmetric PCR assay. The ability of quantitative detection makes it a good choice for detecting partial methylation at CpG sites compared with others. And the monitoring is not limited to sites within PCR primers or restriction enzyme-recognition sites.
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn.
Tingting
T
Hong
Tianlu
T
Wang
Zhiyong
Z
He
Jingwei
J
Ma
Heng
H
Xiao
Jinguo
J
Huang
Wuxiang
W
Mao
Xiang
X
Zhou
D001681:Biological Assay; D015820:Cadherins / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D017931:DNA Primers; D003848:Deoxyguanine Nucleotides / Q000737:chemistry*; D019793:Fluorescein / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014407:Tumor Cells, Cultured
D015820:Cadherins; D017931:DNA Primers; D003848:Deoxyguanine Nucleotides; C029603:deoxyguanosine triphosphate; D019793:Fluorescein