Polymorphism analysis in genes of the somatotropic axis in Nellore cattle selected for growth.
The somatotropic axis consists of genes that are involved in muscular development. These genes are potential regions of study to identify possible QTL for economically important traits in beef cattle. The aim of this study was to verify the existence of GH1, POU1F1, and GHR polymorphisms in Nellore cattle to verify the influence of selection in these mutations and to analyse the association between molecular markers and body weight at different ages, yearling hip height, carcass fat thickness and loin eye area. Six hundred forty-five animals from the Centro APTA Bovinos de Corte, were genotyped by PCR-RFLP techniques. The association analyses were performed with general mixed models taking into consideration the effect of one marker, and other model taking into consideration interactions between two molecular markers. Only the molecular markers rs81109601 on GH1 and rs109136815 on GHR were polymorphic; however, they were not found to be under selection. The association of the GHR rs109136815 marker and loin eye area was observed (p<0.05), as well as the effect of interaction between the markers and the female body weight at 550 days of age (p<0.04). The interaction effect should be considered in situations where the interactivity between two genes is known.
São Paulo State University, Jaboticabal, SP, Brazil. Electronic address: diercles.cardoso@yahoo.com.br.
Diercles Francisco
DF
Cardoso
São Paulo State University, Jaboticabal, SP, Brazil.
Fábio Ricardo Pablos
FR
de Souza
São Paulo State University, Jaboticabal, SP, Brazil.
Gregório Miguel Ferreira
GM
de Camargo
São Paulo State University, Jaboticabal, SP, Brazil.
Patrícia Dias da Silva
PD
Fonseca
São Paulo State University, Jaboticabal, SP, Brazil.
Larissa Fernanda Simielli
LF
Fonseca
São Paulo State University, Jaboticabal, SP, Brazil.
Camila Urbano
CU
Braz
São Paulo State University, Jaboticabal, SP, Brazil.
Arione Augusti
AA
Boligon
Instituto de Zootecnia, Centro APTA Bovinos de Corte, Sertãozinho, SP, Brazil.
Maria Eugênia Zerlotti
ME
Mercadante
São Paulo State University, Jaboticabal, SP, Brazil.
Lucia Galvão
LG
de Albuquerque
São Paulo State University, Jaboticabal, SP, Brazil.
Humberto
H
Tonhati
D000483:Alleles; D000818:Animals; D002417:Cattle; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D013006:Growth Hormone / Q000235:genetics; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D011986:Receptors, Somatotropin / Q000235:genetics
D011986:Receptors, Somatotropin; D013006:Growth Hormone
GH; GHR; Molecular markers; POU1F1; SNP