Pretreatment of garden biomass by alkali-assisted ultrasonication: effects on enzymatic hydrolysis and ultrastructural changes.
The present investigation aims at studying the effectiveness of alkali-assisted ultrasonication on pretreatment of garden biomass (GB). Dry and powdered GB suspended in 1% NaOH was ultrasonicated for 15, 30 and 60 minutes at a frequency of 25 KHZ. The mode of action and effectiveness of alkali-assisted ultrasonication on GB was established through microscopic, scanning electron microscopic and X-ray diffraction studies. A perusal of results showed that alkali-assisted ultrasonication led to fibrillation of GB which ultimately facilitated enzymatic hydrolysis. The results also indicated that alkali-assisted ultrasonication is an efficient means of pretreatment of GB at moderate (45-50°C) working temperature and low (1%) concentration of alkali. The yield of reducing sugar after enzymatic hydrolysis increased almost six times as compared to control due to alkali-assisted ultrasonication.
Solid and Hazardous Waste Management Division, National Environmental Engineering Research Institute, Nehru marg, Nagpur, Maharashtra, India.
Jagdish
J
Gabhane
Solid and Hazardous Waste Management Division, National Environmental Engineering Research Institute, Nehru marg, Nagpur, Maharashtra, India.
Spm Prince
SP
William
Solid and Hazardous Waste Management Division, National Environmental Engineering Research Institute, Nehru marg, Nagpur, Maharashtra, India.
Atul Narayan
AN
Vaidya
Solid and Hazardous Waste Management Division, National Environmental Engineering Research Institute, Nehru marg, Nagpur, Maharashtra, India.
Duraisamy
D
Anand
Solid and Hazardous Waste Management Division, National Environmental Engineering Research Institute, Nehru marg, Nagpur, Maharashtra, India.
Satish
S
Wate
Alkali pretreatment; Cellulosic ethanol; Enzymatic hydrolysis; Garden biomass; Ultra structural changes; Ultrasonication