Evaluation of antioxidant activity, polyphenolic compounds, amino acids and mineral elements of representative genotypes of Lonicera edulis.
The aim of this study was to evaluate the bioactive substances in 19 berry cultivars of edible honeysuckle (Lonicera edulis). A statistical evaluation was used to determine the relationship between the content of selected bioactive substances and individual cultivars. Regarding mineral elements, the content of sodium was measured using potentiometry and spectrophotometry. The content of selected polyphenolic compounds with high antioxidant activity was determined by a HPLC-UV/ED method. The total amount of polyphenols was determined by the Folin-Ciocalteu method. The antioxidant activity was determined using five methods (DPPH, FRAP, ABTS, FR and DMPD) that differ in their principles. The content of 13 amino acids was determined by ion-exchange chromatography. The experimental results obtained for the different cultivars were evaluated and compared by statistical and bioinformatic methods. A unique feature of this study lies in the exhaustive analysis of the chosen parameters (amino acids, mineral elements, polyphenolic compounds and antioxidant activity) during one growing season.
Department of Viticulture and Enology, Faculty of Horticulture, Mendel University, Valticka 337, CZ-691 44 Lednice, Czech Republic. mojmirbaron@seznam.cz.
Jiri
J
Sochor
Institute for Teacher Training, Faculty of Central European Studies, Constantine the Philosopher University, Drazovska 4, SK-949 74 Nitra, Slovak Republic. tjurikova@ukf.sk.
Tunde
T
Jurikova
Faculty of Military Health Sciences, University of Defence, Trebesska 1575, CZ-500 01 Hradec Kralove, Czech Republic. miroslav.pohanka@gmail.com.
Miroslav
M
Pohanka
Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Kolejni 4, CZ-612 00 Brno, Czech Republic. skutkova@feec.vutbr.cz.
Helena
H
Skutkova
Department of Viticulture and Enology, Faculty of Horticulture, Mendel University, Valticka 337, CZ-691 44 Lednice, Czech Republic. mojmirbaron@seznam.cz.
Mojmir
M
Baron
Department of Viticulture and Enology, Faculty of Horticulture, Mendel University, Valticka 337, CZ-691 44 Lednice, Czech Republic. tomaskova.l.9@gmail.com.
Lenka
L
Tomaskova
Institute for Teacher Training, Faculty of Central European Studies, Constantine the Philosopher University, Drazovska 4, SK-949 74 Nitra, Slovak Republic. sballa@ukf.sk.
Stefan
S
Balla
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Zemedelska 1, CZ-613 00 Brno, Czech Republic. borivoj.klejdus@mendelu.cz.
Borivoj
B
Klejdus
Department of Vegetable Science and Floriculture, Faculty of Horticulture, Mendel University, Lednice, Valtická 337, CZ-691 44 Lednice, Czech Republic. pokluda@mendelu.cz.
Robert
R
Pokluda
Department of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University, Namesti T.G.Masaryka 275, 762 72 Zlin, Czech Republic. mLcek@ft.utb.cz.
Jiri
J
Mlcek
Department of Garden and Landscape Architecture, Faculty of Horticulture, Mendel University, Valticka 337, CZ-691 44 Lednice, Czech Republic. xtrojak2@node.mendelu.cz.
Zuzana
Z
Trojakova
Department of Viticulture and Enology, Faculty of Horticulture, Mendel University, Valticka 337, CZ-691 44 Lednice, Czech Republic. jan_saloun@post.cz.
Jan
J
Saloun
D000596:Amino Acids / Q000032:analysis*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D016000:Cluster Analysis; D005638:Fruit / Q000737:chemistry; D005838:Genotype; D031239:Lonicera / Q000737:chemistry* / Q000235:genetics*; D008903:Minerals / Q000032:analysis; D059808:Polyphenols / Q000032:analysis* / Q000737:chemistry
D000596:Amino Acids; D000975:Antioxidants; D008903:Minerals; D059808:Polyphenols