Synchronous luminescence spectroscopic characterization of urine of normal subjects and cancer patients.
Urine is one of the diagnostically potential bio fluids, as it contains many metabolites and some of them are native fluorophores. These fluorophores distribution and the physiochemical properties may vary during any metabolic change or at different pathologic conditions. Since urine is a multicomponent fluid, synchronous luminescence technique, a powerful tool has been adopted to analyse multicomponents in single spectrum and to resolve emission spectrum without much of photobleaching of fluorophores. In this study, urine samples of both normal subjects and cancer patients were characterised using synchronous luminescence spectroscopy with a Stokes shift of 20 nm. Different ratio parameters were calculated from the intensity values of the synchronous luminescence spectra and they were used as input variables for a multiple linear discriminant analysis across normal and cancer groups. The stepwise linear discriminant analysis classifies 90.3% of the original grouped cases and 88.6% of the cross-validated grouped cases correctly.
Department of Medical Physics, Anna University, Chennai, 600025, India.
Ramu
R
Rajasekaran
Prakasarao
P
Aruna
Dornadula
D
Koteeswaran
Munusamy
M
Baludavid
Singaravelu
S
Ganesan
D000328:Adult; D000368:Aged; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology / Q000652:urine*; D011621:Pteridines / Q000652:urine*; D012256:Riboflavin / Q000652:urine*; D012680:Sensitivity and Specificity; D055815:Young Adult
D011621:Pteridines; D012256:Riboflavin