Association Between Genetic Polymorphism of the MIF Gene and Colorectal Cancer in Taiwan.
BACKGROUND
Colorectal cancer (CRC) is the highest leading cause of cancer-related mortality in Taiwan. Macrophage migration inhibitory factor (MIF) has recently been defined as a novel protumorigenic factor that promotes cell proliferation, migration, and invasion. The aim of the present study is to identify the association between MIF gene polymorphism and CRC.


METHODS
A case-control study was designed to test the hypothesis. A total of 192 biopsy-diagnosed CRC patients (CRC) and 256 healthy subjects (control) were recruited. Genotyping of four single nucleotide polymorphism (SNPs; rs755662, rs11548059, rs1049829, rs1803976) at chromosome positions 755662 (5' UTR), 11548059 (exon2), 1049829 (exon2), 1803976 (exon3) was performed using a Taqman SNP genotyping assay.


RESULTS
There is a significant difference in genotype frequency distribution of rs755662 polymorphism between CRC patients and controls (P = 0.011). No significant difference was found in the frequency distribution of rs11548059, rs1049829, rs1803976 polymorphism in CRC patients and controls (P = 0.660, P = 0.700, and P = 0.959, respectively). Moreover, the MIF-173 SNP was also significantly associated with young patients (age < 50 years, P = 0.026) late stage (Stage IV, P = 0.038) and poor differentiation group (P = 0.040). Compared to the control group, the MIF-173 SNP also significantly associated with patients with stages III and IV (P = 0.034 and 0.003, respectively).


CONCLUSIONS
The presence of MIF-173 (G/C) gene polymorphism (rs755662) was associated with susceptibility, patient age, and stages of CRC in Taiwanese.
Department of Biomedical Informatics, Asia University, Taichung, Taiwan.
Latha
L
Ramireddy
Division of Colorectal Surgery, Department of Surgery, China Medical University Hospital, Taichung, Taiwan.|College of Medicine, China Medical University, Taichung, Taiwan.
William Tzu-Liang
WT
Chen
Department of Biomedical Informatics, Asia University, Taichung, Taiwan.|Department of Pediatrics, Children's Hospital, China Medical University, Taichung, Taiwan.
Ching-Tien
CT
Peng
Department of Biomedical Informatics, Asia University, Taichung, Taiwan.
Rouh-Mei
RM
Hu
Division of Colorectal Surgery, Department of Surgery, China Medical University Hospital, Taichung, Taiwan.
Tao-Wei
TW
Ke
Division of Colorectal Surgery, Department of Surgery, China Medical University Hospital, Taichung, Taiwan.
Hua-Che
HC
Chiang
Division of Colorectal Surgery, Department of Surgery, China Medical University Hospital, Taichung, Taiwan.
Sheng-Chi
SC
Chang
Department of Pediatrics, Children's Hospital, China Medical University, Taichung, Taiwan.
Fuu-Jen
FJ
Tsai
Department of Medical Research, China Medical University Hospital, Taichung, Taiwan.|Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan.|Department of Life Science, National Chung Hsing University, Taichung, Taiwan.
Wan-Yu
WY
Lo
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013624:Taiwan
D008263:Macrophage Migration-Inhibitory Factors; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human
colorectal cancer; genetic polymorphisms; macrophage migration inhibitory factor; susceptibility