Molecular genetic diversity and maternal origin of Chinese black-bone chicken breeds.
Chinese black-bone chickens are valued for the medicinal properties of their meat in traditional Chinese medicine. We investigated the genetic diversity and systematic evolution of Chinese black-bone chicken breeds. We sequenced the DNA of 520 bp of the mitochondrial cyt b gene of nine Chinese black-bone chicken breeds, including Silky chicken, Jinhu black-bone chicken, Jiangshan black-bone chicken, Yugan black-bone chicken, Wumeng black-bone chicken, Muchuan black-bone chicken, Xingwen black-bone chicken, Dehua black-bone chicken, and Yanjin black-bone chicken. We found 13 haplotypes. Haplotype and nucleotide diversity of the nine black-bone chicken breeds ranged from 0 to 0.78571 and 0.00081 to 0.00399, respectively. Genetic diversity was the richest in Jinhu black-bone chickens and the lowest in Yanjin black-bone chickens. Analysis of phylogenetic trees for all birds constructed based on hyplotypes indicated that the maternal origin of black-bone chickens is predominantly from three subspecies of red jungle fowl. These results provide basic data useful for protection of black-bone chickens and help determine the origin of domestic chickens.
Jiangsu Institute of Poultry Science, Yangzhou, Jiangsu, China.
W Q
WQ
Zhu
Animal Science and Technology College, Yangzhou University, Jiangsu, China lhfxf_002@ailiyun.com.
H F
HF
Li
Jiangsu Institute of Poultry Science, Yangzhou, Jiangsu, China.
J Y
JY
Wang
Animal Science and Technology College, Yangzhou University, Jiangsu, China.
J T
JT
Shu
Animal Science and Technology College, Yangzhou University, Jiangsu, China.
C H
CH
Zhu
Animal Science and Technology College, Yangzhou University, Jiangsu, China.
W T
WT
Song
Animal Science and Technology College, Yangzhou University, Jiangsu, China.
C
C
Song
Animal Science and Technology College, Yangzhou University, Jiangsu, China.
G G
GG
Ji
Animal Science and Technology College, Yangzhou University, Jiangsu, China.
H X
HX
Liu
D000818:Animals; D000829:Animals, Domestic / Q000235:genetics; D001947:Breeding; D002645:Chickens / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D014644:Genetic Variation; D006239:Haplotypes; D008460:Meat; D008516:Medicine, Chinese Traditional; D010802:Phylogeny
D004272:DNA, Mitochondrial