Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy.
Liver sinusoidal endothelial cells (LSEC) are an important class of endothelial cells facilitating the translocation of lipoproteins and small molecules between the liver and blood. A number of clinical conditions, especially metabolic and aging-related disorders, are implicated by improper function of LSECs. Despite their importance, research into these cells is limited because the primary ultrastructures involved in their function are transcellular pores, called fenestrations, with diameters in a size range between 50-200 nm, i.e. well below the optical diffraction limit. Here, we show that we are able to resolve fenestrations with a spatial resolution of ∼20 nm by direct stochastic optical reconstruction microscopy (dSTORM). The cellular plasma membrane was labeled at high fluorophore density with CellMask Deep Red and imaged using a reducing buffer system. We compare the higher degree of structural detail that dSTORM provides to results obtained by 3D structured illumination microscopy (3D-SIM). Our results open up a path to image these physiologically important cells in vitro using highly resolving localization microscopy techniques that could be implemented on non-specialized fluorescence microscopes, enabling their investigation in most biomedical laboratories without the need for electron microscopy.
Biomolecular Photonics, Department of Physics, University of Bielefeld, 33615 Bielefeld, Germany. thomas.huser@physik.uni-bielefeld.de.
Viola
V
Mönkemöller
Mark
M
Schüttpelz
Peter
P
McCourt
Karen
K
Sørensen
Bård
B
Smedsrød
Thomas
T
Huser
D000818:Animals; D004727:Endothelium / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008853:Microscopy / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013269:Stochastic Processes