Lysophosphatidic acid signaling in late cleavage and blastocyst stage bovine embryos.
Lysophosphatidic acid (LPA) is a known cell signaling lipid mediator in reproductive tissues. In the cow, LPA is involved in luteal and early pregnancy maintenance. Here, we evaluated the presence and role of LPA in bovine early embryonic development. In relevant aspects, bovine embryos reflect more closely the scenario occurring in human embryos than the mouse model. Transcription of mRNA and protein expression of enzymes involved in LPA synthesis (ATX and cPLA2) and of LPA receptors (LPAR1-4) were detected in Days 5 and 8 in vitro produced embryos. Embryonic LPA production into culture medium was also detected at both stages of development. Supplementation of culture medium with LPA (10(-5) M) between Days 2 and 8 had no effect on embryo yield and quality and on blastocyst relative mRNA abundance of genes involved in prostaglandin synthesis (PTGS2, PGES, and PGFS) and steroidogenesis (3βHSD). However, LPA treatment affected transcription levels of embryo quality markers, decreasing BAX (apoptotic) and increasing BCL2 (antiapoptotic) and IGF2R (growth marker) gene transcription levels. Blastocyst transcription of OCT4 (pluripotency marker) was not affected by LPA stimulation. In conclusion, LPA is an early bovine embryonic autocrine/paracrine signaling mediator, and LPA action may be relevant in early embryo-maternal interactions leading to embryonic survival.
Faculty of Veterinary Medicine, CIISA,University of Lisbon, 1300-477 Lisbon, Portugal.
Ana Catarina
AC
Torres
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
Dorota
D
Boruszewska
Faculty of Veterinary Medicine, CIISA,University of Lisbon, 1300-477 Lisbon, Portugal.
Mariana
M
Batista
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
Ilona
I
Kowalczyk-Zieba
Faculty of Veterinary Medicine, CIISA,University of Lisbon, 1300-477 Lisbon, Portugal.
Patricia
0000-0001-5303-6953
P
Diniz
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
Emilia
E
Sinderewicz
Institute of Metabolic and Cardiovascular Diseases, Inserm U1048, Paul Sabatier University, 31432 Toulouse, France.
Jean Sebastian
JS
Saulnier-Blache
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
Izabela
I
Woclawek-Potocka
Faculty of Veterinary Medicine, CIISA,University of Lisbon, 1300-477 Lisbon, Portugal.
Luis
L
Lopes-da-Costa
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D002417:Cattle; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D008246:Lysophospholipids / Q000494:pharmacology*; D011247:Pregnancy; D015398:Signal Transduction
D008246:Lysophospholipids; D051546:Cyclooxygenase 2; C032881:lysophosphatidic acid