TMPRSS13 deficiency impairs stratum corneum formation and epidermal barrier acquisition.
Membrane-anchored serine proteases serve as important regulators of multiple developmental and homoeostatic processes in mammals. TMPRSS13 (transmembrane protease, serine 13; also known as mosaic serine protease large-form, MSPL) is a membrane-anchored serine protease with unknown biological functions. In the present study, we used mice with the Tmprss13 gene disrupted by a β-galactosidase-neomycin fusion gene insertion to study the expression and function of the membrane-anchored serine protease. High levels of Tmprss13 expression were found in the epithelia of the oral cavity, upper digestive tract and skin. Compatible with this expression pattern, Tmprss13-deficient mice displayed abnormal skin development, leading to a compromised barrier function, as measured by the transepidermal fluid loss rate of newborn mice. The present study provides the first biological function for the transmembrane serine protease TMPRSS13.
*Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
Daniel H
DH
Madsen
*Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
Roman
R
Szabo
*Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
Alfredo A
AA
Molinolo
*Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
Thomas H
TH
Bugge
D000818:Animals; D003433:Crosses, Genetic; D000078404:Epidermal Cells; D004817:Epidermis / Q000196:embryology / Q000378:metabolism* / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006579:Heterozygote; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009061:Mouth Mucosa / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000473:pathology; D009092:Mucous Membrane / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000473:pathology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012697:Serine Endopeptidases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D057057:Serine Proteases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D041742:Upper Gastrointestinal Tract / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000473:pathology; D001743:Urinary Bladder / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000473:pathology; D014882:Water-Electrolyte Balance; D014883:Water-Electrolyte Imbalance / Q000196:embryology / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase; D057057:Serine Proteases; D012697:Serine Endopeptidases; C000591652:Tmprss13 protein, mouse