Fluorescence detection of adenosine-5'-triphosphate and alkaline phosphatase based on the generation of CdS quantum dots.
We have developed an analytical method to detect adenosine-5'-triphosphate (ATP) and alkaline phosphatase (ALP) based on the generation of CdS quantum dots (QDs). We demonstrated that Cd(2+) cation reacts with S(2-) anion to generate fluorescent CdS QDs in the presence of some certain amount of ATP. With increase in the ATP concentration, the fluorescence intensity of CdS QDs was also enhanced. ATP can be converted into adenosine by the dephosphorylation of ALP, so that the generation of CdS QDs would be inhibited in the presence of ALP. Therefore, this novel analysis system could be applied to assay ATP and ALP based on the growth of fluorescent CdS QDs.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Siyu
S
Liu
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Xinyan
X
Wang
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Shu
S
Pang
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Weidan
W
Na
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Xu
X
Yan
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: suxg@jlu.edu.cn.
Xingguang
X
Su
D000244:Adenosine Diphosphate / Q000737:chemistry; D000249:Adenosine Monophosphate / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry; D000469:Alkaline Phosphatase / Q000032:analysis*; D019187:Cadmium Compounds / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D045663:Quantum Dots / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013440:Sulfides / Q000737:chemistry*; D013997:Time Factors
D019187:Cadmium Compounds; D013440:Sulfides; C034939:cadmium sulfide; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase
Adenosine-5′-triphosphate; Alkaline phosphatase; CdS quantum dots; Fluorescence