Metabolites profiling of Pulsatilla saponin D in rat by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS/MS).
Pulsatilla saponin D, an antitumor substance isolated from traditional Chinese herbal medicine Pulsatilla chinensis (Bge.) Regel, is a promising candidate for new drug development. The purpose of the present study is to establish a simple and practical strategy for the metabolite profiling of Pulsatilla saponin D in vivo. A total of 18 metabolites were identified in rat plasma, urine and feces samples based on MS and MS/MS data by using ESI-Q-TOF-MS/MS, and eight of them (M11-M18) were reported for the first time. The results indicated that deglycosylation, dehydrogenation, hydroxylation and sulfation were the major metabolic transformations of Pulsatilla saponin D in vivo. This study has improved our understanding of the metabolic fate of Pulsatilla saponin D in vivo, and the information gained from the current study is relevant to the pharmacological activity of Pulsatilla saponin D.
Jiangxi University of Traditional Chinese Medicine, No. 56 Yangmin Road, Nanchang 330006, PR China; Guangzhou University of Chinese Medicine, No. 232 Waihuandong Road, Guangzhou 510405, PR China.
Hui
H
Ouyang
Jiangxi University of Traditional Chinese Medicine, No. 56 Yangmin Road, Nanchang 330006, PR China.
Maofu
M
Zhou
Jiangxi University of Traditional Chinese Medicine, No. 56 Yangmin Road, Nanchang 330006, PR China.
Yicheng
Y
Guo
Jiangxi University of Traditional Chinese Medicine, No. 56 Yangmin Road, Nanchang 330006, PR China.
Mingzhen
M
He
Jiangxi University of Traditional Chinese Medicine, No. 56 Yangmin Road, Nanchang 330006, PR China.
Hesong
H
Huang
Jiangxi University of Traditional Chinese Medicine, No. 56 Yangmin Road, Nanchang 330006, PR China; Guangzhou University of Chinese Medicine, No. 232 Waihuandong Road, Guangzhou 510405, PR China.
Xide
X
Ye
Jiangxi University of Traditional Chinese Medicine, No. 56 Yangmin Road, Nanchang 330006, PR China. Electronic address: sun17908@126.com.
Yulin
Y
Feng
The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China.
Xin
X
Zhou
Jiangxi University of Traditional Chinese Medicine, No. 56 Yangmin Road, Nanchang 330006, PR China; Guangzhou University of Chinese Medicine, No. 232 Waihuandong Road, Guangzhou 510405, PR China.
Shilin
S
Yang
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005243:Feces / Q000737:chemistry; D006900:Hydroxylation; D008516:Medicine, Chinese Traditional; D053858:Metabolic Networks and Pathways; D031946:Pulsatilla / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004365:Drugs, Chinese Herbal; D012503:Saponins
ESI–Q-TOF-MS/MS; Hederagenin (PubChem CID 73299); Metabolites; Pulsatilla saponin D; Pulsatilla saponin D (PubChem CID 11650910)