Purification and identification of antioxidant peptides from peanut protein isolate hydrolysates using UHR-Q-TOF mass spectrometer.
Peanut protein isolate (PPI) was hydrolysed with alcalase to obtain antioxidant peptides. To purify these peptides, the peanut protein isolate hydrolysates (PPIH) were separated by ultrafiltration (MWCO=3kDa) and the obtained PPIH-II (Mw<3kDa) with higher antioxidant activity was further separated by gel filtration chromatography (Sephadex G-15). After filtration, both peptides, P1 and P4, with stronger antioxidant capacity were fractionated using preparative high performance liquid chromatography (P-HPLC). Three antioxidant peptides were finally purified from P1 and P4 using the UHR-Q-TOF mass spectrometer, and the amino acid sequences of the peptides were identified as Thr-Pro-Ala (286kDa), Ile/Leu-Pro-Ser (315kDa) and Ser-Pro (202kDa), respectively.
School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
Na
N
Ji
School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
Cuixia
C
Sun
School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
Yunxia
Y
Zhao
School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
Liu
L
Xiong
School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China. Electronic address: phdsun@163.com.
Qingjie
Q
Sun
D000595:Amino Acid Sequence; D000975:Antioxidants / Q000737:chemistry*; D010367:Arachis / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011492:Protein Hydrolysates
D000975:Antioxidants; D010455:Peptides; D011492:Protein Hydrolysates
Amino acid sequence; Antioxidant peptides; Identification; Peanut protein isolate hydrolysates