Development of a magnetic 3D spheroid platform with potential application for high-throughput drug screening.
Three-dimensional (3D) cell culture has become increasingly adopted as a more accurate model of the complex in vivo microenvironment compared to conventional two-dimensional (2D) cell culture. Multicellular spheroids are important 3D cell culture models widely used in biological studies and drug screening. To facilitate simple spheroid manipulation, magnetic spheroids were generated from magnetically labeled cells using a scaffold-free approach. This method is applicable to a variety of cell types. The spheroids generated can be targeted and immobilized using magnetic field gradients, allowing media change or dilution to be performed with minimal disruption to the spheroids. Cells in magnetic spheroids showed good viability and displayed typical 3D morphology. Using this platform, a 28 day study was carried out using doxorubicin on magnetic MCF-7 spheroids. The results provided a proof-of-principle for using magnetic tumor spheroids in therapeutic studies. They can offer beneficial insights that help to bridge the gap between in vitro and in vivo models. Furthermore, this platform can be adapted for high-throughput screening in drug discovery.
Molecular Engineering Laboratory, A*STAR, Proteos , 61 Biopolis Drive, Singapore 138673, Singapore.
Wei Mei
WM
Guo
Xian Jun
XJ
Loh
Ern Yu
EY
Tan
Joachim S C
JS
Loo
Vincent H B
VH
Ho
D000970:Antineoplastic Agents / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology; D055808:Drug Discovery / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D045325:HCT116 Cells; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D060328:Magnetic Phenomena; D008280:Magnetics / Q000379:methods*; D018874:Spheroids, Cellular / Q000187:drug effects / Q000502:physiology*
D000970:Antineoplastic Agents; D004317:Doxorubicin