Tim-3 expression is increased on peripheral T cells from diffuse large B cell lymphoma.
T cell immunoglobulin- and mucin-domain-containing molecule 3 (Tim-3) plays a critical role in immune tolerance by suppressing the activation and proliferation of T cells. The purpose of this study was to investigate the effect of Tim-3 on the development of diffuse large B cell lymphoma (DLBCL). A total of 40 newly diagnosed DLBCL patients and 30 healthy donors were recruited. Tim-3 expression on peripheral CD4+ T and CD8+ T cells was analyzed by flow cytometry. Data showed that expression of Tim-3 was significantly increased on both CD4+ and CD8+ T cells in DLBCL patients than in healthy controls (P < 0.001 and P < 0.001, respectively). Also, level of Tim-3 on CD4+ T cells was positively correlated with CD8+ T cells in patients (P < 0.001). Further analyses revealed that patients with advanced tumor stages had elevated Tim-3 expression on CD4+ and CD8+ T cells compared to those with primary tumor stages. In addition, levels of Tim-3 on CD4+ and CD8+ T cells were significantly elevated in DLBCL patients with bone marrow involvement or B symptoms. Our results suggest that Tim-3 is involved in the development of DLBCL.
Department of Hematology, Liaocheng People's Hospital, 67 Dong Chang Xi Road, Liaocheng, 252000, Shandong Province, China, taiwuxiao@126.com.
Taiwu
T
Xiao
Li
L
Zhang
Lei
L
Chen
Guozhen
G
Liu
Zhenjun
Z
Feng
Lei
L
Gao
D000328:Adult; D000368:Aged; D005260:Female; D000072597:Hepatitis A Virus Cellular Receptor 2; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000502:physiology*; D008875:Middle Aged; D009367:Neoplasm Staging; D013601:T-Lymphocytes / Q000378:metabolism*
C448908:HAVCR2 protein, human; D000072597:Hepatitis A Virus Cellular Receptor 2; D008565:Membrane Proteins