Cadmium supplement triggers endoplasmic reticulum stress response and cytotoxicity in primary chicken hepatocytes.
Cadmium (Cd), a potent hepatotoxin, has been reported to induce endoplasmic reticulum (ER) stress in various cell types. However, whether such effect exists in bird is still unclear. To delineate the effects of Cd exposure on ER stress response, we examined the expression of 78-kDa glucose-regulated protein (GRP78) and alteration in calcium homeostasis in primary chicken hepatocytes treated with 2-22 µM Cd for 24 h. A significant decrease of cell viability was observed in chicken hepatocytes following Cd administration. In cells treated with Cd, GRP78 protein levels increased in a dose-dependent manner. In addition, GRP78 and GRP94mRNA levels were elevated in response to Cd exposure. The increase of the intracellular Ca(2+) concentration in chicken hepatocytes was found during Cd exposure. Cd significantly decreased the CaM mRNA levels in hepatocytes. These results show that Cd regulates the expression of GRP78 and calcium homeostasis in chicken hepatocytes, suggesting that ER stress induced by Cd plays an important role in the mechanisms of Cd cytotoxicity to the bird hepatocytes.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People׳s Republic of China.
Cheng-Cheng
CC
Shao
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People׳s Republic of China.
Nan
N
Li
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People׳s Republic of China.
Zi-Wei
ZW
Zhang
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People׳s Republic of China.
Jian
J
Su
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People׳s Republic of China.
Shu
S
Li
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People׳s Republic of China. Electronic address: jinlongli@neau.edu.cn.
Jin-Long
JL
Li
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People׳s Republic of China. Electronic address: shiwenxu@neau.edu.cn.
Shi-Wen
SW
Xu
D000818:Animals; D002104:Cadmium / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens / Q000235:genetics / Q000378:metabolism; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D004785:Environmental Pollutants / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D022781:Hepatocytes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D000091342:Endoplasmic Reticulum Chaperone BiP; D004785:Environmental Pollutants; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C018551:glucose-regulated proteins; D002104:Cadmium; D002118:Calcium
Cadmium; Calcium homeostasis; Chicken hepatocytes; Endoplasmic reticulum Stress; Expression of GRP78