Functionalized graphene oxide for the fabrication of paraoxon biosensors.
There is an increasing need to develop biosensors for the detection of harmful pesticide residues in food and water. Here, we report on a versatile strategy to synthesize functionalized graphene oxide nanomaterials with abundant affinity groups that can capture histidine (His)-tagged acetylcholinesterase (AChE) for the fabrication of paraoxon biosensors. Initially, exfoliated graphene oxide (GO) was functionalized by a diazonium reaction to introduce abundant carboxyl groups. Then, Nα,Nα-bis(carboxymethyl)-l-lysine hydrate (NTA-NH2) and Ni(2+) were anchored onto the GO based materials step by step. AChE was immobilized on the functionalized graphene oxide (FGO) through the specific binding between Ni-NTA and His-tag. A low anodic oxidation potential was observed due to an enhanced electrocatalytic activity and a large surface area brought about by the use of FGO. Furthermore, a sensitivity of 2.23 μA mM(-1) to the acetylthiocholine chloride (ATChCl) substrate was found for our composite covered electrodes. The electrodes also showed a wide linear response range from 10 μM to 1mM (R(2)=0.996), with an estimated detection limit of 3 μM based on an S/N=3. The stable chelation between Ni-NTA and His-tagged AChE endowed our electrodes with great short-term and long-term stability. In addition, a linear correlation was found between paraoxon concentration and the inhibition response of the electrodes to paraoxon, with a detection limit of 6.5×10(-10) M. This versatile strategy provides a platform to fabricate graphene oxide based nanomaterials for biosensor applications.
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
Hangyu
H
Zhang
Department of Mechanical Engineering, Purdue School of Engineering and Technology, Indiana University-Purdue University Indianapolis (IUPUI), Indianapolis, IN 46202, USA.
Zhe-fei
ZF
Li
School of Materials Engineering, Purdue University, West Lafayette, IN 47907, USA.
Alexandra
A
Snyder
Department of Mechanical Engineering, Purdue School of Engineering and Technology, Indiana University-Purdue University Indianapolis (IUPUI), Indianapolis, IN 46202, USA.
Jian
J
Xie
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA; School of Materials Engineering, Purdue University, West Lafayette, IN 47907, USA. Electronic address: lstanciu@purdue.edu.
Lia A
LA
Stanciu
D010129:4-Aminobenzoic Acid / Q000737:chemistry; D000110:Acetylcholinesterase / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D003035:Cobalt / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry* / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D057230:Limit of Detection; D008239:Lysine / Q000737:chemistry; D009532:Nickel / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*; D010261:Paraoxon / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*
D004800:Enzymes, Immobilized; D010087:Oxides; D010573:Pesticide Residues; D003035:Cobalt; D006639:Histidine; D006108:Graphite; D009532:Nickel; D000110:Acetylcholinesterase; D008239:Lysine; D010261:Paraoxon; D010129:4-Aminobenzoic Acid
AChE; Affinity; Functionalization; Graphene oxide; Pesticide biosensor