Molecular cloning and mRNA expression analysis of ornithine decarboxylase antizyme 2 in ovarian follicles of the Sichuan white goose (Anser cygnoides).
The ornithine decarboxylase antizyme 2 (OAZ2) gene is a member of the antizyme gene family. Antizymes play pivotal roles in various cellular pathways, including polyamine anabolism and apoptosis. The molecular structure and expression profile of the OAZ2 in goose ovarian follicles have not been reported. In this study, the OAZ2 cDNA sequence of the Sichuan white goose was cloned (Anser cygnoides), and phylogenetic and structural analyses of the OAZ2 were performed. The expression profiling of OAZ2 mRNA in goose ovarian follicles was examined using quantitative real-time PCR. The sequence analysis showed that the 756 bp OAZ2 sequence contained two overlapping open reading frames (ORF). ORF1 was 99 bp in length, and encoded a 32 aa polypeptide. ORF2 was 477 bp in length, and encoded a 158 aa polypeptide. The frameshift site that initiates the translation of ORF2 was located at nucleotide position 97 in the OAZ2. The analysis of OAZ2 mRNA expression in hierarchical follicles showed that the level of OAZ2 mRNA was higher in the SWF and F2 follicular stages than that in the ovarian stroma (P<0.05). The lowest level of OAZ2 expression was detected in the ovarian stroma. These results suggest that the highly conserved frameshift region plays an important role in sustaining the function of OAZs. Furthermore, the significantly higher level of OAZ2 mRNA in the SWF stage indicates that OAZ2 may be involved in recruiting hierarchical follicles. Our results also suggest that OAZ2 may augment the effects of OAZ1 in follicle development.
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, Sichuan Province, China.
Hui
H
He
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, Sichuan Province, China. Electronic address: albertkb119@163.com.
Bo
B
Kang
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, Sichuan Province, China. Electronic address: jiangdm9277@163.com.
Dongmei
D
Jiang
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, Sichuan Province, China.
Rong
R
Ma
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, Sichuan Province, China.
Lin
L
Bai
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D005777:Geese / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D006080:Ovarian Follicle / Q000378:metabolism*; D010802:Phylogeny; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D011506:Proteins; D012333:RNA, Messenger; C031017:ornithine decarboxylase antizyme
Clone; Follicle; Goose; Ornithine decarboxylase antizyme 2; Quantitative real-time PCR