Molecular characterization and sequence analysis of the 2B region of Aichivirus C strains in Japan and Thailand.
Aichivirus C is the third species in the genus Kobuvirus, family Picornaviridae, and the virus is circulating in pigs worldwide. Aichivirus A in humans and Aichivirus B in cows have been shown to associate with diarrheal diseases, however, the pathogenesis of Aichivirus C has not been demonstrated clearly. In this study, the full genome nucleotide sequence of the Thai strain, CMP06/2007/THA collected from stool sample of a diarrheal piglet was analyzed and identified as a variant type with a 90-nt deletion in the 2B-coding region. In addition, molecular characterization of nucleotide sequences of the 2B-coding region of Aichivirus C strains from six diarrheal and six healthy piglets in Thailand, and four strains from healthy pigs in Japan revealed that all of the strains in this study were variant types. These findings indicate that variant strains of Aichivirus C are circulating in Asian countries such as China, Thailand and Japan, and deletion of tandem repeat of 2B-region is unlikely to associate with the pathogenesis of the virus.
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan; Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan. Electronic address: shoko-tky@umin.ac.jp.
Shoko
S
Okitsu
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Pattara
P
Khamrin
Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Aksara
A
Thongprachum
Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Angela F C
AF
Kalesaran
Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Sayaka
S
Takanashi
Department of Virus II, National Institute of Infectious Diseases, Tokyo, Japan.
Hiroyuki
H
Shimizu
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Niwat
N
Maneekarn
Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Masashi
M
Mizuguchi
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Satoshi
S
Hayakawa
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan; Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Hiroshi
H
Ushijima
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D005814:Genes, Viral; D016679:Genome, Viral; D007564:Japan / Q000453:epidemiology; D052699:Kobuvirus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000662:veterinary*; D015995:Prevalence; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D013785:Thailand / Q000453:epidemiology
2B gene; Diarrhea; Pig; Serum; Stool; Variant