Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay.
In the present work, we propose a normalised real-time quantitative PCR assay to determine the addition of soybean to meat products. The method proved to be a powerful tool for the quantification of soybean protein (dry basis) in the range of 0.01% to 6%, being successfully in-house validated. Its application was effective in the analysis of several meat products, indicating 2% of non-compliance with the food allergen labelling legislation, and some inconsistencies when comparing the declared with estimated amounts of soybean. This work highlights the importance of efficient tools to assess labelling statements of meat products, avoiding fraudulent practices.
REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
Sónia
S
Soares
REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; ESTiG, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, 5301-857 Bragança, Portugal. Electronic address: jamaral@ipb.pt.
Joana S
JS
Amaral
REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
M Beatriz P P
MB
Oliveira
REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal. Electronic address: isabel.mafra@ff.up.pt.
Isabel
I
Mafra
D000818:Animals; D018744:DNA, Plant / Q000302:isolation & purification*; D023381:Endpoint Determination; D005506:Food Contamination / Q000032:analysis*; D005515:Food Labeling; D008461:Meat Products / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D030262:Soybean Proteins / Q000032:analysis; D013025:Glycine max; D013552:Swine
D018744:DNA, Plant; D030262:Soybean Proteins
Adulteration; Food allergen; Meat products; Real-time PCR; Soybean detection