Development of an immunochromatographic kit for rapid detection of human influenza B virus infection.
BACKGROUND
Type B influenza virus is a major epidemic strain responsible for considerable mortality and morbidity.


METHODS
A colloidal gold immunochromatographic strip for the rapid detection of human influenza B virus was developed. This test is based on membrane chromatography and uses colloidal gold conjugated with influenza B virus anti-NP monoclonal antibody as the tracer. The assembled test strip was housed in a plastic case.


RESULTS
The colloid gold strip (CGS) specifically detected all influenza B viruses tested and did not react with other respiratory viruses. Compared with SYBR Green real-time PCR, the sensitivity and specificity of the CGS test was 89.76% and 99.56%, respectively, and the consistency ratio between CGS and real-time PCR was 96.06% in detecting influenza B virus in 710 nasopharyngeal swabs from patients with influenza-like illness in the hospital.


CONCLUSIONS
The CGS array developed in this study enabled typing of influenza B viruses in human clinical specimens. Thus, together with the advantages of rapid detection and easy operation without requiring specialized personnel and equipment, this technique is a convenient and relatively inexpensive diagnostic tool for large-scale screening of clinical samples.
Peirui
P
Zhang
Yueqiang
Y
Duan
Dexi
D
Zhang
Shaogeng
S
Zhang
Zhiwei
Z
Li
Xiliang
X
Wang
Penghui
P
Yang
D000260:Adenoviruses, Human / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000302:isolation & purification; D009980:Influenza A virus / Q000302:isolation & purification; D009981:Influenza B virus / Q000302:isolation & purification* / Q000472:pathogenicity; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D008875:Middle Aged; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification; D055815:Young Adult