Spatial and temporal expression of RA70/Scap2 in the developing neural tube.
Src kinase-associated phosphoprotein 2 (Ra70/scap2), which was originally isolated as a retinoic acid (RA)-induced gene, associates with molecules that modulate integrin-survival signals. Although RA is essential for vertebrate organogenesis in the posterior region, little is known about the biological role of RA70/Scap2 during development. In the present study, we demonstrate that Ra70/scap2 mRNA is temporally expressed during the RA-induced neuronal differentiation of P19 embryonic carcinoma cells. Homozygous knockout mice in which the Ra70/scap2 gene was replaced with LacZ exhibited embryonic lethality, while heterozygous mice displayed preferential expression of LacZ in posterior neural tissues, including the neural tube and hindbrain during development (E7.5-11.5), but not the forebrain. Ra70/scap2 was expressed in the ependymal layer and ventricular zone in the neural tube, where neuroepithelial cells and neuroblasts with proliferation capacity are localized, respectively. Thus, RA70/Scap2 may be necessary for RA-induced neuronal differentiation from the posterior neuroectoderm.
Center for Medical Science, International University of Health and Welfare, Kitakanemaru, Ohtawara, Tochigi, Japan.
Yuko
Y
Tanabe
PhoenixBio, Ltd., Iwazo, Utsunomiya, Tochigi, Japan.
Akira
A
Shiota
Center for Medical Science, International University of Health and Welfare, Kitakanemaru, Ohtawara, Tochigi, Japan.
Yoriko
Y
Kouroku-Murakami
Center for Medical Science, International University of Health and Welfare, Kitakanemaru, Ohtawara, Tochigi, Japan; Department of Pediatrics, Jichi Medical University, Yakushiji, Shimotsukeshi, Tochigi, Japan.
Eriko
E
Fujita-Jimbo
Department of Biology, School of Medicine Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan.
Koko
K
Urase
Department of Cell Biology and Morphology, Akita University Graduate School of Medicine, Hondo, Akita, Japan.
Kana
K
Takahashi
Department of Cell Biology and Morphology, Akita University Graduate School of Medicine, Hondo, Akita, Japan.
Yoshihiro
Y
Mezaki
Department of Cell Biology and Morphology, Akita University Graduate School of Medicine, Hondo, Akita, Japan.
Haruki
H
Senoo
Center for Medical Science, International University of Health and Welfare, Kitakanemaru, Ohtawara, Tochigi, Japan. Electronic address: momoi@iuhw.ac.jp.
Takashi
T
Momoi
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D018345:Mice, Knockout; D054259:Neural Tube / Q000196:embryology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C116431:src kinase associated phosphoprotein 2
Integrin; Neural tube; Retinoic acid; SKAP55; Scap