Transcriptome analysis of a microbial coculture in which the cell populations are separated by a membrane.
The microbial coculture of multiple cell populations is used to study community evolution and for bioengineering applications. The cells in coculture undergo dynamic changes because of cell-cell and cell-environment interactions. Transcriptome analysis allows us to study the molecular basis of these changes in cell physiology. For transcriptome analysis, it is essential that the cell populations in the coculture are harvested separately. Here, we describe a method for transcriptome analysis of a microbial coculture in which two different cell populations are separated by a porous membrane.
Graduate School of Information Science and Technology, Osaka University, Yamadaoka 1-5, Suita, Osaka, 565-0871, Japan.
Kazufumi
K
Hosoda
Naoaki
N
Ono
Shingo
S
Suzuki
Tetsuya
T
Yomo
D018920:Coculture Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D004926:Escherichia coli / Q000235:genetics*; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D008567:Membranes, Artificial; D008828:Microbiological Techniques / Q000295:instrumentation* / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification
D008567:Membranes, Artificial; D012329:RNA, Bacterial