Differentially instructive extracellular protein micro-nets.
An ability to construct biological matter from the molecule up holds promise for applications ranging from smart materials to integrated biophysical models for synthetic biology. Biomolecular self-assembly is an efficient strategy for biomaterial construction which can be programmed to support desired function. A challenge remains in replicating the strategy synthetically, that is at will, and differentially, that is for a specific function at a given length scale. Here we introduce a self-assembly topology enabling a net-like architectural mimetic of native extracellular matrices capable of differential responses to cell adhesion--enhanced mammalian cell attachment and proliferation, and enhanced resistance to bacterial colonization--at the native sub-millimeter length scales. The biological performance of such protein micro-nets directly correlates with their morphological and chemical properties, offering thus an application model for differential extracellular matrices.
National Physical Laboratory , Hampton Road, Teddington TW11 0LW, U.K.
Nilofar
N
Faruqui
Angelo
A
Bella
Jascindra
J
Ravi
Santanu
S
Ray
Baptiste
B
Lamarre
Maxim G
MG
Ryadnov
D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D002448:Cell Adhesion; D049109:Cell Proliferation; D003500:Cyclization; D003599:Cytoskeleton / Q000737:chemistry / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000138:chemical synthesis* / Q000737:chemistry*; D005347:Fibroblasts / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D008958:Models, Molecular; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D011487:Protein Conformation; D017510:Protein Folding
D016326:Extracellular Matrix Proteins; D008081:Liposomes; D010455:Peptides