Detection of heparin in the salivary gland and midgut of Aedes togoi.
Mosquitoes secrete saliva that contains biological substances, including anticoagulants that counteract a host's hemostatic response and prevent blood clotting during blood feeding. This study aimed to detect heparin, an anticoagulant in Aedes togoi using an immunohistochemical detection method, in the salivary canal, salivary gland, and midgut of male and female mosquitoes. Comparisons showed that female mosquitoes contained higher concentrations of heparin than male mosquitoes. On average, the level of heparin was higher in blood-fed female mosquitoes than in non-blood-fed female mosquitoes. Heparin concentrations were higher in the midgut than in the salivary gland. This indicates presence of heparin in tissues of A. togoi.
Division of Integrative Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Young-Ran
YR
Ha
Center for Biofluid and Biomimic Research, Pohang University of Science and Technology, Pohang 790-784, Korea.
So-Ra
SR
Oh
Division of Integrative Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Eun-Seok
ES
Seo
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Bo-Heum
BH
Kim
Department of Biology, Kosin University, Busan 606-701, Korea.
Dong-Kyu
DK
Lee
Division of Integrative Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ; Center for Biofluid and Biomimic Research, Pohang University of Science and Technology, Pohang 790-784, Korea. ; School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Sang-Joon
SJ
Lee
D000330:Aedes / Q000378:metabolism*; D000818:Animals; D000925:Anticoagulants / Q000302:isolation & purification*; D001777:Blood Coagulation / Q000502:physiology; D005260:Female; D041981:Gastrointestinal Tract / Q000378:metabolism*; D006493:Heparin / Q000302:isolation & purification*; D008297:Male; D018987:Salivary Ducts / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism*
D000925:Anticoagulants; D006493:Heparin
Aedes togoi; anticoagulant; heparin; salivary gland