Fucoidan as an inhibitor of thermally induced collagen glycation examined by acetate electrophoresis.
Non-enzymatic glycation (Maillard reaction) in vitro could be a simple method to obtain glycoconjugates for studying their biological properties. Hence, fucoidan was retained by acetate electrophoresis indicating a strong interaction with the protein. A loss of colour in fucoidan bands was found for samples incubated with collagen as compared with samples of free fucoidan. Also under in vitro conditions at 100°C - simulating a sudden burn incident - fucoidan binds with collagen as a result of the Maillard reaction. In contrast, the colour of the fucoidan bands intensified for samples incubated with collagen, with the addition of glucose. Electrophoretic analyses were carried out after heating the samples to a temperature simulating a burn incident. The bands were found to intensify for samples incubated with collagen during a 30-day-long incubation. Thus, spontaneous in vitro glycation - i.e. without the addition of glucose - was confirmed. This process is highly intensified both by the temperature and time of incubation. For a sample incubated in vitro in a fucoidan solution containing glucose, glycation was confirmed in a preliminary FTIR and acetate electrophoresis examinations, occurring in collagen obtained from chicken skins. In particular, a new band emerging around 1746 cm(-1) was observed for above samples, as was its increasing intensity, as compared with samples without the addition of glucose. In the collagen glycation assay, while glucose reacts with collagen and forms cross-linked aggregates, fucoidan decreases the process of aggregation and recovery of native collagen.
Faculty of Materials and Environment Sciences, University of Bielsko-Biała, Bielsko-Biała, Poland.
Anna
A
Pielesz
Jadwiga
J
Paluch
D000085:Acetates / Q000737:chemistry*; D000818:Animals; D002645:Chickens; D003094:Collagen / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D004586:Electrophoresis / Q000379:methods*; D006031:Glycosylation / Q000187:drug effects; D015416:Maillard Reaction; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D012867:Skin / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D000085:Acetates; D011134:Polysaccharides; D003094:Collagen; C007789:fucoidan
Acetate electrophoresis; Collagen from organic chicken skin; Non-enzymatic glycation; Partially hydrolysed fucoidan