Relationship between SDF-1G801A polymorphism and its expression in Tunisian patients with colorectal cancer.
The aim was to evaluate the relationship between SDF-1G801A polymorphism and its immunohistochemical expression in colorectal cancer tissues in the Tunisian cohort. The molecular and immunohistochemical analysis showed that SDF-1G801A polymorphic variant was higher in CRC patients with TNM stage II and III, the SDF-1 expression was significantly increased from normal mucosa to primary tumor (p < 0.05). CRC patients have higher frequency of A allele (52.01%) than controls (26.8%) (P = 0.0001). Thus, SDF-1 polymorphism is a risk factor of colorectal cancer susceptibility in our population, the polymorph genotype of SDF-1 maybe associated with clinical manifestations in CRC patients in Tunisia.
a Laboratory of Colorectal Cancer Research UR03ES04 , Tunis , Tunisia.
Sameh
S
Amara
Ines
I
Chaar
Meriem
M
Khiari
Donia
D
Ounissi
Abdelmajid B
AB
Hmida
Sabeh Regaya
SR
Mzabi
Saadia
S
Bouraoui
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D054377:Chemokine CXCL12 / Q000235:genetics* / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D014416:Tunisia; D055815:Young Adult
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12
PCR-RFLP; SDF-1 SNP (single nucleotide polymorphism); SDF-1 expression; colorectal cancer; immunohistochemestry