Evaluation of oxidative stress in the late postoperative stage of liver transplantation.
BACKGROUND
Liver transplant recipients are at an increased oxidative stress risk due to pre-existing hepatic impairment, ischemia-reperfusion injury, immunosuppression, and functional graft rejection. This study compared the oxidative status of healthy control subjects, patients with liver cirrhosis on the list for transplantation, and subjects already transplanted for at least 12 months.


METHODS
Sixty adult male patients, aged between 27 and 67 years, were subdivided into 3 groups: a control group (15 healthy volunteers), a cirrhosis group (15 volunteers), and a transplant group (30 volunteers). Oxidative stress was evaluated by activity of reduced glutathione, malondialdehyde, and vitamin E.


RESULTS
There was a significant difference (P < .01) in the plasma concentration of reduced glutathione in the 3 groups, with the lowest values observed in the transplanted group. The malondialdehyde values differed significantly (P < .01) among the 3 groups, with the transplanted group again having the lowest concentrations. The lowest concentrations of vitamin E were observed in patients with cirrhosis compared with control subjects, and there was a significant correlation (P < .05) among the 3 groups. No correlations were found between reduced glutathione and vitamin E or between vitamin E and malondialdehyde. However, there were strong correlations between plasma malondialdehyde and reduced glutathione in the 3 groups: control group, r = 0.9972 and P < .0001; cirrhotic group, r = 0.9765 and P < .0001; and transplanted group, r = 0.8981 and P < .0001.


CONCLUSIONS
In the late postoperative stage of liver transplantation, oxidative stress persists but in attenuated form.
Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo, SP, Brazil.
V S
VS
Augusto
Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo, SP, Brazil.
A J
AJ
Rodrigues
Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo, SP, Brazil.
G S
GS
Reis
Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo, SP, Brazil.
A P C
AP
Silveira
Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo, SP, Brazil.
O
O
de Castro e Silva
Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo, SP, Brazil.
E D
ED
Mente
Department of Internal Medicine, Ribeirão Preto Faculty of Medicine, University of São Paulo, SP, Brazil.
A A
AA
Jordão
Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo, SP, Brazil. Electronic address: prbevora@gmail.com.
P R B
PR
Evora
D000328:Adult; D016022:Case-Control Studies; D005978:Glutathione / Q000097:blood; D006801:Humans; D007166:Immunosuppressive Agents / Q000008:administration & dosage; D016031:Liver Transplantation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D011184:Postoperative Period
D007166:Immunosuppressive Agents; D008315:Malondialdehyde; D005978:Glutathione