Novel devices for solvent delivery and temperature programming designed for capillary liquid chromatography.
Analyses in chromatographic systems able to save mobile and stationary phases without reducing efficiency and resolution are of current interest. These advantages regarding savings have challenged us to develop a system dedicated to miniaturized liquid chromatography. This paper reports on the development of a high-pressure syringe-type pump, an oven able to perform isothermal and temperature programming and a software program to control these chromatographic devices. The experimental results show that the miniaturized system can generate reproducible and accurate temperature and flow rate. The system was applied to the separation of statins and tetracylines and showed excellent performance.
Institute of Chemistry of Sao Carlos, University of Sao Paulo, Sao Carlos (SP), Brazil.
Lincoln Figueira Marins
LF
Coutinho
Carlos Eduardo Domingues
CE
Nazario
Alessandra Maffei
AM
Monteiro
Fernando Mauro
FM
Lanças
D000596:Amino Acids / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D013696:Temperature; D013752:Tetracycline / Q000302:isolation & purification*
D000596:Amino Acids; D013752:Tetracycline; C013165:statine
Instrumentation; Miniaturization; Oven; Pump; Temperature programming