Simiduia litorea sp. nov., isolated from seashore sediments of the Sea of Japan.
An aerobic, Gram-stain-negative, agarolytic rod-shaped bacterium, designated KMM 9504(T), was isolated from a sediment sample collected from the seashore of the Sea of Japan. Comparative 16S rRNA gene sequence analysis showed that strain KMM 9504(T) belonged to the genus Simiduia as it was most closely related to Simiduia areninigrae KCTC 23293(T) (97.3% sequence similarity). Strain KMM 9504(T) was characterized by the major ubiquinone Q-8, and by the predominance of C(16 : 1)ω7c, C(17 : 1)ω8c, followed by C(16 : 0), C(15 : 0), C(17 : 0) and C(12 : 1) in its fatty acid profile. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminophospholipid, an unknown aminolipid, unknown phospholipids, and unknown lipids. Based on the distinctive phenotypic characteristics, phylogenetic analysis and DNA-DNA hybridization results, it is concluded that strain KMM 9504(T) represents a novel species of the genus Simiduia, for which the name Simiduia litorea sp. nov. is proposed. The type strain of the species is strain KMM 9504(T) ( = NRIC 0917(T) = JCM 19759(T)).
NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Naoto
N
Tanaka
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok 690022, Russia.
Lyudmila A
LA
Romanenko
Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690041, Russia.
Vassilii I
VI
Svetashev
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok 690022, Russia.
Valery V
VV
Mikhailov
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8