The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus.
The impact of in vivo and in vitro exposure to anticancer drug 5-Fluorouracil (5-FU) on the level of DNA damage was evaluated using comet assay on haemocytes of freshwater mussels Unio pictorum and Unio tumidus. For the in vivo experiment, the groups of 5 mussels per concentration were exposed for 72 h to 5-FU (0.04, 0.4, 4, 40 and 100 μM) with 0.4 μM being the lowest concentration to induce significant DNA damage. For the in vitro experiment, the primary cultures of haemocytes were treated with 0.04, 0.4, 4 and 40 μM 5-FU for 22 h and the treatment with CdCl2 was used as a positive control. In contrast to in vivo exposure, 5-FU did not induce significant increase of DNA damage in vitro, possibly because of the absence of haemocytes proliferation in primary cultures.
University of Belgrade, Institute for Multidisciplinary Research, Kneza Višeslava 1, 11000 Belgrade, Serbia. Electronic address: zorga@imsi.rs.
Zoran
Z
Gačić
University of Belgrade, Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Studentski trg 16, 11000 Belgrade, Serbia. Electronic address: stoimirk@bio.bg.ac.rs.
Stoimir
S
Kolarević
University of Belgrade, Institute for Multidisciplinary Research, Kneza Višeslava 1, 11000 Belgrade, Serbia.
Karolina
K
Sunjog
University of Belgrade, Institute for Biological ResearchSiniša Stanković, Despota Stefana 142, 11000 Belgrade, Serbia.
Margareta
M
Kračun-Kolarević
University of Belgrade, Institute for Biological ResearchSiniša Stanković, Despota Stefana 142, 11000 Belgrade, Serbia. Electronic address: mpaunovi@ibiss.ac.rs.
Momir
M
Paunović
University of Belgrade, Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Studentski trg 16, 11000 Belgrade, Serbia.
Jelena
J
Knežević-Vukčević
University of Belgrade, Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Studentski trg 16, 11000 Belgrade, Serbia. Electronic address: brankavg@bio.bg.ac.rs.
Branka
B
Vuković-Gačić
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000633:toxicity; D049872:Bivalvia / Q000235:genetics; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005472:Fluorouracil / Q000633:toxicity*; D005618:Fresh Water; D006434:Hemocytes / Q000187:drug effects; D049881:Unio / Q000187:drug effects / Q000235:genetics
D000964:Antimetabolites, Antineoplastic; D005472:Fluorouracil
5-Fluorouracil; Comet assay; Unio pictorum; Unio tumidus