Aminophenylboronic-acid-conjugated polyacrylic acid-Mn-doped ZnS quantum dot for highly sensitive discrimination of glycoproteins.
Discrimination of glycoproteins with different glycans is a significant but difficult issue. We presented here a new strategy for strengthening the discrimination of glycoproteins by introducing a new signaling channel, fluorescence polarization (FP), into a "single probe with three signaling channels" sensor array. The single probe was aminophenylboronic-acid-conjugated poly(acrylic acid)-Mn-doped ZnS quantum dots, and the three signaling channels were FP, room temperature phosphorescence and light scattering. Ten glycoproteins, including ovalbumin, fibrinogen, transferrin, horseradish peroxidase, vascular endothelial growth factor, immunoglobulin G, avidin, hyaluronidase, cellulase R-10, and glucose oxidase, were involved for evaluating the discriminating capability. The introduction of the FP signaling channel improved the discriminating power of the sensor array, so that the 10 glycoproteins at 0.15 μM could be well discriminated both in PBS buffer and in the presence of human serum sample. The identification accuracy of the unknown samples was 96.25% (77 out of 80) at the 0.15 μM level and 97.50% (78 out of 80) at the 0.2 μM level. The integration of the signaling patterns with different responsive principles was demonstrated as the promising way to enhance the discrimination power of the single-probe-based sensor arrays.
State Key Laboratory of Medicinal Chemical Biology, and Research Center for Analytical Sciences, College of Chemistry, Nankai University , Tianjin 300071, China.
Li-Jia
LJ
Sang
He-Fang
HF
Wang
D000180:Acrylic Resins / Q000737:chemistry*; D001897:Boronic Acids / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis*; D008345:Manganese / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots; D017550:Spectroscopy, Fourier Transform Infrared; D013440:Sulfides / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
D000180:Acrylic Resins; D001897:Boronic Acids; D006023:Glycoproteins; D013440:Sulfides; D017967:Zinc Compounds; C028592:3-aminobenzeneboronic acid; D008345:Manganese; C006903:carbopol 940; C031238:zinc sulfide