Clinical application and evaluation of Sysmex XE-5000 analyser for detecting nucleated red blood cells in peripheral blood.
BACKGROUND
To evaluate the performance of Sysmex XE-5000 analyser for detecting nucleated red blood cells (NRBCs) in peripheral blood and investigate the clinical application of this analyser.


METHODS
The absolute NRBC counts (NRBC#) and percentage (NRBC%) of 137 blood specimens (NRBC-positive according to the DIFF channel of the analyser) were determined in the NRBC channel of the analyser. The intra-assay imprecision, carryover rate, and linear range of the analyser were evaluated. The NRBC% of the blood sample was detected with a microscope, and the difference between two methods was analysed.


RESULTS
The intra-assay imprecision of the analyser for detecting NRBC# in specimens with high, moderate, and low Q-flag values were 2.10%, 3.26%, and 11.62%, respectively, and the imprecision for detecting NRBC% were 3.79%, 5.80%, and 13.33%, respectively. The carryover rates of the analyser for detecting NRBC# and NRBC% were 0.51% and 0.26%, respectively.


CONCLUSIONS
Sysmex XE-5000 analyser had good linearity in NRBC# (i.e., 0/L to 18 x 10(9)/L). The NRBC%s of the two methods did not significantly differ (p = 0.716). The analyser can completely replace the traditional microscope for clinically classifying and counting NRBCs.
Shan-Luan
SL
Zheng
Tian
T
Zheng
Xiang
X
Cheng
Xiao-Ke
XK
Hao
D002454:Cell Differentiation / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics / Q000473:pathology; D004900:Erythroblasts / Q000145:classification / Q000166:cytology / Q000473:pathology; D004906:Erythrocyte Count / Q000295:instrumentation*; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D008853:Microscopy