Measurement of T₁/T₂ relaxation times in overlapped regions from homodecoupled ¹H singlet signals.
The implementation of the HOmodecoupled Band-Selective (HOBS) technique in the conventional Inversion-Recovery and CPMG-based PROJECT experiments is described. The achievement of fully homodecoupled signals allows the distinction of overlapped (1)H resonances with small chemical shift differences. It is shown that the corresponding T1 and T2 relaxation times can be individually measured from the resulting singlet lines using conventional exponential curve-fitting methods.
Servei de Ressonància Magnètica Nuclear and Departament de Química, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Catalonia, Spain.
Laura
L
Castañar
Servei de Ressonància Magnètica Nuclear and Departament de Química, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Catalonia, Spain.
Pau
P
Nolis
Servei de Ressonància Magnètica Nuclear and Departament de Química, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Catalonia, Spain.
Albert
A
Virgili
Servei de Ressonància Magnètica Nuclear and Departament de Química, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Catalonia, Spain. Electronic address: teodor.parella@uab.cat.
Teodor
T
Parella
D000465:Algorithms; D001704:Biopolymers / Q000032:analysis* / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009716:Numerical Analysis, Computer-Assisted; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001704:Biopolymers
CPMG; HOBS; Inversion-Recovery; PROJECT; Pure-shift NMR; Relaxation times; T(1); T(2)