IncI1 plasmids carrying bla(CTX-M-1) or bla(CMY-2) genes in Escherichia coli from healthy humans and animals in Tunisia.
The objective was to determine the location of bla(CTX-M-1) and bla(CMY-2) genes in 33 Escherichia coli isolates previously obtained from healthy humans, pets, and food-producing animals in Tunisia, and to characterize the genetic lineages of isolates. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE)-XbaI and multilocus sequence typing (MLST). Plasmids were analyzed by S1-PFGE, polymerase chain reaction-based replicon typing, and plasmid MLST. Conjugation experiments were performed. The bla(CTX-M-1) and bla(CMY-2) genes were studied by I-Ceu1-PFGE and S1-PFGE, and subsequent hybridization with specific probes. Eighteen different sequence types (STs) were identified among the 30 CTX-M-1-producing isolates, 5 of them being detected in 17 isolates (ST/phylogroup): ST57/D, ST155/B1, ST58/B1, ST10/A, and ST398/A. Most of the bla(CTX-M-1)-positive isolates had different PFGE profiles, with the exception of four human and pet isolates of lineage ST57 with related PFGE profiles (>80% identity). Three CMY-2-producing isolates were typed as ST58/B1, ST117/D, and ST3632/B2. The IncI1 replicon was detected in all the 33 E. coli studied isolates, in many cases in combination with other replicons: IncF, IncX, IncK, IncR, IncY, colE, or IncN. The bla(CTX-M-1) gene was transferred by conjugation in 22 of the 30 positive strains and was located into IncI1 plasmids (ST3-CC3); the bla(CMY-2) gene was located into a conjugative IncI1 plasmid (ST12) of 97 kb in one strain. One bla(CTX-M-1)-positive strain carried the qnrB19 gene in a 33 kb IncX plasmid. Diverse genetic lineages are detected in extended-spectrum beta-lactamase- and AmpC beta-lactamase-producing E. coli from different origins. The bla(CTX-M-1) and bla(CMY-2) genes were associated with conjugative IncI1 (ST3 and ST12, respectively) plasmids in E. coli strains from human and animal origin.
1 Laboratoire Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis-El Manar , Tunis, Tunisia .
Rym
R
Ben Sallem
Karim
K
Ben Slama
Beatriz
B
Rojo-Bezares
Nerea
N
Porres-Osante
Ahlem
A
Jouini
Naouel
N
Klibi
Abdellatif
A
Boudabous
Yolanda
Y
Sáenz
Carmen
C
Torres
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D002353:Carrier State; D002415:Cats; D002645:Chickens; D003227:Conjugation, Genetic; D004285:Dogs; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology; D015870:Gene Expression; D006801:Humans; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D057805:Pets / Q000382:microbiology; D010957:Plasmids; D014416:Tunisia / Q000453:epidemiology; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; C103022:beta-lactamase CMY-2; C055184:beta-lactamase TEM-3; D001618:beta-Lactamases