[Ultra-high performance liquid chromatography-mass spectrometry for analysis of newborn and fetal bovine serum components].
We used ultra-high performance liquid chromatography-mass spectrometry (UPLC/MS) for analyzing and identifying the active components of newborn calf serum (NCS) and fetal bovine serum (FBS). The results demonstrated significant differences in the components between NCS and FBS. FBS appeared to have more complex components than NCS, with mass to ratios (m/z) of the substances of 498, 273 and 448. These substances in FBS may be the main active components to support the proliferation and differentiation of cells.
D Kunming Medical University, Kunming 650500, China. E-mail: licaixia1023@163.com.
Caixia
C
Li
Fuke
F
Wang
Liu
L
Liu
D000818:Animals; D000831:Animals, Newborn / Q000097:blood; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005312:Fetal Blood / Q000737:chemistry; D013058:Mass Spectrometry; D044967:Serum / Q000737:chemistry*