Dendritic planarity of Purkinje cells is independent of Reelin signaling.
The dendritic planarity of Purkinje cells is critical for cerebellar circuit formation. In the absence of Crk and CrkL, the Reelin pathway does not function resulting in partial Purkinje cell migration and defective dendritogenesis. However, the relationships among Purkinje cell migration, dendritic development and Reelin signaling have not been clearly delineated. Here, we use synchrotron X-ray microscopy to obtain 3-D images of Golgi-stained Purkinje cell dendrites. Purkinje cells that failed to migrate completely exhibited conical dendrites with abnormal 3-D arborization and reduced dendritic complexity. Furthermore, their spines were fewer in number with a distorted morphology. In contrast, Purkinje cells that migrated successfully displayed planar dendritic and spine morphologies similar to normal cells, despite reduced dendritic complexity. These results indicate that, during cerebellar formation, Purkinje cells migrate into an environment that supports development of dendritic planarity and spine formation. While Reelin signaling is important for the migration process, it does not make a direct major contribution to dendrite formation.
X-ray Imaging Center, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.
Jinkyung
J
Kim
Tae-Ju
TJ
Park
Namseop
N
Kwon
Dongmyeong
D
Lee
Seunghwan
S
Kim
Yoshiki
Y
Kohmura
Tetsuya
T
Ishikawa
Kyong-Tai
KT
Kim
Tom
T
Curran
Jung Ho
JH
Je
D048868:Adaptor Proteins, Signal Transducing / Q000172:deficiency / Q000235:genetics; D000818:Animals; D001931:Brain Mapping; D064026:Calbindins / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D002465:Cell Movement / Q000235:genetics; D002531:Cerebellum / Q000166:cytology*; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics; D021621:Imaging, Three-Dimensional; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000172:deficiency / Q000235:genetics; D050737:Proto-Oncogene Proteins c-crk / Q000172:deficiency / Q000235:genetics; D011689:Purkinje Cells / Q000378:metabolism* / Q000648:ultrastructure*; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D016622:Silver Staining; D014057:Tomography, X-Ray Computed
D048868:Adaptor Proteins, Signal Transducing; C082869:CRKL protein; D064026:Calbindins; D015816:Cell Adhesion Molecules, Neuronal; C476798:Crk protein, mouse; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D050737:Proto-Oncogene Proteins c-crk; D000091042:Reelin Protein; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases