Multi-residue and multi-class method for the determination of antibiotics in bovine muscle by ultra-high-performance liquid chromatography tandem mass spectrometry.
A multi-residue quantitative screening method covering 41 antibiotics from 7 different families, by ultra-high-performance-liquid-chromatography tandem mass spectrometry (UHPLC-MS/MS), is described. Sulfonamides, trimethoprim, tetracyclines, macrolides, quinolones, penicillins and chloramphenicol are simultaneously detected after a simple sample preparation of bovine muscle optimized to achieve the best recovery for all compounds. A simple sample treatment was developed consisting in an extraction with a mixture of acetonitrile and ethylenediaminetetraacetic acid (EDTA), followed by a defatting step with n-hexane. The methodology was validated, in accordance with Decision 2002/657/EC by evaluating the required parameters: decision limit (CCα), detection capability (CCβ), specificity, repeatability and reproducibility. Precision in terms of relative standard deviation was under 20% for all compounds and the recoveries between 91% and 119%. CCα and CCβ were determined according the maximum residue limit (MRL) or the minimum required performance limit (MRPL), when required.
INIAV-LNIV, Laboratório Nacional de Investigação Veterinária, Estrada de Benfica, 701, 1549-011 Lisboa, Portugal.
Andreia
A
Freitas
INIAV-LNIV, Laboratório Nacional de Investigação Veterinária, Estrada de Benfica, 701, 1549-011 Lisboa, Portugal.
Jorge
J
Barbosa
CNC - Centro de Neurociências e Biologia Celular, Pólo das Ciências da Saúde, Faculdade de Farmácia, Universidade de Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal. Electronic address: fjramos@ci.uc.pt.
Fernando
F
Ramos
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D004492:Edetic Acid; D005504:Food Analysis / Q000379:methods*; D006586:Hexanes; D018942:Macrolides / Q000032:analysis; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D010406:Penicillins / Q000032:analysis; D015363:Quinolones / Q000032:analysis; D015203:Reproducibility of Results; D013449:Sulfonamides / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013754:Tetracyclines / Q000032:analysis; D014295:Trimethoprim / Q000032:analysis
D000900:Anti-Bacterial Agents; D006586:Hexanes; D018942:Macrolides; D010406:Penicillins; D015363:Quinolones; D013449:Sulfonamides; D013754:Tetracyclines; C026385:n-hexane; D004492:Edetic Acid; D014295:Trimethoprim
Antibiotics; Multi-class; Multi-detection; Muscle; UHPLC–MS/MS; Validation