Identification of proteins associated with lymph node metastasis of gastric cancer.
OBJECTIVE
The aim of this study was to identify proteins associated with gastric cancer lymph node metastasis and explore the clinicopathological significance of these proteins.


METHODS
Gastric cancer tissues were obtained from 24 patients with high or low lymph node metastatic potential. Total cellular proteins were separated by two-dimensional gel electrophoresis (2-DE), analyzed by MALDI/TOF-TOF MS, and identified by a database search. Expression of 14-3-3β and profilin-1 was then immunohistochemically verified in paraffin-embedded gastric cancer tissues from 128 patients and analyzed by multivariate logistic regression models, Kaplan-Meier curves, and Cox proportional hazard models.


RESULTS
A total of 26 differentially expressed proteins were identified, 20 of which were overexpressed and 6 of which were underexpressed. 14-3-3β and profilin-1 were upregulated in gastric cancer tissues with and without lymph node metastasis, respectively. Expression of 14-3-3β protein was associated, but profilin-1 expression was inversely associated with gastric cancer lymph node metastasis. Multivariate analysis showed that overexpression of 14-3-3β and reduced expression of profilin-1 were independent risk factors for gastric cancer lymph node metastasis, while 14-3-3β overexpression was an independent prognostic factor for gastric cancer patients.


CONCLUSIONS
The current study identified 26 differentially expressed proteins. Further studies showed that both 14-3-3β and profilin-1 protein may be useful biomarkers for prediction of gastric cancer lymph node metastasis and that expression of 14-3-3β was a prognostic marker for gastric cancer patients.
Department of Gastroenterology, Tumor Hospital of Harbin Medical University, 150 Haping Road, Harbin, 150086, Helongjiang, China.
Yan
Y
Ma
Yan-Feng
YF
Li
Tie
T
Wang
Rui
R
Pang
Ying-Wei
YW
Xue
Shu-Peng
SP
Zhao
D048948:14-3-3 Proteins / Q000032:analysis*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005743:Gastrectomy; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008198:Lymph Nodes / Q000737:chemistry / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D051304:Profilins / Q000032:analysis*; D011379:Prognosis; D016016:Proportional Hazards Models; D011446:Prospective Studies; D012189:Retrospective Studies; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000473:pathology* / Q000601:surgery; D015854:Up-Regulation
D048948:14-3-3 Proteins; D014408:Biomarkers, Tumor; D051304:Profilins