Poly(L-lactide-co-glycolide) thin films can act as autologous cell carriers for skin tissue engineering.
Degradable aliphatic polyesters such as polylactides, polyglycolides and their copolymers are used in several biomedical and pharmaceutical applications. We analyzed the influence of poly(L-lactide-co-glycolide) (PLGA) thin films on the adhesion, proliferation, motility and differentiation of primary human skin keratinocytes and fibroblasts in the context of their potential use as cell carriers for skin tissue engineering. We did not observe visible differences in the morphology, focal contact appearance, or actin cytoskeleton organization of skin cells cultured on PLGA films compared to those cultured under control conditions. Moreover, we did not detect biologically significant differences in proliferative activity, migration parameters, level of differentiation, or expression of vinculin when the cells were cultured on PLGA films and tissue culture polystyrene. Our results indicate that PLGA films do not affect the basic functions of primary human skin keratinocytes and fibroblasts and thus show acceptable biocompatibility in vitro, paving the way for their use as biomaterials for skin tissue engineering.
Laboratory of Cell & Tissue Engineering, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology and Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, 30-387, Poland.
Aleksandra
A
Zuber
Julia
J
Borowczyk
Eliza
E
Zimolag
Malgorzata
M
Krok
Zbigniew
Z
Madeja
Elzbieta
E
Pamula
Justyna
J
Drukala
D008841:Actin Cytoskeleton / Q000187:drug effects; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D011100:Polyglycolic Acid / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012038:Regeneration / Q000187:drug effects; D012867:Skin / Q000378:metabolism; D013499:Surface Properties; D023822:Tissue Engineering; D016596:Vinculin / Q000378:metabolism
D001672:Biocompatible Materials; D016596:Vinculin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid