Occupation exposures and sperm morphology: a case-referent analysis of a multi-centre study.
OBJECTIVE
We examined occupational exposures and sperm morphology to establish whether exposures implicated differed from those affecting motile sperm concentration.


METHODS
Computer aided sperm morphometric assessment was undertaken on morphology slides obtained as part of a multi-centre study in 1999-2002 of occupational factors in male infertility. Men attending 14 fertility clinics across the UK were recruited and gave a semen sample. Before results of the semen analysis were known, the men completed detailed questionnaires about their employment and lifestyle. Occupational exposures were assessed by occupational hygienists. Data were analysed using an unmatched case-referent design, allowing for clustering and for confounders. Three case definitions were used: poor morphology (normal morphology <4%), low motile sperm count (MSC) (<4.8×10(6)) and either condition.


RESULTS
Morphology results were available for 1861/2011 men employed at the time of recruitment. Of these 1861, 296 (15.9%) had poor morphology; of the 2011with sperm count, 453 (22.5%) had low MSC; 654/1981 (33.0%) had either condition. Poor morphology, adjusted for confounding, was related to self-reported lifetime exposure to lead (OR=1.33; 95% CI 1.00 to 1.75). Low MSC was also related to self-reported lead and to hygienist-assessed glycol ether exposure. Self-reported use of paint stripper (OR=1.47; 95% CI 1.07 to 2.03) and lead, but not glycol ether, were significantly related to the combined case definition.


CONCLUSIONS
While this study did not identify any occupational exposure uniquely related to sperm morphology, the capacity of the study to detect risk was increased by including morphology with sperm concentration and motility.
Division of Preventive Medicine, University of Alberta, Edmonton, Alberta, Canada.
Nicola
N
Cherry
School of Community-based Medicine, University of Manchester, Manchester, UK.
Andy C
AC
Povey
School of Community-based Medicine, University of Manchester, Manchester, UK.
Roseanne
R
McNamee
Reproductive and Developmental Medicine, University of Sheffield, Sheffield, UK.
Harry
H
Moore
Reproductive and Developmental Medicine, University of Sheffield, Sheffield, UK.
Helen
H
Baillie
School of Community-based Medicine, University of Manchester, Manchester, UK.
Julie-Ann
JA
Clyma
School of Community-based Medicine, University of Manchester, Manchester, UK.
Martin
M
Dippnall
Reproductive and Developmental Medicine, University of Sheffield, Sheffield, UK.
Allan A
AA
Pacey
D000328:Adult; D016022:Case-Control Studies; D006018:Glycols / Q000633:toxicity*; D006801:Humans; D007854:Lead / Q000633:toxicity*; D008019:Life Style; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects*; D055101:Semen Analysis; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities*; D011795:Surveys and Questionnaires; D006113:United Kingdom
D006018:Glycols; D007854:Lead