Therapeutic microbes for infectious disease.
The rapid emergence of multidrug-resistant pathogens has invoked concerns of our current limitations in controlling the spread of infectious disease. To resolve this, we have applied synthetic biology principles to engineer human commensal microbe that can specifically sense and kill an antibiotic-resistant strain of P. aeruginosa. In this chapter, we describe the methods used to assemble, characterize, and evaluate the effectiveness of our engineered microbe in multicellular systems.
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore.
Choon Kit
CK
Wong
Mui Hua
MH
Tan
Bahareh Haji
BH
Rasouliha
In Young
IY
Hwang
Hua
H
Ling
Chueh Loo
CL
Poh
Matthew Wook
MW
Chang
D018920:Coculture Techniques / Q000379:methods; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology* / Q000628:therapy*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology*; D053038:Quorum Sensing; D058615:Synthetic Biology / Q000379:methods