Molecular characterization of the hippeastrum chlorotic ringspot virus L segment and its protein.
Hippeastrum chlorotic ringspot virus (HCRV) is a novel tospovirus that was identified in Yunnan Province, China, in 2013. We have sequenced the HCRV L gene, which is 8909 nt long and encodes the RNA-dependent RNA polymerase (2873 amino acids, 330.8 kDa). The HCRV L protein shared highest similarity (89.4 %) with that of tomato yellow ring virus. The L protein contains a negative-sense RNA virus RNA-directed RNA polymerase motif and an endonuclease domain at the N-terminus. Combined with our previous reports of the S and M RNAs, the genome sequence of HCRV is now completed.
College of plant protection, Yunnan Agricultural University, Kunming, 650201, China.
Ye
Y
Xu
Shi-Gao
SG
Lou
Qiu-Fang
QF
Li
Qing
Q
Hu
Li-Peng
LP
Sun
Zheng-Yue
ZY
Li
Yong-Zhong
YZ
Li
Ya-Ting
YT
Liu
D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D014644:Genetic Variation; D027762:Lilium / Q000821:virology*; D016366:Open Reading Frames / Q000235:genetics; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D017865:Tospovirus / Q000201:enzymology* / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase