Enhancing proliferation and ECM expression of human ACL fibroblasts by sonic vibration.
Effects of mechanical vibration on cell activity and behavior remain controversial: There has been evidence on both positive and negative effects. Furthermore, research on the anterior cruciate ligament (ACL) has as yet been limited and the frequency-related effects remain unknown, even though ACL injury is common and an injured ACL hardly spontaneously recovers. The object of this work was to address the influence of mechanical vibration on ACL fibroblasts, to determine the effects of frequencies, and to further study this effect at the cellular level. We found that sonic vibration affected ACL fibroblasts' proliferation and metabolism in a frequency-dependent manner, and 20 Hz gave rise to the most ACL cell activity and comprehensively increased extracellular matrix (ECM) contents, including collagen type I, collagen type III, fibronectin, elastin, tenascin, glycosaminoglycan (GAG), and the cytoskeleton protein vimentin. Thus, our results indicate that sonic vibration possesses frequency-dependent effects on proliferation and productivity of ACL fibroblast with an optimal frequency of 20 Hz under the present stimulation conditions, providing further information for future research in how vibrational stimulation manipulates ACL cellular behavior.
a Department of Medical Biotechnology , Dongguk University , Seoul , Korea.
Yuan-Yuan
YY
Jiang
Jung-Keug
JK
Park
Hee-Hoon
HH
Yoon
Hynjin
H
Choi
Chan-Wha
CW
Kim
Young-Kwon
YK
Seo
D000328:Adult; D016118:Anterior Cruciate Ligament / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D004549:Elastin / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D013010:Sonication; D019063:Tenascin / Q000378:metabolism; D014732:Vibration; D014746:Vimentin / Q000378:metabolism
D024042:Collagen Type I; D024061:Collagen Type III; D005353:Fibronectins; D006025:Glycosaminoglycans; D019063:Tenascin; D014746:Vimentin; D004549:Elastin
extracellular matrix; human anterior cruciate ligament fibroblast; proliferation; sonic vibration