Herbal Prescription, DSGOST, Prevents Cold-Induced RhoA Activation and Endothelin-1 Production in Endothelial Cells.
Herbal prescription, Danggui-Sayuk-Ga-Osuyu-Saenggang-tang (DSGOST), has long been used to treat Raynaud's phenomenon (RP) in traditional Chinese medicine (TCM). However, a biological mechanism by which DSGOST ameliorates RP is yet deciphered. In this study, we demonstrate that DSGOST inhibits cold-induced activation of RhoA, in both vascular smooth muscle cells (VSMC) and endothelial cells (EC), and blocks endothelin-1-mediated paracrine path for cold response on vessels. While cold induced RhoA activity in both cell types, DSGOST pretreatment prevented cold-induced RhoA activation. DSGOST inhibition of cold-induced RhoA activation further blocked α 2c-adrenoreceptor translocation to the plasma membrane in VSMC. In addition, DSGOST inhibited endothelin-1-mediated RhoA activation and α 2c-adrenoreceptor translocation in VSMC. Meanwhile, DSGOST inhibited cold-induced or RhoA-dependent phosphorylation of FAK, SRC, and ERK. Consistently, DSGOST inhibited cold-induced endothelin-1 expression in EC. Therefore, DSGOST prevents cold-induced RhoA in EC and blocks endothelin-1-mediated paracrine path between EC and VSMC. In conclusion, our data suggest that DSGOST is beneficial for treating RP-like syndrome.
Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, Seoul 130701, Republic of Korea.
Sung-Gook
SG
Cho
Department of Korean Internal Medicine, College of Korean Medicine, Semyung University, Chungju 380960, Republic of Korea.
Ho Yeon
HY
Go
Department of Korean Internal Medicine, College of Korean Medicine, Gachon University, Seongnam 461701, Republic of Korea.
Jeong-Su
JS
Park
Department of Korean Internal Medicine, College of Korean Medicine, Gachon University, Seongnam 461701, Republic of Korea.
Ki-Yong
KY
Jung
Department of Oriental Internal Medicine, College of Korean Medicine, Sangji University, Wonju 220702, Republic of Korea.
Seung-Ho
SH
Sun
Department of Korean Internal Medicine, College of Korean Medicine, Gachon University, Seongnam 461701, Republic of Korea.
You-Kyung
YK
Choi
Department of Korean Rehabilitation Medicine, College of Korean Medicine, Gachon University, Seongnam 461701, Republic of Korea.
Yun-Kyung
YK
Song
Department of Korean Internal Medicine, College of Korean Medicine, Gachon University, Seongnam 461701, Republic of Korea.
Jong-Hyeong
JH
Park
Department of Korean Internal Medicine, College of Korean Medicine, Gachon University, Seongnam 461701, Republic of Korea.
Chan-Yong
CY
Jun
Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, Seoul 130701, Republic of Korea.
Seong Gyu
SG
Ko