Application of maltodextrin as chiral selector in capillary electrophoresis for quantification of amlodipine enantiomers in commercial tablets.
Maltodextrin was investigated as a chiral selector in capillary electrophoresis (CE) analysis of amlodipine (AM) enantiomers. For development of a stereoselective CE method, various effective parameters on the enantioseparation were optimized. The best results were achieved on an uncoated fused silica capillary at 20 °C using phosphate buffer (100 mM, pH 4) containing 10% w/v maltodextrin (dextrose equivalent value 4-7). The UV detector was set at 214 nm and a constant voltage of 20 kV was applied. The range of quantitation was 2.5-250 µg/mL (R(2)  > 0.999) for both enantiomers. Intra- (n = 5) and interday (n = 3) relative standard deviation (RSD) values were less than 7%. The limits of quantitation and detection were 1.7 µg/mL and 0.52 µg/mL, respectively. Recoveries of R(+) and S(-) enantiomers from tablet matrix were 97.2% and 97.8%, respectively. The method was applied for the quantification of AM enantiomers in commercial tablets. Also, the enantioseparation capability of heparin was evaluated and the results showed that heparin did not have any chiral selector activity in this study.
Faculty of Chemistry, Shahid Beheshti University, Evin, Tehran, Iran.
Saeed
S
Nojavan
Shabnam
S
Pourmoslemi
Hamideh
H
Behdad
Ali Reza
AR
Fakhari
Ali
A
Mohammadi
D017311:Amlodipine / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000191:economics / Q000379:methods*; D005947:Glucose / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000737:chemistry*; D013237:Stereoisomerism; D013607:Tablets
D004573:Electrolytes; D011134:Polysaccharides; D013607:Tablets; D017311:Amlodipine; C008315:maltodextrin; D005947:Glucose
amlodipine; capillary electrophoresis; enantioseparation; heparin; maltodextrin