Thrombin generation increasing with age and decreasing with use of heparin indicated by calibrated automated thrombogram conducted in Chinese.
OBJECTIVE
Calibrated Automated Thrombogram(CAT) is a test to monitor the generation of thrombin. It can be described by four parameters: lag time, peak thrombin, endogenous thrombin potential (ETP) and time to peak (ttPeak). This study aims to determine the normal ranges of CAT parameters in Chinese, and evaluate whether thrombin generation is correlated with the concentration of heparin/low molecular weight heparin.


METHODS
Plasma from 120 healthy subjects were collected to determine the normal rangea of CAT parameters in Chinese. Normal plasma pool (NPP, n=25) spiked with different concentrations of heparin or enoxaparin were used to detecte CAT parameters. The overall and age specific normal ranges of CAT parameters were calculated using descriptive statistics method with mean±2SD. The correlation between CAT parameters and age or concentrations of heparin, enoxaparin were analyzed with linear regression model.


RESULTS
The normal ranges for lag time, peak thrombin, ETP, ttPeak in the subjects were 3.648±2.465 min, 367.39±151.93 nmol/L, 2277±1030 nmol/L•min and 6.372±4.280 min respectively. Age was linearly correlated with lag time (r=-0.6583, P<0.0001), peak thrombin (r=0.4863, P<0.0001), ETP (r=0.3608, P<0.0014) and ttPeak (r=-0.6313, P<0.0001). The values of ETP/peak ratio were linearly correlated with concentrations of heparin.


CONCLUSIONS
The normal ranges of four CAT parameters for Chinese were determined. CAT parameters are associated with age. ETP/peak ratio could be used to monitor the process of anticoagulation therapy.
Department of laboratory medicine, Beijing Jishuitan Hospital, Beijing 100035, China.
Jun
J
Wu
Department of laboratory medicine, Beijing Jishuitan Hospital, Beijing 100035, China.
Hui Ru
HR
Zhao
Department of laboratory medicine, Beijing Jishuitan Hospital, Beijing 100035, China.
Hui Ying
HY
Zhang
Department of laboratory medicine, Beijing Jishuitan Hospital, Beijing 100035, China.
Yan Ling
YL
Ge
Department of laboratory medicine, Beijing Jishuitan Hospital, Beijing 100035, China.
Shuang
S
Qiu
Department of laboratory medicine, People's Hospital, Peking University, Beijing 100044, China.
Jie
J
Zhao
Department of laboratory medicine, People's Hospital, Peking University, Beijing 100044, China.
Ying
Y
Song
Department of laboratory medicine, People's Hospital, Peking University, Beijing 100044, China.
Jing Zhong
JZ
Zhao
Department of laboratory medicine, People's Hospital, Peking University, Beijing 100044, China.
Song Song
SS
Lu
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000097:blood; D044466:Asian People; D001780:Blood Coagulation Tests / Q000592:standards*; D002681:China; D005260:Female; D064368:Healthy Volunteers; D006493:Heparin; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013917:Thrombin / Q000032:analysis; D055815:Young Adult
D006493:Heparin; D013917:Thrombin
Age; Calibrated automated thrombogram; Heparin; Low molecular weight heparin; Thrombin generation