Differential reward network functional connectivity in cannabis dependent and non-dependent users.
BACKGROUND
Emergent studies show that similar to other substances of abuse, cue-reactivity to cannabis is also associated with neural response in the brain's reward pathway (Filbey et al., 2009). However, the inter-relatedness of brain regions during cue-reactivity in cannabis users remains unknown.


METHODS
In this study, we conducted a series of investigations to determine functional connectivity during cue-reactivity in 71 cannabis users. First, we used psychophysiological interaction (PPI) analysis to examine coherent neural response to cannabis cues. Second, we evaluated whether these patterns of network functional connectivity differentiated dependent and non-dependent users. Finally, as an exploratory analysis, we determined the directionality of these connections via Granger connectivity analyses.


RESULTS
PPI analyses showed reward network functional connectivity with the nucleus accumbens (NAc) seed region during cue exposure. Between-group contrasts found differential effects of dependence status. Dependent users (N=31) had greater functional connectivity with amygdala and anterior cingulate gyrus (ACG) seeds while the non-dependent users (N=24) had greater functional connectivity with the NAc, orbitofrontal cortex (OFC) and hippocampus seeds. Granger analyses showed that hippocampal and ACG activation preceded neural response in reward areas.


CONCLUSIONS
Both PPI and Granger analyses demonstrated strong functional coherence in reward regions during exposure to cannabis cues in current cannabis users. Functional connectivity (but not regional activation) in the reward network differentiated dependent from non-dependent cannabis users. Our findings suggest that repeated cannabis exposure causes observable changes in functional connectivity in the reward network and should be considered in intervention strategies.
Center for BrainHealth, School of Behavioral and Brain Sciences, University of Texas at Dallas, Richardson, TX 75235, USA. Electronic address: Francesca.Filbey@utdallas.edu.
Francesca M
FM
Filbey
Center for BrainHealth, School of Behavioral and Brain Sciences, University of Texas at Dallas, Richardson, TX 75235, USA.
Joseph
J
Dunlop
D000328:Adult; D003463:Cues; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D002189:Marijuana Abuse / Q000503:physiopathology / Q000523:psychology*; D008385:Marijuana Smoking / Q000503:physiopathology / Q000523:psychology*; D009415:Nerve Net / Q000503:physiopathology*; D012201:Reward; D012959:Socioeconomic Factors; D055815:Young Adult
Craving; Cue-reactivity; Granger; Marijuana; PPI; Ventral striatum