Catalase prevents lipid peroxidation and enhances survival of caprine preantral follicles cryopreserved in a 1,2-propanediol-freezing medium.
The objectives of this study were to determine: 1) the optimal concentration (1.0 or 1.5 M) and duration of exposure (5, 10, or 20 min) of ovarian tissue to 1,2-propanediol (PROH) on morphology and viability of caprine preantral follicles; and 2) the effect of supplementing cryopreservation medium supplementation with Trolox(®) (0.1, 0.5, or 1.0 mM) or catalase (5, 10, or 20 IU/mL) on follicular morphology, viability, and lipid peroxidation. Cryopreservation decreased (p<0.05) percentages of normal follicles relative to the control (84%). Although supplementation of the cryopreservation medium (1.0 M PROH) with catalase (10 or 20 IU/mL) or Trolox(®) (0.1 mM) resulted in follicular morphology and viability similar to that in the controls (P>0.05), lipid peroxidation was reduced only when 20 IU/mL catalase was added to the cryopreservation medium.
1 Laboratory of Manipulation of Oocytes and Preantral Follicles: LAMOFOPA, Faculty of Veterinary, State University of Ceará , Fortaleza, Ceará, Brazil .
Hiedely K M
HK
Luz
Regiane R
RR
Santos
Livia S
LS
Wanderley
Luciana R
LR
Faustino
Cleidson M G
CM
Silva
Adeline A
AA
Carvalho
Claudio C
CC
Campello
Francielli W
FW
Santos
José R
JR
Figueiredo
Ana Paula R
AP
Rodrigues
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000009:adverse effects*; D005260:Female; D005615:Freezing; D006041:Goats; D015227:Lipid Peroxidation / Q000187:drug effects; D006080:Ovarian Follicle / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D019946:Propylene Glycol / Q000009:adverse effects*
D003451:Cryoprotective Agents; D019946:Propylene Glycol; D002374:Catalase