Pseudogenes as competitive endogenous RNAs: target prediction and validation.
Pseudogenes may regulate expression of their parental genes as well as other protein-coding genes through various mechanisms. One such mechanism is the ability to act as competitive endogenous RNA (ceRNA) and participate in microRNA-mediated cross-regulation. Here, we outline how to predict the targets of pseudogene ceRNAs bioinformatically and how to validate them experimentally.
Cancer Research Institute, Beth Israel Deaconess Cancer Center, Department of Medicine and Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS-401, Boston, MA, 02215, USA.
Florian A
FA
Karreth
Ugo
U
Ala
Paolo
P
Provero
Pier Paolo
PP
Pandolfi
D020413:3' Untranslated Regions; D019295:Computational Biology / Q000379:methods*; D015870:Gene Expression; D020868:Gene Silencing; D017930:Genes, Reporter; D023281:Genomics / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D011544:Pseudogenes / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D015203:Reproducibility of Results; D043244:Ribonuclease III / Q000172:deficiency / Q000235:genetics / Q000378:metabolism
D020413:3' Untranslated Regions; D035683:MicroRNAs; D012333:RNA, Messenger; D012313:RNA; D043244:Ribonuclease III