Dietary selenium deficiency exacerbates lipopolysaccharide-induced inflammatory response in mouse mastitis models.
Selenium (Se) is an essential micronutrient that plays a critical role in anti-inflammatory processes and antioxidant defense system. In this study, we investigated the effects of dietary selenium deficiency on lipopolysaccharide (LPS)-induced mastitis in mouse models. Se content in the liver was assessed by fluorescent atomic absorption spectrometry. Glutathione peroxidase (GPx) activity in the blood, myeloperoxidase (MPO) activity, tumor necrosis actor alpha (TNF-α), and interleukin (IL)-1β in the supernatant of the mammary tissue were determined according to the corresponding kits. Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expressions were evaluated by Western blotting. The results showed that the Se-deficient mouse model was successfully replicated, and selenium deficiency exacerbated mammary gland histopathology, increased the expressions of TNF-α and IL-1β, and facilitated the activation of iNOS and COX-2 in LPS-induced mouse mastitis. In conclusion, our studies demonstrated that selenium deficiency resulted in more severe inflammatory response in LPS-induced mouse mastitis.
College of Veterinary Medicine, Jilin University, 130062, Changchun, Jilin Province, People's Republic of China.
Zhengkai
Z
Wei
Minjun
M
Yao
Yimeng
Y
Li
Xuexiu
X
He
Zhengtao
Z
Yang
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008413:Mastitis / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D012643:Selenium / Q000172:deficiency*
D008070:Lipopolysaccharides; D012643:Selenium