Injection of Lactobacillus casei strain Shirota affects autonomic nerve activities in a tissue-specific manner, and regulates glucose and lipid metabolism in rats.
OBJECTIVE
Previously, it was observed that long-term ingestion of a probiotic strain Lactobacillus casei Shirota (LcS) ameliorates insulin resistance and glucose intolerance in rats fed a high-fat diet. In the present study, we examined its possible role in the autonomic nervous system during LcS-induced modulations in glucose and lipid metabolism or cardiovascular functions.


METHODS
The present study examined the effects of intragastric (IG) LcS injection on autonomic nerve tones in anesthetized rats by electrophysiological method.


RESULTS
We found that an IG injection of LcS suppressed neural activity of sympathetic nerves supplying the white adipose tissue of urethane-anesthetized rats in a dose-dependent manner, whereas sympathetic nerve outflow to brown adipose tissue was not affected by the IG LcS injection. Furthermore, the IG LcS injection reduced efferent sympathetic nerve outflow to the adrenal gland and liver, but did not alter gastric vagal nerve activity, renal sympathetic nerve activity, as well as mean arterial pressure. To test the involvement of afferent vagal nerves and the abdominal organs, we examined the adrenal sympathetic response to an LcS injection in rats with ablated afferent vagal nerves, and found that the adrenal sympathetic nerve response to LcS was inhibited in vagotomized rats. In addition, we found that oral ingestion of LcS attenuated the hyperglycemic response to glucose loading and blood glycerol levels in conscious rats.


CONCLUSIONS
Our data suggest that LcS might affect tissue-specific autonomic nerves through the afferent vagal nerve pathway to modulate glucose and lipid metabolism.
Department of Physiology II Kanazawa Medical University Uchinada Ishikawa Japan ; Department of Biomedical Sciences College of Life Sciences Ritsumeikan University Kusatsu Shiga Japan.
Mamoru
M
Tanida
Department of Biomedical Sciences College of Life Sciences Ritsumeikan University Kusatsu Shiga Japan.
Kazuki
K
Imanishi
Department of Biomedical Sciences College of Life Sciences Ritsumeikan University Kusatsu Shiga Japan.
Haruna
H
Akashi
Department of Physiology II Kanazawa Medical University Uchinada Ishikawa Japan.
Yasutaka
Y
Kurata
Yakult Central Institute for Microbiological Research Yakult Honsha Co., Ltd. Kunitachi Tokyo Japan.
Osamu
O
Chonan
Yakult Central Institute for Microbiological Research Yakult Honsha Co., Ltd. Kunitachi Tokyo Japan.
Eiichiro
E
Naito
Yakult Central Institute for Microbiological Research Yakult Honsha Co., Ltd. Kunitachi Tokyo Japan.
Satoru
S
Kunihiro
Yakult Central Institute for Microbiological Research Yakult Honsha Co., Ltd. Kunitachi Tokyo Japan.
Mitsuhisa
M
Kawai
Yakult Central Institute for Microbiological Research Yakult Honsha Co., Ltd. Kunitachi Tokyo Japan.
Akito
A
Kato-Kataoka
Department of Physiology II Kanazawa Medical University Uchinada Ishikawa Japan.
Toshishige
T
Shibamoto
Adipose tissue; Afferent vagal nerve; Hyperglycemia