Introducing improvements in the mass rearing of the housefly: biological, morphometric and genetic characterization of laboratory strains.
Understanding the biology of the housefly (Musca domestica L.) is crucial for the development of mass-rearing protocols in order to use this insect as a degradation agent for livestock waste. In this study, the biological and genetic differences between different laboratory strains of M. domestica were analysed. Additionally, hybrids were obtained by mixing the strains and their biological parameters were also measured. The three strains of M. domestica presented differences in their biological and morphological parameters, the main differences were: size, egg production and developmental time. The strain A (specimens from Central Europe) had the best qualities to be used in mass-rearing conditions: it produced the largest quantities of eggs (5.77±0.38 eggs per female per day), the individuals were larger (12.62±0.22 mg) and its developmental time was shorter (15.22±0.21 days). However, the strain C (specimens from SW Europe) produced the fewest eggs (3.15±0.42 eggs per female per day) and needed 18.16±0.49 days to develop from larva to adult, whilst the females from strain B (from South America) produced 4.25±0.47 eggs per day and needed 17.11±0.36 days to complete its development. Genetic analysis of the original laboratory strains showed four different mtDNA cytochrome c oxidase subunit I haplotypes. Statistical parsimony network analysis showed that the SW Europe and South-American strains shared haplotypes, whereas the Central Europe strain did not. Upon hybridizing the strains, variations in egg production and in developmental time were observed in between hybrids and pure strains, and when mixing Central European and South-American strains only males were obtained.
Departamento de Ciencias Ambientales y Recursos Naturales/Instituto Universitario CIBIO,Universidad de Alicante,Apdo. 99, E-03080 Alicante,Spain.
B
B
Pastor
Departamento de Ciencias Ambientales y Recursos Naturales/Instituto Universitario CIBIO,Universidad de Alicante,Apdo. 99, E-03080 Alicante,Spain.
A S
AS
Martínez-Sánchez
Finnish Museum of Natural History, University of Helsinki,Helsinki,Finland.
G A
GA
Ståhls
Departamento de Ciencias Ambientales y Recursos Naturales/Instituto Universitario CIBIO,Universidad de Alicante,Apdo. 99, E-03080 Alicante,Spain.
S
S
Rojo
D000704:Analysis of Variance; D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D000830:Animals, Laboratory / Q000235:genetics / Q000254:growth & development*; D001673:Biodegradation, Environmental; D001947:Breeding / Q000379:methods*; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D006239:Haplotypes / Q000235:genetics; D006793:Houseflies / Q000235:genetics / Q000254:growth & development*; D006824:Hybridization, Genetic / Q000235:genetics; D008297:Male; D008372:Manure; D010802:Phylogeny; D018154:Slovakia; D013030:Spain; D018709:Statistics, Nonparametric; D014687:Venezuela; D014921:Wings, Animal / Q000033:anatomy & histology
D008372:Manure; D003576:Electron Transport Complex IV