BSA Au clusters as a probe for enhanced fluorescence detection using multipulse excitation scheme.
Although BSA Au clusters fluoresce in red region (λmax: 650 nm), they are of limited use due to low fluorescence quantum yield (~6%). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime (> 1 µs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence. Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be used to develop biochemical assays and perform optical imaging with zero background.
Sangram L
SL
Raut
Ryan
R
Rich
Rafal
R
Fudala
R
R
Kokate
J D
JD
Kimball
Julian
J
Borejdo
Jamboor K
JK
Vishwanatha
Zygmunt
Z
Gryczynski
Center for Fluorescence Technologies and Nanomedicine, UNT Health Science Center, Fort Worth, TX, 76107l, USA. Ignacy.gryczynski@unthsc.edu.
Ignacy
I
Gryczynski
D045744:Cell Line, Tumor; D005453:Fluorescence; D005456:Fluorescent Dyes; D006046:Gold; D006801:Humans; D049329:Nanostructures; D012710:Serum Albumin, Bovine; D059629:Signal-To-Noise Ratio; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine; D006046:Gold