Incorporation of cisplatin into PEG-wrapped ultrapurified large-inner-diameter MWCNTs for enhanced loading efficiency and release profile.
The efficacy of carbon nanotubes based drug delivery systems (DDSs) has long been compromised by low drug loading efficiency and rapid release profile, especially for drugs encapsulated inside nanotubes. To address these deficiencies, the large-inner-diameter multi-walled carbon nanotubes (LID-MWCNTs) were employed to synthesize an innovative cisplatin (CDDP)-loaded DDS. A multi-step purification and oxidation procedure was developed to achieve ultrapurified oxidized LID-MWCNTs prior to entrapping CDDP. High molecular weight polyethylene glycol (PEG) was grafted onto the nanotubes to partially block the exit paths of drugs. As assessed by TGA and ICP-OES analyses, CDDP loading efficiency of resulting DDS was as high as 100.12%, and sustained release profile was obtained. Finally, the anticancer activity on CAL-27 cells was evaluated, and enhanced inhibition effect (IC50 values 3.93 μg/ml) was recorded. The successful inner-cavity-loading of LID-MWCNTs also provides a basis for establishing more complicated multi-functional DDSs.
School of Stomatology, Tianjin Medical University, China No.12, Qixiangtai Rd. Heping District, Tianjin 610041, China. Electronic address: suilei@tmu.edu.cn.
Lei
L
Sui
School of Stomatology, Tianjin Medical University, China No.12, Qixiangtai Rd. Heping District, Tianjin 610041, China. Electronic address: ytdentist@outlook.com.
Tao
T
Yang
School of Stomatology, Tianjin Medical University, China No.12, Qixiangtai Rd. Heping District, Tianjin 610041, China.
Ping
P
Gao
School of Stomatology, Tianjin Medical University, China No.12, Qixiangtai Rd. Heping District, Tianjin 610041, China.
Ai
A
Meng
School of Stomatology, Tianjin Medical University, China No.12, Qixiangtai Rd. Heping District, Tianjin 610041, China.
Pingting
P
Wang
School of Stomatology, Tianjin Medical University, China No.12, Qixiangtai Rd. Heping District, Tianjin 610041, China.
Zhenzhen
Z
Wu
Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, China No.14, 3rd Section of Ren Min Nan Rd., Chengdu, Sichuan 610041, China. Electronic address: ferowang@hotmail.com.
Jian
J
Wang
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D065546:Drug Liberation; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
D000970:Antineoplastic Agents; D004337:Drug Carriers; D037742:Nanotubes, Carbon; D011092:Polyethylene Glycols; D002945:Cisplatin
Carbon nanotubes; Cisplatin; Cytotoxicity; Drug delivery system; Drug loading efficiency; Release profile