Avian prostatic acid phosphatase: estrogen regulation in the oviduct and epithelial cell-derived ovarian carcinomas.
Prostatic acid phosphatase (ACPP) is a glycoprotein that is mainly synthesized and secreted by glandular epithelial cells (GE) of the prostate, and it is well known as a biomarker for prostate cancer. Although ACPP was used as prognostic/diagnostic indicator and studied to elucidate regulatory mechanism(s) during several decades in humans, its role is not clearly understood. Gene profiling data using a chicken DNA microarray revealed that ACPP increased significantly during remodeling and recrudescence of the oviduct in response to estrogen. Thus, in this study, we investigated the expression and hormonal regulation of ACPP gene in the reproductive tracts of chickens. ACPP was specifically detected in the luminal cells (LE) and GE of chicken oviduct, and diethylstilbestrol (a synthetic nonsteroidal estrogen) stimulated its expression during development of the oviduct. In addition, ACPP mRNA and protein were localized to LE and GE during the regeneration phase of the oviduct of laying hens during induced molting. Furthermore, ACPP mRNA and protein were abundant in GE of ovarian carcinoma, but not in normal ovaries. Moreover, strong expression of ACPP protein was detected in epithelial cells of cancerous ovaries from women. Collectively, results of the present study are the first to show that ACPP is a novel estrogen-stimulated gene in the oviductal epithelial cells of the chicken and that its expression increases significantly in epithelial cells of ovarian carcinoma, which indicates that it may be a candidate biomarker for diagnosis of epithelia-derived ovarian cancer in women.
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Hyocheol
H
Bae
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Whasun
W
Lim
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Seung-Min
SM
Bae
Center for Animal Biotechnology and Genomics and Department of Animal Science, Texas A&M University, College Station, Texas.
Fuller W
FW
Bazer
Department of Biomedical Science, CHA University, Seoul, Republic of Korea youngsokchoi@cha.ac.kr.
Youngsok
Y
Choi
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea ghsong@korea.ac.kr.
Gwonhwa
G
Song
D000135:Acid Phosphatase; D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma / Q000201:enzymology*; D002645:Chickens; D004054:Diethylstilbestrol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D010051:Ovarian Neoplasms / Q000201:enzymology*; D010057:Oviducts / Q000187:drug effects / Q000201:enzymology*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*
D030161:Avian Proteins; D014408:Biomarkers, Tumor; D004054:Diethylstilbestrol; D000135:Acid Phosphatase; C428439:prostatic acid phosphatase; D017027:Protein Tyrosine Phosphatases
ACPP; carcinoma; chicken; estrogen; ovary; oviduct