Efficient fluorescence resonance energy transfer between oppositely charged CdTe quantum dots and gold nanoparticles for turn-on fluorescence detection of glyphosate.
We designed a turn-on fluorescence assay for glyphosate based on the fluorescence resonance energy transfer (FRET) between negatively charged CdTe quantum dots capped with thioglycolic acid (TGA-CdTe-QDs) and positively charged gold nanoparticles stabilized with cysteamine (CS-AuNPs). Oppositely charged TGA-CdTe-QDs and CS-AuNPs can form FRET donor-acceptor assemblies due to electrostatic interactions, which effectively quench the fluorescence intensity of TGA-CdTe-QDs. The presence of glyphosate could induce the aggregation of CS-AuNPs through electrostatic interactions, resulting in the fluorescence recovery of the quenched QDs. This FRET-based method has been successfully utilized to detect glyphosate in apples with satisfactory results. The detection limit for glyphosate was 9.8 ng/kg (3σ), with the linear range of 0.02-2.0 μg/kg. The attractive sensitivity was obtained due to the efficient FRET and the superior fluorescence properties of QDs. The proposed method is a promising approach for rapid screening of glyphosate in real samples.
Department of Food Quality and Safety, Jilin University, Changchun 130062, China.
Jiajia
J
Guo
Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, China.
Yan
Y
Zhang
Department of Food Quality and Safety, Jilin University, Changchun 130062, China.
Yeli
Y
Luo
Department of Food Quality and Safety, Jilin University, Changchun 130062, China.
Fei
F
Shen
Department of Food Quality and Safety, Jilin University, Changchun 130062, China. Electronic address: sunchuny@jlu.edu.cn.
Chunyan
C
Sun
D019187:Cadmium Compounds / Q000737:chemistry*; D019343:Citric Acid / Q000737:chemistry; D004563:Electrochemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D005506:Food Contamination; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D027845:Malus; D053768:Metal Nanoparticles / Q000737:chemistry*; D045663:Quantum Dots; D013056:Spectrophotometry, Ultraviolet; D013691:Tellurium / Q000737:chemistry*; D013864:Thioglycolates / Q000737:chemistry; D014867:Water / Q000737:chemistry; D000097797:Glyphosate
D019187:Cadmium Compounds; D019343:Citric Acid; D005456:Fluorescent Dyes; D005998:Glycine; D006046:Gold; D013691:Tellurium; D013864:Thioglycolates; D014867:Water; D000097797:Glyphosate; C017487:2-mercaptoacetate; C028337:cadmium telluride
CdTe quantum dots; Fluorescence resonance energy transfer; Glyphosate; Gold nanoparticles