Acceleration of bone union after structural bone grafts with a collagen-binding basic fibroblast growth factor anchored-collagen sheet for critical-size bone defects.
Bone allografts are commonly used for the repair of critical-size bone defects. However, the loss of cellular activity in processed grafts markedly reduces their healing potential compared with autografts. To overcome this obstacle, we developed a healing system for critical-size bone defects that consists of overlaying an implanted bone graft with a collagen sheet (CS) loaded with basic fibroblast growth factor (bFGF) fused to the collagen-binding domain derived from a Clostridium histolyticum collagenase (CB-bFGF). In a murine femoral defect model, defect sites treated with CS/CB-bFGF had a significantly larger callus volume than those treated with CS/native bFGF. In addition, treatment with CS/CB-bFGF resulted in the rapid formation of a hard callus bridge and a larger total callus volume at the host-graft junction than treatment with CS/bFGF. Our results suggest that the combined use of CS and CB-bFGF helps accelerate the union of allogenic bone grafts.
Department of Orthopaedic Surgery, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.
Masaki
M
Ueno
Kentaro
K
Uchida
Wataru
W
Saito
Osamu
O
Matsushita
Mizuki
M
Yogoro
Nozomu
N
Nishi
Takayuki
T
Ogura
Shunji
S
Hattori
Gen
G
Inoue
Keisuke
K
Tanaka
Naonobu
N
Takahira
Masashi
M
Takaso
D000818:Animals; D016025:Bone Transplantation / Q000295:instrumentation* / Q000379:methods; D003094:Collagen / Q000737:chemistry*; D004343:Drug Implants / Q000008:administration & dosage / Q000737:chemistry; D005264:Femoral Fractures / Q000503:physiopathology* / Q000628:therapy*; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000737:chemistry; D017102:Fracture Healing / Q000187:drug effects / Q000502:physiology; D017759:Fractures, Malunited / Q000175:diagnosis / Q000503:physiopathology / Q000628:therapy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011485:Protein Binding; D051381:Rats; D016896:Treatment Outcome
D004343:Drug Implants; D016222:Fibroblast Growth Factor 2; D003094:Collagen