Tissue imprints: assessing their potential for routine biobanking collection.
Biomedical research depends on the availability of good quality biospecimens. Unfortunately, certain specimens are scarce due to disease rarity or size restrictions of surgical materials. To increase access to limited surgical specimens, Biobanks need to reassess and adjust their collection programs. We evaluated the feasibility of adapting "touch imprints" to gain access to limited surgical specimens as well as to maximize the use of "precious" specimens. We utilized 12 kidney samples for touch imprints on defined areas of microscope glass slides and FTA paper. DNA was isolated from glass slides on the day of preparation, Day 0, and from glass slide and FTA paper preparations after two weeks of storage at room temperature and -80°C. Yield and purity of DNA from reference kidney samples were compared to DNA from the touch imprints and the quality determined by real-time PCR using the amplification of Cyclophilin A (Cyc A) as an index. DNA quality for glass slides at Day 0 was not significantly different from DNA after two weeks at room temperature (glass at room temperature; p=0.111 and 0.097, yield and purity, respectively) and after two weeks at -80°C (glass -80°C; p=0.358 and 0.281, yield and purity, respectively). Glass slide DNA at room temperature and -80°C were not significantly different (p=0.795 and 0.146 for yield and purity, respectively). DNA from FTA paper at room temperature and from FTA paper at -80°C were significantly different from glass at room temperature and glass at -80°C (p=0.002, respectively). Threshold values for Cyc A were ≤28 for the reference DNA and ≤32 for DNA from glass and FTA paper. This study demonstrates that touch preparations on microscope glass slides and FTA paper can provide sufficient and good quality DNA suitable for PCR. Touch imprints could therefore be adopted by biobanks to collect and bank biological materials from limited surgical specimens.
1 Walter Reed National Military Medical Center , Bethesda, Maryland.
Renata
R
Greenspan
Amy
A
O'Donnell
Jeff
J
Meyer
Jennifer
J
Kane
Kim
K
Mamula
Sue
S
Lubert
Brenda
B
Deyarmin
Caroline
C
Larson
Sean
S
Rigby
Amber
A
Greenawalt
Negin
N
Vatanian
Richard
R
Mural
Craig
C
Shriver
Stella
S
Somiari
D000328:Adult; D000368:Aged; D021984:Cyclophilin A / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D006801:Humans; D007668:Kidney / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011309:Preservation, Biological; D013048:Specimen Handling / Q000379:methods*; D014015:Tissue Banks; D055815:Young Adult
D021984:Cyclophilin A