A rational approach for generating cardiac troponin I selective Spiegelmers.
We report the first protein selective Spiegelmers of diagnostic relevance by rational identification of a target epitope and reverse screening of Spiegelmer candidates following the selection procedure. Application of the presented approach resulted in isolation of cardiac troponin I selective Spiegelmers with low nanomolar dissociation constant and functionality in serum.
Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tűzoltó u. 37-47, H-1094 Budapest, Hungary. meszaros.tamas@med.semmelweis-univ.hu.
Zsuzsanna
Z
Szeitner
Gergely
G
Lautner
Szilvia K
SK
Nagy
Róbert E
RE
Gyurcsányi
Tamás
T
Mészáros
D052158:Aptamers, Peptide / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D009202:Cardiomyopathies / Q000378:metabolism; D000939:Epitopes; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D009113:Muramidase / Q000737:chemistry; D011485:Protein Binding; D019210:Troponin I / Q000737:chemistry*
D052158:Aptamers, Peptide; D015415:Biomarkers; D000939:Epitopes; D007202:Indicators and Reagents; D019210:Troponin I; D009113:Muramidase