Involvement of the Wbp pathway in the biosynthesis of Porphyromonas gingivalis lipopolysaccharide with anionic polysaccharide.
The periodontal pathogen Porphyromonas gingivalis has two different lipopolysaccharide (LPS) molecules, O-LPS and A-LPS. We have recently shown that P. gingivalis strain HG66 lacks A-LPS. Here, we found that introduction of a wild-type wbpB gene into strain HG66 restored formation of A-LPS. Sequencing of the wbpB gene from strain HG66 revealed the presence of a nonsense mutation in the gene. The wbpB gene product is a member of the Wbp pathway, which plays a role in the synthesis of UDP-ManNAc(3NAc)A in Pseudomonas aeruginosa; UDP-ManNAc(3NAc)A is sequentially synthesized by the WbpA, WbpB, WbpE, WbpD and WbpI proteins. We then determined the effect of the PGN_0002 gene, a wbpD homolog, on the biosynthesis of A-LPS. A PGN_0002-deficient mutant demonstrated an A-LPS biosynthesis deficiency. Taken together with previous studies, the present results suggest that the final product synthesized by the Wbp pathway is one of the sugar substrates necessary for the biosynthesis of A-LPS.
Division of Microbiology and Oral Infection, Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8588, Japan.
Mikio
M
Shoji
Division of Microbiology and Oral Infection, Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8588, Japan.
Keiko
K
Sato
Division of Microbiology and Oral Infection, Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8588, Japan.
Hideharu
H
Yukitake
Division of Microbiology and Oral Infection, Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8588, Japan.
Mariko
M
Naito
Division of Microbiology and Oral Infection, Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8588, Japan.
Koji
K
Nakayama
D000818:Animals; D000838:Anions / Q000737:chemistry*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D016866:Bacteroidaceae Infections / Q000378:metabolism* / Q000382:microbiology; D004912:Erythrocytes / Q000378:metabolism; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011134:Polysaccharides / Q000737:chemistry*; D016966:Porphyromonas gingivalis / Q000502:physiology; D012756:Sheep; D015398:Signal Transduction; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
D000838:Anions; D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D011134:Polysaccharides; D014539:Uridine Diphosphate Sugars; C059191:uridine diphosphate N-acetylmannosamine