Isolation of Paenibacillus glucanolyticus from pulp mill sources with potential to deconstruct pulping waste.
Black liquor is a pulping waste generated by the kraft process that has potential for downstream bioconversion. A microorganism was isolated from a black liquor sample collected from the Department of Forest Biomaterials at North Carolina State University. The organism was identified as Paenibacillus glucanolyticus using 16S rRNA sequence analysis and was shown to be capable of growth on black liquor as the sole carbon source based on minimal media growth studies. Minimal media growth curves demonstrated that this facultative anaerobic microorganism can degrade black liquor as well as cellulose, hemicellulose, and lignin. Gas chromatography-mass spectrometry was used to identify products generated by P. glucanolyticus when it was grown anaerobically on black liquor. Fermentation products which could be converted into high-value chemicals such as succinic, propanoic, lactic, and malonic acids were detected.
Department of Plant and Microbial Biology, 4550A Thomas Hall, Campus Box 7612, North Carolina State University, Raleigh, NC 27695, USA; Department of Forest Biomaterials, Biltmore Hall, Campus Box 8005, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: sklambet@ncsu.edu.
Stephanie L
SL
Mathews
Department of Forest Biomaterials, Biltmore Hall, Campus Box 8005, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: jjpawlak@ncsu.edu.
Joel J
JJ
Pawlak
Department of Plant and Microbial Biology, 4550A Thomas Hall, Campus Box 7612, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: amy_grunden@ncsu.edu.
Amy M
AM
Grunden
D000693:Anaerobiosis / Q000187:drug effects; D018533:Biomass; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D008031:Lignin / Q000378:metabolism; D056507:Paenibacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D010209:Paper; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature
D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; C036909:lignocellulose; D002244:Carbon; D008031:Lignin
Biodegradation (bacterial degradation); Black liquor; Lignin; Paenibacillus glucanolyticus; Pulp mill waste