Isolation of yeast nucleic acids.
Saccharomyces cerevisiae is a well-established model organism used to study multiple facets of eukaryotic organisms. The manipulation and isolation of DNA is a key element of basic genetic research. Meanwhile, the isolation of RNA is required for the study of transcriptional regulation. Presented in this chapter are fast and efficient methods of isolating genomic and plasmid DNA and total RNA that is capable of being utilized for a variety of genetic studies such as restriction analysis, northern and southern blotting, and real-time reverse-transcriptase PCR. Plasmids isolated via this method are also of sufficient quality to be transformed into E. coli for further genetic manipulation and study.
Department of Microbiology and Immunology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK, Canada, S7N 5E5.
Michael
M
Biss
Michelle D
MD
Hanna
Wei
W
Xiao
D004271:DNA, Fungal / Q000302:isolation & purification*; D036281:Genetic Research; D008967:Molecular Biology / Q000379:methods*; D012313:RNA / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000302:isolation & purification
D004271:DNA, Fungal; D012313:RNA