Biologically synthesized silver nanoparticles induce neuronal differentiation of SH-SY5Y cells via modulation of reactive oxygen species, phosphatases, and kinase signaling pathways.
Nano-scale materials are noted for unique properties, distinct from those of their bulk material equivalents. In this study, we prepared spherical silver nanoparticles (AgNPs) with an average size of about 30 nm and tested their potency to induce neuronal differentiation of SH-SY5Y cells. Human neuroblastoma SH-SY5Y cells are considered an ideal in vitro model for studying neurogenesis, as they can be maintained in an undifferentiated state or be induced to differentiate into neuron-like phenotypes in vitro by several differentiation-inducing agents. Treatment of SH-SY5Y cells by biologically synthesized AgNPs led to cell morphological changes and significant increase in neurite length and enhanced the expression of neuronal differentiation markers such as Map-2, β-tubulin III, synaptophysin, neurogenin-1, Gap-43, and Drd-2. Furthermore, we observed an increase in generation of intracellular reactive oxygen species (ROS), activation of several kinases such as ERK and AKT, and downregulation of expression of dual-specificity phosphatases (DUSPs) in AgNPs-exposed SH-SY5Y cells. Our results suggest that AgNPs modulate the intracellular signaling pathways, leading to neuronal differentiation, and could be applied as promising nanomaterials for stem cell research and therapy.
Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal model and Stem cell Institute (IDASI), Konkuk University, Seoul, Republic of Korea.
Ahmed Abdal
AA
Dayem
BongWoo
B
Kim
Sangiliyandi
S
Gurunathan
Hye Yeon
HY
Choi
Gwangmo
G
Yang
Subbroto Kumar
SK
Saha
Dawoon
D
Han
Jihae
J
Han
Kyeongseok
K
Kim
Jin-Hoi
JH
Kim
Ssang-Goo
SG
Cho
D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D054637:Dual-Specificity Phosphatases / Q000235:genetics / Q000378:metabolism; D019922:GAP-43 Protein / Q000235:genetics / Q000378:metabolism; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017448:Receptors, Dopamine D2 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D012834:Silver / Q000737:chemistry*; D016708:Synaptophysin / Q000235:genetics / Q000378:metabolism; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
D051792:Basic Helix-Loop-Helix Proteins; D054637:Dual-Specificity Phosphatases; D019922:GAP-43 Protein; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D010744:Phosphoric Monoester Hydrolases; D017382:Reactive Oxygen Species; D017448:Receptors, Dopamine D2; D012834:Silver; D016708:Synaptophysin; D014404:Tubulin; C581293:DRD2 protein, human; C489788:MAP2 protein, human; C105389:NEUROG1 protein, human
Dual-specificity phosphatases (DUSPs); Neuroblastoma; Neuronal differentiation; Reactive oxygen species; Silver nanoparticles