Biological protein-resistance layer construction of recombinant hirudin on polymethyl methacrylate IOL surface.
In this article, the surface of intraocular len material PMMA was first aminated for activation on which some polar groups generated such as C-N, COO(-), -OH, NH3(+), etc. Then the anticoagulant drugs recombinant hirudin (rH) was grafted with amido bonds to look forward to resist the adsorption of nonspecific protein or cells in tear, even the cataract. The detailed analysis and discussion about the grafting quantity, molography, wettability, electric charges, chemical structure, and the dynamic adsorption of protein Fn on the material surface were carried on by the technology of ultraviolet photometric, contact angle, solid Zeta potential, X-ray photoelectron spectroscopy, and quartz crystal microbalance. The surface with a certain amount of rH modification existed more hydrophilic due to the amphiphilic structure than before, on which the protein adsorption was the most unstable. The results indicated that the rH modification improved the resistance of PMMA to nonspecific adsorption of protein Fn to achieve the expectative effect.
National Engineering Research Center for Human Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510640, People's Republic of China; Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, People's Republic of China.
Zhiwen
Z
Zheng
Yan
Y
Jiao
Li
L
Ren
Yingjun
Y
Wang
D000327:Adsorption; D000641:Ammonia / Q000737:chemistry; D000925:Anticoagulants / Q000737:chemistry*; D003460:Crystallization; D005353:Fibronectins / Q000737:chemistry; D006629:Hirudins / Q000737:chemistry*; D007910:Lenses, Intraocular; D010455:Peptides / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011791:Quartz; D011994:Recombinant Proteins / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties; D014466:Ultraviolet Rays; D017155:Wettability
D000925:Anticoagulants; D005353:Fibronectins; D006629:Hirudins; D010455:Peptides; D011994:Recombinant Proteins; D011791:Quartz; D000641:Ammonia; D019904:Polymethyl Methacrylate
PMMA; protein resistance; recombinant hirudin; wettability; zeta potential