Distinct features of the histone core structure in nucleosomes containing the histone H2A.B variant.
Nucleosomes containing a human histone variant, H2A.B, in an aqueous solution were analyzed by small-angle neutron scattering utilizing a contrast variation technique. Comparisons with the canonical H2A nucleosome structure revealed that the DNA termini of the H2A.B nucleosome are detached from the histone core surface, and flexibly expanded toward the solvent. In contrast, the histone tails are compacted in H2A.B nucleosomes compared to those in canonical H2A nucleosomes, suggesting that they bind to the surface of the histone core and/or DNA. Therefore, the histone tail dynamics may function to regulate the flexibility of the DNA termini in the nucleosomes.
Research Reactor Institute, Kyoto University, Kumatori, Osaka 590-0494, Japan. Electronic address: sugiyama@rri.kyoto-u.ac.jp.
Masaaki
M
Sugiyama
(‡)Laboratory of Structural Biology, Waseda University, Shinjuku-ku, Tokyo 162-8480, Japan.
Yasuhiro
Y
Arimura
(‡)Laboratory of Structural Biology, Waseda University, Shinjuku-ku, Tokyo 162-8480, Japan.
Kazuyoshi
K
Shirayama
(‡)Laboratory of Structural Biology, Waseda University, Shinjuku-ku, Tokyo 162-8480, Japan.
Risa
R
Fujita
Research Reactor Institute, Kyoto University, Kumatori, Osaka 590-0494, Japan.
Yojiro
Y
Oba
Research Reactor Institute, Kyoto University, Kumatori, Osaka 590-0494, Japan.
Nobuhiro
N
Sato
Research Reactor Institute, Kyoto University, Kumatori, Osaka 590-0494, Japan.
Rintaro
R
Inoue
Graduate School of Medical Life Science, Yokohama City University, Tsurumi, Yokohama 230-0045, Japan.
Takashi
T
Oda
Graduate School of Medical Life Science, Yokohama City University, Tsurumi, Yokohama 230-0045, Japan.
Mamoru
M
Sato
ISIS Facility, STFC Rutherford Appleton Laboratory, Didcot, OX11 0QX, UK.
Richard K
RK
Heenan
(‡)Laboratory of Structural Biology, Waseda University, Shinjuku-ku, Tokyo 162-8480, Japan. Electronic address: kurumizaka@waseda.jp.
Hitoshi
H
Kurumizaka
D006657:Histones / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism*; D012399:Rotation
D006657:Histones; D009707:Nucleosomes