Effect of sterilization methods on cell binding activity of surface-immobilized fibronectin.
The effect of sterilization methods on biological activity of fibronectin on the surface of biomaterials was elaborated in the present study. Sterile protein- modified biomaterials were fabricated by microfilter filtration and UV irradiation, respectively. UV irradiation altered the conformation of surface- adsorbed fibronectin and further affected the attachment, morphology and biological function of endothelial cells. However, microfilter filtration did not to change the normal conformation of fibronectin, or the proliferation and biological function of endothelial cells, indicating that microfilter filtration sterilization is the most suitable method for protein-substrate.
Intensive Care Unit, Xiasha Hospital, Hangzhou 310019, Zhejiang, China.
Hai Bo
HB
Xing
Department of Medical Oncology, Sir Run Run Shaw Hospital Affiliated to Zhejiang University Medical School, Hangzhou 310019, Zhejiang, China.
Qin
Q
Pan
Department of Medical Oncology, Sir Run Run Shaw Hospital Affiliated to Zhejiang University Medical School, Hangzhou 310019, Zhejiang, China.
Kai Feng
KF
Wang
Department of Medical Oncology, Sir Run Run Shaw Hospital Affiliated to Zhejiang University Medical School, Hangzhou 310019, Zhejiang, China.
Fang
F
Lou
Department of Medical Oncology, Sir Run Run Shaw Hospital Affiliated to Zhejiang University Medical School, Hangzhou 310019, Zhejiang, China.
Da
D
Li
D002448:Cell Adhesion / Q000528:radiation effects; D005353:Fibronectins / Q000528:radiation effects*; D005374:Filtration; D019736:Prostheses and Implants / Q000382:microbiology*; D013242:Sterilization / Q000379:methods*; D014466:Ultraviolet Rays
D005353:Fibronectins