"Turn-on" fluorescent dipodal chemosensor for nano-molar detection of Zn(2+): application in living cells imaging.
A malonohydrazide derivative bearing an imine and phenolic group was synthesized and had a high affinity and selectivity towards Zn(2+). The recognition properties of receptor 1 were evaluated using absorbance and fluorescence spectroscopy. The 1:1 stoichiometry of 1.Zn(2+) was confirmed from job's plot, mass spectra and density functional theory (DFT). The detection of Zn(2+) in intracellular environment of HeLa cell through confocal microscopy was successfully applied for live cell imaging.
School of Chemical Sciences, North Maharashtra University, Jalgaon 425001, Maharashtra, India; School of Environmental and Earth Sciences, North Maharashtra University, Jalgaon 425001, Maharashtra, India.
Umesh
U
Fegade
Department of Chemistry, Indian Institute of Tecnhology, Ropar, Rupanagar, Punjab, India.
Hemant
H
Sharma
Molecular Biology and Human Genetics Laboratory, Department of Zoology, The University of Burdwan, Burdwan 713104, West Bengal, India.
Banashree
B
Bondhopadhyay
Molecular Biology and Human Genetics Laboratory, Department of Zoology, The University of Burdwan, Burdwan 713104, West Bengal, India.
Anupam
A
Basu
School of Environmental and Earth Sciences, North Maharashtra University, Jalgaon 425001, Maharashtra, India.
Sanjay
S
Attarde
Department of Chemistry, Indian Institute of Tecnhology, Ropar, Rupanagar, Punjab, India.
Narinder
N
Singh
School of Chemical Sciences, North Maharashtra University, Jalgaon 425001, Maharashtra, India. Electronic address: kuwaras@gmail.com.
Anil
A
Kuwar
D015374:Biosensing Techniques; D002412:Cations; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007097:Imines / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008670:Metals / Q000737:chemistry; D018613:Microscopy, Confocal; D008968:Molecular Conformation; D053758:Nanoparticles; D019800:Phenol / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry; D015032:Zinc / Q000737:chemistry*
D002412:Cations; D005456:Fluorescent Dyes; D007097:Imines; D008670:Metals; D014867:Water; D019800:Phenol; D015032:Zinc
Density functional theory; Fluorescence spectroscopy; Live cell imaging; Zinc recognition