Three new lignan glycosides from the fruits of Forsythia suspense.
Two new lignan glycosides forsythiayanoside A (1) and forsythiayanoside B (2), one new stereoisomer of lignan glycoside (+)-8-hydroxyepipinoresinol-4-O-β-D-glucopyranoside (3), together with seven known compounds (4-10), were isolated from the fruits of Forsythia suspense (Thunb.) Vahl. Compounds 4 and 10 were isolated from this species for the first time. The structures of 1-3 were elucidated on the basis of chemical and spectral analyses, including 1D and 2D NMR data and HR-ESI-MS. The absolute configurations were determined by the circular dichroism method. All isolates were tested for their cytotoxicities against five human cancer cell lines (A549, Colo-205, Hep-3B, HL60, and KB). In particular, compound 3 showed significant cytotoxicity with IC50 values of 9.48, 7.75, 0.59, 4.06, and 38.38 μM, respectively.
a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , China.
Xin-Jia
XJ
Yan
Ying
Y
Peng
Zhi-Xiang
ZX
Liu
Jing
J
Wen
Qing-Bo
QB
Liu
Ling-Zhi
LZ
Li
Shao-Jiang
SJ
Song
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D031657:Forsythia / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D007624:KB Cells; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013237:Stereoisomerism
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D017705:Lignans; C000591871:forsythiayanoside A; C000591872:forsythiayanoside B
CD; Forsythia suspense; NMR; cytotoxic activity; lignan glycosides