Expression of HPV-16 E6 protein and p53 inactivation increases the uterine cervical cancer invasion.
BACKGROUND
Human papillomavirus infection (HPV-16) and expression of HPV E6 protein are the major risk factors of cervical cancer. Studies reported that expression of E6 protein adversely affect the function of p53 and thus involved in tumor progression. In the present study, we made an attempt to analyze the prevalence of HPV-16 association, E6 and p53 expression in cervical cancer tissues.


METHODS
Uterine cervical cancer (n=10) and corresponding normal epithelium tissues (n=10) were collected at the time of surgery. The HPV-16 integration and E6 expression were analyzed by PCR and immunohistostaining. Further, p53 and Ki67 expression were analyzed by immunohistostaining. Telomerase was detected using a modified TRAP (telomerase repeat amplification protocol) assay.


RESULTS
We have found that almost 90% of the collected cervical cancer DNA samples showed positivity to HPV-16 and more than 60% of DNA samples were E6 positive. Further, these tissues were highly positive to p53 and Ki67 protein which contribute to apoptosis resistance and increased cell proliferation. We also found elevated level of telomerase expression in cancer tissues compared to control.


CONCLUSIONS
The association of HPV infection and E6 expression increases the risk of cervical cancer. Further, E6 expression is the cause for inactivation of p53 in tumor tissues and deregulated cell proliferation and thus favors for tumor invasion.
Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, P.R. China.
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Yang
Urumqi MCH, Urumqi, P.R. China.
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Lu
D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation; D016158:Genes, p53 / Q000235:genetics*; D052162:Human papillomavirus 16 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis* / Q000235:genetics; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D019098:Telomerase / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*
C068651:E6 protein, Human papillomavirus type 16; D019394:Ki-67 Antigen; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins; D019098:Telomerase