Determination of rice syrup adulterant concentration in honey using three-dimensional fluorescence spectra and multivariate calibrations.
To rapidly and efficiently detect the presence of adulterants in honey, three-dimensional fluorescence spectroscopy (3DFS) technique was employed with the help of multivariate calibration. The data of 3D fluorescence spectra were compressed using characteristic extraction and the principal component analysis (PCA). Then, partial least squares (PLS) and back propagation neural network (BP-ANN) algorithms were used for modeling. The model was optimized by cross validation, and its performance was evaluated according to root mean square error of prediction (RMSEP) and correlation coefficient (R) in prediction set. The results showed that BP-ANN model was superior to PLS models, and the optimum prediction results of the mixed group (sunflower±longan±buckwheat±rape) model were achieved as follow: RMSEP=0.0235 and R=0.9787 in the prediction set. The study demonstrated that the 3D fluorescence spectroscopy technique combined with multivariate calibration has high potential in rapid, nondestructive, and accurate quantitative analysis of honey adulteration.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: qschen@ujs.edu.cn.
Quansheng
Q
Chen
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Shuai
S
Qi
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Huanhuan
H
Li
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Xiaoyan
X
Han
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Qin
Q
Ouyang
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Jiewen
J
Zhao
D000465:Algorithms; D002138:Calibration; D005453:Fluorescence; D005504:Food Analysis / Q000191:economics / Q000379:methods*; D063427:Food Quality; D006722:Honey / Q000032:analysis*; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D016571:Neural Networks, Computer; D012275:Oryza / Q000737:chemistry*; D025341:Principal Component Analysis; D013050:Spectrometry, Fluorescence / Q000191:economics / Q000379:methods*
Adulteration; Honey; Multivariate calibration; Rice syrup; Three-dimensional fluorescence spectra (3DFS)