Analysis of insecticide resistance-related genes of the Carmine spider mite Tetranychus cinnabarinus based on a de novo assembled transcriptome.
The carmine spider mite (CSM), Tetranychus cinnabarinus, is an important pest mite in agriculture, because it can develop insecticide resistance easily. To gain valuable gene information and molecular basis for the future insecticide resistance study of CSM, the first transcriptome analysis of CSM was conducted. A total of 45,016 contigs and 25,519 unigenes were generated from the de novo transcriptome assembly, and 15,167 unigenes were annotated via BLAST querying against current databases, including nr, SwissProt, the Clusters of Orthologous Groups (COGs), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). Aligning the transcript to Tetranychus urticae genome, the 19255 (75.45%) of the transcripts had significant (e-value <10-5) matches to T. urticae DNA genome, 19111 sequences matched to T. urticae proteome with an average protein length coverage of 42.55%. Core Eukaryotic Genes Mapping Approach (CEGMA) analysis identified 435 core eukaryotic genes (CEGs) in the CSM dataset corresponding to 95% coverage. Ten gene categories that relate to insecticide resistance in arthropod were generated from CSM transcriptome, including 53 P450-, 22 GSTs-, 23 CarEs-, 1 AChE-, 7 GluCls-, 9 nAChRs-, 8 GABA receptor-, 1 sodium channel-, 6 ATPase- and 12 Cyt b genes. We developed significant molecular resources for T. cinnabarinus putatively involved in insecticide resistance. The transcriptome assembly analysis will significantly facilitate our study on the mechanism of adapting environmental stress (including insecticide) in CSM at the molecular level, and will be very important for developing new control strategies against this pest mite.
Key Laboratory of Entomology and Pest Control Engineering of Chongqing, College of Plant Protection, Southwest University, Chongqing, China.
Zhifeng
Z
Xu
Key Laboratory of Entomology and Pest Control Engineering of Chongqing, College of Plant Protection, Southwest University, Chongqing, China.
Wenyi
W
Zhu
Key Laboratory of Entomology and Pest Control Engineering of Chongqing, College of Plant Protection, Southwest University, Chongqing, China.
Yanchao
Y
Liu
Key Laboratory of Entomology and Pest Control Engineering of Chongqing, College of Plant Protection, Southwest University, Chongqing, China.
Xing
X
Liu
Key Laboratory of Entomology and Pest Control Engineering of Chongqing, College of Plant Protection, Southwest University, Chongqing, China.
Qiushuang
Q
Chen
Key Laboratory of Entomology and Pest Control Engineering of Chongqing, College of Plant Protection, Southwest University, Chongqing, China.
Miao
M
Peng
Key Laboratory of Entomology and Pest Control Engineering of Chongqing, College of Plant Protection, Southwest University, Chongqing, China.
Xiangzun
X
Wang
Key Laboratory of Entomology and Pest Control Engineering of Chongqing, College of Plant Protection, Southwest University, Chongqing, China.
Guangmao
G
Shen
Key Laboratory of Entomology and Pest Control Engineering of Chongqing, College of Plant Protection, Southwest University, Chongqing, China.
Lin
L
He
D000818:Animals; D019295:Computational Biology; D020451:Contig Mapping; D030541:Databases, Genetic; D005260:Female; D020869:Gene Expression Profiling; D016678:Genome; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000737:chemistry*; D008297:Male; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature; D040102:Tetranychidae / Q000235:genetics*; D059467:Transcriptome
D007306:Insecticides; D012333:RNA, Messenger