Blood flow to follicles and CL during development of the periovulatory follicular wave in heifers.
The hemodynamics of the developing CL and the future dominant follicle (DF) was studied in 22 heifers during wave 1 on Days 0 to 5 (Day 0 = ovulation). Color-Doppler ultrasonography was used to determine the resistance index (RI) at the most prominent Doppler signal in an ovarian arterial branch before entry into the ovary; a decrease in RI indicates a downstream increase in vascular perfusion. The RI for each of four intraovarian patterns averaged over days was different (P < 0.05) from each of the other patterns as follows: DF-CL (DF and CL in the same ovary), 0.52 ± 0.02; CL alone, 0.60 ± 0.01; DF alone, 0.67 ± 0.01; neither DF nor CL, 0.78 ± 0.01. The differences in RI among intraovarian patterns began on Day 0 or 1, indicating that the extent of vascular perfusion on Days 0 to 5 for the various patterns may have been influenced by events that occurred before ovulation. The percentage of the DF wall with color-flow signals was greater (P < 0.05) in the DF-CL pattern than in the DF pattern on each of Days 2 to 5 and was greater (P < 0.0001) in the DF-CL pattern when the DF was adjacent to the CL (40.2 ± 2.0%) than when separated (24.5 ± 1.9%). Dimensions of DF (P < 0.01) and CL (P < 0.02) were greater when adjacent to each other. The results supported the hypotheses for wave 1 that (1) vascular perfusion is greater for the DF-CL intraovarian pattern than for the DF or CL pattern and (2) the extent of blood-flow Doppler signals in the wall of the developing DF is greater for the DF-CL pattern than for the DF pattern. Our preferred interpretation is that a change in vascular perfusion of the CL is accompanied by a similar change in perfusion of the DF when the two structures are in the same ovary especially adjacent.
Eutheria Foundation, Cross Plains, Wisconsin, USA; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA. Electronic address: ginther@vetmed.wisc.edu.
O J
OJ
Ginther
Eutheria Foundation, Cross Plains, Wisconsin, USA.
H B
HB
Rakesh
Eutheria Foundation, Cross Plains, Wisconsin, USA.
M M
MM
Hoffman
D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000098:blood supply* / Q000000981:diagnostic imaging; D005260:Female; D006439:Hemodynamics; D006080:Ovarian Follicle / Q000098:blood supply* / Q000000981:diagnostic imaging; D010060:Ovulation / Q000502:physiology*; D012039:Regional Blood Flow; D018615:Ultrasonography, Doppler, Color / Q000662:veterinary
Corpus luteum; Dominant follicle; Follicle deviation; Intraovarian pattern; Ultrasonography