Micellar electrokinetic chromatographic determination of rosuvastatin in rabbit plasma and evaluation of its pharmacokinetics and interaction with niacin.
A specific, accurate, precise and reproducible micellar electrokinetic chromatographic method was developed for in vitro and in vivo estimation of rosuvastatin, a synthetic and potent HMG-CoA inhibitor, in rabbit plasma. Further, its pharmacokinetics in the presence of niacin, which could be co-administered for monitoring of severe hypercholestremia, was investigated. The assay procedures involved simple liquid-liquid extraction of rosuvastatin and internal standard, atorvastatin, from a small plasma volume directly into acetonitrile. The organic layer was separated and evaporated under a gentle stream of nitrogen. The residue was reconstituted in the mobile phase and injected electrokinetically into electropherosis system. The background electrolyte consisted of borate buffer (25.0 mm, pH 9.5), 10.0% organic modifier (5.0% methanol + 5.0% acetonitrile) and 25.0 mm sodium dodecyl sulfate at 20.0 kV applied voltage and 215.0 nm detection wavelength for the effective separation of rosuvastatin, niacin and atorvastatin.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt.
Michael E
ME
El-Kommos
Niveen A
NA
Mohamed
Hassan R H
HR
Ali
Ahmed F
AF
Abdel Hakiem
D000818:Animals; D000069059:Atorvastatin; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004347:Drug Interactions; D005464:Fluorobenzenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D006538:Heptanoic Acids; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D009525:Niacin / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D011743:Pyrimidines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D011758:Pyrroles; D011817:Rabbits; D015203:Reproducibility of Results; D000068718:Rosuvastatin Calcium; D012967:Sodium Dodecyl Sulfate; D013449:Sulfonamides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*
D005464:Fluorobenzenes; D006538:Heptanoic Acids; D011743:Pyrimidines; D011758:Pyrroles; D013449:Sulfonamides; D009525:Niacin; D012967:Sodium Dodecyl Sulfate; D000068718:Rosuvastatin Calcium; D000069059:Atorvastatin
micellar electrokinetic chromatographic method; niacin; pharmacokinetic study; rabbit plasma; rosuvastatin