Monitoring of the action of drugs in melanoma cells by dynamic laser speckle.
This work presents the development of a protocol based on the dynamic laser speckle designed to monitor the reaction of cancer cells of line MEL-RC08 to the application of the drug Colcemid in two different concentrations: 0.2 and 0.4 μg/mL. The protocol was designed using the forward scattering approach with an He-Ne laser of 632.8 nm illuminating the samples, a control, and two variations of Colcemid, being monitored along 8 h. The data were analyzed numerically in the time and in the frequency domain, and the results presented the ability of the technique to monitor the action of the drug, particularly Colcemid (0.4 μg/mL).
Universidad de Valencia, Unidad de Biofísica y Física Médica, Departamento de Fisiología, Facultad de Medicina y Odontología, Valencia, Spain, CP 46010.
Rolando J
RJ
González-Peña
Universidade Federal de Lavras, Departamento de Engenharia, LavrasMG, Brazil, CP 3037.
Roberto A
RA
Braga
Universidad de Valencia, Unidad de Biofísica y Física Médica, Departamento de Fisiología, Facultad de Medicina y Odontología, Valencia, Spain, CP 46010.
Rosa M
RM
Cibrián
Universidad de Valencia, Unidad de Biofísica y Física Médica, Departamento de Fisiología, Facultad de Medicina y Odontología, Valencia, Spain, CP 46010.
Rosario
R
Salvador-Palmer
Universidad de Valencia, Departamento de Patología, Facultad de Medicina y Odontología, Valencia, Spain, CP 46010.
Rosario
R
Gil-Benso
Universidad de Valencia, Departamento de Patología, Facultad de Medicina y Odontología, Valencia, Spain, CP 46010.
Teresa San
TS
Miguel
D000704:Analysis of Variance; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D003703:Demecolcine / Q000494:pharmacology*; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007834:Lasers; D008545:Melanoma / Q000737:chemistry* / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D003703:Demecolcine