Isolation, structural elucidation and anti-inflammatory activity of astragalin, (-)hinokinin, aristolactam I and aristolochic acids (I & II) from Aristolochia indica.
Astragalin was isolated for the first time along with (-)hinokinin, aristolactam I and aristolochic acids (I & II) from the extracts of Aristolochia indica L. using a new, efficient preparative HPLC method. A reversed-phase HPLC method of analysis was developed to analyse the isolated compounds. The crude extracts and the isolated compounds were tested for their anti-inflammatory potential. We report here for the first time the anti-inflammatory effects of (-)hinokinin and aristolactam I against IL-6 (IC50 = 20.5 ± 0.5 and 52 ± 8 μM) and TNFα (IC50 = 77.5 ± 27.5 and 116.8 ± 83.25 μM), respectively. (-)Hinokinin exerted its anti-inflammatory effects via NFκB-dependent mechanism whereas aristolactam I may be effective via a mechanism independent of NFκB.
a Department of Medicinal Chemistry , Piramal Life Sciences, Piramal Enterprises Limited , Mumbai , Maharashtra , India.
Dattatraya C
DC
Desai
Jeenu
J
Jacob
Asha
A
Almeida
Rajendra
R
Kshirsagar
S L
SL
Manju
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000893:Anti-Inflammatory Agents; D030022:Aristolochia / Q000737:chemistry*; D034341:Aristolochic Acids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D052117:Benzodioxoles; D002851:Chromatography, High Pressure Liquid; D004149:Dioxoles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000093242:Interleukin-6 Inhibitors
D015107:4-Butyrolactone; D000893:Anti-Inflammatory Agents; D034341:Aristolochic Acids; D052117:Benzodioxoles; D004149:Dioxoles; D044949:Kaempferols; D017705:Lignans; D000093242:Interleukin-6 Inhibitors; C121135:aristolactam I; C475934:hinokinin; C001579:astragalin
( − )hinokinin; Aristolochia indica; IL-6; NFκB; TNFα; aristolactam I; astragalin