Prevalence of high-risk human papillomavirus by cobas 4800 HPV test in urban Peru.
BACKGROUND
Molecular tests allow the detection of high-risk human papillomavirus in cervical samples, playing an important role in the prevention of cervical cancer.


OBJECTIVE
We performed a study to determine the prevalence of HPV 16, HPV 18 and other high-risk human papillomavirus (pool 12 genotypes) in Peruvian females from diverse urban areas using the cobas 4800 HPV test.


METHODS
Routine cervical samples collected in our laboratory were analyzed by cobas 4800 HPV test.


RESULTS
A total of 2247 samples from female patients aged 17-79 years were tested. high-risk human papillomavirus was positive in 775 (34.49%) samples. Of these, 641 (82.71%) were single infections and 134 (17.29%) were multiple infections. The positivity rates for HPV 16, HPV 18, and other high-risk human papillomavirus were 10.77%, 2.0%, and 28.08%, respectively. In multiple high-risk human papillomavirus infections, the concomitance of HPV 16 and other high-risk human papillomavirus was more prevalent (13.42%).


CONCLUSIONS
Our study showed high prevalence of high-risk human papillomavirus in urban Peru, mainly among young women. In both single and multiple infections other high-risk human papillomavirus were more prevalent than HPV 16 and HPV 18, which might influence vaccine impact in our country. Furthermore, the cobas 4800 HPV test may be considered a useful tool for HPV molecular diagnosis.
Precisa Laboratorios Arias Stella, Lima, Peru. Electronic address: biomolecular@ariasstella.com.
Ricardo
R
Iwasaki
Precisa Laboratorios Arias Stella, Lima, Peru.
Felipe
F
Galvez-Philpott
Precisa Laboratorios Arias Stella, Lima, Peru.
Javier
J
Arias-Stella
Precisa Laboratorios Arias Stella, Lima, Peru.
Javier
J
Arias-Stella
D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D065006:Papanicolaou Test; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D010568:Peru / Q000453:epidemiology; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D014505:Urban Population; D014626:Vaginal Smears
D004279:DNA, Viral
Cobas 4800 HPV test; High-risk human papillomavirus; Prevalence