Peripheral blood miRNAs as a biomarker for chronic cardiovascular diseases.
Vascular diseases are the most prevalent diseases worldwide. This study intended to analyze peripheral blood miRNA levels and their correlation with NT-pro-BNP and cTN-I in patients with atherosclerosis or pre-atherosclerotic conditions to build a dynamic correlation between vascular diseases and their biomarkers. Serum NT-pro-BNP and cTN-I levels were measured by their respective ELISA kits. The miRNA levels were assayed by quantitative PCR. Unique miRNA signatures were identified for both atherosclerosis and pre-atherosclerosis. The levels of miR-92a, 126, 130a, 222, and 370 levels were decreased in the peripheral blood of pre-atherosclerotic subjects. In atherosclerosis, miR-21, 122, 130a, and 211 were significantly increased whereas miR-92a, 126, and 222 were markedly decreased. Serum levels of NT-pro-BNP and cTN-I correlated with each other and increased with the progression of atherosclerosis. Moreover, the levels of cTN-I and NT-pro-BNP were positively correlated with miR-21 and negatively correlated with miR-126. Integrating specific pattern of miRNA levels with NT-pro-BNP and/or cardiac troponin may improve the diagnosis of cardiovascular diseases.
Laboratory Medical College, Jilin Medical College, Jilin, China.
Yong
Y
Jiang
Laboratory Medical College, Jilin Medical College, Jilin, China.
Hui-yan
HY
Wang
Laboratory Medical College, Jilin Medical College, Jilin, China.
Yan
Y
Li
Su-hong
SH
Guo
Laboratory Medical College, Jilin Medical College, Jilin, China.
Lei
L
Zhang
Department of surgery, Jilin Medical College, Jilin, China.
Jian-hui
JH
Cai
School of Pharmacy, Jilin Medical College, Jilin, China.
Hong-mei
HM
Cao
School of Nursing, Jilin Medical College, Jilin, China.
Chun-yan
CY
Wang
Laboratory Medical College, Jilin Medical College, Jilin, China.
Hao
H
Wang
Laboratory Medical College, Jilin Medical College, Jilin, China.
Lei
L
Liu
D050197:Atherosclerosis / Q000097:blood* / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D002908:Chronic Disease; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis; D018450:Disease Progression; D005500:Follow-Up Studies; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000175:diagnosis; D006973:Hypertension / Q000097:blood / Q000175:diagnosis; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D020097:Natriuretic Peptide, Brain / Q000097:blood; D010446:Peptide Fragments / Q000097:blood; D011379:Prognosis; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D020107:Troponin T / Q000097:blood
D015415:Biomarkers; D035683:MicroRNAs; D010446:Peptide Fragments; D020107:Troponin T; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain