Isolation and functional characterization of DNA damage repair protein (DRT) from Lepidium latifolium L.
We have isolated and in silico characterized a cold regulated plastocyanin encoding gene from Lepidium latifolium L designated as LlaDRT. Its cDNA sequence (JN214346) consists of a 504 bp ORF, 48 and 205 bp of 5' and 3' UTR regions, respectively encoding a protein of 17.07 KDa and pI 4.95. In silico and phylogenetic analysis of LlaDRT suggested that the protein has features of a typical plastocyanin family member and of a nearest relative of the predominant isoform of Arabidopsis (PETE2) plastocyanin. Validation of stress response of LlaDRT by qPCR under different abiotic stress regulators viz salicylic acid, jasmonic acid, calcium chloride, ethylene and abscisic acid revealed its possible regulation and crosstalk amongst different pathways.
Defence Institute of Bio-Energy Research, Goraparao, PO Arjunpur, Haldwani 263139, India; Department of Biotechnology, Kumaun University, Bhimtal Campus, Uttarakhand 263136, India; University School of Biotechnology, Guru Gobind Singh Indraprastha University, Dwarka Sec. 16C, New Delhi 110078, India. Electronic address: vimlendusinha@gmail.com.
Vimlendu Bhushan
VB
Sinha
Defence Institute of Bio-Energy Research, Goraparao, PO Arjunpur, Haldwani 263139, India.
Atul
A
Grover
Defence Institute of Bio-Energy Research, Goraparao, PO Arjunpur, Haldwani 263139, India.
Zakwan
Z
Ahmed
Department of Biotechnology, Kumaun University, Bhimtal Campus, Uttarakhand 263136, India.
Veena
V
Pande
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D007438:Introns; D029686:Lepidium / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012639:Seeds / Q000737:chemistry; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics
D018076:DNA, Complementary
Arabidopsis; Cold stress proteins; Lepidium; Plastocyanin; Real-time polymerase chain reaction