Supercritical carbon dioxide extraction of capsaicinoids from malagueta pepper (Capsicum frutescens L.) assisted by ultrasound.
Extracts from malagueta pepper (Capsicum frutescens L.) were obtained using supercritical fluid extraction (SFE) assisted by ultrasound, with carbon dioxide as solvent at 15MPa and 40°C. The SFE global yield increased up to 77% when ultrasound waves were applied, and the best condition of ultrasound-assisted extraction was ultrasound power of 360W applied during 60min. Four capsaicinoids were identified in the extracts and quantified by high performance liquid chromatography. The use of ultrasonic waves did not influence significantly the capsaicinoid profiles and the phenolic content of the extracts. However, ultrasound has enhanced the SFE rate. A model based on the broken and intact cell concept was adequate to represent the extraction kinetics and estimate the mass transfer coefficients, which were increased with ultrasound. Images obtained by field emission scanning electron microscopy showed that the action of ultrasonic waves did not cause cracks on the cell wall surface. On the other hand, ultrasound promoted disturbances in the vegetable matrix, leading to the release of extractable material on the solid surface. The effects of ultrasound were more significant on SFE from larger solid particles.
College of Food Engineering, Food Engineering Department, University of Campinas, UNICAMP, 13083-862 Campinas, SP, Brazil.
Philipe
P
Santos
College of Food Engineering, Food Engineering Department, University of Campinas, UNICAMP, 13083-862 Campinas, SP, Brazil.
Ana C
AC
Aguiar
Department of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Campus de Excelencia Internacional Agroalimentario (CeiA3), P.O. Box 40, 11510 Puerto Real, Cádiz, Spain.
Gerardo F
GF
Barbero
Institute of Chemistry, University of Campinas, UNICAMP, 13083-862 Campinas, SP, Brazil.
Camila A
CA
Rezende
College of Food Engineering, Food Engineering Department, University of Campinas, UNICAMP, 13083-862 Campinas, SP, Brazil. Electronic address: julian@fea.unicamp.br.
Julian
J
Martínez
D002211:Capsaicin / Q000302:isolation & purification*; D002212:Capsicum / Q000737:chemistry*; D002245:Carbon Dioxide / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D007700:Kinetics; D008962:Models, Theoretical; D011312:Pressure; D014465:Ultrasonics
D002245:Carbon Dioxide; D002211:Capsaicin
Capsaicinoids; Mathematical modeling; Microscopy; Pepper; Supercritical extraction; Ultrasound