Synergistic effects of oleaginous yeast Rhodotorula glutinis and microalga Chlorella vulgaris for enhancement of biomass and lipid yields.
The optimal mixed culture model of oleaginous yeast Rhodotorula glutinis and microalga Chlorella vulgaris was confirmed to enhance lipid production. A double system bubble column photo-bioreactor was designed and used for demonstrating the relationship of yeast and alga in mixed culture. The results showed that using the log-phase cultures of yeast and alga as seeds for mixed culture, the improvements of biomass and lipid yields reached 17.3% and 70.9%, respectively, compared with those of monocultures. Growth curves of two species were confirmed in the double system bubble column photo-bioreactor, and the second growth of yeast was observed during 36-48 h of mixed culture. Synergistic effects of two species for cell growth and lipid accumulation were demonstrated on O2/CO2 balance, substance exchange, dissolved oxygen and pH adjustment in mixed culture. This study provided a theoretical basis and culture model for producing lipids by mixed culture in place of monoculture.
National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Zhiping
Z
Zhang
National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Hairui
H
Ji
National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Guiping
G
Gong
National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: zhangxu@mail.buct.edu.cn.
Xu
X
Zhang
National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Tianwei
T
Tan
D018533:Biomass; D002245:Carbon Dioxide / Q000032:analysis; D048408:Chlorella vulgaris / Q000254:growth & development / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000032:analysis; D010100:Oxygen / Q000032:analysis; D058433:Photobioreactors / Q000382:microbiology; D012248:Rhodotorula / Q000254:growth & development / Q000378:metabolism*; D012995:Solubility; D013997:Time Factors
D008055:Lipids; D002245:Carbon Dioxide; D009584:Nitrogen; D010100:Oxygen
Chlorella vulgaris; Lipid; Mixed culture; Rhodotorula glutinis; Synergistic effects