A highly reproducible mouse model of compression spinal cord injury.
Experimental spinal cord injury (SCI) can maintain the continuity of the spinal cord, as in the contusion (e.g., weight-fall) or compression models, or not, when there is a partial or a complete transection. The majority of acute human SCI is not followed by complete transection, but there is a combination of contusion, compression, and possibly partial transection. The method described here is a compressive mouse model that presents a combination of contusion and compression components and has many facilities in its execution. This lesion was established by our group and represents a simple, reliable, and inexpensive clip compression model with functional and morphological reproducibility. In this chapter we describe, step by step, the protocol of this experimental SCI.
Laboratório de Regeneração Neural e Função, Departamento de Neurobiologia, Fluminense Federal University, Niterói, RJ, Brazil.
Suelen Adriani
SA
Marques
Fernanda Martins
FM
de Almeida
Klauss
K
Mostacada
Ana Maria Blanco
AM
Martinez
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D019635:Neurosurgical Procedures / Q000379:methods; D013116:Spinal Cord / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D013117:Spinal Cord Compression / Q000473:pathology / Q000503:physiopathology