Enhancement of 1,3-propanediol production by expression of pyruvate decarboxylase and aldehyde dehydrogenase from Zymomonas mobilis in the acetolactate-synthase-deficient mutant of Klebsiella pneumoniae.
The acetolactate synthase (als)-deficient mutant of Klebsiella pneumoniae fails to produce 1,3-propanediol (1,3-PD) or 2,3-butanediol (2,3-BD), and is defective in glycerol metabolism. In an effort to recover production of the industrially valuable 1,3-PD, we introduced the Zymomonas mobilis pyruvate decarboxylase (pdc) and aldehyde dehydrogenase (aldB) genes into the als-deficient mutant to activate the conversion of pyruvate to ethanol. Heterologous expression of pdc and aldB efficiently recovered glycerol metabolism in the 2,3-BD synthesis-defective mutant, enhancing the production of 1,3-PD by preventing the accumulation of pyruvate. Production of 1,3-PD in the pdc- and aldB-expressing als-deficient mutant was further enhanced by increasing the aeration rate. This system uses metabolic engineering to produce 1,3-PD while minimizing the generation of 2,3-BD, offering a breakthrough for the industrial production of 1,3-PD from crude glycerol.
Biofinery Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk, 580-185, South Korea.
Sung-Mok
SM
Lee
Won-Kyung
WK
Hong
Sun-Yeon
SY
Heo
Jang Min
JM
Park
You Ree
YR
Jung
Baek-Rock
BR
Oh
Min-Ho
MH
Joe
Jeong-Woo
JW
Seo
Chul Ho
CH
Kim
D000095:Acetolactate Synthase / Q000172:deficiency; D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D019149:Bioreactors; D053898:Biosynthetic Pathways / Q000502:physiology*; D000431:Ethanol / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D060847:Metabolic Engineering / Q000379:methods; D011409:Propylene Glycols / Q000378:metabolism*; D011767:Pyruvate Decarboxylase / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D017355:Zymomonas / Q000201:enzymology*
D011409:Propylene Glycols; D000431:Ethanol; C041787:1,3-propanediol; D019289:Pyruvic Acid; D000444:Aldehyde Dehydrogenase; D000095:Acetolactate Synthase; D011767:Pyruvate Decarboxylase; D005990:Glycerol