Identification of serum microRNAs as diagnostic and prognostic biomarkers for acute pancreatitis.
OBJECTIVE
To identify serum microRNA (miRNA) as diagnostic and prognostic biomarkers for acute pancreatitis (AP).


METHODS
Sera microRNA expression was profiled from 12 AP patients with varying disease severity and three healthy controls. Differentially expressed miRNAs were validated in a larger cohort of patients and controls. The diagnostic and prognostic potentials of differentially expressed miRNAs were evaluated using receiver operating characteristic (ROC) curve analysis and compared to that of classic prognostic markers for AP.


RESULTS
miRNA microarray analyses identified 205 differentially expressed miRNAs between sera from AP patients and that from controls. Nine miRNAs were differentially expressed between severe and mild AP patients. Further validation confirmed the down-regulation of miR-92b, miR-10a, and miR-7 in AP patients, and ROC analysis revealed that these miRNAs can differentiate AP from health cases. Furthermore, the serum miR-551b-5p level was significantly higher in patients with disease complications or a low plasma calcium level. ROC analysis showed that the serum miR-551b-5p level can distinguish between severe and mild AP.


CONCLUSIONS
The expressions of miR-92b, miR-10a, and miR-7 in AP might be used for the early diagnosis of AP and miR-551b-5p may be used for predicting AP severity.
Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: liupi1974@sina.com.
Pi
P
Liu
Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Liang
L
Xia
Nanchang University, Nanchang 330031, China.
Wei-Long
WL
Zhang
Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Hua-Jing
HJ
Ke
Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Tao
T
Su
Nanchang University, Nanchang 330031, China.
Li-Bing
LB
Deng
Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
You-Xiang
YX
Chen
Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: lunonghua@163.com.
Nong-Hua
NH
Lv
D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010195:Pancreatitis / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D011379:Prognosis; D012372:ROC Curve; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index
D015415:Biomarkers; D035683:MicroRNAs
Acute pancreatitis; Biomarker; Early diagnosis; Prediction; Prognosis; microRNA