A coumarin-quinolinium-based fluorescent probe for ratiometric sensing of sulfite in living cells.
Based on a novel coumarin-quinolinium platform, probe 2 was rationally designed and synthesized as a novel ratiometric fluorescent sensor for sulfite anions. The probe exhibited a wide dynamic concentration range for sulfite anions in a PBS buffer (containing 1 mg mL(-1) BSA). More importantly, the probe was suitable for ratiometric fluorescence imaging in living cells with high sensitivity, favorable selectivity, and minimal cytotoxicity.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, P. R. China. weiyinglin2013@163.com.
Li
L
Tan
Weiying
W
Lin
Sasa
S
Zhu
Lin
L
Yuan
Kaibo
K
Zheng
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002470:Cell Survival; D003374:Coumarins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008958:Models, Molecular; D066244:Proton Magnetic Resonance Spectroscopy; D011789:Quantum Theory; D011806:Quinolinium Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013447:Sulfites / Q000032:analysis*; D013997:Time Factors
D003374:Coumarins; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; D013447:Sulfites; C030123:coumarin