In vivo optical imaging of brain tumors and arthritis using fluorescent SapC-DOPS nanovesicles.
We describe a multi-angle rotational optical imaging (MAROI) system for in vivo monitoring of physiopathological processes labeled with a fluorescent marker. Mouse models (brain tumor and arthritis) were used to evaluate the usefulness of this method. Saposin C (SapC)-dioleoylphosphatidylserine (DOPS) nanovesicles tagged with CellVue Maroon (CVM) fluorophore were administered intravenously. Animals were then placed in the rotational holder (MARS) of the in vivo imaging system. Images were acquired in 10° steps over 380°. A rectangular region of interest (ROI) was placed across the full image width at the model disease site. Within the ROI, and for every image, mean fluorescence intensity was computed after background subtraction. In the mouse models studied, the labeled nanovesicles were taken up in both the orthotopic and transgenic brain tumors, and in the arthritic sites (toes and ankles). Curve analysis of the multi angle image ROIs determined the angle with the highest signal. Thus, the optimal angle for imaging each disease site was characterized. The MAROI method applied to imaging of fluorescent compounds is a noninvasive, economical, and precise tool for in vivo quantitative analysis of the disease states in the described mouse models.
Division of Hematology-Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine; Division of Human Genetics, University of Cincinnati College of Medicine.
Zhengtao
Z
Chu
Department of Radiology, University of Cincinnati College of Medicine.
Kathleen
K
LaSance
Division of Hematology-Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine.
Victor
V
Blanco
Solid Tumor Biology Program, James Comprehensive Cancer Center, The Ohio State University Medical Center; Department of Neurological Surgery, James Comprehensive Cancer Center, The Ohio State University Medical Center.
Chang-Hyuk
CH
Kwon
Solid Tumor Biology Program, James Comprehensive Cancer Center, The Ohio State University Medical Center; Department of Neurological Surgery, James Comprehensive Cancer Center, The Ohio State University Medical Center.
Balveen
B
Kaur
Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine.
Malinda
M
Frederick
Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine.
Sherry
S
Thornton
Department of Radiology, University of Cincinnati College of Medicine.
Lisa
L
Lemen
Division of Hematology-Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine; Division of Human Genetics, University of Cincinnati College of Medicine; xiaoyang.qi@uc.edu.
Xiaoyang
X
Qi
D000042:Absorption; D000818:Animals; D001168:Arthritis / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D005260:Female; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D008819:Mice, Nude; D008822:Mice, Transgenic; D049329:Nanostructures / Q000008:administration & dosage*; D061848:Optical Imaging; D055095:Optics and Photonics / Q000295:instrumentation / Q000379:methods*; D010718:Phosphatidylserines / Q000008:administration & dosage*; D049231:Saposins / Q000008:administration & dosage*; D051598:Whole Body Imaging
D005456:Fluorescent Dyes; D010718:Phosphatidylserines; D049231:Saposins; C042511:1,2-dioleoylphosphatidylserine