IL-6(-572/-597) polymorphism and expression in HBV disease chronicity in an Indian population.
OBJECTIVE
This study evaluated the association among IL-6(-572) and IL-6(-597) genotypes, haplotypes, mRNA, and protein levels with hepatitis B virus (HBV)-Hepatocellular carcinoma (HCC) risk in India.


METHODS
For this, 403 participants (153 controls, 61 inactive HBV-carriers, 65 chronic-active HBV patients, 63 HBV-cirrhotics, and 61 HBV-HCC participants) were enrolled in the study. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), ELISA, and RT-PCR methods were used for assessing polymorphism, protein, and the mRNA levels, respectively, of IL-6.


RESULTS
The study revealed that the IL-6(-572) GC genotype shared a positive association with hepatitis among controls, and a negative association with cirrhosis and consequent HCC development among carriers. However, the CC genotype shared a significant negative association with cirrhosis among controls and carriers. The IL-6(-597G>A), GA genotype acted as a potential protective factor for hepatitis, cirrhosis, and subsequent HCC development among carriers. The GA and CG haplotypes acted as a vital risk factor for HCC among controls and carriers. On the contrary, the CA haplotype was found to be a potential protective factor for HCC among carriers. Besides, the IL-6 levels significantly increased with cirrhosis development, as compared to carriers and hepatitis subjects.


CONCLUSIONS
These preliminary findings indicate a potential role of IL-6(-572/-597) genotypes in HBV disease pathogenesis in an Indian population.
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, 160 012, India.
Roli
R
Saxena
Yogesh Kumar
YK
Chawla
Indu
I
Verma
Jyotdeep
J
Kaur
D006528:Carcinoma, Hepatocellular / Q000453:epidemiology / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D005838:Genotype; D006515:Hepatitis B virus / Q000502:physiology; D019694:Hepatitis B, Chronic / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D008113:Liver Neoplasms / Q000453:epidemiology / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012307:Risk Factors
C508600:IL6 protein, human; D015850:Interleukin-6; D012333:RNA, Messenger