Bioanalytical approaches for the detection of single nucleotide polymorphisms by Surface Plasmon Resonance biosensors.
The mapping of specific single nucleotide polymorphisms (SNPs) in patients' genome is a main goal in theranostics, aiming to the development of therapies based on personalized medicine. In this review, Surface Plasmon Resonance (SPR) and Surface Plasmon Resonance imaging (SPRi) biosensors applied to the recognition of SNPs were reviewed, since these technologies are emerging in clinical diagnosis as powerful tools thanks to their analytical features, mainly the real-time and label-free monitoring based on array format for parallel analysis. Since the literature is heterogeneous, a critical classification and a systemic comparison of the analytical performances of published methods were here reviewed on the basis of the analytical strategy and the assay design. In particular, the use of helping agents (i.e. proteins, nanoparticles (NPs), intercalating agents) or artificial DNAs, often coupled to SPR to achieve allele discrimination and/or enhanced sensitivity, were here revised and classified. Finally, the real suitability of SPR biosensors to clinical diagnostics for SNPs detection was addressed by comparing their features and performances with those of other biosensors based on other techniques (e.g. electrochemical biosensors).
Dipartimento di Chimica "Ugo Schiff", Università di Firenze, via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy.
Maria Laura
ML
Ermini
Dipartimento di Chimica "Ugo Schiff", Università di Firenze, via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy.
Stefano
S
Mariani
Dipartimento di Chimica "Ugo Schiff", Università di Firenze, via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy.
Simona
S
Scarano
Dipartimento di Chimica "Ugo Schiff", Università di Firenze, via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy; Consorzio Sistemi a Grande Interfase, Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy. Electronic address: maria.minunni@unifi.it.
Maria
M
Minunni
D000818:Animals; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006801:Humans; D007364:Intercalating Agents / Q000737:chemistry; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
D007364:Intercalating Agents
Clinical diagnostics; Nanoparticles (NPs); Optical biosensors; Single nucleotide polymorphisms (SNPs); Surface Plasmon Resonance (SPR); Surface Plasmon Resonance imaging (SPRi)