Enzymatic cocktails produced by Fusarium graminearum under submerged fermentation using different lignocellulosic biomasses.
Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and type of proteins secreted. The cell wall-degrading enzymes (CWDE) proportion varied greatly from 20% to 69%. Only one of the 57 CWDEs detected in this study was common to the five proteomes. In contrast, 35 CWDEs were specific to one proteome only. The polysaccharide-degradation activities were different depending on the cocktail and the polysaccharide used. F. graminearum strongly modifies the enzymatic cocktail it secretes as a function of the biomass used for growth.
Institut de Chimie et Procédés pour l'Énergie, l'Environnement et la Santé (UMR 7515 Strasbourg University/CNRS), ECPM, Strasbourg Cedex 2, France.
Philippe
P
Debeire
Francois
F
Delalande
Olivier
O
Habrylo
Jean-Marc
JM
Jeltsch
Alain
A
Van Dorsselaer
Vincent
V
Phalip
D018533:Biomass; D005285:Fermentation; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005947:Glucose / Q000737:chemistry; D008031:Lignin / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D011134:Polysaccharides; D020543:Proteome; C036909:lignocellulose; D008031:Lignin; D005947:Glucose
CWDE; GH61; biofuels; glycosyl hydrolases; proteomics