Detection of Helicobacter pylori in adenoid tissue by real-time polymerase chain reaction.
OBJECTIVE
This study aims to investigate the presence of Helicobacter pylori (H. pylori) using the real-time polymerase chain reaction (PCR) method in the adenoid tissues in children undergoing surgical operation due to adenoid hypertrophy.


METHODS
Adenoid tissues of 23 children (8 girls, 15 boys; mean age 6.2 years; range 3 to 9 years) who were operated with the diagnosis of adenoid hypertrophy, tonsil + adenoid hypertrophy or tonsil + adenoid hypertrophy + serous otitis media in our clinic between January 2012 and April 2012 were examined.


RESULTS
Of 23 patients, H. pylori was detected in the adenoid tissues of two (8.7%). Regurgitation was present in seven patients. However, no regurgitation was found in H. pylori-positive patients.


CONCLUSIONS
In this study the presence of H. pylori in patients with adenoid hypertrophy has been demonstrated using PCR method. To be able to support the hypothesis that H. pylori has a place in etiology of adenoid hypertrophy, multicenter studies are warranted.
Department of Otolaryngology, Medical Faculty of Balıkesir University, 35100 Balıkesir, Turkey.
Mustafa
M
Kaymakçı
Mehtap
M
Aydın
Selçuk
S
Yazıcı
Ozlem
O
Sağır
Ozer Erdem
OE
Gür
Murat
M
Sayan
D000234:Adenoids / Q000382:microbiology / Q000473:pathology* / Q000601:surgery; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D016481:Helicobacter Infections; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D006984:Hypertrophy / Q000150:complications / Q000382:microbiology / Q000473:pathology* / Q000601:surgery; D008297:Male; D010034:Otitis Media with Effusion / Q000150:complications*; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction
D004269:DNA, Bacterial