The effect of epigallocatechin gallate on hepatocytes isolated from normal and partially hepatectomized rats.
Epigallocatechin gallate (EGCG) is an antioxidant found in green tea. In this study, male Wistar rats were subjected either to partial hepatectomy (PHx), or a sham operation (LAP). Twenty-four hours after surgery, hepatocytes were isolated and treated with various concentrations of EGCG for up to 72 h. We then measured markers of cell viability, oxidative stress, DNA synthesis, and caspase activity. Morphological criteria, cell viability tests, and albumin synthesis revealed toxicity starting at 10 μmol/L. DNA synthesis was higher in hepatocytes isolated from rats after PHx and inhibited by EGCG. Furthermore, EGCG increased the activity of caspases 3 and 7, seen more in hepatocytes from PHx rats. In conclusion, EGCG at a concentration of 10 μmol/L was toxic for hepatocytes isolated from both PHx and LAP rats.
a Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University in Prague, Simkova 870, 500 38 Hradec Králové 1, Czech Republic.
Vojtech
V
Mezera
Otto
O
Kucera
Alena
A
Moravcova
Eva
E
Peterova
Zuzana
Z
Cervinkova
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000633:toxicity*; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006498:Hepatectomy; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D002392:Catechin; D004247:DNA; C045651:epigallocatechin gallate; D053148:Caspase 3; D053179:Caspase 7
antioxidants; antioxydants; apoptose; apoptosis; catechin; catéchine; culture cellulaire primaire; epigallocatechin gallate; hepatectomy; hepatocytes; hépatectomie; hépatocytes; le gallate d’épigallocatéchine; liver regeneration; oxidative stress; primary cell culture; régénération du foie; stress oxydatif