Distribution of nucleosides in populations of Cordyceps cicadae.
A rapid HPLC method had been developed and used for the simultaneous determination of 10 nucleosides (uracil, uridine, 2'-deoxyuridine, inosine, guanosine, thymidine, adenine, adenosine, 2'-deoxyadenosine and cordycepin) in 10 populations of Cordyceps cicadae, in order to compare four populations of Ophicordyceps sinensis and one population of Cordyceps militaris. Statistical analysis system (SAS) 8.1 was used to analyze the nucleoside data. The pattern of nucleoside distribution was analyzed in the sampled populations of C. cicadae, O. sinensis and C. militaris, using descriptive statistical analysis, nested analysis and Q cluster analysis. The total amount of the 10 nucleosides in coremium was 1,463.89-5,678.21 µg/g in 10 populations of C. cicadae, 1,369.80-3,941.64 µg/g in sclerotium. The average contents of the 10 analytes were 4,392.37 µg/g and 3,016.06 µg/g in coremium and sclerotium, respectively. The coefficient of variation (CV) of nucleosides ranged from 8.36% to 112.36% in coremium of C. cicadae, and from 10.77% to 155.87% in sclerotium of C. cicadae. The CV of the nucleosides was wide within C. cicadae populations. The nested variation analysis by the nine nucleosides' distribution indicated that about 42.29% of the nucleoside variability in coremium was attributable to the differentiation among populations, and the remaining 57.71% resided in the populations. It was also shown that about 28.94% of the variation in sclerotium was expressed between populations, while most of the variation (71.06%) corresponded to the populations.
Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, Yunnan, China. zengwenboherb@163.com.
Wen-Bo
WB
Zeng
Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, Yunnan, China. herbfish@163.com.
Hong
H
Yu
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan, China. gefeng79@aliyun.com.
Feng
F
Ge
Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, Yunnan, China. icecream6973@sina.com.
Jun-Yuan
JY
Yang
Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, Yunnan, China. czh78@tom.com.
Zi-Hong
ZH
Chen
Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, Yunnan, China. wangyb001@126.com.
Yuan-Bing
YB
Wang
Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, Yunnan, China. daidiy555@gmail.com.
Yong-Dong
YD
Dai
Department of Biological Sciences, College of Engineering, Forestry and Natural Science, Northern Arizona University, Flagstaff, AZ 86011-5640, USA. Alison.Adams@nau.edu.
Alison
A
Adams
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D032661:Cordyceps / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D011153:Population / Q000235:genetics*; D013045:Species Specificity
D009705:Nucleosides