Does the relief of glucose toxicity act as a mediator in proliferative actions of vanadium on pancreatic islet beta cells in streptozocin diabetic rats?
Data shows vanadium protects pancreatic beta cells (BC) from diabetic animals. Whether this effect is direct or through the relief of glucose toxicity is not clear. This study evaluated the potential effect of oral vanadyl sulfate (vanadium) on glycemic status and pancreatic BC of normal and diabetic rats.



Rats were divided into five groups of normal and diabetic. Diabetes was induced with streptozocin (40 mg/kg, i.v.). Normal rats used water (CN) or vanadium (1 mg/ml VOSO4, VTN). Diabetic rats used water (CD), water plus daily neutral protamine Hagedorn insulin injection (80 U/kg, ITD) or vanadium (VTD). Blood samples were taken for blood glucose (BG, mg/dL) and insulin (ng/dL) measurements. After two months, the pancreata of sacrificed rats were prepared for islet staining.



Pre-treated normal BG was 88 ± 2, and diabetic BG was 395 ± 9. The final BG in CD, VTD, and ITD was 509 ± 22, 138 ± 14, and 141 ± 14, respectively. Insulin in VTN (0.75 ± 0.01) and VTD (0.78 ± 0.01) was similar, higher than CD (0.51 ± 0.07) but lower than CN (2.51 ± 0.02). VTN islets compared to CN had larger size and denser central core insulin immunoreactivity with plentiful BC. CD and ITD islets were atrophied and had scattered insulin immunoreactivity spots and low BC mass. VTD islets were almost similar to CN.



Besides insulin-like activity, vanadium protected pancreatic islet BC, and the relief of glucose toxicity happening with vanadium had a little role in this action.
Dept. of Physiology, Nemazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
Leila
L
Pirmoradi
Dept. of Physiology, Nemazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
Mohammad Taghi
MT
Mohammadi
Dept. of Pathology, Nemazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
Akbar
A
Safaei
Dept. of Anatomy, Nemazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
Fakhardin
F
Mesbah
Dept. of Physiology, Nemazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.|Dept. of Pathology, Nemazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
Gholam Abbas
GA
Dehghani
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000473:pathology*; D004327:Drinking Behavior / Q000187:drug effects; D005947:Glucose / Q000633:toxicity*; D007150:Immunohistochemistry; D007328:Insulin / Q000097:blood; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000473:pathology*; D008297:Male; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D014639:Vanadium / Q000494:pharmacology*
D001786:Blood Glucose; D007328:Insulin; D014639:Vanadium; D013311:Streptozocin; D005947:Glucose
Vanadium; Rats; Diabetes; Protection; Beta cells