NF-κB inhibitory activity of polyoxygenated steroids from the Vietnamese soft coral Sarcophyton pauciplicatum.
Chromatographic purification of the methanolic extract from the soft coral Sarcophyton pauciplicatum led to the isolation of three polyhydroxylated steroids 1-3, including a new compound, sarcopanol A (1). Their structures were elucidated by spectroscopic analysis and by comparison of the spectroscopic data with those of similar compounds previously reported in literature. The anti-inflammatory effects of isolated compounds were evaluated using nuclear factor kappa B (NF-κB) luciferase and reverse transcription polymerase chain reaction (RT-PCR). The effect of isolated compounds on cell growth was evaluated by MTS assays. Compounds 1 and 2 significantly inhibited TNFα/INFγ-induced NF-κB transcriptional activity in human keratinocyte (HaCaT) cells in a dose-dependent manner, with EC50 values of 8.27±3.28 and 26.07±5.59 μM, respectively. Furthermore, the transcriptional inhibition of these compounds was confirmed by a decrease in cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and intercellular adhesion molecule-1 (ICAM-1) gene expression levels in HaCaT cells.
Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam; College of Pharmacy, Chungnam National University, Daejeon 305-64, Republic of Korea.
Nguyen Phuong
NP
Thao
Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam; College of Pharmacy, Chungnam National University, Daejeon 305-64, Republic of Korea.
Bui Thi Thuy
BT
Luyen
College of Pharmacy, Chungnam National University, Daejeon 305-64, Republic of Korea.
Ya Nan
YN
Sun
College of Pharmacy, Chungnam National University, Daejeon 305-64, Republic of Korea; Gyeongbuk Institute for Bio-Industry (GIB), Andong-City, Gyeongbuk 760-380, Republic of Korea.
Seok Bean
SB
Song
Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
Nguyen Van
NV
Thanh
Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
Nguyen Xuan
NX
Cuong
Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
Nguyen Hoai
NH
Nam
Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
Phan Van
PV
Kiem
College of Pharmacy, Chungnam National University, Daejeon 305-64, Republic of Korea. Electronic address: yhk@cnu.ac.kr.
Young Ho
YH
Kim
Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam. Electronic address: cvminh@vast.ac.vn.
Chau Van
CV
Minh
D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006900:Hydroxylation; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D016328:NF-kappa B; D013256:Steroids; D018799:Intercellular Adhesion Molecule-1; D051546:Cyclooxygenase 2
Anti-inflammatory; NF-κB; Polyhydroxylated steroid; Sarcopanol A; Sarcophyton pauciplicatum; Soft coral