Transcervical embryo transfer in mice.
Although efficient transcervical transfer of embryos in mice would provide many advantages over a surgical method, the low success rate of transcervical transfer has hampered its acceptance and use. Here, we describe a novel device and protocol for transcervical embryo transfer in mice. Blastocysts from CD1 female mice were transferred into the uteri of 2.5-d pseudopregnant CD1 mice by using this method resulted in the successful development of 66.7% to 73.5% of the transferred blastocysts into live-born fetuses. Our method is as efficient as surgical embryo transfer yet is much simpler, easier, and markedly less traumatic to the recipient. In addition, our method provides hygienic and economic advantages and conforms to the principles of humane experimental technique. More importantly, our method provides a model for studying transcervical embryo transfer in cattle, other large animals, and humans.
College of Animal Science and Technology, Hebei University, Baoding, China.
Lifang
L
Cui
College of Animal Science and Technology, Hebei University, Baoding, China.
Zhennan
Z
Zhang
College of Animal Science and Technology, Hebei University, Baoding, China.
Fangyuan
F
Sun
College of Animal Science and Technology, Hebei University, Baoding, China.
Xinchong
X
Duan
School Hospital of Agricultural University of Hebei, Hebei University, Baoding, China.
Mianjuan
M
Wang
Centre of Laboratory Animal, Faculty of Medicine, Hebei University, Baoding, China.
Keqian
K
Di
College of Animal Science and Technology, Hebei University, Baoding, China. Lxyun@hebau.edu.cn.
Xiangyun
X
Li
D000818:Animals; D004624:Embryo Transfer / Q000191:economics / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D005260:Female; D051379:Mice; D014599:Uterus