Caveolin-1 plays a key role in the oleanolic acid-induced apoptosis of HL-60 cells.
Our previous study found that caveolin-1 (CAV-1) protein expression is upregulated during oleanolic acid (OA)-induced inhibition of proliferation and promotion of apoptosis in HL-60 cells. CAV-1 is the main structural protein component of caveolae, playing important roles in tumorigenesis and tumor development. It has been shown that cav-1 expression is lower in leukemia cancer cell lines SUP-B15, HL-60, THP-1 and K562 and in chronic lymphocytic leukemia primary (CLP) cells when compared with normal white blood cells, with the lowest cav-1 expression level found in HL-60 cells. To study the effects of cav-1 in HL-60 cells and the effects of cav-1 overexpression on OA drug efficacy, cav-1 was overexpressed in HL-60 cells using lentiviral-mediated transfection combined with OA treatment. The results showed that cav-1 overexpression inhibited HL-60 cell proliferation, promoted apoptosis, arrested the cell cycle in the G1 phase and inhibited activation of the PI3K/AKT/mTOR signaling pathway. Overexpression of CAV-1 also increased HL-60 cell sensitivity to OA. To further verify whether OA affects HL-60 cells via the activation of downstream signaling pathways by CAV-1, cav-1 gene expression was silenced using RNAi, and the cells were treated with OA to examine its efficacy. The results showed that after cav-1 silencing, OA had little effect on cell activity, apoptosis, the cell cycle and phosphorylation of HL-60 cells. This study is the first to show that CAV-1 plays a crucial role in the effects of OA on HL-60 cells.
School of Basic Medicine, Jiamusi University, Jiamusi 154000, P.R. China.
Wei
W
Ma
School of Basic Medicine, Jiamusi University, Jiamusi 154000, P.R. China.
Di-Di
DD
Wang
School of Basic Medicine, Jiamusi University, Jiamusi 154000, P.R. China.
Li
L
Li
Department of Biology, Mudanjiang Medical University, Mudanjiang 157011, P.R. China.
Yu-Kuan
YK
Feng
Department of Biology, Mudanjiang Medical University, Mudanjiang 157011, P.R. China.
Hong-Mei
HM
Gu
School of Basic Medicine, Jiamusi University, Jiamusi 154000, P.R. China.
Gui-Ming
GM
Zhu
School of Basic Medicine, Jiamusi University, Jiamusi 154000, P.R. China.
Jin-Hua
JH
Piao
School of Basic Medicine, Jiamusi University, Jiamusi 154000, P.R. China.
Yu
Y
Yang
Department of Biochemistry, Harbin Medical University, Harbin 150086, P.R. China.
Xu
X
Gao
School of Basic Medicine, Jiamusi University, Jiamusi 154000, P.R. China.
Peng-Xia
PX
Zhang
D017209:Apoptosis / Q000187:drug effects; D051242:Caveolin 1 / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D020868:Gene Silencing; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D009828:Oleanolic Acid / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects
D051242:Caveolin 1; D009828:Oleanolic Acid