The effects of dietary xylooligosaccharide on mucosal parameters, intestinal microbiota and morphology and growth performance of Caspian white fish (Rutilus frisii kutum) fry.
The present study investigates the effects of different levels of dietary xylooligosaccharide (XOS) on skin mucus bactericidal activity and protein content, intestinal microbiota and morphology as well as growth performance of Caspian white fish (Rutilus frisii kutum) fry. The specimens (1.54 ± 0.03 g) were fed experimental diets supplemented with different levels of XOS [0% (control), 1%, 2% and 3%] for 8 weeks. The results revealed that feeding on XOS significantly increased skin mucus antibacterial activity and protein levels (P < 0.05) and the highest levels were observed in 3% XOS treatment. The total autochthonous intestinal heterotrophic bacteria significantly increased following XOS administration in diet (P < 0.05). Administration of 2 or 3% XOS in fry diet significantly elevated the autochthonous lactic acid bacteria (P < 0.05). However, dietary XOS had no significant effect on intestinal morphology, growth performance and diet utilisation of Caspian white fish fry (P > 0.05). These results demonstrate beneficial effects of dietary XOS on different parameters of mucosal immunity (both skin mucus and intestinal microbiota) and encourage further studies regards various aspect of XOS administration in early life stages of fish.
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: hoseinifar@gau.ac.ir.
Seyed Hossein
SH
Hoseinifar
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Maryam
M
Sharifian
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Mohammad Javad
MJ
Vesaghi
Department of Fisheries, Faculty of Agriculture and Natural Resource, Gonbad Kavous University, Gonbad, Iran.
Mohsen
M
Khalili
Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
M Ángeles
MÁ
Esteban
D000704:Analysis of Variance; D000818:Animals; D017756:Aquaculture / Q000379:methods; D003530:Cyprinidae / Q000254:growth & development* / Q000276:immunology*; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000382:microbiology*; D005965:Glucuronates / Q000494:pharmacology*; D018928:Immunity, Mucosal / Q000187:drug effects*; D064307:Microbiota / Q000187:drug effects*; D009092:Mucous Membrane / Q000187:drug effects / Q000276:immunology; D009844:Oligosaccharides / Q000494:pharmacology*; D056692:Prebiotics; D012867:Skin / Q000187:drug effects / Q000276:immunology
D005965:Glucuronates; D009844:Oligosaccharides; D056692:Prebiotics; C570991:xylooligosaccharide
Caspian white fish (Rutilus rutilus); Intestinal microbiota; Mucosal parameters; Prebiotic; Xylooligosaccharide (XOS)