Lipase-catalyzed hydrolysis of linseed oil: optimization using response surface methodology.
Lipase-catalyzed hydrolysis of linseed oil was investigated. Four commercially available microbial lipases of Lipase AY, Lipozyme RMIM, Lipozyme TLIM, and Novozym 435 were used. Among these tested lipases, Lipase AY exhibited the best hydrolysis effeciency to linseed oil. The effect of reaction variables was also evaluated and optimized using response surface methodology. A second-order regression for the Box-Behken design was used to study the effect of five independent variables, such as, temperature, pH, oil-aqueous phase ratio, enzyme load, and reaction time, on the hydrolysis of linseed oil. The optimal conditions were as follows: temperature 33°C, pH 5.80, oil-aqueous phase ratio 0.90 (w/w), enzyme load 1.20% (relative to the weight of total substrates), and reaction time 3.33 h. Under these conditions, the hydrolysis ratio of linseed oil was 93.92±0.54%.
Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology.
Weiwei
W
Chen
Shangde
S
Sun
Shaohua
S
Liang
Le
L
Peng
Yadong
Y
Wang
Mi
M
Shen
D055162:Biocatalysis; D002627:Chemistry, Physical / Q000379:methods*; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008043:Linseed Oil / Q000737:chemistry* / Q000378:metabolism; D008049:Lipase / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D014867:Water
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D014867:Water; D008043:Linseed Oil; C093666:Lipozyme; C093667:Novozyme 435; D008049:Lipase