Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
Upon hydration, flax seeds secrete mucilages whose content and physico-chemical properties vary according to the genotype and environment. The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area. The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity). Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed. Seven fibre lines had mucilages particularly rich in galactose. High to very high variability was found for 14 traits. Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties. Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
Department of Oil and Fiber Crops, N.I.Vavilov Research Institute of Plant Industry, 190000, B. Morskaya, 42, Saint-Petersburg, Russia. Electronic address: a.pavlov@vir.nw.ru.
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Pavlov
University of Rouen, Chemistry Laboratory PBS, UMR 6270, CNRS, FR 3038, 76821 Mont-Saint-Aignan, France. Electronic address: Florence.paynel@univ-rouen.fr.
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Paynel
University of Rouen, Chemistry Laboratory PBS, UMR 6270, CNRS, FR 3038, 76821 Mont-Saint-Aignan, France. Electronic address: christophe.rihouey@univ-rouen.fr.
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Rihouey
Department of Oil and Fiber Crops, N.I.Vavilov Research Institute of Plant Industry, 190000, B. Morskaya, 42, Saint-Petersburg, Russia. Electronic address: e.porohovinova@gmail.com.
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Porokhovinova
Department of Oil and Fiber Crops, N.I.Vavilov Research Institute of Plant Industry, 190000, B. Morskaya, 42, Saint-Petersburg, Russia. Electronic address: n.brutch@vir.nw.ru.
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Brutch
University of Rouen, Chemistry Laboratory PBS, UMR 6270, CNRS, FR 3038, 76821 Mont-Saint-Aignan, France. Electronic address: claudine.morvan@univ-rouen.fr.
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Morvan
D002850:Chromatography, Gel; D005163:Factor Analysis, Statistical; D019597:Flax / Q000378:metabolism / Q000502:physiology*; D010368:Pectins / Q000378:metabolism; D012639:Seeds / Q000378:metabolism / Q000502:physiology*; D014783:Viscosity; D014990:Xylans / Q000378:metabolism
D014990:Xylans; C042491:rhamnogalacturonan I; D010368:Pectins; C085118:arabinoxylan; C003181:polygalacturonic acid
Arabinoxylan; Factor analyses; Homogalacturonan; Rhamnogalacturonan; Size exclusion chromatography; Vegetative period; Viscosity