Efficient access to the non-reducing end of low molecular weight heparin for fluorescent labeling.
A novel thiol fluorophore was synthesized to be selectively attached to the non-reducing end of low molecular weight heparin (LMWH) via a Michael addition. Double labeling of LMWH was demonstrated to be a feasible approach for the determination of heparinase II activity by FRET.
State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, 24 Tongjia Street, Nanjing, Jiangsu Province 210009, People's Republic of China. yjchen@cpu.edu.cn.
Zongqiang
Z
Wang
Chen
C
Shi
Xuri
X
Wu
Yijun
Y
Chen
D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry*; D010084:Oxidation-Reduction; D011133:Polysaccharide-Lyases / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis
D005456:Fluorescent Dyes; D006025:Glycosaminoglycans; D006495:Heparin, Low-Molecular-Weight; D013438:Sulfhydryl Compounds; D011133:Polysaccharide-Lyases; C114663:heparinase II