p53 Codon 72 Genetic Polymorphism in Asthmatic Children: Evidence of Interaction With Acid Phosphatase Locus 1.
Several lines of evidence are implicating an increased persistence of apoptotic cells in patients with asthma. This is largely due to a combination of inhibition, or defects in the apoptotic process and/or impaired apoptotic cell removal mechanisms. Among apoptosis-inducing genes, an important role is played by p53. In the present study, we have investigated the possible relationship between p53 codon 72 polymorphism and asthma and the interaction with ACP1, a genetic polymorphism involved in the susceptibility to allergic asthma. We studied 125 asthmatic children and 123 healthy subjects from the Caucasian population of Central Italy. p53 codon 72 and ACP1 polymorphisms were evaluated using a restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) method. There is a statistically significant association between p53 codon 72 polymorphism and allergic asthma: Arg/Arg genotype is more represented in asthmatic patients than in controls (P=0.018). This association, however, is present in subjects with low ACP1 activity A/A and A/B only (P=0.023). The proportion of children with A/A and A/B genotype carrying Arg/Arg genotype is significantly high in asthmatic children than in controls (OR=1.941; 95% C.I. 1.042-3.628). Our finding could have important clinical implications since the subjects with A/A and A/B genotypes of ACP1 carrying Arg/Arg genotype are more susceptible to allergic asthma than Pro/Pro genotype.
Department of Biomedicine and Prevention, University of Rome Tor Vergata, School of Medicine, Rome, Italy.
Patrizia
P
Saccucci
Department of Pediatrics, University of Chieti Gabriele D'Annunzio, Chieti, Italy.
Alberto
A
Verrotti
Department of Pediatrics, University of Chieti Gabriele D'Annunzio, Chieti, Italy.
Cosimo
C
Giannini
Department of Pediatrics, University of Chieti Gabriele D'Annunzio, Chieti, Italy.
Marcello
M
Verini
Department of Pediatrics, University of Chieti Gabriele D'Annunzio, Chieti, Italy.
Francesco
F
Chiarelli
Department of Biomedicine and Prevention, University of Rome Tor Vergata, School of Medicine, Rome, Italy.
Anna
A
Neri
Department of Biomedicine and Prevention, University of Rome Tor Vergata, School of Medicine, Rome, Italy.
Andrea
A
Magrini
ACP1; allergy; apoptosis; asthma; genetic polymorphism; p53