An In vitro Study on Chick Somite Ability to Express Cerberus, Chordin, FGF8, Follistatin, and Noggin Transcripts.
BACKGROUND
In vitro simulation of developmental processes is an invaluable tool to shed light on the intrinsic mechanism of developmental biosystems such as central nervous system in mammals. Chick somites have been used to simulate the neural differentiation of human neural progenitor cells. In the present study, we aimed to indicate whether somites have the ability to express required neural differentiation factors at mRNA level.


METHODS
Chick embryos were isolated from the yolk surface of the fertilized eggs and somites were subsequently isolated from embryos under a dissecting microscope. Total RNA of the somites was extracted and RT-PCR carried out with specific primers of cerberus, chordin, FGF8, follistatin and noggin.


RESULTS
Data showed that five aforementioned factors were co-expressed after 7 days in vitro by somites.


CONCLUSIONS
We concluded that neural induction property of somites appeared by production of required neural differentiation factors including cerberus, chordin, FGF8, follistatin and noggin.
Cell and Molecular Biology Division, Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran.
Samaneh Sadat Hosseini
SS
Farahabadi
Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Khadijeh
K
Karbalaie
Department of Anatomy, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Hossein
H
Salehi
Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Farzaneh
F
Rabiee
Cell and Molecular Biology Division, Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran ; Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran ; These authors equally contributed to this work.
Kamran
K
Ghaedi
Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran ; These authors equally contributed to this work.
Mohammad-Hossein
MH
Nasr-Esfahani
Cerberus protein; Chordin; FGF8 protein; Follistatin; Noggin protein; Somites