SEC14 phospholipid transfer protein is involved in lipid signaling-mediated plant immune responses in Nicotiana benthamiana.
We previously identified a gene related to the SEC14-gene phospholipid transfer protein superfamily that is induced in Nicotiana benthamiana (NbSEC14) in response to infection with Ralstonia solanacearum. We here report that NbSEC14 plays a role in plant immune responses via phospholipid-turnover. NbSEC14-silencing compromised expression of defense-related PR-4 and accumulation of jasmonic acid (JA) and its derivative JA-Ile. Transient expression of NbSEC14 induced PR-4 gene expression. Activities of diacylglycerol kinase, phospholipase C and D, and the synthesis of diacylglycerol and phosphatidic acid elicited by avirulent R. solanacearum were reduced in NbSEC14-silenced plants. Accumulation of signaling lipids and activation of diacylglycerol kinase and phospholipases were enhanced by transient expression of NbSEC14. These results suggest that the NbSEC14 protein plays a role at the interface between lipid signaling-metabolism and plant innate immune responses.
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University, Nankoku, Kochi, Japan.
Akinori
A
Kiba
Institute of Plant Science and Resources, Okayama University, Kurashiki, Japan.
Ivan
I
Galis
Institute of Plant Science and Resources, Okayama University, Kurashiki, Japan.
Yuko
Y
Hojo
Research Institute of Molecular Genetics, Kochi University, Nankoku, Kochi, Japan.
Kouhei
K
Ohnishi
Laboratory of Defense in Plant-Pathogen Interactions, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya, Japan.
Hirofumi
H
Yoshioka
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University, Nankoku, Kochi, Japan.
Yasufumi
Y
Hikichi
D003517:Cyclopentanes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate; D007532:Isoleucine / Q000031:analogs & derivatives / Q000378:metabolism; D050356:Lipid Metabolism; D054883:Oxylipins / Q000378:metabolism; D049294:Phospholipid Transfer Proteins / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D043368:Ralstonia solanacearum / Q000502:physiology*; D015398:Signal Transduction; D014026:Nicotiana / Q000378:metabolism*
D003517:Cyclopentanes; D054883:Oxylipins; D049294:Phospholipid Transfer Proteins; D010940:Plant Proteins; C532883:jasmonoyl-isoleucine; D007532:Isoleucine; C011006:jasmonic acid