Zebrafish-based systems pharmacology of cancer metastasis.
Because of their small size, high fecundity, and commonality to human genetics and genomics, phenotype-based animal testing using zebrafish (Danio rerio) has emerged as a powerful tool for identifying disease mechanisms, drug target molecules and small bioactive compounds over the last decade. Importantly, the immaturity of the zebrafish larvae immune system compared with that of mammals facilitates the implantation of human tumors representing aggressive cancer progression with metastasis. In the current chapter, we describe the methods for human cancer cell xenotransplantation into zebrafish, phenotypic image analysis, and transcriptome analysis using deep sequencing.
Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan.
Yasuhito
Y
Shimada
Yuhei
Y
Nishimura
Toshio
T
Tanaka
D000818:Animals; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D019295:Computational Biology; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D057054:Molecular Imaging; D009362:Neoplasm Metastasis; D010190:Pancreatic Neoplasms / Q000473:pathology; D015027:Zebrafish
D005456:Fluorescent Dyes