Antiproliferative protein from the culture supernatant of Lentinula edodes C91-3 mycelia.
We purified and isolated a novel protein (LFP(91-3)A2) with antitumor effect from Lentinula edodes C(91-3) liquid mycelial culture supernatant. LFP(91-3)A2 was purified by (NH4)2SO4 precipitation, ion-exchange chromatography (DEAE-cellulose) and gel filtration chromatography (Sephacryl S-200HR). SDS-PAGE and MALDI-TOF/MS analysis Mascot search showed LFP(91-3)A2 is a new protein with apparent molecular weight of 26 kDa. The effect on tumor cell proliferation was assessed by using MTT assay in vitro, and the LFP(91-3)A2 reduced tumor cell growth obviously in a dose dependent manner (5-15 μg/mL) (p < 0.05), while it exhibited no toxic effect on normal chick embryo fibroblasts. The antiproliferative mechanism of LFP(91-3)A2 was found to be associated with inducing cell apoptosis by flow cytometry analysis and transmission electron microscopy. The LFP(91-3)A2 is a novel protein from Lentinula edodes with tumor-suppressive activity via inducing apoptosis of tumor cells without toxicity on normal cells and may be beneficial to natural products in clinical treatment.
Department of Microbiology, Colleges of Basic Medical Sciences, Dalian Medical University , 9 Western Section, Lvshun South Street, Lvshunkou District, Dalian 116044, Liaoning Province, P.R. China.
Xingyun
X
Li
Mintao
M
Zhong
Ben
B
Liu
Xiaoli
X
Wang
Lei
L
Liu
Wei
W
Zhang
Min
M
Huang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002642:Chick Embryo; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D025282:Mycelium / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D020942:Shiitake Mushrooms / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
D003470:Culture Media; D005656:Fungal Proteins; D006131:Growth Inhibitors