Polymerase chain reaction melting profiles of Candida glabrata clinical isolates in solid organ transplant recipients in comparison to the other group of surgical patients.
The aim of the retrospective study were to estimate the prevalence of Candida glabrata in liver and kidney transplant recipients compared to patients with short bowel syndrome receiving chronic total parenteral nutrition and relevance of the polymerase chain reaction melting profile (PCR MP) method for Candida glabrata strains differentiation. C. glabrata clinical strains isolated from patients were identified by using standard mycological procedures. The analysis of genetic relatedness of the isolated strains was conducted using the PCR MP method. The prevalence of C. glabrata comprised 29% of all episodes of fungal colonization and infection in solid organ transplant recipients, and 54% of those in hospitalized patients receiving long-term total parenteral nutrition. Among 78 isolates obtained from 55 solid organ transplant recipients and 2 organ donors, 44 different C. glabrata PCR MP fingerprints were observed. Forty-seven organ recipients and one organ donor carried unique C. glabrata strains. Among 37 isolates obtained from 31 patients receiving long-term TPN, 8 different PCR MP profiles of C. glabrata strains were observed. Two patients carried unique C. glabrata strains. Most of the C. glabrata colonization and infections in solid-organ transplant recipients were caused by endogenic strains. Most of the C. glabrata colonization and infections in hospitalized patients receiving long-term total parenteral nutrition could result by patient-to-patient transmission. The results showed that the PCR MP technique is a good discriminatory method for genotyping for C. glabrata strains.
Department of Medical Microbiology, Warsaw Medical University, Warsaw, Poland. Electronic address: marlena.gol@gmail.com.
M
M
Golas
Department of Medical Microbiology, Warsaw Medical University, Warsaw, Poland.
I
I
Netsvyetayeva
Department of Medical Microbiology, Warsaw Medical University, Warsaw, Poland.
K
K
Piskorska
Department of Dentistry Microbiology, Warsaw Medical University, Warsaw, Poland.
M
M
Sikora
Department of Prosthetic Dentistry, Warsaw Medical University, Warsaw, Poland.
M
M
Jaworska-Zaremba
Department of Prosthetic Dentistry, Warsaw Medical University, Warsaw, Poland.
E
E
Mierzwinska-Nastalska
Transplantation Institute, Medical University of Warsaw, Warsaw, Poland.
L
L
Paczek
Department of Dentistry Microbiology, Warsaw Medical University, Warsaw, Poland.
E
E
Swoboda-Kopec
D041221:Candida glabrata / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000302:isolation & purification; D006801:Humans; D016377:Organ Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods*; D013514:Surgical Procedures, Operative
D004271:DNA, Fungal