A lipidomic approach to identify cold-induced changes in Arabidopsis membrane lipid composition.
Lipidomic analysis using electrospray ionization triple quadrupole mass spectrometry can be employed to monitor lipid changes that occur during cold and freezing stress of plants. Here we describe the analysis of Arabidopsis thaliana polar glycerolipids with normal and oxidized acyl chains, sampled during cold and freezing treatments. Mass spectral data are processed using the online capabilities of LipidomeDB Data Calculation Environment.
Division of Biology, Kansas Lipidomics Research Center, Kansas State University, Ackert Hall, Manhattan, KS, 66506, USA.
Hieu Sy
HS
Vu
Sunitha
S
Shiva
Aaron Smalter
AS
Hall
Ruth
R
Welti
D000064:Acclimatization; D017360:Arabidopsis / Q000378:metabolism* / Q000502:physiology; D005615:Freezing; D013058:Mass Spectrometry / Q000379:methods*; D008563:Membrane Lipids / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction
D008563:Membrane Lipids