Escherichia coli viability determination using dynamic light scattering: a comparison with standard methods.
To determine the concentration of bacteria in a sample is important in the food industry, medicine and biotechnology. A disadvantage of the plate-counting method is that a microorganism colony could arise from one cell or from many cells. The other standard methodology, known as optical density determination, is based on the turbidity of a suspension and registers all bacteria, dead and alive. In this article, dynamic light scattering is proposed as a fast and reliable method to determine bacterial viability and, consequently, time evolution. Escherichia coli was selected because this microorganism is well known and easy to handle. A correlation between the data from these three techniques was obtained. We were able to calculate the growth rate, usually determined by plate counting or optical density measurement, using dynamic light scattering and to predict bacterial behavior. An analytical relationship between the colony forming units and the light scattered intensity was also deduced.
Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, 76230, Querétaro, Qro., Mexico, loske@fata.unam.mx.
Achim M
AM
Loske
Elba M
EM
Tello
Susana
S
Vargas
Rogelio
R
Rodriguez
D004926:Escherichia coli / Q000502:physiology*; D008027:Light; D050296:Microbial Viability; D010316:Particle Size; D012542:Scattering, Radiation