The complete mitochondrial genome of the ruby-topaz hummingbird Chrysolampis mosquitus through Illumina sequencing.
The complete mitochondrial genome of the Ruby-Topaz Hummingbird, Chrysolampis mosquitus, was determined using 1/11 of an Illumina Hi-seq lane ran with a Nextera kit. We assembled the mitogenome in a two-step approach using both (i) de novo (SOAPdenovo-Trans) and (ii) reference-based (MITObim) genome assembly software. A circular molecule containing 17,767 bp was assembled. As expected for most vertebrates, the C. mosquitus mitogenome contained 13 protein-coding genes, 22 transfer RNA, 2 ribosomal RNA genes, and 1 non-coding control region. We assembled the whole mitogenome using 0.45% of the total amount of reads and produced a high-coverage mitochondrial genome (>1000×). We deposited the assembled mitogenome into GenBank (accession number KJ619585).
a Genomics and Biodiversity Laboratory , Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil .
Helena Magarinos
HM
Souto
b Molecular Laboratory of Biodiversity Research, Departamento de Vertebrados , Museu Nacional, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil .|c Museu de Biologia Prof. Mello Leitão/MBML , Santa Teresa , Espírito Santo , Brazil , and.
Piero Angeli
PA
Ruschi
d Division of Genetics , National Cancer Institute , Rio de Janeiro , Brazil.
Carolina
C
Furtado
b Molecular Laboratory of Biodiversity Research, Departamento de Vertebrados , Museu Nacional, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil .
W Bryan
WB
Jennings
a Genomics and Biodiversity Laboratory , Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil .
Francisco
F
Prosdocimi
D000818:Animals; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D001717:Birds / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D054629:Genome, Mitochondrial; D017422:Sequence Analysis, DNA / Q000379:methods*
D004272:DNA, Mitochondrial
Genome assembly; hummingbird; mitogenome