In vivo imaging in an ABSL-3 regional biocontainment laboratory.
The Regional Biocontainment Laboratory (RBL) at the University of Pittsburgh is a state-of-the-art ABSL-3 facility that supports research on highly pathogenic viruses and bacteria. Recent advances in radiologic imaging provide several noninvasive, in vivo imaging modalities that can be used to longitudinally monitor animals following experimental infection or vaccination. The University of Pittsburgh RBL provides digital radiography, bioluminescence imaging, and PET/CT. Operating these platforms in an ABSL-3 poses unique challenges. This review will discuss the development and refinement of these imaging platforms in high containment, emphasizing specific challenges and how they were overcome.
Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Charles A
CA
Scanga
Brian J
BJ
Lopresti
Jaime
J
Tomko
Lonnie J
LJ
Frye
Teresa M
TM
Coleman
Daniel
D
Fillmore
Jonathan P
JP
Carney
Philana L
PL
Lin
JoAnne L
JL
Flynn
Christina L
CL
Gardner
Chengqun
C
Sun
William B
WB
Klimstra
Kate D
KD
Ryman
Douglas S
DS
Reed
Daniel J
DJ
Fisher
Kelly S
KS
Cole
D000818:Animals; D001424:Bacterial Infections / Q000175:diagnosis* / Q000473:pathology*; D003264:Containment of Biohazards; D004195:Disease Models, Animal; D061848:Optical Imaging / Q000379:methods*; D010414:Pennsylvania; D013679:Technology, Radiologic / Q000379:methods; D014777:Virus Diseases / Q000175:diagnosis* / Q000473:pathology*
ABSL-3; IVIS; PET/CT; X-ray; imaging; in vivo