Cideb facilitates the lipidation of chylomicrons in the small intestine.
Cell death-inducing DFF45-like effector b (Cideb), an endoplasmic reticulum (ER)- and lipid droplet (LD)-associated protein, has been shown to play a critical role in maintaining hepatic lipid homeostasis by promoting the lipidation and maturation of VLDL particles. Here, we observed that Cideb is expressed in the jejunum and ileum sections of the small intestine, and its expression was induced by high-fat diet. Intragastric gavage with lipids resulted in the retention of lipids in the intestine in Cideb-deficient mice. In addition, we observed that mice with Cideb deficiency exhibited reduced intestinal chylomicron-TG secretion and increased lipid accumulation in the enterocytes. The sizes of chylomicrons secreted from the small intestine of Cideb-deficient mice were also smaller than those from wild-type mice. Furthermore, the overexpression of Cideb increased TG secretion and reduced lipid accumulation in the enterocyte-like Caco-2 cells. In addition, we proved that Cideb was localized to the ER and LDs and could interact with ApoB48 in Caco-2 cells. Overall, these data revealed that Cideb plays an important role in controlling intestinal chylomicron lipidation.
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China Department of Clinical Diagnosis, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Li-Jun
LJ
Zhang
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China Department of Clinical Diagnosis, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Chao
C
Wang
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Yuan
Y
Yuan
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Hui
H
Wang
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Jie
J
Wu
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Fang
F
Liu
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Le
L
Li
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Xing
X
Gao
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Yuan-Lin
YL
Zhao
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Pei-Zhen
PZ
Hu
Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 10084, China.
Peng
P
Li
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Jing
J
Ye
D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D002914:Chylomicrons / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007421:Intestine, Small / Q000378:metabolism*; D054878:Lipoylation / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains
D051017:Apoptosis Regulatory Proteins; C112511:CIDEB protein, human; D002914:Chylomicrons; C484761:Cideb protein, mouse
cell death-inducing DNA fragmentation factor-α-like effector b; lipid absorption; lipid droplet