Metabolomic prediction of pregnancy viability in superovulated cattle embryos and recipients with fourier transform infrared spectroscopy.
We analyzed embryo culture medium (CM) and recipient blood plasma using Fourier transform infrared spectroscopy (FTIR) metabolomics to identify spectral models predictive of pregnancy outcome. Embryos collected on Day 6 from superovulated cows in 2 countries were individually cultured in synthetic oviduct fluid medium with BSA for 24 h before embryo transfer. Spent CM, blank controls, and plasma samples (Day 0 and Day 7) were evaluated using FTIR. The spectra obtained were analyzed. The discrimination capability of the classifiers was assessed for accuracy, sensitivity (pregnancy), specificity (nonpregnancy), and area under the ROC curve (AUC). Endpoints considered were Day 60 pregnancy and birth. High AUC was obtained for Day 60 pregnancy in CM within individual laboratories (France AUC = 0.751 ± 0.039, Spain AUC = 0.718 ± 0.024), while cumulative data decreased the AUC (AUC = 0.604 ± 0.029). Predictions for CM at birth were lower than Day 60 pregnancy. Predictions with plasma at birth improved cumulative over individual results (Day 0: France AUC = 0.690 ± 0.044; Spain AUC < 0.55; cumulative AUC = 0.747 ± 0.032). Plasma generally predicted pregnancy and birth better than CM. These first results show that FTIR metabolomics could allow the identification of embryos and recipients with improved pregnancy viability, which may contribute to increasing the efficiency of selection schemes based on ET.
Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, Gijón, 33394 Asturias, Spain.
Marta
0000-0002-9832-3523
M
Muñoz
Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, 310 Cedar Street, LSOG 304B, New Haven, CT 06520, USA ; Department of Computer Engineering, Okan University, Tuzla, 34959 Istanbul, Turkey.
Asli
0000-0002-3828-2387
A
Uyar
Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, Gijón, 33394 Asturias, Spain.
Eva
E
Correia
UNCEIA, Department of Research and Development, 13 rue Jouet, 94704 Maisons Alfort, France.
Claire
C
Ponsart
UNCEIA, Station Expérimentale, 484 Chemin Darefin, 38300 Chateauvillain, France.
Catherine
C
Guyader-Joly
U.T.E. Bos-Lugar de Bos, Guísamo, Bergondo, 15640 A Coruña, Spain ; EMBRIOVET S.L., Rois 18 K, Bergondo, 15165 A Coruña, Spain.
Daniel
D
Martínez-Bello
UNCEIA, Department of Research and Development, 13 rue Jouet, 94704 Maisons Alfort, France.
Brigitte
B
Marquant-Le Guienne
Servicios Científico Técnicos (Oviedo), Universidad de Oviedo, Oviedo, 33006 Asturias, Spain.
Alfonso
A
Fernandez-Gonzalez
Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, Gijón, 33394 Asturias, Spain.
Carmen
C
Díez
Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, Gijón, 33394 Asturias, Spain.
Jose Nestor
JN
Caamaño
Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, Gijón, 33394 Asturias, Spain.
Beatriz
B
Trigal
Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, Sweden.
Patrice
P
Humblot
Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, Gijón, 33394 Asturias, Spain.
Susana
S
Carrocera
Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, Gijón, 33394 Asturias, Spain.
David
D
Martin
Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, 310 Cedar Street, LSOG 304B, New Haven, CT 06520, USA.
Emre
0000-0001-7464-8203
E
Seli
Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, Gijón, 33394 Asturias, Spain.
Enrique
0000-0001-9037-9058
E
Gomez
D000818:Animals; D002417:Cattle; D003470:Culture Media; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D055432:Metabolomics / Q000379:methods*; D011247:Pregnancy; D011256:Pregnancy Outcome; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013480:Superovulation / Q000378:metabolism*
D003470:Culture Media