Experimental aerosolized guinea pig-adapted Zaire ebolavirus (variant: Mayinga) causes lethal pneumonia in guinea pigs.
Eight guinea pigs were aerosolized with guinea pig-adapted Zaire ebolavirus (variant: Mayinga) and developed lethal interstitial pneumonia that was distinct from lesions described in guinea pigs challenged subcutaneously, nonhuman primates challenged by the aerosol route, and natural infection in humans. Guinea pigs succumbed with significant pathologic changes primarily restricted to the lungs. Intracytoplasmic inclusion bodies were observed in many alveolar macrophages. Perivasculitis was noted within the lungs. These changes are unlike those of documented subcutaneously challenged guinea pigs and aerosolized filoviral infections in nonhuman primates and human cases. Similar to findings in subcutaneously challenged guinea pigs, there were only mild lesions in the liver and spleen. To our knowledge, this is the first report of aerosol challenge of guinea pigs with guinea pig-adapted Zaire ebolavirus (variant: Mayinga). Before choosing this model for use in aerosolized ebolavirus studies, scientists and pathologists should be aware that aerosolized guinea pig-adapted Zaire ebolavirus (variant: Mayinga) causes lethal pneumonia in guinea pigs.
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD nancy.twenhafel@us.army.mil.
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Twenhafel
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD.
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CI
Shaia
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD.
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Bunton
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD.
J D
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Shamblin
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD.
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Wollen
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD.
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LM
Pitt
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD.
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Sizemore
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD.
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Ogg
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD.
S C
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Johnston
D000336:Aerosols / Q000008:administration & dosage; D000818:Animals; D004195:Disease Models, Animal; D029043:Ebolavirus / Q000502:physiology*; D005260:Female; D006168:Guinea Pigs; D019142:Hemorrhagic Fever, Ebola / Q000473:pathology* / Q000821:virology; D006801:Humans; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology / Q000821:virology; D016676:Macrophages, Alveolar / Q000473:pathology / Q000821:virology; D008297:Male; D011014:Pneumonia / Q000473:pathology* / Q000821:virology; D013154:Spleen / Q000473:pathology / Q000821:virology
D000336:Aerosols
Mayinga; aerosol; alveolar macrophages; animal model; ebolavirus; filovirus; guinea pig; lungs