Identification and mRNA expression of two 17β-hydroxysteroid dehydrogenase genes in the marine mussel Mytilus galloprovincialis following exposure to endocrine disrupting chemicals.
17β-Hydroxysteroid dehydrogenases (17β-HSDs) are multifunctional enzymes involved in the metabolism of steroids, fatty acids, retinoids and bile acid. In this study, two novel types of 17β-HSDs (named as MgHsd17b10 and MgHsd17b12) were cloned from Mytilus galloprovincialis by using rapid amplification of cDNA ends (RACE) approaches. Sequence analysis showed that MgHsd17b10 and MgHsd17b12 encoded a polypeptide of 259 and 325 amino acids, respectively. Phylogenetic analysis revealed that MgHsd17b10 and MgHsd17b12 were evolutionarily clustered with other invertebrate 17β-HSD type 10 and 17β-HSD type 12 homologues. The MgHsd17b10 and MgHsd17b12 transcripts could be detected in all examined tissues with higher expression levels in digestive glands and gonad. After exposed to endocrine disrupting chemicals (Bisphenol A or 2,2',4,4'-tetrabromodiphenyl ether), the expression of MgHsd17b10 and MgHsd17b12 transcripts was both down-regulated in digestive glands. These findings suggest that MgHsd17b10 and MgHsd17b12 perhaps play an important role in the endocrine regulation of M. galloprovincialis.
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Yingying
Y
Zhang
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
Qing
Q
Wang
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Yinglu
Y
Ji
China Agriculture University (Yantai), Yantai 264670, PR China.
Qian
Q
Zhang
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China. Electronic address: hfwu@yic.ac.cn.
Huifeng
H
Wu
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Jia
J
Xie
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China. Electronic address: jmzhao@yic.ac.cn.
Jianmin
J
Zhao
D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001559:Benzhydryl Compounds / Q000633:toxicity*; D018076:DNA, Complementary / Q000235:genetics; D052244:Endocrine Disruptors / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D008969:Molecular Sequence Data; D049878:Mytilus / Q000235:genetics*; D010636:Phenols / Q000633:toxicity*; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000098826:Bisphenol A Compounds
D015067:17-Hydroxysteroid Dehydrogenases; D001559:Benzhydryl Compounds; D018076:DNA, Complementary; D052244:Endocrine Disruptors; D055768:Halogenated Diphenyl Ethers; D010636:Phenols; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; C511295:2,2',4,4'-tetrabromodiphenyl ether; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; C006780:bisphenol A; D000098826:Bisphenol A Compounds
17β-Hydroxysteroid dehydrogenases; Digestive glands; Endocrine disrupting chemicals; Gene expression; Mytilus galloprovincialis