The effect of glycosylation of antigens on the antibody responses against Echinostoma caproni (Trematoda: Echinostomatidae).
In the present study, we analyse the effect of glycosylation in Echinostoma caproni (Trematoda: Echinostomatidae) antigens in antibody responses against the parasite in experimentally infected mice. It has been previously demonstrated that the mouse is a host of high compatibility with E. caproni and develops elevated responses of IgG, IgG1, IgG3 and IgM as a consequence of the infection, though the role of glycans in these responses remains unknown. To this purpose, the responses generated in mice against non-treated excretory/secretory antigens of E. caproni were compared with those observed after N-deglycosylation, O-deglycosylation and double deglycosylation of the antigens by indirect ELISA and western blot. Our results suggest that E. caproni-expressed glycans play a major role in the modulation of the immune responses. The results obtained indicate that IgG subclass responses generated in mice against E. caproni are essentially due to glycoproteins and may affect the Th1/Th2 biasing. The reactivity significantly decreased after any of the deglycosylation treatments and the N-glycans appears to be of greater importance than O-glycans. Interestingly, the IgM response increased after N-deglycosylation suggesting that carbohydrates may mask peptide antigens.
Departamento de Parasitología, Facultad de Farmacia,Universidad de Valencia,Av. Vicente Andrés Estellés s/n, 46100 Burjassot - Valencia,Spain.
Javier
J
Sotillo
Departamento de Parasitología, Facultad de Farmacia,Universidad de Valencia,Av. Vicente Andrés Estellés s/n, 46100 Burjassot - Valencia,Spain.
Alba
A
Cortés
Departamento de Parasitología, Facultad de Farmacia,Universidad de Valencia,Av. Vicente Andrés Estellés s/n, 46100 Burjassot - Valencia,Spain.
Carla
C
Muñoz-Antoli
Department of Biology,Lafayette College,Easton, Pennsylvania 18042,USA.
Bernard
B
Fried
Departamento de Parasitología, Facultad de Farmacia,Universidad de Valencia,Av. Vicente Andrés Estellés s/n, 46100 Burjassot - Valencia,Spain.
J Guillermo
JG
Esteban
Departamento de Parasitología, Facultad de Farmacia,Universidad de Valencia,Av. Vicente Andrés Estellés s/n, 46100 Burjassot - Valencia,Spain.
Rafael
R
Toledo
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D004449:Echinostoma / Q000276:immunology*; D004451:Echinostomiasis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins; D006031:Glycosylation; D006790:Host-Parasite Interactions; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011134:Polysaccharides / Q000276:immunology*
D000947:Antigens, Helminth; D006023:Glycoproteins; D007074:Immunoglobulin G; D011134:Polysaccharides