Metagonimus yokogawai: a 100-kDa somatic antigen commonly reacting with other trematodes.
This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity was assessed by western blot analysis with patients' sera. The mAbs against the 100 kDa antigen commonly reacted with various kinds of trematode antigens, including intestinal (Gymnophalloides seoi), lung (Paragonimus westermani), and liver flukes (Clonorchis sinensis and Fasciola hepatica). However, this mAb showed no cross-reactions with other helminth parasites, including nematodes and cestodes. To determine the topographic distribution of the 100 kDa antigen in worm sections, indirect immunoperoxidase staining was performed. A strong positive reaction was observed in the tegumental and subtegumental layers of adult M. yokogawai and C. sinensis. The results showed that the 100 kDa somatic protein of M. yokogawai is a common antigen which recognizes a target epitope present over the tegumental layer of different trematode species.
Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, Korea.
Eun-Taek
ET
Han
Department of Parasitology, College of Medicine, Ewha Womans University, Seoul 158-056, Korea.
Hyun-Jong
HJ
Yang
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul National University, Seoul 110-799, Korea.
Young-Jin
YJ
Park
Department of Anatomy, School of Medicine, Kangwon National University, Chuncheon 200-701, Korea.
Jeong-Hyun
JH
Park
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul National University, Seoul 110-799, Korea.
Jong-Yil
JY
Chai
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D003004:Clonorchis sinensis / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D005210:Fasciola hepatica / Q000276:immunology; D005260:Female; D015801:Helminth Proteins / Q000276:immunology*; D006578:Heterophyidae / Q000276:immunology*; D007159:Immunologic Tests; D051379:Mice; D008807:Mice, Inbred BALB C; D048492:Paragonimus westermani / Q000276:immunology; D014201:Trematode Infections / Q000175:diagnosis* / Q000276:immunology
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D015801:Helminth Proteins
100 kDa protein; Metagonimus yokogawai; common trematode antigen; immunoblot; immunohistochemistry; tegument