Membrane curvature modulation of protein activity determined by NMR.
In addition to specific intermolecular interactions, biological processes at membranes are also modulated by the physical properties of the membrane. One of these properties is membrane curvature. NMR methods are useful for studying how membrane curvature affects the binding and insertion of proteins into membranes as well as how proteins can affect membrane curvature properties. In many cases these interactions result in a marked change in protein activity. We have reviewed examples from a range of systems having varied mechanisms by which membrane curvature is linked to protein activity. Among the examples discussed are antimicrobial peptides, proteins affecting membrane fusion, rhodopsin, protein kinase C, phospholipase C-delta1, phosphatidylinositol-3 kinase-related kinases and tafazzin.
Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada. Electronic address: epand@mcmaster.ca.
Richard M
RM
Epand
Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada.
Kenneth
K
D'Souza
Department of Chemistry and Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada.
Bob
B
Berno
Department of Anesthesiology and Cell Biology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.
Michael
M
Schlame
D000217:Acyltransferases; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000737:chemistry*; D012243:Rhodopsin / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry
D023181:Antimicrobial Cationic Peptides; D008565:Membrane Proteins; D014157:Transcription Factors; D012243:Rhodopsin; D000217:Acyltransferases; C108675:TAFAZZIN protein, human
CSA; Lipid polymorphism; MAS; Membrane curvature; Membrane-bound enzyme; Tafazzin