Changes of serum adhesion molecules and cytokines in post-ERCP pancreatitis: adhesion molecules and cytokines in acute pancreatitis.
OBJECTIVE
To assess the early changes of soluble IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, TNF-α, TNF-β, IL-17A, IL-22, soluble (s) P-Selectin, sE-Selectin and sICAM-1 in post-ERCP pancreatitis (PEP).


METHODS
Single center, prospective study of 318 ERCP procedures. Serum samples were acquired from all patients prior to ERCP, 6 hours and 24 hours after the procedure. For every PEP case, another patient was chosen as a control, matched for gender, age and time period in which ERCP took place.


RESULTS
Totally, 28 cases and 28 controls were studied. Except for significantly higher IL-1b levels in cases at baseline, no significant differences were observed between cases and controls after Bonferroni corrections. An increase in IL-6 was noted between baseline and 6 h in cases alone (p=0.016). There was a significant fall in sP-selectin levels at 6 and 24 hours compared to baseline in all patients (corrected p=0.008 and 0.016 for cases and 0.016 and 0.048 for controls respectively). An increase of sE-selectin in cases was observed between 6 and 24 hours post-ERCP (corrected p=0.03).


CONCLUSIONS
Soluble forms of cytokines and adhesion molecules studied seem not to play a major role in PEP.
1(st) Division of Internal Medicine and Division of Gastroenterology, Medical School, University of Ioannina, Ioannina, Greece.
Dimitrios E
DE
Sigounas
1(st) Division of Internal Medicine and Division of Gastroenterology, Medical School, University of Ioannina, Ioannina, Greece.
Dimitrios K
DK
Christodoulou
Hematology Laboratory, University Hospital of Ioannina, Ioannina, Greece.
Achilleas
A
Karamoutsios
Department of Internal Medicine, University of Ioannina School of Medicine, Ioannina, Greece; Department of Medicine, Tufts University School of Medicine and Institute for Clinical Research and Health Policy Studies, Tufts Medical Center, Boston, MA, USA.
Athina
A
Tatsioni
Hematology Laboratory, University Hospital of Ioannina, Ioannina, Greece.
Lefkothea
L
Dova
Hematology Laboratory, University Hospital of Ioannina, Ioannina, Greece.
Georgios
G
Vartholomatos
Hematology Laboratory, University Hospital of Ioannina, Ioannina, Greece.
Nikolaos
N
Kolaitis
1(st) Division of Internal Medicine and Division of Gastroenterology, Medical School, University of Ioannina, Ioannina, Greece.
Konstantinos H
KH
Katsanos
Blood bank, University Hospital of Ioannina, Ioannina, Greece.
Eleftheria
E
Zervou
Stanford Prevention Research Center, Department of Medicine and Department of Health Research and Policy, Stanford University School of Medicine, Stanford, CA, USA.
John P A
JP
Ioannidis
1(st) Division of Internal Medicine and Division of Gastroenterology, Medical School, University of Ioannina, Ioannina, Greece. Electronic address: etsianos@uoi.gr.
Epameinondas V
EV
Tsianos
D000368:Aged; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000097:blood*; D002760:Cholangiopancreatography, Endoscopic Retrograde / Q000379:methods; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010195:Pancreatitis / Q000097:blood*; D011446:Prospective Studies
D015815:Cell Adhesion Molecules; D016207:Cytokines
Adhesion molecules; Cytokines; ELISA; ERCP; Flow cytometry; Pancreatitis