Regulation of jasmonic acid biosynthesis by silicon application during physical injury to Oryza sativa L.
We investigated the effects of silicon (Si) application on rice plants (Oryza sativa L.) and its responses in the regulation of jasmonic acid (JA) during wounding stress. Endogenous JA was significantly higher in wounded rice plants than in non-wounded. In contrast, Si treatment significantly reduced JA synthesis as compared to non-Si applications under wounding stress. mRNA expression of O. sativa genes showed down-regulation of lipoxygenase, allene oxide synthase 1, allene oxide synthase 2, 12-oxophytodienoate reductase 3, and allene oxide cyclase upon Si application and wounding stress as compared to non-Si-treated wounded rice plants. The physical injury-induced-oxidative stress was modulated by Si treatments, which resulted in higher catalase, peroxidase, and polyphenol oxidase activities as compared with non-Si-treated plants under wounding stress. The higher Si accumulation in rice plants also reduced the level of lipid peroxidation, which helped the rice plants to protect it from wounding stress. In conclusion, Si accumulation in rice plants mitigated the adverse effects of wounding through regulation of antioxidants and JA.
School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of Korea.
Yoon-Ha
YH
Kim
Abdul Latif
AL
Khan
Muhammad
M
Waqas
Hee-Jeong
HJ
Jeong
Duk-Hwan
DH
Kim
Jeong Sheop
JS
Shin
Jong-Guk
JG
Kim
Myung-Hun
MH
Yeon
In-Jung
IJ
Lee
D003517:Cyclopentanes / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D012275:Oryza / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D018384:Oxidative Stress / Q000187:drug effects; D054883:Oxylipins / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012825:Silicon / Q000378:metabolism* / Q000494:pharmacology*
D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; D012825:Silicon