Production of structured triacylglycerols from microalgae.
Structured triacylglycerols (TAGs) were isolated from nine cultivated strains of microalgae belonging to different taxonomic groups, i.e. Audouinella eugena, Balbiania investiens, Myrmecia bisecta, Nannochloropsis limnetica, Palmodictyon varium, Phaeodactylum tricornutum, Pseudochantransia sp., Thorea ramosissima, and Trachydiscus minutus. They were separated and isolated by means of NARP-LC/MS-APCI and chiral LC and the positional isomers and enantiomers of TAGs with two polyunsaturated, i.e. arachidonic (A) and eicosapentaenoic (E) acids and one saturated, i.e. palmitic acid (P) were identified. Algae that produce eicosapentaenoic acid were found to biosynthesize more asymmetrical TAGs, i.e. PPE or PEE, whereas algae which produced arachidonic acid give rise to symmetrical TAGs, i.e. PAP or APA, irrespective of their taxonomical classification. Nitrogen and phosphorus starvation consistently reversed the ratio of asymmetrical and symmetrical TAGs.
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic. Electronic address: rezanka@biomed.cas.cz.
Tomáš
T
Řezanka
Institute of Botany, Academy of Sciences of the Czech Republic, Centre for Bioindication and Revitalization, Dukelská 135, 379 82 Třeboň, Czech Republic.
Jaromír
J
Lukavský
Institute of Botany, Academy of Sciences of the Czech Republic, Centre for Bioindication and Revitalization, Dukelská 135, 379 82 Třeboň, Czech Republic; Charles University in Prague, Faculty of Science, Department of Ecology, Viničná 7, 128 44 Prague 2, Czech Republic.
Linda
L
Nedbalová
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic.
Karel
K
Sigler
D016718:Arachidonic Acid / Q000378:metabolism; D000460:Chlorophyta / Q000737:chemistry*; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D058086:Microalgae / Q000737:chemistry*; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D000461:Rhodophyta / Q000737:chemistry*; D058009:Stramenopiles / Q000737:chemistry*; D014280:Triglycerides / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*
D005227:Fatty Acids; D014280:Triglycerides; D016718:Arachidonic Acid; D010758:Phosphorus; D015118:Eicosapentaenoic Acid; D009584:Nitrogen
Chiral LC; Enantiomers; Microalgae; NARP-LC/MS-APCI; Polyunsaturated fatty acids; Polyunsaturated triacylglycerols; Structured triacylglycerols