Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level.
Developing methods that evaluate the cellular uptake of magnetic nanoparticles (MNPs) and nanotoxicity effects at single-cellular level are needed. In this study, magnetophoresis combining fluorescence based cytotoxicity assay was proposed to assess the viability and the single-cellular MNPs uptake simultaneously. Malignant cells (SKHep-1, HepG2, HeLa) were incubated with 10 nm anionic iron oxide nanoparticles. Prussian blue stain was performed to visualize the distribution of magnetic nanoparticles. MTT and fluorescence based assay analyzed the cytotoxicity effects of the bulk cell population and single cell, respectively. DAPI/PI stained was applied to evaluate death mechanism. The number of intracellular MNPs was found to be strongly correlated with the cell death. Significant differences between cellular MNP uptake in living and dead cells were observed. The method could be useful for future study of the nanotoxicity induced by MNPs.
Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Chen-Yu
CY
Huang
Institute of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu, Taiwan.
Tzong-Rong
TR
Ger
Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Zung-Hang
ZH
Wei
Institute of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu, Taiwan.
Mei-Feng
MF
Lai
D017209:Apoptosis; D005290:Ferric Compounds; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D058185:Magnetite Nanoparticles; D053758:Nanoparticles; D009336:Necrosis; D010316:Particle Size
D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide