Fluid shear stress threshold regulates angiogenic sprouting.
The density and architecture of capillary beds that form within a tissue depend on many factors, including local metabolic demand and blood flow. Here, using microfluidic control of local fluid mechanics, we show the existence of a previously unappreciated flow-induced shear stress threshold that triggers angiogenic sprouting. Both intraluminal shear stress over the endothelium and transmural flow through the endothelium above 10 dyn/cm(2) triggered endothelial cells to sprout and invade into the underlying matrix, and this threshold is not impacted by the maturation of cell-cell junctions or pressure gradient across the monolayer. Antagonizing VE-cadherin widened cell-cell junctions and reduced the applied shear stress for a given transmural flow rate, but did not affect the shear threshold for sprouting. Furthermore, both transmural and luminal flow induced expression of matrix metalloproteinase 1, and this up-regulation was required for the flow-induced sprouting. Once sprouting was initiated, continuous flow was needed to both sustain sprouting and prevent retraction. To explore the potential ramifications of a shear threshold on the spatial patterning of new sprouts, we used finite-element modeling to predict fluid shear in a variety of geometric settings and then experimentally demonstrated that transmural flow guided preferential sprouting toward paths of draining interstitial fluid flow as might occur to connect capillary beds to venules or lymphatics. In addition, we show that luminal shear increases in local narrowings of vessels to trigger sprouting, perhaps ultimately to normalize shear stress across the vasculature. Together, these studies highlight the role of shear stress in controlling angiogenic sprouting and offer a potential homeostatic mechanism for regulating vascular density.
Departments of Bioengineering,Physiology, and.
Peter A
PA
Galie
Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA 19104;
Duc-Huy T
DH
Nguyen
Departments of Bioengineering.
Colin K
CK
Choi
Departments of Bioengineering.
Daniel M
DM
Cohen
Physiology, and.
Paul A
PA
Janmey
Departments of Bioengineering,Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA 19104;Department of Biomedical Engineering, Boston University, Boston, MA 02215; andThe Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115 chencs@bu.edu.
Christopher S
CS
Chen
D000256:Adenoviridae / Q000235:genetics; D002196:Capillaries / Q000166:cytology / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D020342:Finite Element Analysis; D020868:Gene Silencing; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000502:physiology*; D040542:Mechanotransduction, Cellular / Q000502:physiology; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D018919:Neovascularization, Physiologic / Q000502:physiology*; D013314:Stress, Mechanical
C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
angiogenesis; force; mechanotransduction; migration; morphogenesis