Protective effect of Salvia miltiorrhiza aqueous extract on myocardium oxidative injury in ischemic-reperfusion rats.
Salvia miltiorrhiza has strong antioxidative activity. They may have a strong potential as cardioprotective agents in ischemic-reperfusion injury. Experiments were carried out in Sprague-Dawley rats with myocardium ischemia reperfusion (IR). Myocardial injuries during IR were determined by changes in electrocardiogram analysis of arrhythmias, antioxidant enzyme activities, AST, CK-MB, lactate dehydrogenase (LDH) levels, and myocyte apoptosis. Results showed that S. miltiorrhiza aqueous extract (SAME) pre-treatment significantly decreased the ST-segment (ΣST120) and myocardium MDA, AST, CK-MB, lactate dehydrogenase (LDH) levels, increased myocardium antioxidant enzyme activities, and inhibit myocardium cell apoptosis. Furthermore, the SAME pre-treatment significantly upregulated p-JAK2 and p-STAT3 protein expression, decreased myocardium TNF-α and IL-6 concentrations in IR rats. The levels of TNF-α and IL-6 were positively correlated with the changes in myocardium p-JAK2 and p-STAT3 protein expression levels in IR rats. It can be concluded that the SAME pre-treatment has anti-ischemic and anti-apoptosis activity in heart IR rats. SAME pre-treatment protects heart against IR injury, at least in part, through its stimulating effects on injury-induced deactivation of JAK2/STAT3 signaling pathway.
Department of Cardiology, Fengxian Branch of Shanghai 6th People's Hospital, Shanghai 201400, China.
Guanghao
G
Ge
Department of Cardiology, Fengxian Branch of Shanghai 6th People's Hospital, Shanghai 201400, China.
Qiong
Q
Zhang
Department of Cardiology, Fengxian Branch of Shanghai 6th People's Hospital, Shanghai 201400, China. Electronic address: jiangwma013@163.com.
Jiangwei
J
Ma
Department of Cardiology, Fengxian Branch of Shanghai 6th People's Hospital, Shanghai 201400, China. Electronic address: zengyongqsp@163.com.
Zengyong
Z
Qiao
Department of Cardiology, Fengxian Branch of Shanghai 6th People's Hospital, Shanghai 201400, China.
Jianhua
J
Huang
Department of Cardiology, Fengxian Branch of Shanghai 6th People's Hospital, Shanghai 201400, China.
Wenbo
W
Cheng
Department of Cardiology, Fengxian Branch of Shanghai 6th People's Hospital, Shanghai 201400, China.
Hongwei
H
Wang
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004798:Enzymes / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D053614:Janus Kinase 2 / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D050796:STAT3 Transcription Factor / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000975:Antioxidants; D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D004798:Enzymes; D015850:Interleukin-6; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; C507927:Jak2 protein, rat; D053614:Janus Kinase 2
Antioxidant; JAK2/STAT3 signaling pathway; Myocardium ischemia reperfusion; Salvia miltiorrhiza aqueous extract