Unusual multiple production of N-acylhomoserine lactones a by Burkholderia sp. strain C10B isolated from dentine caries.
Bacteria realize the ability to communicate by production of quorum sensing (QS) molecules called autoinducers, which regulate the physiological activities in their ecological niches. The oral cavity could be a potential area for the presence of QS bacteria. In this study, we report the isolation of a QS bacterial isolate C10B from dentine caries. Preliminary screening using Chromobacterium violaceum CV026 biosensor showed that isolate C10B was able to produce N-acylhomoserine lactones (AHLs). This bacterium was further identified as a member of Burkholderia, an opportunistic pathogen. The isolated Burkholderia sp. was confirmed to produce N-hexanoyl-L-homoserine lactone (C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-decanoyl-L-homoserine lactone (C10-HSL) and N-dodecanoyl-L-homoserine lactone (C12-HSL).
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. shareyuan@um.edu.my.
Share Yuan
SY
Goh
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. tmarilyn36@gmail.com.
Wen-Si
WS
Tan
Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia. saadkhan@um.edu.my.
Saad Ahmed
SA
Khan
Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia. chewhp@um.edu.my.
Hooi Pin
HP
Chew
Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia. nhayaty@um.edu.my.
Noor Hayaty
NH
Abu Kasim
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. yinwaifong@yahoo.com.
Wai-Fong
WF
Yin
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. kokgan@um.edu.my.
Kok-Gan
KG
Chan
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D054742:Acyl-Butyrolactones / Q000032:analysis / Q000378:metabolism*; D015374:Biosensing Techniques; D019117:Burkholderia / Q000302:isolation & purification* / Q000378:metabolism* / Q000472:pathogenicity; D002853:Chromatography, Liquid; D002861:Chromobacterium; D003731:Dental Caries / Q000382:microbiology*; D006714:Homoserine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D007783:Lactones / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D054742:Acyl-Butyrolactones; D007783:Lactones; C104908:N-hexanoyl-L-homoserine lactone; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D015107:4-Butyrolactone