Exploring metabolic syndrome serum profiling based on gas chromatography mass spectrometry and random forest models.
Metabolic syndrome (MetS) is a constellation of the most dangerous heart attack risk factors: diabetes and raised fasting plasma glucose, abdominal obesity, high cholesterol and high blood pressure. Analysis and representation of the variances of metabolic profiles is urgently needed for early diagnosis and treatment of MetS. In current study, we proposed a metabolomics approach for analyzing MetS based on GC-MS profiling and random forest models. The serum samples from healthy controls and MetS patients were characterized by GC-MS. Then, random forest (RF) models were used to visually discriminate the serum changes in MetS based on these GC-MS profiles. Simultaneously, some informative metabolites or potential biomarkers were successfully discovered by means of variable importance ranking in random forest models. The metabolites such as 2-hydroxybutyric acid, inositol and d-glucose, were defined as potential biomarkers to diagnose the MetS. These results obtained by proposed method showed that the combining GC-MS profiling with random forest models was a useful approach to analyze metabolites variances and further screen the potential biomarkers for MetS diagnosis.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Zhang
Z
Lin
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China; Universidad de Cádiz, Faculdad de Ciencias, Departamento de Química Analítica, España.
Carlos M
CM
Vicente Gonçalves
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Ling
L
Dai
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: hongmeilu@csu.edu.cn.
Hong-mei
HM
Lu
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: huangjh85@gmail.com.
Jian-hua
JH
Huang
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Hongchao
H
Ji
Xiangya Hospital, Central South University, Changsha 410008, PR China.
Dong-sheng
DS
Wang
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Lun-zhao
LZ
Yi
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Yi-zeng
YZ
Liang
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood* / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D008962:Models, Theoretical
D015415:Biomarkers
Biomarker; GC–MS; Metabolic syndrome; Random forest; Serum profiling