First successful pregnancy in a globozoospermic patient having homozygous mutation in SPATA16.
OBJECTIVE
To report the first case of a successful pregnancy obtained with an infertile 29-year-old man with a homozygous mutation in the spermatogenesis-specific gene SPATA16 after intracytoplasmic sperm injection (ICSI).


METHODS
Case report.


METHODS
Assisted reproductive technology (ART) center.


METHODS
A couple who suffered from infertility of whom the husband was globozoospermic and carrying a homozygous mutation in the spermatogenesis-specific gene SPATA16.


METHODS
Clinical and histopathological evaluation, transmission electron microscopy, stimulation with fixed antagonist protocol, ICSI, activation of fertilization.


METHODS
In vitro fertilization and pregnancy.


RESULTS
Oligoasthenoteratozoospermia was revealed in semen analysis. Total globozoospermia was detected. Chromatin condensation pattern was detected abnormal in 88% of spermatozoa with aniline blue staining. During the ART cycle 12 oocytes were collected. Eleven oocytes were at the metaphase II and one was at the germinal vesicle stage. After the injection, one oocyte fertilized on the following day; the 4-cell stage embryo was transferred. Pregnancy occurred and a healthy boy was delivered after 42 weeks of gestation.


CONCLUSIONS
This is the first report of a pregnancy obtained in a man with homozygous SPATA16 mutated globozoospermia.
IVF Center, 29 Mayis Hospital, Istanbul, Turkey. Electronic address: karacanilay@hotmail.com.
Nilay
N
Karaca
IVF Center, 29 Mayis Hospital, Istanbul, Turkey.
Rabiye
R
Yilmaz
IVF Center, 29 Mayis Hospital, Istanbul, Turkey.
Gulcin E
GE
Kanten
Histology and Embryology Department, Istanbul University, Istanbul School of Medicine, Istanbul, Turkey.
Elif
E
Kervancioglu
Histology and Embryology Department, Marmara University, School of Medicine, Istanbul, Turkey.
Seyhun
S
Solakoglu
Obstetric and Gynecology Department, IVF Center, Istanbul University, Cerrahpasa School of Medicine, Istanbul University, Istanbul, Turkey.
M Ertan
ME
Kervancioglu
D000328:Adult; D004252:DNA Mutational Analysis; D004624:Embryo Transfer; D005260:Female; D020022:Genetic Predisposition to Disease; D018398:Homeodomain Proteins / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000235:genetics* / Q000503:physiopathology / Q000628:therapy; D050498:Live Birth; D008297:Male; D009154:Mutation; D010641:Phenotype; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D013091:Spermatogenesis / Q000235:genetics*; D016896:Treatment Outcome; D033921:Vesicular Transport Proteins
D018398:Homeodomain Proteins; C474264:SPATA16 protein, human; D033921:Vesicular Transport Proteins
Globozoospermia; SPATA16 gene mutation; intracytoplasmic sperm injection