Visual detection of melamine based on the peroxidase-like activity enhancement of bare gold nanoparticles.
In this study, a facile method to sensitively detect melamine and highly improve the peroxidase-like activity of bare gold nanoparticles (Au NPs) at the same time is proposed for the first time. It is interesting to find that the addition of melamine could improve the peroxidase-like activity of Au NPs. By coupling with 3,3',5,5'-tetramethlybenzidine (TMB)-H2O2 chormogenic reaction, a novel method for colorimetic detection of melamine is developed. The detection limit of this method is as low as 0.2 nM with the help of UV-vis spectroscopy and 0.5 µM by naked-eye observation, both which are far below the US food and Drug Administration estimated melamine safety limit of 20 µM. In addition, the present method is successfully applied for the detection of melamine in raw milk and milk powder. More importantly, the proposed method could also improve the peroxidase-like activity of Au NPs, which may not only provide a new approach to develop effective nanomaterials-based mimetic enzyme, but also irradiative to develop new applications for Au NPs in varieties of cost-effective and simple sensors in medicine, biotechnology and environmental chemistry.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People׳s Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People׳s Republic of China.
Pengjuan
P
Ni
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People׳s Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People׳s Republic of China.
Haichao
H
Dai
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People׳s Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People׳s Republic of China.
Yilin
Y
Wang
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People׳s Republic of China.
Yujing
Y
Sun
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People׳s Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People׳s Republic of China.
Yan
Y
Shi
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People׳s Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People׳s Republic of China.
Jingting
J
Hu
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People׳s Republic of China. Electronic address: zli@ciac.ac.cn.
Zhuang
Z
Li
D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014227:Triazines / Q000032:analysis*
D014227:Triazines; D006046:Gold; D009195:Peroxidase; C011907:melamine
Gold nanoparticles; Melamine; Peroxidase-like activity; Visual detection