Mutations in SACPD-C result in a range of elevated stearic acid concentration in soybean seed.
Soybean oil has a wide variety of uses, and stearic acid, which is a relatively minor component of soybean oil is increasingly desired for both industrial and food applications. New soybean mutants containing high levels of the saturated fatty acid stearate in seeds were recently identified from a chemically mutagenized population. Six mutants ranged in stearate content from 6-14% stearic acid, which is 1.5 to 3 times the levels contained in wild-type seed of the Williams 82 cultivar. Candidate gene sequencing revealed that all of these lines carried amino acid substitutions in the gene encoding the delta-9-stearoyl-acyl-carrier protein desaturase enzyme (SACPD-C) required for the conversion of stearic acid to oleic acid. Five of these missense mutations were in highly conserved residues clustered around the predicted di-iron center of the SACPD-C enzyme. Co-segregation analysis demonstrated a positive association of the elevated stearate trait with the SACPD-C mutation for three populations. These missense mutations may provide additional alleles that may be used in the development of new soybean cultivars with increased levels of stearic acid.
Crop Production and Pest Control Research Unit, Agricultural Research Service (ARS), United States Department of Agriculture (USDA), West Lafayette, Indiana, United States of America.
Militza
M
Carrero-Colón
Department of Agronomy, Purdue University, West Lafayette, Indiana, United States of America.
Nathan
N
Abshire
Department of Agronomy, Purdue University, West Lafayette, Indiana, United States of America.
Daniel
D
Sweeney
Department of Agronomy, Purdue University, West Lafayette, Indiana, United States of America.
Erik
E
Gaskin
Crop Production and Pest Control Research Unit, Agricultural Research Service (ARS), United States Department of Agriculture (USDA), West Lafayette, Indiana, United States of America.
Karen
K
Hudson
D000595:Amino Acid Sequence; D005227:Fatty Acids / Q000378:metabolism; D005838:Genotype; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012639:Seeds / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism*
D005227:Fatty Acids; D013229:Stearic Acids; C031183:stearic acid; D006899:Mixed Function Oxygenases; C018747:acyl-(acyl-carrier-protein)desaturase