Demonstration of Coccidioides immitis and Coccidioides posadasii DNA in soil samples collected from Dinosaur National Monument, Utah.
Soil samples were collected in 2006 from Dinosaur National Monument (DNM), Utah, the site of an outbreak of coccidioidomycosis in 2001. DNA was isolated from two soil samples, and polymerase chain reaction (PCR) amplified Coccidioides DNA present in both samples. Ribosomal RNA genes and internal transcribed spacer (ITS) region PCR products were sequenced. Single-nucleotide polymorphisms indicated that the DNA from sample SS06RH was that of Coccidioides immitis, while the DNA from sample SS06UM was C. posadasii. This is the first report to directly demonstrate Coccidioides in soils from DNM and the first to report the presence of both C. immitis and C. posadasii in the same geographic location.
Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, California smjohnson@ucdavis.edu.
Suzanne M
SM
Johnson
Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, California.
Erin L
EL
Carlson
Department of Geosciences, University of Arizona, Tucson, Arizona.
Frederick S
FS
Fisher
Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, California.
Demosthenes
D
Pappagianis
D016000:Cluster Analysis; D003045:Coccidioides / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014589:Utah
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer
Coccidioides immitis; Coccidioides posadasii; DNA; ITS; PCR; polymorphism