Methylation of CpG island of p14(ARK), p15(INK4b) and p16(INK4a) genes in coke oven workers.
To detect the blood genomic DNA methylation in coke oven workers and find a possible early screening index for occupational lung cancer, 74 coke oven workers as the exposed group and 47 water pump workers as the controls were surveyed, and urine samples and peripheral blood mononuclear cells (PBMCs) were collected. Airborne benzo[a]pyrene (B[a]P) levels in workplace and urinary 1-hydroxypyrene (1-OH-Py) levels were determined by high-performance liquid chromatography. DNA damage of PBMCs and the p14(ARK), p15(INK4b) and p16(INK4a) gene CpG island methylation in the promoter region were detected by comet assay and methylation-specific polymerase chain reaction techniques, respectively. Results show that compared with the controls, concentration of airborne B[a]Ps was elevated in the coke plant, and urinary 1-OH-Py's level and DNA olive tail moment in comet assay were significantly increased in the coke oven workers, and p14(ARK), p15(INK4b) and p16(INK4a) gene methylation rates were also significantly increased. With the working years and urinary 1-OH-Py's level, the rates of p14(ARK) and p16(INK4a) gene methylation were significantly increased while that of p15(INK4b) gene methylation displayed no statistical change. We conclude that PBMCs' p14(ARK) and p16(INK4a) gene methylation may be used for screening and warning lung cancer in coke oven workers.
Department of Environmental Health, Shanxi Medical University, Taiyuan, China zhhm_108@163.com.
H
H
Zhang
Center of Disease Control and Prevention, Taiyuan Iron and Steel Company, Taiyuan, China.
X
X
Li
Department of Preventive Medicine, Hebei North University, Zhangjiakou, China.
L
L
Ge
Department of Occupational Health, Shanxi Medical University, Taiyuan, China.
J
J
Yang
Center of Disease Control and Prevention, Taiyuan Iron and Steel Company, Taiyuan, China.
J
J
Sun
Department of Occupational Health, Shanxi Medical University, Taiyuan, China.
Q
Q
Niu
D000328:Adult; D000395:Air Pollutants, Occupational / Q000032:analysis*; D001564:Benzo(a)pyrene / Q000032:analysis*; D003077:Coke; D018899:CpG Islands; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D004249:DNA Damage; D004784:Environmental Monitoring; D006801:Humans; D008297:Male; D008745:Methylation; D016273:Occupational Exposure / Q000032:analysis*; D011721:Pyrenes / Q000652:urine; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics*
D000395:Air Pollutants, Occupational; D003077:Coke; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D011721:Pyrenes; D025803:Tumor Suppressor Protein p14ARF; D001564:Benzo(a)pyrene; C033146:1-hydroxypyrene
CpG island; DNA damage; Polycyclic aromatic hydrocarbons; benzo[a]pyrene; genomic DNA methylation; lung cancer