Sequential hydrothermal fractionation of yeast Cryptococcus curvatus biomass.
A sequential hydrothermal liquefaction (SEQHTL) process was evaluated in this work for fractionating different component of yeast biomass. Sugar and protein were separated first at a lower temperature, and the remaining biomass was then converted to bio-oil at a higher temperature. The separated aqueous products were investigated to be recycled as a carbon and nitrogen sources for the yeast culture. In the first step of SEQHTL, the temperature effect on the yield of sugar/protein and inhibitory compounds (acetic acid and 5-hydroxymethyl furfural (5HMF)) was investigated. The highest yields of sugar and protein and a minimal level of inhibitory compounds were obtained at 180°C. At the second step of SEQHTL, the highest bio-oil yield was achieved at 240°C. In comparison to the one-step hydrothermal liquefaction process, SEQHTL produced a higher quality bio-oil with higher fatty acid and lower nitrogen contents.
Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, United States.
Chao
C
Miao
Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, United States.
Moumita
M
Chakraborty
Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, United States.
Tao
T
Dong
Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, United States.
Xiaochen
X
Yu
Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, United States.
Zhanyou
Z
Chi
Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, United States. Electronic address: chens@wsu.edu.
Shulin
S
Chen
D019342:Acetic Acid / Q000378:metabolism; D056804:Biofuels / Q000382:microbiology; D018533:Biomass; D002606:Charcoal / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D003454:Cryptococcus / Q000378:metabolism*; D005410:Flame Ionization; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D013696:Temperature; D014867:Water / Q000737:chemistry*
D056804:Biofuels; C540010:biochar; D014867:Water; D002606:Charcoal; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; D019342:Acetic Acid
Bio-oil; Cryptococcus curvatus; Hydrothermal liquefaction; Yeast