Serum hepcidin concentrations correlate with ferritin in patients with inflammatory bowel disease.
OBJECTIVE
Anemia is a frequent complication of inflammatory bowel disease (IBD). Hepcidin, a key mediator in this anemia, is up-regulated by high iron levels and inflammation, and serum levels are elevated in IBD. However, the extent of inflammatory activity and iron deficiency for the regulation of hepcidin is not known. This study aimed to evaluate serum hepcidin levels in anemic and non-anemic IBD patients, with iron or non-iron deficiency, and active or inactive disease.


METHODS
This retrospective, observational study analyzed serum hepcidin levels from 247 patients with IBD (130 Crohn's patients and 117 with ulcerative colitis) recruited at Swiss Inflammatory Bowel Disease Cohort Study centers. Patients were divided into 5 different groups using criteria of active and inactive diseases (C-reactive protein, and CDAI/MTWAI=disease activity-index), anemia (hemoglobin) and iron deficiency (ferritin) and compared to healthy controls with no signs of anemia and normal ferritin levels. Hepcidin was measured using enzyme-linked immunosorbent assay.


RESULTS
Independent of inflammatory activity, all patients with decreased ferritin (<30μg/L) had significantly lower hepcidin levels when compared to patients and healthy controls having normal ferritin (>30μg/L). A significant correlation between serum ferritin levels and serum hepcidin was found (Spearman's Rho=0.491; p<0.001). A backward multi-linear stepwise regression analysis showed that only ferritin, and none of the inflammatory markers or age and sex correlated significantly (p=0.005) with hepcidin.


CONCLUSIONS
This retrospective analysis suggests that iron deficiency is the key trigger for hepcidin regulation in IBD patients with anemia.
Department of Gastroenterology and Hepatology, University Hospital Basel, Basel, Switzerland.
Ingo
I
Mecklenburg
Department of Gastroenterology and Hepatology, University Hospital Basel, Basel, Switzerland.
Diana
D
Reznik
Department of Gastroenterology and Hepatology, University Hospital Basel, Basel, Switzerland; University of Applied Sciences Northwestern Switzerland (FHNW), Institute of Chemistry and Bioanalytics, Muttenz, Switzerland.
Elizaveta
E
Fasler-Kan
Department of Gastroenterology and Hepatology, University Hospital Basel, Basel, Switzerland.
Jürgen
J
Drewe
Department of Gastroenterology and Hepatology, University Hospital Basel, Basel, Switzerland.
Christoph
C
Beglinger
Department of Gastroenterology and Hepatology, University Hospital Basel, Basel, Switzerland. Electronic address: petr.hruz@usb.ch.
Petr
P
Hruz
D000293:Adolescent; D000328:Adult; D000368:Aged; D018798:Anemia, Iron-Deficiency / Q000097:blood* / Q000209:etiology; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000097:blood* / Q000150:complications; D003424:Crohn Disease / Q000097:blood* / Q000150:complications; D005260:Female; D005293:Ferritins / Q000097:blood; D006454:Hemoglobins / Q000378:metabolism; D064451:Hepcidins / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012720:Severity of Illness Index; D055815:Young Adult
D006454:Hemoglobins; D064451:Hepcidins; D002097:C-Reactive Protein; D005293:Ferritins
Anemia; Crohn's disease; Hepcidin; Inflammatory bowel disease; Ulcerative colitis