Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients.
Women infected with human papillomavirus (HPV) are at a higher risk of developing cervical lesions. In the current study, self and clinician-collected vaginal and cervical samples from women were processed to detect HPV DNA using polymerase chain reaction (PCR) with PGMY09/11 primers. HPV genotypes were determined using type-specific PCR. HPV DNA detection showed good concordance between self and clinician-collected samples (84.6%; kappa = 0.72). HPV infection was found in 30% women and genotyping was more concordant among high-risk HPV (HR-HPV) than low-risk HPV (HR-HPV). HPV16 was the most frequently detected among the HR-HPV types. LR-HPV was detected at a higher frequency in self-collected; however, HR-HPV types were more frequently identified in clinician-collected samples than in self-collected samples. HPV infections of multiple types were detected in 20.5% of clinician-collected samples and 15.5% of self-collected samples. In this study, we demonstrated that the HPV DNA detection rate in self-collected samples has good agreement with that of clinician-collected samples. Self-collected sampling, as a primary prevention strategy in countries with few resources, could be effective for identifying cases of HR-HPV, being more acceptable. The use of this method would enhance the coverage of screening programs for cervical cancer.
Laboratório de Imunologia e Biologia Molecular de Vírus, Centro de Ciências Biológicas e da Saúde.
Karla Lopes Mandu de
KL
Campos
Laboratório de Imunologia e Biologia Molecular de Vírus, Centro de Ciências Biológicas e da Saúde.
Ana Paula
AP
Machado
Laboratório de Imunologia e Biologia Molecular de Vírus, Centro de Ciências Biológicas e da Saúde.
Flávia Gatto de
FG
Almeida
Laboratório de Imunologia e Biologia Molecular de Vírus, Centro de Ciências Biológicas e da Saúde.
Camila Mareti
CM
Bonin
Laboratório de Imunologia e Biologia Molecular de Vírus, Centro de Ciências Biológicas e da Saúde.
Thiago Theodoro Martins
TT
Prata
Laboratório de Imunologia e Biologia Molecular de Vírus, Centro de Ciências Biológicas e da Saúde.
Larissa Zatorre
LZ
Almeida
Laboratório de Imunologia e Biologia Molecular de Vírus, Centro de Ciências Biológicas e da Saúde.
Cacilda Tezelli Junqueira
CT
Padovani
Laboratório de Imunologia e Biologia Molecular de Vírus, Centro de Ciências Biológicas e da Saúde.
Alda Maria Teixeira
AM
Ferreira
Laboratório de Imunologia e Biologia Molecular de Vírus, Centro de Ciências Biológicas e da Saúde.
Carlos Eurico Dos Santos
CE
Fernandes
Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
Inês Aparecida
IA
Tozetti
D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D012648:Self Care / Q000379:methods; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D055815:Young Adult
D004279:DNA, Viral