A novel fully water-soluble Cu(I) probe for fluorescence live cell imaging.
A new Cu(I) probe (OBEP-CS1), bearing an alkyl-pyridinium moiety to get complete water solubility, was synthesized. Fluorescence titrations support copper binding and high selectivity in a water solution whereas formation of aggregates was excluded by DOSY experiments. The detection capability for intracellular Cu(I), especially localized in mitochondria, was demonstrated in differentiated neuroblastoma cells by confocal microscopy.
Institute of Biostructure and Bioimaging, National Research Council, Viale Andrea Doria, Catania 95125, Italy.
Maria Laura
ML
Giuffrida
Enrico
E
Rizzarelli
Gaetano A
GA
Tomaselli
Cristina
C
Satriano
Giuseppe
G
Trusso Sfrazzetto
D045744:Cell Line, Tumor; D003300:Copper / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000737:chemistry; D061848:Optical Imaging; D011726:Pyridinium Compounds / Q000737:chemistry*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D014867:Water; D003300:Copper