Molecular cloning of α-2-macroglobulin from hemocytes of common periwinkle Littorina littorea.
We report the sequence of the proteinase inhibitor with a wide inhibition spectrum, α-2-macroglobulin (α2M), belonging to the thioester superfamily of proteins. This is the first α2M sequence from coenogastropod prosobranch snails. The full-length cDNA was cloned by RACE method, spans 7897 bp and contains an open reading frame of 5460 bp. The ORF encodes a protein of 1819 amino acids. The deduced mature protein contains 1795 amino acids with a molecular weight of 200 kDa and isoelectric point of 5.00. Littorina littorea α2M bears 4 conserved α2M domains and one internal thioester. Phylogenetic analysis showed that the sequence forms well supported cluster with Mollusca species and other representatives of Lophotrochozoa.
Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences (IEPhB RAS), St-Petersburg, Russia.
Elena A
EA
Borisova
Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences (IEPhB RAS), St-Petersburg, Russia. Electronic address: agorbushin@gmail.com.
Alexander M
AM
Gorbushin
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D006434:Hemocytes / Q000378:metabolism*; D007113:Immunity, Innate / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012908:Snails / Q000235:genetics* / Q000276:immunology; D000511:alpha-Macroglobulins / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary; D000511:alpha-Macroglobulins
Coenogastropoda; Hemocytes; Innate immunity; Mollusca; Proteinase inhibitors