Recombinant human BMP-2 enhances osteogenesis of demineralized bone matrix in experimental mastoid obliteration.
CONCLUSIONS
From the results, recombinant human bone morphogenic protein 2 (rhBMP-2) activated demineralized bone matrix (DBM) for the enhancement of bone regeneration. These results might provide a basis for the clinical application of BMP-2 in mastoid obliteration.


OBJECTIVE
The purpose of this study was to evaluate the enhanced osteogenesis of rhBMP-2-loaded DBM using a gelatin sponge in the mastoid obliteration model.


METHODS
The bulla obliteration was done using rhBMP-2 (0.075 mg/ml)/DBM in experimental group I (n = 7) and rhBMP-2 (0.375 mg/ml)/DBM in experimental group II (n = 7). In the control group (n = 7), the bullae were obliterated using PBS/DBM. To assess the active mineralization of new bone formation, each group received intravenous calcein blue at 4 weeks, oxytetracycline hydrochloride at 8 weeks, and alizarin red at 10 weeks. The animals in each group were sacrificed 12 weeks post-surgery. Osteogenesis was evaluated by in vivo CT and histological observation.


RESULTS
The largest amount of bone had formed in experimental group II compared with other groups according to CT and histopathological findings. Histomorphometric analysis showed that there were significant differences between each group. Confocal microscopic findings revealed that three distinct colors that corresponded to sequential osteogenesis were observed in group II. However, poor sequential osteogenesis was observed in the control group.
Department of Bio New Drug Development, Chosun University , Gwangju.
Yoon Seok
YS
Jang
Cheol Hee
CH
Choi
Yong Beom
YB
Cho
Myung-Koo
MK
Kang
Chul Ho
CH
Jang
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D004195:Disease Models, Animal; D008297:Male; D008416:Mastoid / Q000000981:diagnostic imaging / Q000601:surgery*; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D014433:Tympanoplasty; D055114:X-Ray Microtomography
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2
Bone morphogenic protein; bone regeneration; osteogenesis