Immobilization of penicillin G acylase on paramagnetic aldehyde-functionalized mesostructured cellular foams.
Paramagnetic aldehyde-functionalized mesostructured cellular foams (PAMCFs), synthesized by grafting 3-aminopropyltriethoxysilane modified Fe3O4 (NH2-Fe3O4) nanoparticles with larger particle size than the window pore size of MCFs on the outer surface of aldehyde-functionalized mesostructured cellular foams (AMCFs), were investigated as efficient supports for immobilization of penicillin G acylase (PGA). The results show that NH2-Fe3O4 nanoparticles were successfully grafted on the outer surface of AMCFs and PGA molecules were mainly immobilized covalently on the inner surface of PAMCFs, which was because amino groups of NH2-Fe3O4 nanoparticles or PGA molecules reacted with aldehyde groups of AMCFs or PAMCFs to form imine bonds. PGA/PAMCFs-15 showed a rather high initial activity of 9563Ug(-1) and retained 89.1% of its initial activity after recycled for 10 times. PGA/PAMCFs are easily recycled by magnetic field in order to replace tedious separation of high-speed centrifugation for mesoporous materials.
Key Laboratory for Advanced Materials, Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237, PR China.
Ling
L
Yang
Key Laboratory for Advanced Materials, Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237, PR China.
Zhenyuan
Z
Gao
Key Laboratory for Advanced Materials, Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: ylguo@ecust.edu.cn.
Yanglong
Y
Guo
Key Laboratory for Advanced Materials, Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237, PR China.
Wangcheng
W
Zhan
Key Laboratory for Advanced Materials, Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237, PR China.
Yun
Y
Guo
Key Laboratory for Advanced Materials, Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237, PR China.
Yunsong
Y
Wang
Key Laboratory for Advanced Materials, Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: gzhlu@ecust.edu.cn.
Guanzhong
G
Lu
D000447:Aldehydes / Q000737:chemistry; D055162:Biocatalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D010399:Penicillin Amidase / Q000737:chemistry* / Q000378:metabolism*; D016062:Porosity; D011437:Propylamines; D053838:Scattering, Small Angle; D012821:Silanes; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
D000447:Aldehydes; D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D011437:Propylamines; D012821:Silanes; D010399:Penicillin Amidase; C477625:amino-propyl-triethoxysilane
Aldehyde groups; Immobilization; Mesostructured cellular foams; Paramagnetic; Penicillin G acylase