Study of chemical stability of lemon oil components in sodium caseinate-lactose glycoconjugate-stabilized oil-in-water emulsions using solid-phase microextraction-gas chromatography.
A headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC/MS) method was developed to quantify lemon oil components and their degradation products in oil-in-water (O/W) emulsions prepared with sodium caseinate-heated-lactose (NaC-T + Lact) glycoconjugates as wall materials at two pH values (3.0 and 6.8). NaC-T + Lact conjugates had a significantly lower solubility at both pHs. Hydrolysis prior to glycation enhanced the solubility of glycoconjugates. Glycation with lactose did not improve the emulsion activity of NaC, while caseinate glycoconjugates showed much stronger antioxidant activity than the NaC-control sample. This might be due to the presence of melanoidins formed between the sugar and amino acid compounds as supported by the increase in browning intensity. Among the SPME-fibres tested, carboxen/polydimethylsiloxane (CAR/PDMS) provided better results in terms of sensitivity and selectivity for oil lemon components and their degradation products. Storage studies of these emulsions demonstrated that glycated NaC-T + Lact showed protection against peroxidation compared to the control. However, acidic pH conditions altered their stability over storage time. The major off-flavor components (α-terpineol and carvone) were inhibited in emulsions stabilized with glycated NaC, particularly at pH 6.8. The use of NaC-T + Lact conjugates showed improved encapsulation efficiency and stability and could be used as potential food ingredient-emulsifiers for stabilising citrus oils against oxidative degradation in food and beverage applications.
Food Research and Development Centre, Agriculture and Agri-Food Canada, St-Hyacinthe, Quebec J2S 8E3, Canada. patrick.fustier@agr.gc.ca.
Hassan
H
Sabik
Allaoua
A
Achouri
Maria
M
Alfaro
Marylène
M
Pelletier
Denis
D
Belanger
Michel
M
Britten
Patrick
P
Fustier
D002138:Calibration; D002364:Caseins / Q000737:chemistry*; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006001:Glycoconjugates / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D039821:Monoterpenes / Q000737:chemistry; D010084:Oxidation-Reduction; D010938:Plant Oils / Q000737:chemistry*; D052617:Solid Phase Microextraction
D002364:Caseins; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D004655:Emulsions; D006001:Glycoconjugates; D039821:Monoterpenes; D010938:Plant Oils; C016775:alpha-terpineol; C006923:carvone; C501365:lemon oil