MicroRNA-19b targets Mfn1 to inhibit Mfn1-induced apoptosis in osteosarcoma cells.
Accumulative evidence has confirmed that, miR-17-92, a typical polycistronic mRNA cluster, was up-regulated in various solid tumors, and play an important role in the occurrence and development progress of tumors. In our study, we detected the six members of miR-17-92 cluster in osteosarcoma cell line, finding that the expression of miR-17 and miR-19b was up-regulated significantly. Further studies have found that Mfn1 was one of the target genes of miR-19b and the transcription and expression level of Mfn1 were down-regulated by miR-19b. MTS, flow cytometry, TUNEL-DAPI, Annexin V-FITC and transwell assay demonstrated that Mfn1 significantly blocked the cell cycle, promoted apoptosis and inhibited proliferation and invasion of osteosarcoma cells. Whereas, miR-19b targets 3'UTR sequences of Mfn1 genes inhibit the expression of Mfn1, thus inhibited Mfn1 triggered anti-cancer effect. Taken together, miR-19b functions by targeting Mfn1 reduce the protein expression level, thus provides a novel target to understand the molecular biology and genetics mechanisms of occurrence and development of osteosarcoma, contributing to the diagnosis and therapy of osteosarcoma.
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Li
F S
FS
Wang
Z Y
ZY
Wu
J L
JL
Lin
W B
WB
Lan
J H
JH
Lin
D020413:3' Untranslated Regions; D017209:Apoptosis; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D020558:GTP Phosphohydrolases / Q000235:genetics* / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D033681:Mitochondrial Membrane Transport Proteins / Q000235:genetics* / Q000502:physiology; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology* / Q000556:secondary
D020413:3' Untranslated Regions; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D033681:Mitochondrial Membrane Transport Proteins; D020558:GTP Phosphohydrolases; C470017:Mfn1 protein, human