Characterization of a micro-roughened TiO2/ZrO2 coating: mechanical properties and HBMSC responses in vitro.
Previous studies have shown that using ZrO2 as a second phase to bioceramics can significantly increase the bonding strength of plasma-sprayed composite material. In the present study, micro-roughened titanium dioxide/zirconia (TiO2/ZrO2) (30 wt% ZrO2) coating and TiO2 coating were plasma-sprayed onto Ti plates. The micro-structural characteristics and mechanical properties of both coatings were investigated. Furthermore, the biological behavior and osteogenic differentiation of human bone marrow mesenchymal stem cells (HBMSCs) on both TiO2/ZrO2 and TiO2 coatings were compared. The results indicated that the shear bond strength and microhardness of TiO2/ZrO2 coating were statistically higher than those of TiO2 coating. Scanning electron microscope observation revealed that more irregularly shaped protuberances and denser pores were formed on the surface of TiO2/ZrO2 coating compared with those of TiO2 coating. Further comparative analysis of HBMSC proliferation and osteogenic differentiation on both coatings showed that significantly higher cellular alkaline phosphatase activity and expression levels of Runx2 and Osterix at day 10 after osteogenic culture were found on TiO2/ZrO2 coating compared with TiO2 coating, while no statistically significant difference in cell proliferation and extracellular calcium deposition was observed. The present study suggests that TiO2/ZrO2 coating may be favorable for dental implant applications.
Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
Jiawen
J
Si
Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
Jianjun
J
Zhang
Shanghai United Stem Cell Biotechnology Co. Ltd, Shanghai 200333, China.
Sha
S
Liu
Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
Wenbin
W
Zhang
Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
Dedong
D
Yu
Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
Xudong
X
Wang
Shanghai United Stem Cell Biotechnology Co. Ltd, Shanghai 200333, China Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China maxillofacsurg@163.com guolihe@cell-star.com.cn.
Lihe
L
Guo
Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China maxillofacsurg@163.com guolihe@cell-star.com.cn.
Steve G F
SG
Shen
D001483:Base Sequence; D001672:Biocompatible Materials; D002454:Cell Differentiation; D002478:Cells, Cultured; D017931:DNA Primers; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008422:Materials Testing; D010012:Osteogenesis; D060888:Real-Time Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
D001672:Biocompatible Materials; D017931:DNA Primers; C009495:titanium dioxide; D015040:Zirconium; D014025:Titanium; C028541:zirconium oxide
human bone mesenchymal stem cell; mechanical property; osteogenesis; plasma spraying; titanium dioxide; zirconia