Extract-filter-shoot liquid chromatography with mass spectrometry for the analysis of vitamin D2 in a powdered supplement capsule and standard reference material 3280.
An "extract-filter-shoot" method for the analysis of vitamin D2, ergocalciferol, in a dry powdered dietary supplement capsule containing rice flour excipient and in a National Institute of Standards and Technology standard reference material 3280 is reported. Quantification of vitamin D2 was done by atmospheric pressure chemical ionization mass spectrometry using selected ion monitoring, two transitions of selected reaction monitoring, and extracted ion chromatograms from full scans. UV detection was used for the quantification of Vitamin D2 in the dry powder capsule, whereas interfering species rendered UV detection unreliable for standard reference material 3280. Average values for standard reference material 3280 ranged from 8.27 ± 0.58 to 8.33 ± 0.57 μg/g using internal standard calibration and response factor approaches, compared to the previous National Institute of Standards and Technology internal value for vitamin D2 of 8.78 ± 0.11 μg/g, and the recently updated reference value of 8.6 ± 2.6 μg/g. The powdered supplement capsule was found to contain 28.19 ± 0.35 to 28.67 ± 0.90 μg/capsule for a capsule labeled to contain 25.00 μg. The triacylglycerol composition of the rice flour excipient in the powdered supplement capsule determined by atmospheric pressure chemical ionization mass spectrometry is also reported.
Beltsville Human Nutrition Research Center, Food Composition and Methods Development Lab, Agricultural Research Service, USDA, Beltsville, MD, USA.
William Craig
WC
Byrdwell
D002138:Calibration; D002214:Capsules / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002762:Cholecalciferol / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements; D004075:Diglycerides / Q000737:chemistry; D004872:Ergocalciferols / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D013058:Mass Spectrometry / Q000379:methods*; D012275:Oryza; D012015:Reference Standards; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014280:Triglycerides / Q000737:chemistry; D014466:Ultraviolet Rays
D002214:Capsules; D004075:Diglycerides; D004872:Ergocalciferols; D012997:Solvents; D014280:Triglycerides; D002762:Cholecalciferol
Cholecalciferol; Ergocalciferol; Vitamin D