Live-cell reporters for fluorescence imaging.
Advances in the development of new fluorescent reporters and imaging techniques have revolutionized our ability to directly visualize biological processes in living systems. Real-time analysis of protein localization, dynamics, and interactions has been made possible by site-specific protein labeling with custom designed probes. This review outlines some of the most recent advances in the design and application of live-cell imaging probes, with a particular focus on SNAP-tag technology. Specific examples illustrating applications in superresolution and single-molecule imaging, protein trafficking and recycling, and protein-protein interactions are presented.
New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938, USA. Electronic address: correa@neb.com.
Ivan R
IR
Corrêa
D000818:Animals; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000032:analysis*; D006801:Humans; D057054:Molecular Imaging; D011485:Protein Binding; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
D005456:Fluorescent Dyes; D011506:Proteins