Nonlinear optical 3-dimensional method for quantifying atherosclerosis burden.
From the Microscopy and Dynamic Imaging Unit (S.A.S., A.M.S.-B.), Department of Vascular Biology and Inflammation (N.M.-B., V.E., J.M.R.), and Department of Atherothrombosis and Imaging (L.J.J.-B.), Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain; Cardiology Department, Hospital de la Princesa, Madrid, Spain (L.J.J.-B.); and Department of Cancer Biology, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Madrid, Spain (M.R.C.). susanchez@udec.cl mcampanero@iib.uam.es jmredondo@cnic.es.
Susana A
SA
Sánchez
From the Microscopy and Dynamic Imaging Unit (S.A.S., A.M.S.-B.), Department of Vascular Biology and Inflammation (N.M.-B., V.E., J.M.R.), and Department of Atherothrombosis and Imaging (L.J.J.-B.), Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain; Cardiology Department, Hospital de la Princesa, Madrid, Spain (L.J.J.-B.); and Department of Cancer Biology, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Madrid, Spain (M.R.C.).
Nerea
N
Méndez-Barbero
From the Microscopy and Dynamic Imaging Unit (S.A.S., A.M.S.-B.), Department of Vascular Biology and Inflammation (N.M.-B., V.E., J.M.R.), and Department of Atherothrombosis and Imaging (L.J.J.-B.), Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain; Cardiology Department, Hospital de la Princesa, Madrid, Spain (L.J.J.-B.); and Department of Cancer Biology, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Madrid, Spain (M.R.C.).
Antonio M
AM
Santos-Beneit
From the Microscopy and Dynamic Imaging Unit (S.A.S., A.M.S.-B.), Department of Vascular Biology and Inflammation (N.M.-B., V.E., J.M.R.), and Department of Atherothrombosis and Imaging (L.J.J.-B.), Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain; Cardiology Department, Hospital de la Princesa, Madrid, Spain (L.J.J.-B.); and Department of Cancer Biology, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Madrid, Spain (M.R.C.).
Vanesa
V
Esteban
From the Microscopy and Dynamic Imaging Unit (S.A.S., A.M.S.-B.), Department of Vascular Biology and Inflammation (N.M.-B., V.E., J.M.R.), and Department of Atherothrombosis and Imaging (L.J.J.-B.), Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain; Cardiology Department, Hospital de la Princesa, Madrid, Spain (L.J.J.-B.); and Department of Cancer Biology, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Madrid, Spain (M.R.C.).
Luis J
LJ
Jiménez-Borreguero
From the Microscopy and Dynamic Imaging Unit (S.A.S., A.M.S.-B.), Department of Vascular Biology and Inflammation (N.M.-B., V.E., J.M.R.), and Department of Atherothrombosis and Imaging (L.J.J.-B.), Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain; Cardiology Department, Hospital de la Princesa, Madrid, Spain (L.J.J.-B.); and Department of Cancer Biology, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Madrid, Spain (M.R.C.). susanchez@udec.cl mcampanero@iib.uam.es jmredondo@cnic.es.
Miguel R
MR
Campanero
From the Microscopy and Dynamic Imaging Unit (S.A.S., A.M.S.-B.), Department of Vascular Biology and Inflammation (N.M.-B., V.E., J.M.R.), and Department of Atherothrombosis and Imaging (L.J.J.-B.), Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain; Cardiology Department, Hospital de la Princesa, Madrid, Spain (L.J.J.-B.); and Department of Cancer Biology, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Madrid, Spain (M.R.C.). susanchez@udec.cl mcampanero@iib.uam.es jmredondo@cnic.es.
Juan Miguel
JM
Redondo
D000818:Animals; D001013:Aorta, Thoracic; D050197:Atherosclerosis / Q000175:diagnosis*; D004396:Coloring Agents; D003937:Diagnosis, Differential; D004195:Disease Models, Animal; D007089:Image Enhancement / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D017711:Nonlinear Dynamics; D058226:Plaque, Atherosclerotic / Q000175:diagnosis*; D015203:Reproducibility of Results; D012720:Severity of Illness Index
D004396:Coloring Agents
2-photon excitation; atherosclerosis burden; infrared pulsed laser; plaque volume; second harmonic generation