Establishment and Characterization of Fibroblast Cell Line Derived from Siberian Tiger (Panthera tigris altaica).
The Siberian tiger ear marginal tissue fibroblast cell line (STF34) from 34 samples was successfully established using primary explants technique and cell cryoconservation technology. STF34 cells were adherent, with a population doubling time of 24 h. Chromosome analysis showed that 90.2%-91.6% of cells were diploid (2n = 38). Isoenzyme analyses of lactate dehydrogenase and malate dehydrogenase showed that STF34 cells had no cross-contamination with other species. Tests for cell line contamination with bacteria, fungi, viruses, and mycoplasmas were all negative. Every index of the STF34 cell line meets all the standard quality controls of American Type Culture Collection. Not only has the germline of this important Siberian tiger species been preserved at the cell level, but also valuable material had been provided for genome, postgenome, and somacloning research.
1 Chinese Academy of Agricultural Science, Institute of Beijing Animal Science and Veterinary , Beijing, People's Republic of China .
Changqing
C
Liu
Yu
Y
Guo
Dan
D
Liu
Weijun
W
Guan
Yuehui
Y
Ma