Simple and rapid detection of Salmonella by direct PCR amplification of gene fimW.
This study established a simple method of specifically detecting Salmonella species by amplifying fimW gene, which was involved in regulating Salmonella type I fimbriae expression. A pair of primers was designed to target and discriminate the 68 Salmonella strains of 23 Salmonella serovars available to us from 12 non-Salmonella strains of five different kinds of bacteria by polymerase chain reaction (PCR) amplification. Results showed that specific DNA fragment with an expected size of 477 bp was successfully amplified from all Salmonella serovars, while no target band was detected in non-Salmonella species. The sensitivity of this PCR-amplifying system reached to 1 pg DNA chromosome and 10(2) cfu of Salmonella enteritis strain CMCC(B) 50336. The above results demonstrated the method as a simple, sensitive, and specific way for Salmonella detection.
College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
Jiang-ying
JY
Zhang
Li-wei
LW
Dong
Qian
Q
Ren
Xiao-zhou
XZ
Wang
Yi
Y
Yang
Wen
W
Zhou
Chun-hong
CH
Zhu
Xia
X
Meng
Guo-qiang
GQ
Zhu
D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005784:Gene Amplification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D017931:DNA Primers