A protective chimeric antibody to tick-borne encephalitis virus.
The efficiency of several mouse monoclonal antibodies (mAbs) specific to the tick-borne encephalitis virus (TBEV) glycoprotein E in post-exposure prophylaxis was assessed, and mAb14D5 was shown to be the most active of all those studied. It was proven that the hybridoma cell line 14D5 produced one immunoglobulin H chain and two L chains. They were used to construct chimeric antibodies ch14D5a and ch14D5b, the affinity constants of which were 2.6 × 10(10)M(-1) and 1.0 × 10(7)M(-1), respectively, according to the SPR-based ProteOn biosensor assay. The neutralization index (IC50) of ch14D5a was 0.04 μg/ml in the focus reduction neutralization test. In in vivo experiments, ch14D5a at a dose of 10 μg/mouse resulted in a 100% survival of the mice infected with 240 LD50 of TBEV. This chimeric antibody is promising for further development of prevention and therapeutic drugs against TBEV.
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, prosp. Lavrentieva 8, Novosibirsk 630090, Russia. Electronic address: ivan_baykov@mail.ru.
Ivan K
IK
Baykov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, prosp. Lavrentieva 8, Novosibirsk 630090, Russia. Electronic address: guterus@gmail.com.
Andrey L
AL
Matveev
Federal State Unitary Company "Microgen Scientific Industrial Company for Immunobiological Medicines" of the Health Ministry of The Russian Federation, Branch in Tomsk, ul. Ivanovskogo 8, Tomsk 634040, Russia. Electronic address: stronin@virion.tomsknet.ru.
Oleg V
OV
Stronin
Federal State Research Institution State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region 630559, Russia. Electronic address: ryzhik@vector.nsc.ru.
Alexander B
AB
Ryzhikov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, prosp. Lavrentieva 8, Novosibirsk 630090, Russia. Electronic address: vam@niboch.nsc.ru.
Leonid E
LE
Matveev
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, prosp. Lavrentieva 8, Novosibirsk 630090, Russia. Electronic address: kassakinm@gmail.com.
Marat F
MF
Kasakin
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, prosp. Lavrentieva 8, Novosibirsk 630090, Russia. Electronic address: richter@niboch.nsc.ru.
Vladimir A
VA
Richter
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, prosp. Lavrentieva 8, Novosibirsk 630090, Russia. Electronic address: tikunova@niboch.nsc.ru.
Nina V
NV
Tikunova
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D058846:Antibodies, Monoclonal, Murine-Derived / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology; D004675:Encephalitis, Tick-Borne / Q000517:prevention & control*; D006825:Hybridomas; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D056990:Post-Exposure Prophylaxis; D014759:Viral Envelope Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus
Affinity constant; Chimeric antibody; In vivo protection; Post-exposure prophylaxis; Tick-borne encephalitis virus; Virus neutralization