In vivo electroporation of adult mouse sensory neurons for studying peripheral axon regeneration.
Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain- and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.
Department of Orthopaedic Surgery, The Johns Hopkins University, Baltimore, MD, USA.
Saijilafu
Bo-Yin
BY
Zhang
Feng-Quan
FQ
Zhou
D000818:Animals; D001369:Axons / Q000502:physiology*; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D018274:Electroporation / Q000191:economics / Q000379:methods*; D005260:Female; D005727:Ganglia, Spinal / Q000502:physiology / Q000601:surgery; D051379:Mice; D008845:Microinjections / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D009416:Nerve Regeneration; D019635:Neurosurgical Procedures / Q000379:methods; D020320:Oligoribonucleotides, Antisense / Q000008:administration & dosage; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D012584:Sciatic Nerve / Q000502:physiology / Q000601:surgery; D011984:Sensory Receptor Cells / Q000502:physiology*; D014162:Transfection / Q000191:economics / Q000379:methods*
D020320:Oligoribonucleotides, Antisense; D004247:DNA