Effect of zirconium nitride physical vapor deposition coating on preosteoblast cell adhesion and proliferation onto titanium screws.
BACKGROUND
Titanium has long been used to produce dental implants. Problems related to its manufacturing, casting, welding, and ceramic application for dental prostheses still limit its use, which highlights the need for technologic improvements. The aim of this in vitro study was to evaluate the biologic performance of titanium dental implants coated with zirconium nitride in a murine preosteoblast cellular model.


OBJECTIVE
The purpose of this study was to evaluate the chemical and morphologic characteristics of titanium implants coated with zirconium nitride by means of physical vapor deposition.


METHODS
Chemical and morphologic characterizations were performed by scanning electron microscopy and energy dispersive x-ray spectroscopy, and the bioactivity of the implants was evaluated by cell-counting experiments.


RESULTS
Scanning electron microscopy and energy dispersive x-ray spectroscopy analysis found that physical vapor deposition was effective in covering titanium surfaces with zirconium nitride. Murine MC-3T3 preosteoblasts were seeded onto titanium-coated and zirconium nitride-coated screws to evaluate their adhesion and proliferation. These experiments found a significantly higher number of cells adhering and spreading onto zirconium nitride-coated surfaces (P<.05) after 24 hours; after 7 days, both titanium and zirconium nitride surfaces were completely covered with MC-3T3 cells.


CONCLUSIONS
Analysis of these data indicates that the proposed zirconium nitride coating of titanium implants could make the surface of the titanium more bioactive than uncoated titanium surfaces.
Fellow, Innovative Research Laboratory for Wound Healing, Health Sciences Department, University of Eastern Piedmont, Novara, Italy.
Manuela
M
Rizzi
Researcher, Department of Sciences and Technological Innovation, University of Eastern Piedmont, Alessandria, Italy.
Giorgio
G
Gatti
Researcher, Dental Clinic, Health Sciences Department, University of Eastern Piedmont, Novara, Italy.
Mario
M
Migliario
Professor, Department of Sciences and Technological Innovation, University of Eastern Piedmont, Alessandria, Italy.
Leonardo
L
Marchese
Professor, Dental Clinic, Health Sciences Department, University of Eastern Piedmont, Novara, Italy.
Vincenzo
V
Rocchetti
Professor, Innovative Research Laboratory for Wound Healing, Health Sciences Department, University of Eastern Piedmont, Novara, Italy. Electronic address: filippo.reno@med.unipmn.it.
Filippo
F
Renò
D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D017672:Nitrogen Compounds / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects* / Q000648:ultrastructure; D011554:Pseudopodia / Q000648:ultrastructure; D013052:Spectrometry, X-Ray Emission; D014025:Titanium / Q000737:chemistry*; D014835:Volatilization; D015040:Zirconium / Q000494:pharmacology*
D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D003764:Dental Materials; D017672:Nitrogen Compounds; D015040:Zirconium; D014025:Titanium