The effect of Leonurus cardiaca herb extract and some of its flavonoids on mitochondrial oxidative phosphorylation in the heart.
Motherwort (Leonurus cardiaca) possesses antibacterial, antioxidant, anti-inflammatory, and analgesic activities, and is used as a complementary remedy to improve heart function and blood circulation. Since cardiovascular diseases are often associated with an alteration of mitochondria, the main producers of ATP in cardiac muscle cells, the aim of our work was to determine bioactive constituents present in motherwort aerial parts extract in ethanol and investigate their effects on the functions of cardiac mitochondria. Quantitative determination of polyphenols in L. cardiaca herb extract was performed by HPLC. Mitochondrial respiration rates were evaluated using a Clark-type oxygen electrode. Mitochondrial ROS generation was determined fluorimetrically with Amplex Red and horseradish peroxidase. The results showed that constituents (chlorogenic acid, orientin, quercetin, hyperoside, and rutin) of L. cardiaca herb extract uncouple (by 20-90 %) mitochondrial oxidation from phosphorylation, partially inhibit (by ~ 40 %) the mitochondrial respiratory chain in cases of pyruvate and malate as well as succinate oxidation, and effectively attenuate the generation of free radicals in mitochondria. Since partial uncoupling of mitochondria, respiratory inhibition, and decreased ROS production are proposed as possible mechanisms of cardioprotection, our results imply that L. cardiaca herb extract could be a useful remedy to protect cardiac muscles from the effects of pathogenic processes.
Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Jurga
J
Bernatoniene
Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Dalia M
DM
Kopustinskiene
Department of Pharmacognosy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Valdas
V
Jakstas
Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Daiva
D
Majiene
Laboratory of Biochemistry, Neuroscience Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania.
Rasa
R
Baniene
Department of Biochemistry, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Lolita
L
Kuršvietiene
University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Pharmacy, Department of Pharmaceutics, Brno, Czech Republic.
Ruta
R
Masteikova
Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Arunas
A
Savickas
Laboratory of Biochemistry, Neuroscience Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania.
Adolfas
A
Toleikis
Laboratory of Biochemistry, Neuroscience Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania.
Sonata
S
Trumbeckaite
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019069:Cell Respiration / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D031332:Leonurus / Q000737:chemistry*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects*; D032383:Myocytes, Cardiac / Q000187:drug effects; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols; D006861:Hydrogen Peroxide