Antibody-dependent cellular cytotoxicity and cytokine/chemokine secretion by KHYG-1 cells stably expressing FcγRIIIA.
Antibody-dependent cellular cytotoxicity (ADCC) mediated by natural killer (NK) cells is a major mechanism of tumor therapy with antibodies. NK cells not only manifest cytotoxicity but also secrete a variety of cytokines/chemokines that regulate immune responses. Using a retroviral vector, in this study we established a KHYG-1 cell line that stably expresses FcγRIIIA (CD16A). The KHYG-1/FcγRIIIA cells exerted potent antibody concentration-dependent ADCC, whereas parental KHYG-1 cells did not. In contrast, without antibody, the natural killer activity of KHYG-1/FcγRIIIA cells was less potent than that of parental KHYG-1 cells. During the course of ADCC, KHYG-1/FcγRIIIA cells secreted IFN-γ and MIP-1α dependent upon antibody concentration, but parental KHYG-1 cells did not. These results suggest that KHYG-1/FcγRIIIA cells would be useful in studies to elucidate the function of NK cells and the mechanism of ADCC.
Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki 2641, Noda, Chiba 278-8510, Japan. Electronic address: ekoba@med.u-toyama.ac.jp.
Eiji
E
Kobayashi
Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki 2641, Noda, Chiba 278-8510, Japan.
Sotaro
S
Motoi
ACTGen, Inc., 15-502 Akaho, Komagane, Nagano 399-4117, Japan.
Masahito
M
Sugiura
ACTGen, Inc., 15-502 Akaho, Komagane, Nagano 399-4117, Japan.
Masunori
M
Kajikawa
Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki 2641, Noda, Chiba 278-8510, Japan.
Shuji
S
Kojima
Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki 2641, Noda, Chiba 278-8510, Japan.
Junya
J
Kohroki
Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki 2641, Noda, Chiba 278-8510, Japan.
Yasuhiko
Y
Masuho
D000920:Antibody-Dependent Cell Cytotoxicity; D002460:Cell Line; D018925:Chemokines / Q000096:biosynthesis*; D016207:Cytokines / Q000096:biosynthesis*; D015870:Gene Expression; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism*; D017452:Receptors, IgG / Q000235:genetics* / Q000378:metabolism
D018925:Chemokines; D016207:Cytokines; C496124:FCGR3A protein, human; D017452:Receptors, IgG
ADCC; CD16A; FcγRIIIA; KHYG-1 cells; NK cells