Ochrovirga pacifica gen. nov., sp. nov., a novel agar-lytic marine bacterium of the family Flavobacteriaceae isolated from a seaweed.
A strain designated as S85(T) was isolated from a seaweed collected from coastal area of Chuuk State in Micronesia. The strain was gram-negative, rod-shaped, and non-motile and formed yellow colonies on the SWY agar (0.2 % yeast extract and 1.5 % agar in seawater) and Marine agar 2216. The strain grew at pH 5-9 (optimum, pH 8), at 15-40 °C (optimum, 25-28 °C), and with 1-9 % (w/v) NaCl (optimum, 3 %). The phylogenetic analysis based on 16S rRNA gene sequence showed that strain S85(T) was related to Lutibacter litoralis CL-TF09(T) and Maritimimonas rapanae A31(T) with 91.4 % and with 90.5 % similarity, respectively. The dominant fatty acids were iso-C15:0, iso-C15:0 3-OH and iso-C17:0 3-OH, C16:0 3-OH and summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH). The major isoprenoid quinone was MK-6. The DNA G+C content of the type strain was 34.6 mol %. The major polar lipids were phosphatidylethanolamine, an unknown glycolipid and two unknown polar lipids. Based on this polyphasic taxonomic data, strain S85(T) stands for a novel species of a new genus, and we propose the name Ochrovirga pacifica gen. nov., sp. nov. The type strain of O. pacifica is S85(T) (=KCCM 90106 =JCM 18327(T)).
Korea Institute of Ocean Science & Technology, Ansan, 426-744, Republic of Korea.
Young-Kyung
YK
Kwon
Ji Hyung
JH
Kim
Jennifer Jooyoun
JJ
Kim
Sung-Hyun
SH
Yang
Bo-Ram
BR
Ye
Soo-Jin
SJ
Heo
Jung-Ho
JH
Hyun
Zhong-Ji
ZJ
Qian
Heung-Sik
HS
Park
Do-Hyung
DH
Kang
Chulhong
C
Oh
D000362:Agar / Q000378:metabolism; D001482:Base Composition; D005227:Fatty Acids / Q000378:metabolism; D041965:Flavobacteriaceae / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D008849:Micronesia; D008969:Molecular Sequence Data; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*; D012624:Seaweed / Q000382:microbiology*
D005227:Fatty Acids; D000362:Agar