A three-dimensional multiporous fibrous scaffold fabricated with regenerated spider silk protein/poly(l-lactic acid) for tissue engineering.
An axially aligned three-dimensional (3-D) fibrous scaffold was fabricated with regenerated spider silk protein (RSSP)/poly (l-lactic acid) (PLLA) through electrospinning and post treatment. The morphology, mechanical and degradation properties of the scaffold were controlled through the weight ratio of RSSP to PLLA, the thickness of the scaffold and the treatment time. The scaffold with a weight ratio of 2:3 (RSSP:PLLA) had a nanoleaves-on-nanofibers hierarchical nanostructure; the length and thickness of the nanoleaves were about 400 and 30 nm, respectively. The holes of the scaffolds ranged from hundreds of nanometers to several microns. The scaffold showed an ideal mechanical property that it was stiff when dry, but became soft once hydrated in the culture medium. Its degradation rate was very slow in the first 2 months, and then accelerated in the following 2 months. The pH values of the degradation mediums of all the samples remained in the range of 7.40-7.12 during degradation for 6 months. It had good biocompatibility with PC 12 cells. The aligned hierarchical nanostructure could guide the directions of the axon extension. This scaffold has a potential application in Tissue Engineering and controlled release. This study provides a method to produce synthetic or natural biodegradable polymer scaffold with tailored morphology, mechanical, and degradation properties.
College of Materials and Textile Engineering, Experimental Center for Materials and Textile Engineering of Zhejiang, Jiaxing Key Lab of Medical Molecular Biology, Jiaxing University, Jiaxing, Zhejiang, 314001, People's Republic of China.
Qiaozhen
Q
Yu
Chengjun
C
Sun
D000818:Animals; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D016716:PC12 Cells; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D013112:Spiders; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)
electrospinning; fiber scaffold; hierarchical nanostructures; spider silk protein; tissue engineering