Modeling of process parameters for enhanced production of coenzyme Q10 from Rhodotorula glutinis.
Coenzyme Q10 (CoQ10) plays an indispensable role in ATP generation through oxidative phosphorylation and helps in scavenging superoxides generated during electron transfer reactions. It finds extensive applications specifically related to oxidative damage and metabolic dysfunctions. This article reports the use of a statistical approach to optimize the concentration of key variables for the enhanced production of CoQ10 by Rhodotorula glutinis in a lab-scale fermenter. The culture conditions that promote optimum growth and CoQ10 production were optimized and the interaction of significant variables para-hydroxybenzoic acid (PHB, 819.34 mg/L) and soybean oil (7.78% [v/v]) was studied using response surface methodology (RSM). CoQ10 production increased considerably from 10 mg/L (in control) to 39.2 mg/L in batch mode with RSM-optimized precursor concentration. In the fed-batch mode, PHB and soybean oil feeding strategy enhanced CoQ10 production to 78.2 mg/L.
a Centre for Biotechnology , Anna University , Chennai , India.
Palanisamy Athiyaman
PA
Balakumaran
Sankaranarayanan
S
Meenakshisundaram
D000704:Analysis of Variance; D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D019149:Bioreactors; D056808:Biostatistics / Q000379:methods; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D008962:Models, Theoretical; D010226:Parabens / Q000378:metabolism; D012248:Rhodotorula / Q000254:growth & development / Q000378:metabolism*; D013024:Soybean Oil; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis
D010226:Parabens; D014451:Ubiquinone; D013024:Soybean Oil; C024989:coenzyme Q10; C038193:4-hydroxybenzoic acid
Rhodotorula glutinis; coenzyme Q10; para-hydroxybenzoic acid; response surface methodology; soybean oil