Development of oxacillin resistance in a patient with recurrent Staphylococcus aureus bacteremia.
Whole-genome sequencing was used to compare longitudinal isolates of Staphylococcus aureus that developed resistance to oxacillin (MIC up to 16 μg/ml). The mecA gene was absent. A novel 5-bp TATCC frameshift insertion in a gene encoding an ABC transporter similar to that of the teichoic acid translocation ATP-binding protein TagH and a 3-bp GCT nonframeshift insertion in the pdhA pyruvate dehydrogenase gene were detected in the oxacillin-resistant isolates.
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA eburd@emory.edu.
Eileen M
EM
Burd
Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Mohammad Tauqeer
MT
Alam
Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Karla D
KD
Passalacqua
Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Ameeta S
AS
Kalokhe
Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Molly E
ME
Eaton
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Sarah W
SW
Satola
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Colleen S
CS
Kraft
Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Timothy D
TD
Read
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D016368:Frameshift Mutation; D016680:Genome, Bacterial; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010068:Oxacillin / Q000494:pharmacology*; D012008:Recurrence; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification
D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D010068:Oxacillin