An ensemble of aptamers and antibodies for multivalent capture of cancer cells.
We developed an optimized ensemble of aptamers and antibodies that functions as a multivalent adhesive domain for the capture and isolation of cancer cells. When incorporated into a microfluidic device, the ensemble showed not only high capture efficiency, but also superior capture selectivity at a high shear stress (or high flow rate).
Interdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, PO Box 116250, Gainesville, Florida 32611, USA. hfan@ufl.edu.
Jinling
J
Zhang
Weian
W
Sheng
Z Hugh
ZH
Fan
D000912:Antibodies, Neoplasm / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis*; D002469:Cell Separation / Q000379:methods; D006801:Humans; D007938:Leukemia / Q000097:blood; D046210:Microfluidic Analytical Techniques; D009363:Neoplasm Proteins / Q000737:chemistry / Q000276:immunology; D009369:Neoplasms / Q000473:pathology*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D033081:Shear Strength
D000912:Antibodies, Neoplasm; D052157:Aptamers, Nucleotide; D009363:Neoplasm Proteins