Transient increase in circulating myeloid-derived suppressor cells after partial bladder outlet obstruction.
OBJECTIVE
Partial bladder outlet obstruction causes a significant increase in tissue and systemic oxidative stress markers and tissue inflammatory cytokine levels. Myeloid-derived suppressor cells, IFN-γ, IL-10 and aldosterone are believed to be associated with oxidative stress and inflammation. We investigated alterations in plasma myeloid-derived suppressor cells, IFN-γ, IL-10 and aldosterone levels in partial bladder outlet obstruction and after its reversal.


METHODS
Rats with surgically induced partial bladder outlet obstruction were divided into 4 groups of 3 each, including sham treated, 4-week obstruction, and 4 and 8-week obstruction with relief. Plasma levels of circulating myeloid-derived suppressor cells, IFN-γ, IL-10 and aldosterone were assessed by flow cytometry or enzyme-linked immunosorbent assay.


RESULTS
The circulating myeloid-derived suppressor cell level was markedly increased in the obstruction group compared to the sham treated group and it returned to normal in the 4 and 8-week obstruction with relief groups. Plasma IFN-γ, IL-10 and aldosterone were similarly increased in the obstruction group and returned to normal in the 4 and 8-week obstruction with relief groups.


CONCLUSIONS
Levels of circulating myeloid-derived suppressor cells, IFN-γ, IL-10 and aldosterone were increased in rats with partial bladder outlet obstruction but returned to normal after reversal. This suggests that an increase in these parameters may be a good predictive indicator of patients at increased risk for urinary symptoms.
Division of Urology, Department of Surgery (WYL, LYH, YPL), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Surgery (CCH), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Cardiology (TYY), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Chang Gung University of Science and Technology (WYL), Chia-Yi, Taiwan, Republic of China; Department of Medicine and Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University (WYL), Taoyuan, Taiwan, Republic of China; Department of Nutrition and Health Sciences, College of Health Science, Innovate Research Center of Medicine, Chang Jung Christian University (MLC), Tainan, Taiwan, Republic of China; Department of Medicine, Mackay Medical College (YHW), New Taipei City, Taiwan, Republic of China; Albany College of Pharmacy and Health Sciences and Stratton Veterans Affairs Medical Center, Albany (RML), New York.
Wei-Yu
WY
Lin
Division of Urology, Department of Surgery (WYL, LYH, YPL), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Surgery (CCH), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Cardiology (TYY), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Chang Gung University of Science and Technology (WYL), Chia-Yi, Taiwan, Republic of China; Department of Medicine and Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University (WYL), Taoyuan, Taiwan, Republic of China; Department of Nutrition and Health Sciences, College of Health Science, Innovate Research Center of Medicine, Chang Jung Christian University (MLC), Tainan, Taiwan, Republic of China; Department of Medicine, Mackay Medical College (YHW), New Taipei City, Taiwan, Republic of China; Albany College of Pharmacy and Health Sciences and Stratton Veterans Affairs Medical Center, Albany (RML), New York.
Ching Chuan
CC
Hsieh
Division of Urology, Department of Surgery (WYL, LYH, YPL), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Surgery (CCH), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Cardiology (TYY), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Chang Gung University of Science and Technology (WYL), Chia-Yi, Taiwan, Republic of China; Department of Medicine and Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University (WYL), Taoyuan, Taiwan, Republic of China; Department of Nutrition and Health Sciences, College of Health Science, Innovate Research Center of Medicine, Chang Jung Christian University (MLC), Tainan, Taiwan, Republic of China; Department of Medicine, Mackay Medical College (YHW), New Taipei City, Taiwan, Republic of China; Albany College of Pharmacy and Health Sciences and Stratton Veterans Affairs Medical Center, Albany (RML), New York.
Teng-Yao
TY
Yang
Division of Urology, Department of Surgery (WYL, LYH, YPL), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Surgery (CCH), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Cardiology (TYY), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Chang Gung University of Science and Technology (WYL), Chia-Yi, Taiwan, Republic of China; Department of Medicine and Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University (WYL), Taoyuan, Taiwan, Republic of China; Department of Nutrition and Health Sciences, College of Health Science, Innovate Research Center of Medicine, Chang Jung Christian University (MLC), Tainan, Taiwan, Republic of China; Department of Medicine, Mackay Medical College (YHW), New Taipei City, Taiwan, Republic of China; Albany College of Pharmacy and Health Sciences and Stratton Veterans Affairs Medical Center, Albany (RML), New York.
Miaw-Ling
ML
Chen
Division of Urology, Department of Surgery (WYL, LYH, YPL), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Surgery (CCH), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Cardiology (TYY), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Chang Gung University of Science and Technology (WYL), Chia-Yi, Taiwan, Republic of China; Department of Medicine and Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University (WYL), Taoyuan, Taiwan, Republic of China; Department of Nutrition and Health Sciences, College of Health Science, Innovate Research Center of Medicine, Chang Jung Christian University (MLC), Tainan, Taiwan, Republic of China; Department of Medicine, Mackay Medical College (YHW), New Taipei City, Taiwan, Republic of China; Albany College of Pharmacy and Health Sciences and Stratton Veterans Affairs Medical Center, Albany (RML), New York.
Li Ying
LY
Huang
Division of Urology, Department of Surgery (WYL, LYH, YPL), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Surgery (CCH), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Cardiology (TYY), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Chang Gung University of Science and Technology (WYL), Chia-Yi, Taiwan, Republic of China; Department of Medicine and Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University (WYL), Taoyuan, Taiwan, Republic of China; Department of Nutrition and Health Sciences, College of Health Science, Innovate Research Center of Medicine, Chang Jung Christian University (MLC), Tainan, Taiwan, Republic of China; Department of Medicine, Mackay Medical College (YHW), New Taipei City, Taiwan, Republic of China; Albany College of Pharmacy and Health Sciences and Stratton Veterans Affairs Medical Center, Albany (RML), New York.
Yi-Pai
YP
Lin
Division of Urology, Department of Surgery (WYL, LYH, YPL), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Surgery (CCH), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Cardiology (TYY), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Chang Gung University of Science and Technology (WYL), Chia-Yi, Taiwan, Republic of China; Department of Medicine and Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University (WYL), Taoyuan, Taiwan, Republic of China; Department of Nutrition and Health Sciences, College of Health Science, Innovate Research Center of Medicine, Chang Jung Christian University (MLC), Tainan, Taiwan, Republic of China; Department of Medicine, Mackay Medical College (YHW), New Taipei City, Taiwan, Republic of China; Albany College of Pharmacy and Health Sciences and Stratton Veterans Affairs Medical Center, Albany (RML), New York.
Pey-Jium
PJ
Chang
Division of Urology, Department of Surgery (WYL, LYH, YPL), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Surgery (CCH), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Cardiology (TYY), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Chang Gung University of Science and Technology (WYL), Chia-Yi, Taiwan, Republic of China; Department of Medicine and Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University (WYL), Taoyuan, Taiwan, Republic of China; Department of Nutrition and Health Sciences, College of Health Science, Innovate Research Center of Medicine, Chang Jung Christian University (MLC), Tainan, Taiwan, Republic of China; Department of Medicine, Mackay Medical College (YHW), New Taipei City, Taiwan, Republic of China; Albany College of Pharmacy and Health Sciences and Stratton Veterans Affairs Medical Center, Albany (RML), New York.
Robert M
RM
Levin
Division of Urology, Department of Surgery (WYL, LYH, YPL), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Surgery (CCH), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Cardiology (TYY), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Chang Gung University of Science and Technology (WYL), Chia-Yi, Taiwan, Republic of China; Department of Medicine and Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University (WYL), Taoyuan, Taiwan, Republic of China; Department of Nutrition and Health Sciences, College of Health Science, Innovate Research Center of Medicine, Chang Jung Christian University (MLC), Tainan, Taiwan, Republic of China; Department of Medicine, Mackay Medical College (YHW), New Taipei City, Taiwan, Republic of China; Albany College of Pharmacy and Health Sciences and Stratton Veterans Affairs Medical Center, Albany (RML), New York.
Yau-Huei
YH
Wei
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D015653:Cystectomy; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D008297:Male; D022423:Myeloid Cells / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D001743:Urinary Bladder / Q000378:metabolism / Q000473:pathology*; D001748:Urinary Bladder Neck Obstruction / Q000378:metabolism / Q000473:pathology* / Q000601:surgery
D015415:Biomarkers; D016207:Cytokines
cell differentiation; cytokines; inflammation; oxidative stress; urinary bladder neck obstruction