Methods, Challenges and Potentials of Single Cell RNA-seq.
RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene regulation, and how these aspects depend on each other. Here, I review the current single cell RNA-seq (scRNA-seq) efforts and discuss experimental protocols, challenges and potentials.
The University of Warwick, School of Life Sciences, Coventry CV4 7AL, UK. d.hebenstreit@warwick.ac.uk.
Daniel
D
Hebenstreit