Cytotoxic triterpenoid glycosides from the roots of Camellia oleifera.
Eight new triterpenoid saponins, oleiferosides A-H (1-8), were isolated from the EtOH extract of the roots of Camellia oleifera. Their structures were elucidated by a combination of 1D and 2D NMR techniques, mass spectrometry, and chemical methods. All were characterized to be oleanane-type saponins with sugar moieties linked to C-3 of the aglycone. Cytotoxic activities of these saponins were evaluated against four human tumor cell lines (A549, B16, BEL-7402, and MCF-7) by using the MTT in vitro assay. Compound 3 exhibited potent cytotoxic activitiy against all the tested cell lines with IC50 values < 10 µM. Compounds 1, 2, 4, and 5 showed moderate cytotoxic activities toward the tested cell lines.
College of Pharmaceutical Science, Soochow University, Suzhou, China.
Xia
X
Li
National Center for Natural Products Research, and Department of Pharmacognosy, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, Mississippi, USA.
Jianping
J
Zhao
College of Pharmaceutical Science, Soochow University, Suzhou, China.
Cuiping
C
Peng
College of Pharmaceutical Science, Soochow University, Suzhou, China.
Zhong
Z
Chen
College of Pharmaceutical Science, Soochow University, Suzhou, China.
Yanli
Y
Liu
College of Pharmaceutical Science, Soochow University, Suzhou, China.
Qiongming
Q
Xu
College of Pharmaceutical Science, Soochow University, Suzhou, China.
Ikhlas A
IA
Khan
College of Pharmaceutical Science, Soochow University, Suzhou, China.
Shilin
S
Yang
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D028244:Camellia / Q000737:chemistry*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D012503:Saponins; D014315:Triterpenes