Identification of photoperiod-regulated gene in soybean and functional analysis in Nicotiana benthamiana.
Soybean (Glycine max) is a short-day crop and the photoperiod is a crucial factor regulating its flowering time. To investigate the molecular mechanism controlling the flowering time by photoperiod in soybean, cDNA-amplified fragment length polymorphism (cDNA-AFLP) was used to identify photoperiod-regulated genes in leaves of soybean growing under short-day length, neutral photoperiod and long-day length. A total of 36 transcript-derived fragments (TDFs) were identified to be regulated by photoperiod. Among them, 26 TDFs were homologues of genes with known function. These genes are involved in secondary metabolism, cellular metabolism, cell wall components metabolism, ion transport and hormone signalling. Silencing of the homologue genes in Nicotiana benthamiana for 14 TDFs was conducted by virus-induced gene silencing. The flowering time was delayed by silencing of the genes encoding rhodanese and 40S ribosomal protein S4 (RPS4). The results indicated that rhodanese and RPS4 probably play important roles in regulating flowering time.
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, People's Republic of China. yhchen@hainu.edu.cn.
Sha
S
Ai-Hua
Chen
C
Yin-Hua
Shan
S
Zhi-Hui
Zhang
Z
Xiao-Juan
Wu
W
Xue-Jun
Qiu
Q
De-Zheng
Zhou
Z
Xin-An
D054458:Amplified Fragment Length Polymorphism Analysis; D018076:DNA, Complementary / Q000235:genetics; D035264:Flowers / Q000235:genetics / Q000821:virology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D010641:Phenotype; D017440:Photoperiod; D010788:Photosynthesis / Q000235:genetics*; D015203:Reproducibility of Results; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000821:virology
D018076:DNA, Complementary