DNA crystals as vehicles for biocatalysis.
Here we demonstrate that protein enzymes captured in the solvent channels of three-dimensional DNA crystals are catalytically active. Using RNase A as a model enzyme system, we show that crystals infused with enzyme can cleave a dinucleotide substrate with similar kinetic restrictions as other immobilized enzyme systems. This new vehicle for immobilized enzymes, created entirely from biomolecules, opens possibilities for developing modular solid-state catalysts that could be both biocompatible and biodegradable.
Department of Chemistry & Biochemistry, Center for Biomolecular Structure and Organization, and Maryland NanoCenter, University of Maryland , College Park, Maryland 20742, United States.
Chun
C
Geng
Paul J
PJ
Paukstelis
D055162:Biocatalysis; D003460:Crystallization; D004247:DNA / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004800:Enzymes, Immobilized / Q000737:chemistry; D007700:Kinetics; D009711:Nucleotides / Q000737:chemistry; D012259:Ribonuclease, Pancreatic / Q000037:antagonists & inhibitors / Q000737:chemistry
D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D009711:Nucleotides; D004247:DNA; D012259:Ribonuclease, Pancreatic