[Estimation of ATP-dependent K(+)-channel contribution to potential-dependent potassium uptake in the rat brain mitochondria].
The effect of potassium on state 4 respiration (substrate oxidation in the absence of ADP) was investigated. It was shown that potential-dependent potassium uptake in the brain mitochondria results in mitochondrial depolarization. Taking into account depolarization effect of potassium, the contribution of the endogenous proton leak as well as K(+)-uptake to the respiration rate was calculated. It was shown that such estimation allows the share of ATP-dependent potassium channel contribution to potential-dependent potassium uptake to be determined by polarographic method.
O V
OV
Akopova
V I
VI
Nosar'
L I
LI
Kolchinskaia
I N
IN
Man'kovskaia
M K
MK
Malysheva
V F
VF
Sagach
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects; D054086:KATP Channels / Q000378:metabolism*; D007700:Kinetics; D008314:Malonates / Q000378:metabolism* / Q000494:pharmacology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D011188:Potassium / Q000378:metabolism* / Q000494:pharmacology; D011522:Protons; D051381:Rats; D017208:Rats, Wistar
D054086:KATP Channels; D008314:Malonates; D011522:Protons; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C030290:malonic acid; D011188:Potassium