Nuclear and mitochondrial patterns of population structure in North Pacific false killer whales (Pseudorca crassidens).
False killer whales (Pseudorca crassidens) are large delphinids typically found in deep water far offshore. However, in the Hawaiian Archipelago, there are 2 resident island-associated populations of false killer whales, one in the waters around the main Hawaiian Islands (MHI) and one in the waters around the Northwestern Hawaiian Islands (NWHI). We use mitochondrial DNA (mtDNA) control region sequences and genotypes from 16 nuclear DNA (nucDNA) microsatellite loci from 206 individuals to examine levels of differentiation among the 2 island-associated populations and offshore animals from the central and eastern North Pacific. Both mtDNA and nucDNA exhibit highly significant differentiation between populations, confirming limited gene flow in both sexes. The mtDNA haplotypes exhibit a strong pattern of phylogeographic concordance, with island-associated populations sharing 3 closely related haplotypes not found elsewhere in the Pacific. However, nucDNA data suggest that NWHI animals are at least as differentiated from MHI animals as they are from offshore animals. The patterns of differentiation revealed by the 2 marker types suggest that the island-associated false killer whale populations likely share a common colonization history, but have limited contemporary gene flow.
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz) Karen.Martien@noaa.gov.
Karen K
KK
Martien
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Susan J
SJ
Chivers
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Robin W
RW
Baird
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Frederick I
FI
Archer
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Antoinette M
AM
Gorgone
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Brittany L
BL
Hancock-Hanser
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
David
D
Mattila
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Daniel J
DJ
McSweeney
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Erin M
EM
Oleson
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Carol
C
Palmer
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Victoria L
VL
Pease
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Kelly M
KM
Robertson
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Gregory S
GS
Schorr
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Mark B
MB
Schultz
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Daniel L
DL
Webster
From the NOAA Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA 92037 (Martien, Chivers, Archer, Hancock-Hanser, Pease, Robertson, and Taylor); Cascadia Research Collective 218 1/2 W, 4th Avenue, Olympia, WA 98501 (Baird, Schorr, and Webster); NOAA Southeast Fisheries Science Center, 101 Pivers Island Road, Beaufort, NC 28516 (Gorgone); Hawaiian Islands Humpback Whale National Marine Sanctuary, 726 S. Kihei Road, Kihei, HI 96753 (Mattila); Wild Whale Research Foundation, Box 139, Holualoa, HI 96725 (McSweeney); NOAA Pacific Islands Fisheries Science Center, 2470 Dole St., Honolulu, HI 96822 (Oleson); Department of Land Resource Management, PO Box 496, Palmerston, NT 0831, Australia (Palmer); Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina NT 0811, Australia (Palmer); and the University of Melbourne, Faculty of Medical and Dental Health Sciences, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, Parkville, Victoria 3010, Australia (Schultz).
Barbara L
BL
Taylor
D000483:Alleles; D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D004289:Dolphins / Q000235:genetics*; D051456:Gene Flow; D056426:Genetic Loci; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D006254:Hawaii; D018895:Microsatellite Repeats / Q000235:genetics; D005810:Multigene Family; D058974:Phylogeography; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial
cetacean; island-associated; male-mediated gene flow; population structure; social structure