Short noncoding DNA fragments improve the immune potency of electroporation-mediated HBV DNA vaccination.
Electroporation (EP)-mediated DNA immunization can elicit effective immune responses in a variety of animals, and is widely used in research studies and clinical trials. However, high-pulse voltage, high DNA dose and multiple immunizations are still required to achieve considerable immune responses. To further improve the efficiency of EP-mediated DNA immunization, many parameters have been tried and optimized in recent years. In our early research, we found that the short noncoding DNA fragments (sf-DNA) can significantly enhance EP-mediated transgene expression of reporter genes. In this study, we tested the effect of sf-DNA on the immune potency of EP-mediated hepatitis B virus (HBV) DNA vaccination in a mouse model. The results show that the use of sf-DNA in EP-mediated HBV DNA vaccination leads to an enhanced expression of the HBV surface antigen, resulting in higher cellular and humoral responses. Furthermore, the immune responses in the sf-DNA-mediated 120 V cm(-1) EP immunization group were higher than that of the 200 V cm(-1) EP without sf-DNA groups. These data suggest that the sf-DNA can be used as an effective helper molecule to improve the immune response of EP-mediated HBV DNA vaccination, which may make the EP-mediated DNA vaccination more effective and suitable for animal and clinical application.
School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
J
J
Peng
School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
S
S
Shi
School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
Z
Z
Yang
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
Q
Q
Ding
School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
W
W
Hai
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
H
H
Tang
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
Y
Y
Yang
Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, CA, USA.
J R
JR
Bernstein
Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, CA, USA.
P
P
Peyda
1] School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China [2] School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
Y
Y
Xu
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D018274:Electroporation / Q000379:methods*; D005260:Female; D005822:Genetic Vectors / Q000008:administration & dosage; D006509:Hepatitis B / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000378:metabolism; D017325:Hepatitis B Vaccines / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D018482:Muscle, Skeletal / Q000276:immunology / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000276:immunology*
D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D019444:Vaccines, DNA; D000469:Alkaline Phosphatase