Comparison of Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR in predominantly adult hospitalized patients.
To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-antibody assay (DFA) detected 132 (66.3%) positive samples, Simplexa direct detected 162 (81.4%), Simplexa using extracts detected 177 (88.9%), and lab-developed TaqMan PCR reference methods detected 199 (100%). The specificities were 99.6%, 100%, 100%, and 100%, respectively. The two Simplexa methods were more sensitive than the DFA (P = 0.0001) but less sensitive than the TaqMan reverse transcriptase PCR (P = 0.0001).
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut, USA Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, USA Clinical Virology Laboratory, Yale-New Haven Hospital, New Haven, Connecticut, USA marie.landry@yale.edu.
Marie L
ML
Landry
Clinical Virology Laboratory, Yale-New Haven Hospital, New Haven, Connecticut, USA.
David
D
Ferguson
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods; D006761:Hospitals; D006801:Humans; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D008875:Middle Aged; D009305:Nasopharynx / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity