Chemical composition and antimicrobial activity of the essential oil of Juniperus excelsa M.Bieb. growing wild in Lebanon.
The essential oils (EOs) isolated from the leaves and twigs of Juniperus excelsa M.Bieb. growing wild in Lebanon were characterized, and their antimicrobial activity and antiradical capacity were evaluated. The EOs were obtained by hydrodistillation using a Clevenger-type apparatus and characterized by GC and GC/MS analyses. The antimicrobial activity was evaluated by determining minimal inhibitory concentrations (MICs) against a Gram-positive and a Gram-negative bacterium, a yeast, and a dermatophyte with the broth microdilution technique. A total of 28 constituents was identified and accounted for 90.1 and 95.6% of the twig and leaf EO composition, respectively. Both EOs were essentially composed of monoterpene hydrocarbons (46.7 and 59.6% for twig and leaf EOs, resp.) and sesquiterpenes (39.4 and 32.1%, resp.). The main components were α-pinene, α-cedrol, and δ-car-3-ene. The J. excelsa EOs did not show any antiradical potential, but revealed interesting in vitro antimicrobial activities against Staphylococcus aureus and Trichophyton rubrum (MICs of 64 and 128 μg/ml, resp.). The three major compounds were tested separately and in combination according to their respective amounts in the oil. δ-Car-3-ene was the most active component and is undoubtedly one of the constituents driving the antifungal activity of J. excelsa essential oil, even though synergies are probably involved.
CNRS, Institut de Chimie des Substances Naturelles, UPR 2301, 1 Avenue de la Terrasse, F-91198, Gif-sur-Yvette (phone: +33-1-69823610; fax: +33-1-69823784); Department of Agricultural Sciences, Holy Spirit University of Kaslik, Kaslik, B.P. 446, Jounieh, Lebanon.
Madona
M
Khoury
Marc
M
El Beyrouthy
Naïm
N
Ouaini
Marcello
M
Iriti
Véronique
V
Eparvier
Didier
D
Stien
D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D005658:Fungi / Q000187:drug effects; D006801:Humans; D018653:Juniperus / Q000737:chemistry*; D009181:Mycoses / Q000188:drug therapy; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
D000890:Anti-Infective Agents; D009822:Oils, Volatile
Antimicrobial activity; Antioxidant activity; Essential oils; Juniperus excelsa; α-Cedrol; α-Pinene; δ-Car-3-ene