Eicosapentaenoic and docosahexaenoic acid ethyl esters differentially enhance B-cell activity in murine obesity.
EPA and DHA are not biologically equivalent; however, their individual activity on B cells is unknown. We previously reported fish oil enhanced murine B-cell activity in obesity. To distinguish between the effects of EPA and DHA, we studied the ethyl esters of EPA and DHA on murine B-cell function as a function of time. We first demonstrate that EPA and DHA maintained the obese phenotype, with no improvements in fat mass, adipose inflammatory cytokines, fasting insulin, or glucose clearance. We then tested the hypothesis that EPA and DHA would increase the frequency of splenic B cells. EPA and DHA differentially enhanced the frequency and/or percentage of select B-cell subsets, correlating with increased natural serum IgM and cecal IgA. We next determined the activities of EPA and DHA on ex vivo production of cytokines upon lipopolysaccharide stimulation of B cells. EPA and DHA, in a time-dependent manner, enhanced B-cell cytokines with DHA notably increasing IL-10. At the molecular level, EPA and DHA differentially enhanced the formation of ordered microdomains but had no effect on Toll-like receptor 4 mobility. Overall, the results establish differential effects of EPA and DHA in a time-dependent manner on B-cell activity in obesity, which has implications for future clinical studies.
Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC.
Heather
H
Teague
Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC.
Mitchel
M
Harris
Department of Food Science and Nutrition, Michigan State University, East Lansing, MI.
Jenifer
J
Fenton
Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC.
Perrine
P
Lallemand
Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC.
Brian M
BM
Shewchuk
Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC.
Saame Raza
SR
Shaikh
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004537:Eicosanoic Acids / Q000494:pharmacology*; D007070:Immunoglobulin A / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D008297:Male; D051379:Mice; D009765:Obesity / Q000276:immunology* / Q000473:pathology
D004537:Eicosanoic Acids; C556062:IL10 protein, mouse; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D016753:Interleukin-10; D004281:Docosahexaenoic Acids
high fat diets; immunity; metabolism; n-3 polyunsaturated fatty acids