An immortalized human liver endothelial sinusoidal cell line for the study of the pathobiology of the liver endothelium.
The endothelium lines blood and lymph vessels and protects underlying tissues against external agents such as viruses, bacteria and parasites. Yet, microbes and particularly viruses have developed sophisticated ways to bypass the endothelium in order to gain access to inner organs. De novo infection of the liver parenchyma by many viruses and notably hepatitis viruses, is thought to occur through recruitment of virions on the sinusoidal endothelial surface and subsequent transfer to the epithelium. Furthermore, the liver endothelium undergoes profound changes with age and in inflammation or infection. However, primary human liver sinusoidal endothelial cells (LSECs) are difficult to obtain due to scarcity of liver resections. Relevant derived cell lines are needed in order to analyze in a standardized fashion the transfer of pathogens across the liver endothelium. By lentiviral transduction with hTERT only, we have immortalized human LSECs isolated from a hereditary hemorrhagic telangiectasia (HHT) patient and established the non-transformed cell line TRP3. TRP3 express mesenchymal, endothelial and liver sinusoidal markers. Functional assessment of TRP3 cells demonstrated a high capacity of endocytosis, tube formation and reactivity to immune stimulation. However, TRP3 displayed few fenestrae and expressed C-type lectins intracellularly. All these findings were confirmed in the original primary LSECs from which TRP3 were derived suggesting that these features were already present in the liver donor. We consider TRP3 as a model to investigate the functionality of the liver endothelium in hepatic inflammation in infection.
INSERM U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France; Université de Lyon, F-69000 Lyon, France; DevWeCan Laboratories of Excellence Network (Labex), France.
Romain
R
Parent
INSERM U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France; Université de Lyon, F-69000 Lyon, France; DevWeCan Laboratories of Excellence Network (Labex), France.
David
D
Durantel
INSERM U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France; Université de Lyon, F-69000 Lyon, France.
Thomas
T
Lahlali
INSERM U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France; Université de Lyon, F-69000 Lyon, France.
Aurélie
A
Sallé
INSERM U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France; Université de Lyon, F-69000 Lyon, France.
Marie-Laure
ML
Plissonnier
INSERM U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France; Université de Lyon, F-69000 Lyon, France.
Daniel
D
DaCosta
INSERM U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France; Université de Lyon, F-69000 Lyon, France; Service de Genetique Moleculaire et Clinique, CHRU Lyon, Hopital Edouard Herriot, Lyon, France.
Gaëtan
G
Lesca
INSERM U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France; Université de Lyon, F-69000 Lyon, France; DevWeCan Laboratories of Excellence Network (Labex), France; Hospices Civils de Lyon (HCL), Lyon, France.
Fabien
F
Zoulim
INSERM U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France; Université de Lyon, F-69000 Lyon, France.
Marie-Jeanne
MJ
Marion
INSERM U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France; Université de Lyon, F-69000 Lyon, France; DevWeCan Laboratories of Excellence Network (Labex), France. Electronic address: Birke.Bartosch@inserm.fr.
Birke
B
Bartosch
D000368:Aged; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D002470:Cell Survival; D042783:Endothelial Cells / Q000145:classification / Q000166:cytology* / Q000502:physiology*; D005260:Female; D022781:Hepatocytes / Q000145:classification / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000502:physiology*
Adhesion molecules; Differentiation; Inflammation; Liver sinusoidal endothelial cells; Tubulogenesis; Uptake