Activity exerted by a testosterone derivative on myocardial injury using an ischemia/reperfusion model.
Some reports indicate that several steroid derivatives have activity at cardiovascular level; nevertheless, there is scarce information about the activity exerted by the testosterone derivatives on cardiac injury caused by ischemia/reperfusion (I/R). Analyzing these data, in this study, a new testosterone derivative was synthetized with the objective of evaluating its effect on myocardial injury using an ischemia/reperfusion model. In addition, perfusion pressure and coronary resistance were evaluated in isolated rat hearts using the Langendorff technique. Additionally, molecular mechanism involved in the activity exerted by the testosterone derivative on perfusion pressure and coronary resistance was evaluated by measuring left ventricular pressure in the absence or presence of the following compounds: flutamide, prazosin, metoprolol, nifedipine, indomethacin, and PINANE TXA2. The results showed that the testosterone derivative significantly increases (P = 0.05) the perfusion pressure and coronary resistance in isolated heart. Other data indicate that the testosterone derivative increases left ventricular pressure in a dose-dependent manner (0.001-100 nM); however, this phenomenon was significantly inhibited (P = 0.06) by indomethacin and PINANE-TXA2  (P = 0.05) at a dose of 1 nM. In conclusion, these data suggest that testosterone derivative induces changes in the left ventricular pressure levels through thromboxane receptor activation.
Laboratory of Pharmaco-Chemistry, Faculty of Chemical Biological Sciences, University Autonomous of Campeche, Avenida Agustín Melgar s/n, Colonia Buenavista, 24039 San Francisco de Campeche, CAM, Mexico.
Figueroa-Valverde
FV
Lauro
Escuela Nacional de Ciencias Biológicas del Instituto Politéecnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomas, 11340 Mexico City, DF, Mexico.
Díaz-Cedillo
DC
Francisco
Laboratory of Pharmaco-Chemistry, Faculty of Chemical Biological Sciences, University Autonomous of Campeche, Avenida Agustín Melgar s/n, Colonia Buenavista, 24039 San Francisco de Campeche, CAM, Mexico.
García-Cervera
GC
Elodia
Laboratory of Pharmaco-Chemistry, Faculty of Chemical Biological Sciences, University Autonomous of Campeche, Avenida Agustín Melgar s/n, Colonia Buenavista, 24039 San Francisco de Campeche, CAM, Mexico.
Pool-Gómez
PG
Eduardo
Laboratory of Pharmaco-Chemistry, Faculty of Chemical Biological Sciences, University Autonomous of Campeche, Avenida Agustín Melgar s/n, Colonia Buenavista, 24039 San Francisco de Campeche, CAM, Mexico.
López-Ramos
LR
Maria
Facultad de Nutrición, Médicos y Odontologos s/n, Unidad del Bosque, 91010 Xalapa, VER, Mexico.
Rosas-Nexticapa
RN
Marcela
Laboratory of Pharmaco-Chemistry, Faculty of Chemical Biological Sciences, University Autonomous of Campeche, Avenida Agustín Melgar s/n, Colonia Buenavista, 24039 San Francisco de Campeche, CAM, Mexico.
Hau-Heredia
HH
Lenin
Faculty of Medicine, University Autonomous of Campeche, Avenida Patricio Trueba de Regil s/n, Col Lindavista, 24090 San Francisco de Campeche, CAM, Mexico.
Sarabia-Alcocer
SA
Betty
Faculty of Medicine, University Autonomous of Campeche, Avenida Patricio Trueba de Regil s/n, Col Lindavista, 24090 San Francisco de Campeche, CAM, Mexico.
Velázquez-Sarabia Betty
VS
Monica
D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects*; D003331:Coronary Vessels / Q000503:physiopathology*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000031:analogs & derivatives / Q000494:pharmacology; D014655:Vascular Resistance / Q000187:drug effects*
D013739:Testosterone