Adrenal CYP11B1/2 expression in primary aldosteronism: immunohistochemical analysis using novel monoclonal antibodies.
CYP11B1 and CYP11B2 play pivotal roles in adrenocorticosteroids synthesis. We performed semi-quantitative immunohistochemical analysis of these proteins in adrenals from patients with primary aldosteronism using novel monoclonal antibodies. Clusters of cortical cells positive for CYP11B2 were detected in the zona glomerulosa (ZG) of normal adrenal gland (NA), idiopathic hyperaldosteronism (IHA) and the adjacent adrenal of aldosterone-producing adenoma (APA). In APA, heterogenous immunolocalization of CYP11B2 and diffuse immunoreactivity of CYP11B1 were detected in tumor cells, respectively. The relative immunoreactivity of CYP11B2 in the ZG of adjacent adrenal of APA was significantly lower than that of NA, IHA and APA tumor cells, suggestive of suppressed aldosterone biosynthesis in these cells. These findings did indicate the regulatory mechanisms of aldosterone biosynthesis were different between normal/hyperplastic and neoplastic aldosterone-producing cells in human adrenals. CYP11B2 immunoreactivity in the ZG could also serve as a potential immunohistochemical marker differentiating morphologically hyperplastic ZG of IHA and APA adjacent adrenal.
Tohoku University Graduate School of Medicine, Department of Pathology, Sendai, Japan.
Yasuhiro
Y
Nakamura
Tohoku University Graduate School of Medicine, Department of Pathology, Sendai, Japan.
Takashi
T
Maekawa
Tohoku University Graduate School of Medicine, Department of Pathology, Sendai, Japan.
Saulo J A
SJ
Felizola
Tohoku University Hospital, Division of Nephrology and Hypertension, Sendai, Japan.
Fumitoshi
F
Satoh
Endocrinology, University of Mississippi Medical Center, MS, USA.
Xin
X
Qi
Endocrinology, University of Mississippi Medical Center, MS, USA.
Carolina
C
Velarde-Miranda
Endocrinology, University of Mississippi Medical Center, MS, USA.
Maria W
MW
Plonczynski
Tohoku University Graduate School of Medicine, Department of Pathology, Sendai, Japan.
Kazue
K
Ise
Tohoku University Hospital, Division of Nephrology and Hypertension, Sendai, Japan.
Kumi
K
Kikuchi
Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
William E
WE
Rainey
Endocrine Section, G.V. (Sonny) Montgomery VA Medical Center, AL, USA; Endocrinology, University of Mississippi Medical Center, MS, USA; Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, MS, USA.
Elise P
EP
Gomez-Sanchez
Endocrine Section, G.V. (Sonny) Montgomery VA Medical Center, AL, USA; Endocrinology, University of Mississippi Medical Center, MS, USA.
Celso E
CE
Gomez-Sanchez
Tohoku University Graduate School of Medicine, Department of Pathology, Sendai, Japan. Electronic address: hsasano@patholo2.med.tohoku.ac.jp.
Hironobu
H
Sasano
D000302:Adrenal Cortex / Q000201:enzymology / Q000473:pathology; D000311:Adrenal Glands / Q000201:enzymology* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D019405:Cytochrome P-450 CYP11B2 / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006929:Hyperaldosteronism / Q000201:enzymology*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011375:Progesterone Reductase / Q000378:metabolism; D021381:Protein Transport; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism*; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism
D000911:Antibodies, Monoclonal; D011375:Progesterone Reductase; D013254:Steroid 17-alpha-Hydroxylase; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase
APA; Adrenal cortex; CYP11B1; CYP11B2; IHA; Monoclonal antibody