Bacterial colonization of a fumigated alkaline saline soil.
After chloroform fumigating an arable soil, the relative abundance of phylotypes belonging to only two phyla (Actinobacteria and Firmicutes) and two orders [Actinomycetales and Bacillales (mostly Bacillus)] increased in a subsequent aerobic incubation, while it decreased for a wide range of bacterial groups. It remained to be seen if similar bacterial groups were affected when an extreme alkaline saline soil was fumigated. Soil with electrolytic conductivity between 139 and 157 dS m(-1), and pH 10.0 and 10.3 was fumigated and the bacterial community structure determined after 0, 1, 5 and 10 days by analysis of the 16S rRNA gene, while an unfumigated soil served as control. The relative abundance of the Firmicutes increased in the fumigated soil (52.8%) compared to the unfumigated soil (34.2%), while that of the Bacteroidetes decreased from 16.2% in the unfumigated soil to 8.8% in the fumigated soil. Fumigation increased the relative abundance of the genus Bacillus from 14.7% in the unfumigated soil to 25.7%. It was found that phylotypes belonging to the Firmicutes, mostly of the genus Bacillus, were dominant in colonizing the fumigated alkaline saline as found in the arable soil, while the relative abundance of a wide range of bacterial groups decreased.
Laboratory of Soil Ecology, ABACUS, Cinvestav, Avenida Instituto Politécnico Nacional 2508, 07360, Mexico D.F., Mexico.
Juan M
JM
Bello-López
Cristina A
CA
Domínguez-Mendoza
Arit S
AS
de León-Lorenzana
Laura
L
Delgado-Balbuena
Yendi E
YE
Navarro-Noya
Selene
S
Gómez-Acata
Analine
A
Rodríguez-Valentín
Victor M
VM
Ruíz-Valdiviezo
Marco
M
Luna-Guido
Nele
N
Verhulst
Bram
B
Govaerts
Luc
L
Dendooven
D000192:Actinomycetales / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D000468:Alkalies / Q000494:pharmacology; D001407:Bacillus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D002725:Chloroform / Q000494:pharmacology; D005651:Fumigation; D064307:Microbiota; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D000468:Alkalies; D012336:RNA, Ribosomal, 16S; D002725:Chloroform