Anti-inflammatory and antioxidant activities, functional properties and mutagenicity studies of protein and protein hydrolysate obtained from Prosopis alba seed flour.
Prosopis species are considered multipurpose trees and shrubs by FAO and their fruit constitute a food source for humans and animals. According to the "Código Alimentario Argentino", "algarrobo flour" is produced by grinding the whole mature pod, but in the traditional process most of the seeds are discarded. In this paper, the flour from seed was obtained. Then, the proteins were extracted and enzymatic hydrolysis was carried out. According to their amino acid profile and chemical score (>100%), the Prosopis alba proteins, are not deficient in essential amino acids considering the amount of amino acid necessary by adults. The protein isolate showed a good solubility (pH 7.4-9), emulsificant capacity, oil binding capacity and water adsorption capacity. The antioxidant ability of proteins was significantly increased with hydrolysis (SC50 values: 50-5μg/mL, respectively). Inhibitory activity of pro-inflammatory enzymes (lipoxygenase and phospholipase) was described. The mutagenicity/antimutagenicity of proteins and protein hydrolysates from seed flour were also analysed. The results suggest that P. alba cotyledon flour could be a new alternative in the formulation of functional foods not only for its high protein content but also by the biological and functional properties of its proteins and protein hydrolysates.
INQUINOA (CONICET), Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina.
Florencia
F
Cattaneo
INQUINOA (CONICET), Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina; Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina; Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina.
Jorge Esteban
JE
Sayago
INQUINOA (CONICET), Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina; Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina; Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina.
María Rosa
MR
Alberto
INQUINOA (CONICET), Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina; Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina; Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina.
Iris Catiana
IC
Zampini
INQUINOA (CONICET), Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina; Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina; Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina.
Roxana Mabel
RM
Ordoñez
Instituto Tecnología de Alimentos, INTA, Buenos Aires, Argentina.
Verónica
V
Chamorro
Instituto Tecnología de Alimentos, INTA, Buenos Aires, Argentina.
Adriana
A
Pazos
INQUINOA (CONICET), Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina; Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina; Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, 4000 S.M. de Tucumán, Tucumán, Argentina. Electronic address: misla@tucbbs.com.ar.
María Inés
MI
Isla
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D018548:Cotyledon / Q000737:chemistry; D005433:Flour / Q000032:analysis; D006801:Humans; D009153:Mutagens / Q000633:toxicity*; D029862:Prosopis / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000494:pharmacology* / Q000633:toxicity; D012639:Seeds / Q000009:adverse effects* / Q000737:chemistry
D000596:Amino Acids; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D009153:Mutagens; D011492:Protein Hydrolysates
Antioxidant capacity; Flour; Functional properties; Lipoxygenase; Phospholipase A(2); Prosopis; Proteins; Seed