Anxiety disorders and anxiety-related traits and serotonin transporter gene-linked polymorphic region (5-HTTLPR) in adolescents: case-control and trio studies.
The role of the serotonin transporter gene-linked polymorphic region (5-HTTLPR) in anxiety disorder and anxiety-related traits is controversial. Besides this study, few studies have evaluated the triallelic genotype in adolescents. The aim of this study was to investigate whether anxiety disorders and anxiety-related traits are associated with 5-HTTLPR (biallelic and triallelic) in adolescents, integrating both case-control-based and family-based designs in a community sample. This is a cross-sectional community study of 504 individuals and their families: 225 adolescents (129 adolescents with anxiety disorder and 96 controls) and their biological families. We assessed psychiatric diagnosis using the Kiddie Schedule for Affective Disorders and Schizophrenia. The Temperament and Character Inventory and the Resnick Behavioral Inhibition Scale were used to evaluate harm avoidance and behavioral inhibition. DNA was extracted from saliva and genotyped, including biallelic and triallelic 5-HTTLPR classification, by PCR-RFLP followed by agarose gel electrophoresis. We were not able to find any associations between 5-HTTLPR and anxiety-related phenotypes in both case-control and trio analyses. Further investigation and meta-analytic studies are needed to better clarify the inconsistent results with regard to the association between 5-HTTLPR and anxiety-related phenotypes in adolescents.
aAnxiety Disorders Outpatient Program for Children and Adolescents, PROTAIA bBasic Research and Advanced Investigations in Neurosciences, BRAIN Laboratory, Hospital de Clínicas de Porto Alegre cPostgraduate Program in Neuroscience, Institute of Basic Sciences/Health, Federal University of Rio Grande do Sul, UFRGS, Porto Alegre dNational Institute of Developmental Psychiatry for Children and Adolescents, INPD, Brazilian National Council of Research, CNPq, Porto Alegre, Brazil eHealth Sciences Federal University of Porto Alegre, UFCSPA, Porto Alegre, Brazil fDepartment of Psychiatry, University of São Paulo, Medical School, São Paulo, Brazil.
Andressa
A
Bortoluzzi
Carolina
C
Blaya
Giovanni A
GA
Salum
Carolina
C
Cappi
Sandra
S
Leistner-Segal
Gisele G
GG
Manfro
D000293:Adolescent; D000483:Alleles; D001007:Anxiety / Q000235:genetics*; D001008:Anxiety Disorders / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D005190:Family; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D019655:Quantitative Trait, Heritable; D050486:Serotonin Plasma Membrane Transport Proteins / Q000235:genetics*
C493230:SLC6A4 protein, human; D050486:Serotonin Plasma Membrane Transport Proteins