Bioorganic research of Galactites tomentosa Moench. Honey extracts: enantiomeric purity of chiral marker 3-phenyllactic acid.
Thistle (Galactites tomentosa Moench.) honey organic extracts were obtained by headspace solid-phase microextraction (HS-SPME) and ultrasonic solvent extraction (USE) and analyzed by gas chromatography (GC-FID and GC-MS) for the first time. Most abundant headspace compounds were terpenes, particularly linalool derivatives (hotrienol was predominant with a range of 38.6-57.5%). 3-Phenyllactic acid dominated in the solvent extracts (77.4-86.4%) followed by minor percentages of other shikimate pathway derivatives. After determination of an adequate enantioseparation protocol on Chirallica PST-4 column, the honey solvent extracts were analyzed by high-performance liquid chromatography (HPLC). The chiral analysis revealed high enantiomeric excess (>95%) of (-)-3-phenyllactic acid in all samples. Therefore, previous findings of chemical markers of thistle honey were extended, providing new potential for advanced chemical fingerprinting (optical pure chemical marker).
Department of Organic Chemistry, Faculty of Chemistry & Technology, University of Split, Split, Croatia.
Igor
I
Jerković
Marin
M
Roje
Carlo I G
CI
Tuberoso
Zvonimir
Z
Marijanović
Ana
A
Kasum
Marina
M
Obradović
D019659:Asteraceae / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006722:Honey; D007773:Lactates / Q000737:chemistry* / Q000302:isolation & purification*; D052617:Solid Phase Microextraction; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D014465:Ultrasonics; D014835:Volatilization
D007773:Lactates; D012997:Solvents; C017648:3-phenyllactic acid
(-)-3-phenyllactic acid; GC-FID; GC-MS; chiral HPLC with polarimetric detector; volatile organic compounds