GC/MS-based metabolomics approach to identify biomarkers differentiating survivals from death in crucian carps infected by Edwardsiella tarda.
Microbial disease problems constitute the largest single cause of economic losses in aquaculture. An understanding of immune system in aquaculture animals how to function in defense against bacterial infections is especially important to control these diseases and improve food quality and safety. In the present study, we use a crucian carp model to explore which pathways and metabolites are crucial for the defense against infection caused by Edwardsiella tarda EIB202. We establish the metabolic profile of crucian carps and then compare the metabolic difference between survivals and dead fish by self-control. We identify elevating unsaturated fatty acid biosynthesis and decreasing fructose and mannose metabolism as the most key pathways and increasing palmitic acid and decreasing d-mannose as the most crucial metabolites differentiating survivals from death in these fish infected by E. tarda. Our findings highlight the importance of metabolic strategy against bacterial infections.
Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Laboratory of Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
Chang
C
Guo
Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Laboratory of Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
Xiao-Yan
XY
Huang
Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Laboratory of Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China; Tibet Vocational Technical College, Lhasha 850000, People's Republic of China.
Man-Jun
MJ
Yang
Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Laboratory of Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
Sheng
S
Wang
Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Laboratory of Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
Shi-Tong
ST
Ren
Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Laboratory of Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
Hui
H
Li
Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Laboratory of Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China. Electronic address: pxuanx@sysu.edu.cn.
Xuan-Xian
XX
Peng
D000818:Animals; D017756:Aquaculture / Q000379:methods*; D015415:Biomarkers / Q000378:metabolism*; D002347:Carps; D019295:Computational Biology; D020609:Edwardsiella tarda; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000662:veterinary*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology*; D005632:Fructose / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000662:veterinary; D008358:Mannose / Q000378:metabolism; D055432:Metabolomics / Q000379:methods; D019308:Palmitic Acid / Q000378:metabolism
D015415:Biomarkers; D005231:Fatty Acids, Unsaturated; D019308:Palmitic Acid; D005632:Fructose; D008358:Mannose
Biomarker; Crucian carp; Metabolomics; Palmitic acid; d-mannose