Probability of antibody formation against circumsporozoite protein of Plasmodium vivax among Korean malaria patients.
To evaluate the seroprevalence against circumsporozoite protein (CSP) of Plasmodium vivax in sera of Korean patients, the central repeating domain (CRD) of CSP was cloned and analyzed. From the genomic DNA of patient's blood, 2 kinds of CSPs were identified to belong to a VK210 type, which is the dominant repeating of GDRA(D/A)GQPA, and named as PvCSPA and PvCSPB. Recombinantly expressed his-tagged PvCSPA or PvCSPB in Escherichia coli reacted well against sera of patients in western blot, with the detecting rate of 47.9% (58/121), which included 15 cases positive for PvCSPA, 6 cases positive for PvCSPB, and 37 cases for both. The mixture of PvCSPA and PvCSPB was loaded to a rapid diagnostic test kit (RDT) and applied with the same set of patient sera, which resulted in detection rates of 57.0% (69/121). When the protein sequences of PvCSPA were compared with those of P. vivax in endemic regions of India and Uganda, they were compatibly homologous to PvCSPA with minor mutations. These results suggested that the recombinant PvCSPA and PvCSPB loaded RDT may be a milestone in latent diagnosis which has been a hot issue of domestic malaria and important for radical therapy in overlapped infections with P. falciparum in tropical and subtropical areas. During the biological process of malarial infection, exposure of CSP to antigen-antibody reaction up to 57.0% is the first report in Korea.
Department of Parasitology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
Ho-Woo
HW
Nam
Department of Parasitology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
Kyoung Ju
KJ
Song
Department of Parasitology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
Hye Jin
HJ
Ahn
Department of Parasitology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
Zhaoshou
Z
Yang
GenBody Inc., Cheonan 330-714, Korea.
Chom-Kyu
CK
Chong
Department of Tropical Medicine and Parasitology, Inha University School of Medicine, Incheon 400-712, Korea.
Pyo Yun
PY
Cho
Department of Tropical Medicine and Parasitology, Inha University School of Medicine, Incheon 400-712, Korea.
Seong Kyu
SK
Ahn
Department of Tropical Medicine and Parasitology, Inha University School of Medicine, Incheon 400-712, Korea.
Tong-Soo
TS
Kim
D000595:Amino Acid Sequence; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D000917:Antibody Formation; D000953:Antigens, Protozoan / Q000276:immunology; D001483:Base Sequence; D006801:Humans; D007194:India; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000453:epidemiology* / Q000276:immunology; D020066:Merozoite Surface Protein 1 / Q000235:genetics / Q000276:immunology*; D010966:Plasmodium vivax / Q000235:genetics / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000276:immunology; D056910:Republic of Korea / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D016036:Seroepidemiologic Studies; D014454:Uganda
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; C041595:circumsporozoite protein, Protozoan
Plasmodium vivax; central repeating domain; circumsporozoite protein; rapid diagnostic test; variant VK210