Enhanced antitumor immunity is elicited by adenovirus-mediated gene transfer of CCL21 and IL-15 in murine colon carcinomas.
The chemokine CCL21 is a potent chemoattractant for T cells and dendritic cells. IL-15 elicits powerful antitumor immune responses through the stimulation of natural killer cells. We constructed a CCL21/IL-15-expressing adenovirus (Ad-CCL21-IL-15) and evaluated its antitumor effects in vitro and in vivo. We found that the intratumoral injection of Ad-CCL21-IL-15 into murine colon carcinomas significantly inhibited tumor growth. Splenocytes from mice treated with Ad-CCL21-IL-15 developed tumor-specific cytotoxic T cells and were protected from subsequent challenges with tumor cells. This study indicates that providing cancer therapy by combining CCL21 and IL-15 can induce antitumor immune responses and is an effective strategy for cancer immunotherapy.
Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, China-Japan Union Hospital, Jilin University, Changchun 130062, PR China.
Dong-xu
DX
Zhao
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
Zhi-jie
ZJ
Li
First Hospital of Jilin University, Changchun, Jilin Province 130021, PR China.
Yang
Y
Zhang
First Hospital of Jilin University, Changchun, Jilin Province 130021, PR China.
Xiao-na
XN
Zhang
Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, China-Japan Union Hospital, Jilin University, Changchun 130062, PR China.
Kun-chi
KC
Zhao
Fourth Hospital of Jilin University, Changchun 130062, PR China.
Ya-gang
YG
Li
Fourth Hospital of Jilin University, Changchun 130062, PR China.
Meng-meng
MM
Zhang
First Hospital of Jilin University, Changchun, Jilin Province 130021, PR China.
Xiao-wei
XW
Yu
Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, China-Japan Union Hospital, Jilin University, Changchun 130062, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China.
Ming-yuan
MY
Liu
Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, China-Japan Union Hospital, Jilin University, Changchun 130062, PR China. Electronic address: myth0317@yeah.net.
Yang
Y
Li
D016475:3T3 Cells; D000256:Adenoviridae; D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D054421:Chemokine CCL21 / Q000235:genetics*; D003110:Colonic Neoplasms / Q000628:therapy*; D003713:Dendritic Cells / Q000276:immunology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D007167:Immunotherapy; D019409:Interleukin-15 / Q000235:genetics*; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D019496:Cancer Vaccines; D054421:Chemokine CCL21; D019409:Interleukin-15
Bicistronic adenovirus; CCL21; Cancer immunotherapy; Chemokines; IL-15