Pathways involved in vanadate-induced root hair formation in Arabidopsis.
Root hair formation is controlled by environmental signals. We found significantly increased Arabidopsis root hair density and length in response to low-dose vanadate (V). Reactive oxygen species (ROS) production was induced with V treatment. We investigated the possible role of NADPH oxidase in altering root system architecture induced by V by using diphenylene iodonium (DPI), an inhibitor of NADPH oxidase, and an NADPH oxidase mutant (rhd2/AtrbohC). NADPH oxidase was involved in root hair elongation induced by V. As well, ethylene receptor (ETR1) and ROOT HAIR DEFECTIVE (RHD6) participated in inducing root hair formation induced by V. Furthermore, the kinase inhibitors, genistein (tyrosine kinase inhibitor) and K252a (ser/thr kinase inhibitor), and a phosphatase inhibitor, cantharidin (ser/thr phosphatase inhibitor), suppressed root hair formation induced by V. To elucidate the regulation of gene expression in response to V, we investigated transcriptional changes in roots by microarray assay. Exposure to V triggered changes in transcript levels of genes related to cell wall formation, ROS activity and signaling. Several genes involved in root hair formation were also regulated.
Department of Life Sciences, National Cheng Kung University, No. 1 University Road 701, Tainan, Taiwan.
Chung-Yi
CY
Lin
Li-Yao
LY
Huang
Wen-Chang
WC
Chi
Tsai-Lien
TL
Huang
Tatsuo
T
Kakimoto
Chang-Rong
CR
Tsai
Hao-Jen
HJ
Huang
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D002193:Cantharidin / Q000494:pharmacology; D002473:Cell Wall / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017930:Genes, Reporter; D019833:Genistein / Q000494:pharmacology; D009154:Mutation; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D009861:Onium Compounds / Q000494:pharmacology; D010641:Phenotype; D010766:Phosphorylation; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings; D015398:Signal Transduction / Q000187:drug effects*; D014638:Vanadates / Q000494:pharmacology*
D029681:Arabidopsis Proteins; D004791:Enzyme Inhibitors; D009861:Onium Compounds; D017382:Reactive Oxygen Species; D014638:Vanadates; C007517:diphenyleneiodonium; D019833:Genistein; D019255:NADPH Oxidases; D002193:Cantharidin