Validation of free flow electrophoresis as a novel plasma and serum processing and fractionation method in biobanking.
Free flow electrophoresis (FFE) is a fractionation method, based on isoelectric focusing (IEF). We validate the reproducibility of the method and show that it can be applied by biobanks in order to fractionate fluid biospecimens efficiently and reproducibly and to facilitate downstream proteomic applications. We also propose a simple method allowing researchers to assess the reproducibility of each FFE run.
1 Biobanque de Picardie, Saleux, France .
Gwenaelle
G
Gaillard
Jean-Pierre
JP
Trezzi
Fotini
F
Betsou
D018070:Biological Specimen Banks; D005591:Chemical Fractionation / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results