CDX2 gene expression in acute lymphoblastic leukemia.
CDX genes are classically known as regulators of axial elongation during early embryogenesis. An unsuspected role for CDX genes has been revealed during hematopoietic development. The CDX gene family member CDX2 belongs to the most frequent aberrantly expressed proto-oncogenes in human acute leukemias and is highly leukemogenic in experimental models. We used reversed transcriptase polymerase chain reaction (RT-PCR) to determine the expression level of CDX2 gene in 30 pediatric patients with acute lymphoblastic leukemia (ALL) at diagnosis and 30 healthy volunteers. ALL patients were followed up to detect minimal residual disease (MRD) on days 15 and 42 of induction. We found that CDX2 gene was expressed in 50% of patients and not expressed in controls. Associations between gene expression and different clinical and laboratory data of patients revealed no impact on different findings. With follow up, we could not confirm that CDX2 expression had a prognostic significance.
Clinical Pathology & Hematology, Faculty of Medicine, Cairo University, Egypt.
Hanaa H
HH
Arnaoaut
Clinical Pathology & Hematology, Faculty of Medicine, Cairo University, Egypt.
Doha A
DA
Mokhtar
Clinical Pathology & Hematology, Faculty of Medicine, Cairo University, Egypt. Electronic address: Rania_sami1976@yahoo.com.
Rania M
RM
Samy
Oncology Unit of National Cancer Institute, Cairo University, Egypt.
Sahar A
SA
Khames
Clinical Pathology & Hematology, Faculty of Medicine, Cairo University, Egypt.
Shereen A
SA
Omar
D000293:Adolescent; D000071616:CDX2 Transcription Factor; D002648:Child; D002675:Child, Preschool; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008297:Male; D018365:Neoplasm, Residual / Q000235:genetics* / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D011379:Prognosis
D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; D018398:Homeodomain Proteins
Acute lymphoblastic leukemia; CDX2; Minimal residual disease; RT-PCR