Transcriptional regulation of CD4 gene expression in porcine kidney epithelial cells by virus-like double-stranded RNA and DNA methyltransferase inhibitor.
The effects of virus-like double-stranded RNA (dsRNA, PolyI:C) and DNA methyltransferase inhibitor (Aza-CdR) on CD4 gene expression were investigated in a porcine kidney cell line (PK15). We found that expression levels of TLR3 and IFNαwere significantly upregulated by PolyI:C, compared to the untreated PK15 cells, which shows that PolyI:C successfully mimics viral infection in PK15 cells. We also found that PolyI:C (10 μg/ml) and/or Aza-CdR (5 μM) significantly induces DNA demethylation of porcine CD4, promoting the binding of NF-κB to the CpG site on the CD4 promoter and activating expression of CD4. These data help clarify the regulatory mechanism of DNA methylation of the CD4 gene in non-immune cell response to virus replication. Further study is warranted to identify CD4 gene expression regulated by DNA methylation and live virus infection.
National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing, China.
X S
XS
Wang
State Key Laboratory for Animal Nutrition, Key Laboratory for Domestic Animal Genetic Resources and Breeding of the Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences.
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H
Ao
National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing, China.
L W
LW
Zhai
State Key Laboratory for Animal Nutrition, Key Laboratory for Domestic Animal Genetic Resources and Breeding of the Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences.
L J
LJ
Bai
State Key Laboratory of Agrobiotechnology, Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing, China.
W Y
WY
He
National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing, China wangchuduan@qq.com.
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CD
Wang
National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing, China.
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Y
Yu
D000818:Animals; D015704:CD4 Antigens / Q000096:biosynthesis*; D019175:DNA Methylation / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology; D005786:Gene Expression Regulation; D007668:Kidney / Q000166:cytology / Q000187:drug effects; D008780:Methyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012330:RNA, Double-Stranded / Q000235:genetics*; D013552:Swine; D014779:Virus Replication / Q000235:genetics
D015704:CD4 Antigens; D004791:Enzyme Inhibitors; D012330:RNA, Double-Stranded; D008780:Methyltransferases