DNA molecular beacon-based plastic biochip: a versatile and sensitive scanometric detection platform.
In this paper, we report a novel DNA molecular beacon (MB)-based plastic biochip platform for scanometric detection of a range of analytical targets. Hairpin DNA strands, which are dually modified with amino and biotin groups at their two ends are immobilized on a disposable plastic (polycarbonate) substrate as recognition element and gold nanoparticle-assisted silver-staining as signal reading protocol. Initially, the immobilized DNA probes are in their folded forms; upon target binding the hairpin secondary structure of the probe strand is "forced" open (i.e., converted to the unfolded state). Nanogold-streptavidin conjugates can then bind the terminal biotin groups and promote the deposition of rather large silver particles which can be either directly visualized or quantified with a standard flatbed scanner. We demonstrate that with properly designed probe sequences and optimized preparation conditions, a range of molecular targets, such as DNA strands, proteins (thrombin) and heavy metal ions (Hg(2+)), can be detected with high sensitivity and excellent selectivity. The detection can be done in both standard physiological buffers and real world samples. This constitutes a platform technology for performing rapid, sensitive, cost-effective, and point-of-care (POC) chemical analysis and medical diagnosis.
Department of Chemistry, Beijing Normal University , Beijing 100875, P. R. China.
Xiaoli
X
Shi
Jing
J
Wen
Yunchao
Y
Li
Yue
Y
Zheng
Jianjun
J
Zhou
Xiaohong
X
Li
Hua-Zhong
HZ
Yu
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D010969:Plastics; D013499:Surface Properties
D015342:DNA Probes; D010969:Plastics; D004247:DNA
DNA molecular beacon; medical diagnosis; plastic biochip; scanometric detection; target-induced conformation switching