Recrystallization of dihydromyricetin from Ampelopsis grossedentata and its anti-oxidant activity evaluation.
A fast and efficient method for purification of dihydromyricetin (3,5,7,3',4',5'-six hydroxy-2,3-dihydro flavonol; DMY) from Ampelopsis grossedentata was created by crystallization eight times at 25°C, and a purity of 98% was finally achieved. The purified DMY exhibited high oxygen radical absorbance capacity (ORAC) (30.21 μmol Trolox equiv/mg) and strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half-maximal inhibitory concentration [IC50]=0.235 μg/mL). The addition of DMY could also effectively attenuate 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced human erythrocyte hemolysis and cupric chloride (CuCl2)-induced human plasma lipid peroxidation via inhibition of intracellular reactive oxygen species (ROS) generation. It was also found that DMY (>12 μg/mL) treatment significantly inhibited intracellular malondialdehyde (MDA) formation. Meanwhile, DMY treatment significantly inhibited the obvious increase of anti-oxidant enzymes levels (superoxide dismutase [SOD]; glutathione peroxidase [GPX], and catalase [CAT]) induced by AAPH radicals, suggesting that stress defense mechanisms are associated with protection of DMY against intracellular oxidation.
Department of Food Science and Technology, South China University of Technology , Guangzhou, Guangdong, China .
Wenzhen
W
Liao
Zhengxiang
Z
Ning
Ling
L
Ma
Xueru
X
Yin
Qingyi
Q
Wei
Erdong
E
Yuan
Jiguo
J
Yang
Jiaoyan
J
Ren
D000328:Adult; D000578:Amidines / Q000633:toxicity; D032421:Ampelopsis / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology; D044948:Flavonols / Q000737:chemistry* / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D000578:Amidines; D000975:Antioxidants; D044948:Flavonols; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; C046728:2,2'-azobis(2-amidinopropane); D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C472036:dihydromyricetin