Seroprevalence of Neospora caninum in cats from the Czech Republic.
Sera of 414 cats coming from different parts of the Czech Republic were tested for N. caninum antibodies. Sera samples were collected during years 2002-2011. N. caninum antibodies were detected by a commercial competitive-inhibition enzyme-linked immunosorbent assay (cELISA) with cut off ≥30% inhibition. Samples positive in cELISA were confirmed by an indirect fluorescence antibody test (IFAT); titre ≥50 was considered positive. In total, 137 (33%) cats reacted positively in cELISA; N. caninum antibodies in IFAT were detected in 16 (3.86%) cats with titres 50 and 100. In 6 cats, positive for N. caninum antibodies, T. gondii antibodies were also detected by IFAT. It is the first report of N. caninum antibodies in domestic cats from the Czech Republic and third report in Europe.
Department of Virology and Serology, State Veterinary Institute Prague, Sidlistni 136/24, 165 03, Prague 6, Czech Republic.
Kamil
K
Sedlák
Eva
E
Bartova
Tereza
T
Machacova
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D002371:Cat Diseases / Q000453:epidemiology* / Q000382:microbiology*; D002415:Cats; D003048:Coccidiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D018153:Czech Republic / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D018688:Neospora / Q000276:immunology*; D016036:Seroepidemiologic Studies
D000908:Antibodies, Fungal