A quantum dot-based microRNA nanosensor for point mutation assays.
We have developed a quantum dot-based microRNA nanosensor for point mutation assays using primer generation-mediated rolling circle amplification. The proposed method exhibits high sensitivity with a detection limit of as low as 50.9 aM and a large dynamic range of 7 orders of magnitude from 0.1 fM to 1 nM. Importantly, this method can be further applied to analyze the point mutation of mir-196a2 in the lung tissues of non small-cell lung cancer patients.
Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. zhangcy@siat.ac.cn.
Ya-ping
YP
Zeng
Guichi
G
Zhu
Xiao-yun
XY
Yang
Jun
J
Cao
Zhi-liang
ZL
Jing
Chun-yang
CY
Zhang
D001681:Biological Assay; D015374:Biosensing Techniques; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D006801:Humans; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D061848:Optical Imaging; D017354:Point Mutation; D045663:Quantum Dots
C542543:MIRN196 microRNA, human; D035683:MicroRNAs