Quorum-sensing gene luxS regulates flagella expression and Shiga-like toxin production in F18ab Escherichia coli.
To investigate the effect of the luxS gene on the expression of virulence factors in Shiga-like toxin producing and verotoxin-producing Escherichia coli, the luxS gene from E. coli 107/86 (wild type, O139:H1:F18ab, Stx2e) was deleted. The successful deletion of luxS was confirmed by bioluminescence assays. The luxS deletion mutant exhibited changed flagella-related phenotypes, like impaired expression of flagella, decreased flagella motility, reduced biofilm formation, and reduced ability to induce pro-immunity response in host cells, which were restored after complementation with the intact luxS gene. The mutant strain also displayed attenuated production of Stx2e. This study provides new information to the crucial function of luxS in regulating Shiga-like toxin producing E. coli virulence.
a College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, People's Republic of China.
Yang
Y
Yang
Mingxu
M
Zhou
Huayan
H
Hou
Jun
J
Zhu
Fenghua
F
Yao
Xinjun
X
Zhang
Xiaofang
X
Zhu
Philip R
PR
Hardwidge
Guoqiang
G
Zhu
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D013437:Carbon-Sulfur Lyases / Q000235:genetics* / Q000502:physiology; D002522:Chlorocebus aethiops; D005407:Flagella / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008163:Luminescent Measurements; D053038:Quorum Sensing / Q000235:genetics*; D022603:Shiga Toxins / Q000096:biosynthesis* / Q000235:genetics; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D014709:Vero Cells; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D022603:Shiga Toxins; D037521:Virulence Factors; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria
Escherichia coli; détection du quorum; flagella; flagelles; luxS; quorum sensing