Development of silica gel-supported modified macroporous chitosan membranes for enzyme immobilization and their characterization analyses.
The present work was aimed at developing stability enhanced silica gel-supported macroporous chitosan membrane for immobilization of enzymes. The membrane was surface modified using various cross-linking agents for covalent immobilization of enzyme Bovine serum albumin. The results of FT-IR, UV-vis, and SEM analyses revealed the effect of cross-linking agents and confirmed the formation of modified membranes. The presence of silica gel as a support could provide a large surface area, and therefore, the enzyme could be immobilized only on the surface, and thus minimized the diffusion limitation problem. The resultant enzyme immobilized membranes were also characterized based on their activity retention, immobilization efficiency, and stability aspects. The immobilization process increased the activity of immobilized enzyme even higher than that of total (actual) activity of native enzyme. Thus, the obtained macroporous chitosan membranes in this study could act as a versatile host for various guest molecules.
Graduate Institute of Environmental Engineering, National Central University, Chung-Li, Taoyuan County, 320, Taiwan.
Wen-Yi
WY
Yang
Munusamy
M
Thirumavalavan
Madasamy
M
Malini
Gurusamy
G
Annadurai
Jiunn-Fwu
JF
Lee
D048271:Chitosan / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D058428:Silica Gel / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
D004800:Enzymes, Immobilized; D058428:Silica Gel; D048271:Chitosan