Cadmium-tolerant bacteria induce metal stress tolerance in cereals.
Cadmium usually hampers plant growth, but bacterial inoculation may improve stress tolerance in plants to Cd by involving various mechanisms. The objective was to characterize and identify bacteria that improve plant growth under Cd stress and reduce Cd uptake. Cadmium-tolerant bacteria were isolated from rhizosphere soil, which was irrigated with tannery effluent, and six strains were selected as highly tolerant to Cd, showing minimum inhibitory concentration as 500 mg L(-1) or 4.45 mmol L(-1). These strains were identified by 16S rRNA gene analysis and functional analysis in regard to plant growth promotion characteristics. To determine their effect on cereal growth under Cd stress, seeds were inoculated with these strains individually and grown in soil contaminated with three Cd levels (0, 40 and 80 mg kg(-1)). Biomass production, relative water content (RWC), electrolyte leakage (ELL) and tissue Cd concentration were measured. Biomass of both cereals was inhibited strongly when exposed to Cd; however, bacterial inoculation significantly reduced the suppressive effect of Cd on cereal growth and physiology. The bacterial isolates belonged to the genera Klebsiella, Stenotrophomonas, Bacillus and Serratia. Maize was more sensitive than wheat to Cd. Klebsiella sp. strain CIK-502 had the most pronounced effects in promoting maize and wheat growth and lowering Cd uptake under Cd stress.
Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, 38040, Pakistan, iffises@yahoo.com.
Iftikhar
I
Ahmad
Muhammad Javed
MJ
Akhtar
Zahir Ahmad
ZA
Zahir
Muhammad
M
Naveed
Birgit
B
Mitter
Angela
A
Sessitsch
D000222:Adaptation, Physiological; D001407:Bacillus / Q000187:drug effects / Q000235:genetics; D001419:Bacteria / Q000187:drug effects; D001673:Biodegradation, Environmental; D002104:Cadmium / Q000494:pharmacology*; D007709:Klebsiella / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D012705:Serratia / Q000187:drug effects / Q000235:genetics; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000494:pharmacology*; D020588:Stenotrophomonas / Q000187:drug effects / Q000235:genetics; D014908:Triticum / Q000187:drug effects / Q000254:growth & development*; D003313:Zea mays / Q000187:drug effects / Q000254:growth & development*
D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium