Cryopreservation of canine epididymal sperm using ACP-106c and TRIS.
The objective was to cryopreserve sperm recovered from the canine epididymal cauda immediately after an orchiectomy. The sperm was stored for 12h at 4 °C using ACP-106c and TRIS as extenders. Sixty adult male dogs were used. The testis-epididymis complex (TEC) was removed, immersed in 0.9% saline and transported to the laboratory. The 60 TEC were divided into groups according to the 4 °C cooling time (0 h or 12 h) and according to the extender used for sperm recovery (ACP-106c or TRIS), forming 4 experimental groups: G0h-ACP, G12h-ACP, G0h-TRIS and G12h-TRIS. The sperm were recovered from the epididymal cauda using the retrograde flow technique. Next, 1.0 mL of ACP-106c or 1.0 mL of TRIS (preheated to 37 °C for 5 min) was added to the sperm of each epididymis. One week later, the sperm was thawed at 37 °C for 1 min, and its morphology, functionality and total and progressive sperm motilities were analyzed. Other parameters were obtained by Computer Assisted Semen Analysis (CASA). The data were submitted to multivariate analysis of variance (MANOVA) (P<0.05). The total motility values were 52.17 ± 1.78 and 49.8 ± 1.93 for groups G0h-ACP and G12h-ACP and 50.7 ± 2.06 and 43.90 ± 2.51 for groups G0h-TRIS and G12h-TRIS, respectively. A decrease in total sperm motility was observed after 12h of cooling for both extenders (P<0.05). ACP-106c can be used as an extender for freezing canine epididymal sperm, and the freezing procedure must be performed immediately after sperm recovery.
Laboratory of Carnivore Reproduction, School of Veterinary Medicine, State University of Ceara, Av. 1700, CEP Fortaleza, CE, Brazil.
Antônio Cavalcante
AC
Mota Filho
Laboratory of Carnivore Reproduction, School of Veterinary Medicine, State University of Ceara, Av. 1700, CEP Fortaleza, CE, Brazil.
Herlon Victor Rodrigues
HV
Silva
Laboratory of Swine Semen Technology, School of Veterinary Medicine, State University of Ceara, Av. 1700, CEP Fortaleza, CE, Brazil.
Thalles Gothardo Pereira
TG
Nunes
Laboratory of Carnivore Reproduction, School of Veterinary Medicine, State University of Ceara, Av. 1700, CEP Fortaleza, CE, Brazil.
Mírley Barbosa
MB
de Souza
Laboratory of Carnivore Reproduction, School of Veterinary Medicine, State University of Ceara, Av. 1700, CEP Fortaleza, CE, Brazil.
Luana Azevedo
LA
de Freitas
Faculdade de Zootecnia, Federal University of Ceara, Campus do Pici, Av. Mister Hull, 2977, CEP 60021-970 Fortaleza, CE, Brazil.
Airton Alencar
AA
de Araújo
Laboratory of Carnivore Reproduction, School of Veterinary Medicine, State University of Ceara, Av. 1700, CEP Fortaleza, CE, Brazil. Electronic address: lucia.daniel.machado@hotmail.com.
Lúcia Daniel Machado
LD
da Silva
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000737:chemistry; D004285:Dogs; D004822:Epididymis / Q000166:cytology / Q000601:surgery; D005615:Freezing; D008297:Male; D009919:Orchiectomy; D028321:Plant Preparations / Q000737:chemistry; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*; D013737:Testis / Q000601:surgery; D014325:Tromethamine / Q000737:chemistry
C570992:ACP-106C; D003451:Cryoprotective Agents; D028321:Plant Preparations; D014325:Tromethamine
Cryopreservation; Dog; Epididymis; Sperm