Rab35 is translocated from Arf6-positive perinuclear recycling endosomes to neurite tips during neurite outgrowth.
Small GTPase Rab35 is a key regulator of neurite outgrowth, and its activation dramatically enhances nerve growth factor (NGF)-induced neurite outgrowth. We recently reported finding that Rab35 and its effector molecules recruit EHD1, a facilitator of vesicle formation, to Arf6-positive perinuclear recycling endosomes (hereafter simply referred to as recycling endosomes) in response to NGF stimulation. Although Rab35 is likely to promote the formation of transport vesicles from recycling endosomes that contributes to neurite outgrowth, the destination of the vesicles during neurite outgrowth remains unknown. Here we report finding that Rab35 is translocated from recycling endosomes to neurite tips in a late phase of NGF stimulation. We found that Rab35 immunofluorescence signals accumulated at recycling endosomes during the first 6 h, i.e., the early phase of NGF stimulation and then translocated to neurite tips during the late phase of NGF stimulation (i.e., >6 h to <36 h after NGF stimulation). These findings suggest that Rab35 regulates membrane trafficking from recycling endosomes to neurite tips during neurite outgrowth.
Laboratory of Membrane Trafficking Mechanisms; Department of Developmental Biology and Neurosciences; Graduate School of Life Sciences; Tohoku University; Sendai, Miyagi, Japan.
Hotaka
H
Kobayashi
Laboratory of Membrane Trafficking Mechanisms; Department of Developmental Biology and Neurosciences; Graduate School of Life Sciences; Tohoku University; Sendai, Miyagi, Japan.
Kan
K
Etoh
Laboratory of Membrane Trafficking Mechanisms; Department of Developmental Biology and Neurosciences; Graduate School of Life Sciences; Tohoku University; Sendai, Miyagi, Japan.
Mitsunori
M
Fukuda
D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D000818:Animals; D011992:Endosomes / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D022161:Transport Vesicles / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
D000089982:ADP-Ribosylation Factor 6; D020932:Nerve Growth Factor; C575759:Rab35 protein, rat; D020727:ADP-Ribosylation Factors; C000715349:Arf6 protein, rat; D020691:rab GTP-Binding Proteins
Arf6; Rab35; neurite outgrowth; recycling endosome