The accessory gas vesicle protein GvpM of haloarchaea and its interaction partners during gas vesicle formation.
Gas vesicles consist predominantly of the hydrophobic GvpA and GvpC, and the accessory proteins GvpF through GvpM are required in minor amounts during formation. GvpM and its putative interaction partners were investigated. GvpM interacted with GvpH, GvpJ and GvpL, but not with GvpG. Interactions were also observed in vivo in Haloferax volcanii transformants using Gvp fusions to the green fluorescent protein smGFP. Cells producing the hydrophobic M(GF)P contained a single fluorescent aggregate per cell, whereas cells containing L(GFP) or H(GFP) were fully fluorescent. The soluble L(GFP) formed stable co-aggregates with GvpM in L(GFP)M transformants, but the presence of GvpH resulted in the absence of M(GF)P foci in HM(GFP) transformants. Substitution- and deletion mutants of GvpM determined functionally important amino acids (aa). Substitution of a polar by a non-polar aa in the N-terminal region of GvpM had no effect, whereas a substitution of a non-polar by a polar aa in this region inhibited gas vesicle formation in transformants. Substitutions in region 44-48 of GvpM strongly reduced the number of gas vesicles, and deletions at the N-terminus resulted in Vac(-) transformants. Gas vesicle morphology was not affected by any mutation, implying that GvpM is required during initial stages of gas vesicle assembly.
Mikrobiologie und Archaea, Fachbereich Biologie, Technische Universität Darmstadt, Schnittspahnstrasse 10, 64287, Darmstadt, Germany.
Stella
S
Tavlaridou
Kerstin
K
Winter
Felicitas
F
Pfeifer
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D019622:Haloferax volcanii / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055049:Salt Tolerance
D019843:Archaeal Proteins; D011506:Proteins; C038524:gas vesicle protein