HepG2.2.15 as a model for studying cell protrusion and migration regulated by S100 proteins.
Much of the difficulty in elucidating the precise function of S100 protein family has been attributed to functional redundancy and compensation by its conserved family members. In this study, we showed that seven S100 family members were almost totally undetectable in HepG2.2.15 cells, while all of them were highly expressed in its parental HepG2 cells. Re-expression of S100 proteins in HepG2.2.15 cells can partially rescue their defects in cell protrusion and migration through the regulation of cytoskeletons and adhesions. Thus, HepG2.2.15 can serve as a useful model for studying cell protrusion and migration regulated by S100 proteins.
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China.
Shiying
S
Liu
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China.
Rui
R
Zhou
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China.
Jian
J
Zhong
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China.
Chunlai
C
Nie
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China.
Zhu
Z
Yuan
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, PR China.
Liangxue
L
Zhou
Nankai University School of Medicine, Tianjin, PR China.
Na
N
Luo
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China. Electronic address: chtwang@163.com.
Chunting
C
Wang
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China. Electronic address: aipingtong@scu.edu.cn.
Aiping
A
Tong
D049108:Cell Enlargement; D002465:Cell Movement / Q000502:physiology*; D056945:Hep G2 Cells / Q000145:classification / Q000473:pathology* / Q000502:physiology*; D006801:Humans; D009418:S100 Proteins / Q000502:physiology*
D009418:S100 Proteins
Cell migration; Cell protrusion; Cytoskeleton; Hepg2.2.15 cells; S100 proteins