Down-regulation of miR-3928 promoted osteosarcoma growth.
BACKGROUND
Osteosarcoma is the most common primary bone malignancy in children and young adults. Most failures of osteosarcoma treatment were due to resistance to chemotherapy. Development of new therapy required elucidation underlying molecular mechanism. Many miRNAs have been proved to be involved in the pathogenesis of osteosarcoma.


METHODS
MiR-3928 expression level was assayed by qRT-PCR. MiRNA mimics or ASO were transfected for up-regulation or down-regulation of miR-3928 expression. Cell proliferation was assayed by formazan test. Apoptosis and cell cycle were assayed by FACS. MiR-3928 targeted genes were predicated by bioinformatics algorithm (TargetScanHuman). The correlation between targeted gene and miR-3928 was analyzed by Pearson's correlation coefficient analysis.


RESULTS
MiR-3928 was down-regulated in osteosarcoma tissues. Over-expression of miR-3928 inhibited tumor growth, induced cell apoptosis, increased the percent of cells in G1 phrase and decreased the percent of cells in S phrase. Down-regulation of miR-3928 promoted cell proliferation. ERBB3, IL-6R and CDK6 may be the targeted genes of miR-3928.


CONCLUSIONS
Down-expression of miR-3928 in osteosarcoma promoted tumor growth by targeting ERBB3, IL-6R and CDK6. MiR-3928 may be a potential therapy target worth further investigation.
Department of Orthopedics of Jinling Hospital, Nanjing University, School of Medicine, Nanjing, China.
Haidong
H
Xu
Xiaozhou
X
Liu
Jianning
J
Zhao
D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D002453:Cell Cycle; D049109:Cell Proliferation / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D014407:Tumor Cells, Cultured; D055815:Young Adult
D035683:MicroRNAs