Americanin B protects cultured human keratinocytes against oxidative stress by exerting antioxidant effects.
We evaluated the cytoprotective effects of americanin B, a lignan compound, against hydrogen peroxide (H2O2)-induced cell damage. Americanin B decreased the level of DPPH radicals, superoxide anions, hydroxyl radicals, and intracellular reactive oxygen species. Americanin B also attenuated DNA damage induced by H2O2 treatment, as shown by the inhibition of formation of comet tails, indicative of DNA strand breakage, and prevented the oxidation of protein and peroxidation of lipid, as determined by protein carbonyls and 8-isoprostane. Furthermore, americanin B protected against H2O2-induced apoptotic cell death, as determined by a reduction in the numbers of apoptotic bodies stained with Hoechst 33342. These findings suggest that americanin B protects cells against oxidative damage by exerting antioxidant effects and inhibiting apoptosis.
School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju, 690-756, Korea.
Jian
J
Zheng
Mei Jing
MJ
Piao
Ki Cheon
KC
Kim
Cheng Wen
CW
Yao
Ji Won
JW
Cha
Seong Min
SM
Kim
Chang Lim
CL
Hyun
Sungwook
S
Chae
Yong Seok
YS
Ahn
Jin Won
JW
Hyun
D000975:Antioxidants / Q000494:pharmacology*; D001562:Benzimidazoles; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D020552:Comet Assay; D004146:Dioxanes / Q000494:pharmacology*; D005609:Free Radicals / Q000032:analysis; D006801:Humans; D017665:Hydroxyl Radical / Q000032:analysis; D015603:Keratinocytes / Q000737:chemistry / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000032:analysis; D013481:Superoxides / Q000032:analysis
D000975:Antioxidants; D001562:Benzimidazoles; D004396:Coloring Agents; D004146:Dioxanes; D005609:Free Radicals; D017382:Reactive Oxygen Species; C000598877:americanin B; D013481:Superoxides; D017665:Hydroxyl Radical; C017807:bisbenzimide ethoxide trihydrochloride