Multicapillary SDS-gel electrophoresis for the analysis of fluorescently labeled mAb preparations: a high throughput quality control process for the production of QuantiPlasma and PlasmaScan mAb libraries.
Molecular heterogeneity of mAb preparations is the result of various co- and post-translational modifications and to contaminants related to the production process. Changes in molecular composition results in alterations of functional performance, therefore quality control and validation of therapeutic or diagnostic protein products is essential. A special case is the consistent production of mAb libraries (QuantiPlasma™ and PlasmaScan™) for proteome profiling, quality control of which represents a challenge because of high number of mAbs (>1000). Here, we devise a generally applicable multicapillary SDS-gel electrophoresis process for the analysis of fluorescently labeled mAb preparations for the high throughput quality control of mAbs of the QuantiPlasma™ and PlasmaScan™ libraries.
BioSystems International Kft, Debrecen, Hungary.
Andrea
A
Székely
Akos
A
Szekrényes
Márta
M
Kerékgyártó
Attila
A
Balogh
János
J
Kádas
József
J
Lázár
András
A
Guttman
István
I
Kurucz
László
L
Takács
D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000379:methods / Q000592:standards*; D006801:Humans; D019151:Peptide Library; D011786:Quality Control; D012709:Serum Albumin; D000075462:Serum Albumin, Human
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D006023:Glycoproteins; D019151:Peptide Library; C114780:Quantison Depot; D012709:Serum Albumin; D000075462:Serum Albumin, Human
Capillary electrophoresis; Glycoform; Quality control; SDS; mAb proteomics