Overexpression of tomato GDP-L-galactose phosphorylase gene in tobacco improves tolerance to chilling stress.
The overexpression of tomato GDP- l -galactose phosphorylase gene enhanced tolerance to chilling stress and reduced photoinhibition of photosystems I and II in transgenic tobacco. Chilling stress is a crucial factor that limits the geographical distribution and yield of chilling-sensitive plants. Ascorbate (AsA) protects plants by scavenging reactive oxygen species and reduces photoinhibition by promoting the conversion of violaxanthin to zeaxanthin in the xanthophyll cycle to dissipate excess excitation energy. Possible mechanisms of AsA for plant photoprotection under chilling stress were investigated by isolating the tomato GDP-L-galactose phosphorylase gene (SlGGP) and producing transgenic tobacco plants with overexpression of SlGGP. The transgenic plants subjected to chilling stress accumulated less H(2)O(2), demonstrated lower levels of ion leakage and malondialdehyde, and acquired higher net photosynthetic rate, higher maximum photochemical efficiency of PSII, and higher D1 protein content compared with the wild-type (WT) plants. The transgenic plants subjected to chilling stress also showed higher GDP-L-galactose phosphorylase activity, increased AsA content as well as ascorbate peroxidase and oxidizable P700 activities than WT plants. Thus, SlGGP overexpression is crucial in promoting AsA synthesis and alleviating photoinhibition of two photosystems.
College of Life Science, Dezhou University, Dezhou, 253023, Shandong, People's Republic of China.
Liyan
L
Wang
Xia
X
Meng
Dongyue
D
Yang
Nana
N
Ma
Guodong
G
Wang
Qingwei
Q
Meng
D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism*; D003080:Cold Temperature; D005690:Galactose / Q000378:metabolism; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D008027:Light; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D018384:Oxidative Stress; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology; D045331:Photosystem I Protein Complex / Q000502:physiology; D045332:Photosystem II Protein Complex / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000201:enzymology / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D013312:Stress, Physiological; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics / Q000502:physiology / Q000528:radiation effects
D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D010744:Phosphoric Monoester Hydrolases; D001205:Ascorbic Acid; D005690:Galactose