Ionic liquid-based one-step micellar extraction of multiclass polar compounds from hawthorn fruits by ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry.
An ionic liquid (IL)-based one-step micellar extraction procedure was developed for the extraction of multiclass polar analytes (protocatechuic acid, chlorogenic acid, epicatechin, hyperoside, isoquercitrin, quercetin) from hawthorn fruits and their determination using ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF/MS). Compared to conventional organic solvent extractions, this newly proposed method was much easier, more sensitive, environmentally friendly, and effective as well. Several important parameters influencing the micellar extraction efficiency are discussed, such as selection of ILs, surfactant concentration, and extraction time. Under the optimal conditions, good linearity was achieved for each analyte with correlation coefficients (r(2)) ranging from 0.9934 to 0.9999, and the recovery values ranged from 89.3 to 106% with relative standard deviations lower than 5.5%. Results suggest that the IL-based one-step micellar extraction could be an alternative and promising means in future food analysis.
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University , Hangzhou 310036, China.
Shuai-Shuai
SS
Hu
Ling
L
Yi
Xing-Ying
XY
Li
Jun
J
Cao
Li-Hong
LH
Ye
Wan
W
Cao
Jian-Hua
JH
Da
Han-Bin
HB
Dai
Xiao-Juan
XJ
Liu
D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D027825:Crataegus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D052578:Ionic Liquids; D010936:Plant Extracts