Gene disruption using zinc finger nuclease technology.
Zinc finger nucleases are reagents that induce DNA double-strand breaks at specific sites that can be repaired by nonhomologous end joining, inducing alterations in the genome. This strategy has enabled highly efficient gene disruption in numerous cell types and model organisms opening a door for new therapeutic applications. Here, we describe the disruption of CD147/basigin by this technique in a human cancer cell line.
Surgical Sciences Domain, Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal.
Sara
S
Granja
Ibtissam
I
Marchiq
Fátima
F
Baltazar
Jacques
J
Pouysségur
D051926:Basigin / Q000235:genetics; D045744:Cell Line, Tumor; D003850:Deoxyribonuclease I / Q000737:chemistry* / Q000378:metabolism*; D005434:Flow Cytometry; D055786:Gene Knockout Techniques / Q000379:methods*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D014170:Transformation, Genetic; D016335:Zinc Fingers
D051926:Basigin; D003850:Deoxyribonuclease I