Growth inhibition, cell-cycle alteration and apoptosis in stimulated human peripheral blood lymphocytes by multiwalled carbon nanotube buckypaper.
OBJECTIVE
This study was designed to investigate the cytotoxicity of multiwalled carbon nanotube buckypaper (BP) in stimulated human peripheral blood lymphocytes. Materials & methods & results: BP treatment led to a delay in the cell growth, as proven by a minor increase in the cell number over time relative to that seen in untreated cells, assessed by trypan blue, resazurin and neutral red assays. The analysis of cell-cycle profile, by propidium iodide staining, indicated that BP treatment blocked cell-cycle progression by arresting cells at the G0/G1 phase. Moreover, increased apoptosis was also recorded by Annexin V-fluorescein isothiocyanate/propidium iodide staining.


CONCLUSIONS
The results presented here demonstrate an inhibitor effect of BP on cell growth that was likely through cytostatic and cytotoxic events.
National Research Council (CNR) - Institute for Electromagnetic Sensing of Environment, Via Diocleziano 328, 80124 Naples, Italy.
Olga
O
Zeni
Anna
A
Sannino
Stefania
S
Romeo
Federico
F
Micciulla
Stefano
S
Bellucci
Maria Rosaria
MR
Scarfi
D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure
D037742:Nanotubes, Carbon
apoptosis; biocompatibility; cell-cycle arrest; human peripheral blood lymphocyte; multiwalled carbon nanotube buckypaper