Plasma semicarbazide-sensitive amine oxidase activity in type 1 diabetes is related to vascular and renal function but not to glycaemia.
OBJECTIVE
Associations of semicarbazide-sensitive amine oxidase (SSAO) activity with renal and vascular function, oxidative stress, glycaemia and diabetes complications were determined.


METHODS
Plasma SSAO activity in 94 type 1 diabetes (T1DM) patients, including 34 with microvascular complications T1DM CX[+], and in 96 healthy subjects (CON) was measured by production of benzaldehyde using high-performance liquid chromatography (HPLC).


RESULTS
SSAO activity (mean ± SD) was greater in T1DM than in CON (1049 ± 294 vs 749 ± 204 mU/L; p < 0.00001) and was higher in T1DM CX[+] vs complication-free DM subjects (1148 ± 313 mU/L vs 982 ± 269 mU/L; p = 0.01). In T1DM, SSAO activity correlated with renal dysfunction [estimated glomerular filtration rate (eGFR): r = -0.44; p = 0.0001; cystatin C: r = 0.47; p = 0.0001] and markers of inflammation [soluble vascular cell adhesion molecule-1 (sVCAM-1): r = 0.41, p = 0.0001; soluble intercellular adhesion molecule-1 (sICAM-1): r = 0.33, p = 0.002] and was inversely related to small artery elasticity (SAE) (r = -0.23, p = 0.03). In CON, SSAO activity correlated with HbA1c (r = 0.26; p = 0.02).


CONCLUSIONS
In T1DM, SSAO activity correlates with renal dysfunction, but not with glycaemia, and may promote vascular inflammation and be a therapeutic target.
NHMRC Clinical Trials Centre, University of Sydney, Camperdown, NSW, Australia Department of Medicine, St Vincent's Hospital, University of Melbourne, Melbourne, Australia andrzej.januszewski@ctc.usyd.edu.au.
Andrzej S
AS
Januszewski
Department of Medicine, St Vincent's Hospital, University of Melbourne, Melbourne, Australia.
Nick
N
Mason
Department of Medicine, St Vincent's Hospital, University of Melbourne, Melbourne, Australia.
Connie S
CS
Karschimkus
Department of Medicine, St Vincent's Hospital, University of Melbourne, Melbourne, Australia.
Kevin G
KG
Rowley
Department of Medicine, St Vincent's Hospital, University of Melbourne, Melbourne, Australia.
James D
JD
Best
Department of Medicine, St Vincent's Hospital, University of Melbourne, Melbourne, Australia.
David N
DN
O'Neal
NHMRC Clinical Trials Centre, University of Sydney, Camperdown, NSW, Australia Department of Medicine, St Vincent's Hospital, University of Melbourne, Melbourne, Australia.
Alicia J
AJ
Jenkins
Semicarbazide-sensitive amine oxidase; diabetes; inflammation; oxidative stress; renal dysfunction; type 1