The unsaponifiable fraction of extra virgin olive oil promotes apoptosis and attenuates activation and homing properties of T cells from patients with inflammatory bowel disease.
The unsaponifiable fraction (UF) of extra virgin olive oil (EVOO) possesses anti-inflammatory properties and exerts preventative effects in murine models of inflammatory bowel disease (IBD). The present study was designed to determine the in vitro effects of UF on blood and intestinal T cells from IBD patients and healthy subjects. The T cell phenotype was investigated by flow cytometry and cytokine secretion was determined by ELISA. The presence of UF of EVOO promoted apoptosis and attenuated activation of intestinal and blood T cells isolated from IBD patients, decreasing the frequency of CD69(+) and CD25(+) T cells and, also, the secretion of IFN-γ. Moreover, UF reduced the expression of the gut homing receptor integrin β7 on blood T cells from IBD patients. In conclusion, UF modulates the activity and the gut homing capacity of T cells, and might therefore be considered as a dietary complement with an anti-inflammatory role in IBD patients.
Dept. Microbiology and Immunology, Sahlgrenska Academy at the University of Gothenburg, Sweden; Dept. Pharmacology, School of Pharmacy, University of Seville, Spain.
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Cárdeno
Dept. Microbiology and Immunology, Sahlgrenska Academy at the University of Gothenburg, Sweden; Dept. Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Sweden.
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Magnusson
Dept. Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Sweden.
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Strid
Dept. Pharmacology, School of Pharmacy, University of Seville, Spain.
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Alarcón de La Lastra
Dept. Pharmacology, School of Pharmacy, University of Seville, Spain.
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Sánchez-Hidalgo
Dept. Microbiology and Immunology, Sahlgrenska Academy at the University of Gothenburg, Sweden; Dept. Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Sweden. Electronic address: lena.ohman@microbio.gu.se.
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Ohman
D000328:Adult; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000276:immunology*; D039641:Integrin beta Chains / Q000032:analysis; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008875:Middle Aged; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000502:physiology
D000893:Anti-Inflammatory Agents; D039641:Integrin beta Chains; D000069463:Olive Oil; D010938:Plant Oils; C091368:integrin beta7
Extra virgin olive oil; Inflammatory bowel disease; T cells; Unsaponifiable fraction