Detection of human Bocavirus 1, 2 and 3 from patients with acute gastroenteritis.
OBJECTIVE
We aimed to investigate the prevalence of Human Bocavirus isolates among Iranian patients suffering from acute gastroenteritis.


BACKGROUND
Human Bocavirus is a new parvovirus that has been identified in association with gastroenteritis. Limited data are available about HBoV in Iran.


METHODS
Viral DNA was extracted from all 294 stool samples. HBoV DNA was detected in extracted samples by polymerase chain reaction (PCR) amplification of a 354 bp of noncapsid protein 1 (NP1) gene. In addition, all samples were also subjected to a nested PCR to amplify a 455 bp of nonstructural 1 (NS1) gene.


RESULTS
The main clinical symptoms among HBoV positive patients were diarrhea (77.7%), fever (62.9%), vomiting (55.5%), and anorexia (59.2%). NP1 PCR was positive in 8 samples (2.72%), NS1 was positive in 16 patients (5.44%) and 3 samples had positive results in both regions (1.02%).


CONCLUSIONS
Our results suggest that HBoV could be considered as one of the important etiologic agents of acute gastroenteritis cases in Iran.
Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Sara
S
Romani
Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Seyed Reza
SR
Mohebbi
Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mahsa
M
Khanyaghma
Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Pedram
P
Azimzadeh
Basic and Molecular Epidemiology of Gastroenterology Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Sajad Majidizadeh
SM
Bozorgi
Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Behzad
B
Damavand
Department of Pathology and Laboratory sciences, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Farzaneh
F
Jadali
Acute gastroenteritis; Human Bocavirus; Iran