Polyclonal murine and rabbit antibodies for the bile acid isolithocholic acid.
Bile acids are relevant markers for clinical research. This study reports the production of antibodies for isolithocholic acid, the isomer of the extensively studied lithocholic acid. The IgG titer and affinity maturation were monitored during the immunizations of three mice and two rabbits. In both animal models, polyclonal antibodies with a high selectivity and affinity were produced. The development of a direct competitive ELISA with a test midpoint of 0.69 ± 0.05 μ g/L and a measurement range from 0.09-15 μg/L is reported. Additionally, the crystal structure of isolithocholic acid is described for the first time.
a BAM Federal Institute for Materials Research and Testing , Berlin , Germany.
Stefanie
S
Gärtner
José João
JJ
Carvalho
Franziska
F
Emmerling
Leif-Alexander
LA
Garbe
Rudolf J
RJ
Schneider
D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology; D008095:Lithocholic Acid / Q000032:analysis* / Q000276:immunology; D051379:Mice; D011817:Rabbits
D001647:Bile Acids and Salts; D007074:Immunoglobulin G; D008095:Lithocholic Acid
3β-hydroxy-5β-cholanic acid; ELISA; competitive assay; crystal structure; immunization monitoring