A sandwich substrate for ultrasensitive and label-free SERS spectroscopic detection of folic acid / methotrexate.
A highly sensitive surface enhanced Raman scattering (SERS) substrate with particle-film sandwich geometry has been developed for the label free detection of folic acid (FA) and methotrexate (MTX). In this sandwich structure, the bottom layer is composed of a copper foil decorated with silver nanoparticles synthesized by the galvanic displacement reaction, and top layer is constituted by silver nanoparticles. The FA and MTX molecules are sandwiched between the silver nanoparticles decorated copper film and the silver nanoparticles. The plasmonic coupling between the two layers of the sandwich structure greatly enhances the SERS spectra of FA and MTX. SERS activity of the substrate was studied and optimized by adjusting the time of galvanic displacement reaction. The SERS spectra of the FA and MTX showed the minimum detection concentration of 100 pM. The identification of methotrexate and folic acid analogs was also carried out by SERS spectra analysis.
Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI, USA.
Jing
J
Yang
Xuebin
X
Tan
Wei-Chuan
WC
Shih
Mark Ming-Cheng
MM
Cheng
D003300:Copper / Q000737:chemistry*; D005492:Folic Acid / Q000032:analysis*; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D008727:Methotrexate / Q000032:analysis*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods
D008567:Membranes, Artificial; D012834:Silver; D003300:Copper; D005492:Folic Acid; D008727:Methotrexate