Glucose-stimulated insulin secretion: A newer perspective.
Existing concepts and models for glucose-stimulated insulin secretion (GSIS) are overviewed and a newer perspective has been formulated toward the physiological understanding of GSIS. A conventional model has been created on the basis of in vitro data on application of a square wave high glucose in the absence of any other stimulatory inputs. Glucose elicits rapid insulin release through an adenosine triphosphate-sensitive K(+) channel (KATP channel)-dependent mechanism, which is gradually augmented in a KATP channel-independent manner. Biphasic GSIS thus occurs. In the body, the β-cells are constantly exposed to stimulatory signals, such as glucagon-like peptide 1 (GLP-1), parasympathetic inputs, free fatty acid (FFA), amino acids and slightly suprathreshold levels of glucose, even at fasting. GLP-1 increases cellular cyclic adenosine monophosphate, parasympathetic stimulation activates protein kinase C, and FFA, amino acids and glucose generate metabolic amplification factors. Plasma glucose concentration gradually rises postprandially under such tonic stimulation. We hypothesize that these stimulatory inputs together make the β-cells responsive to glucose independently from its action on KATP channels. Robust GSIS in patients with a loss of function mutation of the sulfonylurea receptor, a subunit of KATP channels, is compatible with this hypothesis. Furthermore, pre-exposure of the islets to an activator of protein kinase A and/or C makes β-cells responsive to glucose in a KATP channel- and Ca(2+)-independent manner. We hypothesize that GSIS occurs in islet β-cells without glucose regulation of KATP channels in vivo, for which priming with cyclic adenosine monophosphate, protein kinase C and non-glucose nutrients are required. To understand the physiology of GSIS, comprehensive integration of accumulated knowledge is required.
Department of Internal Medicine Division of Diabetes, Endocrinology and Metabolism Shinshu University School of Medicine Matsumoto Nagano Japan.
Mitsuhisa
M
Komatsu
Department of Internal Medicine Division of Diabetes, Endocrinology and Metabolism Shinshu University School of Medicine Matsumoto Nagano Japan.
Masahiro
M
Takei
Department of Internal Medicine Division of Diabetes, Endocrinology and Metabolism Shinshu University School of Medicine Matsumoto Nagano Japan.
Hiroaki
H
Ishii
Department of Internal Medicine Division of Diabetes, Endocrinology and Metabolism Shinshu University School of Medicine Matsumoto Nagano Japan.
Yoshihiko
Y
Sato
Adenosine triphosphate‐sensitive K+ channel; Modulatory signals; Physiological insulin secretion