Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles.
Dark field microscopy is a widely unknown method to measure the particle size distribution of diffusing nanoparticles by particle tracking. Here we demonstrate that by using the surface plasmonic resonance of Au nanoparticles, size differences of ca. 20 nm can be identified within the particle size distribution. For that purpose, we developed a software tool which helps to analyze color videos of diffusing nanoparticles retrieved from CCD or CMOS cameras. Polystyrene beads with a diameter of 100 and 200 nm were used to compare the results to those obtained with a well-established laser-based particle tracking system. The methodology will be discussed in the light of recent developments in the emerging field of optical nanoparticle tracking.
Biomedical Imaging Group, Dortmund University of Applied Sciences and Arts, Emil-Figge-Straße 42, 44227 Dortmund, Germany.
Thorsten
T
Wagner
Biomedical Imaging Group, Dortmund University of Applied Sciences and Arts, Emil-Figge-Straße 42, 44227 Dortmund, Germany.
Hans-Gerd
HG
Lipinski
IBE R&D Institute for Lung Health gGmbH, Mendelstraße 11, 48149 Münster, Germany.
Martin
M
Wiemann
Dark field microscopy ; ImageJ; Instrumentation development; Nanoparticle tracking analysis; Particle characterization; Surface plasmon resonance