Pre-existing hypertension dominates γδT cell reduction in human ischemic stroke.
T lymphocytes may play an important role in the evolution of ischemic stroke. Depletion of γδT cells has been found to abrogate ischemia reperfusion injury in murine stroke. However, the role of γδT cells in human ischemic stroke is unknown. We aimed to determine γδT cell counts and γδT cell interleukin 17A (IL-17A) production in the clinical setting of ischemic stroke. We also aimed to determine the associations of γδT cell counts with ischemic lesion volume, measures of clinical severity and with major stroke risk factors. Peripheral blood samples from 43 acute ischemic stroke patients and 26 control subjects matched on race and gender were used for flow cytometry and complete blood count analyses. Subsequently, cytokine levels and gene expression were measured in γδT cells. The number of circulating γδT cells was decreased by almost 50% (p = 0.005) in the stroke patients. γδT cell counts did not correlate with lesion volume on magnetic resonance diffusion-weighted imaging or with clinical severity in the stroke patients, but γδT cells showed elevated levels of IL-17A (p = 0.048). Decreased γδT cell counts were also associated with older age (p = 0.004), pre-existing hypertension (p = 0.0005) and prevalent coronary artery disease (p = 0.03), with pre-existing hypertension being the most significant predictor of γδT cell counts in a multivariable analysis. γδT cells in human ischemic stroke are reduced in number and show elevated levels of IL-17A. A major reduction in γδT lymphocytes also occurs in hypertension and may contribute to the development of hypertension-mediated stroke and vascular disease.
Department of Neurology, SUNY Downstate Medical Center, Brooklyn, New York, United States of America; Department of Neurology, Jagiellonian University Medical College, Krakow, Poland.
Mateusz G
MG
Adamski
Department of Neurology, SUNY Downstate Medical Center, Brooklyn, New York, United States of America.
Yan
Y
Li
Department of Neurology, SUNY Downstate Medical Center, Brooklyn, New York, United States of America.
Erin
E
Wagner
Department of Neurology, SUNY Downstate Medical Center, Brooklyn, New York, United States of America.
Hua
H
Yu
Department of Neurology, SUNY Downstate Medical Center, Brooklyn, New York, United States of America.
Chloe
C
Seales-Bailey
Department of Pathology, SUNY Downstate Medical Center, Brooklyn, New York, United States of America.
Helen
H
Durkin
Department of Neurology, SUNY Downstate Medical Center, Brooklyn, New York, United States of America.
Qing
Q
Hao
University of North Carolina, Chapel Hill, North Carolina, United States of America.
Steven A
SA
Soper
Louisiana State University, Baton Rouge, Louisiana, United States of America.
Michael
M
Murphy
Department of Neurology, SUNY Downstate Medical Center, Brooklyn, New York, United States of America.
Alison E
AE
Baird
D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D001772:Blood Cell Count; D016207:Cytokines / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006973:Hypertension / Q000150:complications / Q000276:immunology*; D020381:Interleukin-17 / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D018709:Statistics, Nonparametric; D020521:Stroke / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D016207:Cytokines; C508717:IL17A protein, human; D020381:Interleukin-17; D016692:Receptors, Antigen, T-Cell, gamma-delta