Polypeptide-based aerosol nanoparticles: self-assembly and control of conformation by solvent and thermal annealing.
Nanoconfined self-assemblies within aerosol nanoparticles and control of the secondary structures are shown here upon ionically complexing poly(L-lysine) (PLL) with dodecylbenzenesulfonic acid (DBSA) surfactant and using solvents chloroform, 1-propanol, or dimethylformamide. Different solvent volatilities and drying temperatures allowed tuning the kinetics of morphology formation. The supramolecular self-assembly and morphology were studied using cryo-TEM and SEM, and the secondary structures, using FT-IR. Highly volatile chloroform led to the major fraction of α-helical conformation of PLL(DBSA), whereas less volatile solvents or higher drying temperatures led to the increasing fraction of β-sheets. Added drugs budesonide and ketoprofen prevented β-sheet formation and studied PLL(DBSA)-drug nanoparticles were in the α-helical conformation. Preliminary studies showed that ketoprofen released with a slower rate than budesonide which was hypothesized to result from different localization of drugs within the PLL(DBSA) nanoparticles. These results instruct to prepare polypeptide aerosol nanoparticles with internal self-assembled structures and to control the secondary structures by aerosol solvent annealing, which we foresee to be useful, e.g., toward controlling the release of poorly soluble drug molecules.
Department of Applied Physics, Aalto University School of Science , P.O. Box 15100, FIN-00076 Aalto, Espoo, Finland.
Antti
A
Rahikkala
Susanna
S
Junnila
Ville
V
Vartiainen
Janne
J
Ruokolainen
Olli
O
Ikkala
Esko
E
Kauppinen
Janne
J
Raula
D000433:1-Propanol / Q000737:chemistry; D000336:Aerosols; D001557:Benzenesulfonates / Q000737:chemistry; D019819:Budesonide / Q000737:chemistry; D002725:Chloroform / Q000737:chemistry; D004126:Dimethylformamide / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D007660:Ketoprofen / Q000737:chemistry; D007700:Kinetics; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry; D017433:Protein Structure, Secondary; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
D000336:Aerosols; D001557:Benzenesulfonates; D004337:Drug Carriers; D010455:Peptides; D012997:Solvents; D013501:Surface-Active Agents; D011107:Polylysine; D019819:Budesonide; C001114:dodecylbenzenesulfonic acid; D002725:Chloroform; D004126:Dimethylformamide; D007660:Ketoprofen; D000433:1-Propanol