Electroacupuncture pretreatment inhibits NADPH oxidase-mediated oxidative stress in diabetic mice with cerebral ischemia.
We investigated the protective effect of electroacupuncture (EA) on cerebral ischemic injury in diabetic mice, and explored the role of NADPH oxidase-mediated oxidative stress. Male C57BL/6 mice were injected streptozotocin to induce diabetes. The mice were pretreated with EA at acupoint "Baihui" for 30 min. Two hours after the end of EA pretreatment, focal cerebral ischemia was induced following 24h reperfusion. The neurobehavioral scores and infarction volumes, malondialdehyde (MDA), reactive oxygen species (ROS), and activation of NADPH oxidase were determined in the presence or absence of the NADPH oxidase inhibitor apocynin or activator tetrabromocinnamic acid (TBCA). EA pretreatment reduced infarct size and improved neurological outcomes 24h after reperfusion in the diabetic mice. EA also decreased cerebral MDA and ROS levels compared with the control group, and inhibited the NADPH oxidase activation. The beneficial effects were abolished by TBCA while pretreatment with apocynin mimicked the neuroprotective and anti-oxidative effects of EA. Our results demonstrated that EA attenuated cerebral ischemic injury by inhibiting NAPDH oxidase-mediated oxidative damage in diabetic mice. These results suggest a novel mechanism of EA pretreatment-induced tolerance in diabetic cerebral ischemia.
Department of Anesthesiology, Xijing Hospital, Forth Military Medical University, Xi׳an, China; Department of Radiology, Xijing Hospital, Forth Military Medical University, Xi׳an, China.
Fan
F
Guo
Department of Anesthesiology, Xijing Hospital, Forth Military Medical University, Xi׳an, China.
Wenying
W
Song
Department of Anesthesiology, Xijing Hospital, Forth Military Medical University, Xi׳an, China.
Tao
T
Jiang
Department of Anesthesiology, School of Medicine, Stony Brook University, NY 11794-8480, USA.
Lixin
L
Liu
Department of Anesthesiology, Xijing Hospital, Forth Military Medical University, Xi׳an, China.
Feng
F
Wang
Department of Anesthesiology, Xijing Hospital, Forth Military Medical University, Xi׳an, China.
Haixing
H
Zhong
Department of Radiology, Xijing Hospital, Forth Military Medical University, Xi׳an, China.
Hong
H
Yin
Department of Anesthesiology, Xijing Hospital, Forth Military Medical University, Xi׳an, China. Electronic address: wangqiang@fmmu.edu.cn.
Qiang
Q
Wang
Department of Anesthesiology, Xijing Hospital, Forth Military Medical University, Xi׳an, China. Electronic address: mzkxlz@126.com.
Lize
L
Xiong
D000098:Acetophenones / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D002545:Brain Ischemia / Q000150:complications / Q000201:enzymology / Q000473:pathology / Q000628:therapy*; D002934:Cinnamates / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D015671:Electroacupuncture / Q000379:methods*; D020536:Enzyme Activators / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008810:Mice, Inbred C57BL; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000150:complications / Q000201:enzymology / Q000517:prevention & control
D000098:Acetophenones; D002934:Cinnamates; D020536:Enzyme Activators; D017382:Reactive Oxygen Species; C518786:tetrabromocinnamic acid; D008315:Malondialdehyde; C056165:acetovanillone; D019255:NADPH Oxidases
Cerebral ischemia-reperfusion injury; Diabetes mellitus; Electroacupuncture; NADPH oxidase; Oxidative stress