Determination of mushroom toxins ibotenic acid, muscimol and muscarine by capillary electrophoresis coupled with electrospray tandem mass spectrometry.
The CE-ESI-MS/MS method for the identification, separation and determination of mushroom toxins, namely ibotenic acid, muscimol and muscarine, was developed. It proved to be sensitive and thus useful for the real sample analysis with omitting the labor and time consuming pretreatment step. The CE-ESI-MS/MS method was applied on the spiked human urine. The analytical characteristics of the proposed method, such as limits of detection, linearity and repeatability of the peak area and the migration time, were evaluated. The RSD of the migration time and peak area were from 0.93% to 1.60% and from 2.96% to 3.42%, respectively. The obtained LOD values were at the nanomolar concentration level, therefore the developed method is sufficient for the determination and quantification of studied toxins in human urine after mushroom intoxication.
Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, Olomouc CZ-771 46, Czech Republic. Electronic address: pavlina.ginterova@upol.cz.
Pavlína
P
Ginterová
PR LAB a.s., Agel Laboratory, Revoluční 2214/35, Nový Jičín CZ-741 01, Czech Republic.
Barbora
B
Sokolová
Department of Forensic Medicine and Medical Law, Faculty Hospital, Hněvotínská 3, Olomouc CZ-771 46, Czech Republic.
Peter
P
Ondra
Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, Olomouc CZ-771 46, Czech Republic.
Joanna
J
Znaleziona
Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, Olomouc CZ-771 46, Czech Republic.
Jan
J
Petr
Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, Olomouc CZ-771 46, Czech Republic.
Juraj
J
Ševčík
Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, Olomouc CZ-771 46, Czech Republic.
Vítězslav
V
Maier
D000363:Agaricales / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D006801:Humans; D007051:Ibotenic Acid / Q000032:analysis* / Q000652:urine; D057230:Limit of Detection; D009116:Muscarine / Q000032:analysis* / Q000652:urine; D009118:Muscimol / Q000032:analysis* / Q000652:urine; D009145:Mushroom Poisoning / Q000652:urine*; D009995:Osmosis; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D016482:Urinalysis / Q000379:methods*
D007051:Ibotenic Acid; D009118:Muscimol; D009116:Muscarine
Amanita gemmata; Amanita muscaria; Amanita pantherina; Biological sample; Mushroom poisoning