Building proficient enzymes with foldamer prostheses.
Foldamers are non-natural oligomers that adopt stable conformations reminiscent of those found in proteins. To evaluate the potential of foldameric subunits for catalysis, semisynthetic enzymes containing foldamer fragments constructed from α- and β-amino acid residues were designed and characterized. Systematic variation of the α→β substitution pattern and types of β-residue afforded highly proficient hybrid catalysts, thus demonstrating the feasibility of expanding the enzyme-engineering toolkit with non-natural backbones.
Laboratory of Organic Chemistry, ETH Zürich, Hönggerberg HCI F339, 8093 Zürich (Switzerland).
Clemens
C
Mayer
Manuel M
MM
Müller
Samuel H
SH
Gellman
Donald
D
Hilvert
D002826:Chorismate Mutase / Q000737:chemistry / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D063747:Methanocaldococcus / Q000201:enzymology; D015202:Protein Engineering; D017433:Protein Structure, Secondary
D004798:Enzymes; D002826:Chorismate Mutase
enzymes; foldamers; protein design; protein engineering; β-amino acids