An extremely sensitive aptasensor based on interfacial energy transfer between QDS SAMs and GO.
In this work we designed a fluorescent self-assemblied multilayers, with thrombin aptamers and ssDNA as aptamer fixed onto the outermost layer, respectively. This multilayers can effectively sense biomolecules by interfacial florescence resonance energy transfer from multilayers to graphene oxide. High fluorescence quenching efficiency of graphene oxide and self-assemblied membrane' concentration results in good sensitivity for biosensing. A new interfacial sensing method with extremely high sensitivity for thrombin and DNA sequence was established, and the detection limit for thrombin and DNA was 16.2pM and 72.6 fM, respectively.
College of Materials Science and Engineering, Huaqiao University, 361021 Xiamen, China. Electronic address: sunxy@hqu.edu.cn.
Xiangying
X
Sun
College of Materials Science and Engineering, Huaqiao University, 361021 Xiamen, China.
Bin
B
Liu
College of Materials Science and Engineering, Huaqiao University, 361021 Xiamen, China.
Chuanxiao
C
Yang
College of Materials Science and Engineering, Huaqiao University, 361021 Xiamen, China.
Congcong
C
Li
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D013917:Thrombin / Q000032:analysis*
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D010087:Oxides; C081676:thrombin aptamer; D006108:Graphite; D004247:DNA; D013917:Thrombin
Aptasensors; CdTe; Fluorescence; Graphene oxide; Interfacial energy transfer; Self-assembled multilayers