Two-dimensional fluorescence-detected coherent spectroscopy with absolute phasing by confocal imaging of a dynamic grating and 27-step phase-cycling.
We present a novel experimental scheme for two-dimensional fluorescence-detected coherent spectroscopy (2D-FDCS) using a non-collinear beam geometry with the aid of "confocal imaging" of dynamic (population) grating and 27-step phase-cycling to extract the signal. This arrangement obviates the need for distinct experimental designs for previously developed transmission detected non-collinear two-dimensional coherent spectroscopy (2D-CS) and collinear 2D-FDCS. We also describe a novel method for absolute phasing of the 2D spectrum. We apply this method to record 2D spectra of a fluorescent dye in solution at room temperature and observe "spectral diffusion."
Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA.
Arijit K
AK
De
Department of Chemistry, University of California at Berkeley, Berkeley, California 94702, USA.
Daniele
D
Monahan
Department of Chemistry, University of California at Berkeley, Berkeley, California 94702, USA.
Jahan M
JM
Dawlaty
Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA.
Graham R
GR
Fleming
D002232:Carbocyanines / Q000032:analysis* / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007909:Lenses; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D057054:Molecular Imaging / Q000295:instrumentation* / Q000379:methods; D012031:Refractometry / Q000295:instrumentation* / Q000379:methods; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods
C529653:3,3'-diethyl-2,2'-thiatricarbocyanine; D002232:Carbocyanines