Longitudinal comparison of cytokines in peri-implant fluid and gingival crevicular fluid in healthy mouths.
BACKGROUND
Peri-implant and gingival tissues provide important sealing and protective functions around implants and teeth, but comparisons of the immunologic responses of these tissues after implant placement have not been conducted. Cytokine levels were measured in peri-implant crevicular fluid (PICF) and gingival crevicular fluid (GCF) as surrogate measures of immune function at subcrestally placed dental implants and healthy periodontal sites during a 1-year monitoring period.


METHODS
A total of 27 dental implants were placed subcrestally in 21 periodontally healthy patients (mean age: 49.0 ± 13.4 years). Repeated clinical and cytokine measurements were obtained over 12 months. GCF and PICF samples were collected and analyzed by cytokine microarray. Data were examined by non-parametric analysis of variance.


RESULTS
Plaque and bleeding indices were similar among all patients (P >0.05) at baseline. During 1 year of monitoring, the mean volumes of PICF and GCF were similar (P >0.05). The levels of interleukin (IL)-4, -6, -10, and -12p70, tumor necrosis factor-α, and interferon-γ in GCF and PICF were not significantly different and did not vary over time (P >0.05). The levels of IL-1α were higher in GCF than PICF at 1, 2, 6, and 12 months, as were the levels of IL-8 at 1, 2, 4, 6, and 12 months (P <0.001). Transforming growth factor-β1 in PICF and GCF exhibited time-dependent increases, and vascular endothelial growth factor was reduced at 1 year without differences between PICF and GCF (P >0.05).


CONCLUSIONS
Within the limitations of this study design, it can be concluded that after subcrestal implant placement, the immune response of peri-implant and periodontal tissues, as assessed by cytokine levels in PICF and GCF, is similar.
Faculty of Dentistry, University of Toronto, Toronto, ON.
Getulio
G
Nogueira-Filho
Igor
I
Pesun
Cindy
C
Isaak-Ploegman
Manjula
M
Wijegunasinghe
Tom
T
Wierzbicki
Christopher A
CA
McCulloch
D000328:Adult; D000368:Aged; D016207:Cytokines / Q000032:analysis*; D015921:Dental Implants; D003774:Dental Plaque Index; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D016753:Interleukin-10 / Q000032:analysis; D018664:Interleukin-12 / Q000032:analysis; D053582:Interleukin-1alpha / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D011446:Prospective Studies; D053773:Transforming Growth Factor beta1 / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
C508606:CXCL8 protein, human; D016207:Cytokines; D015921:Dental Implants; C508609:IL10 protein, human; C508598:IL4 protein, human; C508600:IL6 protein, human; D053582:Interleukin-1alpha; D015850:Interleukin-6; D016209:Interleukin-8; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma
Cytokines; dental implants; gingival crevicular fluid; inflammation; microarray analysis; wound healing