[Effect of hydrogen sulfide on human colon cancer SW480 cell proliferation and migration in vitro].
OBJECTIVE
To investigate the effect of hydrogen sulfide (H2S) on the proliferation and migration of human colon cancer SW480 cells and explore its molecular mechanisms.


METHODS
The proliferation of SW480 cells exposed to different concentrations of NaHS for varying time lengths was analyzed by MTT assay, and the changes in cell migration was evaluated using wound-healing assay. The changes in the expression levels of MMP-2, MMP-9 and SIRT1 protein were detected by Western blotting in the exposed cells.


RESULTS
Compared with the control cells, SW480 cells exposed to 50, 100, 200, or 400 µmol/L NaHS for 24, 48 and 72 h all showed increased proliferative activity. NaHS treatment at 100 µmol/L significantly promoted the cell migration (P<0.01) and enhanced the cellular expressions of MMP-2 (P<0.05) and MMP-9 (P<0.01) proteins; NaHS exposure (100 µmol/L) also resulted in up-regulation of SIRT1 expression in SW480 cells.


CONCLUSIONS
H2S can promote proliferation and migration of SW480 cells in vitro, the mechanism of which may involve up-regulated expression of SIRT1.
Southern Medical University, Guangzhou 510515, China; 2Department of Physiology, South China University, Hengyang 421001, China. E-mail: xiannv_2006@126.com.
Min
M
Hong
Xiaoqing
X
Tang
Kui
K
He
D045744:Cell Line, Tumor / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000473:pathology; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D013440:Sulfides / Q000494:pharmacology; D015854:Up-Regulation
D013440:Sulfides; C025451:sodium bisulfide; D006862:Hydrogen Sulfide