Antiadhesive polymer brush coating functionalized with antimicrobial and RGD peptides to reduce biofilm formation and enhance tissue integration.
This paper describes the synthesis and characterization of polymer-peptide conjugates to be used as infection-resistant coating for biomaterial implants and devices. Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP), able to kill bacteria on contact, and arginine-glycine-aspartate (RGD) peptides to promote the adhesion and spreading of host tissue cells. The antiadhesive and antibacterial properties of the coating were investigated with three bacterial strains: Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The ability of the coating to support mammalian cell growth was determined using human fibroblast cells. Coatings composed of the appropriate ratio of the functional components: PF127, PF127 modified with AMP, and PF127 modified with RGD showed good antiadhesive and bactericidal properties without hampering tissue compatibility.
University of Groningen and University Medical Center Groningen , Department of Biomedical Engineering, P.O. Box 196, 9700 AD Groningen, The Netherlands.
Agnieszka K
AK
Muszanska
Edward T J
ET
Rochford
Agnieszka
A
Gruszka
Andreas A
AA
Bastian
Henk J
HJ
Busscher
Willem
W
Norde
Henny C
HC
van der Mei
Andreas
A
Herrmann
D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects* / Q000235:genetics; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000502:physiology; D014018:Tissue Distribution / Q000187:drug effects / Q000502:physiology
D000890:Anti-Infective Agents; D009842:Oligopeptides; D011108:Polymers; C047981:arginyl-glycyl-aspartic acid