MicroRNA-199a-5p affects porcine preadipocyte proliferation and differentiation.
MicroRNAs (miRNAs), a class of small non-coding RNAs, have emerged as novel and potent regulators of adipogenesis. However, few miRNAs have been fully investigated in porcine adipogenesis, given the fact that pig is not only an apropos model of human obesity research, but also a staple meat source of human diet. In this study, we showed that miRNA-199a-5p is highly expressed in porcine subcutaneous fat deposits compared to several other tissue types and organs measured alongside. Overexpression of miR-199a-5p in porcine preadipocytes significantly promoted cell proliferation while attenuating the lipid deposition in porcine adipocytes. By target gene prediction and experimental validation, we demonstrated that caveolin-1 (Cav-1) may be a bona fide target of miR-199a-5p in porcine adipocytes, accounting for some of miR-199a-5p's functions. Taken together, our data established a role of miR-199a-5p in porcine preadipocyte proliferation and differentiation, which is at least partially played by downregulating Cav-1.
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. xineshi@nwsuaf.edu.cn.
Xin-E
XE
Shi
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. fengfeng0419@163.com.
Yue-Feng
YF
Li
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. xinongjialong@163.com.
Long
L
Jia
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. jihonglei2007@nwsuaf.edu.cn.
Hong-Lei
HL
Ji
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. songziyi89@gmail.com.
Zi-Yi
ZY
Song
Vitamin D Research Institute, Shaanxi University of Technology, Hanzhong 723000, China. bobos410@126.com.
Jia
J
Cheng
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. letitbe521@163.com.
Guo-Fang
GF
Wu
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. chengchuangsong@163.com.
Cheng-Chuang
CC
Song
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. langyan.95@163.com.
Qiang-Ling
QL
Zhang
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. jiayuzhulam@163.com.
Jia-Yu
JY
Zhu
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. gsyang999@hotmail.com.
Gong-She
GS
Yang
D020413:3' Untranslated Regions; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis; D000818:Animals; D001483:Base Sequence; D051242:Caveolin 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D013552:Swine
D020413:3' Untranslated Regions; D051242:Caveolin 1; D035683:MicroRNAs; D012333:RNA, Messenger