On the production of glycogen by Pseudomonas fluorescens during biofilm development: an in situ study by attenuated total reflection-infrared with chemometrics.
Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy was used to monitor Pseudomonas fluorescens biofilms in situ, non-destructively, in real time, and under fully hydrated conditions. Changes accompanying the metabolic evolution of the sessile bacterial cells from the nascent biofilm monolayer to the beginning of the multi-layered structure in the presence of nutrients were identified via the ATR-FTIR fingerprints of the young biofilm on the ATR crystal. The ATR-FTIR spectra were analysed by classical methods (time evolution of integrated intensities and profile evolution of specific bands), and also by a multivariate curve resolution, Bayesian positive source separation, to extract the pure component spectra and their change of concentration over time occurring during biofilm settlement. This work showed clearly the overproduction of glycogen by sessile P. fluorescens, which had not previously been described by other research groups.
a Université de Lorraine , Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, UMR 7564, F-54500 , Vandoeuvre-lès-Nancy , France.
Fabienne
F
Quilès
François
F
Humbert
D001499:Bayes Theorem; D018441:Biofilms / Q000254:growth & development*; D006003:Glycogen / Q000096:biosynthesis*; D008856:Microscopy, Fluorescence; D015999:Multivariate Analysis; D011551:Pseudomonas fluorescens / Q000254:growth & development / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D006003:Glycogen
ATR-FTIR spectroscopy; Pseudomonas fluorescens; bacterial biofilm; glycogen; multivariate curve resolution