Genetic heterogeneity of bovine noroviruses in Italy.
By screening 104 faecal samples from asymptomatic calves in Italy, bovine norovirus RNA was detected with a prevalence rate of 10.5 % (11/104). A continuous sequence spanning the RdRp region and the 5' end of the capsid gene was generated for 7 of the 11 strains. Upon phylogenetic analysis, five strains were grouped with GIII.2 Newbury2-like viruses, and one strain was grouped with GIII.1 Jena-like noroviruses. Interestingly, one strain (80TE/IT) was genetically related to the GIII.1/Jena/80/De in the RdRp but resembled the GIII.2/Newbury2/76/UK in the capsid gene, suggesting a recombination event occurring in the ORF1/ORF2 junction region.
Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro, 45, 64100, Teramo, Italy, bdimartino@unite.it.
Barbara
B
Di Martino
Federica
F
Di Profio
Elisabetta
E
Di Felice
Irene
I
Melegari
Chiara
C
Ceci
Axel
A
Mauroy
Etienne
E
Thiry
Vito
V
Martella
Fulvio
F
Marsilio
D000595:Amino Acid Sequence; D000818:Animals; D017250:Caliciviridae Infections / Q000453:epidemiology; D036022:Capsid Proteins / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology; D005243:Feces / Q000821:virology; D018740:Genetic Heterogeneity; D014644:Genetic Variation; D007558:Italy / Q000453:epidemiology; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames / Q000235:genetics*; D011995:Recombination, Genetic; D015678:Viral Structural Proteins / Q000235:genetics
D036022:Capsid Proteins; D015678:Viral Structural Proteins