Crystal structure of the transport unit of the autotransporter adhesin involved in diffuse adherence from Escherichia coli.
Several serious gastrointestinal diseases, which are widespread all over the world, are caused by enteropathogenic Escherichia coli. The monomeric autotransporter AIDA-I (adhesin involved in diffuse adherence) represents an important virulence factor of these strains and is involved in adhesion, biofilm formation, aggregation and invasion into host cells. Here, we present the crystal structure of the transport unit of AIDA-I at 3.0Å resolution, which forms a 12-stranded β-barrel harboring the linker domain in its pore. Mutagenesis studies of the C-terminal amino acid demonstrated the great impact of this terminal residue on membrane integration of AIDA-I and passenger translocation.
Institute of Biochemistry, Heinrich-Heine University Düsseldorf, 40225 Düsseldorf, Germany; Institute of Pharmaceutical and Medicinal Chemistry, PharmaCampus, Westphalian Wilhelms-University Münster, 48149 Münster, Germany.
Iris
I
Gawarzewski
Department of Biochemistry and HHMI, University of Washington, Seattle, WA 98195, USA.
Frank
F
DiMaio
Institute of Pharmaceutical and Medicinal Chemistry, PharmaCampus, Westphalian Wilhelms-University Münster, 48149 Münster, Germany.
Elisa
E
Winterer
Institute of Biochemistry, Heinrich-Heine University Düsseldorf, 40225 Düsseldorf, Germany.
Britta
B
Tschapek
Institute of Biochemistry, Heinrich-Heine University Düsseldorf, 40225 Düsseldorf, Germany.
Sander H J
SHJ
Smits
Institute of Pharmaceutical and Medicinal Chemistry, PharmaCampus, Westphalian Wilhelms-University Münster, 48149 Münster, Germany.
Joachim
J
Jose
Institute of Biochemistry, Heinrich-Heine University Düsseldorf, 40225 Düsseldorf, Germany. Electronic address: lutz.schmitt@hhu.de.
Lutz
L
Schmitt
D018830:Adhesins, Escherichia coli / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001692:Biological Transport; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics
C075559:AIDA-I protein, E coli; D018830:Adhesins, Escherichia coli; D011993:Recombinant Fusion Proteins
AIDA-I; Autotransporter; Escherichia coli; Membrane proteins; Outer membrane (OM); Protein secretion; Protein structure; Structural biology; Type V secretion; X-ray crystallography