Inhibitory effect of Raphanobrassica on Helicobacter pylori-induced gastritis in Mongolian gerbils.
Helicobacter pylori (H. pylori) infection is well known to be associated with chronic gastritis and also development of gastric cancer. Raphanobrassica (RB) is an intergeneric hybrid of the genera Raphanus (radish) and Brassica (cabbages) containing appreciable amounts of glucoraphanin (GR) and glucoraphenin (GRe), which are actively hydrolyzed by the enzyme myrosinase to sulforaphane and sulforaphene, respectively. Both of these metabolites exert antimicrobial and anti-inflammatory activity. The purpose of the present study was to investigate the effect of two freeze-dried products of RB (RB1 and RB2) on H. pylori-induced gastritis in Mongolian gerbils. Six-week-old male Mongolian gerbils were inoculated orally with H. pylori (ATCC 43504), and 2weeks later were fed diets containing no additives or diets supplemented with 2% RB1 (containing both GR and GRe) or 2% RB2 (containing GR only) for 10weeks. In the RB1, but not the RB2 group, mononuclear cell infiltration, mRNA expression of IL-6, and cell proliferation in the gastric mucosa were significantly suppressed. These results indicate that RB1 containing both GR and GRe exerted significant inhibitory effects on H. pylori-induced gastritis in Mongolian gerbils apparently mediated via suppression of IL-6 expression and chronic inflammation.
Department of Pathology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan.
Takanori
T
Yamada
Department of Pathology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan.
Min
M
Wei
Division of Pathology, National Institute of Health Sciences, Tokyo 158-8501, Japan.
Takeshi
T
Toyoda
Department of Pathology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan.
Shoutaro
S
Yamano
Department of Pathology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. Electronic address: wani@med.osaka-cu.ac.jp.
Hideki
H
Wanibuchi
D000818:Animals; D019607:Brassicaceae / Q000737:chemistry*; D002908:Chronic Disease; D004195:Disease Models, Animal; D005756:Gastritis / Q000188:drug therapy* / Q000382:microbiology; D005849:Gerbillinae; D005961:Glucosinolates / Q000494:pharmacology; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000187:drug effects*; D007096:Imidoesters / Q000494:pharmacology; D007249:Inflammation / Q000188:drug therapy; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008297:Male; D010091:Oximes; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013454:Sulfoxides
D005961:Glucosinolates; D007096:Imidoesters; D015850:Interleukin-6; D010091:Oximes; D010936:Plant Extracts; D012333:RNA, Messenger; D013454:Sulfoxides; C522767:glucoraphenin; C119494:glucoraphanin
Chronic gastritis; Gastric carcinogenesis; Helicobacter pylori; Inhibition of inflammation; Mongolian gerbils; Raphanobrassica