Yeast survival and growth assays.
Saccharomyces cerevisiae is an ideal model for lower eukaryotic microorganisms in the study of a variety of biochemical, genetic, and cellular processes. The quantitative or semiquantitative assessment of cell growth and survival under given conditions is a critical technique in the above studies. This chapter describes three complementary protocols to measure relative survival of yeast cells under specific experimental conditions. Similar methods can also be applied to other microorganisms and adapted to study cell growth in addition to survival.
College of Life Sciences, Capital Normal University, 105 3rd Ring Road West, Beijing, 100048, People's Republic of China.
Xin
X
Xu
Amanda D
AD
Lambrecht
Wei
W
Xiao
D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development