Preliminary phytochemical and biological evaluation of daphne glomerata extracts.
The article reports on the phytochemical and biological potentials of leaves and stems of Daphne glomerata Lam. (Fam. Thymelaeaceae). Study indicated that crude extracts from the leaves and stems of the plant have high biological activity. Cytotoxicity was assessed using resazurin on an automated 96-well Fluoroskan Ascent F1_ plate reader (Labsystems) using excitation and emission wavelengths of 530 nm and 590 nm, respectively. High cytotoxic activity (<1,563 µg/ml) of both extracts toward lung carcinoma cell lines was elucidated. The specificity of extracts to WS-1 human fibroblast cell lines was observed. For the first time, the profiles of compounds with terpenic and phenolic nature were described in Daphne glomerata leaves and stems MeOH extracts by TLC and (HPLC-DAD-MS) techniques.
Tbilisi State Medical University I.Kutateladze Institute of Pharmacochemistry, Georgia.
B
B
Tabidze
Tbilisi State Medical University I.Kutateladze Institute of Pharmacochemistry, Georgia.
G
G
Moshiashvili
Tbilisi State Medical University I.Kutateladze Institute of Pharmacochemistry, Georgia.
N
N
Tabatadze
Tbilisi State Medical University I.Kutateladze Institute of Pharmacochemistry, Georgia.
J
J
Aneli
Tbilisi State Medical University I.Kutateladze Institute of Pharmacochemistry, Georgia.
V
V
Mshvildadze
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D032364:Daphne / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts