Dopamine functionalized-CdTe quantum dots as fluorescence probes for l-histidine detection in biological fluids.
In this paper, we developed dopamine functionalized-CdTe quantum dots as fluorescence probes for the determination of l-histidine. Firstly, CdTe was covalently linked to dopamine to form a kind of fluorescence sensor with pyrocatechol structure on the surface. The photoluminescence intensity of CdTe-dopamine (QDs-DA) could be quenched by Ni(2+) due to the strong coordination interaction between the pyrocatechol structure of QDs-DA and Ni(2+). In the presence of l-histidine, Ni(2+) preferred to bind with l-histidine due to high affinity of Ni(2+) to l-histidine and the photoluminescence intensity of QDs-DA was recovered. The recovered photoluminescence intensity of QDs-DA was proportional to the concentration of l-histidine in the ranges of 1.0×10(-6)-1.0×10(-4)mol L(-1) and the detection limit was 5.0×10(-7)mol L(-1) respectively. The established method showed a good selectivity for l-histidine among other common amino acids, and it was applied for determination of l-histidine in human serum sample with satisfactory results.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Fanping
F
Shi
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Siyu
S
Liu
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: suxg@jlu.edu.cn.
Xingguang
X
Su
D000596:Amino Acids / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry; D019187:Cadmium Compounds / Q000737:chemistry*; D004298:Dopamine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D057230:Limit of Detection; D009532:Nickel / Q000737:chemistry; D010777:Photochemistry; D011485:Protein Binding; D045663:Quantum Dots; D012044:Regression Analysis; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013691:Tellurium / Q000737:chemistry*; D013696:Temperature
D000596:Amino Acids; D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D006639:Histidine; D009532:Nickel; D013691:Tellurium; C028337:cadmium telluride; D004298:Dopamine
Dopamine; Fluorescence; Ni(2+); Quantum dots; l-Histidine