Protein content analysis and antimicrobial activity of the crude venom of Montivipera bornmuelleri; a viper from Lebanon.
Viperidae snakes venoms represent a source of efficient bioactive components that have already led to the development of several new drugs. In this work, we analyzed the protein content of the Montivipera bornmuelleri crude venom using LC-ESI-MS, sephadex G-75 gel filtration and SDS-PAGE and demonstrated the presence of proteins with molecular masses corresponding to metalloprotease III, serine-protease and PLA2 in three fractions collected after gel filtration. Equally, we examined the antimicrobial effect of the venom that showed an important potency, as bactericidal agent, based on MBC and MIC values obtained, against Staphylococcus aureus and Morganella morganii bacteria. However, no activity was registered against Enterococcus faecalis, being the most resistant bacteria, neither against Aspergillus flavus and Penicillium digitatum fungal. Furthermore, on eleven other bacterial strains and the Candida albicans fungus, the venom has shown an intermediate efficacy by slightly reducing the growth. Our data concerning the Montivipera bornmuelleri venom give evidence of a rich and complex content aiding the exploration of new bioactive molecules for biopharmaceuticals purposes.
Claudine
C
Accary
Souad
S
Hraoui-Bloquet
Monzer
M
Hamze
Yassine
Y
Mallem
Fawaz
F
El Omar
Jean-Marc
JM
Sabatier
Jean-Claude
JC
Desfontis
Azm Center for Research in Biotechnology and its Applications, Doctoral School for Sciences and Technology or Faculty of Sciences III, Lebanese University, El Mittein Street, Tripoli, Lebanon. zfajloun@ul.edu.lb.
Ziad
Z
Fajloun
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D054501:Group II Phospholipases A2 / Q000302:isolation & purification; D007861:Lebanon; D019278:Matrix Metalloproteinase 3 / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D020613:Morganella morganii / Q000187:drug effects* / Q000254:growth & development; D040901:Proteomics / Q000379:methods; D030162:Reptilian Proteins / Q000302:isolation & purification; D057057:Serine Proteases / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D014757:Viper Venoms / Q000737:chemistry / Q000494:pharmacology*; D017819:Viperidae
D000900:Anti-Bacterial Agents; D030162:Reptilian Proteins; D014757:Viper Venoms; D054501:Group II Phospholipases A2; D057057:Serine Proteases; D019278:Matrix Metalloproteinase 3