Nicotine alters serum antioxidant profile in male albino rats.
BACKGROUND
Oxidative stress has repeatedly been implicated as the leading cause of several disease conditions.


OBJECTIVE
This study was designed to investigate the effects of nicotine administration on serum antioxidant levels in male albino rats.


METHODS
Forty male rats (150-180 g) were divided into five groups and treated orally for 30 days. Group I (control) received 0.2 ml/kg normal saline and Groups II and III received 0.5 and 1.0 mg/kg body weight (BW) of nicotine, respectively for 30 days. The fourth and fifth groups were administered with 0.5 and 1.0 mg/kg BW of nicotine for 30 days, but were left untreated for another 30 days. Serum was assayed for nitric oxide (NO), lipid peroxidation, and antioxidant enzyme.


RESULTS
The levels of superoxide dismutase (SOD) and catalase (CAT) were significantly decreased (P < 0.05) in 0.5 and 1.0 mg/kg nicotine treated groups. Glutathione peroxidase (GPx) and glutathione reductase (GR) were significantly decreased (P < 0.05), while NO and malondialdehyde (MDA) were significantly increased (P < 0.05) in 1.0 mg/kg treated group when compared with the control.


CONCLUSIONS
The present study shows that nicotine administration is associated with decreased serum antioxidant and increase lipid peroxidation ameliorated by nicotine withdrawal in male rat.
Department of Physiology, College of Health Sciences, Osun State University, Osogbo, Osun State, Nigeria ; Laboratory for Reproductive and Developmental Programming, Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Ibukun Peter
IP
Oyeyipo
Laboratory for Reproductive and Developmental Programming, Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Yinusa
Y
Raji
Laboratory for Reproductive and Developmental Programming, Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Adeyombo Folashade
AF
Bolarinwa
Antioxidant; Lipid peroxidation; Nicotine; Rats