Highly sensitive and selective determination of bisphenol-A using peptide-modified gold electrode.
Fast and accurate determination of bisphenol A (BPA) in varying matrices has become important in recent years. In this study, a cysteine-flanked heptapeptide sequence Cys-Lys-Ser-Leu-Glu-Asn-Ser-Tyr-Cys (CKSLENSYC), which is capable of recognizing BPA with high specificity, was isolated using a phage display technique. A novel electrochemical biosensor harnessing this affinity peptide as a BPA detection probe, was constructed and its performance was assessed. The formation of a self-assembled peptide monolayer on the gold electrode was confirmed by attenuated total reflection infrared spectroscopy (ATR-IR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Following the exploration of the optimum sensing condition, differential pulse voltammetry (DPV) was used to determine the varying concentrations of BPA in the solution. The developed sensor conveyed excellent performance in view of sensing speed, sensitivity and selectivity by detecting BPA in less than 5 min with a broad dynamic detection range of 1-5000 nM of BPA, despite the presence of several interfering species, such as phenolic compounds and inorganic ions.
School of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Jiao
J
Yang
School of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Sung-Eun
SE
Kim
School of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Misuk
M
Cho
School of Chemical Engineering and Bioengineering, University of Ulsan, Ulsan 680-749, Republic of Korea.
Ik-Keun
IK
Yoo
School of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea. Electronic address: checws@skku.edu.
Woo-Seok
WS
Choe
School of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea. Electronic address: yklee@skku.edu.
Youngkwan
Y
Lee
D000595:Amino Acid Sequence; D001559:Benzhydryl Compounds / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D004785:Environmental Pollutants / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D009842:Oligopeptides / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D006046:Gold; D009842:Oligopeptides; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Biosensor; Bisphenol A; Differential pulse voltammetry; Peptide; Self-assembled monolayer