A comparison of osteocyte bioactivity in fine particulate bone powder grafts vs larger bone grafts in a rat bone repair model.
The osteogenic potential for bone grafts is based on numbers and activities of cells that survive transplantation. In this study, we compared the bioactivity of osteocytes in 300-500 μm fine particulate bone powder grafts to 2 mm larger bone grafts in a rat radial defect model. Expression levels of bone morphogenetic protein-2 (BMP-2), transforming growth factor-beta 1 (TGF-β1), alkaline phosphatase (ALP), and collagen I were semi-quantified by both immunohistochemistry and RT-PCR at days 1 and 4, as well as weeks 1, 2, 4, 6 and 10 post-transplantation. Within two weeks post-transplantation, more cells stained positively for BMP-2, TGF-β1, ALP, and collagen I within the bone grafts and in the surrounding tissues in the group transplanted with the fine particulate bone powder grafts than in those with larger bone grafts (P<0.05). The mRNA levels of all four markers in the group transplanted with fine particulate bone powder graft peaked earlier and were expressed more highly than in the larger bone graft group, suggesting that fine particulate bone powder grafts provide more viable and active osteocytes to accelerate bone defect healing than larger bone grafts.
Department of Orthopaedic Surgery, Daqing Oil Field General Hospital, Daqing 163001, China.
Yuan-Xin
YX
Sun
Department of Orthopaedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Cheng-Li
CL
Sun
Department of Orthopaedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Ye
Y
Tian
Department of Orthopaedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Wen-Xiao
WX
Xu
Department of Orthopaedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Chang-Long
CL
Zhou
Department of Orthopaedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Chun-Yang
CY
Xi
Department of Orthopaedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Jing-Long
JL
Yan
Department of Orthopaedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address: xintaog6@163.com.
Xin-Tao
XT
Wang
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism; D001861:Bone Regeneration; D016025:Bone Transplantation; D001842:Bone and Bones / Q000502:physiology; D024042:Collagen Type I / Q000378:metabolism; D015870:Gene Expression; D008297:Male; D010011:Osteocytes / Q000502:physiology*; D011208:Powders; D011919:Rats, Inbred Strains; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D011208:Powders; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D000469:Alkaline Phosphatase
Bioactivity; Bone grafts; Bone powder; Osteocytes; Rat radial defect model