PHF8 and REST/NRSF co-occupy gene promoters to regulate proximal gene expression.
Chromatin regulators play an important role in the development of human diseases. In this study, we focused on Plant Homeo Domain Finger protein 8 (PHF8), a chromatin regulator that has attracted special concern recently. PHF8 is a histone lysine demethylase ubiquitously expressed in nuclei. Mutations of PHF8 are associated with X-linked mental retardation. It usually functions as a transcriptional co-activator by associating with H3K4me3 and RNA polymerase II. We found that PHF8 may associate with another regulator, REST/NRSF, predominately at promoter regions via studying several published PHF8 chromatin immunoprecipitation-sequencing (ChIP-Seq) datasets. Our analysis suggested that PHF8 not only activates but may also repress gene expression.
Department of Bioinformatics, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Juan
J
Wang
Department of Bioinformatics, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Xueqiu
X
Lin
Department of Bioinformatics, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Su
S
Wang
Department of Bioinformatics, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Chenfei
C
Wang
Department of Bioinformatics, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Qixuan
Q
Wang
Department of Bioinformatics, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Xikun
X
Duan
Fudan University, Shanghai Medical School, Department of Environmental Health.
Peng
P
Lu
Department of Bioinformatics, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Qian
Q
Wang
Department of Bioinformatics, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Chengyang
C
Wang
1] Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, MA 02215, USA [2] Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Harvard School of Public Health, Boston, MA 02215, USA.
X Shirley
XS
Liu
Department of Bioinformatics, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Jinyan
J
Huang
D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation; D056466:Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D059372:Nucleotide Motifs / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000099229:RE1-Silencing Transcription Factor
D002843:Chromatin; D056466:Histone Demethylases; D012319:RNA Polymerase II; D012097:Repressor Proteins; D014157:Transcription Factors; D000099229:RE1-Silencing Transcription Factor; C511934:PHF8 protein, human