Symmetrical and asymmetrical cyanine dyes. Synthesis, spectral properties, and BSA association study.
New cyanines were prepared by an efficient and practical route with satisfactory overall yield from low-cost starting materials. The photophysical behavior of the cyanines was investigated using UV-vis and steady-state fluorescence in solution, as well as their association with bovine serum albumin (BSA) in phosphate buffer solution (PBS). No cyanine aggregation was observed in organic solvents or in phosphate buffer solution. The alkyl chain length in the quaternized nitrogen was shown to be fundamental for BSA detection in PBS in these dyes.
Instituto de Quı́mica, Universidade Federal do Rio Grande do Sul , Avenida Bento Gonçalves, 9500, CP 15003. CEP 91501-970, Porto Alegre-RS, Brazil.
Diego S
DS
Pisoni
Letícia
L
Todeschini
Antonio César A
AC
Borges
Cesar L
CL
Petzhold
Fabiano S
FS
Rodembusch
Leandra F
LF
Campo
D002021:Buffers; D002232:Carbocyanines / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000138:chemical synthesis / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D014466:Ultraviolet Rays
D002021:Buffers; D002232:Carbocyanines; D004396:Coloring Agents; D005456:Fluorescent Dyes; D012996:Solutions; D012710:Serum Albumin, Bovine; D009584:Nitrogen