Histomorphometric analysis of early epithelialization and dermal changes in mid-partial-thickness burn wounds in humans treated with porcine small intestinal submucosa and silver-containing hydrofiber.
The objective of this study was to determine the healing rates of mid-partial-thickness burns treated with a porcine intestinal submucosa (SIS) vs. silver-containing cellulose hydrofiber (AgH) dressings. This was done by comparing healing response of burn wounds treated with SIS vs that of burns treated with AgH dressings. Five patients with mid-partial-thickness burns ≤10% of body surface were treated simultaneously, but in different areas, with SIS and AgH dressings; full-thickness biopsies were taken at days 0 and 7. Tissues treated with SIS presented higher epithelial maturation index (6.2 ± 0.84 vs. 3.2 ± 3.28; [mean ± standard deviation], P = .029), better orientation and differentiation of epithelial cells, as well as an appropriate basal lamina structure, collagen deposition, and higher transforming growth factor-β3 expression (7.4 ± 8.1 vs. 2.1 ± 2.6; P = .055) than tissues treated with AgH dressings. Importantly, after the treatment SIS was not integrated in healed tissues. After 3 months of treatment, SIS produced a lower score according to Vancouver Scar Scale (3.6 ± 2.6 vs. 7.2 ± 2.5, P = .025).The submucosa dressing does not simply act as scaffolding for the wound, it provides stimulation in the healing area, probably via growth factors initially present in SIS or matrikines derived from its digestion in the wound site. In conclusion, the present study demonstrated that biological matrices favor the wound-healing process.
From the *Laboratory of Connective Tissue, Centro Nacional de Investigación y Atención de Quemados, Instituto Nacional de Rehabilitación, Mexico City; †Burn Unit, Hospital Rubén Leñero, DDF, Mexico City; ‡Department of Pathology, Hospital Darío Fernández Fierro, ISSSTE, Mexico City; and §Department of Plastic Surgery, ISSEMYM, Mexico.
Rosa M
RM
Salgado
Leonardo
L
Bravo
Mario
M
García
Juan M
JM
Melchor
Edgar
E
Krötzsch
D000293:Adolescent; D000328:Adult; D000818:Animals; D001706:Biopsy; D002056:Burns / Q000188:drug therapy / Q000628:therapy*; D002266:Carboxymethylcellulose Sodium / Q000627:therapeutic use*; D003094:Collagen / Q000378:metabolism; D003646:Debridement; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa; D007421:Intestine, Small; D008297:Male; D009779:Occlusive Dressings; D011446:Prospective Studies; D013552:Swine; D053782:Transforming Growth Factor beta3 / Q000378:metabolism; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
D053782:Transforming Growth Factor beta3; D003094:Collagen; D002266:Carboxymethylcellulose Sodium