Localization of hepatocyte nuclear factor-4α in the nucleolus and nucleus is regulated by its C-terminus.
Aims/Introduction:  Mutations in hepatocyte nuclear factor-4α (HNF4α) lead to various diseases, among which C-terminal deletions of HNF4α are exclusively responsible for maturity onset diabetes of the young 1 (MODY1). MODY is an autosomal dominant disease characterized by a primary defect in insulin response to glucose, suggesting that the C-terminus of HNF4α is important for pancreatic β-cell function. To clarify the role of the C-terminus of HNF4α, changes in cellular localization and the binding ability to its regulator were examined, specifically in the region containing Q268, which deletion causes MODY1.


METHODS
Cellular localization of mutant HNF4α were examined in monkey kidney 7 (COS7), Chinese hamster ovary, rat insulinoma and mouse insulinoma cells, and their binding activity to other proteins were examined by fluorescence resonance energy transfer (FRET) in COS7 cells.


RESULTS
Although wild-type HNF4α was localized in the nucleoplasm in transfected cultured cells, Q268X-HNF4α was located predominantly in the nucleolus. Deletion analysis of the C-terminus of HNF4α showed that the S337X-HNF4α mutant, and other mutants with shorter amino acid sequences (S337-K194), were mostly localized in the nucleolus. HNF4α mutants with amino acid sequences shorter than the W192X-HNF4α mutant gradually spread to the nucleoplasm in accordance with their lengths. The A250X-HNF4α mutant was capable of causing the accumulation of HNF4α or the small heterodimer partner (SHP), one of the HNF4α regulators, in the nucleolus. However, the R154X-HNF4α mutant did not have binding ability to wild-type HNF4α or SHP, and thus was seen in the nucleus.


CONCLUSIONS
The C-terminus sites might play a key role in facilitating the nucleolar and subnucleolar localization of HNF4α. (J Diabetes Invest, doi: 10.1111/j.2040-1124.2012.00210.x, 2012).
Department of Medicine III and Diabetes Center, Tokyo Women's Medical University School of Medicine, Tokyo.
Makiko
M
Ogata
Department of Pharmacology, Saitama Medical University School of Medicine, Saitama, Japan.
Takeo
T
Awaji
Department of Medicine III and Diabetes Center, Tokyo Women's Medical University School of Medicine, Tokyo.
Naoko
N
Iwasaki
Department of Medicine III and Diabetes Center, Tokyo Women's Medical University School of Medicine, Tokyo.
Risa
R
Fujimaki
Department of Medicine III and Diabetes Center, Tokyo Women's Medical University School of Medicine, Tokyo.
Miho
M
Takizawa
Department of Pharmacology, Saitama Medical University School of Medicine, Saitama, Japan.
Kei
K
Maruyama
Department of Medicine and Human Genetics, University of Chicago, Chicago, IL, USA.
Graeme I
GI
Bell
Department of Medicine III and Diabetes Center, Tokyo Women's Medical University School of Medicine, Tokyo.
Yasuhiko
Y
Iwamoto
Department of Medicine III and Diabetes Center, Tokyo Women's Medical University School of Medicine, Tokyo.
Yasuko
Y
Uchigata
Fluorescence resonance energy transfer; Hepatocyte nuclear factor‐4α; Nucleolus