Molecular determinants of expansivity of native globular proteins: a pressure perturbation calorimetry study.
There is a growing interest in understanding how hydrostatic pressure (P) impacts the thermodynamic stability (ΔG) of globular proteins. The pressure dependence of stability is defined by the change in volume upon denaturation, ΔV = (∂ΔG/∂P)T. The temperature dependence of change in volume upon denaturation itself is defined by the changes in thermal expansivity (ΔE), ΔE = (∂ΔV/∂T)P. The pressure perturbation calorimetry (PPC) allows direct experimental measurement of the thermal expansion coefficient, α = E/V, of a protein in the native, αN(T), and unfolded, αU(T), states as a function of temperature. We have shown previously that αU(T) is a nonlinear function of temperature but can be predicted well from the amino acid sequence using α(T) values for individual amino acids (J. Phys. Chem. B 2010, 114, 16166-16170). In this work, we report PPC results on a diverse set of nine proteins and discuss molecular factors that can potentially influence the thermal expansion coefficient, αN(T), and the thermal expansivity, EN(T), of proteins in the native state. Direct experimental measurements by PPC show that αN(T) and EN(T) functions vary significantly for different proteins. Using comparative analysis and site-directed mutagenesis, we have eliminated the role of various structural or thermodynamic properties of these proteins such as the number of amino acid residues, secondary structure content, packing density, electrostriction, dynamics, or thermostability. We have also shown that αN(T) and EN,sp(T) functions for a given protein are rather insensitive to the small changes in the amino acid sequence, suggesting that αN(T) and EN(T) functions might be defined by a topology of a given protein fold. This conclusion is supported by the similarity of αN(T) and EN(T) functions for six resurrected ancestral thioredoxins that vary in sequence but have very similar tertiary structure.
Center for Biotechnology and Interdisciplinary Studies, ‡Departments of Biological Sciences, §Chemistry and Chemical Biology, and ∥Chemical and Biological Engineering, Rensselaer Polytechnic Institute , 110 8th Street, Troy, New York 12180, United States.
Daniel
D
Vasilchuk
Pranav P
PP
Pandharipande
Saba
S
Suladze
Jose M
JM
Sanchez-Ruiz
George I
GI
Makhatadze
D000595:Amino Acid Sequence; D000818:Animals; D002151:Calorimetry / Q000379:methods; D002417:Cattle; D002645:Chickens; D006736:Horses; D006801:Humans; D006874:Hydrostatic Pressure; D008969:Molecular Sequence Data; D009154:Mutation; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D019696:Peptidylprolyl Isomerase / Q000737:chemistry* / Q000235:genetics; D011312:Pressure; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D013696:Temperature; D013816:Thermodynamics
D000072340:NIMA-Interacting Peptidylprolyl Isomerase; C000606846:PIN1 protein, human; D019696:Peptidylprolyl Isomerase