Adiposed-derived stem cells seeded on PLCL/P123 eletrospun nanofibrous scaffold enhance wound healing.
The aim of this study was to evaluate the wound healing of adipose-derived stem cells (ADSCs) seeded on electrospinning poly (L-lactide-co-ε-caprolactone)/poloxamer (PLCL/P123) scaffolds and the mechanisms using a rat skin tissue injury model. CM-Dil labeled ADSCs were seeded on PLCL/P123 scaffolds for 48 h (ADSCs- PLCL/P123). Twelve Sprague-Dawley rats were randomly divided into three groups: ADSCs-PLCL/P123 (A group); PLCL/P123 alone (B group); petrolatum gauze (C group). Two 1.5 cm diameter circular impressions were made by placement of a punch biopsy instrument. The wound closure percentages were calculated by planimetry on postoperative day 0, 3, 12 and 21. On postoperative day 21, full-thickness skins from each group were examined by H&E staining, CK10 and CD31 immunohistochemical staining, and CK10 immunofluorescence staining respectively. The wound closure percentages on postoperative day 21 in A, B and C groups were 95.7% ± 3.9%, 83.2% ± 15.4% and 64.3% ± 6.2%, respectively (p < 0.05). H&E staining and immunohistochemical staining showed that the epidermal structure of A group was complete and possessed the characteristics of epidermal cells, and the CK10 staining positive suggested that ADSCs were differentiated into epidermal-like structures. The microvessel density of A group was significantly higher than that of the B and C groups (p < 0.05), while no significant differences were observed between B group and C group (p > 0.05). The ADSCs seeded on PLCL/P123 scaffolds could promote wound healing and have the potential to become a new therapeutic alternative material for skin tissue engineering.
Department of Plastic and Reconstructive Surgery, Zhongshan Hospital, Shanghai, 200032, People's Republic of China.
Jianying
J
Gu
Ninghua
N
Liu
Xinrong
X
Yang
Zihao
Z
Feng
Fazhi
F
Qi
D017667:Adipocytes / Q000166:cytology; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D003288:Contusions / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D004595:Electroplating / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D057139:Nanofibers / Q000737:chemistry* / Q000627:therapeutic use; D011060:Poloxalene / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012399:Rotation; D017768:Skin, Artificial; D033581:Stem Cell Transplantation / Q000295:instrumentation*; D013234:Stem Cells / Q000166:cytology; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000502:physiology*
D001672:Biocompatible Materials; D011091:Polyesters; C464484:pluronic block copolymer P123; C062968:lactide-caprolactone copolymer; D011060:Poloxalene