In vitro Therapeutic Effects of Low Level Laser at mRNA Level on the Release of Skin Growth Factors from Fibroblasts in Diabetic Mice.
BACKGROUND
Numerous in vitro reports suggest that Low Level Laser Therapy (LLLT) affects cellular processes by biostimulation, however most of them emphasize on using visible light lasers which have low penetration. The aim of this study was to determine the effect of infrared laser light (which is more useful in clinic because of its higher penetration) on secretion of Fibroblast Growth Factor (FGF), Platelet Derived Growth Factor (PDGF) and Vascular Endothelial Growth Factor (VEGF), as important growth factors in wound healing.


METHODS
Fibroblasts were extracted from the skin of 7 diabetic and 7 nondiabetic mice and cultured. Cell cultures of experimental group were irradiated with single dose of LLLT (energy density of 1 J/cm (2)) using an 810 nm continuous wave laser and the control group was not irradiated. Secretion of growth factors by skin fibroblasts were quantified through real time poly-merase chain reaction.


RESULTS
Diabetic irradiated group showed significant increase in FGF (p = 0.017) expression, although PDGF increased and VEGF decreased in both diabetic and nondiabetic irradiated groups, but these variations were not statistically significant.


CONCLUSIONS
These results suggest that LLLT may play an important role in wound healing by stimulating the fibroblasts.
Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
Nooshafarin Kazemi
NK
Khoo
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Mohammad Ali
MA
Shokrgozar
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Iraj Ragerdi
IR
Kashani
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Amir
A
Amanzadeh
Department of Epidemiology, Pasteur Institute of Iran, Tehran, Iran.
Ehsan
E
Mostafavi
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Hassan
H
Sanati
Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
Laleh
L
Habibi
Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
Saeid
S
Talebi
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Morteza
M
Abouzaripour
Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
Seyed Mohammad
SM
Akrami
Biostimulation; Cell culture; Low level laser therapy; Techniques; Wound healing