Quest for the binding mode of tetrabromobisphenol A with Calf thymus DNA.
The binding interaction of tetrabromobisphenol A with Calf thymus DNA was studied by multi-spectroscopic and molecular modeling methods. The UV-vis study revealed that an obvious interaction between tetrabromobisphenol A and Calf thymus DNA happened. The π-π(∗) transitions and the electron cloud of tetrabromobisphenol A might be changed by entering the groove of Calf thymus DNA. From the fluorescence spectral and thermodynamics studies, it was concluded that the hydrogen bonds and hydrophobic force played a major role in the binding of tetrabromobisphenol A to Calf thymus DNA. The molecular modeling study showed that the possible sites of tetrabromobisphenol A in the groove of DNA. Circular dichroism study also depicted that tetrabromobisphenol A bond to DNA. These above results would further advance our knowledge on the molecular mechanism of the binding interactions of brominated flame-retardants with nucleic acid.
Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China. Electronic address: wyqing76@126.com.
Yan-Qing
YQ
Wang
Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China.
Hong-Mei
HM
Zhang
Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China.
Jian
J
Cao
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005411:Flame Retardants / Q000032:analysis / Q000378:metabolism*; D006860:Hydrogen Bonding; D062105:Molecular Docking Simulation; D009690:Nucleic Acid Conformation; D011075:Polybrominated Biphenyls / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D005411:Flame Retardants; D011075:Polybrominated Biphenyls; D004247:DNA; C427203:calf thymus DNA; C020806:tetrabromobisphenol A
Binding mode; Calf thymus DNA; Molecular modeling; Spectroscopy; Tetrabromobisphenol A