CopA3 peptide prevents ultraviolet-induced inhibition of type-I procollagen and induction of matrix metalloproteinase-1 in human skin fibroblasts.
Ultraviolet (UV) exposure is well-known to induce premature aging, which is mediated by matrix metalloproteinase-1 (MMP-1) activity. A 9-mer peptide, CopA3 (CopA3) was synthesized from a natural peptide, coprisin, which is isolated from the dung beetle Copris tripartitus. As part of our continuing search for novel bioactive natural products, CopA3 was investigated for its in vitro anti-skin photoaging activity. UV-induced inhibition of type-I procollagen and induction of MMP-1 were partially prevented in human skin fibroblasts by CopA3 peptide in a dose-dependent manner. At a concentration of 25 μM, CopA3 nearly completely inhibited MMP-1 expression. These results suggest that CopA3, an insect peptide, is a potential candidate for the prevention and treatment of skin aging.
Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Korea.
Dong-Hee
DH
Kim
Advanced Medical Fusion Textile Center, Gyeongbuk Technopark Foundation, Gyeongsan 712-210, Korea.
Han-Hyuk
HH
Kim
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea.
Hyeon-Jeong
HJ
Kim
Radiation Division for Biotechnology, Advanced Radiation Technology Institute, Jeongeup Campus of Atomic Energy Research Institute (KAERI), Jeonbuk 580-185, Korea.
Hyun-Gug
HG
Jung
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea.
Jae-Myo
JM
Yu
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea.
Eun-Su
ES
Lee
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea.
Yong-Hun
YH
Cho
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea.
Dong-In
DI
Kim
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea. anbj@dhu.ac.kr.
Bong-Jeun
BJ
An
D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000037:antagonists & inhibitors* / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects*; D019476:Insect Proteins / Q000494:pharmacology*; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects; D014466:Ultraviolet Rays
D023181:Antimicrobial Cationic Peptides; D024042:Collagen Type I; C583724:CopA3 peptide, Copris tripartitus; D019476:Insect Proteins; D020781:Matrix Metalloproteinase 1