Endothelial impairment and bone marrow-derived CD34(+)/133(+) cells in diabetic patients with erectile dysfunction.
OBJECTIVE
The present study was undertaken to determine vascular endothelial impairment and endothelial progenitor cells (EPCs) in patients with type 2 diabetes mellitus and erectile dysfunction (ED).


METHODS
A total of 100 type 2 diabetic men were enrolled. Flow-mediated dilatation (FMD) and anaerobic threshold (AT) were measured. Also, EPCs in the peripheral blood were determined by flow cytometry.


RESULTS
In the 42 ED diabetic patients, FMD and AT were significantly less than those in the 58 patients with normal erectile function (FMD 2.84 vs 3.82%, P = 0.038, and AT 11.2 vs 12.7 mL/kg/min, P = 0.022). Exercise tolerance significantly increased the number of EPCs in the patients with and without ED (49-60 cells/100 μL, P = 0.015, and 72-99 cells/100 μL, P = 0.003). In the diabetic patients without autonomic neuropathy, FMD was significantly reduced in the patients with ED than those without ED (P = 0.015). In response to exercise tolerance, the number of EPCs increased in both the diabetic patients with ED (P = 0.003) and without ED (P = 0.007). In contrast, in the diabetic patients with autonomic neuropathy, there was no difference in FMD between the patients with and without ED. The exercise tolerance increased the number of EPCs in the patients without ED (P = 0.023), but it disappeared in those with ED.


CONCLUSIONS
ED diabetic patients have endothelial impairment during the early period of diabetic complications, whose deranged endothelial function is concomitantly repaired by promoting bone marrow-derived EPCs.
Department of Medicine Jichi Medical University Saitama Medical Center Saitama Japan.
Miho
M
Murata
Department of Biochemistry Jichi Medical University Shimotsuke Tochigi Japan.
Hiroyuki
H
Tamemoto
Department of Medicine Jichi Medical University Saitama Medical Center Saitama Japan.
Taeko
T
Otani
Department of Medicine Jichi Medical University Saitama Medical Center Saitama Japan.
Sachimi
S
Jinbo
Department of Medicine Jichi Medical University Saitama Medical Center Saitama Japan.
Nahoko
N
Ikeda
Department of Medicine Jichi Medical University Saitama Medical Center Saitama Japan.
Masanobu
M
Kawakami
Department of Medicine Jichi Medical University Saitama Medical Center Saitama Japan.
San-E
SE
Ishikawa
Diabetes mellitus; Endothelial progenitor cells; Erectile dysfunction