The mitochondrial genome of the leaf-cutter ant Atta laevigata: a mitogenome with a large number of intergenic spacers.
In this paper we describe the nearly complete mitochondrial genome of the leaf-cutter ant Atta laevigata, assembled using transcriptomic libraries from Sanger and Illumina next generation sequencing (NGS), and PCR products. This mitogenome was found to be very large (18,729 bp), given the presence of 30 non-coding intergenic spacers (IGS) spanning 3,808 bp. A portion of the putative control region remained unsequenced. The gene content and organization correspond to that inferred for the ancestral pancrustacea, except for two tRNA gene rearrangements that have been described previously in other ants. The IGS were highly variable in length and dispersed through the mitogenome. This pattern was also found for the other hymenopterans in particular for the monophyletic Apocrita. These spacers with unknown function may be valuable for characterizing genome evolution and distinguishing closely related species and individuals. NGS provided better coverage than Sanger sequencing, especially for tRNA and ribosomal subunit genes, thus facilitating efforts to fill in sequence gaps. The results obtained showed that data from transcriptomic libraries contain valuable information for assembling mitogenomes. The present data also provide a source of molecular markers that will be very important for improving our understanding of genomic evolutionary processes and phylogenetic relationships among hymenopterans.
Centro de Estudos de Insetos Sociais, UNESP - Univ Estadual Paulista. Rio Claro, São Paulo, Brazil; Instituto Oswaldo Cruz, FIOCRUZ - Fundação Oswaldo Cruz. Rio de Janeiro, Rio de Janeiro, Brazil.
Cynara de Melo
Cde M
Rodovalho
Departamento de Zoologia, UNESP - Univ Estadual Paulista. Rio Claro, São Paulo, Brazil.
Mariana Lúcio
ML
Lyra
Centro de Estudos de Insetos Sociais, UNESP - Univ Estadual Paulista. Rio Claro, São Paulo, Brazil.
Milene
M
Ferro
Centro de Estudos de Insetos Sociais, UNESP - Univ Estadual Paulista. Rio Claro, São Paulo, Brazil; Departamento de Bioquímica e Microbiologia, UNESP - Univ Estadual Paulista. Rio Claro, São Paulo, Brazil.
Maurício
M
Bacci
D000818:Animals; D001000:Ants / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics*; D019143:Evolution, Molecular; D015321:Gene Rearrangement / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D023281:Genomics / Q000379:methods; D010802:Phylogeny; D012343:RNA, Transfer / Q000235:genetics; D012270:Ribosomes / Q000235:genetics; D059467:Transcriptome / Q000235:genetics
D021901:DNA, Intergenic; D012343:RNA, Transfer