Nine human sparganosis cases in Thailand with molecular identification of causative parasite species.
Human sparganosis is one of the neglected diseases but important food-borne parasitic zoonoses. The disease is caused by larvae (spargana) of diphyllobothriidean tapeworm. Here, we describe nine cases of human sparganosis, caused by Spirometra erinaceieuropaei in a hospital in Thailand during 2001-2012. Clinical characteristics, treatment, and outcome of cases were revealed. Diagnosis and identification of causative parasite species was made by histopathological investigations followed by a polymerase chain reaction-based molecular method using formalin-fixed paraffin embedded tissues. The DNA samples were extracted from tissues and a partial fragment of cytochrome c oxidase subunit 1 (cox1) gene was amplified for the detection of parasitic DNA. Infection could be prevented by increasing activities on health communication by responsible public health agencies.
Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand; National Institute of Infectious Diseases, Tokyo 162-8640, Japan; Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Adhiratha
A
Boonyasiri
Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand; National Institute of Infectious Diseases, Tokyo 162-8640, Japan; Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Pornsuk
P
Cheunsuchon
Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand; National Institute of Infectious Diseases, Tokyo 162-8640, Japan; Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Yupin
Y
Suputtamongkol
Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand; National Institute of Infectious Diseases, Tokyo 162-8640, Japan; Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Hiroshi
H
Yamasaki
Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand; National Institute of Infectious Diseases, Tokyo 162-8640, Japan; Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Oranuch
O
Sanpool
Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand; National Institute of Infectious Diseases, Tokyo 162-8640, Japan; Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Wanchai
W
Maleewong
Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand; National Institute of Infectious Diseases, Tokyo 162-8640, Japan; Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand pewpan@kku.ac.th.
Pewpan M
PM
Intapan
D000328:Adult; D000368:Aged; D000818:Animals; D018509:DNA, Helminth / Q000145:classification / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000145:classification / Q000235:genetics*; D005260:Female; D005557:Formaldehyde; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D058069:Neglected Diseases; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013031:Sparganosis / Q000453:epidemiology* / Q000469:parasitology / Q000473:pathology / Q000601:surgery; D013032:Sparganum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D013785:Thailand / Q000453:epidemiology; D016610:Tissue Embedding
D018509:DNA, Helminth; D005557:Formaldehyde; D003576:Electron Transport Complex IV