Combined cross-linked enzyme aggregates (combi-CLEAs) for efficient integration of a ketoreductase and a cofactor regeneration system.
An alternative strategy for cofactor regeneration in the synthesis of valuable chiral alcohols catalyzed by ketoreductases was developed. combi-CLEAs of ketoreductase and d-glucose dehydrogenase enabled the repeated and effective conversion of substrate ethyl 4-chloro-3-oxobutanoate (COBE) with several superiorities. Wide application of this strategy in production of various chiral alcohols could be expected in the future for its high efficiency with low cost.
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China.
Chenxi
C
Ning
Enzyme and Fermentation Technology Laboratory, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, PR China. Electronic address: ezhsu@njfu.edu.cn.
Erzheng
E
Su
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China.
Yanjun
Y
Tian
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: dzhwei@ecust.edu.cn.
Dongzhi
D
Wei
D000090:Acetoacetates / Q000737:chemistry; D000438:Alcohols / Q000138:chemical synthesis / Q000737:chemistry*; D002384:Catalysis; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D010088:Oxidoreductases / Q000737:chemistry*; D013696:Temperature
D000090:Acetoacetates; D000438:Alcohols; D003432:Cross-Linking Reagents; C111982:ethyl 4-chloro-3-oxobutanoate; D010088:Oxidoreductases; D005947:Glucose
Asymmetric catalysis; Cofactor regeneration; Ketoreductase; combi-CLEAs