Gramella oceani sp. nov., a zeaxanthin-producing bacterium of the family Flavobacteriaceae isolated from marine sediment.
A Gram-staining-negative, yellow-pigmented, strictly aerobic, zeaxanthin-producing, rod-shaped, non-endospore-forming, appendaged bacterial strain that exhibits gliding motility, designated CC-AMSZ-T(T), was isolated from marine sediment off coastal Kending, Taiwan. Strain CC-AMSZ-T(T) shared 94.9% and 96.7-94.1% 16S rRNA gene sequence similarities with Gramella echinicola KMM 6050(T) and other species of the genus Gramella, respectively, and formed a distinct phyletic lineage in phylogenetic trees. The major (≥5% of the total) fatty acids were C(16 : 0), iso-C(15 : 0), anteiso-C(15 : 0), C(16 : 1)ω6c and/or C(16 : 1)ω7c and iso-C(17 : 1)ω9c and/or C(16 : 0) 10-methyl. Phosphatidylethanolamine, six unidentified lipids and three unidentified aminolipids were the polar lipid components. The DNA G+C content was 38.6 mol%. The predominant respiratory quinone was menaquinone-6 (MK-6). Based on the phylogenetic distinctiveness and distinguishing phenotypic characteristics, strain CC-AMSZ-T(T) represents a novel species of the genus Gramella, for which the name Gramella oceani sp. nov. is proposed. The type strain is CC-AMSZ-T(T) ( = JCM 18809(T) = BCRC 80547(T)).
Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Asif
A
Hameed
Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Mariyam
M
Shahina
Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Shih-Yao
SY
Lin
Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan, ROC.
You-Cheng
YC
Liu
Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Wei-An
WA
Lai
Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan, ROC.|Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Chiu-Chung
CC
Young
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013624:Taiwan; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D024341:Xanthophylls / Q000096:biosynthesis*; D065146:Zeaxanthins
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024341:Xanthophylls; D065146:Zeaxanthins; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6