Raman spectroscopic study of radioresistant oral cancer sublines established by fractionated ionizing radiation.
Radiotherapy is an important treatment modality for oral cancer. However, development of radioresistance is a major hurdle in the efficacy of radiotherapy in oral cancer patients. Identifying predictors of radioresistance is a challenging task and has met with little success. The aim of the present study was to explore the differential spectral profiles of the established radioresistant sublines and parental oral cancer cell lines by Raman spectroscopy. We have established radioresistant sublines namely, 50Gy-UPCI:SCC029B and 70Gy-UPCI:SCC029B from its parental UPCI:SCC029B cell line, by using clinically admissible 2Gy fractionated ionizing radiation (FIR). The developed radioresistant character was validated by clonogenic cell survival assay and known radioresistance-related protein markers like Mcl-1, Bcl-2, Cox-2 and Survivin. Altered cellular morphology with significant increase (p<0.001) in the number of filopodia in radioresistant cells with respect to parental cells was observed. The Raman spectra of parental UPCI:SCC029B, 50Gy-UPCI:SCC029B and 70Gy-UPCI:SCC029B cells were acquired and spectral features indicate possible differences in biomolecules like proteins, lipids and nucleic acids. Principal component analysis (PCA) provided three clusters corresponding to radioresistant 50Gy, 70Gy-UPCI:SCC029B sublines and parental UPCI:SCC029B cell line with minor overlap, which suggest altered molecular profile acquired by the radioresistant cells due to multiple doses of irradiation. The findings of this study support the potential of Raman spectroscopy in prediction of radioresistance and possibly contribute to better prognosis of oral cancer.
KS-121, Teni Laboratory, ACTREC, Tata Memorial Centre, Kharghar-Node, Navi Mumbai, India.
Mohd
M
Yasser
KS-04, Chilakapati Laboratory, ACTREC, Tata Memorial Centre, Kharghar-Node, Navi Mumbai, India.
Rubina
R
Shaikh
KS-04, Chilakapati Laboratory, ACTREC, Tata Memorial Centre, Kharghar-Node, Navi Mumbai, India.
Murali Krishna
MK
Chilakapati
KS-121, Teni Laboratory, ACTREC, Tata Memorial Centre, Kharghar-Node, Navi Mumbai, India.
Tanuja
T
Teni
D000704:Analysis of Variance; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D006801:Humans; D009062:Mouth Neoplasms / Q000000981:diagnostic imaging*; D025341:Principal Component Analysis; D011836:Radiation Tolerance; D011839:Radiation, Ionizing; D011859:Radiography; D013059:Spectrum Analysis, Raman / Q000379:methods*