Methods for multiplex template sampling in digital PCR assays.
The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. To solve this problem we present a strategy, called Multiplex Template Sampling (MTS), that effectively increases template concentrations by detecting multiple regions of fragmented target molecules. Three alternative assay approaches are presented for implementing MTS analysis of chromosome 21, providing a 10-fold concentration enhancement while preserving assay precision.
Centre for High-Throughput Biology, University of British Columbia, Vancouver, British Columbia, Canada.
Oleh I
OI
Petriv
Centre for High-Throughput Biology, University of British Columbia, Vancouver, British Columbia, Canada.
Kevin A
KA
Heyries
Centre for High-Throughput Biology, University of British Columbia, Vancouver, British Columbia, Canada.
Michael
M
VanInsberghe
Centre for High-Throughput Biology, University of British Columbia, Vancouver, British Columbia, Canada.
David
D
Walker
Centre for High-Throughput Biology, University of British Columbia, Vancouver, British Columbia, Canada; Department of Physics and Astronomy, University of British Columbia, Vancouver, British Columbia, Canada.
Carl L
CL
Hansen
D001483:Base Sequence; D002891:Chromosomes, Human, Pair 21; D017931:DNA Primers; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers