Evaluation of periodontal pathogens of the mandibular third molar pericoronitis by using real time PCR.
OBJECTIVE
The aim of this study was to investigate the mandibular third molar pericoronitis flora by using real-time polymerase chain reaction (PCR).


METHODS
The quantitative values of Aggregatibacter actinomycetemcomitans (Aa), Campylobacter rectus (Cr), Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi) and Tannerella forsythia (Tf) were evaluated in comparison with the healthy third molar flora by using real time PCR.


RESULTS
Aa, Cr, Pg, and Pi were not statistically significant but numerically higher than the pericoronitis group. In contrast to samples from control subjects, statistically significant higher numbers of Tf were detected in samples from pericoronitis patients. The study revealed the strong relation between risk of pericoronitis and the presence of Tf. Individuals who have Tf in their samples present with an almost eight times relative risk of pericoronitis as the individuals with an absence of Tf in their samples.


CONCLUSIONS
Tf plays an important role in the development of clinical symptoms related to pericoronitis.
Department of Oral and Maxillofacial Surgery, Gülhane Military Medical Academy, Etlik, Ankara, Turkey.
Metin
M
Sencimen
Isil
I
Saygun
Aydin
A
Gulses
Vehbi
V
Bal
Cengiz H
CH
Acikel
Ayhan
A
Kubar
D000293:Adolescent; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D058491:Bacterial Load; D001439:Bacteroides / Q000302:isolation & purification; D001442:Bacteroides Infections / Q000382:microbiology; D044883:Campylobacter rectus / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D003774:Dental Plaque Index; D005260:Female; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D005884:Gingival Hemorrhage / Q000145:classification; D006090:Gram-Negative Bacteria / Q000145:classification*; D006801:Humans; D008297:Male; D008334:Mandible; D008964:Molar, Third / Q000382:microbiology*; D010497:Pericoronitis / Q000382:microbiology*; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification; D010519:Periodontium / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D018713:Prevotella intermedia / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012307:Risk Factors; D055815:Young Adult
D004269:DNA, Bacterial
Pericoronitis; periodontal bacteria; real-time polymerase chain reaction