Effects of PPAR-γ agonist treatment on LPS-induced mastitis in rats.
PPAR-γ, a member of the nuclear receptor superfamily, plays an important role in lipid metabolism and inflammation. The aim of this study was to investigate the preventive effects of synthetic PPAR-γ agonist rosiglitazone on lipopolysaccharide (LPS)-induced mastitis in rats. The mouse model of mastitis was induced by the injection of LPS through the duct of the mammary gland. Rosiglitazone was injected 1 h before the induction of LPS intraperitoneally. The results showed that rosiglitazone attenuated the infiltration of inflammatory cells, the activity of myeloperoxidase (MPO), and the production of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in a dose-dependent manner. Additionally, Western blotting showed that rosiglitazone inhibited the phosphorylation of IκB-α and NF-κB p65. These results indicated that rosiglitazone has a protective effect on mastitis, and the anti-inflammatory mechanism of rosiglitazone on LPS-induced mastitis in rats may be due to its ability to inhibit NF-κB signaling pathways. PPAR-γ may be a potential therapeutic target against mastitis.
Department of General Surgery, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, People's Republic of China.
Ding
D
Mingfeng
Ming
M
Xiaodong
Liu
L
Yue
Piao
P
Taikui
Xiao
X
Lei
Liu
L
Ming
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D008070:Lipopolysaccharides / Q000633:toxicity*; D008413:Mastitis / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D047495:PPAR gamma / Q000819:agonists* / Q000378:metabolism; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology / Q000627:therapeutic use*
D008070:Lipopolysaccharides; D047495:PPAR gamma; D045162:Thiazolidinediones; D000077154:Rosiglitazone