[Platelet derived growth factor receptor β over-expression in endothelial progenitor cells promote reendothelialization after vascular injury].
OBJECTIVE
To observe the effect of platelet derived growth factor receptor β (PDGFR-β) transfected endothelial progenitor cells (EPCs) on vascular regeneration.


METHODS
Spleen-derived mononuclear cells (MNCs) were isolated using density gradient centrifugation and induced with special culture medium. EPCs transfection was performed with Lipofectamine(TM) 2000 reagent according to the instruction manual. Carotid artery injury was induced in splenectomized mice. EPCs were injected by tail vein immediately and at 24 h after endothelial injury of the carotid artery. Evans blue staining was performed to evaluate reendothelialization at 7 days after endothelial injury of the carotid artery.


RESULTS
Most adherent cells were LDL and UEA-I double positive. Laser scanning confocal microscopy showed that transfection efficiency was about 50%-60%. The reendothelialized area in the PDGFR-β-EPCs group was significantly larger than that in EGFP-EPCs group.


CONCLUSIONS
Transplantation of PDGFR-β over-expressed EPCs can promote reendothelialization in the early phase after carotid artery injury.
Second Department of Geratology, Wuhan General Hospital of Guangzhou Command, Wuhan 430070, China.
Hang
H
Wang
Hao
H
Huang
Yangguang
Y
Yin
Mengyang
M
Deng
Huali
H
Kang
Email: huanglan260@126.com.
Lan
L
Huang
D000818:Animals; D020212:Carotid Artery Injuries / Q000473:pathology / Q000601:surgery*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D004730:Endothelium, Vascular / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics* / Q000378:metabolism; D012038:Regeneration; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D017479:Receptors, Platelet-Derived Growth Factor