Detection of protein arginine methylation in Saccharomyces cerevisiae.
Protein arginine methylation has emerged to be an important regulator of cellular protein functions. Techniques that uncover the presence of methylarginines on a protein provide an important step towards understanding the functional role of arginine methylation. Here, we describe several common methods used to detect the presence of protein arginine methylation in Saccharomyces cerevisiae.
Department of Biological Sciences, The State University of New York at Buffalo, 109 Cooke Hall, Buffalo, NY, 14260, USA.
Christopher A
CA
Jackson
Michael C
MC
Yu
D000595:Amino Acid Sequence; D001120:Arginine / Q000378:metabolism*; D008745:Methylation; D008967:Molecular Biology / Q000379:methods*; D012097:Repressor Proteins / Q000235:genetics / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
D012097:Repressor Proteins; D001120:Arginine