Potential of biogenic hydrogen production for hydrogen driven remediation strategies in marine environments.
Fermentative production of bio-hydrogen (bio-H2) from organic residues has emerged as a promising alternative for providing the required electron source for hydrogen driven remediation strategies. Unlike the widely used production of H2 by bacteria in fresh water systems, few reports are available regarding the generation of biogenic H2 and optimisation processes in marine systems. The present research aims to optimise the capability of an indigenous marine bacterium for the production of bio-H2 in marine environments and subsequently develop this process for hydrogen driven remediation strategies. Fermentative conversion of organics in marine media to H2 using a marine isolate, Pseudoalteromonas sp. BH11, was determined. A Taguchi design of experimental methodology was employed to evaluate the optimal nutritional composition in batch tests to improve bio-H2 yields. Further optimisation experiments showed that alginate-immobilised bacterial cells were able to produce bio-H2 at the same rate as suspended cells over a period of several weeks. Finally, bio-H2 was used as electron donor to successfully dehalogenate trichloroethylene (TCE) using biogenic palladium nanoparticles as a catalyst. Fermentative production of bio-H2 can be a promising technique for concomitant generation of an electron source for hydrogen driven remediation strategies and treatment of organic residue in marine ecosystems.
Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, Gent B-9000, Belgium; BIOMED, University of Hasselt, Agoralaan Building C, Diepenbeek, Belgium.
Baharak
B
Hosseinkhani
Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, Gent B-9000, Belgium; Department of Civil and Environmental Engineering, University of California at Berkeley, Berkeley, CA 94720, USA.
Tom
T
Hennebel
Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, Gent B-9000, Belgium. Electronic address: Nico.Boon@UGent.be.
Nico
N
Boon
D000464:Alginates / Q000737:chemistry; D059001:Aquatic Organisms / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018914:Cells, Immobilized / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006859:Hydrogen / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*; D031161:Pseudoalteromonas / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology
D000464:Alginates; D006603:Hexuronic Acids; D010165:Palladium; D006859:Hydrogen; D020723:Glucuronic Acid