The effect of borneol on the concentration of meropenem in rat brain and blood.
Meropenem is a carbapenem antibiotic with a wide spectrum of activity against both Gram-positive and Gram-negative bacteria. Because of its clinical efficacy, meropenem is an excellent choice for the treatment of serious infections in both adults and children. The knowledge of tissue concentrations of antibiotic in an infection site is valuable for the prediction of treatment outcome. The aim of the present study is to investigate the effect of borneol on the concentration of meropenem in rat brain and blood and to find the potential relationships of the combined use of medicine and traditional Chinese medicine. Analysis of meropenem in the dialysates was achieved using the microdialysis technique and HPLC. At 40 min after the administration of an intraperitoneal injection of meropenem, the concentration of meropenem in brain in borneol+meropenem group was 2.25 (0.35) μg ml(-1), which was significantly higher than that in meropenem group [1.20 (0.12) μg ml(-1); P < 0.01]. Within 80 min of drug administration, the AUCbrain/AUCblood (area under the curve, AUC) in the borneol+meropenem group was 1.2 times that of the meropenem group. Borneol can increase the concentration of meropenem in the cerebrospinal fluid, but has no influence on its blood concentration. This study represents a successful application of the microdialysis technique, which is an effective method for the study of pharmacokinetics of meropenem.
a Department of Pharmaceutical Care , The General Hospital of the People's Liberation Army , Beijing 100853 , China.
Hai-Li
HL
Xin
Xin-Rong
XR
He
Wei
W
Li
Zhen-Dong
ZD
Zhou
Shuo
S
Zhang
Guan-Jun
GJ
Wang
D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D001812:Blood-Brain Barrier / Q000187:drug effects; D001921:Brain / Q000187:drug effects; D001892:Camphanes / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D002648:Child; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D000077731:Meropenem; D017551:Microdialysis; D015394:Molecular Structure; D051381:Rats; D017208:Rats, Wistar; D013845:Thienamycins / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*
D000900:Anti-Bacterial Agents; D001892:Camphanes; D013845:Thienamycins; D000077731:Meropenem; C022871:isoborneol
blood microdialysis; blood–brain barrier; borneol; brain microdialysis; meropenem