Comparison of the degree of autophagy in neonatal rat cardiomyocytes and H9c2 cells exposed to hypoxia/reoxygenation.
BACKGROUND
Hypoxia/reoxygenation (H/R) is an important in vitro model for exploring the molecular mechanisms and functions of autophagy during myocardial ischemia/reperfusion (I/R). Neonatal rat cardiomyocytes (NRCM) and H9c2 cells are widely used to study H/R.


METHODS
The degree of autophagy in NRCM and H9c2 cells exposed to H/R was assessed by detecting the markers of autophagy, Beclin-1 and LC3II. Autophagosomes were confirmed in both NRCM and H9c2 cells exposed to H/R using MDC staining and TEM.


RESULTS
The expression levels of Beclin-1 and LC3II were significantly increased in NRCM under H/R conditions (p < 0.05 vs. control). In contrast, the expression levels of Beclin-1 and LC3II were significantly reduced in H9c2 cells exposed to H/R (p < 0.05 vs. control). The fluorescence intensity and the number of MDC-labeled particles were greater in NRCM exposed to H/R, compared to H9c2 cells (p < 0.05 vs. control). The number of autophagosomes exposed to H/R by TEM was greater in NRCM, compared to H9c2 cells, which was similar to the levels of autophagy markers observed in NRCM and H9c2 cells (p < 0.05 vs. control).


CONCLUSIONS
NRCM may be more suitable to study autophagy during H/R than H9c2 cells.
Xueming
X
Cao
Xianbao
X
Wang
Yuanna
Y
Ling
Xudong
X
Song
Pingzhen
P
Yang
Yingfeng
Y
Liu
Lei
L
Liu
Lizi
L
Wang
Jingbin
J
Guo
Aihua
A
Chen
D000818:Animals; D000831:Animals, Newborn; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism / Q000648:ultrastructure; D001343:Autophagy; D000071186:Beclin-1; D002460:Cell Line; D004195:Disease Models, Animal; D000860:Hypoxia / Q000473:pathology*; D046529:Microscopy, Electron, Transmission; D008869:Microtubule-Associated Proteins / Q000378:metabolism / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000473:pathology* / Q000648:ultrastructure; D010100:Oxygen / Q000502:physiology*; D051381:Rats
D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; D008869:Microtubule-Associated Proteins; D010100:Oxygen; C512829:Becn1 protein, rat; C526109:Anxa3 protein, rat