title: [The effect of 7-ketocholesterol on surface ICAM-1 and PECAM-1 expression in human aortic endothelial cells].
abstract: Proatherogenic factors lead to activation of endothelial cells, which symptom is an increased expression of surface adhesion molecules enabling the initiation of a local inflammatory response. 7-ketocholesterol (7-KCH) is a product of oxidation of cholesterol with proven pro-apoptotic effect on the cells of the vessel wall. So far, however, the impact of 7-KCH on surface expression of adhesion molecules has not been assessed. The aim of this study was to evaluate the influence of 7-KCH on the surface expression of adhesion molecules--intracellular adhesion molecule 1 (ICAM-1, CD 54) and .platelet endothelial cell adhesion molecule 1 (PECAM-1, CD31) on human aortic endothelial cells (HAECs).
  
  
  METHODS
  After treatment with 7-KCH surface expression of adhesion molecules on HAEC was measured with antihuman CD31 and anti-human CD54 antibodies using flow cytometer.
  
  
  RESULTS
  7-KCH significantly increases percentages of CD 54 on viable HEAC, but does not affect expression of CD 31.
  
  
  CONCLUSIONS
  7-KCH may enhance the initiation of a local inflammatory response in atherosclerosis by increasing the expression of ICAM-1.
authors.forename: Roksana
authors.initials: R
authors.lastname: Dorantowicz
authors.forename: Emilia
authors.initials: E
authors.lastname: Łuczak
authors.forename: Marlena
authors.initials: M
authors.lastname: Broncel
mesh_terms: D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007653:Ketocholesterols / Q000378:metabolism* / Q000494:pharmacology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000187:drug effects / Q000378:metabolism*
chemical_list: D007653:Ketocholesterols; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; C003001:7-ketocholesterol