title: A case-control study of the role of human papillomavirus in oesophageal squamous cell carcinoma in australia.
abstract: Objective. We investigate the prevalence of human papillomavirus (HPV) in oesophageal squamous cell carcinoma (OSCC) tissues compared to oesophageal tissue from healthy controls, in an Australian cohort. Methods. We conducted a hospital-based case-control study of 99 patients with OSCC and 100 healthy controls to examine the presence of HPV DNA. Paraffin tissues were tested using the PapType high-risk HPV detection and genotyping kit and with INNO-LiPA HPV Genotyping Extra. The biopsy samples were tested for HPV using a PCR-ELISA method based on the L1 consensus primer set PGMY09-PGMY11. Results. HPV DNA of the oncogenic genotype 16 was detected in 1/99 case specimens, a rate of 1010 per 100,000 (95% CI: 30-5500). All control specimens were negative for HPV. Significantly higher rates of smoking, other aerodigestive cancers, and mortality were seen among cases than controls. A pooled analysis of this study and the only other Australian case-control study found that 9/321 cases and 0/155 controls were positive for HPV. The pooled odds ratio for HPV being a risk factor for OSCC was 9.35 (95% CI: 0.47-190.33). Conclusion. Our results suggest that in this multifactorial cancer HPV may be an additional risk factor; although a larger, better powered study is needed.
authors.affiliation: School of Public Health and Community Medicine, The University of New South Wales, Kensington, NSW 2052, Australia.
authors.forename: Surabhi S
authors.initials: SS
authors.lastname: Liyanage
authors.affiliation: Department of Medicine, St. Vincent's Hospital Clinical School, The University of New South Wales, Kensington, NSW 2052, Australia.
authors.forename: Eva
authors.identifier: 0000-0002-4410-6144
authors.initials: E
authors.lastname: Segelov
authors.affiliation: UNSW Medicine, The University of New South Wales, Kensington, NSW 2052, Australia.
authors.forename: Aisha
authors.initials: A
authors.lastname: Malik
authors.affiliation: Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, VIC 3052, Australia ; Department of Obstetrics and Gynecology, Murdoch Children's Research Institute, University of Melbourne, Parkville, VIC 3010, Australia.
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Garland
authors.affiliation: Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, VIC 3052, Australia ; Department of Obstetrics and Gynecology, Murdoch Children's Research Institute, University of Melbourne, Parkville, VIC 3010, Australia.
authors.forename: Sepehr N
authors.initials: SN
authors.lastname: Tabrizi
authors.affiliation: Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, VIC 3052, Australia ; Department of Obstetrics and Gynecology, Murdoch Children's Research Institute, University of Melbourne, Parkville, VIC 3010, Australia.
authors.forename: Eleanor
authors.identifier: 0000-0002-6293-9043
authors.initials: E
authors.lastname: Cummins
authors.affiliation: School of Public Health and Community Medicine, The University of New South Wales, Kensington, NSW 2052, Australia.
authors.forename: Holly
authors.identifier: 0000-0002-1877-5395
authors.initials: H
authors.lastname: Seale
authors.affiliation: School of Public Health and Community Medicine, The University of New South Wales, Kensington, NSW 2052, Australia.
authors.forename: Bayzidur
authors.initials: B
authors.lastname: Rahman
authors.affiliation: School of Public Health and Community Medicine, The University of New South Wales, Kensington, NSW 2052, Australia.
authors.forename: Aye
authors.identifier: 0000-0003-4274-6241
authors.initials: A
authors.lastname: Moa
authors.affiliation: Department of Surgery, Princess Alexandra Hospital, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Barbour
authors.affiliation: Department of Surgery, Prince of Wales Hospital Clinical School, The University of New South Wales, Kensington, NSW 2052, Australia.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Crowe
authors.affiliation: School of Public Health and Community Medicine, The University of New South Wales, Kensington, NSW 2052, Australia.
authors.forename: C Raina
authors.initials: CR
authors.lastname: MacIntyre