title: Dietary supplementation with resveratrol protects against striatal dopaminergic deficits produced by in utero LPS exposure.
abstract: The purpose of this study was to determine the effect of dietary supplementation with the anti-inflammatory compound resveratrol in pregnant dams on lipopolysaccharide (LPS)-induced dopaminergic deficits in pups exposed to LPS in utero. Gravid female rats were fed with a resveratrol-enriched diet during gestational days 3-22.5 (E3-E22.5) and received an intraperitoneal (i.p.) injection of 1mg/kg LPS at E10.5. The striata were isolated from the pups at postnatal days 10 (P10) and P21. LPS-induced dopaminergic deficits were noted at P21, but not P10. These DA deficits at P21 were exhibited by a loss of DA and DA metabolite [3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA)] levels and tyrosine hydroxylase (TH) expression in the striatum. The LPS-induced loss of DA, DA metabolites, and TH expression were attenuated in the striata of pups from the dams fed with the resveratrol-supplemented diet. These data suggest that a resveratrol-supplemented diet may restore homeostasis of the striatal DA neuronal system following disruption by LPS.
authors.affiliation: Division of Pharmacology, Graduate School of Pharmaceutical Sciences, Duquesne University, 600 Forbes Avenue, Pittsburgh, PA 15282, USA.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Rose
authors.affiliation: Division of Pharmacology, Graduate School of Pharmaceutical Sciences, Duquesne University, 600 Forbes Avenue, Pittsburgh, PA 15282, USA.
authors.forename: Mayur S
authors.initials: MS
authors.lastname: Parmar
authors.affiliation: Division of Pharmacology, Graduate School of Pharmaceutical Sciences, Duquesne University, 600 Forbes Avenue, Pittsburgh, PA 15282, USA. Electronic address: cavanaughj@duq.edu.
authors.forename: Jane E
authors.initials: JE
authors.lastname: Cavanaugh
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D003342:Corpus Striatum / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism*; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism; D005260:Female; D006719:Homovanillic Acid / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology*; D039382:Maternal Nutritional Physiological Phenomena; D009474:Neurons / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000178:diet therapy*; D011297:Prenatal Exposure Delayed Effects / Q000517:prevention & control*; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D013267:Stilbenes; D015102:3,4-Dihydroxyphenylacetic Acid; D014446:Tyrosine 3-Monooxygenase; D000077185:Resveratrol; D004298:Dopamine; D006719:Homovanillic Acid
keywords: Dopamine; Lipopolysaccharide; Postnatal development; Resveratrol; Striatum; Tyrosine hydroxylase