title: Inhibitory effects of Baccharis dracunculifolia on 1,2-dimethylhidrazine-induced genotoxicity and preneoplastic lesions in rat colon.
abstract: Baccharis dracunculifolia (Asteraceae), the main botanical source of green propolis, also known as 'alecrim-do-campo' and 'vassourinha', is a shrub of the Brazilian 'cerrado' and is native to the South and Southeast of Brazil. The effects of B. dracunculifolia ethyl acetate extract (Bd-EAE) were evaluated on the 1,2-dimethylhydrazine (DMH)-induced DNA damage and aberrant crypt foci (ACF) in the colon of male Wistar rats by the comet and ACF assays, respectively. The animals were treated by gavage with doses of 6, 12, and 24 mg/kg body weight/day. Animals were also administered a single subcutaneous injection of 40 mg/kg DMH and were killed after 4 h for evaluation of DNA damage. Also, two doses of 40 mg/kg of DMH were administered weekly for 2 weeks, and animals were killed 2 weeks after the last injection for evaluation of ACF development in the colon. The results showed a significant reduction in the frequency of DNA damage and ACF in the group treated with the Bd-EAE plus DMH in comparison with those treated with DMH alone, suggesting that Bd-EAE reduced DNA damage and suppressed the formation of ACF and also exerted a protective affect against colon carcinogenesis.
authors.affiliation: aUniversidade de Franca, Franca bFaculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
authors.forename: Carla C
authors.initials: CC
authors.lastname: Munari
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Furtado
authors.forename: Mirian L
authors.initials: ML
authors.lastname: Santiago
authors.forename: Simony S
authors.initials: SS
authors.lastname: Manhas
authors.forename: Jairo K
authors.initials: JK
authors.lastname: Bastos
authors.forename: Denise C
authors.initials: DC
authors.lastname: Tavares
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000633:toxicity*; D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D031175:Baccharis; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000473:pathology; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D008297:Male; D009374:Neoplasms, Experimental; D010936:Plant Extracts / Q000494:pharmacology*; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000477:Alkylating Agents; D010936:Plant Extracts; D019813:1,2-Dimethylhydrazine