title: Visualizing the endocytosis of phenylephrine in living cells by quantum dot-based tracking.
abstract: To study the intracellular receptor-drug transportation, a fluorescent probe consisting of phenylephrine-polyethylene glycol-quantum dots conjugate was employed to track endocytosis process of phenylephrine in living cells. This type of movement was studied by continuously filming fluorescent images in the same cell. We also calculated the movement parameters, and divided the endocytosis process into 6 stages. Furthermore, the movement parameters of this probe in different organelles were determined by co-localization of the probe fluorescent images and different cellular organelles. After comparing the parameters in cellular organelles with these in 6 stages, the whole endocytosis pathway was demonstrated. These results verified that this probe successfully tracked the whole intracellular dynamic endocytosis process of phenylephrine. Our method realized the visual tracking the whole receptor-mediated endocytosis, which is a new approach on investigating the molecular mechanisms and kinetic properties of intracellular receptor-drug transportation.
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmacy, Shandong University, Wenhua Xilu 44, 250012 Jinan, Shandong Province, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmacy, Shandong University, Wenhua Xilu 44, 250012 Jinan, Shandong Province, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmacy, Shandong University, Wenhua Xilu 44, 250012 Jinan, Shandong Province, PR China.
authors.forename: Zhun
authors.initials: Z
authors.lastname: Hou
authors.affiliation: Institute of Vascular Medicine, Peking University Third Hospital, 49 North Garden Rd., 100191 Haidian District, Beijing, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Song
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmacy, Shandong University, Wenhua Xilu 44, 250012 Jinan, Shandong Province, PR China. Electronic address: wangl-sdu@sdu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Shanda Nanlu 27, 250100 Jinan, Shandong Province, PR China. Electronic address: wjiang@sdu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
mesh_terms: D004705:Endocytosis; D011992:Endosomes / Q000737:chemistry; D057809:HEK293 Cells; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D008247:Lysosomes / Q000737:chemistry; D015388:Organelles / Q000378:metabolism; D010656:Phenylephrine / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014404:Tubulin / Q000737:chemistry
chemical_list: D014404:Tubulin; D010656:Phenylephrine; D011092:Polyethylene Glycols
keywords: Endocytosis; Living cells; Phenylephrine; Quantum dots; Tracking