title: Relative mRNA expression and immunolocalization for transforming growth factor-beta (TGF-β) and their effect on in vitro development of caprine preantral follicles.
abstract: This study aimed to evaluate the immunolocalization and messenger RNA (mRNA) expression for transforming growth factor-beta (TGF-β) and its receptors (TGF-βRI and RII), as well as mRNA expression for P450 aromatase and FSH receptor in caprine preantral follicles. The effects of TGF-β, FSH alone, or in association on the in vitro follicular development were also assessed. Immunohistochemical analyses showed the expression of TGF-β and its receptors in oocytes of all follicle stages and granulosa cells of primary and secondary follicles. mRNA for TGF-β receptors and for FSH receptor (FSHR) was present in preantral follicles as well as in oocytes and granulosa cells of antral follicles. Isolated secondary follicles were cultured in α-minimum essential medium (MEM) alone or supplemented with either FSH (100 ng/ml), TGF-β (10 ng/ml), or TGF-β + FSH for 18 d. TGF-β increased significantly oocyte diameter when compared to FSH alone and control. After 18 d of culture, all groups showed a significant reduction in P450 aromatase and FSHR mRNA levels in comparison to fresh control. In contrast, treatment with FSH significantly increased the mRNA expression for TGF-β in comparison to fresh control and other treatments. In conclusion, the findings showed that TGF-β and its receptors are present in caprine ovarian follicles. Furthermore, they showed a positive effect on oocyte growth in vitro.
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil, gqrvet@hotmail.com.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Rodrigues
authors.forename: M J
authors.initials: MJ
authors.lastname: Bertoldo
authors.forename: I R
authors.initials: IR
authors.lastname: Brito
authors.forename: C M G
authors.initials: CM
authors.lastname: Silva
authors.forename: A D
authors.initials: AD
authors.lastname: Sales
authors.forename: S V
authors.initials: SV
authors.lastname: Castro
authors.forename: N
authors.initials: N
authors.lastname: Duffard
authors.forename: Y
authors.initials: Y
authors.lastname: Locatelli
authors.forename: P
authors.initials: P
authors.lastname: Mermillod
authors.forename: C H
authors.initials: CH
authors.lastname: Lobo
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.forename: A P R
authors.initials: AP
authors.lastname: Rodrigues
authors.forename: V J F
authors.initials: VJ
authors.lastname: Freitas
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D003470:Culture Media; D005260:Female; D006041:Goats; D066298:In Vitro Techniques; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000502:physiology; D011509:Proteoglycans / Q000096:biosynthesis / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II; D011962:Receptors, FSH / Q000096:biosynthesis / Q000502:physiology; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000502:physiology
chemical_list: D003470:Culture Media; D011509:Proteoglycans; D012333:RNA, Messenger; D011962:Receptors, FSH; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; C071736:betaglycan; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II