title: Exploring genome wide bisulfite sequencing for DNA methylation analysis in livestock: a technical assessment.
abstract: Recent advances made in "omics" technologies are contributing to a revolution in livestock selection and breeding practices. Epigenetic mechanisms, including DNA methylation are important determinants for the control of gene expression in mammals. DNA methylation research will help our understanding of how environmental factors contribute to phenotypic variation of complex production and health traits. High-throughput sequencing is a vital tool for the comprehensive analysis of DNA methylation, and bisulfite-based strategies coupled with DNA sequencing allows for quantitative, site-specific methylation analysis at the genome level or genome wide. Reduced representation bisulfite sequencing (RRBS) and more recently whole genome bisulfite sequencing (WGBS) have proven to be effective techniques for studying DNA methylation in both humans and mice. Here we report the development of RRBS and WGBS for use in sheep, the first application of this technology in livestock species. Important technical issues associated with these methodologies including fragment size selection and sequence depth are examined and discussed.
authors.affiliation: Animal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre Teagasc, Grange, County Meath, Ireland.
authors.forename: Rachael
authors.initials: R
authors.lastname: Doherty
authors.affiliation: Reproductive Technologies, AgResearch Ruakura Research Centre Hamilton, New Zealand.
authors.forename: Christine
authors.initials: C
authors.lastname: Couldrey
keywords: DNA methylation; RRBS; WGBS; epigenetics; fragment size; quantification; sheep