title: Extracts from Curcuma zedoaria Inhibit Proliferation of Human Breast Cancer Cell MDA-MB-231 In Vitro.
abstract: Objective. To evaluate the effect of petroleum ether extracts of Curcuma zedoaria on the proliferation of human triple negative breast cancer cell line MDA-MB-231. Methods. The reagents were isolated from Curcuma zedoaria by petroleum ether fraction. It was assayed by CCK8 for MDA-MB-231 cellular viability with various concentrations and days, cell cycle analyses, Western Blot analysis, and Realtime Reverse Transcriptase PCR analyses for chemokines molecules including E-cadherin, and E-selectin, and adhesion molecules including CCR7, SLC, SDF-1, and CXCR4. Epirubicin was used as control in the study. Results. MDA-MB-231 cells were inhibited by petroleum ether extracts of Curcuma zedoaria (P < 0.05), and the inhibition rate was dependent on concentrations and time. Petroleum ether extracts of Curcuma zedoaria as well as Epirubicin produce a significant G0/G1 cell cycle arrest. The level of expression of proteins E-cadherin and E-cadherin mRNA was significantly increased, while proteins SDF-1, CCR7, and CXCR4 mRNA were decreased after being incubated with petroleum ether extracts of Curcuma zedoaria at the concentrations of 300  μ g/mL than control (P < 0.05). The differences were that the protein CXCR4 mRNA expression level was higher than vehicle. Conclusions. MDA-MB-231 cells were inhibited by petroleum ether extracts of Curcuma zedoaria.
authors.affiliation: The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.
authors.forename: Xiu-Fei
authors.initials: XF
authors.lastname: Gao
authors.affiliation: Zhejiang Cancer Hospital, Zhejiang 310022, China.
authors.forename: Qing-Lin
authors.initials: QL
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Li
authors.affiliation: Zhejiang Cancer Hospital, Zhejiang 310022, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Su
authors.affiliation: The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.affiliation: Zhejiang Cancer Hospital, Zhejiang 310022, China.
authors.forename: Ai-Qin
authors.initials: AQ
authors.lastname: Zhang