title: PLCE1 mRNA and protein expression and survival of patients with esophageal squamous cell carcinoma and gastric adenocarcinoma.
abstract: BACKGROUND
  Germline genetic variants in PLCE1 (10q23) have demonstrated consistent associations with risk of esophageal squamous cell carcinoma (ESCC) and gastric cancer among Chinese. We evaluated PLCE1 mRNA and protein expression in paired tumor-normal tissues, and their relationship with survival.
  
  
  METHODS
  PLCE1 mRNA was profiled using three probes in the Affymetrix GeneChip U133 for paired tumor-normal tissues of ESCC (n = 132), gastric cardia adenocarcinoma (GCA, n = 62), and gastric noncardia adenocarcinoma (GNCA, n = 72). We used immunohistochemistry to detect PLCE1 protein on slides from tissue microarrays in paired tumor-normal tissues of ESCC (n = 303), and tumors of GCA (n = 298) and GNCA (n = 124).
  
  
  RESULTS
  Compared with normal tissues, PLCE1 mRNA expression was significantly reduced in ESCC tumors (P = 0.03, probe_205112_at), as well as in GCA and GNCA tumors (P < 0.0001, each probe). Protein expression was nonsignificantly reduced in ESCC tumors (P = 0.51). Increased tumor-normal mRNA fold change (probe_205112_at) was associated with longer survival in ESCC (9.6 months for highest vs. lowest quartile; Ptrend = 0.02). Increased mRNA tumor-normal fold change (probe_205111_at) was associated with longer survival for GCA (10.7 months for highest quartile; Ptrend = 0.04), but not for GNCA cases (P = 0.72). Similar to mRNA, elevated tumor-normal fold change for protein in ESCC was also associated with improved survival (8.1 months for highest quartile; Ptrend = 0.04).
  
  
  CONCLUSIONS
  Dysregulated PLCE1 mRNA expression was observed for both ESCC (one probe only) and GCA tumors, and the altered PLCE1 expression seems to be associated with cancer prognosis.
  
  
  CONCLUSIONS
  A potential role for PLCE1 in the early detection and/or therapy of ESCC and GCA warrants further investigation.
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institute of Health, Bethesda, MD 20852.
authors.forename: Wen-Qing
authors.initials: WQ
authors.lastname: Li
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institute of Health, Bethesda, MD 20852.
authors.forename: Nan
authors.initials: N
authors.lastname: Hu
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institute of Health, Bethesda, MD 20852.
authors.forename: Victoria H
authors.initials: VH
authors.lastname: Burton
authors.affiliation: Office of the Director, Center for Cancer Research, NCI, Bethesda, MD.
authors.forename: Howard H
authors.initials: HH
authors.lastname: Yang
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institute of Health, Bethesda, MD 20852.
authors.forename: Hua
authors.initials: H
authors.lastname: Su
authors.affiliation: Tissue Array Research Program, Laboratory of Pathology, National Cancer Institute, Bethesda, MD.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Conway
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institute of Health, Bethesda, MD 20852.
authors.forename: Lemin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institute of Health, Bethesda, MD 20852.
authors.forename: Chaoyu
authors.initials: C
authors.lastname: Wang
authors.affiliation: Shanxi Cancer Hospital, Taiyuan, Shanxi, PR China.
authors.forename: Ti
authors.initials: T
authors.lastname: Ding
authors.affiliation: Shanxi Cancer Hospital, Taiyuan, Shanxi, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Information Management Services, Inc., Silver Spring, MD.
authors.forename: Carol
authors.initials: C
authors.lastname: Giffen
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institute of Health, Bethesda, MD 20852.
authors.forename: Christian C
authors.initials: CC
authors.lastname: Abnet
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institute of Health, Bethesda, MD 20852.
authors.forename: Alisa M
authors.initials: AM
authors.lastname: Goldstein
authors.affiliation: Tissue Array Research Program, Laboratory of Pathology, National Cancer Institute, Bethesda, MD.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Hewitt
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institute of Health, Bethesda, MD 20852.
authors.forename: Philip R
authors.initials: PR
authors.lastname: Taylor
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000401:mortality*; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000401:mortality*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000401:mortality*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D020411:Oligonucleotide Array Sequence Analysis; D054801:Phosphoinositide Phospholipase C / Q000032:analysis / Q000096:biosynthesis*; D020641:Polymorphism, Single Nucleotide; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000401:mortality*; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D054801:Phosphoinositide Phospholipase C; C423320:phospholipase C epsilon