title: Measurement of fatty acid oxidation rates in animal tissues and cell lines.
abstract: While much oncological research has focused on metabolic shifts in glucose and amino acid oxidation, recent evidence suggests that fatty acid oxidation (FAO) may also play an important role in the metabolic reprogramming of cancer cells. Here, we present a simple method for measuring FAO rates using radiolabeled palmitate, common laboratory reagents, and standard supplies. This protocol is broadly applicable for measuring FAO rates in cultured cancer cells as well as in both malignant and nontransformed animal tissues.
authors.affiliation: Duke Molecular Physiology Institute, Duke University Medical Center, Durham, North Carolina, USA.
authors.forename: Frank K
authors.initials: FK
authors.lastname: Huynh
authors.affiliation: Duke Molecular Physiology Institute, Duke University Medical Center, Durham, North Carolina, USA.
authors.forename: Michelle F
authors.initials: MF
authors.lastname: Green
authors.affiliation: Duke Molecular Physiology Institute, Duke University Medical Center, Durham, North Carolina, USA; Sarah W. Stedman Nutrition and Metabolism Center, Duke University Medical Center, Durham, North Carolina, USA; Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA.
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Koves
authors.affiliation: Duke Molecular Physiology Institute, Duke University Medical Center, Durham, North Carolina, USA; Sarah W. Stedman Nutrition and Metabolism Center, Duke University Medical Center, Durham, North Carolina, USA; Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA; Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, USA. Electronic address: matthew.hirschey@duke.edu.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Hirschey
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D002250:Carbon Radioisotopes; D002460:Cell Line; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism*; D010084:Oxidation-Reduction; D010168:Palmitates; D014407:Tumor Cells, Cultured
chemical_list: D002250:Carbon Radioisotopes; D005227:Fatty Acids; D010168:Palmitates
keywords: Acid-soluble metabolite; Cell lysate; Radiolabeling; Xenografts; β-Oxidation CO(2)