title: Distribution of glutathione peroxidase 1 in liver tissues of healthy and diabetic rats treated with capsaisin.
abstract: We investigated the immunohistochemical localization of glutathione peroxidase 1 (GPx 1) and the structural changes that occur in the livers of healthy and diabetic rats that were treated with capsaisin (CAP). Fifty female rats were divided into five groups: group 1, sham; group 2, untreated control; group 3, CAP-treated; group 4, streptozotocin (STZ) diabetic; group 5, STZ diabetic + CAP-treated. STZ was administered to groups 4 and 5; after verifying diabetes, CAP was administered daily for 2 weeks to groups 3 and 5. Diffuse, microvesicular and some macrovesicular fatty degeneration were observed in the cytoplasms of hepatocytes in the livers of the diabetic group. In the CAP-treated diabetic group, fat degeneration in the livers decreased slightly by day 7. Irregularity of the external contours of nuclei of the hepatocytes, swelling of the nuclei, and slight anisocytosis and anisokaryosis were observed in the hepatocytes of the diabetic group. In the CAP-treated diabetic groups, the severity of anisocytosis and anisokaryosis decreased slightly by day 7. In all groups, GPx 1 showed similar immunolocalization, but in the diabetic and diabetic + CAP groups, GPx 1 immunoreactivity was less than in the other groups. GPx 1 immunoreactivity in the CAP-treated diabetic group was weaker than in the diabetic group. In all groups, GPx 1 immunoreactivity was diffusely cytoplasmic in some of the hepatocytes, and diffusely cytoplasmic and diffusely nuclear in other hepatocytes. Also, GPx 1 immunoreactivity in the liver was more intense in the hepatocytes around Kiernan's space. We found that CAP caused a decrease in GPx 1.
authors.affiliation: Department of Histology-Embryology, Faculty of Veterinary Medicine, University of Kafkas.
authors.forename: T
authors.initials: T
authors.lastname: Deprem
authors.forename: S E
authors.initials: SE
authors.lastname: Yıldız
authors.forename: E K
authors.initials: EK
authors.lastname: Sari
authors.forename: S A
authors.initials: SA
authors.lastname: Bingol
authors.forename: S K
authors.initials: SK
authors.lastname: Tasci
authors.forename: S
authors.initials: S
authors.lastname: Aslan
authors.forename: M
authors.initials: M
authors.lastname: Sozmen
authors.forename: G
authors.initials: G
authors.lastname: Nur
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000201:enzymology*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D022781:Hepatocytes / Q000201:enzymology*; D008099:Liver / Q000201:enzymology* / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000494:pharmacology; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D013311:Streptozocin; D005979:Glutathione Peroxidase; D002211:Capsaicin; D000094765:Glutathione Peroxidase GPX1; C000722069:Gpx1 protein, rat
keywords: GPx 1; capsaisin; diabetes mellitus; glutathione peroxidase 1; immunohistochemistry; liver