title: CO2 biofixation and carbonic anhydrase activity in Scenedesmus obliquus SA1 cultivated in large scale open system.
abstract: The present study deals with the large scale open system cultivation of the novel microalga: Scenedesmus obliquus SA1 (KC733762) previously isolated in our laboratory. SA1 strain was cultivated in open system at varying CO2 levels ranging from 0.03% to 35% (v/v) and subsequently the carbonic anhydrase activity (CA) and the biochemical properties were monitored. Maximum biomass concentration (1.39 ± 0.023 g L(-1)), CO2 fixation rate (97.65 ± 1.03 mg L(-1)d(-1)) and total CA activity (166.86 ± 3.30 E.U./mg chla) were obtained at 35% CO2. CA inhibitors: acetazolamide and ethoxyzolamide inhibited the external and internal enzyme activity in SA1. High CO2 levels were favorable for the accumulation of lipids and chlorophyll. The present results suggested that SA1 possessed high CO2 tolerance and high carbohydrate, lipid and chlorophyll content when cultivated in open system thus being suitable for CO2 mitigation in outdoor ponds and subsequent generation of value added products.
authors.affiliation: Centre for Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
authors.forename: Samarpita
authors.initials: S
authors.lastname: Basu
authors.affiliation: Centre for Energy, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
authors.forename: Abhijit Sarma
authors.initials: AS
authors.lastname: Roy
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
authors.forename: Kaustubha
authors.initials: K
authors.lastname: Mohanty
authors.affiliation: Centre for Energy, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India; Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address: aloke@iitg.ernet.in.
authors.forename: Aloke K
authors.initials: AK
authors.lastname: Ghoshal
mesh_terms: D018533:Biomass; D057486:Carbon Cycle / Q000187:drug effects; D002245:Carbon Dioxide / Q000378:metabolism* / Q000494:pharmacology; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D007700:Kinetics; D044444:Scenedesmus / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development*; D013997:Time Factors
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases
keywords: CO(2) biofixation; Carbonic anhydrase; Microalgae; Open system