title: Carbon sink removal: Increased photosynthetic production of lactic acid by Synechocystis sp. PCC6803 in a glycogen storage mutant.
abstract: Deletion of pathways for carbon-storage in the cyanobacterium Synechocystis sp. PCC6803 has been suggested as a strategy to increase the size of the available pyruvate pool for the production of (heterologous) chemical commodities. Here we show that deletion of the pathway for glycogen synthesis leads to a twofold increased lactate production rate, under nitrogen-limited conditions, whereas impairment of polyhydroxybutyrate synthesis does not.
authors.affiliation: Photanol BV, Science Park 408, 1098 XH Amsterdam, the Netherlands.
authors.forename: Aniek D
authors.initials: AD
authors.lastname: van der Woude
authors.affiliation: Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands.
authors.forename: S Andreas
authors.initials: SA
authors.lastname: Angermayr
authors.affiliation: Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands.
authors.forename: Vinod
authors.initials: V
authors.lastname: Puthan Veetil
authors.affiliation: Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands.
authors.forename: Anna
authors.initials: A
authors.lastname: Osnato
authors.affiliation: Photanol BV, Science Park 408, 1098 XH Amsterdam, the Netherlands; Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands. Electronic address: K.J.Hellingwerf@uva.nl.
authors.forename: Klaas J
authors.initials: KJ
authors.lastname: Hellingwerf
mesh_terms: D057965:Carbon Sequestration / Q000235:genetics; D006003:Glycogen / Q000096:biosynthesis* / Q000235:genetics; D006885:Hydroxybutyrates / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D060847:Metabolic Engineering; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D010788:Photosynthesis / Q000235:genetics*; D046939:Synechocystis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D006885:Hydroxybutyrates; D019344:Lactic Acid; D006003:Glycogen; D009584:Nitrogen
keywords: Cyanobacteria; Glycogen; Lactate; Metabolic engineering; Polyhydroxybutyrate