title: A draft map of the human proteome.
abstract: The availability of human genome sequence has transformed biomedical research over the past decade. However, an equivalent map for the human proteome with direct measurements of proteins and peptides does not exist yet. Here we present a draft map of the human proteome using high-resolution Fourier-transform mass spectrometry. In-depth proteomic profiling of 30 histologically normal human samples, including 17 adult tissues, 7 fetal tissues and 6 purified primary haematopoietic cells, resulted in identification of proteins encoded by 17,294 genes accounting for approximately 84% of the total annotated protein-coding genes in humans. A unique and comprehensive strategy for proteogenomic analysis enabled us to discover a number of novel protein-coding regions, which includes translated pseudogenes, non-coding RNAs and upstream open reading frames. This large human proteome catalogue (available as an interactive web-based resource at http://www.humanproteomemap.org) will complement available human genome and transcriptome data to accelerate biomedical research in health and disease.
authors.affiliation: 1] McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [2] Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: 1] McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [2] Adrienne Helis Malvin Medical Research Foundation, New Orleans, Louisiana 70130, USA.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: 1] McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [2] Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: The Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada.
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authors.affiliation: The Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
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authors.affiliation: McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
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authors.affiliation: McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
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authors.affiliation: Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
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authors.affiliation: 1] McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [2] Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [3] Institute of Bioinformatics, International Tech Park, Bangalore 560066, India [4] Adrienne Helis Malvin Medical Research Foundation, New Orleans, Louisiana 70130, USA [5] The Sol Goldman Pancreatic Cancer Research Center, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA [6] Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA [7] Diana Helis Henry Medical Research Foundation, New Orleans, Louisiana 70130, USA.
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mesh_terms: D000328:Adult; D002478:Cells, Cultured; D030562:Databases, Protein; D005333:Fetus / Q000378:metabolism; D005583:Fourier Analysis; D020869:Gene Expression Profiling; D015894:Genome, Human / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D020407:Internet; D013058:Mass Spectrometry; D058977:Molecular Sequence Annotation; D016366:Open Reading Frames / Q000235:genetics; D009928:Organ Specificity; D014176:Protein Biosynthesis; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D021382:Protein Sorting Signals; D021381:Protein Transport; D020543:Proteome / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D011544:Pseudogenes / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics; D015203:Reproducibility of Results; D020506:Untranslated Regions / Q000235:genetics
chemical_list: D020033:Protein Isoforms; D021382:Protein Sorting Signals; D020543:Proteome; D022661:RNA, Untranslated; D020506:Untranslated Regions