title: The smart 2-(2-fluorobenzoyl)-N-(2-methoxyphenyl)hydrazinecarbothioamide functionalized as Ni(II) sensor in micromolar concentration level and its application in live cell imaging.
abstract: In recent years, fluorescent probes for the detection of environmentally and biologically important metal cations have received extensive attention for designing and development of fluorescent chemosensors. Herein, we report the photophysical results of 2-(2-fluorobenzoyl)-N-(2-methoxyphenyl) hydrazinecarbothioamide (4) functionalized as Ni (II) sensor in micromolar concentration level. Through fluorescence titration at 488 nm, we were confirmed that ligand 4 showed the remarkable emission by complexation between 4 and Ni (II) while it appeared no emission in case of the competitive ions (Cr(3+), Fe(2+), Co(2+), Ba(2+), Cu(2+), Ca(2+), Na(+), K(+), Cu(+), Cs(+)). Furthermore, ligand 4 exhibited no toxicity with precise cell permeability toward normal living cells using L929 cell lines in bio imaging experiment investigated through confocal fluorescence microscope. The non-toxic behavior of ligand 4 (assessed by MTT assay) and its ability to track the Ni(2+) in living cells suggest its possibility to use in biological system as nickel sensor.
authors.affiliation: Department of Chemistry, Kongju National University, Gongju, Chungnam, 314-701, Republic of Korea.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Saleem
authors.forename: Anser
authors.initials: A
authors.lastname: Ali
authors.forename: Chang-Shik
authors.initials: CS
authors.lastname: Choi
authors.forename: Bong Joo
authors.initials: BJ
authors.lastname: Park
authors.forename: Eun Ha
authors.initials: EH
authors.lastname: Choi
authors.forename: Ki Hwan
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D006834:Hydrazines / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009532:Nickel / Q000032:analysis* / Q000378:metabolism; D013890:Thiourea / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: C000597840:2-(2-fluorobenzoyl)-N-(2-methoxyphenyl)hydrazinecarbothioamide; D005456:Fluorescent Dyes; D006834:Hydrazines; D009532:Nickel; D013890:Thiourea