title: Antioxidant activity of carboxymethyl (1→3)-β-d-glucan (from the sclerotium of Poria cocos) sulfate (in vitro).
abstract: (1→3)-β-d-glucan derived from Poria cocos hardly exhibits bioactivities. To extend its use, three types of (1→3)-β-d-glucan derivatives, which were sulfated (1→3)-β-d-glucan (S-P), carboxymethyl (1→3)-β-d-glucan (CMP) and carboxylmethyl (1→3)-β-d-glucan sulfate (S-CMP), were synthesized. Potential antioxidant activities of S-P, CMP and S-CMP were evaluated in vitro. The experiments of scavenging abilities of free radicals were carried out, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion and hydroxyl. Deeply study of the derivatives' inhibitory effect for lipid peroxidation, DNA oxidative damage, erythrocyte hemolysis, and malondialdehyde (MDA) production were determined. And S-CMP significantly (P<0.01) increased the antioxidant activity of β-glucan. These results showed that multiple modifications of polysaccharides may bring the derivatives with excellent properties and various applications.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152#, Luoyu Road, Wuhan, Hubei 430079, PR China. Electronic address: wqbetter@126.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152#, Luoyu Road, Wuhan, Hubei 430079, PR China.
authors.forename: Sha
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152#, Luoyu Road, Wuhan, Hubei 430079, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Han
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152#, Luoyu Road, Wuhan, Hubei 430079, PR China.
authors.forename: Mengting
authors.initials: M
authors.lastname: Lian
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152#, Luoyu Road, Wuhan, Hubei 430079, PR China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Wen
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152#, Luoyu Road, Wuhan, Hubei 430079, PR China.
authors.forename: Telieke
authors.initials: T
authors.lastname: Jiayinaguli
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152#, Luoyu Road, Wuhan, Hubei 430079, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152#, Luoyu Road, Wuhan, Hubei 430079, PR China.
authors.forename: Renqiang
authors.initials: R
authors.lastname: Sun
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152#, Luoyu Road, Wuhan, Hubei 430079, PR China. Electronic address: caoyu@mail.ccnu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D004249:DNA Damage; D004912:Erythrocytes / Q000187:drug effects; D005609:Free Radicals / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008930:Mitochondria, Liver / Q000187:drug effects; D025282:Mycelium / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D055441:Poria / Q000737:chemistry*; D051381:Rats; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000737:chemistry*; D047071:beta-Glucans / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D013431:Sulfates; D047071:beta-Glucans; D008315:Malondialdehyde; C029933:carboxymethyl-beta-1,3-glucan; D006861:Hydrogen Peroxide
keywords: Antioxidant activities; Carboxymethyl (1→3)-β-d-glucan sulfate; Poria cocos; Synergy effect