title: Bioleaching characteristics, influencing factors of Cu solubilization and survival of Herbaspirillum sp. GW103 in Cu contaminated mine soil.
abstract: This study was aimed at assess the potential of diazotrophic bacteria, Herbaspirillum sp. GW103, for bioleaching of Cu in mine soil. The strain exhibited resistance to As (550mgL(-1)), Cu (350mgL(-1)), Zn (300mgL(-1)) and Pb (200mgL(-1)). The copper resistance was further confirmed by locating copA and copB genes. The survival of the isolate GW103 during bioleaching was analyzed using green fluorescent protein tagged GW103. Response surface methodology based Box-Behnken design was used to optimize the physical and chemical conditions for Cu bioleaching. Five significant variables (temperature, incubation time, CaCO3, coconut oil cake (COC), agitation rate) were selected for the optimization. Second-order polynomials were established to identify the relationship between Cu bioleaching and variables. The optimal conditions for maximum Cu bioleaching (66%) were 30°C, 60h of incubation with 1.75% of CaCO3 and 3% COC at 140rpm. The results of Cu sequential extraction studies indicated that the isolate GW103 leached Cu from ion-exchangeable, reducible, strong organic and residual fractions. Obtained results point out that the isolate GW103 could be used for bioleaching of Cu from mine soils.
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, South Korea.
authors.forename: Muthusamy
authors.initials: M
authors.lastname: Govarthanan
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, South Korea.
authors.forename: Gun-Woong
authors.initials: GW
authors.lastname: Lee
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, South Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 561-756, South Korea.
authors.forename: Jae Su
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Korea Rural Community Corp., Uiwang-si, Gyeonggi-do 437-703, South Korea.
authors.forename: Sung-Sik
authors.initials: SS
authors.lastname: Lim
authors.affiliation: Korea Rural Community Corp., Uiwang-si, Gyeonggi-do 437-703, South Korea.
authors.forename: Sang-Ki
authors.initials: SK
authors.lastname: Seo
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, South Korea.
authors.forename: Min
authors.initials: M
authors.lastname: Cho
authors.affiliation: Department of Ecology Landscape Architecture-Design, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, South Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Myung
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, South Korea. Electronic address: kannan@jbnu.ac.kr.
authors.forename: Seralathan
authors.initials: S
authors.lastname: Kamala-Kannan
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, South Korea. Electronic address: btoh@jbnu.ac.kr.
authors.forename: Byung-Taek
authors.initials: BT
authors.lastname: Oh
mesh_terms: D002119:Calcium Carbonate / Q000737:chemistry; D000074263:Coconut Oil; D003300:Copper / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D042803:Herbaspirillum / Q000187:drug effects / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D012995:Solubility; D013696:Temperature; D013997:Time Factors
chemical_list: D010938:Plant Oils; D012987:Soil; D012989:Soil Pollutants; D003300:Copper; D002119:Calcium Carbonate; D000074263:Coconut Oil
keywords: Bioleaching; Green fluorescent protein; Herbaspirillum sp.; Mine soil; Oil cake