title: Propolis Reduces Phosphatidylcholine-Specific Phospholipase C Activity and Increases Annexin a7 Level in Oxidized-LDL-Stimulated Human Umbilical Vein Endothelial Cells.
abstract: To understand the mechanisms underlying the regulating dyslipidemia action of Chinese propolis and Brazilian green propolis, we investigated their effects on phosphatidylcholine-specific phospholipase C (PC-PLC) activity and annexin a7 (ANXA7) level which play crucial roles in the control of the progress of atherosclerosis. Furthermore, active oxygen species (ROS) levels, nuclear factor-KappaB p65 (NF- κ B p65), and mitochondrial membrane potential (MMP) were also investigated in oxidized-LDL- (ox-LDL-) stimulated human umbilical vein endothelial cells (HUVECs). Our data indicated that the treatment of both types of propolis 12.5  μ g/mL significantly increased cell viability and attenuated apoptosis rate, increased ANXA7 level, and decreased PC-PLC activity. Both types of propolis also inhibited ROS generation as well as the subsequent MMP collapse, and NF- κ B p65 activation induced by ox-LDL in HUVECs. Our results also indicated that Chinese propolis and Brazilian green propolis had similar biological activities and prevented ox-LDL induced cellular dysfunction in HUVECs.
authors.affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, China ; College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.
authors.forename: Hongzhuan
authors.initials: H
authors.lastname: Xuan
authors.affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
authors.forename: Jiying
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.
authors.forename: Kai
authors.identifier: 0000-0003-0553-5293
authors.initials: K
authors.lastname: Wang
authors.affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, China.
authors.forename: Chongluo
authors.initials: C
authors.lastname: Fu
authors.affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Yuan
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.
authors.forename: Fuliang
authors.initials: F
authors.lastname: Hu