title: Banded structures in collagen vitrigels for corneal injury repair.
abstract: There is a growing interest in using collagen vitrigels for corneal injury repair. We recently reported the synthesis and thermal denaturation behavior of these gels. In this paper, the banded structure in these vitrified gels is studied by small-angle X-ray scattering (SAXS) one-dimensional (1-D) correlation function analysis and transmission electron microscopy (TEM). Results demonstrate that the collagen vitrigel possess banded structures similar to those of the starting type I collagen, with an average D-spacing of 64nm (by SAXS) or 57nm (by TEM). A combination of SAXS 1-D correlation function analyses and TEM show that overlap and gap distances ranged from 30 to 33nm and from 23 to 25nm, respectively. Changing the vitrification condition does not impact on the banded structure significantly.
authors.affiliation: The Johns Hopkins University, Applied Physics Laboratory, Laurel, MD 20723, USA. Electronic address: zhiyong.xia@jhuapl.edu.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Xia
authors.affiliation: The Johns Hopkins University, Applied Physics Laboratory, Laurel, MD 20723, USA.
authors.forename: Xiomara
authors.initials: X
authors.lastname: Calderón-Colón
authors.affiliation: The Johns Hopkins University, Applied Physics Laboratory, Laurel, MD 20723, USA; The Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
authors.forename: Russell
authors.initials: R
authors.lastname: McCally
authors.affiliation: The Johns Hopkins University, Applied Physics Laboratory, Laurel, MD 20723, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Maranchi
authors.affiliation: State University of New York at Stony Brook, Stony Brook, NY 11794-3400, USA.
authors.forename: Lixia
authors.initials: L
authors.lastname: Rong
authors.affiliation: The Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Hsiao
authors.affiliation: The Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; The Johns Hopkins University, Department of Biomedical Engineering, Baltimore, MD 21231, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Elisseeff
authors.affiliation: The Johns Hopkins University, Applied Physics Laboratory, Laurel, MD 20723, USA. Electronic address: morgana.trexler@jhuapl.edu.
authors.forename: Morgana
authors.initials: M
authors.lastname: Trexler
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D065306:Corneal Injuries / Q000628:therapy*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D008853:Microscopy; D046529:Microscopy, Electron, Transmission; D008968:Molecular Conformation; D053838:Scattering, Small Angle; D054457:Tissue Scaffolds; D058989:Vitrification; D014961:X-Ray Diffraction
chemical_list: D020100:Hydrogels; D003094:Collagen
keywords: Banded structure; Corneal injury repair; D-Spacing; Small-angle X-ray scattering; collagen vitrigel