title: Mannose-binding lectin gene polymorphism and risk factors for cardiovascular disease in postmenopausal women.
abstract: BACKGROUND
  Inflammatory responses may be altered in postmenopausal women and predispose to cardiovascular disease (CVD). Genetic factors can also influence susceptibility to CVD. Mannose-binding lectin (MBL) is a component of the innate immune system and an activator of the complement cascade. We evaluated the association of genetic polymorphism of MBL (MBL2) on risk factors for CVD in postmenopausal women.
  
  
  METHODS
  In this cross-sectional study, 311 Brazilian women (age ≥45 years and amenorrhea ≥12 months) were included.
  
  
  METHODS
  presence of previous or current CVD, insulin dependent diabetes, chronic kidney disease, autoimmune diseases and cancer. Clinical, anthropometric and biochemical assessments were performed to evaluate the cardiovascular risk factors. DNA was extracted from buccal cell and polymorphisms at codons 54 and 57 in the MBL2 were determined by polymerase chain reaction (PCR). For statistical analysis, the chi-square and logistic regression (odds ratio, OR) were used.
  
  
  RESULTS
  The presence of the polymorphic allele for codon 54 was found in 25.8% of women (A/B=22.6%, B/B=3.2%) and for codon 57 in 12.2% (A/C=10.8%, C/C=1.4%). The polymorphism at codon 54 was significantly associated with the presence of hypertension (OR 0.55, 95% CI 0.31-0.99, p=0.044) and insulin resistance assessed by HOMA-IR (OR 0.46, 95% CI 0.24-0.91, p=0.025). No significant associations were observed between the polymorphism at codon 57 with risk factors for CVD.
  
  
  CONCLUSIONS
  In postmenopausal women, the polymorphism at codon 54 of the MBL2 was associated with lower risk for hypertension and insulin resistance that are important risk factors for CVD.
authors.affiliation: Department of Gynecology and Obstetrics, Botucatu Medical School, Sao Paulo State University- UNESP, Botucatu, São Paulo, Brazil. Electronic address: claudiorsatti@gmail.com.
authors.forename: Claudio Lera
authors.initials: CL
authors.lastname: Orsatti
authors.affiliation: Department of Gynecology and Obstetrics, Botucatu Medical School, Sao Paulo State University- UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Eliana Aguiar Petri
authors.initials: EA
authors.lastname: Nahás
authors.affiliation: Department of Gynecology and Obstetrics, Botucatu Medical School, Sao Paulo State University- UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Jorge
authors.initials: J
authors.lastname: Nahas-Neto
authors.affiliation: Department of Sports Science, Institute of Health Sciences - UFTM, Uberaba, Minas Gerais, Brazil.
authors.forename: Fabio Lera
authors.initials: FL
authors.lastname: Orsatti
authors.affiliation: Department of Gynecology and Obstetrics, University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Iara Moreno
authors.initials: IM
authors.lastname: Linhares
authors.affiliation: Department of Obstetrics and Gynecology, Division of Immunology and Infectious Diseases, Weill Cornell Medical College, New York, NY, USA.
authors.forename: Steven Sol
authors.initials: SS
authors.lastname: Witkin
mesh_terms: D000368:Aged; D002318:Cardiovascular Diseases / Q000235:genetics*; D016009:Chi-Square Distribution; D003062:Codon / Q000235:genetics; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000235:genetics; D007333:Insulin Resistance / Q000235:genetics; D016015:Logistic Models; D037601:Mannose-Binding Lectin / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D017698:Postmenopause; D012307:Risk Factors
chemical_list: D003062:Codon; C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin
keywords: Cardiovascular disease; Mannose-binding lectin; Polymorphism; Postmenopausal women