title: Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2.
abstract: The present study evaluated the effect of nasally given Lactobacillus rhamnosus CRL1505 on the immunocoagulative response during pneumococcal infection in immunocompetent mice. In addition, we aimed to gain insight into the mechanism involved in the immunomodulatory effect of the L. rhamnosus CRL1505 strain by evaluating the role of TLR2. Results showed that nasally given L. rhamnosus CRL1505 effectively regulates inflammation and hemostatic alterations during the pneumococcal infection. Immunobiotic treatment significantly reduced permeability of the bronchoalveolar-capillary barrier, and general cytotoxicity, decreasing lung tissue damage. The CRL1505 strain improved the production of TNF-α, IFN-γ, and IL-10 after pneumococcal challenge. In addition, increased TM and TF expressions were found in lungs of L. rhamnosus CRL1505-treated mice. Moreover, we demonstrated, for the first time, that the TLR2 signaling pathway has a role in the induction of IFN-γ and IL-10 and in the reduction of TF. The results also allow us to speculate that a PRR, other than TLR2, may mediate the immunobiotic activity of L. rhamnosus CRL1505 and could explain changes in TNF-α and TM.
authors.affiliation: Immunobiotics Research Group, Tucuman; Applied Biochemistry Institute, Faculty of Biochemistry, Chemistry and Pharmacy, Tucuman University.
authors.forename: Hortensia
authors.initials: H
authors.lastname: Zelaya
authors.forename: Julio
authors.initials: J
authors.lastname: Villena
authors.forename: Andres Gramajo
authors.initials: AG
authors.lastname: Lopez
authors.forename: Susana
authors.initials: S
authors.lastname: Alvarez
authors.forename: Graciela
authors.initials: G
authors.lastname: Agüero
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D056747:Immunomodulation; D052201:Lacticaseibacillus rhamnosus / Q000276:immunology*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D011018:Pneumonia, Pneumococcal / Q000097:blood / Q000276:immunology* / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
chemical_list: D016207:Cytokines; D051195:Toll-Like Receptor 2
keywords: Lactobacillus rhamnosus CRL1505; Toll-like receptor 2; hemostasis-inflammation; lung injury