title: A low detection limit penicillin biosensor based on single graphene nanosheets preadsorbed with hematein/ionic liquids/penicillinase.
abstract: In this study, we reported on a low detection limit penicillin biosensor with layer-by-layer (LbL) film containing single-graphene nanosheets (SGNs) preadsorbed with hematein, ionic liquids (ILs) and penicillinase. The penicillinase catalyzes the hydrolysis of penicillin to penicilloic acid, where H(+) is liberated and monitored amperometrically with hematein as a pH indicator. The SGN-hematein/ILs/penicillinase biosensor exhibited excellent performance for penicillin in PBS with a wide range from 1.25×10(-13) to 7.5×10(-3)M, and a low detection limit of 10(-13)M (0.04ppt, S/N≥3). Furthermore, the detection of penicillin concentration in real sample (milk) had acceptable accuracy with the assay system.
authors.affiliation: Fujian Key Lab of Medical Instrument & Pharmaceutical Technology, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; Institute of Research for Functional Materials, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; College of Chemistry and Chemical Engineering, Qishan Campus, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Yueting
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Fujian Key Lab of Medical Instrument & Pharmaceutical Technology, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; Institute of Research for Functional Materials, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; College of Chemistry and Chemical Engineering, Qishan Campus, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Lele
authors.initials: L
authors.lastname: Tang
authors.affiliation: Fujian Key Lab of Medical Instrument & Pharmaceutical Technology, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; Institute of Research for Functional Materials, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; College of Chemistry and Chemical Engineering, Qishan Campus, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Linhong
authors.initials: L
authors.lastname: Huang
authors.affiliation: Institute of Research for Functional Materials, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; College of Chemistry and Chemical Engineering, Qishan Campus, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Han
authors.affiliation: College of Chemistry and Chemical Engineering, Qishan Campus, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Fujian Key Lab of Medical Instrument & Pharmaceutical Technology, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; Institute of Research for Functional Materials, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; College of Chemistry and Chemical Engineering, Qishan Campus, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address: hbpan@fzu.edu.cn.
authors.forename: Haibo
authors.initials: H
authors.lastname: Pan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006416:Hematoxylin / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D052578:Ionic Liquids / Q000737:chemistry*; D057230:Limit of Detection; D010397:Penicillanic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D010405:Penicillinase / Q000737:chemistry*; D010406:Penicillins / Q000032:analysis*
chemical_list: D052578:Ionic Liquids; D010406:Penicillins; C008782:penicilloic acid; D006108:Graphite; D010397:Penicillanic Acid; C007915:hematein; D010405:Penicillinase; D006416:Hematoxylin
keywords: Electrochemistry; Graphene nanosheets; Hematein; Ionic liquids; Penicillin biosensor