title: A preliminary report on the genetic variation in pointed gourd (Trichosanthes dioica Roxb.) as assessed by random amplified polymorphic DNA.
abstract: Pointed gourd (Trichosanthes dioica Roxb.) is an economically important cucurbit and is extensively propagated through vegetative means, viz vine and root cuttings. As the accessions are poorly characterized it is important at the beginning of a breeding programme to discriminate among available genotypes to establish the level of genetic diversity. The genetic diversity of 10 pointed gourd races, referred to as accessions was evaluated. DNA profiling was generated using 10 sequence independent RAPD markers. A total of 58 scorable loci were observed out of which 18 (31.03%) loci were considered polymorphic. Genetic diversity parameters [average and effective number of alleles, Shannon's index, percent polymorphism, Nei's gene diversity, polymorphic information content (PIC)] for RAPD along with UPGMA clustering based on Jaccard's coefficient were estimated. The UPGMA dendogram constructed based on RAPD analysis in 10 pointed gourd accessions were found to be grouped in a single cluster and may represent members of one heterotic group. RAPD analysis showed promise as an effective tool in estimating genetic polymorphism in different accessions of pointed gourd.
authors.affiliation: University of Kalyani Department of Botany Kalyani 741235 West Bengal India.
authors.forename: S
authors.initials: S
authors.lastname: Adhikari
authors.affiliation: University of Kalyani Department of Botany Kalyani 741235 West Bengal India.
authors.forename: A
authors.initials: A
authors.lastname: Biswas
authors.affiliation: University of Kalyani Department of Molecular Biology and Biotechnology Kalyani 741235 West Bengal India.
authors.forename: T K
authors.initials: TK
authors.lastname: Bandyopadhyay
authors.affiliation: University of Kalyani Department of Botany Kalyani 741235 West Bengal India.
authors.forename: P D
authors.initials: PD
authors.lastname: Ghosh
mesh_terms: D016000:Cluster Analysis; D018744:DNA, Plant / Q000235:genetics*; D056426:Genetic Loci; D005819:Genetic Markers; D005838:Genotype; D010641:Phenotype; D018515:Plant Leaves; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D029778:Trichosanthes / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: DNA marker; RAPD; Trichosanthes dioica; genetic diversity; pointed gourd