title: RNA-peptide conjugate synthesis by inverse-electron demand Diels-Alder reaction.
abstract: Here we report an efficient method for the synthesis of RNA-peptide conjugates by inverse-electron demand Diels-Alder reaction. Various dienophiles were enzymatically incorporated into RNA and reacted with a chemically synthesized diene-modified peptide. The Diels-Alder reaction proceeds with near-quantitative yields in aqueous solution with stoichiometric amounts of reactants, even at low micromolar concentrations.
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany. jaeschke@uni-hd.de.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Ameta
authors.forename: Juliane
authors.initials: J
authors.lastname: Becker
authors.forename: Andres
authors.initials: A
authors.lastname: Jäschke
mesh_terms: D002853:Chromatography, Liquid; D061565:Cycloaddition Reaction; D004583:Electrons; D004591:Electrophoresis, Polyacrylamide Gel; D013058:Mass Spectrometry; D009711:Nucleotides / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D009711:Nucleotides; D010455:Peptides; D012313:RNA