title: Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate.
abstract: Human pluripotent stem cells (hPSCs), including human embryonic stem cells and human-induced pluripotent stem cells, are a renewable cell source for a wide range of applications in regenerative medicine and useful tools for human disease modeling and drug discovery. For these purposes, large numbers of high-quality cells are essential. Recently, we showed that a biological substrate, recombinant E8 fragments of laminin isoforms, sustains long-term self-renewal of hPSCs in defined, xeno-free medium with dissociated single-cell passaging. Here, we describe a modified culture system with similar performance to efficiently expand hPSCs under defined, xeno-free conditions using a non-biological synthetic substrate.
authors.affiliation: Facility of Cell Processing, Department of Embryonic Stem Cell Research, Institute for Frontier Medical Sciences, Kyoto University, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan, kawase8@frontier.kyoto-u.ac.jp.
authors.forename: Eihachiro
authors.initials: E
authors.lastname: Kawase
mesh_terms: D000179:Acrylates / Q000494:pharmacology*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D015925:Cryopreservation; D003470:Culture Media / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D007621:Karyotyping; D010455:Peptides / Q000494:pharmacology*; D039904:Pluripotent Stem Cells / Q000166:cytology*
chemical_list: D000179:Acrylates; D003470:Culture Media; D010455:Peptides