title: Dibenzocyclooctadiene lignans from Schisandra chinensis and their inhibitory activity on NO production in lipopolysaccharide-activated microglia cells.
abstract: Four dibenzocyclooctadiene lignans, schisanchinins A-D, and 10 known compounds were isolated from the EtOAc extract of fruits of Schisandra chinensis (Turcz.) Baill. Structures of compounds 1-4 were elucidated using a combination of spectroscopic techniques, including MS, UV and IR, NMR ((1)H NMR, (13)C NMR, HMQC, HMBC). The stereochemistry of the chiral centers and the biphenyl configuration were determined using NOESY, as well as analysis of CD spectra. In vitro activity assays showed that 11 of the 14 compounds exhibited inhibitory activity on lipopolysaccharide (LPS)-induced NO release in primary murine BV2 microglia cells.
authors.affiliation: Development and Utilization Key Laboratory of Northeast Plant Materials of Liaoning Province, Department of Pharmacognosy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Di
authors.initials: D
authors.lastname: Hu
authors.affiliation: Development and Utilization Key Laboratory of Northeast Plant Materials of Liaoning Province, Department of Pharmacognosy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Xuechun
authors.initials: X
authors.lastname: Yao
authors.affiliation: Development and Utilization Key Laboratory of Northeast Plant Materials of Liaoning Province, Department of Pharmacognosy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Development and Utilization Key Laboratory of Northeast Plant Materials of Liaoning Province, Department of Pharmacognosy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Na
authors.initials: N
authors.lastname: Han
authors.affiliation: Development and Utilization Key Laboratory of Northeast Plant Materials of Liaoning Province, Department of Pharmacognosy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The Chinese People's Liberation Army 463 Hospital, Shenyang 110042, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Yang
authors.affiliation: Development and Utilization Key Laboratory of Northeast Plant Materials of Liaoning Province, Department of Pharmacognosy, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: yinjun2002@yahoo.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D034242:Cyclooctanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice; D017628:Microglia / Q000187:drug effects; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D032141:Schisandra / Q000737:chemistry*
chemical_list: D034242:Cyclooctanes; D017705:Lignans; D008070:Lipopolysaccharides; D009573:Nitrites; D010936:Plant Extracts; C510994:dibenzocyclooctadiene lignan; D009569:Nitric Oxide
keywords: Dibenzocyclooctadiene lignans; Inhibitory activity on lipopolysaccharide (LPS)-induced NO release; Schisandra chinensis; Schisandraceae