title: Staurosporine induced apoptosis rapidly downregulates TDP- 43 in glioma cells.
abstract: TDP-43 is a ubiquitously expressed DNA/RNA binding protein that has recently attracted attention for its involvement in neurodegenerative diseases. While TDP-43 has been found to participate in various important cellular activities including stress and apoptosis, little is known about its role in cancer cells. Here we report that staurosporine (STS) induced apoptosis in U87 glioma cells is associated with rapid downregulation of TDP-43 at both mRNA and protein levels. The latter is dependent on activation of caspase 3. More importantly, we have shown that knockdown of TDP-43 by specific siRNA dramatically enhanced cytotoxicity of STS. These results suggest that normal level of TDP-43 may be protective for cancer cells under apoptotic insult.
authors.affiliation: School of Preclinical Medicine, Center Laboratory, Beijing University of Chinese Medicine, Beijing, China E-mail : huaq@bucm.edu.cn; w.jia@ubc.ca.
authors.forename: Yi-Nan
authors.initials: YN
authors.lastname: Nan
authors.forename: Jing-Yan
authors.initials: JY
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Tan
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: William
authors.initials: W
authors.lastname: Jia
authors.forename: Qian
authors.initials: Q
authors.lastname: Hua
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000187:drug effects*; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005910:Glioma; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects*; D019311:Staurosporine / Q000494:pharmacology*
chemical_list: D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D053148:Caspase 3; D019311:Staurosporine