title: The effect of pomelo mix ethyl acetate extract on CYP3A6 and P-glycoprotein gene transcripts in rabbits.
abstract: Pomelo fruit juice and pomelo ethylacetate extract have been shown to increase the bioavailability of some CYP3A substrates. The purpose of this study was to investigate if this effect might be contributed to by changes in CYP3A and p-glycoprotein mRNAs levels in the liver and proximal small intestine. The ethyl acetate extract of pomelo mix was administered for 7 days to 10 rabbits. Nine rabbits were administered tap water for 7 days. The administration was through oral intubation to the stomach. On the 8(th) day, the rabbits were sacrificed, and the liver and the proximal 15 cm of the small intestine were dissected. Total RNA was extracted from the specimens and cDNA was prepared by quantitative real-time-polymerase chain reaction (RT-PCR) using specific primers. The ethyl acetate extract of pomelo mix reduced the mRNA expression of CYP3A6 almost 5-folds in the intestine and 2-folds in the liver. In contrast, a 1-fold increase to the p-glycoprotein mRNA expression was observed under the same experimental conditions. In conclusion, the ethyl acetate extract of pomelo mix reduced the mRNA expression of CYP3A6 in both intestine and liver but to different degrees, while the p-glycoprotein mRNA expression was not reduced.
authors.forename: Yacoub M
authors.initials: YM
authors.lastname: Irshaid
authors.forename: Malek A
authors.initials: MA
authors.lastname: Zihlef
authors.forename: Suheil M
authors.initials: SM
authors.lastname: Zmeili
authors.forename: Eman T
authors.initials: ET
authors.lastname: Al-Antary
authors.forename: Mais G
authors.initials: MG
authors.lastname: Zmaily
authors.forename: Somya N
authors.initials: SN
authors.lastname: Al-Embideen
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Jordan, Amman 11942, Jordan. y.irshaid@ju.edu.jo.
authors.forename: Abdallah O
authors.initials: AO
authors.lastname: Amireh
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D000085:Acetates / Q000737:chemistry*; D000284:Administration, Oral; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D002957:Citrus / Q000737:chemistry; D015536:Down-Regulation; D005638:Fruit; D007421:Intestine, Small / Q000187:drug effects* / Q000201:enzymology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000085:Acetates; D010936:Plant Extracts; D012333:RNA, Messenger; D012997:Solvents; C007650:ethyl acetate; D001189:Aryl Hydrocarbon Hydroxylases; C100638:cytochrome P-450 CYP3A6 (rabbit)