title: Identification and catalytic characterization of a nonribosomal peptide synthetase-like (NRPS-like) enzyme involved in the biosynthesis of echosides from Streptomyces sp. LZ35.
abstract: Echosides, isolated from Streptomyces sp. LZ35, represent a class of para-terphenyl natural products that display DNA topoisomerase I and IIα inhibitory activities. By analyzing the genome draft of strain LZ35, the ech gene cluster was identified to be responsible for the biosynthesis of echosides, which was further confirmed by gene disruption and HPLC analysis. Meanwhile, the biosynthetic pathway for echosides was proposed. Furthermore, the echA-gene, encoding a tri-domain nonribosomal peptide synthetase (NRPS)-like enzyme, was identified as a polyporic acid synthetase and biochemically characterized in vitro. This is the first study to our knowledge on the biochemical characterization of an Actinobacteria quinone synthetase, which accepts phenylpyruvic acid as a native substrate. Therefore, our results may help investigate the function of other NRPS-like enzymes in Actinobacteria.
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, PR China.
authors.forename: Wang
authors.initials: W
authors.lastname: Chen
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, PR China.
authors.forename: Yao-Yao
authors.initials: YY
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, PR China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: De-Yu
authors.initials: DY
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Guo-Yin
authors.initials: GY
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, PR China. Electronic address: wanghaoxin@sdu.edu.cn.
authors.forename: Hao-Xin
authors.initials: HX
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, PR China; Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, PR China. Electronic address: yshen@sdu.edu.cn.
authors.forename: Yue-Mao
authors.initials: YM
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics; D013730:Terphenyl Compounds / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D013730:Terphenyl Compounds; D010453:Peptide Synthases
keywords: Biosynthesis; Echosides; Nonribosomal peptide synthetase; Quinone synthetase; Streptomyces sp.