title: Sucrose favors Pseudomonas aeruginosa pellicle production through the extracytoplasmic function sigma factor SigX.
abstract: Pseudomonas aeruginosa biofilm formation was increased by addition of sucrose to Luria-Bertani medium, whereas addition of NaCl to a final similar osmolarity and use of maltose instead of sucrose, were ineffective. In a previous study, we showed that the extracytoplasmic sigma factor SigX is activated in the presence of sucrose. The sucrose-mediated pellicle increase was abolished in a sigX mutant strain. Sucrose addition led to an increase in pel expression and cyclic-diguanylate (c-di-GMP) pool level production. Interestingly, these two phenotypes were strongly decreased in a sigX mutant. Since pel is not known as a SigX-target, we suspect SigX to be involved in the c-di-GMP production. We found that expression of the diguanylate cyclase PA4843 gene was increased in the presence of sucrose at least partly through SigX activity. Our study shows that sucrose itself rather than osmolarity favours the biofilm mode of P. aeruginosa through the activation of SigX.
authors.affiliation: Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM), EA 4312, Normandie Université, Université de Rouen, Rouen, France.
authors.forename: Emeline
authors.initials: E
authors.lastname: Bouffartigues
authors.forename: Rachel
authors.initials: R
authors.lastname: Duchesne
authors.forename: Alexis
authors.initials: A
authors.lastname: Bazire
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Simon
authors.forename: Olivier
authors.initials: O
authors.lastname: Maillot
authors.forename: Alain
authors.initials: A
authors.lastname: Dufour
authors.forename: Marc
authors.initials: M
authors.lastname: Feuilloley
authors.forename: Nicole
authors.initials: N
authors.lastname: Orange
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chevalier
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis; D017353:Gene Deletion; D015870:Gene Expression; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D019761:Phosphorus-Oxygen Lyases / Q000096:biosynthesis; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*
chemical_list: D003470:Culture Media; D029968:Escherichia coli Proteins; D012808:Sigma Factor; D012965:Sodium Chloride; D013395:Sucrose; C062025:bis(3',5')-cyclic diguanylic acid; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase; D006152:Cyclic GMP
keywords: Pel; SigX; c-di-GMP; pellicle; sucrose