title: Small molecules differentiate definitive endoderm from human induced pluripotent stem cells on PCL scaffold.
abstract: Human induced pluripotent stem cells (hiPSCs) are attractive sources of cells for disease modeling in vitro, and they may eventually provide access to cells/tissues for the treatment of many degenerative diseases. Stepwise differentiation from hiPSCs to definitive endoderm (DE) will identify a key step in hepatocytes and beta cell development and may prove useful for transplantation therapy for liver diseases and diabetes. Inducer of definitive endoderm 1 (IDE1) is known to play an important role in the regional specification of DE. Here, we have investigated the effect of stimulation with IDE1 on the development of hiPSCs into DE cells in three-dimensional (3D) cultures. The differentiation was determined by immunofluorescence staining with Sox17, FoxA2, and goosecoid (Gsc) and also by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis. In this study, we showed that hiPSCs with 6-day IDE1 treatment (as chemical tool) on poly(ε-caprolactone) (PCL) nanofibrous scaffold were able to differentiate into DE cells.
authors.affiliation: Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran, e.hoveizi@yahoo.com.
authors.forename: Elham
authors.initials: E
authors.lastname: Hoveizi
authors.forename: Sirus
authors.initials: S
authors.lastname: Khodadadi
authors.forename: Shima
authors.initials: S
authors.lastname: Tavakol
authors.forename: Oveis
authors.initials: O
authors.lastname: Karima
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Nasiri-Khalili
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004707:Endoderm / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects*; D057139:Nanofibers / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D011091:Polyesters; C016240:polycaprolactone