title: Magnetic assembly-mediated enhancement of differentiation of mouse bone marrow cells cultured on magnetic colloidal assemblies.
abstract: Here we reported an interesting phenomenon that the field-induced assemblies of magnetic nanoparticles can promote the differentiation of primary mouse bone marrow cells into osteoblasts. The reason was thought to lie in the remnant magnetic interaction inside the assemblies which resulted from the magnetic field-directed assembly. Influence of the assemblies on the cells was realized by means of interface effect rather than the internalization effect. We fabricated a stripe-like assemblies array on the glass plate and cultured cells on this surface. We characterized the morphology of assemblies and measured the mechanic property as well as the magnetic property. The cellular differentiation was measured by staining and quantitative PCR. Finally, Fe uptake was excluded as the reason to cause the phenomenon.
authors.affiliation: 1] State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Dingjiaqiao 87, Nanjing, P. R. China, 210009 [2].
authors.forename: Jianfei
authors.initials: J
authors.lastname: Sun
authors.affiliation: 1] School of Medicine, Southeast University, Dingjiaqiao 87, Nanjing, P. R. China, 210009 [2].
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Medicine, Southeast University, Dingjiaqiao 87, Nanjing, P. R. China, 210009.
authors.forename: Jiqing
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Dingjiaqiao 87, Nanjing, P. R. China, 210009.
authors.forename: Lina
authors.initials: L
authors.lastname: Song
authors.affiliation: School of Medicine, Southeast University, Dingjiaqiao 87, Nanjing, P. R. China, 210009.
authors.forename: Zihao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: School of Medicine, Southeast University, Dingjiaqiao 87, Nanjing, P. R. China, 210009.
authors.forename: Haoyu
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Dingjiaqiao 87, Nanjing, P. R. China, 210009.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Dingjiaqiao 87, Nanjing, P. R. China, 210009.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Dingjiaqiao 87, Nanjing, P. R. China, 210009.
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology* / Q000528:radiation effects; D002454:Cell Differentiation / Q000502:physiology* / Q000528:radiation effects; D002478:Cells, Cultured; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects; D010012:Osteogenesis / Q000502:physiology / Q000528:radiation effects
chemical_list: D058185:Magnetite Nanoparticles