title: Detection, epidemiology and characterization of VP6 and VP7 genes of group D rotavirus in broiler chickens.
abstract: Rotaviruses infect humans and animals and are classified into eight groups (A to H). Group D rotavirus (RVD) has been described in birds, although relatively few reports are available. The present study focused on RVD, including epidemiological and molecular aspects of samples collected from broiler chickens in the state of Pará, Brazil. A total of 85 faecal samples were collected between 2008 and 2011 from 37 chicken farms located in eight different municipalities. The viral double-stranded RNA was extracted from faecal suspensions and analysed using polyacrylamide gel electrophoresis (PAGE), followed by reverse transcriptase-polymerase chain reaction (RT-PCR) and nucleotide sequencing of the VP6 and VP7 genes. Comparing the positive results, 16.5% (14/85) were obtained by PAGE and 35.3% (30/85) by RT-PCR. Samples from seven of eight municipalities were positive for RVD and infections were recorded in 17 (45.9%) of 37 chicken farms. The RVD infection rate was significantly higher in the 16-day to 30-day age group (62.2%; 23/37) compared with other ages. No consistent relationship was found between the infection rate and either the population density in poultry houses or the climatic conditions. The nucleotide sequences of the VP6 gene were 89.9 to 90.9% similar to the prototype strain 05V0049 and were 88.3 to 100% similar among themselves; VP7 gene nucleotide sequences were 84.3 to 85.4% similar to the prototype strain 05V0049 and 93.8 to 100% similar among themselves. Overall, this study provides new insights into the epidemiology and genome characterization of group D rotaviruses.
authors.affiliation: a Seção de Virologia , Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde , Ananindeua , Brazil.
authors.forename: Delana Andreza Melo
authors.initials: DA
authors.lastname: Bezerra
authors.forename: René Ribeiro
authors.initials: RR
authors.lastname: da Silva
authors.forename: Jane Haruko Lima
authors.initials: JH
authors.lastname: Kaiano
authors.forename: Darleise
authors.initials: D
authors.lastname: de Souza Oliveira
authors.forename: Yvone Benchimol
authors.initials: YB
authors.lastname: Gabbay
authors.forename: Alexandre Costa
authors.initials: AC
authors.lastname: Linhares
authors.forename: Joana D'Arc Pereira
authors.initials: JD
authors.lastname: Mascarenhas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D036022:Capsid Proteins / Q000235:genetics*; D002645:Chickens / Q000821:virology*; D005243:Feces / Q000821:virology; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus