title: Effects of jacalin and follicle-stimulating hormone on in vitro goat primordial follicle activation, survival and gene expression.
abstract: This study aims to investigate the effects of jacalin and follicle-stimulating hormone (FSH) on activation and survival of goat primordial follicles, as well as on gene expression in cultured ovarian tissue. Ovarian fragments were cultured for 6 days in minimum essential medium (MEM) supplemented with jacalin (10, 25, 50 or 100 μg/ml - Experiment 1) or in MEM supplemented with jacalin (50 μg/ml), FSH (50 ng/ml) or both (Experiment 2). Non-cultured and cultured tissues were processed for histological and ultrastructural analysis. Cultured tissues from Experiment 2 were also stored to evaluate the expression of BMP-15, KL (Kit ligand), c-kit, GDF-9 and proliferating cell nuclear antigen (PCNA) by real-time polymerase chain reaction (PCR). The results of Experiment 1 showed that, compared with tissue that was cultured in control medium, the presence of 50 μg/ml of jacalin increased both the percentages of developing follicles and viability. In Experiment 2, after 6 days, higher percentages of normal follicles were observed in tissue cultured in presence of FSH, jacalin or both, but no synergistic interaction between FSH and jacalin was observed. These substances had no significant effect on the levels of mRNA for BMP-15 and KL, but FSH increased significantly the levels of mRNA for PCNA and c-kit. On the other hand, jacalin reduced the levels of mRNA for GDF-9. In conclusion, jacalin and FSH are able to improve primordial follicle activation and survival after 6 days of culture. Furthermore, presence of FSH increases the expression of mRNA for PCNA and c-kit, but jacalin resulted in lower GDF-9 mRNA expression.
authors.affiliation: Biotechnology Nucleus of Sobral-NUBIS,Federal University of Ceara,CEP 62042-280,Sobral,CE,Brazil.
authors.forename: Regislane P
authors.initials: RP
authors.lastname: Ribeiro
authors.affiliation: Biotechnology Nucleus of Sobral-NUBIS,Federal University of Ceara,CEP 62042-280,Sobral,CE,Brazil.
authors.forename: Antonia M L R
authors.initials: AM
authors.lastname: Portela
authors.affiliation: Biotechnology Nucleus of Sobral-NUBIS,Federal University of Ceara,CEP 62042-280,Sobral,CE,Brazil.
authors.forename: Anderson W B
authors.initials: AW
authors.lastname: Silva
authors.affiliation: Biotechnology Nucleus of Sobral-NUBIS,Federal University of Ceara,CEP 62042-280,Sobral,CE,Brazil.
authors.forename: José J N
authors.initials: JJ
authors.lastname: Costa
authors.affiliation: Biotechnology Nucleus of Sobral-NUBIS,Federal University of Ceara,CEP 62042-280,Sobral,CE,Brazil.
authors.forename: José R S
authors.initials: JR
authors.lastname: Passos
authors.affiliation: Biotechnology Nucleus of Sobral-NUBIS,Federal University of Ceara,CEP 62042-280,Sobral,CE,Brazil.
authors.forename: Ellen V
authors.initials: EV
authors.lastname: Cunha
authors.affiliation: Biotechnology Nucleus of Sobral-NUBIS,Federal University of Ceara,CEP 62042-280,Sobral,CE,Brazil.
authors.forename: Glaucinete B
authors.initials: GB
authors.lastname: Souza
authors.affiliation: Biotechnology Nucleus of Sobral-NUBIS,Federal University of Ceara,CEP 62042-280,Sobral,CE,Brazil.
authors.forename: Márcia V A
authors.initials: MV
authors.lastname: Saraiva
authors.affiliation: Laboratory of Ultrastructure,CPqAM/Fiocruz,Federal University of Pernambuco,Recife,PE,Brazil.
authors.forename: Mariana A M
authors.initials: MA
authors.lastname: Donato
authors.affiliation: Laboratory of Ultrastructure,CPqAM/Fiocruz,Federal University of Pernambuco,Recife,PE,Brazil.
authors.forename: Christina A
authors.initials: CA
authors.lastname: Peixoto
authors.affiliation: Department of Pathobiology,Faculty of Veterinary Medicine,Utrecht University,PO Box 80.163,Utrecht,The Netherlands.
authors.forename: Robert
authors.initials: R
authors.lastname: van den Hurk
authors.affiliation: Biotechnology Nucleus of Sobral - NUBIS,Federal University of Ceara,Av. Comandante Maurocélio Rocha Ponte 100,CEP 62041-040,Sobral,CE,Brazil.
authors.forename: José R V
authors.initials: JR
authors.lastname: Silva
mesh_terms: D000818:Animals; D055430:Bone Morphogenetic Protein 15 / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006041:Goats; D055429:Growth Differentiation Factor 9 / Q000235:genetics; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D037121:Plant Lectins / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D019089:Stem Cell Factor / Q000235:genetics; D046509:Tissue Culture Techniques
chemical_list: D055430:Bone Morphogenetic Protein 15; D055429:Growth Differentiation Factor 9; D037121:Plant Lectins; D018809:Proliferating Cell Nuclear Antigen; D019089:Stem Cell Factor; C043696:jacalin; D005640:Follicle Stimulating Hormone
keywords: Caprine; Culture; Jacalin; Ovarian fragments; Pre-antral follicles; mRNA