title: Effects of postmortem interval on mouse ovary oocyte survival and maturation.
abstract: To study the time- and temperature-dependent survival of ovarian oocytes collected from postmortem carcass, ICR mice were killed and placed for different periods (0, 1, 2, 4, 6, 8 and 10 h) at different temperatures (25°C, 4°C and 37°C). After preservation, oocyte morphology, germinal vesicle (GV) oocyte number, oocyte meiotic maturation percentage, mitochondrial distribution and intracellular glutathione (GSH) level were evaluated. The results showed no surviving oocytes could be collected by 2h, 6h, and 12 h after carcass preservation at 37°C, 25°C and 4°C, respectively. The number of collected GV oocytes in the ovary deceased as the preservation time lasted at the same temperature. Meanwhile at the same point in time, the ratio of germinal vesicle breakdown (GVBD) and the first polar body emission (PBE) gradually reduced as preservation temperature increased. In addition, the percentage of abnormal mitochondrial distribution in the preserved oocytes was obviously higher than that in the control oocytes, while GSH level was not altered in collected oocytes. Unexpectedly, neither chromosome arrangement nor spindle organization was affected as long as the oocytes from preserved carcasses could complete maturation. These data are helpful for proper use of ovary oocytes from postmortem carcass of valuable individuals.
authors.affiliation: Diabetes research Center of Beijing University of Chinese Medicine, Beijing, China; Department of Biochemistry and Molecular Biology, Hebei United University, Tangshan, China.
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Zhang
authors.affiliation: Department of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China; State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jun-Yu
authors.initials: JY
authors.lastname: Ma
authors.affiliation: Diabetes research Center of Beijing University of Chinese Medicine, Beijing, China; Department of Biochemistry and Molecular Biology, Hebei United University, Tangshan, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Meng-Wen
authors.initials: MW
authors.lastname: Hu
authors.affiliation: Diabetes research Center of Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Xiu-Yan
authors.initials: XY
authors.lastname: Yang
authors.affiliation: Diabetes research Center of Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Si-Hua
authors.initials: SH
authors.lastname: Gao
authors.affiliation: Diabetes research Center of Beijing University of Chinese Medicine, Beijing, China; Department of Biochemistry and Molecular Biology, Hebei United University, Tangshan, China.
authors.forename: Guang-Jian
authors.initials: GJ
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D005260:Female; D008540:Meiosis; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D010053:Ovary / Q000166:cytology*; D049590:Postpartum Period; D011309:Preservation, Biological; D008941:Spindle Apparatus / Q000378:metabolism; D013696:Temperature; D013997:Time Factors