title: Regulation of the unbalanced redox state in a Schizosaccharomyces pombe tert-butyl hydroperoxide-resistant mutant.
abstract: The one-gene mutation in the tert-butyl hydroperoxide-resistant mutant hyd1-190 of the fission yeast Schizosaccharomyces pombe led to a 4-fold increase in resistance to t-BuOOH and decreased specific concentrations of superoxide and total thiols in comparison with the parental strain hyd+. It suggested an unbalanced redox state of the cells, which induced continuously increased specific activities of glutathione peroxidase, glutathione reductase and glutathione S-transferase and decreased activities of the antioxidant enzymes superoxide dismutases and glucose-6-phosphate dehydrogenase to regulate the redox balance of the mutation-induced permanent, low-level but tolerable internal stress. These results may contribute to the understanding of internal, oxidative stress-related human diseases.
authors.affiliation: University of Pécs Department of General and Environmental Microbiology, Faculty of Sciences Pécs Hungary.
authors.forename: Z
authors.initials: Z
authors.lastname: Gazdag
authors.affiliation: University of Pécs Department of General and Environmental Microbiology, Faculty of Sciences Pécs Hungary.
authors.forename: Nikoletta
authors.initials: N
authors.lastname: Kálmán
authors.affiliation: University of Pécs Institute of Bioanalysis, Faculty of Medicine Pécs Hungary.
authors.forename: Agnes
authors.initials: A
authors.lastname: Blaskó
authors.affiliation: University of Pécs Department of General and Environmental Microbiology, Faculty of Sciences Pécs Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Virág
authors.affiliation: University of Pécs Institute of Biophysics, Faculty of Medicine Pécs Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Belágyi
authors.affiliation: University of Pécs Department of General and Environmental Microbiology, Faculty of Sciences Pécs Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Pesti
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D004351:Drug Resistance / Q000235:genetics; D005838:Genotype; D009154:Mutation; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D010641:Phenotype; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D029702:Schizosaccharomyces pombe Proteins; D013438:Sulfhydryl Compounds; D013481:Superoxides; D020122:tert-Butylhydroperoxide
keywords: oxidative stress; resistance; tert-butyl hydroperoxide; yeast