title: Non-toxic and efficient DNA extractions for soybean leaf and seed chips for high-throughput and large-scale genotyping.
abstract: In applied soybean (Glycine max L.) breeding programs, marker-assisted selection has become a necessity to select value-added quantitative trait loci. The goal of this work was to improve marker-assisted selection workflow by developing a reliable, inexpensive, high-throughput DNA extraction protocol for soybean seed and leaf samples that does not generate hazardous waste. The DNA extraction protocol developed allows for the leverage of robust SNP genotyping platforms such as the Simple Probe Assay and KASPar v4.0 SNP Genotyping System to genotype thousands of seeds or leaves non-destructively in a single day with a 95 % success rate. This methodology makes it possible to run up to 150 SNP markers on the DNA extracted from a single seed chip or leaf sample.
authors.affiliation: Institute for Plant Breeding, Genetics & Genomics, The University of Georgia, Athens, GA, 30602, USA, zrking@uga.edu.
authors.forename: Zachary
authors.initials: Z
authors.lastname: King
authors.forename: Jonathan
authors.initials: J
authors.lastname: Serrano
authors.forename: H
authors.initials: H
authors.lastname: Roger Boerma
authors.forename: Zenglu
authors.initials: Z
authors.lastname: Li
mesh_terms: D018744:DNA, Plant / Q000302:isolation & purification*; D060005:Genotyping Techniques / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods; D018515:Plant Leaves / Q000235:genetics*; D012639:Seeds / Q000235:genetics*; D013025:Glycine max / Q000235:genetics*
chemical_list: D018744:DNA, Plant