title: Integrated strategy of pH-shift and glucose feeding for enhanced production of bioactive Antrodin C in submerged fermentation of Antrodia camphorata.
abstract: Antrodin C is one of the most potent bioactive components produced by the medicinal mushroom Antrodia camphorata. However, almost all studies in this field have focused on the biological activity of Antrodin C and relatively rare information has been reported regarding the biosynthetic process of Antrodin C. In this study, the strategies of pH-shift and glucose feeding for enhanced production of Antrodin C in submerged fermentation of A. camphorata were successfully applied in stirred bioreactors. The critical parameters for pH-shift and glucose feeding were systematically investigated. On one hand, the optimal culture pH for cell growth was distinct with Antrodin C biosynthesis and the maximum Antrodin C production was obtained by maintaining the first-stage culture at initial pH 4.5 and adjusted to 6.0 at day 8. On the other hand, it was beneficial for the Antrodin C accumulation with the initial glucose concentration of 40 g/L and feeding glucose to keep the residual sugar above 10 g/L. The maximum Antrodin C production (1,549.06 mg/L) was about 2.1-fold higher than that of control in 15-L stirred bioreactors by taking advantage of the integrated strategy of pH-shift and glucose feeding. These results would be helpful for the design of a highly efficient Antrodin C biosynthesis process.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.forename: Yong-Dan
authors.initials: YD
authors.lastname: Hu
authors.forename: Rui-Qiu
authors.initials: RQ
authors.lastname: Lu
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Xia
authors.forename: Bo-Bo
authors.initials: BB
authors.lastname: Zhang
authors.forename: Gan-Rong
authors.initials: GR
authors.lastname: Xu
mesh_terms: D055491:Antrodia / Q000378:metabolism / Q000502:physiology*; D019149:Bioreactors; D053898:Biosynthetic Pathways / Q000502:physiology*; D005285:Fermentation; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods; D008301:Maleimides / Q000378:metabolism*
chemical_list: D008301:Maleimides; C552101:antrodin C; D005947:Glucose