title: Glucocorticoids induce apoptosis by inhibiting microRNA cluster miR‑17‑92 expression in chondrocytic cells.
abstract: Sustained treatment with glucocorticoids (GCs) has frequently been observed to impair skeletal development. However, the influence of GCs on chondrocytes, which have a key role in skeletal development, has been rarely reported. HCS‑2/8 cells were selected as an in vitro model of human chondrocytes to assess the apoptosis induced by GCs and determine the role of the microRNA‑17‑92 (miR‑17‑92) cluster in the regulation of apoptosis. It was demonstrated that dexamethasone (Dex) was able to induce apoptosis and high levels of expression of apoptosis‑associated molecules in HCS‑2/8 chondrocytic cells, and that expression of the miR‑17‑92 cluster was inhibited during Dex‑induced apoptosis. In conclusion, the present study suggested that inhibition of the expression of the miR‑17‑92 cluster contributed to the Dex‑induced apoptosis in chondrocytes. The results suggest that microRNAs have an important role in glucocorticoid‑induced impairment to chondrocytes.
authors.affiliation: Department of Spinal Surgery, Second Affiliated Hospital of Inner Mongolia Medical University, Huimin, Hohhot, Inner Mongolia Autonomous Region 010058, P.R. China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Xing
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of Inner Mongolia Medical University, Huimin, Hohhot, Inner Mongolia Autonomous Region 010059, P.R. China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Hao
authors.affiliation: Department of Spinal Surgery, Second Affiliated Hospital of Inner Mongolia Medical University, Huimin, Hohhot, Inner Mongolia Autonomous Region 010058, P.R. China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Spinal Surgery, Second Affiliated Hospital of Inner Mongolia Medical University, Huimin, Hohhot, Inner Mongolia Autonomous Region 010058, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Spinal Surgery, Second Affiliated Hospital of Inner Mongolia Medical University, Huimin, Hohhot, Inner Mongolia Autonomous Region 010058, P.R. China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Huo
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D003907:Dexamethasone / Q000633:toxicity*; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015735:Mifepristone / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D062085:RNA, Long Noncoding; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C568997:MIR17HG, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D062085:RNA, Long Noncoding; D051028:bcl-2-Associated X Protein; D015735:Mifepristone; D003907:Dexamethasone; D053148:Caspase 3