title: Effect of microRNA-145 on IL-1β-induced cartilage degradation in human chondrocytes.
abstract: MicroRNA-145 has been shown to regulate chondrocyte homeostasis. It seems that miR-145 is implicated in cartilage dysfunction in Osteoarthritis (OA). However, the functional role of miR-145 in interleukin-1 beta (IL-1β)-induced extracellular matrix (ECM) degradation of OA cartilage has never been clarified. Here, we show that miR-145 expression increased in OA chondrocytes and in response to IL-1β stimulation. We confirm that mothers against decapentaplegic homolog 3 (Smad3), a key factor in maintaining chondrocyte homeostasis, is directly regulated by miR-145. Modulation of miR-145 affects the expression of Smad3 causing a change of its downstream target gene expression as well as IL-1β-induced ECM degradation in OA chondrocytes. This indicates that miR-145 contributes to impaired ECM in OA cartilage probably in part via targeting Smad3.
authors.affiliation: Laboratory of Biomechanics, Department of Anatomy, Third Military Medical University, Chongqing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, Chengdu Military General Hospital, Chengdu, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Kang
authors.affiliation: Laboratory of Biomechanics, Department of Anatomy, Third Military Medical University, Chongqing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.
authors.forename: Ce
authors.initials: C
authors.lastname: Dou
authors.affiliation: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Kang
authors.affiliation: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.
authors.forename: Jianmei
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Chengdu Military General Hospital, Chengdu, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Quan
authors.affiliation: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. Electronic address: shiwudong@gmail.com.
authors.forename: Shiwu
authors.initials: S
authors.lastname: Dong
mesh_terms: D000328:Adult; D000368:Aged; D053674:Aggrecans / Q000378:metabolism; D001483:Base Sequence; D002356:Cartilage / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D024043:Collagen Type II / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000502:physiology*; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000378:metabolism; D034622:RNA Interference; D051900:Smad3 Protein / Q000378:metabolism; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D053674:Aggrecans; D024043:Collagen Type II; D053583:Interleukin-1beta; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; C497790:SMAD3 protein, human; D051900:Smad3 Protein
keywords: Extracellular matrix; Interleukin-1 beta; MicroRNA; MicroRNA-145; Osteoarthritis