title: Traceback identification of plant components in commercial compound feed through an oligonucleotide microarray based on tubulin intron polymorphism.
abstract: According to EU Regulations, all components of commercial compound feed need to be declared on the label. Effective protection against fraud requires severe controls based on accurate analytical methods to ascertain what is declared by the producers. The aim of this work was to develop an oligonucleotide microarray for the molecular recognition of multiple plant components in commercial feeds. We tested the potential of the highly polymorphic first intron sequences from members of the plant β-tubulin gene family as a target for plant DNA identification. 23 oligonucleotide capture probes, targeting species-specific intron sequences, were assembled within a low density microarray for the identification of 10 plant species, selected from among those most commonly used in cattle feed formulation. The ability of the array to detect specific components in complex flour blends and in compound feed was evaluated.
authors.affiliation: Istituto Biologia e Biotecnologia Agraria, CNR, Via Bassini 5, 20133 Milano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Ponzoni
authors.affiliation: Istituto Biologia e Biotecnologia Agraria, CNR, Via Bassini 5, 20133 Milano, Italy. Electronic address: laura.morello@ibba.cnr.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Morello
authors.affiliation: Istituto Biologia e Biotecnologia Agraria, CNR, Via Bassini 5, 20133 Milano, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Gianì
authors.affiliation: Istituto Biologia e Biotecnologia Agraria, CNR, Via Bassini 5, 20133 Milano, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: Breviario
mesh_terms: D000818:Animals; D002417:Cattle; D017343:Genes, Plant / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D046228:Microarray Analysis; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011110:Polymorphism, Genetic; D014404:Tubulin / Q000235:genetics*
chemical_list: D014404:Tubulin
keywords: Authenticity; Feed; Intron; Microarray; Traceability