title: In vitro antioxidant activity and phenolic content of Cedrus brevifolia bark.
abstract: A raw extract and four extractive fractions were obtained from Cedrus brevifolia (Cyprus cedar) bark. They were all studied regarding the phenolic content and profile using spectrophotometry and HPLC-DAD-ESI-MS. The antioxidant activity was investigated using in vitro assays: DPPH and ABTS radicals scavenging and reducing power assays. The ethyl acetate fraction had the highest total phenolic and proanthocyanidin contents; a taxifolin-O-hexoside, catechin, epicatechin and procyanidin oligomers (three dimers, two trimers) were identified in this fraction. The ethyl acetate fraction was found to possess the highest DPPH and ABTS radicals scavenging effects (EC50 = 13.9 +/- 0.3 and 2.3 +/- 0.0 microg/mL, respectively) and reducing capacity (EC50 = 9.1 +/- 0.1 microg/mL). Antioxidant effects were highly correlated with total phenolic and proanthocyanidin contents (r = 0.89-0.99). These results suggest that Cedrus brevifolia bark is a new source of antioxidants.
authors.forename: Elena
authors.initials: E
authors.lastname: Cretu
authors.forename: Juha-Pekka
authors.initials: JP
authors.lastname: Salminen
authors.forename: Maarit
authors.initials: M
authors.lastname: Karonen
authors.forename: Anca
authors.initials: A
authors.lastname: Miron
authors.forename: Christiana
authors.initials: C
authors.lastname: Charalambous
authors.forename: Andreas I
authors.initials: AI
authors.lastname: Constantinou
authors.forename: Ana Clara
authors.initials: AC
authors.lastname: Aprotosoaie
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D028203:Cedrus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016166:Free Radical Scavengers; D010636:Phenols / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D044945:Proanthocyanidins / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D044945:Proanthocyanidins; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl