title: Conventional techniques to monitor mitochondrial oxygen consumption.
abstract: Following several key discoveries on hypoxia-inducible factors, we have observed an explosion of studies investigating how the hypoxic microenvironment provokes bioenergetic alterations. This is particularly relevant for cancer cells, as they are often exposed to hypoxic conditions in the course of tumor progression. Thus, interest in the measurement of oxygen consumption at the tissue, cell, or mitochondrion level has been revived. Here, we describe the basic principles of cellular respiration and survey some of the conventional methods for measuring O2 consumption in intact or permeabilized cells.
authors.affiliation: Centre de Recherche en Cancérologie de Lyon UMR INSERM U1052/CNRS 5286 Centre Léon Bérard, Lyon, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Simonnet
authors.affiliation: Centre de Recherche en Cancérologie de Lyon UMR INSERM U1052/CNRS 5286 Centre Léon Bérard, Lyon, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Vigneron
authors.affiliation: Institute of Research on Cancer and Aging (IRCAN) CNRS-Inserm-UNS UMR 7284, U 1081 Centre A. Lacassagne, Nice, France; Centre Scientifique de Monaco (CSM), Monaco. Electronic address: pouysseg@unice.fr.
authors.forename: Jacques
authors.initials: J
authors.lastname: Pouysségur
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D019069:Cell Respiration; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism*; D010539:Permeability
chemical_list: D000255:Adenosine Triphosphate; D010100:Oxygen
keywords: ATP; Cancer cell metabolism; HIF-1; Hypoxia; Mitochondrial respiration; Respiratory chain complexes