title: Benzo[a]pyrene modulates the biotransformation, DNA damage and cortisol level of red sea bream challenged with lipopolysaccharide.
abstract: In animals, biotransformation and the immune system interact with each other, however, knowledge of the toxic mechanism of benzo[a]pyrene (BaP) on these two systems is not well known. The present study investigated the toxic effects of BaP on the biotransformation system, cortisol level and DNA integrity of red sea bream (Pagrus major). The results showed that cortisol level was induced under the challenge of lipopolysaccharide (LPS). Short-term exposure (96 h) of BaP at environmental concentration significantly increased the cortisol level, hepatic EROD activity and CYP1A1 mRNA expression. When P. major was exposed to BaP for 14 d followed by LPS challenge this increased the cortisol level, EROD activity and hepatic DNA damage except CYP1A1 mRNA expression. Combined with our previous data, which showed that BaP exposure can modulate the immunologic response in P. major challenged with LPS, a hypothetical adverse outcome pathway of BaP on fish was suggested.
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian 361005, China; State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Bo
authors.affiliation: State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Singaram
authors.initials: S
authors.lastname: Gopalakrishnan
authors.affiliation: State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Fang-Yi
authors.initials: FY
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian 361005, China. Electronic address: wkjian@xmu.edu.cn.
authors.forename: Ke-Jian
authors.initials: KJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D001711:Biotransformation; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004249:DNA Damage; D005399:Fishes / Q000378:metabolism; D006854:Hydrocortisone / Q000378:metabolism*; D008070:Lipopolysaccharides; D008099:Liver / Q000378:metabolism; D021541:Sea Bream / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D001564:Benzo(a)pyrene; D019363:Cytochrome P-450 CYP1A1; D006854:Hydrocortisone
keywords: Adverse outcome pathway; Benzo[a]pyrene; Cytochrome P4501A1; DNA damage; EROD activity