title: Hypercholesterolemic diet induces hepatic steatosis and alterations in mRNA expression of NADPH oxidase in rat livers.
abstract: OBJECTIVE
  This study aimed to determine whether a hypercholesterolemic diet induces hepatic steatosis, alterations in mRNA expression of NADPH oxidase subunits, and antioxidant defenses.
  
  
  METHODS
  Fischer rats were divided into two groups of eight animals according to the treatment, control (C) and hypercholesterolemic diet (H). Those in group C were fed a standard diet (AIN-93M), and those of the group H were fed a hypercholesterolemic diet (25% soybean oil and 1% cholesterol).
  
  
  RESULTS
  The hypercholesterolemic diet did not affect body weight, but resulted in the accumulation of lipids in the liver, increased serum activities of aminotransferases and cholesterol levels. Biomarker of lipid peroxidation (TBARS) and mRNA expression of NADPH oxidase subunits p22(phox) and p47(phox) were increased in the liver of animals in group H. Besides, the activity and expression of antioxidant enzymes were altered.
  
  
  CONCLUSIONS
  The results show increased mRNA expression of NADPH oxidase subunits and changes in antioxidant enzyme activities in diet-induced hepatic steatosis.
authors.affiliation: School of Nutrition, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: Isabel Cristina Mallosto Emerich de
authors.initials: IC
authors.lastname: Abreu
authors.affiliation: Research Center in Biological Sciences, UFOP, Ouro Preto, MG, Brazil.
authors.forename: Joyce Ferreira da Costa
authors.initials: JF
authors.lastname: Guerra
authors.affiliation: Research Center in Biological Sciences, UFOP, Ouro Preto, MG, Brazil.
authors.forename: Renata Rebeca
authors.initials: RR
authors.lastname: Pereira
authors.affiliation: Research Center in Biological Sciences, UFOP, Ouro Preto, MG, Brazil.
authors.forename: Maísa
authors.initials: M
authors.lastname: Silva
authors.affiliation: Research Center in Biological Sciences, UFOP, Ouro Preto, MG, Brazil.
authors.forename: Wanderson Geraldo de
authors.initials: WG
authors.lastname: Lima
authors.affiliation: School of Nutrition, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: Marcelo Eustáquio
authors.initials: ME
authors.lastname: Silva
authors.affiliation: School of Nutrition, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: Pedrosa
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight; D002374:Catalase / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000009:adverse effects*; D059305:Diet, High-Fat / Q000009:adverse effects; D005234:Fatty Liver / Q000209:etiology*; D005260:Female; D005978:Glutathione / Q000032:analysis; D006937:Hypercholesterolemia / Q000209:etiology*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000201:enzymology*; D019255:NADPH Oxidases / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism*; D011916:Rats, Inbred F344; D060888:Real-Time Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
chemical_list: D000975:Antioxidants; D002791:Cholesterol, Dietary; D008055:Lipids; D012333:RNA, Messenger; D014280:Triglycerides; D002784:Cholesterol; D002374:Catalase; D013482:Superoxide Dismutase; D019255:NADPH Oxidases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione