title: pT305-CaMKII stabilizes a learning-induced increase in AMPA receptors for ongoing memory consolidation after classical conditioning.
abstract: The role of CaMKII in learning-induced activation and trafficking of AMPA receptors (AMPARs) is well established. However, the link between the phosphorylation state of CaMKII and the agonist-triggered proteasomal degradation of AMPARs during memory consolidation remains unknown. Here we describe a novel CaMKII-dependent mechanism by which a learning-induced increase in AMPAR levels is stabilized for consolidation of associative long-term memory. Six hours after classical conditioning the levels of both autophosphorylated pT305-CaMKII and GluA1 type AMPAR subunits are significantly elevated in the ganglia containing the learning circuits of the snail Lymnaea stagnalis. CaMKIINtide treatment significantly reduces the learning-induced elevation of both pT305-CaMKII and GluA1 levels and impairs associative long-term memory. Inhibition of proteasomal activity offsets the deleterious effects of CaMKIINtide on both GluA1 levels and long-term memory. These findings suggest that increased levels of pT305-CaMKII play a role in AMPAR-dependent memory consolidation by reducing proteasomal degradation of GluA1 receptor subunits.
authors.affiliation: Sussex Neuroscience, School of Life Sciences, University of Sussex, Brighton, BN1 9QG, UK.
authors.forename: Souvik
authors.initials: S
authors.lastname: Naskar
authors.affiliation: Sussex Neuroscience, School of Life Sciences, University of Sussex, Brighton, BN1 9QG, UK.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wan
authors.affiliation: Sussex Neuroscience, School of Life Sciences, University of Sussex, Brighton, BN1 9QG, UK.
authors.forename: György
authors.initials: G
authors.lastname: Kemenes
mesh_terms: D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism*; D003214:Conditioning, Classical / Q000502:physiology*; D007858:Learning / Q000502:physiology; D008195:Lymnaea; D008568:Memory / Q000502:physiology; D057567:Memory, Long-Term / Q000502:physiology*; D010766:Phosphorylation; D018091:Receptors, AMPA / Q000378:metabolism*
chemical_list: D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D018091:Receptors, AMPA