title: A vesicular stomatitis virus-based mucosal vaccine promotes dendritic cell maturation and elicits preferable immune response against coxsackievirus B3 induced viral myocarditis.
abstract: Recombinant vesicular stomatitis virus (VSV) is widely used as a vaccine platform. However, the capacity of VSV-based vaccines to induce mucosal immunity has not been fully investigated. In the present study, a recombinant VSV expressing coxsackievirus B3 (CVB3) major immunogen VP1 has been generated and the immune protection elicited by VSV-VP1 was evaluated. We demonstrated that intranasal delivery of VSV-VP1 can induce a potent antigen-specific mucosal immune response as well as a systemic immune response, particularly the induction of polyfunctional T cells. Importantly, mice immunized with VSV-VP1 were better protected against CVB3-induced viral myocarditis than those receiving a chitosan-formulated DNA vaccine. Increased dendritic cell (DC) maturation in the mesenteric lymph node (MLN) was observed in the mice vaccinated with VSV-VP1, which could be a potential mechanism for the protective immune response. These findings support VSV as a viral delivery vector that can induce robust mucosal immunity that should be considered for further vaccine development.
authors.affiliation: Soochow University, Jiangsu Key Laboratory of Infection and Immunity Institutes of Biology and Medical Science, Suzhou 215123, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: Soochow University, Jiangsu Key Laboratory of Infection and Immunity Institutes of Biology and Medical Science, Suzhou 215123, China.
authors.forename: Xingjuan
authors.initials: X
authors.lastname: Fan
authors.affiliation: Soochow University, Jiangsu Key Laboratory of Infection and Immunity Institutes of Biology and Medical Science, Suzhou 215123, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yue
authors.affiliation: Soochow University, Jiangsu Key Laboratory of Infection and Immunity Institutes of Biology and Medical Science, Suzhou 215123, China. Electronic address: sdxiongfd@126.com.
authors.forename: Sidong
authors.initials: S
authors.lastname: Xiong
authors.affiliation: Soochow University, Jiangsu Key Laboratory of Infection and Immunity Institutes of Biology and Medical Science, Suzhou 215123, China. Electronic address: chunshengdong@suda.edu.cn.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Dong
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology; D003384:Coxsackievirus Infections / Q000517:prevention & control*; D003713:Dendritic Cells / Q000276:immunology*; D029822:Enterovirus B, Human; D007111:Immunity, Cellular; D018928:Immunity, Mucosal; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000517:prevention & control* / Q000821:virology*; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D018116:Vesiculovirus; D014760:Viral Fusion Proteins; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins; D014765:Viral Vaccines
keywords: Coxsackievirus B3; Dendritic cell; Mucosal vaccine; Polyfunctional T cells; Vesicular stomatitis virus; Viral myocarditis