title: Evaluation of efficacy of combinations of five endodontic sealers with five antibiotics against Enterococcus Faecalis - An in-vitro study.
abstract: BACKGROUND
  To evaluate and compare in vitro the antibacterial efficacy of five antibiotics when added individually to five endodontic sealers against Enterococcus faecalis (EF).
  
  
  METHODS
  This controlled trial with systematic allocation method was carried out to detect the combined antibacterial activity of five endodontic sealers (Kerr sealer EWT, Endomethasone, AH26, AH Plus, Roekoseal) with five antibiotics regularly used (Amoxicillin, metronidazole, azithromyacin, gatifloxacin, doxycycline) on EF. For each sealerantibiotic combinations, thirty BHI agar plates (15 aerobic and 15 anaerobic) were inoculated with EF, containing five sterile paper discs- three of various sealer- antibiotic combinations, one of sealer alone (positive control) & plain disc as negative control were incubated at 370C for 48 hrs and the zone of inhibition was measured. Data analysis was done by ANOVA and Tukey's post- hoc test using SPSS( version 17).
  
  
  RESULTS
  The findings of this study revealed that sealer-antibiotic combination containing amoxicillin had the significant difference (p<0.001) in the mean zone of inhibition compared to other combinations. Metronidazole showed the minimum zone of inhibition among used antibiotics. The sealers in the decreasing order according to their effectiveness on EF were Kerr sealer endomethasone, AH26, Rockseal, AH plus.
  
  
  CONCLUSIONS
  Addition of antibiotics to endodontic sealers enhances their antibacterial activity against Enterococcus faecalis. How to cite the article: Sharma D, Grover R, Pinnameneni PS, Dey S, Raju PR. Evaluation of efficacy of combinations of five endodontic sealers with five antibiotics against Enterococcus Faecalis - An in-vitro study. J Int Oral Health 2014;6(2):90-5.
authors.affiliation: Department of Conservative Dentistry & Endodontics, College of Dental Sciences & Hospital, Indore, Madhya Pradesh, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Sharma
authors.affiliation: Department of Conservative Dentistry & Endodontics, Sardar Patel Dental College, Lucknow, Uttar Pradesh, India.
authors.forename: Rohit
authors.initials: R
authors.lastname: Grover
authors.affiliation: Department of Conservative Dentistry & Endodontics, Saraswarthy Dhanawantri Dental College & Hospital, Parbhani, Maharashtra, India.
authors.forename: Prasanth Sai
authors.initials: PS
authors.lastname: Pinnameneni
authors.affiliation: Department of Conservative Dentistry & Endodontics, Saraswarthy Dhanawantri Dental College & Hospital, Parbhani, Maharashtra, India.
authors.forename: Subhra
authors.initials: S
authors.lastname: Dey
authors.affiliation: Department of Conservative Dentistry & Endodontics, Saraswarthy Dhanawantri Dental College & Hospital, Parbhani, Maharashtra, India.
authors.forename: P Ramakrishnam
authors.initials: PR
authors.lastname: Raju
keywords: Antibiotics; endodontic sealer; endodontic sealer- antibiotic; enterococcous faecalis