title: Endometrial receptivity profile in patients with premature progesterone elevation on the day of HCG administration.
abstract: The impact of a premature elevation of serum progesterone level, the day of hCG administration in patients under controlled ovarian stimulation during IVF procedure, on human endometrial receptivity is still debated. In the present study, we investigated the endometrial gene expression profile shifts during the prereceptive and receptive secretory stage in patients with normal and elevated serum progesterone level on the day of hCG administration in fifteen patients under stimulated cycles. Then, specific biomarkers of endometrial receptivity in these two groups of patients were tested. Endometrial biopsies were performed on oocyte retrieval day and on day 3 of embryo transfer, respectively, for each patient. Samples were analysed using DNA microarrays and qRT-PCR. The endometrial gene expression shift from the prereceptive to the receptive stage was altered in patients with high serum progesterone level (>1.5 ng/mL) on hCG day, suggesting accelerated endometrial maturation during the periovulation period. This was confirmed by the functional annotation of the differentially expressed genes as it showed downregulation of cell cycle-related genes. Conversely, the profile of endometrial receptivity was comparable in both groups. Premature progesterone rise alters the endometrial gene expression shift between the prereceptive and the receptive stage but does not affect endometrial receptivity.
authors.affiliation: CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, 34295 Montpellier, France ; INSERM U1040, Hôpital Saint-Eloi, 34295 Montpellier, France ; Université Montpellier 1, UFR de Médecine, Equipe "Développement Embryonnaire Précoce et Cellules Souches Embryonnaires Humaines", 34000 Montpellier, France.
authors.forename: Delphine
authors.identifier: 0000-0002-4740-8404
authors.initials: D
authors.lastname: Haouzi
authors.affiliation: CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, 34295 Montpellier, France ; INSERM U1040, Hôpital Saint-Eloi, 34295 Montpellier, France ; Université Montpellier 1, UFR de Médecine, Equipe "Développement Embryonnaire Précoce et Cellules Souches Embryonnaires Humaines", 34000 Montpellier, France ; OVO Fertility, 8000 Boulevard Decarie No. 100, Montréal, QC, Canada H4P 2S4.
authors.forename: Laurence
authors.identifier: 0000-0001-7420-2818
authors.initials: L
authors.lastname: Bissonnette
authors.affiliation: CHU Montpellier, ART/PGD Division, Département de Biologie de la Reproduction, Hôpital Arnaud de Villeneuve, 34295 Montpellier, France.
authors.forename: Anna
authors.initials: A
authors.lastname: Gala
authors.affiliation: CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, 34295 Montpellier, France ; INSERM U1040, Hôpital Saint-Eloi, 34295 Montpellier, France ; Université Montpellier 1, UFR de Médecine, Equipe "Développement Embryonnaire Précoce et Cellules Souches Embryonnaires Humaines", 34000 Montpellier, France.
authors.forename: Said
authors.initials: S
authors.lastname: Assou
authors.affiliation: Laboratoire Dynabio, Polyclinique du Cotentin, 50120 Equeurdreville, France.
authors.forename: Frida
authors.identifier: 0000-0001-7891-1048
authors.initials: F
authors.lastname: Entezami
authors.affiliation: CHU Montpellier, Hôpital Gui de Chauliac, Service Anatomie Cytologie Pathologiques, 34295 Montpellier, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Perrochia
authors.affiliation: CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, 34295 Montpellier, France ; INSERM U1040, Hôpital Saint-Eloi, 34295 Montpellier, France ; Université Montpellier 1, UFR de Médecine, Equipe "Développement Embryonnaire Précoce et Cellules Souches Embryonnaires Humaines", 34000 Montpellier, France ; CHU Montpellier, ART/PGD Division, Département de Biologie de la Reproduction, Hôpital Arnaud de Villeneuve, 34295 Montpellier, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Dechaud
authors.affiliation: CHU Léonard de Vinci-Université Paris XIII, Service de Médecine de la Reproduction, Hôpital Jean Verdier, 93143 Bondy, France.
authors.forename: Jean-Noel
authors.initials: JN
authors.lastname: Hugues
authors.affiliation: CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, 34295 Montpellier, France ; INSERM U1040, Hôpital Saint-Eloi, 34295 Montpellier, France ; Université Montpellier 1, UFR de Médecine, Equipe "Développement Embryonnaire Précoce et Cellules Souches Embryonnaires Humaines", 34000 Montpellier, France ; CHU Montpellier, ART/PGD Division, Département de Biologie de la Reproduction, Hôpital Arnaud de Villeneuve, 34295 Montpellier, France.
authors.forename: Samir
authors.initials: S
authors.lastname: Hamamah
mesh_terms: D000328:Adult; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D006063:Chorionic Gonadotropin / Q000008:administration & dosage*; D004624:Embryo Transfer; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006801:Humans; D011374:Progesterone / Q000097:blood*; D012102:Reproductive Control Agents / Q000008:administration & dosage*
chemical_list: D006063:Chorionic Gonadotropin; D012102:Reproductive Control Agents; D011374:Progesterone