title: Surface-modified bacterial nanofibrillar PHB scaffolds for bladder tissue repair.
abstract: The aim of the study is in vitro investigation of the feasibility of surface-modified bacterial nanofibrous poly [(R)-3-hydroxybutyrate] (PHB) graft for bladder reconstruction. In this study, the surface of electrospun bacterial PHB was modified with PEG- or EDA via radio frequency glow discharge method. After plasma modification, contact angle of EDA-modified PHB scaffolds decreased from 110 ± 1.50 to 23 ± 0.5 degree. Interestingly, less calcium oxalate stone deposition was observed on modified PHB scaffolds compared to that of non-modified group. Results of this study show that surface-modified scaffolds not only inhibited calcium oxalate growth but also enhanced the uroepithelial cell viability and proliferation.
authors.affiliation: a Division of Nanotechnology and Nanomedicine, Hacettepe University , Beytepe , Ankara , Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Karahaliloğlu
authors.affiliation: a Division of Nanotechnology and Nanomedicine, Hacettepe University , Beytepe , Ankara , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Demirbilek
authors.affiliation: b Department of Biology , Aksaray University , Aksaray , Turkey.
authors.forename: Mesut
authors.initials: M
authors.lastname: Şam
authors.affiliation: a Division of Nanotechnology and Nanomedicine, Hacettepe University , Beytepe , Ankara , Turkey.
authors.forename: Necdet
authors.initials: N
authors.lastname: Sağlam
authors.affiliation: c UNAM, Institute of Materials Science and Nanotechnology, Bilkent University , Bilkent , Ankara , Turkey.
authors.forename: Alpay Koray
authors.initials: AK
authors.lastname: Mızrak
authors.affiliation: a Division of Nanotechnology and Nanomedicine, Hacettepe University , Beytepe , Ankara , Turkey.|d Division of Biochemistry, Department of Chemistry , Hacettepe University , Beytepe , Ankara , Turkey.
authors.forename: Emir Baki
authors.initials: EB
authors.lastname: Denkbaş
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002129:Calcium Oxalate / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020118:Cupriavidus necator / Q000737:chemistry / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000473:pathology; D005029:Ethylenediamines / Q000737:chemistry; D006885:Hydroxybutyrates / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D007669:Kidney Calculi / Q000737:chemistry; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization; D012038:Regeneration / Q000187:drug effects*; D023822:Tissue Engineering; D001743:Urinary Bladder / Q000187:drug effects* / Q000473:pathology
chemical_list: D001672:Biocompatible Materials; D005029:Ethylenediamines; D006885:Hydroxybutyrates; D002129:Calcium Oxalate; D011092:Polyethylene Glycols; C031234:ethylenediamine
keywords: bladder reconstruction; calcium oxalate stones; nanotexture; poly [(R)-3-hydroxybutyrate] (PHB); tissue engineering