title: Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation.
abstract: Biodegradable electrospun poly(ε-caprolactone) (PCL) scaffolds were coated with platelet-rich plasma (PRP) to improve cell adhesion and proliferation. PRP was obtained from human buffy coat, and tested on human adipose-derived mesenchymal stem cells (MSCs) to confirm cell proliferation and cytocompatibility. Then, PRP was adsorbed on the PCL scaffolds via lyophilization, which resulted in a uniform sponge-like coating of 2.85 (S.D. 0.14) mg/mg. The scaffolds were evaluated regarding mechanical properties (Young's modulus, tensile stress and tensile strain), sustained release of total protein and growth factors (PDGF-BB, TGF-β1 and VEGF), and hemocompatibility. MSC seeded on the PRP-PCL nanofibers showed an increased adhesion and proliferation compared to pristine PCL fibers. Moreover, the adsorbed PRP enabled angiogenesis features observed as neovascularization in a chicken chorioallantoic membrane (CAM) model. Overall, these results suggest that PRP-PCL scaffolds hold promise for tissue regeneration applications.
authors.affiliation: Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, 15872 Santiago de Compostela, Spain; Instituto de Ortopedia y Banco de Tejidos Musculoesqueléticos, Universidad de Santiago de Compostela, 15872 Santiago de Compostela, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Diaz-Gomez
authors.affiliation: Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, 15872 Santiago de Compostela, Spain. Electronic address: carmen.alvarez.lorenzo@usc.es.
authors.forename: Carmen
authors.initials: C
authors.lastname: Alvarez-Lorenzo
authors.affiliation: Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, 15872 Santiago de Compostela, Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Concheiro
authors.affiliation: Instituto de Ortopedia y Banco de Tejidos Musculoesqueléticos, Universidad de Santiago de Compostela, 15872 Santiago de Compostela, Spain.
authors.forename: Maite
authors.initials: M
authors.lastname: Silva
authors.affiliation: Fundación Publica Galega de Medicina Xenómica, Santiago de Compostela, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Dominguez
authors.affiliation: Department of Chemistry, University of Texas Pan American, Edinburg, TX 78541, USA.
authors.forename: Faheem A
authors.initials: FA
authors.lastname: Sheikh
authors.affiliation: Department of Chemistry, University of Texas Pan American, Edinburg, TX 78541, USA.
authors.forename: Travis
authors.initials: T
authors.lastname: Cantu
authors.affiliation: Department of Chemistry, University of Texas Pan American, Edinburg, TX 78541, USA.
authors.forename: Raj
authors.initials: R
authors.lastname: Desai
authors.affiliation: Department of Chemistry, University of Texas Pan American, Edinburg, TX 78541, USA.
authors.forename: Vanessa L
authors.initials: VL
authors.lastname: Garcia
authors.affiliation: Department of Chemistry, University of Texas Pan American, Edinburg, TX 78541, USA. Electronic address: jmacossay@utpa.edu.
authors.forename: Javier
authors.initials: J
authors.lastname: Macossay
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000077214:Becaplermin; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D055119:Elastic Modulus; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D057139:Nanofibers / Q000737:chemistry*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry / Q000633:toxicity; D020574:Proto-Oncogene Proteins c-sis / Q000737:chemistry / Q000378:metabolism; D013718:Tensile Strength; D023822:Tissue Engineering; D053773:Transforming Growth Factor beta1 / Q000737:chemistry / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D020574:Proto-Oncogene Proteins c-sis; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D000077214:Becaplermin; C016240:polycaprolactone
keywords: Angiogenesis; Cell proliferation; Electrospinning; Growth factor sustained release; PCL scaffold; Platelet-rich plasma