title: Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders.
abstract: There are few studies performed for investigating the roles of different ratio and cryoprotectants with dithiothreitol or sucrose on sperm motility characteristics and antioxidant capacities of post-thawed bull spermatozoa. The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations as cryoprotectants and dithiothreitol (D) or sucrose (S) (with/without) as antioxidants in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and diluted using both of the dithiothreitol 5mM or sucrose 25 mM, and control (without additives) was cooled to 4 °C and frozen in 0.25-ml French straws. when compared to control, different doses cryoprotectants and antioxidants addition no significantly increased the percentages of post-thaw sperm progressive and motitilities, acrosome abnormality and plasma membrane integrity (P>0.05). However, EG3+S yielded the greatest percentages of the total abnormality (P<0.05). As regard to antioxidant activities G7 and EG5 led to lowest MDA activity with or without D or S but, these results were not supported to the GPx activity (P<0.01). The sperm motion characteristics such as VAP, VCL, ALH and BCF gave significantly different results (P<0.05). When compared the DNA integrity, different doses cryoprotectants without antioxidants addition significantly increased the percentages of the tail intensity and tail moment (P<0.05). There were no significant differences observed in non-return rates among all treatment groups (P>0.05).
authors.affiliation: Aksaray University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-68100 Aksaray, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Büyükleblebici
authors.affiliation: Aksaray University, Aksaray Vocational School, TR-68100 Aksaray, Turkey. Electronic address: barbarostuncerp@hotmail.com.
authors.forename: Pürhan Barbaros
authors.initials: PB
authors.lastname: Tuncer
authors.affiliation: Selcuk University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-42003 Konya, Turkey.
authors.forename: Mustafa N
authors.initials: MN
authors.lastname: Bucak
authors.affiliation: Aksaray University, Aksaray Vocational School, TR-68100 Aksaray, Turkey.
authors.forename: Umut
authors.initials: U
authors.lastname: Taşdemir
authors.affiliation: Erciyes University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, TR-38039 Kayseri, Turkey.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Eken
authors.affiliation: Aksaray University, Faculty of Veterinary Medicine, Department of Biochemistry, TR-68100 Aksaray, Turkey.
authors.forename: Olga
authors.initials: O
authors.lastname: Büyükleblebici
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Gazi University, TR-06330 Ankara, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Durmaz
authors.affiliation: Erciyes University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-38039 Kayseri, Turkey.
authors.forename: Serpil
authors.initials: S
authors.lastname: Sarıözkan
authors.affiliation: Erciyes University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, TR-38039 Kayseri, Turkey.
authors.forename: Burcu Ü
authors.initials: BÜ
authors.lastname: Endirlik
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D004229:Dithiothreitol / Q000494:pharmacology; D004527:Egg Proteins / Q000494:pharmacology; D004530:Egg Yolk; D019855:Ethylene Glycol / Q000494:pharmacology; D005298:Fertility / Q000187:drug effects; D005307:Fertilization in Vitro / Q000187:drug effects*; D005615:Freezing / Q000009:adverse effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D005990:Glycerol / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D012661:Semen / Q000187:drug effects; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000502:physiology; D013395:Sucrose / Q000494:pharmacology; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D004527:Egg Proteins; D013395:Sucrose; D005979:Glutathione Peroxidase; D019855:Ethylene Glycol; D005990:Glycerol; D004229:Dithiothreitol; D000094765:Glutathione Peroxidase GPX1
keywords: Antioxidants; Bovine; Cryoprotectants; Freeze–thawing; Sperm parameters