title: Investigation of the association of hRRM1 and p53R2 gene polymorphisms in head and neck squamous cell carcinomas.
abstract: Head and neck squamous epithelial cell cancer (HNSCC), the world's fifth most common type of cancers, is associated with short life expectancy and high death rates if not detected in early stages. The aim of this study was to investigate hRRM1 and p53R2 gene polymorphisms by using real-time PCR technique in patients with head and neck cancer. In total, 87 patients with head and neck malignancies and 87 control group who have not any malignancies were included in the study between January 2011 and February 2012 in Istanbul University Faculty of Medicine Department of ORL. In the study, real-time PCR was used to detect hRRM1 (rs12806698 C/A) and p53R2 (rs2290707 G/T) gene polymorphisms in Turkish HNSCC patients and healthy individuals. Genomic DNA isolation was performed according to the kit protocol with spin column. LightCycler 1.5 system was used to perform SNP genotyping using hybridization probes consisting of 3'-fluorescein and a 5'-LightCycler Red labeled pair of oligonucleotide probes. There were significant differences in the distribution of hRRM1 genotypes. Frequency of individuals with hRRM1 AA genotype was higher in patients with less differentiation when compared with well differentiation [p 0.025, Fisher's exact test, odds ratio (OR) 0.140, 95 % confidence intervals (CI) 0.024-0.797]. It is observed that A allele carriers have nearly twofold risk for development of the disease (p = 0.022; χ (2) 5.24; OR 2.02, 95 % CI 1.10-3.72).
authors.affiliation: Department of ORL and Head and Neck Surgery, Istanbul Faculty of Medicine, Istanbul University, 34390, Capa, Fatih, Istanbul, Turkey, ksorhan@istanbul.edu.tr.
authors.forename: Kadir Serkan
authors.initials: KS
authors.lastname: Orhan
authors.forename: Ender
authors.initials: E
authors.lastname: Coskunpinar
authors.forename: Deniz
authors.initials: D
authors.lastname: Kanliada
authors.forename: Yasemin Musteri
authors.initials: YM
authors.lastname: Oltulu
authors.forename: Bora
authors.initials: B
authors.lastname: Basaran
authors.forename: Mehmet
authors.initials: M
authors.lastname: Celik
authors.forename: Bedia
authors.initials: B
authors.lastname: Cakmakoglu
authors.forename: Kemal
authors.initials: K
authors.lastname: Deger
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012262:Ribonucleoside Diphosphate Reductase; D012264:Ribonucleotide Reductases / Q000235:genetics*; D000077195:Squamous Cell Carcinoma of Head and Neck; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D014421:Turkey
chemical_list: D018797:Cell Cycle Proteins; D025521:Tumor Suppressor Proteins; C405947:RRM2B protein, human; D012264:Ribonucleotide Reductases; C475286:RRM1 protein, human; D012262:Ribonucleoside Diphosphate Reductase