title: Association between polymorphisms in the flanking region of the TAFI gene and atherosclerotic cerebral infarction in a Chinese population.
abstract: BACKGROUND
  Atherosclerosis is the leading etiologic factor of Atherosclerotic Cerebral Infarction (ACI). Previous studies have shown that thrombin activatable fibrinolysis inhibitor (TAFI) may play an important role in the occurrence of acute cerebral infarction, and the levels of TAFI are affected by several single nucleotide polymorphisms (SNPs) located in the regulatory and coding regions of the gene encoding TAFI. The present study aimed to determine whether polymorphisms (TAFI -2345 2G/1G, -1690 A/G, -438 A/G, +1583 A/T) of the TAFI gene were associated with ACI in a Han Chinese population.
  
  
  METHODS
  The variant genotypes were identified by restriction fragment length polymorphism (RFLP) and allele-specific polymerase chain reactions (AS-PCR) in 225 patients with ACI and 184 age-matched healthy individuals.
  
  
  RESULTS
  There was a significant difference in the genotype and allele frequencies of TAFI -2345 2G/1G and -1690 A/G polymorphisms between the ACI and control subjects. Further stratification analysis by gender revealed that the presence of the -438 AA genotype and the A allele conferred a higher risk of developing ACI in male patients (p < 0.05). Haplotype analysis demonstrated that four haplotypes of TAFI are significantly associated with ACI.
  
  
  CONCLUSIONS
  Our study provides preliminary evidence that the TAFI -2345 2G/1G and -1690 A/G polymorphisms are associated with ACI susceptibility in a Han Chinese population.
authors.forename: You
authors.initials: Y
authors.lastname: Li
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Zeng
authors.forename: Jianghao
authors.initials: J
authors.lastname: Zhao
authors.forename: Guoda
authors.initials: G
authors.lastname: Ma
authors.forename: Lili
authors.initials: L
authors.lastname: Cui
authors.forename: Hua
authors.initials: H
authors.lastname: Tao
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Lin
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China. yusenchen163@163.com.
authors.forename: Yusen
authors.initials: Y
authors.lastname: Chen
authors.forename: Keshen
authors.initials: K
authors.lastname: Li
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D025901:Carboxypeptidase B2 / Q000235:genetics*; D002544:Cerebral Infarction / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D025901:Carboxypeptidase B2