title: Dendritic polyglycerol-poly(ethylene glycol)-based polymer networks for biosensing application.
abstract: This work describes the formation of a new dendritic polyglycerol-poly(ethylene glycol)-based 3D polymer network as a matrix for immobilization of the redox enzyme periplasmatic aldehyde oxidoreductase to create an electrochemical biosensor. The novel network is built directly on the gold surface, where it simultaneously stabilizes the enzyme for up to 4 days. The prepared biosensors can be used for amperometric detection of benzaldehyde in the range of 0.8-400 μM.
authors.affiliation: Institut für Chemie and Biochemie, Freie Universität Berlin , Takustrasse 3, 14195 Berlin, Germany.
authors.forename: Pradip
authors.initials: P
authors.lastname: Dey
authors.forename: Miriam
authors.initials: M
authors.lastname: Adamovski
authors.forename: Simon
authors.initials: S
authors.lastname: Friebe
authors.forename: Artavazd
authors.initials: A
authors.lastname: Badalyan
authors.forename: Radu-Cristian
authors.initials: RC
authors.lastname: Mutihac
authors.forename: Florian
authors.initials: F
authors.lastname: Paulus
authors.forename: Silke
authors.initials: S
authors.lastname: Leimkühler
authors.forename: Ulla
authors.initials: U
authors.lastname: Wollenberger
authors.forename: Rainer
authors.initials: R
authors.lastname: Haag
mesh_terms: D001547:Benzaldehydes / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D050091:Dendrimers / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
chemical_list: D001547:Benzaldehydes; D050091:Dendrimers; D004800:Enzymes, Immobilized; D011108:Polymers; C043941:polyglycerol; D011092:Polyethylene Glycols; D005949:Glucose Oxidase; D005990:Glycerol; C032175:benzaldehyde