title: Intranasal immunization with live recombinant Lactococcus lactis combined with heat-labile toxin B subunit protects chickens from highly pathogenic avian influenza H5N1 virus.
abstract: Development of safe and effective vaccines to prevent highly pathogenic avian influenza H5N1 virus infection is a challenging goal. Lactococcus lactis (L. lactis) is an ideal delivery vector for vaccine development, and it has been shown previously that oral immunization of encapsulated secretory L. lactis-hemagglutinin (HA) could provide complete protection against homologous H5N1 virus challenge in the mice model. While intranasal immunization is an appealing approach, it is now reported that secretory L. lactis-HA combined with mucosal adjuvant heat-labile toxin B subunit (LTB) could provide protective immunity in the chicken model. As compared to intranasal immunization with L. lactis-HA alone, L. lactis-HA combined with LTB (L. lactis-HA + LTB) could elicit robust neutralizing antibody responses and mucosal IgA responses, as well as strong cellular immune responses in the vaccinated chickens. Importantly, intranasal immunization with L. lactis-HA + LTB could provide 100% protection against H5N1 virus challenge. Taken together, these results suggest that intranasal immunization with L. lactis-HA + LTB can be considered as an effective approach for preventing and controlling infection of H5N1 virus in poultry during an avian influenza A/H5N1 pandemic.
authors.affiliation: Department of Biotechnology, College of Life Science and Food Engineering, Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Han
authors.initials: H
authors.lastname: Lei
authors.forename: Xiaojue
authors.initials: X
authors.lastname: Peng
authors.forename: Handing
authors.initials: H
authors.lastname: Shu
authors.forename: Daxian
authors.initials: D
authors.lastname: Zhao
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D001427:Bacterial Toxins / Q000008:administration & dosage*; D001835:Body Weight; D002645:Chickens; D004337:Drug Carriers / Q000008:administration & dosage*; D004768:Enterotoxins / Q000008:administration & dosage*; D029968:Escherichia coli Proteins / Q000008:administration & dosage*; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000032:analysis; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D013294:Lactococcus lactis / Q000235:genetics*; D016019:Survival Analysis; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D001427:Bacterial Toxins; D004337:Drug Carriers; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007070:Immunoglobulin A; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic; C027538:heat-labile enterotoxin, E coli
keywords: H5N1 virus challenge; L. lactis + LTB; intranasal immunization