title: [Effect of glimepiride on the glucose uptake of rat mandibular osteoblasts in hyperglycemia].
abstract: OBJECTIVE
  To explore the effect of glimepiride on the glucose uptake as well as glucose transporter (GLUT)-1 and GLUT-3 expression levels of rat mandibular osteoblasts in hyperglycemia.
  
  
  METHODS
  Primary osteoblasts were isolated and cultured. Then, the cells were placed in an osteogenic medium containing two glucose concentrations (5.5 and 16.5 mmol X L(-1)), with or without glimepiride (10 micromol x L(-1)). Glucose uptake was determined by employing 18F-deoxyglucose (18F-FDG) in the cells, and GLUT-1 and GLUT-3 expression levels were evaluated by Western blot analysis.
  
  
  RESULTS
  Glucose at 16.5 mmol x L(-1) significantly inhibited 18F-FDG uptake and downregulated GLUT-3 protein expression in osteoblasts. Hyperglycemia increased GLUT-1 protein expression. Glimepiride significantly increased glucose uptake and upregulated GLUT-1 and GLUT-3.
  
  
  CONCLUSIONS
  Glimepiride enhance the glucose transporter in rat osteoblasts at two different glucose concentrations.
authors.forename: Pan
authors.initials: P
authors.lastname: Ma
authors.forename: Baosheng
authors.initials: B
authors.lastname: Tan
authors.forename: Hongchen
authors.initials: H
authors.lastname: Liu
authors.forename: Junli
authors.initials: J
authors.lastname: Ma
authors.forename: Bin
authors.initials: B
authors.lastname: Gu
mesh_terms: D000818:Animals; D019788:Fluorodeoxyglucose F18; D005947:Glucose; D051272:Glucose Transporter Type 1; D006943:Hyperglycemia; D008334:Mandible; D010006:Osteoblasts; D051381:Rats; D013453:Sulfonylurea Compounds
chemical_list: D051272:Glucose Transporter Type 1; C495677:SLC2A1 protein, human; D013453:Sulfonylurea Compounds; D019788:Fluorodeoxyglucose F18; C057619:glimepiride; D005947:Glucose