title: β-lactoglobulin as a vector for β-carotene food fortification.
abstract: Food fortification is a strategy to overcome vitamin A deficiency in developing countries. Our aim was to investigate the involvement of the bovine milk protein β-lactoglobulin (β-Lg), a potential retinoid carrier, in vitamin A absorption. In vivo experiments were conducted by force-feeding mice with retinol or β-carotene associated with either β-Lg or oil-in-water emulsion, with subsequent determination of both vitamin A intestinal mucosa and plasma contents. Caco-2 cells were then used to investigate the mechanisms of vitamin A uptake when delivered by either β-Lg or mixed micelles. We showed that β-Lg was as efficient as emulsion to promote β-carotene, but not retinol, absorption in mice. Similar results were obtained in vitro. Interestingly, an inhibitor of the Scavenger Receptor Class B Type I significantly decreased the uptake of micellar β-carotene but not that of β-carotene bound to β-Lg. Overall, we showed that β-Lg would be a good vector for β-carotene food fortification.
authors.affiliation: Biopolymères Interactions Assemblages, INRA, UR 1268 , F-44316 Nantes, France.
authors.forename: Azza
authors.initials: A
authors.lastname: Mensi
authors.forename: Patrick
authors.initials: P
authors.lastname: Borel
authors.forename: Aurélie
authors.initials: A
authors.lastname: Goncalves
authors.forename: Marion
authors.initials: M
authors.lastname: Nowicki
authors.forename: Béatrice
authors.initials: B
authors.lastname: Gleize
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Roi
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chobert
authors.forename: Thomas
authors.initials: T
authors.lastname: Haertlé
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Reboul
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002417:Cattle; D004337:Drug Carriers / Q000737:chemistry*; D004655:Emulsions / Q000008:administration & dosage / Q000737:chemistry; D005527:Food, Fortified / Q000032:analysis*; D006801:Humans; D007782:Lactoglobulins / Q000737:chemistry*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014801:Vitamin A / Q000008:administration & dosage / Q000737:chemistry; D014802:Vitamin A Deficiency / Q000188:drug therapy*; D019207:beta Carotene / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D004655:Emulsions; D007782:Lactoglobulins; D019207:beta Carotene; D014801:Vitamin A