title: MiR-506 inhibits PRRSV replication in MARC-145 cells via CD151.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important diseases of swine, which is caused by PRRS virus (PRRSV). CD151, one of PRRSV entry mediators, determines the cell susceptibility for PRRSV. Emerging evidence indicates that the host microRNAs (miRNAs) play key roles in modulating virus infection and viral pathogenesis. In the present study, targeting porcine CD151 miRNAs were identified, and their function during PRRSV infection in MARC-145 cells was further verified. We found that miR-506 could directly target porcine CD151 3'-UTR mRNA by luciferase reporter assay. Overexpression of miR-506 significantly decreased CD151 expression at both mRNA and protein levels. Furthermore, overexpression of miR-506 reduced cellular PRRSV replication and virus release in MARC-145 cells. Our results suggested that miR-506 could inhibit PRRSV replication by directly targeting PRRSV receptor of CD151 in MARC-145 cells. However, the molecular mechanisms of miR-506 and its function in vivo need further investigation.
authors.affiliation: Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Provincial Academy of Agricultural Sciences, Wuhan, 430064, People's Republic of China.
authors.forename: Junjing
authors.initials: J
authors.lastname: Wu
authors.forename: Xianwen
authors.initials: X
authors.lastname: Peng
authors.forename: Ao
authors.initials: A
authors.lastname: Zhou
authors.forename: Mu
authors.initials: M
authors.lastname: Qiao
authors.forename: Huayu
authors.initials: H
authors.lastname: Wu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Xiao
authors.forename: Guisheng
authors.initials: G
authors.lastname: Liu
authors.forename: Xinmin
authors.initials: X
authors.lastname: Zheng
authors.forename: Shujun
authors.initials: S
authors.lastname: Zhang
authors.forename: Shuqi
authors.initials: S
authors.lastname: Mei
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D007668:Kidney / Q000276:immunology / Q000378:metabolism / Q000821:virology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D019316:Porcine respiratory and reproductive syndrome virus / Q000254:growth & development* / Q000472:pathogenicity; D012333:RNA, Messenger / Q000378:metabolism; D060185:Tetraspanin 24 / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014779:Virus Replication
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D012333:RNA, Messenger; D060185:Tetraspanin 24