title: Humanized anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles, an antibody conjugate with potent and selective anti-hepatocellular carcinoma activity.
abstract: Low sensitivity of tumor tissue, targeting and sustained release of the drug are bottlenecks of the effect of chemotherapy on hepatocellular carcinoma. In this study, we used the ribosome display technology to screen human anti-VEGFR 2-single-chain antibody (ScFv) that could target directly to VEGFR2, and nanotechnology to prepare As2O3-nanoparticles. Then we built anti-VEGFR-2ScFv-As2O3-stealth nanoparticles using molecular coupling technology, which significantly increased anti-tumor effect while reducing toxicity. The in vivo tissue targeting distribution and anti-tumor effects of the anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles were investigated. Our results showed that anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles could inhibit the development of liver cancer xenograft as a targeting agent and also significantly inhibit angiogenesis.
authors.affiliation: Department of hepatobiliary surgery, 2(nd) affiliated hospital of Nanchang University, n(o) 1 Minde Road, Nanchang, Jiangxi Province 330006, China. Electronic address: ying_xiang_bao@163.com.
authors.forename: Yin
authors.initials: Y
authors.lastname: Xiangbao
authors.affiliation: Department of hepatobiliary surgery, 2(nd) affiliated hospital of Nanchang University, n(o) 1 Minde Road, Nanchang, Jiangxi Province 330006, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Linquan
authors.affiliation: Department of hepatobiliary surgery, 2(nd) affiliated hospital of Nanchang University, n(o) 1 Minde Road, Nanchang, Jiangxi Province 330006, China.
authors.forename: Huang
authors.initials: H
authors.lastname: Mingwen
authors.affiliation: Department of hepatobiliary surgery, 2(nd) affiliated hospital of Nanchang University, n(o) 1 Minde Road, Nanchang, Jiangxi Province 330006, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of hepatobiliary surgery, 2(nd) affiliated hospital of Nanchang University, n(o) 1 Minde Road, Nanchang, Jiangxi Province 330006, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Kai
authors.affiliation: Department of hepatobiliary surgery, 2(nd) affiliated hospital of Nanchang University, n(o) 1 Minde Road, Nanchang, Jiangxi Province 330006, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Department of hepatobiliary surgery, 2(nd) affiliated hospital of Nanchang University, n(o) 1 Minde Road, Nanchang, Jiangxi Province 330006, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Kaiyang
authors.affiliation: Department of hepatobiliary surgery, 2(nd) affiliated hospital of Nanchang University, n(o) 1 Minde Road, Nanchang, Jiangxi Province 330006, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Huaqun
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology / Q000627:therapeutic use*; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000098:blood supply / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D007958:Leukocyte Count; D008113:Liver Neoplasms / Q000097:blood / Q000098:blood supply / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008819:Mice, Nude; D055806:Microvessels / Q000187:drug effects / Q000473:pathology; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000494:pharmacology / Q000627:therapeutic use*; D010976:Platelet Count; D057127:Single-Chain Antibodies / Q000276:immunology*; D014018:Tissue Distribution / Q000187:drug effects; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000276:immunology*
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D057127:Single-Chain Antibodies; D040301:Vascular Endothelial Growth Factor Receptor-2; D000077237:Arsenic Trioxide
keywords: Anti-VEGFR 2-single-chain antibody conjugate; As2O3-stealth nanoparticles; Hepatocellular carcinoma; Ribosome display technology