title: Delivery of bone morphogenetic protein-2 and substance P using graphene oxide for bone regeneration.
abstract: In this study, we demonstrate that graphene oxide (GO) can be used for the delivery of bone morphogenetic protein-2 (BMP-2) and substance P (SP), and that this delivery promotes bone formation on titanium (Ti) implants that are coated with GO. GO coating on Ti substrate enabled a sustained release of BMP-2. BMP-2 delivery using GO-coated Ti exhibited a higher alkaline phosphatase activity in bone-forming cells in vitro compared with bare Ti. SP, which is known to recruit mesenchymal stem cells (MSCs), was co-delivered using Ti or GO-coated Ti to further promote bone formation. SP induced the migration of MSCs in vitro. The dual delivery of BMP-2 and SP using GO-coated Ti showed the greatest new bone formation on Ti implanted in the mouse calvaria compared with other groups. This approach may be useful to improve osteointegration of Ti in dental or orthopedic implants.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Wan-Geun
authors.initials: WG
authors.lastname: La
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Min
authors.initials: M
authors.lastname: Jin
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea ; The National Creative Research Initiative Center for Intelligent Hybrids, Seoul National University, Seoul, Republic of Korea.
authors.forename: Saibom
authors.initials: S
authors.lastname: Park
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hee-Hun
authors.initials: HH
authors.lastname: Yoon
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Gun-Jae
authors.initials: GJ
authors.lastname: Jeong
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Bhang
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea ; The National Creative Research Initiative Center for Intelligent Hybrids, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hoyoung
authors.initials: H
authors.lastname: Park
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea ; The National Creative Research Initiative Center for Intelligent Hybrids, Seoul National University, Seoul, Republic of Korea.
authors.forename: Kookheon
authors.initials: K
authors.lastname: Char
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea ; Institute of Bioengineering, Institute of Chemical Processes, Engineering Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D016503:Drug Delivery Systems / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D051379:Mice; D019736:Prostheses and Implants; D012886:Skull / Q000737:chemistry / Q000166:cytology / Q000187:drug effects / Q000293:injuries; D013373:Substance P / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology
chemical_list: D055396:Bone Morphogenetic Protein 2; D013373:Substance P; D006108:Graphite
keywords: bone morphogenetic protein-2; bone regeneration; graphene oxides; stem cell recruitment; substance P