title: Immunological enhancement action of endotoxin-free tilapia heat shock protein 70 against Streptococcus iniae.
abstract: The immunological effects of heat shock proteins (HSPs) had been found in humans and mice, but scarce data of endotoxin-free Hsp70 were reported in tilapia. In the current study, we reported that tHsp70 alone and antigen-tHsp70 compound increased the proliferations of lymphocytes and macrophages, significantly increased the NO release and phagocytotic ability of macrophages (p<0.05), and enhanced the levels of immune-related genes in lymphocytes and macrophages in a dose- and/or time-dependent manner. On the other hand, tHsp70 not only helped to reduce the proliferation inhibitions induced by the ECP treatment, but also assisted antigens to enhance the vaccine-induced protection against Streptococcus iniae (p<0.05). We described, for the first time, a critical role of endotoxin-free tHsp70 on activation of tilapia lymphocytes and macrophages post S. iniae exposure and its up-regulation effects on vaccine-induced protection. Our research highlights the immunological enhancement action of Hsp70 in teleost immunity.
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China; Institute of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530005, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Li
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: Wanwen
authors.initials: W
authors.lastname: Liang
authors.affiliation: Institute of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530005, China.
authors.forename: Qiuhua
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Huang
authors.affiliation: Institute of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530005, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530005, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Gan
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: Aiying
authors.initials: A
authors.lastname: Lei
authors.affiliation: Institute of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530005, China. Electronic address: wyhuang2005y@yahoo.com.cn.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Huang
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China. Electronic address: newcareer20131415926@gmail.com.
authors.forename: Honglin
authors.initials: H
authors.lastname: Luo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049109:Cell Proliferation; D002478:Cells, Cultured; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000096:biosynthesis; D010587:Phagocytosis / Q000276:immunology; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000276:immunology / Q000662:veterinary*; D022541:Streptococcal Vaccines / Q000276:immunology*; D013291:Streptococcus / Q000276:immunology*; D017210:Tilapia / Q000276:immunology / Q000382:microbiology*; D014611:Vaccination
chemical_list: D029941:Fish Proteins; D018840:HSP70 Heat-Shock Proteins; D022541:Streptococcal Vaccines; D009569:Nitric Oxide
keywords: Heat shock protein 70; Lymphocytes; Macrophages; Streptococcus iniae; Tilapia