title: Association between risk of oral precancer and genetic variations in microRNA and related processing genes.
abstract: BACKGROUND
  MicroRNAs have been implicated in cancer but studies on their role in precancer, such as leukoplakia, are limited. Sequence variations at eight miRNA and four miRNA processing genes were studied in 452 healthy controls and 299 leukoplakia patients to estimate risk of disease.
  
  
  RESULTS
  Genotyping by TaqMan assay followed by statistical analyses showed that variant genotypes at Gemin3 and mir-34b reduced risk of disease [OR = 0.5(0.3-0.9) and OR = 0.7(0.5-0.9) respectively] in overall patients as well as in smokers [OR = 0.58(0.3-1) and OR = 0.68(0.5-0.9) respectively]. Among chewers, only mir29a significantly increased risk of disease [OR = 1.8(1-3)]. Gene-environment interactions using MDR-pt program revealed that mir29a, mir34b, mir423 and Xpo5 modulated risk of disease (p < 0.002) which may be related to change in expression of these genes as observed by Real-Time PCR assays. But association between polymorphisms and gene expressions was not found in our sample set as well as in larger datasets from open access platforms like Genevar and 1000 Genome database.
  
  
  CONCLUSIONS
  Variations in microRNAs and their processing genes modulated risk of precancer but further in-depth study is needed to understand mechanism of disease process.
authors.forename: Roshni
authors.initials: R
authors.lastname: Roy
authors.forename: Navonil
authors.initials: N
authors.lastname: De Sarkar
authors.forename: Sandip
authors.initials: S
authors.lastname: Ghose
authors.forename: Ranjan R
authors.initials: RR
authors.lastname: Paul
authors.forename: Anindita
authors.initials: A
authors.lastname: Ray
authors.forename: Indranil
authors.initials: I
authors.lastname: Mukhopadhyay
authors.affiliation: Human Genetics Unit, Indian Statistical Institute, 203, B,T, Road, Kolkata 700108, India. broy@isical.ac.in.
authors.forename: Bidyut
authors.initials: B
authors.lastname: Roy
mesh_terms: D000328:Adult; D005260:Female; D059647:Gene-Environment Interaction; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007971:Leukoplakia / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012307:Risk Factors
chemical_list: C532045:MIRN29a microRNA, human; C522425:MIRN34 microRNA, human; C548900:MIRN423 microRNA, human; D035683:MicroRNAs