title: Overexpression of the putative extracytoplasmic function sigma (σ) factor FujE enhances FK506 production in Streptomyces sp. strain KCCM 11116P.
abstract: The role of the putative extracytoplasmic function sigma (σ) factor FujE, which has not been characterized as a member of the FK506 biosynthetic gene cluster, on FK506 production was identified by gene deletion, overexpression, and transcription analysis experiments in Streptomyces sp. strain KCCM 11116P. Inactivation of fujE had no effect on FK506 production, growth, or morphological differentiation. Overexpression of fujE with integrative vectors increased FK506 production by 2.87-fold (24.5 ± 1.4 mg·L(-1)) compared with the wild type (8.5 ± 0.5 mg·L(-1)). Semiquantitative reverse transcription-polymerase chain reaction analysis indicated that the overexpression of fujE stimulates the transcription of the FK506 biosynthetic genes. These results demonstrated that fujE is a new member of the FK506 biosynthetic gene cluster.
authors.affiliation: a Division of Bioscience and Bioinformatics, Myongji University, Youngin, Gyeonggi-Do, Korea.
authors.forename: Sung-Kwon
authors.initials: SK
authors.lastname: Lee
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Yang
authors.forename: Choong-Min
authors.initials: CM
authors.lastname: Kang
authors.forename: SangJoon
authors.initials: S
authors.lastname: Mo
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Suh
mesh_terms: D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D007166:Immunosuppressive Agents / Q000378:metabolism*; D005810:Multigene Family; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D016559:Tacrolimus / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D007166:Immunosuppressive Agents; D012808:Sigma Factor; D016559:Tacrolimus
keywords: ECF sigma factor; FK506; FK506 biosynthetic gene; facteur sigma FEC; gene overexpression; gène de la biosynthèse du FK506; surexpression génique