title: Alterations of gene expression and protein synthesis in co-cultured adipose tissue-derived stem cells and squamous cell-carcinoma cells: consequences for clinical applications.
abstract: BACKGROUND
  This is the first study evaluating the interactions of human adipose tissue derived stem cells (ADSCs) and human squamous cell carcinoma cells (SCCs), with regard to a prospective cell-based skin regenerative therapy and a thereby unintended co-localization of ADSCs and SCCs.
  
  
  METHODS
  ADSCs were co-cultured with A431-SCCs and primary SCCs (pSCCs) in a transwell system, and cell-cell interactions were analyzed by assessing doubling time, migration and invasion, angiogenesis, quantitative real time PCR of 229 tumor associated genes, and multiplex protein assays of 20 chemokines and growth factors and eight matrix metalloproteinases (MMPS). Results of co-culture were compared to those of the respective mono-culture.
  
  
  RESULTS
  ADSCs' proliferation on the plate was significantly increased when co-cultured with A431-SCCs (P = 0.038). PSCCs and ADSCs significantly decreased their proliferation in co-culture if cultured on the plate (P <0.001 and P = 0.03). The migration of pSCC was significantly increased in co-culture (P = 0.009), as well as that of ADSCs in A431-SCC-co-culture (P = 0.012). The invasive behavior of pSCCs and A431-SCCs was significantly increased in co-culture by a mean of 33% and 35%, respectively (P = 0.038 and P <0.001). Furthermore, conditioned media from co-cultured ADSC-A431-SCCs and co-cultured ADSCs-pSCCs induced tube formation in an angiogenesis assay in vitro.
  
  
  CONCLUSIONS
  This is the first study evaluating the possible interactions of primary human ADSCs with human SCCs, pointing towards a doubtlessly increased oncological risk, which should not be neglected when considering a clinical use of isolated human ADSCs in skin regenerative therapies.
authors.forename: Eva
authors.initials: E
authors.lastname: Koellensperger
authors.forename: Felix
authors.initials: F
authors.lastname: Gramley
authors.forename: Fabian
authors.initials: F
authors.lastname: Preisner
authors.forename: Uwe
authors.initials: U
authors.lastname: Leimer
authors.forename: Guenter
authors.initials: G
authors.lastname: Germann
authors.forename: Verena
authors.initials: V
authors.lastname: Dexheimer
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018920:Coculture Techniques / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration / Q000502:physiology; D044968:Regenerative Medicine / Q000379:methods; D012867:Skin; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D059467:Transcriptome; D055815:Young Adult