title: Measurement of hydroxyproline in collagen with three different methods.
abstract: Determination of the 4-hydroxy-l-proline (hydroxyproline) concentration may provide useful information for the diagnosis and prognosis of diseases caused by disorders of collagen metabolism. The objective of the present study was to apply liquid chromatography-mass spectrometry (LC-MS) to assess the hydroxyproline concentration. The hydroxyproline concentration in lung and liver tissues measured by LC-MS was compared with values obtained by a colorimetric method, as well as a fluorescence method using high-performance liquid chromatography (HPLC) from previous studies by our group. The determination of the hydroxyproline concentration by LC-MS was improved as compared with that using the colorimetric and HPLC methods, due to its simplicity, high sensitivity (pg level) and short separation time. These results suggested that utilizing the LC-MS method for measuring the hydroxyproline concentration would be advantageous for the diagnosis of diseases associated with abnormalities of collagen metabolism.
authors.affiliation: Department of Gastroenterology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Qiu
authors.affiliation: Genetics Laboratory Shenzhen Longgang District Maternity and Child Healthcare Hospital, Shenzhen, Guangdong 518124, P.R. China.
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Wei
authors.affiliation: Department of Gastroenterology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China.
authors.forename: Xiuzhi
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China.
authors.forename: Binhong
authors.initials: B
authors.lastname: Duan
authors.affiliation: Department of Gastroenterology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Gastroenterology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China.
authors.forename: Jiye
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Qiu
authors.affiliation: Department of Gastroenterology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China.
authors.forename: Wenling
authors.initials: W
authors.lastname: Mu
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000378:metabolism*; D003124:Colorimetry; D004128:Dimethylnitrosamine / Q000633:toxicity; D006909:Hydroxyproline / Q000032:analysis*; D008099:Liver / Q000378:metabolism; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000378:metabolism; D013058:Mass Spectrometry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001761:Bleomycin; D003094:Collagen; D004128:Dimethylnitrosamine; D006909:Hydroxyproline