title: Increased phosphatidylcholine (16:0/16:0) in the folliculus lymphaticus of Warthin tumor.
abstract: Warthin tumor (War-T), the second most common benign salivary gland tumor, consists mainly of neoplastic epithelium and lymphoid stroma. Some proteins and genes thought to be involved in War-T were evaluated by molecular biology and immunology. However, lipids as an important component of many tumor cells have not been well studied in War-T. To elucidate the molecular biology and pathogenesis of War-T, we investigated the visualized distribution of phosphatidylcholines (PCs) by imaging mass spectrometry (IMS). In our IMS analysis of a typical case, 10 signals were significantly different in intensity (p < 0.01) between the War-T and non-tumor (Non-T) regions. Five specific PCs were frequently found in the War-T regions of all of the samples: [PC (16:0/16:0) + K](+) (m/z 772.5), [PC (16:0/20:4) + K](+) (m/z 820.5), [PC (16:0/20:3) + K](+) (m/z 822.5), [PC (18:2/20:4) + K](+) (m/z 844.5), and [PC (18:0/20:5) + K](+) (m/z 846.5). PC (16:0/16:0) was increased specifically in the folliculus lymphaticus of War-T lymphoid stroma, suggesting a different metabolism. Localization of PC (16:0/16:0) might reflect inflammation activity participating in the pathogenesis of War-T. Thus, our IMS analysis revealed the profile of PCs specific to the War-T region. The molecules identified in our study provide important information for further studies of War-T pathogenesis.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Rd, Nanning, 530021, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: He
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Takizawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hayasaka
authors.forename: Noritaka
authors.initials: N
authors.lastname: Masaki
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kusama
authors.forename: Jiping
authors.initials: J
authors.lastname: Su
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mineta
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
mesh_terms: D000235:Adenolymphoma / Q000737:chemistry / Q000378:metabolism*; D008949:Adenoma, Pleomorphic / Q000737:chemistry / Q000378:metabolism*; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010713:Phosphatidylcholines