title: Viability of locus coeruleus cultures in the fourth ventricle of the adult rat.
abstract: Both tyrosine hydroxylase and dopamine-beta-hydroxylase immunocytochemistry indicated the survival of catecholamine neurons and processes in 7-day-old organotype cultures of the locus coeruleus. Cultures of this age were transplanted in the 4th ventricle of adult rats via the cisterna magna. Two months after transplantation microscopic observations revealed that the transplant developed with a defined neural organization. Following tyrosine hydroxylase immunocytochemistry, immunoreactive cells were identified within the transplants. These results indicate that organotype cultures of the locus coeruleus survive within the 4th ventricle of adult rats.
authors.affiliation: Laboratoire L. Lafon, 1 Rue G. Médéric, 94700 Maisons-AlfortsFrance.
authors.forename: M F
authors.initials: MF
authors.lastname: Multon
authors.affiliation: Laboratoire de Médecine Experimentale, Institut de Biologie, Inserm 249, Boulevard Henri IV, 34000 Montpellier, France.
authors.forename: A
authors.initials: A
authors.lastname: McRae-Degueurce