title: [Simultaneous determination of 12 trace elements in children plasma sample by high resolution inductively coupled plasma mass spectrometry].
abstract: OBJECTIVE
  To establish a method of determination of 12 trace elements (B, V, Cr, Mn, Fe, Co, Cu, Zn, Se, Rb, Sr, Mo) in children plasma for the fast,direct dilution by HR-ICP-MS.
  
  
  METHODS
  The plasma samples were reconstituted, centrifuged for 15 minutes, then were added the internal elements of Ga and Y, diluted with 0.5% HNO3 by 1 : 20 (V/V). HR-ICP-MS was optimized and applied for the analysis.
  
  
  RESULTS
  Detection limits were 0.001 - 0.035 microg/L, method detection limits of trace elements were 0.012 - 0.702 microg/L, coefficient of determination (R2) ranged from 0.9983 to 1. 0000 and the 3 levels of spiked recovery were between 85.86% and 121.34%, intra-day and inter-day precision were 0.52% - 5.62% and 1.21% - 10.21%, as well as bovine serum certified samples further verified the accuracy. 12 trace elements in plasma of the children aged 3 -12 years from poor rural in 2002 CNNHS, had been determined by Optimized HR-ICP-MS.
  
  
  CONCLUSIONS
  Preparation of plasma samples with 0.5% HNO3 combined with HR-ICP-MS were applied for a large scale of sample analysis.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Liu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Piao
authors.forename: Weidong
authors.initials: W
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tian
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D002417:Cattle; D002648:Child; D006801:Humans; D007201:Indicator Dilution Techniques; D057230:Limit of Detection; D013058:Mass Spectrometry; D014131:Trace Elements / Q000097:blood*
chemical_list: D014131:Trace Elements