title: Gold nanoparticle aggregation-based colorimetric assay for β-casein detection in bovine milk samples.
abstract: Traditional Kjeldahl method, used for quality evaluation of bovine milk, has intrinsic defects of time-consuming sample preparation and two analyses to determine the difference between non-protein nitrogen content and total protein nitrogen content. Herein, based upon antibody functionalized gold nanoparticles (AuNPs), we described a colorimetric method for β-casein (β-CN) detection in bovine milk samples. The linear dynamic range and the LOD were 0.08-250 μg mL(-1), and 0.03 μg mL(-1) respectively. In addition, the real content of β-CN in bovine milk was measured by using the developed assay. The results are closely correlated with those from Kjeldahl method. The advantages of β-CN triggered AuNP aggregation-based colorimetric assay are simple signal generation, the high sensitivity and specificity as well as no need of complicated sample preparation, which make it for on-site detection of β-CN in bovine milk samples.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China. Electronic address: zhouyu69@sina.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Meng
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: F
authors.initials: F
authors.lastname: Song
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Lu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: H L
authors.initials: HL
authors.lastname: Ren
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: P
authors.initials: P
authors.lastname: Hu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Z S
authors.initials: ZS
authors.lastname: Liu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: J H
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001681:Biological Assay; D002364:Caseins / Q000032:analysis*; D002417:Cattle; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000032:analysis / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D002364:Caseins; D006046:Gold
keywords: Detection; Gold nanoparticle; Milk; β-Casein