title: PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays.
abstract: In this study, magnetic poly(glycidyl methacrylate) microparticles containing carboxyl groups (PGMA-COOH) were coated using highly hydrophilic polymer poly(ethylene glycol) (PEG). PEG was used to reduce nonspecific interactions with proteins and cells while decreasing adhesion of particles to the walls of a microfluidic devices from poly(dimethylsiloxane) (PDMS) and cyclic olefin copolymer (COC). Zeta potential measurement, infrared spectroscopy, scanning electron microscopy, anti-PEG ELISA assay, and bioaffinity interactions between biotin and streptavidin-HRP successfully proved the presence of PEG on the surface of microspheres. Both neat and PEGylated microspheres were then incubated with the inert protein bovine serum albumin or cells to evaluate the rate of nonspecific adsorption (NSA). PEG with Mr of 30,000 Da was responsible for 45% reduction in NSA of proteins and 74% for cells compared to neat particles. The microspheres' behavior in PDMS and COC microchannels was then evaluated. Aggregation and adhesion of PEGylated microspheres significantly decreased compared to neat particles. Finally, the model enzyme horseradish peroxidase was immobilized on the microspheres through the heterobifunctional PEG chain. The possibility for subsequent covalent coupling of the ligand of interest was confirmed. Such PEGylated microparticles can be efficiently used in PDMS microchips as a carrier for bioaffinity separation or of enzyme for catalysis.
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Kucerova
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Svobodova
authors.affiliation: Joint Laboratory of Solid State Chemistry of IMC and University of Pardubice, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Knotek
authors.affiliation: Institute of Environmental and Chemical Engineering, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Palarcik
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 16206 Prague 6, Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Vlcek
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 16206 Prague 6, Czech Republic.
authors.forename: Miloslav
authors.initials: M
authors.lastname: Kincl
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 16206 Prague 6, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Horak
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institute Curie, UMR 168, 26 Rue d'Ulm, 75005 Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Autebert
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institute Curie, UMR 168, 26 Rue d'Ulm, 75005 Paris, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Viovy
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic. Electronic address: zuzana.bilkova@upce.cz.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Bilkova
mesh_terms: D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D004800:Enzymes, Immobilized / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D008280:Magnetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D011109:Polymethacrylic Acids; C042535:polyglycidyl methacrylate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D006735:Horseradish Peroxidase
keywords: Magnetic microspheres; Microfluidics; Nonspecific adsorption; PEGylation