title: Effect of cofactor folate on the growth of Corynebacterium glutamicum SYPS-062 and L-serine accumulation.
abstract: The direct fermentative production of L-serine from sugar has attracted increasing attention. Corynebacterium glutamicum SYPS-062 can directly convert sugar to L-serine. In this study, the effects of exogenous and endogenous regulation of cofactor folate on C. glutamicum SYPS-062 growth and L-serine accumulation were investigated. For exogenous regulation, the inhibitor (sulfamethoxazole) or precursor (p-aminobenzoate) of folate biosynthesis was added to the medium, respectively. For endogenous regulation, the gene (pabAB) that encodes the key enzyme of folate biosynthesis was knocked out or overexpressed to obtain the recombinant C. glutamicum SYPS-062 ΔpabAB and SYPS-062(pJC-tac-pabAB), respectively. The results indicated that decreased levels of cofactor folate supported L-serine accumulation, whereas increased levels of cofactor folate aided in cell growth of C. glutamicum SYPS-062. Thus, this study not only elucidated the role of folate in C. glutamicum SYPS-062 growth and L-serine accumulation, but also provided a novel and convenient approach to regulate folate biosynthesis in C. glutamicum.
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, 1800 Lihu Avenue, 214122, Wuxi, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Xu
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Wenfang
authors.initials: W
authors.lastname: Dou
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Xu
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D003067:Coenzymes / Q000096:biosynthesis / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D005492:Folic Acid / Q000096:biosynthesis / Q000378:metabolism*; D017353:Gene Deletion; D012694:Serine / Q000378:metabolism*; D013420:Sulfamethoxazole / Q000494:pharmacology; D000637:Transaminases / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism
chemical_list: D003067:Coenzymes; D004791:Enzyme Inhibitors; D012694:Serine; D005492:Folic Acid; D000637:Transaminases; C066599:aminodeoxychorismate synthase; D013420:Sulfamethoxazole