title: Porphyromonas gingivalis stimulates IL-6 and IL-8 secretion in GMSM-K, HSC-3 and H413 oral epithelial cells.
abstract: Infection of oral epithelial cells with periodontopathogenic bacteria results in the production of pro-inflammatory cytokines involved in the initiation and progression of periodontal disease. The purpose of this study was to examine the release of interleukin (IL)-6 and IL-8 by oral epithelial cells after exposure to Porphyromonas gingivalis. Non-tumor-derived, immortalized human GMSM-K cells, and human oral squamous cell carcinoma, HSC-3 and H413 cells, were co-cultured with live and heat-inactivated P. gingivalis 2561 (ATCC 33277) and W83 (ATCC BAA-308™). IL-6 and IL-8 were quantified in the culture supernatants after 6 and 24 h. The basal levels of both cytokines and the responses to P. gingivalis were strongly dependent on cell type. GMSM-K cells produced less IL-8 than HSC-3 and H413 cells. Live P. gingivalis induced significant IL-6 and IL-8 secretion in GMSM-K and HSC-3 cells, and heat-inactivation of bacteria enhanced greatly IL-6 and IL-8 stimulation in these cells. Uninfected H413 cells produced high levels of IL-6 and IL-8, but were not responsive to live P. gingivalis; heat-inactivated P. gingivalis up-regulated IL-6 and IL-8 secretion in these cells. Since base-line secretion of IL-6 and IL-8, and responses to P. gingivalis depend on the cell type, conclusions on the responses to P. gingivalis should not be based on studies with a single cell type.
authors.affiliation: Department of Biomedical Sciences, University of the Pacific, Arthur A. Dugoni School of Dentistry, 2155 Webster Street, San Francisco, CA 94115, United States.
authors.forename: Michael
authors.initials: M
authors.lastname: Yee
authors.affiliation: Department of Biomedical Sciences, University of the Pacific, Arthur A. Dugoni School of Dentistry, 2155 Webster Street, San Francisco, CA 94115, United States.
authors.forename: Shawn
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, University of the Pacific, Arthur A. Dugoni School of Dentistry, 2155 Webster Street, San Francisco, CA 94115, United States.
authors.forename: Pushpinder
authors.initials: P
authors.lastname: Sethi
authors.affiliation: Department of Biomedical Sciences, University of the Pacific, Arthur A. Dugoni School of Dentistry, 2155 Webster Street, San Francisco, CA 94115, United States.
authors.forename: Nejat
authors.initials: N
authors.lastname: Düzgüneş
authors.affiliation: Department of Biomedical Sciences, University of the Pacific, Arthur A. Dugoni School of Dentistry, 2155 Webster Street, San Francisco, CA 94115, United States. Electronic address: kkonopka@pacific.edu.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Konopka
mesh_terms: D002460:Cell Line; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D016966:Porphyromonas gingivalis / Q000276:immunology*
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8
keywords: Bacteria-cell interactions; Epithelial cells; Inflammatory response; Interleukin-6; Interleukin-8; Porphyromonas gingivalis