title: Effect of glucocorticoids on development of the membrane resting potential of chick embryo brain cells in culture.
abstract: Progressive increase in membrane resting potential (MRP) values in cultured chick embryo brain neurons were recorded from days 2 to 10 of development. Application of corticosterone or prednisone in 24 hr old cultured cells caused a significant increment in MRP recorded at day 2 of culture with respect to control. When ouabain was added to the corticosterone treated cells its effect on the MRP was impaired. An increment in protein synthesis and a reduction in sodium concentration were also observed in corticosterone pretreated cells. These results show that corticoids accelerate the differentiation process in cultured cells particularly the MRP, stimulating perhaps the Na(+)-K(+) ATPase pump.
authors.affiliation: Departamento de Fisiología, C.I.E.A. del I.P.N. Apdo, Postal 14-740, México, D.F. 07000, México.
authors.forename: M C
authors.initials: MC
authors.lastname: Romano
authors.affiliation: Departamentos de Histología y Fisiología, División de Investigatión, Facultad de Medicina, U.K.A.M. Apdo, Postal 70-250, México, D.F. 04510, México.
authors.forename: P N
authors.initials: PN
authors.lastname: Velázquez
authors.affiliation: Departamentos de Histología y Fisiología, División de Investigatión, Facultad de Medicina, U.K.A.M. Apdo, Postal 70-250, México, D.F. 04510, México.
authors.forename: M B
authors.initials: MB
authors.lastname: Nún̄ez
authors.affiliation: Departamentos de Histología y Fisiología, División de Investigatión, Facultad de Medicina, U.K.A.M. Apdo, Postal 70-250, México, D.F. 04510, México.
authors.forename: B
authors.initials: B
authors.lastname: Fuentes-Pardo