title: Short communication: biofilm production characterization of mecA and mecC methicillin-resistant Staphylococcus aureus isolated from bovine milk in Great Britain.
abstract: Staphylococcus aureus is an important cause of contagious intramammary infection in dairy cattle, and the ability to produce biofilm is considered to be an important virulence property in the pathogenesis of mastitis. The aim of this study was to characterize the biofilm formation capacity of methicillin-resistant Staph. aureus (MRSA), encoding mecA or mecC, isolated from bulk tank milk in Great Britain. For this purpose, 20 MRSA isolates were grown on microtiter plates to determine the biofilm production. Moreover, the spa-typing and the presence of the intercellular adhesion genes icaA and icaD were analyzed by PCR. All MRSA isolates tested belonged to 9 spa-types and were PCR-positive for the ica genes; 10 of them (50%) produced biofilm in the microtiter plate assay. This is also the first demonstration of biofilm production by mecC MRSA.
authors.affiliation: Research and Development Department, Hipra Scientific, Avda. La Selva 135, Amer 17170, Catalonia, Spain. Electronic address: antoni.prenafeta@hipra.com.
authors.forename: A
authors.initials: A
authors.lastname: Prenafeta
authors.affiliation: Research and Development Department, Hipra Scientific, Avda. La Selva 135, Amer 17170, Catalonia, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Sitjà
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.
authors.forename: M A
authors.initials: MA
authors.lastname: Holmes
authors.affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, United Kingdom.
authors.forename: G K
authors.initials: GK
authors.lastname: Paterson
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms; D002417:Cattle; D005260:Female; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D005838:Genotype; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification*; D008892:Milk / Q000382:microbiology*; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000662:veterinary; D006113:United Kingdom
chemical_list: D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus
keywords: Staphylococcus aureus; biofilm; methicillin-resistant Staphylococcus aureus