title: Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3.
abstract: Xenorhabdus indica KB-3, a well-known protease producer, was isolated from its entomopathogenic nematode symbiont Steinernema thermophilum. Since medium constituents are critical to the protease production, the chemical components of the selected medium (soya casein digest broth) were optimized by rotatable central composite design (RCCD) using response surface methodology (RSM). The effects of all five chemical components (considered as independent variables), namely tryptone, soya peptone, dextrose, NaCl, and dipotassium phosphate, on protease production (dependent variable) were studied, and it was found that tryptone and dextrose had maximum influence on protease production. The protease production was increased significantly by 66.31% under optimal medium conditions (tryptone-5.71, soya peptone-4.9, dextrose-1.45, NaCl-6.08, and dipotassium phosphate-0.47 in g/L). To best of knowledge, there are no reports on optimization of medium component for protease production by X. indica KB-3 using RSM and their application in fibrinolysis. This study will be useful for industrial processes for production of protease enzyme from X. indica KB-3 for its application in the field of agriculture and medicine.
authors.affiliation: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India ; Department of Biotechnology, National Institute of Technology, Durgapur 713209, India.
authors.forename: Kumar
authors.initials: K
authors.lastname: Pranaw
authors.affiliation: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: Surender
authors.initials: S
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, National Institute of Technology, Durgapur 713209, India.
authors.forename: Debjani
authors.initials: D
authors.lastname: Dutta
authors.affiliation: Department of Biotechnology, National Institute of Technology, Durgapur 713209, India.
authors.forename: Surabhi
authors.initials: S
authors.lastname: Chaudhuri
authors.affiliation: Division of Nematology, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: Sudershan
authors.initials: S
authors.lastname: Ganguly
authors.affiliation: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: Lata
authors.initials: L
authors.lastname: Nain