title: An integrated membrane system for the biocatalytic production of 3'-sialyllactose from dairy by-products.
abstract: An integrated membrane system was investigated for the production of 3'-sialyllactose by an engineered sialidase using casein glycomacropeptide (CGMP) and lactose as substrates. CGMP was purified by ultrafiltration (UF) to remove any small molecules present and then an enzymatic membrane reactor (EMR) was used to separate the product and reuse the enzyme. A PLCC regenerated cellulose membrane was found to be the most suitable for both the UF purification and EMR. Subsequently, nanofiltration (NF) was conducted to increase the purity of the 3'-sialyllactose by removing the excess lactose present. The NTR7450 membrane outperformed others in NF due to its high retention of 3'-sialyllactose (98%) and relatively low rejection of lactose (40%). The lactose in the permeate could be concentrated by the NF45 membrane and recycled into the EMR. The described integrated membrane system enables a more economic and efficient enzymatic production of 3'-sialyllactose.
authors.affiliation: Department of Chemical and Biochemical Engineering, Center for BioProcess Engineering, Building 229, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. Electronic address: jluo@kt.dtu.dk.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Chemical and Biochemical Engineering, Center for BioProcess Engineering, Building 229, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Rune T
authors.initials: RT
authors.lastname: Nordvang
authors.affiliation: Department of Chemical and Biochemical Engineering, Center for BioProcess Engineering, Building 229, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Sofie T
authors.initials: ST
authors.lastname: Morthensen
authors.affiliation: Department of Chemical and Biochemical Engineering, Center for BioProcess Engineering, Building 229, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Birgitte
authors.initials: B
authors.lastname: Zeuner
authors.affiliation: Department of Chemical and Biochemical Engineering, Center for BioProcess Engineering, Building 229, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Anne S
authors.initials: AS
authors.lastname: Meyer
authors.affiliation: Department of Chemical and Biochemical Engineering, Center for BioProcess Engineering, Building 229, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Jørn Dalgaard
authors.initials: JD
authors.lastname: Mikkelsen
authors.affiliation: Department of Chemical and Biochemical Engineering, Center for BioProcess Engineering, Building 229, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. Electronic address: mp@kt.dtu.dk.
authors.forename: Manuel
authors.initials: M
authors.lastname: Pinelo
mesh_terms: D000818:Animals; D002364:Caseins / Q000378:metabolism*; D002384:Catalysis; D002482:Cellulose; D002852:Chromatography, Ion Exchange; D006020:Glycopeptides / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism*; D008567:Membranes, Artificial; D008892:Milk / Q000737:chemistry*; D015394:Molecular Structure; D009439:Neuraminidase / Q000138:chemical synthesis / Q000378:metabolism*; D009844:Oligosaccharides / Q000096:biosynthesis*; D014462:Ultrafiltration
chemical_list: C421467:3'-sialyllactose; D002364:Caseins; D006020:Glycopeptides; D008567:Membranes, Artificial; D009844:Oligosaccharides; C020251:kappa-casein glycomacropeptide; D002482:Cellulose; D009439:Neuraminidase; D007785:Lactose
keywords: EMR; Enzyme; Human milk oligosaccharides; Nanofiltration; Ultrafiltration