title: Predominance of rotavirus G1[P8] genotype among under-five children with gastroenteritis in Mwanza, Tanzania.
abstract: We analyzed stool samples from underfives with gastroenteritis for rotavirus infection between January 2010 and June 2011. A total of 393 stool specimens were examined for rotavirus infection using enzyme-linked immunosorbent assay (ELISA). Hundred selected positive specimens were genotyped using multiplex polymerase chain reaction. Out of 393 underfives, 194 (49.4%) had rotavirus infection, with 96.9% of infected underfives being <2 years. Underfives infected with rotavirus had prolonged hospital stay than those without rotavirus infection (P = 0.0001). G1 was the most predominant G type (59%) followed by G8 (13%) while P[8] was the most predominant P type (25%). In single-type infection, common G-P combinations were G1P[8] (24%) and G1P[6] (17%). Common mixed infections were G1/G8 (16%) and P4/P8 (13%). G1 genotype is common among underfives with gastroenteritis in Mwanza. Diversity of genotypes causing gastroenteritis in Mwanza necessitates a continuous surveillance after the introduction of RotaRix® vaccine.
authors.affiliation: Department of Pediatrics and Child Health Bugando Medical Centre, P.O.Box 1370, Mwanza, Tanzania.
authors.forename: Adolfine
authors.initials: A
authors.lastname: Hokororo
authors.affiliation: Department of Biochemistry and Molecular Biology Catholic University of Health and Allied Sciences, P.O.Box 1464, Mwanza, Tanzania.
authors.forename: Benson R
authors.initials: BR
authors.lastname: Kidenya
authors.affiliation: Department of Microbiology and Immunology Catholic University of Health and Allied Sciences, P.O.Box 1464, Mwanza, Tanzania.
authors.forename: Jeremiah
authors.initials: J
authors.lastname: Seni
authors.affiliation: Department of Virology, University of Limpopo, College of Health and Allied Sciences, P.O.Box 173, Limpopo, South Africa.
authors.forename: Seheri
authors.initials: S
authors.lastname: Mapaseka
authors.affiliation: Department of Virology, University of Limpopo, College of Health and Allied Sciences, P.O.Box 173, Limpopo, South Africa.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Mphahlele
authors.affiliation: Department of Microbiology and Immunology Catholic University of Health and Allied Sciences, P.O.Box 1464, Mwanza, Tanzania mshana72@yahoo.com.
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Mshana
mesh_terms: D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D060885:Multiplex Polymerase Chain Reaction; D011446:Prospective Studies; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000235:genetics* / Q000821:virology*; D013636:Tanzania / Q000453:epidemiology
chemical_list: D012367:RNA, Viral
keywords: Tanzania; genotypes; rotavirus