title: Poly(2-hydroxyethyl methacrylate)-b-poly(L-Lysine) cationic hybrid materials for non-viral gene delivery in NIH 3T3 mouse embryonic fibroblasts.
abstract: In order to develop efficient and nontoxic gene delivery vectors, a series of biocompatible block copolymers, poly[(2-hydroxyethyl methacrylate)40 -block-(L-lysine)n ] (n = 40, 80, 120, 150), are prepared by combining an atom transfer radical polymerization of 2-hydroxyethyl methacrylate with a ring-opening polymerization of N(ϵ) -(carbobenzoxy)-L-lysine N-carboxyanhydride. The block copolymers are successfully condensed with plasmid DNA (pDNA) into nanosized (<200 nm) polyplexes. As a representative sample, p(HEMA)40 -b-p(lys)150 is utilized to confirm the effective cellular and nuclear uptake of pDNA. The polymer/pDNA polyplexes exhibit very low cytotoxicity and enhanced transfection activity by being easily taken up into mouse embryonic fibroblast cell line (NIH 3T3). Thus, the chimeric block copolymers provide a means for developing versatile nonviral gene vectors harboring the ideal requirements of low cytotoxicity, good stability, and high transfection efficiency for gene therapy.
authors.affiliation: Department of Polymer Science and Engineering, BK21 PLUS Centre for Advanced Chemical Technology, Pusan National University, Republic of Korea.
authors.forename: Renjith P
authors.initials: RP
authors.lastname: Johnson
authors.forename: Saji
authors.initials: S
authors.lastname: Uthaman
authors.forename: Johnson V
authors.initials: JV
authors.lastname: John
authors.forename: Min Seon
authors.initials: MS
authors.lastname: Heo
authors.forename: In Kyu
authors.initials: IK
authors.lastname: Park
authors.forename: Hongsuk
authors.initials: H
authors.lastname: Suh
authors.forename: Il
authors.initials: I
authors.lastname: Kim
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000494:pharmacology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry / Q000494:pharmacology; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry / Q000494:pharmacology; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology
chemical_list: D011107:Polylysine; D011102:Polyhydroxyethyl Methacrylate; D004247:DNA
keywords: ATRP; cationic block copolymers; gene delivery; polypeptide; polyplex