title: Analysis of Phaseolus vulgaris response to its association with Trichoderma harzianum (ALL-42) in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani.
abstract: The present study was carried out to evaluate the ability of Trichoderma harzianum (ALL 42-isolated from Brazilian Cerrado soil) to promote common bean growth and to modulate its metabolism and defense response in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani using a proteomic approach. T. harzianum was able to promote common bean plants growth as shown by the increase in root/foliar areas and by size in comparison to plants grown in its absence. The interaction was shown to modulate the expression of defense-related genes (Glu1, pod3 and lox1) in roots of P. vulgaris. Proteomic maps constructed using roots and leaves of plants challenged or unchallenged by T. harzianum and phytopathogenic fungi showed differences. Reference gels presented differences in spot distribution (absence/presence) and relative volumes of common spots (up or down-regulation). Differential spots were identified by peptide fingerprinting MALDI-TOF mass spectrometry. A total of 48 identified spots (19 for leaves and 29 for roots) were grouped into protein functional classes. For leaves, 33%, 22% and 11% of the identified proteins were categorized as pertaining to the groups: metabolism, defense response and oxidative stress response, respectively. For roots, 17.2%, 24.1% and 10.3% of the identified proteins were categorized as pertaining to the groups: metabolism, defense response and oxidative stress response, respectively.
authors.affiliation: Department of Cellular Biology, University of Brasilia (UNB), Brasilia, Distrito Federal, Brazil.
authors.forename: Jackeline L
authors.initials: JL
authors.lastname: Pereira
authors.affiliation: Department of Cellular Biology, University of Brasilia (UNB), Brasilia, Distrito Federal, Brazil.
authors.forename: Rayner M L
authors.initials: RM
authors.lastname: Queiroz
authors.affiliation: Department of Cellular Biology, University of Brasilia (UNB), Brasilia, Distrito Federal, Brazil.
authors.forename: Sébastien O
authors.initials: SO
authors.lastname: Charneau
authors.affiliation: Department of Cellular Biology, University of Brasilia (UNB), Brasilia, Distrito Federal, Brazil.
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Felix
authors.affiliation: Department of Cellular Biology, University of Brasilia (UNB), Brasilia, Distrito Federal, Brazil.
authors.forename: Carlos A O
authors.initials: CA
authors.lastname: Ricart
authors.affiliation: Department of Cellular Biology, University of Brasilia (UNB), Brasilia, Distrito Federal, Brazil.
authors.forename: Francilene Lopes
authors.initials: FL
authors.lastname: da Silva
authors.affiliation: Department of Cellular Biology, University of Brasilia (UNB), Brasilia, Distrito Federal, Brazil.
authors.forename: Andrei Stecca
authors.initials: AS
authors.lastname: Steindorff
authors.affiliation: Biological Sciences Institute, Federal University of Goiás (UFG), Goiânia, Goiás, Brazil.
authors.forename: Cirano J
authors.initials: CJ
authors.lastname: Ulhoa
authors.affiliation: Department of Cellular Biology, University of Brasilia (UNB), Brasilia, Distrito Federal, Brazil.
authors.forename: Eliane F
authors.initials: EF
authors.lastname: Noronha
mesh_terms: D000595:Amino Acid Sequence; D005670:Fusarium / Q000254:growth & development / Q000502:physiology*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D027805:Phaseolus / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D012232:Rhizoctonia / Q000254:growth & development / Q000502:physiology*; D014242:Trichoderma / Q000502:physiology*
chemical_list: D010940:Plant Proteins