title: Melittin modifies bending elasticity in an unexpected way.
abstract: Understanding the molecular mechanism of the interaction of amphipathic and antimicrobial peptides with membranes is of fundamental interest, especially because of the potential of amphipathic peptides as therapeutics. The most studied amphipathic peptides in this context are certainly melittin, magainin and alamethicin, of which melittin is the only one to exhibit a powerful hemolytic and therefore toxic action. Herein we study the effect of the antimicrobial but hemolytic peptide melittin on the bending elasticity of giant unilamellar vesicles (GUVs). The results are compared to the effects of non-hemolytic amphipathic peptides such as alamethicin. We found that monomeric melittin acts very differently on the membrane mechanical properties. Strikingly, the difference is the most pronounced for low peptide concentrations, relevant for the hemolytic action. This gives some insight into the subtle nature of this peptide-membrane interaction. Furthermore, the results show that bending elasticity measurements might be a sensitive way to distinguish between lytic and non-lytic antimicrobial peptides.
authors.affiliation: Ecole Nationale Supérieure de Chimie de Rennes, CNRS UMR 6226, 11 allée de Beaulieu CS 50837, F-35708 Rennes Cedex 7, France; Université Européenne de Bretagne, France.
authors.forename: Tanja
authors.initials: T
authors.lastname: Pott
authors.affiliation: Ecole Nationale Supérieure de Chimie de Rennes, CNRS UMR 6226, 11 allée de Beaulieu CS 50837, F-35708 Rennes Cedex 7, France; Université Européenne de Bretagne, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Gerbeaud
authors.affiliation: Ecole Nationale Supérieure de Chimie de Rennes, CNRS UMR 6226, 11 allée de Beaulieu CS 50837, F-35708 Rennes Cedex 7, France; Université Européenne de Bretagne, France.
authors.forename: Nina
authors.initials: N
authors.lastname: Barbier
authors.affiliation: Ecole Nationale Supérieure de Chimie de Rennes, CNRS UMR 6226, 11 allée de Beaulieu CS 50837, F-35708 Rennes Cedex 7, France; Université Européenne de Bretagne, France. Electronic address: philippe.meleard@ensc-rennes.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Méléard
mesh_terms: D001696:Biomechanical Phenomena; D004548:Elasticity; D006461:Hemolysis; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008555:Melitten / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D053835:Unilamellar Liposomes / Q000737:chemistry / Q000378:metabolism
chemical_list: D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D053835:Unilamellar Liposomes; D008555:Melitten; C027891:1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine
keywords: Bending elasticity; Diacylglycerophosphatidylcholine; Giant vesicles; Lysis; Melittin; X-ray diffraction