title: LncRNAs expression signatures of hepatocellular carcinoma revealed by microarray.
abstract: OBJECTIVE
  To analyze the expression profiles of long non-coding RNAs (lncRNAs) in hepatocellular carcinoma.
  
  
  METHODS
  Hepatocellular carcinoma (HCC) tissues and matched adjacent non-tumor (NT) liver tissues were collected from 29 patients with HCC, immediately after liver resection, between March 2011 and July 2013. The diagnosis of HCC was made based on histological examination. Differentially expressed lncRNAs between HCC and NT tissues were revealed through microarray-based lncRNAs expression profiling. Further, quantification of selected lncRNAs was performed using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  Six hundred and fifty-nine lncRNAs were differentially expressed between HCC and NT tissues, of which five [TCONS_00018278, AK093543, D16366, ENST00000501583, NR_002819 (MALAT1)] were selected for validation. Four of them were significantly downregulated in HCC tissues compared with NT tissues (P = 0.012, 0.045, 0.000 and 0.000, respectively), and the expression level of MALAT1 showed no significant difference (P = 0.114).
  
  
  CONCLUSIONS
  This study identified a set of lncRNAs differentially expressed in HCC tissues and provided useful information for exploring potential therapeutic targets and diagnostic biomarkers of this cancer.
authors.affiliation: Wu-Tao Liu, Xin Lu, Guang-Hua Tang, Jin-Jun Ren, Wen-Jun Liao, Peng-Lei Ge, Jie-Fu Huang, Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Wu-Tao
authors.initials: WT
authors.lastname: Liu
authors.affiliation: Wu-Tao Liu, Xin Lu, Guang-Hua Tang, Jin-Jun Ren, Wen-Jun Liao, Peng-Lei Ge, Jie-Fu Huang, Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.affiliation: Wu-Tao Liu, Xin Lu, Guang-Hua Tang, Jin-Jun Ren, Wen-Jun Liao, Peng-Lei Ge, Jie-Fu Huang, Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Tang
authors.affiliation: Wu-Tao Liu, Xin Lu, Guang-Hua Tang, Jin-Jun Ren, Wen-Jun Liao, Peng-Lei Ge, Jie-Fu Huang, Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Ren
authors.affiliation: Wu-Tao Liu, Xin Lu, Guang-Hua Tang, Jin-Jun Ren, Wen-Jun Liao, Peng-Lei Ge, Jie-Fu Huang, Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Liao
authors.affiliation: Wu-Tao Liu, Xin Lu, Guang-Hua Tang, Jin-Jun Ren, Wen-Jun Liao, Peng-Lei Ge, Jie-Fu Huang, Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Peng-Lei
authors.initials: PL
authors.lastname: Ge
authors.affiliation: Wu-Tao Liu, Xin Lu, Guang-Hua Tang, Jin-Jun Ren, Wen-Jun Liao, Peng-Lei Ge, Jie-Fu Huang, Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Jie-Fu
authors.initials: JF
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011446:Prospective Studies; D062085:RNA, Long Noncoding / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D062085:RNA, Long Noncoding
keywords: Expression signature; Hepatocellular carcinoma; Long non-coding RNA; MALAT1; Microarray