title: Identification of a novel cognate cytosolic Hsp70 gene (MnHsc70-2) from oriental river prawn Macrobrachium nipponense and comparison of its expressions with the first cognate Hsc70 (MnHsc70-1) under different stresses.
abstract: The 70-kDa family of heat-shock proteins (Hsp70) plays an important role in the host immunity, which is widely expressed in eukaryotic cells as a major chaperone protein. In the present study, the full-length complementary DNA (cDNA) of a second cognate cytosolic Hsp70 family member (MnHsc70-2) was cloned and characterized from Macrobrachium nipponense, which is an economically and nutritionally important crustacean. The cDNA was 2,717 bp, containing an open reading frame (ORF) of 1,950 bp, which encodes a protein of 649 amino acids with a theoretical molecular weight of 71.1 kDa and an isoelectric point of 5.27. Sequence alignment showed that the MnHsc70-2 shared 75-97 % identity with other heat-shock proteins. Compared to the previously identified cognate Hsp70 (MnHsc70-1) in M. nipponense, MnHsc70-2 showed quite different expression profiles under unstressed conditions in all tested tissues, including the hemocytes, heart, hepatopancreas, gill, intestine, nerve, and muscle. The phylogenetic analysis demonstrated that MnHsc70-2 showed the closest relationship with MnHsc70-1. Heat-inducibility assays showed that two isolated messenger RNAs (mRNAs) displayed different expression profiles in both the hepatopancreas and gill tissues. MnHsc70-1 mRNA expression level decreased at first and then increased to the normal level, whereas MnHsc70-2 mRNA level increased at first and then decreased. The expressions of two MnHsc70s showed substantial obvious heat-inducible regulation in both the hepatopancreas and gill. Under bacterial challenge by Aeromonas hydrophila, both MnHsc70-1 and MnHsc70-2 mRNA level was up-regulated moderately. The results suggested that two cognate Hsc70s may play essential functions in mediating responses to heat-shock and bacterial challenge.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1# Wenyuan Road, 210023, Nanjing, People's Republic of China.
authors.forename: Yunji
authors.initials: Y
authors.lastname: Xiu
authors.forename: Jia
authors.initials: J
authors.lastname: Feng
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Lu
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.forename: Huanxi
authors.initials: H
authors.lastname: Zhu
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Wenjie
authors.initials: W
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Meng
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
mesh_terms: D016980:Aeromonas hydrophila / Q000472:pathogenicity; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism*; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response; D054884:Host-Pathogen Interactions; D006358:Hot Temperature; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D033541:Palaemonidae / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger