title: Intravital imaging of mouse colonic adenoma using MMP-based molecular probes with multi-channel fluorescence endoscopy.
abstract: Intravital imaging has provided molecular, cellular and anatomical insight into the study of tumor. Early detection and treatment of gastrointestinal (GI) diseases can be enhanced with specific molecular markers and endoscopic imaging modalities. We present a wide-field multi-channel fluorescence endoscope to screen GI tract for colon cancer using multiple molecular probes targeting matrix metalloproteinases (MMP) conjugated with quantum dots (QD) in AOM/DSS mouse model. MMP9 and MMP14 antibody (Ab)-QD conjugates demonstrate specific binding to colonic adenoma. The average target-to-background (T/B) ratios are 2.10 ± 0.28 and 1.78 ± 0.18 for MMP14 Ab-QD and MMP9 Ab-QD, respectively. The overlap between the two molecular probes is 67.7 ± 8.4%. The presence of false negative indicates that even more number of targeting could increase the sensitivity of overall detection given heterogeneous molecular expression in tumors. Our approach indicates potential for the screening of small or flat lesions that are precancerous.
authors.affiliation: School of Mechatronics, Gwangju Institute of Science and Technology, Gwangju, South Korea .
authors.forename: Gyungseok
authors.initials: G
authors.lastname: Oh
authors.affiliation: Department of Medical System Engineering, Gwangju Institute of Science and Technology, Gwangju, South Korea .
authors.forename: Su Woong
authors.initials: SW
authors.lastname: Yoo
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, South Korea .
authors.forename: Yebin
authors.initials: Y
authors.lastname: Jung
authors.affiliation: Asan Institute for Life sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Yeon-Mi
authors.initials: YM
authors.lastname: Ryu
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, South Korea .
authors.forename: Youngrong
authors.initials: Y
authors.lastname: Park
authors.affiliation: Asan Institute for Life sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea ; Deparment of Medicine, University of Ulsan College of Medicine, Seoul, South Korea .
authors.forename: Sang-Yeob
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Division of Integrative Biosciences and Biotechnology and Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, South Korea.
authors.forename: Ki Hean
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, South Korea .
authors.forename: Sungjee
authors.initials: S
authors.lastname: Kim
authors.affiliation: Asan Institute for Life sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea ; Department of Gastroenterology, Asan Medical Center,University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Myung
authors.affiliation: School of Mechatronics, Gwangju Institute of Science and Technology, Gwangju, South Korea , ; Department of Medical System Engineering, Gwangju Institute of Science and Technology, Gwangju, South Korea .
authors.forename: Euiheon
authors.initials: E
authors.lastname: Chung
keywords: (110.0110) Imaging systems; (170.2150) Endoscopic imaging; (170.3880) Medical and biological imaging; (170.4580) Optical diagnostics for medicine