title: Surface modification of polycaprolactone scaffolds fabricated via selective laser sintering for cartilage tissue engineering.
abstract: Surface modified porous polycaprolactone scaffolds fabricated via rapid prototyping techniques were evaluated for cartilage tissue engineering purposes. Polycaprolactone scaffolds manufactured by selective laser sintering (SLS) were surface modified through immersion coating with either gelatin or collagen. Three groups of scaffolds were created and compared for both mechanical and biological properties. Surface modification with collagen or gelatin improved the hydrophilicity, water uptake and mechanical strength of the pristine scaffold. From microscopic observations and biochemical analysis, collagen-modified scaffold was the best for cartilage tissue engineering in terms of cell proliferation and extracellular matrix production. Chondrocytes/collagen-modified scaffold constructs were implanted subdermally in the dorsal spaces of female nude mice. Histological and immunohistochemical staining of the retrieved implants after 8 weeks revealed enhanced cartilage tissue formation. We conclude that collagen surface modification through immersion coating on SLS-manufactured scaffolds is a feasible scaffold for cartilage tissue engineering in craniofacial reconstruction.
authors.affiliation: Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan, ROC; Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Craniofacial Research Center, Chang Gung University, Kweishann, Taoyuan 333, Taiwan, ROC.
authors.forename: Chih-Hao
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Graduate Institute of Medical Mechatronics, Chang Gung University, Kweishan, Taoyuan 333, Taiwan, ROC.
authors.forename: Ming-Yih
authors.initials: MY
authors.lastname: Lee
authors.affiliation: Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Craniofacial Research Center, Chang Gung University, Kweishann, Taoyuan 333, Taiwan, ROC.
authors.forename: Victor Bong-Hang
authors.initials: VB
authors.lastname: Shyu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Craniofacial Research Center, Chang Gung University, Kweishann, Taoyuan 333, Taiwan, ROC.
authors.forename: Yi-Chieh
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Craniofacial Research Center, Chang Gung University, Kweishann, Taoyuan 333, Taiwan, ROC.
authors.forename: Chien-Tzung
authors.initials: CT
authors.lastname: Chen
authors.affiliation: Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan, ROC; Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Kweishan, Taoyuan 333, Taiwan, ROC. Electronic address: jpchen@mail.cgu.edu.tw.
authors.forename: Jyh-Ping
authors.initials: JP
authors.lastname: Chen
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D002356:Cartilage / Q000166:cytology / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D007834:Lasers; D051379:Mice; D008819:Mice, Nude; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D012038:Regeneration / Q000187:drug effects; D013499:Surface Properties; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes; D011091:Polyesters; C016240:polycaprolactone; D003094:Collagen
keywords: Cartilage tissue engineering; Chondrocytes; Collagen; Gelatin; Immersion coating; Selective laser sintering