title: Bone-marrow mesenchymal stem cell transplantation to treat diabetic nephropathy in tree shrews.
abstract: Diabetic nephropathy (DN) is a common microvascular complication of diabetes. We used a new DN model in tree shrews to validate the use of bone-marrow mesenchymal stem cell (BM-MSC) transplantation to treat DN. The DN tree shrew model was established by a high-sugar and high-fat diet and four injections of streptozotocin. 4',6-Diamidino-2-phenylindole labelled BM-MSCs were injected into tree shrews. The DN tree shrew model was successfully established. Blood glucose was significantly increased ( p < 0.01) during the entire experiment. DN tree shrews showed dyslipidemia, insulin resistance and increased 24-h proteinuria. At 21 days after BM-MSC transplantation, glucose and levels of triglycerides, total cholesterol and 24-h urine volume were lower than in tree shrews with DN alone ( p < 0.01) but were still higher than control values ( p < 0.01). Levels of creatinine and urea nitrogen as well as 24-h proteinuria were lower for DN tree shrews with BM-MSCs transplantation than DN alone ( p < 0.05). High-sugar and high-fat diet combined with STZ injection can induce a tree shrew model of DN. BM-MSCs injection can home to damaged kidneys and pancreas, for reduced 24-h proteinuria and improved insulin resistance.
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Pan
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yang
authors.forename: Xiang
authors.initials: X
authors.lastname: Yao
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Sun
authors.forename: Lu
authors.initials: L
authors.lastname: Zhu
authors.forename: Jin-Xiang
authors.initials: JX
authors.lastname: Wang
authors.forename: Rong-Qing
authors.initials: RQ
authors.lastname: Pang
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Cai
authors.forename: Jie-Jie
authors.initials: JJ
authors.lastname: Dai
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Ruan
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001806:Blood Urea Nitrogen; D001854:Bone Marrow Cells / Q000166:cytology*; D002784:Cholesterol / Q000097:blood; D003404:Creatinine / Q000097:blood; D003928:Diabetic Nephropathies / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005919:Glomerular Filtration Rate; D017127:Glycation End Products, Advanced / Q000097:blood; D007328:Insulin / Q000097:blood; D007668:Kidney / Q000473:pathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010179:Pancreas / Q000473:pathology; D013311:Streptozocin / Q000633:toxicity; D014280:Triglycerides / Q000097:blood; D014418:Tupaiidae
chemical_list: D001786:Blood Glucose; D017127:Glycation End Products, Advanced; D007328:Insulin; D014280:Triglycerides; D013311:Streptozocin; D002784:Cholesterol; D003404:Creatinine
keywords: DAPI; bone-marrow mesenchymal stem cells; diabetic nephropathy; transplantation; tree shrews