title: [Hemolysis influence on twenty-two biochemical parameters measurement].
abstract: The study of the influence of hemolysis was determined experimentally for twenty two biochemical parameters on the analyzer Cobas 6000 ce (Roche Diagnostics). The addition method of hemolysate was used to create an increasing concentration of hemoglobin ranging from 0 to 2000 μmol/L. The limit of 10% variation was chosen to define the influence of hemolysis on the measurement. The parameters studied were classified into several categories: the parameters for which hemolysis does not influence the measurement: albumin, uric acid, calcium, C-reactive protein, myoglobin, NT -pro BNP, S100 protein, and urea; parameters impacted positively leading to an overestimation of the result: aspartate aminotransferase, total cholesterol, creatine kinase, creatinine, lactate dehydrogenase, magnesium, magnesium, total protein, triglycerides; and negatively impacted settings so causing an underestimation of the result: alanine amino- transferase, gamma glutamyl transferase, lipase, alkaline phosphatase, troponin T hypersensitive. Certain parameters influence of hemolysis varies depending on the magnitude of the measured parameter this interference being observed for normal values but disappearing for pathological values: creatinine, cholesterol, alkaline phosphatase, triglycerides, or the inverse interference is greater than for conventional pathological values: lipase, alanine amino-transferase. Knowledge of this variability interference allows the biologist to adapt its methods of reporting in the case of haemolysed samples.
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Ali
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Sacchetto
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Erwan
authors.initials: E
authors.lastname: Dumontet
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Le Carrer
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Orsonneau
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Delaroche
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Edith
authors.initials: E
authors.lastname: Bigot-Corbel
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001774:Blood Chemical Analysis / Q000706:statistics & numerical data*; D001798:Blood Proteins / Q000032:analysis; D002097:C-Reactive Protein / Q000032:analysis; D002118:Calcium / Q000097:blood; D002784:Cholesterol / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D003404:Creatinine / Q000097:blood; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis / Q000502:physiology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008049:Lipase / Q000097:blood; D008274:Magnesium / Q000097:blood; D009211:Myoglobin / Q000097:blood; D020097:Natriuretic Peptide, Brain / Q000097:blood; D010446:Peptide Fragments / Q000097:blood; D011498:Protein Precursors / Q000097:blood; D009418:S100 Proteins / Q000097:blood; D012709:Serum Albumin / Q000032:analysis; D014280:Triglycerides / Q000097:blood; D020107:Troponin T / Q000097:blood; D014508:Urea / Q000097:blood; D014527:Uric Acid / Q000097:blood; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D001798:Blood Proteins; D006454:Hemoglobins; D009211:Myoglobin; D010446:Peptide Fragments; D011498:Protein Precursors; D009418:S100 Proteins; D012709:Serum Albumin; D014280:Triglycerides; D020107:Troponin T; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain; D014527:Uric Acid; D014508:Urea; D002097:C-Reactive Protein; D002784:Cholesterol; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase; D008049:Lipase; D000469:Alkaline Phosphatase; D008274:Magnesium; D002118:Calcium
keywords: hemoglobinemia; hemolysis; interference; overestimation; underestimation