title: Histidine methylation of yeast ribosomal protein Rpl3p is required for proper 60S subunit assembly.
abstract: Histidine protein methylation is an unusual posttranslational modification. In the yeast Saccharomyces cerevisiae, the large ribosomal subunit protein Rpl3p is methylated at histidine 243, a residue that contacts the 25S rRNA near the P site. Rpl3p methylation is dependent upon the presence of Hpm1p, a candidate seven-beta-strand methyltransferase. In this study, we elucidated the biological activities of Hpm1p in vitro and in vivo. Amino acid analyses reveal that Hpm1p is responsible for all of the detectable protein histidine methylation in yeast. The modification is found on a polypeptide corresponding to the size of Rpl3p in ribosomes and in a nucleus-containing organelle fraction but was not detected in proteins of the ribosome-free cytosol fraction. In vitro assays demonstrate that Hpm1p has methyltransferase activity on ribosome-associated but not free Rpl3p, suggesting that its activity depends on interactions with ribosomal components. hpm1 null cells are defective in early rRNA processing, resulting in a deficiency of 60S subunits and translation initiation defects that are exacerbated in minimal medium. Cells lacking Hpm1p are resistant to cycloheximide and verrucarin A and have decreased translational fidelity. We propose that Hpm1p plays a role in the orchestration of the early assembly of the large ribosomal subunit and in faithful protein production.
authors.affiliation: Department of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: Qais
authors.initials: Q
authors.lastname: Al-Hadid
authors.affiliation: Department of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Roy
authors.affiliation: Department of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: William
authors.initials: W
authors.lastname: Munroe
authors.affiliation: Department of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Dzialo
authors.affiliation: Department of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: Guillaume F
authors.initials: GF
authors.lastname: Chanfreau
authors.affiliation: Department of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Los Angeles, California, USA clarke@mbi.ucla.edu.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Clarke
mesh_terms: D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D006639:Histidine / Q000235:genetics / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D012322:RNA Precursors; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C065039:RNA, ribosomal, 25S; D006639:Histidine; D008780:Methyltransferases