title: Fate mapping reveals origin and dynamics of lymph node follicular dendritic cells.
abstract: Follicular dendritic cells (FDCs) regulate B cell function and development of high affinity antibody responses but little is known about their biology. FDCs associate in intricate cellular networks within secondary lymphoid organs. In vitro and ex vivo methods, therefore, allow only limited understanding of the genuine immunobiology of FDCs in their native habitat. Herein, we used various multicolor fate mapping systems to investigate the ontogeny and dynamics of lymph node (LN) FDCs in situ. We show that LN FDC networks arise from the clonal expansion and differentiation of marginal reticular cells (MRCs), a population of lymphoid stromal cells lining the LN subcapsular sinus. We further demonstrate that during an immune response, FDCs accumulate in germinal centers and that neither the recruitment of circulating progenitors nor the division of local mature FDCs significantly contributes to this accumulation. Rather, we provide evidence that newly generated FDCs also arise from the proliferation and differentiation of MRCs, thus unraveling a critical function of this poorly defined stromal cell population.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2 Marseille, France Institut National de la Santé et de la Recherche Médicale (INSERM), UMR_S 1104 Marseille, France Centre National de la Recherche Scientifique (CNRS), UMR7280 Marseille, France Aix-Marseille Univ (AMU), F-13284 Marseille, France.
authors.forename: Meryem
authors.initials: M
authors.lastname: Jarjour
authors.affiliation: Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2 Marseille, France Institut National de la Santé et de la Recherche Médicale (INSERM), UMR_S 1104 Marseille, France Centre National de la Recherche Scientifique (CNRS), UMR7280 Marseille, France Aix-Marseille Univ (AMU), F-13284 Marseille, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Jorquera
authors.affiliation: Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2 Marseille, France Institut National de la Santé et de la Recherche Médicale (INSERM), UMR_S 1104 Marseille, France Centre National de la Recherche Scientifique (CNRS), UMR7280 Marseille, France Aix-Marseille Univ (AMU), F-13284 Marseille, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Mondor
authors.affiliation: Institute of Pathology, Charité Universitätsmedizin Berlin, 10117 Berlin, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Wienert
authors.affiliation: Center for Immunology and Infection, Department of Biology and Hull York Medical School, University of York, YO10 5DD York, England, UK.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Narang
authors.affiliation: Center for Immunology and Infection, Department of Biology and Hull York Medical School, University of York, YO10 5DD York, England, UK.
authors.forename: Mark C
authors.initials: MC
authors.lastname: Coles
authors.affiliation: Institute of Pathology, Charité Universitätsmedizin Berlin, 10117 Berlin, Germany.
authors.forename: Frederick
authors.initials: F
authors.lastname: Klauschen
authors.affiliation: Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2 Marseille, France Institut National de la Santé et de la Recherche Médicale (INSERM), UMR_S 1104 Marseille, France Centre National de la Recherche Scientifique (CNRS), UMR7280 Marseille, France Aix-Marseille Univ (AMU), F-13284 Marseille, France bajenoff@ciml.univ-mrs.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Bajénoff
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology*; D049109:Cell Proliferation; D020566:Dendritic Cells, Follicular / Q000276:immunology* / Q000378:metabolism; D018858:Germinal Center / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D053245:RANK Ligand / Q000276:immunology / Q000378:metabolism; D017463:Receptors, Complement 3b / Q000276:immunology / Q000378:metabolism; D017464:Receptors, Complement 3d / Q000276:immunology / Q000378:metabolism; D013234:Stem Cells / Q000276:immunology / Q000378:metabolism; D017154:Stromal Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D008164:Luminescent Proteins; D053245:RANK Ligand; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d; C505689:Tnfsf11 protein, mouse