title: Studies on the effect of quercetin and nitrates on the redox homeostasis using in vitro model.
abstract: Antioxidants are widely considered to be a preventive measure for many diseases and beneficial for health. However, an increasing number of reports suggest a lack of any influence by antioxidants on health or even harmful pro-oxidative effects of antioxidants. In most cases, the research was conducted with respect to a chosen antioxidant, without considering the presence of other chemical substances present in food, with which these compounds may react. The aim of this work was to determine whether and to what extent the simultaneous presence of quercetin and sodium nitrate influences oxidative-reductive homeostasis in fibroblast cultures. Superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), and nitric oxide synthase (NOS) activities were measured together with nitric oxide (NO) concentration and total antioxidant status (TAS). An increase in the activity of all the enzymes measured and in the NO concentration was determined compared with the control culture. The most prominent changes were observed at the highest quercetin concentration. These results indicate that the simultaneous presence of quercetin and sodium nitrate disrupts the oxidative-reductive homeostasis in fibroblasts.
authors.affiliation: Medical University of Silesia, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Department of Food and Nutrition, 8 Jednosci Street, 41-200 Sosnowiec, Poland. Electronic address: ekurzeja@sum.edu.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Kurzeja
authors.affiliation: Medical University of Silesia, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Department of Food and Nutrition, 8 Jednosci Street, 41-200 Sosnowiec, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Stec
authors.affiliation: Medical University of Silesia, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Department of Food and Nutrition, 8 Jednosci Street, 41-200 Sosnowiec, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Synowiec-Wojtarowicz
authors.affiliation: Medical University of Silesia, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Department of Food and Nutrition, 8 Jednosci Street, 41-200 Sosnowiec, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Jowsa
authors.affiliation: Medical University of Silesia, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Department of Food and Nutrition, 8 Jednosci Street, 41-200 Sosnowiec, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pawłowska-Góral
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D008807:Mice, Inbred BALB C; D009566:Nitrates / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010084:Oxidation-Reduction; D011794:Quercetin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D009566:Nitrates; D009569:Nitric Oxide; D000255:Adenosine Triphosphate; C031618:sodium nitrate; D011794:Quercetin; D005979:Glutathione Peroxidase; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase
keywords: Fibroblasts; Quercetin; Redox homeostasis; Sodium nitrate