title: On the origins of the mitotic shift in proliferating cell layers.
abstract: BACKGROUND
  During plant and animal development, monolayer cell sheets display a stereotyped distribution of polygonal cell shapes. In interphase cells these shapes range from quadrilaterals to decagons, with a robust average of six sides per cell. In contrast, the subset of cells in mitosis exhibits a distinct distribution with an average of seven sides. It remains unclear whether this 'mitotic shift' reflects a causal relationship between increased polygonal sidedness and increased division likelihood, or alternatively, a passive effect of local proliferation on cell shape.
  
  
  METHODS
  We use a combination of probabilistic analysis and mathematical modeling to predict the geometry of mitotic polygonal cells in a proliferating cell layer. To test these predictions experimentally, we use Flp-Out stochastic labeling in the Drosophila wing disc to induce single cell clones, and confocal imaging to quantify the polygonal topologies of these clones as a function of cellular age. For a more generic test in an idealized cell layer, we model epithelial sheet proliferation in a finite element framework, which yields a computationally robust, emergent prediction of the mitotic cell shape distribution.
  
  
  RESULTS
  Using both mathematical and experimental approaches, we show that the mitotic shift derives primarily from passive, non-autonomous effects of mitoses in neighboring cells on each cell's geometry over the course of the cell cycle. Computationally, we predict that interphase cells should passively gain sides over time, such that cells at more advanced stages of the cell cycle will tend to have a larger number of neighbors than those at earlier stages. Validating this prediction, experimental analysis of randomly labeled epithelial cells in the Drosophila wing disc demonstrates that labeled cells exhibit an age-dependent increase in polygonal sidedness. Reinforcing these data, finite element simulations of epithelial sheet proliferation demonstrate in a generic framework that passive side-gaining is sufficient to generate a mitotic shift.
  
  
  CONCLUSIONS
  Taken together, our results strongly suggest that the mitotic shift reflects a time-dependent accumulation of shared cellular interfaces over the course of the cell cycle. These results uncover fundamental constraints on the relationship between cell shape and cell division that should be general in adherent, polarized cell layers.
authors.forename: William T
authors.initials: WT
authors.lastname: Gibson
authors.forename: Boris Y
authors.initials: BY
authors.lastname: Rubinstein
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Meyer
authors.forename: James H
authors.initials: JH
authors.lastname: Veldhuis
authors.forename: G Wayne
authors.initials: GW
authors.lastname: Brodland
authors.forename: Radhika
authors.initials: R
authors.lastname: Nagpal
authors.affiliation: Stowers Institute for Medical Research, 64110 Kansas City, MO, USA. mg2@stowers.org.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Gibson
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004330:Drosophila; D008938:Mitosis; D008954:Models, Biological; D011336:Probability