title: Altered glutamyl-aminopeptidase activity and expression in renal neoplasms.
abstract: BACKGROUND
  Advances in the knowledge of renal neoplasms have demonstrated the implication of several proteases in their genesis, growth and dissemination. Glutamyl-aminopeptidase (GAP) (EC. 3.4.11.7) is a zinc metallopeptidase with angiotensinase activity highly expressed in kidney tissues and its expression and activity have been associated wtih tumour development.
  
  
  METHODS
  In this prospective study, GAP spectrofluorometric activity and immunohistochemical expression were analysed in clear-cell (CCRCC), papillary (PRCC) and chromophobe (ChRCC) renal cell carcinomas, and in renal oncocytoma (RO). Data obtained in tumour tissue were compared with those from the surrounding uninvolved kidney tissue. In CCRCC, classic pathological parameters such as grade, stage and tumour size were stratified following GAP data and analyzed for 5-year survival.
  
  
  RESULTS
  GAP activity in both the membrane-bound and soluble fractions was sharply decreased and its immunohistochemical expression showed mild staining in the four histological types of renal tumours. Soluble and membrane-bound GAP activities correlated with tumour grade and size in CCRCCs.
  
  
  CONCLUSIONS
  This study suggests a role for GAP in the neoplastic development of renal tumours and provides additional data for considering the activity and expression of this enzyme of interest in the diagnosis and prognosis of renal neoplasms.
authors.forename: Lorena
authors.initials: L
authors.lastname: Blanco
authors.forename: Begoña
authors.initials: B
authors.lastname: Sanz
authors.forename: Itxaro
authors.initials: I
authors.lastname: Perez
authors.forename: Clara E
authors.initials: CE
authors.lastname: Sánchez
authors.forename: M Luz
authors.initials: ML
authors.lastname: Cándenas
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Pinto
authors.forename: Javier
authors.initials: J
authors.lastname: Gil
authors.forename: Luis
authors.initials: L
authors.lastname: Casis
authors.forename: José I
authors.initials: JI
authors.lastname: López
authors.affiliation: Department of Physiology, Faculty of Medicine and Dentistry, Universitiy of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain. gorka.larrinaga@ehu.es.
authors.forename: Gorka
authors.initials: G
authors.lastname: Larrinaga
mesh_terms: D018249:Adenoma, Oxyphilic / Q000235:genetics / Q000473:pathology; D000368:Aged; D000809:Angiotensins / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002291:Carcinoma, Papillary / Q000235:genetics / Q000473:pathology; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D010450:Endopeptidases / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D043384:Glutamyl Aminopeptidase / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis
chemical_list: D000809:Angiotensins; D014408:Biomarkers, Tumor; C076819:angiotensinase; D010450:Endopeptidases; D043384:Glutamyl Aminopeptidase