title: Thiolated human serum albumin cross-linked dextran hydrogels as a macroscale delivery system.
abstract: Hydrogels play an important role in macroscale delivery systems by enabling the transport of cells and molecules. Here we present a facile and benign method to prepare a dextran-based hydrogel (Dex-sHSA) using human serum albumin (HSA) as a simultaneous drug carrier and covalent cross-linker. Drug binding affinity of the albumin protein was conserved in the thiolation step using 2-iminothiolane and subsequently, in the in situ gelation step. Oscillation rheometry studies confirmed the formation of a three-dimensional viscoelastic network upon reaction of dextran and the HSA protein. The mechanical properties of Dex-sHSA hydrogel can be tuned by the protein concentration, and the degree of thiolation of sHSA. Sustained release of hydrophobic drugs, such as ibuprofen, paclitaxel and dexamethasone, from the Dex-sHSA network was shown over one week. Hence, this albumin-based dextran hydrogel system demonstrates its potential as a macroscale delivery system of hydrophobic therapeutics for a wide range of biomedical applications.
authors.affiliation: Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands. a.kros@chem.leidenuniv.nl.
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
authors.forename: Roxanne E
authors.initials: RE
authors.lastname: Kieltyka
authors.forename: Wim
authors.initials: W
authors.lastname: Jesse
authors.forename: Ben
authors.initials: B
authors.lastname: Norder
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Korobko
authors.forename: Alexander
authors.initials: A
authors.lastname: Kros
mesh_terms: D003907:Dexamethasone / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry*; D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D007052:Ibuprofen / Q000737:chemistry; D017239:Paclitaxel / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D003911:Dextrans; D004337:Drug Carriers; D020100:Hydrogels; D012709:Serum Albumin; D003907:Dexamethasone; D004228:Dithionitrobenzoic Acid; D017239:Paclitaxel; D007052:Ibuprofen