title: Temporal focusing-based multiphoton excitation microscopy via digital micromirror device.
abstract: This Letter presents an enhanced temporal focusing-based multiphoton excitation (MPE) microscope in which the conventional diffraction grating is replaced by a digital micromirror device (DMD). Experimental results from imaging a thin fluorescence film show that the 4.0 μm axial resolution of the microscope is comparable with that of a setup incorporating a 600  lines/mm grating; hence, the optical sectioning ability of the proposed setup is demonstrated. Similar to a grating, the DMD diffracts illuminating light frequencies for temporal focusing; additionally, it generates arbitrary patterns. Since the DMD is placed on the image-conjugate plane of the objective lens' focal plane, the MPE pattern can be projected on the focal plane precisely.
authors.forename: Jenq-Nan
authors.initials: JN
authors.lastname: Yih
authors.forename: Yvonne Yuling
authors.initials: YY
authors.lastname: Hu
authors.forename: Yong Da
authors.initials: YD
authors.lastname: Sie
authors.forename: Li-Chung
authors.initials: LC
authors.lastname: Cheng
authors.forename: Chi-Hsiang
authors.initials: CH
authors.lastname: Lien
authors.forename: Shean-Jen
authors.initials: SJ
authors.lastname: Chen
mesh_terms: D004867:Equipment Design; D007909:Lenses; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D055096:Optical Devices; D055620:Optical Phenomena