title: Syntheses and structural investigation of some alkali metal ion-mediated LV(V)O2(-) (L(2-) = tridentate ONO ligands) species: DNA binding, photo-induced DNA cleavage and cytotoxic activities.
abstract: Eight alkali metal ion-mediated dioxidovanadium(v), [{V(V)O2L(1-6)}A(H2O)n]∝, complexes for A = Li(+), Na(+), K(+) and Cs(+), containing tridentate aroylhydrazonate ligands coordinating via ONO donor atoms, are described. All the synthesised ligands and the metal complexes were successfully characterised by elemental analysis, IR, UV-Vis and NMR spectroscopy. X-ray crystallographic investigation of 3, 5-7 shows the presence of distorted NO4 coordination geometries for LVO2(-) in each case, and varying μ-oxido and/or μ-aqua bridging with interesting variations correlated with the size of the alkali metal ions: with small Li(+), no bridging-O is found but four ion aggregates are found with Na(+), chains for K(+) and finally, layers for Cs(+). Two (5) or three-dimensional (3, 6 and 7) architectures are consolidated by hydrogen bonding. The dioxidovanadium(v) complexes were found to exhibit DNA binding activity due to their interaction with CT-DNA by the groove binding mode, with binding constants ranging from 10(3) to 10(4) M(-1). Complexes 1-8 were also tested for DNA nuclease activity against pUC19 plasmid DNA which showed that 6 and 7 had the best DNA binding and photonuclease activity; these results support their good protein binding and cleavage activity with binding constants ranging from 10(4) to 10(5) M(-1). Finally, the in vitro antiproliferative activity of all complexes was assayed against the HeLa cell line. Some of the complexes (2, 5, 6 and 7) show considerable activity compared to commonly used chemotherapeutic drugs. The variation in cytotoxicity of the complexes is influenced by the various functional groups attached to the aroylhydrazone derivative.
authors.affiliation: Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, India. rupamdinda@nitrkl.ac.in.
authors.forename: Subhashree P
authors.initials: SP
authors.lastname: Dash
authors.forename: Alok K
authors.initials: AK
authors.lastname: Panda
authors.forename: Sagarika
authors.initials: S
authors.lastname: Pasayat
authors.forename: Rupam
authors.initials: R
authors.lastname: Dinda
authors.forename: Ashis
authors.initials: A
authors.lastname: Biswas
authors.forename: Edward R T
authors.initials: ER
authors.lastname: Tiekink
authors.forename: Yogesh P
authors.initials: YP
authors.lastname: Patil
authors.forename: M
authors.initials: M
authors.lastname: Nethaji
authors.forename: Werner
authors.initials: W
authors.lastname: Kaminsky
authors.forename: Subhadip
authors.initials: S
authors.lastname: Mukhopadhyay
authors.forename: Sujit K
authors.initials: SK
authors.lastname: Bhutia
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D053837:DNA Cleavage; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D008672:Metals, Alkali / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D015394:Molecular Structure; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays; D014639:Vanadium / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D008024:Ligands; D008672:Metals, Alkali; D014639:Vanadium; D012710:Serum Albumin, Bovine; D004247:DNA