title: Changes in abundance of oral microbiota associated with oral cancer.
abstract: Individual bacteria and shifts in the composition of the microbiome have been associated with human diseases including cancer. To investigate changes in the microbiome associated with oral cancers, we profiled cancers and anatomically matched contralateral normal tissue from the same patient by sequencing 16S rDNA hypervariable region amplicons. In cancer samples from both a discovery and a subsequent confirmation cohort, abundance of Firmicutes (especially Streptococcus) and Actinobacteria (especially Rothia) was significantly decreased relative to contralateral normal samples from the same patient. Significant decreases in abundance of these phyla were observed for pre-cancers, but not when comparing samples from contralateral sites (tongue and floor of mouth) from healthy individuals. Weighted UniFrac principal coordinates analysis based on 12 taxa separated most cancers from other samples with greatest separation of node positive cases. These studies begin to develop a framework for exploiting the oral microbiome for monitoring oral cancer development, progression and recurrence.
authors.affiliation: Bluestone Center for Clinical Research, New York University College of Dentistry, New York, New York, United States of America; Department of Oral and Maxillofacial Surgery, New York University College of Dentistry, New York, New York, United States of America.
authors.forename: Brian L
authors.initials: BL
authors.lastname: Schmidt
authors.affiliation: Bioinformatics Department, Second Genome, San Bruno, California, United States of America.
authors.forename: Justin
authors.initials: J
authors.lastname: Kuczynski
authors.affiliation: Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, California, United States of America.
authors.forename: Aditi
authors.initials: A
authors.lastname: Bhattacharya
authors.affiliation: Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, California, United States of America.
authors.forename: Bing
authors.initials: B
authors.lastname: Huey
authors.affiliation: Bluestone Center for Clinical Research, New York University College of Dentistry, New York, New York, United States of America.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Corby
authors.affiliation: Bluestone Center for Clinical Research, New York University College of Dentistry, New York, New York, United States of America.
authors.forename: Erica L S
authors.initials: EL
authors.lastname: Queiroz
authors.affiliation: Bluestone Center for Clinical Research, New York University College of Dentistry, New York, New York, United States of America.
authors.forename: Kira
authors.initials: K
authors.lastname: Nightingale
authors.affiliation: Department of Oral and Maxillofacial Pathology, Radiology and Medicine, New York University College of Dentistry, New York, New York, United States of America.
authors.forename: A Ross
authors.initials: AR
authors.lastname: Kerr
authors.affiliation: Departments of Otolaryngology and Plastic Surgery, New York University, New York, New York, United States of America.
authors.forename: Mark D
authors.initials: MD
authors.lastname: DeLacure
authors.affiliation: Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, California, United States of America.
authors.forename: Ratna
authors.initials: R
authors.lastname: Veeramachaneni
authors.affiliation: Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, California, United States of America.
authors.forename: Adam B
authors.initials: AB
authors.lastname: Olshen
authors.affiliation: Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, California, United States of America.
authors.forename: Donna G
authors.initials: DG
authors.lastname: Albertson
mesh_terms: D016022:Case-Control Studies; D015331:Cohort Studies; D006801:Humans; D064307:Microbiota; D009055:Mouth / Q000382:microbiology*; D009062:Mouth Neoplasms / Q000382:microbiology*