title: Miscanthus: genetic diversity and genotype identification using ISSR and RAPD markers.
abstract: Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: inter simple sequence repeats (ISSRs), random amplified polymorphic DNA (RAPD), and by estimation of ploidy level based on flow cytometry. As a result, only one ISSR primer (ISSR1) and three RAPD primers (RAPD1, RAPD2, RAPD4) were required to identify all genotypes. Moreover, the use of the above mentioned molecular markers enable the proper species recognition of the interspecific hybrid M. × giganteus "Floridulus," which has been previously mislabeled as M. floridulus. The highest genetic similarity coefficient (0.94) was observed between M. × giganteus clones, which indicates that the genetic diversity within this species was very low. Whereas M. sinensis genotypes represented a relatively wide diversity with similarity coefficient of 0.58. Cluster analysis using UPGMA grouped the 18 accessions in three clusters according to species affiliation including relabeled M. × giganteus "Floridulus," which proved to be closely related to M.  × giganteus. Similar groupings were evident in the PCoA analysis.
authors.affiliation: Research Division in Bydgoszcz, Department of Genetics and Breeding of Root Crops, Plant Breeding and Acclimatization Institute - National Research Institute, Powstańców Wielkopolskich 10, 85-090, Bydgoszcz, Poland, s.cichorz@ihar.bydgoszcz.pl.
authors.forename: Sandra
authors.initials: S
authors.lastname: Cichorz
authors.forename: Maria
authors.initials: M
authors.lastname: Gośka
authors.forename: Anna
authors.initials: A
authors.lastname: Litwiniec
mesh_terms: D016000:Cluster Analysis; D018744:DNA, Plant / Q000235:genetics; D005434:Flow Cytometry; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D011003:Ploidies; D006109:Poaceae / Q000235:genetics*; D011110:Polymorphism, Genetic; D025341:Principal Component Analysis; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers