title: Salusin-β, an antimicrobially active peptide against Gram-positive bacteria.
abstract: Salusin-β has been detected in numerous mammalian tissues and has been shown to have various effects on the cardiovascular system. In this study, we showed that salusin-β exhibited potent antibacterial activity against Gram-positive microorganisms such as Bacillus subtilis NBRC 3513, Bacillus megaterium ATCC 19213, Staphylococcus aureus NBRC 12732, and Staphylococcus epidermidis NBRC 12933. A cytoplasmic membrane-depolarizing assay using the DiSC3(5) dye revealed that the addition of 4 nmol/mL of salusin-β caused the leakage of fluorescence dye from Staphylococcus aureus NBRC 12732. The antimicrobial potency and circular dichroism (CD) spectroscopy of five analogs related to salusin-β were examined to determine structure-function relationships in its N- and C-terminal regions. The results obtained suggest that the N-terminal sequences of the salusin-β molecule are important for the expression of the potent antimicrobial activity of this peptide. A profile corresponding to that of the α-helix conformation was observed in the salusin-β solution.
authors.affiliation: Department of Health and Nutrition, Osaka Shoin Women's University.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kimura
authors.forename: Mitsuno
authors.initials: M
authors.lastname: Shindo
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Moriizumi
authors.forename: Noriko
authors.initials: N
authors.lastname: Tagawa
authors.forename: Aya
authors.initials: A
authors.lastname: Fujinami
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kato
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Uchida
mesh_terms: D004305:Dose-Response Relationship, Drug; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011487:Protein Conformation; D013329:Structure-Activity Relationship
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; C478739:TOR2A protein, human