title: A Nonhost Peptidase Inhibitor of ~14 kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera.
abstract: Helicoverpa armigera is one of the major devastating pests of crop plants. In this context a serine peptidase inhibitor purified from the seeds of Butea monosperma was evaluated for its effect on developmental physiology of H. armigera larvae. B. monosperma peptidase inhibitor on 12% denaturing polyacrylamide gel electrophoresis exhibited a single protein band of ~14 kDa with or without reduction. In vitro studies towards total gut proteolytic enzymes of H. armigera and bovine trypsin indicated measurable inhibitory activity. B. monosperma peptidase inhibitor dose for 50% mortality and weight reduction by 50% were 0.5% w/w and 0.10% w/w, respectively. The IC50 of B. monosperma peptidase inhibitor against total H. armigera gut proteinases activity was 2.0 µg/mL. The larval feeding assays suggested B. monosperma peptidase inhibitor to be toxic as reflected by its retarded growth and development, consequently affecting fertility and fecundity of pest and prolonging the larval-pupal duration of the insect life cycle of H. armigera. Supplementing B. monosperma peptidase inhibitor in artificial diet at 0.1% w/w, both the efficiencies of conversion of ingested as well as digested food were downregulated, whereas approximate digestibility and metabolic cost were enhanced. The efficacy of Butea monosperma peptidase inhibitor against progressive growth and development of H. armigera suggest its usefulness in insect pest management of food crops.
authors.affiliation: Department of Biochemistry [DST-FIST & UGC-SAP Supported], Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.
authors.forename: Prabhash K
authors.initials: PK
authors.lastname: Pandey
authors.affiliation: Department of Biochemistry [DST-FIST & UGC-SAP Supported], Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.
authors.forename: Dushyant
authors.initials: D
authors.lastname: Singh
authors.affiliation: Department of Biochemistry [DST-FIST & UGC-SAP Supported], Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.
authors.forename: Sangram
authors.initials: S
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh 226025, India.
authors.forename: M Y
authors.initials: MY
authors.lastname: Khan
authors.affiliation: Department of Biochemistry [DST-FIST & UGC-SAP Supported], Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.
authors.forename: Farrukh
authors.initials: F
authors.lastname: Jamal