title: Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21.
abstract: A vector that drives the expression of the reporter gusA gene in both Lactobacillus plantarum and Lactococcus lactis was constructed in this study. This vector contained a newly characterized heat shock promoter (Phsp), amplified from an Enterococcus faecium plasmid, pAR6. Functionality and characterization of this promoter was initially performed by cloning Phsp into pNZ8008, a commercial lactococcal plasmid used for screening of putative promoters which utilizes gusA as a reporter. It was observed that Phsp was induced under heat, salinity and alkaline stresses or a combination of all three stresses. The newly characterized Phsp promoter was then used to construct a novel Lactobacillus vector, pAR1801 and its ability to express the gusA under stress-induced conditions was reproducible in both Lb. plantarum Pa21 and L. lactis M4 hosts.
authors.affiliation: Department of Cell and Molecular Biology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mohd Shawal Thakib
authors.initials: MS
authors.lastname: Maidin
authors.affiliation: Department of Cell and Molecular Biology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Adelene Ai-Lian
authors.initials: AA
authors.lastname: Song
authors.affiliation: Department of Cell and Molecular Biology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Tannaz
authors.initials: T
authors.lastname: Jalilsood
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Institute of Bioscience Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Chin Chin
authors.initials: CC
authors.lastname: Sieo
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Institute of Bioscience Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Khatijah
authors.initials: K
authors.lastname: Yusoff
authors.affiliation: Department of Cell and Molecular Biology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Institute of Bioscience Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: raha@upm.edu.my.
authors.forename: Raha Abdul
authors.initials: RA
authors.lastname: Rahim
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics*; D048191:Lactiplantibacillus plantarum / Q000235:genetics*; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013312:Stress, Physiological / Q000235:genetics
keywords: Heat shock promoter; LAB; Lactobacillus plantarum; Lactococcus lactis