title: A cytosolic glutathione s-transferase, GST-theta from freshwater prawn Macrobrachium rosenbergii: molecular and biochemical properties.
abstract: Glutathione S-transferases play an important role in cellular detoxification and may have evolved to protect cells against reactive oxygen metabolites. In this study, we report the molecular characterization of glutathione s-transferase-theta (GST-θ) from freshwater prawn Macrobrachium rosenbergii. A full length cDNA of GSTT (1417 base pairs) was isolated and characterized bioinformatically. Exposure to virus (white spot syndrome baculovirus or M. rosenbergii nodovirus), bacteria (Aeromonas hydrophila or Vibrio harveyi) or heavy metals (cadmium or lead) significantly increased the expression of GSTT (P<0.05) in hepatopancreas. Recombinant GST-θ with monochlorobimane substrate had an optimum activity at pH7.5 and 35 °C. Furthermore recombinant GST-θ activity was abolished by the denaturants triton X-100, Gua-HCl, Gua-thiocyanate, SDS and urea in a dose-dependent manner. Overall, the results suggest a potential role for M. rosenbergii GST-θ in detoxification and possibly conferring immune protection.
authors.affiliation: Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur 603 203, Chennai, Tamil Nadu, India. Electronic address: jesuaraj@hotmail.com.
authors.forename: Jesu
authors.initials: J
authors.lastname: Arockiaraj
authors.affiliation: Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A4800, Austin, TX 78712, USA.
authors.forename: Annie J
authors.initials: AJ
authors.lastname: Gnanam
authors.affiliation: Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur 603 203, Chennai, Tamil Nadu, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Palanisamy
authors.affiliation: Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur 603 203, Chennai, Tamil Nadu, India.
authors.forename: Prasanth
authors.initials: P
authors.lastname: Bhatt
authors.affiliation: Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur 603 203, Chennai, Tamil Nadu, India.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Kumaresan
authors.affiliation: Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur 603 203, Chennai, Tamil Nadu, India.
authors.forename: Mukesh Kumar
authors.initials: MK
authors.lastname: Chaurasia
authors.affiliation: Lab PCN 206, Microbiology Division, CSIR-Central Drug Research Institute, B.S. 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, Uttar Pradesh, India.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Pasupuleti
authors.affiliation: PG and Research Department of Biotechnology, Bharath College of Science and Management, Thanjavur 613 005, Tamil Nadu, India.
authors.forename: Harikrishnan
authors.initials: H
authors.lastname: Ramaswamy
authors.affiliation: Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur 603 203, Chennai, Tamil Nadu, India; Department of Microbiology, SRM Arts & Science College, Kattankulathur 603 203, Chennai, India.
authors.forename: Abirami
authors.initials: A
authors.lastname: Arasu
authors.affiliation: Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur 603 203, Chennai, Tamil Nadu, India; Department of Biotechnology, SRM Arts & Science College, Kattankulathur 603 203, Chennai, India.
authors.forename: Akila
authors.initials: A
authors.lastname: Sathyamoorthi
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology; D000818:Animals; D060829:Arthropod Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D002104:Cadmium / Q000633:toxicity; D018076:DNA, Complementary; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology*; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D043143:Hepatopancreas / Q000201:enzymology / Q000276:immunology / Q000821:virology; D007854:Lead / Q000633:toxicity; D033541:Palaemonidae / Q000201:enzymology* / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000821:virology; D046848:White spot syndrome virus 1 / Q000276:immunology
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D002104:Cadmium; D007854:Lead; D005982:Glutathione Transferase
keywords: Enzyme activity; Gene expression; Glutathione S-transferase; Macrobrachium rosenbergii; Oxidative stress