title: Concurrent administration of an intranasal vaccine containing feline herpesvirus-1 (FHV-1) with a parenteral vaccine containing FHV-1 is superior to parenteral vaccination alone in an acute FHV-1 challenge model.
abstract: The administration of intranasal (IN) or subcutaneous (SC) vaccines containing modified live feline herpesvirus 1 (FHV-1) offers some level of protection against FHV-1 challenge, but relative efficacy is <100%. In this study, clinical signs and viral shedding in kittens were compared among three groups: (1) kittens vaccinated concurrently with IN and SC vaccines containing FHV-1 (Group 1, n = 8); (2) kittens vaccinated with a SC FHV-1 vaccine alone (Group 2, n = 8), and (3) unvaccinated control kittens (Group 3, n = 8). All kittens were FHV-1 naïve at enrolment, and challenge with a virulent strain of FHV-1 was performed 1 week after vaccination. Daily clinical signs and pharyngeal FHV-1 shedding were recorded over a 21-day infection period. Overall, kittens in Group 1 had significantly less severe clinical illness than those in Group 2 (P < 0.05). Additionally, significantly less FHV-1 DNA was detected on pharyngeal swabs from kittens in Group 1 compared to those in Group 2 (P < 0.001). Concomitant administration of IN and SC FHV-1 vaccines was superior to administration of the SC FHV-1 vaccine alone in this challenge model of FHV-1 naïve kittens.
authors.affiliation: The Center for Companion Animal Studies, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: krystle.reagan@colostate.edu.
authors.forename: K L
authors.initials: KL
authors.lastname: Reagan
authors.affiliation: The Center for Companion Animal Studies, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Hawley
authors.affiliation: The Center for Companion Animal Studies, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Lappin
mesh_terms: D000281:Administration, Intranasal / Q000662:veterinary; D000818:Animals; D002371:Cat Diseases / Q000628:therapy* / Q000821:virology; D002415:Cats; D005260:Female; D006566:Herpesviridae Infections / Q000628:therapy / Q000662:veterinary* / Q000821:virology; D022283:Herpesvirus Vaccines / Q000008:administration & dosage / Q000494:pharmacology*; D007279:Injections, Subcutaneous / Q000662:veterinary; D008297:Male; D018140:Varicellovirus / Q000276:immunology / Q000502:physiology*; D017201:Virus Shedding
chemical_list: D022283:Herpesvirus Vaccines
keywords: Feline herpesvirus 1; Intranasal; Polymerase chain reaction; Subcutaneous; Vaccine