title: A detailed three-step protocol for live imaging of intracellular traffic in polarized primary porcine RPE monolayers.
authors.affiliation: Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA; McPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Toops
authors.affiliation: Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA; Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: Li Xuan
authors.initials: LX
authors.lastname: Tan
authors.affiliation: Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA; McPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, USA; Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA. Electronic address: lakkaraju@wisc.edu.
authors.forename: Aparna
authors.initials: A
authors.lastname: Lakkaraju
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D016764:Cell Polarity; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D007424:Intracellular Fluid; D008297:Male; D015203:Reproducibility of Results; D012162:Retinal Degeneration / Q000378:metabolism / Q000473:pathology*; D055213:Retinal Pigment Epithelium / Q000378:metabolism* / Q000473:pathology; D013552:Swine
keywords: age-related macular degeneration; culture protocol; gene expression; live imaging; organelle traffic; polarized monolayers; retinal diseases; retinal pigment epithelium