title: Caryophyllene sesquiterpenes from the marine-derived fungus Ascotricha sp. ZJ-M-5 by the one strain-many compounds strategy.
abstract: The marine-derived fungus Ascotricha sp. ZJ-M-5, which can produce cyclonerodiol analogues, a 3,4-seco lanostane triterpenoid, and diketopiperazines in an eutrophic medium, was subjected to a one strain-many compounds (OSMAC) analysis. It was found to produce three new caryophyllene derivatives (1-3) and the known 1,3,6-trihydroxy-8-methylxanthone (4) in an oligotrophic medium, Czapek Dox broth with or without Mg(2+). (+)-6-O-Demethylpestalotiopsin A (1) and (+)-6-O-demethylpestalotiopsin C (2), which have a five-membered hemiacetal structural moiety, showed growth inhibitory effects against HL-60 and K562 leukemia cell lines with the lowest GI50 value of 6.9 ± 0.4 μM. It can be concluded that modification of the culture media is still effective in the discovery of novel bioactive fungal secondary metabolites.
authors.affiliation: Key Laboratory of Structure-Based Drug Design & Discovery, Shenyang Pharmaceutical University, Ministry of Education , Shenyang 110016, People's Republic of China.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Wang
authors.forename: Dan-Yi
authors.initials: DY
authors.lastname: Li
authors.forename: Yan-Chun
authors.initials: YC
authors.lastname: Li
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Hua
authors.forename: En-Long
authors.initials: EL
authors.lastname: Ma
authors.forename: Zhan-Lin
authors.initials: ZL
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D054659:Diketopiperazines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D020014:K562 Cells; D008386:Marine Biology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013098:Xylariales / Q000737:chemistry*
chemical_list: C000590852:6-O-demethylpestalotiopsin A; C000590853:6-O-demethylpestalotiopsin B; C000590854:6-O-demethylpestalotiopsin C; D054659:Diketopiperazines; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C000539:cyclonerodiol; C024714:caryophyllene