title: Gene delivery from supercharged coiled-coil protein and cationic lipid hybrid complex.
abstract: A lipoproteoplex comprised of an engineered supercharged coiled-coil protein (CSP) bearing multiple arginines and the cationic lipid formulation FuGENE HD (FG) was developed for effective condensation and delivery of nucleic acids. The CSP was able to maintain helical structure and self-assembly properties while exhibiting binding to plasmid DNA. The ternary CSP·DNA(8:1)·FG lipoproteoplex complex demonstrated enhanced transfection of β-galactosidase DNA into MC3T3-E1 mouse preosteoblasts. The lipoproteoplexes showed significant increases in transfection efficiency when compared to conventional FG and an mTat·FG lipopolyplex with a 6- and 2.5-fold increase in transfection, respectively. The CSP·DNA(8:1)·FG lipoproteoplex assembled into spherical particles with a net positive surface charge, enabling efficient gene delivery. These results support the application of lipoproteoplexes with protein engineered CSP for non-viral gene delivery.
authors.affiliation: Department of Chemical and Biomolecular Engineering, New York University Polytechnic School of Engineering, 6 Metrotech Center, Brooklyn, NY 11201, USA.
authors.forename: Haresh T
authors.initials: HT
authors.lastname: More
authors.affiliation: Department of Chemical and Biomolecular Engineering, New York University Polytechnic School of Engineering, 6 Metrotech Center, Brooklyn, NY 11201, USA.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Frezzo
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Jisen
authors.initials: J
authors.lastname: Dai
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Yamano
authors.affiliation: Department of Chemical and Biomolecular Engineering, New York University Polytechnic School of Engineering, 6 Metrotech Center, Brooklyn, NY 11201, USA. Electronic address: montclare@nyu.edu.
authors.forename: Jin K
authors.initials: JK
authors.lastname: Montclare
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002412:Cations; D002470:Cell Survival / Q000502:physiology; D002942:Circular Dichroism; D053938:DNA Fragmentation; D017931:DNA Primers / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D008055:Lipids / Q000737:chemistry; D008074:Lipoproteins / Q000737:chemistry; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding; D015202:Protein Engineering; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D002412:Cations; D017931:DNA Primers; C411955:FuGene; D008055:Lipids; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D011108:Polymers; D001616:beta-Galactosidase
keywords: Cationic lipid; Coiled-coil protein; Gene delivery; Lipoproteoplexes; Supercharge