title: BMP2-encapsulated chitosan coatings on functionalized Ti surfaces and their performance in vitro and in vivo.
abstract: Bone morphogenic protein-2 (BMP2)-encapsulated chitosan (CS) coatings were prepared to immobilize BMP2 on titanium (Ti) surfaces. The Ti substrates were functionalized through a three-step process: alkali treatment, silanization with 3-aminopropyltriethoxysilane and aldehydation with glutaraldehyde (GA). BMP2-encapsulated CS coatings (BMP2-CS) were bonded to Ti surfaces through reactions between the aldehyde groups of GA and the amine groups of CS. Direct BMP2 immobilization on aldehyde-treated Ti (BMP2-Ti) and pure CS coatings (CS-Ti) were used as controls. The release rate of BMP2-CS-Ti was half of that of BMP2-Ti at initial stage, which indicates that the CS coatings are suitable carriers for sustained BMP2 release. The osteoinductivities of BMP2-CS-Ti, BMP2-Ti, CS-Ti and pristine Ti were examined by both in vitro cell tests and in vivo experiments. Bone marrow stem cell (BMSC) culture indicated that BMP2-CS-Ti is more potent in stimulating the differentiation of the adhering BMSC than the three other groups. Rabbit femur implantation revealed the excellent osteoinductivity of BMP2-CS-coated Ti implants. These results demonstrate that the BMP2-encapsulated CS coatings are stable osteoinductive coatings that realize the sustained release of BMP2 and maintain the activity of the protein.
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, China. Electronic address: luxiong_2004@163.com.
authors.forename: Xiong
authors.initials: X
authors.lastname: Lu
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhi
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Ke-Feng
authors.initials: KF
authors.lastname: Wang
authors.affiliation: The Second People's Hospital of Neijiang, Neijiang, Sichuan, China.
authors.forename: Fan-Zhi
authors.initials: FZ
authors.lastname: Meng
authors.affiliation: The Second People's Hospital of Neijiang, Neijiang, Sichuan, China.
authors.forename: Ou
authors.initials: O
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry* / Q000378:metabolism; D018786:Bone Substitutes / Q000737:chemistry* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D005269:Femur / Q000187:drug effects / Q000473:pathology; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D011437:Propylamines; D019736:Prostheses and Implants; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D055767:Immobilized Proteins; D011437:Propylamines; D012821:Silanes; D048271:Chitosan; D014025:Titanium; C477625:amino-propyl-triethoxysilane; D005976:Glutaral
keywords: BMP2; Chitosan coatings; Functionalization Ti; Osteoinductivity