title: Detection of circulating tumor cells using targeted surface-enhanced Raman scattering nanoparticles and magnetic enrichment.
abstract: While more than 90% of cancer deaths are due to metastases, our ability to detect circulating tumor cells (CTCs) is limited by low numbers of these cells in the blood and factors confounding specificity of detection. We propose a magnetic enrichment and detection technique for detecting CTCs with high specificity. We targeted both magnetic and surface-enhanced Raman scattering (SERS) nanoparticles to cancer cells. Only cells that are dual-labeled with both kinds of nanoparticles demonstrate an increasing SERS signal over time due to magnetic trapping.
authors.affiliation: University of Alberta, Department of Electrical and Computer Engineering, 9107, 116 Street, Edmonton T6G 2V4, Canada.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: University of Alberta, Department of Electrical and Computer Engineering, 9107, 116 Street, Edmonton T6G 2V4, Canada.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Paproski
authors.affiliation: University of Alberta, Cross Cancer Institute, Department of Oncology, 11560 University Avenue, Edmonton T6G 1Z2, Canada.
authors.forename: Ronald
authors.initials: R
authors.lastname: Moore
authors.affiliation: University of Alberta, Department of Electrical and Computer Engineering, 9107, 116 Street, Edmonton T6G 2V4, Canada.
authors.forename: Roger
authors.initials: R
authors.lastname: Zemp
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D005492:Folic Acid / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008954:Models, Biological; D009360:Neoplastic Cells, Circulating / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D058185:Magnetite Nanoparticles; D005492:Folic Acid