title: DHTP is an allosteric inhibitor of the kinesin-13 family of microtubule depolymerases.
abstract: The kinesin-13 family of microtubule depolymerases is a major regulator of microtubule dynamics. RNA interference-induced knockdown studies have highlighted their importance in many cell division processes including spindle assembly and chromosome segregation. Since microtubule turnovers and most mitotic events are relatively rapid (in minutes or seconds), developing tools that offer faster control over protein functions is therefore essential to more effectively interrogate kinesin-13 activities in living cells. Here, we report the identification and characterization of a selective allosteric kinesin-13 inhibitor, DHTP. Using high resolution microscopy, we show that DHTP is cell permeable and can modulate microtubule dynamics in cells.
authors.affiliation: Chemical Biology of Cell Division Laboratory, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montréal, Québec H3C 3J7, Canada.
authors.forename: Lama
authors.initials: L
authors.lastname: Talje
authors.affiliation: Cellular Mechanisms of Morphogenesis during Mitosis and Cell Motility Laboratory, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montréal, Québec H3C 3J7, Canada.
authors.forename: Khaled
authors.initials: K
authors.lastname: Ben El Kadhi
authors.affiliation: Chemical Biology of Cell Division Laboratory, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montréal, Québec H3C 3J7, Canada.
authors.forename: Kaleem
authors.initials: K
authors.lastname: Atchia
authors.affiliation: Chemical Biology of Cell Division Laboratory, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montréal, Québec H3C 3J7, Canada.
authors.forename: Thierry
authors.initials: T
authors.lastname: Tremblay-Boudreault
authors.affiliation: Cellular Mechanisms of Morphogenesis during Mitosis and Cell Motility Laboratory, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montréal, Québec H3C 3J7, Canada; Département de Pathologie et de Biologie Cellulaire, Université de Montréal, Montréal, Québec H3C 3J7, Canada.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Carreno
authors.affiliation: Chemical Biology of Cell Division Laboratory, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montréal, Québec H3C 3J7, Canada; Département de médecine, Université de Montréal, Montréal, Québec H3C 3J7, Canada. Electronic address: benjamin.kwok@umontreal.ca.
authors.forename: Benjamin H
authors.initials: BH
authors.lastname: Kwok
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D000494:Allosteric Regulation; D000818:Animals; D002417:Cattle; D004353:Drug Evaluation, Preclinical; D006801:Humans; D016547:Kinesins / Q000037:antagonists & inhibitors* / Q000737:chemistry; D008870:Microtubules / Q000737:chemistry; D055503:Protein Multimerization; D011743:Pyrimidines / Q000737:chemistry*; D053778:Thiazolidines / Q000737:chemistry*; D050257:Tubulin Modulators / Q000737:chemistry*
chemical_list: C000590562:2-(4-((5-(4-chlorophenyl)-6-(isopropoxycarbonyl)-7-methyl-3-oxo-3,5-dihydro-2H-thiazolo(3,2-a)pyrimidin-2-ylidene)methyl)phenoxy)acetic acid; C417670:KIF13A protein, human; D011743:Pyrimidines; D053778:Thiazolidines; D050257:Tubulin Modulators; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D016547:Kinesins
keywords: Cell division; Chemical inhibitor; Enzyme; Kinesin; Microtubule