title: Assessing regulated nuclear transport in Saccharomyces cerevisiae.
abstract: Regulated protein transport between the cytoplasm and the nucleoplasm occurs through nuclear pore complexes and is critical to the function of numerous biological pathways. Saccharomyces cerevisiae has been used as a model system to probe the underlying mechanisms of nuclear transport and how they regulate various physiological processes. This has been facilitated, in part, by studies that couple the microscopic observation of a fluorescently tagged transport cargo's in vivo localization with numerous genetic and biochemical tools available to yeast researchers. Here, we describe some of these methods as they pertain to studies on regulated nuclear transport.
authors.affiliation: Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Christopher
authors.initials: C
authors.lastname: Ptak
authors.affiliation: Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Wozniak
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics*; D020816:Amino Acid Motifs; D059447:Cell Cycle Checkpoints / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D049452:Green Fluorescent Proteins; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism; D028884:Karyopherins / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013696:Temperature; D028901:alpha Karyopherins / Q000378:metabolism; D028961:beta Karyopherins / Q000378:metabolism; D000097606:Exportin 1 Protein
chemical_list: D049452:Green Fluorescent Proteins; D020662:Guanine Nucleotide Exchange Factors; D028884:Karyopherins; D026901:Membrane Transport Proteins; D020559:Monomeric GTP-Binding Proteins; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins; D028901:alpha Karyopherins; D028961:beta Karyopherins; D000097606:Exportin 1 Protein; C080324:GSP1 protein, S cerevisiae; C483822:PSE1 protein, S cerevisiae; C067050:SRM1 protein, S cerevisiae
keywords: Cell cycle; Karyopherins; Nuclear pore complex; Saccharomyces cerevisiae; nuclear transport