title: Operational stability of naringinase PVA lens-shaped microparticles in batch stirred reactors and mini packed bed reactors-one step closer to industry.
abstract: The immobilization of naringinase in PVA lens-shaped particles, a cheap and biocompatible hydrogel was shown to provide an effective biocatalyst for naringin hydrolysis, an appealing reaction in the food and pharmaceutical industries. The present work addresses the operational stability and scale-up of the bioconversion system, in various types of reactors, namely shaken microtiter plates (volume ⩽ 2 mL), batch stirred tank reactors (volume <400 mL) and a packed-bed reactor (PBR, 6.8 mL). Consecutive batch runs were performed with the shaken/stirred vessels, with reproducible and encouraging results, related to operational stability. The PBR was used to establish the feasibility for continuous operation, running continuously for 54 days at 45°C. The biocatalyst activity remained constant for 40 days of continuous operation. The averaged specific productivity was 9.07 mmol h(-1) g enzyme(-1) and the half-life of 48 days.
authors.affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: Mário A P
authors.initials: MA
authors.lastname: Nunes
authors.affiliation: Instituto de Ciência e Engenharia de Materiais e Superficies (ICEMS), Instituto Superior Técnico, Universidade Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
authors.forename: M Emilia
authors.initials: ME
authors.lastname: Rosa
authors.affiliation: Institute for Biotechnology and Bioengineering (IBB), Centre for Biological and Chemical Engineering, Department of Bioengineering, Instituto Superior Técnico, Universidade Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal; Faculdade de Engenharia, Universidade Lusófona de Humanidades e Tecnologias, 1749-024 Lisboa, Portugal.
authors.forename: Pedro C B
authors.initials: PC
authors.lastname: Fernandes
authors.affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal. Electronic address: mhribeiro@ff.ul.pt.
authors.forename: Maria H L
authors.initials: MH
authors.lastname: Ribeiro
mesh_terms: D061249:Batch Cell Culture Techniques / Q000295:instrumentation*; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis; D007221:Industry; D008863:Microspheres; D009097:Multienzyme Complexes / Q000378:metabolism*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D012212:Rheology; D013997:Time Factors; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D009097:Multienzyme Complexes; D011142:Polyvinyl Alcohol; C039827:naringinase; D001617:beta-Glucosidase
keywords: (Mini)bioreactors; Naringinase entrapment; Operational stability; PBR-packed-bed reactor; PVA-poly(vinyl alcohol)