title: Mining the pre-diagnostic antibody repertoire of TgMMTV-neu mice to identify autoantibodies useful for the early detection of human breast cancer.
abstract: BACKGROUND
  The use of autoantibodies for the early detection of breast cancer has generated much interest as antibodies can be readily assayed in serum when antigen levels are low. Ideally, diagnostic autoantibodies would be identified in individuals who harbored pre-invasive disease/high risk lesions leading to malignancy. Prospectively collected human serum samples from these individuals are rare and not often available for biomarker discovery. We questioned whether transgenic animals could be used to identify cancer-associated autoantibodies present at the earliest stages of the malignant transformation of breast cancer.
  
  
  METHODS
  We collected sera from transgenic mice (TgMMTV-neu) from the time of birth to death by spontaneous mammary tumors. Using sera from a time point prior to the development of tumor, i.e. "pre-diagnostic", we probed cDNA libraries derived from syngeneic tumors to identify proteins recognized by IgG antibodies. Once antigens were identified, selected proteins were evaluated via protein arrays, for autoantibody responses using plasma from women obtained prior to the development of breast cancer and matched controls. The ability of the antigens to discriminate cases from controls was assessed using receiver-operating-characteristic curve analyses and estimates of the area under the curve.
  
  
  RESULTS
  We identified 6 autoantibodies that were present in mice prior to the development of mammary cancer: Pdhx, Otud6b, Stk39, Zpf238, Lgals8, and Vps35. In rodent validation cohorts, detecting both IgM and IgG antibody responses against a subset of the identified proteins could discriminate pre-diagnostic sera from non-transgenic control sera with an AUC of 0.924. IgG and IgM autoantibodies, specific for a subset of the identified antigens, could discriminate the samples of women who eventually developed breast cancer from case-matched controls who did not develop disease. The discriminatory potential of the pre-diagnostic autoantibodies was enhanced if plasma samples were collected greater than 5 months prior to a breast cancer diagnosis (AUC 0.68; CI 0.565-0.787, p=0.0025).
  
  
  CONCLUSIONS
  Genetically engineered mouse models of cancer may provide a facile discovery tool for identifying autoantibodies useful for human cancer diagnostics.
authors.forename: Jianning
authors.initials: J
authors.lastname: Mao
authors.forename: Jon
authors.initials: J
authors.lastname: Ladd
authors.forename: Ekram
authors.initials: E
authors.lastname: Gad
authors.forename: Lauren
authors.initials: L
authors.lastname: Rastetter
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Johnson
authors.forename: Edmond
authors.initials: E
authors.lastname: Marzbani
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Childs
authors.forename: Hailing
authors.initials: H
authors.lastname: Lu
authors.forename: Yushe
authors.initials: Y
authors.lastname: Dang
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Broussard
authors.forename: Sasha E
authors.initials: SE
authors.lastname: Stanton
authors.forename: Sam M
authors.initials: SM
authors.lastname: Hanash
authors.affiliation: Tumor Vaccine Group, Center for Translational Medicine in Women's Health, University of Washington, 850 Republican Street, Box 358050, Seattle, WA 98109, USA. ndisis@uw.edu.
authors.forename: Mary L
authors.initials: ML
authors.lastname: Disis
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology*; D001943:Breast Neoplasms / Q000175:diagnosis*; D042241:Early Diagnosis; D005260:Female; D018734:Genes, erbB-2; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D001323:Autoantibodies; D014408:Biomarkers, Tumor