title: [Expression level of SET gene in acute myeloid leukemia and its clinical significance].
abstract: OBJECTIVE
  To investigate the expression level of SET gene in patients with acute myeloid leukemia (AML) and evaluate its significance.
  
  
  METHODS
  The expression level of SET gene in 141 de novo AML patients was determined by real time quantitative PCR (RQ-PCR), and its relationship with the clinical features and outcomes of these patients were analyzed.
  
  
  RESULTS
  SET gene transcript level was detected in 141 AML patients with the median expression level of 0.86(range 0.02-15.69). AML patients with higher SET gene expression had a higher level of white blood cell (WBC ≥ 100 × 10⁹/L) count than of lower SET gene expression ones (31.0% vs 11.4%, P=0.005). In the 136 patients who received treatment after diagnosis, higher SET gene expression group had lower complete remission rate (50.0%) than of lower expression cohort (73.5%) after two cycles of chemotherapy (P=0.005). Survival analysis showed that patients with higher SET gene expression had significantly shorter overall survival(OS) (10 months vs 22 months, P=0.001) and event-free survival (EFS) (2 months vs 14 months, P=0.005) than of lower SET gene expression ones. Multivariate COX regression analysis showed SET overexpression was an independent prognostic factor for OS. In the patients with the normal karyotype, higher SET expression group also had significantly shorter OS (12 months vs 35 months, P=0.010) and EFS (4 months vs 14 months, P=0.026) than of lower SET expression ones.
  
  
  CONCLUSIONS
  High expression of SET gene was associated with poor prognosis and might be a prognostic molecular marker of AML.
authors.forename: Peipei
authors.initials: P
authors.lastname: Ye
authors.affiliation: Department of Hematology, Institute of Hematology, Zhejiang University; Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Mengxia
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Hematology, Institute of Hematology, Zhejiang University; Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Qitian
authors.initials: Q
authors.lastname: Mu
authors.affiliation: Department of Hematology, Institute of Hematology, Zhejiang University; Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Chen
authors.forename: Renzhi
authors.initials: R
authors.lastname: Pei
authors.affiliation: Department of Hematology, Institute of Hematology, Zhejiang University; Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Hematology, Institute of Hematology, Zhejiang University; Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Lou
authors.affiliation: Department of Hematology, Institute of Hematology, Zhejiang University; Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Qian
authors.affiliation: Department of Hematology, Institute of Hematology, Zhejiang University; Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Meng
authors.affiliation: Department of Hematology, Institute of Hematology, Zhejiang University; Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Tong
authors.affiliation: Department of Hematology, Institute of Hematology, Zhejiang University; Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Mai
authors.affiliation: Department of Hematology, Institute of Hematology, Zhejiang University; Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Huanping
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Hematology, Institute of Hematology, Zhejiang University; Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
mesh_terms: D004268:DNA-Binding Proteins; D018572:Disease-Free Survival; D015972:Gene Expression Regulation, Neoplastic; D056488:Histone Chaperones / Q000235:genetics*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D011379:Prognosis; D012074:Remission Induction; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D056488:Histone Chaperones; C513473:SET protein, human; D014157:Transcription Factors