title: [Effects of recombinant human interleukin-11 on LPS-induced intestinal epithelial cell injury in rats].
abstract: OBJECTIVE
  To study the effects of recombinant human interleukin-11 (rhIL-11) on the proliferation and apoptosis of rat intestinal epithelial cell line (IEC-6).
  
  
  METHODS
  IEC-6 cells were treated with LPS to establish necrotizing enterocolitis (NEC) model in vitro. rhIL-11 (100 ng/mL) was administered following LPS treatment and these cells were used as the IL-11 treatment group. The cells treated with normal saline only served as the control group. MTT assay was used to determine an optimal concentration (5-200 μg/mL) and time (1-24 h). MTT assay was used to measure the proliferation of IEC-6 cells at 3, 6, 9 and 12 hours after rhIL-11 treatment. Flow cytometry was used to evaluate the apoptosis of IEC-6 cells.
  
  
  RESULTS
  IEC-6 cells treated with various concentrations of LPS at various time points showed a lower proliferation than the control group (P<0.05). After 9 hours of rhIL-11 treatment, the proliferation activity of IEC-6 cells in the IL-11 treatment group significantly increased compared with the NEC model group without rhIL-11 treatment (P<0.05), reaching to the level of the control group. The total apoptotic and necrotic rate of IEC-6 cells in the IL-11 treatment group decreased significantly compared with the NEC model group without rhIL-11 treatment (P<0.01), but were still higher than the control group (P<0.05).
  
  
  CONCLUSIONS
  rhIL-11 can promote proliferation and reduce apoptotic and necrotic rates of IEC-6 cells treated with LPS.
authors.affiliation: Very Premature Infants Intensive Care Unit, Bayi Children's Hospital Affiliated to General Hospital of Beijing Military Command, Beijing 100700, China. wangrjok@163.com.
authors.forename: Na
authors.initials: N
authors.lastname: Cai
authors.forename: Zhi-Chun
authors.initials: ZC
authors.lastname: Feng
authors.forename: Rui-Juan
authors.initials: RJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D017370:Interleukin-11 / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009336:Necrosis; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D017370:Interleukin-11; D008070:Lipopolysaccharides; D011994:Recombinant Proteins