title: Screening of transgenic frost-resistant cotton using a porous silicon biosensing platform.
abstract: BACKGROUND
  The genetic screening of exogenous gene expression is critical for the transgenic plant breeding.
  
  
  OBJECTIVE
  Here, a novel label-free porous silicon (PSi)-based biosensor is reported for the identification of frost-resistant cotton.
  
  
  METHODS
  Changes in optical response signal in the presence of antifreeze protein (AFP) were detected by Fourier transform infrared (FTIR) spectromicroscopy when binding of the target AFP protein with anti-AFP-antibodies was selectively captured on the PSi biosensor.
  
  
  RESULTS
  Compared with non-transgenic plants, significant red shifts were observed for transgenic frost-resistant cotton lines except a transgenic line 2.
  
  
  CONCLUSIONS
  The approach is highly efficient with lower cost and high sensitivity for detecting large number of transgenic samples in the trial field simultaneously. This PSi biosensing platform has potential commercial applications for the rapid assessment of transgenic plants in the field.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Jieqiong
authors.initials: J
authors.lastname: Li
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Lv
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Jia
authors.forename: Ji
authors.initials: J
authors.lastname: Ma
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000906:Antibodies / Q000737:chemistry; D021301:Antifreeze Proteins / Q000032:analysis* / Q000235:genetics; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003080:Cold Temperature; D015870:Gene Expression; D003368:Gossypium / Q000235:genetics*; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D030821:Plants, Genetically Modified; D016062:Porosity; D011485:Protein Binding; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000906:Antibodies; D021301:Antifreeze Proteins; D010940:Plant Proteins; D012825:Silicon