title: Inhibition of GPR137 expression reduces the proliferation and colony formation of malignant glioma cells.
abstract: GPR137 are ubiquitously expressed in the central nervous system. However, the role o f GPR137 in human malignant glioma is still poorly known. In the present study, we firstly detected the expression of GPR137 in 29 human glioma tissue specimens by immunohistochemistry and in 5 malignant glioma cell lines by quantitative RT-PCR. The expression of GPR137 was much stronger in high-grade gliomas than in low-grade gliomas. Lentivirus-mediated small interfering RNAs (siRNAs) were employed to knock down GPR137 expression in glioma cells. Inhibition of GPR137 expression by RNAi significantly inhibited the proliferation and colony-forming capacity of U251, A172 and U373 cells. Moreover, flow cytometry analysis showed that knockdown of GPR137 led to the cell-cycle arrest at the S phase. Our results indicated that GPR137 is involved in the progression of human glioma, suggesting GPR137 as a potential oncogene of glioma cells.
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Anhui Medical University, and Cerebral Vascular Disease Research Center, Anhui Medical University, 678 Fu Rong Road, Hefei, 230601, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zong
authors.forename: Hongliang
authors.initials: H
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Xie
authors.forename: Erbao
authors.initials: E
authors.lastname: Bian
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
mesh_terms: D015153:Blotting, Western; D001932:Brain Neoplasms / Q000473:pathology*; D059447:Cell Cycle Checkpoints / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D043562:Receptors, G-Protein-Coupled