title: The benefits of hypothermic machine perfusion are enhanced with Vasosol and α-tocopherol in rodent donation after cardiac death livers.
abstract: The use of hypothermic machine perfusion (HMP) has recently been used to show an improvement in both standard and extended criteria donor liver grafts but creating a more dynamic preservation environment that can be supplemented with a variety of additives to aid in cold temperature metabolism and vasodilatation. Increasing the benefits of HMP, we explore the use of α-tocopherol in reducing inflammatory markers and apoptotic pathways to reduce the incidence of preservation injury. We explored the use of a donation after cardiac death (DCD) rodent model to test the additive benefits of α-tocopherol in HMP. The addition of α-tocopherol reduced the level of alanine aminotransferase (ALT) over the course of reperfusion as well, reduced the levels of inflammatory cytokines within a 90 minute reperfusion biopsy. Further benefit was seen with α-tocopherol through the reduction of the level of caspase 3/7 in the circulation, shown to be a result of the reduction of the levels of Cytochrome C mRNA. Liver perfusion with Vasosol® and HMP could benefit further from the addition of α-tocopherol to existing formulations of Vasosol®.
authors.affiliation: Center for Liver Disease and Transplantation, Department of Surgery, Columbia University Medical Center, New York, New York, USA.
authors.forename: C
authors.initials: C
authors.lastname: Bae
authors.affiliation: Center for Liver Disease and Transplantation, Department of Surgery, Columbia University Medical Center, New York, New York, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Pichardo
authors.affiliation: Columbia Biomedical Engineering, The Fu Foundation School of Engineering and Applied Science, Columbia University, New York, New York, USA.
authors.forename: H
authors.initials: H
authors.lastname: Huang
authors.affiliation: Center for Liver Disease and Transplantation, Department of Surgery, Columbia University Medical Center, New York, New York, USA.
authors.forename: S D
authors.initials: SD
authors.lastname: Henry
authors.affiliation: Center for Liver Disease and Transplantation, Department of Surgery, Columbia University Medical Center, New York, New York, USA. Electronic address: jjg46@columbia.edu.
authors.forename: J V
authors.initials: JV
authors.lastname: Guarrera
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D045304:Cytochromes c / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D017931:DNA Primers; D003643:Death; D005260:Female; D007036:Hypothermia, Induced; D018836:Inflammation Mediators / Q000378:metabolism; D008099:Liver; D016031:Liver Transplantation; D008297:Male; D019188:Organ Preservation Solutions; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D024502:alpha-Tocopherol / Q000008:administration & dosage*
chemical_list: D016207:Cytokines; D017931:DNA Primers; D018836:Inflammation Mediators; D019188:Organ Preservation Solutions; D012333:RNA, Messenger; D045304:Cytochromes c; D000410:Alanine Transaminase; D024502:alpha-Tocopherol