title: Molecular detection of adenoviruses, rhabdoviruses, and paramyxoviruses in bats from Kenya.
abstract: We screened 217 bats of at least 20 species from 17 locations in Kenya during July and August of 2006 for the presence of adenovirus, rhabdovirus, and paramyxovirus nucleic acids using generic reverse transcription polymerase chain reaction (RT-PCR) and PCR assays. Of 217 bat fecal swabs examined, 4 bats were adenovirus DNA-positive, 11 bats were paramyxovirus RNA-positive, and 2 bats were rhabdovirus RNA-positive. Three bats were coinfected by two different viruses. By sequence comparison and phylogenetic analysis, the Kenya bat paramyxoviruses and rhabdoviruses from this study may represent novel viral lineages within their respective families; the Kenya bat adenoviruses could not be confirmed as novel, because the same region sequences from other known bat adenovirus genomes for comparison were lacking. Our study adds to previous evidence that bats carry diverse, potentially zoonotic viruses and may be coinfected with more than one virus.
authors.forename: Christina
authors.initials: C
authors.lastname: Conrardy
authors.forename: Ying
authors.initials: Y
authors.lastname: Tao
authors.forename: Ivan V
authors.initials: IV
authors.lastname: Kuzmin
authors.forename: Michael
authors.initials: M
authors.lastname: Niezgoda
authors.forename: Bernard
authors.initials: B
authors.lastname: Agwanda
authors.forename: Robert F
authors.initials: RF
authors.lastname: Breiman
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Anderson
authors.forename: Charles E
authors.initials: CE
authors.lastname: Rupprecht
authors.forename: Suxiang
authors.initials: S
authors.lastname: Tong
mesh_terms: D000256:Adenoviridae / Q000145:classification* / Q000235:genetics; D000257:Adenoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D002685:Chiroptera / Q000821:virology*; D060085:Coinfection; D005243:Feces / Q000821:virology; D005838:Genotype; D007630:Kenya / Q000453:epidemiology; D018184:Paramyxoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D018098:Paramyxovirinae / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D058974:Phylogeography; D015995:Prevalence; D012367:RNA, Viral / Q000145:classification* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012209:Rhabdoviridae / Q000145:classification* / Q000235:genetics; D018353:Rhabdoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology
chemical_list: D012367:RNA, Viral