title: Enzymatic etching of gold nanorods by horseradish peroxidase and application to blood glucose detection.
abstract: Gold nanorods (AuNRs) have become some of the most used nanostructures for biosensing and imaging applications due to their plasmon-related optical response, which is highly sensitive toward minute changes in the AuNR aspect ratio. In this context, H2O2 has been used to trigger the chemical etching of AuNRs, thereby inducing a decrease of their aspect ratio. However, special conditions and relatively high concentrations of H2O2 are usually required, preventing the applicability of the system for biodetection purposes. To overcome this limitation we have introduced a biocatalytic species, the enzyme horseradish peroxidase (HRP) that is able to induce a gradual oxidation of AuNRs in the presence of trace concentrations of H2O2. Interestingly, the presence of halide ions has also been found to be essential for this process. As a consequence, other enzymatic reactions, such as those catalyzed by glucose oxidase, can be easily coupled to HRP activity, allowing the detection of different amounts of glucose. On the basis of these findings, we developed a highly sensitive and simple colorimetric assay that can be read out by the naked eye and allows the detection of physiological glucose concentrations in human serum.
authors.affiliation: CIC biomaGUNE, Paseo de Miramón 182, 20009 Donostia/San Sebastián, Spain. vpavlov@cicbiomagune.es llizmarzan@cicbiomagune.es.
authors.forename: Laura
authors.initials: L
authors.lastname: Saa
authors.forename: Marc
authors.initials: M
authors.lastname: Coronado-Puchau
authors.forename: Valeri
authors.initials: V
authors.lastname: Pavlov
authors.forename: Luis M
authors.initials: LM
authors.lastname: Liz-Marzán
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D003124:Colorimetry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006219:Halogens / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D043942:Nanotubes / Q000737:chemistry*; D010084:Oxidation-Reduction; D058428:Silica Gel / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D006219:Halogens; D058428:Silica Gel; D006046:Gold; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase