title: PEX14 binding to Arabidopsis PEX5 has differential effects on PTS1 and PTS2 cargo occupancy of the receptor.
abstract: PEX5 acts as a cycling receptor for import of PTS1 proteins into peroxisomes and as a co-receptor for PEX7, the PTS2 receptor, but the mechanism of cargo unloading has remained obscure. Using recombinant protein domains we show PEX5 binding to the PEX14N-terminal domain (PEX14N) has no effect on the affinity of PEX5 for a PTS1 containing peptide. PEX5 can form a complex containing both recombinant PTS1 cargo and endogenous PEX7-thiolase simultaneously but isolation of the complex via the PEX14 construct resulted in an absence of thiolase, suggesting a possible role for PEX14 in the unloading of PTS2 cargos.
authors.affiliation: Centre for Plant Sciences, Irene Manton Building, University of Leeds, Leeds LS2 9JT, UK; School of Chemistry, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lanyon-Hogg
authors.affiliation: School of Chemistry, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: Jacob
authors.initials: J
authors.lastname: Hooper
authors.affiliation: Centre for Plant Sciences, Irene Manton Building, University of Leeds, Leeds LS2 9JT, UK; School of Chemistry, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: Sarah
authors.initials: S
authors.lastname: Gunn
authors.affiliation: School of Chemistry, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: Stuart L
authors.initials: SL
authors.lastname: Warriner
authors.affiliation: Centre for Plant Sciences, Irene Manton Building, University of Leeds, Leeds LS2 9JT, UK. Electronic address: a.baker@leeds.ac.uk.
authors.forename: Alison
authors.initials: A
authors.lastname: Baker
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D011485:Protein Binding; D021381:Protein Transport; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins; C499192:PEX14 protein, Arabidopsis; C000590561:PEX5 protein, Arabidopsis; C000614267:PEX7 protein, Arabidopsis; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D018160:Receptors, Cytoplasmic and Nuclear; D012097:Repressor Proteins
keywords: Arabidopsis thaliana; Cargo unloading; PEX14; PEX5; PEX7; PTS1; PTS2; Peroxisome