title: DNA and aptamer stabilized gold nanoparticles for targeted delivery of anticancer therapeutics.
abstract: Gold nanoparticles (GNPs) can be used as carriers of a variety of therapeutics. Ideally, drugs are released in the target cells in response to cell specific intracellular triggers. In this study, GNPs are loaded with doxorubicin or AZD8055, using a self-immolative linker which facilitates the release of anticancer therapeutics in malignant cells without modifications of the active compound. An additional modification with the aptamer AS1411 further increases the selectivity of GNPs towards cancer cells. Both modifications increase targeted delivery of therapeutics with GNPs. Whereas GNPs without anticancer drugs do not affect cell viability in all cells tested, AS1411 modified GNPs loaded with doxorubicin or AZD8055 show significant and increased reduction of cell viability in breast cancer and uveal melanoma cell lines. These results highlight that modified GNPs can be functionalized to increase the efficacy of cancer therapeutics and may further reduce toxicity by increasing targeted delivery towards malignant cells.
authors.affiliation: Instituto Madrileño de Estudios Avanzados en Nanociencia, CNB-CSIC-IMDEA Nanociencia Associated Unit, Cantoblanco, Madrid, Spain. alvaro.somoza@imdea.org.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Latorre
authors.forename: Christian
authors.initials: C
authors.lastname: Posch
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Garcimartín
authors.forename: Anna
authors.initials: A
authors.lastname: Celli
authors.forename: Martina
authors.initials: M
authors.lastname: Sanlorenzo
authors.forename: Igor
authors.initials: I
authors.lastname: Vujic
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Ma
authors.forename: Mitchell
authors.initials: M
authors.lastname: Zekhtser
authors.forename: Klemens
authors.initials: K
authors.lastname: Rappersberger
authors.forename: Susana
authors.initials: S
authors.lastname: Ortiz-Urda
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Somoza
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity; D052157:Aptamers, Nucleotide; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D018613:Microscopy, Confocal; D009025:Morpholines / Q000737:chemistry / Q000633:toxicity; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
chemical_list: C513936:AGRO 100; D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide; D004337:Drug Carriers; D009025:Morpholines; D009838:Oligodeoxyribonucleotides; D006046:Gold; D004317:Doxorubicin; D004247:DNA; C546624:(5-(2,4-bis((3S)-3-methylmorpholin-4-yl)pyrido(2,3-d)pyrimidin-7-yl)-2-methoxyphenyl)methanol