title: Helical DNA origami tubular structures with various sizes and arrangements.
abstract: We developed a novel method to design various helical tubular structures using the DNA origami method. The size-controlled tubular structures which have 192, 256, and 320 base pairs for one turn of the tube were designed and prepared. We observed the formation of the expected short tubes and unexpected long ones. Detailed analyses of the surface patterns of the tubes showed that the short tubes had mainly a left-handed helical structure. The long tubes mainly formed a right-handed helical structure and extended to the directions of the double helical axes as structural isomers of the short tubes. The folding pathways of the tubes were estimated by analyzing the proportions of short and long tubes obtained at different annealing conditions. Depending on the number of base pairs involved in one turn of the tube, the population of left-/right-handed and short/long tubes changed. The bending stress caused by the stiffness of the bundled double helices and the non-natural helical pitch determine the structural variety of the tubes.
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan). endo@kuchem.kyoto-u.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Endo
authors.forename: Seigi
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Tomoko
authors.initials: T
authors.lastname: Emura
authors.forename: Kumi
authors.initials: K
authors.lastname: Hidaka
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Morone
authors.forename: John E
authors.initials: JE
authors.lastname: Heuser
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA
keywords: DNA nanotechnology; DNA origami; DNA tube; atomic force microscopy; isomerization