title: Fenugreek seed extract inhibit fat accumulation and ameliorates dyslipidemia in high fat diet-induced obese rats.
abstract: This study investigated the inhibitory effect of aqueous extract of Trigonella foenum-graecum seeds (AqE-TFG) on fat accumulation and dyslipidemia in high fat diet- (HFD-) induced obese rats. Female Wistar rats were fed with HFD ad libitum, and the rats on HFD were treated orally with AqE-TFG or orlistat ((HFD for 28 days+AqE-TFG (0.5 and 1.0 g/kg) or orlistat (10 mg/kg) from day 8 to 28), respectively. Treatment with AqE-TFG produced significant reduction in body weight gain, body mass index (BMI), white adipose tissue (WAT) weights, blood glucose, serum insulin, lipids, leptin, lipase, and apolipoprotein-B levels and elevation in adiponectin levels. AqE-TFG improved serum aspartate amino transferase (AST), alanine amino transferase (ALT), and lactate dehydrogenase (LDH) levels. AqE-TFG treatment reduced the hepatic and cardiac thiobarbituric acid reactive substances (TBARS) and elevated the antioxidant enzyme (glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT)) levels. In addition, liver and uterine WAT lipogenic enzyme (fatty acid synthetase (FAS) and glucose-6-phosphate dehydrogenase (G6PD)) activities were restored towards normal levels. These findings demonstrated the preventive effect of AqE-TFG on fat accumulation and dyslipidemia, due to inhibition of impaired lipid digestion and absorption, in addition to improvement in glucose and lipid metabolism, enhancement of insulin sensitivity, increased antioxidant defense, and downregulation of lipogenic enzymes.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Hamdard University, New Delhi 110062, India.
authors.forename: Parveen
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Hamdard University, New Delhi 110062, India.
authors.forename: Uma
authors.initials: U
authors.lastname: Bhandari
authors.affiliation: Department of Pharmacology, National Research Institute of Ayurvedic Drug Development, Kolkata 700091, India.
authors.forename: Shrirang
authors.initials: S
authors.lastname: Jamadagni
mesh_terms: D052436:Adipose Tissue, White; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D000886:Anthropometry; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D015992:Body Mass Index; D001835:Body Weight; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D050171:Dyslipidemias / Q000188:drug therapy*; D005260:Female; D006706:Homeostasis; D007783:Lactones / Q000627:therapeutic use; D009765:Obesity; D000077403:Orlistat; D018384:Oxidative Stress; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D029910:Trigonella / Q000737:chemistry*; D015430:Weight Gain
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D007783:Lactones; D010936:Plant Extracts; D000077403:Orlistat