title: Zeptomole detection of C-reactive protein in serum by a nanoparticle amplified surface plasmon resonance imaging aptasensor.
abstract: Diagnostic biomarkers (i.e. proteins) are often in low abundance in bodily fluids presenting many challenges for their detection. In order to extend the application of SPRi systems in detecting biomarkers at ultralow levels, we combine the advantage of aptamer technology with nanomaterials and microwave-assisted surface functionalization. By implementing a sandwich assay through the introduction of aptamer-modified quantum dots (QDs), it was possible to measure 7 zeptomole (at 5 fg/mL) of C-reactive protein (CRP) selectively in spiked human serum. It is expected that the proposed platform will provide new direction in designing ultrasensitive SPRi biosensors with multiplexing capabilities.
authors.affiliation: Department of Nanoscience, University of North Carolina at Greensboro, 2907 E. Lee Street, Greensboro, NC, USA 27401.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Vance
authors.affiliation: Department of Nanoscience, University of North Carolina at Greensboro, 2907 E. Lee Street, Greensboro, NC, USA 27401.
authors.forename: Marinella G
authors.initials: MG
authors.lastname: Sandros
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D015374:Biosensing Techniques / Q000295:instrumentation*; D001774:Blood Chemical Analysis / Q000295:instrumentation; D002097:C-Reactive Protein / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008832:Microchemistry / Q000295:instrumentation; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold; D002097:C-Reactive Protein