title: Evaluation of immune and apoptosis related gene responses using an RNAi approach in vaccinated Penaeus monodon during oral WSSV infection.
abstract: In the present study RNA interference was used to elucidate the connection between two endogenous genes [Penaeus monodon Rab7 (PmRab7) or P. monodon inhibitor of apoptosis (PmIAP)], and selected immune/apoptosis-related genes in orally 'vaccinated' shrimp after white spot syndrome virus (WSSV) infection. P. monodon were vaccinated by feeding them with formalin inactivated WSSV-coated feed. Thereafter, PmRab7 or PmIAP genes were silenced by injecting the shrimps with their respective dsRNA. The resulting groups of shrimps, Rab7 and IAP, were orally infected with WSSV and the expression of three immune-relevant genes in Rab7 group and five apoptosis-related genes in IAP group was evaluated. In the Rab7 group, PmToll, PmPPAE 2 and Pm penaeidin genes were down-regulated. The IAP-silenced shrimps were characterized by down-regulation of Pm caspase, PmERp57, Pm14-3-3 ε, Pm ald, and up-regulation of PmSTAT. Thus, silencing of PmRab7/PmIAP has provided important clues on their relationship with selected immune/apoptosis genes in orally vaccinated P. monodon during WSSV infection.
authors.affiliation: Faculty of Biosciences and Aquaculture, University of Nordland, 8049, Bodø, Norway.
authors.forename: Amod D
authors.initials: AD
authors.lastname: Kulkarni
authors.affiliation: Faculty of Biosciences and Aquaculture, University of Nordland, 8049, Bodø, Norway.
authors.forename: Christopher M A
authors.initials: CM
authors.lastname: Caipang
authors.affiliation: Faculty of Biosciences and Aquaculture, University of Nordland, 8049, Bodø, Norway. Electronic address: kvi@uin.no.
authors.forename: Viswanath
authors.initials: V
authors.lastname: Kiron
authors.affiliation: Faculty of Biosciences and Aquaculture, University of Nordland, 8049, Bodø, Norway; Wageningen University, Wageningen, The Netherlands.
authors.forename: Jan H W M
authors.initials: JH
authors.lastname: Rombout
authors.affiliation: Faculty of Biosciences and Aquaculture, University of Nordland, 8049, Bodø, Norway.
authors.forename: Jorge M O
authors.initials: JM
authors.lastname: Fernandes
authors.affiliation: Faculty of Biosciences and Aquaculture, University of Nordland, 8049, Bodø, Norway.
authors.forename: Monica F
authors.initials: MF
authors.lastname: Brinchmann
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D005786:Gene Expression Regulation / Q000276:immunology*; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics; D033561:Penaeidae / Q000235:genetics / Q000276:immunology / Q000821:virology; D034622:RNA Interference; D014765:Viral Vaccines / Q000276:immunology; D046848:White spot syndrome virus 1 / Q000502:physiology*; D020691:rab GTP-Binding Proteins / Q000235:genetics; D000090802:rab7 GTP-Binding Proteins
chemical_list: D051034:Inhibitor of Apoptosis Proteins; D014765:Viral Vaccines; D000090802:rab7 GTP-Binding Proteins; D020691:rab GTP-Binding Proteins
keywords: Apoptosis; Gene silencing; Immune; Penaeus monodon; WSSV