title: Cellular imaging of deep organ using two-photon Bessel light-sheet nonlinear structured illumination microscopy.
abstract: In vivo fluorescent cellular imaging of deep internal organs is highly challenging, because the excitation needs to penetrate through strong scattering tissue and the emission signal is degraded significantly by photon diffusion induced by tissue-scattering. We report that by combining two-photon Bessel light-sheet microscopy with nonlinear structured illumination microscopy (SIM), live samples up to 600 microns wide can be imaged by light-sheet microscopy with 500 microns penetration depth, and diffused background in deep tissue light-sheet imaging can be reduced to obtain clear images at cellular resolution in depth beyond 200 microns. We demonstrate in vivo two-color imaging of pronephric glomeruli and vasculature of zebrafish kidney, whose cellular structures located at the center of the fish body are revealed in high clarity by two-color two-photon Bessel light-sheet SIM.
authors.affiliation: College of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA.
authors.forename: Han
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA.
authors.forename: Amit
authors.initials: A
authors.lastname: Ashok
authors.affiliation: College of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA.
authors.forename: Rongguang
authors.initials: R
authors.lastname: Liang
authors.affiliation: Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, MI 48109, USA.
authors.forename: Weibin
authors.initials: W
authors.lastname: Zhou
authors.affiliation: College of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA.
authors.forename: Leilei
authors.initials: L
authors.lastname: Peng
keywords: (170.2520) Fluorescence microscopy; (170.6900) Three-dimensional microscopy