title: Characterization of a versatile arylesterase from Lactobacillus plantarum active on wine esters.
abstract: The gene lp_1002 from Lactobacillus plantarum WCFS1 encoding a putative lipase/esterase was cloned and overexpressed in Escherichia coli BL21(DE3). The purified Lp_1002 protein was biochemically characterized. Lp_1002 is an arylesterase which showed high hydrolytic activity on phenyl acetate. Although to a lesser extent, Lp_1002 also hydrolyzed most of the esters assayed including relevant wine aroma compounds. Importantly, Lp_1002 exhibited hydrolytic activity at winemaking conditions, although optimal catalytic activity is observed at 40 °C and pH 5-7. The effect of wine compounds on Lp_1002 activity was assayed. From the compounds assayed (ethanol, sodium metabisulfite, and malic, tartaric, lactic and citric acids), only malic acid slightly inhibited Lp_1002 activity. Lp_1002 is the first arylesterase described in a wine lactic acid bacteria and possessed suitable biochemical properties to be used during winemaking.
authors.affiliation: Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de Alimentos y Nutrición, ICTAN-CSIC , Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Esteban-Torres
authors.forename: José María
authors.initials: JM
authors.lastname: Barcenilla
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Mancheño
authors.forename: Blanca
authors.initials: B
authors.lastname: de las Rivas
authors.forename: Rosario
authors.initials: R
authors.lastname: Muñoz
mesh_terms: D000085:Acetates / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055162:Biocatalysis; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004795:Enzyme Stability; D004952:Esters / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010636:Phenols / Q000378:metabolism; D016415:Sequence Alignment; D013379:Substrate Specificity; D014920:Wine / Q000032:analysis / Q000382:microbiology*
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D004952:Esters; D010636:Phenols; C570634:phenyl acetate; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase