title: Synthetic polyamine BPA-C8 inhibits TGF-β1-mediated conversion of human dermal fibroblast to myofibroblasts and establishment of galectin-1-rich extracellular matrix in vitro.
abstract: Cancer-associated fibroblasts (CAFs) play a role in the progression of malignant tumors. They are formed by conversion of fibroblasts to smooth muscle α-actin-positive (SMA-positive) myofibroblasts. Polyamines are known to change the arrangement of the actin cytoskeleton by binding to the anionic actin. We tested the effect of the synthetic polyamine BPA-C8 on the transition of human dermal fibroblasts to myofibroblasts induced either by TGF-β1 alone or by TGF-β1 together with adhesion/growth-regulatory galectin-1. Pre-existing CAFs, myofibroblasts from pancreatitis, and rat smooth muscle cells were also exposed to BPA-C8. BPA-C8 impaired myofibroblast formation from activated fibroblasts, but it had no effect on cells already expressing SMA. BPA-C8 also reduced the occurrence of an extracellular matrix around the activated fibroblasts. The reported data thus extend current insights into polyamine activity, adding interference with tumor progression to the tumor-promoting processes warranting study.
authors.affiliation: Institute of Anatomy, Charles University, 1st Faculty of Medicine, U Nemocnice 3, 128 00 Prague (Czech Republic); Department of Otorhinolaryngology and Head and Neck Surgery, Charles University, 1st Faculty of Medicine, V Úvalu 5, 150 00 Prague (Czech Republic).
authors.forename: Alžběta
authors.initials: A
authors.lastname: Mifková
authors.forename: Ondřej
authors.initials: O
authors.lastname: Kodet
authors.forename: Pavol
authors.initials: P
authors.lastname: Szabo
authors.forename: Jan
authors.initials: J
authors.lastname: Kučera
authors.forename: Barbora
authors.initials: B
authors.lastname: Dvořánková
authors.forename: Sabine
authors.initials: S
authors.lastname: André
authors.forename: Girish
authors.initials: G
authors.lastname: Koripelly
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Gabius
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Lehn
authors.forename: Karel
authors.initials: K
authors.lastname: Smetana
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology*; D037483:Galectin 1 / Q000378:metabolism*; D006801:Humans; D058628:Myofibroblasts / Q000187:drug effects* / Q000473:pathology*; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D011073:Polyamines / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000199:Actins; D037483:Galectin 1; D011073:Polyamines; D053773:Transforming Growth Factor beta1
keywords: actin; cancer; galectin; polycations; tenascin