title: Mass spectrometric detection of nanoparticle host-guest interactions in cells.
abstract: Synthetic host-guest chemistry is a versatile tool for biomedical applications. Characterization and detection of host-guest complexes in biological systems, however, is challenging due to the complexity of the biological milieu. Here, we describe and apply a mass spectrometric method to monitor the association and dissociation of nanoparticle (NP)-based host-guest interactions that integrates NP-assisted laser desorption/ionization (LDI) and matrix assisted laser desoption/ionization (MALDI) mass spectrometry. This LDI/MALDI approach reveals how NP surface functionality affects host-guest interactions in cells, information difficult to achieve using other techniques.
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , Amherst, Massachusetts 01003, United States.
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
authors.forename: Gulen
authors.initials: G
authors.lastname: Yesilbag Tonga
authors.forename: Singyuk
authors.initials: S
authors.lastname: Hou
authors.forename: Patrick W
authors.initials: PW
authors.lastname: Fedick
authors.forename: Yi-Cheun
authors.initials: YC
authors.lastname: Yeh
authors.forename: Felix S
authors.initials: FS
authors.lastname: Alfonso
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Mizuhara
authors.forename: Richard W
authors.initials: RW
authors.lastname: Vachet
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
mesh_terms: D003593:Cytoplasm / Q000378:metabolism*; D006046:Gold / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D047028:Macrocyclic Compounds / Q000737:chemistry / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013499:Surface Properties; D000099246:Cucurbit(n)urils
chemical_list: D006046:Gold; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils