title: Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering.
abstract: Electrospun hybrid nanofibers prepared using combinations of natural and synthetic polymers have been widely investigated in cardiovascular tissue engineering. In this study, electrospun gelatin/polycaprolactone (PCL) and collagen/poly(l-lactic acid-co-ε-caprolactone) (PLCL) scaffolds were successfully produced. Scanning electron micrographs showed that fibers of both membranes were smooth and homogeneous. Water contact angle measurements further demonstrated that both scaffolds were hydrophilic. To determine cell attachment and migration on the scaffolds, both hybrid scaffolds were seeded with human umbilical arterial smooth muscle cells. Scanning electron micrographs and MTT assays showed that the cells grew and proliferated well on both hybrid scaffolds. Gross observation of the transplanted scaffolds revealed that the engineered collagen/PLCL scaffolds were smoother and brighter than the gelatin/PCL scaffolds. Hematoxylin and eosin staining showed that the engineered blood vessels constructed by collagen/PLCL electrospun membranes formed relatively homogenous vessel-like tissues. Interestingly, Young's modulus for the engineered collagen/PLCL scaffolds was greater than for the gelatin/PCL scaffolds. Together, these results indicate that nanofibrous collagen/PLCL membranes with favorable mechanical and biological properties may be a desirable scaffold for vascular tissue engineering.
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China ; Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fu
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Zhenling
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China ; Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Bei
authors.initials: B
authors.lastname: Feng
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Renjie
authors.initials: R
authors.lastname: Hu
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yin
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology / Q000254:growth & development*; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D019245:Compressive Strength; D055119:Elastic Modulus; D004595:Electroplating / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005780:Gelatin / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry*; D013718:Tensile Strength; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; C505727:poly(epsilon-caprolactone-co-lactide); C016240:polycaprolactone; D005780:Gelatin; D003094:Collagen
keywords: collagen; electrospinning; gelatin; poly(l-lactic acid-co-ε-caprolactone); polycaprolactone