title: Flavihumibacter solisilvae sp. nov., isolated from forest soil.
abstract: A Gram-stain-positive, strictly aerobic, yellow colony-forming bacterium, designated strain 3-3(T), was isolated from forest soil of Bac Kan Province in Vietnam. Cells were non-motile rods without gliding motility, showing oxidase- and catalase-positive reactions. Growth was observed at 20-37 °C (optimum, 28 °C) and pH 5.5-9.5 (optimum, pH 7.5). The major cellular fatty acids were iso-C(15 : 0), iso-C(15 : 1) G and summed feature 3 (comprising C(16 : 1)ω6c and/or C(16 : 1)ω7c). Strain 3-3(T) contained phosphatidylethanoamine, three unidentified aminolipids and three unidentified lipids. The G+C content of the genomic DNA was 49.5 mol% and the only isoprenoid quinone detected was menaquinone 7 (MK-7). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-3(T) formed a tight phylogenetic lineage with Flavihumibacter petaseus T41(T) with a bootstrap value of 100%. Strain 3-3(T) was related most closely to F. petaseus T41(T) with 97.3% 16S rRNA gene sequence similarity and the level of DNA-DNA relatedness between the two was 9.4±1.2%. Based on phenotypic, chemotaxonomic and molecular features, strain 3-3(T) represents a novel species of the genus Flavihumibacter, for which the name Flavihumibacter solisilvae sp. nov. is proposed. The type strain is 3-3(T) ( = KACC 17917(T) = JCM 19891(T)).
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Sang Eun
authors.initials: SE
authors.lastname: Jeong
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon, 440-746, Republic of Korea.
authors.forename: Myong-Suk
authors.initials: MS
authors.lastname: Cho
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon, 440-746, Republic of Korea.
authors.forename: SeonHee
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Animal Science & Technology, Sunchon National University, Sunchon 540-742, Republic of Korea.
authors.forename: Sang-Suk
authors.initials: SS
authors.lastname: Lee
authors.affiliation: National Institute of Biological Resources, Hwangyeong-ro 42, Seo-gu, Incheon, 404-708, Republic of Korea.
authors.forename: Byoung-Hee
authors.initials: BH
authors.lastname: Lee
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D011090:Polyenes / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees; D014744:Vietnam; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D011090:Polyenes; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C017313:flexirubins; C062629:menaquinone 7