title: A new approach to ELISA-based anti-glycolipid antibody evaluation of highly adhesive serum samples.
abstract: The enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay used in measuring antibody reactivity (expressed as titers) for glycosphingolipids (GSLs) such as gangliosides and sulfoglycolipids in the sera of patients with Guillain-Barré syndrome (GBS), variants of GBS, and chronic inflammatory demyelinating polyneuropathy (CIDP). In the present study, anti-GSL antibodies were evaluated using a new formula of affinity parametric complex (APC), calculated from limiting-dilution serum assay data, followed by affinity parametric complex criterion (APCC). Using assay results based on APCC, we analyzed serum samples categorized into acute inflammatory demyelinating polyneuropathy (AIDP), acute motor-sensory axonal neuropathy (AMSAN), CIDP, CIDP with myasthenia gravis (MG), and amyotrophic lateral sclerosis (ALS). We were able to determine the affinity strength of antibodies otherwise hidden in the non-specific background activity in highly adhesive serum samples. The thin-layer chromatography (TLC)-immuno-overlay method assured us that this new method is an accurate and reliable way for evaluating anti-GSL antibodies using ELISA serum sample data.
authors.affiliation: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Seigo
authors.initials: S
authors.lastname: Usuki
authors.affiliation: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Dawn
authors.initials: D
authors.lastname: O'Brien
authors.affiliation: Department of Neurology, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Rivner
authors.affiliation: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA; Department of Neurology, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA. Electronic address: RYU@GRU.edu.
authors.forename: Robert K
authors.initials: RK
authors.lastname: Yu
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002855:Chromatography, Thin Layer; D003198:Computer Simulation; D004797:Enzyme-Linked Immunosorbent Assay; D006028:Glycosphingolipids / Q000276:immunology*; D020275:Guillain-Barre Syndrome / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D015417:Hereditary Sensory and Motor Neuropathy / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D015233:Models, Statistical; D020277:Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D011237:Predictive Value of Tests; D011485:Protein Binding; D015203:Reproducibility of Results
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D006028:Glycosphingolipids
keywords: ALS; Anti-glycolipid antibody; CIDP; ELISA; GBS; Non-specific adhesions