title: Identification of the bioactive components of orally administered Lithocarpus polystachyus Rehd and their metabolites in rats by liquid chromatography coupled to LTQ Orbitrap mass spectrometry.
abstract: A rapid, sensitive and selective UPLC/MS method using LTQ Orbitrap mass spectrometry was established for the analysis and characterization of the main biological components and their metabolites in rat plasma, urine and feces following oral administration of Lithocarpus polystachyus extract. In vivo, 22 flavonoid metabolites were observed in rat plasma, and 13 metabolites were detected in rat urine, whereas just two aglycones of dihydrochalcone (3-hydroxy phloretin and phloretin) could be detected in rat feces. Among these metabolites, one new and a known dihydrochalcone metabolite were isolated and definitely identified. Besides, five dihydrochalcone metabolites were tentatively identified as new compounds. A metabolism study of 3-hydroxyphlorizin and phloridzin was also conducted. Glucuronidation was the main metabolic pathway of phloridzin, whereas glucuronidation and sulfonation were the main metabolic pathway of 3-hydroxyphlorizin. These results provided a basis for evaluating the bioactive components of a complex natural medicine and their mechanisms of actions.
authors.affiliation: Second Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Chinese Pharmaceutical Science, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Chinese Pharmaceutical Science, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China.
authors.forename: Shaozhen
authors.initials: S
authors.lastname: Hou
authors.affiliation: School of Chinese Pharmaceutical Science, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China.
authors.forename: Song
authors.initials: S
authors.lastname: Huang
authors.affiliation: School of Chinese Pharmaceutical Science, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China.
authors.forename: Weiqin
authors.initials: W
authors.lastname: Yang
authors.affiliation: School of Chinese Pharmaceutical Science, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China. Electronic address: youren1994@aliyun.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Lai
authors.affiliation: Second Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China. Electronic address: zengxing-china@163.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D029962:Fagaceae / Q000737:chemistry*; D005243:Feces / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D010936:Plant Extracts / Q000032:analysis / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D005419:Flavonoids; D010936:Plant Extracts
keywords: Biological components; Lithocarpus polystachyus; Metabolites; UPLC–MS/MS