title: KAP-1 is overexpressed and correlates with increased metastatic ability and tumorigenicity in pancreatic cancer.
abstract: This study aimed to investigate the role in metastasis and prognostic value of KAP-1 in pancreatic cancer (PC). The expression of KAP-1 was analyzed by quantitative real-time polymerase chain reaction, Western blotting, and immunohistochemical staining in 91 human PC tissue samples. Capan-2 cells were transfected with a lentiviral vector expressing KAP-1 (Capan-2/KAP-1) or the empty vector (Capan-2/vector); cell migration and invasion were assayed in vitro using Transwell migration and wound-healing assays, and in vivo using a xenograft model in nude mice. KAP-1 was found to be overexpressed in human PC, and the expression of KAP-1 correlated with clinical stage. Overexpression of KAP-1 increased the invasion and migration of Capan-2 cells in vitro. Furthermore, overexpression of KAP-1 promoted the growth and metastatic ability of PC cells in a xenograft model in nude mice. Moreover, overexpression of KAP-1 induced the epithelial-mesenchymal transition (EMT) in PC cells both in vitro and in vivo, as indicated by increased expression of mesenchymal markers such as vimentin and decreased expression of E-cadherin. This study indicates that KAP-1 may promote metastasis in PC by regulating the EMT and suggests that KAP-1 may have potential as a predictor of metastasis in patients with pancreatic cancer.
authors.affiliation: Department of Biliary-Hepatic Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Street, Guiyang, 550001, Guizhou, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhan
authors.forename: Jianxin
authors.initials: J
authors.lastname: Jiang
authors.forename: Yaozhen
authors.initials: Y
authors.lastname: Pan
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Pen
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Renyi
authors.initials: R
authors.lastname: Qin
authors.forename: Chenyi
authors.initials: C
authors.lastname: Sun
mesh_terms: D000368:Aged; D000818:Animals; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008819:Mice, Nude; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D000076183:Tripartite Motif-Containing Protein 28; D023041:Xenograft Model Antitumor Assays
chemical_list: D012097:Repressor Proteins; C101116:TRIM28 protein, human; D000076183:Tripartite Motif-Containing Protein 28