title: Acute radiation enteritis caused by dose-dependent radiation exposure in dogs: experimental research.
abstract: Accidental or intended radiation exposure in mass casualty settings presents a serious and on-going threat. The development of mitigating and treating agents requires appropriate animal models. Unfortunately, the majority of research on radiation enteritis in animals has lacked specific assessments and targeted therapy. Our study showed beagle dogs, treated by intensity-modulated radiation therapy (IMRT) for abdominal irradiation, were administered single X-ray doses of 8-30 Gy. The degree of intestinal tract injury for all of the animals after radiation exposure was evaluated with regard to clinical syndrome, endoscopic findings, histological features, and intestinal function. The range of single doses (8 Gy, 10-14 Gy, and 16-30 Gy) represented the degree of injury (mild, moderate, and severe, respectively). Acute radiation enteritis included clinical syndrome with fever, vomiting, diarrhea, hemafecia, and weight loss; typical endoscopic findings included edema, bleeding, mucosal abrasions, and ulcers; and intestinal biopsy results revealed mucosal necrosis, erosion, and loss, inflammatory cell infiltration, hemorrhage, and congestion. Changes in serum diamine oxides (DAOs) and d-xylose represented intestinal barrier function and absorption function, respectively, and correlated with the extent of damage (P < 0.05 and P < 0.05, respectively). We successfully developed a dog model of acute radiation enteritis, thus obtaining a relatively objective evaluation of intestinal tract injury based on clinical performance and laboratory examination. The method of assessment of the degree of intestinal tract injury after abdominal irradiation could be beneficial in the development of novel and effective therapeutic strategies for acute radiation enteritis.
authors.affiliation: Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wenda
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, Shenyang General Hospital of PLA, Shenyang 110840, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, Shenyang General Hospital of PLA, Shenyang 110840, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, Shenyang General Hospital of PLA, Shenyang 110840, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Shenyang General Hospital of PLA, Shenyang 110840, China guoxiaozhong1958@163.com.
authors.forename: Xiaozhong
authors.initials: X
authors.lastname: Guo
mesh_terms: D000818:Animals; D004285:Dogs; D004751:Enteritis / Q000473:pathology*; D007422:Intestines / Q000473:pathology* / Q000503:physiopathology*; D011833:Radiation Injuries, Experimental / Q000473:pathology*
keywords:     d-xylose; Acute radiation enteritis; diamine oxides; dogs; endoscopy