title: Molecular typing of Legionella pneumophila serogroup 1 clinical strains isolated in Italy.
abstract: Molecular typing methods for discriminating different bacterial isolates are essential epidemiological tools in prevention and control of Legionella infections and outbreaks. A selection of 56 out of 184 Legionella pneumophila serogroup 1 (Lp1) clinical isolates, collected from different Italian regions between 1987 and 2012, and stored at the National Reference Laboratory for Legionella, were typed by monoclonal antibody (MAb) subgrouping, amplified fragment length polymorphism (AFLP) and sequence based typing (SBT). These strains were isolated from 39 community (69.6%), 14 nosocomial (25%) and 3 travel associated (5.4%) Legionnaires'disease cases. MAb typing results showed a prevalence of MAb 3/1 positive isolates (75%) with the Philadelphia subgroup representing 35.7%, followed by Knoxville (23.2%), Benidorm (12.5%), Allentown/France (1.8%), Allentown/France-Philadelphia (1.8%). The remaining 25% were MAb 3/1 negative, namely 11 Olda (19.6%), 2 Oxford (3.6%) and 1 Bellingham (1.8%) subgroups. AFLP analysis detected 20 different genomic profiles. SBT analysis revealed 32 different sequence types (STs) with high diversity of STs (IODSTs=0.952) 12 of which were never described before. ST1 and ST23 were most frequently isolated as observed worldwide. A helpful analysis of data from SBT, MAb subgrouping and AFLP is provided, as well as a comparison to the Lp1 types investigated from other countries. This study describes the first Italian Lp1 strains database, providing molecular epidemiology data useful for future epidemiological investigations, especially of travel associated Legionnaires' diseases (TALD) cases, Italy being the country associated with the highest number of clusters.
authors.affiliation: Istituto Superiore di Sanità (ISS, National Institute of Health), Department of Infectious Parasitic and Immune-mediate diseases, Rome, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Fontana
authors.affiliation: Istituto Superiore di Sanità (ISS, National Institute of Health), Department of Infectious Parasitic and Immune-mediate diseases, Rome, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Scaturro
authors.affiliation: Istituto Superiore di Sanità (ISS, National Institute of Health), National Centre for Epidemiology, Surveillance and Health Promotion, Rome, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Rota
authors.affiliation: Istituto Superiore di Sanità (ISS, National Institute of Health), National Centre for Epidemiology, Surveillance and Health Promotion, Rome, Italy.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Caporali
authors.affiliation: Istituto Superiore di Sanità (ISS, National Institute of Health), Department of Infectious Parasitic and Immune-mediate diseases, Rome, Italy. Electronic address: marialuisa.ricci@iss.i.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Ricci
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D005838:Genotype; D006801:Humans; D007558:Italy / Q000453:epidemiology; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D007877:Legionnaires' Disease / Q000453:epidemiology* / Q000382:microbiology*; D058889:Molecular Typing; D017422:Sequence Analysis, DNA; D065288:Serogroup; D012703:Serotyping
keywords: AFLP; Legionella; MAb subgroup; SBT; Typing