title: Amphiplicatus metriothermophilus gen. nov., sp. nov., a thermotolerant alphaproteobacterium isolated from a hot spring.
abstract: A thermotolerant, Gram-strain-negative, non-spore-forming and strictly aerobic bacterium, designated GU51(T), was isolated from Guhai hot spring in Jimsar county, Xinjiang province, north-west China. Each cell of strain GU51(T) consisted of an oval body and two symmetrical long (3-6 µm) prosthecae. The strain moved by polar flagellum. Oxidase and catalase were produced. Strain GU51(T) grew within the ranges of 37-65 °C (optimum 48-50 °C), 0.5-7.5% (w/v) NaCl (optimum 2-3%) and pH 6.0-9.0 (optimum pH 7.5). The major respiratory quinone detected was ubiquinone 10 (U-10) and the genomic DNA G+C content was 66.7±0.4 mol%. Major fatty acids (>5%) were C(16 : 0), C(18 : 1)ω7c and 11-methyl C(18 : 1)ω7c. The polar lipids consisted of diphosphatidylglycerol, five glycolipids, phosphatidylglycerol and an unknown phospholipid. Phylogenetic analysis showed the closest relatives of strain GU51(T) were members of the genus Parvularcula with 92.3% 16S rRNA gene sequence similarity. On the basis of this polyphasic taxonomic characterization, it is suggested that strain GU51(T) represents a novel species of a new genus in the family 'Parvularculaceae', for which the name Amphiplicatus metriothermophilus gen. nov., sp. nov. is proposed. The type strain of the type species is GU51(T) ( = CGMCC 1.12710(T) = JCM 19779(T)).
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zhen-Li
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Xin-Qi
authors.affiliation: Key Laboratory of Biogeography and Bioresources in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Xinjiang 830011, PR China.
authors.forename: Wu
authors.initials: W
authors.lastname: Nan
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Wen-Wu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Xu-Fen
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Cao
authors.initials: C
authors.lastname: Yi
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Wu
authors.initials: W
authors.lastname: Min
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2