title: Molecular identification of the agent of Q fever - Coxiella burnetii - in domestic animals in State of Rio de Janeiro, Brazil.
abstract: BACKGROUND
  Over the last recent years, the number of Q fever cases have has increased throughout the world. An epidemiological investigation was performed in the area in which the first molecular documentation of Q fever in Brazil was previously reported.
  
  
  METHODS
  Indirect immunofluorescence assay (IFA) and PCR of Coxiella burnetii targeting the htpAB gene were performed in samples from 14 dogs (blood); 1 cat (blood); 10 goats (blood, milk, vaginal swab and anal swab); 3 sheep (blood); and 2 horses (blood).
  
  
  RESULTS
  Two dogs, two sheep and five goats were seroreactive. DNA was amplified from 6 milk and 2 blood samples from goats and from dogs, respectively. The sequence of the amplicons exhibited 99% sequence similarity with the homologous sequence of the htpAB gene of C. burnetii RSA 331 (GenBank - CP000890).
  
  
  CONCLUSIONS
  The results confirm C. burnetii infection in animals in Rio de Janeiro and reinforce the need for the surveillance of Q fever in Brazil.
authors.affiliation: Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Maria Angélica Monteiro de Mello
authors.initials: MA
authors.lastname: Mares-Guia
authors.affiliation: Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Rozental
authors.affiliation: Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Alexandro
authors.initials: A
authors.lastname: Guterres
authors.affiliation: Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Raphael
authors.initials: R
authors.lastname: Gomes
authors.affiliation: Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Daniele Nunes de
authors.initials: DN
authors.lastname: Almeida
authors.affiliation: Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Namir Santos
authors.initials: NS
authors.lastname: Moreira
authors.affiliation: Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: Jairo Dias
authors.initials: JD
authors.lastname: Barreira
authors.affiliation: Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Alexsandra Rodrigues
authors.initials: AR
authors.lastname: Favacho
authors.affiliation: Secretaria de Saúde do Município de Itaboraí, Itaborarí, RJ.
authors.forename: Andrea Lopes
authors.initials: AL
authors.lastname: Santana
authors.affiliation: Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Elba Regina Sampaio de
authors.initials: ER
authors.lastname: Lemos
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D001938:Brazil; D002415:Cats; D016997:Coxiella burnetii / Q000235:genetics*; D004285:Dogs; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006041:Goats; D006736:Horses; D016133:Polymerase Chain Reaction; D011778:Q Fever / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D012756:Sheep