title: Detection of Schistosoma mansoni infection by TaqMan® Real-Time PCR in a hamster model.
abstract: An experimental study in hamsters was performed to evaluate the capability for detecting Schistosoma mansoni DNA in serum and fecal samples during the pre and post-egg-laying periods of infection using TaqMan® Real-Time PCR system (qPCR), was compared with the circumoval precipitin test (COPT) and the Kato-Katz technique, especially among individuals with low parasitic burden. Twenty-four hamsters were infected with cercariae. Three hamsters were sacrificed per week under anesthesia, from 7 days post infection (DPI) up to 56 DPI. A serum sample and a pool of feces were collected from each hamster. The presence of S. mansoni eggs in fecal samples was evaluated by Kato-Katz method and in the hamsters gutby histopathology. Detection of S. mansoni DNA was performed using qPCR and S. mansoni antibody using COPT. The first detection of eggs in feces by Kato-Katz method and S. mansoni DNA in feces by qPCR occurred 49 DPI. Nevertheless, S. mansoni DNA was detected in serum samples from 14 up to 56 DPI. COPT was positive at 35 DPI. The results not only confirm the reliability of S. mansoni DNA detection by qPCR, but also demonstrate that serum is a trustworthy source of DNA in the pre patent infection period.
authors.affiliation: Department of Infectious and Parasitic Diseases and Laboratory of Medical Investigation 6, Faculdade de Medicina da Universidade de São Paulo, Brazil. Electronic address: cristinasanto@usp.br.
authors.forename: Maria Cristina Carvalho
authors.initials: MC
authors.lastname: Espírito-Santo
authors.affiliation: Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: Mónica Viviana
authors.initials: MV
authors.lastname: Alvarado-Mora
authors.affiliation: Department of Enteroparasites of the Center of Parasitology and Mycology, Instituto Adolfo Lutz da Secretaria de Estado da Saúde de São Paulo, Brazil.
authors.forename: Pedro Luiz Silva
authors.initials: PL
authors.lastname: Pinto
authors.affiliation: Department of Infectious and Parasitic Diseases and Laboratory of Medical Investigation 6, Faculdade de Medicina da Universidade de São Paulo, Brazil; Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Brazil.
authors.forename: Thales
authors.initials: T
authors.lastname: de Brito
authors.affiliation: Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: Lívia
authors.initials: L
authors.lastname: Botelho-Lima
authors.affiliation: Sigma Life Sciences, Woodlands, TX, USA.
authors.forename: Ashley Richard
authors.initials: AR
authors.lastname: Heath
authors.affiliation: Laboratory of Medical Genomics, Center for International Research and Education (CIPE), Hospital AC Camargo, Brazil.
authors.forename: Maria Galli
authors.initials: MG
authors.lastname: Amorim
authors.affiliation: Laboratory of Medical Genomics, Center for International Research and Education (CIPE), Hospital AC Camargo, Brazil; Laboratory of Neurociencies (LIM-27), Department and Institute of Psychiatry, Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Dias-Neto
authors.affiliation: Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, Brazil; Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Brazil.
authors.forename: Pedro Paulo
authors.initials: PP
authors.lastname: Chieffi
authors.affiliation: Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: João Renato Rebello
authors.initials: JR
authors.lastname: Pinho
authors.affiliation: Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: Flair José
authors.initials: FJ
authors.lastname: Carrilho
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Brazil.
authors.forename: Expedito José Albuquerque
authors.initials: EJ
authors.lastname: Luna
authors.affiliation: Department of Infectious and Parasitic Diseases and Laboratory of Medical Investigation 6, Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: Ronaldo Cesar Borges
authors.initials: RC
authors.lastname: Gryschek
mesh_terms: D000818:Animals; D001700:Biomphalaria; D006224:Cricetinae; D018509:DNA, Helminth / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D004195:Disease Models, Animal; D005243:Feces / Q000469:parasitology; D007422:Intestines / Q000469:parasitology / Q000473:pathology; D007668:Kidney / Q000469:parasitology / Q000473:pathology; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008168:Lung / Q000469:parasitology / Q000473:pathology; D008297:Male; D011233:Precipitin Tests; D060888:Real-Time Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000235:genetics / Q000302:isolation & purification*; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000473:pathology; D012680:Sensitivity and Specificity; D013154:Spleen / Q000469:parasitology / Q000473:pathology
chemical_list: D018509:DNA, Helminth
keywords: Circumoval precipitin test; Histopathology; Kato-Katz method; S. mansoni; TaqMan® Real-Time PCR system