title: Exploring the diversity of Acinetobacter populations in river water with genus-specific primers and probes.
abstract: This study aimed to explore the diversity of river water Acinetobacter populations using culture-dependent and -independent methods. Pyrosequencing indicated that 1.5% of the total sequences from Qiandeng River water were classified as Acinetobacter. Twelve Acinetobacter strains were isolated from three different sampling sites of the Qiandeng River. Based on culture-dependent methods, A. johnsonii, A. lwoffii and A. guillouiae were the most abundantly represented Acinetobacter strains among the upper, middle and downstream populations of the river. Probing of three Acinetobacter-enriched 16S rRNA gene libraries with the Acinetobacter specific probe Act660F revealed 42 unique 16S rRNA gene sequences exhibiting a similarity of 94.9-99.9% with the known Acinetobacter strains. Among the uncultured Acinetobacter sequences, 50%, 58.3% and 68.8% of those obtained from upstream sampling site A, middle stream sampling site B and downstream sampling site C were phylogenetically located within Group I. This Group represented the most abundant strains of Acinetobacter populations in river water based on culture-independent methods. The results indicated that culture-independent methods provide more detailed information on the diversity of Acinetobacter populations than that based on culture-dependent methods. Therefore, the development of new and efficient isolation methods to identify uncultured Acinetobacter species is required.
authors.affiliation: School of Life Science, East China Normal University.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Xin
authors.forename: Dijie
authors.initials: D
authors.lastname: Cai
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Sun
authors.forename: Dalei
authors.initials: D
authors.lastname: Guo
authors.forename: Zirong
authors.initials: Z
authors.lastname: Wu
authors.forename: Deming
authors.initials: D
authors.lastname: Jiang
mesh_terms: D000150:Acinetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D016000:Cluster Analysis; D017931:DNA Primers; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S