title: Cultivation of nitrite-dependent anaerobic methane-oxidizing bacteria: impact of reactor configuration.
abstract: Nitrite-dependent anaerobic methane oxidation (n-damo) is mediated by bacteria that anaerobically oxidize methane coupled with nitrite reduction and is a potential bioprocess for wastewater treatment. In this work, the effect of reactor configuration on n-damo bacterial cultivation was investigated. A magnetically stirred gas lift reactor (MSGLR), a sequencing batch reactor (SBR), and a continuously stirred tank reactor (CSTR) were selected to cultivate the bacteria. Microbial community was monitored by using quantitative PCR, 16S rRNA gene sequencing, pmoA gene sequencing, and fluorescence in situ hybridization (FISH). The effects of substrate inhibition, methane mass transfer, and biomass washout in the three reactors were focused on. The results indicated that the MSGLR had the best performance among the three reactor systems, with the highest total and specific n-damo activities. Its maximum volumetric nitrogen removal rate was up to 76.9 mg N L(-1) day(-1), which was higher than previously reported values (5.1-37.8 mg N L(-1) d(-1)).
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Baolan
authors.initials: B
authors.lastname: Hu
authors.forename: Zhanfei
authors.initials: Z
authors.lastname: He
authors.forename: Sha
authors.initials: S
authors.lastname: Geng
authors.forename: Chen
authors.initials: C
authors.lastname: Cai
authors.forename: Liping
authors.initials: L
authors.lastname: Lou
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.forename: Xinhua
authors.initials: X
authors.lastname: Xu
mesh_terms: D000693:Anaerobiosis; D019149:Bioreactors / Q000382:microbiology; D017404:In Situ Hybridization, Fluorescence; D008772:Methylococcaceae / Q000235:genetics / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D009573:Nitrites; D012336:RNA, Ribosomal, 16S