title: [The degree of chronic renal failure is associated with the rate of pro-inflammatory cytokines, hyperhomocysteinemia and with oxidative stress].
abstract: OBJECTIVE
  To evaluate pro-inflammatory cytokines, homocysteinemia and markers of oxidative status in the course of chronic renal failure.
  
  
  METHODS
  One hundred and two patients (male/female: 38/64; age: 45±07 years) with chronic renal failure were divided into 4 groups according to the National Kidney Foundation classification. They included 28 primary stage renal failure patients, 28 moderate stage renal failure, 28 severe stage renal failure and 18 end stage renal failure. The inflammatory status was evaluated by the determination of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β, interleukin-6) and total homocysteine. Pro-oxidant status was assessed by assaying thiobarbituric acid reactive substances, hydroperoxides, and protein carbonyls. Antioxidant defence was performed by analysis of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase.
  
  
  RESULTS
  Inflammatory markers were elevated in the end stage renal failure group compared to the other groups (P<0.001). Indeed, an increase in thiobarbituric acid reactive substances, hydroperoxides and protein carbonyls was noted in the end stage renal failure group in comparison with the other groups (P<0.001), while the levels of antioxidants enzymes activity were decreased in the study population (P<0.001).
  
  
  CONCLUSIONS
  Impaired renal function is closely associated with the elevation of inflammatory markers leading to both increased markers of oxidative stress and decreased antioxidant defense.
authors.affiliation: Laboratoire de nutrition clinique et métabolique, faculté des sciences de la nature et de la vie, université d'Oran, Oran 31100, Algérie.
authors.forename: H F
authors.initials: HF
authors.lastname: Tbahriti
authors.affiliation: Service de biochimie, établissement hospitalier universitaire (EHU) d'Oran, Oran 31037, Algérie.
authors.forename: A
authors.initials: A
authors.lastname: Messaoudi
authors.affiliation: Service de nephrologie, établissement hospitalier universitaire (EHU) d'Oran, Oran 31037, Algérie.
authors.forename: A
authors.initials: A
authors.lastname: Kaddous
authors.affiliation: Laboratoire de nutrition clinique et métabolique, faculté des sciences de la nature et de la vie, université d'Oran, Oran 31100, Algérie.
authors.forename: M
authors.initials: M
authors.lastname: Bouchenak
authors.affiliation: Laboratoire de nutrition clinique et métabolique, faculté des sciences de la nature et de la vie, université d'Oran, Oran 31100, Algérie. Electronic address: khmekki@hotmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Mekki
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D016207:Cytokines / Q000097:blood*; D005260:Female; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007676:Kidney Failure, Chronic / Q000097:blood / Q000175:diagnosis* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015415:Biomarkers; D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D006710:Homocysteine
keywords: Chronic renal failure; Cytokines pro-inflammatoires; Homocystéine totale; Insuffisance rénale chronique; Oxidative status; Pro-inflammatory cytokines; Statut oxydant/antioxydant; Total homocysteine