title: Laboratory evaluation of different agar media for isolation of carbapenem-resistant Acinetobacter spp.
abstract: The optimal method for surveillance of carbapenem-resistant Acinetobacter spp. (CRAB) is unknown. A collection of CRAB strains (n = 42), carbapenem-susceptible strains (CSAB), and non-Acinetobacter strains (n = 18) was used to evaluate six laboratory surveillance methods: MacConkey (MAC), MAC + 1 μg/ml imipenem (MAC-IPM), minimal salts agar + 1 % acetate (MSA), MSA with IPM disk (MSA-IPM), CHROMagarKPC, and CHROMagar Acinetobacter with CR102 (CHROMAcineto). CHROMAcineto was 100 % sensitive and specific. CHROMagarKPC and MAC-IPM were highly sensitive (>95 %), but their specificity was substantially hampered by the breakthrough growth of CSAB. MSA was unsuitable for CRAB detection. CHROMAcineto is a promising medium for CRAB detection and warrants further clinical evaluation.
authors.affiliation: Public Health Services, Ministry of Health, 39 Yermiyahu Street, Jerusalem, Israel, giladko@post.bgu.ac.il.
authors.forename: J
authors.initials: J
authors.lastname: Moran-Gilad
authors.forename: A
authors.initials: A
authors.lastname: Adler
authors.forename: D
authors.initials: D
authors.lastname: Schwartz
authors.forename: S
authors.initials: S
authors.lastname: Navon-Venezia
authors.forename: Y
authors.initials: Y
authors.lastname: Carmeli
mesh_terms: D000150:Acinetobacter / Q000302:isolation & purification*; D000151:Acinetobacter Infections / Q000175:diagnosis*; D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods*; D015780:Carbapenems / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D012680:Sensitivity and Specificity; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D003470:Culture Media; D000362:Agar