title: Effect of natural porcine surfactant in Staphylococcus aureus induced pro-inflammatory cytokines and reactive oxygen species generation in monocytes and neutrophils from human blood.
abstract: Surfacen® is a clinical surfactant preparation of porcine origin. In the present study, we have evaluated the effect of Surfacen® in the modulation of oxidative burst in monocytes and neutrophils in human blood and pro-inflammatory cytokine production in peripheral blood mononuclear cells (PBMC). Reactive oxygen species (ROS) level was measured in monocytes and neutrophils by flow cytometry using 2,7-dichlorofluorescein diacetate (DCFH-DA) as substrate, while, tumor necrosis factor (TNF)-α and interleukin (IL)-6 levels were estimated in PBMC supernatant by enzyme-linked immunosorbent assays (ELISA). Our results show that Staphylococcus aureus-induced ROS level was slightly affected by Surfacen® added to whole blood monocytes and neutrophils. The time course experiments of pre-incubation with Surfacen® showed no significant increase of ROS level at 2h; however, the ROS level decreased when pre incubated for 4h and 6h with Surfacen®. Pre-incubation of PBMC cells with Surfacen® at 0.125 and 0.5mg/mL showed a dose-dependent suppression of TNF-α levels measured after 4h of S. aureus stimulation, an effect less impressive when cells were stimulated for 24h. A similar behavior was observed in IL-6 release. In summary, the present study provides experimental evidence supporting an anti-inflammatory role of Surfacen® in human monocytes and neutrophils in vitro.
authors.affiliation: Centro Nacional de Sanidad Agropecuaria (CENSA), San José de las Lajas, Mayabeque, Cuba.
authors.forename: Yuliannis Lugones Ladrón
authors.initials: YL
authors.lastname: de Guevara
authors.affiliation: Centro Nacional de Sanidad Agropecuaria (CENSA), San José de las Lajas, Mayabeque, Cuba.
authors.forename: Odalys Blanco
authors.initials: OB
authors.lastname: Hidalgo
authors.affiliation: Division of Infectious Diseases, Hospital São Paulo, Escola Paulista de Medicina, Universidade Federal de São Paulo, Brazil.
authors.forename: Sidnéia Sousa
authors.initials: SS
authors.lastname: Santos
authors.affiliation: Division of Infectious Diseases, Hospital São Paulo, Escola Paulista de Medicina, Universidade Federal de São Paulo, Brazil.
authors.forename: Milena Karina Colo
authors.initials: MK
authors.lastname: Brunialti
authors.affiliation: Centro Nacional de Sanidad Agropecuaria (CENSA), San José de las Lajas, Mayabeque, Cuba.
authors.forename: Roberto
authors.initials: R
authors.lastname: Faure
authors.affiliation: Division of Infectious Diseases, Hospital São Paulo, Escola Paulista de Medicina, Universidade Federal de São Paulo, Brazil. Electronic address: rsalomao@unifesp.br.
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Salomao
mesh_terms: D000328:Adult; D000818:Animals; D016207:Cytokines / Q000276:immunology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010743:Phospholipids / Q000276:immunology / Q000494:pharmacology; D037661:Pulmonary Surfactant-Associated Proteins / Q000276:immunology / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000276:immunology*; D016897:Respiratory Burst / Q000187:drug effects / Q000276:immunology; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology*; D013501:Surface-Active Agents / Q000494:pharmacology*; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D010743:Phospholipids; D037661:Pulmonary Surfactant-Associated Proteins; D017382:Reactive Oxygen Species; D013501:Surface-Active Agents; D014409:Tumor Necrosis Factor-alpha; C569617:surfacen
keywords: IL-6; Inflammation; Pulmonary surfactant; ROS; Surfacen®; TNF-α