title: New tools for embryo selection: comprehensive chromosome screening by array comparative genomic hybridization.
abstract: The objective of this study was to evaluate the usefulness of comprehensive chromosome screening (CCS) using array comparative genomic hybridization (aCGH). The study included 1420 CCS cycles for recurrent miscarriage (n = 203); repetitive implantation failure (n = 188); severe male factor (n = 116); previous trisomic pregnancy (n = 33); and advanced maternal age (n = 880). CCS was performed in cycles with fresh oocytes and embryos (n = 774); mixed cycles with fresh and vitrified oocytes (n = 320); mixed cycles with fresh and vitrified day-2 embryos (n = 235); and mixed cycles with fresh and vitrified day-3 embryos (n = 91). Day-3 embryo biopsy was performed and analyzed by aCGH followed by day-5 embryo transfer. Consistent implantation (range: 40.5-54.2%) and pregnancy rates per transfer (range: 46.0-62.9%) were obtained for all the indications and independently of the origin of the oocytes or embryos. However, a lower delivery rate per cycle was achieved in women aged over 40 years (18.1%) due to the higher percentage of aneuploid embryos (85.3%) and lower number of cycles with at least one euploid embryo available per transfer (40.3%). We concluded that aneuploidy is one of the major factors which affect embryo implantation.
authors.affiliation: IVIOMICS SPAIN, Parc Cientific Universitat de València, Calle Catedrático Agustín Escardino 9, 46980 Valencia, Spain ; Instituto Valenciano de Infertilidad (IVI), Instituto Universitario IVI/INCLIVA, Plaza de la Policía Local 3, 46015 Valencia, Spain.
authors.forename: Lorena
authors.identifier: 0000-0003-4133-9812
authors.initials: L
authors.lastname: Rodrigo
authors.affiliation: IVIOMICS SPAIN, Parc Cientific Universitat de València, Calle Catedrático Agustín Escardino 9, 46980 Valencia, Spain ; Instituto Valenciano de Infertilidad (IVI), Instituto Universitario IVI/INCLIVA, Plaza de la Policía Local 3, 46015 Valencia, Spain.
authors.forename: Emilia
authors.identifier: 0000-0003-0838-9981
authors.initials: E
authors.lastname: Mateu
authors.affiliation: Instituto Valenciano de Infertilidad (IVI), Instituto Universitario IVI/INCLIVA, Plaza de la Policía Local 3, 46015 Valencia, Spain.
authors.forename: Amparo
authors.identifier: 0000-0002-5476-3703
authors.initials: A
authors.lastname: Mercader
authors.affiliation: Instituto Valenciano de Infertilidad (IVI), Instituto Universitario IVI/INCLIVA, Plaza de la Policía Local 3, 46015 Valencia, Spain.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Cobo
authors.affiliation: IVIOMICS SPAIN, Parc Cientific Universitat de València, Calle Catedrático Agustín Escardino 9, 46980 Valencia, Spain.
authors.forename: Vanessa
authors.identifier: 0000-0003-3788-7728
authors.initials: V
authors.lastname: Peinado
authors.affiliation: IVIOMICS SPAIN, Parc Cientific Universitat de València, Calle Catedrático Agustín Escardino 9, 46980 Valencia, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Milán
authors.affiliation: IVIOMICS USA, 1200 NW 78th Avenue, Suite 103, Miami, FL 33126, USA.
authors.forename: Nasser
authors.initials: N
authors.lastname: Al-Asmar
authors.affiliation: IVIOMICS SPAIN, Parc Cientific Universitat de València, Calle Catedrático Agustín Escardino 9, 46980 Valencia, Spain.
authors.forename: Inmaculada
authors.identifier: 0000-0003-3961-8628
authors.initials: I
authors.lastname: Campos-Galindo
authors.affiliation: IVIOMICS SPAIN, Parc Cientific Universitat de València, Calle Catedrático Agustín Escardino 9, 46980 Valencia, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: García-Herrero
authors.affiliation: IVIOMICS INDIA, 13 Olof Palme Marg, Vasant Vihar, New Delhi 11057, India.
authors.forename: Pere
authors.initials: P
authors.lastname: Mir
authors.affiliation: IVIOMICS SPAIN, Parc Cientific Universitat de València, Calle Catedrático Agustín Escardino 9, 46980 Valencia, Spain ; Instituto Valenciano de Infertilidad (IVI), Instituto Universitario IVI/INCLIVA, Plaza de la Policía Local 3, 46015 Valencia, Spain ; Fundación IVI, 46980 Valencia, Spain ; Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
authors.affiliation: IVIOMICS SPAIN, Parc Cientific Universitat de València, Calle Catedrático Agustín Escardino 9, 46980 Valencia, Spain ; Instituto Valenciano de Infertilidad (IVI), Instituto Universitario IVI/INCLIVA, Plaza de la Policía Local 3, 46015 Valencia, Spain.
authors.forename: Carmen
authors.identifier: 0000-0002-7582-3875
authors.initials: C
authors.lastname: Rubio
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics* / Q000473:pathology; D000328:Adult; D055028:Comparative Genomic Hybridization / Q000295:instrumentation* / Q000379:methods*; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D009865:Oocytes / Q000473:pathology; D011247:Pregnancy; D014314:Trisomy / Q000235:genetics* / Q000473:pathology