title: Age-dependent dopaminergic dysfunction following fetal exposure to atrazine in SD rats.
abstract: The herbicide, atrazine (ATR), is used worldwide and its contamination in the environment has resulted in documented human exposure. It has also been shown that ATR results in dopaminergic neurotoxicity, however, few studies have investigated the long-term effects of ATR following in utero exposure. Therefore, we evaluated the effects of ATR exposure in Sprague Dawley rats during gestational on the offspring dopaminergic system development. Pregnant dams were treated with oral ATR at 0, 25, 50 mg/kg/day from gestational day 0 to postnatal day 1. In this study, we examined the hypothesis that ATR could cross the placental barrier and have long-term adverse effects on the synthesis, degradation and reuptake of DA in the brain. For this purpose,we examine the concentration of levodopa (L-DA), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in stratum. The mRNA and protein expression of orphan nuclear hormone (Nurr1), tyrosine hydroxylase(TH), vesicular monoaminetransporter 2 (VMAT2), dopamine transporter (DAT), monoamine (MAO), and catechol-O-methyl transferase (COMT) in the midbrain were examined by fluorescence PCR and Western blot when the offspring reached six-month old or one year old .When measured 6 months post-treatment, the level of DA and expression of Nurr1, VMAT2, DAT and TH were reduced in the striatum and Substantia nigra, respectively.
authors.affiliation: Department of Toxicology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province 150081, PR China. Electronic address: liyan.shu@hotmail.com.
authors.forename: Yanshu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Toxicology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province 150081, PR China. Electronic address: 171529406@qq.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Toxicology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province 150081, PR China. Electronic address: 736304908@qq.com.
authors.forename: Junwei
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Toxicology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province 150081, PR China. Electronic address: 1814656802@qq.com.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Toxicology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province 150081, PR China. Electronic address: pengchen21@163.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Toxicology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province 150081, PR China. Electronic address: libaixiang_hmu@163.com.
authors.forename: Baixiang
authors.initials: B
authors.lastname: Li
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000375:Aging / Q000502:physiology; D000818:Animals; D001280:Atrazine / Q000633:toxicity*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D050483:Dopamine Plasma Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005333:Fetus; D006540:Herbicides / Q000633:toxicity*; D006719:Homovanillic Acid / Q000378:metabolism; D007980:Levodopa / Q000378:metabolism; D008431:Maternal-Fetal Exchange; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2 / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000187:drug effects* / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism; D050493:Vesicular Monoamine Transport Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; D006540:Herbicides; C539884:Nr4a2 protein, rat; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D012333:RNA, Messenger; C493454:Slc18a2 protein, rat; D050493:Vesicular Monoamine Transport Proteins; D015102:3,4-Dihydroxyphenylacetic Acid; D007980:Levodopa; D014446:Tyrosine 3-Monooxygenase; D001280:Atrazine; D004298:Dopamine; D006719:Homovanillic Acid
keywords: Aging; Atrazine; Neurotoxicity; Parkinson disease