title: The ontogenesis of the dopaminergic cell in the pre- and postnatal guinea pig retina.
abstract: We have examined the development of the dopaminergic system of the guinea pig retina, a species in which retinal neuronal and synaptic differentiation occurs largely in utero. Fetal animals aged 42-69 days (full term), neonates, postnatal (pn) animals to 12 weeks, and mature animals were studied to determine retinal dopamine (DA) storage, metabolism (DOPAC), in vitro tyrosine hydroxylase (TH) activity, postsynaptic target activation (cAMP stimulation) and localization (formaldehyde-induced histofluorescence). DA-stimulated adenylate cyclase at 42 days of gestation was threefold over basal activity, preceding the onset of the accumulation of DA and DOPAC at 45 days, and the initial localization of DA in cell perikarya at 47 days and in processes at 50 days. At birth DA and DOPAC levels were 45 and 37%, respectively, of adult levels. DA levels remained stable during the first few days pn, although in vitro TH activity was capable of stimulation by light in the neonate as in the mature animal. DA and TH activity increased from 1 week pn to reach adult levels by 10 weeks pn. Although a significant degree of development of the dopaminergic neurotransmitter system in the guinea pig occurs before birth the attainment of a fully mature system postnatally may require normal photic stimulation of physiologic activity.
authors.affiliation: Department of Anatomy and Lions' Sight Centre, The University of Calgary, Calgary, Alberta, Canada T2N 4N1.
authors.forename: D
authors.initials: D
authors.lastname: Parkinson
authors.forename: A
authors.initials: A
authors.lastname: Spira
authors.forename: J P
authors.initials: JP
authors.lastname: Wyse
authors.forename: M
authors.initials: M
authors.lastname: Patten