title: Electropolymerized carbonic anhydrase immobilization for carbon dioxide capture.
abstract: Biomimetic carbonation carried out with carbonic anhydrase (CA) in CO2-absorbing solutions, such as methyldiethanolamine (MDEA), is one approach that has been developed to accelerate the capture of CO2. However, there are several practical issues, such as high cost and limited enzyme stability, that need to be overcome. In this study, the capacity of CA immobilization on a porous solid support was studied to improve the instability in the tertiary amine solvent. We have shown that a 63% porosity macroporous carbon foam support makes separation and reuse facile and allows for an efficient supply and presentation of CO2 to an aqueous solvent and the enzyme catalytic center. These enzymatic supports conserved 40% of their initial activity after 42 days at 70 °C in an amine solvent, whereas the free enzyme shows no activity after 1 h in the same conditions. In this work, we have overcome the technical barrier associated with the recovery of the biocatalyst after operation, and most of all, these electropolymerized enzymatic supports have shown a remarkable increase of thermal stability in an amine-based CO2 sequestration solvent.
authors.affiliation: Faculty of Dentistry, and §Division of Orthopedics, Department of Surgery, Faculty of Medicine, McGill University , Montreal, Quebec H3A 0C7, Canada.
authors.forename: Geraldine
authors.initials: G
authors.lastname: Merle
authors.forename: Sylvie
authors.initials: S
authors.lastname: Fradette
authors.forename: Eric
authors.initials: E
authors.lastname: Madore
authors.forename: Jake E
authors.initials: JE
authors.lastname: Barralet
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry*; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004983:Ethanolamines / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D004983:Ethanolamines; D002245:Carbon Dioxide; C008430:N-methyldiethanolamine; D002256:Carbonic Anhydrases