title: A new saikogenin from the roots of Bupleurum bicaule.
abstract: OBJECTIVE
  To study the chemical constituents from the roots of Buleurum bicaule Helm (Apiaceae).
  
  
  METHODS
  Silica gel, Sephadex LH-20, MPLC Rp-C18 column chromatography, and HPLC were used for isolation of compounds. The structures were elucidated on the basis of 1D- and 2D-NMR technology and HRESI-MS. Compounds were evaluated in vitro for their inhibitory ability against the proliferation of rat mesangial cells by the MTT method.
  
  
  RESULTS
  Twelve compounds were isolated, and their structures were identified on the basis of their spectroscopic and physico-chemical properties as 13, 28-epoxy-olean-11-en-3-one (1), saikogenin E (2), saikogenin G (3), 11α-methoxy-3β, 16β, 23, 28-tetrahydroxyolean-12-ene (4), saikogenin D (5), prosaikogenin F (6), prosaikogenin A (7), prosaikogenin G (8), prosaikogenin D (9), laccaic acid (10b), methyl gallate (11), and ethyl gallate (12). Compounds 1, 2, 7, 8, and 10 were observed to have inhibitory activity against mesangial cell proliferationin to different degrees.
  
  
  CONCLUSIONS
  Compound 1, 8, and 10 exhibit significant inhibitory effects on rat mesangial cell proliferation induced by Ang II.
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Xu
authors.affiliation: Dalian Statute of Drug and Food examination, Dalian 116101, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Shi
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhong
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cao
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. Electronic address: jiatz@126.com.
authors.forename: Tian-Zhu
authors.initials: TZ
authors.lastname: Jia
mesh_terms: D000818:Animals; D029972:Bupleurum / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D014315:Triterpenes
keywords: Anti-proliferation; Bupleurum bicaule; Saikogenins; Triterpenoid saponins