title: Snx14 regulates neuronal excitability, promotes synaptic transmission, and is imprinted in the brain of mice.
abstract: Genomic imprinting describes an epigenetic process through which genes can be expressed in a parent-of-origin-specific manner. The monoallelic expression of imprinted genes renders them particularly susceptible to disease causing mutations. A large proportion of imprinted genes are expressed in the brain, but little is known about their functions. Indeed, it has proven difficult to identify cell type-specific imprinted genes due to the heterogeneity of cell types within the brain. Here we used laser capture microdissection of visual cortical neurons and found evidence that sorting nexin 14 (Snx14) is a neuronally imprinted gene in mice. SNX14 protein levels are high in the brain and progressively increase during neuronal development and maturation. Snx14 knockdown reduces intrinsic excitability and severely impairs both excitatory and inhibitory synaptic transmission. These data reveal a role for monoallelic Snx14 expression in maintaining normal neuronal excitability and synaptic transmission.
authors.affiliation: Department of Cell Biology & Physiology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America.
authors.forename: Hsien-Sung
authors.initials: HS
authors.lastname: Huang
authors.affiliation: Division of Life Sciences, Korea University, Seoul, Korea.
authors.forename: Bong-June
authors.initials: BJ
authors.lastname: Yoon
authors.affiliation: Department of Cell Biology & Physiology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America.
authors.forename: Sherian
authors.initials: S
authors.lastname: Brooks
authors.affiliation: Department of Cell Biology & Physiology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America.
authors.forename: Robert
authors.initials: R
authors.lastname: Bakal
authors.affiliation: Curriculum in Neurobiology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, Unites States of America.
authors.forename: Janet
authors.initials: J
authors.lastname: Berrios
authors.affiliation: Department of Cell Biology & Physiology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America.
authors.forename: Rylan S
authors.initials: RS
authors.lastname: Larsen
authors.affiliation: Curriculum in Neurobiology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, Unites States of America.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Wallace
authors.affiliation: Department of Cell Biology & Physiology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Han
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America.
authors.forename: Eui Hwan
authors.initials: EH
authors.lastname: Chung
authors.affiliation: Department of Cell Biology & Physiology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America; Curriculum in Neurobiology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, Unites States of America; Neuroscience Center, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America; Carolina Institute for Developmental Disabilities, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Zylka
authors.affiliation: Department of Cell Biology & Physiology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America; Curriculum in Neurobiology, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, Unites States of America; Neuroscience Center, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America; Carolina Institute for Developmental Disabilities, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina, United States of America.
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Philpot
mesh_terms: D000818:Animals; D018392:Genomic Imprinting / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D058305:Sorting Nexins / Q000235:genetics / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*; D014793:Visual Cortex / Q000166:cytology / Q000378:metabolism*
chemical_list: C437950:Snx14 protein, mouse; D058305:Sorting Nexins