title: Re-print of "Histone extraction protocol from the two model diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana".
abstract: Post-translational modifications of histones affect many biological processes by influencing higher order chromatin structure that affects gene and genome regulation. It is therefore important to develop methods for extracting histones while maintaining their native post-translational modifications. While histone extraction protocols have been developed in multicellular and single celled organisms such as yeast and Arabidopsis, they are inefficient in diatoms that have a silica cell wall that is likely to hinder histone extraction. We report in this work a rapid and reliable method for extraction of large amounts of high quality histones from the two model diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana. The protocol is an important enabling step permitting downstream applications such as western blotting and mass spectrometry.
authors.affiliation: Environmental and Evolutionary Genomics Section, Institut de Biologie de l'École Normale Supérieure (IBENS), CNRS UMR 8197 INSERM U1024, 46 rue d'Ulm 75005 Paris, France. Electronic address: tirichin@biologie.ens.fr.
authors.forename: Leïla
authors.initials: L
authors.lastname: Tirichine
authors.affiliation: Environmental and Evolutionary Genomics Section, Institut de Biologie de l'École Normale Supérieure (IBENS), CNRS UMR 8197 INSERM U1024, 46 rue d'Ulm 75005 Paris, France.
authors.forename: Xin
authors.initials: X
authors.lastname: Lin
authors.affiliation: Environmental and Evolutionary Genomics Section, Institut de Biologie de l'École Normale Supérieure (IBENS), CNRS UMR 8197 INSERM U1024, 46 rue d'Ulm 75005 Paris, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Thomas
authors.affiliation: Institut Curie, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, 26 rue d'Ulm 75248 Cedex 05 Paris, France.
authors.forename: Bérangère
authors.initials: B
authors.lastname: Lombard
authors.affiliation: Institut Curie, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, 26 rue d'Ulm 75248 Cedex 05 Paris, France.
authors.forename: Damarys
authors.initials: D
authors.lastname: Loew
authors.affiliation: Environmental and Evolutionary Genomics Section, Institut de Biologie de l'École Normale Supérieure (IBENS), CNRS UMR 8197 INSERM U1024, 46 rue d'Ulm 75005 Paris, France.
authors.forename: Chris
authors.initials: C
authors.lastname: Bowler
keywords: Epigenetics; Mass spectrometry; Phaeodactylum tricornutum; Post-translational histone modifications; Thalassiosira pseudonana