title: Human periodontal ligament derived progenitor cells: effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior.
abstract: OBJECTIVE
  STRO-1 positive periodontal ligament cells (PDLCs) and unsorted PDLCs have demonstrated potential for periodontal regeneration, but the comparison between unsorted cells and the expanded STRO-1 sorted cells has never been reported. Additionally, Wnt3a is involved in cell proliferation thus may benefit in vitro PDLC expansion. The aim was to evaluate the effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior of human PDLCs (hPDLCs).
  
  
  METHODS
  STRO-1 positive hPDLCs were sorted and the sorted cells were expanded and compared with their unsorted parental cells. Thereafter, hPDLCs were treated with or without Wnt3a and the cell proliferation, self-renewal, and osteogenic differentiation were evaluated.
  
  
  RESULTS
  No differences were measured between the expanded STRO-1-sorted cells and unsorted parental cells in terms of proliferation, CFU, and mineralization capacity. Wnt3a enhanced the proliferation and self-renewal ability of hPDLCs significantly as displayed by higher DNA content values, a shorter cell population doubling time, and higher expression of the self-renewal gene Oct4. Moreover, Wnt3a promoted the expansion of hPDLCs for 5 passages without affecting cell proliferation, CFU, and osteogenic capacity.
  
  
  CONCLUSIONS
  Expanded STRO-1-sorted hPDLCs showed no superiority compared to their unsorted parental cells. On the other hand, Wnt3a promotes the efficient hPDLC expansion and retains the self-renewal and osteogenic differentiation capacity.
authors.affiliation: Department of Periodontology and Biomaterials, Radboud University Medical Center, 309 Dentistry, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands ; Department of Periodontology, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Xiang-Zhen
authors.initials: XZ
authors.lastname: Yan
authors.affiliation: Department of Periodontology and Biomaterials, Radboud University Medical Center, 309 Dentistry, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: Sanne K
authors.initials: SK
authors.lastname: Both
authors.affiliation: Department of Periodontology, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Pi-Shan
authors.initials: PS
authors.lastname: Yang
authors.affiliation: Department of Periodontology and Biomaterials, Radboud University Medical Center, 309 Dentistry, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.affiliation: Department of Periodontology and Biomaterials, Radboud University Medical Center, 309 Dentistry, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: Jeroen J J P
authors.initials: JJ
authors.lastname: van den Beucken
authors.affiliation: Department of Periodontology and Biomaterials, Radboud University Medical Center, 309 Dentistry, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
mesh_terms: D000328:Adult; D000954:Antigens, Surface / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects; D010513:Periodontal Ligament / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D060509:Wnt3A Protein / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D000954:Antigens, Surface; C525591:STRO-1 antigen, human; D060509:Wnt3A Protein