title: Sinosporangium siamense sp. nov., isolated from soil and emended description of the genus Sinosporangium.
abstract: An actinomycetes strain A-T 1946(T) that developed spherical sporangia containing non-motile spores on aerial mycelia was isolated from dry deciduous forest soil in Thailand. 16S rRNA gene sequence analysis indicated that strain A-T 1946(T) belongs to the genus Sinosporangium, being closely related to Sinosporangium album 6014(T) (98.8% sequence similarity). The DNA-DNA relatedness values were 43.7-50.9%, which were significantly below 70% and differentiated strain A-T 1946(T) from the closest species. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, madurose and glucose. The predominant menaquinone was MK-9(H2). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, lysophosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-mannoside, N-acetylglucosamine-containing phospholipids, two unknown phosphoglycolipids and two unknown phospholipids. The predominant cellular fatty acids were unsaturated C(16 : 1) and C(17 : 1), and saturated C(16 : 0) and 10-methyl-C(17 : 0). Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the isolate is proposed to represent a novel species of genus Sinosporangium to be named Sinosporangium siamense sp. nov. The type strain is A-T 1946(T) ( = BCC 29081(T) = NBRC 109515(T)). An emended description of the genus Sinosporangium is also provided.
authors.affiliation: BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand.|Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Chanwit
authors.initials: C
authors.lastname: Suriyachadkun
authors.affiliation: BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand.
authors.forename: Wipaporn
authors.initials: W
authors.lastname: Ngaemthao
authors.affiliation: BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand.
authors.forename: Suwanee
authors.initials: S
authors.lastname: Chunhametha
authors.affiliation: Department of Biology and Actinobacterial Research Unit, Faculty of Science, King Monkut's Institute of Technology Ladkrabang, Bangkok, Thailand.
authors.forename: Chitti
authors.initials: C
authors.lastname: Thawai
authors.affiliation: BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Sanglier
authors.affiliation: Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Vichien
authors.initials: V
authors.lastname: Kitpreechavanich
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013785:Thailand; D014197:Trees; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid