title: Evaluation of the total MBL confirm kit (ROSCO) for detection of metallo-β-lactamases in Pseudomonas aeruginosa and Acinetobacter baumannii.
abstract: Phenotypic tests for carbapenemase production in Pseudomonas aeruginosa and Acinetobacter baumannii have been associated with unspecific metallo-β-lactamase (MBL) inhibitor activity in synergy tests and low positive predictive value. In this study, a collection of well-characterized P. aeruginosa and A. baumannii isolates was used to evaluate the inhibitor-based Total MBL Confirm Kit and the MBL Etest.
authors.affiliation: Statens Serum Institut, Copenhagen, Denmark. Electronic address: fha@ssi.dk.
authors.forename: Frank
authors.initials: F
authors.lastname: Hansen
authors.affiliation: Statens Serum Institut, Copenhagen, Denmark.
authors.forename: Anette M
authors.initials: AM
authors.lastname: Hammerum
authors.affiliation: Statens Serum Institut, Copenhagen, Denmark.
authors.forename: Robert
authors.initials: R
authors.lastname: Skov
authors.affiliation: University Hospital of North Norway, Tromsø, Norway.
authors.forename: Bjørg
authors.initials: B
authors.lastname: Haldorsen
authors.affiliation: University Hospital of North Norway, Tromsø, Norway.
authors.forename: Arnfinn
authors.initials: A
authors.lastname: Sundsfjord
authors.affiliation: University Hospital of North Norway, Tromsø, Norway.
authors.forename: Orjan
authors.initials: O
authors.lastname: Samuelsen
mesh_terms: D000151:Acinetobacter Infections / Q000175:diagnosis* / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000175:diagnosis* / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D011933:Reagent Kits, Diagnostic; D001618:beta-Lactamases
keywords: Acinetobacter baumannii; Dipicolinic acid; Metallo-β-lactamase inhibitors; Phenotypic tests; Pseudomonas aeruginosa