title: Characterization of human αβTCR repertoire and discovery of D-D fusion in TCRβ chains.
abstract: The characterization of the human T-cell receptor (TCR) repertoire has made remarkable progress, with most of the work focusing on the TCRβ chains. Here, we analyzed the diversity and complexity of both the TCRα and TCRβ repertoires of three healthy donors. We found that the diversity of the TCRα repertoire is higher than that of the TCRβ repertoire, whereas the usages of the V and J genes tended to be preferential with similar TRAV and TRAJ patterns in all three donors. The V-J pairings, like the V and J gene usages, were slightly preferential. We also found that the TRDV1 gene rearranges with the majority of TRAJ genes, suggesting that TRDV1 is a shared TRAV/DV gene (TRAV42/DV1). Moreover, we uncovered the presence of tandem TRBD (TRB D gene) usage in ~2% of the productive human TCRβ CDR3 sequences.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China, peipei.liu@hotmail.com.
authors.forename: Peipei
authors.initials: P
authors.lastname: Liu
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Xue
authors.initials: X
authors.lastname: Xiao
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zou
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Juncai
authors.initials: J
authors.lastname: Ma
authors.forename: Fangqing
authors.initials: F
authors.lastname: Zhao
authors.forename: Xuyu
authors.initials: X
authors.lastname: Zhou
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
mesh_terms: D022801:Complementarity Determining Regions / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D005260:Female; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000235:genetics; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor / Q000235:genetics*; D019674:Genes, T-Cell Receptor beta / Q000235:genetics*; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008297:Male; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*
chemical_list: D022801:Complementarity Determining Regions; D017931:DNA Primers; D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region; D016693:Receptors, Antigen, T-Cell, alpha-beta