title: Highly sensitive single domain antibody-quantum dot conjugates for detection of HER2 biomarker in lung and breast cancer cells.
abstract: Despite the widespread availability of immunohistochemical and other methodologies for screening and early detection of lung and breast cancer biomarkers, diagnosis of the early stage of cancers can be difficult and prone to error. The identification and validation of early biomarkers specific to lung and breast cancers, which would permit the development of more sensitive methods for detection of early disease onset, is urgently needed. In this paper, ultra-small and bright nanoprobes based on quantum dots (QDs) conjugated to single domain anti-HER2 (human epidermal growth factor receptor 2) antibodies (sdAbs) were applied for immunolabeling of breast and lung cancer cell lines, and their performance was compared to that of anti-HER2 monoclonal antibodies conjugated to conventional organic dyes Alexa Fluor 488 and Alexa Fluor 568. The sdAbs-QD conjugates achieved superior staining in a panel of lung cancer cell lines with differential HER2 expression. This shows their outstanding potential for the development of more sensitive assays for early detection of cancer biomarkers.
authors.affiliation: School of Medicine, Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College , Dublin 8, Ireland.
authors.forename: Tatsiana Y
authors.initials: TY
authors.lastname: Rakovich
authors.forename: Omar K
authors.initials: OK
authors.lastname: Mahfoud
authors.forename: Bashir M
authors.initials: BM
authors.lastname: Mohamed
authors.forename: Adriele
authors.initials: A
authors.lastname: Prina-Mello
authors.forename: Kieran
authors.initials: K
authors.lastname: Crosbie-Staunton
authors.forename: Tina
authors.initials: T
authors.lastname: Van Den Broeck
authors.forename: Line
authors.initials: L
authors.lastname: De Kimpe
authors.forename: Alyona
authors.initials: A
authors.lastname: Sukhanova
authors.forename: Daniel
authors.initials: D
authors.lastname: Baty
authors.forename: Aliaksandra
authors.initials: A
authors.lastname: Rakovich
authors.forename: Stefan A
authors.initials: SA
authors.lastname: Maier
authors.forename: Frauke
authors.initials: F
authors.lastname: Alves
authors.forename: Frans
authors.initials: F
authors.lastname: Nauwelaers
authors.forename: Igor
authors.initials: I
authors.lastname: Nabiev
authors.forename: Patrick
authors.initials: P
authors.lastname: Chames
authors.forename: Yuri
authors.initials: Y
authors.lastname: Volkov
mesh_terms: D000906:Antibodies / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D018613:Microscopy, Confocal; D045663:Quantum Dots; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human