title: [Effect of the peri-implantitis on the biological function of osteoblasts obtained from the mandibles].
abstract: OBJECTIVE
  To study the effect of peri-implantitis inflammatory microenvironment on the biological function of jaw bone osteoblasts.
  
  
  METHODS
  Primary mandible osteoblasts from peri-implantitis and normal tissue were isolated and cultured. Third-generation purified osteoblasts were identified and detected. The proliferative activity of osteoblasts was evaluated through MTT assay. Osteocalcin (OCN), Runx2, and collagen I (Col I) mRNA levels were examined by real-time quantitative polymerase chain reaction. OCN protein levels were determined by Western blot.
  
  
  RESULTS
  : After 4 d of culture, the proliferative activity of osteoblasts from peri-implantitis became lower than that of normal tissue ( P <0.05). After 7 d of culture, OCN, Runx2, and Col I mRNA expression decreased ( P <0.05). The OCN protein levels also decreased ( P <0.05).
  
  
  CONCLUSIONS
  Peri-implantitis inflammatory microenvironment can decrease the proliferation and differentiation activity of mandible osteoblasts.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Hong-Chen
authors.initials: HC
authors.lastname: Liu
authors.forename: Lingling
authors.initials: L
authors.lastname: E
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Lin
authors.initials: L
authors.lastname: Feng
mesh_terms: D001842:Bone and Bones; D002454:Cell Differentiation; D006801:Humans; D008334:Mandible; D010006:Osteoblasts; D015675:Osteocalcin; D057873:Peri-Implantitis; D012333:RNA, Messenger
chemical_list: D012333:RNA, Messenger; D015675:Osteocalcin