title: Allele and haplotype frequencies for HLA-DQ in Iranian celiac disease patients.
abstract: OBJECTIVE
  To assess the distribution of human leukocyte antigen (HLA)-DQ2 and -DQ8 in Iranian celiac disease (CD) patients and compare them to healthy Iranian controls.
  
  
  METHODS
  To predict the HLA-DQA1 and -DQB1 genes, we used six previously reported HLA-tagging single nucleotide polymorphism to determine HLA genotypes in 59 Iranian patients with 'biopsy-confirmed' CD and in 151 healthy Iranian individuals. To test the transferability of the method, 50 cases and controls were also typed using a commercial kit that identifies individual carriers of DQ2, DQ7 and DQ8 alleles.
  
  
  RESULTS
  In this pilot study 97% of CD cases (n = 57) and 58% of controls (n = 87) were carriers of HLA-DQ2 and/or HLA-DQ8 heterodimers, either in the homozygous or heterozygous state. The HLA-DQ pattern of these 57 CD patients: heterozygous DQ2.2 (n = 14) and homozygous DQ2.2 (n = 1), heterozygous DQ2.5 (n = 33) and homozygous DQ2.5 (n = 8), heterozygous DQ8 (n = 13) and homozygous DQ8 (n = 2). Two CD patients were negative for both DQ2 and DQ8 (3%).
  
  
  CONCLUSIONS
  The prevalence of DQ8 in our CD population was higher than that reported in other populations (25.4%). As reported in other populations, our results underline the primary importance of HLA-DQ alleles in the Iranian population's susceptibility to CD.
authors.affiliation: Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rostami-Nejad
authors.affiliation: Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.
authors.forename: Jihane
authors.initials: J
authors.lastname: Romanos
authors.affiliation: Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.
authors.forename: Kamran
authors.initials: K
authors.lastname: Rostami
authors.affiliation: Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.
authors.forename: Azita
authors.initials: A
authors.lastname: Ganji
authors.affiliation: Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Ehsani-Ardakani
authors.affiliation: Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.
authors.forename: Ali-Reza
authors.initials: AR
authors.lastname: Bakhshipour
authors.affiliation: Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.
authors.forename: Homayoun
authors.initials: H
authors.lastname: Zojaji
authors.affiliation: Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.
authors.forename: Seyed Reza
authors.initials: SR
authors.lastname: Mohebbi
authors.affiliation: Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Zali
authors.affiliation: Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.
authors.forename: Cisca
authors.initials: C
authors.lastname: Wijmenga
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D002446:Celiac Disease / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains / Q000235:genetics; D059866:HLA-DQ beta-Chains / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide; D015995:Prevalence; D055815:Young Adult
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C053186:HLA-DQ2 antigen; C069546:HLA-DQ7 antigen; C109756:HLA-DQ8 antigen; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen
keywords: Celiac disease; Human leukocyte antigen typing; Iran; Susceptibility; Validation