title: Paracoccus lutimaris sp. nov., isolated from a tidal flat sediment.
abstract: A Gram-negative, coccoid or oval-shaped and gliding bacterial strain, designated HDM-25(T), belonging to the Alphaproteobacteria, was isolated from a tidal flat sediment of the Yellow Sea, Korea, and was subjected to a polyphasic taxonomic study. Strain HDM-25(T) grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2-3% (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain HDM-25(T) fell within the clade comprising the species of the genus Paracoccus, clustering with the type strain of Paracoccus aminophilus, with which it exhibited the highest 16S rRNA gene sequence similarity (97.7%). The 16S rRNA gene sequence similarity between strain HDM-25(T) and the type strains of the other species of Paracoccus was 93.6-97.0%. The DNA G+C content was 65.9 mol% and the mean DNA-DNA relatedness between strain HDM-25(T) and the type strain of P. aminophilus was 10.7±2.7% (9.9±4.0%, reciprocal analysis). Strain HDM-25(T) contained Q-10 as the predominant ubiquinone and summed feature 8 (C(18 : 1)ω7c and/or C(18 : 1)ω6c) and C(16 : 0) as the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid and an unidentified lipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain HDM-25(T) is distinguishable from other species of the genus Paracoccus. On the basis of the data presented, strain HDM-25(T) is considered to represent a novel species of the genus Paracoccus, for which the name Paracoccus lutimaris sp. nov. is proposed. The type strain is HDM-25(T) ( = KCTC 42007(T) = CECT 8525(T)).
authors.affiliation: University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.|Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010230:Paracoccus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2