title: Xenopus cadherin 5 is specifically expressed in endothelial cells of the developing vascular system.
abstract: Vasculogenesis is an important, multistep process leading to the formation of a functional primary network of blood vessels in the developing embryo. A series of interactions between secreted growth factors and their specific receptors leads to the specification of mesodermal cells to become hemangioblasts, which then differentiate into angioblasts. These subsequently proliferate, coalesce into cords and finally form tubular vascular structures. For proper function of these primary blood vessels, the close connection of endothelial cells is required. This is conferred by the interaction of an endothelium specific cadherin (Cadherin-5), starting during early vascular development. However, this interaction remains important throughout life and ageing. Therefore, cadherin-5 is a useful marker for late stages of vasculogenesis in several vertebrate species. To establish cadherin-5 as a marker for vascular studies in Xenopus, we cloned the Xenopus laevis ortholog and analyzed its expression pattern during embryogenesis.
authors.affiliation: Martin-Luther-University Halle-Wittenberg, Institute for Physiological Chemistry, Halle (Saale), Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Neuhaus
authors.forename: Sanjeeva
authors.initials: S
authors.lastname: Metikala
authors.forename: Thomas
authors.initials: T
authors.lastname: Hollemann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015153:Blotting, Western; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000196:embryology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D055018:Hemangioblasts; D017403:In Situ Hybridization; D008648:Mesoderm / Q000196:embryology / Q000378:metabolism*; D008969:Molecular Sequence Data; D018919:Neovascularization, Physiologic / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014982:Xenopus laevis / Q000196:embryology / Q000378:metabolism*; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D012333:RNA, Messenger; D000099152:Cadherin 5