title: Effects of an iron-based fuel-borne catalyst and a diesel particle filter on exhaust toxicity in lung cells in vitro.
abstract: Metal-containing fuel additives catalyzing soot combustion in diesel particle filters are used in a widespread manner, and with the growing popularity of diesel vehicles, their application is expected to increase in the near future. Detailed investigation into how such additives affect exhaust toxicity is therefore necessary and has to be performed before epidemiological evidence points towards adverse effects of their application. The present study investigates how the addition of an iron-based fuel additive (Satacen®3, 40 ppm Fe) to low-sulfur diesel affects the in vitro cytotoxic, oxidative, (pro-)inflammatory, and mutagenic activity of the exhaust of a passenger car operated under constant, low-load conditions by exposing a three-dimensional model of the human airway epithelium to complete exhaust at the air-liquid interface. We could show that the use of the iron catalyst without and with filter technology has positive as well as negative effects on exhaust toxicity compared to exhaust with no additives: it decreases the oxidative and, compared to a non-catalyzed diesel particle filter, the mutagenic potential of diesel exhaust, but increases (pro-)inflammatory effects. The presence of a diesel particle filter also influences the impact of Satacen®3 on exhaust toxicity, and the proper choice of the filter type to be used is of importance with regards to exhaust toxicity. Figure ᅟ.
authors.affiliation: Adolphe Merkle Institute, University of Fribourg, Route de l'Ancienne Papeterie, P.O. Box 209, 1723, Fribourg, Switzerland.
authors.forename: Sandro
authors.initials: S
authors.lastname: Steiner
authors.forename: Jan
authors.initials: J
authors.lastname: Czerwinski
authors.forename: Pierre
authors.initials: P
authors.lastname: Comte
authors.forename: Norbert V
authors.initials: NV
authors.lastname: Heeb
authors.forename: Andreas
authors.initials: A
authors.lastname: Mayer
authors.forename: Alke
authors.initials: A
authors.lastname: Petri-Fink
authors.forename: Barbara
authors.initials: B
authors.lastname: Rothen-Rutishauser
mesh_terms: D061808:Air Filters; D002384:Catalysis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004781:Environmental Exposure / Q000009:adverse effects* / Q000032:analysis*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism; D007501:Iron / Q000737:chemistry*; D008168:Lung / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects; D013455:Sulfur / Q000737:chemistry; D001335:Vehicle Emissions / Q000032:analysis / Q000633:toxicity*
chemical_list: D009153:Mutagens; D001335:Vehicle Emissions; D013455:Sulfur; D007501:Iron