title: The Emerging Role of Reactive Oxygen Species Signaling during Lateral Root Development.
abstract: Overall root architecture is the combined result of primary and lateral root growth and is influenced by both intrinsic genetic programs and external signals. One of the main questions for root biologists is how plants control the number of lateral root primordia and their emergence through the main root. We recently identified S-phase kinase-associated protein2 (SKP2B) as a new early marker for lateral root development. Here, we took advantage of its specific expression pattern in Arabidopsis (Arabidopsis thaliana) in a cell-sorting and transcriptomic approach to generate a lateral root-specific cell sorting SKP2B data set that represents the endogenous genetic developmental program. We first validated this data set by showing that many of the identified genes have a function during root growth or lateral root development. Importantly, genes encoding peroxidases were highly represented in our data set. Thus, we next focused on this class of enzymes and showed, using genetic and chemical inhibitor studies, that peroxidase activity and reactive oxygen species signaling are specifically required during lateral root emergence but, intriguingly, not for primordium specification itself.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain (C.M., M.P.-B., I.C., J.C.d.P.);Universidad de Extremadura, Facultad de Ciencias, 06006 Badajoz, Spain (M.P.-B., I.C., P.C.);Department of Plant Systems Biology, Integrative Plant Biology Division, Flanders Interuniversity Institute for Biotechnology, B-9052 Ghent, Belgium (B.D.R., B.O.-L., G.V.I., T.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (B.D.R., G.V.I., T.B.); andUniversite Catholique de Louvain, Earth and Life Institute, B-1348 Louvain-la-Neuve, Belgium (B.O.-L., X.D.).
authors.forename: Concepción
authors.initials: C
authors.lastname: Manzano
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain (C.M., M.P.-B., I.C., J.C.d.P.);Universidad de Extremadura, Facultad de Ciencias, 06006 Badajoz, Spain (M.P.-B., I.C., P.C.);Department of Plant Systems Biology, Integrative Plant Biology Division, Flanders Interuniversity Institute for Biotechnology, B-9052 Ghent, Belgium (B.D.R., B.O.-L., G.V.I., T.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (B.D.R., G.V.I., T.B.); andUniversite Catholique de Louvain, Earth and Life Institute, B-1348 Louvain-la-Neuve, Belgium (B.O.-L., X.D.).
authors.forename: Mercedes
authors.initials: M
authors.lastname: Pallero-Baena
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain (C.M., M.P.-B., I.C., J.C.d.P.);Universidad de Extremadura, Facultad de Ciencias, 06006 Badajoz, Spain (M.P.-B., I.C., P.C.);Department of Plant Systems Biology, Integrative Plant Biology Division, Flanders Interuniversity Institute for Biotechnology, B-9052 Ghent, Belgium (B.D.R., B.O.-L., G.V.I., T.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (B.D.R., G.V.I., T.B.); andUniversite Catholique de Louvain, Earth and Life Institute, B-1348 Louvain-la-Neuve, Belgium (B.O.-L., X.D.).
authors.forename: Ilda
authors.initials: I
authors.lastname: Casimiro
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain (C.M., M.P.-B., I.C., J.C.d.P.);Universidad de Extremadura, Facultad de Ciencias, 06006 Badajoz, Spain (M.P.-B., I.C., P.C.);Department of Plant Systems Biology, Integrative Plant Biology Division, Flanders Interuniversity Institute for Biotechnology, B-9052 Ghent, Belgium (B.D.R., B.O.-L., G.V.I., T.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (B.D.R., G.V.I., T.B.); andUniversite Catholique de Louvain, Earth and Life Institute, B-1348 Louvain-la-Neuve, Belgium (B.O.-L., X.D.).
authors.forename: Bert
authors.initials: B
authors.lastname: De Rybel
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain (C.M., M.P.-B., I.C., J.C.d.P.);Universidad de Extremadura, Facultad de Ciencias, 06006 Badajoz, Spain (M.P.-B., I.C., P.C.);Department of Plant Systems Biology, Integrative Plant Biology Division, Flanders Interuniversity Institute for Biotechnology, B-9052 Ghent, Belgium (B.D.R., B.O.-L., G.V.I., T.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (B.D.R., G.V.I., T.B.); andUniversite Catholique de Louvain, Earth and Life Institute, B-1348 Louvain-la-Neuve, Belgium (B.O.-L., X.D.).
authors.forename: Beata
authors.initials: B
authors.lastname: Orman-Ligeza
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain (C.M., M.P.-B., I.C., J.C.d.P.);Universidad de Extremadura, Facultad de Ciencias, 06006 Badajoz, Spain (M.P.-B., I.C., P.C.);Department of Plant Systems Biology, Integrative Plant Biology Division, Flanders Interuniversity Institute for Biotechnology, B-9052 Ghent, Belgium (B.D.R., B.O.-L., G.V.I., T.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (B.D.R., G.V.I., T.B.); andUniversite Catholique de Louvain, Earth and Life Institute, B-1348 Louvain-la-Neuve, Belgium (B.O.-L., X.D.).
authors.forename: Gert
authors.initials: G
authors.lastname: Van Isterdael
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain (C.M., M.P.-B., I.C., J.C.d.P.);Universidad de Extremadura, Facultad de Ciencias, 06006 Badajoz, Spain (M.P.-B., I.C., P.C.);Department of Plant Systems Biology, Integrative Plant Biology Division, Flanders Interuniversity Institute for Biotechnology, B-9052 Ghent, Belgium (B.D.R., B.O.-L., G.V.I., T.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (B.D.R., G.V.I., T.B.); andUniversite Catholique de Louvain, Earth and Life Institute, B-1348 Louvain-la-Neuve, Belgium (B.O.-L., X.D.).
authors.forename: Tom
authors.initials: T
authors.lastname: Beeckman
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain (C.M., M.P.-B., I.C., J.C.d.P.);Universidad de Extremadura, Facultad de Ciencias, 06006 Badajoz, Spain (M.P.-B., I.C., P.C.);Department of Plant Systems Biology, Integrative Plant Biology Division, Flanders Interuniversity Institute for Biotechnology, B-9052 Ghent, Belgium (B.D.R., B.O.-L., G.V.I., T.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (B.D.R., G.V.I., T.B.); andUniversite Catholique de Louvain, Earth and Life Institute, B-1348 Louvain-la-Neuve, Belgium (B.O.-L., X.D.).
authors.forename: Xavier
authors.initials: X
authors.lastname: Draye
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain (C.M., M.P.-B., I.C., J.C.d.P.);Universidad de Extremadura, Facultad de Ciencias, 06006 Badajoz, Spain (M.P.-B., I.C., P.C.);Department of Plant Systems Biology, Integrative Plant Biology Division, Flanders Interuniversity Institute for Biotechnology, B-9052 Ghent, Belgium (B.D.R., B.O.-L., G.V.I., T.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (B.D.R., G.V.I., T.B.); andUniversite Catholique de Louvain, Earth and Life Institute, B-1348 Louvain-la-Neuve, Belgium (B.O.-L., X.D.).
authors.forename: Pedro
authors.initials: P
authors.lastname: Casero
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain (C.M., M.P.-B., I.C., J.C.d.P.);Universidad de Extremadura, Facultad de Ciencias, 06006 Badajoz, Spain (M.P.-B., I.C., P.C.);Department of Plant Systems Biology, Integrative Plant Biology Division, Flanders Interuniversity Institute for Biotechnology, B-9052 Ghent, Belgium (B.D.R., B.O.-L., G.V.I., T.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (B.D.R., G.V.I., T.B.); andUniversite Catholique de Louvain, Earth and Life Institute, B-1348 Louvain-la-Neuve, Belgium (B.O.-L., X.D.) pozo@inia.es.
authors.forename: Juan C
authors.identifier: http://orcid.org/0000-0002-4113-457X
authors.initials: JC
authors.lastname: Del Pozo