title: Identiﬁcation of multiple constituents in Chinese medicinal prescription Shensong Yangxin capsule by ultra-fast liquid chromatography combined with quadrupole time-of-flight mass spectrometry.
abstract: A practical method using ultra-fast liquid chromatography in tandem with quadrupole time-of-ﬂight mass spectrometry combined with dynamic background subtraction technology was developed for the rapid separation and identiﬁcation of the complicated constituents in the Shensong Yangxin capsule (SSYX). The chromatographic separation was performed on a C18 column (2.1 × 100 mm, 2.6 μm) with a gradient elution program using methanol and 0.1% formic acid aqueous solution as the mobile phase at a ﬂow rate of 0.4 mL min(-1). Accurate mass measurements of the molecular ions in the full scan and the characteristic fragment ions triggered by information-dependent acquisition provided reliable identiﬁcation criteria. Thus, 99 compounds, including saponins, phenolic acids, tanshinones, lignans, terpenoids, alkaloids and flavonoids, were unambiguously or tentatively identiﬁed in 40 min by comparing their retention times and accurate mass measurements for each molecular ion and its subsequent fragment ions with those of authentic standards or literature data. Simultaneously, all the compounds were further assigned to the individual raw materials. In conclusion, these results will provide a basis for quality control and further study of SSYX, and the proposed technique based on high-resolution mass spectrometry would be expected to be adaptable to the analysis of complicated constituents in various complex matrices.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, 38 Xueyuan Road, Beijing 100191, China Shijiazhuang Yiling Pharmaceutical Co., Ltd, Shijiazhuang 050035, China.
authors.forename: Minyan
authors.initials: M
authors.lastname: Liu
authors.affiliation: Shijiazhuang Yiling Pharmaceutical Co., Ltd, Shijiazhuang 050035, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Shijiazhuang Yiling Pharmaceutical Co., Ltd, Shijiazhuang 050035, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shanghai AB Sciex Analytical Instrument Trading Co., Ltd, Shanghai 200040, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Shijiazhuang Yiling Pharmaceutical Co., Ltd, Shijiazhuang 050035, China.
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.affiliation: Shijiazhuang Yiling Pharmaceutical Co., Ltd, Shijiazhuang 050035, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, 38 Xueyuan Road, Beijing 100191, China pengfeitu@vip.163.com.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Tu
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D062385:Hydroxybenzoates / Q000032:analysis / Q000737:chemistry; D012503:Saponins / Q000032:analysis / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D062385:Hydroxybenzoates; D012503:Saponins; C530959:shensongyangxin; C017616:phenolic acid