title: Recessive mutation identifies auxin-repressed protein ARP1, which regulates growth and disease resistance in tobacco.
abstract: To study the molecular mechanism that underpins crosstalk between plant growth and disease resistance, we performed a mutant screening on tobacco and created a recessive mutation that caused the phenotype of growth enhancement and resistance impairment (geri1). In the geri1 mutant, growth enhancement accompanies promoted expression of growth-promoting genes, whereas repressed expression of defense response genes is consistent with impaired resistance to diseases caused by viral, bacterial, and oomycete pathogens. The geri1 allele identifies a single genetic locus hypothetically containing the tagged GERI1 gene. The isolated GERI1 gene was predicted to encode auxin-repressed protein ARP1, which was determined to be 13.5 kDa in size. The ARP1/GERI1 gene was further characterized as a repressor of plant growth and an activator of disease resistance based on genetic complementation, gene silencing, and overexpression analyses. ARP1/GERI1 resembles pathogen-associated molecular patterns and is required for them to repress plant growth and activate plant immunity responses. ARP1/GERI1 represses growth by inhibiting the expression of AUXIN RESPONSE FACTOR gene ARF8, and ARP1/GERI1 recruits the NPR1 gene, which is essential for the salicylic-acid-mediated defense, to coregulate disease resistance. In conclusion, ARP1/GERI1 is an integral regulator for crosstalk between growth and disease resistance in the plant.
authors.forename: Yanying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Ge
authors.forename: Manyu
authors.initials: M
authors.lastname: Xu
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhu
authors.forename: Tingquan
authors.initials: T
authors.lastname: Wu
authors.forename: An
authors.initials: A
authors.lastname: Guo
authors.forename: Junyi
authors.initials: J
authors.lastname: Xie
authors.forename: Hansong
authors.initials: H
authors.lastname: Dong
mesh_terms: D000595:Amino Acid Sequence; D002686:Chitin; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000276:immunology / Q000502:physiology; D020022:Genetic Predisposition to Disease; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D002686:Chitin