title: In vivo evaluation of the dopaminergic neurotransmission system using [123I]FP-CIT SPECT in 6-OHDA lesioned rats.
abstract: The 6-hydroxydopamine (6-OHDA) rodent model of Parkinson's disease (PD) has been used to evaluate the nigrostriatal pathway. The aim of this work was to explore the relationship between the degree of 6-OHDA-induced dopaminergic degeneration and [(123)I]FP-CIT binding using single photon emission computed tomography (SPECT). Fourteen rats received a 6-OHDA injection (4 or 8 µg) into the left medial forebrain bundle. After 3 weeks, magnetic resonance imaging and scans with a small-animal SPECT system were performed. Finally, the nigrostriatal lesion was assessed by immunohistochemical analysis. Immunohistochemical analysis confirmed two levels of dopaminergic degeneration. Lesions induced by 6-OHDA diminished the ipsilateral [(123)I]FP-CIT binding by 61 and 76%, respectively. The decrease in tracer uptake between control and lesioned animals was statistically significant, as was the difference between the two 6-OHDA lesioned groups. Results concluded that [(123)I]FP-CIT SPECT is a useful technique to discriminate the degree of dopaminergic degeneration in a rat model of PD.
authors.affiliation: Unitat de Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina, Barcelona, Spain.
authors.forename: Aida
authors.initials: A
authors.lastname: Niñerola-Baizán
authors.forename: Santiago
authors.initials: S
authors.lastname: Rojas
authors.forename: Mercè
authors.initials: M
authors.lastname: Bonastre
authors.forename: Raúl
authors.initials: R
authors.lastname: Tudela
authors.forename: Francisco
authors.initials: F
authors.lastname: Lomeña
authors.forename: Javier
authors.initials: J
authors.lastname: Pavía
authors.forename: Concepció
authors.initials: C
authors.lastname: Marin
authors.forename: Domènec
authors.initials: D
authors.lastname: Ros
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000000981:diagnostic imaging / Q000473:pathology; D059290:Dopaminergic Neurons / Q000000981:diagnostic imaging / Q000473:pathology; D006801:Humans; D008279:Magnetic Resonance Imaging; D016627:Oxidopamine; D010300:Parkinson Disease / Q000000981:diagnostic imaging* / Q000473:pathology; D011859:Radiography; D051381:Rats; D009435:Synaptic Transmission; D015899:Tomography, Emission-Computed, Single-Photon; D014326:Tropanes
chemical_list: D014326:Tropanes; C087552:2-carbomethoxy-8-(3-fluoropropyl)-3-(4-iodophenyl)tropane; D016627:Oxidopamine
keywords: 6-OHDA; Parkinson's disease; SPECT; [123I]FP-CIT; molecular imaging; rat