title: Biosynthesis of trans-4-hydroxyproline by recombinant strains of Corynebacterium glutamicum and Escherichia coli.
abstract: BACKGROUND
  Trans-4-hydroxy-L-proline (trans-Hyp), one of the hydroxyproline (Hyp) isomers, is a useful chiral building block in the production of many pharmaceuticals. Although there are some natural biosynthetic pathways of trans-Hyp existing in microorganisms, the yield is still too low to be scaled up for industrial applications. Until now the production of trans-Hyp is mainly from the acid hydrolysis of collagen. Due to the increasing environmental concerns on those severe chemical processes and complicated downstream separation, it is essential to explore some environment-friendly processes such as constructing new recombinant strains to develop efficient process for trans-Hyp production.
  
  
  RESULTS
  In this study, the genes of trans-proline 4-hydroxylase (trans-P4H) from diverse resources were cloned and expressed in Corynebacterium glutamicum and Escherichia coli, respectively. The trans-Hyp production by these recombinant strains was investigated. The results showed that all the genes from different resources had been expressed actively. Both the recombinant C. glutamicum and E. coli strains could produce trans-Hyp in the absence of proline and 2-oxoglutarate.
  
  
  CONCLUSIONS
  The whole cell microbial systems for trans-Hyp production have been successfully constructed by introducing trans-P4H into C. glutamicum and E. coli. Although the highest yield was obtained in recombinant E. coli, using recombinant C. glutamicum strains to produce trans-Hyp was a new attempt.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Yi
authors.forename: Huakai
authors.initials: H
authors.lastname: Sheng
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. lizm@ecust.edu.cn.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Li
authors.forename: Qin
authors.initials: Q
authors.lastname: Ye
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000201:enzymology / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D006909:Hydroxyproline / Q000096:biosynthesis* / Q000737:chemistry; D007536:Isomerism; D064828:Prolyl Hydroxylases / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D064828:Prolyl Hydroxylases; D006909:Hydroxyproline