title: An historical perspective on how advances in microscopic imaging contributed to understanding the Leishmania Spp. and Trypanosoma cruzi host-parasite relationship.
abstract: The literature has identified complex aspects of intracellular host-parasite relationships, which require systematic, nonreductionist approaches and spatial/temporal information. Increasing and integrating temporal and spatial dimensions in host cell imaging have contributed to elucidating several conceptual gaps in the biology of intracellular parasites. To access and investigate complex and emergent dynamic events, it is mandatory to follow them in the context of living cells and organs, constructing scientific images with integrated high quality spatiotemporal data. This review discusses examples of how advances in microscopy have challenged established conceptual models of the intracellular life cycles of Leishmania spp. and Trypanosoma cruzi protozoan parasites.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu 862, 6th Floor, 04023-062 São Paulo, SP, Brazil.
authors.forename: P T V
authors.initials: PT
authors.lastname: Florentino
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu 862, 6th Floor, 04023-062 São Paulo, SP, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Real
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu 862, 6th Floor, 04023-062 São Paulo, SP, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Bonfim-Melo
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu 862, 6th Floor, 04023-062 São Paulo, SP, Brazil.
authors.forename: C M
authors.initials: CM
authors.lastname: Orikaza
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu 862, 6th Floor, 04023-062 São Paulo, SP, Brazil.
authors.forename: E R
authors.initials: ER
authors.lastname: Ferreira
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu 862, 6th Floor, 04023-062 São Paulo, SP, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Pessoa
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu 862, 6th Floor, 04023-062 São Paulo, SP, Brazil.
authors.forename: B R
authors.initials: BR
authors.lastname: Lima
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu 862, 6th Floor, 04023-062 São Paulo, SP, Brazil.
authors.forename: G R S
authors.initials: GR
authors.lastname: Sasso
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu 862, 6th Floor, 04023-062 São Paulo, SP, Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Mortara
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000473:pathology*; D006790:Host-Parasite Interactions / Q000502:physiology*; D006801:Humans; D007891:Leishmania / Q000502:physiology*; D007896:Leishmaniasis / Q000473:pathology*; D008853:Microscopy; D014349:Trypanosoma cruzi / Q000502:physiology*