title: Up-regulation of DNAM-1 and NKp30, associated with improvement of NK cells activation after long-term culture of mononuclear cells from patients with ovarian neoplasia.
abstract: This study aimed at evaluating the functional activation and activating receptors expression on resting, short- and long-term NK and NK-like T cells from blood of ovarian neoplasia patients. Blood from patients with adnexal benign alterations (n = 10) and ovarian cancer (grade I-IV n = 14) were collected after signed consent. Effector cells activation was evaluated by the expression of the CD107a molecule. Short-term culture was conducted overnight with IL-2 and long-term culture for 21 days, by a method designed to expand CD56(+) lymphocytes. Short-term culture significantly increased NK cells activation compared to resting NK cells (p<0.05), however, the long-term procedure supported an even higher increase (p<0.001). Resting NK-like T cells showed poor activation, which was not altered by the culture procedures. The long-term culture effectively increased the expression of the activating receptors on NK and NK-like T cells, either by increasing the number of cells expressing a given receptor and/or by up-regulating their expression intensity. As a conclusion, the long-term culture system employed, resulted in a high number of functional NK cells. The culture system was particularly efficient on the up-regulation of NKp30 and DNAM-1 receptors on NK cells.
authors.affiliation: Hospital da Mulher Professor Doutor José Aristodemo Pinotti - Centro de Atenção Integral à Saúde da Mulher, University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Rodrigo Fernandes
authors.initials: RF
authors.lastname: da Silva
authors.affiliation: Hospital da Mulher Professor Doutor José Aristodemo Pinotti - Centro de Atenção Integral à Saúde da Mulher, University of Campinas (UNICAMP), Campinas, Brazil; Departamento de Tocoginecologia, Faculdade de Ciências Médicas, University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Petta
authors.affiliation: Hospital da Mulher Professor Doutor José Aristodemo Pinotti - Centro de Atenção Integral à Saúde da Mulher, University of Campinas (UNICAMP), Campinas, Brazil; Departamento de Tocoginecologia, Faculdade de Ciências Médicas, University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Sophie Françoise
authors.initials: SF
authors.lastname: Derchain
authors.affiliation: Cell and Gene Therapy, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden.
authors.forename: Evren
authors.initials: E
authors.lastname: Alici
authors.affiliation: Hospital da Mulher Professor Doutor José Aristodemo Pinotti - Centro de Atenção Integral à Saúde da Mulher, University of Campinas (UNICAMP), Campinas, Brazil. Electronic address: fernando@caism.unicamp.br.
authors.forename: Fernando
authors.initials: F
authors.lastname: Guimarães
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D018284:Cystadenocarcinoma, Serous / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation; D052118:Lysosomal-Associated Membrane Protein 1 / Q000235:genetics / Q000276:immunology; D008875:Middle Aged; D055628:Natural Cytotoxicity Triggering Receptor 3 / Q000235:genetics* / Q000276:immunology; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D010053:Ovary / Q000276:immunology / Q000473:pathology; D061251:Primary Cell Culture; D015398:Signal Transduction; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D000098862:T Lineage-Specific Activation Antigen 1
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; D007376:Interleukin-2; D052118:Lysosomal-Associated Membrane Protein 1; C431710:NCR3 protein, human; D055628:Natural Cytotoxicity Triggering Receptor 3
keywords: CD107a; Degranulation; NCRs (natural cytotoxic receptors); NK-like T lymphocytes; Regulatory T cells