title: Decellularized kidney scaffold-mediated renal regeneration.
abstract: Renal regeneration approaches offer great potential for the treatment of chronic kidney disease, but their availability remains limited by the clinical challenges they pose. In the present study, we used continuous detergent perfusion to generate decellularized (DC) rat kidney scaffolds. The scaffolds retained intact vascular trees and overall architecture, along with significant concentrations of various cytokines, but lost all cellular components. To evaluate its potential in renal function recovery, DC scaffold tissue was grafted onto partially nephrectomized rat kidneys. An increase of renal size was found, and regenerated renal parenchyma cells were observed in the repair area containing the grafted scaffold. In addition, the number of nestin-positive renal progenitor cells was markedly higher in scaffold-grafted kidneys compared to controls. Moreover, radionuclide scan analysis showed significant recovery of renal functions at 6 weeks post-implantation. Our results provide further evidence to show that DC kidney scaffolds could be used to promote renal recovery in the treatment of chronic kidney disease.
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou 325035, China; Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Yu
authors.affiliation: School of the First Clinical Medical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Y K
authors.initials: YK
authors.lastname: Shao
authors.affiliation: Institute of Neuroscience, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Ding
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: K Z
authors.initials: KZ
authors.lastname: Lin
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou 325035, China; Department of Radiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 32000, China.
authors.forename: B
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Nuclear Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 32000, China.
authors.forename: H Z
authors.initials: HZ
authors.lastname: Zhang
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou 325035, China; Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: L N
authors.initials: LN
authors.lastname: Zhao
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Z B
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou 325035, China; Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: J S
authors.initials: JS
authors.lastname: Zhang
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou 325035, China; Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: M L
authors.initials: ML
authors.lastname: Tang
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou 325035, China; Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou 325035, China; Institute of Neuroscience, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: tibetcn@email.com.
authors.forename: J
authors.initials: J
authors.lastname: Mei
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000737:chemistry; D007668:Kidney / Q000378:metabolism*; D008297:Male; D046529:Microscopy, Electron, Transmission; D009392:Nephrectomy; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D051436:Renal Insufficiency, Chronic / Q000628:therapy; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013234:Stem Cells / Q000378:metabolism; D013501:Surface-Active Agents / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D016207:Cytokines; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate
keywords: Partial nephrectomy; Renal regeneration; Scaffold; Stem/progenitor cells