title: Baclofen and 2-hydroxysaclofen modify acute hypolocomotive and antinociceptive effects of nicotine.
abstract: The aim of the present study was to evaluate the possible involvement of GABAB receptors in nicotine-induced hypolocomotion and antinociceptive effects in mice. Animals were exposed to nicotine only once. Acute nicotine hydrogen tartrate salt (3mg/kg; subcutaneous, s.c.) administration induced hypolocomotion and antinociceptive responses in the tail-immersion and the hot-plate tests. The effects of pretreatment with either the GABAB receptor agonist baclofen (1, 2 and 3mg/kg; intraperitoneal, i.p.) or GABAB receptor antagonist 2-hydroxysaclofen (0.25, 0.5 and 1mg/kg; i.p.) were evaluated on these behavioral nicotine responses. The GABAB receptor agonist, baclofen (3mg/kg, i.p.) abolished nicotine-induced antinociceptive effects in the tail-immersion and the hot-plate tests, but did not modify nicotine-induced hypolocomotion. In addition, the GABAB receptor antagonist, 2-hydroxysaclofen (1mg/kg, i.p.) increased nicotine-induced antinociceptive effects in the tail-immersion and the hot-plate tests, and abolished nicotine-induced hypolocomotion. The present results shed light that the GABAB receptor has an important role in mediating specific acute nicotine responses such as hypolocomotion and antinociception in mice.
authors.affiliation: Instituto de Investigaciones Farmacológicas (CONICET). Junín 956 5° Piso, Buenos Aires C1113AAD, Argentina.
authors.forename: Andrés P
authors.initials: AP
authors.lastname: Varani
authors.affiliation: Laboratori de Neurofarmacologia, Facultat de Ciències de la Salut i de la Vida, Universitat Pompeu Fabra, C/Dr. Aiguader, 88, 08003 Barcelona, Spain.
authors.forename: Ester
authors.initials: E
authors.lastname: Aso
authors.affiliation: Laboratori de Neurofarmacologia, Facultat de Ciències de la Salut i de la Vida, Universitat Pompeu Fabra, C/Dr. Aiguader, 88, 08003 Barcelona, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Maldonado
authors.affiliation: Instituto de Investigaciones Farmacológicas (CONICET). Junín 956 5° Piso, Buenos Aires C1113AAD, Argentina; Cátedra de Farmacología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 5° Piso, Buenos Aires C1113AAD, Argentina. Electronic address: gbalerio@ffyb.uba.ar.
authors.forename: Graciela N
authors.initials: GN
authors.lastname: Balerio
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D001418:Baclofen / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004347:Drug Interactions; D058786:GABA-B Receptor Agonists / Q000494:pharmacology*; D058788:GABA-B Receptor Antagonists / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects*; D009538:Nicotine / Q000494:pharmacology*
chemical_list: D000700:Analgesics; D058786:GABA-B Receptor Agonists; D058788:GABA-B Receptor Antagonists; D009538:Nicotine; D001418:Baclofen; C057491:2-hydroxysaclofen
keywords: 2-hydroxysaclofen; Baclofen; GABA(B) receptors; Locomotion; Nicotine; Nociception