title: Functionalized polyglycerol amine nanogels as nanocarriers for DNA.
abstract: Polyglycerol based nanogels (nPG) can function as cellular delivery systems. These nPGs are synthesized with different amine densities (nPG amines) by acid-catalyzed epoxide-opening polymerization using a mini-emulsion approach and surface modification. All the synthesized nanogels are characterized by NMR, dynamic light scattering, and ζ-potential, showing slightly positive surface charge and a homogeneous size of ≈100 nm. The use of these systems for delivery applications is demonstrated with regard to polyplex formation, cytotoxicity, and cellular uptake studies. It is depicted that the CE50 value of the high loaded nPG amines is eight times higher than the low loaded ones. The influence of the amine loading percentage on the nanogel and the effects of polyvalency in these architecture is discussed.
authors.affiliation: Institute of Chemistry and Biochemistry - Organic Chemistry, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Hellmund
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhou
authors.forename: Olga
authors.initials: O
authors.lastname: Samsonova
authors.forename: Pia
authors.initials: P
authors.lastname: Welker
authors.forename: Thomas
authors.initials: T
authors.lastname: Kissel
authors.forename: Rainer
authors.initials: R
authors.lastname: Haag
mesh_terms: D000588:Amines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D004247:DNA / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D005990:Glycerol / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D012542:Scattering, Radiation
chemical_list: D000588:Amines; D004337:Drug Carriers; D011108:Polymers; C043941:polyglycerol; D004247:DNA; D005990:Glycerol
keywords: DNA delivery; cationic architectures; cellular uptake; polyglycerol based nanogels