title: Dynamic clonal analysis based on chronic in vivo imaging allows multiscale quantification of growth in the Drosophila wing disc.
abstract: In the course of morphogenesis, tissues change shape and grow. How this is orchestrated is largely unknown, partly owing to the lack of experimental methods to visualize and quantify growth. Here, we describe a novel experimental approach to investigate the growth of tissues in vivo on a time-scale of days, as employed to study the Drosophila larval imaginal wing disc, the precursor of the adult wing. We developed a protocol to image wing discs at regular intervals in living anesthetized larvae so as to follow the growth of the tissue over extended periods of time. This approach can be used to image cells at high resolution in vivo. At intermediate scale, we tracked the increase in cell number within clones as well as the changes in clone area and shape. At scales extending to the tissue level, clones can be used as landmarks for measuring strain, as a proxy for growth. We developed general computational tools to extract strain maps from clonal shapes and landmark displacements in individual tissues, and to combine multiple datasets into a mean strain. In the disc, we use these to compare properties of growth at the scale of clones (a few cells) and at larger regional scales.
authors.affiliation: Kavli Institute for Theoretical Physics, University of California, Santa Barbara, CA 93106, USA.
authors.forename: Idse
authors.initials: I
authors.lastname: Heemskerk
authors.affiliation: Aix Marseille Université, CNRS, IBDML UMR7288, case 907, Marseille 13009, France thomas.lecuit@univ-amu.fr loic.le-goff@univ-amu.fr.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lecuit
authors.affiliation: Aix Marseille Université, CNRS, IBDML UMR7288, case 907, Marseille 13009, France thomas.lecuit@univ-amu.fr loic.le-goff@univ-amu.fr.
authors.forename: Loïc
authors.initials: L
authors.lastname: LeGoff
mesh_terms: D000818:Animals; D020169:Caspases / Q000378:metabolism; D019295:Computational Biology; D004330:Drosophila; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D060227:Imaginal Discs / Q000196:embryology* / Q000254:growth & development; D008856:Microscopy, Fluorescence / Q000379:methods*; D008962:Models, Theoretical; D013314:Stress, Mechanical; D014921:Wings, Animal / Q000196:embryology* / Q000254:growth & development
chemical_list: D049452:Green Fluorescent Proteins; D020169:Caspases
keywords: Drosophila wing; Growth; Image analysis; In vivo imaging; Mathematical analysis; Morphogenesis