title: Immunohistochemical localization of steroid receptor coactivators in chondrosarcoma: an in vivo tissue microarray study.
abstract: Chondrosarcoma is the second most common type of primary bone malignancy following up osteosarcoma, characterized by resistance to conventional chemotherapeutic agents and radiation regimens. The p160 family members steroid receptor coactivator-1 and -3 (SRC-1 and SRC-3) have been implied in the regulation of cancer growth, migration, invasion, metastasis and chemotherapeutic resistance; but we still lack detailed information about the levels of SRCs in chondrosarcoma. In this study, expression of SRC-1 and SRC-3 in chondrosarcoma was examined by immunohistochemistry with tissue microarrays; the four score system (0, 1, 2 and 3) was used to evaluate the staining. The results showed that there were no gender-, site- or age-differences regarding the expression of SRC-1 or SRC-3 (p>0.05); organ (bone or cartilage) -differences were only detected for SRC-1 but not SRC-3 (p<0.05). Significant higher levels of SRC-1 and SRC-3 were detected in MDC and PDC when compared to WDC. Our study clearly demonstrated differentiation-dependant expression of SRC-1 and SRC-3 in chondrosarcoma, may be novel targets for the prognosis and/or treatment of chondrosarcoma, would have opened a new avenue and established foundation for studying chondrosarcoma.
authors.affiliation: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopaedics, Southwest Hospital, Third Military Medical University, Chongqing 400038, China; Department of Orthopedic Surgery, Affiliated Hospital of Bengbu Medical College, Bengbu 233030, Anhui Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopaedics, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jingshu
authors.initials: J
authors.lastname: Fu
authors.affiliation: Department of Neurobiology, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Bian
authors.affiliation: Department of Neurobiology, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jiqiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopaedics, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. Electronic address: xiezhao54981@163.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Xie
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D002813:Chondrosarcoma / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D056920:Nuclear Receptor Coactivator 1 / Q000378:metabolism*; D056921:Nuclear Receptor Coactivator 3 / Q000378:metabolism*; D011379:Prognosis; D012737:Sex Factors; D046888:Tissue Array Analysis; D055815:Young Adult
chemical_list: D056920:Nuclear Receptor Coactivator 1; D056921:Nuclear Receptor Coactivator 3
keywords: Chondrosarcoma; Immunohistochemistry; SRC-1; SRC-3; Tissue microarray