title: Development and application of quantitative detection method for viral hemorrhagic septicemia virus (VHSV) genogroup IVa.
abstract: Viral hemorrhagic septicemia virus (VHSV) is a problematic pathogen in olive flounder (Paralichthys olivaceus) aquaculture farms in Korea. Thus, it is necessary to develop a rapid and accurate diagnostic method to detect this virus. We developed a quantitative RT-PCR (qRT-PCR) method based on the nucleocapsid (N) gene sequence of Korean VHSV isolate (Genogroup IVa). The slope and R² values of the primer set developed in this study were -0.2928 (96% efficiency) and 0.9979, respectively. Its comparison with viral infectivity calculated by traditional quantifying method (TCID₅₀) showed a similar pattern of kinetic changes in vitro and in vivo. The qRT-PCR method reduced detection time compared to that of TCID₅₀, making it a very useful tool for VHSV diagnosis.
authors.affiliation: Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Korea. jongoh.kim77@gmail.com.
authors.forename: Jong-Oh
authors.initials: JO
authors.lastname: Kim
authors.affiliation: Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Korea. wisky@chonnam.ac.kr.
authors.forename: Wi-Sik
authors.initials: WS
authors.lastname: Kim
authors.affiliation: Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Korea. kimman2309@naver.com.
authors.forename: Si-Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Aquatic Life Disease Control Division, National Fisheries Research and Development Institute, Busan 619-902, Korea. hjhan77@korea.kr.
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Han
authors.affiliation: Aquatic Life Disease Control Division, National Fisheries Research and Development Institute, Busan 619-902, Korea. jwkim123@korea.kr.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Aquatic Life Disease Control Division, National Fisheries Research and Development Institute, Busan 619-902, Korea. mapark@korea.kr.
authors.forename: Myoung Ae
authors.initials: MA
authors.lastname: Park
authors.affiliation: Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Korea. ohmj@chonnam.ac.kr.
authors.forename: Myung-Joo
authors.initials: MJ
authors.lastname: Oh
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000175:diagnosis* / Q000821:virology*; D005412:Flatfishes / Q000821:virology*; D005838:Genotype; D007723:Korea; D029141:Novirhabdovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019251:Nucleocapsid / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D013997:Time Factors; D019562:Viral Load / Q000379:methods*