title: Non-enzymatic lipid oxidation products in biological systems: assessment of the metabolites from polyunsaturated fatty acids.
abstract: Metabolites of non-enzymatic lipid peroxidation of polyunsaturated fatty acids notably omega-3 and omega-6 fatty acids have become important biomarkers of lipid products. Especially the arachidonic acid-derived F2-isoprostanes are the classic in vivo biomarker for oxidative stress in biological systems. In recent years other isoprostanes from eicosapentaenoic, docosahexaenoic, adrenic and α-linolenic acids have been evaluated, namely F3-isoprostanes, F4-neuroprostanes, F2-dihomo-isoprostanes and F1-phytoprostanes, respectively. These have been gaining interest as complementary specific biomarkers in human diseases. Refined extraction methods, robust analysis and elucidation of chemical structures have improved the sensitivity of detection in biological tissues and fluids. Previously the main reliable instrumentation for measurement was gas chromatography-mass spectrometry (GC-MS), but now the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immunological techniques is gaining much attention. In this review, the types of prostanoids generated from non-enzymatic lipid peroxidation of some important omega-3 and omega-6 fatty acids and biological samples that have been determined by GC-MS and LC-MS/MS are discussed.
authors.affiliation: Institut des Biomolécules Max Mousseron IBMM, UMR 5247 CNRS/Université Montpellier 1/Université Montpellier 2, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Vigor
authors.affiliation: Plateau de lipidomique, Bio-Medical Federative Research Institute of Toulouse, INSERM, Plateforme MetaToul, Toulouse, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Bertrand-Michel
authors.affiliation: Institut des Biomolécules Max Mousseron IBMM, UMR 5247 CNRS/Université Montpellier 1/Université Montpellier 2, France.
authors.forename: Edith
authors.initials: E
authors.lastname: Pinot
authors.affiliation: Institut des Biomolécules Max Mousseron IBMM, UMR 5247 CNRS/Université Montpellier 1/Université Montpellier 2, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Oger
authors.affiliation: Institut des Biomolécules Max Mousseron IBMM, UMR 5247 CNRS/Université Montpellier 1/Université Montpellier 2, France.
authors.forename: Joseph
authors.initials: J
authors.lastname: Vercauteren
authors.affiliation: Plateau de lipidomique, Bio-Medical Federative Research Institute of Toulouse, INSERM, Plateforme MetaToul, Toulouse, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Le Faouder
authors.affiliation: Institut des Biomolécules Max Mousseron IBMM, UMR 5247 CNRS/Université Montpellier 1/Université Montpellier 2, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Galano
authors.affiliation: The University of Hong Kong, School of Biological Sciences, Hong Kong SAR, China. Electronic address: jettylee@hku.hk.
authors.forename: Jetty Chung-Yung
authors.initials: JC
authors.lastname: Lee
authors.affiliation: Institut des Biomolécules Max Mousseron IBMM, UMR 5247 CNRS/Université Montpellier 1/Université Montpellier 2, France. Electronic address: Thierry.Durand@univ-montp1.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Durand
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005231:Fatty Acids, Unsaturated / Q000032:analysis* / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D028421:Isoprostanes / Q000032:analysis / Q000378:metabolism; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005231:Fatty Acids, Unsaturated; D028421:Isoprostanes
keywords: Fatty acid; GC–MS; Isoprostanes; LC–MS/MS; Lipid peroxidation; Oxidative stress