title: Dried saliva spot as a sampling technique for saliva samples.
abstract: For the first time, dried saliva spot (DSS) was used as a sampling technique for saliva samples. In the DSS technique 50 μL of saliva was collected on filter paper and the saliva was then extracted with an organic solvent. The local anesthetic lidocaine was used as a model compound, which was determined in the DSS using liquid chromatography and mass spectrometry. The results obtained for the determination of lidocaine in saliva using DSS were compared with those from a previous study using a microextraction by packed sorbent syringe as the sampling method for saliva. This study shows that DSS can be used for the analysis of saliva samples. The method is promising and very easy in terms of sampling and extraction procedures. The results from this study are in good agreement with those from our previous work on the determination of lidocaine in saliva. DSS can open a new dimension in the saliva handling process in terms of sampling, storing and transport.
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, M60 1QD, UK.
authors.forename: Abbi
authors.initials: A
authors.lastname: Abdel-Rehim
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdel-Rehim
mesh_terms: D000779:Anesthetics, Local / Q000032:analysis*; D002853:Chromatography, Liquid; D006801:Humans; D008012:Lidocaine / Q000032:analysis*; D013058:Mass Spectrometry; D012463:Saliva / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D000779:Anesthetics, Local; D008012:Lidocaine
keywords: LC/MS/MS; dried saliva spot; lidocaine; saliva