title: Exogenous normal lymph alleviates lipopolysaccharide-induced acute lung injury through lessening the adhesion molecules.
abstract: OBJECTIVE
  To evaluate the role of exogenous normal lymph (ENL) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats.
  
  
  METHODS
  ALI was induced by the jugular vein injection of LPS (iv, 15 mg/kg) in rats of the LPS and LPS+ENL groups within 15 min, then, ENL without cell components (5 ml/kg) was infused at the speed of 0.5 ml per minute in the LPS+ENL group, the same amount of saline was administered in the LPS group. The rats in the sham group received the same surgical procedure and saline. The histomorphology and the levels of P-selectin, intercellular adhesion molecule-1 (ICAM-1), myeloperoxidase (MPO) in pulmonary tissue were assessed.
  
  
  RESULTS
  LPS induced pulmonary injury as well as increased the wet/dry weight ratio (W/D) and the levels of P-selectin, ICAM-1, and MPO in pulmonary tissues. These deleterious effects of LPS were significantly ameliorated by ENL treatment.
  
  
  CONCLUSIONS
  Exogenous normal lymph could markedly alleviate the acute lung injury induced by lipopolysaccharide, and its effects might be related to lessening the adhesion molecules.
authors.affiliation: Institute of Microcirculation, Hebei North University, Zhangjiakou, China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: Institute of Microcirculation, Hebei North University, Zhangjiakou, China.
authors.forename: Zi-gang
authors.initials: ZG
authors.lastname: Zhao
authors.affiliation: Institute of Microcirculation, Hebei North University, Zhangjiakou, China.
authors.forename: Chun-yu
authors.initials: CY
authors.lastname: Niu
authors.affiliation: Institute of Microcirculation, Hebei North University, Zhangjiakou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008070:Lipopolysaccharides; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008196:Lymph / Q000378:metabolism*; D008297:Male; D019007:P-Selectin / Q000032:analysis; D009195:Peroxidase / Q000032:analysis; D011897:Random Allocation; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D008070:Lipopolysaccharides; D019007:P-Selectin; D018799:Intercellular Adhesion Molecule-1; D009195:Peroxidase