title: Proteomic analysis of Rhizoctonia solani AG-1 sclerotia maturation.
abstract: Rhizoctonia solani (R. solani), a soil-borne necrotrophic pathogen, causes various plant diseases. Rhizoctonia solani is a mitosporic fungus, the sclerotium of which is the primary inoculum and ensures survival of the fungus during the offseason of the host crop. Since the fungus does not produce any asexual or sexual spores, understanding the biology of sclerotia is important to examine pathogen ecology and develop more efficient methods for crop protection. Here, one- and two-dimensional gel electrophoresis (1-DE and 2-DE, respectively) were used to examine protein regulation during the maturation of fungal sclerotia. A total of 75 proteins (20 proteins from 1-DE using matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF) mass spectrometry (MS) and 55 proteins from 2-DE using MALDI-TOF MS or MALDI-TOF/TOF MS) were differentially expressed during sclerotial maturation. The identified proteins were classified into ten categories based on their biological functions, including genetic information processing, carbohydrate metabolism, cell defense, amino acid metabolism, nucleotide metabolism, cellular processes, pathogenicity and mycotoxin production, and hypothetical or unknown functions. Interestingly, two vacuole function-related proteins were highly up-regulated throughout sclerotial maturation, which was confirmed at the transcript level by reverse transcriptase polymerase chain reaction (RT-PCR) analysis. These findings contribute to our understanding of the biology of R. solani sclerotia.
authors.affiliation: Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Young Sang
authors.initials: YS
authors.lastname: Kwon
authors.affiliation: Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Sang Gon
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Chung
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsang 712-749, Republic of Korea.
authors.forename: Hanhong
authors.initials: H
authors.lastname: Bae
authors.affiliation: Department of Chemistry, Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Jeong
authors.affiliation: Department of Chemistry, Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin
authors.affiliation: Bio-crop Development Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Mi-Jeong
authors.initials: MJ
authors.lastname: Jeong
authors.affiliation: Bio-crop Development Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Soo-Chul
authors.initials: SC
authors.lastname: Park
authors.affiliation: Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Youn-Sig
authors.initials: YS
authors.lastname: Kwak
authors.affiliation: Central Instrument Facility, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: bdwon@gnu.ac.kr.
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Bae
authors.affiliation: Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: ylee@gnu.ac.kr.
authors.forename: Yong Bok
authors.initials: YB
authors.lastname: Lee
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D040901:Proteomics; D012232:Rhizoctonia / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins
keywords: 2-DE; Fungus; MALDI-TOF MS; Pathogen; Protein