title: Salivary auto-antibodies as noninvasive diagnostic markers of oral cavity squamous cell carcinoma.
abstract: BACKGROUND
  Oral cavity squamous cell carcinoma (OSCC) is one of the most common cancers worldwide, and its incidence is still increasing. Approximately 50% of patients with OSCC die within 5 years after diagnosis, mostly ascribed to the fact that the majority of patients present advanced stages of OSCC at the time of diagnosis.
  
  
  METHODS
  To discover salivary biomarkers for ameliorating the detection of OSCC, herein, we developed a multiplexed bead-based platform to simultaneously detect auto-antibodies (auto-Abs) in salivary samples.
  
  
  RESULTS
  Compared with healthy individuals, the salivary levels of anti-p53, anti-survivin, anti-Hsp60, and anti-RPLP0 were significantly elevated in patients with OSCC. Noteworthily, the elevated levels of anti-p53, anti-survivin, and anti-Hsp60 were already observed in individuals with oral potentially malignant disorder. Moreover, the salivary levels of anti-p53, anti-survivin, anti-Hsp60, anti-RPLP0, and anti-CK8 were significantly elevated in patients with early-stage OSCC compared with those in healthy individuals. Most importantly, the use of a combined panel of salivary anti-p53, anti-survivin, anti-Hsp60, and anti-RPLP0 largely improves the detection of OSCC.
  
  
  CONCLUSIONS
  Collectively, our results reveal that the salivary auto-Abs are effective OSCC biomarkers and the four-auto-Ab panel provides a novel and practicable approach for OSCC screening.
  
  
  CONCLUSIONS
  This study provides the first evidence for the potential clinical application of salivary auto-Abs in OSCC diagnosis.
authors.affiliation: Departments of Medical Biotechnology and Laboratory Science, College of Medicine, and Molecular Medicine Research Center, Chang Gung University, Tao-Yuan; luckywu@mail.cgu.edu.tw bigfanfan@yahoo.com.tw.
authors.forename: Chih-Ching
authors.initials: CC
authors.lastname: Wu
authors.affiliation: Molecular Medicine Research Center, Chang Gung University, Tao-Yuan;
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Chang
authors.affiliation: Molecular Medicine Research Center, Chang Gung University, Tao-Yuan; Department of Otolaryngology-Head and Neck Surgery, Chang Gung Memorial Hospital, Lin-Kou;
authors.forename: Kai-Ping
authors.initials: KP
authors.lastname: Chang
authors.affiliation: Molecular Medicine Research Center, Chang Gung University, Tao-Yuan;
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Department of Veterinary Medicine, National Chung Hsing University, Taichung;
authors.forename: Hao-Ping
authors.initials: HP
authors.lastname: Liu
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Liouying; and.
authors.forename: I-Ling
authors.initials: IL
authors.lastname: Lee
authors.affiliation: Cell and Molecular Biology, College of Medicine; Molecular Medicine Research Center, Chang Gung University, Tao-Yuan;
authors.forename: Jau-Song
authors.initials: JS
authors.lastname: Yu
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Liouying; and School of Dentistry, National Yang-Ming University, Taipei, Taiwan luckywu@mail.cgu.edu.tw bigfanfan@yahoo.com.tw.
authors.forename: Wei-Fan
authors.initials: WF
authors.lastname: Chiang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000912:Antibodies, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006801:Humans; D007118:Immunoassay; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000276:immunology; D012463:Saliva / Q000737:chemistry* / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000912:Antibodies, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor