title: Microencapsulation of lectin anti-cancer agent and controlled release by alginate beads, biosafety approach.
abstract: Hepatocellular carcinoma (HCC) is considered as one of the most aggressive cancer worldwide. In Egypt, the prevalence of HCC is increasing during last years. Recently, drug-loaded microparticles were used to improve the efficiency of various medical treatments. This study is designed to evaluate the anticancer potentialities of lectins against HCC while hinting to its safety usage. The aim is also extended to encapsulate lectins in alginate microbeads for oral drug delivery purposes. The extracted lectins showed anti-proliferative effect against HCC with a percentage of 60.76% by using its nontoxic dose with an up-regulation of P53 gene expression. Concerning the handling of lectin alginate microbeads for oral drug delivery, the prepared lectin alginate beads were ∼100μm in diameter. The efficiency of the microcapsules was checked by scanning electron microscopy, the SEM showed the change on the alginate beads surface revealing the successful lectin encapsulation. The release of lectins from the microbeads depended on a variety of factors as the microbeads forming carriers and the amount-encapsulated lectins. The Pisum sativum extracted lectins may be considered as a promising agent in controlling HCC and this solid dosage form could be suitable for oral administration complemented with/or without the standard HCC drugs.
authors.affiliation: Polymer Materials Research Department, Institute of Advanced Technology and New Material, City of Scientific Research and Technology Applications, New Borg El-Arab City 21934, Alexandria, Egypt. Electronic address: mohamed_elaassar@yahoo.com.
authors.forename: M R
authors.initials: MR
authors.lastname: El-Aassar
authors.affiliation: Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications, New Borg El-Arab City 21934, Alexandria, Egypt.
authors.forename: Elsayed E
authors.initials: EE
authors.lastname: Hafez
authors.affiliation: Biopharmaceutical Product Research Department, Genetic Engineering and Biotech. Institute, City of Scientific Research and Technology Applications, New Borg El-Arab City 21934, Alexandria, Egypt.
authors.forename: Nehal M
authors.initials: NM
authors.lastname: El-Deeb
authors.affiliation: Petrochemical Research Chair, Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; Textile Research Division, National Research Center, P.O. Box 12622, Dokki, Giza 12522, Egypt. Electronic address: m_gaballa@yahoo.com.
authors.forename: Moustafa M G
authors.initials: MM
authors.lastname: Fouda
mesh_terms: D000464:Alginates / Q000009:adverse effects* / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D002214:Capsules; D049109:Cell Proliferation / Q000187:drug effects; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000009:adverse effects* / Q000737:chemistry*; D020723:Glucuronic Acid / Q000009:adverse effects / Q000737:chemistry; D056945:Hep G2 Cells; D006603:Hexuronic Acids / Q000009:adverse effects / Q000737:chemistry; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D008863:Microspheres; D012449:Safety
chemical_list: D000464:Alginates; D000970:Antineoplastic Agents; D002214:Capsules; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006603:Hexuronic Acids; D037102:Lectins; D020723:Glucuronic Acid
keywords: Alginate beads; Encapsulation; Lectin