title: Association of an insertion/deletion polymorphism in IL1A 3'-UTR with risk for cervical carcinoma in Chinese Han Women.
abstract: Emerging evidence has demonstrated that polymorphisms of interleukin-1 (IL-1) may be involved in human tumorigenesis by regulating the production of this cytokine. Previous studies have investigated the association between two genetic variants (rs3783553 and rs17561) of IL1A and many diseases. The present study was conducted to evaluate whether these two variants are associated with cervical carcinoma (CC). These two polymorphisms were genotyped in 319 CC patients and 424 healthy controls by polymerase chain reaction polyacrylamide gel electrophoresis (PCR-PAGE) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Significantly reduced CC risk was observed to be associated with the insertion allele of rs3783553 (P=0.014, OR=0.71, 95% CI=0.57-0.88). Stratification analysis based on different certain clinical features showed that patients with the heterozygous genotype were associated with a reduced predisposition advancing to clinical stage II-III or developing non-squamous cell carcinoma. Furthermore, patients with the insertion homozygous genotype were also associated with a reduced risk to have a poor tumor differentiation. No significant association was observed between rs17561 and CC. The present study provided evidence that the rs3783553 in IL1A 3'-UTR is inversely associated with CC risk, suggesting an important role IL-1α may play in cervical carcinogenesis.
authors.affiliation: Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Laboratory of Molecular Translational Medicine, West China Institute of Women and Children's Health, Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, PR China; Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Laboratory of Molecular Translational Medicine, West China Institute of Women and Children's Health, Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Kui
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Laboratory of Molecular Translational Medicine, West China Institute of Women and Children's Health, Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Kana
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Quan
authors.affiliation: Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Mingrong
authors.initials: M
authors.lastname: Xi
authors.affiliation: Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, PR China; Laboratory of Molecular Translational Medicine, West China Institute of Women and Children's Health, Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, PR China. Electronic address: zhanglin@scu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000483:Alleles; D044466:Asian People; D002277:Carcinoma / Q000208:ethnology / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D054643:INDEL Mutation; D053582:Interleukin-1alpha / Q000235:genetics* / Q000276:immunology; D008875:Middle Aged; D009367:Neoplasm Staging; D011110:Polymorphism, Genetic; D002583:Uterine Cervical Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000276:immunology / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D053582:Interleukin-1alpha
keywords: Cervical carcinoma; Genetic susceptibility; IL1A; Polymorphism; rs3783553