title: A manual method to obtain platelet rich plasma.
abstract: OBJECTIVE
  This study is to report a manual method to obtain platelet rich plasma (PRP).
  
  
  METHODS
  For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.
  
  
  RESULTS
  A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.
  
  
  CONCLUSIONS
  The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor. Level of Evidence IV, Controlled Laboratory Study.
authors.affiliation: Hospital do Coração , Knee Institute, São Paulo, SP, Brazil, Knee Institute, Hospital do Coração (HCor), São Paulo, SP, Brazil.
authors.forename: Fabiana Paulino
authors.initials: FP
authors.lastname: Marques
authors.affiliation: Hospital do Coração , Knee Institute, São Paulo, SP, Brazil, Knee Institute, Hospital do Coração (HCor), São Paulo, SP, Brazil ; Universidade Federal de São Paulo, Escola Paulista de Medicina, Department of Orthopedics and Traumatology, São Paulo, SP, Brazil, Department of Orthopedics and Traumatology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Sheila Jean McNeill
authors.initials: SJ
authors.lastname: Ingham
authors.affiliation: Hospital do Coração , Knee Institute, São Paulo, SP, Brazil, Knee Institute, Hospital do Coração (HCor), São Paulo, SP, Brazil.
authors.forename: Andrea
authors.initials: A
authors.lastname: Forgas
authors.affiliation: Universidade Federal de São Paulo, Escola Paulista de Medicina, Department of Orthopedics and Traumatology, São Paulo, SP, Brazil, Department of Orthopedics and Traumatology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Carlos Eduardo da Silveira
authors.initials: CE
authors.lastname: Franciozi
authors.affiliation: Hospital do Coração , Knee Institute, São Paulo, SP, Brazil, Knee Institute, Hospital do Coração (HCor), São Paulo, SP, Brazil ; Universidade Federal de São Paulo, Escola Paulista de Medicina, Department of Orthopedics and Traumatology, São Paulo, SP, Brazil, Department of Orthopedics and Traumatology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Pedro Henrique
authors.initials: PH
authors.lastname: Sasaki
authors.affiliation: Hospital do Coração , Knee Institute, São Paulo, SP, Brazil, Knee Institute, Hospital do Coração (HCor), São Paulo, SP, Brazil ; Universidade Federal de São Paulo, Escola Paulista de Medicina, Department of Orthopedics and Traumatology, São Paulo, SP, Brazil, Department of Orthopedics and Traumatology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Rene Jorge
authors.initials: RJ
authors.lastname: Abdalla
keywords: Blood platelets; Methods.; Platelet-rich plasma