title: Analysis and pharmacokinetic study of curdione in Rhizoma Curcumae by UPLC/QTOF/MS.
abstract: An ultra-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC/QTOF/MS) method was established to determine the chemical components of curcuma rhizomes (Ezhu) and their pharmacokinetics. Chromatographic separation was performed by UPLC using a 1.8 µm column in order to obtain good resolution and increase the sensitivity of analysis. Accurate mass measurement within 5 ppm error for each ion produced in positive mode electrospray ionization and the subsequent QTOF product ions enabled 12 compounds to be identified. Several of the identified components, including β-elemene, curcumol, germacrone and curdione, are thought to be the biologically active ingredients. Quantitative pharmacokinetic analysis was also carried out by UPLC/QTOF/MS. Using 20(S)-protopanoxadiol as an internal standard, samples were prepared by protein precipitation with methanol. Chromatographic separation was performed on an Agilent Extend-C18 column (2.1 × 50 mm, 1.8 µm) with acetonitrile (0.1% formic acid)-water (0.1% formic acid) for gradient elution. Curdione calibration plots were linear over the range of 0.1-12.2 µg/mL for curdione in plasma with the lower quantification limit being 6.5 ng/mL, and the recovery from plasma was about 105.2%. The RSD for both intra- and inter-day precision was <9.9%.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, No. 325 Guohe Road, Shanghai, 200433, China; Department of Pharmaceutical Analysis Center, School of Pharmacy, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Diya
authors.initials: D
authors.lastname: Lv
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Chen
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Lou
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Chai
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D030024:Curcuma / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry*; D045788:Sesquiterpenes, Germacrane / Q000097:blood* / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010936:Plant Extracts; D045788:Sesquiterpenes, Germacrane; C504126:curdione
keywords: UPLC/QTOF/MS; curdione; pharmacokinetics; qualitative