title: Propofol prevents lung injury after intestinal ischemia-reperfusion by inhibiting the interaction between mast cell activation and oxidative stress.
abstract: OBJECTIVE
  Both mast cells and oxidative stress are involved in acute lung injury (ALI) induced by intestinal ischemia-reperfusion (IIR). The aim of this study was to investigate whether propofol could improve IIR-induced ALI through inhibiting their interaction.
  
  
  METHODS
  Repetitive, brief IIR or IIR+compound 48/80 was performed in adult Sprague-Dawley rats pretreated with saline, apocynin or propofol. And their lungs were excised for histology, ELISA and protein-expression measurements 2h after reperfusion.
  
  
  RESULTS
  Rats pretreated with saline developed critical ALI 2h after IIR. We found significant elevations in lung injury scores, lung wet/dry ratio and gp91phox, p47phox, intercellular cell adhesion molecule-1 protein expressions and higher level of malondialdehyde, interleukin-6 contents, and myeloperoxidase activities, as well as significant reductions in superoxide dismutase activities, accompanied with increases in mast cell degranulation evidenced by significant increases in mast cell counts, β-hexosaminidase concentrations, and tryptase expression. And the lung injury was aggravated in the presence of compound 48/80. However, pretreated with propofol and apocynin not only ameliorated the IIR-mediated pulmonary changes beyond the biochemical changes but also reversed the changes that were aggravated by compound 48/80.
  
  
  CONCLUSIONS
  Propofol protects against IIR-mediated ALI, most likely by inhibiting the interaction between oxidative stress and mast cell degranulation.
authors.affiliation: Department of Anesthesiology, The First People's Hospital of Foshan, 81 North of Rinlan Road, Foshan 528000, China; Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Weicheng
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Shaoli
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Yao
authors.affiliation: Department of Anesthesiology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 54 South of Xianlie Road, Guangzhou 510060, China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Gan
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Guangjie
authors.initials: G
authors.lastname: Su
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. Electronic address: heiziqing@sina.com.
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Hei
mesh_terms: D000098:Acetophenones / Q000494:pharmacology; D055371:Acute Lung Injury / Q000517:prevention & control*; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006993:Hypnotics and Sedatives / Q000494:pharmacology; D007422:Intestines / Q000098:blood supply; D008407:Mast Cells / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D000098:Acetophenones; D006993:Hypnotics and Sedatives; D003189:p-Methoxy-N-methylphenethylamine; C056165:acetovanillone; D015742:Propofol
keywords: Acute lung injury; Apocynin; Intestinal ischemia–reperfusion; Mast cell degranulation; Oxidative stress; Propofol