title: Comparison of meniscal fibrochondrocyte and synoviocyte bioscaffolds toward meniscal tissue engineering in the dog.
abstract: Tissue engineering is a promising field of study toward curing the meniscal deficient stifle; however the ideal cell type for this task is not known. We describe here the extraction of synoviocytes and meniscal fibrochondrocytes from arthroscopic debris from six dogs, which were cultured as tensioned bioscaffolds to synthesize meniscal-like fibrocartilage sheets. Despite the diseased status of the original tissues, synoviocytes and meniscal fibrochondrocytes had high viability at the time of removal from the joint. Glycosaminoglycan and collagen content of bioscaffolds did not differ. Meniscal fibrochondrocyte bioscaffolds contained more type II collagen, but collagen deposition was disorganized, with only 30-40% of cells viable. The collagen of synoviocyte bioscaffolds was organized into sheets and bands and 80-90% of cells were viable. Autologous, diseased meniscal fibrochondrocytes and synoviocytes are plausible cell sources for future meniscal tissue engineering research, however cell viability of meniscal fibrochondrocytes in the tensioned bioscaffolds was low.
authors.affiliation: College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, 700 SW 30th St., Corvallis, OR 97331, USA.
authors.forename: George A
authors.initials: GA
authors.lastname: Ballard
authors.affiliation: College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, 700 SW 30th St., Corvallis, OR 97331, USA. Electronic address: jennifer.warnock@oregonstate.edu.
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Warnock
authors.affiliation: Linus Pauling Institute, Oregon State University, 307 Linus Pauling Science Center, Corvallis, OR 97331, USA.
authors.forename: Gerd
authors.initials: G
authors.lastname: Bobe
authors.affiliation: College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, 700 SW 30th St., Corvallis, OR 97331, USA.
authors.forename: Katja F
authors.initials: KF
authors.lastname: Duesterdieck-Zellmer
authors.affiliation: College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, 700 SW 30th St., Corvallis, OR 97331, USA.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Baker
authors.affiliation: College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, 700 SW 30th St., Corvallis, OR 97331, USA.
authors.forename: Wendy I
authors.initials: WI
authors.lastname: Baltzer
authors.affiliation: College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, 700 SW 30th St., Corvallis, OR 97331, USA.
authors.forename: Jesse
authors.initials: J
authors.lastname: Ott
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004285:Dogs / Q000293:injuries* / Q000601:surgery; D005260:Female; D051445:Fibrocartilage / Q000166:cytology* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D008297:Male; D008592:Menisci, Tibial / Q000601:surgery; D013583:Synovial Membrane / Q000166:cytology* / Q000378:metabolism; D000070600:Tibial Meniscus Injuries; D023822:Tissue Engineering / Q000379:methods / Q000662:veterinary*; D054457:Tissue Scaffolds / Q000662:veterinary*
chemical_list: D006025:Glycosaminoglycans; D003094:Collagen
keywords: Cell culture; Meniscus; Osteoarthritis; Synovium; Tissue engineering