title: Direct electrochemistry and electrocatalysis of hemoglobin in graphene oxide and ionic liquid composite film.
abstract: In this paper a novel sensing platform based on graphene oxide (GO), ionic liquid (IL) 1-ethyl-3-methylimidazolium tetrafluoroborate and Nafion for the immobilization of hemoglobin (Hb) was adopted with a carbon ionic liquid electrode (CILE) as the substrate electrode, which was denoted as Nafion/Hb-GO-IL/CILE. Spectroscopic results suggested that Hb molecules were not denatured in the composite. A pair of well-defined redox peaks appeared on the cyclic voltammogram, which was attributed to the realization of direct electron transfer of Hb on the electrode. Electrochemical behaviors of Hb entrapped in the film were carefully investigated by cyclic voltammetry with the electrochemical parameters calculated. Based on the catalytic ability of the immobilized Hb, Nafion/Hb-GO-IL/CILE exhibited excellent electrocatalytic behavior towards the reduction of different substrates such as trichloroacetic acid in the concentration range from 0.01 to 40.0mM with the detection limit as 3.12 μM (3σ), H2O2 in the concentration range from 0.08 to 635.0 μM with the detection limit as 0.0137 μM (3σ) and NaNO2 in the concentration range from 0.5 to 800.0 μM with the detection limit as 0.0104 μM (3σ). So the proposed bioelectrode could be served as a new third-generation electrochemical sensor without mediator.
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China. Electronic address: swyy26@hotmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Shixing
authors.initials: S
authors.lastname: Gong
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Shi
authors.affiliation: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Cao
authors.affiliation: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Luyang
authors.initials: L
authors.lastname: Ling
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Weizhe
authors.initials: W
authors.lastname: Zheng
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Wencheng
authors.initials: W
authors.lastname: Wang
mesh_terms: D002384:Catalysis; D055664:Electrochemical Techniques; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D052578:Ionic Liquids / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry
chemical_list: D005465:Fluorocarbon Polymers; D006454:Hemoglobins; D055767:Immobilized Proteins; D052578:Ionic Liquids; D010087:Oxides; C040402:perfluorosulfonic acid; D006108:Graphite; D006861:Hydrogen Peroxide
keywords: Carbon ionic liquid electrode; Electrochemistry; Graphene oxide; Hemoglobin; Ionic liquid