title: Mitochondrial APE1/Ref-1 suppressed protein kinase C-induced mitochondrial dysfunction in mouse endothelial cells.
abstract: Protein kinase C (PKC) induces mitochondrial dysfunction, which is an important pathological factor in cardiovascular diseases. The role of apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE1/Ref-1) on PKC-induced mitochondrial dysfunction has not been variously investigated. In this study, phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C, induced mitochondrial hyperpolarization and reactive oxygen species generation and also increased mitochondrial translocation of APE1/Ref-1. APE1/Ref-1 overexpression suppressed PMA-induced mitochondrial dysfunction. In contrast, gene silencing of APE1/Ref-1 increased the sensitivity of mitochondrial dysfunction. Moreover, mitochondrial targeting sequence (MTS)-fused APE1/Ref-1 more effectively suppressed PMA-induced mitochondrial dysfunctions. These results suggest that mitochondrial APE1/Ref-1 is contributed to the protective role to protein kinase C-induced mitochondrial dysfunction in endothelial cells.
authors.affiliation: Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea; Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea.
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Joo
authors.affiliation: Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea; Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea.
authors.forename: Yu Ran
authors.initials: YR
authors.lastname: Lee
authors.affiliation: Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea; Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea.
authors.forename: Myoung Soo
authors.initials: MS
authors.lastname: Park
authors.affiliation: Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea; Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea.
authors.forename: Sunga
authors.initials: S
authors.lastname: Choi
authors.affiliation: BioNano Health Guard Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Kyoungsook
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Sports Science, Chungnam National University, Daejeon 305-765, Republic of Korea.
authors.forename: Sang Ki
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea.
authors.forename: Cuk-Seong
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea; Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea.
authors.forename: Jin Bong
authors.initials: JB
authors.lastname: Park
authors.affiliation: Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea; Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea.
authors.forename: Byeong Hwa
authors.initials: BH
authors.lastname: Jeon
mesh_terms: D000818:Animals; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000378:metabolism*; D042783:Endothelial Cells / Q000502:physiology*; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008928:Mitochondria / Q000502:physiology*; D011493:Protein Kinase C / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D011493:Protein Kinase C; C486216:Apex1 protein, mouse; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase
keywords: APE1/Ref-1; Endothelial cell; Mitochondria; Mitochondrial membrane potential; Phorbol 12-myristate 13-acetate