title: Wolbachia detection in insects through LAMP: loop mediated isothermal amplification.
abstract: BACKGROUND
  The bacterium Wolbachia is a promising agent for the biological control of vector-borne diseases as some strains have the ability to block the transmission of key human disease-causing pathogens. Fast, accurate and inexpensive methods of differentiating between infected and uninfected insects will be of critical importance as field-based trials of Wolbachia-based bio-control become increasingly common.
  
  
  RESULTS
  We have developed a specific and sensitive method of detecting Wolbachia based on the isothermal DNA amplification. This technique can be performed in an ordinary heat block without the need for gel-based visualisation, and is effective for a wide variety of insect hosts.
  
  
  CONCLUSIONS
  Here we present the development of a rapid, highly sensitive and inexpensive method to detect Wolbachia in a variety of insect hosts, including key mosquito disease vectors.
authors.forename: Daniela da Silva
authors.initials: Dda S
authors.lastname: Gonçalves
authors.forename: Anna Paula Alvim
authors.initials: AP
authors.lastname: Cassimiro
authors.forename: Caroline Dantas
authors.initials: CD
authors.lastname: de Oliveira
authors.forename: Nilton Barnabé
authors.initials: NB
authors.lastname: Rodrigues
authors.affiliation: Grupo: Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor, Centro de Pesquisas René Rachou - Fundação Oswaldo Cruz, Av, Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brazil. luciano@cpqrr.fiocruz.br.
authors.forename: Luciano Andrade
authors.initials: LA
authors.lastname: Moreira
mesh_terms: D000818:Animals; D061046:Biological Control Agents; D007313:Insecta / Q000382:microbiology*; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D020577:Wolbachia / Q000302:isolation & purification*
chemical_list: D061046:Biological Control Agents