title: Preparation of lysozyme molecularly imprinted polymers and purification of lysozyme from egg white.
abstract: Molecular imprinting as a promising and facile separation technique has received much attention because of its high selectivity for target molecules. In this study, lysozyme molecularly imprinted polymers (Lys-MIPs) were successfully prepared by the entrapment method with lysozyme as the template molecule, acrylamide as the functional monomer and N,N-methylenebisacrylamide as the cross-linker. The removal of the template lysozyme from the molecularly imprinted polymers was investigated in detail by two methods. The synthesized Lys-MIPs were characterized by scanning electron microscopy and Fourier transform-infrared, and the adsorption capacity, selectivity and reproducibility of the Lys-MIPs were also evaluated. The maximum adsorption capacity reached 94.8 mg/g, which is twice that of nonmolecularly imprinted polymers, and satisfactory selectivity and reproducibility were achieved. Using the Lys-MIP column, lysozyme could be separated completely from egg white, with purity close to 100% and mass recovery of 98.2%. This illustrated that the synthesized Lys-MIPs had high specific recognition and selectivity to the template lysozyme when they were applied to a mixture of protein standards and a real sample.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Northwest University, Xi'an, 710069, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Wang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Dong
authors.forename: Quan
authors.initials: Q
authors.lastname: Bai
mesh_terms: D000327:Adsorption; D000818:Animals; D002645:Chickens; D004529:Egg White / Q000737:chemistry*; D054802:Molecular Imprinting; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D011108:Polymers; D009113:Muramidase
keywords: lysozyme; molecular recognition; molecularly imprinted polymer (MIP); protein separation