title: Microwave-assisted deuterium exchange: the convenient preparation of isotopically labelled analogues for stable isotope dilution analysis of volatile wine phenols.
abstract: This study reports the convenient, low cost, one-step synthesis of labelled analogues of six volatile phenols, guaiacol, 4-methylguaiacol, 4-ethylguaiacol, 4-ethylphenol, eugenol and vanillin, using microwave-assisted deuterium exchange, for use as internal standards for stable isotope dilution analysis. The current method improves on previous strategies in that it enables incorporation of deuterium atoms on the aromatic ring, thereby ensuring retention of the isotope label during mass spectrometry fragmentation. When used as standards for SIDA, these labelled volatile phenols will improve the accuracy and reproducibility of quantitative food and beverage analysis.
authors.affiliation: The University of Adelaide, School of Agriculture, Food and Wine, PMB 1, Glen Osmond, SA 5064, Australia.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Crump
authors.affiliation: The University of Adelaide, School of Agriculture, Food and Wine, PMB 1, Glen Osmond, SA 5064, Australia.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Sefton
authors.affiliation: The University of Adelaide, School of Agriculture, Food and Wine, PMB 1, Glen Osmond, SA 5064, Australia. Electronic address: kerry.wilkinson@adelaide.edu.au.
authors.forename: Kerry L
authors.initials: KL
authors.lastname: Wilkinson
mesh_terms: D001547:Benzaldehydes; D003408:Cresols; D003903:Deuterium; D005054:Eugenol; D006139:Guaiacol / Q000031:analogs & derivatives; D007201:Indicator Dilution Techniques; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D008872:Microwaves; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D055549:Volatile Organic Compounds / Q000032:analysis*; D014920:Wine / Q000032:analysis*
chemical_list: D001547:Benzaldehydes; D003408:Cresols; D010636:Phenols; D055549:Volatile Organic Compounds; D005054:Eugenol; D006139:Guaiacol; C042291:4-ethylphenol; D003903:Deuterium; C084991:4-ethylguaiacol; C100058:vanillin; C025390:creosol
keywords: Isotope labelling; Microwave-assisted deuterium exchange; Volatile phenols