title: Molecular identification of Sarcocystis hominis in native cattle of central Iran: a case report.
abstract: Sarcocystis spp. are two-host protozoan parasites belonging to the phylum Apicomplexa. Among different known species of Sarcocystis in cattle, only Sarcocystis hominis is important from the public health viewpoint, because of its zoonotic characteristics. This study presents the first molecular identification of S. hominis in native cattle in central Iran. A sample of diaphragm muscle from a 6-year-old native cow slaughtered at Yazd Slaughterhouse, Yazd, central Iran, was collected in May 2013. DNA extraction was performed, using the salting-out method. DNA purification and precipitation were performed consecutively. The amplicon and digestion results were analyzed using agarose gel electrophoresis. A PCR product with 926 bp in length was obtained after amplification, and 376 bp and 550 bp in length after digestion that identified S. hominis. To the best of our knowledge, this study is the first of its kind to be reported from Iran.
authors.affiliation: Department of Food Hygiene and Safety, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: B
authors.initials: B
authors.lastname: Hajimohammadi
authors.affiliation: Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: G
authors.initials: G
authors.lastname: Eslami
authors.affiliation: Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Oryan
authors.affiliation: Department of Food Hygiene and Safety, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Zohourtabar
authors.affiliation: Yazd Veterinary Office, Yazd, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Pourmirzaei Tafti
authors.affiliation: Department of Food Hygiene and Safety, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Moghaddam Ahmadi
mesh_terms: D000003:Abattoirs; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D016054:DNA, Protozoan / Q000235:genetics; D003964:Diaphragm / Q000469:parasitology; D005260:Female; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012522:Sarcocystis / Q000235:genetics / Q000302:isolation & purification*; D012523:Sarcocystosis / Q000175:diagnosis* / Q000469:parasitology; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S