title: Facile synthesis of PSMA-g-3ABA/MWCNTs nanocomposite as a substrate for hemoglobin immobilization: application to catalysis of H(2)O(2).
abstract: The new nanocomposite films based on poly(styrene-alternative-maleic anhydride) grafted to 3-aminobenzoic acid (PSMA-g-3ABA) and multi-walled carbon nanotubes (MWCNTs) were applied to immobilize hemoglobin (Hb) for biosensor fabrication (PSMA-g-3ABA/MWCNTs). Electrochemical impedance spectroscopy was used to confirm the adsorption of Hb onto the surface of PSMA-g-3ABA/MWCNTs. The immobilized Hb maintains its bioactivities and displays an excellent electrochemical behavior. The biosensor was used to catalyze the reduction of hydrogen peroxide. The electrocatalytic response showed a linear dependence on the H2O2 concentration ranging widely from 1.0×10(-6)M to 5.0×10(-4)M with a detection limit of 3.2×10(-7)M. The apparent Michaelis-Menten constant of Hb on the modified electrode was estimated to be 0.22mM. The proposed method opens a way to develop biosensors by using nanostructured materials with low electrical conductivity.
authors.affiliation: Department of Chemistry, Faculty of Science, Hakim Sabzevari University, P.O. Box 397, Sabzevar, Iran. Electronic address: m.baghayeri@hsu.ac.ir.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Baghayeri
authors.affiliation: Department of Polymer Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Nazarzadeh Zare
authors.affiliation: Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Hasanzadeh
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002384:Catalysis; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006454:Hemoglobins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry*; D057230:Limit of Detection; D008298:Maleates / Q000138:chemical synthesis*; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011137:Polystyrenes / Q000138:chemical synthesis*; D062368:meta-Aminobenzoates / Q000138:chemical synthesis*
chemical_list: D001672:Biocompatible Materials; D006454:Hemoglobins; D055767:Immobilized Proteins; D008298:Maleates; D037742:Nanotubes, Carbon; D011137:Polystyrenes; D062368:meta-Aminobenzoates; C520889:poly(styrene-alt-maleic anhydride); D006861:Hydrogen Peroxide; C044855:3-aminobenzoic acid
keywords: Biosensor; Electrocatalysis; Hemoglobin; Nanocomposite; Poly(styrene-alternative-maleic anhydride)