title: Genetic architecture of limit dextrinase inhibitor (LDI) activity in Tibetan wild barley.
abstract: BACKGROUND
  Limit dextrinase inhibitor (LDI) inhibits starch degradation in barley grains during malting because it binds with limit dextrinase (LD). There is a wide genetic variation in LDI synthesis and inactivation during barley grain development and germination. However, the genetic control of LDI activity remains little understood.
  
  
  RESULTS
  In this study, association analysis was performed on 162 Tibetan wild accessions by using LDI activity, 835 Diversity Arrays Technology (DArT) markers and single nucleotide polymorphisms (SNPs) of the gene HvLDI encoding LDI. Two DArT markers, bpb-8347, bpb-0068, and 31 SNPs of HvLDI were significantly associated with LDI activity, explaining 10.0%, 6.6% and 13.4% of phenotypic variation, respectively. Bpb-8347 is located on chromosome 6H, near the locus of HvLDI, and bpb-0068 is located on 3H.
  
  
  CONCLUSIONS
  The current results confirmed the locus of the gene controlling LDI activity and identified a new DArT markers associated with LDI activity. The SNPs associated with LDI activity may provide a new insight into the genetic variation of LDI activity in barley grains.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Huang
authors.forename: Shengguan
authors.initials: S
authors.lastname: Cai
authors.forename: Lingzhen
authors.initials: L
authors.lastname: Ye
authors.forename: Yong
authors.initials: Y
authors.lastname: Han
authors.forename: Dezhi
authors.initials: D
authors.lastname: Wu
authors.forename: Fei
authors.initials: F
authors.lastname: Dai
authors.forename: Chengdao
authors.initials: C
authors.lastname: Li
authors.affiliation: Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China. zhanggp@zju.edu.cn.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
mesh_terms: D060146:Ecotype; D005787:Gene Frequency / Q000235:genetics; D005828:Genetics, Population; D055106:Genome-Wide Association Study; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006239:Haplotypes / Q000235:genetics; D001467:Hordeum / Q000201:enzymology* / Q000235:genetics*; D040582:Inheritance Patterns / Q000235:genetics; D015810:Linkage Disequilibrium / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018609:Tibet
chemical_list: D010940:Plant Proteins; D006026:Glycoside Hydrolases; C100181:pullulanase