title: Silencing of HIF-1α enhances the radiation sensitivity of human glioma growth in vitro and in vivo.
abstract: Gliomas are the leading cause of cancer-related mortality worldwide, and the incidence is increasing. Because gliomas often become resistant to radiation treatment, it is urgent to develop novel therapeutic methods that are more effective and less toxic than current therapies so as to enhance patient survival and quality of life. Effective enhancement of radiation therapy for gliomas in vivo and in vitro was observed upon silencing of hypoxia-inducible factor 1α (HIF-1α) with RNA interference; this enhancement was related to changes in the cell cycle and apoptosis that were accompanied by modulation of Cdc2, cyclin B1, and Bcl-2 expression. Our data suggest that HIF-1α silencing combined with radiation therapy will increase the therapeutic efficacy of glioma treatment via regulation of cell cycle and apoptosis-related signaling pathways.
authors.affiliation: Department of Radiology, Xijing Hospital, Fourth Military Medical University, China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Oncology, The 2nd Affiliated Hospital, Xian Jiaotong University, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Bai
authors.affiliation: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Xijing Hospital, Fourth Military Medical University, China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, The 3rd Hospital of People Liberation Army, China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, China.
authors.forename: Sanzhong
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Radiology, Xijing Hospital, Fourth Military Medical University, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huan
authors.affiliation: Department of Radiology, Xijing Hospital, Fourth Military Medical University, China.
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, China. Electronic address: weidcao@126.com.
authors.forename: Weidong
authors.initials: W
authors.lastname: Cao
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000532:radiotherapy; D045744:Cell Line, Tumor; D005260:Female; D020868:Gene Silencing / Q000502:physiology*; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000532:radiotherapy; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000172:deficiency* / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D011836:Radiation Tolerance / Q000502:physiology*; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit
keywords: Gliomas; HIF-1α; Pathway; RNAi; Radiation sensitivity