title: A mitochondria-targeted turn-on fluorescent probe based on a rhodol platform for the detection of copper(I).
abstract: A new spirocyclized rhodol-based fluorescent probe has been developed for detecting mitochondrial Cu(+). Alkylation of the hydroxy group of a xanthene moiety with a tris(2-pyridylmethyl)amine-based ligand induced the formation of a non-fluorescent spirocyclic structure. The reaction with Cu(+) in the presence of submillimolar concentrations of glutathione at physiological pH resulted in the elimination of the ligand together with an increase in the fluorescence of the rhodol fluorophore. This probe was used to visualize mitochondrial Cu(+) in copper supplemented cells.
authors.affiliation: Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8602, Japan. taki@itbm.nagoya-u.ac.jp.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Taki
authors.forename: Kazushi
authors.initials: K
authors.lastname: Akaoka
authors.forename: Koji
authors.initials: K
authors.lastname: Mitsui
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D003300:Copper