title: The analysis of correlation between IL-1B gene expression and genotyping in multiple sclerosis patients.
abstract: IL-1B is released by monocytes, astrocytes and brain endothelial cells and seems to be involved in inflammatory reactions of the central nervous system (CNS) in multiple sclerosis (MS). This study aims to evaluate the expression level of IL-1B mRNA in peripheral blood mononuclear cells (PBMCs), genotype the rs16944 SNP and find out the role of this SNP on the expression level of IL-1B in MS patients. We found that the expression level of IL-1B in MS patients increased 3.336 times more than controls in PBMCs but the rs16944 SNP in the promoter region of IL-1B did not affect the expression level of this gene and there was not association of this SNP with MS in the examined population. Also, our data did not reveal any correlation between normalized expressions of IL-1B gene with age of participants, age of onset, and disease duration.
authors.affiliation: Department of Basic Sciences, Gonabad University of Medical Sciences, Gonabad, Islamic Republic of Iran.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Heidary
authors.affiliation: Department of Molecular Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Islamic Republic of Iran.
authors.forename: Nahid
authors.initials: N
authors.lastname: Rakhshi
authors.affiliation: Department of Basic Sciences, Gonabad University of Medical Sciences, Gonabad, Islamic Republic of Iran; Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Pahlevan Kakhki
authors.affiliation: Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Behmanesh
authors.affiliation: Department of Medical Genetics, National Research Institute of Genetic Engineering and Biotechnology, Tehran, Islamic Republic of Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Sanati
authors.affiliation: Department of Biology, Faculty of Basic Sciences, Zabol University, Zabol, Islamic Republic of Iran; Faculty of Pharmacy and Pharmaceutical Science Research Center, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
authors.forename: Nima
authors.initials: N
authors.lastname: Sanadgol
authors.affiliation: Department of Biology, Faculty of Basic Sciences, Zabol University, Zabol, Islamic Republic of Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Kamaladini
authors.affiliation: Department of Molecular Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Islamic Republic of Iran. Electronic address: Abbasnikravesh@nkums.ac.ir.
authors.forename: Abbas
authors.initials: A
authors.lastname: Nikravesh
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D009103:Multiple Sclerosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D053583:Interleukin-1beta; D012333:RNA, Messenger
keywords: Cytokines; Gene expression; Genotyping; IL-1B; Inflammation; Multiple sclerosis