title: Sesquiterpene acids from Shellac and their bioactivities evaluation.
abstract: Seven sesquiterpenes, including four α-cedrene types (Shellolic acid A-C, Laccishellolic acid), two R-curcumene types (Shellolic acid D-E) and Shellolic acid F were isolated from Shellac, of which six were unknown. Their structures were elucidated by HR-ESI-MS, UV, IR, 1D and 2D NMR methods. The absolute configurations were confirmed via the Mosher's method, circular dichroism (CD) and optical rotation analyses. A plausible biogenetic relationship for 1-6 is proposed. Cytotoxic and anti-bacterial activities of the isolates were evaluated. To the best of our knowledge, none compounds showed any cytotoxic activity, which is well consistent with that Shellac used as nontoxic material in pharmaceutical formulations and food additives. However, compound 1 exhibited inhibitory activity against Bacillus subtilis with the MIC value of 0.1 mg/mL.
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Lu
authors.affiliation: School of Pharmacy, Shihezi University Shihezi 832002, China.
authors.forename: Hangyu
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: huang_jian1977@yahoo.com.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Shihezi University Shihezi 832002, China.
authors.forename: Guoyu
authors.initials: G
authors.lastname: Li
authors.affiliation: Mongolian Medicine College of Pharmacy, Inner Mongolia University for the Nationalities, Tongliao 028043, China.
authors.forename: Qinghu
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of life science and Biotechnology, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: School of life science and Biotechnology, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Shihezi University Shihezi 832002, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Pharmacy, Shihezi University Shihezi 832002, China. Electronic address: wangjh_716@163.com.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008826:Microbial Sensitivity Tests; D012116:Resins, Plant / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D012116:Resins, Plant; D012717:Sesquiterpenes; C000596992:shellolic acid A; C003934:shellac
keywords: Anti-bacterial; Anti-tumor; Sesquiterpene; Shellac; Shellolic acid A–F