title: Ny-1andNy-2genes conferring hypersensitive response to potato virus Y (PVY) in cultivated potatoes: mapping and marker-assisted selection validation for PVY resistance in potato breeding.
abstract: Potato virus Y (PVY) is one of the most important viruses affecting potato (Solanum tuberosum) production. In this study, a novel hypersensitive response (HR) gene,Ny-2, conferring resistance to PVY was mapped on potato chromosome XI in cultivar Romula. In cultivars Albatros and Sekwana, theNy-1gene was mapped on chromosome IX. In cv. Romula, the local lesions appeared in leaves inoculated with the PVYN-Wiisolate at 20 and 28 °C; PVY systemic infections were only occasionally observed at the higher temperature. In cvs. Albatros and Sekwana, expression of the necrotic reaction to virus infection was temperature-dependent. PVYN-Wiwas localized at 20 °C; at 28 °C, the systemic, symptomless infection was observed. We developed the B11.61600marker co-segregating withNy-2and the S1d11 marker specific for theNy-1gene. Fifty potato cultivars were tested with markers B11.6 and S1d11 and marker SC895 linked to theNy-1gene in cv. Rywal. These results indicated the utility of these markers for marker-assisted selection of HR-like PVY resistance in potato breeding programs.
authors.affiliation: Plant Breeding and Acclimatization Institute, National Research Institute, Platanowa 19, 05-831 Młochów, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Szajko
authors.affiliation: Plant Breeding and Acclimatization Institute, National Research Institute, Platanowa 19, 05-831 Młochów, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Strzelczyk-Żyta
authors.affiliation: Plant Breeding and Acclimatization Institute, National Research Institute, Platanowa 19, 05-831 Młochów, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Marczewski
keywords: Hypersensitivity; Mapping; Marker-assisted selection; PVY; Potato cultivars