title: A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1.
abstract: BACKGROUND
  Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp.
  
  
  RESULTS
  We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1.
  
  
  CONCLUSIONS
  pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.
authors.forename: Bahgat
authors.initials: B
authors.lastname: Fayed
authors.forename: Ellen
authors.initials: E
authors.lastname: Younger
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Taylor
authors.affiliation: Department of Biology, University of York, York YO10 5DD, UK. maggie.smith@york.ac.uk.
authors.forename: Margaret C M
authors.initials: MC
authors.lastname: Smith
mesh_terms: D001287:Attachment Sites, Microbiological / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D005822:Genetic Vectors / Q000378:metabolism*; D019426:Integrases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D019426:Integrases