title: Cucurbitacin-I, a natural cell-permeable triterpenoid isolated from Cucurbitaceae, exerts potent anticancer effect in colon cancer.
abstract: Cucurbitacin-I is a triterpenoids found in medicinal plants and have diverse pharmacological and biological activities. In this study, the antitumor effects of cucurbitacin-I on colon cancer and possible roles in apoptosis and cell cycle arrest were investigated. Treatment of SW480 cells, a human colon cancer cells, with cucurbitacin-I decreased cell viability and cell proliferation in a concentration-dependent manner. Also, cucurbitacin-I induced G2/M phase cell cycle arrest in SW480 cells with a decreased expression of cell cycle proteins including cyclin B1, cyclin A, CDK1, and CDC25C. Moreover, cucurbitacin-I induced increased cleavage of caspase-3, -7, -8, -9, and poly ADP ribose polymerase. When we examined the inhibitory effect of cucurbitacin-I on tumor growth in vivo, cucurbitacin-I effectively inhibited the tumorigenicity and growth of CT-26 cells in syngenic BALB/c mice. In summary, the present study showed that cucurbitacin-I reduced colon cancer cell proliferation by enhancing apoptosis and causing cell cycle arrest at the G2/M phase.
authors.affiliation: Department of Biomedical Science, Catholic University of Daegu, Gyeongsan-Si, Republic of Korea.
authors.forename: Hyeon Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Food and Nutrition, Hallym University, Chuncheon, Republic of Korea.
authors.forename: Jung Han Yoon
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Biomedical Science, Catholic University of Daegu, Gyeongsan-Si, Republic of Korea. Electronic address: toto0818@cu.ac.kr.
authors.forename: Jin-Kyung
authors.initials: JK
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D016203:CDC2 Protein Kinase; D020169:Caspases / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000502:physiology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000188:drug therapy*; D019662:Cucurbitaceae / Q000737:chemistry*; D019925:Cyclin A / Q000032:analysis / Q000378:metabolism; D056744:Cyclin B1 / Q000032:analysis / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000032:analysis / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000188:drug therapy; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D020687:cdc25 Phosphatases / Q000032:analysis / Q000378:metabolism
chemical_list: C534699:CCNB1 protein, human; D019925:Cyclin A; D056744:Cyclin B1; D014315:Triterpenes; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases; C517009:CDC25C protein, human; D020687:cdc25 Phosphatases; D020169:Caspases; C038106:cucurbitacin I
keywords: Apoptosis; CT-26 cells; Cucurbitacin-I; G2/M arrest; SW480 cells