title: Study of Coxsackie B viruses interactions with Coxsackie Adenovirus receptor and Decay-Accelerating Factor using Human CaCo-2 cell line.
abstract: BACKGROUND
  Decay Accelerating Factor (DAF) and Coxsackievirus-Adenovirus Receptor (CAR) have been identified as cellular receptors for Coxsackie B viruses (CV-B). The aim of this study is to elucidate the different binding properties of CV-B serotypes and to find out if there are any amino acid changes that could be associated to the different phenotypes.Twenty clinical CV-B isolates were tested on CaCo-2 cell line using anti-DAF (BRIC216) and anti-CAR (RmcB) antibodies. CV-B3 Nancy prototype strain and a recombinant strain (Rec, CV-B3/B4) were tested in parallel. The P1 genomic region of 12 CV-B isolates from different serotypes was sequenced and the Trans-Epithelial Electrical Resistance (TEER) along with the virus growth cycle was measured.
  
  
  RESULTS
  Infectivity assays revealed clear differences between CV-B isolates with regard to their interactions with DAF and CAR. All tested CV-B isolates showed an absolute requirement for CAR but varied in their binding to DAF. We also reported that for some isolates of CV-B, DAF attachment was not adapted. Genetic analysis of the P1 region detected multiple differences in the deduced amino acid sequences.
  
  
  CONCLUSIONS
  Within a given serotype, variations exist in the capacity of virus isolates to bind to specific receptors, and variants with different additional ligands may arise during infection in humans as well as in tissue culture.
authors.affiliation: Laboratory of Transmissible Diseases LR99-ES27, Faculty of Pharmacy, Avenue Avicenne 5000, Monastir, Tunisia. riabi_samira@yahoo.fr.
authors.forename: Samira
authors.initials: S
authors.lastname: Riabi
authors.forename: Rafik
authors.initials: R
authors.lastname: Harrath
authors.forename: Imed
authors.initials: I
authors.lastname: Gaaloul
authors.forename: Lamjed
authors.initials: L
authors.lastname: Bouslama
authors.forename: Dorsaf
authors.initials: D
authors.lastname: Nasri
authors.forename: Mahjoub
authors.initials: M
authors.lastname: Aouni
authors.forename: Sylvie
authors.initials: S
authors.lastname: Pillet
authors.forename: Bruno
authors.initials: B
authors.lastname: Pozzetto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018958:CD55 Antigens / Q000235:genetics* / Q000378:metabolism; D016466:CHO Cells; D018938:Caco-2 Cells; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein / Q000235:genetics* / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D029822:Enterovirus B, Human / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000235:genetics* / Q000821:virology; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D012703:Serotyping
chemical_list: D018958:CD55 Antigens; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D008024:Ligands