title: Macrophage colony-stimulating factor induces prolactin expression in rat pituitary gland.
abstract: We investigated the role of macrophage colony-stimulating factor (M-CSF) in the pituitary gland to understand the effect of M-CSF on pituitary hormones and the relationship between the endocrine and immune systems. When we attempted to establish pituitary cell lines from a thyrotropic pituitary tumor (TtT), a macrophage cell line, TtT/M-87, was established. We evaluated M-CSF-like activity in conditioned media (CM) from seven pituitary cell lines using TtT/M-87 cells. TtT/M-87 proliferation significantly increased in the presence of CM from TtT/GF cells, a pituitary folliculostellate (FS) cell line. M-CSF mRNA was detected in TtT/GF and MtT/E cells by reverse transcriptase-polymerase chain reaction (RT-PCR), and its expression in TtT/GF cells was increased in a lipopolysaccharide (LPS) dose-dependent manner. M-CSF mRNA expression was also increased in rat anterior pituitary glands by LPS. M-CSF receptor (M-CSFR) mRNA was only detected in TtT/ M-87 cells and increased in the LPS-stimulated rat pituitary glands. In rat pituitary glands, M-CSF and M-CSFR were found to be localized in FS cells and prolactin (PRL)-secreting cells, respectively, by immunohistochemistry. The PRL concentration in rat sera was significantly increased at 24 h after M-CSF administration, and mRNA levels significantly increased in primary culture cells of rat anterior pituitary glands. In addition, TNF-α mRNA was increased in the primary culture cells by M-CSF. These results revealed that M-CSF was secreted from FS cells and M-CSF regulated PRL expression in rat pituitary glands.
authors.affiliation: 1 Department of Regulation Biology, Faculty of Science, Saitama University,Sakuraku Saitama 338-8570, Japan.
authors.forename: Satoya
authors.initials: S
authors.lastname: Hoshino
authors.forename: Reiko
authors.initials: R
authors.lastname: Kurotani
authors.forename: Yuki
authors.initials: Y
authors.lastname: Miyano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sakahara
authors.forename: Kanako
authors.initials: K
authors.lastname: Koike
authors.forename: Minoru
authors.initials: M
authors.lastname: Maruyama
authors.forename: Fumio
authors.initials: F
authors.lastname: Ishikawa
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sakatai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Abe
authors.forename: Takafumi
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000818:Animals; D002460:Cell Line; D017077:Culture Media, Conditioned; D005786:Gene Expression Regulation / Q000502:physiology*; D008070:Lipopolysaccharides / Q000633:toxicity; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D010902:Pituitary Gland / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D011388:Prolactin / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; D016173:Macrophage Colony-Stimulating Factor; D011388:Prolactin; D016186:Receptor, Macrophage Colony-Stimulating Factor
keywords: M-CSF; M-CSF receptor; folliculo-stellate cells; pituitary gland; prolactin