title: Variation in the structural changes of myoglobin in the presence of several protic ionic liquid.
abstract: Protein stability in ionic solution depends on the delicate balance between protein-ion and ion-ion interactions. To address the ion specific effects on the protein, we have examined the stability of myoglobin (Mb) in the presence of buffer and ammonium-based ionic liquids (ILs) (50%, v/v). Here, fluorescence and circular dichroism (CD) spectroscopy experiments are used to study the influence of ILs on structure and stability of Mb. Our experimental results reveal that more viscous ILs (sulphate or phosphate ions) are stabilizers and therefore more biocompatible for Mb structure. Surprisingly, the less viscous ILs such as acetate anion based ILs are destabilizers for the native structure of Mb. Our results explicitly elucidate that anion variation has significant influence on Mb stability efficiency than cation variation. This study provides insight into anion effects on protein stability and explains that the intrasolvent interactions can be leveraged to enhance the stability.
authors.affiliation: Plasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Republic of Korea.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Attri
authors.affiliation: Department of Chemistry, University of Delhi, Delhi 110 007, India.
authors.forename: Indrani
authors.initials: I
authors.lastname: Jha
authors.affiliation: Plasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Republic of Korea.
authors.forename: Eun Ha
authors.initials: EH
authors.lastname: Choi
authors.affiliation: Department of Chemistry, University of Delhi, Delhi 110 007, India. Electronic address: venkatesup@hotmail.com.
authors.forename: Pannuru
authors.initials: P
authors.lastname: Venkatesu
mesh_terms: D064751:Ammonium Compounds / Q000737:chemistry; D000818:Animals; D052578:Ionic Liquids / Q000737:chemistry / Q000494:pharmacology*; D008958:Models, Molecular; D009211:Myoglobin / Q000737:chemistry*; D055550:Protein Stability / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D013696:Temperature
chemical_list: D064751:Ammonium Compounds; D052578:Ionic Liquids; D009211:Myoglobin
keywords: Circular dichroism; Fluorescence; Ionic liquid; Myoglobin; Protein stability