title: Chemical constituents from the stems of Gymnema sylvestre.
abstract: OBJECTIVE
  To study the chemical constituents of stems of Gymnema sylvestre (Retz.) Schult.
  
  
  METHODS
  Chromatographic techniques using silica gel, C18 reversed phase silica gel, and prep-HPLC were used. The structures were elucidated on the basis of MS and spectroscopic analysis (1D and 2D NMR), as well as chemical methods.
  
  
  RESULTS
  Seven compounds were isolated and their structures were elucidated as conduritol A (1), stigmasterol (2), lupeol (3), stigmasterol-3-O-β-D-glucoside (4), the sodium salt of 22α-hydroxy-longispinogenin-3-O-β-D-glucopyranosyl-(1→3)-β-D-glu-curono-pyranosyl-28-O-α-L-rhamnopyranoside (5), oleanolic acid-3-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (6), and the sodium salt of 22α-hydroxy-longispinogenin 3-O-β-D-glucuronopyranosyl-28-O-α-L-rhamnopyranoside (7). The inhibition activities of compounds 1, 5-7 on non-enzymatic glycation of protein in vitro were evaluated.
  
  
  CONCLUSIONS
  Compound 7 is a new triterpenoid saponin. It was shown that compounds 1, 5-7 have weak inhibition activities for non-enzymatic glycation of protein in vitro.
authors.affiliation: Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Traditional Chinese Medicine Chemistry, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Tun-Hai
authors.initials: TH
authors.lastname: Xu
authors.affiliation: School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
authors.forename: Man-Qi
authors.initials: MQ
authors.lastname: Zhang
authors.affiliation: Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China; School of Pharmacy, Jilin University, Changchun 130021, China. Electronic address: xyj6492@126.com.
authors.forename: Ya-Juan
authors.initials: YJ
authors.lastname: Xu
authors.affiliation: Department of General Internal Medicine, The First Hospital of Jilin University, Jilin University, Changchun 130021, China. Electronic address: yh_zy5@163.com.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Jiang
authors.affiliation: Department of Traditional Chinese Medicine Chemistry, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Tong-Hua
authors.initials: TH
authors.lastname: Liu
authors.affiliation: Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China.
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Xu
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D031104:Gymnema sylvestre / Q000737:chemistry*; D015394:Molecular Structure; D018547:Plant Stems / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Gymnema sylvestre; Non-enzymatic glycation-inhibiting activity; Triterpenoid saponin