title: The effect of nutrition pattern alteration on Chlorella pyrenoidosa growth, lipid biosynthesis-related gene transcription.
abstract: Heterotrophy to photoautotrophy transition leads to the accumulation of lipids in Chlorella, which has potential to produce both healthy food and biofuels. Therefore, it is of key interest to study the metabolism shift and gene expression changes that influenced by the transition. Both total and neutral lipids contents were increased rapidly within 48 h after the switch to light environment, from 24.5% and 18.0% to 35.3% and 27.4%, respectively, along with the sharp decline of starch from 42.3% to 10.4% during 24h photoinduction phase. By analyzing the correlation between lipid content and gene expression, results revealed several genes viz. me g3137, me g6562, pepc g6833, dgat g3280 and dgat g7566, which encode corresponding enzymes in the de novo lipid biosynthesis pathway, are highly related to lipid accumulation and might be exploited as target genes for genetic modification. These results represented the feasibility of lipid production through trophic converting cultivation.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: jhfan@ecust.edu.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: cuiyb2016@gmail.com.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: zhouyang597@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: wanminxi2012@gmail.com.
authors.forename: Minxi
authors.initials: M
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: wlwang@ecust.edu.cn.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: jlxie@ecust.edu.cn.
authors.forename: Jingli
authors.initials: J
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: ygli@ecust.edu.cn.
authors.forename: Yuanguang
authors.initials: Y
authors.lastname: Li
mesh_terms: D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics*; D002708:Chlorella / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D052836:Heterotrophic Processes / Q000187:drug effects / Q000235:genetics; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis*; D009584:Nitrogen / Q000494:pharmacology*; D010758:Phosphorus / Q000494:pharmacology*; D052817:Phototrophic Processes / Q000187:drug effects / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D008055:Lipids; D010758:Phosphorus; D051048:Diacylglycerol O-Acyltransferase; D009584:Nitrogen
keywords: Chlorella; Gene expression; Heterotrophy; Lipid; Photoautotrophy