title: The efficacy of Acanthamoeba cyst kill and effects upon contact lenses of a novel ultraviolet lens disinfection system.
abstract: OBJECTIVE
  To assess the efficacy of a novel ultraviolet (UV) lens device on the killing of Acanthamoeba cysts and the impact of efficacious doses of UV upon soft contact lens parameter and material characteristics.
  
  
  METHODS
  Prospective, in vitro, experimental study of a device.
  
  
  METHODS
  A UV lens device was constructed and used to expose Acanthamoeba cysts to various levels of UV irradiation. Once an efficacious dose, as defined by a greater than 3 log reduction, was determined (130 mJ/cm(2)), 6 soft contact lens materials (etafilcon A, senofilcon A, galyfilcon A, lotrafilcon A, polymacon, and comfilcon A) were exposed to that dose for 30 cycles and tested for visual parameters, mechanical parameters, and cytotoxicity.
  
  
  RESULTS
  The UV device produced an average log reduction of over 3.5 log of Acanthamoeba cysts when the lens and solution inside of the inset case was irradiated with 130 mJ per cm(2) of UV or greater. After 30 cycles of 130 mJ per cm(2) UV dose each, no gross changes were observed in mechanical properties or cytotoxicity tests in any soft contact lenses tested. In visual parameters, polymacon and lotrafilcon A exhibited a shift in sphere power and diameter, respectively.
  
  
  CONCLUSIONS
  The novel UV lens device was able to provide a marked log reduction to Acanthamoeba cysts, one of the most resistant ocular disease-causing organisms found in lens cases, without a detrimental effect on many lens materials.
authors.affiliation: OBRUS Microbiology Services, Department of Infection, Immunity & Inflammation, University of Leicester, Leicester, United Kingdom.
authors.forename: James
authors.initials: J
authors.lastname: Lonnen
authors.affiliation: Johnson & Johnson Vision Care, Inc, Jacksonville, Florida.
authors.forename: Karson S
authors.initials: KS
authors.lastname: Putt
authors.affiliation: Johnson & Johnson Vision Care, Inc, Jacksonville, Florida.
authors.forename: Edward R
authors.initials: ER
authors.lastname: Kernick
authors.affiliation: Johnson & Johnson Vision Care, Inc, Jacksonville, Florida. Electronic address: clakkis@its.jnj.com.
authors.forename: Carol
authors.initials: C
authors.lastname: Lakkis
authors.affiliation: Johnson & Johnson Vision Care, Inc, Jacksonville, Florida.
authors.forename: Lauren
authors.initials: L
authors.lastname: May
authors.affiliation: Johnson & Johnson Vision Care, Inc, Jacksonville, Florida.
authors.forename: Randall B
authors.initials: RB
authors.lastname: Pugh
mesh_terms: D000048:Acanthamoeba / Q000302:isolation & purification / Q000528:radiation effects*; D000818:Animals; D003261:Contact Lenses / Q000469:parasitology*; D004202:Disinfectants; D004203:Disinfection / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D011446:Prospective Studies; D014466:Ultraviolet Rays
chemical_list: D004202:Disinfectants