title: Determination of 4-hydroxy-2-nonenal in serum by high-performance liquid chromatography with fluorescence detection after pre-column derivatization using 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2, 1,3-benzoxadiazole.
abstract: 4-Hydroxy-2-nonenal (4HNE) is a major aldehyde generated during lipid peroxidation. The clinical monitoring of 4HNE in biological fluids should be useful for the early diagnosis of several diseases involving lipid peroxidation, such as rheumatoid arthritis, Parkinson's disease and cancer. In this study, an HPLC with fluorescence detection method was developed for the determination of 4HNE in human serum. The proposed method involves the extraction of 4HNE from human serum by sub-zero temperature extraction and fluorescent labeling of 4HNE with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2, 1,3-benzoxadiazole. The lower detection limit (signal-to-noise ratio = 3) of the method was 0.06 μm in serum. The proposed method was successfully applied to the measurement of 4HNE in sera obtained from patients with rheumatoid arthritis.
authors.affiliation: Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan; Sasebo Chuo Hospital, Sasebo, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Imazato
authors.forename: Akina
authors.initials: A
authors.lastname: Shiokawa
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kurose
authors.forename: Yasuha
authors.initials: Y
authors.lastname: Katou
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.forename: Kaname
authors.initials: K
authors.lastname: Ohyama
authors.forename: Marwa Fathy Bakr
authors.initials: MF
authors.lastname: Ali
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Ueki
authors.forename: Eisuke
authors.initials: E
authors.lastname: Maehata
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
keywords: 4-hydroxy-2-nonenal (4HNE); fluorescent labeling; rheumatoid arthritis; sub-zero temperature extraction