title: Evaluation of a molecularly imprinted polymer for determination of steroids in goat milk by matrix solid phase dispersion.
abstract: A molecularly imprinted polymer-matrix solid-phase dispersion methodology for simultaneous determination of five steroids in goat milk samples was proposed. Factors affecting the extraction recovery such as sample/dispersant ratio and washing and elution solvents were investigated. The molecularly imprinted polymer used as dispersant in the matrix solid-phase dispersion procedure showed high affinity to steroids, and the obtained extracts were sufficiently cleaned to be directly analyzed. Analytical separation was performed by micellar electrokinetic chromatography using a capillary electrophoresis system equipped with a diode array detector. A background electrolyte composed of borate buffer (25mM, pH 9.3), sodium dodecyl sulfate (10mM) and acetonitrile (20%) was used. The developed MIP-MSPD methodology was applied for direct determination of testosterone (T), estrone (E1), 17β-estradiol (17β-E2), 17α-ethinylestradiol (EE2) and progesterone (P) in different goat milk samples. Mean recoveries obtained ranged from 81% to 110%, with relative standard deviations (RSD)≤12%. The molecularly imprinted polymer-matrix solid-phase dispersion method is fast, selective, cost-effective and environment-friendly compared with other pretreatment methods used for extraction of steroids in milk.
authors.affiliation: Departamento de Química Inorgánica y Analítica, E.S.C.E.T, Universidad Rey Juan Carlos, C/Tulipán s/n, Móstoles, 28933 Madrid, Spain.
authors.forename: Judith
authors.initials: J
authors.lastname: Gañán
authors.affiliation: Departamento de Química Inorgánica y Analítica, E.S.C.E.T, Universidad Rey Juan Carlos, C/Tulipán s/n, Móstoles, 28933 Madrid, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Morante-Zarcero
authors.affiliation: Departamento de Ciencias Analíticas, Facultad de Ciencias, Universidad Nacional de Educación a Distancia (UNED), Senda del Rey, n° 9, 28040 Madrid, Spain.
authors.forename: Alejandrina
authors.initials: A
authors.lastname: Gallego-Picó
authors.affiliation: Departamento de Ciencias Analíticas, Facultad de Ciencias, Universidad Nacional de Educación a Distancia (UNED), Senda del Rey, n° 9, 28040 Madrid, Spain.
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Garcinuño
authors.affiliation: Departamento de Ciencias Analíticas, Facultad de Ciencias, Universidad Nacional de Educación a Distancia (UNED), Senda del Rey, n° 9, 28040 Madrid, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Fernández-Hernando
authors.affiliation: Departamento de Química Inorgánica y Analítica, E.S.C.E.T, Universidad Rey Juan Carlos, C/Tulipán s/n, Móstoles, 28933 Madrid, Spain. Electronic address: isabel.sierra@urjc.es.
authors.forename: Isabel
authors.initials: I
authors.lastname: Sierra
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D001881:Borates / Q000737:chemistry; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary; D004958:Estradiol / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004970:Estrone / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004997:Ethinyl Estradiol / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D006041:Goats; D008892:Milk / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D011247:Pregnancy; D011374:Progesterone / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D013256:Steroids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D013739:Testosterone / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000097:Acetonitriles; D001881:Borates; D002021:Buffers; D011108:Polymers; D013256:Steroids; D004970:Estrone; D012967:Sodium Dodecyl Sulfate; D013739:Testosterone; D004997:Ethinyl Estradiol; D011374:Progesterone; D004958:Estradiol; C032159:acetonitrile
keywords: Goat milk; MEKC; Matrix solid-phase dispersion; Molecularly imprinted polymer; Steroids