title: Protective effect of resveratrol on sodium fluoride-induced oxidative stress, hepatotoxicity and neurotoxicity in rats.
abstract: Protective effect of resveratrol on sodium fluoride-induced oxidative stress, hepatotoxicity and neurotoxicity were studied in rats. A total of 28 Wistar albino male rats were used. Four study groups were randomly formed with seven animals in each. The groups were treated for 21days with distilled water (control group), with water containing 100ppm fluoride (fluoride group), with resveratrol (12.5mg/kg i.p., resveratrol group), or with 100ppm fluoride+12.5mg/kg resveratrol i.p. (fluoride+resveratrol group). At the end of the trial, blood samples were collected by cardiac puncture and tissue samples were taken simultaneously. The total antioxidant and oxidant status in plasma and tissues as well as plasma 8-hydroxydeoxyguanosine levels were measured. Histopathological analyses of rat liver and brain tissues were performed in all groups to identify any changes. In the fluoride group, the total oxidant levels increased in plasma, liver and brain and total antioxidant levels decreased, as did the plasma 8-hydroxy-deoxyguanosine levels. These changes were prevented by co-administration of resveratrol. In addition, fluoride-associated severe histopathological changes in brain and liver tissues were not observed in the fluoride+resveratrol group. Consequently, these data suggested that resveratrol had beneficial effects in alleviating fluoride-induced oxidative stress.
authors.affiliation: Kirikkale University, Faculty of Veterinary Medicine, Department of Physiology, Kirikkale, Turkey. Electronic address: nurgulzengin@yahoo.com.
authors.forename: Nurgul
authors.initials: N
authors.lastname: Atmaca
authors.affiliation: Kirikkale University, Faculty of Veterinary Medicine, Department of Pathology, Kirikkale, Turkey. Electronic address: ht_atmaca@yahoo.com.
authors.forename: Hasan Tarik
authors.initials: HT
authors.lastname: Atmaca
authors.affiliation: Kafkas University, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Kars, Turkey. Electronic address: akanici@hotmail.com.
authors.forename: Ayse
authors.initials: A
authors.lastname: Kanici
authors.affiliation: Kirikkale University, Faculty of Veterinary Medicine, Department of Pathology, Kirikkale, Turkey. Electronic address: tugceanteplioglu@gmail.com.
authors.forename: Tugce
authors.initials: T
authors.lastname: Anteplioglu
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001921:Brain / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D006321:Heart / Q000187:drug effects; D008099:Liver / Q000187:drug effects*; D008297:Male; D020258:Neurotoxicity Syndromes / Q000188:drug therapy*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000009:adverse effects*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D000080242:8-Hydroxy-2'-Deoxyguanosine; D012969:Sodium Fluoride; D003849:Deoxyguanosine; D000077185:Resveratrol
keywords: Brain; Fluoride toxicity; Liver; Oxidative stress; Rats; Resveratrol