title: Altered immunohistochemical expression of mast cell tryptase and chymase in the pathogenesis of oral submucous fibrosis and malignant transformation of the overlying epithelium.
abstract: Mast cells (MCs) expressing serine proteases; tryptase and chymase, are associated with fibrosis in various diseases. However, little is known about their involvement in oral submucous fibrosis (OSF). Our goal was to evaluate the role of MC tryptase and chymase in the pathogenesis of OSF and its malignant transformation. Immunohistochemical expression of MC tryptase and chymase was evaluated in 20 cases of OSF, 10 cases of oral squamous cell carcinoma (OSCC) and 10 cases of healthy controls. Subepithelial zone of Stage 1 and 2 while deep zone of Stage 3 and 4 OSF demonstrated increased tryptase positive MCs. OSCC revealed a proportionate increase in tryptase and chymase positive MCs irrespective of areas of distribution. An altered balance in the subepithelial and deep distribution of tryptase and chymase positive MCs play an important role in the pathogenesis of OSF and its malignant transformation.
authors.affiliation: Department of Oral Pathology, Nair Hospital Dental College, Mumbai, India.
authors.forename: Archana
authors.initials: A
authors.lastname: Yadav
authors.affiliation: Department of Oral Pathology, Nair Hospital Dental College, Mumbai, India.
authors.forename: Rajiv S
authors.initials: RS
authors.lastname: Desai
authors.affiliation: Department of Oral Pathology, Nair Hospital Dental College, Mumbai, India.
authors.forename: Bansari A
authors.initials: BA
authors.lastname: Bhuta
authors.affiliation: Department of Oral Pathology, Nair Hospital Dental College, Mumbai, India.
authors.forename: Jatinder S
authors.initials: JS
authors.lastname: Singh
authors.affiliation: Department of Oral Pathology, Nair Hospital Dental College, Mumbai, India.
authors.forename: Reema
authors.initials: R
authors.lastname: Mehta
authors.affiliation: Department of Oral Pathology, Nair Hospital Dental College, Mumbai, India.
authors.forename: Akash P
authors.initials: AP
authors.lastname: Nehete
mesh_terms: D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D053818:Chymases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology; D009914:Oral Submucous Fibrosis / Q000209:etiology* / Q000473:pathology; D053802:Tryptases / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D053818:Chymases; D053802:Tryptases