title: Zearalenone degradation by two Pseudomonas strains from soil.
abstract: This study describes the screening of soil bacteria for their capability to degrade zearalenone (ZEN), employing an enrichment technique in which ZEN is used as the sole carbon source. Two isolates that were able to degrade ZEN belonged to the genus Pseudomonas according to biochemical characterization and 16S rRNA gene sequence and were named as Pseudomonas alcaliphila TH-C1 and Pseudomonas plecoglossicida TH-L1, respectively. The results showed that the degradation rates of P. alcaliphila TH-C1 and P. plecoglossicida TH-L1 for ZEN (2 μg/ml) were 68 ± 0.85 % and 57 ± 0.73%, when incubated for 72 h at 30 °C in a rotary shaker (160 rpm) and detected by high-performance liquid chromatograms (HPLC). These results suggest that the two Pseudomonas strains are new bacterial resource for degrading ZEN and can provide a new approach for the detoxification of ZEN.
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, 625014, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tan
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Hu
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Fei
authors.initials: F
authors.lastname: Liao
authors.forename: Biao
authors.initials: B
authors.lastname: Luo
authors.forename: Yajun
authors.initials: Y
authors.lastname: He
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ren
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhong
authors.forename: Guangneng
authors.initials: G
authors.lastname: Peng
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
mesh_terms: D015373:Bacterial Typing Techniques; D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature; D015025:Zearalenone / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D015025:Zearalenone; D002244:Carbon