title: Myeloperoxidase gene polymorphism predicts fibrosis severity in women with hepatitis C.
abstract: Oxidative stress plays an important role on liver fibrosis progression in the course of hepatitis C virus (HCV) infection. Myeloperoxidase (MPO) is an enzyme released by neutrophils and macrophages, responsible for generating hypochlorous acid and reactive oxygen species (ROS) that may lead to liver injury in HCV infection. On the other hand, antioxidant enzymes such as manganese superoxide dismutase (SOD) controls ROS-mediated damage. The aim of the present study was to investigate the influence of MPO G-463A and SOD2 Ala16Val polymorphisms in the severity of liver fibrosis in individuals with chronic HCV infection. The present study included 270 patients with chronic HCV recruited from the Gastrohepatology Service of the Oswaldo Cruz University Hospital/Liver Institute of Pernambuco (Recife, Northeastern Brazil). All patients underwent liver biopsy, which was classified according METAVIR score. The SNPs were determined by real-time PCR. After multivariate analysis adjustment, the GG genotype of MPO and the presence of metabolic syndrome were independently associated with fibrosis severity in women (P = 0.025 OR 2.25 CI 1.10-4.59 and P = 0.032 OR 2.32 CI 1.07-5.01, respectively). The presence of the GG genotype seems to be a risk factor for fibrosis severity in women with HCV.
authors.affiliation: Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco, Pernambuco, Brazil; Rede Nordeste de Biotecnologia, Pernambuco, Brazil. Electronic address: rodrigo.carmo@univasf.edu.br.
authors.forename: Rodrigo Feliciano
authors.initials: RF
authors.lastname: do Carmo
authors.affiliation: Instituto do Fígado de Pernambuco, Pernambuco, Brazil.
authors.forename: Luydson Richardson Silva
authors.initials: LR
authors.lastname: Vasconcelos
authors.affiliation: Rede Nordeste de Biotecnologia, Pernambuco, Brazil.
authors.forename: Taciana Furtado
authors.initials: TF
authors.lastname: Mendonça
authors.affiliation: Instituto de Ciências Biológicas, Universidade de Pernambuco, Pernambuco, Brazil.
authors.forename: Maria do Socorro
authors.initials: Mdo S
authors.lastname: de Mendonça Cavalcanti
authors.affiliation: Instituto do Fígado de Pernambuco, Pernambuco, Brazil; Faculdade de Ciências Médias, Universidade de Pernambuco, Pernambuco, Brazil.
authors.forename: Leila Maria Moreira Beltrão
authors.initials: LM
authors.lastname: Pereira
authors.affiliation: Instituto de Ciências Biológicas, Universidade de Pernambuco, Pernambuco, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Moura
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000502:physiology; D019698:Hepatitis C, Chronic / Q000150:complications / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D008103:Liver Cirrhosis / Q000201:enzymology / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D009195:Peroxidase / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012720:Severity of Illness Index; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
chemical_list: D009195:Peroxidase; D013482:Superoxide Dismutase
keywords: Fibrosis; HCV; MPO; Polymorphism; SOD