title: Biosensing platform based on graphene oxide via self-assembly induced by synergic interactions.
abstract: A novel self-assembled glucose biosensor based on graphene oxide (GO) was constructed by using 1-pyrenebutyric acid-N-hydroxysuccinimide ester (PANHS) as linking molecular. The stepwise self-assembly process was performed for PANHS anchoring in N,N-dimethylformamide (DMF) solvent and the further glucose oxidase (GOD) binding in aqueous solution, respectively. The molecular interactions and the morphologic properties were characterized by Fourier transform infrared spectroscopy (FTIR), field emission scanning electronic microscopy (FESEM), and atomic force microscopy (AFM). In addition, the quantitative loadings of anchored PANHS and GOD were well elucidated by surface plasmon resonance (SPR) measurements. The obtained novel glucose sensor exhibited satisfactory analytical performance to glucose: wide linear range (4.0×10(-6) to 4.4×10(-3) M), fast response (10s), high sensitivity (40.5±0.4 mA M(-1) cm(-2)), and low detection limit (2 μM, S/N=3). Furthermore, the biosensor exhibited excellent long-term stability and satisfactory reproducibility.
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Key Laboratory of Environmental Materials & Environmental Engineering of Jiangsu Province, School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China; Department of Energy and Resources, Shuozhou Vocational and Technical College, Shouzhou 036002, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Tian
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Pei-Xin
authors.initials: PX
authors.lastname: Yuan
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Key Laboratory of Environmental Materials & Environmental Engineering of Jiangsu Province, School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China. Electronic address: danshan@njust.edu.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, China.
authors.forename: Shou-Nian
authors.initials: SN
authors.lastname: Ding
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Guang-Yao
authors.initials: GY
authors.lastname: Zhang
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Xue-Ji
authors.initials: XJ
authors.lastname: Zhang
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D004126:Dimethylformamide / Q000737:chemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D004800:Enzymes, Immobilized; D010087:Oxides; D011721:Pyrenes; D012997:Solvents; D013388:Succinimides; C002218:1-pyrenebutyrate; D010984:Platinum; D006108:Graphite; D004126:Dimethylformamide; D005949:Glucose Oxidase; D005947:Glucose; C001426:N-hydroxysuccinimide
keywords: Biosensor; Graphene oxide; Pyrenebutyric acid–N-hydroxysuccinimide ester (PANHS); Self-assembly