title: Studies on the interaction between 9-fluorenylmethyl chloroformate and Fe3+ and Cu2+ ions: spectroscopic and theoretical calculation approach.
abstract: The interaction between 9-fluorenylmethyl chloroformate (FMOC-Cl) and Fe3+ and Cu2+ ions was investigated using fluorescence, UV/Vis absorption spectroscopies and theoretical calculation. The optical property of FMOC-Cl was studied in detail in absence and presence of various transition metal ions with particular affinity to Fe3+ and Cu2+ ions. With the fluorescence characteristic band centered at 307 and 315 nm for FMOC-Cl, the introduction of Fe3+ or Cu2+ ions leads to the fluorescence quenching of FMOC-Cl with different shift and intensities of two fluorescent bands. It allows us to differentiate between FMOC-Cl and Fe3+ and Cu2+ ions interaction behavior. The study on fluorescent kinetics confirms that the fluorescence quenching of FMOC-Cl with Fe3+ and Cu2+ ions is based on the formation of non-fluorescent material, that is, static quenching. Further analyses of bond lengths, Mulliken atomic charges and the frontier orbital compositions for FMOC-Cl and its complexes with Fe3+ and Cu2+ ions were carried out. The theoretical calculations prove the fluorescence quenching originates from the formation of coordination bonds between the oxygen atom of the carbonyl group of FMOC-Cl and Fe3+ and Cu2+ ions. The commercially available FMOC-Cl can be used as excellent fluorescent probe toward Fe3+ and Cu2+ ions with high sensitivity.
authors.affiliation: School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Zhenyan
authors.initials: Z
authors.lastname: Gu
authors.affiliation: School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Lei
authors.affiliation: School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Shi
authors.affiliation: School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: haoqingli@yahoo.com.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Hao
authors.affiliation: School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Weimeng
authors.initials: W
authors.lastname: Si
authors.affiliation: School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Xia
authors.affiliation: School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Wang
mesh_terms: D003300:Copper / Q000737:chemistry*; D005449:Fluorenes / Q000737:chemistry*; D007477:Ions; D007501:Iron / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005449:Fluorenes; D007477:Ions; D003300:Copper; C054007:1-(9-fluorenyl)methyl chloroformate; D007501:Iron
keywords: 9-Fluorenylmethyl chloroformate; Fluorescence spectroscopy; Optical analysis; Quantum chemical calculation; Static quenching