title: Simultaneous measurement of position and color of single fluorescent emitters using diffractive optics.
abstract: We propose a method for simultaneously measuring the position and emission color of single fluorescent emitters based on the use of a large pitch diffraction grating in the emission light path. The grating produces satellite spots adjacent to the main spot; the relative distance between the spots is a measure for the emission wavelength. We present proof-of-principle experiments on beads and mixtures of quantum dots using a spatial light modulator for making a programmable diffraction grating. A wavelength precision of around 10 nm can be achieved for 1000 signal photons and practical background levels, while maintaining a localization precision of around 10 nm.
authors.forename: Jordi
authors.initials: J
authors.lastname: Broeken
authors.forename: Bernd
authors.initials: B
authors.lastname: Rieger
authors.forename: Sjoerd
authors.initials: S
authors.lastname: Stallinga
mesh_terms: D003116:Color; D005456:Fluorescent Dyes; D055620:Optical Phenomena; D017785:Photons; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes