title: RNA-guided genome editing in Drosophila with the purified Cas9 protein.
abstract: We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
authors.affiliation: Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea Functional Genomics Department, Korea University of Science and Technology (KUST), Daejeon 305-333, Korea.
authors.forename: Jeong-Soo
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kwak
authors.affiliation: National Creative Research Initiatives Center for Genome Engineering and Department of Chemistry, Seoul National University, Seoul 151-747, Korea kweonyu@kribb.re.kr jskim01@snu.ac.kr.
authors.forename: Jungeun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea.
authors.forename: Ae-Kyeong
authors.initials: AK
authors.lastname: Kim
authors.affiliation: Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea.
authors.forename: Hae Min
authors.initials: HM
authors.lastname: Noh
authors.affiliation: National Creative Research Initiatives Center for Genome Engineering and Department of Chemistry, Seoul National University, Seoul 151-747, Korea Center for Genome Engineering, Institute for Basic Science, Daejeon, Korea kweonyu@kribb.re.kr jskim01@snu.ac.kr.
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea Functional Genomics Department, Korea University of Science and Technology (KUST), Daejeon 305-333, Korea kweonyu@kribb.re.kr jskim01@snu.ac.kr.
authors.forename: Kweon
authors.initials: K
authors.lastname: Yu
mesh_terms: D000818:Animals; D001483:Base Sequence; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D016678:Genome; D018095:Germ-Line Mutation; D017393:RNA Editing; D000094704:RNA, Guide, CRISPR-Cas Systems
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D000094704:RNA, Guide, CRISPR-Cas Systems; C116191:e protein, Drosophila
keywords: Cas9 protein; Drosophila; RGEN