title: Monitoring enzymatic ATP hydrolysis by EPR spectroscopy.
abstract: An adenosine triphosphate (ATP) analogue modified with two nitroxide radicals is developed and employed to study its enzymatic hydrolysis by electron paramagnetic resonance spectroscopy. For this application, we demonstrate that EPR holds the potential to complement fluorogenic substrate analogues in monitoring enzymatic activity.
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany. malte.drescher@uni-konstanz.de andreas.marx@uni-konstanz.de stephan.hacker@uni-konstanz.de.
authors.forename: Stephan M
authors.initials: SM
authors.lastname: Hacker
authors.forename: Christian
authors.initials: C
authors.lastname: Hintze
authors.forename: Andreas
authors.initials: A
authors.lastname: Marx
authors.forename: Malte
authors.initials: M
authors.lastname: Drescher
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D006868:Hydrolysis
chemical_list: D000255:Adenosine Triphosphate