title: Identification of a Csr system in Serratia marcescens 2170.
abstract: The carbon storage regulator (Csr) global regulatory system is conserved in many eubacteria and coordinates the expression of various genes that facilitate adaptation during the major physiological growth phase. The Csr system in Escherichia coli comprises an RNA-binding protein, CsrA; small non-coding RNAs, CsrB and CsrC; and a decay factor for small RNAs, CsrD. In this study, we identified the Csr system in Serratia marcescens 2170. S. marcescens CsrA was 97% identical to E. coli CsrA. CsrB and CsrC RNAs had typical stem-loop structures, including a GGA motif that is the CsrA binding site. CsrD was composed of N-terminal two times transmembrane region and HAMP-like, GGDEF, and EAL domains. Overexpression of S. marcescens csr genes complemented the phenotype of E. coli csr mutants. S. marcescens CsrD affected the decay of CsrB and CsrC RNAs in E. coli. These results suggest that the Csr system in S. marcescens is composed of an RNA-binding protein, two Csr small RNAs, and a decay factor for Csr small RNAs.
authors.affiliation: Graduate School of Science and Technology, Niigata University.
authors.forename: Manabu
authors.initials: M
authors.lastname: Ito
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nomura
authors.forename: Hayuki
authors.initials: H
authors.lastname: Sugimoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Kazushi
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000222:Adaptation, Physiological; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020871:RNA Stability; D058727:RNA, Small Untranslated / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012706:Serratia marcescens / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D058727:RNA, Small Untranslated; D014157:Transcription Factors; D002244:Carbon