title: Characterisation of cytoplasmic DNA complementary to non-retroviral RNA viruses in human cells.
abstract: The synthesis and subsequent genomic integration of DNA that is complementary to the genomes of non-retroviral RNA viruses are rarely observed. However, upon infection of various human cell lines and primary fibroblasts with the vesicular stomatitis virus (VSV), we detected DNA complementary to the VSV RNA. The VSV DNA was detected in the cytoplasm as single-stranded DNA fully complementary to the viral mRNA from the poly(A) region to the 7-methyl guanosine cap. The formation of this DNA was cell-dependent. Experimentally, we found that the transduction of cells that do not produce VSV DNA with the long interspersed nuclear element 1 and their infection with VSV could lead to the formation of VSV DNA. Viral DNA complementary to other RNA viruses was also detected in the respective infected human cells. Thus, the genetic information of the non-retroviral RNA virus genome can flow into the DNA of mammalian cells expressing LINE-1-like elements.
authors.affiliation: 1] Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, JAPAN [2] Department of Dermatology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, JAPAN [3] MRC/UCL Centre for Medical Molecular Virology and Wohl Virion Centre, Division of Infection and Immunity, University College London, Gower Street, London WC1E 6BT, United Kingdom.
authors.forename: Akira
authors.initials: A
authors.lastname: Shimizu
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, JAPAN.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakatani
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, JAPAN.
authors.forename: Takako
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, JAPAN.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Jinno-Oue
authors.affiliation: Department of Dermatology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, JAPAN.
authors.forename: Osamu
authors.initials: O
authors.lastname: Ishikawa
authors.affiliation: High Throughput Biology Centre, Johns Hopkins University School of Medicine, 733 N. Broadway/335 Broadway Research Building, Baltimore, Maryland, 21205, USA.
authors.forename: Jef D
authors.initials: JD
authors.lastname: Boeke
authors.affiliation: MRC/UCL Centre for Medical Molecular Virology and Wohl Virion Centre, Division of Infection and Immunity, University College London, Gower Street, London WC1E 6BT, United Kingdom.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takeuchi
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, JAPAN.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Hoshino
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D003593:Cytoplasm; D018076:DNA, Complementary / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005347:Fibroblasts / Q000502:physiology* / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D012190:Retroviridae / Q000235:genetics; D054243:Vesicular Stomatitis / Q000235:genetics*; D016662:Virus Integration / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral