title: Synaptotagmin 11 interacts with components of the RNA-induced silencing complex RISC in clonal pancreatic β-cells.
abstract: Synaptotagmins are two C2 domain-containing transmembrane proteins. The function of calcium-sensitive members in the regulation of post-Golgi traffic has been well established whereas little is known about the calcium-insensitive isoforms constituting half of the protein family. Novel binding partners of synaptotagmin 11 were identified in β-cells. A number of them had been assigned previously to ER/Golgi derived-vesicles or linked to RNA synthesis, translation and processing. Whereas the C2A domain interacted with the Q-SNARE Vti1a, the C2B domain of syt11 interacted with the SND1, Ago2 and FMRP, components of the RNA-induced silencing complex (RISC). Binding to SND was direct via its N-terminal tandem repeats. Our data indicate that syt11 may provide a link between gene regulation by microRNAs and membrane traffic.
authors.affiliation: Université de Bordeaux, UMR CNRS 5248, F-33607 Pessac, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Milochau
authors.affiliation: Université de Bordeaux, UMR CNRS 5248, F-33607 Pessac, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Lagrée
authors.affiliation: Université de Bordeaux, UMR CNRS 5248, F-33607 Pessac, France.
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Benassy
authors.affiliation: Université de Bordeaux, UMR CNRS 5248, F-33607 Pessac, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Chaignepain
authors.affiliation: Université de Bordeaux, UMR CNRS 5248, F-33607 Pessac, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Papin
authors.affiliation: University of the Basque Country, Faculty of Medicine and Dentistry, Department of Physiology, Bilbao, Spain.
authors.forename: Itsaso
authors.initials: I
authors.lastname: Garcia-Arcos
authors.affiliation: Université Montpellier 1, CNRS FRE 3400, Faculté de Pharmacie, F-34093 Montpellier Cedex 5, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Lajoix
authors.affiliation: Université de Bordeaux, UMR CNRS 5248, F-33607 Pessac, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Monterrat
authors.affiliation: Université de Bordeaux, UMR CNRS 5248, F-33607 Pessac, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Coudert
authors.affiliation: Université de Bordeaux, UMR CNRS 5248, F-33607 Pessac, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Schmitter
authors.affiliation: University of the Basque Country, Faculty of Medicine and Dentistry, Department of Physiology, Bilbao, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Ochoa
authors.affiliation: Université de Bordeaux, UMR CNRS 5248, F-33607 Pessac, France. Electronic address: j.lang@cbmn.u-bordeaux.fr.
authors.forename: Jochen
authors.initials: J
authors.lastname: Lang
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000378:metabolism; D002460:Cell Line; D004720:Endonucleases; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000502:physiology; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D021381:Protein Transport; D034622:RNA Interference; D034743:RNA-Induced Silencing Complex / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D050857:Synaptotagmins / Q000378:metabolism*
chemical_list: D060565:Argonaute Proteins; D004720:Endonucleases; D051860:Fragile X Messenger Ribonucleoprotein 1; D035683:MicroRNAs; D009687:Nuclear Proteins; D034743:RNA-Induced Silencing Complex; D050857:Synaptotagmins; C526735:Ago2 protein, mouse; C497684:Fmr1 protein, mouse; C494266:Syt11 protein, mouse; C490640:Snd1 protein, mouse
keywords: ER-Golgi; Pancreatic Islets; RISC; SNARE; Secretory pathway; Synaptotagmins