title: Laser Desorption/Ionization Mass Spectrometry (LDI-MS) of Lipids with Iron Oxide Nanoparticle-Coated Targets.
abstract: Iron oxide nanoparticle (NP)-coated target plates were employed for the direct detection and analysis of low molecular weight lipids by laser desorption/ionization (LDI) mass spectrometry (MS). We have demonstrated that the use of the iron oxide NP-coated target provides a simple, direct, and rapid detection method for lipid standards and epidermal surface lipids without any cumbersome sample pretreatment as well as mass spectra that are free of background matrix peaks. Lipid standards (1-stearoyl-sn-glycero-3-phosphocholine, 1,2-dioleoyl-sn-glycerol, 1-palmitoyl-2-oleoyl-3-linoleoyl-rac-glycerol, 1,2-distearoyl-sn-glycero-3-phosphocholine) were detected as either protonated or cationated species. Clean MS/MS spectra for each lipid were also successfully obtained. Pre-MS surface cleaning of the target plates with UV-ozone treatment successfully removed organic contaminants that would interfere with the mass spectra especially in the low molecular weight region. Preliminary application of the presented target plate to the detection of endogenous lipids in latent fingerprints showed promising results and for potential use in the visualization and chemical composition determination of latent fingerprints by nanoparticle assistance.
authors.affiliation: Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation.
authors.forename: Maiko
authors.initials: M
authors.lastname: Kusano
authors.affiliation: Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation.
authors.forename: Shin-Ichirou
authors.initials: S
authors.lastname: Kawabata
authors.affiliation: Dai Nippon Toryo Co., Ltd.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tamura
authors.affiliation: Dai Nippon Toryo Co., Ltd.
authors.forename: Daigou
authors.initials: D
authors.lastname: Mizoguchi
authors.affiliation: Dai Nippon Toryo Co., Ltd.
authors.forename: Masato
authors.initials: M
authors.lastname: Murouchi
authors.affiliation: Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials, and Bioengineering, Kansai University.
authors.forename: Hideya
authors.initials: H
authors.lastname: Kawasaki
authors.affiliation: Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials, and Bioengineering, Kansai University.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Arakawa
authors.affiliation: Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanaka
keywords: MALDI; SALDI; iron oxide nanoparticle; laser desorption/ionization; lipids; mass spectrometry