title: Clonal Mature Adipocyte Production of Proliferative-competent Daughter Cells Requires Lipid Export Prior to Cell Division.
abstract: OBJECTIVE
  Numerous in vitro observations have been published to show that mature adipocytes may resume proliferation and begin to populate the adipofibroblast fraction or form other cell types.
  
  
  RESULTS
  In the present study, we evaluated clonal cultures of mature pig-derived adipocytes as they began to reestablish their ability to divide. The lipid contained within the cytoplasm was either moved to the apical ends of the cell, or large droplets were physically extruded from the cell. In the latter case, we ascertained that the cell lipid droplet was handled in a different manner to that by beef-derived adipocytes as described in other published studies.
  
  
  CONCLUSIONS
  Pig-derived adipocytes expel large amounts of lipid directly into the medium environment prior to becoming capable of cell division, rather than retaining all lipids like the beef cells. This difference in lipid handling and trafficking may be a novel mechanism in adipocyte resumption of proliferation.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China ; Department of Animal Science, Washington State University, Pullman, WA 99164.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Departamento de Producción Agraria, Universidad Pública de Navarra, Pamplona 31006 Navarra, Spain.
authors.forename: Maitea
authors.initials: M
authors.lastname: Guridi
authors.affiliation: The Hartz Mountain Corporation, Bloomfield, NJ 07003.
authors.forename: Melinda E
authors.initials: ME
authors.lastname: Fernyhough
authors.affiliation: Department of Animal Science, Washington State University, Pullman, WA 99164.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada T6G 2P5.
authors.forename: LeLuo
authors.initials: L
authors.lastname: Guan
authors.affiliation: USDA-ARS, Richard B. Russell Agricultural Research Station, Athens, GA, 30604.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Hausman
authors.affiliation: Department of Animal Science, Washington State University, Pullman, WA 99164.
authors.forename: Michael V
authors.initials: MV
authors.lastname: Dodson
keywords: Adipocyte; Adipofibroblast; Dedifferentiation; Lipid mobilization; Pig; Proliferation