title: Routine sample preparation and HPLC analysis for ascorbic acid (vitamin C) determination in wheat plants and Arabidopsis leaf tissues.
abstract: Plants have developed various mechanisms to protect themselves against oxidative stress. One of the most important non-enzymatic antioxidants is ascorbic acid. There is thus a need for a rapid, sensitive method for the analysis of the reduced and oxidised forms of ascorbic acid in crop plants. In this paper a simple, economic, selective, precise and stable HPLC method is presented for the detection of ascorbate in plant tissue. The sensitivity, the short retention time and the simple isocratic elution mean that the method is suitable for the routine quantification of ascorbate in a high daily sample number. The method has been found to be better than previously reported methods, because of the use of an economical, readily available mobile phase, UV detection and the lack of complicated extraction procedures. The method has been tested on Arabidopsis plants with different ascorbate levels and on wheat plants during Cd stress.
authors.affiliation: Hungarian Academy of Sciences Department of Plant Physiology, Agricultural Institute, Centre for Agricultural Research Brunszvik u. 2 H-2462 Martonvásár Hungary.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Szalai
authors.affiliation: Hungarian Academy of Sciences Department of Plant Physiology, Agricultural Institute, Centre for Agricultural Research Brunszvik u. 2 H-2462 Martonvásár Hungary.
authors.forename: T
authors.initials: T
authors.lastname: Janda
authors.affiliation: Hungarian Academy of Sciences Department of Plant Physiology, Agricultural Institute, Centre for Agricultural Research Brunszvik u. 2 H-2462 Martonvásár Hungary.
authors.forename: Magda
authors.initials: M
authors.lastname: Pál
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism*; D019187:Cadmium Compounds / Q000494:pharmacology; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D005838:Genotype; D009566:Nitrates / Q000494:pharmacology; D010641:Phenotype; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D013312:Stress, Physiological; D014908:Triticum / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D029681:Arabidopsis Proteins; D019187:Cadmium Compounds; D009566:Nitrates; C522629:GDP-L-galactose phosphorylase, Arabidopsis; D010744:Phosphoric Monoester Hydrolases; D001205:Ascorbic Acid; C035196:cadmium nitrate
keywords: Ascorbic acid; HPLC analysis; UV detection; plant tissue; stress