title: Bordetella pertussis FbpA binds both unchelated iron and iron siderophore complexes.
abstract: Bordetella pertussis is the causative agent of whooping cough. This pathogenic bacterium can obtain the essential nutrient iron using its native alcaligin siderophore and by utilizing xeno-siderophores such as desferrioxamine B, ferrichrome, and enterobactin. Previous genome-wide expression profiling identified an iron repressible B. pertussis gene encoding a periplasmic protein (FbpABp). A previously reported crystal structure shows significant similarity between FbpABp and previously characterized bacterial iron binding proteins, and established its iron-binding ability. Bordetella growth studies determined that FbpABp was required for utilization of not only unchelated iron, but also utilization of iron bound to both native and xeno-siderophores. In this in vitro solution study, we quantified the binding of unchelated ferric iron to FbpABp in the presence of various anions and importantly, we demonstrated that FbpABp binds all the ferric siderophores tested (native and xeno) with μM affinity. In silico modeling augmented solution data. FbpABp was incapable of iron removal from ferric xeno-siderophores in vitro. However, when FbpABp was reacted with native ferric-alcaligin, it elicited a pronounced change in the iron coordination environment, which may signify an early step in FbpABp-mediated iron removal from the native siderophore. To our knowledge, this is the first time the periplasmic component of an iron uptake system has been shown to bind iron directly as Fe(3+) and indirectly as a ferric siderophore complex.
authors.affiliation: Department of Chemistry, Duke University , Durham, North Carolina 27708, United States.
authors.forename: Sambuddha
authors.initials: S
authors.lastname: Banerjee
authors.forename: Aruna J
authors.initials: AJ
authors.lastname: Weerasinghe
authors.forename: Claire J
authors.initials: CJ
authors.lastname: Parker Siburt
authors.forename: R Timothy
authors.initials: RT
authors.lastname: Kreulen
authors.forename: Sandra K
authors.initials: SK
authors.lastname: Armstrong
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Brickman
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Lambert
authors.forename: Alvin L
authors.initials: AL
authors.lastname: Crumbliss
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001886:Bordetella pertussis / Q000254:growth & development / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000378:metabolism; D033862:Iron-Binding Proteins / Q000378:metabolism*; D008958:Models, Molecular; D033902:Periplasmic Binding Proteins / Q000378:metabolism; D017262:Siderophores / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D005290:Ferric Compounds; D006877:Hydroxamic Acids; D033862:Iron-Binding Proteins; D033902:Periplasmic Binding Proteins; D017262:Siderophores; C092056:alcaligin