title: A novel quinoline molecular probe and the derived functionalized gold nanoparticles: sensing properties and cytotoxicity studies in MCF-7 human breast cancer cells.
abstract: A highly selective quinoline-based fluorescent sensor L was designed, prepared and used to monitor zinc ions in Goldfish (Carassius auratus) as model of vertebrate organism. Modified gold nanoparticles having functional quinoline molecules (GNPs@L) were also synthesized and their sensing properties towards different metal ions were also explored in solution, showing high selectively towards the toxic and heavy metal ion mercury. Cell proliferation kit XTT that employs 2,3-bis-(2-methoxy-4-nitro- 5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT) was used in order to investigate the cytotoxicity of compound L and GNPs@L on the MCF-7 breast cancer cells, showing significant cytotoxicity in comparison with similar reported systems. It was observed that L and GNPs@L compounds induced apoptosis in MCF-7 cancer cells. The cellular uptake of the hybrid system GNPs@L was studied using confocal laser scanning microscopy (CLSM).
authors.affiliation: REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Monte da Caparica, Portugal; Ecology Research Group, Department of Geographical and Life Sciences, Canterbury Christ Church University, CT1 1QU Canterbury, United Kingdom; Inorganic Chemistry Department, Faculty of Chemistry, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain. Electronic address: cristina.nunez@fct.unl.pt.
authors.forename: Cristina
authors.initials: C
authors.lastname: Núñez
authors.affiliation: REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Monte da Caparica, Portugal; ProteoMass Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal; Veterinary Science Department, (CECAV), University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal.
authors.forename: Elisabete
authors.initials: E
authors.lastname: Oliveira
authors.affiliation: Institut de Biotecnologia i de Biomedicina and Departament de Bioquímica i de Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: García-Pardo
authors.affiliation: REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Monte da Caparica, Portugal.
authors.forename: Mario
authors.initials: M
authors.lastname: Diniz
authors.affiliation: Institut de Biotecnologia i de Biomedicina and Departament de Bioquímica i de Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain. Electronic address: julia.lorenzo@uab.es.
authors.forename: Julia
authors.initials: J
authors.lastname: Lorenzo
authors.affiliation: REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Monte da Caparica, Portugal; ProteoMass Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Capelo
authors.affiliation: REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Monte da Caparica, Portugal; ProteoMass Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal. Electronic address: cle@fct.unl.pt.
authors.forename: Carlos
authors.initials: C
authors.lastname: Lodeiro
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006046:Gold / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D011804:Quinolines / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D011804:Quinolines; D006046:Gold; C037219:quinoline
keywords: Cytotoxicity; Fluorescent probes; Nanoparticles; Quinoline; XTT assay