title: Enamel matrix derivative promote primary human pulp cell differentiation and mineralization.
abstract: Enamel matrix derivative (EMD) has been found to induce reactive dentin formation; however the molecular mechanisms involved are unclear. The effect of EMD (5-50 μg/mL) on primary human pulp cells were compared to untreated cells and cells incubated with 10⁻⁸ M dexamethasone (DEX) for 1, 2, 3, 7, and 14 days in culture. Expression analysis using Affymetrix microchips demonstrated that 10 μg/mL EMD regulated several hundred genes and stimulated the gene expression of proteins involved in mesenchymal proliferation and differentiation. Both EMD and DEX enhanced the expression of amelogenin (amel), and the dentinogenic markers dentin sialophosphoprotein (DSSP) and dentin matrix acidic phosphoprotein 1 (DMP1), as well as the osteogenic markers osteocalcin (OC, BGLAP) and collagen type 1 (COL1A1). Whereas, only EMD had effect on alkaline phosphatase (ALP) mRNA expression, the stimulatory effect were verified by enhanced secretion of OC and COL1A from EMD treated cells, and increased ALP activity in cell culture medium after EMD treatment. Increased levels of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant proteins (MCP-1) in the cell culture medium were also found. Consequently, the suggested effect of EMD is to promote differentiation of pulp cells and increases the potential for pulpal mineralization to favor reactive dentine formation.
authors.affiliation: Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, Blindern, N-0317 Oslo, Norway. elisaba@odont.uio.no.
authors.forename: Elisabeth Aurstad
authors.initials: EA
authors.lastname: Riksen
authors.affiliation: Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, Blindern, N-0317 Oslo, Norway. maria.landin@odont.uio.no.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Landin
authors.affiliation: Department of Medical Biochemistry, Oslo University Hospital, N-0450 Oslo, Norway. sjur.reppe@medisin.uio.no.
authors.forename: Sjur
authors.initials: S
authors.lastname: Reppe
authors.affiliation: Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, Blindern, N-0317 Oslo, Norway. yukiomalmo@hotmail.com.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, Blindern, N-0317 Oslo, Norway. spl@odont.uio.no.
authors.forename: Ståle Petter
authors.initials: SP
authors.lastname: Lyngstadaas
authors.affiliation: Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, Blindern, N-0317 Oslo, Norway. j.e.reseland@odont.uio.no.
authors.forename: Janne E
authors.initials: JE
authors.lastname: Reseland
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003743:Dental Enamel / Q000378:metabolism; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D015675:Osteocalcin / Q000378:metabolism; D013552:Swine; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D016207:Cytokines; D003746:Dental Enamel Proteins; D015675:Osteocalcin; D003907:Dexamethasone