title: MicroRNA-206 suppresses gastric cancer cell growth and metastasis.
abstract: Gastric cancer is one of the leading causes of cancer death world-wide and carries a high rate of metastatic risk. In addition to other protein-coding oncogenes and tumor suppressor genes, microRNAs play an important role in gastric cancer tumorigenic progression. Here, we show that miR-206 is expressed at markedly low levels in a cohort of gastric tumors compared to their matching normal tissues, and in a number of gastric cancer cell lines. Down-regulation of miR-206 was particularly significant in tumors with lymphatic metastasis, local invasion, and advanced TNM staging. We find that forced expression of miR-206 suppressed the proliferation, colony-formation, and xenograft tumorigenesis of SCG-7901 cells, a line of gastric cancer cells. Forced expression of miR-206 also suppressed SCG-7901 cell migration and invasion, as well as metastasis in cell culture or tail-vein injected mouse models, respectively. The anti-metastatic effect of miR-206 is likely mediated by targeting metastasis regulatory genes STC2, HDAC4, KLF4, IGF1R, FRS2, SFRP1, BCL2, BDNF, and K-ras, which were drastically down-regulated by stable expression of exogenous miR-206 in SCG-7901 cells. Taken together, our results indicate that miR-206 is a tumor suppressor of gastric cancer acting at steps that regulate metastasis.
authors.affiliation: Department of General Surgery, Nanjing Medical University affiliated Changzhou No. 2 Hospital, 213000 Changzhou, Jiangsu, PR. China.|Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, 210029 Nanjing, Jiangsu, PR. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, 210029 Nanjing, Jiangsu, PR. China.
authors.forename: Hui-Jie
authors.initials: HJ
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Nanjing Medical University affiliated Changzhou No. 2 Hospital, 213000 Changzhou, Jiangsu, PR. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, 210029 Nanjing, Jiangsu, PR. China.
authors.forename: Shen
authors.initials: S
authors.lastname: Yue
authors.affiliation: Department of General Surgery, Nanjing Medical University affiliated Changzhou No. 2 Hospital, 213000 Changzhou, Jiangsu, PR. China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Tang
authors.affiliation: Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, 210029 Nanjing, Jiangsu, PR. China.
authors.forename: Steven Y
authors.initials: SY
authors.lastname: Cheng
keywords: Gastric cancer; Metastasis; Tumor suppressor; miR-206