title: Galectin fingerprinting in naso-sinusal diseases.
abstract: Galectins, a family of endogenous lectins, are multifunctional effectors that act at various sites and can be used in immunohistochemical localization studies of diseased states. Since they form a potentially cooperative and antagonistic network, we tested the hypothesis that histopathological fingerprinting of galectins could refine the molecular understanding of naso-sinusal pathologies. Using non-cross-reactive antibodies against galectin-1, -3, -4, -7, -8 and -9, we characterized the galectin profiles in chronic rhinosinusitis, nasal polyposis, inverted papillomas and squamous cell carcinomas. The expression, signal location and quantitative parameters describing the percentage of positive cells and labeling intensity were assessed for various cases. We discovered that inverted papillomas showed a distinct galectin immunohistochemical profile. Indeed, epithelial overexpression of galectin-3 (p=0.0002), galectin-4 (p<10-6), galectin-7 (p<10-6) and galectin-9 (p<10-6) was observed in inverted papillomas compared to non-malignant diseases. Regarding carcinomas, we observed increased expression of galectin-9 (p<10-6) in epithelial cells compared to non-tumor pathologies. Our results suggest that galectin-3, -4, -7 and -9 could be involved in the biology of inverted papillomas. In addition, we observed that the expression of galectin in naso-sinusal diseases seems to be affected by tumor progression and not inflammatory or allergic phenomena.
authors.affiliation: Laboratory of Anatomy, Faculty of Medicine and Pharmacy, University of Mons, Mons, Belgium.
authors.forename: Anaëlle
authors.initials: A
authors.lastname: Duray
authors.affiliation: Department of Oto-Rhino-Laryngology, CHU Saint-Pierre, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Thibault
authors.initials: T
authors.lastname: De Maesschalck
authors.affiliation: Laboratory of Image, Signal Processing and Acoustics (LISA), Ecole Polytechnique de Bruxelles, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Christine
authors.initials: C
authors.lastname: Decaestecker
authors.affiliation: Department of Pathology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Myriam
authors.initials: M
authors.lastname: Remmelink
authors.affiliation: Department of Oto-Rhino-Laryngology, CHU Saint-Pierre, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Chantrain
authors.affiliation: Laboratory of Anatomy, Faculty of Medicine and Pharmacy, University of Mons, Mons, Belgium.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Neiveyans
authors.affiliation: Department of Oto-Rhino-Laryngology, CHU Saint-Pierre, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Horoi
authors.affiliation: Department of Pathology, Faculty of Medicine, Hôpital Claude Huriez and Centre de Biologie-Pathologie, CHRU, Lille, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Leroy
authors.affiliation: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilian University of Munich, Munich, Germany.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Gabius
authors.affiliation: Laboratory of Anatomy, Faculty of Medicine and Pharmacy, University of Mons, Mons, Belgium.
authors.forename: Sven
authors.initials: S
authors.lastname: Saussez
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D002648:Child; D005260:Female; D037161:Galectins / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism / Q000473:pathology*; D018308:Papilloma, Inverted / Q000378:metabolism / Q000473:pathology; D010255:Paranasal Sinus Neoplasms / Q000378:metabolism / Q000473:pathology*; D012189:Retrospective Studies; D012220:Rhinitis / Q000378:metabolism / Q000473:pathology*; D012852:Sinusitis / Q000378:metabolism / Q000473:pathology*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D037161:Galectins