title: Up-regulation of CNDP2 facilitates the proliferation of colon cancer.
abstract: BACKGROUND
  Cytosolic nonspecific dipetidase (CN2) belongs to the family of M20 metallopeptidases. It was stated in previous articles that higher expression levels of CN2 were observed in renal cell carcinoma and breast cancer. Our study explored the correlation between CN2 and colon carcinogenesis.
  
  
  METHODS
  We analysed the relationship between 183 patients clinicopathological characteristics and its CN2 expression. To detect the levels of CN2 in colon cancer cell lines and colon cancer tissues by western blot. To verify cell proliferation in colon cancer cells with knockdown of CNDP2 and explore the causes of these phenomena.
  
  
  RESULTS
  The expression levels of CN2 in clinical colon tumors and colon cancer cell lines were significantly higher than that in normal colon mucosa and colon cell lines. The difference in CN2 levels was associated with tumor location (right- and left-sided colon cancer), but there was no significant association with age, gender, tumor size, tumor grade, tumor stage or serum carcinoembryonic antigen (CEA). Knockdown of CNDP2 inhibited cell proliferation, blocked cell cycle progression and retarded carcinogenesis in an animal model. The signaling pathway through which knockdown of CNDP2 inhibited cell proliferation and tumorigenesis involved in EGFR, cyclin B1 and cyclin E.
  
  
  CONCLUSIONS
  Knockdown of CNDP2 can inhibit the proliferation of colon cancer in vitro and retarded carcinogenesis in vivo.
authors.forename: Conglong
authors.initials: C
authors.lastname: Xue
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Zhang
authors.forename: Honglan
authors.initials: H
authors.lastname: Yu
authors.forename: Miao
authors.initials: M
authors.lastname: Yu
authors.forename: Kaitao
authors.initials: K
authors.lastname: Yuan
authors.forename: Ting
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan II Road, Guangzhou, Guangdong 510080, China. miaomy@163.com.
authors.forename: Mingyong
authors.initials: M
authors.lastname: Miao
authors.forename: Hanping
authors.initials: H
authors.lastname: Shi
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D000368:Aged; D000818:Animals; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism; D004150:Dipeptidases / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008819:Mice, Nude; D008875:Middle Aged; D014183:Transplantation, Heterologous; D015854:Up-Regulation
chemical_list: D004150:Dipeptidases; C031499:CNDP2 protein, human