title: Microarray-based resonance light scattering assay for detecting DNA methylation and human DNA methyltransferase simultaneously with high sensitivity.
abstract: A microarray-based resonance light scattering assay, with the combination of methylation-sensitive endonuclease and gold nanoparticle (GNP) probes, has been proposed to sensitively distinguish the DNA methylation level as low as 0.01% (10 pM methylated DNA in 100 nM total DNA) and detect human DNA methyltransferase 1 (Dnmt1) down to 0.1 U mL(-1).
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. wangzx@ciac.ac.cn.
authors.forename: Lan
authors.initials: L
authors.lastname: Ma
authors.forename: Min
authors.initials: M
authors.lastname: Su
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D004247:DNA / Q000032:analysis; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000032:analysis*; D019175:DNA Methylation; D057075:Enzyme Assays / Q000295:instrumentation; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006801:Humans; D008027:Light; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D046228:Microarray Analysis / Q000295:instrumentation*; D012542:Scattering, Radiation
chemical_list: D006046:Gold; D004247:DNA; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human