title: NGF and TERT co-transfected BMSCs improve the restoration of cognitive impairment in vascular dementia rats.
abstract: Vascular dementia (VaD) is a mental disorder caused by brain damage due to cerebrovascular disease, and incidence of VaD is rising. To date, there is no known effective cure for VaD, so effort in developing an effective treatment for VaD is of great importance. The differentiation plasticity of BMSCs, in conjunction with its weak immunogenicity, makes manipulated BMSCs an attractive strategy for disease treatment. However, BMSCs often display disabled differentiation, premature aging, and unstable proliferation, reducing their neuroprotective function. These problems may be caused by the lack of telomerase activity in BMSCs. Our results show that NGF-TERT co-transfected BMSCs have a better therapeutic effect than BMSCs lacking NGF and TERT expression, demonstrated by significant improvements in learning and memory in VaD rats. The underlying mechanism might be increased expression of NGF, TrkA and SYN in the hippocampal CA1 area, which has potential implication in advancing therapeutics for VaD.
authors.affiliation: Department of Neurology, General Hospital, Tianjin Medical University, Tianjin, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of clinical laboratory, General Hospital, Tianjin Medical University, Tianjin, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Chang
authors.affiliation: Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin, China; Tianjin Key Laboratory of Cellular and Molecular Immunology, Tianjin, China; Key Laboratory of Educational Ministry of China, Tianjin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Geng
mesh_terms: D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000378:metabolism*; D003072:Cognition Disorders / Q000209:etiology / Q000628:therapy*; D017931:DNA Primers; D015140:Dementia, Vascular / Q000150:complications*; D018380:Hematopoietic Stem Cell Transplantation; D020932:Nerve Growth Factor / Q000235:genetics*; D051381:Rats; D013234:Stem Cells / Q000378:metabolism*; D019098:Telomerase / Q000235:genetics*; D014162:Transfection
chemical_list: D017931:DNA Primers; D020932:Nerve Growth Factor; D019098:Telomerase