title: Effect of monomeric adiponectin on cardiac function and perfusion in anesthetized pig.
abstract: Adiponectin, the most abundant adipokine released by adipose tissue, appears to play an important role in the regulation of vascular endothelial and cardiac function. To date, however, the physiological effects of human monomeric adiponectin on the coronary vasculature and myocardial systo-diastolic function, as well as on parasympathetic/sympathetic involvement and nitric oxide (NO) release, have not yet been investigated. Thus, we planned to determine the primary in vivo effects of human monomeric adiponectin on coronary blood flow and cardiac contractility/relaxation and the related role of autonomic nervous system, adiponectin receptors, and NO. In 30 anesthetized pigs, human monomeric adiponectin was infused into the left anterior descending coronary artery at constant heart rate and arterial blood pressure, and the effects on coronary blood flow, left ventricular systo-diastolic function, myocardial oxygen metabolism, and NO release were examined. The mechanisms of the observed hemodynamic responses were also analyzed by repeating the highest dose of human monomeric adiponectin infusion after autonomic nervous system and NO blockade, and after specific adiponectin 1 receptor antagonist administration. Intracoronary human monomeric adiponectin caused dose-related increases of coronary blood flow and cardiac function. Those effects were accompanied by increased coronary NO release and coronary adiponectin levels. Moreover, the vascular effects of the peptide were prevented by blockade of β2-adrenoceptors and NO synthase, whereas all effects of human monomeric adiponectin were prevented by adiponectin 1 receptor inhibitor. In conclusion, human monomeric adiponectin primarily increased coronary blood flow and cardiac systo-diastolic function through the involvement of specific receptors, β2-adrenoceptors, and NO release.
authors.affiliation: Laboratory of Physiology and Experimental SurgeryDepartment of Translational Medicine, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carita, Corso Mazzini 36, I-28100 Novara, ItalyPediatric UnitDepartment of Health Sciences, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 36, I-28100 Novara, Italy grossini@med.unipmn.it.
authors.forename: Elena
authors.initials: E
authors.lastname: Grossini
authors.affiliation: Laboratory of Physiology and Experimental SurgeryDepartment of Translational Medicine, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carita, Corso Mazzini 36, I-28100 Novara, ItalyPediatric UnitDepartment of Health Sciences, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 36, I-28100 Novara, Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Prodam
authors.affiliation: Laboratory of Physiology and Experimental SurgeryDepartment of Translational Medicine, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carita, Corso Mazzini 36, I-28100 Novara, ItalyPediatric UnitDepartment of Health Sciences, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 36, I-28100 Novara, Italy.
authors.forename: Gillian Elisabeth
authors.initials: GE
authors.lastname: Walker
authors.affiliation: Laboratory of Physiology and Experimental SurgeryDepartment of Translational Medicine, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carita, Corso Mazzini 36, I-28100 Novara, ItalyPediatric UnitDepartment of Health Sciences, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 36, I-28100 Novara, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Sigaudo
authors.affiliation: Laboratory of Physiology and Experimental SurgeryDepartment of Translational Medicine, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carita, Corso Mazzini 36, I-28100 Novara, ItalyPediatric UnitDepartment of Health Sciences, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 36, I-28100 Novara, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Farruggio
authors.affiliation: Laboratory of Physiology and Experimental SurgeryDepartment of Translational Medicine, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carita, Corso Mazzini 36, I-28100 Novara, ItalyPediatric UnitDepartment of Health Sciences, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 36, I-28100 Novara, Italy.
authors.forename: Kevin
authors.initials: K
authors.lastname: Bellofatto
authors.affiliation: Laboratory of Physiology and Experimental SurgeryDepartment of Translational Medicine, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carita, Corso Mazzini 36, I-28100 Novara, ItalyPediatric UnitDepartment of Health Sciences, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 36, I-28100 Novara, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Marotta
authors.affiliation: Laboratory of Physiology and Experimental SurgeryDepartment of Translational Medicine, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carita, Corso Mazzini 36, I-28100 Novara, ItalyPediatric UnitDepartment of Health Sciences, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 36, I-28100 Novara, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Molinari
authors.affiliation: Laboratory of Physiology and Experimental SurgeryDepartment of Translational Medicine, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carita, Corso Mazzini 36, I-28100 Novara, ItalyPediatric UnitDepartment of Health Sciences, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 36, I-28100 Novara, Italy.
authors.forename: David
authors.initials: D
authors.lastname: Mary
authors.affiliation: Laboratory of Physiology and Experimental SurgeryDepartment of Translational Medicine, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carita, Corso Mazzini 36, I-28100 Novara, ItalyPediatric UnitDepartment of Health Sciences, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 36, I-28100 Novara, Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Bona
authors.affiliation: Laboratory of Physiology and Experimental SurgeryDepartment of Translational Medicine, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carita, Corso Mazzini 36, I-28100 Novara, ItalyPediatric UnitDepartment of Health Sciences, University Eastern Piedmont 'A. Avogadro', Via Solaroli 17, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 36, I-28100 Novara, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Vacca
mesh_terms: D052242:Adiponectin / Q000494:pharmacology*; D000777:Anesthetics; D000818:Animals; D001341:Autonomic Nervous System / Q000187:drug effects / Q000502:physiology; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D003331:Coronary Vessels / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D006321:Heart / Q000187:drug effects* / Q000502:physiology*; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D006801:Humans; D023421:Models, Animal; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D054419:Receptors, Adiponectin / Q000187:drug effects / Q000502:physiology; D012039:Regional Blood Flow / Q000187:drug effects* / Q000502:physiology*; D013552:Swine; D014474:Unconsciousness
chemical_list: D052242:Adiponectin; D000777:Anesthetics; D054419:Receptors, Adiponectin; D009569:Nitric Oxide
keywords: adipokine; autonomic nervous system; heart function; nitric oxide