title: Effect of hexavalent chromium on histone biotinylation in human bronchial epithelial cells.
abstract: Chromium is a potent human mutagen and carcinogen. The capability of chromium to cause cancers has been known for more than a century, and numerous epidemiological studies have been performed to determine its carcinogenicity. In the post-genome era, cancer has been found to relate to epigenetic mutations. However, very few researches have focused on hexavalent chromium (Cr(VI))-induced epigenetic alterations. The present study was designed to investigate whether Cr(VI) would affect the level of a newfound epigenetic modification: histone biotinylation. Histone acetylation and histone biotinylation were studied in detail using human bronchial epithelial (16HBE) cells as an in vitro model after Cr(VI) treatment. Our study showed that Cr(VI) treatment decreased histone acetylation level in 16HBE cells. In addition, low doses of Cr(VI) (≤0.6μM) elevated the level of histone biotinylation. Furthermore, immunoblot analysis of biotinidase (BTD), a major protein which maintains homeostasis of histone biotinylation, showed that the distribution of BTD became less even and more concentrated at the nuclear periphery in cells exposed to Cr(VI). Moreover, Cr(VI)-induced histone deacetylation may take part in the regulation of histone biotinylation. Together, our study provides new insight into the mechanisms of Cr(VI)-induced epigenetic regulation that may contribute to the chemoprevention of Cr(VI)-induced cancers and may have important implications for epigenetic therapy.
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China; Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Health Toxicology, Laboratory of Modern Toxicology, Shenzhen Centre for Disease Control and Prevention, Shenzhen 518055, China; College of Food Science and Technology, Hunan Agricultural University, East Renmin Road, Changsha 410128, Hunan, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xia
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China; Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Health Toxicology, Laboratory of Modern Toxicology, Shenzhen Centre for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Xiao-Hu
authors.initials: XH
authors.lastname: Ren
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Health Toxicology, Laboratory of Modern Toxicology, Shenzhen Centre for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Zhi-Xiong
authors.initials: ZX
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Health Toxicology, Laboratory of Modern Toxicology, Shenzhen Centre for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Lin-Qing
authors.initials: LQ
authors.lastname: Yang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Health Toxicology, Laboratory of Modern Toxicology, Shenzhen Centre for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Huang
authors.affiliation: College of Food Science and Technology, Hunan Agricultural University, East Renmin Road, Changsha 410128, Hunan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Pang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Health Toxicology, Laboratory of Modern Toxicology, Shenzhen Centre for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: De-Sheng
authors.initials: DS
authors.lastname: Wu
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China.
authors.forename: You-Li
authors.initials: YL
authors.lastname: Tan
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Health Toxicology, Laboratory of Modern Toxicology, Shenzhen Centre for Disease Control and Prevention, Shenzhen 518055, China. Electronic address: junii8@126.com.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Liu
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China. Electronic address: public_xb@sina.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
mesh_terms: D000107:Acetylation; D043523:Biotinidase / Q000378:metabolism; D019921:Biotinylation; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D002840:Chromates / Q000633:toxicity*; D002857:Chromium / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D044127:Epigenesis, Genetic / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006801:Humans; D017680:Potassium Compounds / Q000633:toxicity*; D013997:Time Factors
chemical_list: D002840:Chromates; D006657:Histones; D017680:Potassium Compounds; D002857:Chromium; C074702:chromium hexavalent ion; C027373:potassium chromate(VI); D043523:Biotinidase
keywords: Chromium(VI); Histone acetylation; Histone biotinylation