title: Tamlana sedimentorum sp. nov., isolated from shallow sand sediments of the Sea of Japan.
abstract: An aerobic, Gram-stain-negative, saffron-pigmented, non-motile bacterial strain KMM 9545(T), was isolated from a marine sand sediment sample obtained from the Sea of Japan seashore and characterized in a taxonomic study using a polyphasic approach. The 16S rRNA gene sequence of strain KMM 9545(T) showed a high level of similarity to species of the genus Gaetbulibacter (95.1-96.2%), the type strains of species of the genus Tamlana (94.9-96.1%) and members of the genus Algibacter (94.8-96.1%). Phylogenetic analysis based on the 16S rRNA gene sequences positioned strain KMM 9545(T) as a distinct lineage in the cluster comprising species of the genus Tamlana. Strain KMM 9545(T) grew at temperatures between 5-36 °C and in the presence of 2-4% (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C(15 : 0), iso-C(15 : 1), iso-C(17 : 1) 3-OH, iso-C(15 : 0) 3-OH and iso-C(15 : 0) 2-OH as the major fatty acids. The genomic DNA G+C content was 31.3 mol%. On the basis of the phenotypic characteristics and phylogenetic distance, it can be concluded that strain KMM 9545(T) represents a novel species of the genus Tamlana, for which the name Tamlana sedimentorum sp. nov. is proposed. The type strain is KMM 9545(T) ( = NRIC 0921(T) = JCM 19808(T)).
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok 690022, Russia.
authors.forename: Lyudmila A
authors.initials: LA
authors.lastname: Romanenko
authors.affiliation: NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Tanaka
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok 690022, Russia.
authors.forename: Valeriya V
authors.initials: VV
authors.lastname: Kurilenko
authors.affiliation: Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690041, Russia.
authors.forename: Vassilii I
authors.initials: VI
authors.lastname: Svetashev
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification*; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6; D012822:Silicon Dioxide