title: Expression profile of hypothalamic neuropeptides in chicken lines selected for high or low residual feed intake.
abstract: The R(+) and R(-) chicken lines have been divergently selected for high (R(+)) or low (R(-)) residual feed intake. For the same body weight and egg production, the R(+) chickens consume 40% more food than their counterparts R(-) lines. In the present study we sought to determine the hypothalamic expression profile of feeding-related neuropeptides in these lines maintained under fed or food-deprived conditions. In the fed condition, the suppressor of cytokine signaling 3 (SOCS3) was 17-fold lower (P<0.05) and the ghrelin receptor was 7-fold higher (P<0.05) in R(+) compared to R(-) chicken lines. The hypothalamic expression of the other studied genes remained unchanged between the two lines. In the fasted state, orexigenic neuropeptide Y and agouti-related peptide were more responsive, with higher significant levels in the R(+) compared to R(-) chickens, while no significant differences were seen for the anorexigenic neuropeptides pro-opiomelanocortin and corticotropin releasing hormone. Interestingly, C-reactive protein, adiponectin receptor 1 and ghrelin receptor gene expression were significantly higher (12-, 2- and 3-folds, respectively), however ghrelin and melanocortin 5 receptor mRNA levels were lower (4- and 2-folds, P=0.05 and P=0.03, respectively) in R(+) compared to R(-) animals. We identified several key feeding-related genes that are differently expressed in the hypothalamus of R(+) and R(-) chickens and that might explain the difference in feed intake observed between the two lines.
authors.affiliation: Division of Livestock-Nutrition-Quality, Department of Biosystems, KU Leuven, Kasteelpark Arenberg, 30, 3001 Leuven, Belgium. Electronic address: petrasintubin@yahoo.com.
authors.forename: P
authors.initials: P
authors.lastname: Sintubin
authors.affiliation: Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. Electronic address: esgreene@uark.edu.
authors.forename: E
authors.initials: E
authors.lastname: Greene
authors.affiliation: INRA, UR83 Recherches Avicoles, F-37380 Nouzilly, France. Electronic address: anne.collin@tours.inra.fr.
authors.forename: A
authors.initials: A
authors.lastname: Collin
authors.affiliation: INRA/AgroParisTech, UMR 1313 GABI, Division of Animal Genetics, 78352 Jouy-en-Josas, France. Electronic address: andre.bordas@yahoo.fr.
authors.forename: A
authors.initials: A
authors.lastname: Bordas
authors.affiliation: INRA/AgroParisTech, UMR 1313 GABI, Division of Animal Genetics, 78352 Jouy-en-Josas, France. Electronic address: Tatiana.zerjal@jouy.inra.fr.
authors.forename: T
authors.initials: T
authors.lastname: Zerjal
authors.affiliation: INRA, UR83 Recherches Avicoles, F-37380 Nouzilly, France. Electronic address: sophie.tesseraud@tours.inra.fr.
authors.forename: S
authors.initials: S
authors.lastname: Tesseraud
authors.affiliation: Division of Livestock-Nutrition-Quality, Department of Biosystems, KU Leuven, Kasteelpark Arenberg, 30, 3001 Leuven, Belgium. Electronic address: johan.buyse@biw.kuleuven.be.
authors.forename: J
authors.initials: J
authors.lastname: Buyse
authors.affiliation: Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. Electronic address: dridi@uark.edu.
authors.forename: S
authors.initials: S
authors.lastname: Dridi
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology*; D004435:Eating / Q000235:genetics* / Q000502:physiology; D005260:Female; D005838:Genotype; D006963:Hyperphagia / Q000235:genetics / Q000523:psychology; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D009479:Neuropeptides / Q000096:biosynthesis* / Q000235:genetics; D009752:Nutritional Status
chemical_list: D009479:Neuropeptides
keywords: Chickens; Feed intake; Food deprivation; Hyperphagia; Hypothalamus; Neuropeptides