title: Cell sheet approach for tissue engineering and regenerative medicine.
abstract: After the biotech medicine era, regenerative medicine is expected to be an advanced medicine that is capable of curing patients with difficult-to-treat diseases and physically impaired function. Our original scaffold-free cell sheet-based tissue engineering technology enables transplanted cells to be engrafted for a long time, while fully maintaining their viability. This technology has already been applied to various diseases in the clinical setting, including the cornea, esophagus, heart, periodontal ligament, and cartilage using autologous cells. Transplanted cell sheets not only replace the injured tissue and compensate for impaired function, but also deliver growth factors and cytokines in a spatiotemporal manner over a prolonged period, which leads to promotion of tissue repair. Moreover, the integration of stem cell biology and cell sheet technology with sufficient vascularization opens possibilities for fabrication of human three-dimensional vascularized dense and intact tissue grafts for regenerative medicine to parenchymal organs.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo 162-8666, Japan; Department of Cardiology, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo 162-8666, Japan. Electronic address: matsuura.katsuhisa@twmu.ac.jp.
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Matsuura
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo 162-8666, Japan. Electronic address: utoh-yamada.rie@twmu.ac.jp.
authors.forename: Rie
authors.initials: R
authors.lastname: Utoh
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo 162-8666, Japan. Electronic address: nagase.kenichi@twmu.ac.jp.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Nagase
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo 162-8666, Japan. Electronic address: tokano@twmu.ac.jp.
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D060846:Cell Engineering / Q000379:methods*; D003922:Diabetes Mellitus, Type 1 / Q000628:therapy*; D006321:Heart / Q000502:physiology; D006331:Heart Diseases / Q000628:therapy*; D006801:Humans; D016381:Islets of Langerhans Transplantation; D008099:Liver / Q000166:cytology / Q000502:physiology; D008107:Liver Diseases / Q000628:therapy*; D032446:Myoblasts / Q000637:transplantation; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods; D033581:Stem Cell Transplantation; D054457:Tissue Scaffolds
keywords: Cell sheet; Regenerative medicine