title: Fluorogenic probes for live-cell imaging of the cytoskeleton.
abstract: We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.
authors.affiliation: 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2].
authors.forename: Gražvydas
authors.identifier: 0000000271761793
authors.initials: G
authors.lastname: Lukinavičius
authors.affiliation: 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland. [3].
authors.forename: Luc
authors.initials: L
authors.lastname: Reymond
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Elisa
authors.initials: E
authors.lastname: D'Este
authors.affiliation: Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Anastasiya
authors.initials: A
authors.lastname: Masharina
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Göttfert
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Haisen
authors.initials: H
authors.lastname: Ta
authors.affiliation: Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University, Jena, Germany.
authors.forename: Angelika
authors.initials: A
authors.lastname: Güther
authors.affiliation: Bioimaging and Optics Platform, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Mathias
authors.initials: M
authors.lastname: Fournier
authors.affiliation: Max Planck Institute of Molecular Physiology, Dortmund, Germany.
authors.forename: Stefano
authors.initials: S
authors.lastname: Rizzo
authors.affiliation: Max Planck Institute of Molecular Physiology, Dortmund, Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Waldmann
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.
authors.forename: Claudia
authors.initials: C
authors.lastname: Blaukopf
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Sommer
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Gerlich
authors.affiliation: Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University, Jena, Germany.
authors.forename: Hans-Dieter
authors.initials: HD
authors.lastname: Arndt
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
authors.affiliation: 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland.
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
mesh_terms: D000199:Actins / Q000737:chemistry*; D000818:Animals; D001369:Axons / Q000737:chemistry; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure; D005260:Female; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D009474:Neurons / Q000166:cytology; D051381:Rats; D012235:Rhodamines / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D014404:Tubulin / Q000737:chemistry*
chemical_list: D000199:Actins; D005456:Fluorescent Dyes; D012235:Rhodamines; D014404:Tubulin; D012825:Silicon