title: Characterisation of bacterioplankton communities in the meltwater ponds of Bratina Island, Victoria Land, Antarctica.
abstract: A unique collection of Antarctic aquatic environments (meltwater ponds) lies in close proximity on the rock and sediment-covered undulating surface of the McMurdo Ice Shelf, near Bratina Island (Victoria Land, Antarctica). During the 2009-10 mid-austral summer, sets of discrete water samples were collected across the vertical geochemical gradients of five meltwater ponds (Egg, P70E, Legin, Salt and Orange) for geochemical and microbial community structure analysis. Bacterial DNA fingerprints (using Automated Ribosomal Intergenic Spacer Analysis) statistically clustered communities within ponds based on anosim (R = 0.766, P = 0.001); however, one highly stratified pond (Egg) had two distinct depth-related bacterial communities (R = 0.975, P = 0.008). 454 pyrosequencing at three depths within Egg also identified phylum level shifts and increased diversity with depth, Bacteroidetes being the dominant phyla in the surface sample and Proteobacteria being dominant in the bottom two depths. best analysis, which attempts to link community structure and the geochemistry of a pond, identified conductivity and pH individually, and to a lesser extent Ag(109) , NO2 and V(51) as dominant influences to the microbial community structure in these ponds. Increasing abundances of major halo-tolerant OTUs across the strong conductivity gradient reinforce it as the primary driver of community structure in this study.
authors.affiliation: International Centre for Terrestrial Antarctic Research, School of Science, University of Waikato, Hamilton, New Zealand.
authors.forename: Stephen D J
authors.initials: SD
authors.lastname: Archer
authors.forename: Ian R
authors.initials: IR
authors.lastname: McDonald
authors.forename: Craig W
authors.initials: CW
authors.lastname: Herbold
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Cary
mesh_terms: D000864:Antarctic Regions; D041963:Bacteroidetes / Q000235:genetics*; D004553:Electric Conductivity; D059014:High-Throughput Nucleotide Sequencing; D006863:Hydrogen-Ion Concentration; D058889:Molecular Typing; D010802:Phylogeny; D061005:Ponds / Q000737:chemistry / Q000382:microbiology*; D020560:Proteobacteria / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Antarctic; community; meltwater; microorganism; planktonic