title: A fluorescent probe for imaging symmetric and asymmetric cell division in neurosphere formation.
abstract: We report here a novel fluorescent chemical probe which stains distinct neural stem/progenitor cells (NSPCs) by binding to acid ceramidase in mouse neurospheres. is distributed evenly or unevenly to the daughter cells during multiple mitoses enabling the live imaging of symmetric and asymmetric divisions of isolated NSPCs.
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore.
authors.forename: Seong-Wook
authors.initials: SW
authors.lastname: Yun
authors.forename: Cheryl
authors.initials: C
authors.lastname: Leong
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Bi
authors.forename: Hyung-Ho
authors.initials: HH
authors.lastname: Ha
authors.forename: Yuan Hong
authors.initials: YH
authors.lastname: Yu
authors.forename: Yee Ling
authors.initials: YL
authors.lastname: Tan
authors.forename: Gunaseelan
authors.initials: G
authors.lastname: Narayanan
authors.forename: Shvetha
authors.initials: S
authors.lastname: Sankaran
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Srivats
authors.initials: S
authors.lastname: Hariharan
authors.forename: Sohail
authors.initials: S
authors.lastname: Ahmed
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D000818:Animals; D060049:Asymmetric Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000737:chemistry*; D051379:Mice; D058953:Neural Stem Cells / Q000737:chemistry* / Q000502:physiology; D059008:Time-Lapse Imaging / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes