title: [Roles of ceruloplasmin in silica-induced JNK/ERK/AP-1 cell signaling pathway change].
abstract: OBJECTIVE
  To investigate the roles of ceruloplasmin (Cp) in JNK/ERK/ AP-1 cell signaling pathway change in human embryonic lung fibroblasts (HELFs) induced by silica.
  
  
  METHODS
  Cp stimulated HELFs in different time points (before 1 h, accompanied with or after 1 h of silica-adding). HELFs were divided into these groups: control group, silica(100 microg/ml for 24 h) group, Cp (30 microg/ml for 24 h) group and silica plus Cp (100 microg/ml silica plus 30 microg/ml Cp) group. DN-JNK cells and DN-ERK cells (cells were transfected with dominant negative mutant plasmid) contained these groups: control group, silica group, silica plus Cp group. MTT assay was used to detect the effects of Cp on silica-induced cell proliferation. Western blot assay was performed to detect the levels of JNK, ERK, c-Jun, c-Fos and their phosphorylated levels.
  
  
  RESULTS
  Cp promoted cell proliferation induced by silica when silica stimulated HELFs 1 h then adding to Cp. Cp significantly increased silica-induced the high levels of JNK, ERK and phosphorylated JNK (p-JNK), p-ERK, p-c-Jun and p-c-fos protein. After inhibition of JNK or ERK, silica-and-Cp-induced cell proliferation was markedly decreased. When suppressing JNK protein, the increased levels of p-JNK, p-c-Jun and p-c-fos protein was not observed. The high levels of p-ERK, p-c-Jun and p-c-fos protein were decreased when inhibiting ERK protein.
  
  
  CONCLUSIONS
  Cp could further strengthen silica-induced cell proliferation by JNK/c-Jun/c-Fos and ERK/c-Jun cell signaling pathway.
authors.forename: Meng
authors.initials: M
authors.lastname: Ye
authors.forename: Xianan
authors.initials: X
authors.lastname: Zhang
authors.forename: Yuegang
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Jia
authors.forename: Bingci
authors.initials: B
authors.lastname: Liu
mesh_terms: D002450:Cell Communication; D049109:Cell Proliferation; D002478:Cells, Cultured; D002570:Ceruloplasmin / Q000502:physiology*; D005347:Fibroblasts; D006801:Humans; D008168:Lung; D020935:MAP Kinase Signaling System; D010084:Oxidation-Reduction; D010766:Phosphorylation; D016760:Proto-Oncogene Proteins c-fos; D015398:Signal Transduction; D012822:Silicon Dioxide / Q000633:toxicity*; D018808:Transcription Factor AP-1
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D018808:Transcription Factor AP-1; D012822:Silicon Dioxide; D002570:Ceruloplasmin