title: Phylogenetic relationships between pinworms (Nematoda: Enterobiinae) parasitising the critically endangered orang-utan, according to the characterisation of molecular genomic and mitochondrial markers.
abstract: Pinworms (Nematoda: Enterobiinae) include 52 species parasitising primates throughout the world. In the present study, we performed the first ever molecular analysis to investigate the phylogenetic position of recently described pinworms parasitising the Sumatran orang-utan. The phylogenetic analysis of mitochondrial CO1 and chromosomal 18S rDNA and ITS1 regions could support the independent status of several Nematoda species. Our molecular data clearly suggest that Enterobius (Colobenterobius) buckleyi and Lemuricola (Protenterobius) pongoi together with Pongobius hugoti form separate clades among other studied species, which significantly supports the hypothesis of recently described new species parasitising the orang-utan (Pongo abelii and Pongo pygmaeus). The phylogenetic tree based on cytochrome oxidase 1 (CO1) gene variability showed possible close relationships between L. (Protenterobius) pongoi and P. hugoti; thus, we can assume that these species could have initially diverged in sympatry from a common ancestor.
authors.affiliation: Department of Botany and Zoology, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic, foitova@sci.muni.cz.
authors.forename: Ivona
authors.initials: I
authors.lastname: Foitová
authors.forename: Kristína
authors.initials: K
authors.lastname: Civáňová
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Baruš
authors.forename: Wisnu
authors.initials: W
authors.lastname: Nurcahyo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D021901:DNA, Intergenic / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D017229:Enterobiasis / Q000469:parasitology / Q000662:veterinary*; D004757:Enterobius / Q000145:classification* / Q000235:genetics; D005819:Genetic Markers; D049810:Genetic Speciation; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D057080:Pongo abelii / Q000469:parasitology*; D011148:Pongo pygmaeus / Q000469:parasitology*
chemical_list: D021901:DNA, Intergenic; D005819:Genetic Markers; D003576:Electron Transport Complex IV