title: Fluorescent boronic acid terminated polymer grafted silica particles synthesized via click chemistry for affinity separation of saccharides.
abstract: Boronic acids are important for effective separation of biological active cis-diols. For the purpose of constructing a new type of saccharide-sensitive material which can not only provide convenient separation but also improve the access of boronic acid to guest molecules, the fluorogenic boronic acid terminated, thermo-sensitive polymers (BA-polyNIPAm) were grafted to an alkyne modified silica gel through the exploitation of click chemistry. The BA-polyNIPAm grafted silica gel (BA-polyNIPAm-SG) was characterized by FT-IR, fluorescence spectra, fluorescence microscopy, elemental analysis (EA), thermal gravimetric analysis (TGA), scanning electron microscope (SEM) and so on. BA-polyNIPAm-SG displayed affinity binding ability for saccharides under physiological pH value and allowed saccharides to be conveniently separated from solution. The maximum binding capacities for fructose and glucose are 83.2 μmol/g and 70.4 μmol/g polymer, respectively. The intensity of fluorescence emission of BA-polyNIPAm-SG increased with the increasing of fructose concentration. The present study provides a new kind of composite material which contains moveable and flexible grippers for recognizing and binding guest molecules.
authors.affiliation: Department of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China; Key Laboratory of Functional Organometallic Materials of Hunan Province University, Hengyang 421008, PR China. Electronic address: 897061147@qq.com.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China; Key Laboratory of Functional Organometallic Materials of Hunan Province University, Hengyang 421008, PR China.
authors.forename: Peihong
authors.initials: P
authors.lastname: Deng
authors.affiliation: Department of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China; Key Laboratory of Functional Organometallic Materials of Hunan Province University, Hengyang 421008, PR China.
authors.forename: Siping
authors.initials: S
authors.lastname: Tang
authors.affiliation: Department of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China; Key Laboratory of Functional Organometallic Materials of Hunan Province University, Hengyang 421008, PR China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Li
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry / Q000302:isolation & purification*; D057930:Click Chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007536:Isomerism; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry
chemical_list: D001897:Boronic Acids; D002241:Carbohydrates; D005456:Fluorescent Dyes; D011108:Polymers; D012996:Solutions; D012822:Silicon Dioxide
keywords: Affinity binding; Boronic acid; Click chemistry; Fluorescence; Saccharide