title: Genetic variations of MMP9 gene and intracerebral hemorrhage outcome: a cohort study in Chinese Han population.
abstract: OBJECTIVE
  To investigate the association between genetic variations of matrix metalloproteinase 9 (MMP9) gene and intracerebral hemorrhage (ICH) outcome in Chinese Han population.
  
  
  METHODS
  The clinical data and peripheral blood samples from the patients with ICH were collected. The patients were followed up for 3 months, and poor outcome was defined as death or dependency (modified Rankin scale score of 3-6). MassARRAY Analyser was used to genotype the tagger single nucleotide polymorphisms (SNPs) of MMP9 gene. Construction of haplotypes and genetic comparisons were performed by employing PLINK 1.0.7 software.
  
  
  RESULTS
  181 patients with ICH were recruited between September 2009 and October 2010. Information on genetic variations and follow-up assessments were available for 169 (93.4%) patients. Independent patients at 90 days were younger than those who died or dependent (57.82 ± 13.47 vs. 66.99 ± 11.49 years, p<0.01). In addition, independent patients had lower National Institutes of Health Stroke Scale (NIHSS) score (4[1-6] vs. 13[9-19], p<0.01). There were no significant associations (all p>0.05) between MMP9 genetic variations (alleles, genotypes and haplotypes) and ICH outcome after adjustment for conventional risk factors.
  
  
  CONCLUSIONS
  The genetic variations of MMP9 gene were not significantly associated with ICH outcome at 90 days in Chinese Han population.
authors.affiliation: Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China; Department of Neurology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Neurology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
authors.forename: Junshan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.affiliation: The George Institute for Global Health, Royal Prince Alfred Hospital, University of Sydney, Sydney, NSW 2050, Australia.
authors.forename: Hisatomi
authors.initials: H
authors.lastname: Arima
authors.affiliation: Department of Neurology, The Third People's Hospital of Mianyang, Luzhou Medical College, Mianyang 621000, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China. Electronic address: wyplmh@hotmail.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000208:ethnology / Q000235:genetics; D002543:Cerebral Hemorrhage / Q000235:genetics*; D016009:Chi-Square Distribution; D015331:Cohort Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D020780:Matrix Metalloproteinase 9
keywords: Genetic variation; Haplotype; Intracerebral hemorrhage; Matrix metalloproteinase 9; Outcome; Single nucleotide polymorphism