title: A method for generating highly multiplexed ChIP-seq libraries.
abstract: BACKGROUND
  The barcoding of next generation sequencing libraries has become an essential part of the experimental design. Barcoding not only allows the sequencing of more than one sample per lane, but also reduces technical bias. However, current barcoding strategies impose significant limitations and/or technical barriers in their implementation for ChIP-sequencing.
  
  
  RESULTS
  Converting Y-shaped sequencing adapters to double stranded DNA prior to agarose gel size selection reduces adapter dimer contamination and quantitating the number of cycles required for amplification of the library with qPCR prior to library amplification eliminates library over-amplification.
  
  
  CONCLUSIONS
  We describe an efficient and cost effective method for making barcoded ChIP-seq libraries for sequencing on the Illumina platform.
authors.forename: Ethan
authors.initials: E
authors.lastname: Ford
authors.forename: Chrysa
authors.initials: C
authors.lastname: Nikopoulou
authors.forename: Antonis
authors.initials: A
authors.lastname: Kokkalis
authors.affiliation: Biomedical Research Foundation, Academy of Athens, 4 Soranou Efesiou Street, Athens 11527, Greece. thanos@bioacademy.gr.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Thanos
mesh_terms: D047369:Chromatin Immunoprecipitation