title: Morphogenesis and production of enzymes by Penicillium echinulatum in response to different carbon sources.
abstract: The effect of different carbon sources on morphology and cellulase and xylanase production of Penicillium echinulatum was evaluated in this work. Among the six carbon sources studied, cellulose and sugar cane bagasse were the most suitable for the production of filter paper activity, endoglucanases, xylanases, and β-glucosidases. However, sucrose and glucose showed β -glucosidase activities similar to those obtained with the insoluble sources. The polyacrylamide gels proved the enzymatic activity, since different standards bands were detected in the media mentioned above. Regarding morphology, it was observed that the mycelium in a dispersed form provided the greatest enzymatic activity, possibly due to greater interaction between the substrate and hyphae. These data are important in understanding the physiology of fungi and could contribute to obtaining enzyme with potential application in the technology of second generation ethanol.
authors.affiliation: Enzyme and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, 1130, Francisco Getúlio Vargas Street, 95070-560 Caxias do Sul, RS, Brazil.
authors.forename: Willian Daniel Hahn
authors.initials: WD
authors.lastname: Schneider
authors.affiliation: Enzyme and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, 1130, Francisco Getúlio Vargas Street, 95070-560 Caxias do Sul, RS, Brazil.
authors.forename: Laísa
authors.initials: L
authors.lastname: dos Reis
authors.affiliation: Enzyme and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, 1130, Francisco Getúlio Vargas Street, 95070-560 Caxias do Sul, RS, Brazil.
authors.forename: Marli
authors.identifier: 0000-0001-7410-4337
authors.initials: M
authors.lastname: Camassola
authors.affiliation: Enzyme and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, 1130, Francisco Getúlio Vargas Street, 95070-560 Caxias do Sul, RS, Brazil.
authors.forename: Aldo José Pinheiro
authors.initials: AJ
authors.lastname: Dillon
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000096:biosynthesis*; D010407:Penicillium / Q000166:cytology* / Q000201:enzymology*; D031786:Saccharum / Q000737:chemistry
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D002244:Carbon; D002482:Cellulose; C027433:bagasse; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases