title: Hepatectomised patient sera promote hepatocyte differentiation of human-induced pluripotent stem cells.
abstract: BACKGROUND
  Human induced pluripotent stem cells, which can be differentiated into hepatocyte-like cells, could provide a source for liver regeneration and bio-artificial liver devices. However, the functionality of hepatocyte-like cells is significantly lower than that of primary hepatocytes.
  
  
  OBJECTIVE
  To investigate whether serum from patients undergoing hepatectomy might promote differentiation from human induced pluripotent stem cells to hepatocyte-like cells.
  
  
  METHODS
  Serum from patients undergoing hepatectomy (acquired pre-hepatectomy and 3 hours, 1 day and 3 days post-hepatectomy) was used to replace foetal bovine serum when differentiating human induced pluripotent stem cells into hepatocyte-like cells. Properties of hepatocyte-like cells were assessed and compared with cells cultured in foetal bovine serum.
  
  
  RESULTS
  The differentiation efficiency and functionality of hepatocyte-like cells cultured in human serum 3 hours and 1 day post-hepatectomy were superior to those cultured in foetal bovine serum and human serum pre-hepatectomy. Human serum 3 days post-hepatectomy had an equal effect to that of human serum pre-hepatectomy. Some cytochrome P450 isozyme transcript levels of hepatocyte-like cells cultured in human serum were higher than those cultured in foetal bovine serum.
  
  
  CONCLUSIONS
  Human serum, particularly that acquired relatively soon after hepatectomy, can enhance the differentiation efficiency and functionality of hepatocyte-like cells derived from human induced pluripotent stem cells.
authors.affiliation: The Third Central Clinical College of Tianjin Medical University, Tianjin, China; The Key Laboratory of Artificial Cells, Institute of Hepatobiliary Disease, The Third Central Hospital of Tianjin, Tianjin, China; Department of Hepatobiliary Surgery, The Third Central Hospital of Tianjin, Tianjin, China.
authors.forename: Qianzhe
authors.initials: Q
authors.lastname: Xing
authors.affiliation: The Key Laboratory of Artificial Cells, Institute of Hepatobiliary Disease, The Third Central Hospital of Tianjin, Tianjin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.affiliation: The Key Laboratory of Artificial Cells, Institute of Hepatobiliary Disease, The Third Central Hospital of Tianjin, Tianjin, China.
authors.forename: Yingtang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Cardiology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Sui
authors.initials: S
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Artificial Cells, Institute of Hepatobiliary Disease, The Third Central Hospital of Tianjin, Tianjin, China.
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Hepatobiliary Surgery, The Third Central Hospital of Tianjin, Tianjin, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, The Third Central Hospital of Tianjin, Tianjin, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Department of Hepatobiliary Surgery, The Third Central Hospital of Tianjin, Tianjin, China.
authors.forename: Guiming
authors.initials: G
authors.lastname: Shu
authors.affiliation: Department of Hepatobiliary Surgery, The Third Central Hospital of Tianjin, Tianjin, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lou
authors.affiliation: Department of Hepatobiliary Surgery, The Third Central Hospital of Tianjin, Tianjin, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Artificial Cells, Institute of Hepatobiliary Disease, The Third Central Hospital of Tianjin, Tianjin, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: The Third Central Clinical College of Tianjin Medical University, Tianjin, China; The Key Laboratory of Artificial Cells, Institute of Hepatobiliary Disease, The Third Central Hospital of Tianjin, Tianjin, China; Department of Hepatobiliary Surgery, The Third Central Hospital of Tianjin, Tianjin, China. Electronic address: zhi-du@163.com.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Du
mesh_terms: D000418:Albumins / Q000096:biosynthesis; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D015870:Gene Expression; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D044967:Serum; D014508:Urea / Q000378:metabolism
chemical_list: D000418:Albumins; D012333:RNA, Messenger; D014508:Urea; D003577:Cytochrome P-450 Enzyme System
keywords: Differentiation; Hepatectomy; Hepatocyte; Human induced pluripotent stem cells