title: Vigna unguiculata is nodulated in Spain by endosymbionts of Genisteae legumes and by a new symbiovar (vignae) of the genus Bradyrhizobium.
abstract: Vigna unguiculata was introduced into Europe from its distribution centre in Africa, and it is currently being cultivated in Mediterranean regions with adequate edapho-climatic conditions where the slow growing rhizobia nodulating this legume have not yet been studied. Previous studies based on rrs gene and ITS region analyses have shown that Bradyrhizobium yuanmingense and B. elkanii nodulated V. unguiculata in Africa, but these two species were not found in this study. Using the same phylogenetic markers it was shown that V. unguiculata, a legume from the tribe Phaseolae, was nodulated in Spain by two species of group I, B. cytisi and B. canariense, which are common endosymbionts of Genisteae in both Europe and Africa. These species have not been found to date in V. unguiculata nodules in its African distribution centres. All strains from Bradyrhizobium group I isolated in Spain belonged to the symbiovar genistearum, which is found at present only in Genisteae legumes in both Africa and Europe. V. unguiculata was also nodulated in Spain by a strain from Bradyrhizobium group II that belonged to a novel symbiovar (vignae). Some African V. unguiculata-nodulating strains also belonged to this proposed new symbiovar.
authors.affiliation: Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Bejarano
authors.affiliation: Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain; Unidad Asociada Universidad de Salamanca-CSIC 'Interacción Planta-Microorganismo', Salamanca, Spain.
authors.forename: Martha-Helena
authors.initials: MH
authors.lastname: Ramírez-Bahena
authors.affiliation: Unidad Asociada Universidad de Salamanca-CSIC 'Interacción Planta-Microorganismo', Salamanca, Spain; Departamento de Microbiología y Genética, Lab. 209, Universidad de Salamanca, Edificio Departamental de Biología, Campus M. Unamuno, Salamanca, Spain.
authors.forename: Encarna
authors.initials: E
authors.lastname: Velázquez
authors.affiliation: Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain; Unidad Asociada Universidad de Salamanca-CSIC 'Interacción Planta-Microorganismo', Salamanca, Spain. Electronic address: alvaro.peix@csic.es.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Peix
mesh_terms: D020369:Bradyrhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013030:Spain
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer
keywords: Bradyrhizobium; Phylogeny; Spain; Symbiovar; Vigna unguiculata