title: Cecropia pachystachya: a species with expressive in vivo topical anti-inflammatory and in vitro antioxidant effects.
abstract: Cecropia pachystachya is a species traditionally used in Brazil to treat inflammation. This work aims to evaluate the topical anti-inflammatory and antioxidant activities of the methanolic extract of C. pachystachya (CPM) and to perform its chemical fingerprint by HPLC-DAD. The topical anti-inflammatory activity was evaluated using the mouse models of acute ear inflammation induced by croton oil, arachidonic acid, capsaicin, EPP, phenol, and chronic inflammation induced by multiple application of croton oil. The in vitro antioxidant effect of CPM was investigated using DPPH, reducing power, β -carotene bleaching, and TBARS assays. HPLC analysis was performed to quantify the antioxidant phenolics orientin, isoorientin, and chlorogenic acid previously identified in CPM. CPM exhibited significant anti-inflammatory effect in the acute models, in some cases comparable to the reference drugs. Histopathological analysis showed a moderate chronic skin anti-inflammatory effect with decrease in vasodilation, edema, cell infiltration, and epidermal hyperproliferation. It also showed strong in vitro antioxidant activity. The contents of orientin, isoorientin, and chlorogenic acid were 66.5 ± 1.8, 118.8 ± 0.7, and 5.4 ± 0.2 µg/mg extract, respectively. The topical anti-inflammatory activity of CPM could be based on its antioxidant properties, although other effects are probably involved, including COX inhibition and other mechanisms.
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, 36036 900 Juiz de Fora, MG, Brazil.
authors.forename: Natália Ramos
authors.initials: NR
authors.lastname: Pacheco
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, 36036 900 Juiz de Fora, MG, Brazil.
authors.forename: Nícolas de Castro Campos
authors.initials: Nde C
authors.lastname: Pinto
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, 36036 900 Juiz de Fora, MG, Brazil.
authors.forename: Josiane Mello
authors.identifier: 0000-0003-2601-7671
authors.initials: JM
authors.lastname: da Silva
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, 36036 900 Juiz de Fora, MG, Brazil.
authors.forename: Renata de Freitas
authors.initials: Rde F
authors.lastname: Mendes
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, 36036 900 Juiz de Fora, MG, Brazil.
authors.forename: Juliana de Carvalho
authors.initials: Jde C
authors.lastname: da Costa
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, 36036 900 Juiz de Fora, MG, Brazil.
authors.forename: Danielle Maria de Oliveira
authors.initials: DM
authors.lastname: Aragão
authors.affiliation: Department of Morphology, Biological Sciences Institute, Federal University of Juiz de Fora, 36036 900 Juiz de Fora, MG, Brazil.
authors.forename: Maria Christina Marques Nogueira
authors.initials: MC
authors.lastname: Castañon
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, 36036 900 Juiz de Fora, MG, Brazil.
authors.forename: Elita
authors.identifier: 0000-0002-5580-4845
authors.initials: E
authors.lastname: Scio
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D035702:Cecropia Plant / Q000737:chemistry*; D003872:Dermatitis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004427:Ear Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts