title: Influence of carbon-based nanomaterials on lux-bioreporter Escherichia coli.
abstract: The cytotoxic effects of carbon-based nanomaterials are evaluated via the induction of luminescent genetically engineered Escherichia coli bacterial cells. Specifically, two engineered E. coli bacteria strains of DPD2794 and TV1061 were incubated with aqueous dispersion of three carbon allotropes (multi-wall carbon nanotubes (MWCNTs), graphene nanosheets and carbon black nanopowders) with different concentrations and the resulting bioluminescence was recorded at 30°C and 25°C, respectively. The corresponding optical density changes of bacterial cells in the presence of various carbon nanomaterials were recorded as well. Based on these results, E. coli DPD2794 bacterial induction responds to a greater degree than E. coli TV1061 bacteria when exposed to various carbon-based nanomaterials. Finally, the surface morphology of E. coli DPD2794 bacteria cells before and after carbon-based nanomaterials treatment was observed using a field emission scanning electron microscope (FESEM), from which morphological changes from the presence of carbon-based nanomaterials were observed and discussed.
authors.affiliation: Laboratoire de Nanotechnologie et d׳Instrumentation Optique, Institute Charles Delaunay, Université de technologie de Troyes, UMR-CNRS 6281, 12 Rue Marie-Curie CS 42060, 10004 Troyes Cedex, France.
authors.forename: Kun
authors.initials: K
authors.lastname: Jia
authors.affiliation: Department of Biotechnology Engineering, Faculty of Engineering Science, Ben-Gurion University of the Negev, PO Box 653, 84105 Beer-Sheva, Israel; National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, Israel; The Ilse Katz Centre for Meso and Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; School of Materials Science and Engineering, Nanyang Technological University, Singapore 637722, Singapore.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Marks
authors.affiliation: Laboratoire de Nanotechnologie et d׳Instrumentation Optique, Institute Charles Delaunay, Université de technologie de Troyes, UMR-CNRS 6281, 12 Rue Marie-Curie CS 42060, 10004 Troyes Cedex, France. Electronic address: elena_rodica.ionescu@utt.fr.
authors.forename: Rodica E
authors.initials: RE
authors.lastname: Ionescu
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure; D015870:Gene Expression / Q000187:drug effects; D017930:Genes, Reporter / Q000235:genetics; D005818:Genetic Engineering; D006108:Graphite / Q000633:toxicity*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D049449:Luminescence; D049408:Luminescent Agents / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods; D050296:Microbial Viability / Q000187:drug effects / Q000235:genetics; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000633:toxicity* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000633:toxicity*; D009876:Operon / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D053260:Soot / Q000633:toxicity*; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D049408:Luminescent Agents; D037742:Nanotubes, Carbon; D053260:Soot; D006108:Graphite; D010088:Oxidoreductases; D008156:Luciferases; C055198:LuxC protein, Bacteria; D000217:Acyltransferases; C065928:luxD protein, Bacteria
keywords: Bioluminescence; Carbon black nanopowder; Carbon nanotube; Engineered E. coli bacteria; Graphene nanosheet