title: Baculovirus induces host cell aggregation via a Rho/Rok-dependent mechanism.
abstract: Several baculoviruses can induce host cell aggregation during infection; however, the molecular basis remains unknown. The Rho family of small GTPases, including Rho1, Racs and Cdc42, plays important roles in cell migration and cell-cell contact. Activated GTPases target actin polymerization to discrete sites on the plasma membrane, thereby inducing membrane protrusions. In this study, we demonstrated that Spodoptera litura nucleopolyhedrovirus (SpltNPV) infection induced the amoeboid movement and aggregation of SpLi-221 cells in vitro. The amount of Rho1-GTP increased in the infected cells, which suggested that Rho1 was activated upon infection. RNA interference and superinfection of dominant-negative recombinants revealed that the SpltNPV-induced SpLi-221 cell aggregation was dependent on the Rho1, but not Racs or Cdc42, signalling pathway. Inhibition of Rho-associated protein kinase (Rok) activity by the inhibitor Y-27632 significantly reduced SpLi-221 cell aggregation. Silencing Rho1 expression with RNA interference decreased SpltNPV propagation by approximately 40 % in vitro, when SpLi-221 cells were infected at a low, but not high, m.o.i., suggesting that the SpltNPV-induced cell aggregation may benefit SpltNPV spread.
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Zihao
authors.initials: Z
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Meijin
authors.initials: M
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Pang
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000502:physiology*; D002449:Cell Aggregation; D002460:Cell Line; D020868:Gene Silencing; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D018411:Spodoptera / Q000821:virology*; D020741:rho GTP-Binding Proteins / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000378:metabolism*
chemical_list: D054460:rho-Associated Kinases; D020741:rho GTP-Binding Proteins