title: Potential protection of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside against staurosporine-induced toxicity on cultured rat hippocampus neurons.
abstract: The present study explored the effect of 2,3,5,4'-tetrahydroxystilbene-2-O-β-d-glucoside (THSG) on the staurosporine (STS)-induced toxicity in cultured rat hippocampal neurons. The results showed that administration of 200μM of THSG significantly protected against 0.3μM of STS-induced apoptosis in cultured rat hippocampal neurons tested by methyl thiazolyl tetrazolium (MTT) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assays. Furthermore, when the Akt signaling pathway was blocked by LY294002, an inhibitor of Phosphatidyl Inositol 3-kinase (PI3K), the protective effects of THSG against STS-induced neurotoxicity were abrogated. We further examined the involvement of PI3K/Akt signaling pathway in THSG protection against STS-induced cytotoxicity on cultured neurons and found that administration of THSG significantly inhibited the STS-induced decreases in the content of phosphorylated AKt (p-Akt). Moreover, we found that THSG rescued the down-regulation of B cell lymphoma/lewkmia-2 (Bcl2) and pro-caspase-3 (pro-Csp3) caused by STS in the neurons. These results indicate that THSG protect the cultured rat hippocampal neurons against STS-induced cytotoxicity and the PI3K/Akt signaling and mitochondrial apoptotic pathways are involved in the THSG-induced protective effects.
authors.affiliation: Laboratory of Biotechnology and State Key Laboratory of Chinese Ethnic Minority Traditional Medicine, College of Life & Environmental Science, Minzu University of China, Beijing 100081, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Yang
authors.affiliation: Laboratory of Biotechnology and State Key Laboratory of Chinese Ethnic Minority Traditional Medicine, College of Life & Environmental Science, Minzu University of China, Beijing 100081, China.
authors.forename: Tao-Yan
authors.initials: TY
authors.lastname: Liu
authors.affiliation: Laboratory of Biotechnology and State Key Laboratory of Chinese Ethnic Minority Traditional Medicine, College of Life & Environmental Science, Minzu University of China, Beijing 100081, China. Electronic address: zhongsijia01@163.com.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Qin
authors.affiliation: Laboratory of Neurobiology and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China. Electronic address: yulc@pku.edu.cn.
authors.forename: Long-Chuan
authors.initials: LC
authors.lastname: Yu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D061251:Primary Cell Culture; D011498:Protein Precursors / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D019311:Staurosporine / Q000494:pharmacology*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D005960:Glucosides; D018696:Neuroprotective Agents; D011498:Protein Precursors; D019253:Proto-Oncogene Proteins c-bcl-2; D013267:Stilbenes; C093026:2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D053148:Caspase 3; D019311:Staurosporine
keywords: Bcl(2); Hippocampal neurons; STS; THSG; p-Akt; pro-Csp3