title: Effect of N-methyl-D-aspartic acid on activity of superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione level in selected organs of the mouse.
abstract: One of the major classes of ionotropic glutamate receptors is the class of N-methyl-D-aspartate receptors (NMDARs). Receptor activation recruits, via calcium signal transduction mechanisms which play important roles in oxidative metabolism, mitochondrial free radical production and occurrence of other mitochondrial factors which potentially contribute to excitotoxicity and neuronal death. In the present study, the effects of stimulation of NMDARs by applying N-methyl-D-aspartic acid (NMDA) in the brain, liver, kidneys and pancreas on change of the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx) and in the amount of reduced glutathione (GSH) in blood, brain, liver and kidneys has been investigated. Statistically significant decrease of the activity of SOD, CAT and GSHPx and in the amount of reduced glutathione (GSH) was found in the examined organs after administration of NMDA, an agonist of NMDA receptors, demonstrating that NMDA administration compromises the antioxidant status in the investigated organs of the mouse.
authors.affiliation: Pedagogical University of Cracow Department of Animal Physiology and Toxicology, Institute of Biology Podbrzezie 3 31-054 Cracow Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Szaroma
authors.affiliation: Pedagogical University of Cracow Department of Animal Physiology and Toxicology, Institute of Biology Podbrzezie 3 31-054 Cracow Poland.
authors.forename: K
authors.initials: K
authors.lastname: Dziubek
authors.affiliation: Pedagogical University of Cracow Department of Animal Physiology and Toxicology, Institute of Biology Podbrzezie 3 31-054 Cracow Poland.
authors.forename: E
authors.initials: E
authors.lastname: Kapusta
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002374:Catalase / Q000378:metabolism*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D005978:Glutathione / Q000097:blood / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D016202:N-Methylaspartate / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*; D013997:Time Factors
chemical_list: D018690:Excitatory Amino Acid Agonists; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: N-methyl-D-aspartic acid; catalase; glutathione peroxidase; mouse; reduced glutathione; superoxide dismutase