title: A novel VHH antibody targeting the B cell-activating factor for B-cell lymphoma.
abstract: OBJECTIVE
  To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody.
  
  
  METHODS
  Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro.
  
  
  CONCLUSIONS
  The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.
authors.affiliation: Department of Lymphoma, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China. wuwen1981@163.com; Department of Blood Transfusion, Jinan Military General Hospital, Jinan 250031, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wu
authors.affiliation: Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin Shengfa NabioTech Co., Ltd., Tianjin 300457, China. shenghuali@hotmail.com.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Lymphoma, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China. zhangwj3072@163.com.
authors.forename: Weijing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin Shengfa NabioTech Co., Ltd., Tianjin 300457, China. haoyou3003@gmail.com.
authors.forename: Guangda
authors.initials: G
authors.lastname: Ren
authors.affiliation: Division of Science Popularization, China Science and Technology Exchange Center, Beijing 100045, China. dongqc@cstec.org.cn.
authors.forename: Quanchao
authors.initials: Q
authors.lastname: Dong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D053264:B-Cell Activating Factor / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D002161:Camelids, New World; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D016393:Lymphoma, B-Cell / Q000188:drug therapy* / Q000276:immunology; D008969:Molecular Sequence Data; D019151:Peptide Library; D016415:Sequence Alignment; D061905:Single-Domain Antibodies / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*
chemical_list: D053264:B-Cell Activating Factor; D007143:Immunoglobulin Heavy Chains; D019151:Peptide Library; D061905:Single-Domain Antibodies