title: Acorus tatarinowii Schott extract protects PC12 cells from amyloid-beta induced neurotoxicity.
abstract: Amyloid-beta induced neurotoxicity has been identified as a major cause of Alzheimer's disease. Acorus tatarinowii Schott is one of the most frequently used Chinese herbs for Alzheimer's disease treatment. However, the effects of Acorus tatarinowii Schott on amyloid-beta mediated nerve cell damage remains unknown. In the present study, neuronal differentiated PC12 cells were used as a model to evaluate the effects of A. tatarinowii Schott extract (ATSE) against Abeta25-35 induced neurotoxicity. The results showed pretreatment with ATSE significantly protected PC12 cells from Abeta25-35 induced cell death, lactate dehydrogenase release, DNA damage, mitochondrial dysfunction and cytochrome c release from mitochondria. In addition, pretreatment with ATSE also significantly inhibited Abeta25-35 induced caspase-3 activation and reactive oxygen species generation in PC12 cells. These observations suggested that ATSE protects PC12 cells from amyloid-beta induced neurotoxicity.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: An
authors.forename: Guo-Wen
authors.initials: GW
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Lin
authors.forename: Chao
authors.initials: C
authors.lastname: Gu
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.forename: Wen-Xian
authors.initials: WX
authors.lastname: Sun
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Qiu
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
mesh_terms: D029023:Acorus / Q000737:chemistry*; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D018696:Neuroprotective Agents; D009498:Neurotoxins / Q000037:antagonists & inhibitors*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D007202:Indicators and Reagents; D018696:Neuroprotective Agents; D009498:Neurotoxins; D010446:Peptide Fragments; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C074636:amyloid beta-protein (25-35); D007770:L-Lactate Dehydrogenase; D053148:Caspase 3