title: [Expression and significance of miR-125a and Mcl-1 in intestinal tissue after massive small bowel resection in rat].
abstract: OBJECTIVE
  To investigate the expression and significance of miR-125a and anti-apoptotic protein Mcl-1 in intestinal tissue after massive small bowel resection in intestinal adaptation.
  
  
  METHODS
  Sprague-Dawley rats (54 male rats, 8-week old) were divided into 3 groups randomly, including two control groups. Rats in the experiment group were subjected to 70% massive small bowel resection. Rats in the resection group underwent simple intestinal resection and anastomosis. Rats in the control group underwent laparotomy alone. A 5 cm intestine approximately 1 cm distal to the anastomosis was harvested a week after operation. Expression of Mcl-1 was assessed by immunohistochemistry and real-time PCR was used to detect the expression of miR-125a in intestinal tissue.
  
  
  RESULTS
  The positive expression of Mcl-1 in the experiment group was 18.8%(3/16), significantly lower than that in the control group(76.5%, 13/17) and the resection group (83.33%, 15/18)(both P<0.01). The expression of miR-125a in the experiment group was 1.92, significantly higher than that in the control group (1.01) and the resection group (1.05)(both P<0.01).
  
  
  CONCLUSIONS
  miR-125a and anti-apoptotic protein Mcl-1 may play an important role in intestinal adaptation process and they may regulate each other through a certain pathway.
authors.affiliation: Department of Gastrointestinal and Pancreatic Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. majinp@gmail.com.
authors.forename: Mengfei
authors.initials: M
authors.lastname: Xian
authors.forename: Jinping
authors.initials: J
authors.lastname: Ma
authors.forename: Sile
authors.initials: S
authors.lastname: Chen
authors.forename: Jianwei
authors.initials: J
authors.lastname: Lin
authors.forename: Weiling
authors.initials: W
authors.lastname: He
authors.forename: Dongjie
authors.initials: D
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.forename: Chuangqi
authors.initials: C
authors.lastname: Chen
authors.forename: Shirong
authors.initials: S
authors.lastname: Cai
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D004195:Disease Models, Animal; D007421:Intestine, Small / Q000378:metabolism / Q000601:surgery; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012778:Short Bowel Syndrome / Q000378:metabolism*
chemical_list: C581261:MIRN125 microRNA, rat; C579093:Mcl1 protein, rat; D035683:MicroRNAs; D064549:Myeloid Cell Leukemia Sequence 1 Protein