title: Development of asthmatic response upon bronchial allergen challenge is associated with dynamic changes of interleukin-10-producing and interleukin-10-responding CD4+ T cells.
abstract: The role of adaptive immune system in regulation of asthmatic responses remains elusive. Here, we performed a comprehensive time-course analysis of mutual relationships between development of asthmatic response following allergen challenge and changes in several CD4+ T cell subsets which we characterized as either releasing interleukin-10 (CD4+CD25-CD127- and CD4+CD25+CD127+ T cells) or responding to IL-10 (CD4+ T cell subsets expressing CD210). Patients that developed asthmatic reaction were described as responders (R) whereas the others were named non-responders (NR). In R, in contrast to NR, at 6 h, we demonstrated significant expansion of CD4+CD25-CD127- T cells which was followed by drop to baseline values at 24 h. In contrast, in R, we observed decrease in numbers of CD4+CD25+CD127+ and CD4+CD25-CD127+ T cells at 24 h. Interestingly, at baseline, despite comparable IL-10 levels, R presented with lower levels of all CD4+ T cell subsets expressing CD210. In R, the numbers of CD4+CD210+ T cell subsets were further decreased following bronchial challenge which was paralleled by decrease in IL-10 serum levels. Altogether, our data suggest that dynamic interactions between IL-10-producing and IL-10-responding CD4+ T cells could contribute to pathogenesis of asthmatic responses in atopic individuals.
authors.affiliation: Department of Allergology and Internal Medicine, Medical University of Bialystok, Sklodowskiej-Curie 24A, 15-276, Bialystok, Poland, Marcin.Moniuszko@umb.edu.pl.
authors.forename: Marcin
authors.initials: M
authors.lastname: Moniuszko
authors.forename: Kamil
authors.initials: K
authors.lastname: Grubczak
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kowal
authors.forename: Andrzej
authors.initials: A
authors.lastname: Eljaszewicz
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Rusak
authors.forename: Marta
authors.initials: M
authors.lastname: Jeznach
authors.forename: Ewa
authors.initials: E
authors.lastname: Jablonska
authors.forename: Milena
authors.initials: M
authors.lastname: Dabrowska
authors.forename: Anna
authors.initials: A
authors.lastname: Bodzenta-Lukaszyk
mesh_terms: D000280:Administration, Inhalation; D000485:Allergens / Q000008:administration & dosage*; D039741:Antigens, Dermatophagoides / Q000008:administration & dosage*; D001249:Asthma / Q000097:blood* / Q000139:chemically induced / Q000175:diagnosis; D015415:Biomarkers / Q000097:blood; D001985:Bronchial Provocation Tests / Q000379:methods*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D015415:Biomarkers; C508609:IL10 protein, human; D016753:Interleukin-10