title: Association of tissue mRNA and serum antigen levels of members of the urokinase-type plasminogen activator system with clinical and prognostic parameters in prostate cancer.
abstract: The objective was to determine the mRNA expression and protein levels of uPA system components in tissue specimens and serum samples, respectively, from prostate cancer (PCa) patients and to assess their association with clinicopathological parameters and overall survival (OS). The mRNA expression levels of uPA, its receptor (uPAR), and its inhibitor type 1 (PAI-1) were analyzed in corresponding malignant and adjacent nonmalignant tissue specimens from 132 PCa patients by quantitative PCR. Preoperative serum samples from 81 PCa patients were analyzed for antigen levels of uPA system members by ELISA. RNA levels of uPA system components displayed significant correlations with each other in the tumor tissues. A significantly decreased uPA mRNA expression in PCa compared to the corresponding nonmalignant tissue was detected. High uPA mRNA level was significantly associated with a high Gleason score. Elevated concentration of soluble uPAR (suPAR) in serum was significantly associated with a poor OS of PCa patients (P = 0.022). PCa patients with high suPAR levels have a significantly higher risk of death (multivariate Cox's regression analysis; HR = 7.12, P = 0.027). The association of high suPAR levels with poor survival of PCa patients suggests a prognostic impact of suPAR levels in serum of cancer patients.
authors.affiliation: Urologische Klinik, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, 91052 Erlangen, Germany.
authors.forename: Omar
authors.initials: O
authors.lastname: Al-Janabi
authors.affiliation: Urologische Klinik, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, 91052 Erlangen, Germany.
authors.forename: Helge
authors.initials: H
authors.lastname: Taubert
authors.affiliation: Klinik für Urologie, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Lohse-Fischer
authors.affiliation: Klinik für Urologie, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Fröhner
authors.affiliation: Urologische Klinik, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, 91052 Erlangen, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Wach
authors.affiliation: Pathologisches Institut, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Stöhr
authors.affiliation: Urologische Klinik, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, 91052 Erlangen, Germany.
authors.forename: Bastian
authors.initials: B
authors.lastname: Keck
authors.affiliation: Department of Urology and Pediatric Urology, Julius-Maximilians-University, 97070 Würzburg, Germany.
authors.forename: Max
authors.initials: M
authors.lastname: Burger
authors.affiliation: Clinic of Urology, Caritas-Hospital St. Josef, 93053 Regensburg, Germany.
authors.forename: Wolf
authors.initials: W
authors.lastname: Wieland
authors.affiliation: Klinik für Urologie, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Kati
authors.initials: K
authors.lastname: Erdmann
authors.affiliation: Klinik für Urologie, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Manfred P
authors.initials: MP
authors.lastname: Wirth
authors.affiliation: Urologische Klinik, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, 91052 Erlangen, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Wullich
authors.affiliation: Institut für Pathologie, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Baretton
authors.affiliation: Department of Obstetrics and Gynecology, Technical University of Munich, 81675 Munich, Germany.
authors.forename: Viktor
authors.initials: V
authors.lastname: Magdolen
authors.affiliation: Institut für Pathologie, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Kotzsch
authors.affiliation: Klinik für Urologie, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Füssel
mesh_terms: D000368:Aged; D000951:Antigens, Neoplasm / Q000097:blood*; D018572:Disease-Free Survival; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood*; D011467:Prostate / Q000378:metabolism* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism*; D055293:Receptors, Urokinase Plasminogen Activator / Q000097:blood*; D015996:Survival Rate; D014568:Urokinase-Type Plasminogen Activator / Q000097:blood*
chemical_list: D000951:Antigens, Neoplasm; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D055293:Receptors, Urokinase Plasminogen Activator; C051321:SERPINE1 protein, human; D014568:Urokinase-Type Plasminogen Activator