title: Identification and characterization of the grape WRKY family.
abstract: WRKY transcription factors have functions in plant growth and development and in response to biotic and abiotic stresses. Many studies have focused on functional identification of WRKY transcription factors, but little is known about the molecular phylogeny or global expression patterns of the complete WRKY family. In this study, we identified 80 WRKY proteins encoded in the grape genome. Based on the structural features of these proteins, the grape WRKY genes were classified into three groups (groups 1-3). Analysis of WRKY genes expression profiles indicated that 28 WRKY genes were differentially expressed in response to biotic stress caused by grape whiterot and/or salicylic acid (SA). In that 16 WRKY genes upregulated both by whiterot pathogenic bacteria and SA. The results indicated that 16 WRKY proteins participated in SA-dependent defense signal pathway. This study provides a basis for cloning genes with specific functions from grape.
authors.affiliation: Forestry Department, Agricultural University of Henan Province, Zhengzhou, Henan 450002, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Forestry Department, Agricultural University of Henan Province, Zhengzhou, Henan 450002, China.
authors.forename: Jian Can
authors.initials: JC
authors.lastname: Feng
mesh_terms: D000595:Amino Acid Sequence; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D005810:Multigene Family / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification; D027843:Vitis / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors