title: Genetic diversity and virulence potential of shiga toxin-producing Escherichia coli O113:H21 strains isolated from clinical, environmental, and food sources.
abstract: Shiga toxin-producing Escherichia coli strains of serotype O113:H21 have caused severe human diseases, but they are unusual in that they do not produce adherence factors coded by the locus of enterocyte effacement. Here, a PCR microarray was used to characterize 65 O113:H21 strains isolated from the environment, food, and clinical infections from various countries. In comparison to the pathogenic strains that were implicated in hemolytic-uremic syndrome in Australia, there were no clear differences between the pathogens and the environmental strains with respect to the 41 genetic markers tested. Furthermore, all of the strains carried only Shiga toxin subtypes associated with human infections, suggesting that the environmental strains have the potential to cause disease. Most of the O113:H21 strains were closely related and belonged in the same clonal group (ST-223), but CRISPR analysis showed a great degree of genetic diversity among the O113:H21 strains.
authors.affiliation: Division of Microbiology, Food and Drug Administration, College Park, Maryland, USA peter.feng@fda.hhs.gov.
authors.forename: Peter C H
authors.initials: PC
authors.lastname: Feng
authors.affiliation: Laboratory for Food Safety, French Agency for Food, Environmental, and Occupational Health and Safety, Maisons-Alfort, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Delannoy
authors.affiliation: Division of Molecular Biology, Food and Drug Administration, Laurel, Maryland, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Lacher
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Luis Fernando
authors.initials: LF
authors.lastname: Dos Santos
authors.affiliation: National Reference Laboratory for Escherichia coli, Federal Institute for Risk Assessment, Berlin, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Beutin
authors.affiliation: Laboratory for Food Safety, French Agency for Food, Environmental, and Occupational Health and Safety, Maisons-Alfort, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Fach
authors.affiliation: Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas-ANLIS "Dr. Carlos G. Malbrán," Buenos Aires, Argentina.
authors.forename: Marta
authors.initials: M
authors.lastname: Rivas
authors.affiliation: Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Hartland
authors.affiliation: Research Centre for Infectious Diseases, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, Australia.
authors.forename: Adrienne W
authors.initials: AW
authors.lastname: Paton
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Beatriz E C
authors.initials: BE
authors.lastname: Guth
mesh_terms: D000818:Animals; D002417:Cattle; D004285:Dogs; D004783:Environmental Microbiology; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D014644:Genetic Variation; D006041:Goats; D006801:Humans; D008460:Meat / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D022621:Shiga Toxin / Q000378:metabolism; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D037521:Virulence Factors / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors; D022621:Shiga Toxin