title: PirB-Cry2Aa hybrid protein exhibits enhanced insecticidal activity against Spodoptera exigua larvae.
abstract: Previous study revealed that the N-terminal region of PirB toxin from Photorhabdus luminescens showed 20.5% identity and 41.5% similarity to the domain I of Cry2A toxin of Bacillus thuringiensis. The encoding sequence of the domain I of Cry2Aa protein was replaced by the encoding gene of corresponding domain of PirB protein. Expression of pirB-cry2Aa chimeric gene in B. thuringiensis acrystalliferous strain Cry(-)B leads to the formation of crystals with irregular shape. Bioassay showed that PirB-Cry2Aa hybrid protein displayed toxicity against Spodoptera exigua and Helicoverpa armigera larvae. Our data implied that PirB protein might possess pore-forming activity and PirB-Cry2Aa hybrid protein could be used as biological control agent.
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Hu
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China.
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Ding
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China.
authors.forename: Liqiu
authors.initials: L
authors.lastname: Xia
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China. Electronic address: hsbalp@163.com.
authors.forename: Shengbiao
authors.initials: S
authors.lastname: Hu
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000633:toxicity*; D004731:Endotoxins / Q000633:toxicity*; D006460:Hemolysin Proteins / Q000235:genetics / Q000633:toxicity*; D007814:Larva / Q000382:microbiology; D010572:Pest Control, Biological / Q000379:methods*; D020637:Photorhabdus; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000633:toxicity*; D018411:Spodoptera / Q000382:microbiology*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011993:Recombinant Fusion Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Bacillus thuringiensis; Domain crystal; Photorhabdus luminescens; Recombineering