title: Bordetella pertussis lipid A glucosamine modification confers resistance to cationic antimicrobial peptides and increases resistance to outer membrane perturbation.
abstract: Bordetella pertussis, the causative agent of whooping cough, has many strategies for evading the human immune system. Lipopolysaccharide (LPS) is an important Gram-negative bacterial surface structure that activates the immune system via Toll-like receptor 4 and enables susceptibility to cationic antimicrobial peptides (CAMPs). We show modification of the lipid A region of LPS with glucosamine increased resistance to numerous CAMPs, including LL-37. Furthermore, we demonstrate that this glucosamine modification increased resistance to outer membrane perturbation.
authors.affiliation: Department of Microbiology and Immunology, The University of British Columbia, Vancouver, Canada.
authors.forename: Nita R
authors.initials: NR
authors.lastname: Shah
authors.affiliation: Department of Microbiology and Immunology, The University of British Columbia, Vancouver, Canada.
authors.forename: Robert E W
authors.initials: RE
authors.lastname: Hancock
authors.affiliation: Department of Microbiology and Immunology, The University of British Columbia, Vancouver, Canada rachelf@mail.ubc.ca.
authors.forename: Rachel C
authors.initials: RC
authors.lastname: Fernandez
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D001886:Bordetella pertussis / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D005944:Glucosamine / Q000737:chemistry / Q000378:metabolism*; D008050:Lipid A / Q000737:chemistry / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011113:Polymyxins / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D008050:Lipid A; D011113:Polymyxins; D005944:Glucosamine