title: Superinfection exclusion reveals heteroimmunity between Pseudomonas aeruginosa temperate phages.
abstract: Temperate siphophages (MP29, MP42, and MP48) were isolated from the culture supernatant of clinical Pseudomonas aeruginosa isolates. The complete nucleotide sequences and annotation of the phage genomes revealed the overall synteny to the known temperate P. aeruginosa phages such as MP22, D3112, and DMS3. Genome-level sequence analysis showed the conservation of both ends of the linear genome and the divergence at the previously identified dissimilarity regions (R1 to R9). Protein sequence alignment of the c repressor (ORF1) of each phage enabled us to divide the six phages into two groups: D3112 group (D3112, MP29, MP42, and MP48) and MP22 group (MP22 and DMS3). Superinfection exclusion was observed between the phages belonging to the same group, which was mediated by the specific interaction between the c repressor and the cognate operator. Based on these, we suggest that the temperate siphophages prevalent in the clinical strains of P. aeruginosa represent at least two distinct heteroimmunity groups.
authors.affiliation: Department of Pharmacy, College of Pharmacy, CHA University, Gyeonggi-do, 463-840, Republic of Korea.
authors.forename: In-Young
authors.initials: IY
authors.lastname: Chung
authors.forename: Hee-Won
authors.initials: HW
authors.lastname: Bae
authors.forename: Hye-Jung
authors.initials: HJ
authors.lastname: Jang
authors.forename: Bi-o
authors.initials: BO
authors.lastname: Kim
authors.forename: You-Hee
authors.initials: YH
authors.lastname: Cho
mesh_terms: D017105:Pseudomonas Phages / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D015163:Superinfection / Q000276:immunology*