title: Candida zeylanoides as a new yeast model for lipid metabolism studies: effect of nitrogen sources on fatty acid accumulation.
abstract: Lipid homeostasis is well-known in oleaginous yeasts, but there are few non-oleaginous yeast models apart from Saccharomyces cerevisiae. We are proposing the non-oleaginous yeast Candida zeylanoides QU 33 as model. The aim of this study was to investigate the influence of the carbon/nitrogen ratio and the type of nitrogen source upon oil accumulation by this yeast grown on shake flask cultures. The maximum biomass was obtained in yeast extract (2.39 ± 0.19 g/l), followed by peptone (2.24 ± 0.05 g/l), while the highest content of microbial oil (0.35 ± 0.01 g/l) and the maximum lipid yield (15.63%) were achieved with peptone. Oleic acid was the predominant cellular fatty acid in all culture media (>32.23%), followed by linoleic (>15.79%) and palmitic acids (>13.47%). The highest lipid yield using glucose and peptone was obtained at the C/N ratio of 200:1.
authors.affiliation: Programa de Pós-graduação em Microbiologia Agrícola e do Meio Ambiente (PPGMAA), Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil, pri_dr_rosa@hotmail.com.
authors.forename: Priscila Dallé
authors.initials: PD
authors.lastname: da Rosa
authors.forename: Paula
authors.initials: P
authors.lastname: Mattanna
authors.forename: Diórgenes
authors.initials: D
authors.lastname: Carboni
authors.forename: Lucio
authors.initials: L
authors.lastname: Amorim
authors.forename: Neila
authors.initials: N
authors.lastname: Richards
authors.forename: Patricia
authors.initials: P
authors.lastname: Valente
mesh_terms: D002175:Candida / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D050356:Lipid Metabolism; D009584:Nitrogen / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D009584:Nitrogen