title: A fluorescent switch for sequentially and selectively sensing copper(II) and L-histidine in vitro and in living cells.
abstract: Herein, we report the development of a new fluorescent switch for sequential and selective sensing of Cu(2+) and L-histidine (L-His) in vitro and in living cells for the first time. In the absence of metal ions, Ac-SAACQ-Gly-Gly-Gly-Lys (FITC) (1) exhibits comparable fluorescence to that of free FITC. In the presence of metal ions, 1 selectively coordinates to Cu(2+) , causing its fluorescence emission to be quenched via photoinduced electron transfer. Interestingly, the as-formed 1–Cu(2+) complex selectively responds to L-His among the 20 natural amino acids by turning its fluorescence on. This property of fluorescence switch of 1 was successfully applied for qualitatively and quantitatively sensing Cu(2+) and L-His in vitro. Using this dual functional probe, we also sequentially imaged Cu(2+) and L-His in living HepG2 cells. Our new probe 1 could be applied for not only environmental monitoring but also biomolecule detection in the near future.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry & Collaborative Innovation Center of Suzhou Nano Science and Technology, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China. gliang@ustc.edu.cn.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Wang
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Miao
authors.forename: Tingjie
authors.initials: T
authors.lastname: Song
authors.forename: Qingpan
authors.initials: Q
authors.lastname: Yuan
authors.forename: Jinhao
authors.initials: J
authors.lastname: Gao
authors.forename: Gaolin
authors.initials: G
authors.lastname: Liang
mesh_terms: D002413:Cations, Divalent / Q000032:analysis; D056831:Coordination Complexes / Q000737:chemistry; D003300:Copper / Q000032:analysis*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006639:Histidine / Q000032:analysis*; D006801:Humans; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D061848:Optical Imaging; D013050:Spectrometry, Fluorescence
chemical_list: D002413:Cations, Divalent; D056831:Coordination Complexes; D005456:Fluorescent Dyes; D009842:Oligopeptides; D006639:Histidine; D003300:Copper; C035647:glycyl-glycyl-glycine; D016650:Fluorescein-5-isothiocyanate