title: Dose response effect of Paracoccidioides brasiliensis in an experimental model of arthritis.
abstract: Paracoccidioidomycosis (PCM) is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb) and corresponds to prevalent systemic mycosis in Latin America. The aim of the present work was to evaluate the dose response effect of the fungal yeast phase for the standardization of an experimental model of septic arthritis. The experiments were performed with groups of 14 rats that received doses of 103, 104 or 105 P. brasiliensis (Pb18) cells. The fungi were injected in 50 µL of phosphate-buffered saline (PBS) directly into the knee joints of the animals. The following parameters were analyzed in this work: the formation of swelling in knees infused with yeast cells and the radiological and anatomopathological alterations, besides antibody titer by ELISA. After 15 days of infection, signs of inflammation were evident. At 45 days, some features of damage and necrosis were observed in the articular cartilage. The systemic dissemination of the fungus was observed in 11% of the inoculated animals, and it was concluded that the experimental model is able to mimic articular PCM in humans and that the dose of 105 yeast cells can be used as standard in this model.
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, PR, Brazil.
authors.forename: Eduardo Alexandre
authors.initials: EA
authors.lastname: Loth
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, PR, Brazil.
authors.forename: Samia Khalil
authors.initials: SK
authors.lastname: Biazim
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, PR, Brazil.
authors.forename: José Henrique Fermino Ferreira
authors.initials: JH
authors.lastname: Dos Santos
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Rosana
authors.initials: R
authors.lastname: Puccia
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, PR, Brazil.
authors.forename: Rosimeire Costa
authors.initials: RC
authors.lastname: Brancalhão
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, PR, Brazil.
authors.forename: Lucinéia de Fátima
authors.initials: Lde F
authors.lastname: Chasco
authors.affiliation: Centro de Ciências Médicas e Farmacêuticas, Hospital Universitário do Oeste do Paraná, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil.
authors.forename: Rinaldo Ferreira
authors.initials: RF
authors.lastname: Gandra
authors.affiliation: Centro de Ciências Médicas e Farmacêuticas, Hospital Universitário do Oeste do Paraná, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil.
authors.forename: Rita de Cássia Garcia
authors.initials: Rde C
authors.lastname: Simão
authors.affiliation: Departamento de Patologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Marcello Fabiano
authors.initials: MF
authors.lastname: de Franco
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000382:microbiology* / Q000473:pathology; D001170:Arthritis, Infectious / Q000382:microbiology* / Q000473:pathology; D001175:Arthrography; D006651:Histocytochemistry; D008297:Male; D010228:Paracoccidioides / Q000472:pathogenicity*; D010229:Paracoccidioidomycosis / Q000382:microbiology* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar