title: Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system.
abstract: Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.
authors.affiliation: 1] Biology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France. [2] CNRS URA 2581, Institut Pasteur, Paris, France. [3].
authors.forename: Mehdi
authors.initials: M
authors.lastname: Ghorbal
authors.affiliation: 1] Biology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France. [2] CNRS URA 2581, Institut Pasteur, Paris, France.
authors.forename: Molly
authors.initials: M
authors.lastname: Gorman
authors.affiliation: 1] Biology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France. [2] CNRS URA 2581, Institut Pasteur, Paris, France.
authors.forename: Cameron Ross
authors.initials: CR
authors.lastname: Macpherson
authors.affiliation: 1] Biology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France. [2] CNRS URA 2581, Institut Pasteur, Paris, France.
authors.forename: Rafael Miyazawa
authors.initials: RM
authors.lastname: Martins
authors.affiliation: 1] Biology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France. [2] CNRS URA 2581, Institut Pasteur, Paris, France.
authors.forename: Artur
authors.initials: A
authors.lastname: Scherf
authors.affiliation: 1] Biology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France. [2] CNRS URA 2581, Institut Pasteur, Paris, France. [3].
authors.forename: Jose-Juan
authors.initials: JJ
authors.lastname: Lopez-Rubio
mesh_terms: D000818:Animals; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D018503:Genome, Protozoan; D010963:Plasmodium falciparum / Q000235:genetics*