title: High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis-advantages and limitations.
abstract: Chromatography techniques such as HPTLC and HPLC are commonly used to produce a chemical fingerprint of a plant to allow identification and quantify the main constituents within the plant. The aims of this study were to compare HPTLC and HPLC, for qualitative and quantitative analysis of the major constituents of Calendula officinalis and to investigate the effect of different extraction techniques on the C. officinalis extract composition from different parts of the plant. The results found HPTLC to be effective for qualitative analysis, however, HPLC was found to be more accurate for quantitative analysis. A combination of the two methods may be useful in a quality control setting as it would allow rapid qualitative analysis of herbal material while maintaining accurate quantification of extract composition.
authors.affiliation: School of Pharmacy and Applied Science, La Trobe Institute of Molecular Sciences, La Trobe University, Edwards Rd, Bendigo 3550, VIC, Australia.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Loescher
authors.affiliation: School of Pharmacy and Applied Science, La Trobe Institute of Molecular Sciences, La Trobe University, Edwards Rd, Bendigo 3550, VIC, Australia.
authors.forename: David W
authors.initials: DW
authors.lastname: Morton
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia.
authors.forename: Slavica
authors.initials: S
authors.lastname: Razic
authors.affiliation: School of Pharmacy and Applied Science, La Trobe Institute of Molecular Sciences, La Trobe University, Edwards Rd, Bendigo 3550, VIC, Australia. Electronic address: s.kustrin@latrobe.edu.au.
authors.forename: Snezana
authors.initials: S
authors.lastname: Agatonovic-Kustrin
mesh_terms: D020838:Calendula / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control
chemical_list: D010936:Plant Extracts
keywords: Calendula; Fingerprint profiling; HPLC; HPTLC; Quality control