title: A highly rapid and simple competitive enzyme-linked immunosorbent assay for monitoring paralytic shellfish poisoning toxins in shellfish.
abstract: Using a streptavidin-coated well plate, a biotin-labelled anti-gonyautoxin 2/3 monoclonal antibody GT-13A, and a decarbamoyl saxitoxin-peroxidase conjugate, a direct competitive enzyme-linked immunosorbent assay (PSP-ELISA) was developed for monitoring paralytic shellfish poisoning (PSP) toxins in shellfish. This assay is simple to perform and can be completed in approximately 20 min. The PSP-ELISA was compared to the mouse bioassay (MBA) for the detection of PSP toxins in shellfish samples (n=83) collected from the coast of Osaka Prefecture, Japan. When positive and negative results were indicated based on the regulatory limit for PSP toxins (4 mouse unit(MU)/g of shellfish meat), the PSP-ELISA results showed a sensitivity of 100% (25 of 25) and a specificity of 89.7% (52 of 58 samples) compared to the MBA results. These results suggest that the PSP-ELISA could be used as a rapid and simple screening method prior to the MBA.
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan. Electronic address: kawatu@iph.pref.osaka.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kawatsu
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kanki
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Harada
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kumeda
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D051379:Mice; D012758:Shellfish / Q000009:adverse effects* / Q000032:analysis; D057096:Shellfish Poisoning / Q000175:diagnosis*
keywords: Direct competitive ELISA; Monitoring; Mouse bioassay; Paralytic shellfish poisoning toxins