title: A novel and effective strategy for the isolation of adipose-derived stem cells: minimally manipulated adipose-derived stem cells for more rapid and safe stem cell therapy.
abstract: Adipose-derived stem cells are an ideal mesenchymal stem cell population for regenerative medical application. The isolation procedure is performed by mechanical isolation under a laminar air flow bench without using serum or animal-derived reagents; cells were characterized by flow cytometric analysis. Cell availability is improved compared with enzymatic digestion procedures. The adipose-derived stem cell mechanical isolating procedure presented here is easier, safer, cheaper, and faster than traditional currently performed enzymatic procedures.
authors.affiliation: Parma, Italy From the Department of Surgical Sciences, Plastic Surgery Division, University of Parma; and the Department of Clinical and Experimental Medicine, Division of Hematology, Parma University Hospital.
authors.forename: Edoardo
authors.initials: E
authors.lastname: Raposio
authors.forename: Giorgia
authors.initials: G
authors.lastname: Caruana
authors.forename: Sabrina
authors.initials: S
authors.lastname: Bonomini
authors.forename: Guido
authors.initials: G
authors.lastname: Libondi
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D015187:Lipectomy; D059630:Mesenchymal Stem Cells / Q000166:cytology*