title: [Identification of small RNA in polyhedra of Bombyx mori nuclear polyhedrosis virus].
abstract: It has been shown by bioinformatic methods that regions of the Bombyx mori viral nuclear polyhedrosis genome encoded two small RNA--snc RNA-1 and snc RNA-2, which could perform a structural function in polyhedra crystals formation. The aim of this work was identification of the nucleotide sequence of small non-coding RNAs, predicted by bioinformatic methods in B. mori polyhedra. The following methods have been used: polymerase chain reaction, agarose gel electrophoresis, the cloning of PCR products, sequencing. There were first determined nucleotide sequences of snc RNA-1 and snc RNA-2 ofpolyhedrin mRNA complementary regions which are included in B. mori polyhedra. These RNAs have 100% identity with bioinformatic predicted sequences. These results confirmed our bioinformatic approach to the search for small RNAs encoded in B. mori nuclear polyhedrosis virus genome.
authors.forename: T V
authors.initials: TV
authors.lastname: Shirina
authors.forename: A V
authors.initials: AV
authors.lastname: Gerashchenko
authors.forename: M T
authors.initials: MT
authors.lastname: Bobrovskaia
authors.forename: V I
authors.initials: VI
authors.lastname: Kashuba
mesh_terms: D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000821:virology; D003001:Cloning, Molecular; D019295:Computational Biology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000737:chemistry / Q000235:genetics*; D000077183:Occlusion Body Matrix Proteins; D016133:Polymerase Chain Reaction; D058727:RNA, Small Untranslated / Q000737:chemistry* / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D000077183:Occlusion Body Matrix Proteins; D058727:RNA, Small Untranslated; D012367:RNA, Viral; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus