title: Genetic polymorphisms of interleukin-1 beta and osteosarcoma risk.
abstract: OBJECTIVE
  Osteosarcoma is the most common childhood bone cancer. Interleukin-1 beta (IL-1B) is crucially involved in osteosarcoma carcinogenesis. Whether genetic polymorphisms of IL-1B also influence osteosarcoma risk is unknown. The aim of this study was to investigate the association between IL-1B gene polymorphisms and osteosarcoma risk in Chinese Han patients.
  
  
  METHODS
  A hospital-based case-control study involving 120 osteosarcoma patients and 120 controls was conducted. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis was performed to detect three IL-1B gene polymorphisms (-31 T/C, -511 C/T and +3954 C/T) in these patients.
  
  
  RESULTS
  Patients with osteosarcoma had a significantly lower frequency of -31 CC genotype [odds ratio (OR) = 0.40, 95% confidence interval (CI) = 0.17-0.92; P = 0.03] and -31 C allele (OR = 0.67, 95% CI = 0.46-0.99; P = 0.04) than controls. Patients with osteosarcoma had a significantly lower frequency of -511 TT genotype (OR = 0.40, 95% CI = 0.17-0.95; P = 0.04) than controls. The +3954 C/T gene polymorphisms were not associated with a risk of osteosarcoma. When stratified by Enneking stage, tumour location, histological type, tumour metastasis of osteosarcoma and family history of cancer, no statistically significant results were found.
  
  
  CONCLUSIONS
  This is the first study to provide evidence for an association of IL-1B gene polymorphisms with osteosarcoma risk.
authors.affiliation: Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, 430022, Hubei Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: He
authors.forename: XinJun
authors.initials: X
authors.lastname: Liang
authors.forename: ChunQing
authors.initials: C
authors.lastname: Meng
authors.forename: ZengWu
authors.initials: Z
authors.lastname: Shao
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wu
authors.forename: JianXiang
authors.initials: J
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: ShuHua
authors.initials: S
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D044466:Asian People / Q000235:genetics; D001859:Bone Neoplasms / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D012516:Osteosarcoma / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D015995:Prevalence; D012307:Risk Factors; D055815:Young Adult
chemical_list: D053583:Interleukin-1beta