title: Effects of aflatoxin B1 exposure and sodium selenite supplementation on the histology, cell proliferation, and cell cycle of jejunum in broilers.
abstract: The aim of this study was to investigate the effects of aflatoxin B1 (AFB1) exposure and sodium selenite supplementation on the histology, cell proliferation and cell cycle of jejunum in broilers. A total of 240 1-day-old male AA broilers were divided into four groups of 60 each, fed with basal diet (control group), 0.3 mg/kg AFB1 (AFB1 group), 0.4 mg/kg supplement Se (Se group), and 0.3 mg/kg AFB1 + 0.4 mg/kg supplement Se (AFB1 + Se group) for 21 days, respectively. Compared with the control group, decreased jejunal villus height, villus height/crypt ratio, and proliferation cell nuclear antigen (PCNA)-positive cells, and G2/M phase arrest and shedded epithelial cells on the tip of jejunal villus were observed in AFB1 groups at 7 and 14 days of age. However, the villus/crypt ratio, PCNA-positive cells and cell percentage of G0/G1, S, and G2/M phases had no significant differences between AFB1 group and control group at 21 days. Simultaneous supplementation with sodium selenite restored these parameters to be close to those in control group. In conclusion, 0.3 mg/kg AFB1 in the diet inhibits the development of broiler's jejunum by reducing cellular proliferation and inducing G2/M arrest during only the first 2 weeks after hatching. Supplementation of dietary sodium selenite at the concentration of 0.4 mg/kg Se had protective action against these toxic effects of AFB1.
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China.
authors.forename: Shengqiang
authors.initials: S
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Chen
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002645:Chickens / Q000502:physiology*; D019587:Dietary Supplements; D007583:Jejunum / Q000166:cytology* / Q000187:drug effects*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D018038:Sodium Selenite / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects
chemical_list: D000975:Antioxidants; D018809:Proliferating Cell Nuclear Antigen; D016604:Aflatoxin B1; D018038:Sodium Selenite