title: A clip-domain serine proteinase homolog (SPH) in oriental river prawn, Macrobrachium nipponense provides insights into its role in innate immune response.
abstract: In this study, a clip-domain serine proteinase homolog designated as MnSPH was cloned and characterized from a freshwater prawn Macrobrachium nipponense. The full-length cDNA of MnSPH was 1897 bp and contained a 1701 bp open reading frame (ORF) encoding a protein of 566 amino acids, a 103 bp 5'-untranslated region, and a 93 bp 3'-untranslated region. Sequence comparison showed that the deduced amino acids of MnSPH shared 30-59% identity with sequences reported in other animals. Tissue distribution analysis indicated that the MnSPH transcripts were present in all detected tissues with highest in the hepatopancreas and ovary. The MnSPH mRNA levels in the developing ovary were stable at the initial three developmental stages, then increased gradually from stage IV (later vitellogenesis), and reached a maximum at stage VI (paracmasis). Furthermore, the expression of MnSPH mRNA in hemocytes was significantly up-regulated at 1.5 h, 6 h, 12 h and 48 h post Aeromonas hydrophila injection. The increased phenoloxidase activity also demonstrated a clear time-dependent pattern after A. hydrophila challenge. These results suggest that MnSPH participates in resisting to pathogenic microorganisms and plays a pivotal role in host defense against microbe invasion in M. nipponense.
authors.affiliation: School of Life Science, East China Normal University, Shanghai 200062, PR China; School of Life Science, Huzhou Normal University, Huzhou, Zhejiang 313000, PR China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Ding
authors.affiliation: School of Life Science, East China Normal University, Shanghai 200062, PR China; School of Life Science, Huzhou Normal University, Huzhou, Zhejiang 313000, PR China.
authors.forename: Youqin
authors.initials: Y
authors.lastname: Kong
authors.affiliation: School of Life Science, East China Normal University, Shanghai 200062, PR China. Electronic address: lqchen@bio.ecnu.edu.cn.
authors.forename: Liqiao
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Biological Sciences, Flinders University, Adelaide, SA 5001, Australia.
authors.forename: Jianguang
authors.initials: J
authors.lastname: Qin
authors.affiliation: School of Life Science, East China Normal University, Shanghai 200062, PR China.
authors.forename: Shengming
authors.initials: S
authors.lastname: Sun
authors.affiliation: School of Life Science, East China Normal University, Shanghai 200062, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Life Science, East China Normal University, Shanghai 200062, PR China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Du
authors.affiliation: School of Life Science, Huzhou Normal University, Huzhou, Zhejiang 313000, PR China.
authors.forename: Jinyun
authors.initials: J
authors.lastname: Ye
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004789:Enzyme Activation / Q000276:immunology*; D005260:Female; D006434:Hemocytes / Q000378:metabolism; D007113:Immunity, Innate / Q000276:immunology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010053:Ovary / Q000254:growth & development / Q000378:metabolism; D033541:Palaemonidae / Q000201:enzymology* / Q000382:microbiology; D017422:Sequence Analysis, DNA; D057057:Serine Proteases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D057057:Serine Proteases
keywords: Innate immune; Macrobrachium nipponense; Serine protease homolog; mRNA expression