title: Multispecies biofilm in an artificial wound bed--A novel model for in vitro assessment of solid antimicrobial dressings.
abstract: Wound infections represent a major problem, particularly in patients with chronic wounds. Bacteria in the wound exist mainly in the form of biofilms and are thus resistant to most antibiotics and antimicrobials. A simple and cost-effective in vitro model of chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. The method is based on the well-established Lubbock chronic wound biofilm transferred onto an artificial agar wound bed. The biofilm formed by four bacterial species (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa) was stable for up to 48h post-transplant. The applicability of the model was evaluated by testing two common iodine wound treatments. These observations indicate that this method enables assessing the effects of treatments on established resilient wound biofilms and is clinically highly relevant.
authors.affiliation: Wound Healing Research Group, ContiproPharma, Dolní Dobrouč, Czech Republic; Department of Histology and Embryology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Kucera
authors.affiliation: Cell Physiology Research Group, ContiproBiotech, Dolní Dobrouč, Czech Republic; Institute of Microbiology, Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia. Electronic address: martin.sojka@szu.sk.
authors.forename: M
authors.initials: M
authors.lastname: Sojka
authors.affiliation: Cell Physiology Research Group, ContiproBiotech, Dolní Dobrouč, Czech Republic; Department of Dermatology, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Pavlik
authors.affiliation: Wound Healing Research Group, ContiproPharma, Dolní Dobrouč, Czech Republic.
authors.forename: K
authors.initials: K
authors.lastname: Szuszkiewicz
authors.affiliation: Wound Healing Research Group, ContiproPharma, Dolní Dobrouč, Czech Republic; Cell Physiology Research Group, ContiproBiotech, Dolní Dobrouč, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Velebny
authors.affiliation: Wound Healing Research Group, ContiproPharma, Dolní Dobrouč, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Klein
mesh_terms: D000890:Anti-Infective Agents / Q000008:administration & dosage*; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D058491:Bacterial Load; D001458:Bandages; D018441:Biofilms / Q000187:drug effects*; D002908:Chronic Disease; D015870:Gene Expression; D005798:Genes, Bacterial; D066298:In Vitro Techniques; D007455:Iodine / Q000008:administration & dosage; D010641:Phenotype; D014946:Wound Infection / Q000188:drug therapy* / Q000382:microbiology*
chemical_list: D000890:Anti-Infective Agents; D007455:Iodine
keywords: Anti-biofilm substances; Artificial wound bed; In vitro testing; Multi-species wound biofilm model