title: Comparison of promoter DNA methylation and expression levels of genes encoding CCAAT/enhancer binding proteins in AML patients.
abstract: CCAAT/enhancer binding proteins (CEBPs) are transcription factors regulating myeloid differentiation. Disturbances of their expression may contribute to leukemogenesis. In this study we compared promoter methylation and expression levels of selected CEBP genes in a group of 78 AML patients, normal bone marrow and hematopoietic precursor cells. CEBPA, CEBPD and CEBPE promoter methylation levels were elevated in 37%, 35.5% and 56.7% of patients. No CEBPZ(DDIT3) methylation was observed. An inverse relationship between CEBPA and CEBPD DNA methylation and expression levels was observed. AML cytogenetic risk groups and patients with particular translocation are characterized by distinct methylation/expression profile of CEBPs encoding genes.
authors.affiliation: Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland. Electronic address: musialik.ewa@gmail.com.
authors.forename: Ewa
authors.initials: E
authors.lastname: Musialik
authors.affiliation: Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland. Electronic address: mbujko@coi.waw.pl.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Bujko
authors.affiliation: Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland. Electronic address: pkober@coi.waw.pl.
authors.forename: Paulina
authors.initials: P
authors.lastname: Kober
authors.affiliation: Department of Immunology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland. Electronic address: m.a.grygorowicz@gmail.com.
authors.forename: Monika Anna
authors.initials: MA
authors.lastname: Grygorowicz
authors.affiliation: Department of Hematology, Oncology and Internal Diseases, The Medical University of Warsaw, Warsaw, Poland. Electronic address: marta.libura@gmail.com.
authors.forename: Marta
authors.initials: M
authors.lastname: Libura
authors.affiliation: Department of Hematology, Oncology and Internal Diseases, The Medical University of Warsaw, Warsaw, Poland. Electronic address: m.przest@autograf.pl.
authors.forename: Marta
authors.initials: M
authors.lastname: Przestrzelska
authors.affiliation: Department of Diagnostic Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland. Electronic address: pjuszczynski@ihit.waw.pl.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Juszczyński
authors.affiliation: Department of Diagnostic Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland. Electronic address: kborg@ihit.waw.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Borg
authors.affiliation: Department of Hematology, Jagiellonian University, Cracow, Poland. Electronic address: iflorek@gmail.com.
authors.forename: Izabela
authors.initials: I
authors.lastname: Florek
authors.affiliation: Department of Hematology, Jagiellonian University, Cracow, Poland. Electronic address: jakobcz@tlen.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Jakóbczyk
authors.affiliation: Neuroortopedic Department, Marian Weiss Memorial Mazowia Rehabilitation Center, Konstancin-Jeziorna, Poland. Electronic address: alicja.baranowska@onet.eu.
authors.forename: Alicja
authors.initials: A
authors.lastname: Baranowska
authors.affiliation: Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland. Electronic address: jas@coi.waw.pl.
authors.forename: Janusz Aleksander
authors.initials: JA
authors.lastname: Siedlecki
mesh_terms: D000328:Adult; D000368:Aged; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic; D014178:Translocation, Genetic
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins
keywords: Acute myelogenous leukemia; CCAAT/enhancer binding protein; CEBPA; CEBPD; CEBPE; CEBPZ(DDIT3); DNA methylation; Gene expression