title: The value of new high-throughput technologies for diagnosis and prognosis in solid tumors.
abstract: Advances in our understanding of the molecular basis of tumors, as well as in the technology of DNA analysis, are rapidly changing the landscape of these diseases. Traditional approaches such as sequencing methods and arrays have too many limits. These have been overcome by the advent of next generation sequencing (NGS) methods which facilitate and accelerate the analysis of multiple genes and samples. These technologies allow new applications in molecular biology and medicine, for example precise analysis of RNA transcripts for gene expression; profiling of small RNAs, DNA methylation patterns and histone modification analysis; identification of splicing isoforms and of DNA regions that interact with regulatory proteins; pharmacogenomics studies and so on. In this review we describe recent applications of NGS in genomics, transcriptomics and epigenomics for a better comprehension of solid tumor metabolisms.
authors.affiliation: National Cancer Research Centre "Giovanni Paolo II", Bari, Italy.
authors.forename: Rosamaria
authors.initials: R
authors.lastname: Pinto
authors.affiliation: National Cancer Research Centre "Giovanni Paolo II", Bari, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: De Summa
authors.affiliation: National Cancer Research Centre "Giovanni Paolo II", Bari, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Petriella
authors.affiliation: The Oncology Institute "Prof. Dr. Ion Chiricuta", Cluj-Napoca, Romania.
authors.forename: Oana
authors.initials: O
authors.lastname: Tudoran
authors.affiliation: National Cancer Research Centre "Giovanni Paolo II", Bari, Italy.
authors.forename: Katia
authors.initials: K
authors.lastname: Danza
authors.affiliation: National Cancer Research Centre "Giovanni Paolo II", Bari, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Tommasi
mesh_terms: D019175:DNA Methylation; D057890:Epigenomics; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D057170:Translational Research, Biomedical
chemical_list: D006657:Histones; D035683:MicroRNAs
keywords: Next generation sequencing; genomics; transcriptomics and epigenomics