title: A food additive with prebiotic properties of an α-d-glucan from lactobacillus plantarum DM5.
abstract: An α-d-glucan produced by Lactobacillus plantarum DM5 was explored for in vitro prebiotic activities. Glucan-DM5 demonstrated 21.6% solubility, 316.9% water holding capacity, 86.2% flocculation activity, 71.4% emulsification activity and a degradation temperature (Td) of 292.2°C. Glucan-DM5 exhibited lowest digestibility of 0.54% by artificial gastric juice, 0.21% by intestinal fluid and 0.32% by α-amylase whereas the standard prebiotic inulin, showed 25.23%, 5.97% and 19.13%, hydrolysis, respectively. Prebiotic activity assay of glucan-DM5 displayed increased growth of probiotic bacteria such as Bifidobacterium infantis and Lactobacillus acidophilus, but did not support the growth of non-probiotic bacteria such as Escherichia coli and Enterobacter aerogenes. The overall findings indicated that glucan from L. plantarum DM5 can serve as a potential prebiotic additive for food products.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, India.
authors.forename: Deeplina
authors.initials: D
authors.lastname: Das
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, India.
authors.forename: Rwivoo
authors.initials: R
authors.lastname: Baruah
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, India. Electronic address: arungoyl@iitg.ernet.in.
authors.forename: Arun
authors.initials: A
authors.lastname: Goyal
mesh_terms: D032701:Biomimetics; D045703:Emulsifying Agents / Q000737:chemistry; D005427:Flocculation; D005503:Food Additives / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D019649:Food Industry; D005750:Gastric Juice / Q000378:metabolism; D005936:Glucans / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006868:Hydrolysis; D048191:Lactiplantibacillus plantarum / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development; D056692:Prebiotics; D012995:Solubility; D014867:Water / Q000737:chemistry; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D045703:Emulsifying Agents; D005503:Food Additives; D005936:Glucans; D056692:Prebiotics; D014867:Water; D000516:alpha-Amylases
keywords: Glucan; Inulin; Lactobacillus plantarum; Marcha; Prebiotic