title: Comparative evaluation of serum, FTA filter-dried blood and oral fluid as sample material for PRRSV diagnostics by RT-qPCR in a small-scale experimental study.
abstract: Recently, research into alternative sample materials, such as oral fluid or filter-dried blood has been intensified, in order to facilitate cost-effective and animal-friendly sampling of individuals or groups of pigs for diagnostic purposes. The objective of this study was to compare the sensitivity of porcine reproductive and respiratory syndrome virus (PRRSV)-RNA detection by reverse transcription quantitative real-time PCR (RT-qPCR) in serum, FTA filter-dried blood and oral fluid sampled from individual pigs. Ten PRRSV negative pigs were injected with an EU-type PRRSV live vaccine. Blood and oral fluid samples were taken from each pig before, and 4, 7, 14 and 21 days after vaccination. All samples were then analyzed by PRRSV RT-qPCR. In serum, eight often pigs tested RT-qPCR positive at different time points post infection. Absolute quantification showed low serum PRRSV-RNA loads in most samples. In comparison to serum, sensitivity of PRRSV-RNA detection was strongly reduced in matched FTA filter-dried blood and in oral fluid from the same pigs. These results indicate that with low PRRSV-RNA loads the diagnostic sensitivity of PRRSV-RNA detection by RT-qPCR achieved with serum is currently unmatched by either FTA filter-dried blood or oral fluid.
authors.forename: Adolf
authors.initials: A
authors.lastname: Steinrigl
authors.forename: Sandra
authors.initials: S
authors.lastname: Revilla-Fernández
authors.forename: Eveline
authors.initials: E
authors.lastname: Wodak
authors.forename: Friedrich
authors.initials: F
authors.lastname: Schmoll
authors.forename: Tatjana
authors.initials: T
authors.lastname: Sattler
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards / Q000662:veterinary*; D003362:Cost-Benefit Analysis; D059788:Dried Blood Spot Testing / Q000592:standards / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary; D005260:Female; D008297:Male; D019318:Porcine Reproductive and Respiratory Syndrome / Q000097:blood / Q000175:diagnosis*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000097:blood / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012463:Saliva / Q000821:virology*; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral