title: Development and evaluation of new primers for PCR-based identification of Prevotella intermedia.
abstract: The aim of this study was to develop new Prevotella intermedia-specific PCR primers based on the 16S rRNA. The new primer set, Pi-192 and Pi-468, increased the accuracy of PCR-based P. intermedia identification and could be useful in the detection of P. intermedia as well as epidemiological studies on periodontal disease.
authors.affiliation: Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, 639 Zhi Zao Ju Road, Shanghai 210011, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, 639 Zhi Zao Ju Road, Shanghai 210011, China.
authors.forename: Dali
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, 639 Zhi Zao Ju Road, Shanghai 210011, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 210011, China.
authors.forename: Cailian
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Endodontics and Operative Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 210011, China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, 639 Zhi Zao Ju Road, Shanghai 210011, China. Electronic address: shurong123@hotmail.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Shu
mesh_terms: D016866:Bacteroidaceae Infections / Q000175:diagnosis*; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010510:Periodontal Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D018713:Prevotella intermedia / Q000235:genetics* / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Prevotella intermedia; Species-specific PCR primers