title: Separating the isomers--efficient synthesis of the N-hydroxysuccinimide esters of 5 and 6-carboxyfluorescein diacetate and 5 and 6-carboxyrhodamine B.
abstract: Diacetate protection of 5 and 6-carboxyfluorescein followed by synthesis of the N-hydroxysuccinimide esters allowed ready separation of the two isomers on a multi-gram scale. The 5 and 6-carboxyrhodamine B N-hydroxysuccinimide esters were also readily synthesised and separated.
authors.affiliation: School of Chemistry, EaStChem, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Brunet
authors.affiliation: School of Chemistry, EaStChem, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK.
authors.forename: Tashfeen
authors.initials: T
authors.lastname: Aslam
authors.affiliation: School of Chemistry, EaStChem, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK. Electronic address: mark.bradley@ed.ac.uk.
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
mesh_terms: D004952:Esters / Q000138:chemical synthesis* / Q000302:isolation & purification; D005452:Fluoresceins / Q000737:chemistry*; D015394:Molecular Structure; D012235:Rhodamines / Q000737:chemistry*; D013237:Stereoisomerism; D013388:Succinimides / Q000138:chemical synthesis* / Q000302:isolation & purification
chemical_list: D004952:Esters; D005452:Fluoresceins; D012235:Rhodamines; D013388:Succinimides; C093616:6-carboxyfluorescein diacetate; C084577:5-carboxyfluorescein diacetate; C001426:N-hydroxysuccinimide
keywords: 5(6)-Carboxyfluorescein; Carboxyfluorescein diacetate; N-Hydroxysuccinimide ester; Rhodamine B