title: Induction of stemlike cells with fibrogenic properties by carbon nanotubes and its role in fibrogenesis.
abstract: We developed a three-dimensional fibroblastic nodule model for fibrogenicity testing of nanomaterials and investigated the role of fibroblast stemlike cells (FSCs) in the fibrogenic process. We showed that carbon nanotubes (CNTs) induced fibroblastic nodule formation in primary human lung fibroblast cultures resembling the fibroblastic foci in clinical fibrosis and promoted FSCs that are highly fibrogenic and a potential driving force of fibrogenesis. This study provides a predictive 3D model and mechanistic insight on CNT fibrogenesis.
authors.affiliation: Pharmaceutical and Pharmacological Sciences Program, ‡Mary Babb Randolph Cancer Center, §Animal Models and Imaging Facility, and ∥Department of Chemical Engineering, West Virginia University , Morgantown, West Virginia 26506, United States.
authors.forename: Sudjit
authors.initials: S
authors.lastname: Luanpitpong
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
authors.forename: Amruta
authors.initials: A
authors.lastname: Manke
authors.forename: Karen H
authors.initials: KH
authors.lastname: Martin
authors.forename: Amanda Gatesman
authors.initials: AG
authors.lastname: Ammer
authors.forename: Vincent
authors.initials: V
authors.lastname: Castranova
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Yon
authors.initials: Y
authors.lastname: Rojansakul
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D008954:Models, Biological; D037742:Nanotubes, Carbon / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon