title: Biocontrol traits and antagonistic potential of Bacillus amyloliquefaciens strain NJZJSB3 against Sclerotinia sclerotiorum, a causal agent of canola stem rot.
abstract: Bacillus amyloliquefaciens strain NJZJSB3 has shown antagonism of several phytopathogens in vitro, especially Sclerotinia sclerotiorum. Both the broth culture and cell suspension of strain NJZJSB3 could completely protect the detached leaves of canola (Brassica napus) from S. sclerotiorum infection. In pot experiments, the application of strain NJZJSB3 cell suspension (10(8) CFU/ml) decreased the disease incidence by 83.3%, a result similar to commercially available fungicide (Dimetachlone). In order to investigate the potential biocontrol mechanisms of strain NJZJSB3, the nonvolatile antifungal compounds it produces were identified as iturin homologs using HPLC-ESI-MS. Antifungal volatile organic compounds were identified by gas chromatography-mass spectrometry. The detected volatiles toluene, phenol, and benzothiazole showed antifungal effects against S. sclerotiorum in chemical control experiments. Strain NJZJSB3 also produced biofilm, siderophores and cell-wall-degrading enzymes (protease and β-1,3-glucanase). These results suggest that strain NJZJSB3 can be a tremendous potential agent for the biological control of sclerotinia stem rot.
authors.affiliation: National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, Nanjing 210095, P. R. China.
authors.forename: Yuncheng
authors.initials: Y
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Yuan
authors.forename: Waseem
authors.initials: W
authors.lastname: Raza
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000898:Antibiosis; D000935:Antifungal Agents / Q000032:analysis / Q000302:isolation & purification; D001203:Ascomycota / Q000254:growth & development*; D001407:Bacillus / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development; D029688:Brassica napus / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D010572:Pest Control, Biological / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000935:Antifungal Agents