title: Facile synthesis of multilayered polysaccharidic vesicles.
abstract: In this study, we developed facile synthesis method of multilayered polysaccharidic vesicles (hereafter termed 'mPSVs') using polysaccharides such as starch, hyaluronate (HA), and glycol chitosan (GC) via simple chemistry and using enzymatic reactions among polysaccharides. The enzymatic degradation of the HA shell by hyaluronidase (HYAL) enzyme contributed to accelerate the release of protein/peptide from the mPSVs. The mPSVs containing folate ligand and apoptotic cell death-inducing D-(KLAKLAK)2 peptide were effectively accumulated in in vivo KB tumor cells, primarily owing to passive tumor penetration via the enhanced permeability and retention (EPR) effect and active targeting via specific binding to folate receptors expressed on KB tumor cells. These mPSVs resulted in a significant increase in the in vivo tumor inhibition. This vesicle system is expected to exhibit great potential as an advanced platform technology for biomedical applications involving small molecular drugs with protein/gene targets.
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok 2-dong, Wonmi-gu, Bucheon, Gyeonggi-do 420-743, Republic of Korea.
authors.forename: Dong Sup
authors.initials: DS
authors.lastname: Kwag
authors.affiliation: College of Pharmacy, Chung-Ang University, 221 Heukseok dong, Dongjak-gu, Seoul 155-756, Republic of Korea.
authors.forename: Kyung Taek
authors.initials: KT
authors.lastname: Oh
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok 2-dong, Wonmi-gu, Bucheon, Gyeonggi-do 420-743, Republic of Korea. Electronic address: eslee@catholic.ac.kr.
authors.forename: Eun Seong
authors.initials: ES
authors.lastname: Lee
mesh_terms: D000681:Amylases / Q000008:administration & dosage / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry*; D005260:Female; D005492:Folic Acid / Q000737:chemistry; D058972:Folic Acid Transporters / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008819:Mice, Nude; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry; D013213:Starch / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D058972:Folic Acid Transporters; D010455:Peptides; C118638:glycol-chitosan; D011107:Polylysine; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; D013213:Starch; D048271:Chitosan; D005492:Folic Acid; D000681:Amylases
keywords: Multilayered vesicle; Peptide/protein drug; Polypeptide; Polysaccharide