title: Periodontal disease and high doses of inhaled corticosteroids alter NTPDase activity in the blood serum of rats.
abstract: BACKGROUND
  Certain drugs such as glucocorticoids may interfere with the modulation of periodontal disease. In contrast, corticosteroid treatment has been associated with a protective effect with regard to periodontal breakdown, depending on the dose, pathway, and exposure time. Considering the potential relevance of nucleotidases in coordinating the cardiovascular system and inflammation processes, the aim of this study was to investigate the nucleotidase activities in the blood serum of rats with periodontal disease exposed chronically to inhaled corticosteroids.
  
  
  METHODS
  Adult male Wistar rats (n=26) were randomly assigned to one of the following four study groups: a control group that received no intervention; a periodontal disease group that received saline solution; a 'low dose' group that received 30 μg of budesonide daily; and a corresponding 'high dose' group that received 100 μg daily over a 15-day time course. The hydrolysis of ATP, ADP, and AMP were analysed in blood serum.
  
  
  RESULTS
  Periodontal disease diminished the hydrolysis of ATP and enhanced the hydrolysis of ADP. Repeated administration of either a low or high dose in the periodontal disease model of inhaled corticosteroids reversed the observed increase in ADP hydrolysis, and only the repeated administration of low doses of inhaled corticosteroids was able to reverse the decrease in the hydrolysis of ATP induced by periodontal disease.
  
  
  CONCLUSIONS
  The variables investigated in this study may be involved in the pathophysiology of periodontal disease and may participate in the mechanisms that mediate the development of some of the side effects of inhaled corticosteroids.
authors.affiliation: Laboratório de Farmacologia da Dor e Neuromodulação: modelos animais - Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Vanessa L
authors.initials: VL
authors.lastname: Scarabelot
authors.affiliation: Programa de Pós-Graduação em Odontologia, Faculdade de Odontologia - UFRGS, Porto Alegre, Brazil.
authors.forename: Juliano
authors.initials: J
authors.lastname: Cavagni
authors.affiliation: Laboratório de Farmacologia da Dor e Neuromodulação: modelos animais - Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Liciane F
authors.initials: LF
authors.lastname: Medeiros
authors.affiliation: Laboratório de Farmacologia da Dor e Neuromodulação: modelos animais - Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Bernardo
authors.initials: B
authors.lastname: Detânico
authors.affiliation: Laboratório de Farmacologia da Dor e Neuromodulação: modelos animais - Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Joanna R
authors.initials: JR
authors.lastname: Rozisky
authors.affiliation: Laboratório de Farmacologia da Dor e Neuromodulação: modelos animais - Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Andressa
authors.initials: A
authors.lastname: de Souza
authors.affiliation: Programa de Pós-Graduação em Odontologia, Faculdade de Odontologia - UFRGS, Porto Alegre, Brazil.
authors.forename: Luciana Dondonis
authors.initials: LD
authors.lastname: Daudt
authors.affiliation: Programa de Pós-Graduação em Odontologia, Faculdade de Odontologia - UFRGS, Porto Alegre, Brazil.
authors.forename: Eduardo José
authors.initials: EJ
authors.lastname: Gaio
authors.affiliation: Departamento de Farmacologia, ICBS, UFRGS, Porto Alegre, Brazil.
authors.forename: Maria Beatriz Cardoso
authors.initials: MB
authors.lastname: Ferreira
authors.affiliation: Programa de Pós-Graduação em Odontologia, Faculdade de Odontologia - UFRGS, Porto Alegre, Brazil.
authors.forename: Cassiano Kuchenbecker
authors.initials: CK
authors.lastname: Rösing
authors.affiliation: Departamento de Bioquímica, ICBS, UFRGS, Porto Alegre, Brazil.
authors.forename: Ana Maria O
authors.initials: AM
authors.lastname: Battastini
authors.affiliation: Laboratório de Farmacologia da Dor e Neuromodulação: modelos animais - Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil; Departamento de Farmacologia, ICBS, UFRGS, Porto Alegre, Brazil. Electronic address: iracitorres@gmail.com.
authors.forename: Iraci L S
authors.initials: IL
authors.lastname: Torres
mesh_terms: D000244:Adenosine Diphosphate / Q000097:blood*; D000249:Adenosine Monophosphate / Q000097:blood*; D000255:Adenosine Triphosphate / Q000097:blood*; D000280:Administration, Inhalation; D000818:Animals; D019819:Budesonide / Q000008:administration & dosage / Q000494:pharmacology*; D006868:Hydrolysis; D008297:Male; D010510:Periodontal Diseases / Q000097:blood* / Q000188:drug therapy*; D011897:Random Allocation; D017208:Rats, Wistar
chemical_list: D000249:Adenosine Monophosphate; D019819:Budesonide; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate
keywords: Inhaled corticosteroid; Periodontal disease; Purinergic signalling; Rats