title: F-01A, an antibiotic, inhibits lung cancer cells proliferation.
abstract: OBJECTIVE
  In an effort to identify novel, small molecules which can affect the proliferation of lung cancer cells, F-01A, a polyether antibiotic isolated from the fermentation broth of Streptomyces was tested.
  
  
  METHODS
  F-01A was tested for its antitumor properties on the lung cancer cell line SPC-A-1, at six doses (0.1, 0.5, 1, 2.5, and 5 μmol·L(-1)), using various cellular assays. Cell viability was measured by the MTT assay, Hochest 33258 was used to study nuclear morphology; DNA ladder and the loss of mitochondrial membrane potential were also evaluated.
  
  
  RESULTS
  F-01A induces apoptosis against SPC-A-1 cells in a dose-dependent manner. The IC50 is 0.65 μmol·L(-1), and the inhibition at 5 μmol·L(-1) is 87.89%. Further, JC-1 staining indicates F-01A could induce the loss of mitochondrial membrane potential, and the DNA fragment is evident.
  
  
  CONCLUSIONS
  Mechanistic analysis showed that F-01A induced apoptosis of cancer cells probably in the mitochondrial pathway. The antitumor actions of F-01A involve activation of the apoptotic pathway against SPC-A-1 cells, and it may be valuable for further drug development.
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, China; College of Life Sciences, Hainan Normal University, Haikou 571158, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Wu
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, China.
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Song
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, China.
authors.forename: Chang-Ri
authors.initials: CR
authors.lastname: Han
authors.affiliation: College of Life Sciences, Hainan Normal University, Haikou 571158, China. Electronic address: zh.chen@hainnu.edu.cn.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, China. Electronic address: chgying123@163.com.
authors.forename: Guang-Ying
authors.initials: GY
authors.lastname: Chen
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000503:physiopathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D006131:Growth Inhibitors
keywords: Apoptosis; Membrane potential; Polyether antibiotic; SPC-A-1 cells