title: The possible advantages of cryoprecipitate prepared from fresh frozen plasma from blood stored for 24 hours.
abstract: BACKGROUND
  To compare the coagulation-factor profile of cryoprecipitate produced from fresh frozen plasma from whole blood (WB) stored for 24 hours at room temperature (24CP) with that of standard cryoprecipitate (CP).
  
  
  METHODS
  We collected 80 units of WB from healthy volunteers, of which 20 units were of each blood group. Each unit of blood was divided into 2 parts. One part was used for preparation and quality-control evaluation of CP within 8 hours of collection; the other part was stored at room temperature for 24 hours and then subjected to CP preparation. Coagulation studies were carried out on each batch of CP after production. Fibrinogen, Factor VIII (FVII), and von Willibrand factor (vWF) were measured, and the blood groups were determined. We used the Student's t-test to perform comparisons and considered results to be significant at P < .005.
  
  
  RESULTS
  Overall, all 3 clotting factors were increased in 24CP compared with CP, with a statistically significant increase in the level of FVIII. Blood group AB had significantly increased levels of fibrinogen and vWF in 24CP compared with CP.
  
  
  CONCLUSIONS
  Our study showed that 24CP has equal or greater levels of coagulation factors compared with CP. His indicates that our alternate approach for preparation of CP may enable more efficient use of blood collected in satellite blood collection centers and during blood drives.
authors.forename: Joseph
authors.initials: J
authors.lastname: Philip
authors.forename: Samantha
authors.initials: S
authors.lastname: Kumarage
authors.forename: Tathagata
authors.initials: T
authors.lastname: Chatterjee
authors.forename: Sudeep
authors.initials: S
authors.lastname: Kumar
authors.forename: Rajeev
authors.initials: R
authors.lastname: Mallhi
mesh_terms: D001780:Blood Coagulation Tests; D001789:Blood Group Antigens; D001793:Blood Preservation; D015925:Cryopreservation; D005169:Factor VIII / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry*; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D011786:Quality Control; D013997:Time Factors; D014841:von Willebrand Factor / Q000737:chemistry*
chemical_list: D001789:Blood Group Antigens; D014841:von Willebrand Factor; D005169:Factor VIII; D005340:Fibrinogen