title: Differential regulation of microRNA transcriptome in chicken lines resistant and susceptible to necrotic enteritis disease.
abstract: Necrotic enteritis (NE) is a re-emerging disease as a result of increased restriction on the use of antibiotics in poultry. However, the molecular mechanisms underlying the pathogenesis of NE are unclear. Small RNA transcriptome analysis was performed using spleen and intestinal intraepithelial lymphocytes (IEL) from 2 inbred chicken lines selected for resistance or susceptibility to Marek's disease (MD) in an experimentally induced model of avian NE to investigate whether microRNA (miRNA) control the expression of genes associated with host response to pathogen challenge. Unique miRNA represented only 0.02 to 0.04% of the total number of sequences obtained, of which 544 were unambiguously identified. Hierarchical clustering revealed that most of miRNA in IEL were highly expressed in the MD-susceptible line 7.2 compared with MD-resistant line 6.3. Reduced CXCL14 gene expression was correlated with differential expression of several unique miRNA in MD-resistant chickens, whereas TGFβR2 gene expression was correlated with altered gga-miR-216 miRNA levels in MD-susceptible animals. In conclusion, miRNA profiling and deep sequencing of small RNA in experimental models of infectious diseases may be useful for further understanding of host-pathogen interactions, and for providing insights into genetic markers of disease resistance.
authors.affiliation: Department of Animal Science and Technology Chung-Ang University, Anseong, 456-756 Republic of Korea yhong@cau.ac.kr.
authors.forename: Yeong Ho
authors.initials: YH
authors.lastname: Hong
authors.affiliation: Department of Animal Science and Technology Chung-Ang University, Anseong, 456-756 Republic of Korea.
authors.forename: Hue
authors.initials: H
authors.lastname: Dinh
authors.affiliation: Animal Biosciences and Biotechnology Laboratory (ABBL), Agricultural Research Services (ARS), USDA, Beltsville, MD 20705.
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.affiliation: Genomic Informatics Center, Hankyong National University, Anseong, 456-749 Republic of Korea.
authors.forename: Ki-Duk
authors.initials: KD
authors.lastname: Song
authors.affiliation: Genomic Informatics Center, Hankyong National University, Anseong, 456-749 Republic of Korea.
authors.forename: Jae-Don
authors.initials: JD
authors.lastname: Oh
mesh_terms: D000818:Animals; D002645:Chickens; D003015:Clostridium Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D003016:Clostridium perfringens / Q000502:physiology; D003048:Coccidiosis / Q000235:genetics / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000502:physiology; D004751:Enteritis / Q000235:genetics / Q000382:microbiology / Q000469:parasitology / Q000662:veterinary; D059014:High-Throughput Nucleotide Sequencing / Q000662:veterinary; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology / Q000469:parasitology; D008214:Lymphocytes / Q000378:metabolism / Q000382:microbiology / Q000469:parasitology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009336:Necrosis / Q000235:genetics / Q000382:microbiology / Q000469:parasitology / Q000662:veterinary; D010802:Phylogeny; D011201:Poultry Diseases / Q000235:genetics* / Q000382:microbiology / Q000469:parasitology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013154:Spleen / Q000378:metabolism / Q000382:microbiology / Q000469:parasitology; D059467:Transcriptome
chemical_list: D035683:MicroRNAs
keywords: Clostridium perfringens; Eimeria maxima; chicken; microRNA; necrotic enteritis; next-generation sequence