title: Determination of grain protein content by near-infrared spectrometry and multivariate calibration in barley.
abstract: Grain protein content (GPC) is an important quality determinant in barley. This research aimed to explore the relationship between GPC and diffuse reflectance spectra in barley. The results indicate that normalizing, and taking first-order derivatives can improve the class models by enhancing signal-to-noise ratio, reducing baseline and background shifts. The most accurate and stable models were obtained with derivative spectra for GPC. Three multivariate calibrations including least squares support vector machine regression (LSSVR), partial least squares (PLS), and radial basis function (RBF) neural network were adopted for development of GPC determination models. The Lin_LSSVR and RBF_LSSVR models showed higher accuracy than PLS and RBF_NN models. Thirteen spectral wavelengths were found to possess large spectrum variation and show high contribution to calibration models. From the present study, the calibration models of GPC in barley were successfully developed and could be applied to quality control in malting, feed processing, and breeding selection.
authors.affiliation: Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Lin
authors.affiliation: Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Chen
authors.affiliation: Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jian
authors.affiliation: Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Shi
authors.affiliation: Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China. Electronic address: jinxl@zju.edu.cn.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Jin
authors.affiliation: Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China. Electronic address: zhanggp@zju.edu.cn.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
mesh_terms: D002138:Calibration; D002523:Edible Grain; D001467:Hordeum / Q000737:chemistry*; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
keywords: Barley (Hordeum vulgare L.); Grain protein content (GPC); Least squares support vector machine regression (LSSVR); Near-infrared spectroscopy (NIRS)