title: Comparative proteomic analysis reveals the suppressive effects of dietary high glucose on the midgut growth of silkworm.
abstract: The silkworm, Bombyx mori, is an important model of lepidoptera insect, and it has been used for several models of human diseases. In human being, long-term high-sugar diet can induce the occurrence of diabetes and other related diseases. Interestingly, our experiments revealed the high glucose diet also has a suppressive effect on the development of silkworms. To investigate the molecular mechanism by which high-glucose diet inhibited the midgut growth in silkworms, we employed comparative proteomic analysis to globally identify proteins differentially expressed in normal and high-glucose diet group silkworms. In all, 28 differently proteins were suppressed and 5 proteins induced in high-glucose diet group. Gene ontology analysis showed that most of these differently proteins are mainly involved in metabolic process, catalytic and cellular process. A development related protein, imaginal disk growth factor (IDGF), was further confirmed by western blot exclusively expressing in the normal diet group silkworms. Taken together, our data suggests that IDGF plays a critical role in impairing the development of silkworms by a high-glucose diet.
  
  
  CONCLUSIONS
  Glucose has been thought to play essential roles in growth and development of silkworm. In this paper, we certified firstly that high-glucose diet can suppress the growth of silkworm, and comparative proteomic was employed to reveal the inhibition mechanism. Moreover, an important regulation related protein (IDGF) was found to involve in this inhibition process. These results will help us get a deeper understanding of the relationship between diet and healthy. Furthermore, IDGF may be the critical protein for reducing the blood sugar in silkworm, and it may be used for screening human hypoglycemic drug. The work has not been submitted elsewhere for publication, in whole or in part, and all the authors have approved the manuscript.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301# Xuefu Road, Zhenjiang, Jiangsu Province 212013, PR China; Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, Jiangsu Province 212013, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Feng
authors.affiliation: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, Jiangsu Province 212013, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301# Xuefu Road, Zhenjiang, Jiangsu Province 212013, PR China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Lian
authors.affiliation: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, Jiangsu Province 212013, PR China.
authors.forename: Hengchuan
authors.initials: H
authors.lastname: Xia
authors.affiliation: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, Jiangsu Province 212013, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, Jiangsu Province 212013, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301# Xuefu Road, Zhenjiang, Jiangsu Province 212013, PR China; Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, Jiangsu Province 212013, PR China. Electronic address: kpchen@ujs.edu.cn.
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D019476:Insect Proteins / Q000096:biosynthesis* / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D040901:Proteomics; D013549:Sweetening Agents / Q000494:pharmacology*
chemical_list: D004040:Dietary Carbohydrates; D019476:Insect Proteins; D013549:Sweetening Agents; D005947:Glucose
keywords: Diet; Glucose; Proteomic; Silkworm; Western blot