title: Uptake and metabolism of D-glucose in isolated acinar and ductal cells from rat submandibular glands.
abstract: The present study deals with the possible effects of selected environmental agents upon the uptake and metabolism of d-glucose in isolated acinar and ductal cells from the rat submandibular salivary gland. In acinar cells, the uptake of d-[U-(14) C]glucose and its non-metabolised analogue 3-O-[(14) C-methyl]-d-glucose was not affected significantly by phloridzin (0.1 mM) or substitution of extracellular NaCl (115 mM) by an equimolar amount of CsCl, whilst cytochalasin B (20 μM) decreased significantly such an uptake. In ductal cells, both phloridzin and cytochalasin B decreased the uptake of d-glucose and 3-O-methyl-d-glucose. Although the intracellular space was comparable in acinar and ductal cells, the catabolism of d-glucose (2.8 or 8.3 mM) was two to four times higher in ductal cells than in acinar cells. Phloridzin (0.1 mM), ouabain (1.0 mM) and cytochalasin B (20 μM) all impaired d-glucose catabolism in ductal cells. Such was also the case in ductal cells incubated in the absence of extracellular Ca(2+) or in media in which NaCl was substituted by CsCl. It is proposed that the ductal cells in the rat submandibular gland are equipped with several systems mediating the insulin-sensitive, cytochalasin B-sensitive and phloridzin-sensitive transport of d-glucose across the plasma membrane.
authors.affiliation: Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Sibel
authors.initials: S
authors.lastname: Cetik
authors.forename: Aigun
authors.initials: A
authors.lastname: Rzajeva
authors.forename: Emeline
authors.initials: E
authors.lastname: Hupkens
authors.forename: Willy J
authors.initials: WJ
authors.lastname: Malaisse
authors.forename: Abdullah
authors.initials: A
authors.lastname: Sener
mesh_terms: D019325:3-O-Methylglucose / Q000378:metabolism; D061354:Acinar Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000737:chemistry; D002478:Cells, Cultured; D002586:Cesium / Q000633:toxicity; D002712:Chlorides / Q000633:toxicity; D003571:Cytochalasin B / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000378:metabolism*; D010042:Ouabain / Q000633:toxicity; D010695:Phlorhizin / Q000494:pharmacology; D051381:Rats; D013363:Submandibular Gland / Q000166:cytology* / Q000187:drug effects
chemical_list: D002250:Carbon Radioisotopes; D002712:Chlorides; D019325:3-O-Methylglucose; D002586:Cesium; D003571:Cytochalasin B; D010042:Ouabain; D010695:Phlorhizin; C028019:cesium chloride; D005947:Glucose; D002118:Calcium
keywords: 3-O-[14C-methyl]-d-glucose or d-[U-14C]glucose handling; d-glucose metabolism; isolated acinar and ductal cells