title: Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications.
abstract: In mammals, the linker histone H1, involved in DNA packaging into chromatin, is represented by a family of variants. H1 tails undergo post-translational modifications (PTMs) that can be detected by mass spectrometry. We developed antibodies to analyze several of these as yet unexplored PTMs including the combination of H1.4 K26 acetylation or trimethylation and S27 phosphorylation. H1.2-T165 phosphorylation was detected at S and G2/M phases of the cell cycle and was dispensable for chromatin binding and cell proliferation; while the H1.4-K26 residue was essential for proper cell cycle progression. We conclude that histone H1 PTMs are dynamic over the cell cycle and that the recognition of modified lysines may be affected by phosphorylation of adjacent residues.
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Catalonia, Spain.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Terme
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Catalonia, Spain.
authors.forename: Lluís
authors.initials: L
authors.lastname: Millán-Ariño
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Catalonia, Spain.
authors.forename: Regina
authors.initials: R
authors.lastname: Mayor
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Catalonia, Spain.
authors.forename: Neus
authors.initials: N
authors.lastname: Luque
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Catalonia, Spain.
authors.forename: Andrea
authors.initials: A
authors.lastname: Izquierdo-Bouldstridge
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Catalonia, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Bustillos
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Catalonia, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Sampaio
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Catalonia, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Canes
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Catalonia, Spain.
authors.forename: Isaura
authors.initials: I
authors.lastname: Font
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Catalonia, Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Sima
authors.affiliation: Centro de Investigación Príncipe Felipe, Valencia, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Sancho
authors.affiliation: Institut de Medecina Predictiva i Personalitzada del Cancer, Badalona, Catalonia, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Torrente
authors.affiliation: Institut de Medecina Predictiva i Personalitzada del Cancer, Badalona, Catalonia, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Forcales
authors.affiliation: Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Roque
authors.affiliation: Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
authors.forename: Pere
authors.initials: P
authors.lastname: Suau
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Catalonia, Spain. Electronic address: albert.jordan@ibmb.csic.es.
authors.forename: Albert
authors.initials: A
authors.lastname: Jordan
mesh_terms: D000107:Acetylation; D045744:Cell Line, Tumor; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational
chemical_list: D006657:Histones
keywords: Histone H1 variants; Linker histone; Post-translational modifications