title: A solvent resistant lab-on-chip platform for radiochemistry applications.
abstract: The application of microfluidics to the synthesis of Positron Emission Tomography (PET) tracers has been explored for more than a decade. Microfluidic benefits such as superior temperature control have been successfully applied to PET tracer synthesis. However, the design of a compact microfluidic platform capable of executing a complete PET tracer synthesis workflow while maintaining prospects for commercialization remains a significant challenge. This study uses an integral system design approach to tackle commercialization challenges such as the material to process compatibility with a path towards cost effective lab-on-chip mass manufacturing from the start. It integrates all functional elements required for a simple PET tracer synthesis into one compact radiochemistry platform. For the lab-on-chip this includes the integration of on-chip valves, on-chip solid phase extraction (SPE), on-chip reactors and a reversible fluid interface while maintaining compatibility with all process chemicals, temperatures and chip mass manufacturing techniques. For the radiochemistry device it includes an automated chip-machine interface enabling one-move connection of all valve actuators and fluid connectors. A vial-based reagent supply as well as methods to transfer reagents efficiently from the vials to the chip has been integrated. After validation of all those functional elements, the microfluidic platform was exemplarily employed for the automated synthesis of a Gastrin-releasing peptide receptor (GRP-R) binding the PEGylated Bombesin BN(7-14)-derivative ([(18)F]PESIN) based PET tracer.
authors.affiliation: GE Global Research, Freisinger Landstrasse 50, 85748 Garching bei Munich, Germany. rensch@ge.com.
authors.forename: Christian
authors.initials: C
authors.lastname: Rensch
authors.forename: Simon
authors.initials: S
authors.lastname: Lindner
authors.forename: Ruben
authors.initials: R
authors.lastname: Salvamoser
authors.forename: Stephanie
authors.initials: S
authors.lastname: Leidner
authors.forename: Christoph
authors.initials: C
authors.lastname: Böld
authors.forename: Victor
authors.initials: V
authors.lastname: Samper
authors.forename: David
authors.initials: D
authors.lastname: Taylor
authors.forename: Marko
authors.initials: M
authors.lastname: Baller
authors.forename: Stefan
authors.initials: S
authors.lastname: Riese
authors.forename: Peter
authors.initials: P
authors.lastname: Bartenstein
authors.forename: Carmen
authors.initials: C
authors.lastname: Wängler
authors.forename: Björn
authors.initials: B
authors.lastname: Wängler
mesh_terms: D001839:Bombesin / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D049268:Positron-Emission Tomography / Q000295:instrumentation / Q000379:methods; D011849:Radioactive Tracers; D011854:Radiochemistry / Q000295:instrumentation / Q000379:methods; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry; D018004:Receptors, Bombesin / Q000737:chemistry
chemical_list: D011849:Radioactive Tracers; D019275:Radiopharmaceuticals; D018004:Receptors, Bombesin; D001839:Bombesin