title: Identification of serum proteome components associated with progression of non-small cell lung cancer.
abstract: The aim of the present study was to perform comparative analysis of serum from patients with different stages of non-small cell lung cancer (NSCLC) using the three complementary proteomic approaches to identify proteome components associated with the progression of cancer. Serum samples were collected before any treatment from 200 patients with NSCLC, including 103 early stage, 64 locally advanced and 33 metastatic cancer samples, and from 200 donors without malignancy. The low-molecular-weight fraction of serum proteome was MALDI-profiled in all samples. Serum proteins were characterized using 2D-PAGE and LC-MS/MS approaches in a representative group of 30 donors. Several significant differences were detected between serum samples collected from patients with early stage cancer and patients with locally advanced cancer, as well as between patients with metastatic cancer and patients with local disease. Of note, serum components discriminating samples from early stage cancer and healthy persons were also detected. In general, about 70 differentiating serum proteins were identified, including inflammatory and acute phase proteins already reported to be associated with the progression of lung cancer (serum amyloid A or haptoglobin). Several differentiating proteins, including apolipoprotein H or apolipoprotein A1, were not previously associated with NSCLC. No significant differences in patterns of serum proteome components were detected between patients with adenocarcinoma and squamous cell carcinoma. In conclusion, we identified the biomarker candidates with potential importance for molecular proteomic staging of NSCLC. Additionally, several serum proteome components revealed their potential applicability in early detection of the lung cancer.
authors.affiliation: Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Pietrowska
authors.affiliation: Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland and Polish-Japanese Institute of Information Technology, Bytom, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Jelonek
authors.affiliation: Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
authors.forename: Malwina
authors.initials: M
authors.lastname: Michalak
authors.affiliation: Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland and Polish-Japanese Institute of Information Technology, Bytom, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Roś
authors.affiliation: Polish Academy of Science, Institute of Bioorganic Chemistry, Poznań, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Rodziewicz
authors.affiliation: Polish Academy of Science, Institute of Bioorganic Chemistry, Poznań, Poland.
authors.forename: Klaudia
authors.initials: K
authors.lastname: Chmielewska
authors.affiliation: Silesian University of Technology, Gliwice, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Polański
authors.affiliation: Silesian University of Technology, Gliwice, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Polańska
authors.affiliation: Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gdowicz-Kłosok
authors.affiliation: Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Giglok
authors.affiliation: Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Suwiński
authors.affiliation: Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Tarnawski
authors.affiliation: Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Dziadziuszko
authors.affiliation: Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Witold
authors.initials: W
authors.lastname: Rzyman
authors.affiliation: Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Widłak
mesh_terms: D000230:Adenocarcinoma / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016632:Apolipoprotein A-I / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D016022:Case-Control Studies; D018450:Disease Progression; D042241:Early Diagnosis; D005260:Female; D006242:Haptoglobins / Q000032:analysis; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020543:Proteome / Q000032:analysis; D000685:Serum Amyloid A Protein / Q000032:analysis; D053482:beta 2-Glycoprotein I / Q000097:blood
chemical_list: D016632:Apolipoprotein A-I; D014408:Biomarkers, Tumor; D001798:Blood Proteins; C485359:HP protein, human; D006242:Haptoglobins; D020543:Proteome; D000685:Serum Amyloid A Protein; D053482:beta 2-Glycoprotein I