title: Iminodiacetic acid functionalized porous hydroxyapatite nanoparticles for capturing histidine-tagged proteins.
abstract: A simple strategy has been developed to synthesize hydroxyapatite (HAP) nanoparticles (NPs) in a simulated body fluid (SBF). The HAP NPs have an average diameter of 50nm and present porous structure. By taking advantage of surface hydroxyl groups, the HAP NPs are further modified with iminodiacetic acid (IDA), followed by chelating Ni(2+) ions. The HAP/IDA-Ni(2+) NPs as novel adsorbent can capture directly histidine-tagged (His-tagged) proteins from the mixture of lysed cells without sample pretreatment. Results indicated that the HAP/IDA-Ni(2+) NPs present negligible nonspecific adsorption and high protein binding ability, and their specificity and affinity toward His-tagged proteins can remain after 5 times of recycling. The HAP/IDA-Ni(2+) NPs are especially suitable for purification of His-tagged proteins with low molecule weight.
authors.affiliation: Key Laboratory for Special Functional Materials, Henan University, Kaifeng 475004, PR China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Yao
authors.affiliation: Key Laboratory of Plant Stress Biology, Henan University, Kaifeng 475004, PR China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory for Special Functional Materials, Henan University, Kaifeng 475004, PR China. Electronic address: zhaoyb902@henu.edu.cn.
authors.forename: Yanbao
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Plant Stress Biology, Henan University, Kaifeng 475004, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Special Functional Materials, Henan University, Kaifeng 475004, PR China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zou
authors.affiliation: Key Laboratory of Plant Stress Biology, Henan University, Kaifeng 475004, PR China.
authors.forename: Chunpeng
authors.initials: C
authors.lastname: Song
mesh_terms: D000327:Adsorption; D002614:Chelating Agents / Q000737:chemistry; D017886:Durapatite / Q000138:chemical synthesis*; D004926:Escherichia coli / Q000378:metabolism; D006639:Histidine / Q000737:chemistry*; D007098:Imino Acids / Q000138:chemical synthesis*; D007477:Ions / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D016062:Porosity; D011485:Protein Binding; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D002614:Chelating Agents; D007098:Imino Acids; D007477:Ions; D006639:Histidine; D009532:Nickel; D017886:Durapatite; C008109:iminodiacetic acid
keywords: His-tagged protein; Hydroxyapatite; Preparation; Separation