title: Differential expression of molybdenum transport and assimilation genes between two winter wheat cultivars (Triticum aestivum).
abstract: Molybdenum (Mo) is an essential trace element for higher plants. Winter wheat cultivar 97003 has a higher Mo efficiency than 97014 under Mo-deficiency stress. Mo efficiency is related to Mo uptake, transfer and assimilation in plants. Several genes are involved in regulating Mo uptake, transfer and assimilation in plants. To obtain a better understanding of the aforementioned difference in Mo uptake, we have conducted a hydroponic trail to investigate the expression of genes related to Mo uptake, transfer and assimilation in the above two cultivars. The results indicate a closed relationship between Mo uptake and TaSultr5.1, TaSultr5.2 and TaCnx1 expression, according to a stepwise regression analysis of the time course of Mo uptake in the two cultivars. Meanwhile, expression of TaSultr5.2 in roots also showed a positive relationship with Mo uptake rates. 97003 had stronger Mo uptake than 97014 at low Mo-application rates (less than 1 μmol Mo L(-1)) due to the higher expression of TaSultr5.2, TaSultr5.1 and TaCnx1 in roots. On the contrary, Mo uptake of 97003 was weaker than 97014 at high Mo application rates (ranging from 5 to 20 μmol Mo L(-1)), which was related to significant down-regulation of TaSultr5.2 and TaCnx1 genes in roots of 97003 compared to 97014. Therefore, we speculated that the differential-expression intensities of TaSultr5.2, TaSultr5.1 and TaCnx1 could be the cause of the difference in Mo uptake between the two winter wheat cultivars at low and high Mo application levels.
authors.affiliation: Micro-element Research Center, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Nie
authors.affiliation: Micro-element Research Center, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chengxiao
authors.initials: C
authors.lastname: Hu
authors.affiliation: Micro-element Research Center, Huazhong Agricultural University, Wuhan 430070, China; College of Resources and Environment, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Hongen
authors.initials: H
authors.lastname: Liu
authors.affiliation: Micro-element Research Center, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Qiling
authors.initials: Q
authors.lastname: Tan
authors.affiliation: Micro-element Research Center, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: sxccn@mail.hzau.edu.cn.
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Sun
mesh_terms: D018506:Gene Expression Regulation, Plant; D008982:Molybdenum / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D008982:Molybdenum
keywords: Assimilation; Gene expression; Molybdenum; Transport; Winter wheat