title: Microarray analysis of serum mRNA in patients with head and neck squamous cell carcinoma at whole-genome scale.
abstract: With the increasing demand for noninvasive approaches in monitoring head and neck cancer, circulating nucleic acids have been shown to be a promising tool. We focused on the global transcriptome of serum samples of head and neck squamous cell carcinoma (HNSCC) patients in comparison with healthy individuals. We compared gene expression patterns of 36 samples. Twenty-four participants including 16 HNSCC patients (from 12 patients we obtained blood samples 1 year posttreatment) and 8 control subjects were recruited. The Illumina HumanWG-6 v3 Expression BeadChip was used to profile and identify the differences in serum mRNA transcriptomes. We found 159 genes to be significantly changed (Storey's P value <0.05) between normal and cancer serum specimens regardless of factors including p53 and B-cell lymphoma family members (Bcl-2, Bcl-XL). In contrast, there was no difference in gene expression between samples obtained before and after surgery in cancer patients. We suggest that microarray analysis of serum cRNA in patients with HNSCC should be suitable for refinement of early stage diagnosis of disease that can be important for development of new personalized strategies in diagnosis and treatment of tumours but is not suitable for monitoring further development of disease.
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Charles University, University Hospital Motol, Institute of Postgraduate Medicine, V Uvalu 84, 15006 Prague 5, Czech Republic ; Academy of Sciences of the Czech Republic, Institute of Molecular Genetics, Department of Genomics and Bioinformatics, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Markéta
authors.initials: M
authors.lastname: Čapková
authors.affiliation: Academy of Sciences of the Czech Republic, Institute of Molecular Genetics, Department of Genomics and Bioinformatics, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Šáchová
authors.affiliation: Academy of Sciences of the Czech Republic, Institute of Molecular Genetics, Department of Genomics and Bioinformatics, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Hynek
authors.initials: H
authors.lastname: Strnad
authors.affiliation: Academy of Sciences of the Czech Republic, Institute of Molecular Genetics, Department of Genomics and Bioinformatics, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Michal
authors.identifier: 0000-0001-5335-5444
authors.initials: M
authors.lastname: Kolář
authors.affiliation: Academy of Sciences of the Czech Republic, Institute of Molecular Genetics, Department of Genomics and Bioinformatics, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Miluše
authors.initials: M
authors.lastname: Hroudová
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Charles University, University Hospital Motol, Institute of Postgraduate Medicine, V Uvalu 84, 15006 Prague 5, Czech Republic ; Institute of Anatomy, 1st Faculty of Medicine, Charles University, U Nemocnice 3, 12800 Prague 2, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Chovanec
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Charles University, University Hospital Motol, Institute of Postgraduate Medicine, V Uvalu 84, 15006 Prague 5, Czech Republic.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Čada
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Charles University, University Hospital Motol, Institute of Postgraduate Medicine, V Uvalu 84, 15006 Prague 5, Czech Republic ; Academy of Sciences of the Czech Republic, Institute of Molecular Genetics, Department of Genomics and Bioinformatics, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Šteffl
authors.affiliation: Department of Stomatology, 1st Faculty of Medicine, Charles University, Kateřinská 32, 12108 Prague 2, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Valach
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Charles University, University Hospital Motol, Institute of Postgraduate Medicine, V Uvalu 84, 15006 Prague 5, Czech Republic.
authors.forename: Jan
authors.identifier: 0000-0003-3389-658X
authors.initials: J
authors.lastname: Kastner
authors.affiliation: Academy of Sciences of the Czech Republic, Institute of Molecular Genetics, Department of Genomics and Bioinformatics, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Čestmír
authors.initials: Č
authors.lastname: Vlček
authors.affiliation: Institute of Anatomy, 1st Faculty of Medicine, Charles University, U Nemocnice 3, 12800 Prague 2, Czech Republic.
authors.forename: Karel
authors.identifier: 0000-0002-7200-9755
authors.initials: K
authors.lastname: Smetana
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Charles University, University Hospital Motol, Institute of Postgraduate Medicine, V Uvalu 84, 15006 Prague 5, Czech Republic.
authors.forename: Jan
authors.identifier: 0000-0003-3710-4455
authors.initials: J
authors.lastname: Plzák
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D003710:Demography; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D025341:Principal Component Analysis; D012333:RNA, Messenger / Q000097:blood* / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D000077195:Squamous Cell Carcinoma of Head and Neck; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53