title: Mimicking the 2-oxoglutaric acid signalling function using molecular probes: insights from structural and functional investigations.
abstract: 2-Oxoglutaric acid (2-OG) has gained considerable attention because of its newly discovered signalling role in addition to its established metabolic functions. With the aim of further exploring the signalling function of 2-OG, here we present a structure-activity relationship study using 2-OG probes bearing different carbon chain lengths and terminal groups. Our results highlight the importance of the five-membered carbon molecular skeleton and of the two carboxylic terminals in maintaining the signalling functions of the parent molecule 2-OG. These findings provide valuable information for designing new, effective molecular probes able to dissect and discriminate the newly discovered, complex signalling role of 2-OG from its canonical activity in metabolism.
authors.affiliation: Aix-Marseille Université, CNRS, CINaM UMR 7325, 13288, Marseille, France. ling.peng@univ-amu.fr.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xinjun
authors.initials: X
authors.lastname: Liu
authors.forename: Erik
authors.initials: E
authors.lastname: Laurini
authors.forename: Paola
authors.initials: P
authors.lastname: Posocco
authors.forename: Fabio
authors.initials: F
authors.lastname: Ziarelli
authors.forename: Maurizio
authors.initials: M
authors.lastname: Fermeglia
authors.forename: Fanqi
authors.initials: F
authors.lastname: Qu
authors.forename: Sabrina
authors.initials: S
authors.lastname: Pricl
authors.forename: Cheng-Cai
authors.initials: CC
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Peng
mesh_terms: D017033:Anabaena / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D066244:Proton Magnetic Resonance Spectroscopy; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D007656:Ketoglutaric Acids; D015335:Molecular Probes; D014157:Transcription Factors; D004247:DNA