title: PCR amplification of SNP loci from crude DNA for large-scale genotyping of oomycetes.
abstract: Similar to other eukaryotes, single nucleotide polymorphism (SNP) markers are abundant in many oomycete plant pathogen genomes. High resolution DNA melting analysis (HR-DMA) is a cost-effective method for SNP genotyping, but like many SNP marker technologies, is limited by the amount and quality of template DNA. We describe PCR preamplification of Phytophthora and Peronospora SNP loci from crude DNA extracted from a small amount of mycelium and/or infected plant tissue to produce sufficient template to genotype at least 10 000 SNPs. The approach is fast, inexpensive, requires minimal biological material and should be useful for many organisms in a variety of contexts.
authors.affiliation: Department of Plant Pathology, China Agricultural University, Beijing, and Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, Tennessee 37996.
authors.forename: Jian
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, Tennessee 37996.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Lyon
authors.affiliation: Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, Tennessee 37996.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, Tennessee 37996 klamour@utk.edu.
authors.forename: Kurt
authors.initials: K
authors.lastname: Lamour
mesh_terms: D004247:DNA / Q000235:genetics*; D005838:Genotype; D009868:Oomycetes / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D004247:DNA
keywords: Peronospora; Phytophthora; SNP genotyping