title: The zoonotic risk of Ancylostoma ceylanicum isolated from stray dogs and cats in Guangzhou, South China.
abstract: Canine and feline hookworm infection is endemic in many countries with zoonotic transmission representing a potentially significant public health concern. However, there is limited data available on the zoonotic transmission of canine and feline hookworms in China. This study was conducted to evaluate the zoonotic risk of Ancylostoma ceylanicum isolated from stray dogs and cats in Guangzhou, south China. Primer pairs CAF/CAR were designed to amplify complete ITS sequences of obtained A. ceylanicum. The results were compared with fourteen ITS reference sequences of human-derived A. ceylanicum registered in GenBank, and phylogenetic trees were established by using NJ and ML methods. The sequence similarity of three dog-derived and five cat-derived A. ceylanicum with fourteen human-derived A. ceylanicum were 96.8%~100% and 97.8%~100%, respectively. Phylogenetic analysis placed A. ceylanicum isolated from dogs and cats in the same group with A. ceylanicum human isolates. Due to the ability of A. ceylanicum to cause a patent infection in humans, the zoonotic risk arising from dog and cat reservoirs to communities in this region should be determined.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yuanjia
authors.identifier: 0000-0002-0591-0797
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Guochao
authors.initials: G
authors.lastname: Zheng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Muhamd
authors.identifier: 0000-0002-1050-5454
authors.initials: M
authors.lastname: Alsarakibi
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xinheng
authors.identifier: 0000-0002-3061-3743
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Tan
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Luo
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Pengyun
authors.initials: P
authors.lastname: Lu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Li
mesh_terms: D000722:Ancylostoma / Q000235:genetics* / Q000302:isolation & purification*; D000724:Ancylostomiasis / Q000453:epidemiology / Q000235:genetics* / Q000635:transmission; D000818:Animals; D002415:Cats; D002681:China / Q000453:epidemiology; D018509:DNA, Helminth / Q000235:genetics*; D004285:Dogs; D005260:Female; D006801:Humans; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012307:Risk Factors; D015047:Zoonoses / Q000453:epidemiology / Q000235:genetics* / Q000635:transmission
chemical_list: D018509:DNA, Helminth