title: Three major nucleolar proteins migrate from nucleolus to nucleoplasm and cytoplasm in root tip cells of Vicia faba L. exposed to aluminum.
abstract: Results from our previous investigation indicated that Al could affect the nucleolus and induce extrusion of silver-staining nucleolar particles containing argyrophilic proteins from the nucleolus into the cytoplasm in root tip cells of Vicia faba L. So far, the nucleolar proteins involved have not been identified. It is well known that nucleophosmin (B23), nucleolin (C23), and fibrillarin are three major and multifunctional nucleolar proteins. Therefore, effects of Al on B23, C23, and fibrillarin in root tip cells of V. faba exposed to 100 μM Al for 48 h were observed and analyzed using indirect immunofluorescence microscopy and Western blotting. The results from this work demonstrated that after 100 μM of Al treatment for 48 h, B23 and C23 migrated from the nucleolus to the cytoplasm and fibrillarin from the nucleolus to the nucleoplasm. In some cells, fibrillarin was present only in the cytoplasm. Western blotting data revealed higher expression of the three major nucleolar proteins in Al-treated roots compared with the control and that the B23 content increased markedly. These findings confirmed our previous observations.
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Qin
authors.forename: Huaning
authors.initials: H
authors.lastname: Zhang
authors.forename: Shaoshan
authors.initials: S
authors.lastname: Li
authors.forename: Wusheng
authors.initials: W
authors.lastname: Jiang
authors.forename: Donghua
authors.initials: D
authors.lastname: Liu
mesh_terms: D000535:Aluminum / Q000378:metabolism*; D002466:Cell Nucleolus / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D018519:Meristem / Q000166:cytology / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D021381:Protein Transport; D016601:RNA-Binding Proteins / Q000378:metabolism*; D031307:Vicia faba / Q000166:cytology / Q000378:metabolism*; D000097796:Nucleolin
chemical_list: D000535:Aluminum; D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D000090243:Nucleophosmin; D010750:Phosphoproteins; D010940:Plant Proteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin; C047458:fibrillarin