title: High antibody titres against predicted Mycoplasma surface proteins do not prevent sequestration in infected lung tissue in the course of experimental contagious bovine pleuropneumonia.
abstract: Contagious bovine pleuropneumonia (CBPP), a severe respiratory disease of cattle caused by Mycoplasma mycoides subsp. mycoides (Mmm) is endemic in many African countries due to fragmented veterinary services and the lack of an efficient vaccine and sensitive diagnostics. More efficient tools to control the disease are needed, but to develop the tools, a better understanding of host-pathogen interactions is necessary. The aim of this study was to characterize the kinetics of the humoral immune response against 65 Mmm surface antigens for an extended period in cattle that survived a primary infection with Mmm. We describe clinical and haematological outcomes, and dissect the humoral immune response over time, to specific antigens and compared the antibody responses between different pathomorphological outcomes. No antigen-specific antibodies correlating with protection were identified. Interestingly we found that animals that developed Mycoplasma-containing sequestra had significantly higher antibody levels against proteins comprising the surface proteome than the animals that cleared Mycoplasma from their lungs. Based on these data we suggest that high antibody titres might play a role in the establishment of pathomorphological changes, such as vasculitis, which should be investigated in future studies. Beneficial antibody specificities and cellular immune responses need to be identified in order to foster the development of an improved vaccine in the future.
authors.affiliation: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100 Nairobi, Kenya.
authors.forename: Elise
authors.initials: E
authors.lastname: Schieck
authors.affiliation: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100 Nairobi, Kenya.
authors.forename: Anne
authors.initials: A
authors.lastname: Liljander
authors.affiliation: Department of Proteomics, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Center, SE-106 91 Stockholm, Sweden.
authors.forename: Carl
authors.initials: C
authors.lastname: Hamsten
authors.affiliation: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100 Nairobi, Kenya.
authors.forename: Nimmo
authors.initials: N
authors.lastname: Gicheru
authors.affiliation: Istituto Zooprofilattico Sperimentale of Abruzzo and Molise "G. Caporale", Via Campo Boario, 64100 Teramo, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Scacchia
authors.affiliation: Istituto Zooprofilattico Sperimentale of Abruzzo and Molise "G. Caporale", Via Campo Boario, 64100 Teramo, Italy.
authors.forename: Flavio
authors.initials: F
authors.lastname: Sacchini
authors.affiliation: Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Naumburger Strasse 96a, 07743 Jena, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Heller
authors.affiliation: Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Naumburger Strasse 96a, 07743 Jena, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Schnee
authors.affiliation: Center for Experimental Medicine, University of Cologne, Medical School, University Hospital of Cologne, Robert-Kochstr. 10, 50924 Cologne, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Sterner-Kock
authors.affiliation: Landeslabor Berlin Brandenburg, Gerhard-Neumann-Str. 2-3, 15236 Frankfurt (Oder), Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hlinak
authors.affiliation: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100 Nairobi, Kenya.
authors.forename: Jan
authors.initials: J
authors.lastname: Naessens
authors.affiliation: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100 Nairobi, Kenya.
authors.forename: Jane
authors.initials: J
authors.lastname: Poole
authors.affiliation: Department of Proteomics, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Center, SE-106 91 Stockholm, Sweden.
authors.forename: Anja
authors.initials: A
authors.lastname: Persson
authors.affiliation: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100 Nairobi, Kenya. Electronic address: jores@web.de.
authors.forename: Joerg
authors.initials: J
authors.lastname: Jores
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D056724:Immunity, Humoral; D007630:Kenya; D008168:Lung / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology; D009176:Mycoplasma mycoides / Q000235:genetics / Q000276:immunology*; D011002:Pleuropneumonia, Contagious / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D020543:Proteome / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008565:Membrane Proteins; D020543:Proteome
keywords: Antibody response; Contagious bovine pleuropneumonia (CBPP); Mycoplasma mycoides subsp. mycoides; Oxytetracycline; Sequestra; White blood count