title: New coumarins and anti-inflammatory constituents from the fruits of Cnidium monnieri.
abstract: The fruit of Cnidium monnieri is commercially used as healthcare products for the improvement of impotence and skin diseases. Three new coumarins, 3'-O-methylmurraol (1), rel-(1'S,2'S)-1'-O-methylphlojodicarpin (2), and (1'S,2'S)-1'-O-methylvaginol (3), have been isolated from the fruits of C. monnieri, together with 14 known compounds (4-17). The structures of these new compounds were determined through spectroscopic and MS analyses. Compounds 1, 4-12, and 14-17 exhibited inhibition (IC50 ≤ 7.31 µg/mL) of superoxide anion generation by human neutrophils in response to formyl-l-methionyl-l-leucyl-l-phenylalanine/cytochalasin B (fMLP/CB). Compounds 7, 9-11, 15, and 17 inhibited fMLP/CB-induced elastase release with IC50 values ≤7.83 µg/mL. This investigation reveals that bioactive isolates (especially 6, 7, 14, and 17) could be further developed as potential candidates for the treatment or prevention of various inflammatory diseases.
authors.affiliation: Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 110, Taiwan. thlee@tmu.edu.tw.
authors.forename: Tzong-Huei
authors.initials: TH
authors.lastname: Lee
authors.affiliation: Department of Pharmacy & Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung 907, Taiwan. cute88883333@yahoo.com.tw.
authors.forename: Yuan-Chih
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan. htl@mail.cgu.edu.tw.
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.affiliation: Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan. cwshu@vghks.gov.tw.
authors.forename: Chih-Wen
authors.initials: CW
authors.lastname: Shu
authors.affiliation: National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. pjsung@nmmba.gov.tw.
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
authors.affiliation: School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan. limyp@mail2000.com.tw.
authors.forename: Yun-Ping
authors.initials: YP
authors.lastname: Lim
authors.affiliation: Chung-Jen College of Nursing, Health Science and Management, Chiayi 600, Taiwan. m049@cjc.edu.tw.
authors.forename: Wen-Lung
authors.initials: WL
authors.lastname: Kuo
authors.affiliation: Department of Pharmacy & Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung 907, Taiwan. jjchen@tajen.edu.tw.
authors.forename: Jih-Jung
authors.initials: JJ
authors.lastname: Chen
mesh_terms: D000328:Adult; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D031048:Cnidium / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D010196:Pancreatic Elastase / Q000276:immunology; D013481:Superoxides / Q000276:immunology; D055815:Young Adult
chemical_list: D000893:Anti-Inflammatory Agents; D003374:Coumarins; D013481:Superoxides; D010196:Pancreatic Elastase