title: 3D-printed and CNC milled flow-cells for chemiluminescence detection.
abstract: Herein we explore modern fabrication techniques for the development of chemiluminescence detection flow-cells with features not attainable using the traditional coiled tubing approach. This includes the first 3D-printed chemiluminescence flow-cells, and a milled flow-cell designed to split the analyte stream into two separate detection zones within the same polymer chip. The flow-cells are compared to conventional detection systems using flow injection analysis (FIA) and high performance liquid chromatography (HPLC), with the fast chemiluminescence reactions of an acidic potassium permanganate reagent with morphine and a series of adrenergic phenolic amines.
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3216, Australia.
authors.forename: Kara B
authors.initials: KB
authors.lastname: Spilstead
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3216, Australia.
authors.forename: Jessica J
authors.initials: JJ
authors.lastname: Learey
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3216, Australia. Electronic address: egan.doeven@deakin.edu.au.
authors.forename: Egan H
authors.initials: EH
authors.lastname: Doeven
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3216, Australia.
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Barbante
authors.affiliation: School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M60 1QD, UK.
authors.forename: Stephan
authors.initials: S
authors.lastname: Mohr
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3216, Australia.
authors.forename: Neil W
authors.initials: NW
authors.lastname: Barnett
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3216, Australia.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Terry
authors.affiliation: School of Engineering, Deakin University, Geelong, Victoria 3216, Australia.
authors.forename: Robynne M
authors.initials: RM
authors.lastname: Hall
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3216, Australia. Electronic address: paul.francis@deakin.edu.au.
authors.forename: Paul S
authors.initials: PS
authors.lastname: Francis
mesh_terms: D000143:Acids / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry; D000588:Amines / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D017022:Flow Injection Analysis / Q000379:methods; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods*; D009020:Morphine / Q000032:analysis / Q000737:chemistry; D009655:Octopamine / Q000032:analysis / Q000737:chemistry; D019800:Phenol / Q000032:analysis* / Q000737:chemistry; D011196:Potassium Permanganate / Q000737:chemistry; D066330:Printing, Three-Dimensional; D015203:Reproducibility of Results; D013578:Synephrine / Q000032:analysis / Q000737:chemistry; D014439:Tyramine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry
chemical_list: D000143:Acids; D000180:Acrylic Resins; D000588:Amines; D011196:Potassium Permanganate; D009655:Octopamine; D019800:Phenol; D009020:Morphine; C007964:hordenine; D013578:Synephrine; D014439:Tyramine
keywords: 3D-printing; Acidic potassium permanganate; CNC milling; Chemiluminescence; Flow injection analysis; Flow-cells