title: Coeliac disease--clinical presentation and diagnosis by anti tissue transglutaminase antibodies titre in children.
abstract: OBJECTIVE
  To study the spectrum of clinical presentation of coeliac disease and the role of IgA anti-tissue transglutaminase antibodies titer in the diagnosis and effect of gluten-free diet on such titers in children.
  
  
  METHODS
  The prospective study was conducted in the paediatric department of Combined Military Hospital, Kharian from Sep 2011 to Sep 2012. Children of 1-12 years of age presenting with chronic diarrhoea, malnutrition and failure to thrive were included regardless of gender, socioeconomic status, ethnicity and geographical distribution. Anti-tissue transglutaminase angibodies titers were done on enrolment. Patients with levels more than 30 u/ml were enrolled. They were advised strict gluten-free diet for six months. These titers were repeated after six months to document the effect of gluten-free diet on these titers. Paediatric endoscopy and duodenal biopsy facilities were not available at the study site, so the response was monitored through titers. Data was analysed using SPSS-20.
  
  
  RESULTS
  Out of 61 patients with IgA levels more than 10 u/ml, 52 (85.24%) were found to have a positive (> 30u/ml) anti-tissue transglutaminase antibodies titers with a mean value of 42.67 +/- 7.60 U/ml. These 52 patients were then put on a trial of gluten-free diet for six months after which significant reduction in titer was noticed, with a mean value of 13.25 +/- 2.59 U/ml. This reduction in titer was associated with marked clinical improvement and regression of symptoms. Frequency of different clinical features in descending order revealed that chronic diarrhoea, abdominal distension, iron deficiency anaemia, failure to thrive, pallor and rickets were present in 38 (73.1%), 30 (57.7%), 29 (55.8%), 29 (53.8%), 28 (53.8%) patients respectively.
  
  
  CONCLUSIONS
  Chronic diarrhoea, failure to thrive, pallor, abdominal distention and iron deficiency anaemia were common modes of presentation. The antibodies were strongly positive in most of the cases. All children showed significant improvement in clinical features and reduction in antibody titers after six-month trial of gluten free diet.
authors.forename: Shabbir
authors.initials: S
authors.lastname: Hussain
authors.forename: Moin-ud-Din
authors.initials: MU
authors.lastname: Sabir
authors.forename: Mohammad
authors.initials: M
authors.lastname: Afzal
authors.forename: Imran
authors.initials: I
authors.lastname: Asghar
mesh_terms: D000906:Antibodies / Q000032:analysis; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D002446:Celiac Disease / Q000175:diagnosis* / Q000276:immunology; D002675:Child, Preschool; D005260:Female; D019204:GTP-Binding Proteins / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D007223:Infant; D008297:Male; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases / Q000276:immunology*
chemical_list: D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins