title: Mitochondrial protein targets of radiosensitisation by 1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone on nasopharyngeal carcinoma cells.
abstract: In our preliminary study, 1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone (GXHSWAQ-1), synthesised according to the basic structure of emodin, exhibited a 1.58-fold radiosensitisation on nasopharyngeal carcinoma CNE-1 cells. This study demonstrated that its radiosensitisation activity was achieved by altering the mitochondrial structure: swollen volume, fragmented crista, and decreasing transmembrane potential (P<0.01). Using isobaric tag for relative and absolute quantitation (iTRAQ) technology, 1396 proteins were identified, and the differentially expressed proteins were involved in metabolism, cell proliferation, angiogenesis, DNA repair process according to the biological process clustering results. Bioinformatic analysis showed that CDH1, RAC1, CDC42 proteins might be mostly mitochondrial targets in the radiosensitisation process. Western blotting analyses verified the differential expression of these proteins.
authors.affiliation: College of Pharmacy, Guangxi Medical University, Nanning 530021 China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Mo
authors.affiliation: College of Pharmacy, Guangxi Medical University, Nanning 530021 China. Electronic address: houhuaxin@163.com.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Hou
authors.affiliation: Guangxi Institute for Cancer Research, Nanning 530021, China. Electronic address: danrongli@163.com.
authors.forename: Danrong
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Pharmacy, Guangxi Medical University, Nanning 530021 China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: College of Pharmacy, Guangxi Medical University, Nanning 530021 China.
authors.forename: Donglian
authors.initials: D
authors.lastname: Chen
authors.affiliation: Guangxi Institute for Cancer Research, Nanning 530021, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000880:Anthraquinones / Q000494:pharmacology*; D002277:Carcinoma; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000528:radiation effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D024101:Mitochondrial Proteins / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D040901:Proteomics; D011836:Radiation Tolerance / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects
chemical_list: C000595056:3-acetyl-1,8-dihydroxy-6-methyl-9,10-anthraquinone; D000880:Anthraquinones; D024101:Mitochondrial Proteins
keywords: 1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone; Bioinformatics; Mitochondrial proteins; Nasopharyngeal carcinoma (NPC); Radiosensitisation; iTRAQ