title: Houttuynia cordata attenuates lipid accumulation via activation of AMP-activated protein kinase signaling pathway in HepG2 cells.
abstract: Houttuynia cordata (H. cordata) from the family Saururaceae is a perennial herb native to Southeast Asia. It possesses a range of medicinal properties to treat several disease symptoms including allergic inflammation and anaphylaxis. In the present investigation, we provided the molecular mechanisms underlying the role of H. cordata extract (HCE) in the prevention of high glucose-induced lipid accumulation in human HepG2 hepatocytes. HepG2 cells were pre-treated with various concentrations of HCE (0, 10, 20, 40, and 80 μg/mL) and treated with serum-free medium with normal glucose (5 mM) for 1 h, followed by exposure to high glucose (25 mM D-glucose) for 24 h. HCE significantly and dose-dependently attenuated lipid accumulation in human HepG2 hepatocytes when exposed to high glucose (25 mM D-glucose) (p < 0.05, p < 0.01 and p < 0.001 at 20, 40, and 80 μg/mL concentrations, respectively). Further, HCE attenuated the expression of fatty acid synthase (FAS), sterol regulatory element-binding protein-1 and glycerol 3-phosphate acyltransferases (GPATs). The adenosine monophosphate-activated protein kinase (AMPK) was also activated by HCE treatment when exposed to high glucose (25 mM D-glucose) in human HepG2 hepatocytes. This study suggests the hypolipidemic effects of HCE by the inhibition of lipid biosynthesis mediated through AMPK signaling, which may play an active role and can be developed as an anti-obesity agent.
authors.affiliation: Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan, 330-714, Republic of Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Kang
authors.forename: Sushruta
authors.initials: S
authors.lastname: Koppula
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism / Q000502:physiology*; D019440:Anti-Obesity Agents; D004305:Dose-Response Relationship, Drug; D055808:Drug Discovery; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D064429:Fatty Acid Synthases / Q000378:metabolism; D005947:Glucose / Q000009:adverse effects; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism; D056945:Hep G2 Cells; D052859:Houttuynia / Q000737:chemistry*; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism
chemical_list: D019440:Anti-Obesity Agents; D004365:Drugs, Chinese Herbal; C442781:Houttuynia cordata extract; D051780:Sterol Regulatory Element Binding Protein 1; D005992:Glycerol-3-Phosphate O-Acyltransferase; D064429:Fatty Acid Synthases; D055372:AMP-Activated Protein Kinases; D005947:Glucose