title: Marker-assisted selection for recognizing wheat mutant genotypes carrying HMW glutenin alleles related to baking quality.
abstract: Allelic diversity of HMW glutenin loci in several studies revealed that allelic combinations affect dough quality. Dx5 + Dy10 subunits are related to good baking quality and Dx2 + Dy12 are related to undesirable baking quality. One of the most regular methods to evaluate the baking quality is SDS-PAGE which is used to improve baking quality labs. Marker-assisted selection is the method which can recognize the alleles related to baking quality and this method is based on polymerase chain reaction. 10 pairs of specific primers related to Dx2, Dx2.1, Dx5, Dy10, and Dy12 subunits were used for recognizing baking quality of some wheat varieties and some mutant genotypes. Only 5 pairs of them could show the specific bands. All subunits were recognized by the primers except Dx2.1. Some of the primers were extracted from previous studies and the others were designed based on D genome subunits of wheat. SDS-PAGE method accomplished having confidence in these marker's results. To realize the effect of mutation, seed storage proteins were measured. It showed that mutation had effect on the amount of seed storage protein on the mutant seeds (which showed polymorphism).
authors.affiliation: Seed and Plant Improvement Institute, Fahmideh Boulevard, Karaj, Iran.
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Zamani
authors.affiliation: Agriculture College, Tehran University, Karaj, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Bihamta
authors.affiliation: Agricultural, Medical and Industrial Research School, Karaj, Iran.
authors.forename: Behnam
authors.initials: B
authors.lastname: Naserian Khiabani
authors.affiliation: Agriculture College, Zanjan University, Zanjan, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Tahernezhad
authors.affiliation: Agricultural, Medical and Industrial Research School, Karaj, Iran.
authors.forename: Mohammad Taher
authors.initials: MT
authors.lastname: Hallajian
authors.affiliation: Agriculture College, University of Payam Nour, Kaboudarahang Branch, Kaboudarahang, Iran.
authors.forename: Marzieh Varasteh
authors.initials: MV
authors.lastname: Shamsi
mesh_terms: D000483:Alleles; D001939:Bread / Q000592:standards*; D003296:Cooking; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D005983:Glutens / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D014908:Triticum / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D005983:Glutens; C037302:glutenin