title: Therapeutic effect of bone marrow mesenchymal stem cells on laser-induced retinal injury in mice.
abstract: Stem cell therapy has shown encouraging results for neurodegenerative diseases. The retina provides a convenient locus to investigate stem cell functions and distribution in the nervous system. In the current study, we investigated the therapeutic potential of bone marrow mesenchymal stem cells (MSCs) by systemic transplantation in a laser-induced retinal injury model. MSCs from C57BL/6 mice labeled with green fluorescent protein (GFP) were injected via the tail vein into mice after laser photocoagulation. We found that the average diameters of laser spots and retinal cell apoptosis were decreased in the MSC-treated group. Interestingly, GFP-MSCs did not migrate to the injured retina. Further examination revealed that the mRNA expression levels of glial fibrillary acidic protein and matrix metalloproteinase-2 were lower in the injured eyes after MSC transplantation. Our results suggest that intravenously injected MSCs have the ability to inhibit retinal cell apoptosis, reduce the inflammatory response and limit the spreading of damage in the laser-injured retina of mice. Systemic MSC therapy might play a role in neuroprotection, mainly by regulation of the intraocular microenvironment.
authors.affiliation: Tianjin Medical University Eye Hospital and Eye Institute, No. 251, Fukang Road, Nankai District, Tianjin 300384, China. frankjyf@126.com.
authors.forename: Yuanfeng
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Tianjin Medical University Eye Hospital and Eye Institute, No. 251, Fukang Road, Nankai District, Tianjin 300384, China. yanzhang9927@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Medical University Eye Hospital and Eye Institute, No. 251, Fukang Road, Nankai District, Tianjin 300384, China. zhanglingjun08@163.com.
authors.forename: Lingjun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, Haibin People's Hospital of Tianjin, No. 400, Chuangye Road, Binhai New District, Tianjin 300280, China. tongxin1506@sina.com.
authors.forename: Meiyan
authors.initials: M
authors.lastname: Wang
authors.affiliation: Tianjin Medical University Eye Hospital and Eye Institute, No. 251, Fukang Road, Nankai District, Tianjin 300384, China. xiaomzh@126.com.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Tianjin Medical University Eye Hospital and Eye Institute, No. 251, Fukang Road, Nankai District, Tianjin 300384, China. xiaorli@163.com.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D002465:Cell Movement; D005260:Female; D005786:Gene Expression Regulation; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D007834:Lasers / Q000009:adverse effects*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics; D012160:Retina / Q000166:cytology / Q000293:injuries* / Q000378:metabolism / Q000473:pathology*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D020778:Matrix Metalloproteinase 2