title: Self-assembly of phosphate amphiphiles in mixtures of prebiotically plausible surfactants.
abstract: The spontaneous formation of closed bilayer structures from prebiotically plausible amphiphiles is an essential requirement for the emergence of early cells on prebiotic Earth. The sources of amphiphiles could have been both endo- and exogenous (accretion of meteorite carbonaceous material or interstellar dust particles). Among all prebiotic possible amphiphile candidates, those containing phosphate are the least investigated species because their self-assembly occurs in a seemingly too narrow range of conditions. The self-assembly of simple phosphate amphiphiles should, however, be of great interest, as contemporary membranes predominantly contain phospholipids. In contrast to common expectations, we show that these amphiphiles can be easily synthesized under prebiotically plausible environmental conditions and can efficiently form bilayer structures in the presence of various co-surfactants across a large range of pH values. Vesiculation was even observed in crude reaction mixtures that contained 1-decanol as the amphiphile precursor. The two best co-surfactants promoted vesicle formation over the entire pH range in aqueous solutions. Expanding the pH range where bilayer membranes self-assemble and remain intact is a prerequisite for the emergence of early cell-like compartments and their preservation under fluctuating environmental conditions. These mixed bilayers also retained small charged solutes, such as dyes. These results demonstrate that alkyl phosphate amphiphiles might have played a significant role as early compartment building blocks.
authors.affiliation: 1 Department of Physics, Chemistry and Pharmacy, University of Southern Denmark , Odense, Denmark .
authors.forename: A N
authors.initials: AN
authors.lastname: Albertsen
authors.forename: C D
authors.initials: CD
authors.lastname: Duffy
authors.forename: J D
authors.initials: JD
authors.lastname: Sutherland
authors.forename: P-A
authors.initials: PA
authors.lastname: Monnard
mesh_terms: D005452:Fluoresceins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010755:Organophosphates / Q000737:chemistry; D010539:Permeability; D010710:Phosphates / Q000737:chemistry*; D038761:Photobleaching; D013501:Surface-Active Agents / Q000737:chemistry*; D053835:Unilamellar Liposomes / Q000737:chemistry
chemical_list: D005452:Fluoresceins; D010755:Organophosphates; D010710:Phosphates; D013501:Surface-Active Agents; D053835:Unilamellar Liposomes; C024098:6-carboxyfluorescein; C011084:n-decyl phosphoric acid