title: Hepatoprotective activity of LC-ESI-MS standardized Iris spuria rhizome extract on its main bioactive constituents.
abstract: The study was designed to evaluate the hepatoprotective activity of Iris spuria against paracetamol induced toxicity at two different doses 100 and 200 mg/kg. The extract showed significant protective activity (p>0.01) at both the doses in dose dependent manner. Administration of the plant extract restored the paracetamol induced elevated levels of serum marker and distorted hepatic tissue architecture. The lipid peroxides (LPO) and glutathione (GSH) levels were also restored towards normal in liver tissue significantly. The main chemical constituents of the extract identified by the liquid chromatography-tandem mass spectrometry (LC-ESI-MSMS) were found to be flavones and isoflavonoids. Tectoridin and iristectorigenin A were the principal compounds present in the methanolic extract of Iris spuria.
authors.affiliation: Indian Institute of Integrative Medicine, CSIR, Sanat Nagar, Srinagar, Jammu and Kashmir 190005, India. Electronic address: nayeema00@gmail.com.
authors.forename: Nayeema
authors.initials: N
authors.lastname: Akther
authors.affiliation: Department of Biotechnology, University of Kashmir, Srinagar, Jammu and Kashmir, India.
authors.forename: Khursheed
authors.initials: K
authors.lastname: Andrabi
authors.affiliation: Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India.
authors.forename: Albeena
authors.initials: A
authors.lastname: Nissar
authors.affiliation: Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India.
authors.forename: Showkat
authors.initials: S
authors.lastname: Ganaie
authors.affiliation: Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India.
authors.forename: B K
authors.initials: BK
authors.lastname: Chandan
authors.affiliation: Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India.
authors.forename: A P
authors.initials: AP
authors.lastname: Gupta
authors.affiliation: Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Khuswant
authors.affiliation: Department of Medicinal and Elemental Toxicology, Faculty of Science, Jamia Hamdard, New Delhi 110062, India.
authors.forename: Sarwat
authors.initials: S
authors.lastname: Sultana
authors.affiliation: Indian Institute of Integrative Medicine, CSIR, Sanat Nagar, Srinagar, Jammu and Kashmir 190005, India. Electronic address: asshawl@gmail.com.
authors.forename: Abdul S
authors.initials: AS
authors.lastname: Shawl
mesh_terms: D000082:Acetaminophen / Q000633:toxicity*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000503:physiopathology; D002853:Chromatography, Liquid; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D031323:Iris Plant / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D027343:Rhizome / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D010936:Plant Extracts; D000082:Acetaminophen; D005978:Glutathione
keywords: Hepatoprotective; Iris spuria; Isoflavonoids; Paracetamol; Tectoridin