title: Development of an ex vivo porcine lung model for studying growth, virulence, and signaling of Pseudomonas aeruginosa.
abstract: Research into chronic infection by bacterial pathogens, such as Pseudomonas aeruginosa, uses various in vitro and live host models. While these have increased our understanding of pathogen growth, virulence, and evolution, each model has certain limitations. In vitro models cannot recapitulate the complex spatial structure of host organs, while experiments on live hosts are limited in terms of sample size and infection duration for ethical reasons; live mammal models also require specialized facilities which are costly to run. To address this, we have developed an ex vivo pig lung (EVPL) model for quantifying Pseudomonas aeruginosa growth, quorum sensing (QS), virulence factor production, and tissue damage in an environment that mimics a chronically infected cystic fibrosis (CF) lung. In a first test of our model, we show that lasR mutants, which do not respond to 3-oxo-C(12)-homoserine lactone (HSL)-mediated QS, exhibit reduced virulence factor production in EVPL. We also show that lasR mutants grow as well as or better than a corresponding wild-type strain in EVPL. lasR mutants frequently and repeatedly arise during chronic CF lung infection, but the evolutionary forces governing their appearance and spread are not clear. Our data are not consistent with the hypothesis that lasR mutants act as social "cheats" in the lung; rather, our results support the hypothesis that lasR mutants are more adapted to the lung environment. More generally, this model will facilitate improved studies of microbial disease, especially studies of how cells of the same and different species interact in polymicrobial infections in a spatially structured environment.
authors.affiliation: School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom freya.harrison@nottingham.ac.uk.
authors.forename: Freya
authors.initials: F
authors.lastname: Harrison
authors.affiliation: School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom.
authors.forename: Aneesha
authors.initials: A
authors.lastname: Muruli
authors.affiliation: School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom.
authors.forename: Steven
authors.initials: S
authors.lastname: Higgins
authors.affiliation: School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom.
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Diggle
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D066298:In Vitro Techniques; D008168:Lung / Q000382:microbiology* / Q000473:pathology*; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity* / Q000502:physiology; D053038:Quorum Sensing; D013552:Swine; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; C080365:LasR protein, Pseudomonas aeruginosa; D015534:Trans-Activators