title: Identification of quantitative trait loci for abscisic acid responsiveness in the D-genome of hexaploid wheat.
abstract: In crop species such as wheat, abiotic stresses and preharvest sprouting reduce grain yield and quality. The plant hormone abscisic acid (ABA) plays important roles in abiotic stress tolerance and seed dormancy. In previous studies, we evaluated ABA responsiveness of 67 Aegilops tauschii accessions and their synthetic hexaploid wheat lines, finding wide variation that was due to the D-genome. In this study, quantitative trait locus (QTL) analysis was performed using an F2 population derived from crosses of highly ABA-responsive and less-responsive synthetic wheat lines. A significant QTL was detected on chromosome 6D, in a similar location to that reported for ABA responsiveness using recombinant inbred lines derived from common wheat cultivars Mironovskaya 808 and Chinese Spring. A comparative map and physiological and expression analyses of the 6D QTL suggested that this locus involved in line differences among wheat synthetics is different from that involved in cultivar differences in common wheat. The common wheat 6D QTL was found to affect seed dormancy and the regulation of cold-responsive/late embryogenesis abundant genes during dehydration. However, in synthetic wheat, we failed to detect any association of ABA responsiveness with abiotic stress tolerance or seed dormancy, at least under our experimental conditions. Development of near-isogenic lines will be important for functional analyses of the synthetic wheat 6D QTL.
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Julio C M
authors.initials: JC
authors.lastname: Iehisa
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
authors.forename: Takakazu
authors.initials: T
authors.lastname: Matsuura
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
authors.forename: Izumi C
authors.initials: IC
authors.lastname: Mori
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yokota
authors.affiliation: Plant Genome Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe 657-8501, Japan. Electronic address: takumi@kobe-u.ac.jp.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takumi
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D002453:Cell Cycle; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D003681:Dehydration; D002523:Edible Grain / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D018745:Genome, Plant / Q000235:genetics*; D018525:Germination; D057445:Plant Dormancy / Q000235:genetics; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011123:Polyploidy; D040641:Quantitative Trait Loci / Q000235:genetics*; D055049:Salt Tolerance; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D013312:Stress, Physiological; D014908:Triticum / Q000235:genetics* / Q000254:growth & development / Q000502:physiology
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D000040:Abscisic Acid
keywords: ABA; Dehydration tolerance; Quantitative trait locus; Synthetic hexaploid wheat; Triticum aestivum