title: Effects of ubiquinol with fluid resuscitation following haemorrhagic shock on rat lungs, diaphragm, heart and kidneys.
abstract: Haemorrhagic shock (HS) and fluid resuscitation can lead to increased reactive oxygen species (ROS), contributing to ischaemia-reperfusion injury and organ damage. Ubiquinol is a potent antioxidant that decreases ROS. This study examined the effects of ubiquinol administered with fluid resuscitation following controlled HS. Adult male Sprague-Dawley rats were randomly assigned to treatment [ubiquinol, 1 mg (100 g body weight)(-1)] or control groups. Rats were subjected to 60 min of HS by removing 40% of the total blood volume to a mean arterial pressure ∼45-55 mmHg. The animals were resuscitated with blood and lactated Ringer solution, with or without ubiquinol, and monitored for 120 min. At the end of the experiments, the rats were killed and the lungs, diaphragm, heart and kidneys harvested. Leucocytes were analysed for mitochondrial superoxide at baseline, end of shock and 120 min following fluid resuscitation using MitoSOX Red. Diaphragms were examined for hydrogen peroxide using dihydrofluorescein diacetate and confocal microscopy. The apoptosis in lungs, diaphragm, heart and kidneys was measured using fluorescence microscopy with acridine orange and ethidium bromide. Leucocyte mitochondrial superoxide levels were significantly lower in rats that received ubiquinol than in the control animals. Production of hydrogen peroxide and apoptosis were significantly reduced in the organs of rats treated with ubiquinol. These findings suggest that ubiquinol, administered with fluid resuscitation after HS, attenuates ROS production and apoptosis. Thus, ubiquinol is a potent antioxidant that may be used as a potential treatment to reduce organ injury following haemorrhagic events.
authors.affiliation: Department of Nurse Anesthesia Education, University of Kansas, Kansas City, KS, 66160, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Bennetts
authors.affiliation: School of Nursing, University of Kansas, Kansas City, KS, 66160, USA qshen@kumc.edu.
authors.forename: Qiuhua
authors.initials: Q
authors.lastname: Shen
authors.affiliation: School of Nursing, University of Kansas, Kansas City, KS, 66160, USA.
authors.forename: Amanda R
authors.initials: AR
authors.lastname: Thimmesch
authors.affiliation: Department of Biostatistics, University of Kansas, Kansas City, KS, 66160, USA.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Diaz
authors.affiliation: Department of Molecular and Integrative Physiology, University of Kansas, Kansas City, KS, 66160, USA.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Clancy
authors.affiliation: School of Nursing, University of Kansas, Kansas City, KS, 66160, USA.
authors.forename: Janet D
authors.initials: JD
authors.lastname: Pierce
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D003964:Diaphragm / Q000187:drug effects / Q000473:pathology; D005440:Fluid Therapy; D006321:Heart / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D008928:Mitochondria / Q000187:drug effects; D009206:Myocardium / Q000473:pathology; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy*; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000188:drug therapy*; D013481:Superoxides / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D013481:Superoxides; D014451:Ubiquinone; D006861:Hydrogen Peroxide; C003741:ubiquinol