title: Xylanolytic enzyme systems in Arthrobacter sp. MTCC 5214 and Lactobacillus sp.
abstract: The production of extracellular xylanolytic enzymes such as xylanase, α-l-arabinofuranosidase (α-l-AFase), and acetyl xylan esterase (Axe) by marine Arthrobacter sp. and Lactobacillus sp. was investigated using different carbon sources. Induction and repression of all these enzymes differed with carbon source and also with the organism. Wheat bran was the best carbon source for the production of xylanase and α-l-AFase, whereas both isolates showed maximum Axe production when grown on oat bran as a carbon source. Preferential utilization of a carbon source for enzyme production can give us better insights into regulatory mechanism in these marine bacteria. Elution profile as well as zymogram analysis indicated the possibility of bifunctional α-l-AFase-Axes in both marine bacteria.
authors.affiliation: National Institute of Oceanography, CSIR, Goa, India.
authors.forename: Rakhee
authors.initials: R
authors.lastname: Khandeparker
authors.forename: Thasneem
authors.initials: T
authors.lastname: Jalal
mesh_terms: D001173:Arthrobacter / Q000145:classification / Q000201:enzymology*; D002244:Carbon / Q000378:metabolism*; D004043:Dietary Fiber / Q000382:microbiology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D007778:Lactobacillus / Q000145:classification / Q000201:enzymology*; D013045:Species Specificity; D014995:Xylosidases / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D004043:Dietary Fiber; D002244:Carbon; D014995:Xylosidases
keywords: acetyl xylan esterase; bacteria; marine; xylanase; α-l-arabinofuranosidase