title: Inhibitory effects of a cured antibacterial bonding system on viability and metabolic activity of oral bacteria.
abstract: OBJECTIVE
  To evaluate the antimicrobial efficacy of Clearfil SE Protect (CP) and Clearfil SE Bond (CB) after curing and rinsed against five individual oral microorganisms as well as a mixture of bacterial culture prepared from the selected test organisms.
  
  
  METHODS
  Bacterial suspensions were prepared from single species of Streptococcus mutans, Streptococcus sobrinus, Streptococcus gordonii, Actinomyces viscosus and Lactobacillus lactis, as well as mixed bacterial suspensions from these organisms. Dentin bonding system discs (6 mm×2 mm) were prepared, cured, washed and placed on the bacterial suspension of single species or multispecies bacteria for 15, 30 and 60 min. MTT, Live/Dead bacterial viability (antibacterial effect), and XTT (metabolic activity) assays were used to test the two dentin system's antibacterial effect. All assays were done in triplicates and each experiment repeated at least three times. Data were submitted to ANOVA and Scheffe's f-test (5%).
  
  
  RESULTS
  Greater than 40% bacteria killing was seen within 15 min, and the killing progressed with increasing time of incubation with CP discs. However, a longer (60 min) period of incubation was required by CP to achieve similar antimicrobial effect against mixed bacterial suspension. CB had no significant effect on the viability or metabolic activity of the test microorganisms when compared to the control bacterial culture. CP was significantly effective in reducing the viability and metabolic activity of the test organisms.
  
  
  CONCLUSIONS
  The results demonstrated the antimicrobial efficacy of CP both on single and multispecies bacterial culture. CP may be beneficial in reducing bacterial infections in cavity preparations in clinical dentistry.
authors.affiliation: Pediatric Dentistry Division, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, São Paulo, Brazil.
authors.forename: Éfani C F
authors.initials: ÉC
authors.lastname: Banzi
authors.affiliation: Dental Materials Division, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, São Paulo, Brazil.
authors.forename: Ana R
authors.initials: AR
authors.lastname: Costa
authors.affiliation: Pediatric Dentistry and Dental Materials Division, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, São Paulo, Brazil. Electronic address: rmpuppin@fop.unicamp.br.
authors.forename: Regina M
authors.initials: RM
authors.lastname: Puppin-Rontani
authors.affiliation: Department of Bioscience Research, University of Tennessee Health Science Center, College of Dentistry, Memphis, TN, USA.
authors.forename: Jegdish
authors.initials: J
authors.lastname: Babu
authors.affiliation: Department of Bioscience Research, Bioscience Research Center, University of Tennessee Health Science Center, College of Dentistry, Memphis, TN, USA.
authors.forename: Franklin
authors.initials: F
authors.lastname: García-Godoy
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001419:Bacteria / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D015169:Colony Count, Microbial; D001840:Dental Bonding; D006801:Humans; D009055:Mouth / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibacterial dentin bonding system; Cariogenic bacteria; Live/Dead assay; MTT assay; Multi-species culture; XTT assay