title: Expression of aquaporins in the lungs of mice with acute injury caused by LPS treatment.
abstract: The aim of the present study was to investigate the expression of aquaporin 1 (AQP1) and AQP5 in the lungs of mice with acute injury induced by LPS treatment. In the study, the concentrations of cytokines were all significantly increased in the BALF of mice received LPS at 12h and 24h (P<0.001). The lung wet/dry weight ratios (W/D) and total protein content in BALF were also increased in the mice treated with LPS (P<0.001). Interestingly the expression of AQP1 and AQP5 was significantly decreased (P<0.05) compared with these in the control mice, while TUNEL positive cells were increased. However, the AQP5 expression was significantly higher at 24h that it at 12h in the control mice. Our results showed that decreased AQP expression was associated with the increased inflammatory factors, as well as apoptotic cells. The increased expression of AQP5 at 24h in control mice might be due to its regulation in transcellular water reabsorption.
authors.affiliation: Laboratory of Pulmonary Physiology and Pathology, Traditional Chinese Medicine Hospital affiliated with Xinjiang Medical University, Urumqi 830011, China; Postdoctoral Research Station of Clinical Medicine, Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Bilal
authors.initials: B
authors.lastname: Hasan
authors.affiliation: Laboratory of Pulmonary Physiology and Pathology, Traditional Chinese Medicine Hospital affiliated with Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Feng-sen
authors.initials: FS
authors.lastname: Li
authors.affiliation: Departments of Pathology, The Third Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Adila
authors.initials: A
authors.lastname: Siyit
authors.affiliation: Laboratory of Pulmonary Physiology and Pathology, Traditional Chinese Medicine Hospital affiliated with Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Ehbal
authors.initials: E
authors.lastname: Tuyghun
authors.affiliation: Department of Histology and Embryology, Basic Medical College, Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Jing-hua
authors.initials: JH
authors.lastname: Luo
authors.affiliation: Postdoctoral Research Station of Clinical Medicine, Xinjiang Medical University, Urumqi 830011, China. Electronic address: halmurat@263.net.
authors.forename: Halmurat
authors.initials: H
authors.lastname: Upur
authors.affiliation: Postdoctoral Research Station of Clinical Medicine, Xinjiang Medical University, Urumqi 830011, China; Department of Histology and Embryology, Basic Medical College, Xinjiang Medical University, Urumqi 830011, China. Electronic address: xukur325@foxmail.com.
authors.forename: Abduxukur
authors.initials: A
authors.lastname: Ablimit
mesh_terms: D000208:Acute Disease; D055371:Acute Lung Injury / Q000473:pathology / Q000503:physiopathology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D051398:Aquaporin 1 / Q000378:metabolism*; D051402:Aquaporin 5 / Q000378:metabolism*; D015153:Blotting, Western; D016207:Cytokines / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008070:Lipopolysaccharides; D008168:Lung / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008807:Mice, Inbred BALB C; D010539:Permeability; D011654:Pulmonary Edema / Q000473:pathology / Q000503:physiopathology
chemical_list: C496021:Aqp1 protein, mouse; C496038:Aqp5 protein, mouse; D051402:Aquaporin 5; D016207:Cytokines; D008070:Lipopolysaccharides; D051398:Aquaporin 1
keywords: Acute lung injury; Aquaporins; Pulmonary edema; Water reabsorption