title: MicroRNA-497 inhibition of ovarian cancer cell migration and invasion through targeting of SMAD specific E3 ubiquitin protein ligase 1.
abstract: Ovarian cancer is the leading cause of death from gynecological malignancies worldwide. Understanding the molecular mechanism underlying ovarian cancer progression facilitates the development of promising strategy for ovarian cancer therapy. Previously, we observed frequent down-regulation of miR-497 expression in ovarian cancer tissues. In this study, we investigated the role of miR-497 in ovarian cancer metastasis. We found that endogenous miR-497 expression was down-regulated in the more aggressive ovarian cancer cell lines compared with the less aggressive cells. Exogenous expression of miR-497 suppressed ovarian cancer cell migration and invasion, whereas reduction of endogenous miR-497 expression induced tumor cell migration and invasion. Mechanistic investigations confirmed pro-metastatic factor SMURF1 as a direct target of miR-497 through which miR-497 ablated tumor cell migration and invasion. Further studies revealed that lower levels of miR-497 expression were associated with shorter overall survival as well as increased SMURF1 expression in ovarian cancer patients. Our results indicate that down-regulation of miR-497 in ovarian cancer may facilitate tumor metastasis. Restoration of miR-497 expression may be a promising strategy for ovarian cancer therapy.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ren
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
authors.forename: Dazhi
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Nuclear Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China; Department of Radiology, Beijing Youan Hospital, Capital Medical University, Beijing 100054, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
authors.forename: Jinglu
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China. Electronic address: huirongshiww@163.com.
authors.forename: Huirong
authors.initials: H
authors.lastname: Shi
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D015536:Down-Regulation; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000502:physiology*; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D010051:Ovarian Neoplasms / Q000473:pathology*; D011379:Prognosis; D044767:Ubiquitin-Protein Ligases / Q000037:antagonists & inhibitors*
chemical_list: C559640:MIRN497 microRNA, human; D035683:MicroRNAs; C498342:SMURF1 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: Invasion; MiR-497; Migration; Ovarian cancer; Prognosis; SMURF1