title: Plant cytokinesis is orchestrated by the sequential action of the TRAPPII and exocyst tethering complexes.
abstract: Plant cytokinesis is initiated in a transient membrane compartment, the cell plate, and completed by a process of maturation during which the cell plate becomes a cross wall. How the transition from juvenile to adult stages occurs is poorly understood. In this study, we monitor the Arabidopsis transport protein particle II (TRAPPII) and exocyst tethering complexes throughout cytokinesis. We show that their appearance is predominantly sequential, with brief overlap at the onset and end of cytokinesis. The TRAPPII complex is required for cell plate biogenesis, and the exocyst is required for cell plate maturation. The TRAPPII complex sorts plasma membrane proteins, including exocyst subunits, at the cell plate throughout cytokinesis. We show that the two tethering complexes physically interact and propose that their coordinated action may orchestrate not only plant but also animal cytokinesis.
authors.affiliation: Botany, Technische Universität München, 85354 Freising, Germany.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Rybak
authors.affiliation: Botany, Technische Universität München, 85354 Freising, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Steiner
authors.affiliation: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Synek
authors.affiliation: Chair for Proteomics and Bioanalytics, Technische Universität München, 85354 Freising, Germany.
authors.forename: Susan
authors.initials: S
authors.lastname: Klaeger
authors.affiliation: Department of Experimental Plant Biology, Faculty of Science, Charles University, 128 44 Prague 2, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Kulich
authors.affiliation: Department Biologie I, Ludwig-Maximillians Universität, Systematische Botanik und Mykologie, 80638 Munich, Germany; Department Biologie I, Ludwig-Maximillians Universität, 82152 Planegg-Martinsried, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Facher
authors.affiliation: Department Biologie I, Ludwig-Maximillians Universität, 82152 Planegg-Martinsried, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wanner
authors.affiliation: Chair for Proteomics and Bioanalytics, Technische Universität München, 85354 Freising, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Kuster
authors.affiliation: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic; Department of Experimental Plant Biology, Faculty of Science, Charles University, 128 44 Prague 2, Czech Republic.
authors.forename: Viktor
authors.initials: V
authors.lastname: Zarsky
authors.affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam, Germany; ARC Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, Parkville, VIC 3010, Australia.
authors.forename: Staffan
authors.initials: S
authors.lastname: Persson
authors.affiliation: Botany, Technische Universität München, 85354 Freising, Germany. Electronic address: farhah@wzw.tum.de.
authors.forename: Farhah F
authors.initials: FF
authors.lastname: Assaad
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D048749:Cytokinesis / Q000502:physiology*; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D005089:Exocytosis / Q000502:physiology; D006132:Growth Plate / Q000166:cytology* / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008958:Models, Molecular; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D033921:Vesicular Transport Proteins; C406327:transport protein particle, TRAPP