title: The effect of tuning cold plasma composition on glioblastoma cell viability.
abstract: Previous research in cold atmospheric plasma (CAP) and cancer cell interaction has repeatedly proven that the cold plasma induced cell death. It is postulated that the reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a major role in the CAP cancer therapy. In this paper, we seek to determine a mechanism of CAP therapy on glioblastoma cells (U87) through an understanding of the composition of the plasma, including treatment time, voltage, flow-rate and plasma-gas composition. In order to determine the threshold of plasma treatment on U87, normal human astrocytes (E6/E7) were used as the comparison cell line. Our data showed that the 30 sec plasma treatment caused 3-fold cell death in the U87 cells compared to the E6/E7 cells. All the other compositions of cold plasma were performed based on this result: plasma treatment time was maintained at 30 s per well while other plasma characteristics such as voltage, flow rate of source gas, and composition of source gas were changed one at a time to vary the intensity of the reactive species composition in the plasma jet, which may finally have various effect on cells reflected by cell viability. We defined a term "plasma dosage" to summarize the relationship of all the characteristics and cell viability.
authors.affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, D.C., United States of America.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Neurosurgery, The George Washington University, Washington, D.C., United States of America.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Sherman
authors.affiliation: Department of Electrical and Computer Engineering, The George Washington University, Washington, D.C., United States of America.
authors.forename: William
authors.initials: W
authors.lastname: Murphy
authors.affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, D.C., United States of America; Head and Neck Cancer Research Division, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Edward
authors.initials: E
authors.lastname: Ratovitski
authors.affiliation: Institute for Advanced Biological and Technical Sciences, USMI, Takoma Park, Maryland, United States of America.
authors.forename: Jerome
authors.initials: J
authors.lastname: Canady
authors.affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, D.C., United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Keidar
mesh_terms: D001253:Astrocytes / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D005909:Glioblastoma / Q000188:drug therapy*; D006801:Humans; D058626:Plasma Gases / Q000494:pharmacology*
chemical_list: D058626:Plasma Gases