title: Prevalence and genotypes of extended spectrum beta-lactamases in Enterobacteriaceae isolated from human stool and chicken meat in Hamburg, Germany.
abstract: Chicken meat has been proposed to constitute a source for extended spectrum beta-lactamase (ESBL)-carrying Enterobacteriaceae that colonize and infect humans. In this study the prevalence of ESBL-producing Enterobacteriaceae in stool samples from ambulatory patients who presented in the emergency department of the University Medical Centre Hamburg-Eppendorf with gastrointestinal complains and in chicken meat samples from the Hamburg region were analysed and compared with respect to ESBL-genotypes, sequence types and antibiotic resistance profiles. Twenty-nine (4.1%) of 707 stool samples and 72 (60%) of 120 chicken meat samples were positive for ESBL-producing Enterobacteriaceae. The distribution of ESBL genes in the stool vs. chicken meat isolates (given as % of total isolates from stool vs. chicken meat) was as follows: CTX-M-15 (38% vs. 0%), CTX-M-14 (17% vs. 6%), CTX-M-1 (17% vs. 69%), SHV-12 (3% vs. 18%) and TEM-52 (3% each). Comparison of ESBL- and multilocus sequence type revealed no correlation between isolates of human and chicken. Furthermore, ESBL-producing E. coli from stool samples were significantly more resistant to fluoroquinolones, aminoglycosides and/or trimethoprim-sulfamethoxazole than chicken isolates. The differences in ESBL-genotypes, sequence types and antibiotic resistance patterns indicate that in our clinical setting chicken meat is not a major contributor to human colonization with ESBL-carrying Enterobacteriaceae.
authors.affiliation: Institute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany. Electronic address: c.belmar-campos@uke.de.
authors.forename: Cristina
authors.initials: C
authors.lastname: Belmar Campos
authors.affiliation: Fen-Lab GmbH, Bergstrasse 14, 20095 Hamburg, Germany.
authors.forename: Ines
authors.initials: I
authors.lastname: Fenner
authors.affiliation: Fen-Lab GmbH, Bergstrasse 14, 20095 Hamburg, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Wiese
authors.affiliation: Fen-Lab GmbH, Bergstrasse 14, 20095 Hamburg, Germany.
authors.forename: Carmen
authors.initials: C
authors.lastname: Lensing
authors.affiliation: Institute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Christner
authors.affiliation: Institute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Rohde
authors.affiliation: Institute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Aepfelbacher
authors.affiliation: Fen-Lab GmbH, Bergstrasse 14, 20095 Hamburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Fenner
authors.affiliation: Institute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
authors.forename: Moritz
authors.initials: M
authors.lastname: Hentschke
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002645:Chickens; D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005260:Female; D005838:Genotype; D005858:Germany; D006801:Humans; D007223:Infant; D008297:Male; D008460:Meat / Q000382:microbiology*; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D015995:Prevalence; D055815:Young Adult; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases
keywords: ESBL; Emergency department; Poultry; Sequence type