title: A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
abstract: Many marine microorganisms can secrete exopolysaccharides (EPSs) which have important applications in biotechnology. We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies. To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed. The EPS yield of batch fermentation in this medium reached 12.1 ± 0.3 g/l. Fed-batch fermentation was conducted and led to an EPS yield of 17.2 ± 0.4 g/l, which represents the highest EPS yield ever reported for a marine bacterium. The EPS was extracted and it displayed good rheological properties, moisture-retention ability, and antioxidant activity. Particularly, its moisture-retention ability is superior to that of other marine bacterial EPSs reported to date. SM-A87 EPS also showed high antioxidant activity. These results suggest that SM-A87 EPS has promising potentials in biotechnology.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, Shandong Province, China.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Sun
authors.forename: Sheng-Bo
authors.initials: SB
authors.lastname: Liu
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Qiao
authors.forename: Xiu-Lan
authors.initials: XL
authors.lastname: Chen
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Pang
authors.forename: Mei
authors.initials: M
authors.lastname: Shi
authors.forename: Xi-Ying
authors.initials: XY
authors.lastname: Zhang
authors.forename: Qi-Long
authors.initials: QL
authors.lastname: Qin
authors.forename: Bai-Cheng
authors.initials: BC
authors.lastname: Zhou
authors.forename: Yu-Zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Xie
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism*; D059001:Aquatic Organisms / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D041965:Flavobacteriaceae / Q000378:metabolism*; D058427:Hygroscopic Agents / Q000302:isolation & purification / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D003470:Culture Media; D058427:Hygroscopic Agents; D011135:Polysaccharides, Bacterial