title: [Development and analytical validation according COFRAC recommendations of pyruvate and ketone body assay on open automated analyser].
abstract: Measurements of pyruvate and ketones bodies (acetoacetate and 3-hydroxybutyrate) are essential to the investigation of intermediary metabolism. Indeed, their blood levels reflect energy balance influenced by nutritional status. This balance can be disturbed in certain diseases such as diabetes and some inherited metabolic disorders. We have developed methods for assays on open automated biochemistry analyser, Konelab 20 XT (ThermoFischer, Whaltham USA), using kits marketed by Sobioda (Montbonnot, France) for pyruvate and Wako Chemicals GmbH (Neuss, Germany) for ketones, on deproteinised blood sample. We have validated the performance of these three quantitative methods using NF EN ISO 15189 (range B) standard criteria. We obtain satisfactory results concerning fidelity (precision measured as within and between batch CVs are respectively less than 7% and less than 6%), measuring ranges (from 7.7 to 228 μmol/L for pyruvate and from 22.6 to 650 μM for total ketone bodies), accuracy (10.4 μmol/L in physiological range for pyruvate and 7.1 μmol/L for 3-hydroxybutyrate) and comparing methods (versus manual assay with spectrophotometry on Uvikon XL). Establishment of reference ranges (35 to 74 μmol/L for pyruvate, less than 100 μM for 3-hydroxybutyrate and less than 44 μmol/L for acetoacetate) and reagents stability study (up to 12 weeks if frozen) have enabled us to finalize method validation and to add these assays to our routine laboratory repertoire.
authors.affiliation: Laboratoire des maladies héréditaires du métabolisme, biochimie génétique et moléculaire, Département de biochimie, toxicologie et pharmacologie, CHU de Grenoble site Nord - Institut de biologie et de pathologie, Grenoble, France.
authors.forename: Lysiane
authors.initials: L
authors.lastname: Boulet
authors.affiliation: Laboratoire des maladies héréditaires du métabolisme, biochimie génétique et moléculaire, Département de biochimie, toxicologie et pharmacologie, CHU de Grenoble site Nord - Institut de biologie et de pathologie, Grenoble, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Van Noolen
authors.affiliation: Laboratoire des maladies héréditaires du métabolisme, biochimie génétique et moléculaire, Département de biochimie, toxicologie et pharmacologie, CHU de Grenoble site Nord - Institut de biologie et de pathologie, Grenoble, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Bosson
authors.affiliation: Laboratoire des maladies héréditaires du métabolisme, biochimie génétique et moléculaire, Département de biochimie, toxicologie et pharmacologie, CHU de Grenoble site Nord - Institut de biologie et de pathologie, Grenoble, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Faure
authors.affiliation: Laboratoire des maladies héréditaires du métabolisme, biochimie génétique et moléculaire, Département de biochimie, toxicologie et pharmacologie, CHU de Grenoble site Nord - Institut de biologie et de pathologie, Grenoble, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Corne
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000090:Acetoacetates / Q000097:blood; D001322:Autoanalysis / Q000295:instrumentation* / Q000706:statistics & numerical data; D006801:Humans; D007657:Ketone Bodies / Q000097:blood*; D019289:Pyruvic Acid / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D013053:Spectrophotometry / Q000295:instrumentation
chemical_list: D000090:Acetoacetates; D007657:Ketone Bodies; D011933:Reagent Kits, Diagnostic; C016635:acetoacetic acid; D019289:Pyruvic Acid; D020155:3-Hydroxybutyric Acid
keywords: 3-hydroxybutyrate; aceto-acetate; automated method; pyruvate; validation