title: Coilin is rapidly recruited to UVA-induced DNA lesions and γ-radiation affects localized movement of Cajal bodies.
abstract: Cajal bodies are important nuclear structures containing proteins that preferentially regulate RNA-related metabolism. We investigated the cell-type specific nuclear distribution of Cajal bodies and the level of coilin, a protein of Cajal bodies, in non-irradiated and irradiated human tumor cell lines and embryonic stem (ES) cells. Cajal bodies were localized in different nuclear compartments, including DAPI-poor regions, in the proximity of chromocenters, and adjacent to nucleoli. The number of Cajal bodies per nucleus was cell cycle-dependent, with higher numbers occurring during G2 phase. Human ES cells contained a high coilin level in the nucleoplasm, but coilin-positive Cajal bodies were also identified in nuclei of mouse and human ES cells. Coilin, but not SMN, recognized UVA-induced DNA lesions, which was cell cycle-independent. Treatment with γ-radiation reduced the localized movement of Cajal bodies in many cell types and GFP-coilin fluorescence recovery after photobleaching was very fast in nucleoplasm in comparison with GFP-coilin recovery in DNA lesions. By contrast, nucleolus-localized coilin displayed very slow fluorescence recovery after photobleaching, which indicates very slow rates of protein diffusion, especially in nucleoli of mouse ES cells.
authors.affiliation: Institute of Biophysics; Academy of Sciences of the Czech Republic; Brno, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Bártová
authors.affiliation: Institute of Biophysics; Academy of Sciences of the Czech Republic; Brno, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Foltánková
authors.affiliation: Institute of Biophysics; Academy of Sciences of the Czech Republic; Brno, Czech Republic.
authors.forename: Soňa
authors.initials: S
authors.lastname: Legartová
authors.affiliation: Institute of Biophysics; Academy of Sciences of the Czech Republic; Brno, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Sehnalová
authors.affiliation: Faculty of Informatics; Masaryk University; Brno, Czech Republic.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Sorokin
authors.affiliation: Institute of Biophysics; Academy of Sciences of the Czech Republic; Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Suchánková
authors.affiliation: Institute of Biophysics; Academy of Sciences of the Czech Republic; Brno, Czech Republic.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Kozubek
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D020541:Coiled Bodies / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D004247:DNA / Q000235:genetics* / Q000528:radiation effects*; D016195:G2 Phase / Q000235:genetics; D005720:Gamma Rays / Q000009:adverse effects*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C068921:p80-coilin; D049452:Green Fluorescent Proteins; D004247:DNA
keywords: Cajal bodies; DNA repair; chromatin; coilin; nucleolus; nucleus