title: Rapid spread and diversification of respiratory syncytial virus genotype ON1, Kenya.
abstract: Respiratory syncytial virus genotype ON1, which is characterized by a 72-nt duplication in the attachment protein gene, has been detected in >10 countries since first identified in Ontario, Canada, in 2010. We describe 2 waves of genotype ON1 infections among children admitted to a rural hospital in Kenya during 2012. Phylogenetic analysis of attachment protein gene sequences showed multiple introductions of genotype ON1; variants distinct from the original Canadian viruses predominated in both infection waves. The genotype ON1 dominated over the other group A genotypes during the second wave, and some first wave ON1 variants reappeared in the second wave. An analysis of global genotype ON1 sequences determined that this genotype has become considerably diversified and has acquired signature coding mutations within immunogenic regions, and its most recent common ancestor dates to ≈2008-2009. Surveillance of genotype ON1 contributes to an understanding of the mechanisms of rapid emergence of respiratory viruses.
authors.forename: Charles N
authors.initials: CN
authors.lastname: Agoti
authors.forename: James R
authors.initials: JR
authors.lastname: Otieno
authors.forename: Caroline W
authors.initials: CW
authors.lastname: Gitahi
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Cane
authors.forename: D James
authors.initials: DJ
authors.lastname: Nokes
mesh_terms: D000595:Amino Acid Sequence; D002675:Child, Preschool; D005260:Female; D005814:Genes, Viral; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D007630:Kenya / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000635:transmission / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000145:classification / Q000235:genetics*; D012621:Seasons; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C116204:attachment protein G
keywords: G glycoprotein; G protein gene; Kenya; ON1; RSV; attachment protein; epidemics; genotype ON1; humans; phylogenetic analysis; pneumonia; respiratory syncytial virus; respiratory viruses; surveillance; viruses