title: A comparison of protein profiles of cervical tissue homogenate, exfoliated cells from cervix and serum in normal and cervical malignancy conditions.
abstract: Cervical cancer, the second most common cancer in women, progresses silently over long periods before producing any clinical manifestation. Research in early detection of this condition using proteomic techniques is of very recent origin. We used high-performance liquid chromatography combined with laser-induced fluorescence method in our lab to record the protein profiles of tissue homogenate, cell lysate and serum samples of normal and different stages of malignant conditions of the cervix. Information on protein markers in the protein profiles was derived using various data processing methods including curve resolution. The variations in relative intensities of different peaks with respect to peak height, width and area under the curve from different sample types were compared to get information regarding the concentration of the various proteins and their significance in the induction and metastasis of cervical cancer. The method can be used in diagnosis, follow-up with respect to the progression, remission and effective therapy, in cervical malignancy.
authors.affiliation: KMC International Center, Manipal University, Manipal, Karnataka 576104, India.
authors.forename: Sujatha
authors.initials: S
authors.lastname: Bhat
authors.affiliation: 21 Udayagiri, Sion Trombay Road, Deonar, Mumbai 400088, India.
authors.forename: Vasudevan Bhaskaran
authors.initials: VB
authors.lastname: Kartha
authors.affiliation: Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, Karnataka 576104, India.
authors.forename: Lavanya
authors.initials: L
authors.lastname: Rai
authors.affiliation: Centre for Atomic and Molecular Physics, Manipal University, Academic Block-5, MIT Campus, Manipal, Karnataka 576104, India santhosh.cls@manipal.edu santhosh.cls@gmail.com.
authors.forename: Santhosh
authors.initials: S
authors.lastname: Chidangil
mesh_terms: D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D002584:Cervix Uteri / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D005260:Female; D006801:Humans; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D044967:Serum / Q000737:chemistry*; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology
chemical_list: D015415:Biomarkers; D011506:Proteins