title: Transcriptome analysis of shade-induced inhibition on leaf size in relay intercropped soybean.
abstract: Multi-species intercropping is a sustainable agricultural practice worldwide used to utilize resources more efficiently. In intercropping systems, short crops often grow under vegetative shade of tall crops. Soybean, one important legume, is often planted in intercropping. However, little is known about the mechanisms of shade inhibition effect on leaf size in soybean leaves at the transcriptome level. We analyzed the transcriptome of shaded soybean leaves via RNA-Seq technology. We found that transcription 1085 genes in mature leaves and 1847 genes in young leaves were significantly affected by shade. Gene ontology analyses showed that expression of genes enriched in polysaccharide metabolism was down-regulated, but genes enriched in auxin stimulus were up-regulated in mature leaves; and genes enriched in cell cycling, DNA-replication were down-regulated in young leaves. These results suggest that the inhibition of higher auxin content and shortage of sugar supply on cell division and cell expansion contribute to smaller and thinner leaf morphology, which highlights potential research targets such as auxin and sugar regulation on leaves for crop adaptation to shade in intercropping.
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China.
authors.forename: Wanzhuo
authors.initials: W
authors.lastname: Gong
authors.affiliation: Triticeae Research Institute of Sichuan Agricultural University, Chengdu, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Qi
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China.
authors.forename: Junbo
authors.initials: J
authors.lastname: Du
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China; College of Resource and Environment, Sichuan Agricultural University, Chengdu, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Song
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China.
authors.forename: Yushan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China.
authors.forename: Taiwen
authors.initials: T
authors.lastname: Yong
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Yan
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Yang
mesh_terms: D004261:DNA Replication; D017343:Genes, Plant; D010788:Photosynthesis; D018515:Plant Leaves; D016133:Polymerase Chain Reaction; D011134:Polysaccharides / Q000378:metabolism; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D011134:Polysaccharides