title: Influence of open testicular biopsy in prepubertal rats on rats' adulthood fertility with correlation to serum levels of inhibin B and follicle stimulating hormone.
abstract: OBJECTIVE
  Open testicular biopsy (OTB) is one of the options to accurately assess fertility potential of the undescended testis. The aim of the study was to investigate consequences of OTB in prepubertal rats on their adulthood fertility.
  
  
  METHODS
  Thirty-eight prepubertal male rats were divided into three groups depending on day 20 procedure. The first group was the control group, the second sham operated and the third has left OTB. Bilateral orchiectomy was performed on day 70 to all groups, with determination of serum inhibin B and follicle stimulating hormone (FSH). Removed testes were compared according to the weight, volume, spermatogenesis, histological and apoptotic changes in both testes with differences in serum levels of inhibin B and FSH.
  
  
  RESULTS
  Ipsilateral testicular weight, volume, and spermatogenesis reduction with a reduction of tubular number, diameter and germinative epithelium was found in OTB group. Significant increase in apoptotic index was found in biopsied testis without compensatory hypertrophy of contralateral testis. Differences of inhibin B and FSH were not statistically significant among three groups.
  
  
  CONCLUSIONS
  OTB in prepubertal rats has detrimental effects on fertility in adulthood. It does not cause compensatory hypertrophy of the contralateral testis nor does it disturb serum levels of inhibin B and FSH.
authors.affiliation: Department of Pediatric Surgery, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000 Split, Croatia.
authors.forename: Davor
authors.initials: D
authors.lastname: Todorić
authors.affiliation: Department of Pathology, Forensic Medicine and Cytology, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000 Split, Croatia.
authors.forename: Merica Glavina
authors.initials: MG
authors.lastname: Durdov
authors.affiliation: Department of Gynecology, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000 Split, Croatia.
authors.forename: Marijan
authors.initials: M
authors.lastname: Tandara
authors.affiliation: Department of Nuclear Medicine, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000 Split, Croatia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Čapkun
authors.affiliation: Department of Pediatric Surgery, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000 Split, Croatia.
authors.forename: Ivo
authors.initials: I
authors.lastname: Jurić
authors.affiliation: Department of Pediatric Surgery, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000 Split, Croatia.
authors.forename: Mihovil
authors.initials: M
authors.lastname: Biočić
authors.affiliation: Department of Pediatric Surgery, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000 Split, Croatia.
authors.forename: Jakov
authors.initials: J
authors.lastname: Meštrović
authors.affiliation: Department of Pediatric Surgery, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000 Split, Croatia. Electronic address: zenon@vip.hr.
authors.forename: Zenon
authors.initials: Z
authors.lastname: Pogorelić
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D003456:Cryptorchidism / Q000097:blood / Q000473:pathology*; D004195:Disease Models, Animal; D005298:Fertility / Q000502:physiology*; D005640:Follicle Stimulating Hormone / Q000097:blood*; D007265:Inhibins / Q000097:blood*; D008297:Male; D011627:Puberty / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; C422364:inhibin B; D007265:Inhibins; D005640:Follicle Stimulating Hormone
keywords: Adulthood fertility; Follicle stimulating hormone; Inhibin B; Open testicular biopsy; Prepubertal rats