title: Leukocyte phagocytosis and lysozyme activity in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis.
abstract: Although there is evidence on the benefits in the use of immunostimulants in aquaculture, there are few commercial products being used. This study evaluated the use of natural substances as potential sources for the production of immunostimulants. Propolis and Aloe barbadensis have been widely studied and its extracts have different chemical constituents responsible for antimicrobial, anti-inflammatory and immunostimulant. Tilapia juveniles were fed for two weeks with diets supplemented mix of propolis extracts and aloe (1:1) in different concentrations: 0.5, 1 e 2%. After the experimental period, fish blood was collected for hematoimmunological as follows : hematocrit, total plasma protein, erythrocytes (RBC), leukocytes (WBC), differential leukocyte count, phagocytic activity, serum lysozyme activity, and serum antimicrobial activity, serum antimicrobial activity (evaluated against Aeromonas hydrophila, Enterococcus durans and Escherichia coli). Except for higher number of thrombocytes in 1%-supplemented fish, the rest did not show significant difference.
authors.affiliation: AQUOS Laboratory - Health of Aquatic Organisms, Department of Aquaculture, Center for Agricultural Sciences, Federal University of Santa Catarina (UFSC), Rod Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Geovana
authors.initials: G
authors.lastname: Dotta
authors.affiliation: AQUOS Laboratory - Health of Aquatic Organisms, Department of Aquaculture, Center for Agricultural Sciences, Federal University of Santa Catarina (UFSC), Rod Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Jaqueline Inês Alves
authors.initials: JI
authors.lastname: de Andrade
authors.affiliation: AQUOS Laboratory - Health of Aquatic Organisms, Department of Aquaculture, Center for Agricultural Sciences, Federal University of Santa Catarina (UFSC), Rod Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Eduardo Luiz
authors.initials: EL
authors.lastname: Tavares Gonçalves
authors.affiliation: AQUOS Laboratory - Health of Aquatic Organisms, Department of Aquaculture, Center for Agricultural Sciences, Federal University of Santa Catarina (UFSC), Rod Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Brum
authors.affiliation: Laboratory for Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, CCB, UFSC, Brazil.
authors.forename: Jacó Joaquim
authors.initials: JJ
authors.lastname: Mattos
authors.affiliation: Laboratory of Plant Morphogenesis and Biochemistry, Department of Plant Science, CCA, UFSC, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Maraschin
authors.affiliation: AQUOS Laboratory - Health of Aquatic Organisms, Department of Aquaculture, Center for Agricultural Sciences, Federal University of Santa Catarina (UFSC), Rod Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil. Electronic address: mauricio.martins@ufsc.br.
authors.forename: Maurício Laterça
authors.initials: ML
authors.lastname: Martins
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D000504:Aloe / Q000737:chemistry*; D000704:Analysis of Variance; D000818:Animals; D017756:Aquaculture / Q000379:methods; D023681:Cichlids / Q000276:immunology*; D019587:Dietary Supplements; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011429:Propolis / Q000008:administration & dosage / Q000494:pharmacology*; D015610:Serum Bactericidal Test / Q000662:veterinary
chemical_list: D000276:Adjuvants, Immunologic; D010936:Plant Extracts; D011429:Propolis
keywords: Fish; Hematology; Immunology; Natural extract; Supplementation