title: Comparison of oxidative status of mouse pre-antral follicles derived from vitrified whole ovarian tissue and vitrified pre-antral follicles in the presence of alpha lipoic acid.
abstract: OBJECTIVE
  The main goal of this study was to compare developmental competence and oxidative status of vitrified-warmed pre-antral follicles (VPF) with pre-antral follicles derived from vitrified-warmed ovarian tissue (VOF) in the presence of alpha lipoic acid (ALA).
  
  
  METHODS
  Ovarian tissue and isolated pre-antral follicles were exposed to equilibration solution and then vitrification solution. After thawing of LN2 snap-frozen samples, pre-antral follicles were cultured with or without ALA for 12 days that followed by hCG-induced ovulation. MII oocytes were in vitro fertilized and embryo cleavage assessed. Reactive oxygen species (ROS) and total antioxidant capacity (TAC) levels of cultured pre-antral follicles were measured.
  
  
  RESULTS
  The rates of survival, antral-like cavity formation, MII oocytes, fertilization, 2-cell embryo and blastocyst development were higher in VPF compared to VOF. These rates were higher in ALA-supplemented groups in comparison to their respective groups. An increase and a decrease in ROS production and TAC levels were observed up to the 96 h during cultivation period, respectively. ROS level was lower in cultured VPF compared to VOF. In ALA-treated groups, ROS level decreased to reach comparable values of starting point and TAC levels increased after 24 h of culture and then remained constant.
  
  
  CONCLUSIONS
  Developmental outcomes showed vitrification of pre-antral follicles is more appropriate method than that of whole ovarian tissue. Moreover, it seems that inclusion of ALA improved in vitro development of pre-antral follicles in both vitrified and non-vitrified samples.
authors.affiliation: School of Biology, Damghan University, Damghan, Islamic Republic of Iran.
authors.forename: Sahar
authors.initials: S
authors.lastname: Hatami
authors.forename: Saeed
authors.initials: S
authors.lastname: Zavareh
authors.forename: Mojdeh
authors.initials: M
authors.lastname: Salehnia
authors.forename: Taghi
authors.initials: T
authors.lastname: Lashkarbolouki
authors.forename: Isaac
authors.initials: I
authors.lastname: Karimi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D015925:Cryopreservation; D047108:Embryonic Development; D005260:Female; D005306:Fertilization; D051379:Mice; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D008063:Thioctic Acid / Q000494:pharmacology*; D058989:Vitrification
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D008063:Thioctic Acid
keywords: alpha lipoic acid; ovarian tissue; oxidative status; vitrification