title: Generation of an infectious Negev virus cDNA clone.
abstract: The genus Negevirus consists of insect-only viruses isolated from mosquitoes and sandflies. Here, we report the successful construction of a full-length infectious cDNA clone of Negev virus (NEGV) strain M30957. Viral RNA was transcribed in vitro and virus was readily rescued with or without the use of a cap analogue. These results strongly suggest that NEGV, and likely other members within the genus, is a non-segmented, single-stranded, positive-sense RNA virus.
authors.affiliation: Institute for Human Infections and Immunity, Center for Tropical Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
authors.forename: Rodion V
authors.initials: RV
authors.lastname: Gorchakov
authors.affiliation: Institute for Human Infections and Immunity, Center for Tropical Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Tesh
authors.affiliation: Institute for Human Infections and Immunity, Center for Tropical Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
authors.forename: Scott C
authors.initials: SC
authors.lastname: Weaver
authors.affiliation: Institute for Human Infections and Immunity, Center for Tropical Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
authors.forename: Farooq
authors.initials: F
authors.lastname: Nasar
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D016679:Genome, Viral; D007304:Insect Viruses / Q000235:genetics*; D007313:Insecta / Q000821:virology*; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral