title: Anti-amnesic effect of alkaloid fraction from Lycopodiella cernua (L.) Pic. Serm. on scopolamine-induced memory impairment in mice.
abstract: Lycopodiella cernua (L.) Pic. Serm. (Licopodiaceae) has been used in Vietnamese folk medicine for treating central nervous system conditions. In this study, the alkaloid fraction from the methanol extract of this plant (VLC) was evaluated for in vitro acetylcholinesterase (AChE) inhibitory activity in cognition-relevant brain areas of mice. In in vivo study, the cognitive-enhancing effect of VLC on amnesic mice induced by scopolamine was investigated by assessing a passive avoidance and a Morris water maze test. VLC inhibited AChE activity in mouse frontal cortex, hippocampus and striatum with IC50 values of 26.7, 32.2 and 25.7μg/mL, respectively. Administration of VLC (10, 20, 50 and 100mg/kg, p.o.) significantly reversed cognitive impairments in mice by passive avoidance test. Treating with VLC (50mg/kg) reduced escape latencies in training trials and prolonged swimming times in the target quadrant during the probe trial in the water maze task (P<0.05). These results indicated that L. cernua originated from Vietnam has anti-cholinesterase activity and might be useful for the treatment of cognitive impairment.
authors.affiliation: Research Center of Ginseng and Medicinal Materials, 41-Dinh Tien Hoang, District 1, Ho Chi Minh City, Viet Nam.
authors.forename: Nguyen Ngoc
authors.initials: NN
authors.lastname: Chuong
authors.affiliation: Faculty of Chemistry, University of Science, Vietnam National University-Ho Chi Minh City, 227 Nguyen Van Cu, District 5, Ho Chi Minh City, Viet Nam.
authors.forename: Bui Huu
authors.initials: BH
authors.lastname: Trung
authors.affiliation: Research Center of Ginseng and Medicinal Materials, 41-Dinh Tien Hoang, District 1, Ho Chi Minh City, Viet Nam.
authors.forename: Tran Cong
authors.initials: TC
authors.lastname: Luan
authors.affiliation: Faculty of Chemistry, University of Science, Vietnam National University-Ho Chi Minh City, 227 Nguyen Van Cu, District 5, Ho Chi Minh City, Viet Nam; College of Pharmacy, Catholic University of Daegu, Gyeongsan 712-702, South Korea. Electronic address: tmhung801018@gmail.com.
authors.forename: Tran Manh
authors.initials: TM
authors.lastname: Hung
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay District, Hanoi, Viet Nam.
authors.forename: Nguyen Hai
authors.initials: NH
authors.lastname: Dang
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay District, Hanoi, Viet Nam.
authors.forename: Nguyen Tien
authors.initials: NT
authors.lastname: Dat
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000470:Alkaloids / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001921:Brain / Q000201:enzymology; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000627:therapeutic use*; D029542:Lycopodiaceae / Q000737:chemistry*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy* / Q000523:psychology; D000432:Methanol; D018697:Nootropic Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D012601:Scopolamine; D012997:Solvents; D014744:Vietnam
chemical_list: D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D018697:Nootropic Agents; D010936:Plant Extracts; D012997:Solvents; D012601:Scopolamine; D000110:Acetylcholinesterase; D000432:Methanol
keywords: Acetylcholinesterase; Amnesic; Licopodiaceae; Lycopodiella cernua; Scopolamine