title: FHIP and FTS proteins are critical for dynein-mediated transport of early endosomes in Aspergillus.
abstract: The minus end-directed microtubule motor cytoplasmic dynein transports various cellular cargoes, including early endosomes, but how dynein binds to its cargo remains unclear. Recently fungal Hook homologues were found to link dynein to early endosomes for their transport. Here we identified FhipA in Aspergillus nidulans as a key player for HookA (A. nidulans Hook) function via a genome-wide screen for mutants defective in early-endosome distribution. The human homologue of FhipA, FHIP, is a protein in the previously discovered FTS/Hook/FHIP (FHF) complex, which contains, besides FHIP and Hook proteins, Fused Toes (FTS). Although this complex was not previously shown to be involved in dynein-mediated transport, we show here that loss of either FhipA or FtsA (A. nidulans FTS homologue) disrupts HookA-early endosome association and inhibits early endosome movement. Both FhipA and FtsA associate with early endosomes, and interestingly, while FtsA-early endosome association requires FhipA and HookA, FhipA-early endosome association is independent of HookA and FtsA. Thus FhipA is more directly linked to early endosomes than HookA and FtsA. However, in the absence of HookA or FtsA, FhipA protein level is significantly reduced. Our results indicate that all three proteins in the FtsA/HookA/FhipA complex are important for dynein-mediated early endosome movement.
authors.affiliation: Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences-F. Edward Hébert School of Medicine, Bethesda, MD 20814.
authors.forename: Xuanli
authors.initials: X
authors.lastname: Yao
authors.affiliation: School of Plant Sciences, University of Arizona, Tucson, AZ 85721.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences-F. Edward Hébert School of Medicine, Bethesda, MD 20814 xin.xiang@usuhs.edu.
authors.forename: Xin
authors.initials: X
authors.lastname: Xiang
mesh_terms: D001233:Aspergillus nidulans / Q000378:metabolism*; D001692:Biological Transport; D004398:Dyneins / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism*; D005656:Fungal Proteins / Q000502:physiology*; D025301:Hyphae / Q000378:metabolism; D021381:Protein Transport
chemical_list: D005656:Fungal Proteins; D004398:Dyneins