title: Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea.
abstract: The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile of tcdA+/tcdB- and cdtA+/cdtB+ was verified in one C. difficile-positive diarrhea sample and of tcdA+/tcdB+ in three C. difficile-positive nondiarrhea samples. The presence of tcdA+/tcdB+ in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains.
authors.affiliation: Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Victor R C
authors.initials: VR
authors.lastname: Merino
authors.affiliation: Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Viviane
authors.initials: V
authors.lastname: Nakano
authors.affiliation: Veterans Affairs, West Los Angeles Medical Center, Los Angeles, CA, USA ; Department of Medicine, School of Medicine, University of California, Los Angeles, CA, USA ; Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of California, Los Angeles, CA, USA.
authors.forename: Sydney M
authors.initials: SM
authors.lastname: Finegold
authors.affiliation: Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Mario J
authors.initials: MJ
authors.lastname: Avila-Campos