title: Identification of Atlantic cod (Gadus morhua), ling (Molva molva), and Alaska pollock (Gadus chalcogrammus) by PCR-ELISA using duplex PCR.
abstract: Species-specific PCR-ELISA assays for the identification of Atlantic cod (Gadus morhua), Alaska pollock (Gadus chalcogrammus), and ling (Molva molva) in food products have been developed. The method, comprising a set of primers common to the first two species, a set of primers for M. molva, and a probe for each species, was designed using ND4 and cytochrome b genes as molecular markers. The sensitivity and selectivity were then determined for each assay. These assays were afterward used to analyze DNA extracted from commercial fish products. The presence of the target species was successfully detected in all analyzed samples, demonstrating the applicability of this method to the analysis of food products.
authors.affiliation: Instituto de Investigaciones Marinas (IIM-CSIC) , Eduardo Cabello 6, E-36208 Vigo, Spain.
authors.forename: Ledicia
authors.initials: L
authors.lastname: Taboada
authors.forename: Ana
authors.initials: A
authors.lastname: Sánchez
authors.forename: Amaya
authors.initials: A
authors.lastname: Velasco
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Santaclara
authors.forename: Ricardo I
authors.initials: RI
authors.lastname: Pérez-Martín
authors.forename: Carmen G
authors.initials: CG
authors.lastname: Sotelo
mesh_terms: D000818:Animals; D045303:Cytochromes b / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D048388:Gadiformes / Q000145:classification / Q000235:genetics*; D048389:Gadus morhua / Q000145:classification / Q000235:genetics*; D009245:NADH Dehydrogenase / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017747:Seafood / Q000032:analysis; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004247:DNA; D045303:Cytochromes b; D009245:NADH Dehydrogenase