title: The antileukaemic cell cycle regulatory activities of swainsonine purified from Metarhizium anisopliae fermentation broth.
abstract: Swainsonine is a Metarhizium secondary metabolite known differentially for its specific mannosidase inhibitory, toxic and therapeutic activities. Here, the standard and purified swainsonine from Metarhizium anisopliae fermentation broth were comparatively evaluated for their in situ antileukaemic activities in human promyelocytic cell line, HL-60. Both the standard (IC50 = 6.96 μM) and purified (IC50 = 9.50 μM) compounds inhibited the leukaemic cell proliferation without inflicting cell membrane disruption at 48 h of post-treatment incubation. The DNA cell cycle analysis showed approximately 48.81% and 60.72% of the treated cells arrested in the synthetic phase (S-phase) at 36 and 48 h, respectively, upon treatment with IC50 concentration of the purified swainsonine. However, only 29.62% of cells were arrested in S-phase with standard swainsonine at 48 h, suggesting the comprehensive action of certain other metabolites sharing the similar paradigm of antiproliferative properties in Metarhizium broth extract.
authors.affiliation: a Department of Biotechnology , Indian Institute of Technology Guwahati , Guwahati 781 039 , Assam , India.
authors.forename: Digar
authors.initials: D
authors.lastname: Singh
authors.forename: Gurvinder
authors.initials: G
authors.lastname: Kaur
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D005285:Fermentation; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000188:drug therapy*; D052981:Metarhizium / Q000737:chemistry*; D017026:Swainsonine / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017026:Swainsonine
keywords: Metarhizium; antileukaemic; cell cycle; swainsonine