title: [Roles of adenosine and cytokines in the prostate tissue of rats with acute bacterial prostatitis].
abstract: OBJECTIVE
  To investigate the possible roles of adenosine and the cytokines TNF-alpha and IL-10 in the pathogenesis of acute bacterial prostatitis (ABP) in rats.
  
  
  METHODS
  Forty-eight male Wistar rats were randomly divided into groups A (ABP), B (ABP + theophylline intervention), C (sham) and D (blank control). ABP models were established by injecting Escherichia coli 0157 into the prostate, and those in group B were treated by intraperitoneal injection of theophylline immediately after modeling. At 4 and 14 days, the prostate tissues of the rats were collected for detection of the expressions of TNF-alpha and IL-10 by immunohistochemistry and the concentration of adenosine by high-performance liquid chromatography.
  
  
  RESULTS
  At 4 and 14 days, the concentrations of adenosine were significantly higher in group A ([48.38 +/- 17.27] and [26.54 +/- 11.22] microg/g) than in C ([0.45 +/- 0.25] and [0.46 +/- 0.29] microg/g) and D ([0.41 +/- 0.23] and [0.43 +/- 0.27] microg/g) (P < 0.05), and so were the expressions of TNF-alpha in A (0.23 +/- 0.08 and 0.21 +/- 0.03) than in C (0.07 +/- 0.03 and 0.07 +/- 0.01) and D (0.07 +/- 0.06 and 0.07 +/- 0.06) (P < 0.05), and those of IL-10 in A (0.13 +/- 0.03 and 0.25 +/- 0.01) than in C (0.07 +/- 0.03 and 0.07 +/- 0.03) and D (0.07 +/- 0.01 and 0.07 +/- 0.02) (P < 0.05). Compared with group A, the rats in group B showed significant increases at 4 and 14 days in the severity of inflammation, concentration of adenosine ([86.64 +/- 32.87] and [51.17 +/- 22.96] microg/g, P < 0.05) and expression of TNF-alpha (0.37 +/- 0.08 and 0.32 +/- 0.06, P < 0.05), but exhibited no remarkable difference in the expression of IL-10 (0.12 +/- 0.06 and 0.15 +/- 0.06, P > 0.05).
  
  
  CONCLUSIONS
  Adenosine may affect the progression of inflammation by regulating the expressions of the cytokines TNF-alpha and IL-10 in ABP rats through the adenosine receptor signaling pathway.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Long
authors.forename: Xia-Ming
authors.initials: XM
authors.lastname: Pei
authors.forename: Le-Ye
authors.initials: LY
authors.lastname: He
authors.forename: Ying-Bo
authors.initials: YB
authors.lastname: Dai
authors.forename: Dong-Yi
authors.initials: DY
authors.lastname: Peng
authors.forename: Yi-Chuan
authors.initials: YC
authors.lastname: Zhang
authors.forename: Xuan-Yan
authors.initials: XY
authors.lastname: Shi
authors.forename: Jing-Liang
authors.initials: JL
authors.lastname: He
mesh_terms: D000241:Adenosine / Q000502:physiology*; D000818:Animals; D019453:Escherichia coli O157; D016753:Interleukin-10 / Q000378:metabolism*; D008297:Male; D011467:Prostate / Q000187:drug effects / Q000378:metabolism; D011472:Prostatitis / Q000378:metabolism* / Q000382:microbiology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013806:Theophylline / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D013806:Theophylline; D000241:Adenosine