title: Antioxidant and antityrosinase proanthocyanidins from Polyalthia longifolia leaves.
abstract: In the present study the structure of proanthocyanidins from Polyalthia longifolia leaves was characterized with (13)C nuclear magnetic resonance, high performance liquid chromatography electrospray ionization mass spectrometry, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses. The results showed that the proanthocyanidins were mixture of homopolymers of B-type procyanidins with degree of polymerization up to 14-mer. Furthermore, the antioxidant activity of the proanthocyanidins was studied through 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free-radical scavenging activities, and ferric reducing/antioxidant power assays. In addition, antityrosinase activity of the proanthocyanidins was investigated. The IC50 for 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free-radical scavenging activity of the proanthocyanidins were 89.32 ± 12.07 and 76.79 ± 5.88 μg/mL, respectively; the ferric reducing/antioxidant power value was 710.54 ± 142.82 mg ascorbic acid equivalent/g dry weight. The IC50 for antityrosinase activity was 773.09 ± 1.47 μg/mL. In conclusion, the proanthocyanidins from P. longifolia leaves exhibited potent antioxidant and antityrosinase activities. This research would provide scientific evidence for the use of proanthocyanidins from P. longifolia leaves as antioxidant and antityrosinase agents.
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
authors.forename: Xiao-Xin
authors.initials: XX
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Liang
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Chai
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Feng
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
authors.forename: Han-Tao
authors.initials: HT
authors.lastname: Zhou
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China. Electronic address: chenqx@xmu.edu.cn.
authors.forename: Qing-Xi
authors.initials: QX
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D001205:Ascorbic Acid / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry; D044946:Biflavonoids / Q000737:chemistry / Q000302:isolation & purification; D001713:Biphenyl Compounds / Q000737:chemistry / Q000302:isolation & purification; D002392:Catechin / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D031044:Polyalthia / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019163:Reducing Agents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D044946:Biflavonoids; D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D010851:Picrates; D044945:Proanthocyanidins; D019163:Reducing Agents; D013451:Sulfonic Acids; C479578:procyanidin B; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D002392:Catechin; C004931:1,1-diphenyl-2-picrylhydrazyl; D014442:Monophenol Monooxygenase; D001205:Ascorbic Acid
keywords: Antioxidant activity; Antityrosinase activity; Polyalthia longifolia; Proanthocyanidins; Structure characterization