title: Optimization of selenylation conditions for a pectic polysaccharide and its structural characteristic.
abstract: CPP1b is a pectic polysaccharide isolated from Codonopsis pilosula, and it possesses potent antitumor activity. In this study, a HNO3-Na2SeO3 method was performed to synthesize selenium-CPP1b (sCPP1b). The effects of BaCl2 usage amount and HNO3 concentration on the yield and selenium content of sCPP1b were investigated by single-factor experiments. Reaction time, reaction temperature and the ratio of polysaccharide to Na2SeO3 were optimized by orthogonal experimental design at three levels of each of the three factors (L9(3)(4)) based on antitumor activity, selenium content and yield of sCPP1b. Our results showed that 5h of reaction time, 60°C of reaction temperature, and 2:2 ratio of polysaccharide to Na2SeO3 were the optimal selenylation modification conditions. The validation experiments completed under the optimal conditions gave the mean selenium content and yield of sCPP1b were 478.17μg/g (RSD=5.7%) and 595mg/g (RSD=1.6%), respectively. Selenylation modification can significantly increase the antitumor activity of CPP1b in vitro. The structural characterization of sCPP1b was further characterized by Fourier-transform infrared spectroscopy, thermogravimetric analysis, and high-performance gel permeation chromatography coupled with multi-angle laser light scattering. These structure analysis results indicated that sCPP1b has been successfully selenylation modified with similar structure to polysaccharide of CPP1b.
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, Lanzhou General Hospital of People Liberation Army, Lanzhou 730050, China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Chen
authors.affiliation: Traditional Chinese Medicine school of Gansu Province, Lanzhou 730050, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Wu
authors.affiliation: Lanzhou Military Command Center for Disease Prevention and Control, Lanzhou 730000, China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Gou
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China. Electronic address: hufd@lzu.edu.cn.
authors.forename: Fangdi
authors.initials: F
authors.lastname: Hu
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
authors.forename: Linjie
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Gao
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D029745:Codonopsis / Q000737:chemistry*; D006801:Humans; D010368:Pectins / Q000737:chemistry* / Q000494:pharmacology*; D012643:Selenium / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D010368:Pectins; D012643:Selenium
keywords: Antitumor activity; Polysaccharide from Codonopsis pilosula; Selenylation modification; Structure characteristic