title: Hairpin DNA probe-based fluorescence assay for detecting palindrome cleavage activity of HIV-1 integrase.
abstract: HIV-1 integrase is a key enzyme for retroviral replication. The integrase can perform a palindrome cleavage reaction. In this work, a hairpin DNA probe with a palindromic DNA sequence mimicking the HIV-1 LTR-LTR junction of the 2-LTR circles in its long stem was designed for fluorescence detection of specific restriction-like cleavage activity of HIV-1 integrase. Results showed that the designed probe could be recognized and cleaved by HIV-1 integrase. The palindrome cleavage reaction can be monitored according to the increase in fluorescent signal. The assay can be applied to real-time detection of palindrome cleavage of HIV-1 integrase with advantages of simplicity, high sensitivity, and specificity.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, People's Republic of China.
authors.forename: Da-wei
authors.initials: DW
authors.lastname: Zhang
authors.affiliation: Chongqing Center for Biomedicines and Medical Equipment, Chongqing Academy of Science and Technology, Chongqing, People's Republic of China.
authors.forename: Hong-qiu
authors.initials: HQ
authors.lastname: He
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, People's Republic of China. Electronic address: sxguo1986@163.com.
authors.forename: Shun-xing
authors.initials: SX
authors.lastname: Guo
mesh_terms: D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics* / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D019427:HIV Integrase / Q000378:metabolism*; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000201:enzymology* / Q000235:genetics; D055029:Inverted Repeat Sequences; D013050:Spectrometry, Fluorescence
chemical_list: D015342:DNA Probes; D019427:HIV Integrase; C514801:p31 integrase protein, Human immunodeficiency virus 1
keywords: Fluorescent probe; HIV-1; Integrase; Palindrome cleavage