title: PCB153 and p,p'-DDE disorder thyroid hormones via thyroglobulin, deiodinase 2, transthyretin, hepatic enzymes and receptors.
abstract: Polychlorinated biphenyls (PCBs) and DDT are widespread environmental persistent organic pollutants that have various adverse effects on reproduction, development and endocrine function. In order to elucidate effects of PCBs and DDT on thyroid hormone homeostasis, Sprague-Dawley rats were dosed with PCB153 and p,p'-DDE intraperitoneally (ip) for five consecutive days and sacrificed within 24 h after the last dose. Results indicated that after combined exposure to PCB153 and p,p'-DDE, total thyroxine , free thyroxine, total triiodothyronine, and thyroid-stimulating hormone in serum were decreased, whereas free triiodothyronine and thyrotropin-releasing hormone were not affected. Thyroglobulin and transthyretin levels in serum were significantly reduced. mRNA expression of deiodinases 2 (D2) was also suppressed, while D1 and D3 levels were not significantly influenced after combined exposure. PCB153 and p,p'-DDE induced hepatic enzymes, UDPGTs, CYP1A1, CYP2B1, and CYP3A1 mRNA expressions being significantly elevated. Moreover, TRα1, TRβ1, and TRHr expressions in the hypothalamus displayed increasing trends after combined exposure to PCB153 and p,p'-DDE. Taken together, observed results indicate that PCB153 and p,p'-DDE could disorder thyroid hormone homeostasis via thyroglobulin, deiodinase 2, transthyretin, hepatic enzymes, and hormone receptors.
authors.affiliation: Key Lab of Birth Defects and Reproductive Health of National Health and Family Planning Commission, Chongqing Population and family planning Science and Technology Research Institute, Chongqing, 400020, People's Republic of China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Ha
authors.forename: Lianbing
authors.initials: L
authors.lastname: Li
authors.forename: Kedi
authors.initials: K
authors.lastname: Yang
mesh_terms: D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D019362:Cytochrome P-450 CYP2B1 / Q000235:genetics; D051544:Cytochrome P-450 CYP3A / Q000235:genetics; D003633:Dichlorodiphenyl Dichloroethylene / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity*; D014453:Glucuronosyltransferase / Q000235:genetics; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007453:Iodide Peroxidase / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011228:Prealbumin / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D011988:Receptors, Thyroid Hormone / Q000378:metabolism; D013954:Thyroglobulin / Q000097:blood; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
chemical_list: D004785:Environmental Pollutants; D011228:Prealbumin; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D003633:Dichlorodiphenyl Dichloroethylene; D013972:Thyrotropin; D013954:Thyroglobulin; D011078:Polychlorinated Biphenyls; D007453:Iodide Peroxidase; C465138:Cyp3a23-3a1 protein, rat; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1; D051544:Cytochrome P-450 CYP3A; D014453:Glucuronosyltransferase; D013974:Thyroxine; C014024:2,4,5,2',4',5'-hexachlorobiphenyl