title: SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation.
abstract: Gametogenesis is a complex biological process of producing cells for sexual reproduction. Xlr super family members containing a conserved COR1 domain play essential roles in gametogenesis. In the present study, we identified that Slx2, a novel member of Xlr super family, is specifically expressed in the meiotic oocytes, which is demonstrated by western blotting and immunohistochemistry studies. In the first meiotic prophase, SLX2 is unevenly distributed in the nuclei of oocytes, during which phase SLX2 is partly co-localized with SYCP3 in synaptonemal complex and γH2AX in the nucleus of oocytes. Interestingly, the localization of SLX2 was found to be switched into the cytoplasm of oocytes after prometaphase I during oocyte maturation. Furthermore, yeast two-hybrid and coimmunoprecipitation studies demonstrated that SLX2 interacts with BLOS2, which is a novel centrosome-associated protein, and co-localized with γ-Tubulin, which is a protein marker of chromosome segregation in meiosis. These results indicated that SLX2 might get involved in chromosomes segregation during meiosis by interaction with BLOS2. In conclusion, SLX2 might be a novel gametogenesis-related protein that could play multiple roles in regulation of meiotic processes including synaptonemal complex assembly and chromosome segregation.
authors.affiliation: Center for Reproductive Medicine; Department of Obstetrics and Gynecology; Key Laboratory of Assisted Reproduction; Ministry of Education; Peking University Third Hospital; Beijing, PR China.
authors.forename: Xin-Jie
authors.initials: XJ
authors.lastname: Zhuang
authors.affiliation: College of Bio-Engineering; Weifang University; Weifang, PR China.
authors.forename: Yu-Qiang
authors.initials: YQ
authors.lastname: Shi
authors.affiliation: Center for Reproductive Medicine; Anhui Provincial Hospital Affiliated to Anhui Medical University; Hefei, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.affiliation: Reproductive Medical Centre; First Affiliated Hospital of Zhengzhou University; Zhengzhou, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Urology; the Third Hospital of Peking University; Beijing, PR China.
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Tang
authors.affiliation: Center for Reproductive Medicine; Department of Obstetrics and Gynecology; Key Laboratory of Assisted Reproduction; Ministry of Education; Peking University Third Hospital; Beijing, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.affiliation: Center for Reproductive Medicine; Department of Obstetrics and Gynecology; Key Laboratory of Assisted Reproduction; Ministry of Education; Peking University Third Hospital; Beijing, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lian
authors.affiliation: Center for Reproductive Medicine; Department of Obstetrics and Gynecology; Key Laboratory of Assisted Reproduction; Ministry of Education; Peking University Third Hospital; Beijing, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Center for Reproductive Medicine; Department of Obstetrics and Gynecology; Key Laboratory of Assisted Reproduction; Ministry of Education; Peking University Third Hospital; Beijing, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins; D020090:Chromosome Segregation; D004268:DNA-Binding Proteins; D005260:Female; D018507:Gene Expression Regulation, Developmental; D057809:HEK293 Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D008297:Male; D008540:Meiosis; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis; D011485:Protein Binding; D011506:Proteins; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013573:Synaptonemal Complex / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014162:Transfection; D014404:Tubulin
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D006657:Histones; D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger; D014404:Tubulin; C000603462:BLOS2 protein, mouse; C490062:Sycp3 protein, mouse; C000603461:synaptonemal complex protein 2, mouse; C475961:gamma-H2AX protein, mouse
keywords: BLOS2; Meiosis; Oocyte; SLX2; Spindle