title: BMI1 and TWIST1 downregulated mRNA expression in basal cell carcinoma.
abstract: BACKGROUND
  BMI1, TWIST1 and SNAI2/SLUG have been implicated in aggressive behavior of squamous cell carcinoma (SCC) and melanoma and BMI1 expression could identify subtypes of Merkel cell carcinoma (MCC). However, BMI1, TWIST1 and SNAI2 expression levels in basal cell carcinomas (BCCs) have not been elucidated. We hypothesized BCC could be a good model system to decipher mechanisms which inhibit processes that drive tumor metastasis. The aim of this study was to examine the mRNA expression level of BMI1, TWIST1, and SNAI2 in BCCs.
  
  
  METHODS
  Thirty-five fresh non-metastatic BCC tissue samples and seven fresh normal skin tissue samples were evaluated by real-time RT-PCR.
  
  
  RESULTS
  BMI1 and TWIST1 demonstrated marked down-regulation (p<0.00l, p=0.00l respectively), but SNAI2 showed no significant change (p=0.12).
  
  
  CONCLUSIONS
  Previous literature has clearly demonstrated a positive association between BMI1 and TWIST1 expression and metastatic BCC, aggressive SCC and melanoma. Here, we demonstrated a negative association between BMI1 and TWIST1 mRNA expression level and BCC.
authors.affiliation: Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran E-mail : tabrizi@tums.ac.ir.
authors.forename: Fatemeh Vand
authors.initials: FV
authors.lastname: Rajabpour
authors.forename: Reza
authors.initials: R
authors.lastname: Raoofian
authors.forename: Leila
authors.initials: L
authors.lastname: Youssefian
authors.forename: Hassan
authors.initials: H
authors.lastname: Vahidnezhad
authors.forename: Mostafa Mirshams
authors.initials: MM
authors.lastname: Shahshahani
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Fathi
authors.forename: Pedram
authors.initials: P
authors.lastname: Noormohammadpour
authors.forename: Kambiz Kamyab
authors.initials: KK
authors.lastname: Hesari
authors.forename: Morteza
authors.initials: M
authors.lastname: Hashemzadeh-Chaleshtori
authors.forename: Mina
authors.initials: M
authors.lastname: Tabrizi
mesh_terms: D000368:Aged; D002280:Basal Cell Carcinoma / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D063150:Polycomb Repressive Complex 1 / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000235:genetics*; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics*; D051879:Twist-Related Protein 1 / Q000235:genetics*
chemical_list: C069077:BMI1 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; C000604407:SNAI1 protein, human; C000604408:SNAI2 protein, human; D000071250:Snail Family Transcription Factors; C103173:TWIST1 protein, human; D014157:Transcription Factors; D051879:Twist-Related Protein 1; D063150:Polycomb Repressive Complex 1