title: Marine phytoplankton motility sensor integrated into a microfluidic chip for high-throughput pollutant toxicity assessment.
abstract: A microfluidic chip was designed to assess the toxicity of pollutants in a high-throughput way by using marine phytoplankton motility as a sensor signal. In this chip, multiple gradient generators (CGGs) with diffusible chambers enable large scale of dose-response bioassays to be performed in a simple way. Two mobile marine phytoplankton cells were confined on-chip and stimulated by 8 concentrations (generated by CGG) of Hg, Pb, Cu and phenol singly, as well as Cu and phenol jointly. CASA system was used to characterize motility by motile percentage (%MOT), curvilinear velocity (VCL), average path velocity (VAP) and straight line velocity (VSL). In all cases, dose-dependent inhibitions of motility were observed. In the present system, only 2h was needed to predict EC50. Thus, the developed microfluidic chip device was proved to be useful as a rapid/simple and high-throughput test method in marine pollution toxicity assessment.
authors.affiliation: Chemical and Environmental Engineering Institute, Dalian University, Dalian, China; Microfluidic Research Institute, Dalian University, Dalian, China. Electronic address: guoxiazheng@gmail.com.
authors.forename: Guo-xia
authors.initials: GX
authors.lastname: Zheng
authors.affiliation: Medical School, Dalian University, Dalian, China; Microfluidic Research Institute, Dalian University, Dalian, China.
authors.forename: Ya-jie
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Chemical and Environmental Engineering Institute, Dalian University, Dalian, China; Medical School, Dalian University, Dalian, China.
authors.forename: Lin-lin
authors.initials: LL
authors.lastname: Qi
authors.affiliation: Microfluidic Research Institute, Dalian University, Dalian, China; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Xian-ming
authors.initials: XM
authors.lastname: Liu
authors.affiliation: Chemical and Environmental Engineering Institute, Dalian University, Dalian, China; Microfluidic Research Institute, Dalian University, Dalian, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Wang
authors.affiliation: Chemical and Environmental Engineering Institute, Dalian University, Dalian, China; Microfluidic Research Institute, Dalian University, Dalian, China.
authors.forename: Shu-ping
authors.initials: SP
authors.lastname: Yu
authors.affiliation: Medical School, Dalian University, Dalian, China; Microfluidic Research Institute, Dalian University, Dalian, China. Electronic address: yunhua1977@126.com.
authors.forename: Yun-hua
authors.initials: YH
authors.lastname: Wang
mesh_terms: D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009068:Movement; D009792:Oceans and Seas; D010839:Phytoplankton / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical
keywords: High-throughput; Marine phytoplankton; Microfluidic chip; Motility; Toxicity assessment