title: Tumor suppressor miR-24 restrains gastric cancer progression by downregulating RegIV.
abstract: BACKGROUND
  microRNAs are small noncoding RNAs that modulate a variety of cellular processes by regulating multiple targets, which can promote or inhibit the development of malignant behaviors. Accumulating evidence suggests miR-24 plays important roles in human carcinogenesis. However, its precise biological role remains largely elusive. This study examined the role of miR-24 in gastric cancer (GC).
  
  
  METHODS
  The expression of miR-24 in GC tissues compared with matched non-tumor tissues and GC cells was detected by qRT-PCR. Synthetic short single or double stranded RNA oligonucleotides and lentiviral vectors were used to regulate miR-24 expression in GC cells to investigate its function in vitro and in vivo.
  
  
  RESULTS
  miR-24 was significantly downregulated in GC tissues compared with matched non-tumor tissues and was associated with tumor differentiation. Ectopic expression of miR-24 in SGC-7901 GC cells suppressed cell proliferation, migration and invasion in vitro as well as tumorigenicity in vivo by inducing cell cycle arrest in G0/G1 phase and promoting cell apoptosis. Furthermore, we identified RegIV as a target of miR-24 and demonstrated that miR-24 regulated RegIV expression via binding its 3' untranslated region.
  
  
  CONCLUSIONS
  miR-24 functions as a novel tumor suppressor in GC and the anti-oncogenic activity may involve its inhibition of the target gene RegIV. These findings suggest the possibility for miR-24 as a therapeutic target in GC.
authors.forename: Yantao
authors.initials: Y
authors.lastname: Duan
authors.forename: Lei
authors.initials: L
authors.lastname: Hu
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Beiqin
authors.initials: B
authors.lastname: Yu
authors.forename: Jianfang
authors.initials: J
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Yan
authors.forename: Yingyan
authors.initials: Y
authors.lastname: Yu
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Liping
authors.initials: L
authors.lastname: Su
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Zhu
authors.forename: Ming
authors.initials: M
authors.lastname: Xiang
authors.affiliation: Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No 197 Ruijin er Road, Shanghai 200025, People's Republic of China. byliu@sjtu.edu.cn.
authors.forename: Bingya
authors.initials: B
authors.lastname: Liu
authors.forename: Qiumeng
authors.initials: Q
authors.lastname: Yang
mesh_terms: D020413:3' Untranslated Regions; D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000368:Aged; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D044127:Epigenesis, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics* / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D000073718:Pancreatitis-Associated Proteins; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D020413:3' Untranslated Regions; D037181:Lectins, C-Type; C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D000073718:Pancreatitis-Associated Proteins; C428528:REG4 protein, human; D034741:RNA, Small Interfering