title: Identification of α-fodrin as an autoantigen in experimental coronavirus retinopathy (ECOR).
abstract: The coronavirus, mouse hepatitis virus (MHV), JHM strain induces a biphasic disease in BALB/c mice that consists of an acute retinitis followed by progression to a chronic retinal degeneration with autoimmune reactivity. Retinal degeneration resistant CD-1 mice do not develop either the late phase or autoimmune reactivity. A mouse RPE/choroid DNA expression library was screened using sera from virus infected BALB/c mice. Two clones were identified, villin-2 protein and α-fodrin protein. α-Fodrin protein was used for further analysis and western blot reactivity was seen only in sera from virus infected BALB/c mice. CD4 T cells were shown to specifically react with MHV antigens and with α-fodrin protein. These studies clearly identified both antibody and CD4 T cell reactivities to α-fodrin in sera from virus infected, retinal degenerative susceptible BALB/c mice.
authors.affiliation: Immunology and Virology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD, United States.
authors.forename: Marian S
authors.initials: MS
authors.lastname: Chin
authors.affiliation: Immunology and Virology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD, United States.
authors.forename: Laura C
authors.initials: LC
authors.lastname: Hooper
authors.affiliation: Immunology and Virology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD, United States.
authors.forename: John J
authors.initials: JJ
authors.lastname: Hooks
authors.affiliation: Department of Pathology, Johns Hopkins University, School of Medicine, Baltimore, MD, United States.
authors.forename: Barbara
authors.initials: B
authors.lastname: Detrick
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000276:immunology*; D015704:CD4 Antigens / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D017934:Coronavirus / Q000472:pathogenicity; D018352:Coronavirus Infections / Q000150:complications*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D015723:Gene Library; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012160:Retina / Q000378:metabolism / Q000473:pathology; D012162:Retinal Degeneration / Q000209:etiology* / Q000473:pathology / Q000821:virology*; D017422:Sequence Analysis, DNA; D013997:Time Factors; D013049:Spectrin; D000099149:Ezrin
chemical_list: D001324:Autoantigens; D015704:CD4 Antigens; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D010835:Phytohemagglutinins; D013049:Spectrin; D000099149:Ezrin
keywords: Autoantibodies; Autoimmunity; Coronavirus; Retinal degeneration; α-Fodrin