title: Fast functionalization of silver decahedral nanoparticles with aptamers for colorimetric detection of human platelet-derived growth factor-BB.
abstract: Aptamer-silver decahedral nanoparticles (Ag10NPs-aptamer) based detection was developed for protein. Ag10NPs were synthesized by photochemical method. The advantage of Ag10NPs was its tolerance of NaCl which facilitates the functionalization of silver nanoparticles with all kinds of ssDNA. Attaching aptamers to Ag10NPs could be achieved within 2 h, much faster than traditional methods. Human platelet-derived growth factor-BB (PDGF-BB) was used as a model protein to test the binding capacity of aptamers attached on Ag10NPs. Our data showed that the aptamer-Ag10NPs conjugates were successful in detecting human PDGF-BB. Furthermore, we developed an aptamer-Ag10NPs conjugates-based colorimetric sensor to detect PDGF-BB. The results showed a linear relationship between PDGF-BB concentrations (5 ng mL(-1)-200 ng mL(-1)) and ΔOD with excellent detection specificity in serum. Therefore, the sensor based on aptamer-Ag10NPs conjugates was highly effective and sensitive and had great promise for further development and applications.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, China. Electronic address: lihui@nju.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, China. Electronic address: 237573395@qq.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, China. Electronic address: 18947180075@126.com.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, China. Electronic address: qiangwb1987@126.com.
authors.forename: Weibing
authors.initials: W
authors.lastname: Qiang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, China. Electronic address: sunliang@nju.edu.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, China. Electronic address: xudanke@nju.edu.cn.
authors.forename: Danke
authors.initials: D
authors.lastname: Xu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D000077214:Becaplermin; D003124:Colorimetry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis*; D012834:Silver / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D012834:Silver
keywords: Aptamer; Platelet-derived growth factor-BB; Serum; Silver decahedral nanoparticles