title: Single-molecule dynamics of site-specific labeled transforming growth factor type II receptors on living cells.
abstract: We achieved single-molecule imaging and tracking of the transforming growth factor type II receptor (TβRII) that was labeled by an organic dye via a genetically encoded unnatural amino acid (UAA) and the copper-free click chemistry. The stoichiometry, mobility and dimerization kinetics of individual TβRII molecules were determined.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Key Laboratory of Molecular Nanostructures and Nanotechnology, Chinese Academy of Sciences, Beijing 100190, P. R. China. xfang@iccas.ac.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Cheng
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jinghe
authors.initials: J
authors.lastname: Yuan
authors.forename: Wangxi
authors.initials: W
authors.lastname: Luo
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Shixian
authors.initials: S
authors.lastname: Lin
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Fang
authors.forename: Peng R
authors.initials: PR
authors.lastname: Chen
mesh_terms: D001386:Azides / Q000737:chemistry; D001665:Binding Sites; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D057054:Molecular Imaging; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D001386:Azides; D005456:Fluorescent Dyes; D018125:Receptors, Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II