title: The wheat aquaporin gene TaAQP7 confers tolerance to cold stress in transgenic tobacco.
abstract: Aquaporin proteins (AQPs) have been shown to be involved in abiotic stress responses. However, the precise role of AQPs, especially in response to cold stress, is not understood in wheat (Triticum aestivum). In the present study, quantitative real time polymerase chain reaction (qRT-PCR) analysis revealed that TaAQP7 expression increased in leaves, but decreased in roots after cold treatment. Expression of TaAQP7 in tobacco plants resulted in increased root elongation and better growth compared with wild-type (WT) plants under cold stress. Moreover, after cold treatment, the transgenic tobacco lines exhibited higher chlorophyll contents, lower levels of malondialdehyde (MDA), and less ion leakage (IL) than WT plants. Thus, expression of TaAQP7 enhanced cold stress tolerance in transgenic tobacco. Taken together, our results suggest that TaAQP7 confers cold stress tolerance by relieving membrane damage in the transgenic plants.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.forename: Shiyi
authors.initials: S
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Deng
authors.forename: Shuya
authors.initials: S
authors.lastname: Wei
authors.forename: Guangxiao
authors.initials: G
authors.lastname: Yang
authors.forename: Guangyuan
authors.initials: G
authors.lastname: He
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D020346:Aquaporins / Q000235:genetics*; D003080:Cold Temperature; D017343:Genes, Plant; D008315:Malondialdehyde / Q000378:metabolism; D030821:Plants, Genetically Modified; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014908:Triticum / Q000235:genetics*
chemical_list: D020346:Aquaporins; D008315:Malondialdehyde