title: Enhancing trichloroethylene degradation using non-aromatic compounds as growth substrates.
abstract: The effect of non-aromatic compounds on the trichloroethylene (TCE) degradation of toluene-oxidizing bacteria were evaluated using Burkholderia cepacia G4 that expresses toluene 2-monooxygenase and Pseudomonas putida that expresses toluene dioxygenase. TCE degradation rates for B. cepacia G4 and P. putida with toluene alone as growth substrate were 0.144 and 0.123 μg-TCE/mg-protein h, respectively. When glucose, acetate and ethanol were fed as additional growth substrates, those values increased up to 0.196, 0.418 and 0.530 μg-TCE/mg-protein h, respectively for B. cepacia G4 and 0.319, 0.219 and 0.373 μg-TCE/mg-protein h, respectively for P. putida. In particular, the addition of ethanol resulted in a high TCE degradation rate regardless of the initial concentration. The use of a non-aromatic compound as an additional substrate probably enhanced the TCE degradation because of the additional supply of NADH that is consumed in co-metabolic degradation of TCE. Also, it is expected that the addition of a non-aromatic substrate can reduce the necessary dose of toluene and, subsequently, minimize the potential competitive inhibition upon TCE co-metabolism by toluene.
authors.affiliation: Department of Civil Environmental Engineering, Hanyang University, Sa-Dong, Ansan, Gyeonggi-Do, 425-791, Republic of Korea.
authors.forename: Seungjin
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Civil Environmental Engineering, Hanyang University, Sa-Dong, Ansan, Gyeonggi-Do, 425-791, Republic of Korea.
authors.forename: Jeongmin
authors.initials: J
authors.lastname: Hwang
authors.affiliation: R&D Center, Samsung Engineering Co. Ltd., Woncheon-Dong, Youngtong-Gu, Suwon, Gyeonggi-Do, 443-823, Republic of Korea.
authors.forename: Jinwook
authors.initials: J
authors.lastname: Chung
authors.affiliation: Department of Civil Environmental Engineering, Hanyang University, Sa-Dong, Ansan, Gyeonggi-Do, 425-791, Republic of Korea. Electronic address: wkbae@hanyang.ac.kr.
authors.forename: Wookeun
authors.initials: W
authors.lastname: Bae
mesh_terms: D000085:Acetates / Q000494:pharmacology; D001673:Biodegradation, Environmental; D016956:Burkholderia cepacia / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D016958:Pseudomonas putida / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014241:Trichloroethylene / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D000085:Acetates; D014874:Water Pollutants, Chemical; D014241:Trichloroethylene; D000431:Ethanol; D005947:Glucose
keywords: Burkholderia cepacia G4; Cometabolism; Pseudomonas putida; Toluene; Trichloroethylene