title: Development and evaluation of liquid embolic agents based on liquid crystalline material of glyceryl monooleate.
abstract: New type of liquid embolic agents based on a liquid crystalline material of glyceryl monooleate (GMO) was developed and evaluated in this study. Ternary phase diagram of GMO, water and ethanol was constructed and three isotropic liquids (ILs, GMO:ethanol:water=49:21:30, 60:20:20 and 72:18:10 (w/w/w)) were selected as potential liquid embolic agents, which could spontaneously form viscous gel cast when contacting with water or physiological fluid. The ILs exhibited excellent microcatheter deliverability due to low viscosity, and were proved to successfully block the saline flow when performed in a device to simulate embolization in vitro. The ILs also showed good cytocompatibility on L929 mouse fibroblast cell line. The embolization of ILs to rabbit kidneys was performed successfully under monitoring of digital subtraction angiography (DSA), and embolic degree was affected by the initial formulation composition and used volume. At 5th week after embolization, DSA and computed tomography (CT) confirmed the renal arteries embolized with IL did not recanalize in follow-up period, and an obvious atrophy of the embolized kidney was observed. Therefore, the GMO-based liquid embolic agents showed feasible and effective to embolize, and potential use in clinical interventional embolization therapy.
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors.forename: Ling-Ran
authors.initials: LR
authors.lastname: Du
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Lu
authors.affiliation: Department of Interventional Radiology and Vascular Surgery, Peking University First Hospital, Beijing 100034, China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Guan
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors.forename: Yong-Jie
authors.initials: YJ
authors.lastname: Yang
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Gu
authors.affiliation: Department of Radiology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Zhuo-Zhao
authors.initials: ZZ
authors.lastname: Zheng
authors.affiliation: Department of Interventional Radiology and Vascular Surgery, Peking University First Hospital, Beijing 100034, China.
authors.forename: Tian-Shi
authors.initials: TS
authors.lastname: Lv
authors.affiliation: Department of Interventional Radiology and Vascular Surgery, Peking University First Hospital, Beijing 100034, China.
authors.forename: Zi-Guang
authors.initials: ZG
authors.lastname: Yan
authors.affiliation: Department of Interventional Radiology and Vascular Surgery, Peking University First Hospital, Beijing 100034, China.
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Interventional Radiology and Vascular Surgery, Peking University First Hospital, Beijing 100034, China.
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Zou
authors.affiliation: Department of Radiology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Nai-Qi
authors.initials: NQ
authors.lastname: Fu
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors.forename: Xian-Rong
authors.initials: XR
authors.lastname: Qi
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: tianyuan_fan@bjmu.edu.cn.
authors.forename: Tian-Yuan
authors.initials: TY
authors.lastname: Fan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004621:Embolization, Therapeutic / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects; D005782:Gels; D005989:Glycerides / Q000737:chemistry* / Q000633:toxicity; D050866:Liquid Crystals / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D044367:Phase Transition; D011817:Rabbits; D011859:Radiography; D012077:Renal Artery / Q000000981:diagnostic imaging / Q000187:drug effects; D014783:Viscosity; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D005989:Glycerides; D014867:Water; D000431:Ethanol; C005953:monoolein
keywords: Embolization; Glyceryl monooleate; Isotropic liquid; Liquid crystalline material; Liquid embolic agent