title: Trifluoroethanethiol: an additive for efficient one-pot peptide ligation-desulfurization chemistry.
abstract: Native chemical ligation followed by desulfurization is a powerful strategy for the assembly of proteins. Here we describe the development of a high-yielding, one-pot ligation-desulfurization protocol that uses trifluoroethanethiol (TFET) as a novel thiol additive. The synthetic utility of this TFET-enabled methodology is demonstrated by the efficient multi-step one-pot syntheses of two tick-derived proteins, chimadanin and madanin-1, without the need for any intermediary purification.
authors.affiliation: School of Chemistry, The University of Sydney , Sydney, NSW 2006, Australia.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Thompson
authors.forename: Xuyu
authors.initials: X
authors.lastname: Liu
authors.forename: Noelia
authors.initials: N
authors.lastname: Alonso-García
authors.forename: Pedro José Barbosa
authors.initials: PJ
authors.lastname: Pereira
authors.forename: Katrina A
authors.initials: KA
authors.lastname: Jolliffe
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Payne
mesh_terms: D060326:Chemistry Techniques, Synthetic; D002851:Chromatography, High Pressure Liquid; D019476:Insect Proteins / Q000138:chemical synthesis* / Q000737:chemistry*; D013058:Mass Spectrometry; D014270:Trifluoroethanol / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: C000597631:2,2,2-trifluoroethanethiol; D019476:Insect Proteins; C517571:chimadanin; D014270:Trifluoroethanol