title: Metabolite profiling and fingerprinting of Suillus species (Basidiomycetes) by electrospray mass spectrometry.
abstract: The genus Suillus is known for the occurrence of a series of prenylated phenols and boviquinones. The extracts of four different Suillus species [S. bovinus, S. granulatus, S. tridentinus and S.variegatus) were investigated by using rapid ultra-performance Liquid chromatography/electrospray ionization mass spectrometry (UPLC/ESI-MS) and direct infusion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS). While direct infusion ESI-FT-ICR mass spectra give a fast overview concerning the elemental compositions of the compounds and, therefore, hints to the main metabolites, UPLC/ESI-tandem mass spectrometry is shown to be a useful tool for their identification. A principal component analysis (PCA) and hierarchical cluster analysis (HCA) based on the UPLC/ESI-MS clearly showed that the metabolite profiles can be used not only for the identification and classification of such fungi but also as a sophisticated and powerful tool for the chemotaxonomy of fungi. Furthermore, a clear discrimination of various types of biological samples (fruiting bodies versus mycelial cultures) is also possible. The orthogonal partial least squares (OPLS) two-class models of both UPLC/ESI-MS and ESI-FT-ICR-MS possess a clear differentiation of two compared Suillus species representing the between class variation and the within class variation. Based on generated S-plots and Loading plots, statistically significant metabolites could be identified as potential biomarker for one species.
authors.forename: Ramona
authors.initials: R
authors.lastname: Heinke
authors.forename: Pia
authors.initials: P
authors.lastname: Schöne
authors.forename: Norbert
authors.initials: N
authors.lastname: Arnold
authors.forename: Ludger
authors.initials: L
authors.lastname: Wessjohann
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schmidt
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schmidt
mesh_terms: D001487:Basidiomycota / Q000737:chemistry* / Q000145:classification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005583:Fourier Analysis; D055442:Metabolome; D010636:Phenols / Q000032:analysis; D011809:Quinones / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010636:Phenols; D011809:Quinones