title: Zinc and iron concentration QTL mapped in a Triticum spelta × T. aestivum cross.
abstract: Ten QTL underlying the accumulation of Zn and Fe in the grain were mapped in a set of RILs bred from the cross Triticum spelta × T. aestivum . Five of these loci (two for Zn and three for Fe) were consistently detected across seven environments. The genetic basis of accumulation in the grain of Zn and Fe was investigated via QTL mapping in a recombinant inbred line (RIL) population bred from a cross between Triticum spelta and T. aestivum. The concentration of the two elements was measured from grain produced in three locations over two consecutive cropping seasons and from a greenhouse trial. The range in Zn and Fe concentration across the RILs was, respectively, 18.8-73.5 and 25.3-59.5 ppm, and the concentrations of the two elements were positively correlated with one another (rp =+0.79). Ten QTL (five each for Zn and Fe accumulation) were detected, mapping to seven different chromosomes. The chromosome 2B and 6A grain Zn QTL were consistently expressed across environments. The proportion of the phenotype explained (PVE) by QZn.bhu-2B was >16 %, and the locus was closely linked to the SNP marker 1101425|F|0, while QZn.bhu-6A (7.0 % PVE) was closely linked to DArT marker 3026160|F|0. Of the five Fe QTL detected, three, all mapping to chromosome 1A were detected in all seven environments. The PVE for QFe.bhu-3B was 26.0 %.
authors.affiliation: Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.
authors.forename: Jayasudha
authors.initials: J
authors.lastname: Srinivasa
authors.forename: Balasubramaniam
authors.initials: B
authors.lastname: Arun
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Mishra
authors.forename: Gyanendra Pratap
authors.initials: GP
authors.lastname: Singh
authors.forename: Govindan
authors.initials: G
authors.lastname: Velu
authors.forename: Raman
authors.initials: R
authors.lastname: Babu
authors.forename: Neeraj Kumar
authors.initials: NK
authors.lastname: Vasistha
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Joshi
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D008040:Genetic Linkage; D014644:Genetic Variation; D007501:Iron / Q000032:analysis*; D010641:Phenotype; D040641:Quantitative Trait Loci; D014908:Triticum / Q000737:chemistry / Q000235:genetics*; D015032:Zinc / Q000032:analysis*
chemical_list: D018744:DNA, Plant; D007501:Iron; D015032:Zinc