title: Crystal structure of the C-terminal domain of mouse TLR9.
abstract: Toll-like receptors (TLRs) are important pattern recognition receptors that function in innate immunity. Elucidating the structure and signaling mechanisms of TLR9, a sensor of foreign and endogenous DNA, is essential for understanding its key role in immunity against microbial pathogens as well as in autoimmunity. Abundant evidence suggests that the TLR9-CTD (C-terminal domain) by itself is capable of DNA binding and signaling. The crystal structure of unliganded mouse TLR9-CTD is presented. TLR9-CTD exhibits one unique feature, a cluster of stacked aromatic and arginine side chains on its concave face. Overall, its structure is most related to the TLR8-CTD, suggesting a similar mode of ligand binding and signaling.
authors.affiliation: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California.
authors.forename: Bernard
authors.initials: B
authors.lastname: Collins
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray; D030562:Databases, Protein; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D051196:Toll-Like Receptor 3 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051200:Toll-Like Receptor 8 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C495344:TLR3 protein, human; C495349:TLR8 protein, human; C495370:Tlr9 protein, mouse; D051196:Toll-Like Receptor 3; D051200:Toll-Like Receptor 8; D051217:Toll-Like Receptor 9
keywords: DNA; LRR; TLR; TLR9; Toll-like receptor; innate immunity; leucine-rich repeat; nucleic acid