title: Postacute effects of kisspeptin-10 on neuronal injury induced by L-methionine in rats.
abstract: Apart from its effect on the regulation of reproductive function, recent studies indicate that kisspeptin may play roles in the antioxidant defense system. The antioxidant defense system and oxidative stress contribute to the etiology and pathogenesis of neuronal cell death after brain injury. We have investigated the postacute effect of kisspeptin-10 on brain injury induced by L-methionine. DNA fragmentation, malondialdehyde (MDA), reduced glutathione levels, and superoxide dismutase (SOD) activities were analyzed. Our results showed that methionine treatment increases apoptotic cell death. Kisspeptin alone showed no side effect on apoptotic cell death. However, kisspeptin treatment reversed the proapoptotic effect of methionine associated with reduced MDA and increased glutathione levels. Furthermore, SOD activity was completely depleted in methionine-treated animals. In conclusion, our results revealed that delayed kisspeptin-10 treatment reduces neuronal cell death by activation of SOD activity.
authors.affiliation: Experimental Research Center (YUDETAM), Faculty of Medicine, Yeditepe University, Istanbul, Turkey. hatice.akkaya@yeditepe.edu.tr.
authors.forename: Hatice
authors.initials: H
authors.lastname: Akkaya
authors.forename: Ertugrul
authors.initials: E
authors.lastname: Kilic
authors.forename: Signem Eyuboglu
authors.initials: SE
authors.lastname: Dinc
authors.forename: Bayram
authors.initials: B
authors.lastname: Yilmaz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D001930:Brain Injuries / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D020138:Hyperhomocysteinemia / Q000139:chemically induced / Q000188:drug therapy*; D059648:Kisspeptins / Q000008:administration & dosage* / Q000378:metabolism; D008715:Methionine / Q000633:toxicity; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; C491406:Kiss1 protein, rat; D059648:Kisspeptins; D008715:Methionine; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Apoptosis; Hyperhomocysteinemia; Kisspeptin; Methionine; Oxidative Stress