title: Antiviral activity of 3,4'-dihydroxyflavone on influenza a virus.
abstract: Influenza virus infection causes thousands of deaths and millions of hospitalizations worldwide every year and the emergence of resistance to anti-influenza drugs has prompted scientists to seek new natural antiviral materials. In this study, we screened 13 different flavonoids from various flavonoid groups to identify the most potent antiviral flavonoid against human influenza A/PR/8/34 (H1N1). The 3-hydroxyl group flavonoids, including 3,2᾿dihydroxyflavone (3,2᾿DHF) and 3,4᾿dihydroxyflavone (3,4᾿DHF), showed potent anti-influenza activity. They inhibited viral neuraminidase activity and viral adsorption onto cells. To confirm the anti-influenza activity of these flavonoids, we used an in vivo mouse model. In mice infected with human influenza, oral administration of 3,4᾿DHF significantly decreased virus titers and pathological changes in the lung and reduced body weight loss and death. Our data suggest that 3-hydroxyl group flavonoids, particularly 3,4᾿DHF, have potent antiviral activity against human influenza A/PR/8/34 (H1N1) in vitro and in vivo. Further clinical studies are needed to investigate the therapeutic and prophylactic potential of the 3-hydroxyl group flavonoids in treating influenza pandemics.
authors.affiliation: Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal model and Stem cell Institute (IDASI), Konkuk University, Seoul, 143-701, Republic of Korea.
authors.forename: Mohammed Kawser
authors.initials: MK
authors.lastname: Hossain
authors.forename: Hye Yeon
authors.initials: HY
authors.lastname: Choi
authors.forename: Jae-Seon
authors.initials: JS
authors.lastname: Hwang
authors.forename: Ahmed Abdal
authors.initials: AA
authors.lastname: Dayem
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Young Bong
authors.initials: YB
authors.lastname: Kim
authors.forename: Haryoung
authors.initials: H
authors.lastname: Poo
authors.forename: Ssang-Goo
authors.initials: SG
authors.lastname: Cho
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004285:Dogs; D047309:Flavones / Q000494:pharmacology*; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C559991:3,4'-dihydroxyflavone; D000998:Antiviral Agents; D047309:Flavones