title: Potent acetylcholinesterase inhibitory compounds from Myristica fragrans.
abstract: The anti-cholinesterase activity was evaluated of the ethyl acetate fraction of the methanol extract of Myristica fragrans Houtt (Myristicaceae) seeds and of compounds isolated from it by various chromatographic techniques. The chemical structures of the compounds were determined from spectroscopic analyses (NMR data). Thirteen compounds (1-13) were isolated and identified. Compound 8 { [(7S)-8'-(4'-hydroxy-3'-methoxyphenyl)-7-hydroxypropyl]benzene-2,4-diol) showed the most effective activity with an IC50 value of 35.1 microM, followed by compounds 2 [(8R,8'S)-7'-(3',4'-methylenedioxyphenyl)-8,8'-dimethyl-7-(3,4-dihydroxyphenyl)-butane] and 11 (malabaricone C) with IC50 values of 42.1 and 44.0 pM, respectively. This is the first report of significant anticholinesterase properties of M. fragrans seeds. The findings demonstrate that M. fragrans could be used beneficially in the treatment of Alzheimer's disease.
authors.forename: To Dao
authors.initials: TD
authors.lastname: Cuong
authors.forename: Tran Manh
authors.initials: TM
authors.lastname: Hung
authors.forename: Hyoung Yun
authors.initials: HY
authors.lastname: Han
authors.forename: Hang Sik
authors.initials: HS
authors.lastname: Roh
authors.forename: Ji-Hyeon
authors.initials: JH
authors.lastname: Seok
authors.forename: Jong Kwon
authors.initials: JK
authors.lastname: Lee
authors.forename: Ja Young
authors.initials: JY
authors.lastname: Jeong
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D015394:Molecular Structure; D026323:Myristica / Q000737:chemistry*
chemical_list: D002800:Cholinesterase Inhibitors; D000110:Acetylcholinesterase