title: Effect of EDTA, sodium hypochlorite, and chlorhexidine gluconate with or without surface modifiers on dentin microhardness.
abstract: BACKGROUND
  This study aimed to evaluate the effects of root canal irrigants on the microhardness of root canal dentin in the presence and absence of surface-modifying agents.
  
  
  METHODS
  Forty-eight root halves were prepared by longitudinal splitting of the distal roots of 24 freshly extracted mandibular human third molars and embedded in autopolymerizing acrylic resin, leaving the dentin surface exposed. After polishing, the microhardness values of the untreated dentin surfaces were recorded by using Vickers tester at the mid-root level. The root halves were randomly assigned to 6 groups composed of 8 samples each and treated for 5 minutes with one of the following irrigants: 17% EDTA, REDTA, 2% chlorhexidine gluconate (CHX), 2% CHX with surface modifiers (CHX-Plus), 6% NaOCl, or 6% NaOCl with surface modifiers (Chlor-XTRA). After surface treatment, dentin microhardness values were recorded at close proximity to the initial indentation areas. Experimental data were statistically analyzed by using the t test and one-way analysis of variance, followed by Tukey honestly significant difference test at α = 0.05.
  
  
  RESULTS
  EDTA, REDTA, NaOCl, and Chlor-XTRA significantly decreased the microhardness of root dentin compared with intact controls (P < .05).
  
  
  CONCLUSIONS
  The addition of surface modifiers to the irrigants did not affect the microhardness of the samples.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.
authors.forename: Eda E
authors.initials: EE
authors.lastname: Aslantas
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey. Electronic address: hdogan67@hotmail.com.
authors.forename: Hatice Dogan
authors.initials: HD
authors.lastname: Buzoglu
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Altundasar
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Serper
mesh_terms: D000328:Adult; D002593:Cetrimonium Compounds / Q000494:pharmacology; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003804:Dentin / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology*; D006244:Hardness; D006801:Humans; D011897:Random Allocation; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*; D013997:Time Factors; D055815:Young Adult
chemical_list: D002593:Cetrimonium Compounds; D012388:Root Canal Irrigants; D013501:Surface-Active Agents; C031883:REDTA; D004492:Edetic Acid; D012973:Sodium Hypochlorite; C010882:chlorhexidine gluconate; D002710:Chlorhexidine
keywords: Chlorhexidine gluconate; EDTA; dentin; microhardness; sodium hypochlorite; surface modifier