title: TAF7L modulates brown adipose tissue formation.
abstract: Brown adipose tissue (BAT) plays an essential role in metabolic homeostasis by dissipating energy via thermogenesis through uncoupling protein 1 (UCP1). Previously, we reported that the TATA-binding protein associated factor 7L (TAF7L) is an important regulator of white adipose tissue (WAT) differentiation. In this study, we show that TAF7L also serves as a molecular switch between brown fat and muscle lineages in vivo and in vitro. In adipose tissue, TAF7L-containing TFIID complexes associate with PPARγ to mediate DNA looping between distal enhancers and core promoter elements. Our findings suggest that the presence of the tissue-specific TAF7L subunit in TFIID functions to promote long-range chromatin interactions during BAT lineage specification.
authors.affiliation: Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States.
authors.forename: Haiying
authors.initials: H
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wan
authors.affiliation: Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States.
authors.forename: Ivan
authors.initials: I
authors.lastname: Grubisic
authors.affiliation: School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Tommy
authors.initials: T
authors.lastname: Kaplan
authors.affiliation: Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States.
authors.forename: Robert
authors.initials: R
authors.lastname: Tjian
mesh_terms: D017667:Adipocytes / Q000166:cytology; D002001:Adipose Tissue, Brown / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002843:Chromatin / Q000737:chemistry; D002875:Chromosomes / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D009132:Muscles / Q000502:physiology*; D047495:PPAR gamma / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D035381:TATA-Binding Protein Associated Factors / Q000378:metabolism*; D022722:Thermogenesis; D035362:Transcription Factor TFIID / Q000378:metabolism*
chemical_list: D002843:Chromatin; D047495:PPAR gamma; C000598119:TAF7L protein, mouse; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D049452:Green Fluorescent Proteins; D004247:DNA
keywords: BAT; DNA looping; PPARγ; UCP1; developmental biology; mouse; obesity; stem cells