title: Genotyping of Yersinia enterocolitica biotype 1A strains from clinical and nonclinical origins by pulsed-field gel electrophoresis.
abstract: Yersinia enterocolitica biotype 1A (B1A) strains are considered mainly nonpathogenic. However, some studies considered strains of this biotype to be the causal agents of infections in humans and animals. In South America, there are no studies that have compared clinical and nonclinical strains of B1A typed by pulsed-field gel electrophoresis (PFGE) and none that have compared the capability of different enzymes on typing these strains. This study typed 51 Y. enterocolitica B1A strains isolated in Brazil and Chile by PFGE, testing the enzymes XbaI, NotI, and XhoI. The resulting dendrograms discriminated the strains in 47, 40, and 49 pulsotypes generated by the cleavage with the enzymes XbaI, NotI, and XhoI, respectively. The majority of the strains were grouped independently of their clinical or nonclinical origins. The high discriminatory power of PFGE confirmed the heterogeneity of B1A strains but could not divide the strains studied into clusters that differed in the frequency of some virulence genes as observed in studies using other methodologies.
authors.affiliation: Brazilian Reference Center on Yersinia spp. other than Y. pestis, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto - USP, Avenida do Café, s/n, 14040-903, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Fábio
authors.initials: F
authors.lastname: Campioni
authors.forename: Juliana P
authors.initials: JP
authors.lastname: Falcão
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods; D001938:Brazil; D002677:Chile; D016000:Cluster Analysis; D004262:DNA Restriction Enzymes / Q000145:classification* / Q000592:standards; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods / Q000592:standards; D005838:Genotype; D060005:Genotyping Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D014774:Virulence / Q000235:genetics; D015009:Yersinia Infections / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D004262:DNA Restriction Enzymes
keywords: PFGE; Yersinia enterocolitica; Yersinia enterocolitica, biotype 1A, PFGE; biotype 1A; genotyping; génotypage