title: Pathways of reductive degradation of crystal violet in wastewater using free-strain Burkholderia vietnamiensis C09V.
abstract: A new strain isolated from activated sludge and identified as Burkholderia vietnamiensis C09V was used to biodegrade crystal violet (CV) from aqueous solution. To understand the degradation pathways of CV, batch experiments showed that the degradation using B. vietnamiensis C09V significantly depended on conditions such as pH, initial dye concentration and media components, carbon and nitrogen sources. Acceleration in the biodegradation of CV was observed in presence of metal ions such as Cd and Mn. More than 98.86C of CV (30 mg l(-1)) was degraded within 42 h at pH 5 and 30 °C. The biodegradation kinetics of CV corresponded to the pseudo first-order rate model with a rate constant of 0.046 h(-1). UV-visible and Fourier transform infrared spectroscopy (FTIR) were used to identify degradation metabolites. Which further confirmed by LC-MS analysis, indicating that CV was biodegraded to N,N-dimethylaminophenol and Michler's ketone prior to these intermediates being further degraded. Finally, the ability of B. vietnamiensis C09V to remove CV in wastewater was demonstrated.
authors.affiliation: School of Environmental Science and Engineering, Fujian Normal University, Fuzhou, 350007, Fujian Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gan
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.forename: Thavamani
authors.initials: T
authors.lastname: Palanisami
authors.forename: Zuliang
authors.initials: Z
authors.lastname: Chen
authors.forename: Mallavarapu
authors.initials: M
authors.lastname: Megharaj
authors.forename: Ravendra
authors.initials: R
authors.lastname: Naidu
mesh_terms: D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques; D005840:Gentian Violet / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013058:Mass Spectrometry; D019216:Metals, Heavy; D009584:Nitrogen / Q000378:metabolism; D012722:Sewage / Q000382:microbiology; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013783:Textile Industry; D014865:Waste Disposal, Fluid; D062065:Wastewater
chemical_list: D002244:Carbon; D005840:Gentian Violet; D019216:Metals, Heavy; D009584:Nitrogen; D012722:Sewage; D062065:Wastewater