title: Immobilized lipase from Candida sp. 99-125 on hydrophobic silicate: characterization and applications.
abstract: Lipase Candida sp. 99-125 has been proved to be quite effective in catalyzing organic synthesis reactions and is much cheaper than commercial lipases. Mesoporous silicates are attractive materials for the immobilization of enzymes due to their unique structures. The present research designed a hydrophobic silicate with uniform pore size suitable for the comfort of lipase Candida sp. 99-125 for improving its activity and stability. The resulting immobilized lipase (LP@PMO) by adsorption was employed to catalyze hydrolysis, esterification, and transesterification reactions, and the performances were compared with the lipase immobilized on hydrophilic silicate (LP@PMS) and native lipase. The LP@PMO showed as high activity as that of native lipase in hydrolysis and much increased catalytic activity and reusability in the reactions for biodiesel production. Besides, LP@PMO also possessed better organic stability. Such results demonstrate that immobilization of lipase onto hydrophobic supports is a promising strategy to fabricate highly active and stable biocatalysts for applications.
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Xinlong
authors.initials: X
authors.lastname: Liu
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Jiang
authors.forename: Liya
authors.initials: L
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
mesh_terms: D002175:Candida / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D004951:Esterification; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D017640:Silicates / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D017640:Silicates; D012997:Solvents; D008049:Lipase