title: Inflammation-induced miR-802 promotes cell proliferation in cholesteatoma.
abstract: Cholesteatoma is a destructive and abnormal skin growth consisting of keratinizing squamous epithelium in the middle ear. Its molecular mechanisms remain poorly understood. Here, we found that the NF-κB inflammatory signaling pathway was highly activated in cholesteatoma. NF-κB activation increased the expression of microRNA-802 (miR-802) and chromatin immunoprecipitation assays showed that P65 could uniquely bind to miR-802 promoter. miR-802 overexpression promoted keratinocyte cell proliferation and cell cycle progression, while inhibition of miR-802 decreased these effects. From computational analysis and luciferase report assays, miR-802 directly repressed PTEN expression by targeting its 3'-UTR. Our results demonstrate that the NF-κb/miR-802/PTEN signaling pathway plays an important role in the development of cholesteatoma.
authors.affiliation: Department of Otology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Zhao-Bing
authors.initials: ZB
authors.lastname: Qin
mesh_terms: D049109:Cell Proliferation; D002781:Cholesteatoma / Q000473:pathology*; D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation; D007249:Inflammation / Q000473:pathology*; D015603:Keratinocytes / Q000502:physiology*; D035683:MicroRNAs / Q000096:biosynthesis*; D016328:NF-kappa B / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D011485:Protein Binding; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: C552280:MIRN802 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D051996:Transcription Factor RelA; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human