title: Common variants of cGKII/PRKG2 are not associated with gout susceptibility.
abstract: OBJECTIVE
  Recently, genetic analyses indicated the association between gout and cGMP-dependent protein kinase 2 (cGKII/PRKG2) gene in a Fukien-Taiwanese heritage population. However, no replication study has been reported in other ancestries. Therefore, we investigated this association in a Japanese population.
  
  
  METHODS
  Genotyping of 4 variants (rs11736177, rs10033237, rs7688672, and rs6837293) of cGKII was performed in 741 male gout patients and 1302 male controls.
  
  
  RESULTS
  cGKII variants have no association with gout.
  
  
  CONCLUSIONS
  Our replication study suggests that cGKII is not involved in gout susceptibility.
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Sakiyama
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College. hmatsuo@ndmc.ac.jp.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Matsuo
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Chiba
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Nakayama
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Seiko
authors.initials: S
authors.lastname: Shimizu
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Emi
authors.initials: E
authors.lastname: Morita
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Nana
authors.initials: N
authors.lastname: Fukuda
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakashima
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sakurai
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Kimiyoshi
authors.initials: K
authors.lastname: Ichida
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Toru
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: From the Department of Integrative Physiology and Bio-Nano Medicine, Department of Dermatology, Laboratory for Mathematics, and Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa; Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya; Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, Tokyo; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo; and Midorigaoka Hospital, Takatsuki, Japan.M. Sakiyama, MD, Department of Integrative Physiology and Bio-Nano Medicine, and Department of Dermatology, National Defense Medical College; H. Matsuo, MD, PhD; T. Chiba, MD; A. Nakayama, MD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; T. Nakamura, PhD, Laboratory for Mathematics, National Defense Medical College; S. Shimizu, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College; E. Morita, MD, PhD; N. Fukuda, BHE, Department of Preventive Medicine, Nagoya University Graduate School of Medicine; H. Nakashima, MD, PhD; Y. Sakurai, MD, PhD, Department of Preventive Medicine and Public Health, National Defense Medical College; K. Ichida, MD, PhD, Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences; Division of Kidney and Hypertension, Department of Internal Medicine, Jikei University School of Medicine; T. Shimizu, MD, PhD, Midorigaoka Hospital; N. Shinomiya, MD, PhD, Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College.
authors.forename: Nariyoshi
authors.initials: N
authors.lastname: Shinomiya
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D062967:Cyclic GMP-Dependent Protein Kinase Type II / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006073:Gout / Q000235:genetics*; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged
chemical_list: D062967:Cyclic GMP-Dependent Protein Kinase Type II; C569729:PRKG2 protein, human
keywords: GOUTY ARTHRITIS; HYPERURICEMIA; SINGLE NUCLEOTIDE POLYMORPHISMS; URATE; URIC ACID