title: Cell banking for pharmaceutical research.
abstract: The provision of high-quality eukaryotic cells through robust cell banking processes is essential for the progression of drug discovery projects throughout the pharmaceutical research process. Numerous models exist to meet this aim, and this review describes many of the underlying principles, challenges and opportunities as well as detailing how these have been addressed within AstraZeneca. Crucial aspects discussed include cell line acquisition, cell bank generation, cryopreservation, storage, tracking and distribution. Because quality assurance underpins much of the process, quality control (QC) testing including mycoplasma screening and cell line authentication are also discussed in detail. Furthermore, because many of the underlying principles of cell banking are applicable in non-pharmaceutical settings, it is hoped that this review will prove a useful resource across the wider scientific community.
authors.affiliation: Discovery Sciences, AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK. Electronic address: jonathan.wrigley@astrazeneca.com.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Wrigley
authors.affiliation: Discovery Sciences, AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK.
authors.forename: Eileen J
authors.initials: EJ
authors.lastname: McCall
authors.affiliation: Discovery Sciences, AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK.
authors.forename: Claire L
authors.initials: CL
authors.lastname: Bannaghan
authors.affiliation: Discovery Sciences, AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK.
authors.forename: Laura
authors.initials: L
authors.lastname: Liggins
authors.affiliation: Discovery Sciences, AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK.
authors.forename: Clare
authors.initials: C
authors.lastname: Kendrick
authors.affiliation: Discovery Sciences, AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK.
authors.forename: Alison
authors.initials: A
authors.lastname: Griffen
authors.affiliation: Discovery Sciences, AstraZeneca Research & Development, Pepparedsleden 1, SE-431 83 Mölndal, Sweden.
authors.forename: Ryan
authors.initials: R
authors.lastname: Hicks
authors.affiliation: Discovery Sciences, AstraZeneca Research & Development, Pepparedsleden 1, SE-431 83 Mölndal, Sweden.
authors.forename: Linda
authors.initials: L
authors.lastname: Fröderberg-Roth
mesh_terms: D018070:Biological Specimen Banks; D035843:Biomedical Research; D002460:Cell Line; D006801:Humans; D013048:Specimen Handling