title: Novel molecular beacon probe-based real-time RT-PCR assay for diagnosis of Crimean-Congo hemorrhagic fever encountered in India.
abstract: Crimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India and requires immediate detection of infection both for preventing further transmission and for controlling the infection. The present study describes development, optimization, and evaluation of a novel molecular beacon-based real-time RT-PCR assay for rapid, sensitive, and specific diagnosis of Crimean-Congo hemorrhagic fever virus (CCHFV). The developed assay was found to be a better alternative to the reported TaqMan assay for routine diagnosis of CCHF.
authors.affiliation: High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, India.
authors.forename: Aman
authors.initials: A
authors.lastname: Kamboj
authors.affiliation: High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, India.
authors.forename: Atul Kumar
authors.initials: AK
authors.lastname: Pateriya
authors.affiliation: High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, India.
authors.forename: Anamika
authors.initials: A
authors.lastname: Mishra
authors.affiliation: High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, India.
authors.forename: Pradip
authors.initials: P
authors.lastname: Ranaware
authors.affiliation: High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, India.
authors.forename: Diwakar D
authors.initials: DD
authors.lastname: Kulkarni
authors.affiliation: High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, India.
authors.forename: Ashwin Ashok
authors.initials: AA
authors.lastname: Raut
mesh_terms: D000818:Animals; D002020:Buffaloes; D002417:Cattle; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics*; D006479:Hemorrhagic Fever, Crimean / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D007194:India; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012756:Sheep
chemical_list: D012367:RNA, Viral