title: Comparative study of placental α-microglobulin-1, insulin-like growth factor binding protein-1 and nitrazine test to diagnose premature rupture of membranes: a randomized controlled trial.
abstract: OBJECTIVE
  The aim of this study was to compare the accuracy of placental α-microglobulin-1 (PAMG-1), insulin-like growth factor binding protein-1 (IGFBP-1) and nitrazine test to diagnose premature rupture of membranes.
  
  
  METHODS
  A total of 120 pregnant women between 11 and 42 weeks with signs/symptoms of membrane rupture were eligible for our study. These women were evaluated with the PAMG-1, IGFBP-1, and nitrazine tests.
  
  
  RESULTS
  In the 120 women, the sensitivity, specificity, positive predictive value, and negative predictive value of PAMG-1, IGFBP-1 and nitrazine test were 100%, 100%, 100%, and 100%, 93.33%, 98.89%, 96.55% and 97.80%, and 93.33%, 94.44%, 84.85%, and 97.7%, respectively. In a comparison of the PAMG-1 test and the nitrazine test, positive coincidence rate was 84.85%, negative coincidence rate was 97.70%, total coincidence rate was 94.17%, and kappa value was 0.85. In a comparison of the PAMG-1 test and the IGFBP-1 test, the positive coincidence rate, negative coincidence rate and total coincidence rate were 96.55%, 97.80%, and 97.50%, and kappa value was 0.93.
  
  
  CONCLUSIONS
  PAMG-1 assay was the most accurate method to diagnose premature rupture of membranes with the highest sensitivity, specificity, positive predictive value and negative predictive value.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: De-ku
authors.initials: DK
authors.lastname: Liang
authors.forename: Hong-bo
authors.initials: HB
authors.lastname: Qi
authors.forename: Xin
authors.initials: X
authors.lastname: Luo
authors.forename: Xiao-qiu
authors.initials: XQ
authors.lastname: Xiao
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Jia
mesh_terms: D001391:Azo Compounds; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000175:diagnosis*; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000032:analysis*; D011237:Predictive Value of Tests; D011247:Pregnancy; D011446:Prospective Studies
chemical_list: D001391:Azo Compounds; C032231:IGFBP1 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1; C013617:nitrazine yellow
keywords: insulin-like growth factor binding protein-1; nitrazine test; placental α-microglobulin-1; pregnancy; premature rupture of membranes