title: Viral persistence in colorectal cancer cells infected by Newcastle disease virus.
abstract: BACKGROUND
  Newcastle disease virus (NDV), a single-stranded RNA virus of the family Paramyxoviridae, is a candidate virotherapy agent in cancer treatment. Promising responses were observed in clinical studies. Despite its high potential, the possibility of the virus to develop a persistent form of infection in cancer cells has not been investigated. Occurrence of persistent infection by NDV in cancer cells may cause the cells to be less susceptible to the virus killing. This would give rise to a population of cancer cells that remains viable and resistant to treatment.
  
  
  RESULTS
  During infection experiment in a series of colorectal cancer cell lines, we adventitiously observed a development of persistent infection by NDV in SW480 cells, but not in other cell lines tested. This cell population, designated as SW480P, showed resistancy towards NDV killing in a re-infection experiment. The SW480P cells retained NDV genome and produced virus progeny with reduced plaque forming ability.
  
  
  CONCLUSIONS
  These observations showed that NDV could develop persistent infection in cancer cells and this factor needs to be taken into consideration when using NDV in clinical settings.
authors.forename: Suet-Lin
authors.initials: SL
authors.lastname: Chia
authors.forename: Khatijah
authors.initials: K
authors.lastname: Yusoff
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia. nshafee@upm.edu.my.
authors.forename: Norazizah
authors.initials: N
authors.lastname: Shafee
mesh_terms: D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms; D006801:Humans; D009522:Newcastle disease virus / Q000254:growth & development* / Q000302:isolation & purification; D050504:Oncolytic Viruses / Q000254:growth & development* / Q000302:isolation & purification