title: TBLR1 is a novel prognostic marker and promotes epithelial-mesenchymal transition in cervical cancer.
abstract: BACKGROUND
  Invasion and metastasis remain a critical issue in cervical cancer. However, the underlying mechanism of it in cervical cancer remains unclear. The newly discovered protein, TBLR1, plays a crucial role in regulating various key cellular functions.
  
  
  METHODS
  In this study, western blot, real-time RT-PCR, immunohistochemical staining, 3D morphogenesis Matrigel culture, wound healing and Boyden chamber invasion assays, xenografted tumour model, luciferase assays, and chromatin immunoprecipitation assays were used.
  
  
  RESULTS
  The expression of TBLR1 in cervical cancer cell lines and tissues was significantly upregulated at both the RNA and protein levels compared with that in normal cervical cells. Statistical analysis suggested that TBLR1 as an independent prognostic factor was significantly correlated with the clinical stage, survival time and recurrence. Moreover, overexpression of TBLR1 in Hela and Siha cell lines promoted invasion in vitro and in vivo with the increases of the mesenchymal factors vimentin and fibronectin and decreases of the epithelial marker α-catenin. In contrast, RNAi-mediated knockdown of TBLR1 inhibited epithelial-mesenchymal transition in vitro and in vivo. Further study indicated that this might be mediated via the NF-κB and Wnt/β-Catenin signalling pathway, and involve regulation of Snail and Twist.
  
  
  CONCLUSIONS
  The TBLR1 protein may be a prognostic marker in cervical cancer and play an important role in the invasion and metastasis of human cervical cancer.
authors.affiliation: 1] Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China [2] State Key Laboratory of Oncology in Southern China, Cancer Center of Sun Yat-sen University, Guangzhou 510060, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1] Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China [2] Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Ou
authors.affiliation: 1] Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China [2] Department of Hepatic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Oncology in Southern China, Cancer Center of Sun Yat-sen University, Guangzhou 510060, People's Republic of China.
authors.forename: T
authors.initials: T
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Oncology in Southern China, Cancer Center of Sun Yat-sen University, Guangzhou 510060, People's Republic of China.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
authors.forename: M
authors.initials: M
authors.lastname: Xia
authors.affiliation: Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
authors.forename: M
authors.initials: M
authors.lastname: He
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000235:genetics; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D012189:Retrospective Studies; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D023041:Xenograft Model Antitumor Assays; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D018160:Receptors, Cytoplasmic and Nuclear; D012097:Repressor Proteins; C473691:TBL1XR1 protein, human