title: Galectin-3 contributes to luteolysis by binding to Beta 1 integrin in the bovine corpus luteum.
abstract: Luteolysis is characterized by a reduction in progesterone (P4) production and tissue degeneration in the corpus luteum (CL). One of major events during luteolysis is luteal cell death. Galectin-3, a ubiquitously expressed protein involved in many cellular processes, serves as an antiapoptotic and/or proapoptotic factor in various cell types. Although galectin-3 is detected in the bovine CL, its role remains unclear. The expression of galectin-3 in the bovine CL was higher at the regressed stage than at the other luteal stages. Galectin-3 was localized on luteal steroidogenic cells (LSCs). When cultured LSCs were exposed to prostaglandin F2alpha (PGF) for 48 h, the expression and secretion of galectin-3 increased. When the cultured LSCs were treated with galectin-3 for 24 h, cleaved caspase-3 expression was increased, and the cell viability was decreased, whereas P4 production did not change. Beta 1 integrin, a target protein of galectin-3, was expressed in bovine CL and possessed glycans, which galectin-3 binds. Furthermore, galectin-3 bound to glycans of luteal beta 1 integrin. The decreased cell viability of cultured LSCs by galectin-3 was suppressed by beta 1 integrin antibody. The overall findings suggest that the secreted galectin-3 stimulated by PGF plays a role in structural luteolysis by binding to beta 1 integrin.
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Hashiba
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sano
authors.affiliation: Laboratory of Histology and Cytology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Nio-Kobayashi
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: Takuo
authors.initials: T
authors.lastname: Hojo
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: Dariusz J
authors.initials: DJ
authors.lastname: Skarzynski
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan kokuda@cc.okayama-u.ac.jp.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okuda
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015237:Dinoprost / Q000494:pharmacology; D005260:Female; D037502:Galectin 3 / Q000378:metabolism* / Q000494:pharmacology; D019012:Integrin beta1 / Q000378:metabolism*; D008184:Luteal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003341:Luteolysis / Q000187:drug effects / Q000378:metabolism*
chemical_list: D037502:Galectin 3; D019012:Integrin beta1; D015237:Dinoprost; D053148:Caspase 3
keywords: apoptosis; corpus luteum; galectin; glycan; luteolysis