title: Inhibiting effect of Radix Hedysari Polysaccharide (HPS) on endotoxin-induced uveitis in rats.
abstract: OBJECTIVE
  The aim of this study is to investigate the anti-inflammatory effect of Radix Hedysari Polysaccharide (HPS) on clinical indicators, the expression of Toll-like receptor-4 (TLR4) and its downstream transduction molecules during endotoxin-induced uveitis in rats.
  
  
  METHODS
  EIU was induced through the intraperitoneal injection of male Wistar rats with lipopolysaccharide (LPS 200 μg). HPS (400mg/kg), DXM (1mg/kg) or an equivalent volume of normal saline was injected intraperitoneally 1h before the LPS induction. The clinical manifestation was observed and scored at 2-h intervals using a slit microscope. The degree of inflammatory reaction was determined by routine histological examinations, and the expression of TLR4 and MyD88 in the iris-ciliary body complex was detected through a double-labeled immunofluorescence study. Real-time RT-PCR was used to assess the effects of HPS on the expression of the TLR4 complex, MyD88 and NF-κB p65 mRNA. The protein expression levels of TLR4, MyD88 and NF-κB p65 were examined by western blot.
  
  
  RESULTS
  HPS treatment produced similar therapeutic results with dexamethasone by significantly reducing the clinical severity of EIU as well as fibrin exudations and inflammatory cell infiltration in the eye. Correspondingly, according to the immunofluorescence results, HPS treatment significantly suppressed the expression of TLR4 and MyD88 in the iris-ciliary body complex. HPS treatment could also remarkably reduce the mRNA and protein expression of the TLR4 complex, MyD88 and NF-κB p65.
  
  
  CONCLUSIONS
  HPS can suppress the intraocular inflammation observed in EIU by inhibiting TLR4 and its downstream signal transduction pathway.
authors.affiliation: Department of Ophthalmology, Beijing Chao Yang Hospital, Capital Medical University, No. 8 Baijiazhuang Road, Chao Yang District, Beijing 100020, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Ophthalmology, Beijing Chao Yang Hospital, Capital Medical University, No. 8 Baijiazhuang Road, Chao Yang District, Beijing 100020, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, Beijing Chao Yang Hospital, Capital Medical University, No. 8 Baijiazhuang Road, Chao Yang District, Beijing 100020, China.
authors.forename: Jinyi
authors.initials: J
authors.lastname: Yu
authors.affiliation: Lanzhou University, China.
authors.forename: Shilan
authors.initials: S
authors.lastname: Feng
authors.affiliation: Gansu Province People's Hospital, China. Electronic address: guotiankang59@126.com.
authors.forename: Tiankang
authors.initials: T
authors.lastname: Guo
authors.affiliation: Department of Ophthalmology, Beijing Chao Yang Hospital, Capital Medical University, No. 8 Baijiazhuang Road, Chao Yang District, Beijing 100020, China. Electronic address: honglu111@gmail.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004731:Endotoxins / Q000009:adverse effects*; D005123:Eye / Q000187:drug effects / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism; D014605:Uveitis / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D004731:Endotoxins; C507781:Myd88 protein, rat; D053594:Myeloid Differentiation Factor 88; D011134:Polysaccharides; D012333:RNA, Messenger; C498145:Rela protein, rat; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D051996:Transcription Factor RelA
keywords: Anti-inflammatory; Endotoxin-induced uveitis; Radix Hedysari Polysaccharide; TLR-4