title: New applications for phage integrases.
abstract: Within the last 25 years, bacteriophage integrases have rapidly risen to prominence as genetic tools for a wide range of applications from basic cloning to genome engineering. Serine integrases such as that from ϕC31 and its relatives have found an especially wide range of applications within diverse micro-organisms right through to multi-cellular eukaryotes. Here, we review the mechanisms of the two major families of integrases, the tyrosine and serine integrases, and the advantages and disadvantages of each type as they are applied in genome engineering and synthetic biology. In particular, we focus on the new areas of metabolic pathway construction and optimization, biocomputing, heterologous expression and multiplexed assembly techniques. Integrases are versatile and efficient tools that can be used in conjunction with the various extant molecular biology tools to streamline the synthetic biology production line.
authors.affiliation: Department of Biology, University of York, Wentworth Way, York YO10 5DD, UK.
authors.forename: Paul C M
authors.initials: PC
authors.lastname: Fogg
authors.affiliation: Institute of Molecular Cell and Systems Biology, University of Glasgow, Bower Building, Glasgow G12 8QQ, UK.
authors.forename: Sean
authors.initials: S
authors.lastname: Colloms
authors.affiliation: School of Biological Sciences, University of Edinburgh, King's Building, Edinburgh EH9 3JR, UK.
authors.forename: Susan
authors.initials: S
authors.lastname: Rosser
authors.affiliation: Institute of Molecular Cell and Systems Biology, University of Glasgow, Bower Building, Glasgow G12 8QQ, UK.
authors.forename: Marshall
authors.initials: M
authors.lastname: Stark
authors.affiliation: Department of Biology, University of York, Wentworth Way, York YO10 5DD, UK. Electronic address: maggie.smith@york.ac.uk.
authors.forename: Margaret C M
authors.initials: MC
authors.lastname: Smith
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000201:enzymology*; D005818:Genetic Engineering; D006801:Humans; D019426:Integrases / Q000235:genetics*
chemical_list: D019426:Integrases
keywords: bacteriophages; genome engineering; integrases; integrating vectors; synthetic biology