title: Comparative studies on the heterogeneity of plasma-derived and recombinant human albumins in laboratory use.
abstract: We investigated the thiol-redox state, and the relationship between structural characteristics, such as thermal stability, and functional properties, such as cell growth activity, of commercial plasma-derived (pHSA) and recombinant human serum albumin (rHSA) products. In this study, 5 pHSA products (A1653, A9511, A1887, A8763, and A3782) and 2 rHSA products (A9731 and A9986) were obtained from Sigma-Aldrich. Among them, three kinds of HSA products [A1653 (an initial fractionation product), A3782 (a final purified product), and A9731 (recombinant albumin expressed in rice)] were selected for experiments on the thermal stabilities, analyzed by thermal denaturation curves, and cell growth activities of U937 and THP-1 cell lines using the WST-1 reagent. The secondary and tertiary structures of HSA products were similar, whereas a marked difference was observed in their thermal stabilities. The degree of thermal stability of the three representative products was in the order of A9731 (rHSA)>A1653 (pHSA)>A3782 (pHSA), as was the degree of cell growth activity of these products. One possible explanation for the present results is that albumin-bound fatty acids may have influenced the thermal stabilities and cell growth activities of U937 and THP-1 cells.
authors.affiliation: Department of Physiology and Biophysics, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Minami
authors.affiliation: Department of Physiology and Biophysics, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Terada
authors.affiliation: Department of Physiology and Biophysics, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Department of Physiology and Biophysics, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Arikawa
authors.affiliation: Department of Physiology and Biophysics, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Matsuyama
authors.affiliation: Department of Physiology and Biophysics, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
authors.forename: Kazuha
authors.initials: K
authors.lastname: Kizaki
authors.affiliation: Department of Physiology and Biophysics, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan. Electronic address: era@gifu-u.ac.jp.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Era
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007753:Laboratories; D010084:Oxidation-Reduction; D011487:Protein Conformation; D055503:Protein Multimerization; D055550:Protein Stability; D011994:Recombinant Proteins / Q000737:chemistry* / Q000494:pharmacology*; D012709:Serum Albumin / Q000737:chemistry* / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013696:Temperature
chemical_list: D011994:Recombinant Proteins; D012709:Serum Albumin; D013438:Sulfhydryl Compounds
keywords: Cell culture; Commercial product; Heterogeneity; Redox state; Serum albumin; Thermal stability