title: HIP-55 negatively regulates myocardial contractility at the single-cell level.
abstract: Myocardial contractility is crucial for cardiac output and heart function. But the detailed mechanisms of regulation remain unclear. In the present study, we found that HIP-55, an actin binding protein, negatively regulates myocardial contractility at the single-cell level. HIP-55 was overexpressed and knocked down in cardiomyocytes with an adenovirus infection. The traction forces exerted by single cardiomyocyte were measured using cell traction force microscopy. The results showed that HIP-55 knockdown significantly increased the contractility of the cardiomyocytes and HIP-55 overexpression could markedly reverse this process. Furthermore, HIP-55 was obviously co-localized with F-actin in cardiomyocytes, suggesting that HIP-55 regulated cardiac contractile function through the interaction between HIP-55 and F-actin. This study reveals the regulatory mechanisms of myocardial contractility and provides a new target for preventing and treating cardiovascular disease.
authors.affiliation: Institute of Vascular Medicine, Peking University Third Hospital, Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education and Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.affiliation: Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.affiliation: Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Engineering, Peking University, Beijing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: College of Engineering, Peking University, Beijing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Engineering, Peking University, Beijing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Deng
authors.affiliation: Institute of Vascular Medicine, Peking University Third Hospital, Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education and Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Yang
authors.affiliation: College of Engineering, Peking University, Beijing, China.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Huang
authors.affiliation: Institute of Vascular Medicine, Peking University Third Hospital, Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education and Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
authors.forename: Youyi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China; College of Engineering, Peking University, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.affiliation: Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China; College of Engineering, Peking University, Beijing, China. Electronic address: cyxiong@pku.edu.cn.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Institute of Vascular Medicine, Peking University Third Hospital, Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education and Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, China. Electronic address: lizijian@bjmu.edu.cn.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Li
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology; D000199:Actins / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D008840:Microfilament Proteins / Q000378:metabolism / Q000502:physiology*; D018613:Microscopy, Confocal; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018909:src Homology Domains / Q000502:physiology*
chemical_list: D000199:Actins; C485513:Dbnl protein, rat; D008840:Microfilament Proteins
keywords: HIP-55; Myocardial contractility; Traction force microscopy