title: Multiplex hydrolysis probe real-time PCR for simultaneous detection of hepatitis A virus and hepatitis E virus.
abstract: Detection of hepatitis viral infections has traditionally relied on the circulating antibody test using the enzyme-linked immunosorbent assay. However, multiplex real-time PCR has been increasingly used for a variety of viral nucleic acid detections and has proven to be superior to traditional methods. Hepatitis A virus (HAV) and hepatitis E virus (HEV) are the major causes of acute hepatitis worldwide; both HAV and HEV infection are a main public health problem. In the present study, a one-step multiplex reverse transcriptase quantitative polymerase chain reaction assay using hydrolysis probes was developed for simultaneously detecting HAV and HEV. This novel detection system proved specific to the target viruses, to be highly sensitive and to be applicable to clinical sera samples, making it useful for rapid, accurate and feasible identification of HAV and HEV.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China. qiufeng615@sina.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China. caojy@126.com.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Cao
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China. 13426329820@163.com.
authors.forename: Qiudong
authors.initials: Q
authors.lastname: Su
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China. yyao112@163.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China. shengli_bi@163.com.
authors.forename: Shengli
authors.initials: S
authors.lastname: Bi
mesh_terms: D006506:Hepatitis A / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D030041:Hepatitis A virus / Q000235:genetics / Q000302:isolation & purification*; D016751:Hepatitis E / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D016752:Hepatitis E virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D006868:Hydrolysis; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral