title: Angle-resolved light scattering of individual rod-shaped bacteria based on Fourier transform light scattering.
abstract: Two-dimensional angle-resolved light scattering maps of individual rod-shaped bacteria are measured at the single-cell level. Using quantitative phase imaging and Fourier transform light scattering techniques, the light scattering patterns of individual bacteria in four rod-shaped species (Bacillus subtilis, Lactobacillus casei, Synechococcus elongatus, and Escherichia coli) are measured with unprecedented sensitivity in a broad angular range from -70° to 70°. The measured light scattering patterns are analyzed along the two principal axes of rod-shaped bacteria in order to systematically investigate the species-specific characteristics of anisotropic light scattering. In addition, the cellular dry mass of individual bacteria is calculated and used to demonstrate that the cell-to-cell variations in light scattering within bacterial species is related to the cellular dry mass and growth.
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: YoungJu
authors.initials: Y
authors.lastname: Jo
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: JaeHwang
authors.initials: J
authors.lastname: Jung
authors.affiliation: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Jee Woong
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Materials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Della
authors.initials: D
authors.lastname: Shin
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: HyunJoo
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Materials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Ki Tae
authors.initials: KT
authors.lastname: Nam
authors.affiliation: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Ji-Ho
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: YongKeun
authors.initials: Y
authors.lastname: Park
mesh_terms: D016880:Anisotropy; D001412:Bacillus subtilis / Q000737:chemistry* / Q000145:classification; D004926:Escherichia coli / Q000737:chemistry* / Q000145:classification; D007780:Lacticaseibacillus casei / Q000737:chemistry* / Q000145:classification; D008027:Light; D059010:Single-Cell Analysis; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared; D046940:Synechococcus / Q000737:chemistry* / Q000145:classification