title: Copy number variation of the beta defensin gene cluster on chromosome 8p influences the bacterial microbiota within the nasopharynx of otitis-prone children.
abstract: As there is increasing evidence that aberrant defensin expression is related to susceptibility for infectious disease and inflammatory disorders, we sought to determine if copy number of the beta-defensin gene cluster located on chromosome 8p23.1 (DEFB107, 106, 105, 104, 103, DEFB4 and SPAG11), that shows copy number variation as a block, was associated with susceptibility to otitis media (OM). The gene DEFB103 within this complex encodes human beta defensin-3 (hBD-3), an antimicrobial peptide (AP) expressed by epithelial cells that line the mammalian airway, important for defense of mucosal surfaces and previously shown to have bactericidal activity in vitro against multiple human pathogens, including the three that predominate in OM. To this end, we conducted a retrospective case-control study of 113 OM prone children and 267 controls aged five to sixty months. We identified the copy number of the above defined beta-defensin gene cluster (DEFB-CN) in each study subject by paralogue ratio assays. The mean DEFB-CN was indistinguishable between subjects classified as OM prone based on a recent history of multiple episodes of OM and control subjects who had no history of OM (4.4 ± 0.96 versus 4.4 ± 1.08, respectively: Odds Ratio [OR]: 1.16 (95% CI: 0.61, 2.20). Despite a lack of direct association, we observed a statistically significant correlation between DEFB-CN and nasopharyngeal bacterial colonization patterns. Collectively, our findings suggested that susceptibility to OM might be mediated by genetic variation among individuals, wherein a DEFB-CN less than 4 exerts a marked influence on the microbiota of the nasopharynx, specifically with regard to colonization by the three predominant bacterial pathogens of OM.
authors.affiliation: Center for Microbial Pathogenesis, Department of Pediatrics, College of Medicine, The Research Institute at Nationwide Children's Hospital, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Jones
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Anchasa
authors.initials: A
authors.lastname: Kananurak
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Charles L
authors.initials: CL
authors.lastname: Bevins
authors.affiliation: Department of Genetics, University of Leicester, Leicester, United Kingdom.
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Hollox
authors.affiliation: Center for Microbial Pathogenesis, Department of Pediatrics, College of Medicine, The Research Institute at Nationwide Children's Hospital, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Lauren O
authors.initials: LO
authors.lastname: Bakaletz
mesh_terms: D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D016022:Case-Control Studies; D002675:Child, Preschool; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D018628:Gene Dosage; D006801:Humans; D007223:Infant; D064307:Microbiota; D005810:Multigene Family; D009305:Nasopharynx / Q000382:microbiology*; D010033:Otitis Media / Q000235:genetics* / Q000382:microbiology; D012189:Retrospective Studies; D012307:Risk Factors; D023083:beta-Defensins / Q000235:genetics*
chemical_list: D023083:beta-Defensins