title: Effects of erythromycin on γ-glutamyl cysteine synthetase and interleukin-1β in hyperoxia-exposed lung tissue of premature newborn rats.
abstract: OBJECTIVE
  To explore the effect of erythromycin on hyperoxia-induced lung injury.
  
  
  METHODS
  One-day-old preterm offspring Sprague-Dawley (SD) rats were randomly divided into four groups: group 1, air + sodium chloride; group 2, air + erythromycin;group 3, hyperoxia + sodium chloride; and group 4, hyperoxia + erythromycin. At one, seven, and 14 days of exposure, glutathione (GSH) and interleukin-1 beta (IL-1 beta) were detected by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA), and bicinchoninic acid (BCA) was used to detect GSH protein. γ-glutamine-cysteine synthetase (γ-GCS) mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Compared with group 1, expressions of GSH and γ-GCS mRNA in group 3 were significantly increased at one and seven days of exposure (p < 0.05), but expression of γ-GCS mRNA was significantly reduced at 14 days; expression of IL-1 beta in group 3 was significantly increased at seven days of exposure (p < 0.05), and was significantly reduced at 14 days. Compared with group 3, expressions of GSH and γ-GCS mRNA in group 4 were significantly increased at one, seven, and 14 days of exposure (p < 0.05), but expressions of GSH showed a downward trend at 14 days; expression of IL-1 beta in group 4 was significantly reduced at one and seven days of exposure (p < 0.05).
  
  
  CONCLUSIONS
  Changes in oxidant-mediated IL-1 beta and GSH are involved in the development of hyperoxia-induced lung injury. Erythromycin may up-regulate the activity of γ-GCS, increasing the expression of GSH, inhibiting the levels of oxidant-mediated IL-1 beta and alleviating hyperoxia-induced lung injury via an antioxidant effect.
authors.affiliation: Department of Neonatology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China. Electronic address: caicheng2004@163.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cai
authors.affiliation: Department of Neonatology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Qiu
authors.affiliation: Department of Neonatology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Gong
authors.affiliation: Department of Neonatology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Yihuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Chinese Minority Ethnic Groups' Traditional Medicine Research Center, Central University for Nationalities, Beijing, China.
authors.forename: Huanhu
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004917:Erythromycin / Q000494:pharmacology*; D005260:Female; D005721:Glutamate-Cysteine Ligase / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D018496:Hyperoxia / Q000378:metabolism*; D053583:Interleukin-1beta / Q000187:drug effects* / Q000378:metabolism; D008168:Lung / Q000187:drug effects*; D055370:Lung Injury / Q000378:metabolism; D008297:Male; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D053583:Interleukin-1beta; D011500:Protein Synthesis Inhibitors; D004917:Erythromycin; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione; D010100:Oxygen
keywords: Eritromicina; Erythromycin; Glutathione; Glutationa; Hiperóxia; Hyperoxia; Interleucina-1 beta; Interleukin-1-beta; Lesão pulmonar; Lung injury