title: Effects of repeated administration of methylphenidate on reproductive parameters in male rats.
abstract: Methylphenidate (MPH) is a psychostimulant drug which acts by blocking the dopamine and norepinephrine transporters and is the main drug used to treat attention deficit hyperactivity disorder in children and adolescents. During puberty, changes in neurotransmitter systems (including dopaminergic system) are engaged on the release of gonadal hormones and the development of cephalic structures responsible for reproductive function. This study investigated the effects of repeated treatment with methylphenidate during development on reproductive parameters of adult male rats. Wistar rats received MPH 2.5 mg/kg, MPH 5.0 mg/kg, or distilled water (gavage) from postnatal day (PND) 21 to PND 60. At PND 100, an increase in percentage of abnormal tail morphology sperm in MPH 2.5 and increase in testicular interstitial tissue volume in MPH groups as well as in the number of type A spermatogonia in MPH 5.0 group were observed. This study demonstrated that repeated administration of methylphenidate during periods corresponding childhood to early adulthood interfered on testicular function in rats at adult life.
authors.affiliation: Department of Physiological Sciences, State University of Londrina, 86051-980 Londrina, Paraná, Brazil.
authors.forename: Bruno Garcia
authors.initials: BG
authors.lastname: Montagnini
authors.affiliation: Department of Physiological Sciences, State University of Londrina, 86051-980 Londrina, Paraná, Brazil.
authors.forename: Luiza Sienna
authors.initials: LS
authors.lastname: Silva
authors.affiliation: Department of Physiological Sciences, State University of Londrina, 86051-980 Londrina, Paraná, Brazil.
authors.forename: Alice Hartmann
authors.initials: AH
authors.lastname: dos Santos
authors.affiliation: Department of Morphology, Estomatology and Physiology, University of São Paulo, 14040-900 Ribeirão Preto, São Paulo, Brazil.
authors.forename: Janete Aparecida
authors.initials: JA
authors.lastname: Anselmo-Franci
authors.affiliation: Department of Biology, State University of Londrina, 86051-980 Londrina, Paraná, Brazil.
authors.forename: Glaura Scantamburlo Alves
authors.initials: GS
authors.lastname: Fernandes
authors.affiliation: Department of Biology, State University of Londrina, 86051-980 Londrina, Paraná, Brazil.
authors.forename: Suzana de Fátima Paccola
authors.initials: Sde F
authors.lastname: Mesquita
authors.affiliation: Department of Physiological Sciences, State University of Londrina, 86051-980 Londrina, Paraná, Brazil. Electronic address: dcgerardin@uel.br.
authors.forename: Daniela Cristina Ceccatto
authors.initials: DC
authors.lastname: Gerardin
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects; D000697:Central Nervous System Stimulants / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004822:Epididymis / Q000187:drug effects; D005260:Female; D005837:Genitalia, Male / Q000187:drug effects*; D008297:Male; D008774:Methylphenidate / Q000008:administration & dosage*; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects*; D046468:Sexual Development / Q000187:drug effects*; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013737:Testis / Q000187:drug effects; D013739:Testosterone / Q000097:blood
chemical_list: D000697:Central Nervous System Stimulants; D008774:Methylphenidate; D013739:Testosterone
keywords: Attention-deficit/hyperactivity disorder; Development; Methylphenidate; Sperm morphology; Spermatogenesis; Testicle