title: δ-Aminolevulinate dehydratase activity in lung cancer patients and its relationship with oxidative stress.
abstract: This study investigated the δ-aminolevulinate dehydratase (δ-ALA-D) activity in whole blood as well as the parameters of oxidative stress, such as reactive species (RS) levels in serum, thiobarbituric acid reactive substances (TBARS) levels, the superoxide dismutase (SOD) and catalase (CAT) activities, as well as total thiols (T-SH) and non-protein thiols (NPSH) levels in platelets. Moreover, the content of vitamin C and E in plasma and serum, respectively, in lung cancer patients was also investigated. We collected blood samples from patients (n=28) previously treated for lung cancer with chemotherapy. Patients were classified as stage IIIb and IV according to the Union for International Cancer Control (UICC). Results showed a decrease of 37% in δ-ALA-D activity in patients with lung cancer when compared to the control group. RS and TBARS levels were 8% and 99% higher in the patient group, respectively. The activity of SOD and CAT as well as the vitamin C content were 41%, 35% and 127% lower in patients when compared with controls, respectively. However, T-SH and vitamin E levels were 27% and 44% higher in lung cancer patients, respectively. Results show that the overproduction of reactive species in patients with lung cancer may be interfering with the activity of δ-ALA-D. Likewise, the decrease in the activity of this enzyme may be contributing for the oxidative stress.
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil. Electronic address: dz_daniela@yahoo.com.br.
authors.forename: Daniela
authors.initials: D
authors.lastname: Zanini
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Luana Paula
authors.initials: LP
authors.lastname: Pelinson
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Luciane
authors.initials: L
authors.lastname: Belmonte Pereira
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Curry Martins
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Jucimara
authors.initials: J
authors.lastname: Baldissareli
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Pires Amaral
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Félix Alexandre
authors.initials: FA
authors.lastname: Antunes Soares
authors.affiliation: Setor de Hematologia/Oncologia, Hospital Universitário de Santa Maria, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Luiz Gustavo
authors.initials: LG
authors.lastname: Brenner Reetz
authors.affiliation: Setor de Hematologia/Oncologia, Hospital Universitário de Santa Maria, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Maria do Carmo
authors.initials: Mdo C
authors.lastname: Araújo
authors.affiliation: Setor de Hematologia/Oncologia, Hospital Universitário de Santa Maria, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Juarez
authors.initials: J
authors.lastname: Chiesa
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Bitencourt Rosa Leal
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil. Electronic address: mariachitolina@gmail.com.
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D000368:Aged; D001205:Ascorbic Acid / Q000097:blood; D001792:Blood Platelets / Q000201:enzymology; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D002945:Cisplatin / Q000494:pharmacology / Q000627:therapeutic use; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000097:blood; D000093542:Gemcitabine
chemical_list: D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D003841:Deoxycytidine; D014810:Vitamin E; D002374:Catalase; D013482:Superoxide Dismutase; D000623:Porphobilinogen Synthase; D001205:Ascorbic Acid; D002945:Cisplatin; D000093542:Gemcitabine
keywords: Antioxidants; Lung cancer; Oxidative stress; δ-Aminolevulinate dehydratase