title: Preparation of mesoporous silica thin films by photocalcination method and their adsorption abilities for various proteins.
abstract: Mesoporous silica (MPS) thin film biosensor platforms were established. MPS thin films were prepared from tetraethoxysilane (TEOS) via using sol-gel and spin-coating methods using a poly-(ethylene oxide)-block-poly-(propylene oxide)-block-poly-(ethylene oxide) triblock polymer, such as P123 ((EO)20(PO)70(EO)20) or F127 ((EO)106(PO)70(EO)106), as the structure-directing agent. The MPS thin film prepared using P123 as the mesoporous template and treated via vacuum ultraviolet (VUV) irradiation to remove the triblock copolymer had a more uniform pore array than that of the corresponding film prepared via thermal treatment. Protein adsorption and enzyme-linked immunosorbent assay (ELISA) on the synthesized MPS thin films were also investigated. VUV-irradiated MPS thin films adsorbed a smaller quantity of protein A than the thermally treated films; however, the human immunoglobulin G (IgG) binding efficiency was higher on the former. In addition, protein A-IgG specific binding on MPS thin films was achieved without using a blocking reagent; i.e., nonspecific adsorption was inhibited by the uniform pore arrays of the films. Furthermore, VUV-irradiated MPS thin films exhibited high sensitivity for ELISA testing, and cytochrome c adsorbed on the MPS thin films exhibited high catalytic activity and recyclability. These results suggest that MPS thin films are attractive platforms for the development of novel biosensors.
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), 2266-98 Anagahora, Shimoshidami, Moriyama-ku, Nagoya 463-8560, Japan. Electronic address: katsuya-kato@aist.go.jp.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Kato
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), 2266-98 Anagahora, Shimoshidami, Moriyama-ku, Nagoya 463-8560, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Nakamura
authors.affiliation: Department of Chemistry for Materials, Graduate School of Engineering, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8570, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamauchi
authors.affiliation: Department of Chemistry for Materials, Graduate School of Engineering, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8570, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Nakanishi
authors.affiliation: Department of Chemistry for Materials, Graduate School of Engineering, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8570, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tomita
mesh_terms: D000327:Adsorption; D055162:Biocatalysis; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005782:Gels / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry; D016062:Porosity; D011409:Propylene Glycols / Q000138:chemical synthesis / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D005782:Gels; D055767:Immobilized Proteins; D007074:Immunoglobulin G; C116176:PEO-PPO-PEO; D011409:Propylene Glycols; D012821:Silanes; D013205:Staphylococcal Protein A; D011092:Polyethylene Glycols; C040733:tetraethoxysilane; D012822:Silicon Dioxide; D045304:Cytochromes c
keywords: Antibody; ELISA; Mesoporous silica; Protein A; Thin film