title: Quantitative expression of serum biomarkers involved in angiogenesis and inflammation, in patients with glioblastoma multiforme: correlations with clinical data.
abstract: Glioblastoma multiforme (GBM) represents a very aggressive brain tumor. Angiogenesis is the formation of a network of new blood vessels, from preexisting ones. It plays an important role in the formation of the tumor, as it supplies it with oxygen and nutrients. Angiogenesis and inflammation play essential roles in glioblastoma development. These processes are regulated by the balance of a few molecules, acting as pro- or antiangiogenic and pro- or anti-inflammatory factors. The purpose of our study was to evaluate the expression of 7 markers involved in angiogenesis and inflammation pathways in patients with glioblastoma. VEGF, PDGF-bb, IGF-1, TGF-β, TNF-α, IL-6 and IL-8 levels were measured using the ELISA method, in the preoperative sera of 14 patients with histopathologically confirmed glioblastoma multiforme and 32 healthy patients. Serum levels of PDGF-bb, IGF-1 and IL-8 were significantly higher in patients with GBM, compared to the control group (p-value < 0.01). A statistically significant correlation has been found between IGF-1 and IL-6 levels (rho= -0.53, p-value < 0.05) and also between TNF-α and IL-6 levels (rho=0.60, p-value < 0.05). Statistically significant associations have been found between the presence of low levels of IL-8 and the development of coagulation necrosis (p-value < 0.05), high levels of VEGF and development of ischemic necrosis (p-value < 0.01) and high levels of IL-8 and the development of endothelial hyperplasia (p-value < 0.05). We have observed no statistically significant associations between the serum levels of the markers and the survival rates.
authors.affiliation: Department of Dermatology, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Dermatology, University of Freiburg, Freiburg, Germany.
authors.forename: Roxana
authors.initials: R
authors.lastname: Chiorean
authors.affiliation: Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", Cluj-Napoca, Romania Research Center for Functional Genomics, Biomedicine and Translational Medicine, "IuliuHatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Immunology, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Ioana
authors.initials: I
authors.lastname: Berindan-Neagoe
authors.affiliation: Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", Cluj-Napoca, Romania Research Center for Functional Genomics, Biomedicine and Translational Medicine, "IuliuHatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Braicu
authors.affiliation: Department of Neurosurgery, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca,Romania.
authors.forename: Ioan Stefan
authors.initials: IS
authors.lastname: Florian
authors.affiliation: Department of Medical Informatics and Biostatistics, "Iuliu Hatieganu" University of Medicine andPharmacy, Cluj-Napoca, Romania.
authors.forename: Daniel
authors.initials: D
authors.lastname: Leucuta
authors.affiliation: Department of Pathology, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca,Romania.
authors.forename: Doinita
authors.initials: D
authors.lastname: Crisan
authors.affiliation: Department of Radiotherapy, The Oncology Institute "Prof. Dr. Ion Chiricuta", Cluj-Napoca,Romania Department of Oncology, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca,Romania.
authors.forename: Valentin
authors.initials: V
authors.lastname: Cernea
mesh_terms: D000077214:Becaplermin; D015415:Biomarkers / Q000097:blood*; D001932:Brain Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D016022:Case-Control Studies; D018572:Disease-Free Survival; D005909:Glioblastoma / Q000097:blood / Q000378:metabolism* / Q000401:mortality; D006801:Humans; D007249:Inflammation / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D018919:Neovascularization, Physiologic / Q000502:physiology*; D011237:Predictive Value of Tests; D020574:Proto-Oncogene Proteins c-sis / Q000097:blood; D016212:Transforming Growth Factor beta / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: D015415:Biomarkers; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D020574:Proto-Oncogene Proteins c-sis; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D000077214:Becaplermin; D007334:Insulin-Like Growth Factor I
keywords: ELISA; Glioblastoma multiforme; angiogenesis; histopathology; inflammation; survival rates