title: Electrochemical methods for detection of post-translational modifications of proteins.
abstract: Post-translational modifications of proteins play a key role in the regulation of various cellular processes. The analysis and identification of post-translational modifications are probably the most versatile and difficult, but also most frequently studied area of interest in proteomics research. This review focuses on the electroactivity of amino acids as a tool for analysis of post-translational modifications of proteins. The most attention is paid to the electrochemical detection of phosphorylation/dephosphorylation and glycosylation of proteins, to the best-studied and functionally-significant modifications, and, also, to the electrochemical analysis of activity of enzymes responsible for carrying out phosphorylation/dephosphorylation of proteins. Recent advances in electrochemistry with special references to proteomics are outlined and innovative technologies for protein detection are highlighted.
authors.affiliation: Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya St. 10/8, Moscow 119121, Russia. Electronic address: viktoria.shumyantseva@ibmc.msk.ru.
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Shumyantseva
authors.affiliation: Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya St. 10/8, Moscow 119121, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Suprun
authors.affiliation: Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya St. 10/8, Moscow 119121, Russia.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Bulko
authors.affiliation: Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya St. 10/8, Moscow 119121, Russia.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D011506:Proteins
keywords: Biosensors; Electrochemistry; Glycosylation; Phosphorylation; Post-translational modifications