title: Plasma proteomic analysis of patients infected with H1N1 influenza virus.
abstract: This study profiled the plasma proteins of patients infected by the 2011 H1N1 influenza virus. Differential protein expression was identified in plasma obtained from noninfected control subjects (n = 15) and H1N1-infected subjects (n = 15). Plasma proteins were separated by a 2DE large gel system and identified by nano-ultra performance LC-MS. Western blot assays were performed to validate proteins. Eight plasma proteins were upregulated and six proteins were downregulated among 3316 plasma proteins in the H1N1-infected group as compared with the control group. Of 14 up- and downregulated proteins, nine plasma proteins were validated by Western blot analysis. Putative protein FAM 157A, leucine-rich alpha 2 glycoprotein, serum amyloid A protein, and dual oxidase 1 showed significant differential expression. The identified plasma proteins could be potential candidates for biomarkers of H1N1 influenza viral infection. Further studies are needed to develop these proteins as diagnostic biomarkers.
authors.affiliation: Graduate School of Medicine, Korea University, Seoul, Republic of Korea.
authors.forename: Seonyoung
authors.initials: S
authors.lastname: Choi
authors.forename: Ji-youn
authors.initials: JY
authors.lastname: Lim
authors.forename: Yoonjin
authors.initials: Y
authors.lastname: Kim
authors.forename: Moon Jung
authors.initials: MJ
authors.lastname: Song
authors.forename: Woon-Won
authors.initials: WW
authors.lastname: Jung
authors.forename: Jung Bok
authors.initials: JB
authors.lastname: Seo
authors.forename: Jacob
authors.initials: J
authors.lastname: Lee
authors.forename: Donggeun
authors.initials: D
authors.lastname: Sul
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis* / Q000378:metabolism; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D054884:Host-Pathogen Interactions; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000502:physiology*; D007251:Influenza, Human / Q000097:blood* / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D040901:Proteomics
chemical_list: D001798:Blood Proteins
keywords: Biomarkers; Biomedicine; H1N1; Influenza virus; Plasma proteins