title: Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction.
abstract: This paper reports on the modification of magnetic beads with oligonucleotide capture probes with a specially designed pendant toehold (overhang) aimed specifically to capture double-stranded PCR products. After capture, the PCR products were selectively released from the magnetic beads by means of a toehold-mediated strand displacement reaction using short artificial oligonucleotide triggers and analysed using capillary electrophoresis. The approach was successfully shown on two genes widely used in human DNA genotyping, namely human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO) and amelogenin.
authors.affiliation: Flinders Centre for Nanoscale Science and Technology, Flinders University, GPO Box 2100, Adelaide, SA, 5001 Australia. dmitriy.khodakov@flinders.edu.au amanda.ellis@fliders.edu.au.
authors.forename: Dmitriy A
authors.initials: DA
authors.lastname: Khodakov
authors.forename: Anastasia S
authors.initials: AS
authors.lastname: Khodakova
authors.forename: Adrian
authors.initials: A
authors.lastname: Linacre
authors.forename: Amanda V
authors.initials: AV
authors.lastname: Ellis
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics; D019075:Electrophoresis, Capillary; D016236:Genes, fms; D060005:Genotyping Techniques; D006801:Humans; D060328:Magnetic Phenomena; D059346:Magnets / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics*
chemical_list: C000715470:MAS1 protein, human; D015345:Oligonucleotide Probes; D000090063:Proto-Oncogene Mas; D004247:DNA; D016186:Receptor, Macrophage Colony-Stimulating Factor