title: Functional magnetic resonance microscopy at single-cell resolution in Aplysia californica.
abstract: In this work, we show the feasibility of performing functional MRI studies with single-cell resolution. At ultrahigh magnetic field, manganese-enhanced magnetic resonance microscopy allows the identification of most motor neurons in the buccal network of Aplysia at low, nontoxic Mn(2+) concentrations. We establish that Mn(2+) accumulates intracellularly on injection into the living Aplysia and that its concentration increases when the animals are presented with a sensory stimulus. We also show that we can distinguish between neuronal activities elicited by different types of stimuli. This method opens up a new avenue into probing the functional organization and plasticity of neuronal networks involved in goal-directed behaviors with single-cell resolution.
authors.affiliation: NeuroSpin, Commissariat à l'Energie Atomique et aux Energies Alternatives, 91191 Gif-sur-Yvette, France; and.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Radecki
authors.affiliation: Université de Bordeaux andCentre National de la Recherche Scientifique, Institut de Neurosciences Cognitives et Intégratives d'Aquitaine, Unité Mixte de Recherche 5287, F-33000 Bordeaux, France.
authors.forename: Romuald
authors.initials: R
authors.lastname: Nargeot
authors.affiliation: NeuroSpin, Commissariat à l'Energie Atomique et aux Energies Alternatives, 91191 Gif-sur-Yvette, France; and.
authors.forename: Ileana Ozana
authors.initials: IO
authors.lastname: Jelescu
authors.affiliation: NeuroSpin, Commissariat à l'Energie Atomique et aux Energies Alternatives, 91191 Gif-sur-Yvette, France; and.
authors.forename: Denis
authors.initials: D
authors.lastname: Le Bihan
authors.affiliation: NeuroSpin, Commissariat à l'Energie Atomique et aux Energies Alternatives, 91191 Gif-sur-Yvette, France; and luisa.ciobanu@cea.fr.
authors.forename: Luisa
authors.initials: L
authors.lastname: Ciobanu
mesh_terms: D000818:Animals; D001048:Aplysia / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D001070:Appetitive Behavior / Q000502:physiology; D005240:Feasibility Studies; D005247:Feeding Behavior / Q000502:physiology; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008345:Manganese / Q000378:metabolism / Q000493:pharmacokinetics; D009046:Motor Neurons / Q000378:metabolism / Q000502:physiology*; D015203:Reproducibility of Results; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D008345:Manganese
keywords: manganese-enhanced MRI; neuroimaging