title: Environmental determinants of the spatial distribution of Trichinella britovi and Trichinella spiralis in Hungary.
abstract: Trichinella spiralis and Trichinella britovi are the two most common species of the genus Trichinella persisting in the European wildlife. To investigate the spatial distribution of these Trichinella spp. and the factors influencing their circulation in Hungary, 3304 red foxes (Vulpes vulpes) and 0.29 million wild boars (Sus scrofa) were tested for Trichinella sp. infection in Hungary from 2006 to 2013. Trichinella spp. larvae from 68 (2.06%) foxes and 44 (0.015%) wild boars were identified by a multiplex PCR as T. britovi or T. spiralis. The locality of origin of foxes and wild boars were recorded in a geographic information system database. There was no correlation between environmental parameters in the home range of foxes and wild boars and the T. spiralis larval counts, but there was a positive correlation between the boundary zone of Hungary and T. spiralis infection (P<0.0001; odds ratio: 24.1). These results indicate that the distribution of T. spiralis in the Hungarian wildlife is determined by the transborder transmission of the parasite from the surrounding endemic countries. Multiple regression analysis was performed with environmental parameter values and T. britovi larval counts. Based on the statistical analysis, non-agricultural areas (forests, scrubs, herbaceous vegetation and pastures) and the mean annual temperature (P<0.0001; odds ratios: 9.53 and 0.61) were the major determinants of the spatial distribution of T. britovi in Hungary. The positive relationship with non-agricultural areas can be explained by the generalist feeding behaviour including scavenging of foxes in these areas. The negative relationship with the mean annual temperature can be attributed to the slower decomposition of wildlife carcasses favouring a longer survival of T. britovi larvae in the host carrion and to the increase of scavenging of foxes.
authors.affiliation: Laboratory of Parasitology, Fish and Bee Diseases, Veterinary Diagnostic Directorate, National Food Chain Safety Office, Tábornok utca 2, H-1143 Budapest, Hungary.
authors.forename: Z
authors.initials: Z
authors.lastname: Tolnai
authors.affiliation: Laboratory of Parasitology, Fish and Bee Diseases, Veterinary Diagnostic Directorate, National Food Chain Safety Office, Tábornok utca 2, H-1143 Budapest, Hungary.
authors.forename: Z
authors.initials: Z
authors.lastname: Széll
authors.affiliation: Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanitá, viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Marucci
authors.affiliation: Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanitá, viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Pozio
authors.affiliation: Laboratory of Parasitology, Fish and Bee Diseases, Veterinary Diagnostic Directorate, National Food Chain Safety Office, Tábornok utca 2, H-1143 Budapest, Hungary. Electronic address: SreterT@nebih.gov.hu.
authors.forename: T
authors.initials: T
authors.lastname: Sréter
mesh_terms: D000818:Animals; D002980:Climate; D003710:Demography; D005589:Foxes / Q000469:parasitology*; D040362:Geographic Information Systems; D006814:Hungary / Q000453:epidemiology; D007814:Larva; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000469:parasitology; D013696:Temperature; D014234:Trichinella / Q000302:isolation & purification* / Q000502:physiology; D017160:Trichinella spiralis / Q000302:isolation & purification / Q000502:physiology; D014235:Trichinellosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*
keywords: Geographic information system; Non-agricultural areas; Spatial distribution; Temperature; Trichinella britovi; Trichinella spiralis