title: A human monoclonal anti-TNF alpha antibody (adalimumab) reduces airway inflammation and ameliorates lung histology in a murine model of acute asthma.
abstract: BACKGROUND
  A few experimental studies related to asthma have unveiled the beneficial effects of TNF alpha blocking agents on the airway histology, cytokine levels in bronchoalveolar lavage and bronchial hyper-responsiveness. In the current study, we aimed to assess the effect of adalimumab on the inflammation and histology of asthma in a murine model.
  
  
  METHODS
  Twelve-week-old BALB/c (H-2d/d) female rats (n=18) were allocated into three groups, including (group I) control (phosphate-buffered saline was implemented), (group II) asthma induced with OVA (n=6), and (group III) asthma induced with OVA+treated with adalimumab (n=6). Rats were executed on the 28th day of the study. The lung samples were fixed in 10% neutral buffered formalin. Lung parenchyma, alveolus, peribronchial and perivascular inflammation were assessed. Lung pathological scoring was performed.
  
  
  RESULTS
  Severity of lung damage was found to be reduced significantly in the asthma induced with OVA+treated with adalimumab group. When compared with the untreated group, adalimumab significantly reduced the inflammatory cells around the bronchi and bronchioles, and reduced inflammation of the alveolar wall and alveolar wall thickness as well (median score=1, p=0.52). Peribronchial smooth muscle hypertrophy and oedema were significantly reduced after adalimumab administration.
  
  
  CONCLUSIONS
  Adalimumab (a human monoclonal anti-TNF alpha antibody) therapy significantly reduced the severity of lung damage by decreasing cellular infiltration and improvement on the lung histology in a murine model of acute asthma.
authors.affiliation: Department of Pediatric Allergy and Asthma, Fatih University Faculty of Medicine, Ankara, Turkey; Department of Anesthesia, Fatih University Faculty of Medicine, Ankara, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Catal
authors.affiliation: Department of Pediatric Allergy and Asthma, Fatih University Faculty of Medicine, Ankara, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Mete
authors.affiliation: Department of Pediatric Allergy and Asthma, Fatih University Faculty of Medicine, Ankara, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Tayman
authors.affiliation: Department of Pediatric Allergy and Asthma, Gazi University Faculty of Medicine, Ankara, Turkey. Electronic address: erdemtopal44@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Topal
authors.affiliation: Department of Pathology, Ankara Numune Education and Research Hospital, Ankara, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Albayrak
authors.affiliation: Department of Anesthesia, Fatih University Faculty of Medicine, Ankara, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Sert
mesh_terms: D000068879:Adalimumab; D000485:Allergens / Q000276:immunology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000009:adverse effects; D061067:Antibodies, Monoclonal, Humanized / Q000008:administration & dosage* / Q000009:adverse effects; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000188:drug therapy* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000485:Allergens; D000893:Anti-Inflammatory Agents; D061067:Antibodies, Monoclonal, Humanized; D014409:Tumor Necrosis Factor-alpha; D010047:Ovalbumin; D000068879:Adalimumab
keywords: Adalimumab; Anti-TNF alpha; Antibody; Asthma; Cytokine; Inflammation; Murine; Ovalbumin