title: Current development in genetic engineering strategies of Bacillus species.
abstract: The complete sequencing and annotation of the genomes of industrially-important Bacillus species has enhanced our understanding of their properties, and allowed advances in genetic manipulations in other Bacillus species. Post-genomic studies require simple and highly efficient tools to enable genetic manipulation. Here, we summarize the recent progress in genetic engineering strategies for Bacillus species. We review the available genetic tools that have been developed in Bacillus species, as well as methods developed in other species that may also be applicable in Bacillus. Furthermore, we address the limitations and challenges of the existing methods, and discuss the future research prospects in developing novel and useful tools for genetic modification of Bacillus species.
authors.forename: Huina
authors.initials: H
authors.lastname: Dong
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. zhang_dw@tib.cas.cn.
authors.forename: Dawei
authors.initials: D
authors.lastname: Zhang
mesh_terms: D001407:Bacillus / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering / Q000639:trends*; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D009962:Orotate Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D010430:Pentosyltransferases / Q000235:genetics / Q000378:metabolism; D011743:Pyrimidines / Q000737:chemistry / Q000378:metabolism; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011743:Pyrimidines; D012097:Repressor Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D010430:Pentosyltransferases; D009962:Orotate Phosphoribosyltransferase; C023269:uracil phosphoribosyltransferase; C045073:Cre recombinase; D019426:Integrases; C030986:pyrimidine