title: Proteomic identification of target proteins in normal but nonfertilizing sperm.
abstract: OBJECTIVE
  To identify the male molecular causes of failures of IVF (with a deficient binding of spermatozoa to the zona pellucida, without any obvious oocyte anomaly), which are undetected by classical sperm analysis.
  
  
  METHODS
  Case-control prospective study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Proteomic profiles of spermatozoa in patients with a complete failure of fertilization and no spermatozoa bound to the zona pellucida were compared with those of controls (men with normal fertilization and cleavage rates after classical IVF for tubal indication).
  
  
  METHODS
  All samples were analyzed by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) after being divided into three fractions according to their isoelectric point.
  
  
  METHODS
  Differentially expressed proteins between infertile men and controls were identified by mass spectrometry.
  
  
  RESULTS
  Seventeen proteins differentially expressed between cases and controls were found. Twelve of these proteins were identified by mass spectrometry, and two may influence gametes interaction: laminin receptor LR67 and L-xylulose reductase (P34H).
  
  
  CONCLUSIONS
  This study shows that 2D-DIGE might be useful in finding potential targets for diagnosis and prognosis of idiopathic infertility in IVF.
authors.affiliation: Department of Histology and Reproductive Biology, UMPC (Paris VI), Tenon Hospital, Assistance Publique des Hôpitaux de Paris, Paris, France. Electronic address: c.frapsauce@chu-tours.fr.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Frapsauce
authors.affiliation: Plateforme post-génomique de la Pitié-Salpêtrière, UPMC, Paris, France.
authors.forename: Cedric
authors.initials: C
authors.lastname: Pionneau
authors.affiliation: Inserm, Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Bouley
authors.affiliation: Department of Histology and Reproductive Biology, UMPC (Paris VI), Tenon Hospital, Assistance Publique des Hôpitaux de Paris, Paris, France.
authors.forename: Vanina
authors.initials: V
authors.lastname: Delarouziere
authors.affiliation: Department of Histology and Reproductive Biology, UMPC (Paris VI), Tenon Hospital, Assistance Publique des Hôpitaux de Paris, Paris, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Berthaut
authors.affiliation: Department of Histology and Reproductive Biology, UMPC (Paris VI), Tenon Hospital, Assistance Publique des Hôpitaux de Paris, Paris, France.
authors.forename: Celia
authors.initials: C
authors.lastname: Ravel
authors.affiliation: Gynaecology, Obstetrics and Reproductive Medecine, Tenon Hospital, Assistance Publique des Hôpitaux de Paris, Paris, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Antoine
authors.affiliation: Inserm, Paris, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Soubrier
authors.affiliation: Department of Histology and Reproductive Biology, UMPC (Paris VI), Tenon Hospital, Assistance Publique des Hôpitaux de Paris, Paris, France.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Mandelbaum
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D005307:Fertilization in Vitro; D006785:Hospitals, University; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism* / Q000503:physiopathology; D007526:Isoelectric Point; D008297:Male; D011446:Prospective Studies; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D017474:Receptors, Laminin / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000378:metabolism*; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D017211:Treatment Failure; D057825:Two-Dimensional Difference Gel Electrophoresis; D015044:Zona Pellucida / Q000378:metabolism
chemical_list: D015415:Biomarkers; D011506:Proteins; C510624:RPSA protein, human; D017474:Receptors, Laminin; D012269:Ribosomal Proteins; D013401:Sugar Alcohol Dehydrogenases; C023300:L-xylulose reductase
keywords: Spermatozoa; fertilization; proteome; two-dimensional electrophoresis; zona pellucida