title: Structure and biological activities of an alginate from Sargassum fusiforme, and its sulfated derivative.
abstract: An alginate fraction, 04S2P, was isolated from the brown seaweed Sargassum fusiforme and was structurally characterized by the ratio (M/G) of β-d-mannuronic acid residues (M) to α-l-guluronic acid residues (G) via (1)H and (13)C NMR spectroscopy. When compared to commercial alginate (Alg) and alginates from other brown algae, 04S2P has a higher M/G ratio of 9.0:1.0 as determined by a modified high-performance liquid chromatography method after pre-column derivatization with PMP. Furthermore, the sulfated polysaccharides 04S2P-S and Alg-S were prepared by the chlorosulfonic acid-pyridine method. Both C-2 and/or C-3 of M and G residues of 04S2P-S were substituted by sulfate groups, with C-3 of M residues preferentially substituted. Their effects on tube formation of HMEC-1 cells were examined, and the results indicated that the sulfated Alg, Alg-S, exhibited a strong anti-angiogenic effect on HMEC-1 cells. The anti-tumor activity of native and sulfated alginates was tested on five different tumor cell lines. Alg-S demonstrated significant anti-tumor effects on the Bel7402, SMMC7721, and HT-29 cell lines, whereas 04S2P-S showed a distinct anti-tumor effect only on the Bel7402 cell line.
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China.
authors.forename: Qifei
authors.initials: Q
authors.lastname: Cong
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Liao
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China. Electronic address: dongqun@simm.ac.cn.
authors.forename: Qun
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China. Electronic address: dingkan@simm.ac.cn.
authors.forename: Kan
authors.initials: K
authors.lastname: Ding
mesh_terms: D000464:Alginates / Q000737:chemistry* / Q000494:pharmacology*; D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D044647:Sargassum / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000737:chemistry*
chemical_list: D000464:Alginates; D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents; D006603:Hexuronic Acids; D013431:Sulfates; D020723:Glucuronic Acid
keywords: Alginate; Anti-angiogenic activity; Sargassum fusiforme