title: A general strategy for site-directed enzyme immobilization by using NiO nanoparticle decorated mesoporous silica.
abstract: Mesoporous materials have recently gained much attention owing to their large surface area, narrow pore size distribution, and superior pore structure. These materials have been demonstrated as excellent solid supports for immobilization of a variety of proteins and enzymes for their potential applications as biocatalysts in the chemical and pharmaceutical industries. However, the lack of efficient and reproducible methods for immobilization has limited the activity and recyclability of these biocatalysts. Furthermore, the biocatalysts are usually not robust owing to their rapid denaturation in bulk solvents. To solve these problems, we designed a novel hybrid material system, mesoporous silica immobilized with NiO nanoparticles (SBA-NiO), wherein enzyme immobilization is directed to specific sites on the pore surface of the material. This yielded the biocatalytic species with higher activity than free enzyme in solution. These biocatalytic species are recyclable with minimal loss of activity after several cycles, demonstrating an advantage over free enzymes.
authors.affiliation: Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul 151-742 (Korea); School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 (Korea).
authors.forename: Daishun
authors.initials: D
authors.lastname: Ling
authors.forename: Liqian
authors.initials: L
authors.lastname: Gao
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Wang
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Shokouhimehr
authors.forename: Jiahui
authors.initials: J
authors.lastname: Liu
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Yu
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Hackett
authors.forename: Pui-Kin
authors.initials: PK
authors.lastname: So
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Yao
authors.forename: Jiang
authors.initials: J
authors.lastname: Xia
authors.forename: Taeghwan
authors.initials: T
authors.lastname: Hyeon
mesh_terms: D000327:Adsorption; D004800:Enzymes, Immobilized / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D009569:Nitric Oxide; D012822:Silicon Dioxide
keywords: enzyme catalysis; immobilization; mesoporous materials; nanoparticles; proteins