title: Clostridium bornimense sp. nov., isolated from a mesophilic, two-phase, laboratory-scale biogas reactor.
abstract: A novel anaerobic, mesophilic, hydrogen-producing bacterium, designated strain M2/40(T), was isolated from a mesophilic, two-phase, laboratory-scale biogas reactor fed continuously with maize silage supplemented with 5% wheat straw. 16S rRNA gene sequence comparison revealed an affiliation to the genus Clostridium sensu stricto (cluster I of the clostridia), with Clostridium cellulovorans as the closest characterized species, showing 93.8% sequence similarity to the type strain. Cells of strain M2/40(T) were rods to elongated filamentous rods that showed variable Gram staining. Optimal growth occurred at 35 °C and at pH 7. Grown on glucose, the main fermentation products were H2, CO2, formate, lactate and propionate. The DNA G+C content was 29.6 mol%. The major fatty acids (>10 %) were C(16 : 0), summed feature 10 (C(18 : 1)ω11c/ω9t/ω6t and/or unknown ECL 17.834) and C(18 : 1)ω11c dimethylacetal. Based on phenotypic, chemotaxonomic and phylogenetic differences, strain M2/40(T) represents a novel species within the genus Clostridium, for which we propose the name Clostridium bornimense sp. nov. The type strain is M2/40(T) ( = DSM 25664(T) = CECT 8097(T)).
authors.affiliation: Department of Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), D-14469 Potsdam, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Hahnke
authors.affiliation: Department of Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), D-14469 Potsdam, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Striesow
authors.affiliation: Organic Geochemistry Group, MARUM-Center for Marine Environmental Sciences and Department of Geosciences, University of Bremen, D-28359 Bremen, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Elvert
authors.affiliation: Organic Geochemistry Group, MARUM-Center for Marine Environmental Sciences and Department of Geosciences, University of Bremen, D-28359 Bremen, Germany.
authors.forename: Xavier Prieto
authors.initials: XP
authors.lastname: Mollar
authors.affiliation: Department of Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), D-14469 Potsdam, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Klocke
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology*; D003013:Clostridium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005285:Fermentation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012820:Silage / Q000382:microbiology*
chemical_list: D056804:Biofuels; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S