title: Diet carotenoid lutein modulates the expression of genes related to oxygen transporters and decreases DNA damage and oxidative stress in mice.
abstract: Lutein (LT) is a carotenoid obtained by diet and despite its antioxidant activity had been biochemically reported, few studies are available concerning its influence on the expression of antioxidant genes. The expression of 84 genes implicated in antioxidant defense was quantified using quantitative reverse transcription polymerase chain reaction array. DNA damage was measured by comet assay and glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) were quantified as biochemical parameters of oxidative stress in mouse kidney and liver. cDDP treatment reduced concentration of GSH and increased TBARS, parameters that were ameliorated in treatment associated with LT. cDDP altered the expression of 32 genes, increasing the expression of GPx2, APC, Nqo1 and CCs. LT changed the expression of 37 genes with an induction of 13 mainly oxygen transporters. In treatments associating cDDP and LT, 30 genes had their expression changed with a increase of the same genes of the cDDP treatment alone. These results suggest that LT might act scavenging reactive species and also inducing the expression of genes related to a better antioxidant response, highlighting the improvement of oxygen transport. This improved redox state of the cell through LT treatment could be related to the antigenotoxic and antioxidant effects observed.
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n°, 14040-903 Ribeirão Preto, São Paulo, Brazil. Electronic address: julianaserpeloni@yahoo.com.br.
authors.forename: Juliana Mara
authors.initials: JM
authors.lastname: Serpeloni
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR (445), Km 380, 86051-990 Londrina, Paraná, Brazil.
authors.forename: Ilce Mara de Syllos
authors.initials: IM
authors.lastname: Cólus
authors.affiliation: Departamento de Cirurgia, Traumatologia Buco-Maxilo-Facial e Periodontia, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n°, 14040-903 Ribeirão Preto, São Paulo, Brazil.
authors.forename: Fabíola Singaretti
authors.initials: FS
authors.lastname: de Oliveira
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n°, 14040-903 Ribeirão Preto, São Paulo, Brazil.
authors.forename: Alexandre Ferro
authors.initials: AF
authors.lastname: Aissa
authors.affiliation: Departamento de Ciência de Alimentos, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas, Cidade Universitária "Zeferino Vaz", 13083-862 Campinas, São Paulo, Brazil.
authors.forename: Adriana Zerlotti
authors.initials: AZ
authors.lastname: Mercadante
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n°, 14040-903 Ribeirão Preto, São Paulo, Brazil.
authors.forename: Maria Lourdes Pires
authors.initials: ML
authors.lastname: Bianchi
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n°, 14040-903 Ribeirão Preto, São Paulo, Brazil.
authors.forename: Lusânia Maria Greggi
authors.initials: LM
authors.lastname: Antunes
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002945:Cisplatin / Q000009:adverse effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D005786:Gene Expression Regulation; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D014975:Lutein / Q000494:pharmacology*; D008297:Male; D051379:Mice; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010100:Oxygen / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; C491962:Ccs protein, mouse; D018832:Molecular Chaperones; D017392:Thiobarbituric Acid Reactive Substances; C492172:Gpx2 protein, mouse; D005979:Glutathione Peroxidase; D016660:NAD(P)H Dehydrogenase (Quinone); C491732:Nqo1 protein, mouse; D064173:Anaphase-Promoting Complex-Cyclosome; D005978:Glutathione; D002945:Cisplatin; D010100:Oxygen; D014975:Lutein
keywords: Cisplatin; Gene expression; Genotoxicity; Glutathione; Mice; Nutrigenomics