title: Synthesis of gold-cellobiose nanocomposites for colorimetric measurement of cellobiase activity.
abstract: Gold-cellobiose nanocomposites (GCNCs) were synthesized by reducing gold salt with a polysaccharide, cellobiose. Here, cellobiose acted as a controller of nucleation or stabilizer in the formation of gold nanoparticles. The obtained GCNCs were characterized with UV-visible spectroscopy; Zetasizer and Fourier transform infrared (FT-IR) spectrophotometer. Moreover, 6-Mercapto-1-hexanol (MCH) was modified on GCNCs, and the MCH-GCNCs were used to determine the cellobiase activity in compost extracts based on the surface plasmon resonance (SPR) property of MCH-GCNCs. The degradation of cellobiose on MCH-GCNCs by cellobiase could induce the aggregation, and the SPR absorption wavelength of MCH-GCNCs correspondingly red shifted. Thus, the absorbance ratio of treated MCH-GCNCs (A650/A520) could be used to estimate the cellobiase activity, and the probe exhibited highly sensitive and selective detection of the cellobiase activity with a wide linear from 3.0 to 100.0U L(-1) within 20 min. Meanwhile, a good linear relationship with correlation coefficient of R2=0.9976 was obtained. This approach successfully showed the suitability of gold nanocomposites as a colorimetric sensor for the sensitive and specific enzyme activity detection.
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China.
authors.forename: Cui
authors.initials: C
authors.lastname: Lai
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China. Electronic address: zgming@hnu.edu.cn.
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Zeng
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China. Electronic address: huangdanlian1981@163.com.
authors.forename: Dan-Lian
authors.initials: DL
authors.lastname: Huang
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China.
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Zhao
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wei
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China.
authors.forename: Piao
authors.initials: P
authors.lastname: Xu
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China.
authors.forename: Ning-Jie
authors.initials: NJ
authors.lastname: Li
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, Hunan, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China.
authors.forename: Mingyong
authors.initials: M
authors.lastname: Lai
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, Hunan, PR China.
authors.forename: Yibin
authors.initials: Y
authors.lastname: He
mesh_terms: D002475:Cellobiose / Q000378:metabolism*; D003124:Colorimetry / Q000379:methods*; D057075:Enzyme Assays; D006046:Gold / Q000737:chemistry*; D000441:Hexanols / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry*; D010316:Particle Size; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013438:Sulfhydryl Compounds / Q000737:chemistry; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: C503488:6-mercapto-1-hexanol; D000441:Hexanols; D012996:Solutions; D013438:Sulfhydryl Compounds; D002475:Cellobiose; D006046:Gold; D001617:beta-Glucosidase
keywords: Cellobiase activity; Colorimetric; Determination; Gold–cellobiose nanocomposites; Sensitivity