title: Screening and monitoring zearalenone-producing Fusarium species by PCR and zearalenone by monoclonal antibodies in feed from China.
abstract: Screening of zearalenone (ZEN)-producing species and monitoring of ZEN in feed were performed by using anti-zearalenone monoclonal antibodies. ELISA recoveries of ZEN from corn distillers dried grains with solubles (DDGS) feed, corn feed, rice bran, soybean meal, wheat bran and rapeseed dregs were between 78.6% and 88.6%. ZEN recovery from culture media was 96.3% at the spiked level of 500 µg/kg. Eighty-three samples of DDGS feed, corn feed and other fee ingredients were collected from 11 provinces of China and analysed for ZEN. Average ZEN levels were 563.4 µg/kg for DDGS feed, 393.1 µg/kg for corn feed and 65.7 µg/kg for other feed ingredients. Eighteen Fusarium species such as Fusarium graminearum which could produce ZEN were isolated from corn feed and other feed ingredients.
authors.affiliation: a State Key Laboratory of Animal Nutrition , Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing , P.R. China.
authors.forename: Shi-Chun
authors.initials: SC
authors.lastname: Pei
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Zhen
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Gao
authors.forename: Won-Jong
authors.initials: WJ
authors.lastname: Lee
authors.forename: Hong-Fu
authors.initials: HF
authors.lastname: Zhang
authors.forename: Cheng
authors.initials: C
authors.lastname: Ji
authors.forename: Xuan-Zhe
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Cong
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002681:China; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005670:Fusarium / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016133:Polymerase Chain Reaction; D003313:Zea mays / Q000382:microbiology*; D015025:Zearalenone / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D015025:Zearalenone
keywords: China; ELISA; Fusarium; PCR; feed; zearalenone