title: CTSB promotes porcine preadipocytes differentiation by degrading fibronectin and attenuating the Wnt/β-catenin signaling pathway.
abstract: The process of preadipocytes differentiation plays a vital role in adipose tissue expansion and many factors are involved in this event. Cathepsin B (CTSB), secreted from lysosome, has been reported in regulating a variety of physiological processes. In this study, we demonstrated CTSB promotes lipid accumulation and adipogenic genes expression in porcine primary preadipocytes by degrading fibronectin (Fn), a key component of extracellular matrix. Lithium chloride (LiCl) is an activator of Wnt/β-catenin signaling through stabilizing β-catenin. We found that CTSB can relieve the anti-adipogenic effects of LiCl, indicating that CTSB could impact Wnt/β-catenin signaling pathway. Interestingly, Fn is an important target gene of Wnt/β-catenin. So we considered that CTSB promote preadipocytes differentiation by suppressing these two pathways.
authors.affiliation: Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling, 712100, People's Republic of China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Yin
authors.initials: Y
authors.lastname: Mai
authors.forename: Hao
authors.initials: H
authors.lastname: Yang
authors.forename: Pei-Yue
authors.initials: PY
authors.lastname: Dong
authors.forename: Xue-Li
authors.initials: XL
authors.lastname: Zheng
authors.forename: Gong-She
authors.initials: GS
authors.lastname: Yang
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D000818:Animals; D002401:Cathepsin B / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D018021:Lithium Chloride / Q000494:pharmacology*; D013552:Swine; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: D005353:Fibronectins; D002401:Cathepsin B; D018021:Lithium Chloride