title: Plasmodium falciparum founder populations in western Cambodia have reduced artemisinin sensitivity in vitro.
abstract: Reduced Plasmodium falciparum sensitivity to short-course artemisinin (ART) monotherapy manifests as a long parasite clearance half-life. We recently defined three parasite founder populations with long half-lives in Pursat, western Cambodia, where reduced ART sensitivity is prevalent. Using the ring-stage survival assay, we show that these founder populations have reduced ART sensitivity in vitro at the early ring stage of parasite development and that a genetically admixed population contains subsets of parasites with normal or reduced ART sensitivity.
authors.affiliation: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Chanaki
authors.initials: C
authors.lastname: Amaratunga
authors.affiliation: Malaria Molecular Epidemiology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
authors.forename: Benoit
authors.initials: B
authors.lastname: Witkowski
authors.affiliation: National Center for Parasitology, Entomology, and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Dalin
authors.initials: D
authors.lastname: Dek
authors.affiliation: National Center for Parasitology, Entomology, and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Vorleak
authors.initials: V
authors.lastname: Try
authors.affiliation: Malaria Molecular Epidemiology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
authors.forename: Nimol
authors.initials: N
authors.lastname: Khim
authors.affiliation: MRC Centre for Genomics and Global Health, University of Oxford, Oxford, United Kingdom Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand Wellcome Trust Sanger Institute, Cambridge, United Kingdom.
authors.forename: Olivo
authors.initials: O
authors.lastname: Miotto
authors.affiliation: Malaria Molecular Epidemiology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia dmenard@pasteur-kh.org rfairhurst@niaid.nih.gov.
authors.forename: Didier
authors.initials: D
authors.lastname: Ménard
authors.affiliation: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA dmenard@pasteur-kh.org rfairhurst@niaid.nih.gov.
authors.forename: Rick M
authors.initials: RM
authors.lastname: Fairhurst
mesh_terms: D000483:Alleles; D000962:Antimalarials / Q000494:pharmacology*; D037621:Artemisinins / Q000494:pharmacology*; D002160:Cambodia / Q000453:epidemiology; D004351:Drug Resistance / Q000235:genetics*; D018703:Founder Effect; D006207:Half-Life; D006801:Humans; D008018:Life Cycle Stages / Q000187:drug effects* / Q000235:genetics; D016778:Malaria, Falciparum / Q000188:drug therapy / Q000453:epidemiology / Q000469:parasitology; D009154:Mutation; D021261:Parasitic Sensitivity Tests; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development
chemical_list: D000962:Antimalarials; D037621:Artemisinins; C031327:artemisinin