title: Fabrication, characterization, and biocompatibility of ethyl cellulose/carbonated hydroxyapatite composite coatings on Ti6Al4V.
abstract: In order to improve the biocompatibility of metallic implants, bioactive components are often used as coatings so that a real bond with the surrounding bone tissue can be formed. We prepared ethyl cellulose/carbonated hydroxyapatite composite coatings (ECHCs) on Ti6Al4V substrates with carbonated hydroxyapatite coatings (CHACs) without ethyl cellulose as controls. The inorganic constituent on the CHACs and ECHCs is calcium-deficient carbonated hydroxyapatite with a flaky texture and a low degree of crystallinity. The flaky carbonated hydroxyapatite plates aggregate to form macropores with an aperture size of around 0.5-2.0 μm. The presence of ethyl cellulose provides superior morphology, contact angle, and biocompatibility characteristics. In comparison to CHACs, ECHCs exhibit a smoother, crack-free surface because the cracks are filled by ethyl cellulose. Moreover, the contact angle of ECHCs is 37.3°, greater than that of CHACs (13.0°). Surface biocompatibility was investigated by using human bone mesenchymal stem cells (hBMSCs). The attachment, spreadability, viability and proliferation of hBMSCs on ECHCs are superior to those on CHACs. Thus, the crack-free ECHCs have excellent biocompatibility and are appropriate for use as biological implants.
authors.affiliation: Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, 200011, Shanghai, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tian
authors.forename: Sha
authors.initials: S
authors.lastname: Tang
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Teng
authors.initials: T
authors.lastname: Long
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Qu
authors.forename: De-Gang
authors.initials: DG
authors.lastname: Yu
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Guo
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Guo
authors.forename: Zhen-An
authors.initials: ZA
authors.lastname: Zhu
mesh_terms: D000497:Alloys; D001672:Biocompatible Materials; D002254:Carbonates / Q000737:chemistry*; D002478:Cells, Cultured; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D017886:Durapatite; D006801:Humans; D008855:Microscopy, Electron, Scanning; D033362:Powder Diffraction; D014025:Titanium
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D002254:Carbonates; C031462:titanium alloy (TiAl6V4); C013517:ethyl cellulose; D002482:Cellulose; D017886:Durapatite; D014025:Titanium