title: Watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) juice modulates oxidative damage induced by low dose X-ray in mice.
abstract: Watermelon is a natural product that contains high level of antioxidants and may prevent oxidative damage in tissues due to free radical generation following an exposure to ionizing radiation. The present study aimed to investigate the radioprotective effects of watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) juice against oxidative damage induced by low dose X-ray exposure in mice. Twelve adult male ICR mice were randomly divided into two groups consisting of radiation (Rx) and supplementation (Tx) groups. Rx received filtered tap water, while Tx was supplemented with 50% (v/v) watermelon juice for 28 days ad libitum prior to total body irradiation by 100 μGy X-ray on day 29. Brain, lung, and liver tissues were assessed for the levels of malondialdehyde (MDA), apurinic/apyrimidinic (AP) sites, glutathione (GSH), and superoxide dismutase (SOD) inhibition activities. Results showed significant reduction of MDA levels and AP sites formation of Tx compared to Rx (P < 0.05). Mice supplemented with 50% watermelon juice restore the intracellular antioxidant activities by significantly increased SOD inhibition activities and GSH levels compared to Rx. These findings may postulate that supplementation of 50% watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) juice could modulate oxidative damage induced by low dose X-ray exposure.
authors.affiliation: Department of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, UiTM Puncak Alam, 42300 Bandar Puncak Alam, Selangor, Malaysia.
authors.forename: Mohd Khairul Amran
authors.initials: MK
authors.lastname: Mohammad
authors.affiliation: Department of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, UiTM Puncak Alam, 42300 Bandar Puncak Alam, Selangor, Malaysia.
authors.forename: Muhamad Idham
authors.initials: MI
authors.lastname: Mohamed
authors.affiliation: Department of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, UiTM Puncak Alam, 42300 Bandar Puncak Alam, Selangor, Malaysia.
authors.forename: Ainul Mardhiyah
authors.identifier: 0000-0001-8710-0685
authors.initials: AM
authors.lastname: Zakaria
authors.affiliation: Department of Medical Imaging, Faculty of Health Sciences, Universiti Teknologi MARA, UiTM Puncak Alam, 42300 Bandar Puncak Alam, Selangor, Selangor, Malaysia.
authors.forename: Hairil Rashmizal
authors.initials: HR
authors.lastname: Abdul Razak
authors.affiliation: Department of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, UiTM Puncak Alam, 42300 Bandar Puncak Alam, Selangor, Malaysia.
authors.forename: Wan Mazlina Md
authors.initials: WM
authors.lastname: Saad
mesh_terms: D000818:Animals; D029773:Citrullus / Q000737:chemistry*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009928:Organ Specificity / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000188:drug therapy* / Q000378:metabolism*; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D014965:X-Rays
chemical_list: D011837:Radiation-Protective Agents