title: Nanovesicle-based platform for the electrophysiological monitoring of aquaporin-4 and the real-time detection of its antibody.
abstract: Aquaporin-4 (AQP4) water channel protein transports water molecules across cell membranes bidirectionally and involves in a neurological disorder, neuromyelitis optica (NMO) caused by anti-AQP4 antibodies. Here, we developed a platform based on nanovesicle-carbon nanotube hybrid nanostructures for the real-time detection of anti-AQP4 antibodies and the electrophysiological monitoring of AQP4 activities. Using the hybrid device, we could detect anti-AQP4 antibodies with a high sensitivity and estimate the binding constants under different osmotic conditions. The results show AQP4 had a better affinity to anti-AQP4 antibodies under hyper-osmotic conditions than normal conditions. Furthermore, our device can be utilized to study the real-time cellular responses related with AQP4 such as those to different osmotic stresses. This nanovesicle-based platform can be a simple but versatile tool for basic research about AQP4 and related biomedical applications such as disease diagnostics.
authors.affiliation: Department of Physics and Astronomy, and Institute of Applied Physics, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Park
authors.affiliation: Department of Physics and Astronomy, and Institute of Applied Physics, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Juhun
authors.initials: J
authors.lastname: Park
authors.affiliation: Sensor System Research Center, Korea Institute of Science and Technology, Seoul 151-742, Republic of Korea.
authors.forename: Hyun Seok
authors.initials: HS
authors.lastname: Song
authors.affiliation: Department of Physiology, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea.
authors.forename: Sung Joon
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea.
authors.forename: Kyeong Cheon
authors.initials: KC
authors.lastname: Jung
authors.affiliation: Department of Neurology, Seoul National University Hospital, Seoul 110-744, Republic of Korea.
authors.forename: Sung-Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Department of Physics and Astronomy, and Institute of Applied Physics, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Dong-guk
authors.initials: DG
authors.lastname: Cho
authors.affiliation: Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Daesan
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Neurology, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea. Electronic address: pks1126@chol.com.
authors.forename: Kyung Seok
authors.initials: KS
authors.lastname: Park
authors.affiliation: Department of Physics and Astronomy, and Institute of Applied Physics, Seoul National University, Seoul 151-747, Republic of Korea; Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151-747, Republic of Korea. Electronic address: seunghun@snu.ac.kr.
authors.forename: Seunghun
authors.initials: S
authors.lastname: Hong
mesh_terms: D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D051401:Aquaporin 4 / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation*; D002462:Cell Membrane; D055724:Electrophysiological Phenomena; D004867:Equipment Design; D057809:HEK293 Cells; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009471:Neuromyelitis Optica / Q000276:immunology; D009997:Osmotic Pressure
chemical_list: D000906:Antibodies; D051401:Aquaporin 4; D037742:Nanotubes, Carbon
keywords: Anti-aquaporin-4 antibody; Aquaporin-4; Neuromyelitis optica; Osmotic stress