title: Determination of domperidone in human plasma using high performance liquid chromatography with fluorescence detection for clinical application.
abstract: A simple and reliable method for the determination of domperidone in human plasma has been developed. Plasma samples (1mL) were pre-purified by a solid-phase extraction with Bond Elut(®) C18. The separation was achieved with XBridge™ C18 column (150mm×4.6mm i.d., 5μm) at 40°C. The mobile phase was a mixture of acetonitrile and 10mM ammonium acetate buffer (36:64, v/v), adjusted to pH 9.4 with 20% ammonium solution at a flow rate of 1.0mL/min. The peak was detected using fluorescence detector at excitation 282nm and emission 328nm. Retention times for domperidone and internal standard (propranolol) were 8.3min and 11.2min, respectively. The method showed a good linearity (r>0.999), precision (relative standard deviations <10.6%), and extraction recovery (85.7-99.7%) over a concentration of 1-100ng/mL. The lower limit of quantification (LLOQ) was 1.0ng/mL. This proposed method was successfully applied to a pharmacokinetic interaction study of domperidone in healthy Japanese volunteers.
authors.affiliation: Department of Pharmaceutical Medicine and Communication, Oita University Faculty of Medicine, Hasama-machi, Yufu-shi, Oita 879-5593, Japan. Electronic address: t-yoshi@oita-u.ac.jp.
authors.forename: Tsuneaki
authors.initials: T
authors.lastname: Yoshizato
authors.affiliation: Department of Pharmaceutical Medicine and Communication, Oita University Faculty of Medicine, Hasama-machi, Yufu-shi, Oita 879-5593, Japan. Electronic address: kotek@oita-u.ac.jp.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Tsutsumi
authors.affiliation: Department of Clinical Pharmacology and Therapeutics, Oita University Faculty of Medicine, Hasama-machi, Yufu-shi, Oita 879-5593, Japan. Electronic address: kotet@oita-u.ac.jp.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kotegawa
authors.affiliation: Department of Clinical Pharmacology and Therapeutics, Oita University Faculty of Medicine, Hasama-machi, Yufu-shi, Oita 879-5593, Japan. Electronic address: imaih@oita-u.ac.jp.
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Imai
authors.affiliation: Department of Pharmaceutical Medicine and Communication, Oita University Faculty of Medicine, Hasama-machi, Yufu-shi, Oita 879-5593, Japan. Electronic address: snakano@oita-u.ac.jp.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Nakano
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004294:Domperidone / Q000097:blood* / Q000493:pharmacokinetics; D005453:Fluorescence; D006801:Humans; D017964:Itraconazole / Q000493:pharmacokinetics; D057230:Limit of Detection; D008297:Male; D015203:Reproducibility of Results; D052616:Solid Phase Extraction
chemical_list: D017964:Itraconazole; D004294:Domperidone
keywords: Domperidone; Fluorescence detection; HPLC; Itraconazole; MDR1; Solid phase extraction