title: Endogenous antibodies for tumor detection.
abstract: The study of cancer immunology has provided diagnostic and therapeutic instruments through serum autoantibody biomarkers and exogenous monoclonal antibodies. While some endogenous antibodies are found within or surrounding transformed tissue, the extent to which this exists has not been entirely characterized. We find that in transgenic and xenograft mouse models of cancer, endogenous gamma immunoglobulin (IgG) is present at higher concentration in malignantly transformed organs compared to non-transformed organs in the same mouse or organs of cognate wild-type mice. The enrichment of endogenous antibodies within the malignant tissue provides a potential means of identifying and tracking malignant cells in vivo as they mutate and diversify. Exploiting these antibodies for diagnostic and therapeutic purposes is possible through the use of agents that bind endogenous antibodies.
authors.affiliation: 1] Laboratory of Cellular Biophysics, The Rockefeller University, New York, NY 10065 [2] Division of Pediatric Surgery, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY 10065 [3].
authors.forename: Barrie S
authors.initials: BS
authors.lastname: Rich
authors.affiliation: 1] Laboratory of Cellular Biophysics, The Rockefeller University, New York, NY 10065 [2] Division of Pediatric Surgery, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY 10065 [3].
authors.forename: Joshua N
authors.initials: JN
authors.lastname: Honeyman
authors.affiliation: 1] Laboratory of Cellular Biophysics, The Rockefeller University, New York, NY 10065 [2] Division of Pediatric Surgery, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY 10065.
authors.forename: David G
authors.initials: DG
authors.lastname: Darcy
authors.affiliation: Laboratory of Cellular Biophysics, The Rockefeller University, New York, NY 10065.
authors.forename: Peter T
authors.initials: PT
authors.lastname: Smith
authors.affiliation: Laboratory of Cellular Biophysics, The Rockefeller University, New York, NY 10065.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Williams
authors.affiliation: 1] Laboratory of Cellular Biophysics, The Rockefeller University, New York, NY 10065 [2] Division of Pediatric Surgery, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY 10065.
authors.forename: Irene Isabel P
authors.initials: II
authors.lastname: Lim
authors.affiliation: Center of Comparative Medicine and Pathology, Laboratory of Comparative Pathology, Memorial Sloan-Kettering Cancer Center, New York, NY 10065.
authors.forename: Linda K
authors.initials: LK
authors.lastname: Johnson
authors.affiliation: Department of Epidemiology and Biostatistics, Memorial Sloan-Kettering Cancer Center, New York, NY 10065.
authors.forename: Mithat
authors.initials: M
authors.lastname: Gönen
authors.affiliation: The Dalton School, New York, NY 10128.
authors.forename: Joel S
authors.initials: JS
authors.lastname: Simon
authors.affiliation: Division of Pediatric Surgery, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY 10065.
authors.forename: Michael P
authors.initials: MP
authors.lastname: LaQuaglia
authors.affiliation: Laboratory of Cellular Biophysics, The Rockefeller University, New York, NY 10065.
authors.forename: Sanford M
authors.initials: SM
authors.lastname: Simon
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor / Q000276:immunology*; D045744:Cell Line, Tumor; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D007074:Immunoglobulin G