title: Oral and intraperitoneal administration of quercetin decreased lymphocyte DNA damage and plasma lipid peroxidation induced by TSA in vivo.
abstract: Our previous study showed that quercetin enhances the anticancer effect of trichostatin A (TSA) in xenograft mice given quercetin intraperitoneally (10 mg/kg, 3 times/week). Herein, we investigate whether quercetin administered orally exerts such an effect and prevents the cytotoxic side effects of TSA. We found that quercetin given orally (20 and 100 mg/kg, 3 times/week) failed to enhance the antitumor effect of TSA although it increased the total quercetin concentration more than quercetin administered intraperitoneally in the plasma. The compound quercetin-3-glucuronide (Q3G) increased the most. However, quercetin administered intraperitoneally increased the total quercetin level in tumor tissues more than oral quercetin. Oral and intraperitoneal administration of quercetin similarly decreased lymphocyte DNA damage and plasma lipid peroxidation level induced by TSA. Furthermore, we found that the enhancing effect of Q3G on the antitumor effect of TSA and the incorporation of Q3G was less than that of quercetin in A549 cells. However, we found that A549 cells possessed the ability to convert Q3G to quercetin. In conclusion, different from quercetin administered intraperitoneally, quercetin administered orally failed to enhance the antitumor effect of TSA because of its metabolic conversion. However, it prevented TSA-induced DNA damage and lipid peroxidation.
authors.affiliation: Department of Nutritional Science, Chung Shan Medical University, No. 110 Section Jianguo North Road, Taichung 40201, Taiwan.
authors.forename: Shu-Ting
authors.initials: ST
authors.lastname: Chan
authors.affiliation: Department of Nutritional Science, Chung Shan Medical University, No. 110 Section Jianguo North Road, Taichung 40201, Taiwan.
authors.forename: Yi-Chin
authors.identifier: 0000-0003-4139-1354
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Department of Nutrition, Hungkuang University, No. 34 Chung Chie Road, Shalu, Taichung County 43302, Taiwan.
authors.forename: Cheng-Hung
authors.identifier: 0000-0003-1963-1825
authors.initials: CH
authors.lastname: Chuang
authors.affiliation: Department of Beauty Science, Chienkuo Technology University, Changhua 50094, Taiwan.
authors.forename: Rong-Jen
authors.initials: RJ
authors.lastname: Shiau
authors.affiliation: Graduate Institute of Veterinary Pathology, College of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan.
authors.forename: Jiunn-Wang
authors.initials: JW
authors.lastname: Liao
authors.affiliation: Department of Nutritional Science, Chung Shan Medical University, No. 110 Section Jianguo North Road, Taichung 40201, Taiwan ; Department of Nutrition, Chung Shan Medical University Hospital, Taichung 40201, Taiwan.
authors.forename: Shu-Lan
authors.initials: SL
authors.lastname: Yeh
mesh_terms: D000284:Administration, Oral; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004249:DNA Damage; D006801:Humans; D006877:Hydroxamic Acids / Q000009:adverse effects*; D007263:Infusions, Parenteral; D015227:Lipid Peroxidation; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000628:therapy*; D011794:Quercetin / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D006877:Hydroxamic Acids; C443401:quercetin 3-O-glucuronide; C012589:trichostatin A; D011794:Quercetin