title: Human leukocyte antigen-G (HLA-G) polymorphism and expression in breast cancer patients.
abstract: Human leukocyte antigen-G (HLA-G) is known to be implicated in a tumor-driven immune escape mechanism in malignancies. The purpose of this study was to investigate HLA-G polymorphism and expression in breast cancer. HLA-G alleles were determined by direct DNA sequencing procedures from blood samples of 80 breast cancer patients and 80 healthy controls. Soluble HLA-G (sHLA-G) was measured by enzyme-linked immunosorbent assay (ELISA) from serum specimens. HLA-G expression in breast cancer lesions was also analyzed by immunohistochemistry staining. The presence of HLA-G 3' untranslated region (UTR) 14-bp sequence was analyzed and found to be associated with reduced risk of breast cancer susceptibility based on HLA-G expression in tissues (P = 0.0407). Levels of sHLA-G were higher in the breast cancer group (median 117.2 U/mL) compared to the control group (median 10.1 U/mL, P<0.001). The area under the receiver operating characteristic curve (AU-ROC) values of sHLA-G for differentiating breast cancer from normal controls and for detecting metastasis from other stages of breast cancer were 0.89 and 0.79, respectively. HLA-G polymorphism and expression may be involved in breast carcinogenesis and sHLA-G concentrations could be used as a diagnostic marker for detecting breast cancer.
authors.affiliation: Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Seri
authors.initials: S
authors.lastname: Jeong
authors.affiliation: Department of Surgery, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Seho
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Surgery, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Byeong-Woo
authors.initials: BW
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang, Republic of Korea.
authors.forename: Younhee
authors.initials: Y
authors.lastname: Park
authors.affiliation: College of Animal Bioscience & Technology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Oh-Joong
authors.initials: OJ
authors.lastname: Kwon
authors.affiliation: Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Hyon-Suk
authors.initials: HS
authors.lastname: Kim
mesh_terms: D019540:Area Under Curve; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D059951:HLA-G Antigens / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D019139:Tumor Escape / Q000235:genetics*
chemical_list: D017931:DNA Primers; D059951:HLA-G Antigens