title: A novel functional conducting polymer as an immobilization platform.
abstract: Here, we present the fabrication of conducting polymer based enzymatic and microbial biosensors. To obtain immobilization platforms for both pyranose oxidase (PyOx) and Gluconobacter oxydans, the graphite electrode surface was modified with the polymer of 4-amino-N-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzamide (HKCN) which has free amino groups on the surface for further bioconjugation reactions with the biomolecules. Initially, the electrode surface was covered with HKCN via electropolymerization. Then, either PyOx or G. oxydans cell was stabilized using glutaraldehyde as a cross-linker. After optimization of biosensors, analytical characterization and surface imaging studies were investigated. The change of current depends on glucose concentration between 0.05-1.0mM and 0.25-2.5mM with HKCN/PyOx and HKCN/G. oxydans biosensors in batch systems. Also, the calibration graphs were obtained for glucose in FIA mode, and in this case, linear ranges were found to be 0.01-1.0mM and 0.1-7.5mM for HKCN/PyOx and HKCN/G. oxydans, respectively.
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova, Izmir, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Guler
authors.affiliation: Adnan Menderes University, Faculty of Art and Science, Chemistry Department, Aydin, Turkey.
authors.forename: Hakan Can
authors.initials: HC
authors.lastname: Soyleyici
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova, Izmir, Turkey. Electronic address: dilek.odaci@ege.edu.tr.
authors.forename: Dilek Odaci
authors.initials: DO
authors.lastname: Demirkol
authors.affiliation: Pamukkale University, Faculty of Art and Science, Chemistry Department, Denizli, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Ak
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova, Izmir, Turkey. Electronic address: suna.timur@ege.edu.tr.
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
mesh_terms: D001549:Benzamides / Q000737:chemistry; D015374:Biosensing Techniques; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D020600:Gluconobacter oxydans / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005976:Glutaral / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D001549:Benzamides; D004800:Enzymes, Immobilized; D011108:Polymers; C037689:benzamide; D006108:Graphite; D002237:Carbohydrate Dehydrogenases; C022489:pyranose oxidase; D005947:Glucose; D005976:Glutaral
keywords: Biosensing; Conducting polymers; G. oxydans; Pyranose oxidase