title: Light-mediated control of gene expression in filamentous fungus Trichoderma reesei.
abstract: We developed a light-mediated system based on synthetic light-switchable transactivators. The transactivators bind promoter upon blue-light exposure and rapidly initiate transcription of target transgenes in filamentous fungus Trichoderma reesei. Light is inexpensive to apply, easily delivered, and instantly removed, and thus has significant advantages over chemical inducers.
authors.affiliation: State key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China. Electronic address: wadexp@ecust.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: State key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Shi
authors.affiliation: State key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China. Electronic address: dzhwei@ecust.edu.cn.
authors.forename: Dong-Zhi
authors.initials: DZ
authors.lastname: Wei
mesh_terms: D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects*; D023061:Gene Order; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D008027:Light; D015534:Trans-Activators / Q000378:metabolism; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D015534:Trans-Activators
keywords: Light-mediated system; Light-switchable transactivator; Trichoderma reesei; Vivid