title: Development and evaluation of baculovirus-expressed Chikungunya virus E1 envelope proteins for serodiagnosis of Chikungunya infection.
abstract: Population-based serosurveillance studies provide critical estimates on community-level immunity and the potential for future outbreaks. Currently, serological assays, such as IgG enzyme-linked immunosorbent assays (ELISAs) and indirect immunofluorescence tests (IIFT) based on the inactivated whole virus are used to determine past Chikungunya virus (CHIKV) infection. However, these commercially available tests have variable sensitivities. To develop and evaluate recombinant based CHIKV-specific IgG antibody capture ELISAs (GAC-ELISAs), baculoviruses carrying wild-type (E1-A226, named WT) or mutant (E1-A226V, named MUT) E1 envelope protein genes of CHIKV were generated. The seroreactivity of recombinant CHIKV WT and MUT envelope proteins were determined using residual blood, collected from CHIKV-confirmed patients. The sensitivities of both recombinant CHIKV envelope proteins were 83.0% as measured by GAC-ELISAs. The specificities of both recombinant proteins were 87.8%. These GAC-ELISAs were also able to detect the persistence of anti-CHIKV IgG antibodies up to 6 months after the disease onset, together with rise in sensitivities with increasing time. These results suggest that the baculovirus purified recombinant CHIKV envelope proteins react with anti-CHIKV IgG antibodies and may be useful in population-based seroprevalence surveys. In addition, these GAC-ELISAs offer good diagnostic value to determine the recent/past CHIKV infection status in non-endemic populations.
authors.affiliation: Environmental Health Institute, National Environment Agency, Singapore. Electronic address: panky8@yahoo.com.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Environmental Health Institute, National Environment Agency, Singapore.
authors.forename: Kwoon-Yong
authors.initials: KY
authors.lastname: Pok
authors.affiliation: Environmental Health Institute, National Environment Agency, Singapore.
authors.forename: Li-Kiang
authors.initials: LK
authors.lastname: Tan
authors.affiliation: Communicable Disease Centre, Institute of Infectious Disease and Epidemiology, Tan Tock Seng Hospital, Singapore.
authors.forename: Chow
authors.initials: C
authors.lastname: Angela
authors.affiliation: Communicable Disease Centre, Institute of Infectious Disease and Epidemiology, Tan Tock Seng Hospital, Singapore.
authors.forename: Yee-Sin
authors.initials: YS
authors.lastname: Leo
authors.affiliation: Environmental Health Institute, National Environment Agency, Singapore.
authors.forename: Lee-Ching
authors.initials: LC
authors.lastname: Ng
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics; D065632:Chikungunya Fever / Q000175:diagnosis*; D002646:Chikungunya virus / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins
keywords: Baculovirus; Captured GAC-ELISAs; Chikungunya virus; E1 envelope protein; Mutant (MUT); Wild-type (WT)