title: Two ellagic acids isolated from roots of Sanguisorba officinalis L. promote hematopoietic progenitor cell proliferation and megakaryocyte differentiation.
abstract: Using a bioassay-directed chromatographic separation, two ellagic acids were obtained from the ethyl acetate extract of the roots of Sanguisorba officinalis L. On the basis of chemical and spectroscopic methods, the two ellagic acids were identified as 3,3',4-tri-O-methylellagic acid-4'-O-β-d-xyloside and 3,3',4-tri-O-methylellagic acid. Stimulation of cell proliferation was assayed in hematopoietic progenitor cells using the Cell Counting kit-8 method. The megakaryocyte differentiation was determined in human erythroleukemia (HEL) cells using Giemsa staining and flow cytometry analysis. The ellagic acids significantly stimulated the proliferation of Baf3/Mpl cells. Morphology analysis and megakaryocyte specific-marker CD41 staining confirmed that the ellagic acids induced megakaryocyte differentiation in HEL cells. This is the first time that 3,3',4-tri-O-methylellagic acid or 3,3',4-tri-O-methylellagic acid-4'-O-β-d-xyloside are reported to induce megakaryopoiesis, suggesting a class of small molecules which differ from others non-peptidyl, and appears to have potential for clinical development as a therapeutic agent for patients with blood platelet disorders.
authors.affiliation: College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 86611-137, China. gxplww2003@aliyun.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 86611-137, China. wjmbj@126.com.
authors.forename: Jianming
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 86611-137, China. zouwenjun@cdutcm.edu.cn.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zou
authors.affiliation: College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 86611-137, China. aiyanping198909@163.com.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Dai
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape; D004610:Ellagic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008533:Megakaryocytes / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008968:Molecular Conformation; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D031993:Sanguisorba / Q000737:chemistry*
chemical_list: C000594490:3,3',4-tri-O-methylellagic acid-4'-O-beta-D-xyloside; D006027:Glycosides; D010936:Plant Extracts; C052418:3,7,8-tri-O-methylellagic acid; D004610:Ellagic Acid