title: Effects of small intestinal submucosa content on the adhesion and proliferation of retinal pigment epithelial cells on SIS-PLGA films.
abstract: The retinal pigment epithelium (RPE) plays a critical role in the maintenance of the normal functions of the retina, particularly the photoreceptors. RPE dysfunction, vision loss and degeneration have been implicated as the cause of many retinal diseases, including retinitis pigmentosa and age-related macular degeneration (AMD). To overcome such disorders, tissue engineering could offer useful strategies, using biodegradable polymeric films to replace diseased or lost RPE. Synthetic/natural hybrid films have been studied as a temporary substrate for growing RPEs in biological implantations. In this study, we prepared small intestinal submucosa (SIS)-poly(lactic-co-glycolic) (PLGA) hybrid films and seeded human RPE cells (ARPE-19 cells) onto the film surface. We investigated the film suitability for RPE cell proliferation by MTT assay. The morphology of cellular adhesion on the film was confirmed by scanning electron microscopy (SEM). Reverse transcription-polymerase chain reaction (RT-PCR) and 3-amino-9-ethylcarbazole (AEC) staining were performed to examine mRNA expression and to compare cell proliferation on the films, using cytokeratin as a marker of RPE. Conclusively, we confirmed the higher cell survival rate and much stronger phenotype expression of RPEs on SIS-PLGA films compared to pure PLGA films. These results demonstrated the potential application of SIS-PLGA films in tissue-engineering strategies. Copyright © 2014 John Wiley & Sons, Ltd.
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nano Science and Technology and Polymer Fusion Research Centre, Chonbuk National University, Jeonju, Korea.
authors.forename: Ga Young
authors.initials: GY
authors.lastname: Lee
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nano Science and Technology and Polymer Fusion Research Centre, Chonbuk National University, Jeonju, Korea.
authors.forename: Su Ji
authors.initials: SJ
authors.lastname: Kang
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nano Science and Technology and Polymer Fusion Research Centre, Chonbuk National University, Jeonju, Korea.
authors.forename: So Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nano Science and Technology and Polymer Fusion Research Centre, Chonbuk National University, Jeonju, Korea.
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Song
authors.affiliation: Department of Ophthalmology and Visual Science, College of Medicine, Catholic University, Seoul, Korea.
authors.forename: Choun-Ki
authors.initials: CK
authors.lastname: Joo
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nano Science and Technology and Polymer Fusion Research Centre, Chonbuk National University, Jeonju, Korea.
authors.forename: Dongwon
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nano Science and Technology and Polymer Fusion Research Centre, Chonbuk National University, Jeonju, Korea.
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D007421:Intestine, Small / Q000166:cytology*; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D044968:Regenerative Medicine / Q000295:instrumentation* / Q000379:methods; D055213:Retinal Pigment Epithelium / Q000166:cytology*; D013718:Tensile Strength; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: PLGA; hybrid film; regeneration; retinal pigment epithelium; small intestinal submucosa; tissue engineering