title: Three commercial antibodies against α1-adrenergic receptor subtypes lack specificity in paraffin-embedded sections of murine tissues.
abstract: We tested the specificity of three commercially available antibodies (AB) against individual α1-adrenergic receptor subtypes (α1-ARST). We used these ABs to localize the α1-ARST proteins by immunohistochemistry in paraffin-embedded murine tissues. The specificity of the ABs was tested by comparing staining patterns in tissues from wild-type mice with those in corresponding tissues from mice with gene-targeted disruption of the respective α1-ARST, one of the most rigorous negative controls. None of the tested ABs proved to be specific for the indicated target antigen. We conclude that the tested ABs are unsuitable for immunohistochemistry in paraffin-embedded murine tissues.
authors.affiliation: Department of Ophthalmology, University Medical Center, Johannes Gutenberg University Mainz, Langenbeckstr. 1, 55131, Mainz, Germany, to.boehmer@t-online.de.
authors.forename: Tobias
authors.initials: T
authors.lastname: Böhmer
authors.forename: Norbert
authors.initials: N
authors.lastname: Pfeiffer
authors.forename: Adrian
authors.initials: A
authors.lastname: Gericke
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D003331:Coronary Vessels / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D008638:Mesenteric Arteries / Q000378:metabolism; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016612:Paraffin Embedding; D021122:Protein Subunits / Q000276:immunology; D018340:Receptors, Adrenergic, alpha-1 / Q000235:genetics / Q000276:immunology*
chemical_list: C547714:Adra1a protein, mouse; C547717:Adra1b protein, mouse; D000906:Antibodies; D021122:Protein Subunits; D018340:Receptors, Adrenergic, alpha-1