title: [Influence of anticoagulant on the plasma level of fifteen biochemical parameters].
abstract: The study of the influence of the anticoagulant used in blood collection tubes to obtain plasma was performed for fifteen biochemical parameters measured with automated Cobas 6000 (Roche Diagnostics). For each parameter tested the entire measurement domain was studied. The comparison of results obtained on plasma blood sample obtained by lithium heparin and EDTA include: correlation, the limits of acceptability in the standards of monitoring and interpretation standards regression defined by the SFBC and analysis of Bland-Altman. The parameters studied were classified into three categories. The parameters for which the assay is not influenced by the nature of the anticoagulant used: apolipoprotéin A1, apolipoprotein B, alanine amino-transferase, creatine kinase, creatinine, total cholesterol, HDL-cholesterol, lipase, NT-Pro BNP, troponine T and urea. The parameters for which the results are underestimated EDTA plasma, including those for which the impact is moderate and for which the interpretive standards are not changed: triglycerides, and those for which performance standards are changed on one or more levels: aspartate aminotransferase and lactate dehydrogenase; and finally the not practicable EDTA plasma parameters: alkaline phosphatase.
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Sacchetto
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Ali
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Erwan
authors.initials: E
authors.lastname: Dumontet
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Le Carrer
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Orsonneau
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Delaroche
authors.affiliation: Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
authors.forename: Edith
authors.initials: E
authors.lastname: Bigot-Corbel
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000925:Anticoagulants / Q000494:pharmacology*; D016632:Apolipoprotein A-I / Q000097:blood; D001055:Apolipoproteins B / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001322:Autoanalysis / Q000295:instrumentation; D001774:Blood Chemical Analysis; D001800:Blood Specimen Collection / Q000295:instrumentation*; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D003404:Creatinine / Q000097:blood; D004492:Edetic Acid / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008049:Lipase / Q000097:blood; D018020:Lithium Compounds / Q000494:pharmacology; D020097:Natriuretic Peptide, Brain / Q000097:blood; D010446:Peptide Fragments / Q000097:blood; D014280:Triglycerides / Q000097:blood; D020107:Troponin T / Q000097:blood; D014508:Urea / Q000097:blood
chemical_list: D000925:Anticoagulants; D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D018020:Lithium Compounds; D010446:Peptide Fragments; D014280:Triglycerides; D020107:Troponin T; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain; D014508:Urea; D006493:Heparin; D002784:Cholesterol; D004492:Edetic Acid; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase; D008049:Lipase; D000469:Alkaline Phosphatase
keywords: EDTA; comparison; lithium heparin; monitoring standards; performance standards