title: Impact of organic loading rate on biohydrogen production in an up-flow anaerobic packed bed reactor (UAnPBR).
abstract: This study assesses the impact of organic loading rate on biohydrogen production from glucose in an up-flow anaerobic packed bed reactor (UAnPBR). Two mesophilic UAPBRs (UAnPBR1 and 2) were tested at organic loading rates (OLRs) ranging from 6.5 to 51.4 g COD L(-1)d(-1). To overcome biomass washout, design modifications were made in the UAnPBR2 to include a settling zone to capture the detached biomass. The design modifications in UAnPBR2 increased the average hydrogen yield from 0.98 to 2.0 mol-H2 mol(-1)-glucose at an OLR of 25.7 g COD L(-1)d(-1). Although, a maximum hydrogen production rate of 23.4 ± 0.9 L H2 L(-1)d(-1) was achieved in the UAnPBR2 at an OLR of 51.4 g COD L(-1)d(-1), the hydrogen yield dropped by 50% to around 1 mol-H2 mol(-1)-glucose. The microbiological analysis (PCR/DGGE) showed that the biohydrogen production was due to the presence of the hydrogen and volatile acid producers such as Clostridium beijerinckii, Clostridium butyricum, Megasphaera elsdenii and Propionispira arboris.
authors.affiliation: Biological Processes Laboratory, Center for Research, Development and Innovation in Environmental Engineering, São Carlos School of Engineering (EESC), University of São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, 13563-120 São Carlos, SP, Brazil; Dept. of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario N6A 5B9, Canada.
authors.forename: Antônio Djalma Nunes
authors.initials: AD
authors.lastname: Ferraz
authors.affiliation: Biological Processes Laboratory, Center for Research, Development and Innovation in Environmental Engineering, São Carlos School of Engineering (EESC), University of São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, 13563-120 São Carlos, SP, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Zaiat
authors.affiliation: Dept. of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario N6A 5B9, Canada.
authors.forename: Medhavi
authors.initials: M
authors.lastname: Gupta
authors.affiliation: Dept. Civil and Environmental Engineering, University of Waterloo, London, Ontario N2L 3G1, Canada. Electronic address: sayedbesh@yahoo.com.
authors.forename: Elsayed
authors.initials: E
authors.lastname: Elbeshbishy
authors.affiliation: Greenfield Ethanol, 540 Park Avenue East, Chatham, Ontario N7M 5J4, Canada.
authors.forename: Hisham
authors.initials: H
authors.lastname: Hafez
authors.affiliation: Dept. of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario N6A 5B9, Canada.
authors.forename: George
authors.initials: G
authors.lastname: Nakhla
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects; D001419:Bacteria / Q000235:genetics; D056804:Biofuels; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D058645:Denaturing Gradient Gel Electrophoresis; D005285:Fermentation / Q000187:drug effects; D006859:Hydrogen / Q000378:metabolism*; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000494:pharmacology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012212:Rheology; D012995:Solubility; D013997:Time Factors; D014835:Volatilization / Q000187:drug effects
chemical_list: D056804:Biofuels; D009930:Organic Chemicals; D012336:RNA, Ribosomal, 16S; D006859:Hydrogen
keywords: Attached biomass; Biohydrogen; Organic loading rate; Packed bed reactor; Settling zone