title: Bacillus cheonanensis sp. nov. isolated from near poultry farm soil [corrected].
abstract: A novel bacterial strain, designated PFS-5(T), was isolated from the soil environment with feces of a live poultry farm located in Cheonan, Republic of Korea. Strain PFS-5(T) was Gram-staining-positive, motile, strictly aerobic bacterium, rod-shaped, and endospore-forming. The strain contained meso-diaminopimelic acid in their peptidoglycan and MK-7 menaquinone. The major fatty acids were anteiso-C15:0 (44.2%), C16:0 (22.2%), and iso-C15:0 (16.7%). The DNA G+C content was 40.1 mol%. Comparative 16S rRNA gene sequence analysis identified strain PFS-5(T) in the genus Bacillus, exhibiting the highest level of sequence similarity with type strain of B. herbersteinensis D-1,5a(T) (96.9%), B. humi LMG 22167(T) (96.7%), B. alkalitelluris BA288(T) (96.1%), B. litoralis SW-211(T) (96.0%), and B. luteolus YIM93174(T) (95.5%). The major polar lipids of PFS-5(T) were diphosphatidylglycerol and phosphatidylglycerol. On the basis of result from poly-phasic data, strain PFS-5(T) represents a novel species, for which the name Bacillus cheonanensis sp. nov. is proposed (Type strai PFS-5(T) = KACC 17469(T) = JCM19333(T)).
authors.affiliation: Department of Microbiology, College of Natural Sciences, Dankook University, Cheonan, 330-714, Republic of Korea.
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Kim
authors.forename: Cheol-Su
authors.initials: CS
authors.lastname: Park
authors.forename: Siwon
authors.initials: S
authors.lastname: Lee
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Ahn
mesh_terms: D000332:Aerobiosis; D000818:Animals; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008124:Locomotion; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011200:Poultry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid