title: Macromolecular sensing of RNAs by exploiting conformational changes in supramolecular nanostructures.
abstract: Here, we report on a ratiometric fluorescence biosensor based on self-assembled peptide nanostructures (SPN), which can respond to conformational changes induced by RNA ligand binding. The design of the SPN biosensor was inspired by the conformational stabilization and multimerization behaviors of the HIV-1 Rev protein induced by cooperative protein-protein and protein-RNA interactions. Because conformation-sensitive SPN biosensors can orchestrate binding and signal transduction events, they can be developed as highly sophisticated and smart nanomaterials for biosensing.
authors.affiliation: Translational Research Center for Protein Function Control and Department of Materials Science and Engineering, Yonsei University , Seoul 120-749, Korea.
authors.forename: So-hee
authors.initials: SH
authors.lastname: Han
authors.forename: Hee-won
authors.initials: HW
authors.lastname: Kim
authors.forename: Woo-jin
authors.initials: WJ
authors.lastname: Jeong
authors.forename: Yong-beom
authors.initials: YB
authors.lastname: Lim
mesh_terms: D015374:Biosensing Techniques; D005456:Fluorescent Dyes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D055503:Protein Multimerization; D055550:Protein Stability; D012331:RNA, Fungal / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014961:X-Ray Diffraction; D054321:rev Gene Products, Human Immunodeficiency Virus / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D012331:RNA, Fungal; D054321:rev Gene Products, Human Immunodeficiency Virus; C514860:rev protein, Human Immunodeficiency Virus-1