title: Flow cytometry-based analysis of artemisinin-resistant Plasmodium falciparum in the ring-stage survival assay.
abstract: The ring-stage survival assay (RSA) is a powerful tool for phenotyping artemisinin-resistant Plasmodium falciparum but requires experienced microscopists to count viable parasites among 10,000 erythrocytes in Giemsa-stained thin blood smears. Here we describe a rapid flow cytometric assay that accurately counts viable parasites among 250,000 erythrocytes in suspension. This method performs as well as light microscopy and can be used to standardize the collection of RSA data between research groups in laboratory and field settings.
authors.affiliation: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Chanaki
authors.initials: C
authors.lastname: Amaratunga
authors.affiliation: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Aaron T
authors.initials: AT
authors.lastname: Neal
authors.affiliation: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA rfairhurst@niaid.nih.gov.
authors.forename: Rick M
authors.initials: RM
authors.lastname: Fairhurst
mesh_terms: D000962:Antimalarials / Q000494:pharmacology*; D037621:Artemisinins / Q000494:pharmacology*; D001681:Biological Assay; D002470:Cell Survival; D004912:Erythrocytes / Q000469:parasitology / Q000648:ultrastructure; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006207:Half-Life; D006801:Humans; D008018:Life Cycle Stages / Q000187:drug effects*; D021261:Parasitic Sensitivity Tests; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000962:Antimalarials; D037621:Artemisinins; D005456:Fluorescent Dyes; C039060:artenimol; C031327:artemisinin