title: miRNA modulation of SOCS1 using an influenza A virus delivery system.
abstract: Difficulties associated with efficient delivery and targeting of miRNAs to cells is hampering the real world application of miRNA technology. This study utilized an influenza A-based delivery system to express miR-155 in order to knockdown SOCS1 mRNA. Using qPCR and dual luciferase technology we show that miR-155 delivery resulted in a significant increase in cellular miR-155 which facilitated a downregulation of SOCS1 gene expression and a functional increase in IL-6 and IFN-β cytokines.
authors.affiliation: School of Medicine, Deakin University, Geelong, Victoria, Australia.
authors.forename: Leonard
authors.initials: L
authors.lastname: Izzard
authors.affiliation: School of Medicine, Deakin University, Geelong, Victoria, Australia.
authors.forename: Siying
authors.initials: S
authors.lastname: Ye
authors.affiliation: CSIRO, Australian Animal Health Laboratory, Geelong, Victoria, Australia.
authors.forename: Kristie
authors.initials: K
authors.lastname: Jenkins
authors.affiliation: School of Medicine, Deakin University, Geelong, Victoria, Australia.
authors.forename: Yingju
authors.initials: Y
authors.lastname: Xia
authors.affiliation: CSIRO, Australian Animal Health Laboratory, Geelong, Victoria, Australia.
authors.forename: Mark
authors.initials: M
authors.lastname: Tizard
authors.affiliation: School of Medicine, Deakin University, Geelong, Victoria, Australia.
authors.forename: John
authors.initials: J
authors.lastname: Stambas
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004285:Dogs; D055785:Gene Knockdown Techniques; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D009980:Influenza A virus / Q000235:genetics*; D016899:Interferon-beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis / Q000235:genetics*; D014709:Vero Cells
chemical_list: D015850:Interleukin-6; D035683:MicroRNAs; C532623:Mirn155 microRNA, mouse; C491590:Socs1 protein, mouse; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D016899:Interferon-beta