title: O-GlcNAcylation is increased in prostate cancer tissues and enhances malignancy of prostate cancer cells.
abstract: O-GlcNAc is an O-linked ?-N-acetylglucosamine moiety attached to the side-chain hydroxyl of a serine or threonine residue in numerous cytoplasmic and nuclear proteins. In this study, we detected the level of O-GlcNAc in prostate, liver and pancreatic cancer tissues, and found that the global O-GlcNAc modification also known as O-GlcNAcylation, is specifically increased in prostate cancer tissues compared to corresponding adjacent tissues. In addition, we found that global O-GlcNAcylation is increased in prostate cancer cells and not in benign prostatic hyperplasia (BPH) epithelial cells. O-GlcNAc enhanced the anchorage-independent growth and the migratory/invasive ability of prostate cancer cells. More importantly, we provide here, for the first time to the best of our knowledge, direct evidence that increased O-GlcNAcylation induces malignant transformation of nontumorigenic (BPH) cells. Furthermore, our study suggested that inhibiting the formation of the E-cadherin/catenin/cytoskeleton complex may underly the O-GlcNAc-induced prostate cancer progression. Overall, these findings indicated that O-GlcNAcylation is increased in prostate, but not in liver and pancreatic cancer tissues, and that O-GlcNAc can enhance the malignancy of prostate cancer cells.
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, Key Laboratory of Glycoscience and Glycotechnology of Shandong Province, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, P.R. China.
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Urology Surgery, Qingdao Municipal Hospital, Qingdao 266011, P.R. China.
authors.forename: Jiangang
authors.initials: J
authors.lastname: Gao
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, Key Laboratory of Glycoscience and Glycotechnology of Shandong Province, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, P.R. China.
authors.forename: Cuifang
authors.initials: C
authors.lastname: Han
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, Key Laboratory of Glycoscience and Glycotechnology of Shandong Province, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, P.R. China.
authors.forename: Xinling
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, Key Laboratory of Glycoscience and Glycotechnology of Shandong Province, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, P.R. China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, Key Laboratory of Glycoscience and Glycotechnology of Shandong Province, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, P.R. China.
authors.forename: Leina
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Urology Surgery, Qingdao Municipal Hospital, Qingdao 266011, P.R. China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, Key Laboratory of Glycoscience and Glycotechnology of Shandong Province, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, P.R. China.
authors.forename: Wengong
authors.initials: W
authors.lastname: Yu
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism; D051177:Catenins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D008297:Male; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D015820:Cadherins; D051177:Catenins; D017351:N-Acetylglucosaminyltransferases; C449880:O-GlcNAc transferase; D000117:Acetylglucosamine