title: Alpha-mangostin suppresses IL-6 and IL-8 expression in P. gingivalis LPS-stimulated human gingival fibroblasts.
abstract: This study aims to investigate the effect of alpha-mangostin on interleukin (IL)-6 and IL-8 expression in human gingival fibroblasts (HGFs). HGFs were challenged with Porphyromonas gingivalis LPS and then treated with various concentrations of alpha-mangostin. The cytotoxicity was determined using MTS assay and cytokine expressions were evaluated by Real-time PCR and ELISA. The results showed that 5 μg/ml P. gingivalis LPS and alpha-mangostin at 1 µg/ml or less did not affect the viability of HGFs. Alpha-mangostin reduced IL-6 and IL-8 mRNA and protein in P. gingivalis LPS-stimulated HGFs. These findings suggested that alpha-mangostin might be used as an adjunct to the periodontal therapy.
authors.affiliation: Departments of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, 65000, Thailand. ichayak@yahoo.com.
authors.forename: Ichaya
authors.initials: I
authors.lastname: Yiemwattana
authors.affiliation: Department of Oral Diagnosis, Faculty of Dentistry, Naresuan University, Phitsanulok, 65000, Thailand.
authors.forename: Ruchadaporn
authors.initials: R
authors.lastname: Kaomongkolgit
mesh_terms: D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000276:immunology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D007113:Immunity, Innate; D015850:Interleukin-6 / Q000276:immunology*; D016209:Interleukin-8 / Q000276:immunology*; D008070:Lipopolysaccharides; D016966:Porphyromonas gingivalis / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D044004:Xanthones; C021053:mangostin
keywords: Alpha-mangostin; HGFs; IL-6/8