title: Carbon isotope ratios document that the elytra of western corn rootworm (Coleoptera: Chrysomelidae) reflects adult versus larval feeding and later instar larvae prefer Bt corn to alternate hosts.
abstract: In much of the Corn Belt and parts of Europe, the western corn rootworm, Diabrotica virgifera virgifera LeConte, is the most important insect pest of maize. The need for additional basic knowledge of this pest has been highlighted while developing resistance management plans for insecticidal genetically modified crops. This study evaluated the possibility of tracking feeding habits of western corn rootworm larvae using stable carbon isotope signatures. Plants accumulate different ratios of (13)C:(12)C isotopes, usually expressed as δ(13)C, according to whether they use the C3 or C4 photosynthetic pathway. Herbivore biomass is expected to reflect the δ(13)C of the food they eat. For the current experiment, western corn rootworm larvae were grown on different species of plants exhibiting different δ(13)C values. The δ(13)C values were then measured in elytra of emerged beetles. When beetles were unfed, biomass reflected larval feeding. When beetles were fed for 31 d postemergence, δ(13)C values of elytra almost exclusively reflected adult feeding. These results suggest the use of caution in the interpretation of δ(13)C data aiming to document larval diet history when adult feeding history is unknown. The technique was also used to evaluate western corn rootworm larval choice between alternate hosts and maize with and without genetically modified (Bt) traits aimed at their control. Propensity for feeding on alternate hosts versus maize was biased toward feeding on maize regardless whether the maize had Bt or not, suggesting western corn rootworm larvae were not repelled by Bt. These data will be helpful for regulators in interpreting western corn rootworm feeding data on Bt maize.
authors.affiliation: Division of Plant Science, University of Missouri, 205 Curtis Hall, Columbia, MO 65211, USA.
authors.forename: Ivan
authors.initials: I
authors.lastname: Hiltpold
authors.forename: John J
authors.initials: JJ
authors.lastname: Adamczyk
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Higdon
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Clark
authors.forename: Mark R
authors.initials: MR
authors.lastname: Ellersieck
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Hibbard
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000737:chemistry / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism; D001517:Coleoptera / Q000254:growth & development / Q000502:physiology*; D004731:Endotoxins / Q000235:genetics / Q000378:metabolism; D005247:Feeding Behavior; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000254:growth & development / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D006109:Poaceae / Q000737:chemistry; D013997:Time Factors; D014921:Wings, Animal / Q000737:chemistry / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D002244:Carbon