title: Current studies on sucrose isomerase and biological isomaltulose production using sucrose isomerase.
abstract: Isomaltulose is a natural isomer of sucrose. It is widely used as a functional sweetener with promising properties, including slower digestion, lower glycemic index, prolonged energy release, lower insulin reaction, and less cariogenicity. It has been approved as a safe sucrose substitute by the Food and Drug Administration of the US; Ministry of Health, Labor and Welfare of Japan; and the Commission of the European Communities. This article presents a review of recent studies on the properties, physiological effects, and food application of isomaltulose. In addition, the biochemical properties of sucrose isomerases producing isomaltulose are compared; the heterologous expression, fermentation optimization, structural determination, and catalysis mechanism of sucrose isomerase are reviewed; and the biotechnological production of isomaltulose from sucrose is summarized.
authors.affiliation: State Key Laboratory of Food Science and Technology, Ministry of Education Key Laboratory of Carbohydrate Chemistry and Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, China, wmmu@jiangnan.edu.cn.
authors.forename: Wanmeng
authors.initials: W
authors.lastname: Mu
authors.forename: Wenjing
authors.initials: W
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
mesh_terms: D000818:Animals; D055162:Biocatalysis; D001709:Biotechnology; D005964:Glucosyltransferases / Q000737:chemistry*; D006801:Humans; D007534:Isomaltose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D013549:Sweetening Agents / Q000737:chemistry* / Q000378:metabolism
chemical_list: D013549:Sweetening Agents; D007534:Isomaltose; D005964:Glucosyltransferases; C116853:sucrose isomerase; C008189:isomaltulose