title: Whole genome analyses of marine fish pathogenic isolate, Mycobacterium sp. 012931.
abstract: Mycobacterium is a genus within the order Actinomycetales that comprises of a large number of well-characterized species, several of which includes pathogens known to cause serious disease in human and animal. Here, we report the whole genome sequence of Mycobacterium sp. strain 012931 isolated from the marine fish, yellowtail (Seriola quinqueradiata). Mycobacterium sp. 012931 is a fish pathogen causing serious damage to aquaculture farms in Japan. DNA dot plot analysis showed that Mycobacterium sp. 012931 was more closely related to Mycobacterium marinum when compared across several Mycobacterium species. However, little conservation of the gene order was observed between Mycobacterium sp. 012931 and M. marinum genome. The annotated 5,464 genes of Mycobacterium sp. 012931 was classified into 26 subsystems. The insertion/deletion gene analysis shows Mycobacterium sp. 012931 had 643 unique genes that were not found in the M. marinum strains. In the virulence, disease, and defense subsystem, both insertion and deletion genes of Mycobacterium sp. 012931 were associated with the PPE gene cluster of Mycobacteria. Of seven plcB genes in Mycobacterium sp. 012931, plcB_2 and plcB_3 showed low identities with those of M. marinum strains. Therefore, Mycobacterium sp. 012931 has differences on genetic and virulence from M. marinum and may induce different interaction mechanisms between host and pathogen.
authors.affiliation: Agricultural and Veterinary Division, Animal Health Department of Research and Development, Meiji Seika Pharma, 2-4-16, Kyobashi, Chuo-ku, Tokyo, 104-8002, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kurokawa
authors.forename: Jun
authors.initials: J
authors.lastname: Kabayama
authors.forename: Seong Don
authors.initials: SD
authors.lastname: Hwang
authors.forename: Seong Won
authors.initials: SW
authors.lastname: Nho
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Hikima
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Jung
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.forename: Haruko
authors.initials: H
authors.lastname: Takeyama
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Mori
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D023061:Gene Order / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D023281:Genomics / Q000379:methods; D007564:Japan; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics*; D010473:Perciformes / Q000382:microbiology*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity