title: A novel panel of microRNAs provides a sensitive and specific tool for the diagnosis of breast cancer.
abstract: Breast cancer has a high fatality rate. Early diagnosis reduces the rate of mortality; therefore, novel diagnostic methods are urgently required. The present study investigated the correlation of serum microRNA (miRNA/miR) expression with breast cancer, and tested a panel of miRNAs as promising potential biomarkers for breast cancer. Six miRNAs (miR‑374, miR‑666‑5p, miR‑451, miR‑148a, miR‑27a and miR‑30b) were selected for analysis and their differential expression levels were quantified using qPCR. The results demonstrated that four out of the six candidate miRNAs were significantly downregulated in breast cancer patients (miR‑451, P=0.000; miR‑148a, P=0.021; miR‑27a, P=0.013 and miR‑30b, P=0.001). A panel of miRNAs consisting of the four downregulated miRNAs was able to distinguish breast cancer from healthy controls, with an area under the receiver operating characteristic curve of 95.3%, a sensitivity of 94.7% and a specificity of 82.8%. Thus, this panel of miRNAs may be used as a sensitive and specific tool for the diagnosis of breast cancer.
authors.affiliation: Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Luo
authors.affiliation: Medical College of Nankai University, Tianjin 300071, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Gynecology and Obstetrics, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Medical College of Nankai University, Tianjin 300071, P.R. China.
authors.forename: Zhuhong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: Medical College of Nankai University, Tianjin 300071, P.R. China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Haidian Hospital, Beijing 100080, P.R. China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Tian
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs