title: Danshensu inhibits β-adrenergic receptors-mediated cardiac fibrosis by ROS/p38 MAPK axis.
abstract: Danshensu, the effective ingredient of the plant Salvia miltiorrhiza (Danshen), has been widely used for treatment of cardiovascular diseases. Cardiac fibrosis is an important process in pathological cardiac remodeling and leads to heart failure. We investigated the effect of Danshensu on β-adrenergic receptor (β-AR)-mediated cardiac fibrosis and the involved signaling transduction. Danshensu inhibited cardiofibroblast proliferation and collagen I synthesis induced by isoproterenol (ISO), a selective β-AR agonist. Phosphorylation of p38 mitogen-activated protein kinase (MAPK), which mediates ISO-induced cardiac fibrosis, was negatively regulated in this process. The negative regulation depended on the ISO inhibition of reactive oxygen species (ROS) production. Taken together, Danshensu may inhibit β-AR-mediated cardiac fibrosis by negative regulation of ROS-p38 MAPK signaling.
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Lu
authors.forename: Aiju
authors.initials: A
authors.lastname: Tian
authors.forename: Jimin
authors.initials: J
authors.lastname: Wu
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.forename: Pu
authors.initials: P
authors.lastname: Jia
authors.forename: Lingjian
authors.initials: L
authors.lastname: Yang
authors.forename: Youyi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zheng
authors.forename: Zijian
authors.initials: Z
authors.lastname: Li
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D009202:Cardiomyopathies / Q000201:enzymology / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D005355:Fibrosis; D007545:Isoproterenol / Q000494:pharmacology; D007773:Lactates / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
chemical_list: D000318:Adrenergic beta-Agonists; D024042:Collagen Type I; D007773:Lactates; D017382:Reactive Oxygen Species; D011943:Receptors, Adrenergic, beta; C035055:3,4-dihydroxyphenyllactic acid; D007545:Isoproterenol