title: Qualitative analysis of the deposit of collagen in bladder suture of rats treated with tacrolimus combined with mycophenolate-mofetil.
abstract: OBJECTIVE
  To evaluate the synthesis of type I (mature) and type III (immature) collagen in bladder suture of rats treated with a combination of tacrolimus and mycophenolate mofetil for 15 days.
  
  
  METHODS
  Thirty rats were divided into 3 groups: the sham, control and experimental groups. All the animals underwent laparotomy, cystotomy and bladder suture in two planes with surgical PDS 5-0 thread. The sham group did not receive treatment. The control group received saline solution, and the experimental group received 0.1mg/kg/day of tacrolimus with 20mg/kg/day of mycophenolate mofetil, for 15 days. From then on, the tacrolimus was dosed. The surgical specimens of the bladder suture area were processed so that the total type I and type III collagen could be measured by the picrosirius red technique.
  
  
  RESULTS
  There was a predominance of type I collagen production in the sham and control groups compared to the experimental group, in which type III collagen was predominant. The production of total collagen did not change.
  
  
  CONCLUSIONS
  The association of tacrolimus and mycophenolate mofetil in animals qualitatively changes the production of collagen after 15 days with a predominance of type III collagen.
authors.affiliation: Department of Clinical Surgery. Health Sciences Sector of the Federal University of Parana (UFPR), Curitiba, PR, Brazil.
authors.forename: Gustavo Marquesine
authors.initials: GM
authors.lastname: Paul
authors.affiliation: Department of Clinical Surgery. Health Sciences Sector of the Federal University of Parana (UFPR), Curitiba, PR, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Tambara Filho
authors.affiliation: Department of Clinical Surgery. Health Sciences Sector of the Federal University of Parana (UFPR), Curitiba, PR, Brazil.
authors.forename: João Carlos Domingues
authors.initials: JC
authors.lastname: Repka
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000096:biosynthesis* / Q000187:drug effects; D024061:Collagen Type III / Q000096:biosynthesis* / Q000187:drug effects; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D009173:Mycophenolic Acid / Q000031:analogs & derivatives* / Q000627:therapeutic use; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013536:Suture Techniques; D013537:Sutures; D016559:Tacrolimus / Q000627:therapeutic use*; D016896:Treatment Outcome; D001743:Urinary Bladder / Q000601:surgery*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D007166:Immunosuppressive Agents; D009173:Mycophenolic Acid; D016559:Tacrolimus