title: Phenylene-diimine-capped conjugate of lower rim 1,3-calix[4]arene as molecular receptor for Mg2+ via arm conformational changes followed by aggregation and mimicking the species by molecular mechanics.
abstract: A phenylene-diimine-capped conjugate of lower rim 1,3-calix[4]arene (L) was synthesized, characterized, and shown to selectively bind to Mg(2+) using its capped arms. This results in a selective recognition of Mg(2+) through eliciting fluorescence enhancement of ∼70 fold with a detection limit of 40 ± 5 ppb. However, in the presence of blood serum, the lowest detection limit is 209 ± 10 ppb (0.2 μM). The binding of Mg(2+) to L is authenticated by absorption and (1)H NMR data. The Job's plot derived on the basis of the absorption data showed 1:1 stoichiometry between the receptor and Mg(2+). The 1:1 species was further confirmed through ESI MS, that is, being supported by the isotope peak pattern authenticating the presence of Mg(2+) in the complex. The L binds Mg(2+) octahedrally using the tetradentate L(2-) and two additional acetic acid moieties by bringing conformational changes as studied on the basis of MM computations. The conformational changes that occur in the capped arms upon Mg(2+) binding were supported experimentally by NOESY. AFM and SEM studies showed that spherical particles of L are modified into flower and chain type aggregates upon complexation with Mg(2+), confirming the supramolecular behavior of the species formed.
authors.affiliation: Department of Chemistry, Indian Institute of Technology , Powai, Mumbai - 400076, India.
authors.forename: Anita
authors.initials: A
authors.lastname: Nehra
authors.forename: Vijaya Kumar
authors.initials: VK
authors.lastname: Hinge
authors.forename: Chebrolu Pulla
authors.initials: CP
authors.lastname: Rao
mesh_terms: D047250:Calixarenes / Q000138:chemical synthesis / Q000737:chemistry*; D007097:Imines / Q000138:chemical synthesis* / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010636:Phenols / Q000138:chemical synthesis* / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D007097:Imines; D010636:Phenols; C121325:calix(4)arene; D047250:Calixarenes; C011981:phenyldiazene; D008274:Magnesium