title: Plasmodium knowlesi: clinical presentation and laboratory diagnosis of the first human case in a Scottish traveler.
abstract: The first imported case of Plasmodium knowlesi in Scotland is described in a 33-year-old female with a travel history to Borneo. The patient ceased to take antimalarial prophylaxis after 4 days of her 10-day visit and presented with a history of fever, rigor, vomiting, and diarrhea after 13 days on her return to the UK. Malaria antigen detection using the Optimal-IT and Binax-NOW kits was negative. Unusual trophozoite-like structures were observed under microscopic examination and the identification of P. knowlesi performed by a nested polymerase chain reaction (PCR) gel-based approach was confirmed by using a PCR-sequencing assay.
authors.affiliation: Department of Microbiology, Glasgow Royal Infirmary, NHS Greater Glasgow and Clyde, Glasgow, UK.
authors.forename: Claire J
authors.initials: CJ
authors.lastname: Cordina
authors.forename: Richard
authors.initials: R
authors.lastname: Culleton
authors.forename: Brian L
authors.initials: BL
authors.lastname: Jones
authors.forename: Catherine C
authors.initials: CC
authors.lastname: Smith
authors.forename: Alisdair A
authors.initials: AA
authors.lastname: MacConnachie
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Coyne
authors.forename: Claire L
authors.initials: CL
authors.lastname: Alexander
mesh_terms: D000328:Adult; D001893:Borneo; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008288:Malaria / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy; D016790:Plasmodium knowlesi / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012606:Scotland; D014195:Travel