title: CXC chemokine CXCL12 and its receptor CXCR4 in tree shrews (Tupaia belangeri): structure, expression and function.
abstract: Chemokines are small secreted proteins functionally involved in the immune system's regulation of lymphocyte migration across numerous mammalian species. Given its growing popularity in immunological models, we investigated the structure and function of chemokine CXCL12 protein in tree shrews. We found that CXCL12 and its receptor CXCR4 in tree shrew had structural similarities to their homologous human proteins. Phylogenetic analysis supports the view that tree shrew is evolutionarily-close to the primates. Our results also showed that the human recombinant CXCL12 protein directly enhanced the migration of tree shrew's lymphocytes in vitro, while AMD3100 enhanced the mobilization of hematopoietic progenitor cells (HPCs) from bone marrow into peripheral blood in tree shrew in vivo. Collectively, these findings suggested that chemokines in tree shrews may play the same or similar roles as those in humans, and that the tree shrew is a viable animal model for studying human immunological diseases.
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China; Department of Biochemistry & Molecular Biology, School of Basic Medicine, Dali University, Dali, China.
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China; Department of Rheumatology & Immunology, First Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China.
authors.forename: Shengke
authors.initials: S
authors.lastname: Meng
authors.affiliation: Department of Hepatobiliary Surgery, Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China.
authors.forename: Wenxue
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China.
authors.forename: Zongmin
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Cui
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China.
authors.forename: Meizhang
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology; D001596:Benzylamines; D054377:Chemokine CXCL12 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D000080027:Cyclams; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006571:Heterocyclic Compounds / Q000494:pharmacology; D006801:Humans; D018448:Models, Immunological; D010802:Phylogeny; D019718:Receptors, CXCR4 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014417:Tupaia / Q000235:genetics / Q000276:immunology
chemical_list: D019380:Anti-HIV Agents; D001596:Benzylamines; D054377:Chemokine CXCL12; D000080027:Cyclams; D006571:Heterocyclic Compounds; D019718:Receptors, CXCR4; C088327:plerixafor