title: Non-lysosomal degradation pathway for N-linked glycans and dolichol-linked oligosaccharides.
abstract: There is growing evidence that asparagine (N)-linked glycans play pivotal roles in protein folding and intra- or intercellular trafficking of N-glycosylated proteins. During the N-glycosylation of proteins, significant amounts of free oligosaccharides (fOSs) and phosphorylated oligosaccharides (POSs) are generated at the endoplasmic reticulum (ER) membrane by unclarified mechanisms. fOSs are also formed in the cytosol by the enzymatic deglycosylation of misfolded glycoproteins destined for proteasomal degradation. This article summarizes the current knowledge of the molecular and regulatory mechanisms underlying the formation of fOSs and POSs in mammalian cells and Saccharomyces cerevisiae.
authors.affiliation: Glycometabolome Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, RIKEN Global Research Cluster, Japan. Electronic address: tsuzuki_gm@riken.jp.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Glycometabolome Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, RIKEN Global Research Cluster, Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Harada
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D003600:Cytosol / Q000378:metabolism; D004286:Dolichols / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D008954:Models, Biological; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D017510:Protein Folding; D011755:Pyrophosphatases / Q000378:metabolism
chemical_list: D004286:Dolichols; D009844:Oligosaccharides; D011134:Polysaccharides; D011755:Pyrophosphatases
keywords: Free oligosaccharides; Non-lysosomal degradation; Oligosaccharyltransferase; Peptide:N-glycanase; Phosphorylated oligosaccharides; Pyrophosphatase