title: Immobilization of (R)- and (S)-amine transaminases on chitosan support and their application for amine synthesis using isopropylamine as donor.
abstract: Transaminases from Aspergillus fumigatus ((R)-selective, AspFum), Ruegeria pomeroyi ((S)-selective, 3HMU) and Rhodobacter sphaeroides 2.4.1 ((S)-selective, 3I5T) were immobilized on chitosan with specific activities of 99, 157, and 163U/g and acceptable yields (54, 21, and 23%, respectively) for glutaraldehyde (GA) immobilization. Besides GA, also divinylsulfone was used as linker molecule leading to a similar efficient immobilization for two enzymes, GibZea and NeoFis, whereas GA was superior in the other cases. Storage of the GA-immobilized enzymes for one month resulted in increased relative activities between 120 and 180%. The thermal stability was improved, especially for the GA-immobilized AspFum compared to the free enzyme after incubation for 4h at 60°C (10% vs. 235% residual activity). Especially after incubation of AspFum (free or immobilized) for 2h at 50°C a strongly increased activity was observed (up to 359% of the initial activity). This effect was studied in more detail, revealing that one heat activation prior and one after immobilization increased the overall immobilization efficiency. Recycling of the immobilized ATAs resulted only in a small reduction of activity after four batches. Asymmetric synthesis of (R)- or (S)-1-methyl-3-phenylpropylamine from the prostereogenic ketone using isopropylamine (IPA) as amino donor was applied with conversions up to 50% (AspFum) or 75% (3HMU). Except for NeoFis, all immobilized ATAs showed higher conversions compared to the free enzyme.
authors.affiliation: Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str 4, D-17487 Greifswald, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Mallin
authors.affiliation: Protein Biochemistry, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str 4, D-17487 Greifswald, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Höhne
authors.affiliation: Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str 4, D-17487 Greifswald, Germany. Electronic address: uwe.bornscheuer@uni-greifswald.de.
authors.forename: U T
authors.initials: UT
authors.lastname: Bornscheuer
mesh_terms: D000588:Amines / Q000138:chemical synthesis* / Q000737:chemistry; D055162:Biocatalysis; D048271:Chitosan / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D011437:Propylamines / Q000138:chemical synthesis / Q000737:chemistry*; D013696:Temperature; D000637:Transaminases / Q000737:chemistry*
chemical_list: D000588:Amines; D004800:Enzymes, Immobilized; D011437:Propylamines; D048271:Chitosan; D000637:Transaminases; C035263:2-propylamine
keywords: Asymmetric synthesis; Biocatalysis; Enzyme activation; Immobilization; Transaminase