title: Behaviors of MC3T3-E1 cells on carbonated apatite films, with a characteristic network structure, fabricated on a titanium plate by aqueous spray coating.
abstract: Four carbonated apatite films having average thicknesses of 1.3-0.11μm, proportions of network sizes above 10μm of 41-68%, and average border heights of the characteristic network structure of 0.98-0.29μm were fabricated on a titanium plate by aqueous spray coating. These carbonated apatite films after heat treatment showed good mineralization ability in Hanks' balanced salt solution. Assessment of initial cell attachment and calcination on these films and on the Ti plate using osteoblastic MC3T3-E1 indicated that the carbonated apatite film heat treated at 600°C, whose film thickness, proportion of network sizes above 10μm, and border height were 0.11μm, 61%, and 0.31μm, respectively, was most preferred by osteoblastic cells. Field emission scanning electron microscopic observation of the cells attached to the films showed that the wide network and low border height of the network structure on the carbonated apatite film play an important role in the development of the filopodia of the osteoblastic cells.
authors.affiliation: Division of Liberal Arts, Kogakuin University, 2665-1 Nakano, Hachioji, Tokyo 192-0015, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Mochizuki
authors.affiliation: Division of Liberal Arts, Kogakuin University, 2665-1 Nakano, Hachioji, Tokyo 192-0015, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hara
authors.affiliation: Research Institute for Science and Technology, Kogakuin University, 2665-1 Nakano, Hachioji, Tokyo 192-0015, Japan; School of Engineering, Tokai University, 4-1-1 Kitakanane, Hiratsuka, Kanagawa 259-1292, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Oya
authors.affiliation: Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo 191-0065, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Aoki
authors.affiliation: Department of Dental Engineering, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Yokohama City, Kanagawa 230-8501, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Hayakawa
authors.affiliation: Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo 191-0065, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Fujie
authors.affiliation: Division of Liberal Arts, Kogakuin University, 2665-1 Nakano, Hachioji, Tokyo 192-0015, Japan. Electronic address: lccsato@cc.kogakuin.ac.jp.
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Sato
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001031:Apatites / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001031:Apatites; C030782:carboapatite; D014025:Titanium
keywords: Apatite structure; Cell adhesion; Hydroxyapatite coating; Osteoblastic cell; Surface modification