title: Ethanol production from non-detoxified whole slurry of sulfite-pretreated empty fruit bunches at a low cellulase loading.
abstract: Sulfite pretreatment to overcome the recalcitrance of lignocelluloses (SPORL) was applied to an empty fruit bunches (EFB) for ethanol production. SPORL facilitated delignification through lignin sulfonation and dissolution of xylan to result in a highly digestible substrate. The pretreated whole slurry was enzymatically saccharified at a solids loading of 18% using a relatively low cellulase loading of 15 FPU/g glucan and simultaneously fermented without detoxification using Saccharomyces cerevisiae of YRH400. An ethanol yield of 217 L/tonne EFB was achieved at titer of 32 g/L. Compared with literature studies, SPORL produced high ethanol yield and titer with much lower cellulase loading without detoxification.
authors.affiliation: Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing, China; USDA Forest Service, Forest Products Lab, Madison, WI 53719, USA.
authors.forename: Jinlan
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Dept. Civil Environ. Eng., Hong Kong Polytechnic University, Kowloon, Hong Kong; USDA Forest Service, Forest Products Lab, Madison, WI 53719, USA.
authors.forename: Shao-Yuan
authors.initials: SY
authors.lastname: Leu
authors.affiliation: USDA Forest Service, Forest Products Lab, Madison, WI 53719, USA; Dept. Biological Systems Eng., University of Wisconsin-Madison, Madison, WI 53705, USA. Electronic address: jzhu@fs.fed.us.
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhu
authors.affiliation: Xylome Corporation, Madison, WI 53705, USA.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Jeffries
mesh_terms: D028023:Arecaceae / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005638:Fruit / Q000737:chemistry*; D005662:Furaldehyde / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D013379:Substrate Specificity / Q000187:drug effects; D013447:Sulfites / Q000494:pharmacology*; D013997:Time Factors; D014990:Xylans / Q000302:isolation & purification; D014994:Xylose / Q000096:biosynthesis
chemical_list: D011134:Polysaccharides; D013447:Sulfites; D014990:Xylans; D000431:Ethanol; C007916:hemicellulose; D014994:Xylose; D005662:Furaldehyde; D002480:Cellulase
keywords: Empty fruit bunches (EFB); Fermentation; High solids Enzymatic hydrolysis/saccharification; High titer ethanol; Pretreatment