title: Subcortical band heterotopia in rat offspring following maternal hypothyroxinaemia: structural and functional characteristics.
abstract: Thyroid hormones (TH) play crucial roles in brain maturation and are important for neuronal migration and neocortical lamination. Subcortical band heterotopia (SBH) represent a class of neuronal migration errors in humans that are often associated with childhood epilepsy. We have previously reported the presence of SBH in a rodent model of low level hypothyroidism induced by maternal exposure to the goitrogen, propylthiouracil (PTU). In the present study, we report the dose-response characteristics of this developmental malformation and the connectivity of heterotopic neurones with other brain regions, as well as their functionality. Pregnant rats were exposed to varying concentrations of PTU through the drinking water (0-10 p.p.m.) beginning on gestational day 6 to produce graded levels of TH insufficiency. Dose-dependent increases in the volume of the SBH present in the corpus callosum were documented in the adult offspring, with a clear presence at concentrations of PTU that resulted in minor (< 15%) reductions in maternal serum thyroxine as measured when pups were weaned. SBH contain neurones, oligodendrocytes, astrocytes and microglia. Monoaminergic and cholinergic processes were prevalent and many of the axons were myelinated. Anatomical connectivity of SBH neurones to cortical neurones and the synaptic functionality of these anatomical connections was verified by ex vivo field potential recordings. SBH persisted in adult offspring despite a return to euthyroid status on termination of exposure and these offspring displayed an increased sensitivity to seizures. Features of this model are attractive with respect to the investigation of the molecular mechanisms of cortical development, the effectiveness of therapeutic intervention in hypothyroxinaemia during pregnancy and the impact of the very modest TH imbalance that accompanies exposure to environmental contaminants.
authors.affiliation: Toxicity Assessment Division, Neurotoxicology Branch, US Environmental Protection Agency, Research Triangle Park, NC, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Gilbert
authors.forename: R L
authors.initials: RL
authors.lastname: Ramos
authors.forename: D P
authors.initials: DP
authors.lastname: McCloskey
authors.forename: J H
authors.initials: JH
authors.lastname: Goodman
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology / Q000503:physiopathology; D054221:Classical Lissencephalies and Subcortical Band Heterotopias / Q000150:complications / Q000473:pathology* / Q000503:physiopathology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007037:Hypothyroidism / Q000097:blood / Q000139:chemically induced / Q000150:complications*; D018811:Maternal Exposure / Q000009:adverse effects; D008564:Membrane Potentials; D056347:Neuroanatomical Tract-Tracing Techniques; D009457:Neuroglia / Q000473:pathology; D009474:Neurons / Q000473:pathology; D010433:Pentylenetetrazole / Q000494:pharmacology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology*; D011441:Propylthiouracil / Q000494:pharmacology; D051381:Rats; D012640:Seizures / Q000139:chemically induced / Q000150:complications; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood*; D014284:Triiodothyronine / Q000097:blood
chemical_list: D014284:Triiodothyronine; D011441:Propylthiouracil; D013972:Thyrotropin; D013974:Thyroxine; D010433:Pentylenetetrazole
keywords: foetal brain; hypothyroxinaemia; neuronal migration; thyroid hormone propylthiouracil