title: Pseudoscardovia radai sp. nov., another representative of a new genus within the family Bifidobacteriaceae isolated from the digestive tract of a wild pig ( Sus scrofa scrofa ).
abstract: Presence of bifidobacteria and representatives of the new genus Pseudoscardovia within the family Bifidobacteriaceae in the digestive tract of wild pigs has been reported recently. Results based on comparative 16S rRNA gene sequence analysis of a new fructose-6-phosphate phosphoketolase-positive bacterial isolate originated from the small intestine of a wild pig revealed a relationship to Pseudoscardovia suis DPTE4T (96.8% sequence similarity). Phylogenetic and comparative analyses based on 16S rRNA, hsp60, xfp, fusA, tuf and rpoC partial gene sequences confirmed relationship of the new bacterial strain to Pseudoscardovia suis compared with bifidobacteria species occurring in the digestive tract of domestic and wild pigs. Differences in utilization of various substrates, production of enzymes, cell morphology, peptidoglycan structure, profile of cellular fatty acids and polar lipids between the new bacterial isolate designated as DPVI-TET3T and P. suis DPTE4T allow to establish a new bacterial taxon for which the name Pseudoscardovia radai sp. nov. (= DPVI/TET3T = CCM 7943T = DSM 24742T) was proposed.
authors.affiliation: Institute of Animal Physiology and Genetics AS CR Prague; killer.jiri@seznam.cz.
authors.forename: Jiri
authors.initials: J
authors.lastname: Killer
authors.affiliation: Czech University of Life Sciences, Faculty of Agrobiology, Food and Natural Resources;
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Havlik
authors.affiliation: Czech University of Life Sciences, Faculty of Agrobiology, Food and Natural Resources;
authors.forename: Vera
authors.initials: V
authors.lastname: Bunesova
authors.affiliation: Czech University of Life Sciences, Faculty of Agrobiology, Food and Natural Resources;
authors.forename: Eva
authors.initials: E
authors.lastname: Vlkova
authors.affiliation: Institute of Microbiology v.v.i., Academy of Sciences of the Czech Republic.
authors.forename: Oldrich
authors.initials: O
authors.lastname: Benada
keywords: Bifidobacteriaceae; Multilocus sequence analysis; Pseudoscardovia; digestive tract; wild pigs