title: Paenibacillus dongdonensis sp. nov., isolated from rhizospheric soil of Elymus tsukushiensis.
abstract: A Gram-staining-positive, endospore-forming and rod-shaped bacterial strain, designated KUDC0114(T), was isolated from rhizospheric soil of Elymus tsukushiensis from Dongdo Island, one of the largest of the Dokdo Islands, South Korea. The strain displayed optimal growth at 37 °C, pH 8.5 in the absence of NaCl. Based on phylogenetic analysis of 16S rRNA gene sequences, strain KUDC0114(T) represented a member of the genus Paenibacillus and was most closely related to Paenibacillus taichungensis BCRC 17757(T) (98.46%). The cell-wall peptidoglycan was A1γ type, and the predominant quinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso-C(15 : 0) and C(16 : 0). The DNA-DNA hybridization of strain KUDC0114(T) with nine other strains indicated less than 23% relatedness, and its DNA G+C content was 44.30 mol%. Based on genomic, phenotypic and phylogenetic analyses, KUDC0114(T) should be classified as representing novel species within the genus Paenibacillus. The name Paenibacillus dongdonensis sp. nov. is proposed. The type strain is KUDC0114(T) ( = DSM27607(T) = KCTC33221(T)).
authors.affiliation: School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Son
authors.affiliation: School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Hyun-Uk
authors.initials: HU
authors.lastname: Kang
authors.affiliation: School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Sa-Youl
authors.initials: SY
authors.lastname: Ghim
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D031749:Elymus / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7