title: A protective role of methionine-R-sulfoxide reductase against cadmium in Schizosaccharomyces pombe.
abstract: The Schizosaccharomyces pombe cells harboring the methionine- R-sulfoxide reductase (MsrB)-overexpressing recombinant plasmid pFMetSO exhibited better growth than vector control cells, when shifted into fresh medium containing cadmium chloride (abbreviated as Cd). Although both groups of cells contained enhanced reactive oxygen species (ROS) and nitric oxide (NO) levels in the presence of Cd, ROS and NO levels were significantly lower in the S. pombe cells harboring pFMetSO than in vector control cells. Conversely, the S. pombe cells harboring pFMetSO possessed higher total glutathione (GSH) levels and a greater reduced/oxidized GSH ratio than vector control cells under the same conditions.
authors.affiliation: Department of Biochemistry, Kangwon National University, Chuncheon, 200-701, Republic of Korea.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Lim
authors.forename: Hannah
authors.initials: H
authors.lastname: Jo
authors.forename: Kyunghoon
authors.initials: K
authors.lastname: Kim
mesh_terms: D002104:Cadmium / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D051150:Methionine Sulfoxide Reductases / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000502:physiology*
chemical_list: D017382:Reactive Oxygen Species; D029702:Schizosaccharomyces pombe Proteins; D002104:Cadmium; D009569:Nitric Oxide; D051150:Methionine Sulfoxide Reductases; C000589817:MsrB protein, S pombe; D005978:Glutathione