title: Cryopreservation of adventitious shoot tips of Paraisometrum mileense by droplet vitrification.
abstract: BACKGROUND
  Gesneriaceae family contains numerous species endemic to China, and many of them are listed as endangered species. There is a need for a simple and efficient method for long-term conservation of these species.
  
  
  OBJECTIVE
  The study aimed to establish an efficient procedure for cryopreserving Paraisometrum mileense, a critically endangered species endemic to Yunnan, China.
  
  
  METHODS
  Effects of sucrose concentration of preculture solution, duration of sucrose preculture, duration of plant vitrification solution 2 (PVS2) treatment, and cold acclimation on regeneration of cryopreserved adventitious shoot tips (ASTs) were assessed.
  
  
  CONCLUSIONS
  Among different sucrose preculture regimes tested, preculture with 0.3M sucrose for 24h resulted in best regeneration of cryopreserved ASTs. PVS2 treatment also affected regeneration considerably with the maximum survival of ASTs after incubation in PVS2 for 90 min at 0 degrees C. With the optimised parameters, the level of shoot regeneration from cryopreserved ASTs reached 86%. No morphological abnormalities were observed during one year's growth of the plantlets developing from cryopreserved ASTs. Procedure established in this research is a promising technique for the cryopreservation of ASTs of this species.
authors.forename: Liang
authors.initials: L
authors.lastname: Lin
authors.forename: Bin
authors.initials: B
authors.lastname: Yuan
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.forename: Weiqi
authors.initials: W
authors.lastname: Lin
mesh_terms: D002681:China; D003247:Conservation of Natural Resources; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D056727:Endangered Species; D005079:Excipients / Q000494:pharmacology; D019686:Lamiaceae / Q000187:drug effects / Q000502:physiology*; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology*; D012038:Regeneration; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents; D005079:Excipients; D013395:Sucrose