title: An electrochemical aptasensor based on gold nanoparticles dotted graphene modified glassy carbon electrode for label-free detection of bisphenol A in milk samples.
abstract: A simple and label-free electrochemical aptasensor for bisphenol A (BPA) determination was developed based on gold nanoparticles dotted graphene (GNPs/GR) nanocomposite film modified glassy carbon electrode (GCE). The electrochemical probe of ferricyanide was used to investigate the interactions between aptamer and BPA. The resulting GNPs/GR layer exhibited good current response for BPA detection. The highly conductive and biocompatible nanostructure of GNPs/GR nanocomposite was characterised by atomic force microscope (AFM), scanning electron microscopy (SEM) and cyclic voltammetry (CV). The peak current change (ΔI) of ferricyanide was linear with the concentration of BPA in the range from 0.01 μM to 10 μM with the detection limit of 5 nM. The proposed aptasensor is rapid, convenient and low-cost for effective sensing of BPA. Particularly, the aptasensor was applied successfully to determine BPA in milk products, and the average recovery was 105%.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: jpwang@zju.edu.cn.
authors.forename: Jianping
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, Hangzhou 310058, China.
authors.forename: Dujuan
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000737:chemistry*; D002244:Carbon; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002244:Carbon; D006046:Gold; D006108:Graphite; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Aptamer; Bisphenol A; Electrochemical biosensor; Gold nanoparticles dotted graphene