title: Discrimination of leaves of Panax ginseng and P. quinquefolius by ultra high performance liquid chromatography quadrupole/time-of-flight mass spectrometry based metabolomics approach.
abstract: In present study, an ultra high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS) based metabolomics approach was established to investigate the metabolic profiles and characteristic chemical markers for distinguishing between leaves of Panax ginseng (LPG) and Panax quinquefolius (LPQ). The UHPLC-QTOF-MS/MS data were subjected to principal component analysis (PCA) and orthogonal partial least squared discrimination analysis (OPLS-DA) to rapidly find the potential characteristic components of LPG and LPQ, and the identities of detected peaks including the potential characteristic components were elucidated. Totally, 86 components were identified from these 2 kinds of leaf samples, in which 9 ginsenosides could be regarded as the characteristic chemical markers for the discrimination of LPG from LPQ. These results suggested that UHPLC-QTOF-MS/MS based metabolomics approach is a powerful tool to rapidly find characteristic markers for the quality control of LPG.
authors.affiliation: Department of Pharmaceutical Analysis & Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Department of Pharmaceutical Analysis & Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, PR China; State Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Bai
authors.affiliation: Department of Pharmaceutical Analysis & Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, PR China.
authors.forename: Jin-Di
authors.initials: JD
authors.lastname: Xu
authors.affiliation: Department of Pharmaceutical Analysis & Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Kong
authors.affiliation: Department of Pharmaceutical Analysis & Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, PR China.
authors.forename: Lin-Yin
authors.initials: LY
authors.lastname: Zhu
authors.affiliation: Department of Pharmaceutical Analysis & Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, PR China.
authors.forename: He
authors.initials: H
authors.lastname: Zhu
authors.affiliation: State Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing, PR China. Electronic address: qwang49@126.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Analysis & Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, PR China. Electronic address: songlinli64@126.com.
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D036145:Ginsenosides / Q000032:analysis*; D016018:Least-Squares Analysis; D055432:Metabolomics / Q000379:methods*; D005894:Panax / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D025341:Principal Component Analysis; D012503:Saponins / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D036145:Ginsenosides; C079995:Panax quinquefolium saponin; D012503:Saponins
keywords: Discrimination; Leaves of P. ginseng; Leaves of P. quinquefolius; Metabolomics; UHPLC–QTOF-MS/MS