title: Prognostic significance of TMEM16A, PPFIA1, and FADD expression in invasive ductal carcinoma of the breast.
abstract: BACKGROUND
  11q13 region is a frequently amplified locus in human malignancies. Among the genes located in this region, FADD is one of the alleged driving genes. Because amplification is not generally confined to a single gene and amplified genes may not show increased expression, we need to evaluate clinical significance of changes occurring in 11q13 region to understand their roles in carcinogenesis. Therefore, we screened expressions of FADD and closely located genes (PPFIA1 and TMEM16A) and evaluated the expressions to find clinical significance in invasive ductal carcinoma of the breast.
  
  
  METHODS
  Ninety-eight cases of invasive ductal carcinoma of the breast were collected. Using archival tissues resected from the cases, we built a tissue microarray and used it in immunohistochemistry. We evaluated the association of FADD, PPFIA1, and TMEM16A expression scores with clinicopathological parameters, including disease-free survival.
  
  
  RESULTS
  FADD expression was associated with T stage (P=0.046). The combined score of FADD, PPFIA1, and TMEM16A gene expressions was associated with perineural invasion (P=0.022). Although individual gene expressions of TMEM16A, FADD, and PPFIA1 failed to show significant association with disease-free survival, combined gene expression scores did show association with disease-free survival (P=0.034).
  
  
  CONCLUSIONS
  FADD, TMEM16A, and PPFIA1 gene expressions as a whole were associated with disease-free survival in breast cancer.
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Choi
authors.forename: Jeong A
authors.initials: JA
authors.lastname: Yun
authors.forename: Sahrish
authors.initials: S
authors.lastname: Jabeen
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Jeon
authors.forename: Hye Sung
authors.initials: HS
authors.lastname: Won
authors.forename: Yoon Ho
authors.initials: YH
authors.lastname: Ko
authors.affiliation: Department of Pathology, The Catholic University of Korea, School of Medicine, Seochogu Banpodaero 222, Seoul 137-701, South Korea. suyoung@catholic.ac.kr.
authors.forename: Su Young
authors.initials: SY
authors.lastname: Kim
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000075369:Anoctamin-1; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000473:pathology; D018118:Chloride Channels / Q000378:metabolism*; D053200:Fas-Associated Death Domain Protein / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D060787:Neoplasm Grading; D009363:Neoplasm Proteins / Q000378:metabolism*; D009364:Neoplasm Recurrence, Local / Q000378:metabolism* / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis
chemical_list: C476213:ANO1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D000075369:Anoctamin-1; D014408:Biomarkers, Tumor; D018118:Chloride Channels; C505533:FADD protein, human; D053200:Fas-Associated Death Domain Protein; D009363:Neoplasm Proteins; C094398:PPFIA1 protein, human