title: Except for C-C chemokine receptor 7 expression, monocyte-derived dendritic cells from patients with multiple sclerosis are functionally comparable to those of healthy controls.
abstract: OBJECTIVE
  Dendritic cell (DC)-based immunotherapy has shown potential to counteract autoimmunity in multiple sclerosis (MS).
  
  
  METHODS
  We compared the phenotype and T-cell stimulatory capacity of in vitro generated monocyte-derived DC from MS patients with those from healthy controls.
  
  
  RESULTS
  Except for an increase in the number of C-C chemokine receptor 7-expressing DC from MS patients, no major differences were found between groups in the expression of maturation-associated membrane markers or in the in vitro capacity to stimulate autologous T cells.
  
  
  CONCLUSIONS
  Our observations may pave the way for the development of patient-tailored DC-based vaccination strategies to treat MS.
authors.affiliation: Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (Vaxinfectio), Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, Belgium.
authors.forename: Amber H
authors.initials: AH
authors.lastname: Nuyts
authors.affiliation: Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (Vaxinfectio), Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Ponsaerts
authors.affiliation: Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (Vaxinfectio), Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, Belgium.
authors.forename: Viggo F I
authors.initials: VF
authors.lastname: Van Tendeloo
authors.affiliation: Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (Vaxinfectio), Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, Belgium.
authors.forename: Wai-Ping
authors.initials: WP
authors.lastname: Lee
authors.affiliation: Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (Vaxinfectio), Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, Belgium.
authors.forename: Barbara
authors.initials: B
authors.lastname: Stein
authors.affiliation: Department of Neurology, National Center for Multiple Sclerosis, Melsbroek, Belgium and Center for Neurosciences, Universitair Ziekenhuis Brussel en Vrije Universiteit Brussel, Belgium.
authors.forename: Guy
authors.initials: G
authors.lastname: Nagels
authors.affiliation: Department of Neurology, National Center for Multiple Sclerosis, Melsbroek, Belgium and Center for Neurosciences, Universitair Ziekenhuis Brussel en Vrije Universiteit Brussel, Belgium.
authors.forename: Marie B
authors.initials: MB
authors.lastname: D'hooghe
authors.affiliation: Laboratory of Neurology, Born Bunge Institute, Translational Neurosciences, Faculty and Health Sciences, University of Antwerp and Division of Neurology, Antwerp University Hospital, Edegem, Belgium.
authors.forename: Barbara
authors.initials: B
authors.lastname: Willekens
authors.affiliation: Laboratory of Neurology, Born Bunge Institute, Translational Neurosciences, Faculty and Health Sciences, University of Antwerp and Division of Neurology, Antwerp University Hospital, Edegem, Belgium.
authors.forename: Patrick
authors.initials: P
authors.lastname: Cras
authors.affiliation: Department of Scientific Coordination and Biostatistics, Antwerp University Hospital, Edegem, Belgium.
authors.forename: Kristien
authors.initials: K
authors.lastname: Wouters
authors.affiliation: Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (Vaxinfectio), Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, Belgium.
authors.forename: Herman
authors.initials: H
authors.lastname: Goossens
authors.affiliation: Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (Vaxinfectio), Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, Belgium.
authors.forename: Zwi N
authors.initials: ZN
authors.lastname: Berneman
authors.affiliation: Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (Vaxinfectio), Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, Belgium. Electronic address: nathalie.cools@uza.be.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Cools
mesh_terms: D000328:Adult; D000368:Aged; D002454:Cell Differentiation / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D006801:Humans; D007167:Immunotherapy; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D009103:Multiple Sclerosis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D054400:Receptors, CCR7 / Q000096:biosynthesis* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination
chemical_list: D054400:Receptors, CCR7
keywords: T cell activation; dendritic cells; immunotherapy; multiple sclerosis