title: In vivo molecular imaging of cathepsin and matrix metalloproteinase activity discriminates between arthritic and osteoarthritic processes in mice.
abstract: Rheumatoid arthritis (RA) and osteoarthritis (OA) are serologically and clinically distinctive, but at the local level, both diseases have many molecular pathways in common. In vivo molecular imaging can unravel the local pathologic processes involved in both diseases. In this study, we investigated matrix metalloproteinase (MMP) and cathepsin activity during cartilage destruction, in an RA and an OA mouse model, using biophotonic imaging of substrate-based probes. Mice with collagen-induced arthritis (CIA) or destabilization of the medial meniscus (DMM) were imaged using near-infrared fluorescent probes, activated by several cathepsins or MMPs. Fluorescence signal intensity was compared to synovial gene expression, histology, and cartilage staining of a neoepitope of aggrecan cleaved by MMPs with the amino acids DIPEN. Increased cathepsin and MMP activity was seen during CIA, whereas the DMM model only showed increased MMP activity. DIPEN expression was seen only during CIA. A possible explanation can be differences in gene expressions; MMP3 and -13, known to produce DIPEN neoepitopes, were upregulated in the CIA model, whereas MMP12, known to be involved in elastin degradation and chemokine inhibition, was upregulated in the DMM model. Thus, molecular imaging showed no cathepsin activity at the time of cartilage damage in the DMM model, whereas both cathepsins and MMPs are active in the CIA model during disease progression.
authors.forename: Eline A
authors.initials: EA
authors.lastname: Vermeij
authors.forename: Marije I
authors.initials: MI
authors.lastname: Koenders
authors.forename: Arjen B
authors.initials: AB
authors.lastname: Blom
authors.forename: Onno J
authors.initials: OJ
authors.lastname: Arntz
authors.forename: Miranda B
authors.initials: MB
authors.lastname: Bennink
authors.forename: Wim B
authors.initials: WB
authors.lastname: van den Berg
authors.forename: Peter L E M
authors.initials: PL
authors.lastname: van Lent
authors.forename: Fons A J
authors.initials: FA
authors.lastname: van de Loo
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D002403:Cathepsins / Q000032:analysis* / Q000378:metabolism; D016923:Cell Death; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D024043:Collagen Type II / Q000009:adverse effects / Q000276:immunology; D008297:Male; D020782:Matrix Metalloproteinases / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D057054:Molecular Imaging / Q000379:methods*; D010003:Osteoarthritis / Q000378:metabolism*; D013264:Stifle / Q000737:chemistry / Q000378:metabolism / Q000473:pathology
chemical_list: D024043:Collagen Type II; D002403:Cathepsins; D020782:Matrix Metalloproteinases