title: Sulfated glucan can improve the immune efficacy of Newcastle disease vaccine in chicken.
abstract: To evaluate the immune effect of sulfated glucan from saccharomyces cerevisiae (SGSC) on chickens, two experiments were researched. In vitro experiment, the effects of SGSC on chicken splenic lymphocyte proliferation were determined. The results displayed that SGSC could significantly stimulate chicken splenic lymphocyte proliferation. In vivo experiment, 200 14-day-old chickens were averagely divided into 5 groups. The chickens, except blank control (BC) group, were vaccinated with Newcastle disease (ND) vaccine, repeated vaccination at 28 days old. At the same time of the first vaccination, the chickens in three SGSC groups were injected, respectively, with the SGSC at low, medium and high concentrations, in vaccination control (VC) and BC group, with equal volume of physiological saline, once a day for three successive days. On days 7, 14, 21, 28, 35 and 42 after the first vaccination, the lymphocyte proliferation, serum antibody titer and interleukin-2 (IL-2) and interferon-gamma (IFN-γ) were measured. The results showed that SGSC at suitable dose could significantly promote lymphocyte proliferation, enhance serum antibody titer, and improve serum IL-2 and IFN-γ concentrations. It indicated that SGSC could significantly improve the immune efficacy of Newcastle disease vaccine, and would be as the candidate of a new-type immune adjuvant.
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China; Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: wangmi@shvri.ac.cn.
authors.forename: Mi
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Ruile
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Chenzhong
authors.initials: C
authors.lastname: Fei
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Keyu
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Sui
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Saiqi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. Electronic address: feiqun@gmail.com.
authors.forename: Feiqun
authors.initials: F
authors.lastname: Xue
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: ylhu@njau.edu.cn.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002645:Chickens; D005936:Glucans / Q000737:chemistry / Q000276:immunology*; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000276:immunology* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D005936:Glucans; D007376:Interleukin-2; D014765:Viral Vaccines; C078962:glucan sulfate; D007371:Interferon-gamma
keywords: Cytokines; Lymphocyte proliferation; Newcastle disease vaccine; Serum antibody titer; Sulfated glucan from Saccharomyces cerevisiae