title: Ergosterol reverses multidrug resistance in SGC7901/Adr cells.
abstract: Multidrug resistance (MDR) is a major obstacle in the chemotherapeutic treatment of tumors. Elevated expression of the P-glycoprotein (P-gp) transporter is associated with MDR and responsible for the resistance of tumor cells against a variety of anticancer drugs. In this study, the reversal effect of ergosterol (Erg) on SGC7901/Adr cells was investigated. At concentrations of 1 microM and 5 microM, Erg could reverse the resistance of SGC7901/Adr to adriamycin up to 4.84 and 3.92 folds, respectively. Mechanistically, Erg could increase the intracellular accumulation of adriamycin and Rh123 in SGC7901/Adr cells through inhibiting the transcription of MDR1 gene and down-regulating the expression of P-gp. In conclusion, Erg could reverse the MDR of SGC7901/Adr cells via its influence on P-gp expression and thus be a promising lead compound for future studies.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Hu
authors.forename: Danhong
authors.initials: D
authors.lastname: Jiang
authors.forename: Farong
authors.initials: F
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.forename: Yaya
authors.initials: Y
authors.lastname: Huang
authors.forename: Shuanghong
authors.initials: S
authors.lastname: Song
authors.forename: Zhezhi
authors.initials: Z
authors.lastname: Wang
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis / Q000235:genetics; D000903:Antibiotics, Antineoplastic / Q000378:metabolism; D045744:Cell Line, Tumor; D004396:Coloring Agents; D004317:Doxorubicin / Q000378:metabolism; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D004875:Ergosterol / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D020112:Rhodamine 123; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000903:Antibiotics, Antineoplastic; D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D020112:Rhodamine 123; D004317:Doxorubicin; C022616:thiazolyl blue; D004875:Ergosterol