title: Effect of visible light on catalytic hydrolysis of p-nitrophenyl palmitate by the Pseudomonas cepacia lipase immobilized on sol-gel support.
abstract: This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250-750 nm. The reaction follows Michaelis-Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver-Burk plot K M and V max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction.
authors.affiliation: Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, Gambang, 26300, Kuantan, Pahang Darul Makmur, Malaysia.
authors.forename: Ponnarasy
authors.initials: P
authors.lastname: Ganasen
authors.forename: Maksudur Rahman
authors.initials: MR
authors.lastname: Khan
authors.forename: M Abul
authors.initials: MA
authors.lastname: Kalam
authors.forename: Mohd Sabri
authors.initials: MS
authors.lastname: Mahmud
mesh_terms: D057005:Bioengineering; D016956:Burkholderia cepacia / Q000201:enzymology*; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism* / Q000528:radiation effects; D006868:Hydrolysis; D007700:Kinetics; D008027:Light; D008049:Lipase / Q000378:metabolism* / Q000528:radiation effects; D053858:Metabolic Networks and Pathways; D010168:Palmitates / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D010168:Palmitates; C050015:4-nitrophenyl palmitate; D008049:Lipase