title: Reprint of: keratin intermediate filaments: differences in the sequences of the Type I and Type II chains explain the origin of the stability of an enzyme-resistant four-chain fragment.
abstract: Previous studies have shown that a strong interaction exists between oppositely directed 1B molecular segments in the intermediate filaments of trichocyte keratins. A similar interaction has been identified as having a significant role in the formation of unit-length filaments, a precursor to intermediate filament formation. The present study is concerned with the spatial relationship of these interacting segments and its dependence on differences in the amino acid sequences of the two-chain regions that constitute the 1B molecular segment. It is shown that along a particular line of contact both chain segments possess an elevated concentration of residues with a high propensity for dimer formation. The transition from the reduced to the oxidized state involves a simple axial displacement of one molecular segment relative to the other, with no attendant rotation of either segment. This changes the inter-relationship of the two 1B molecular segments from a loosely packed form to a more compact one. After the slippage eight of the cysteine residues in the dimer are precisely aligned to link up and form the disulfide linkages as observed. The two remaining cysteine residues are located on the outside of the dimer and are presumably involved in inter-dimer bonding. The existence of a unique line of contact requires that two chains in the molecule have different amino acid compositions with the clustering of dimer-favoring residues phased by half the pitch length of the coiled coil.
authors.affiliation: Institute of Fundamental Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand. Electronic address: b-fraser@bigpond.net.au.
authors.forename: R D Bruce
authors.initials: RD
authors.lastname: Fraser
authors.affiliation: Institute of Fundamental Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand; Riddet Institute, Massey University, Private Bag 11-222, Palmerston North, New Zealand. Electronic address: d.parry@massey.ac.nz.
authors.forename: David A D
authors.initials: DA
authors.lastname: Parry
keywords: Coiled coil packing; Disulfide bonds; Hydropathy; Trichocyte keratin; Vimentin