title: Effects of canonical NF-κB signaling pathway on the proliferation and odonto/osteogenic differentiation of human stem cells from apical papilla.
abstract: BACKGROUND
  NF-κB signaling pathway plays a complicated role in the biological functions of mesenchymal stem cells. However, the effects of NF-κB pathway on the odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs) remain unclear. The present study was designed to evaluate the effects of canonical NF-κB pathway on the osteo/odontogenic capacity of SCAPs in vitro.
  
  
  RESULTS
  Western blot results demonstrated that NF-κB pathway in SCAPs was successfully activated by TNF-α or blocked by BMS-345541. NF-κB pathway-activated SCAPs presented a higher proliferation activity compared with control groups, as indicated by dimethyl-thiazol-diphenyl tetrazolium bromide assay (MTT) and flow cytometry assay (FCM). Wound scratch assay revealed that NF-κB pathway-activated SCAPs presented an improved migration capacity, enhanced alkaline phosphatase (ALP) activity, and upregulated mineralization capacity of SCAPs, as compared with control groups. Meanwhile, the odonto/osteogenic markers (ALP/ALP, RUNX2/RUNX2, OSX/OSX, OCN/OCN, OPN/OPN, BSP/BSP, DSPP/DSP, and DMP-1/DMP-1) in NF-κB pathway-activated SCAPs were also significantly upregulated as compared with control groups at both protein and mRNA levels. However, NF-κB pathway-inhibited SCAPs exhibited a lower proliferation/migration capacity, and decreased odonto/osteogenic ability in comparison with control groups.
  
  
  CONCLUSIONS
  Our findings suggest that classical NF-κB pathway plays a paramount role in the proliferation and committed differentiation of SCAPs.
authors.affiliation: Institute of Stomatology, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu 210029, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Li
authors.affiliation: Endodontic Department, School of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, Jiangsu 210029, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.affiliation: Institute of Stomatology, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu 210029, China.
authors.forename: Zilu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Endodontic Department, School of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, Jiangsu 210029, China.
authors.forename: Shuanglin
authors.initials: S
authors.lastname: Jing
authors.affiliation: Institute of Stomatology, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu 210029, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Stomatology, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu 210029, China.
authors.forename: Genxia
authors.initials: G
authors.lastname: Liu
authors.affiliation: Institute of Stomatology, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu 210029, China ; Endodontic Department, School of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, Jiangsu 210029, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Laboratory of Molecular Signaling and Stem Cells Therapy and Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, 4 Tian Tan Xi Li, Beijing 100050, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Fan
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D003771:Dental Papilla / Q000166:cytology*; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D009805:Odontogenesis; D010012:Osteogenesis; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology*
chemical_list: D016328:NF-kappa B