title: Ameliorative effect of vanadium on oxidative stress in stomach tissue of diabetic rats.
abstract: Between their broad spectrum of action, vanadium compounds are shown to have insulin mimetic/enhancing effects. Increasing evidence in experimental and clinical studies suggests that oxidative stress plays a major role in the pathogenesis of diabetes and on the onset of diabetic complications. Thus, preventive therapy can alleviate the possible side effects of the disease. The aim of the present study was to investigate the effect of vanadyl sulfate supplementation on the antioxidant system in the stomach tissue of diabetic rats. Male Swiss albino rats were randomly divided into 4 groups: control; control+vanadyl sulfate; diabetic; diabetic+vanadyl sulfate. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ; 65 mg/kg body weight). Vanadyl sulfate (100 mg/kg body weight) was given daily by gavage for 60 days. At the last day of the experiment, stomach tissues were taken and homogenized to make a 10% (w/v) homogenate. Catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), myeloperoxidase (MPO), carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PD) and lactate dehydrogenase (LDH) activities were determined in the stomach tissue. CAT, SOD, GR, GPx, GST, CA, G6PD and LDH activities were increased in diabetic rats when compared to normal rats. Vanadium treatment significantly reduced the elevated activities of GR, GPx, GST compared with the diabetic group whereas the decreases in CAT, SOD, CA, G6PD and LDH activities were insignificant. No significant change was seen for MPO activity between the groups. It was concluded that vanadium could be used for its ameliorative effect against oxidative stress in diabetes.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Istanbul University, Beyazit, 34116, Istanbul, Turkey.
authors.forename: Tugba
authors.initials: T
authors.lastname: Yilmaz-Ozden
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Istanbul University, Beyazit, 34116, Istanbul, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Kurt-Sirin
authors.affiliation: Department of Chemistry, Faculty of Engineering, Istanbul University, Avcılar, 34320, Istanbul, Turkey.
authors.forename: Sevim
authors.initials: S
authors.lastname: Tunali
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Istanbul University, Beyazit, 34116, Istanbul, Turkey.
authors.forename: Nuriye
authors.initials: N
authors.lastname: Akev
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Istanbul University, Beyazit, 34116, Istanbul, Turkey.
authors.forename: Ayse
authors.initials: A
authors.lastname: Can
authors.affiliation: Department of Chemistry, Faculty of Engineering, Istanbul University, Avcılar, 34320, Istanbul, Turkey.
authors.forename: Refiye
authors.initials: R
authors.lastname: Yanardag
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D013270:Stomach / Q000187:drug effects* / Q000201:enzymology; D017968:Vanadium Compounds / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D007004:Hypoglycemic Agents; D017968:Vanadium Compounds; C034028:vanadyl sulfate; D010088:Oxidoreductases