title: Kinetics of oxygen uptake by cells potentially used in a tissue engineered trachea.
abstract: Synthetic polymer scaffold seeded with autologous cells have a clinical translational potential. A rational design oriented to clinical applications must ensure an efficient mass transfer of nutrients as a function of specific metabolic rates, especially for precariously vascularized tissues grown in vitro or integrated in vivo. In this work, luminescence lifetime-based sensors were used to provide accurate, extensive and non-invasive measurements of the oxygen uptake rate for human mesenchymal stem cells (hMSCs), tracheal epithelial cells (hTEpiCs) and human chondrocytes (hCCs) within a range of 2-40% O2 partial pressure. Estimated Michaelis-Menten parameters were: V(max) = 0.099 pmol/cell⋅h and K(M) = 2.12 × 10(-7) mol/cm(3) for hMSCs, V(max) = 1.23 pmol/cell⋅h and K(M) = 2.14 × 10(-7) mol/cm(3) for hTEpiCs, V(max) = 0.515 pmol/cell⋅h and K(M) = 1.65 × 10(-7) mol/cm(3) for hCCs. Kinetics data served as an input to a preliminary computational simulation of cell culture on a poly-ethylene terephthalate (PET) tracheal scaffold obtaining an efficient mass transfer at cell density of 10(6) cell/cm(3). Oxygen concentration affected the glucose uptake and lactate production rates of cells that adapted their metabolism according to energy demand in hypoxic and normoxic conditions.
authors.affiliation: Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci Cubo 17/C, I-87030 Rende (CS), Italy; Department of Environmental and Chemical Engineering, University of Calabria, (DIATIC-UNICAL), via P. Bucci Cubo 45A, 87036 Rende (CS), Italy.
authors.forename: Efrem
authors.initials: E
authors.lastname: Curcio
authors.affiliation: Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci Cubo 17/C, I-87030 Rende (CS), Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Piscioneri
authors.affiliation: Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci Cubo 17/C, I-87030 Rende (CS), Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Morelli
authors.affiliation: Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci Cubo 17/C, I-87030 Rende (CS), Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Salerno
authors.affiliation: Advanced Center for Translational Regenerative Medicine (ACTREM), Karolinska Institutet, Huddinge SE-14186, Stockholm, Sweden.
authors.forename: Paolo
authors.initials: P
authors.lastname: Macchiarini
authors.affiliation: Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci Cubo 17/C, I-87030 Rende (CS), Italy. Electronic address: l.debartolo@itm.cnr.it.
authors.forename: Loredana
authors.initials: L
authors.lastname: De Bartolo
mesh_terms: D002452:Cell Count; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000737:chemistry / Q000166:cytology; D003198:Computer Simulation; D004847:Epithelial Cells / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D059630:Mesenchymal Stem Cells; D008962:Models, Theoretical; D010100:Oxygen / Q000378:metabolism*; D011093:Polyethylene Terephthalates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014132:Trachea / Q000737:chemistry* / Q000166:cytology
chemical_list: D011093:Polyethylene Terephthalates; D011108:Polymers; D019344:Lactic Acid; D010100:Oxygen
keywords: Mass transfer; Oxygen uptake rate; Tissue engineered airway; Tracheal cells; Tracheal scaffold