title: Flowering as the most highly sensitive period of grapevine (Vitis vinifera L. cv Mourvèdre) to the Botryosphaeria dieback agents Neofusicoccum parvum and Diplodia seriata infection.
abstract: Botryosphaeria dieback is a fungal grapevine trunk disease that currently represents a threat for viticulture worldwide because of the important economical losses due to reduced yield of affected plants and their premature death. Neofusicoccum parvum and Diplodia seriata are among the causal agents. Vine green stems were artificially infected with N. parvum or D. seriata at the onset of three different phenological stages (G stage (separated clusters), flowering and veraison). Highest mean lesion lengths were recorded at flowering. Major proteome changes associated to artificial infections during the three different phenological stages were also reported using two dimensional gel electrophoresis (2D)-based analysis. Twenty (G stage), 15 (flowering) and 13 (veraison) differentially expressed protein spots were subjected to nanoLC-MS/MS and a total of 247, 54 and 25 proteins were respectively identified. At flowering, a weaker response to the infection was likely activated as compared to the other stages, and some defense-related proteins were even down regulated (e.g., superoxide dismutase, major latex-like protein, and pathogenesis related protein 10). Globally, the flowering period seemed to represent the period of highest sensitivity of grapevine to Botryosphaeria dieback agent infection, possibly being related to the high metabolic activity in the inflorescences.
authors.affiliation: Université de Reims Champagne-Ardenne, URVVC EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, Reims (Cedex 2) 51687, France. alessandro.spagnolo@univ-reims.fr.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Spagnolo
authors.affiliation: Institut Français de la Vigne et du Vin Pôle Rhône-Méditerranée, France, Domaine de Donadille, Rodilhan 30230, France. Philippe.LARIGNON@vignevin.com.
authors.forename: Philippe
authors.initials: P
authors.lastname: Larignon
authors.affiliation: Université de Reims Champagne-Ardenne, URVVC EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, Reims (Cedex 2) 51687, France. maryline.magnin-robert@univ-reims.fr.
authors.forename: Maryline
authors.initials: M
authors.lastname: Magnin-Robert
authors.affiliation: Université de Strasbourg, IPHC, UMR 7178, Laboratoire de Spectrométrie de Masse Bioorganique, Strasbourg 67087, France. ahovasse@unistra.fr.
authors.forename: Agnès
authors.initials: A
authors.lastname: Hovasse
authors.affiliation: Université de Reims Champagne-Ardenne, URVVC EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, Reims (Cedex 2) 51687, France. christophe.clement@univ-reims.fr.
authors.forename: Clara
authors.initials: C
authors.lastname: Cilindre
authors.affiliation: Université de Strasbourg, IPHC, UMR 7178, Laboratoire de Spectrométrie de Masse Bioorganique, Strasbourg 67087, France. alain.vandorsselaer@unistrat.fr.
authors.forename: Alain
authors.initials: A
authors.lastname: Van Dorsselaer
authors.affiliation: Université de Reims Champagne-Ardenne, URVVC EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, Reims (Cedex 2) 51687, France. christophe.clement@univ-reims.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Clément
authors.affiliation: Université de Strasbourg, IPHC, UMR 7178, Laboratoire de Spectrométrie de Masse Bioorganique, Strasbourg 67087, France. christine.schaeffer@unistra.fr.
authors.forename: Christine
authors.initials: C
authors.lastname: Schaeffer-Reiss
authors.affiliation: Université de Reims Champagne-Ardenne, URVVC EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, Reims (Cedex 2) 51687, France. florence.fontaine@univ-reims.fr.
authors.forename: Florence
authors.initials: F
authors.lastname: Fontaine
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D027843:Vitis / Q000254:growth & development / Q000382:microbiology* / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D020543:Proteome