title: Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells.
abstract: Stem cell-based therapies have drawn intensive attention in the neuronal regenerative fields. Several studies have revealed that stem cells can serve as an inexhaustible source for neurons for transplantation therapies. However, generation of neurons and directionality has not yet been fully investigated. Herein, we investigate the mechanical ramifications of surface topography on human embryonic cell differentiation. Microgrooved surfaces with various pitches were applied to modulate the neuron differentiation. Our protocol showed that neuron differentiation increased as grove pitch decreased. The results indicated that 2 μm microgrooves can improve neuron growth by ~1.7-fold. Our results indicate the importance of mechanotransduction on neuronal differentiation and highlight the feasibility of manipulating the neuronal differentiation with surface topography, providing new perspectives for accommodating clinical transplantation.
authors.affiliation: Bioengineering, University of California, Merced, CA, USA.
authors.forename: David
authors.initials: D
authors.lastname: Lu
authors.forename: Chi-Shuo
authors.initials: CS
authors.lastname: Chen
authors.forename: Chao-Sung
authors.initials: CS
authors.lastname: Lai
authors.forename: Sushant
authors.initials: S
authors.lastname: Soni
authors.forename: Teranze
authors.initials: T
authors.lastname: Lam
authors.forename: Taranze
authors.initials: T
authors.lastname: Lam
authors.forename: Clarence
authors.initials: C
authors.lastname: Le
authors.forename: Eric Y-T
authors.initials: EY
authors.lastname: Chen
authors.forename: Thien
authors.initials: T
authors.lastname: Nguyen
authors.forename: Wei-Chun
authors.initials: WC
authors.lastname: Chin
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D002645:Chickens; D020219:Chondrogenesis / Q000187:drug effects; D058732:Embryoid Bodies / Q000166:cytology; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D013499:Surface Properties; D034421:Sus scrofa; D054457:Tissue Scaffolds
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate