title: MC3T3-E1 cell response of amorphous phase/TiO2 nanocrystal composite coating prepared by microarc oxidation on titanium.
abstract: Bioactive amorphous phase/TiO2 nanocrystal (APTN) composite coatings were fabricated by microarc oxidation (MAO) on Ti. The APTN coatings are composed of much amorphous phase with Si, Na, Ca, Ti and O elements and a few TiO2 nanocrystals. With increasing applied voltage, the micropore density of the APTN coating decreases and the micropore size of the APTN coating increases. The results indicate that less MC3T3-E1 cells attach on the APTN coatings as compared to Ti. However, the APTN coatings greatly enhance the cell proliferation ability and the activity of alkaline phosphatase. The amorphous phase and the concentrations of the released Ca and Si from the APTN coatings during cell culture have significant effects on the cell response.
authors.affiliation: Department of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Department of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China. Electronic address: daqingwei@hit.edu.cn.
authors.forename: Daqing
authors.initials: D
authors.lastname: Wei
authors.affiliation: Department of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
authors.forename: Haoyue
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.affiliation: Department of Mechanical Engineering, School of Architecture and Civil Engineering, Harbin University of Science and Technology, Harbin 150001, PR China.
authors.forename: Su
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Department of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
authors.forename: Baoqiang
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
authors.forename: Yaming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
authors.forename: Dechang
authors.initials: D
authors.lastname: Jia
authors.affiliation: Department of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Amorphous phase; MC3T3-E1 cells; Microarc oxidation; Microstructure; Titanium