title: Oxidative stress of office workers relevant to tobacco smoking and inner air quality.
abstract: Studies have used 8-hydroxydeoxyguanosine (8-OHdG) as a biomarker to detect systemic oxidative DNA damage associated with oxidative stress. However, studies on the association between exposure to tobacco smoking and urinary 8-OHdgG give inconsistent results. Limited studies have estimated the oxidative stress among office workers. This study assessed the association between urinary 8-OHdG and cotinine for office workers.  Workers (389) including smokers, ex-smokers and non-smokers from 87 offices at high-rise buildings in Taipei participated in this study with informed consent. Each participant completed a questionnaire and provided a spot urine specimen at the end of work day for measuring urinary 8-OHdG and cotinine. The carbon dioxide (CO2) levels in workers' offices were also measured. The questionnaire reported socio-demographic characteristics, life styles and allergic history. The urinary 8-OHdG level increased with the cotinine level among participants (Spearmans' rho = 0.543, p < 0.001). The mean of urinary 8-OHdG and cotinine was 5.81 ± 3.53 μg/g creatinine and 3.76 ± 4.06 μg/g creatinine, respectively. Comparing with non-smokers, the adjusted odds ratio (OR) of having urinary 8-OHdG greater than the median level of 4.99 μg/g creatinine was 5.30 (95% confidence intervals (CI) = 1.30-21.5) for current smokers and 0.91 (95% CI = 0.34-2.43) for former smokers. We also found workers exposed to 1,000 ppm of CO2 at offices had an adjusted OR of 4.28 (95% CI = 1.12-16.4) to have urinary 8-OHdG greater than 4.99 μg/g creatinine, compared to those exposed to indoor CO2 under 600 ppm. In conclusion, urinary 8-OHdG could represent a suitable marker for measuring smoking and CO2 exposure for office workers.
authors.affiliation: School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung 404, Taiwan. u100030082@cmu.edu.tw.
authors.forename: Chung-Yen
authors.initials: CY
authors.lastname: Lu
authors.affiliation: Institute of Environmental Health, National Taiwan University, Taipei 100, Taiwan. ycma@ntu.edu.tw.
authors.forename: Yee-Chung
authors.initials: YC
authors.lastname: Ma
authors.affiliation: Institute of Epidemiology and Preventive Medicine, National Taiwan University, Taipei 100, Taiwan. peichunchen@ntu.edu.tw.
authors.forename: Pei-Chun
authors.initials: PC
authors.lastname: Chen
authors.affiliation: Department of Public Health, China Medical University, Taichung 404, Taiwan. wucc@mail.cmu.edu.tw.
authors.forename: Chin-Ching
authors.initials: CC
authors.lastname: Wu
authors.affiliation: Department of Health Management, I-Shou University, Kaohsiung 824, Taiwan. kimi@isu.edu.tw.
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Chen
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects / Q000032:analysis*; D015415:Biomarkers / Q000652:urine; D002681:China; D003367:Cotinine / Q000652:urine*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D018384:Oxidative Stress / Q000187:drug effects*; D011795:Surveys and Questionnaires; D014028:Tobacco Smoke Pollution / Q000009:adverse effects / Q000032:analysis*
chemical_list: D000395:Air Pollutants, Occupational; D015415:Biomarkers; D014028:Tobacco Smoke Pollution; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D003367:Cotinine