title: Nanocrystalline hydroxyapatite doped with selenium oxyanions: a new material for potential biomedical applications.
abstract: Selenium-substituted hydroxyapatites containing selenate SeO4(2-) or selenite SeO3(2-) ions were synthesized using a wet precipitation method. The selenium content was determined by atomic absorbance spectrometry. The raw, unsintered powders were also characterized using powder X-ray diffraction, middle-range FT-IR spectroscopy and transmission electron microscopy with energy-dispersive X-ray spectroscopic microanalysis. The synthesized apatites were found to be pure and nanocrystalline with a crystal size similar to that in bone mineral. The incorporation of selenium oxyanions into the crystal lattice was confirmed. The toxicity of hydroxyapatites containing selenite or selenate ions was evaluated with a protozoan assay and bacterial luminescence test.
authors.affiliation: Medical University of Warsaw, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry, ul. Banacha 1, 02-097 Warsaw, Poland. Electronic address: joanna.kolmas@wum.edu.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kolmas
authors.affiliation: Medical University of Warsaw, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry, ul. Banacha 1, 02-097 Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Oledzka
authors.affiliation: Medical University of Warsaw, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry, ul. Banacha 1, 02-097 Warsaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Sobczak
authors.affiliation: Medical University of Warsaw, Faculty of Pharmacy, Department of Environmental Health Sciences, ul. Banacha 1, 02-097 Warsaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Nałęcz-Jawecki
mesh_terms: D048908:Aliivibrio fischeri / Q000187:drug effects / Q000302:isolation & purification; D001672:Biocompatible Materials / Q000737:chemistry*; D020811:Biomedical Technology; D018786:Bone Substitutes / Q000138:chemical synthesis; D016798:Ciliophora / Q000187:drug effects / Q000302:isolation & purification; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D011208:Powders; D012643:Selenium / Q000737:chemistry* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis; D018675:Toxicity Tests; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D011208:Powders; D017886:Durapatite; D012643:Selenium
keywords: Biomaterials; FT-IR; Nanocrystalline hydroxyapatite; Selenium; TEM; XRD