title: Quality control of ion torrent sequencing library.
abstract: Next-generation sequencing (NSG) is an important method for gathering large amounts of sequencing data for different types of applications regarding the diagnosis and response to treatment of different diseases. An important step in the NGS process is the quality control of sequencing libraries, which can influence the yield and efficiency of the sequencing run. This study evaluated two different methods for library quality control, Agilent Bioanalyzer and qPCR, and showed that both methods can be used. However, as is the case with any analytical method, they have their limitations. The Agilent Bioanalyzer quantifies only the high quality libraries, but it underestimates their concentration, while qPCR also quantifies lower quality libraries, but it overestimates their concentration.
authors.affiliation: The Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Laura-Ancuţa
authors.initials: LA
authors.lastname: Pop
authors.affiliation: Department of Surgery before "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Surgical Oncology, The Oncology Institute ``Prof. Dr. Ion Chiricuta'', Cluj-Napoca, Romania.
authors.forename: Emil
authors.initials: E
authors.lastname: Puscas
authors.affiliation: The Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Analytical Chemistry, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Valentina
authors.initials: V
authors.lastname: Pileczki
authors.affiliation: The Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Experimental Biology, Faculty of Biology and Geology, "Babes Bolyai" University, Cluj-Napoca, Romania.
authors.forename: Roxana
authors.initials: R
authors.lastname: Cojocneanu-Petric
authors.affiliation: The Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Functional Genomics and Experimental Pathology, The Oncology Institute ``Prof. Dr. Ion Chiricuta'', Cluj-Napoca, Romania.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Braicu
authors.affiliation: Department of Surgical Oncology, The Oncology Institute ``Prof. Dr. Ion Chiricuta'', Cluj-Napoca, Romania Department of Surgical Oncolgy and Gynecological Oncology Iuliu Hatieganu University of Medicine and Pharmacy Iuliu Hatieganu, Cluj Napoca, Romania.
authors.forename: Patriciu
authors.initials: P
authors.lastname: Achimas-Cadariu
authors.affiliation: The Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Functional Genomics and Experimental Pathology, The Oncology Institute ``Prof. Dr. Ion Chiricuta'', Cluj-Napoca, Romania Department of Immunology Iuliu Hatieganu University of Medicine and Pharmacy Cluj Napoca, Romania.
authors.forename: Ioana
authors.initials: I
authors.lastname: Berindan-Neagoe
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D005260:Female; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D011786:Quality Control; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods
keywords: Agilent Bioanalyzer; breast cancer; qPCR; sequencing libraries