title: Isolation and identification of antitrypanosomal and antimycobacterial active steroids from the sponge Haliclona simulans.
abstract: The marine sponge Haliclona simulans collected from the Irish Sea yielded two new steroids: 24-vinyl-cholest-9-ene-3β,24-diol and 20-methyl-pregn-6-en-3β-ol,5a,8a-epidioxy, along with the widely distributed 24-methylenecholesterol. One of the steroids possesses an unusually short hydrocarbon side chain. The structures were elucidated using nuclear magnetic resonance spectroscopy and confirmed using electron impact- and high resolution electrospray-mass spectrometry. All three steroids possess antitrypanosomal and anti-mycobacterial activity. All the steroids were found to possess low cytotoxicity against Hs27 which was above their detected antitrypanosomal potent concentrations.
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 161 Cathedral Street, Glasgow, Scotland G4 0RE, UK. christina.viegelmann@strath.ac.uk.
authors.forename: Christina
authors.initials: C
authors.lastname: Viegelmann
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 161 Cathedral Street, Glasgow, Scotland G4 0RE, UK. jennifer.parker.100@strath.ac.uk.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Parker
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 161 Cathedral Street, Glasgow, Scotland G4 0RE, UK. thengthengooi@hotmail.com.
authors.forename: Thengtheng
authors.initials: T
authors.lastname: Ooi
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 161 Cathedral Street, Glasgow, Scotland G4 0RE, UK. c.j.clements@strath.ac.uk.
authors.forename: Carol
authors.initials: C
authors.lastname: Clements
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 161 Cathedral Street, Glasgow, Scotland G4 0RE, UK. grainne.abbott@strath.ac.uk.
authors.forename: Gráinne
authors.initials: G
authors.lastname: Abbott
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 161 Cathedral Street, Glasgow, Scotland G4 0RE, UK. louise.c.young@strath.ac.uk.
authors.forename: Louise
authors.initials: L
authors.lastname: Young
authors.affiliation: Marine Biotechnology Centre, Environmental Research Institute, University College Cork, Lee Road, Cork, Ireland. jonathan.kennedy@ucc.ie.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Kennedy
authors.affiliation: Marine Biotechnology Centre, Environmental Research Institute, University College Cork, Lee Road, Cork, Ireland. A.Dobson@ucc.ie.
authors.forename: Alan D W
authors.initials: AD
authors.lastname: Dobson
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 161 Cathedral Street, Glasgow, Scotland G4 0RE, UK. ruangelie.edrada-ebel@strath.ac.uk.
authors.forename: RuAngelie
authors.initials: R
authors.lastname: Edrada-Ebel
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006888:Hydroxycholesterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008968:Molecular Conformation; D009161:Mycobacterium / Q000187:drug effects*; D011161:Porifera / Q000737:chemistry*; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014344:Trypanocidal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014345:Trypanosoma / Q000187:drug effects
chemical_list: C000593629:20-methyl-pregn-6-en-3beta-ol,5a,8a-epidioxy; C000593628:24-vinyl-cholest-9-ene-3beta,24-diol; D000900:Anti-Bacterial Agents; D006888:Hydroxycholesterols; D011278:Pregnanes; D014344:Trypanocidal Agents