title: Pharmacological assessment of methamphetamine-induced behavioral hyperactivity mediated by dopaminergic transmission in planarian Dugesia japonica.
abstract: The freshwater planarian Dugesia japonica has a simple central nervous system (CNS) and can regenerate complete organs, even a functional brain. Recent studies demonstrated that there is a great variety of neuronal-related genes, specifically expressed in several domains of the planarian brain. We identified a planarian dat gene, named it D. japonica dopamine transporter (Djdat), and analyzed its expression and function. Both in situ hybridization and immunofluorescence revealed that localization of Djdat mRNA and protein was the same as that of D. japonica tyrosine hydroxylase (DjTH). Although, dopamine (DA) content in Djdat(RNAi) planarians was not altered, Djdat(RNAi) planarians showed increased spontaneous locomotion. The hyperactivity in the Djdat(RNAi) planarians was significantly suppressed by SCH23390 or sulpiride pretreatment, which are D1 or D2 receptor antagonists, respectively. These results suggest that planarians have a Djdat ortholog and the ability to regulate dopaminergic neurotransmission and association with spontaneous locomotion.
authors.affiliation: Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Natsuka
authors.initials: N
authors.lastname: Tashiro
authors.affiliation: Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan; Department of Biophysics, Graduate School of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Kaneyasu
authors.initials: K
authors.lastname: Nishimura
authors.affiliation: Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Daido
authors.affiliation: Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Tomoe
authors.initials: T
authors.lastname: Oka
authors.affiliation: Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Mio
authors.initials: M
authors.lastname: Todo
authors.affiliation: Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Asami
authors.initials: A
authors.lastname: Toshikawa
authors.affiliation: Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Tsushima
authors.affiliation: Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Takata
authors.affiliation: Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Eishi
authors.initials: E
authors.lastname: Ashihara
authors.affiliation: Department of Food Sciences and Biotechnology, Faculty of Life Science, Hiroshima Institute of Technology, 2-1-1 Miyake, Saeki-ku, Hiroshima 731-5193, Japan.
authors.forename: Kanji
authors.initials: K
authors.lastname: Yoshimoto
authors.affiliation: Department of Biophysics, Graduate School of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Kiyokazu
authors.initials: K
authors.lastname: Agata
authors.affiliation: Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan. Electronic address: yo-kita@mb.kyoto-phu.ac.jp.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D002490:Central Nervous System / Q000378:metabolism; D004298:Dopamine / Q000502:physiology*; D065127:Dopamine D2 Receptor Antagonists; D050483:Dopamine Plasma Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics; D008124:Locomotion / Q000187:drug effects; D008694:Methamphetamine / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000235:genetics; D010932:Planarians; D017447:Receptors, Dopamine D1 / Q000037:antagonists & inhibitors; D016415:Sequence Alignment; D013469:Sulpiride / Q000494:pharmacology; D009435:Synaptic Transmission / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D001552:Benzazepines; D065127:Dopamine D2 Receptor Antagonists; D050483:Dopamine Plasma Membrane Transport Proteins; D017447:Receptors, Dopamine D1; C534628:SCH 23390; D008694:Methamphetamine; D013469:Sulpiride; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine
keywords: Dopamine transporter; Methamphetamine; Planarian; Spontaneous locomotion