title: A study of the dexamethasone sodium phosphate release properties from a periocular capsular drug delivery system.
abstract: The aim of this study was to investigate whether a periocular capsular drug delivery system (DDS) can release dexamethasone sodium phosphate (DEXP) in vitro and in vivo to the posterior segment of rabbit's eye. In vitro, the periocular capsular DDS containing 2 mg/ml or 5 mg/ml DEXP was immersed in modified Franz diffusion cell. Four-hundred microliters of liquid was aspirated at 0.5, 1, 2, 4, 8, 24 and 48 h for determination. In vivo, the DEXP-filled periocular capsular DDS was implanted into the sub-Tenon's sac of the New Zealand rabbit. DEXP concentration at the serum aqueous humor, cornea, iris, lens, ciliary body, vitreous, retina, choroids and sclera was quantified at 1, 3, 7, 14, 28 and 56 d after implantation. The DEXP concentration was determined by ultra-performance liquid chromatography-tandem mass spectrometry. In vitro, the periocular capsular DDS released the DEXP in time-dependent manner from 1/2 to 48 h. In vivo, the concentrations of the DEXP at the retina, choroids, ciliary body and iris were 123.11 (91.23, 732.61) ng/g, 362.46 ± 330.46 ng/g, 71.64 (71.35, 180.21) ng/g and 192.50 ± 42.66 ng/g, respectively, at 56 d after implantation. Minimal DEXP was found in the aqueous, serum and vitreous. Our results demonstrated that DEXP could be sustained released from the periocular capsular DDS, which indicated that the periocular capsular DDS might be a potential candidate of transscleral drug delivery for the management of posterior segment diseases.
authors.affiliation: a State Key Laboratory of Ophthalmology , Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou , People's Republic of China and.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
authors.affiliation: a State Key Laboratory of Ophthalmology , Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou , People's Republic of China and.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: a State Key Laboratory of Ophthalmology , Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou , People's Republic of China and.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zong
authors.affiliation: a State Key Laboratory of Ophthalmology , Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou , People's Republic of China and.
authors.forename: Suo
authors.initials: S
authors.lastname: Qiu
authors.affiliation: a State Key Laboratory of Ophthalmology , Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou , People's Republic of China and.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chen
authors.affiliation: a State Key Laboratory of Ophthalmology , Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou , People's Republic of China and.
authors.forename: Xuyuan
authors.initials: X
authors.lastname: Sun
authors.affiliation: b Laboratory of Pharmaceutical Analysis and Quality Assessment , School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou , People's Republic of China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: b Laboratory of Pharmaceutical Analysis and Quality Assessment , School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou , People's Republic of China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Xie
authors.affiliation: a State Key Laboratory of Ophthalmology , Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou , People's Republic of China and.
authors.forename: Qianying
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000818:Animals; D000869:Anterior Eye Segment / Q000378:metabolism; D003692:Delayed-Action Preparations / Q000008:administration & dosage; D003907:Dexamethasone / Q000008:administration & dosage / Q000031:analogs & derivatives*; D016503:Drug Delivery Systems / Q000379:methods; D011817:Rabbits
chemical_list: D003692:Delayed-Action Preparations; C004180:dexamethasone 21-phosphate; D003907:Dexamethasone
keywords: Drug delivery; drug distribution; eye; rabbit; ultra-performance liquid chromatography-tandem mass spectrometry