title: Mass spectrometry-based N-glycoproteomics for cancer biomarker discovery.
abstract: Glycosylation is estimated to be found in over 50% of human proteins. Aberrant protein glycosylation and alteration of glycans are closely related to many diseases. More than half of the cancer biomarkers are glycosylated-proteins, and specific glycoforms of glycosylated-proteins may serve as biomarkers for either the early detection of disease or the evaluation of therapeutic efficacy for treatment of diseases. Glycoproteomics, therefore, becomes an emerging field that can make unique contributions to the discovery of biomarkers of cancers. The recent advances in mass spectrometry (MS)-based glycoproteomics, which can analyze thousands of glycosylated-proteins in a single experiment, have shown great promise for this purpose. Herein, we described the MS-based strategies that are available for glycoproteomics, and discussed the sensitivity and high throughput in both qualitative and quantitative manners. The discovery of glycosylated-proteins as biomarkers in some representative diseases by employing glycoproteomics was also summarized.
authors.affiliation: Key Laboratory of Glycoconjuates Research Ministry of Public Health and Institutes of Biomedical Sciences, Fudan University Shanghai, 200032, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200433, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Glycoconjuates Research Ministry of Public Health and Institutes of Biomedical Sciences, Fudan University Shanghai, 200032, China ; Department of Chemistry, Fudan University, Shanghai 200433, China.
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.affiliation: Key Laboratory of Glycoconjuates Research Ministry of Public Health and Institutes of Biomedical Sciences, Fudan University Shanghai, 200032, China ; Department of Chemistry, Fudan University, Shanghai 200433, China.
authors.forename: Haojie
authors.initials: H
authors.lastname: Lu
keywords: Disease proteomics; Glycosylation; Mass spectrometry