title: Purification and characterization of parvalbumin isotypes from grass carp (Ctenopharyngodon idella).
abstract: The prevalence of fish allergy is rapidly increasing because of a growing fish consumption driven mainly by a positive image of the fish and health relationship. The purpose of this study was to characterize parvalbumin isotypes from grass carp (Ctenopharyngodon idella), one of the most frequently consumed freshwater fish in China. Three parvalbumin isotypes were purified using consecutive gel filtration and reverse-phase chromatography and denoted as PVI, PVII, and PVIII. The molecular weights of the isotypes were determined to be 11.968, 11.430, and 11.512 kDa, respectively. PVI showed 74% matched amino acids sequence with PV isotype 4a from Danio rerio, while PVII and PVIII showed 46% matched amino acids sequence with PV isotypes from Hypophthalmichthys molitrix. PVII is the dominant allergen, but it was liable to gastrointestinal enzymes as PVIII; however, PVI was resistant to pepsin digestion. A further study is to characterize the epitopes of PVII, the dominant allergen.
authors.affiliation: Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University , Beijing 100083, People's Republic of China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: You
authors.forename: Yongkang
authors.initials: Y
authors.lastname: Luo
authors.forename: Jianping
authors.initials: J
authors.lastname: Wu
mesh_terms: D000485:Allergens / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D002347:Carps; D002681:China; D004044:Dietary Proteins / Q000009:adverse effects / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004063:Digestion; D029941:Fish Proteins / Q000009:adverse effects / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005512:Food Hypersensitivity / Q000209:etiology / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010320:Parvalbumins / Q000009:adverse effects / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D020033:Protein Isoforms / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011817:Rabbits; D017747:Seafood / Q000032:analysis* / Q000191:economics; D017386:Sequence Homology, Amino Acid
chemical_list: D000485:Allergens; D004044:Dietary Proteins; D029941:Fish Proteins; D010320:Parvalbumins; D020033:Protein Isoforms
keywords: IgE binding; IgG binding; characterization; parvalbumin isotypes; purification