title: The efficacy of mesenchymal stem cell transplantation in caustic esophagus injury: an experimental study.
abstract: Introduction. Ingestion of corrosive substances may lead to stricture formation in esophagus as a late complication. Full thickness injury seems to exterminate tissue stem cells of esophagus. Mesenchymal stem cells (MSCs) can differentiate into specific cell lineages and have the capacity of homing in sites of injury. Aim and Methods. We aimed to investigate the efficacy of MSC transplantation, on prevention of esophageal damage and stricture formation after caustic esophagus injury in rats. 54 rats were allocated into four groups; 4 rats were sacrificed for MSC production. Group 1, untreated controls (n: 10). Group 2, membrane labeled MSCs-treated rats (n: 20). Group 3, biodistribution of fluorodeoxyglucose labeled MSCs via positron emission tomography (PET) imaging (n: 10). Group 4, sham operated (n: 10). Standard caustic esophageal burns were created and MSCs were transplanted 24 hours after. All rats were sacrificed at the 21st days. Results. PET scan images revealed the homing behavior of MSCs to the injury site. The histopathology damage score was not significantly different from controls. However, we demonstrated Dil labeled epithelial and muscle cells which were originating from transplanted MSCs. Conclusion. MSC transplantation after caustic esophageal injury may be a helpful treatment modality; however, probably repeated infusions are needed.
authors.affiliation: Department of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kantarcioglu
authors.affiliation: Department of Pediatric Surgery, Gulhane Military Medical Academy, Etlik, 06108 Ankara, Turkey.
authors.forename: Bahadir
authors.initials: B
authors.lastname: Caliskan
authors.affiliation: Department of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Demirci
authors.affiliation: Department of Nuclear Medicine, Gulhane Military Medical Academy, Etlik, 06108 Ankara, Turkey.
authors.forename: Ozgur
authors.initials: O
authors.lastname: Karacalioglu
authors.affiliation: Department of Gastroenterology, Hitit University, 19030 Corum, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kekilli
authors.affiliation: Department of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, Turkey.
authors.forename: Zulfikar
authors.initials: Z
authors.lastname: Polat
authors.affiliation: Department of Pathology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, Turkey.
authors.forename: Armagan
authors.initials: A
authors.lastname: Gunal
authors.affiliation: Department of General Surgery, Ankara Diskapi Training and Research Hospital, 06110 Ankara, Turkey.
authors.forename: Melih
authors.initials: M
authors.lastname: Akinci
authors.affiliation: Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Baskent University, 06100 Ankara, Turkey.
authors.forename: Cagri
authors.initials: C
authors.lastname: Uysal
authors.affiliation: Department of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, Turkey.
authors.forename: Sami
authors.initials: S
authors.lastname: Eksert
authors.affiliation: Department of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Gurel
authors.affiliation: Department of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, Turkey.
authors.forename: Gurkan
authors.initials: G
authors.lastname: Celebi
authors.affiliation: Department of Hematology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, Turkey.
authors.forename: Ferit
authors.initials: F
authors.lastname: Avcu
authors.affiliation: Department of Hematology, Bayindir Hospital, 06250 Ankara, Turkey.
authors.forename: Ali Ugur
authors.initials: AU
authors.lastname: Ural
authors.affiliation: Department of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, Turkey.
authors.forename: Sait
authors.initials: S
authors.lastname: Bagci