title: Humoral autoimmune responses to insulin-like growth factor II mRNA-binding proteins IMP1 and p62/IMP2 in ovarian cancer.
abstract: Ovarian cancer is one of the leading causes of cancer-related deaths among women. There is an urgent need of better approaches for the identification of appropriate biomarkers in the early detection of ovarian cancer. The aim of this study was to elucidate the significance of autoantibodies against insulin-like growth factor II mRNA-binding proteins (IMPs) in patients with ovarian cancer. In this study, autoantibody responses to two members (IMP1 and p62/IMP2) of IMPs were evaluated by enzyme-linked immunosorbent assay (ELISA), western blotting, and indirect immunofluorescence assay in sera from patients with ovarian cancer and normal human individuals. The results have demonstrated that both IMP1 and p62/IMP2 can induce relatively higher frequency of autoantibody responses in patients with ovarian cancer (26.5% and 29.4%) compared to normal individuals (P<0.01). Our preliminary data suggest that IMP1 and p62/IMP2 can stimulate autoimmune responses in ovarian cancer, and anti-IMP1 and anti-p62/IMP2 autoantibodies could be used as potential biomarkers in immunodiagnosis of ovarian cancer.
authors.affiliation: Center for Tumor Biotherapy, The First Affiliated Hospital & College of Public Health, Zhengzhou University, Zhengzhou, Henan 450052, China ; Department of Biological Sciences & Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA.
authors.forename: Xinxin
authors.identifier: 0000-0001-9079-2010
authors.initials: X
authors.lastname: Liu
authors.affiliation: Center for Tumor Biotherapy, The First Affiliated Hospital & College of Public Health, Zhengzhou University, Zhengzhou, Henan 450052, China ; Department of Biological Sciences & Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Ye
authors.affiliation: Center for Tumor Biotherapy, The First Affiliated Hospital & College of Public Health, Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Liuxia
authors.initials: L
authors.lastname: Li
authors.affiliation: Center for Tumor Biotherapy, The First Affiliated Hospital & College of Public Health, Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Li
authors.affiliation: Center for Tumor Biotherapy, The First Affiliated Hospital & College of Public Health, Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Yi
authors.identifier: 0000-0002-5050-3541
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Tumor Biotherapy, The First Affiliated Hospital & College of Public Health, Zhengzhou University, Zhengzhou, Henan 450052, China ; Department of Biological Sciences & Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D001323:Autoantibodies / Q000097:blood / Q000235:genetics / Q000276:immunology*; D015551:Autoimmunity; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics / Q000276:immunology*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology*; D006801:Humans; D056724:Immunity, Humoral; D010051:Ovarian Neoplasms / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016601:RNA-Binding Proteins / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction
chemical_list: D001323:Autoantibodies; D014408:Biomarkers, Tumor; C406144:IGF2BP1 protein, human; C118585:P62 protein, human; D016601:RNA-Binding Proteins