title: Improvement in in vitro fertilization rate, decrease in reactive oxygen species and spermatozoa death incidence in rams by dietary fish oil.
abstract: Our aim was to evaluate the effects of fish oil feeding on sperm classical parameters, level of reactive oxygen spices (ROS), spermatozoa death incidence and in vitro fertilization (IVF) rate in rams. We randomly assigned nine rams, into two experimental groups (isoenergetic and isonitrogenous rations with constant level of vitamin E supplement): control (CTR; n = 5) and fish oil (FO; n = 4, 35 g/day/ram). Diets were fed for 70 days during the physiological breeding season. After a 21-day dietary adaptation period, semen was collected weekly from each ram by an artificial vagina. Sperm classical parameters were determined by the computer-assisted sperm analyzer system (CASA), and it was prepared for IVF process by swim-up technique. These evaluations were performed during the first and last weeks of sampling. Intracellular ROS level and spermatozoa death incidence were detected by flow cytometry on a weekly basis after adaptation. Data were analysed with SPSS 15. The volume, concentration (3.6 and 2.7 × 10(9) /ml) and sperm progressive motility (60 and 48%) were significantly improved in the FO group compared with the CTR (p < 0.05). A comparison of two-cell stage embryos following IVF in the two groups showed a significantly higher fertilization rate in the FO group (56%) compared with the CTR (49%). Superoxide anion (O2 (-) ) rate was significantly lower (p < 0.05) at the third week of sampling in the FO. Although the H2 O2 rate was numerically lower in the FO group compared with the CTR, this difference was not significant. In addition, apoptosis showed a significant difference in the third week of sampling (15 and 30% for FO and CTR, respectively; p < 0.05). Overall, adding fish oil to the ram diet not only improved sperm quality and IVF results, it also could reduce oxygen-free radicals and the incidence of spermatozoa death.
authors.affiliation: Department of Animal Science, Saveh Branch, Islamic Azad University, Saveh, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Matini Behzad
authors.affiliation: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: B
authors.initials: B
authors.lastname: Ebrahimi
authors.affiliation: Department of Animal Science, Saveh Branch, Islamic Azad University, Saveh, Iran.
authors.forename: A R
authors.initials: AR
authors.lastname: Alizadeh
authors.affiliation: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: V
authors.initials: V
authors.lastname: Esmaeili
authors.affiliation: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Dalman
authors.affiliation: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: L
authors.initials: L
authors.lastname: Rashki
authors.affiliation: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: A H
authors.initials: AH
authors.lastname: Shahverdi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001947:Breeding; D004032:Diet / Q000662:veterinary; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D005260:Female; D005307:Fertilization in Vitro / Q000706:statistics & numerical data / Q000662:veterinary*; D005395:Fish Oils / Q000008:administration & dosage*; D006861:Hydrogen Peroxide / Q000032:analysis; D008297:Male; D017382:Reactive Oxygen Species / Q000032:analysis*; D012621:Seasons; D055101:Semen Analysis / Q000379:methods / Q000662:veterinary; D012756:Sheep / Q000502:physiology*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry / Q000502:physiology*; D013481:Superoxides / Q000032:analysis
chemical_list: D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D017382:Reactive Oxygen Species; D013481:Superoxides; D006861:Hydrogen Peroxide