title: Lipid oxidation induces structural changes in biomimetic membranes.
abstract: Oxidation can intimately influence and structurally compromise the levels of biological self-assembly embodied by intracellular and plasma membranes. Lipid peroxidation, a natural metabolic outcome of life with oxygen under light, is also a salient oxidation reaction in photomedicine treatments. However, the effect of peroxidation on the fate of lipid membranes remains elusive. Here we use a new photosensitizer that anchors and disperses in the membrane to achieve spatial control of the oxidizing species. We find, surprisingly, that the integrity of unsaturated unilamellar vesicles is preserved even for fully oxidized membranes. Membrane survival allows for the quantification of the transformations of the peroxidized bilayers, providing key physical and chemical information to understand the effect of lipid oxidation on protein insertion and on other mechanisms of cell function. We anticipate that spatially controlled oxidation will emerge as a new powerful strategy for tuning and evaluating lipid membranes in biomimetic media under oxidative stress.
authors.affiliation: Present address: FOM Institute AMOLF, Science Park 104, 1098 XG Amsterdam, The Netherlands.
authors.forename: Georges
authors.initials: G
authors.lastname: Weber
authors.forename: Thierry
authors.initials: T
authors.lastname: Charitat
authors.forename: Maurício S
authors.initials: MS
authors.lastname: Baptista
authors.forename: Adjaci F
authors.initials: AF
authors.lastname: Uchoa
authors.forename: Christiane
authors.initials: C
authors.lastname: Pavani
authors.forename: Helena C
authors.initials: HC
authors.lastname: Junqueira
authors.forename: Yachong
authors.initials: Y
authors.lastname: Guo
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Baulin
authors.forename: Rosangela
authors.initials: R
authors.lastname: Itri
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Marques
authors.forename: André P
authors.initials: AP
authors.lastname: Schroder
mesh_terms: D065638:Absorption, Radiation; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry*; D015227:Lipid Peroxidation; D017319:Photosensitizing Agents / Q000494:pharmacology* / Q000528:radiation effects; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry*; D014466:Ultraviolet Rays; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D007211:Indoles; D017319:Photosensitizing Agents; D011166:Porphyrins; D053835:Unilamellar Liposomes; C000594636:chlorin-12