title: Degradation of recalcitrant aliphatic and aromatic hydrocarbons by a dioxin-degrader Rhodococcus sp. strain p52.
abstract: This study investigates the ability of Rhodococcus sp. strain p52, a dioxin degrader, to biodegrade petroleum hydrocarbons. Strain p52 can use linear alkanes (tetradecane, tetracosane, and dotriacontane), branched alkane (pristane), and aromatic hydrocarbons (naphthalene and phenanthrene) as sole carbon and energy sources. Specifically, the strain removes 85.7 % of tetradecane within 48 h at a degradation rate of 3.8 mg h(-1) g(-1) dry cells, and 79.4 % of tetracosane, 66.4 % of dotriacontane, and 63.9 % of pristane within 9-11 days at degradation rates of 20.5, 14.7, and 20.3 mg day(-1) g(-1) dry cells, respectively. Moreover, strain p52 consumes 100 % naphthalene and 55.3 % phenanthrene within 9-11 days at respective degradation rates of 16 and 12.9 mg day(-1) g(-1) dry cells. Metabolites of the petroleum hydrocarbons by strain p52 were analyzed. Genes encoding alkane-hydroxylating enzymes, including cytochrome P450 (CYP450) enzyme (CYP185) and two alkane-1-monooxygenases, were amplified by polymerase chain reaction. The transcriptional activities of these genes in the presence of petroleum hydrocarbons were detected by reverse transcription-polymerase chain reaction. The results revealed potential of strain p52 to degrade petroleum hydrocarbons.
authors.affiliation: Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, 27 ShandaNanlu, Jinan, 250100, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Yang
authors.forename: Rui-Bao
authors.initials: RB
authors.lastname: Jia
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000473:Alkanes / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism*; D009281:Naphthalenes / Q000378:metabolism*; D010578:Petroleum / Q000378:metabolism; D010616:Phenanthrenes / Q000378:metabolism*; D000072317:Polychlorinated Dibenzodioxins / Q000378:metabolism; D012240:Rhodococcus / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000473:Alkanes; D001426:Bacterial Proteins; D004785:Environmental Pollutants; D009281:Naphthalenes; D010578:Petroleum; D010616:Phenanthrenes; D000072317:Polychlorinated Dibenzodioxins; C024713:n-tetradecane; C031721:naphthalene; C031181:phenanthrene; D003577:Cytochrome P-450 Enzyme System; C514857:tetracosane