title: ER stress signaling requires RHD3, a functionally conserved ER-shaping GTPase.
abstract: Whether structure and function are correlated features of organelles is a fundamental question in cell biology. Here, we have assessed the ability of Arabidopsis mutants with a defective endoplasmic reticulum (ER) structure to invoke the unfolded protein response (UPR), an essential ER signaling pathway. Through molecular and genetic approaches, we show that loss of the ER-shaping GTPase Root Hair Defective 3 (RHD3) specifically disrupts the UPR by interfering with the mRNA splicing function of the master regulator IRE1. These findings establish a new role for RHD3 in the ER and support specificity of the effects of ER-shaping mutations on ER function.
authors.affiliation: MSU-DOE Plant Research Lab, Michigan State University, East Lansing, MI 48824, USA Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Ya-Shiuan
authors.initials: YS
authors.lastname: Lai
authors.affiliation: MSU-DOE Plant Research Lab, Michigan State University, East Lansing, MI 48824, USA Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Stefano
authors.affiliation: MSU-DOE Plant Research Lab, Michigan State University, East Lansing, MI 48824, USA Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA fb@msu.edu.
authors.forename: Federica
authors.initials: F
authors.lastname: Brandizzi
mesh_terms: D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress / Q000502:physiology*; D020558:GTP Phosphohydrolases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D013329:Structure-Activity Relationship; D014415:Tunicamycin / Q000378:metabolism; D056811:Unfolded Protein Response / Q000187:drug effects / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D014415:Tunicamycin; D011494:Protein Kinases; C446527:Ire1-1 protein, Arabidopsis; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C464507:RHD3 protein, Arabidopsis
keywords: Arabidopsis; IRE1; RHD3; Unfolded protein response