title: Post-mortem β-hydroxybutyrate determination in synovial fluid.
abstract: β-hydroxybutyrate concentrations were determined in blood and synovial fluid in a series of medico-legal cases including hypothermia fatalities, individuals found dead in a cold environment and non-hypothermia cases with various, non-traumatic causes of death. Hypothermia was considered to be the cause of death according to circumstantial elements indicating exposure to cold, autopsy findings, biochemical investigation results and exclusion of other causes of death. The intention of this study was to characterize β-hydroxybutyrate distribution in synovial fluid and assess its usefulness for the postmortem diagnosis of antemortem abnormalities in blood β-hydroxybutyrate levels. Unenhanced CT scans, autopsies, histology, neuropathology, toxicology, and biochemistry were systematically performed. Within the limited number of subjects included in the study, the results indicate that abnormalities in antemortem β-hydroxybutyrate blood levels, as may be observed in hypothermia fatalities, are reflected in postmortem synovial fluid values. These preliminary findings notwithstanding, synovial fluid analysis to determine β-hydroxybutyrate is unlikely to be generally applied due to the more invasive collection technique it requires and could be limited to special cases in which biological fluids systematically collected upon autopsy are unavailable.
authors.affiliation: University Center of Legal Medicine, Lausanne University Hospital, Rue du Bugnon 21, 1011 Lausanne, Switzerland. Electronic address: cristian.palmiere@chuv.ch.
authors.forename: Cristian
authors.initials: C
authors.lastname: Palmiere
authors.affiliation: Laboratory of Clinical Chemistry, Lausanne University Hospital, 1011 Lausanne, Switzerland.
authors.forename: Dominique
authors.initials: D
authors.lastname: Werner
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000032:analysis*; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007035:Hypothermia / Q000097:blood / Q000401:mortality; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013582:Synovial Fluid / Q000737:chemistry*; D055815:Young Adult
chemical_list: D020155:3-Hydroxybutyric Acid