title: Identification of neurotensin and LANT-6 and localization of mRNA encoding their precursor in the chicken brain.
abstract: Neurotensin (NT) and neurotensin-related peptide (Lys(8), Asn(9), NT(8-13): LANT-6) have previously been purified from chicken intestine. However, the presence of these peptides and the localization of their precursor mRNA in the brain were not well understood. In the present study, through a comprehensive analysis of bioactive substances, NT and LANT-6 were identified in the chicken brain using tandem mass spectrometry combined with a bioassay of the colon contraction. The effect of NT and LANT-6 on the colon contraction was assessed, and NT was found to be 10 times more potent than LANT-6. Furthermore, the sites of NT/LANT-6 precursor mRNA expression in the brain were investigated using quantitative RT-PCR. The result showed that the mRNA was expressed most in the telencephalon, followed by the diencephalon. In situ hybridization analysis revealed that cells containing NT/LANT-6 precursor mRNA were widely distributed throughout the brain except for the cerebellum. Additionally, these were highly concentrated in the frontal telencephalon, including the nidopallium, hyperpallium, and hippocampus. Collectively, these results indicate that NT and LANT-6 are produced in the chicken brain, and they may participate in multiple functions.
authors.affiliation: Section of Behavioral Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739-8521, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Masuda
authors.forename: Eiko
authors.initials: E
authors.lastname: Iwakoshi-Ukena
authors.forename: Yuki
authors.initials: Y
authors.lastname: Bessho
authors.forename: Shusuke
authors.initials: S
authors.lastname: Taniuchi
authors.forename: Sho
authors.initials: S
authors.lastname: Maejima
authors.forename: Kenshiro
authors.initials: K
authors.lastname: Shikano
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Kondo
authors.forename: Megumi
authors.initials: M
authors.lastname: Furumitsu
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ukena
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D009496:Neurotensin / Q000235:genetics / Q000378:metabolism*; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: D009842:Oligopeptides; D012333:RNA, Messenger; D009496:Neurotensin; C037243:neurotensin-related hexapeptide
keywords: LANT-6; brain; chicken; colon contraction; in situ hybridization; neurotensin