title: Oxidative stress response in dye degrading bacterium Lysinibacillus sp. RGS exposed to Reactive Orange 16, degradation of RO16 and evaluation of toxicity.
abstract: Lysinibacillus sp. RGS degrades sulfonated azo dye Reactive Orange 16 (RO16) efficiently. Superoxide dismutase and catalase activity were tested to study the response of Lysinibacillus sp. RGS to the oxidative stress generated by RO16. The results demonstrated that oxidative stress enzymes not only protect the cell from oxidative stress but also has a probable role in decolorization along with an involvement of oxidoreductive enzymes. Formation of three different metabolites after degradation of RO16 has been confirmed by GC-MS analysis. FTIR analysis verified the degradation of functional groups of RO16, and HPTLC confirmed the removal of auxochrome group from the RO16 after degradation. Toxicity studies confirmed the genotoxic, cytotoxic, and phytotoxic nature of RO16 and the formation of less toxic products after the treatment of Lysinibacillus sp. RGS. Therefore, Lysinibacillus sp. RGS has a better perspective of bioremediation for textile wastewater treatment.
authors.affiliation: Department of Biotechnology, Shivaji University, Kolhapur, 416004, India.
authors.forename: Priyanka A
authors.initials: PA
authors.lastname: Bedekar
authors.forename: Rijuta G
authors.initials: RG
authors.lastname: Saratale
authors.forename: Ganesh D
authors.initials: GD
authors.lastname: Saratale
authors.forename: Sanjay P
authors.initials: SP
authors.lastname: Govindwar
mesh_terms: D001391:Azo Compounds / Q000378:metabolism / Q000494:pharmacology*; D001406:Bacillaceae / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001673:Biodegradation, Environmental; D002374:Catalase / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism / Q000494:pharmacology*; D004790:Enzyme Induction; D008401:Gas Chromatography-Mass Spectrometry; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D019697:Onions / Q000187:drug effects / Q000254:growth & development; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D027805:Phaseolus / Q000187:drug effects / Q000254:growth & development; D013482:Superoxide Dismutase / Q000378:metabolism; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D001391:Azo Compounds; D001426:Bacterial Proteins; D002374:Catalase; D004396:Coloring Agents; D013482:Superoxide Dismutase; D062065:Wastewater; D014874:Water Pollutants, Chemical; C483723:reactive orange 16