title: Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender.
abstract: The aim of this study was to evaluate the effects of ergothioneine and cysteamine as antioxidant supplements in a soybean lecithin extender for freezing ram semen. Twenty-four ejaculates were collected from four rams and diluted with extenders (1.5% soybean lecithin, 7% glycerol) containing no supplements (control) and cysteamine or ergothioneine (2, 4, 6 or 8mM). Motility by CASA, viability, plasma membrane functionality (HOS test), total abnormality, lipid peroxidation, glutathione peroxidase (GPx) activity and capacitation status (CTC staining) were assessed after thawing. Using 6mM of either antioxidant improved total motility. Cysteamine at 6mM and ergothioneine at 4 and 6mM improved viability and reduced lipid peroxidation (malondialdehyde concentration). Both antioxidants improved membrane functionality significantly, except at 8mM. Progressive motility, kinematic parameters, GPx activity, capacitation status and sperm abnormalities were not influenced by the antioxidant supplements. In conclusion, cysteamine at 6mM and ergothioneine at 4 or 6mM seem to improve the post-thawing quality of ram semen cryopreserved in a soybean lecithin extender.
authors.affiliation: Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran. Electronic address: abozar.najafi@tabrizu.ac.ir.
authors.forename: Abozar
authors.initials: A
authors.lastname: Najafi
authors.affiliation: Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran. Electronic address: hsz6955@yahoo.com.
authors.forename: Hossein Daghigh
authors.initials: HD
authors.lastname: Kia
authors.affiliation: Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Mohammadi
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Mir Hossein
authors.initials: MH
authors.lastname: Najafi
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Zaynab
authors.initials: Z
authors.lastname: Zanganeh
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran; Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sharafi
authors.affiliation: INDEGSAL and Molecular Biology, University of León, León, Spain.
authors.forename: Felipe
authors.initials: F
authors.lastname: Martinez-Pastor
authors.affiliation: Department of Animal Science, College of Agriculture, University of Kurdistan, Iran.
authors.forename: Hamideh
authors.initials: H
authors.lastname: Adeldust
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003543:Cysteamine / Q000494:pharmacology*; D004880:Ergothioneine / Q000494:pharmacology*; D005979:Glutathione Peroxidase; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D037121:Plant Lectins / Q000494:pharmacology*; D012661:Semen / Q000187:drug effects; D055101:Semen Analysis; D012662:Semen Preservation; D012756:Sheep; D030262:Soybean Proteins / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D010835:Phytohemagglutinins
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D003543:Cysteamine; D004880:Ergothioneine; D005979:Glutathione Peroxidase; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins
keywords: Antioxidant; Cryopreservation; Cysteamine; Ergothioneine; Ram semen