title: Factors associated with Culicoides Obsoletus complex spp.-specific IgE reactivity in Icelandic horses and Shetland ponies.
abstract: Insect bite hypersensitivity (IBH) is a common allergic skin disease in horses, caused by biting insects of the Culicoides spp. In The Netherlands, Culicoides spp. of the Obsoletus complex are the most important midges involved in IBH. The aim of the present study was to identify and quantify associations between several endogenous (host) and exogenous (environmental) factors and immunoglobulin E (IgE) reactivity against Obsoletus complex-derived whole body extract or seven recombinant allergens, measured by ELISA. Data from 143 Icelandic horses and 177 Shetland ponies were analysed using multivariable models. In addition, the relationship between IgE reactivity and severity of clinical signs in IBH-affected horses was examined. Positive correlations were found between Obsoletus complex-specific IgE and severity of clinical signs. Disease status (IBH affected or control), breed and the interaction between IBH status and breed were significantly associated with IgE reactivity against several Obsoletus complex allergens. Significantly greater IgE reactivity was seen in IBH-affected horses compared to controls. The differences in IgE values between cases and controls were most pronounced in Icelandic horses. Shetland pony controls had significantly greater IgE reactivity compared to Icelandic horse controls, while differences in IgE values comparing Shetland pony cases and Icelandic horse cases were not significant. Severity of clinical signs and IgE reactivity in IBH-affected horses against several Obsoletus complex allergens appeared to be related. Consideration of the factors associated with Obsoletus complex-specific IgE in horses might further improve interpretation and accuracy of IgE ELISA test results within these breeds, although further research is required.
authors.affiliation: Animal Breeding and Genomics Centre, Wageningen University, PO Box 338, 6700 AH, Wageningen, The Netherlands. Electronic address: Anouk3.Schurink@wur.nl.
authors.forename: Anouk
authors.initials: A
authors.lastname: Schurink
authors.affiliation: Cell Biology and Immunology Group, Wageningen University, PO Box 338, 6700 AH, Wageningen, The Netherlands.
authors.forename: Nathalie M A
authors.initials: NM
authors.lastname: van der Meide
authors.affiliation: Cell Biology and Immunology Group, Wageningen University, PO Box 338, 6700 AH, Wageningen, The Netherlands.
authors.forename: Huub F J
authors.initials: HF
authors.lastname: Savelkoul
authors.affiliation: Animal Breeding and Genomics Centre, Wageningen University, PO Box 338, 6700 AH, Wageningen, The Netherlands.
authors.forename: Bart J
authors.initials: BJ
authors.lastname: Ducro
authors.affiliation: Cell Biology and Immunology Group, Wageningen University, PO Box 338, 6700 AH, Wageningen, The Netherlands.
authors.forename: Edwin
authors.initials: E
authors.lastname: Tijhaar
mesh_terms: D000485:Allergens / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002519:Ceratopogonidae / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000235:genetics / Q000276:immunology*; D006736:Horses; D006967:Hypersensitivity / Q000235:genetics / Q000662:veterinary*; D007073:Immunoglobulin E / Q000235:genetics* / Q000378:metabolism; D007299:Insect Bites and Stings / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009426:Netherlands; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D000485:Allergens; D019476:Insect Proteins; D007073:Immunoglobulin E
keywords: Culicoides spp.; Equine; Immunoglobulin E; Insect bite hypersensitivity; Obsoletus complex