title: [Quantitative PCR (qPCR) and the guide to good practices MIQE: adapting and relevance in the clinical biology context].
abstract: The qPCR has been introduced in clinical and biomedical research for over 10 years from now. Its use in trials and diagnostics is continuously increasing. Due to this heavy use, the question of relyability and relevance of qPCR results has to be asked. This review proposes a documented and evidence based answer to this question, thanks to the MIQE (minimum information for publication of quantitative real-time PCR experiments) guideline. The whole analysis process is addressed, from nucleic acids extraction to data management. Simple answers are given, taking into account the technical constraints from clinical research in order to allow a realistic application of this guideline.
authors.affiliation: Département SNES, Institut Polytechnique LaSalle Beauvais, Unité de recherche EGEAL UP-2012.10.101, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Dooms
authors.affiliation: Département SNES, Institut Polytechnique LaSalle Beauvais, Unité de recherche EGEAL UP-2012.10.101, France.
authors.forename: Abalo
authors.initials: A
authors.lastname: Chango
authors.affiliation: Special Infectious disease unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Afif
authors.initials: A
authors.lastname: Abdel-Nour
mesh_terms: D019983:Guideline Adherence; D006801:Humans; D017410:Practice Guidelines as Topic / Q000592:standards*; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards* / Q000706:statistics & numerical data; D048348:Reverse Transcription / Q000235:genetics; D009626:Terminology as Topic
keywords: MIQE; PCR; best practice; guideline; quantitative PCR