title: Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa.
abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) and their derivatives (aminosilane and gold-coated) have been widely investigated in numerous medical applications, including their potential to act as antibacterial drug carriers that may penetrate into bacteria cells and biofilm mass. Pseudomonas aeruginosa is a frequent cause of infection in hospitalized patients, and significant numbers of currently isolated clinical strains are resistant to standard antibiotic therapy. Here we describe the impact of three types of SPIONs on the growth of P. aeruginosa during long-term bacterial culture. Their size, structure, and physicochemical properties were determined using transmission electron microscopy, X-ray diffraction analysis, and Fourier transform infrared spectroscopy. We observed significant inhibition of P. aeruginosa growth in bacterial cultures continued over 96 hours in the presence of gold-functionalized nanoparticles (Fe₃O₄@Au). At the 48-hour time point, growth of P. aeruginosa, as assessed by the number of colonies grown from treated samples, showed the highest inhibition (decreased by 40%). These data provide strong evidence that Fe₃O₄@Au can dramatically reduce growth of P. aeruginosa and provide a platform for further study of the antibacterial activity of this nanomaterial.
authors.affiliation: Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, Bialystok, Poland ; Department of Experimental Pharmacology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Niemirowicz
authors.affiliation: Department of Microbiology, University of Bialystok, Bialystok, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Swiecicka
authors.affiliation: Institute of Chemistry, University of Bialystok, Bialystok, Poland.
authors.forename: Agnieszka Z
authors.initials: AZ
authors.lastname: Wilczewska
authors.affiliation: Institute of Chemistry, University of Bialystok, Bialystok, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Misztalewska
authors.affiliation: Institute of Chemistry, University of Bialystok, Bialystok, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Kalska-Szostko
authors.affiliation: Department of Experimental Pharmacology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Bienias
authors.affiliation: Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, Bialystok, Poland ; Faculty of Health Sciences, Jan Kochanowski University, Kielce, Poland ; Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Bucki
authors.affiliation: Department of Experimental Pharmacology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Car
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D006046:Gold / Q000008:administration & dosage* / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry; D010316:Particle Size; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D058185:Magnetite Nanoparticles; D006046:Gold
keywords: Pseudomonas aeruginosa; antibacterial activity; gold-coated nanoparticles; iron oxides; superparamagnetic nanoparticles