title: Virulence factors associated with pediatric shigellosis in Brazilian Amazon.
abstract: Shigellosis is a global human health problem and the incidence is highest among children. In the present work, main Shigella virulence genes was examined by PCR and compared to symptoms of pediatric shigellosis. Thirty Shigella isolates were identified from an etiologic study at which 1,339 children ranging 0-10 years old were enrolled. S. flexneri was the most frequent species reaching 60.0% of isolates, 22.2% were S. sonnei, and 6.6% were both S. dysenteriae and S. boydii. All Shigella infected children had diarrhea, but not all were accompanied by others symptoms of bacillary dysentery. Among major virulence genes, the PCR typing revealed ipaBCD was present in all isolates, followed by IpaH7.8, set-1A, set-1B, sen/ospD3, virF, and invE. The pathogenic potential of the ShET-1B subunit was observed in relation to dehydration (P < 0.001) and ShET-2 related to the intestinal injury (P = 0.033) evidenced by the presence of bloody diarrhea. Our results show associations among symptoms of shigellosis and virulence genes of clinical isolates of Shigella spp.
authors.affiliation: Instituto Leônidas e Maria Deane-Fiocruz Amazônia, Rua Terezina 476, Adrianópolis, 69.057-070 Manaus, AM, Brazil.
authors.forename: Carolinie Batista Nobre
authors.initials: CB
authors.lastname: da Cruz
authors.affiliation: Programa de Pós Graduação em Imunologia Básica e Aplicada (PPGBA-UFAM), Avenida General Rodrigo Octávio 6200, Coroado I, 69.077-000 Manaus, AM, Brazil.
authors.forename: Maria Carolina Scheffer
authors.initials: MC
authors.lastname: de Souza
authors.affiliation: Programa de Pós Graduação em Imunologia Básica e Aplicada (PPGBA-UFAM), Avenida General Rodrigo Octávio 6200, Coroado I, 69.077-000 Manaus, AM, Brazil.
authors.forename: Paula Taquita
authors.initials: PT
authors.lastname: Serra
authors.affiliation: Instituto Leônidas e Maria Deane-Fiocruz Amazônia, Rua Terezina 476, Adrianópolis, 69.057-070 Manaus, AM, Brazil.
authors.forename: Ivanildes
authors.initials: I
authors.lastname: Santos
authors.affiliation: Instituto Leônidas e Maria Deane-Fiocruz Amazônia, Rua Terezina 476, Adrianópolis, 69.057-070 Manaus, AM, Brazil.
authors.forename: Antonio
authors.initials: A
authors.lastname: Balieiro
authors.affiliation: Departamento de Ciências Básicas da Saúde, Universidade Federal de Juiz de Fora, Câmpus Governador Valadares, Rua Israel Pinheiro 2000, Bairro Universitário, 35010177 Governador Valadares, MG, Brazil.
authors.forename: Fabio Alessandro
authors.initials: FA
authors.lastname: Pieri
authors.affiliation: Instituto Leônidas e Maria Deane-Fiocruz Amazônia, Rua Terezina 476, Adrianópolis, 69.057-070 Manaus, AM, Brazil.
authors.forename: Paulo Afonso
authors.initials: PA
authors.lastname: Nogueira
authors.affiliation: Instituto Leônidas e Maria Deane-Fiocruz Amazônia, Rua Terezina 476, Adrianópolis, 69.057-070 Manaus, AM, Brazil.
authors.forename: Patrícia Puccinelli
authors.initials: PP
authors.lastname: Orlandi
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004405:Dysentery, Bacillary / Q000453:epidemiology* / Q000235:genetics*; D005260:Female; D060005:Genotyping Techniques; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012189:Retrospective Studies; D012760:Shigella / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D037521:Virulence Factors