title: A novel low-temperature-active pectin methylesterase from Penicillium chrysogenum F46 with high efficiency in fruit firming.
abstract: A pectin methylesterase gene (pe8F46) was cloned from Penicillium chrysogenum F46 and successfully expressed in Pichia pastoris. The full-length cDNA consists of 969 bp and encodes a 322-residue polypeptide with the calculated molecular weight of 34.1 kDa. Deduced PE8F46 belongs to family 8 of carbohydrate esterases and shares 54% identity with a functionally characterised counterpart from Myceliophthora thermophile. Purified recombinant PE8F46 showed the optimal activity at pH 5.0 and 40°C, and remained 52% maximum activity even at 10°C. An orthogonal experiment was employed to determine the best conditions for firming pineapple dices. After incubation with 0.75% (w/v) PE8F46 and 0.4% calcium lactate (w/v) for 20 min, the firmness of pineapple dices was improved by 47.6%, 13.7% higher than that of a commercial pectinase complex. These results suggest that PE8F46 has application potential in the food industry.
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Pan
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tu
authors.affiliation: National Animal Husbandry Extension Service, Beijing 100125, People's Republic of China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Luo
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Pengjun
authors.initials: P
authors.lastname: Shi
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China. Electronic address: mengkun@caas.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Meng
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China. Electronic address: yaobin@caas.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
mesh_terms: D000595:Amino Acid Sequence; D031230:Ananas; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005525:Food-Processing Industry / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010408:Penicillium chrysogenum / Q000201:enzymology* / Q000235:genetics; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism; D011994:Recombinant Proteins; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D011096:Polygalacturonase
keywords: Low-temperature-active; Pectin methylesterase; Penicillium chrysogenum; Pineapple firmness