title: Diversin is overexpressed in breast cancer and accelerates cell proliferation and invasion.
abstract: Diversin was recently reported to play roles in Wnt and JNK pathways. However, the expression pattern and biological roles of diversin in human breast cancer have not been reported. In the present study, we found that diversin was overexpressed in breast cancer specimens by immunohistochemistry and western blot. Significant association was observed between diversin overexpression and TNM stage (p = 0.0036), nodal metastasis (p = 0.0033), negative estrogen receptor expression (p = 0.0012) and triple-negative status (p = 0.0017). Furthermore, colony formation assay and matrigel invasion assay showed that knockdown of diversin expression in MDA-MB-231 cell line with high endogenous expression decreased cell proliferation and cell invasion. Transfection of diversin plasmid in MCF-7 cell line increased cell proliferation and invasion. Further analysis showed that diversin depletion downregulated JNK phosphorylation while its overexpression upregulated JNK phosphorylation. In conclusion, our study demonstrated that diversin was overexpressed in human breast cancers. Diversin could contribute to breast cancer cell proliferation and invasion.
authors.affiliation: Department of Surgical Oncology and Breast Surgery, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Minghao
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of pathology, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Qianze
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jin
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D048670:MAP Kinase Kinase 4 / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009367:Neoplasm Staging; D010766:Phosphorylation / Q000235:genetics; D060449:Wnt Signaling Pathway
chemical_list: C464902:ANKRD6 protein, human; D003598:Cytoskeletal Proteins; D009363:Neoplasm Proteins; D048670:MAP Kinase Kinase 4