title: Benefits of the antioxidant and anti-inflammatory activity of etoricoxib in the prevention of ovarian ischemia/reperfusion injury induced experimentally in rats.
abstract: OBJECTIVE
  This study is a biochemical investigation of the effect of etoricoxib, a selective cyclooxygenase (COX)-2 inhibitor, on ischemia/reperfusion (I/R) injury experimentally induced in rat ovaries.
  
  
  METHODS
  Experimental animals were divided into four groups: (i) ovarian ischemia/reperfusion (IRG); (ii) 30 mg/kg etoricoxib + ovarian ischemia/reperfusion (EIRG-30); (iii) 60 mg/kg etoricoxib + ovarian ischemia/reperfusion (EIRG-60); and (iv) a sham operation (SG) control group.
  
  
  RESULTS
  The results showed levels of malondialdehyde in the IRG, EIRG-30, EIRG-60 and SG group ovarian tissue of 20.2 ± 3.4, 11.2 ± 3.2, 5.5 ± 1.9 and 3.8 ± 1.5 μmol/g protein, respectively. Myeloperoxidase activity for these groups was 24.2 ± 6.7, 13 ± 2.4, 4 ± 1.8 and 3.5 ± 1.9 U/g protein, and total glutathione levels were 1.6 ± 0.8, 4.5 ± 1.9, 6.5 ± 1.9 and 7.5 ± 2.4 nmol/g protein, respectively. COX-1 activity in IRG, EIRG-30, EIRG-60 and SG group rat ovarian tissue was 5.0 ± 2.8, 12.2 ± 2.4, 16.7 ± 2.8 and 17.5 ± 4.7 U/mg protein, and COX-2 activity was 18.3 ± 2.7, 3.5 ± 1, 1.8 ± 0.7 and 0.7 ± 0.3 U/mg protein, respectively.
  
  
  CONCLUSIONS
  Etoricoxib prevented oxidative damage induced with I/R in rat ovarian tissue. This property of etoricoxib suggests that it can be clinically beneficial in the prevention of damage that may arise with reperfusion by detorsion for the protection of the ovaries against torsion.
authors.affiliation: Obstetrics and Gynecology, Sorgun State Hospital, Yozgat.
authors.forename: Omer Erkan
authors.initials: OE
authors.lastname: Yapca
authors.forename: Mehmet Ibrahim
authors.initials: MI
authors.lastname: Turan
authors.forename: Ismayil
authors.initials: I
authors.lastname: Yilmaz
authors.forename: Suleyman
authors.initials: S
authors.lastname: Salman
authors.forename: Mine
authors.initials: M
authors.lastname: Gulapoglu
authors.forename: Halis
authors.initials: H
authors.lastname: Suleyman
mesh_terms: D000818:Animals; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D000077613:Etoricoxib; D005260:Female; D005978:Glutathione / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D010053:Ovary / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology / Q000627:therapeutic use*; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D013450:Sulfones / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D008565:Membrane Proteins; D011725:Pyridines; D013450:Sulfones; D008315:Malondialdehyde; D009195:Peroxidase; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496513:Ptgs1 protein, rat; C489691:Ptgs2 protein, rat; D005978:Glutathione; D000077613:Etoricoxib
keywords: etoricoxib; ischemia; ovary; oxidants; rat