title: Highly efficient differentiation of embryonic stem cells into adipocytes by ascorbic acid.
abstract: Adipocyte differentiation and function have become the major research targets due to the increasing interest in obesity and related metabolic conditions. Although, late stages of adipogenesis have been extensively studied, the early phases remain poorly understood. Here we present that supplementing ascorbic acid (AsA) to the adipogenic differentiation cocktail enables the robust and efficient differentiation of mouse embryonic stem cells (mESCs) to mature adipocytes. Such ESC-derived adipocytes mimic the gene-expression profile of subcutaneous isolated adipocytes in vivo remarkably well, much closer than 3T3-L1 derived ones. Moreover, the differentiated cells are in a monolayer, allowing a broad range of genome-wide studies in early and late stages of adipocyte differentiation to be performed.
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen, Medical and Health Science Center, Egyetem tér 1, Debrecen H-4012, Hungary.
authors.forename: Ixchelt
authors.initials: I
authors.lastname: Cuaranta-Monroy
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen, Medical and Health Science Center, Egyetem tér 1, Debrecen H-4012, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Simandi
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen, Medical and Health Science Center, Egyetem tér 1, Debrecen H-4012, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Kolostyak
authors.affiliation: Department of Biophysics and Cell Biology, Medical and Health Science Center, University of Debrecen, Egyetem tér 1, Debrecen H-4012, Hungary.
authors.forename: Quang-Minh
authors.initials: QM
authors.lastname: Doan-Xuan
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen, Medical and Health Science Center, Egyetem tér 1, Debrecen H-4012, Hungary.
authors.forename: Szilard
authors.initials: S
authors.lastname: Poliska
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen, Medical and Health Science Center, Egyetem tér 1, Debrecen H-4012, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Horvath
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen, Medical and Health Science Center, Egyetem tér 1, Debrecen H-4012, Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Nagy
authors.affiliation: Department of Biophysics and Cell Biology, Medical and Health Science Center, University of Debrecen, Egyetem tér 1, Debrecen H-4012, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Bacso
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen, Medical and Health Science Center, Egyetem tér 1, Debrecen H-4012, Hungary; MTA-DE "Lendulet" Immunogenomics Research Group, Hungary. Electronic address: lnagy@sanfordburnham.org.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Nagy
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D003584:Cytological Techniques / Q000379:methods; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D051379:Mice
chemical_list: D001205:Ascorbic Acid