title: Taxonomic database and cut-off value for processing mcrA gene 454 pyrosequencing data by MOTHUR.
abstract: The functional mcrA gene of methanogens can generate phylogeny as congruent as the 16S rRNA gene phylogeny. For the mcrA sequences amplified by mlas/mcrA-rev primers, we created a database for taxonomical classification and propose cut-off values for OTU clustering and further analysis of α- and β-diversity with the MOTHUR software.
authors.affiliation: GFZ German Research Centre for Geosciences, Section 4.5: Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany. Electronic address: sizhong.yang@gfz-potsdam.de.
authors.forename: Sizhong
authors.initials: S
authors.lastname: Yang
authors.affiliation: GFZ German Research Centre for Geosciences, Section 4.5: Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Liebner
authors.affiliation: GFZ German Research Centre for Geosciences, Section 4.5: Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany.
authors.forename: Mashal
authors.initials: M
authors.lastname: Alawi
authors.affiliation: GFZ German Research Centre for Geosciences, Section 4.5: Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Ebenhöh
authors.affiliation: GFZ German Research Centre for Geosciences, Section 4.5: Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Wagner
mesh_terms: D019295:Computational Biology / Q000379:methods; D030561:Databases, Nucleic Acid; D019766:Genes, Archaeal; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D019638:Methanomicrobiaceae / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012016:Reference Values; D012984:Software
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Cut-off; MOTHUR; Methanogens; Taxonomic database; mcrA gene