title: Efficient strategy for maintaining and enhancing the huperzine A production of Shiraia sp. Slf14 through inducer elicitation.
abstract: Huperzine A (HupA), a naturally occurring lycopodium alkaloid, is a potent, highly specific and reversible inhibitor of acetylcholinesterase and is a potential treatment for Alzheimer's disease. However, isolating HupA from Huperziaceae plants is inefficient; thus, extracting this compound from endophytic fungi may be more controllable and sustainable. However, the large-scale production of this chemical from endophytes is limited by the innate instability of endophytic fungi. In this study, we maintained the stability and viability of the HupA-producing endophytic fungus Shiraia sp. Slf14 and enhanced the HupA titers during fermentation by adding Huperzia serrata extracts (HSE), L-lysine, and acetic acid into the culture as inducers. Adding trace amounts of HupA clearly improved the HupA production of Shiraia sp. Slf14, reaching a maximum content of approximately 40 μg g(-1). Moreover, the addition of HSE and L-lysine promoted HupA production in the flask fermentation. The aforementioned bioprocessing strategy may be potentially applied to other endophytic fungal culture systems for the efficient production of plant secondary metabolites.
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, 330022, China.
authors.forename: Riming
authors.initials: R
authors.lastname: Yan
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.forename: Huilin
authors.initials: H
authors.lastname: Yang
authors.forename: Qinggui
authors.initials: Q
authors.lastname: Zeng
authors.forename: Du
authors.initials: D
authors.lastname: Zhu
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis* / Q000302:isolation & purification; D000544:Alzheimer Disease / Q000188:drug therapy; D001203:Ascomycota / Q000187:drug effects* / Q000378:metabolism*; D002457:Cell Extracts / Q000494:pharmacology; D060026:Endophytes / Q000187:drug effects / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D032501:Huperzia / Q000737:chemistry / Q000166:cytology; D008239:Lysine / Q000378:metabolism / Q000494:pharmacology; D050296:Microbial Viability / Q000187:drug effects; D064210:Secondary Metabolism; D012717:Sesquiterpenes / Q000302:isolation & purification
chemical_list: D000470:Alkaloids; D002457:Cell Extracts; D012717:Sesquiterpenes; C050426:huperzine A; D008239:Lysine