title: CCHCR1 interacts with EDC4, suggesting its localization in P-bodies.
abstract: Coiled-coil alpha-helical rod protein 1 (CCHCR1) is suggested as a candidate biomarker for psoriasis for more than a decade but its function remains poorly understood because of the inconsistent findings in the literature. CCHCR1 protein is suggested to be localized in the cytoplasm, nucleus, mitochondria, or centrosome and to regulate various cellular functions, including steroidogenesis, proliferation, differentiation, and cytoskeleton organization. In this study, we attempted to find a consensus between these findings by identifying the interaction partners of CCHCR1 using co-immunoprecipiation with a stable cell line expressing EGFP-tagged CCHCR1. Out of more than 100 co-immunoprecipitants identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS), the enhancer of mRNA-decapping protein 4 (EDC4), which is a processing body (P-body) component, was particularly found to be the major interacting partner of CCHCR1. Confocal imaging confirmed the localization of CCHCR1 in P-bodies and its N-terminus is required for this subcellular localization, suggesting that CCHCR1 is a novel P-body component. As P-bodies are the site for mRNA metabolism, our findings provide a molecular basis for the function of CCHCR1, any disruption of which may affect the transcriptome of the cell, and causing abnormal cell functions.
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Ling
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
authors.forename: C C
authors.initials: CC
authors.lastname: Wong
authors.affiliation: Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University, 1081 HV Amsterdam, The Netherlands.
authors.forename: K W
authors.initials: KW
authors.lastname: Li
authors.affiliation: School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
authors.forename: K M
authors.initials: KM
authors.lastname: Chan
authors.affiliation: Division of Genetics of Skin Carcinogenesis, A110 German Cancer Research Center, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Boukamp
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. Electronic address: ken-liu@cuhk.edu.hk.
authors.forename: W K
authors.initials: WK
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D022083:Cytoplasmic Structures / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D011565:Psoriasis / Q000378:metabolism
chemical_list: C412616:CCHCR1 protein, human; C507914:EDC4 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: CCHCR1; EDC4; LC-MS/MS; P-bodies