title: The effect of Na-selenite treatment on the oxidative stress-antioxidants balance of multiple organ failure.
abstract: OBJECTIVE
  Our study tested the hypothesis that sodium (Na)-selenite expression treatment can reduce oxidative stress and increase plasma antioxidants, whereas modulating white blood cell antigen expression in severe sepsis. Selenite is a well known cofactor of glutathione peroxidases and other antioxidant enzymes; therefore, one may expect an antioxidant effect of treatment.
  
  
  METHODS
  We randomized 40 severe septic patients into treatment and control groups. Treatment group (n = 21) received 1000-μg/2 hours Na-selenite load, followed by a 1000-μg/die medication. Oxidative stress markers, including malondialdehyde, maximal free radical production, and plasma antioxidants: free sulfhydryl groups, glutathione levels, and superoxide dismutase and catalase enzyme activity were measured.
  
  
  RESULTS
  According to our results, the treatment regime successfully restored serum selenium levels. Treatment group developed a significant malondialdehyde increase by the fifth study day, whereas reactive oxygen species production decreased significantly. Reduced glutathione and plasma sulfhydryl groups showed no significant difference. Treatment group showed deteriorated expression of CD11a and slight increase of CD49d expression on monocytes throughout our study.
  
  
  CONCLUSIONS
  Although our Na-selenite treatment regime successfully restored the selenium deficiency of severe septic patients, antioxidant and white blood cell antigen expression modulating effect of the therapy was not observed in our patient group.
authors.affiliation: University of Pécs, Department of Anaesthesia and Intensive Therapy, Pécs, Hungary. Electronic address: glwoth@gmail.com.
authors.forename: Gábor
authors.initials: G
authors.lastname: Woth
authors.affiliation: University of Pécs, Department of Anaesthesia and Intensive Therapy, Pécs, Hungary.
authors.forename: Bálint
authors.initials: B
authors.lastname: Nagy
authors.affiliation: University of Pécs, Department of Anaesthesia and Intensive Therapy, Pécs, Hungary.
authors.forename: Ákos
authors.initials: Á
authors.lastname: Mérei
authors.affiliation: University of Pécs, Medical School, Pécs, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Ernyey
authors.affiliation: University of Pécs, Medical School, Pécs, Hungary.
authors.forename: Réka
authors.initials: R
authors.lastname: Vincze
authors.affiliation: University of Pécs, Medical School, Pécs, Hungary.
authors.forename: Zita
authors.initials: Z
authors.lastname: Kaurics
authors.affiliation: University of Pécs, Department of Surgical Research and Techniques, Pécs, Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Lantos
authors.affiliation: University of Pécs, Department of Anaesthesia and Intensive Therapy, Pécs, Hungary.
authors.forename: Lajos
authors.initials: L
authors.lastname: Bogár
authors.affiliation: University of Pécs, Department of Anaesthesia and Intensive Therapy, Pécs, Hungary.
authors.forename: Diana
authors.initials: D
authors.lastname: Mühl
mesh_terms: D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D040881:CD11a Antigen / Q000097:blood; D002374:Catalase / Q000097:blood; D005260:Female; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase; D006801:Humans; D039441:Integrin alpha4 / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D009102:Multiple Organ Failure / Q000188:drug therapy; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000097:blood; D018805:Sepsis / Q000097:blood / Q000188:drug therapy*; D018038:Sodium Selenite / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000097:blood; D014131:Trace Elements / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D040881:CD11a Antigen; D017382:Reactive Oxygen Species; D014131:Trace Elements; D039441:Integrin alpha4; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D012643:Selenium; D018038:Sodium Selenite
keywords: Inflammation; Selenium; Selenoproteins; Sepsis