title: Anti-hnRNP B1 (RA33) autoantibodies are associated with the clinical phenotype in Russian patients with rheumatoid arthritis and systemic sclerosis.
abstract: Heterogeneous nuclear ribonucleoproteins (hnRNPs) are potent autoantigenic targets in systemic autoimmune rheumatic diseases (SARD). Loss of tolerance to the RA33 complex consisting of hnRNP A2 and its alternatively spliced variants B1 and B2 has been the interest of rheumatologists. A novel ELISA for the detection of anti-hnRNP B1 autoantibodies has been developed to investigate the prevalence thereof in 397 patients with SARD, including patients with rheumatoid arthritis (RA), spondyloarthropathy (SPA), juvenile chronic arthritis, systemic lupus erythematosus (SLE), systemic sclerosis (SSc), and Sjögren's syndrome (SS), in comparison to 174 controls. Anti-hnRNP B1 autoantibodies were significantly more prevalent in patients with SARD than controls (47/397, 11.8% versus 2/174, 1.1%; P<0.001). In particular, anti-hnRNP B1 were found more frequently in the disease cohorts than in the controls and were present in 24/165 (14.5%) patients with RA, 6/58 (10.3%) SPA, 11/65 (16.9%) SSc, and 4/50 (8.0%) SLE. In RA patients, anti-hnRNP B1 autoantibodies correlated significantly with C-reactive protein levels and erythrocyte sedimentation rate, while in patients with SSc it was associated with features of arterial wall stiffness and presence of hypertension. Anti-hnRNP B1 autoantibodies occur in SARD and seem to be correlated with distinct clinical characteristics in patients with RA and SSc.
authors.affiliation: Department of Rheumatology, Almazov Medical Research Centre, 197341 St. Petersburg, Russia.
authors.forename: Aleksey
authors.initials: A
authors.lastname: Maslyanskiy
authors.affiliation: Laboratory of Autoimmune Diagnostics, St. Petersburg Pavlov State Medical University, 197022 St. Petersburg, Russia.
authors.forename: Natalya
authors.initials: N
authors.lastname: Lazareva
authors.affiliation: Eichwald Department of Therapy and Rheumatology, Mechnikov Northwestern State University, 191015 St. Petersburg, Russia.
authors.forename: Polina
authors.initials: P
authors.lastname: Olinek
authors.affiliation: Faculty of Science, Brandenburg University of Technology Cottbus-Senftenberg, Großenhainer Straße 57, 01968 Senftenberg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schierack
authors.affiliation: R/D, Medipan GmbH, Dahlewitz, 15827 Berlin, Germany.
authors.forename: Christian
authors.identifier: 0000-0003-0579-202X
authors.initials: C
authors.lastname: Hentschel
authors.affiliation: R/D, Medipan GmbH, Dahlewitz, 15827 Berlin, Germany.
authors.forename: Juliane
authors.initials: J
authors.lastname: Cuccato
authors.affiliation: Institute of Liver Studies, Division of Transplantation Immunology and Mucosal Biology, King's College London School of Medicine at King's College Hospital, London SES 9RJ, UK.
authors.forename: Dimitrios P
authors.initials: DP
authors.lastname: Bogdanos
authors.affiliation: Laboratory of Autoimmune Diagnostics, St. Petersburg Pavlov State Medical University, 197022 St. Petersburg, Russia.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Lapin
authors.affiliation: Faculty of Science, Brandenburg University of Technology Cottbus-Senftenberg, Großenhainer Straße 57, 01968 Senftenberg, Germany ; R/D, Medipan GmbH, Dahlewitz, 15827 Berlin, Germany.
authors.forename: Dirk
authors.identifier: 0000-0002-6861-6827
authors.initials: D
authors.lastname: Roggenbuck
mesh_terms: D000293:Adolescent; D000328:Adult; D001171:Arthritis, Juvenile / Q000175:diagnosis / Q000276:immunology / Q000503:physiopathology; D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000276:immunology / Q000503:physiopathology*; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000097:blood*; D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000276:immunology / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000037:antagonists & inhibitors / Q000097:blood*; D006801:Humans; D006973:Hypertension / Q000503:physiopathology; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000276:immunology / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D012426:Russia; D012595:Scleroderma, Systemic / Q000175:diagnosis / Q000276:immunology / Q000503:physiopathology*; D012859:Sjogren's Syndrome / Q000175:diagnosis / Q000276:immunology / Q000503:physiopathology; D025242:Spondylarthropathies / Q000175:diagnosis / Q000276:immunology / Q000503:physiopathology; D059289:Vascular Stiffness
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; C439768:hnRNP A2; D002097:C-Reactive Protein