title: Sulfated exopolysaccharide produced by Labrenzia sp. PRIM-30, characterization and prospective applications.
abstract: Exopolysaccharides (EPS) are biopolymers produced by bacteria, having potential industrial applications. An EPS produced by a bacterium designated as PRIM-30 isolated from the deep seawater collected from offshore region of Cochin, India was studied. The isolate was identified as Labrenzia sp. based on 16S rRNA gene sequencing. Under optimum conditions for EPS production, the EPS yield was 840mgL(-1) culture medium. The average molecular weight of the EPS was 269kDa and composed of glucose, arabinose, galacturonic acid and mannose in the ratio 14.4:1.2:1:0.6. Importantly, the purified EPS contained 4.76% (w/w) sulfate groups. Viscosity of the (1% w/v) EPS was 3.92cP (shear rate 300s(-1), 25°C). Anodic peak potential (Epa) of the EPS by cyclic voltametric measurement was -0.7V. The EPS showed antioxidant activities with IC50 values of 640 and 190μgmL(-1), respectively, for the inhibition of DPPH and superoxide radicals. The EPS displayed a linear dose dependent increase in total antioxidant capacity and ferric reducing power activities. To date, only a very few marine alphaproteobacterial representatives have been reported for EPS production and this study for the first time, shows the production of a sulfated EPS by a member of the genus Labrenzia.
authors.affiliation: Yenepoya Research Centre, Yenepoya University, University Road, Deralakatte, Mangalore 575018, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: P
authors.affiliation: Yenepoya Research Centre, Yenepoya University, University Road, Deralakatte, Mangalore 575018, India.
authors.forename: Arun
authors.initials: A
authors.lastname: A B
authors.affiliation: Yenepoya Research Centre, Yenepoya University, University Road, Deralakatte, Mangalore 575018, India. Electronic address: rekhapd@hotmail.com.
authors.forename: Rekha
authors.initials: R
authors.lastname: P D
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D007194:India; D010851:Picrates / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D041902:Rhodobacteraceae / Q000145:classification / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology; D013431:Sulfates / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011135:Polysaccharides, Bacterial; D013431:Sulfates; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activity; Labrenzia; Marine Alphaproteobacteria; Sulfated-exopolysaccharide