title: Clinical relevance of IL-6 gene polymorphism in severely injured patients.
abstract: In polytrauma, injuries that may be surgically treated under regular circumstances due to a systemic inflammatory response become life-threatening. The inflammatory response involves a complex pattern of humoral and cellular responses and the expression of related factors is thought to be governed by genetic variations. This aim of this paper is to examine the influence of interleukin (IL) 6 single nucleotide polymorphism (SNP) -174C/G and -596G/A on the treatment outcome in severely injured patients. Forty-seven severely injured patients were included in this study. Patients were assigned an Injury Severity Score. Blood samples were drawn within 24 h after admission (designated day 1) and on subsequent days (24, 48, 72 hours and 7 days) of hospitalization. The IL-6 levels were determined through ELISA technique. Polymorphisms were analyzed by a method of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR). Among subjects with different outcomes, no statistically relevant difference was found with regards to the gene IL-6 SNP-174G/C polymorphism. More than a half of subjects who died had the SNP-174G/C polymorphism, while this polymorphism was represented in a slightly lower number in survivors. The incidence of subjects without polymorphism and those with heterozygous and homozygous gene IL-6 SNP-596G/A polymorphism did not present statistically significant variations between survivors and those who died. The levels of IL-6 over the observation period did not present any statistically relevant difference among subjects without the IL-6 SNP-174 or IL- 6 SNP -596 gene polymorphism and those who had either a heterozygous or a homozygous polymorphism.
authors.affiliation: School of Medicine, University of Belgrade, Dr Subotica street 8, 11000 Belgrade, Serbia.
authors.forename: Vasilije
authors.initials: V
authors.lastname: Jeremić
authors.affiliation: School of Medicine, University of Belgrade, Dr Subotica street 8, 11000 Belgrade, Serbia.
authors.forename: Tamara
authors.initials: T
authors.lastname: Alempijević
authors.affiliation: Clinic for Emergency Surgery, Clinical Centre of Serbia, Pasterova street 2, 11000 Belgrade, Serbia.
authors.forename: Srđan
authors.initials: S
authors.lastname: Mijatović
authors.affiliation: School of Medicine, University of Belgrade, Dr Subotica street 8, 11000 Belgrade, Serbia.
authors.forename: Ana
authors.initials: A
authors.lastname: Sijački
authors.affiliation: Clinic for Gastroenterology and Hepatology, Clinical Centre of Serbia, Dr Koste Todorovica Street 2, 11000 Belgrade, Serbia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Dragašević
authors.affiliation: Laboratory for Molecular Biomedicine, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Pasterova street 2, 11000 Belgrade, Serbia.
authors.forename: Sonja
authors.initials: S
authors.lastname: Pavlović
authors.affiliation: Institute for Medical Informatics, School of Dentistry, University of Belgrade, Dr Subotica street 15,11000 Belgrade, Serbia.
authors.forename: Biljana
authors.initials: B
authors.lastname: Miličić
authors.affiliation: School of Medicine, University of Belgrade, Dr Subotica street 8, 11000 Belgrade, Serbia.
authors.forename: Slobodan
authors.initials: S
authors.lastname: Krstić
mesh_terms: D000328:Adult; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006760:Hospitalization; D006801:Humans; D015601:Injury Severity Score; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009104:Multiple Trauma / Q000097:blood / Q000235:genetics* / Q000401:mortality*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015996:Survival Rate; D013997:Time Factors; D055815:Young Adult
chemical_list: D015850:Interleukin-6
keywords: IL-6; polymorphism; severe injury