title: The induction of maturation on dendritic cells by TiO2 and Fe(3)O(4)@TiO(2) nanoparticles via NF-κB signaling pathway.
abstract: Nanomaterials are increasingly used in many fields, including drug vectors and vaccine formulation. In this study, nano-TiO(2) and magnetic Fe(3)O(4)@TiO(2) were synthesized and their abilities to activate dendritic cells were investigated. The signaling pathway involved in their effects on the cellular functions was also explored. First, nano-TiO(2) and Fe(3)O(4)@TiO(2) were prepared with diameters of 82nm and 63nm, and zeta potentials of 41.5mV and 30.2mV, respectively. The magnetic property of Fe(3)O(4)@TiO(2) was detected to be 12.9emu/g. Both kinds of nanoparticles were proved to have good biocompatibility in vitro. Second, the exposure of nano-TiO2 and Fe(3)O(4)@TiO(2)caused an increased expression of TNF-α, CD86 and CD80, and besides, Fe(3)O(4)@TiO(2)showed a certain up-regulation on MHC-II. The cellular uptake of Ovalbumin on BMDCs could be strongly improved by nano-TiO2 and Fe(3)O(4)@TiO(2)as detected via flow cytometer and confocal observation. Further investigation revealed that nano-TiO(2) and Fe(3)O(4)@TiO(2)significantly increased the NF-κB expression in the nucleus, indicating that the NF-κB signaling pathway was involved in the dendritic cell maturation. Our results suggested that nano-TiO(2) and Fe(3)O(4)@TiO(2)may function as a useful vector to promote vaccine delivery in immune cells, and Fe(3)O(4)@TiO(2)provided a possibility to deliver and track vaccines via its magnetofection.
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, PR China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, PR China.
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, PR China.
authors.forename: Xiling
authors.initials: X
authors.lastname: Du
authors.affiliation: Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, PR China. Electronic address: liuhuigg@hotmail.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, PR China. Electronic address: wsl@tongji.edu.cn.
authors.forename: Shilong
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D001672:Biocompatible Materials; D002460:Cell Line; D055598:Chemical Phenomena; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000235:genetics*; D010047:Ovalbumin / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D014025:Titanium / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D001672:Biocompatible Materials; C497965:Cd86 protein, mouse; D005290:Ferric Compounds; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C009495:titanium dioxide; C000499:ferric oxide; D010047:Ovalbumin; D014025:Titanium
keywords: Dendritic cells; Fe(3)O(4)@TiO(2); Maturation; NF-κB; TiO(2)