title: MicroRNA expression profile in myocardial bridging patients.
abstract: BACKGROUND
  Myocardial bridging (MB), a common benign coronary anomaly, may bring about some unwanted complications such as angina-like chest pain. The only way of MB detection is coronary arteriography or coronary computed tomographic angiography, which is costly and invasive. This study intended to profile a panel of circulating microRNAs (miRNAs) as potential biomarkers for the diagnosis of MB.
  
  
  METHODS
  Using TaqMan Low-Density Array followed by quantitative reverse transcriptase PCR (qRT-PCR) validation, we analyzed the expression of miRNAs in serum samples from 90 MB patients and 50 non-MB controls.
  
  
  RESULTS
  The Low-Density Array data showed that 196 miRNAs were differentially expressed in MB patient sera in comparison with controls. After qRT-PCR validation and receiver operating characteristic (ROC) analysis, a list of five miRNAs (miR-29b, miR-151-3p, miR-126, miR-503-3p and miR-645) showed the ability to distinguish MB patients from controls. The area under curve (AUC) values range from 0.722-0.938.
  
  
  CONCLUSIONS
  We have demonstrated that this panel of five serum miRNAs is expected to become potential non-invasive biomarkers for detection of MB.
authors.affiliation: From Jinling Hospital, Department of Cardiology, Nanjing University, School of Medicine , Nanjing , P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhong
authors.forename: Ying-Hao
authors.initials: YH
authors.lastname: Pei
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Jiao
authors.initials: J
authors.lastname: Chen
authors.forename: Shi-Sen
authors.initials: SS
authors.lastname: Jiang
authors.forename: Jian-Bin
authors.initials: JB
authors.lastname: Gong
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D054084:Myocardial Bridging / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012372:ROC Curve
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: Eerum microRNA; biomarker; diagnosis; myocardial bridge; qRT-PCR