title: Latent phenotypes pervade gene regulatory circuits.
abstract: BACKGROUND
  Latent phenotypes are non-adaptive byproducts of adaptive phenotypes. They exist in biological systems as different as promiscuous enzymes and genome-scale metabolic reaction networks, and can give rise to evolutionary adaptations and innovations. We know little about their prevalence in the gene expression phenotypes of regulatory circuits, important sources of evolutionary innovations.
  
  
  RESULTS
  Here, we study a space of more than sixteen million three-gene model regulatory circuits, where each circuit is represented by a genotype, and has one or more functions embodied in one or more gene expression phenotypes. We find that the majority of circuits with single functions have latent expression phenotypes. Moreover, the set of circuits with a given spectrum of functions has a repertoire of latent phenotypes that is much larger than that of any one circuit. Most of this latent repertoire can be easily accessed through a series of small genetic changes that preserve a circuit's main functions. Both circuits and gene expression phenotypes that are robust to genetic change are associated with a greater number of latent phenotypes.
  
  
  CONCLUSIONS
  Our observations suggest that latent phenotypes are pervasive in regulatory circuits, and may thus be an important source of evolutionary adaptations and innovations involving gene regulation.
authors.forename: Joshua L
authors.initials: JL
authors.lastname: Payne
authors.affiliation: University of Zurich, Zurich, Switzerland. andreas.wagner@ieu.uzh.ch.
authors.forename: Andreas
authors.initials: A
authors.lastname: Wagner
mesh_terms: D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D005838:Genotype; D008957:Models, Genetic; D010641:Phenotype