title: Osteoconductive potential of barrier nanoSiO2 PLGA membranes functionalized by plasma enhanced chemical vapour deposition.
abstract: The possibility of tailoring membrane surfaces with osteoconductive potential, in particular in biodegradable devices, to create modified biomaterials that stimulate osteoblast response should make them more suitable for clinical use, hopefully enhancing bone regeneration. Bioactive inorganic materials, such as silica, have been suggested to improve the bioactivity of synthetic biopolymers. An in vitro study on HOB human osteoblasts was performed to assess biocompatibility and bioactivity of SiO2 functionalized poly(lactide-co-glycolide) (PLGA) membranes, prior to clinical use. A 15 nm SiO2 layer was deposited by plasma enhanced chemical vapour deposition (PECVD), onto a resorbable PLGA membrane. Samples were characterized by X-ray photoelectron spectroscopy, atomic force microscopy, scanning electron microscopy, and infrared spectroscopy (FT-IR). HOB cells were seeded on sterilized test surfaces where cell morphology, spreading, actin cytoskeletal organization, and focal adhesion expression were assessed. As proved by the FT-IR analysis of samples, the deposition by PECVD of the SiO2 onto the PLGA membrane did not alter the composition and other characteristics of the organic membrane. A temporal and spatial reorganization of cytoskeleton and focal adhesions and morphological changes in response to SiO2 nanolayer were identified in our model. The novedous SiO2 deposition method is compatible with the standard sterilization protocols and reveals as a valuable tool to increase bioactivity of resorbable PLGA membranes.
authors.affiliation: Nanotechnology on Surfaces Laboratory, Instituto de Ciencia de Materiales de Sevilla (CSIC), Universidad de Sevilla, Avenida Américo Vespucio 49, 41092 Seville, Spain.
authors.forename: Antonia
authors.initials: A
authors.lastname: Terriza
authors.affiliation: Facultad de Medicina, Servicios Centrales de Investigación en Ciencias de la Salud, University of Cadiz, Dr. Marañon 3, 11002 Cadiz, Spain.
authors.forename: Jose I
authors.initials: JI
authors.lastname: Vilches-Pérez
authors.affiliation: Facultad de Medicina, Servicios Centrales de Investigación en Ciencias de la Salud, University of Cadiz, Dr. Marañon 3, 11002 Cadiz, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: de la Orden
authors.affiliation: Nanotechnology on Surfaces Laboratory, Instituto de Ciencia de Materiales de Sevilla (CSIC), Universidad de Sevilla, Avenida Américo Vespucio 49, 41092 Seville, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Yubero
authors.affiliation: Facultad de Medicina, Servicios Centrales de Investigación en Ciencias de la Salud, University of Cadiz, Dr. Marañon 3, 11002 Cadiz, Spain.
authors.forename: Juan L
authors.initials: JL
authors.lastname: Gonzalez-Caballero
authors.affiliation: Nanotechnology on Surfaces Laboratory, Instituto de Ciencia de Materiales de Sevilla (CSIC), Universidad de Sevilla, Avenida Américo Vespucio 49, 41092 Seville, Spain.
authors.forename: Agustin R
authors.initials: AR
authors.lastname: González-Elipe
authors.affiliation: Facultad de Medicina, Servicios Centrales de Investigación en Ciencias de la Salud, University of Cadiz, Dr. Marañon 3, 11002 Cadiz, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Vilches
authors.affiliation: Facultad de Medicina, Servicios Centrales de Investigación en Ciencias de la Salud, University of Cadiz, Dr. Marañon 3, 11002 Cadiz, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Salido
mesh_terms: D001704:Biopolymers / Q000737:chemistry*; D001861:Bone Regeneration; D002448:Cell Adhesion / Q000187:drug effects; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects; D011098:Polyglactin 910 / Q000737:chemistry; D012822:Silicon Dioxide / Q000138:chemical synthesis / Q000737:chemistry* / Q000627:therapeutic use; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D001704:Biopolymers; D011098:Polyglactin 910; D012822:Silicon Dioxide