title: Myogenic potential of canine craniofacial satellite cells.
abstract: The skeletal fibers have different embryological origin; the extraocular and jaw-closer muscles develop from prechordal mesoderm while the limb and trunk muscles from somites. These different origins characterize also the adult muscle stem cells, known as satellite cells (SCs) and responsible for the fiber growth and regeneration. The physiological properties of presomitic SCs and their epigenetics are poorly studied despite their peculiar characteristics to preserve muscle integrity during chronic muscle degeneration. Here, we isolated SCs from canine somitic [somite-derived muscle (SDM): vastus lateralis, rectus abdominis, gluteus superficialis, biceps femoris, psoas] and presomitic [pre-somite-derived muscle (PSDM): lateral rectus, temporalis, and retractor bulbi] muscles as myogenic progenitor cells from young and old animals. In addition, SDM and PSDM-SCs were obtained also from golden retrievers affected by muscular dystrophy (GRMD). We characterized the lifespan, the myogenic potential and functions, and oxidative stress of both somitic and presomitic SCs with the aim to reveal differences with aging and between healthy and dystrophic animals. The different proliferation rate was consistent with higher telomerase activity in PSDM-SCs compared to SDM-SCs, although restricted at early passages. SDM-SCs express early (Pax7, MyoD) and late (myosin heavy chain, myogenin) myogenic markers differently from PSDM-SCs resulting in a more efficient and faster cell differentiation. Taken together, our results showed that PSDM-SCs elicit a stronger stem cell phenotype compared to SDM ones. Finally, myomiR expression profile reveals a unique epigenetic signature in GRMD SCs and miR-206, highly expressed in dystrophic SCs, seems to play a critical role in muscle degeneration. Thus, miR-206 could represent a potential target for novel therapeutic approaches.
authors.affiliation: Department of Neuroscience and Imaging, University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Interuniversity Institute of Myology (IIM), University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Center for Excellence on Ageing (CeSI), G d'Annunzio Foundation , Chieti , Italy.
authors.forename: Rita Maria Laura
authors.initials: RM
authors.lastname: La Rovere
authors.affiliation: Interuniversity Institute of Myology (IIM), University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Translational Cardiomyology Laboratory, Stem Cell Biology and Embryology, Department of Development and Regeneration, KU Leuven , Leuven , Belgium.
authors.forename: Mattia
authors.initials: M
authors.lastname: Quattrocelli
authors.affiliation: Department of Neuroscience and Imaging, University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Interuniversity Institute of Myology (IIM), University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Center for Excellence on Ageing (CeSI), G d'Annunzio Foundation , Chieti , Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Pietrangelo
authors.affiliation: Department of Neuroscience and Imaging, University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Interuniversity Institute of Myology (IIM), University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Center for Excellence on Ageing (CeSI), G d'Annunzio Foundation , Chieti , Italy.
authors.forename: Ester Sara
authors.initials: ES
authors.lastname: Di Filippo
authors.affiliation: Interuniversity Institute of Myology (IIM), University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Department of Experimental Veterinary Sciences, Faculty of Veterinary Medicine, University of Padua , Padua , Italy.
authors.forename: Lisa
authors.initials: L
authors.lastname: Maccatrozzo
authors.affiliation: School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne , Lausanne , Switzerland.
authors.forename: Marco
authors.initials: M
authors.lastname: Cassano
authors.affiliation: Interuniversity Institute of Myology (IIM), University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Department of Comparative Biomedicine and Food Safety, University of Padua , Padua , Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Mascarello
authors.affiliation: Laboratoire de Neurobiologie, Ecole Nationale Vétérinaire d'Alfort , Maisons-Alfort , France.
authors.forename: Inès
authors.initials: I
authors.lastname: Barthélémy
authors.affiliation: Laboratoire de Neurobiologie, Ecole Nationale Vétérinaire d'Alfort , Maisons-Alfort , France.
authors.forename: Stephane
authors.initials: S
authors.lastname: Blot
authors.affiliation: Interuniversity Institute of Myology (IIM), University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Translational Cardiomyology Laboratory, Stem Cell Biology and Embryology, Department of Development and Regeneration, KU Leuven , Leuven , Belgium ; Department of Public Health, Experimental and Forensic Medicine, Division of Human Anatomy, University of Pavia , Pavia , Italy.
authors.forename: Maurilio
authors.initials: M
authors.lastname: Sampaolesi
authors.affiliation: Department of Neuroscience and Imaging, University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Interuniversity Institute of Myology (IIM), University "G. d'Annunzio" Chieti-Pescara , Chieti , Italy ; Center for Excellence on Ageing (CeSI), G d'Annunzio Foundation , Chieti , Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Fulle
keywords: differentiation; dystrophic muscle; microRNA; presomitic and somitic satellite cells