title: Monoclonal surface display SELEX for simple, rapid, efficient, and cost-effective aptamer enrichment and identification.
abstract: A novel method, monoclonal surface display SELEX (MSD-SELEX), has been designed for simple, rapid, efficient, and cost-effective enrichment and identification of aptamers from a library of monoclonal DNA-displaying beads produced via highly parallel single-molecule emulsion PCR. The approach was successfully applied for the identification of high-affinity aptamers that bind specifically to different types of targets, including cancer biomarker protein EpCAM and small toxin molecule aflatoxin B1. Compared to the conventional sequencing-chemical synthesis-screening work flow, MSD-SELEX avoids large-scale DNA sequencing, expensive and time-consuming DNA synthesis, and labor-intensive screening of large populations of candidates, thus offering a new approach for simple, rapid, efficient, and cost-effective aptamer identification for a wide variety of applications.
authors.affiliation: The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, The Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University , Xiamen 361005, P. R. China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yanling
authors.initials: Y
authors.lastname: Song
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zou
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.forename: Yuan
authors.initials: Y
authors.lastname: An
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
mesh_terms: D016604:Aflatoxin B1 / Q000737:chemistry; D000951:Antigens, Neoplasm / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015815:Cell Adhesion Molecules / Q000737:chemistry; D002460:Cell Line; D003362:Cost-Benefit Analysis; D000071858:Epithelial Cell Adhesion Molecule; D005434:Flow Cytometry; D006801:Humans; D016133:Polymerase Chain Reaction; D052156:SELEX Aptamer Technique
chemical_list: D000951:Antigens, Neoplasm; D052157:Aptamers, Nucleotide; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D016604:Aflatoxin B1