title: MicroRNA-429 induces tumorigenesis of human non-small cell lung cancer cells and targets multiple tumor suppressor genes.
abstract: Lung cancer is the major cause of cancer death globally. MicroRNAs are evolutionally conserved small noncoding RNAs that are critical for the regulation of gene expression. Aberrant expression of microRNA (miRNA) has been implicated in cancer initiation and progression. In this study, we demonstrated that the expression of miR-429 are often upregulated in non-small cell lung cancer (NSCLC) compared with normal lung tissues, and its expression level is also increased in NSCLC cell lines compared with normal lung cells. Overexpression of miR-429 in A549 NSCLC cells significantly promoted cell proliferation, migration and invasion, whereas inhibition of miR-429 inhibits these effects. Furthermore, we demonstrated that miR-429 down-regulates PTEN, RASSF8 and TIMP2 expression by directly targeting the 3'-untranslated region of these target genes. Taken together, our results suggest that miR-429 plays an important role in promoting the proliferation and metastasis of NSCLC cells and is a potential target for NSCLC therapy.
authors.affiliation: Department of Thoracic Surgery, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, Heilongjiang 150081, China.
authors.forename: Yaoguo
authors.initials: Y
authors.lastname: Lang
authors.affiliation: Department of Thoracic Surgery, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, Heilongjiang 150081, China.
authors.forename: Shidong
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Thoracic Surgery, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, Heilongjiang 150081, China.
authors.forename: Jianqun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Thoracic Surgery, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, Heilongjiang 150081, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Medical Oncology, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, Heilongjiang 150081, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Medical Oncology, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, Heilongjiang 150081, China.
authors.forename: Shoubo
authors.initials: S
authors.lastname: Cao
authors.affiliation: Department of Medical Oncology, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, Heilongjiang 150081, China. Electronic address: yuyan@hrbmu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D063646:Carcinogenesis / Q000187:drug effects / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018390:Gene Targeting / Q000379:methods; D016147:Genes, Tumor Suppressor / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D035683:MicroRNAs / Q000187:drug effects / Q000235:genetics*; D009361:Neoplasm Invasiveness
chemical_list: C546363:MIRN429 microRNA, human; D035683:MicroRNAs
keywords: Metastasis; MiR-429; Non-small cell lung cancer; PTEN; RASSF8; TIMP2