title: Short communication: Staphylococcus aureus isolated from colostrum of dairy heifers represent a closely related group exhibiting highly homogeneous genomic and antimicrobial resistance features.
abstract: In heifers, intramammary infections caused by Staphylococcus aureus affect milk production and udder health in the first and subsequent lactations, and can lead to premature culling. Not much is known about Staph. aureus isolated from heifers and it is also unclear whether or not these strains are readily transmitted between heifers and lactating herd mates. In this study, we compared phenotypic characteristics, spa types, and DNA microarray virulence and resistance gene profiles of Staph. aureus isolates obtained from colostrum samples of dairy heifers with isolates obtained from lactating cows. Our objective was to (1) characterize Staph. aureus strains associated with mastitis in heifers and (2) determine relatedness of Staph. aureus strains from heifers and lactating cows to provide data on transmission. We analyzed colostrum samples of 501 heifers and milk samples of 68 lactating cows within the same herd, isolating 48 and 9 Staph. aureus isolates, respectively. Staphylococcus aureus strains from heifers, lactating herd mates, and an unrelated collection of 78 strains from bovine mastitis milk of mature cows were compared. With 1 exception each, characterization of all strains from heifers and lactating cows in the same herd yielded highly similar phenotypic and genotypic results. The strains were Staphaurex latex agglutination test negative (Oxoid AG, Basel, Switzerland) and belonged to agr type II, CC705, and spa types tbl 2645 and t12926. They were susceptible to all antimicrobial agents tested. In contrast, the strains from mature cows in other herds were spread across different clonal complexes, spa types, and SplitsTree clusters (http://www.splitstree.org/), thus displaying a far higher degree of heterogeneity. We conclude that strains isolated from colostrum of heifers and mastitis milk of lactating cows in the same herd feature highly similar phenotypic and genomic characteristics, suggesting persistence of the organism during the first and potentially subsequent lactations or transmission between heifers and mature herd mates.
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.
authors.forename: Ueli
authors.initials: U
authors.lastname: Stalder
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.
authors.forename: Roger
authors.initials: R
authors.lastname: Stephan
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Corti
authors.affiliation: Forschungsinstitut für biologischen Landbau, 5070 Frick, Switzerland.
authors.forename: Maren
authors.initials: M
authors.lastname: Bludau
authors.affiliation: Forschungsinstitut für biologischen Landbau, 5070 Frick, Switzerland.
authors.forename: Ariane
authors.initials: A
authors.lastname: Maeschli
authors.affiliation: Forschungsinstitut für biologischen Landbau, 5070 Frick, Switzerland.
authors.forename: Peter
authors.initials: P
authors.lastname: Klocke
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland. Electronic address: sophia.johler@uzh.ch.
authors.forename: Sophia
authors.initials: S
authors.lastname: Johler
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D002417:Cattle; D003126:Colostrum / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D023281:Genomics; D060005:Genotyping Techniques; D007774:Lactation; D007841:Latex Fixation Tests; D008414:Mastitis, Bovine / Q000188:drug therapy / Q000382:microbiology; D008892:Milk / Q000382:microbiology; D013203:Staphylococcal Infections / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D013557:Switzerland
chemical_list: D000890:Anti-Infective Agents
keywords: Staphylococcus aureus; bovine mastitis; dairy heifer; genotyping