title: Characterization of carbapenem-resistant Acinetobacter baumannii in Brazil (2008-2011): countrywide spread of OXA-23-producing clones (CC15 and CC79).
abstract: The study investigated the genetic relationship of carbapenem-resistant Acinetobacter baumannii clinical isolated from inpatients during 2008-2011 from 11 Brazilian states. Antimicrobial susceptibility profile was determined by disc diffusion method and Etest. Polymerase chain reaction was applied for carbapenemase genes, and ISAba1. Isolates were subjected to pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) for molecular typing. Most of the isolates showed high resistance rates to antibiotics tested. The blaOXA-51-like gene was found in all isolates, and 146 (94.2%) isolates were positive for blaOXA-23-like. In the most OXA-23-producing isolates, the blaOXA-23-like gene was accompanied by ISAba1. A total of 146 OXA-23-producing isolates were clustered into 28 genotypes by PFGE. Molecular analysis by MLST identified 13 sequence types (STs). The most prevalent PFGE profiles were designated as ST15 (CC15), ST1 (CC1), and ST79 (CC79). This study showed the widespread of clonal complexes of A. baumannii harboring the blaOXA-23-like gene in different Brazilian states.
authors.affiliation: Laboratório de Pesquisa em Infecção Hospitalar (LAPIH), Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil 4365, Manguinhos, Rio de Janeiro, Brazil.
authors.forename: Thiago Pavoni Gomes
authors.initials: TP
authors.lastname: Chagas
authors.affiliation: Laboratório de Pesquisa em Infecção Hospitalar (LAPIH), Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil 4365, Manguinhos, Rio de Janeiro, Brazil.
authors.forename: Karyne Rangel
authors.initials: KR
authors.lastname: Carvalho
authors.affiliation: Laboratório de Pesquisa em Infecção Hospitalar (LAPIH), Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil 4365, Manguinhos, Rio de Janeiro, Brazil.
authors.forename: Ivson Cassiano
authors.initials: IC
authors.lastname: de Oliveira Santos
authors.affiliation: Laboratório de Pesquisa em Infecção Hospitalar (LAPIH), Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil 4365, Manguinhos, Rio de Janeiro, Brazil.
authors.forename: Ana Paula D'Alincourt
authors.initials: AP
authors.lastname: Carvalho-Assef
authors.affiliation: Laboratório de Pesquisa em Infecção Hospitalar (LAPIH), Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil 4365, Manguinhos, Rio de Janeiro, Brazil. Electronic address: marise@ioc.fiocruz.br.
authors.forename: Marise Dutra
authors.initials: MD
authors.lastname: Asensi
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics; D000483:Alleles; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001938:Brazil / Q000453:epidemiology; D015780:Carbapenems / Q000494:pharmacology*; D003428:Cross Infection; D062306:Geography, Medical; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D060885:Multiplex Polymerase Chain Reaction; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases
keywords: Acinetobacter baumannii; Brazil; Carbapenem resistance; MLST; OXA-23