title: Protective effects of rosmarinic acid against renal ischaemia/reperfusion injury in rats.
abstract: OBJECTIVE
  To investigate the potential protective effects of Rosmarinic acid (RA) on rats exposed to ischaemia/reperfusion renal injury.
  
  
  METHODS
  The prospective study was conducted at Abant Izzet Baysal University, Turkey, and comprised 21 male Spraque Dawley rats weighing 250-270g each. They were divided into three equal groups. Unilaterally nephrectomised rats were subjected to 60 minutes of left renal ischaemia followed by 60 minutes of reperfusion. Group 1 had sham-operated animals; group 2 had ischaemia/reperfusion untreated animals; and group 3 had ischaemia/reperfusion animals treated with rosmarinic acid. Serum creatinine, blood urea nitrogen, tissue malondialdehyde, glutathione peroxidase, superoxide dismutase and myeloperoxidase (MPO) activities, and light microscopic findings were evaluated. SPSS 17 was used for statistical analysis.
  
  
  RESULTS
  Treatment of rats with rosmarinic acid produced a reduction in the serum levels of creatinine and blood urea nitrogen compared to the other groups. However, no statistically significant difference was found. The levels of malondialdehyde and myeloperoxidase were decreased in the renal tissue of group 3, while glutathione peroxidose and superoxide dismutase levels remained unchanged. The injury score decreased in the treatment group rats compared to the untreated group. Rosmarinic acid significantly decreased focal glomerular necrosis, dilatation of Bowman's capsule, degeneration of tubular epithelium, necrosis in tubular epithelium, and tubular dilatation.
  
  
  CONCLUSIONS
  Rosmarinic acid prevented ischaemia/reperfusion injury in the kidneys by decreasing oxidative stress.
authors.forename: Hulya
authors.initials: H
authors.lastname: Ozturk
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Ozturk
authors.forename: Elcin Hakan
authors.initials: EH
authors.lastname: Terzi
authors.forename: Ufuk
authors.initials: U
authors.lastname: Ozgen
authors.forename: Arif
authors.initials: A
authors.lastname: Duran
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Uygun
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D002934:Cinnamates / Q000494:pharmacology*; D003404:Creatinine / Q000097:blood; D053630:Depsides / Q000494:pharmacology*; D007674:Kidney Diseases / Q000097:blood* / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009392:Nephrectomy; D018384:Oxidative Stress; D009195:Peroxidase / Q000097:blood; D011446:Prospective Studies; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood* / Q000517:prevention & control*; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D003404:Creatinine; D053630:Depsides; D008315:Malondialdehyde; D009195:Peroxidase; D000097123:Rosmarinic Acid