title: A cooperative jack model of random coil-to-elongation transition of the FH1 domain by profilin binding explains formin motor behavior in actin polymerization.
abstract: Filopodia are essential for the development of neuronal growth cones, cell polarity and cell migration. Their protrusions are powered by the polymerization of actin filaments linked to the plasma membrane, catalyzed by formin proteins. The acceleration of polymerization depends on the number of profilin-actins binding with the formin-FH1 domain. Biophysical characterization of the disordered formin-FH1 domain remains a challenge. We analyzed the conformational distribution of the diaphanous-related formin mDia1-FH1 bound with one to six profilins. We found a coil-to-elongation transition in the FH1 domain. We propose a cooperative "jack" model for the Formin-Homology-1 (FH1) domain of formins stacked by profilin-actins.
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore. Electronic address: zhaochen@nus.edu.sg.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore 138668, Singapore.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Christopher W V
authors.initials: CW
authors.lastname: Hogue
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore; Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore. Electronic address: dbslowbc@nus.edu.sg.
authors.forename: Boon Chuan
authors.initials: BC
authors.lastname: Low
mesh_terms: D000199:Actins / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry*; D003198:Computer Simulation; D000080865:Formins; D051379:Mice; D008958:Models, Molecular; D051304:Profilins / Q000737:chemistry*; D011485:Protein Binding; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011554:Pseudopodia / Q000502:physiology
chemical_list: D000199:Actins; D002352:Carrier Proteins; C491464:Diap1 protein, mouse; D000080865:Formins; D051304:Profilins
keywords: Actin filament; Conformational change; Disordered; Filopodium; Formin; “jack” Model