title: The prostaglandin synthase 2/cyclooxygenase 2 (PTGS2/ COX2) rs5277 polymorphism does not influence risk of colorectal cancer in an Iranian population.
abstract: BACKGROUND
  The prostaglandin-endoperoxide synthase 2 [PTGS2, commonly known as cyclooxygenase-2 (COX-2)] is an enzyme induced by proinflammatory stimuli that is often overexpressed in malignant tissue and involved in the synthesis of prostaglandins and thromboxanes, regulators of processes such as inflammation, cell proliferation, and angiogenesis, all relevant for cancer development. We investigated whether a functional genetic polymorphism, rs5277, in COX-2 may have a risk-modifying effect on sporadic colorectal cancer in an Iranian population.
  
  
  METHODS
  We conducted a case-control study on 167 patients with colorectal cancer and 197 cancer-free controls in Taleghani Hospital in Tehran, Iran, between 2007 and 2011. Peripheral blood samples of both groups were processed for DNA extraction and genotyping of the COX-2 gene polymorphism (rs5277) using PCR-RFLP. RFLP results were confirmed by direct sequencing. Logistic regression analysis was performed to calculate the adjusted odds ratio (OR) and 95% confidence interval (95% CI).
  
  
  RESULTS
  There was no significant difference in the distribution of COX-2 gene rs5277 polymorphism genotype and the allelic form, among CRC patients compared with the healthy control group (p: 0.867).
  
  
  CONCLUSIONS
  Our results suggest that rs5277 polymorphism in COX2 could not be a good prognostic indicator for patients with CRC.
authors.affiliation: Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran E-mail : Hamid.assadzadeh@gmail.com.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Khorshidi
authors.forename: Mahdi Montazer
authors.initials: MM
authors.lastname: Haghighi
authors.forename: Ehsan
authors.initials: E
authors.lastname: Nazemalhosseini Mojarad
authors.forename: Pedram
authors.initials: P
authors.lastname: Azimzadeh
authors.forename: Behzad
authors.initials: B
authors.lastname: Damavand
authors.forename: Mohsen
authors.initials: M
authors.lastname: Vahedi
authors.forename: Shohreh
authors.initials: S
authors.lastname: Almasi
authors.forename: Hamid Asadzadeh
authors.initials: HA
authors.lastname: Aghdaei
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Zali
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000235:genetics*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D051546:Cyclooxygenase 2 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007492:Iran; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human