title: The effects of florfenicol on the values of serum tumor necrosis factor-α and other biochemical markers in lipopolysaccharide-induced endotoxemia in brown trout.
abstract: The aim of the present study was to determine the effects of florfenicol on the expected changes in sTNF-α, damage markers of the liver and kidney, and the lipid metabolism parameters in endotoxemic brown trout. Ninety-six brown trout were included in this study. After six of the fish were reserved as the control group, the remaining 90 fish were divided equally into 3 groups as follows: LPS (2 mg/kg, IP), LPS (2 mg/kg, IP) + florfenicol (40 mg/kg, IM), and florfenicol (40 mg/kg, IM). Blood samples were obtained from the tail of the fish at 1.5, 3, 6, 10, and 24 hours. The levels of sTNF-α were determined by ELISA and biochemical markers were evaluated with an autoanalyzer. A significant increase was observed in the values of sTNF-α in the LPS and LPS + florfenicol groups (P < 0.05). Significant increases were found in the kidney and liver damage determinants in the LPS and LPS + florfenicol groups (P < 0.05). Irregular changes in the lipid metabolism parameters were observed in all the subgroups. In conclusion, florfenicol does not affect the increases of sTNF-α caused by LPS and does not prevent liver or kidney damage; at least, it can be said that florfenicol does not have any evident positive effects on the acute endotoxemia of fish.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selçuk, 42075 Konya, Turkey.
authors.forename: Ayse
authors.initials: A
authors.lastname: Er
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selçuk, 42075 Konya, Turkey.
authors.forename: Burak
authors.initials: B
authors.lastname: Dik
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D019446:Endotoxemia / Q000188:drug therapy* / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D007668:Kidney / Q000187:drug effects; D050356:Lipid Metabolism; D008070:Lipopolysaccharides / Q000737:chemistry*; D008099:Liver / Q000187:drug effects; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000494:pharmacology; D014337:Trout; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D000900:Anti-Bacterial Agents; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C035534:florfenicol; D013839:Thiamphenicol