title: Recombinant form of mammalian gp91(phox) is active in the absence of p22(phox).
abstract: The flavocytochrome b558 of the phagocyte NADPH oxidase complex comprises two membrane proteins, a glycosylated gp91phox and a non-glycosylated p22phox. Gp91phox contains all of the redox carriers necessary to reduce molecular oxygen to superoxide using NADPH. The capacity of gp91phox to produce superoxide in the absence of its membrane partner p22phox has been little studied. In the present study, we have generated in Pichia pastoris for the first time an active form of bovine gp91phox able to carry out the entire NADPH oxidase activity in the absence of p22phox. Collected information on the maturation and the activity of the recombinant gp91phox and the participation of individual cytosolic subunits in the active complex allowed us to propose, in the absence of p22phox, an unconventional stabilized complex compared with the heterodimer.
authors.affiliation: *Laboratoire de Chimie Physique, Université Paris Sud, UMR8000 CNRS, Bat. 350, 91405 Orsay Cedex, France.
authors.forename: Aymen
authors.initials: A
authors.lastname: Ezzine
authors.affiliation: *Laboratoire de Chimie Physique, Université Paris Sud, UMR8000 CNRS, Bat. 350, 91405 Orsay Cedex, France.
authors.forename: Hager
authors.initials: H
authors.lastname: Souabni
authors.affiliation: *Laboratoire de Chimie Physique, Université Paris Sud, UMR8000 CNRS, Bat. 350, 91405 Orsay Cedex, France.
authors.forename: Tania
authors.initials: T
authors.lastname: Bizouarn
authors.affiliation: *Laboratoire de Chimie Physique, Université Paris Sud, UMR8000 CNRS, Bat. 350, 91405 Orsay Cedex, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Baciou
mesh_terms: D000818:Animals; D002417:Cattle; D003573:Cytochrome b Group / Q000235:genetics / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D008081:Liposomes; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D003573:Cytochrome b Group; D008081:Liposomes; D008565:Membrane Proteins; D011994:Recombinant Proteins; C036888:cytochrome b558; D019255:NADPH Oxidases