title: Genome-wide survey and expression analysis of calcium-dependent protein kinase in Gossypium raimondii.
abstract: Calcium-dependent protein kinases (CDPKs) are one of the largest protein kinases in plants and participate in different physiological processes through regulating downstream components of calcium signaling pathways. In this study, 41 CDPK genes, from GrCPK1 to GrCPK41, were identified in the genome of the diploid cotton, Gossypium raimondii. The phylogenetic analysis indicated that all these genes were divided into four subgroups and members within the same subgroup shared conserved exon-intron structures. The expansion of GrCPKs family in G. raimondii was due to the segmental duplication events, and the analysis of Ka/Ks ratios implied that the duplicated GrCPKs had mainly undergone strong purifying selection pressure with limited functional divergence. The cold-responsive elements in promoter regions were detected in the majority of GrCPKs. The expression analysis of 11 selected genes showed that GrCPKs exhibited tissue-specific expression patterns and the expression of GrCPKs were induced or repressed by cold treatment. These observations would lay an important foundation for functional and evolutionary analysis of CDPK gene family in Gossypium species.
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan.
authors.forename: Muhammad Khan
authors.initials: MK
authors.lastname: Daud
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Shuijin
authors.initials: S
authors.lastname: Zhu
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D020440:Gene Duplication; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020131:Genes, Duplicate; D017343:Genes, Plant; D014644:Genetic Variation; D018745:Genome, Plant; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics*; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D012275:Oryza / Q000235:genetics; D010802:Phylogeny; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D036226:Seedlings / Q000201:enzymology / Q000235:genetics; D013696:Temperature; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases