title: Genetic mapping of the rice resistance-breaking gene of the brown planthopper Nilaparvata lugens.
abstract: Host plant resistance has been widely used for controlling the major rice pest brown planthopper (BPH, Nilaparvata lugens). However, adaptation of the wild BPH population to resistance limits the effective use of resistant rice varieties. Quantitative trait locus (QTL) analysis was conducted to identify resistance-breaking genes against the anti-feeding mechanism mediated by the rice resistance gene Bph1. QTL analysis in iso-female BPH lines with single-nucleotide polymorphism (SNP) markers detected a single region on the 10th linkage group responsible for the virulence. The QTL explained from 57 to 84% of the total phenotypic variation. Bulked segregant analysis with next-generation sequencing in F2 progenies identified five SNPs genetically linked to the virulence. These analyses showed that virulence to Bph1 was controlled by a single recessive gene. In contrast to previous studies, the gene-for-gene relationship between the major resistance gene Bph1 and virulence gene of BPH was confirmed. Identified markers are available for map-based cloning of the major gene controlling BPH virulence to rice resistance.
authors.affiliation: National Institute of Agrobiological Sciences, 1-2, O-washi, Tsukuba, Ibaraki 305-8634, Japan ttkoba@affrc.go.jp.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: National Institute of Agrobiological Sciences, 1-2, O-washi, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: National Institute of Agrobiological Sciences, 1-2, O-washi, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Suetsugu
authors.affiliation: National Institute of Agrobiological Sciences, 1-2, O-washi, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Seigo
authors.initials: S
authors.lastname: Kuwazaki
authors.affiliation: National Institute of Agrobiological Sciences, 1-2, O-washi, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hattori
authors.affiliation: Ubon Ratchathani Rice Research Center, PO Box 65, Muang, Ubon Ratchathani 34000, Thailand.
authors.forename: Jirapong
authors.initials: J
authors.lastname: Jairin
authors.affiliation: Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization, Kumamoto 861-1192, Japan.
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Sanada-Morimura
authors.affiliation: Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization, Kumamoto 861-1192, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D008040:Genetic Linkage; D006430:Hemiptera / Q000235:genetics*; D012275:Oryza / Q000235:genetics / Q000254:growth & development; D040641:Quantitative Trait Loci
keywords: brown planthopper; gene-for-gene interaction; host plant resistance; quantitative trait locus; rice