title: Genotyping, morphology and molecular characteristics of a lytic phage of Neisseria strain obtained from infected human dental plaque.
abstract: The lytic bacteriaphage (phage) A2 was isolated from human dental plaques along with its bacterial host. The virus was found to have an icosahedron-shaped head (60±3 nm), a sheathed and rigid long tail (∼175 nm) and was categorized into the family Siphoviridae of the order Caudovirales, which are dsDNA viral family, characterised by their ability to infect bacteria and are nonenveloped with a noncontractile tail. The isolated phage contained a linear dsDNA genome having 31,703 base pairs of unique sequence, which were sorted into three contigs and 12 single sequences. A latent period of 25 minutes and burst size of 24±2 particles was determined for the virus. Bioinformatics approaches were used to identify ORFs in the genome. A phylogenetic analysis confirmed the species inter-relationship and its placement in the family.
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Qassim University, Qassim, Saudi Arabia, aaljarbou@yahoo.com.
authors.forename: Ahmed N
authors.initials: AN
authors.lastname: Aljarbou
authors.forename: Mohamad
authors.initials: M
authors.lastname: Aljofan
mesh_terms: D001433:Bacteriolysis; D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D001483:Base Sequence; D016000:Cluster Analysis; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology*; D016679:Genome, Viral; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009343:Neisseria / Q000302:isolation & purification / Q000821:virology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017906:Siphoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D014771:Virion / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D004247:DNA