title: Differential expression analysis of Paralichthys olivaceus microRNAs in adult ovary and testis by deep sequencing.
abstract: MicroRNAs (miRNAs) play an important role in gonadal development and differentiation in fish. However, understanding of the mechanism of this process is hindered by our poor knowledge of miRNA expression patterns in fish gonads. In this study, miRNA libraries derived from adult gonads of Paralichthys olivaceus were generated by using next-generation sequencing (NGS) technology. Bioinformatics analysis was performed to distinguish mature miRNA sequences from two classes of small RNAs represented in the sequencing data. A total of 141 mature miRNAs were identified, in which 21 miRNAs were found in P. olivaceus for the first time. Variance and preference of miRNAs expression were concluded from the deep sequencing reads. Some miRNAs, such as pol-miR-143, pol-miR-26a and pol-let-7a were found with quite high expression levels in both gonads, while some exhibited a clear sex-biased expression in different gonad. Approximate 20.0% and 13.1% of the isolated miRNAs were preferentially expressed in the testis (FC<0.5) or ovary (FC>2), respectively. The identification and the preliminary analysis of the sex-biased expression of miRNAs in P. olivaceus gonads in our work by using NGS will provide us a basic catalog of miRNAs to facilitate future improvement and exploitation of sexual regulatory mechanisms in P. olivaceus.
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Department of Developmental Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Boulevard Dallas, TX 75390-9133, USA.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Wuhan Biogood Technology Co. Ltd., Wuhan Insititute of Biotechnology, B6-415 666 Gaoxin Road, East Lake High-Tech Development Zone, Wuhan 430075, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address: zlei0222@gmail.com.
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D019295:Computational Biology; D005260:Female; D005432:Flounder / Q000235:genetics* / Q000254:growth & development; D006066:Gonads / Q000254:growth & development / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D010053:Ovary / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism*
chemical_list: D035683:MicroRNAs
keywords: Deep sequencing; Ovary; Paralichthys olivaceus; Testis; miRNA