title: Structural comparison of lipophosphoglycan from Leishmania turanica and L. major, two species transmitted by Phlebotomus papatasi.
abstract: The lipophosphoglycan (LPG) of Leishmania major has a major role in the attachment to Phlebotomus papatasi midgut. Here, we investigated the comparative structural features of LPG of L. turanica, another species transmitted by P. papatasi. The mAb WIC 79.3, specific for terminal Gal(β1,3) side-chains, strongly reacted with L. turanica LPG. In contrast, L. turanica LPG was not recognized by arabinose-specific mAb 3F12. In conclusion, LPGs from L. major and L. turanica are similar, with the latter being less arabinosylated than L. major's. The high galactose content in L. turanica LPG is consistent with its predicted recognition by P. papatasi lectin PpGalec.
authors.affiliation: Department of Parasitology, Faculty of Science, Charles University, Prague 2 128 44, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Volf
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Paula M
authors.initials: PM
authors.lastname: Nogueira
authors.affiliation: Department of Parasitology, Faculty of Science, Charles University, Prague 2 128 44, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Myskova
authors.affiliation: Department of Biochemistry, University of Kentucky Medical Center, Lexington, KY 40536, USA.
authors.forename: Salvatore J
authors.initials: SJ
authors.lastname: Turco
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, 30190-002 Belo Horizonte, MG, Brazil. Electronic address: rsoares@cpqrr.fiocruz.br.
authors.forename: Rodrigo P
authors.initials: RP
authors.lastname: Soares
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D006028:Glycosphingolipids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007303:Insect Vectors / Q000469:parasitology*; D007891:Leishmania / Q000235:genetics / Q000378:metabolism*; D010691:Phlebotomus / Q000469:parasitology*; D013045:Species Specificity
chemical_list: D006028:Glycosphingolipids; C008290:lipophosphonoglycan
keywords: LPG; Leishmania turanica; Phlebotomus papatasi; Sand fly–Leishmania interaction