title: Expression pattern of Toll-like receptors (TLRs) in different organs and effects of lipopolysaccharide on the expression of TLR 2 and 4 in reproductive organs of female rabbit.
abstract: Toll-like receptors (TLRs) are vital for innate immunity, and they were expressed in various immune cells, tissues and organs. Moreover, TLRs specific expression pattern in different cells, tissue and organs have been confirmed to have correlation with the ability to resistance to pathogenic challenges. The present study aimed to determine the expression profiles and levels of TLR2, 3, 4, 5, 6, 8 and 10 in the lung, trachea, intestine, stomach, liver, spleen, uterine horn and body, cervix, ovary, oviduct and hypothalamus of female rabbits, and whether the expression level of TLR2 and 4 in the ovary, oviduct, uterine horn and body, and cervix were affected by lipopolyasaccharide (LPS). The tissues of the lung, trachea, intestine, stomach, liver, spleen, uterine horn and body, cervix, ovary, oviduct and hypothalamus were collected from four rabbits which didn't be treated as 0 h. 16 rabbits in LPS group were injected with LPS (according to 0.5mg/kg body weight) and 16 rabbits in control group were injected with saline (LPS carrier), hereafter the tissues of the uterine horn and body, cervix, ovary and oviduct from 32 rabbits were collected after 1.5, 3, 6, and 12h (n=4 each group) postinjection. The expression profiles of TLRs were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR), and expression level of TLRs were examined using quantitative real-time PCR (qRT-PCR). The results shown: TLR2, 3, 4, 5, 6, 8 and 10 were expressed in lung, trachea, intestine, stomach, liver, spleen, uterine horn and body, cervix, ovary, oviduct and hypothalamus of female rabbits, but their expression level had great difference in the same organs, and each TLR has different expression level in the different organs. After LPS-stimulation, the expression of TLR2 in the uterine body and horn was significantly higher than that in control group by 3h and 12h of postinjection (P<0.05) respectively. The expression of TLR4 in ovary and uterine body was significantly higher than that in control group by 3 and 12h of postinjection (P<0.05), and the expression of TLR4 in uterine body was greater than that in control by 3h postinjection (P<0.05). The results suggested LPS upregulated the expression of TLR2 and 4 in uterine body and horn, and the expression of TLR4 in ovary.
authors.affiliation: College of Animal Science & Technology, Sichuan Agricultural University, Yaan 625014, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Animal Science & Technology, Sichuan Agricultural University, Yaan 625014, China.
authors.forename: He
authors.initials: H
authors.lastname: Zibiao
authors.affiliation: College of Animal Science & Technology, Sichuan Agricultural University, Yaan 625014, China. Electronic address: zhm3000@163.com.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Ming
authors.affiliation: Institute of Animal Breeding and Genetic, Sichuan Agricultural University, Chengdu 6111130, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Shiyi
authors.affiliation: College of Animal Science & Technology, Sichuan Agricultural University, Yaan 625014, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Ruixia
authors.affiliation: College of Animal Science & Technology, Sichuan Agricultural University, Yaan 625014, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Jie
authors.affiliation: Institute of Animal Breeding and Genetic, Sichuan Agricultural University, Chengdu 6111130, China.
authors.forename: Lai
authors.initials: L
authors.lastname: SongJia
mesh_terms: D000818:Animals; D005260:Female; D005836:Genitalia, Female / Q000276:immunology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009928:Organ Specificity; D011817:Rabbits; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis / Q000235:genetics*; D015854:Up-Regulation / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4
keywords: Expression pattern; Lipopolysaccharide; Rabbit; Toll-like receptors