title: Cytotoxic effect of triterpenoids from the root bark of Hibiscus syriacus.
abstract: In this study, 4 new triterpenoids-3β- acetoxy-olean-11-en,28,13β-olide (1), 3β- acetoxy-11α,12α-epoxy-olean-28,13β-olide (2), 19α-epi-betulin (3), and 20, 28-epoxy-17β,19β-lupan-3β-ol (4)-and 12 known compounds, were isolated from the root bark of Hibiscus syriacus L. by using acetone extraction. Their structures were characterized by extensive spectroscopic analysis. To investigate cytotoxicity, A549 human lung cancer cells were exposed to the extract and the compounds identified from it. Significantly reduced cell viability was observed with betulin-3-caffeate (12) (IC50, 4.3 μM). The results of this study indicate that betulin-3-caffeate (12) identified from H. syriacus L. may warrant further investigation for potential as anticancer therapies.
authors.affiliation: Department of Biotechnology, National Formosa University, Yunlin, Taiwan.
authors.forename: Li-Shian
authors.initials: LS
authors.lastname: Shi
authors.affiliation: School of Pharmacy, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Chao-Hsuan
authors.initials: CH
authors.lastname: Wu
authors.affiliation: Department of Pharmacy Practice, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Te-Chun
authors.initials: TC
authors.lastname: Yang
authors.affiliation: Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Chen-Wen
authors.initials: CW
authors.lastname: Yao
authors.affiliation: School of Pharmacy, National Defense Medical Center, Taipei, Taiwan; Sinphar Pharmaceutical Co., Ltd., I-Lan, Taiwan.
authors.forename: Hang-Ching
authors.initials: HC
authors.lastname: Lin
authors.affiliation: School of Pharmacy, National Defense Medical Center, Taipei, Taiwan. Electronic address: wlchang@ndmctsgh.edu.tw.
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Chang
mesh_terms: D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D031584:Hibiscus / Q000737:chemistry*; D006801:Humans; D024301:Plant Bark / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*; D002109:Caffeic Acids; D000094062:Betulinic Acid
chemical_list: D014315:Triterpenes; C000596996:betulin-3-caffeate; D002109:Caffeic Acids; D000094062:Betulinic Acid
keywords: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (PubChem CID: 64965); Anticancer; Cytotoxicity; Hibiscus syriacus L; Triterpenoid; betulin (PubChem CID: 72326); betulin-3-caffeate (PubChem CID: 10153267); betulinic acid (PubChem CID: 64971); betulinic acid-3-caffeate (PubChem CID: 6439576); lupeol (PubChem CID: 73040); oleanolic acid (PubChem CID: 10494); oleanolic acid-3-acetate (PubChem CID: 151202); β-sitosterol (PubChem CID: 222284); β-sitosterol-3-O-β-D-glucoside (PubChem CID: 70699351)