title: Interaction of protein phosphatase inhibitors with membrane lipids assessed by surface plasmon resonance based binding technique.
abstract: The interaction of okadaic acid (OA), tautomycin (TM), microcystin-LR (MC-LR), cantharidin (CA), epigallocatechin-gallate (EGCG) and cyclosporin A (CsA), inhibitors of protein phosphatases, with liposome covered surfaces prepared from the lipid extracts of bovine brain, heart and liver was investigated by surface plasmon resonance (SPR) based binding technique. The SPR sensorgrams indicated reversible association or partial intercalation of the inhibitors with liposomes at 20°C or 37°C, respectively. Distinct lipid composition specificities were reflected in different saturation values of inhibitor binding in a decreasing order of liver>heart>>brain lipids. Assaying the effect of OA, TM, MC-LR, CA and EGCG on the activity of protein phosphatases in neuroblastoma B50, cardiomyoblast H9C2 and hepatocarcinoma HepG2 cells implied that the cell type specific association of phosphatase inhibitors with membrane lipids may influence their inhibitory potencies exerted on intact cells.
authors.affiliation: Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary; MTA-DE Cell Biology and Signaling Research Group, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Bálint
authors.initials: B
authors.lastname: Bécsi
authors.affiliation: Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Kiss
authors.affiliation: Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary; MTA-DE Cell Biology and Signaling Research Group, Faculty of Medicine, University of Debrecen, Debrecen, Hungary. Electronic address: erdodi@med.unideb.hu.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Erdődi
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods; D020349:Surface Plasmon Resonance / Q000379:methods
chemical_list: D008081:Liposomes; D008563:Membrane Lipids; D010749:Phosphoprotein Phosphatases
keywords: L1 sensor chip; Lipid immobilization; Protein phosphatase inhibitors; Protein phosphatase-1 (PP1) and -2A (PP2A); Surface plasmon resonance (SPR)