title: Aflatoxin B₁ and M₁ in milk.
abstract: The aflatoxin M1 (AFLAM1) is a mycotoxin that results from the hydroxylation of the aflatoxin B1 (AFLAB1). It contaminates the milk of animals fed with a diet containing its precursor. In this work, we determined the occurrence of AFLAB1 and AFLAM1 in milk, as well as the chromatographic conditions to quantify these mycotoxins. The extraction and quantification of AFLAB1 and AFLAM1 in naturally contaminated and artificially spiked milk samples which are produced and marketed in the state of RS were performed using the AOAC official method and UHPLC with fluorescence detection. We obtained a separation factor of 2.3 for AFLAB1 and AFLAM1 using a mobile phase consisting of 1% acetic acid:acetonitrile:methanol (55:10:35). The analytical curves had a wide linearity range and the limit of quantification (LOQm) concentrations of AFLAB1 and AFLAM1 were equal to 0.5 and 0.25 μg L(-1), respectively. Samples of pasteurized and ultra-high-temperature processed (UHT) milk showed natural contamination, and the levels for both aflatoxins ranged from 0.7 to 1.5 μg L(-1). Raw and concentrated milk samples only contained AFLAM1, with a maximum average concentration of 1.7 μg L(-1). These concentrations, higher than permitted by legislation, confirm the existence of a health risk, as well as highlight the relevance of searching for alternatives to reduce this contamination.
authors.affiliation: Laboratório de Ciência de Alimentos, Escola de Química e Alimentos, Universidade Federal do Rio Grande-FURG, Avenida Itália, km 8, Bairro Carreiros, Rio Grande do Sul 96203-900 Brazil. Electronic address: priscilascaglioni@gmail.com.
authors.forename: P T
authors.initials: PT
authors.lastname: Scaglioni
authors.affiliation: Programa de Pós Graduação em Tecnologia de Alimentos, Universidade Tecnológica Federal do Paraná-UTFPR, Avenida Brasil, Câmpus Medianeira, 4232 - Parque Independência, Medianeira, Parana 85884-000, Brazil.
authors.forename: T
authors.initials: T
authors.lastname: Becker-Algeri
authors.affiliation: Programa de Pós Graduação em Tecnologia de Alimentos, Universidade Tecnológica Federal do Paraná-UTFPR, Avenida Brasil, Câmpus Medianeira, 4232 - Parque Independência, Medianeira, Parana 85884-000, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Drunkler
authors.affiliation: Laboratório de Ciência de Alimentos, Escola de Química e Alimentos, Universidade Federal do Rio Grande-FURG, Avenida Itália, km 8, Bairro Carreiros, Rio Grande do Sul 96203-900 Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Badiale-Furlong
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000302:isolation & purification; D016607:Aflatoxin M1 / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003611:Dairy Products / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D013696:Temperature
chemical_list: D016607:Aflatoxin M1; D016604:Aflatoxin B1
keywords: Aflatoxin B(1); Aflatoxin M(1); Analytical method; Liquid chromatography; Milk