title: Efficient construction of unmarked recombinant mycobacteria using an improved system.
abstract: The genetic study of mycobacteria, such as Mycobacterium tuberculosis and Mycobacterium ulcerans, is hampered heavily by their slow growth. We have developed efficient, versatile, and improved genetic tools for constructing unmarked recombinant mycobacteria more rapidly including generating multiple mutants using the same antibiotic marker in both fast- and slow-growing mycobacteria.
authors.affiliation: State Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Respiratory Diseases, Department of Clinical Laboratory, Guangzhou Chest Hospital, China.
authors.forename: Yaoju
authors.initials: Y
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, China; School of Life Sciences, University of Science and Technology of China, China.
authors.forename: Tianzhou
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, China; School of Life Sciences, Anhui University, China.
authors.forename: Bangxing
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, China; School of Life Sciences, Anhui University, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Nursing and Health Sciences, Monash University, Australia.
authors.forename: Yue
authors.initials: Y
authors.lastname: Qu
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Nursing and Health Sciences, Monash University, Australia.
authors.forename: Trevor
authors.initials: T
authors.lastname: Lithgow
authors.affiliation: State Key Laboratory of Respiratory Diseases, Department of Clinical Laboratory, Guangzhou Chest Hospital, China.
authors.forename: Shouyong
authors.initials: S
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, China. Electronic address: zhang_tianyu@gibh.ac.cn.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D017353:Gene Deletion; D015870:Gene Expression; D023061:Gene Order; D017930:Genes, Reporter; D005818:Genetic Engineering; D009161:Mycobacterium / Q000235:genetics*; D020102:Mycobacterium smegmatis / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic
keywords: In-frame deletion; Mycobacteria; Recombineering; Unmarked gene disruption; Xer recombinase system