title: S100A4 promotes squamous cell laryngeal cancer Hep-2 cell invasion via NF-kB/MMP-9 signal.
abstract: OBJECTIVE
  S100A4 is a member of the S100 family of calcium-binding proteins, which possesses a wide range of biological functions, such as regulation of angiogenesis, cell survival, motility, and invasion. Here, we demonstrate for the first time a major role of S100A4 in the cell invasion properties of the human laryngeal squamous carcinoma cells (LSCC) and evaluated the mechanism.
  
  
  METHODS
  Cultured human LSCC cell line Hep-2 was overexpressed by transfection of pcDNA3.1-S100A4 plasmid. For this, cellular Hep-2 expression was quantified by Western blot analysis. Moreover, cell invasion and migration assays were performed. Furthermore, the impact of the S100A4 on NF-kB activity and MMP-9 expression was detected.
  
  
  RESULTS
  We found S100A4 potently promoted Hep-2 invasion, by increasing cell motility and matrix metalloproteinase-9 (MMP-9) production. The increase in MMP-9 production was mediated by activation of nuclear factor-kB transcriptional activity by S100A4. After MMP-9 and NF-kBp65 was inhibited by BB94 treatment and NF-kBp65 siRNA transfected, pcDNA3.1-S100A4 induced cell invasion and migration was decreased.
  
  
  CONCLUSIONS
  Our findings thus establish S100A4 as a major factor in the invasive abilities of Hep-2 cells.
authors.affiliation: Department of Head and Neck Tumor Surgery, Department of Radiotherapy, Linyi Cancer Institute Hospital, Linyi, China. cancerhly@126.com.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: C-w
authors.initials: CW
authors.lastname: Wang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; C498143:RELA protein, human; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; D051996:Transcription Factor RelA; C077591:S100A4 protein, human; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9