title: Clinical signs and bacterial communities of deciduous necrotic root canals detected by PCR-DGGE analysis: research association.
abstract: OBJECTIVE
  This study sought to investigate the possible association between clinical and radiographic data of the patients with the bacterial community profiles involved in cases of necrosis in primary root canals.
  
  
  METHODS
  Microbial community profiles for 25 samples from necrotic deciduous root canals were analyzed using the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting approach. These results were related to the clinical and radiographic data of these patients.
  
  
  RESULTS
  The analysis showed a large diversity of microbial communities in necrotic deciduous root canals. The statistical results pointed out that posterior and anterior teeth were associated with <20 bands and >20 bands in PCR-DGGE method, respectively. A relationship was verified between ages >4 years old and posterior teeth and, ages ≤4 years old and anterior teeth.
  
  
  CONCLUSIONS
  The data showed a polymicrobial community and pointed out the association of age with necrosis in anterior and posterior teeth.
authors.affiliation: Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Disciplina de Odontopediatria da FO-UFRJ, Cidade Universitária - CCS, Caixa Postal: 68066, CEP: 21941-971 Rio de Janeiro, RJ, Brazil. Electronic address: vicancio@ig.com.br.
authors.forename: Viviane Andrade Cancio
authors.initials: VA
authors.lastname: de Paula
authors.affiliation: Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Cidade Universitária, Av. Brigadeiro Trompowsky s/n, CEP: 21941-590 Rio de Janeiro, RJ, Brazil; Fellow by CAPES (Bolsista da CAPES) - Proc. n̊ BEX 9203 - CAPES Foundation, Ministry of Education of Brazil, Brasilia/DF 70040-020, Brazil; University of Groningen, Faculty of Mathematics and Natural Science, Microbial Ecology - Centre for Ecological and Evolutionary Studies, Nijenborgh 7, CEP: 9747AG, Groningen, Holanda, The Netherlands; Oral Medicine discipline, School of Dentistry, Graduate Program in Dentistry, Veiga de Almeida University, Rua Ibituruna, 108 - Maracanã, CEP: 20271-020 Rio de Janeiro, Brazil.
authors.forename: Dennis
authors.initials: D
authors.lastname: de Carvalho Ferreira
authors.affiliation: Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Cidade Universitária, Av. Brigadeiro Trompowsky s/n, CEP: 21941-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Fernanda Sampaio
authors.initials: FS
authors.lastname: Cavalcante
authors.affiliation: Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Cidade Universitária, Av. Brigadeiro Trompowsky s/n, CEP: 21941-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Flávia Lima
authors.initials: FL
authors.lastname: do Carmo
authors.affiliation: Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Cidade Universitária, Av. Brigadeiro Trompowsky s/n, CEP: 21941-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Alexandre Soares
authors.initials: AS
authors.lastname: Rosado
authors.affiliation: Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Disciplina de Odontopediatria da FO-UFRJ, Cidade Universitária - CCS, Caixa Postal: 68066, CEP: 21941-971 Rio de Janeiro, RJ, Brazil.
authors.forename: Laura Guimarães
authors.initials: LG
authors.lastname: Primo
authors.affiliation: Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Cidade Universitária, Av. Brigadeiro Trompowsky s/n, CEP: 21941-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Kátia Regina Netto
authors.initials: KR
authors.lastname: dos Santos
mesh_terms: D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D058645:Denaturing Gradient Gel Electrophoresis; D003786:Dental Pulp Cavity / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009336:Necrosis / Q000382:microbiology; D010483:Periapical Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012390:Root Canal Therapy; D014094:Tooth, Deciduous / Q000382:microbiology*
keywords: Deciduous root canals; Denaturing gradient gel electrophoresis; Endodontic infection; Primary teeth