title: The principal toxic glycosidic steroids in Cerbera manghas L. seeds: identification of cerberin, neriifolin, tanghinin and deacetyltanghinin by UHPLC-HRMS/MS, quantification by UHPLC-PDA-MS.
abstract: The toxicity of the sea mango (Cerbera manghas L.) is well known. The plant is ranked as one of the deadliest of the southern Asian coastline. Cardenolidic heterosides are responsible for the cardiotoxicity of trees of the Cerbera genus. We have identified and determined the concentration of the principal glycosidic steroids present in the seeds of sea mangos (Thailand). Drug screening of an extract of the seeds was carried out using ultra-high performance liquid chromatography coupled to photodiode array detection and mass spectrometry (UHPLC-PDA-MS) with quantification at 219nm. Identification was confirmed by UHPLC-HRMS. Deacetyltanghinin (m/z 549.3055±2ppm), neriifolin (m/z 535.3259±2ppm), tanghinin (m/z 591.3169±2ppm) and cerberin (577.3375±2ppm) were the most abundant glycosidic steroids present in the sea mango seeds. A seed of the dried ripe fruit had concentrations of 1209.1, 804.2, 621.4 and 285.9μg/g, respectively. A seed of the fresh unripe fruit had concentrations of 49.4, 47.0, 3.5 and 2.3μg/g.
authors.affiliation: Laboratoire LAT LUMTOX, 800 av. Marie Curie Z.I. Jean Jaurès, 07800 La Voulte-sur-Rhône, France. Electronic address: j.carlier@latlumtox.com.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Carlier
authors.affiliation: Laboratoire de toxicologie, Faculté de pharmacie de Lyon, 8 av. Rockefeller, 69373 Lyon, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guitton
authors.affiliation: Laboratoire LAT LUMTOX, Hôpital Desgenettes, 71 av. Rockefeller, 69003 Lyon, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Bévalot
authors.affiliation: Institut médico-légal, Faculté de médecine, 12 av. Rockefeller, 69008 Lyon, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Fanton
authors.affiliation: Laboratoire LAT LUMTOX, 800 av. Marie Curie Z.I. Jean Jaurès, 07800 La Voulte-sur-Rhône, France.
authors.forename: Yvan
authors.initials: Y
authors.lastname: Gaillard
mesh_terms: D000107:Acetylation; D029062:Apocynaceae / Q000737:chemistry*; D002298:Cardenolides / Q000032:analysis*; D002301:Cardiac Glycosides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D012639:Seeds / Q000737:chemistry
chemical_list: D002298:Cardenolides; D002301:Cardiac Glycosides; C069007:tanghinin; C040684:neriifolin; C006951:cerberin
keywords: Cardenolides; Cerbera manghas L.; Forensic toxicology; Ultra-high performance liquid chromatography–high resolution tandem mass spectrometry (UHPLC–HRMS/MS); Ultra-high performance liquid chromatography–photodiode array-mass spectrometry (UHPLC–PDA-MS)