title: Expression, purification, crystallization and preliminary X-ray analysis of the HER3-9E12 Fab complex.
abstract: 9E12 is a fully human immunoglobulin G1/κ monoclonal antibody that is specific for the epidermal growth factor receptor 3 (HER3), the overexpression of which has been detected in many tumour types and is associated with poor survival outcomes. To date, knowledge of the molecular mechanism for targeted antibodies that directly inhibit HER3 signalling is limited. Because knowledge of such therapeutic antibodies would help basic immunological therapeutics, structural insights into the HER3-9E12 Fab complex are important. Recombinant human HER3 and Fab fragments of the 9E12 antibody were cloned, expressed and crystallized, and crystallographic data sets were collected. The crystals belonged to space group P1, with unit-cell parameters a=74.4, b=98.6, c=99.6 Å, α=106.0, β=95.0, γ=102.5° and diffracted to a resolution of 2.1 Å.
authors.affiliation: Key Laboratory of Oncology, Cancer Center, Chinese PLA General Hospital and Chinese PLA Medical School, Beijing 100853, People's Republic of China.
authors.forename: Kecheng
authors.initials: K
authors.lastname: He
authors.affiliation: Department of Infectious Diseases, Beijing PLA 302 Hospital, Beijing 100039, People's Republic of China.
authors.forename: Ang
authors.initials: A
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory of Oncology, Cancer Center, Chinese PLA General Hospital and Chinese PLA Medical School, Beijing 100853, People's Republic of China.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeda
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D008969:Molecular Sequence Data; D020893:Receptor, ErbB-3 / Q000737:chemistry*; D015398:Signal Transduction
chemical_list: D007140:Immunoglobulin Fab Fragments; C581292:ERBB3 protein, human; D020893:Receptor, ErbB-3
keywords: HER3; targeted therapy