title: Telomere regulation during the cell cycle in fission yeast.
abstract: The fission yeast Schizosaccharomyces pombe has emerged as a useful model organism to study telomere maintenance mechanisms. In this chapter, we provide detailed protocols for quantitative ChIP and BrdU incorporation analyses to investigate how fission yeast telomeres are regulated during the cell cycle by utilizing cdc25-22 synchronized cell cultures.
authors.affiliation: Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, 900 S. Ashland (M/C 669), Chicago, IL, 60607, USA.
authors.forename: Bettina A
authors.initials: BA
authors.lastname: Moser
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Chang
authors.forename: Toru M
authors.initials: TM
authors.lastname: Nakamura
mesh_terms: D001973:Bromodeoxyuridine / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods; D002453:Cell Cycle; D047369:Chromatin Immunoprecipitation / Q000379:methods; D004271:DNA, Fungal / Q000032:analysis; D015151:Immunoblotting / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012568:Schizosaccharomyces / Q000166:cytology* / Q000378:metabolism; D016615:Telomere / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D001973:Bromodeoxyuridine