title: Effects of high fat diet, ovariectomy, and physical activity on leptin receptor expression in rat brain and white fat tissue.
abstract: OBJECTIVE
  To evaluate in a rat animal model whether ovariectomy, high fat diet (HFD), and physical activity in the form of running affect leptin receptor (Ob-R) distribution in the brain and white fat tissue compared to sham (Sh) surgery, standard diet (StD), and sedentary conditions.
  
  
  METHODS
  The study included 48 female laboratory Wistar rats (4 weeks old). Following eight weeks of feeding with standard or HFD, rats were subjected to either OVX or Sh surgery. After surgery, all animals continued StD or HFD for the next 10 weeks. During these 10 weeks, ovariectomy and Sh groups were subjected to physical activity or sedentary conditions. Free-floating immunohistochemistry and Western blot methods were carried out to detect Ob-R in the brain and adipose tissue.
  
  
  RESULTS
  StD-ovariectomy-sedentary group had a greater number of Ob-R positive neurons in lateral hypothalamic nuclei than StD-Sh-sedentary group. There was no difference in Ob-R positive neurons in arcuatus nuclei between all groups. Ob-R distribution in the barrel cortex was higher in HFD group than in StD group. Ob-R presence in perirenal and subcutaneous fat was decreased in StD-ovariectomy group.
  
  
  CONCLUSIONS
  HFD and ovariectomy increased Ob-R distribution in lateral hypothalamic nuclei, but there was no effect on arcuatus nuclei. Our results are first to suggest that HFD, ovariectomy, and physical activity affect Ob-R distribution in the barrel cortex, which might be correlated with the role of Ob-R in election of food in rats.
authors.forename: Senka
authors.initials: S
authors.lastname: Blažetić
authors.forename: Irena
authors.initials: I
authors.lastname: Labak
authors.forename: Barbara
authors.initials: B
authors.lastname: Viljetić
authors.forename: Marta
authors.initials: M
authors.lastname: Balog
authors.forename: Sandor G
authors.initials: SG
authors.lastname: Vari
authors.forename: Zora
authors.initials: Z
authors.lastname: Krivošíková
authors.forename: Martin
authors.initials: M
authors.lastname: Gajdoš
authors.forename: Patrícia
authors.initials: P
authors.lastname: Kramárová
authors.forename: Anton
authors.initials: A
authors.lastname: Kebis
authors.forename: Rosemary
authors.initials: R
authors.lastname: Vuković
authors.forename: Livia
authors.initials: L
authors.lastname: Puljak
authors.forename: Elizabeta
authors.initials: E
authors.lastname: Has-Schön
authors.affiliation: Marija Heffer, Department of Medical Biology, School of Medicine, University of Osijek, Josipa Huttlera 4, 31000 Osijek, Croatia, mheffer@mefos.hr.
authors.forename: Marija
authors.initials: M
authors.lastname: Heffer
mesh_terms: D052436:Adipose Tissue, White / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009043:Motor Activity / Q000502:physiology*; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D054411:Receptors, Leptin / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D054411:Receptors, Leptin