title: Genetic characterisation of Neospora caninum strains from clinical samples of zebuine foetuses obtained in abattoirs in Goiás, Brazil.
abstract: The Neospora caninum microsatellite markers were applied to clinical samples. Genotyping technology involving fluorescently labelled DNA fragment analysis was used in combination with DNA sequencing for markers with complex repetitive sequences. Nineteen DNA samples from 15 brains and four hearts of naturally infected non-aborted zebuine foetuses from abattoirs in Goiás, Brazil. N. caninum had been detected in these foetuses by nested-PCR of the internal transcribed spacer-1 rRNA region, and the samples were analysed using these microsatellites. Seven complete or nearly complete allele profiles were obtained from six foetuses. Three distinct profiles of N. caninum were identified in a unique microregion (Meia Ponte) of Goiás. Two alleles for the same marker were detected in a unique foetus that was probably infected with two different strains. A new allele for one of the microsatellites is described. The multilocus analysis performed here revealed a preliminary means of discriminating between individual strains according to their geographical origins. These are the first results that have been obtained regarding the molecular characterisation of strains of N. caninum from infected zebuine foetuses in South America and reveal for the first time that there are genotypic differences in the strains that are responsible for foetal transmission in zebuine foetuses.
authors.affiliation: Veterinary Parasitology Center/Veterinary and Zootechnics School, Federal University of Goiás, Caixa postal 131, CEP: 74001-970, Goiânia, Goiás, Brazil.
authors.forename: Paula Rogério Fernandes
authors.initials: PR
authors.lastname: Brom
authors.affiliation: SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Regidor-Cerrillo
authors.affiliation: SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Collantes-Fernández
authors.affiliation: SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: Ortega-Mora
authors.affiliation: Veterinary Parasitology Center/Veterinary and Zootechnics School, Federal University of Goiás, Caixa postal 131, CEP: 74001-970, Goiânia, Goiás, Brazil. Electronic address: marsalvet@gmail.com.
authors.forename: Marcelo Sales
authors.initials: MS
authors.lastname: Guimarães
authors.affiliation: Veterinary Parasitology Laboratory/Department of Microbiology, Immunology, Parasitology and Pathology, Institute of Public Health and Tropical Pathology, Federal University of Goiás, Rua 235, s/n, Setor Universitário, CEP 74605-050, Goiânia, Goiás, Brazil. Electronic address: acaetanos@hotmail.com.
authors.forename: Andréa Caetano
authors.initials: AC
authors.lastname: da Silva
mesh_terms: D000003:Abattoirs; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005260:Female; D005333:Fetus / Q000469:parasitology; D005838:Genotype; D005843:Geography; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D018895:Microsatellite Repeats / Q000235:genetics; D058885:Multilocus Sequence Typing / Q000662:veterinary; D018688:Neospora / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan
keywords: Bos indicus; Microsatellites; Multilocus genotyping; Neosporosis