title: Improving bone repair of femoral and radial defects in rabbit by incorporating PRP into PLGA/CPC composite scaffold with unidirectional pore structure.
abstract: In this study, a platelet-rich plasma poly(lactic-co-glycolic acid) (PRP-PLGA)/calcium phosphate cement (CPC) composite scaffold was prepared by incorporating PRP into PLGA/CPC scaffold with unidirectional pore structure, which was fabricated by the unidirectional freeze casting of CPC slurry and the following infiltration of PLGA. The results from in vitro cell experiments and in vivo implantation in femoral defects manifested that incorporation of PRP into PLGA/CPC scaffold improved in vitro cell response (cell attachment, proliferation, and differentiation), and markedly boosted bone formation, angiogenesis and material degradation. The incorporation of PRP into scaffold showed more outstanding improvement in osteogenesis as the scaffolds were used to repair the segmental radial defects, especially at the early stage. The new bone tissues grew along the unidirectional lamellar pores of scaffold. At 12 weeks postimplantation, the segmental radial defects treated with PRP-PLGA/CPC scaffold had almost recuperated, whereas treated with the scaffold without PRP was far from healed. Taken together, the PRP-PLGA/CPC scaffold with unidirectional pore structure is a promising candidate to repair bone defects at various sites.
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, People's Republic of China; Department of Biomedical Engineering, Guangzhou Medical University, Guangzhou, 510182, China.
authors.forename: Fupo
authors.initials: F
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Jiyan
authors.initials: J
authors.lastname: Li
authors.forename: Bomiao
authors.initials: B
authors.lastname: Lin
authors.forename: Yi
authors.initials: Y
authors.lastname: Ouyang
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Yuanyou
authors.initials: Y
authors.lastname: Xia
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Jiandong
authors.initials: J
authors.lastname: Ye
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D011100:Polyglycolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D016062:Porosity; D011817:Rabbits; D011884:Radius / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C523618:calcium phosphate polylactic-co-glycolic acid composite; D011100:Polyglycolic Acid; D000469:Alkaline Phosphatase
keywords: calcium phosphate cement; osteogenesis; platelet-rich plasma; segmental bone defect; unidirectional pore