title: Riboswitches based on kissing complexes for the detection of small ligands.
abstract: Biosensors derived from aptamers were designed for which folding into a hairpin shape is triggered by binding of the cognate ligand. These aptamers (termed aptaswitches) thus switch between folded and unfolded states in the presence and absence of the ligand, respectively. The apical loop of the folded aptaswitch is recognized by a second hairpin called the aptakiss through loop-loop or kissing interactions, whereas the aptakiss does not bind the unfolded aptaswitch. Therefore, the formation of a kissing complex signals the presence of the ligand. Aptaswitches were designed that enable the detection of GTP and adenosine in a specific and quantitative manner by surface plasmon resonance when using a grafted aptakiss or in solution by anisotropy measurement with a fluorescently labeled aptakiss. This approach is generic and can potentially be extended to the detection of any molecule for which hairpin aptamers have been identified, as long as the apical loop is not involved in ligand binding.
authors.affiliation: Univ. Bordeaux, IECB, Laboratoire ARNA, 2 rue Robert Escarpit, 33607 Pessac (France); Inserm U869, Laboratoire ARNA, 146 rue Léo Saignat, 33076 Bordeaux (France).
authors.forename: Guillaume
authors.initials: G
authors.lastname: Durand
authors.forename: Samuele
authors.initials: S
authors.lastname: Lisi
authors.forename: Corinne
authors.initials: C
authors.lastname: Ravelet
authors.forename: Eric
authors.initials: E
authors.lastname: Dausse
authors.forename: Eric
authors.initials: E
authors.lastname: Peyrin
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Toulmé
mesh_terms: D000241:Adenosine / Q000032:analysis; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000737:chemistry; D008024:Ligands; D009690:Nucleic Acid Conformation; D058928:Riboswitch; D020349:Surface Plasmon Resonance
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D008024:Ligands; D058928:Riboswitch; D000241:Adenosine
keywords: aptamers; biosensors; fluorescence anisotropy; nucleic acids; surface plasmon resonance