title: An alcohol oxidase of Phanerochaete chrysosporium with a distinct glycerol oxidase activity.
abstract: An intracellular alcohol oxidase (AOX) was isolated from the white-rot basidiomycete Phanerochaete chrysosporium (Pch), grown on l-lactate induction medium, and purified to electrophoretic homogeneity. The dimeric protein consisted of two identical 75kDa subunits. The open reading frame of 1,956bp resulted in a monomer consisting of 651 amino acids. The enzyme showed a pI at 5.4, a pH optimum of 9, a temperature optimum at 50°C, possessed putative conserved domains of the GMC superfamily, a FAD binding domain, and showed up to 86% homology to alcohol oxidase sequences of Gloeophyllum trabeum and Coprinopsis cinerea. As was shown for the first time for an AOX from a basidiomycete, not only methanol, but also lower primary alcohols and glycerol were accepted as substrates. An assay based on aldehyde dehydrogenase confirmed d-glyceraldehyde as the product of the reaction. A bioprocess based on this enzyme could alleviate the problems associated with the huge side-stream of glycerol occurring during the manufacture of biodiesel, yielding the green oxidant hydrogen peroxide.
authors.affiliation: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, D-30167, Hannover, Germany. Electronic address: Diana.linke@lci.uni-hannover.de.
authors.forename: Diana
authors.initials: D
authors.lastname: Linke
authors.affiliation: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, D-30167, Hannover, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Lehnert
authors.affiliation: Helmholtz Zentrum für Infektionsforschung, Inhoffenstrasse 7, D-38124 Braunschweig, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Nimtz
authors.affiliation: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, D-30167, Hannover, Germany.
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Berger
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D000429:Alcohol Oxidoreductases; D013401:Sugar Alcohol Dehydrogenases; C036131:glycerol oxidase; C019643:alcohol oxidase
keywords: Alcohol oxidase; Glycerol; Phanerochaete chrysosporium