title: Using "dioscorea batatas bean"-like silver nanoparticles based localized surface plasmon resonance to enhance the fluorescent signal of zinc oxide quantum dots in a DNA sensor.
abstract: We reported here the preparation of "dioscorea batatas bean"-like silver nanoparticles (AgNPs) and the unique structure provided the AgNPs good localized surface plasmon resonance (LSPR) property. In addition, zinc oxide quantum dots (ZnO QDs) were also synthesized and found with good fluorescent property. Furthermore, the ZnO QDs decorated exfoliated graphene oxide (EGO-ZnO) was prepared via electrostatic interaction. The named nanomaterials were applied in a LSPR-induced fluorescent DNA sensor. To fabricate the DNA sensor, the EGO-ZnO was modified on the silica glass as the supporter for the capture probe ssDNA, and the complementary ssDNA was labeled on the surface of the AgNPs. After the hybridization step by step, the AgNPs was fastened on the surface of the EGO-ZnO, and the fluorescent intensity of the EGO-ZnO increased as a result. The prepared DNA sensor enabled the target ssDNA to be detected in the concentration range of 10(-19)-10(-14)M, and the limit of detection was 4.3 × 10(-20)M.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Chu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shen
authors.affiliation: Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, PR China.
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ge
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: chm_yanm@126.com.
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
authors.affiliation: Cancer Research Center, Shandong Tumor Hospital, Jinan 250012, PR China.
authors.forename: Xianrang
authors.initials: X
authors.lastname: Song
mesh_terms: D004247:DNA / Q000032:analysis*; D027742:Dioscorea / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D045663:Quantum Dots / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D012834:Silver; D004247:DNA; D015034:Zinc Oxide
keywords: DNA sensor; Metal-enhanced fluorescence; Silver nanoparticle; Zinc oxide quantum dots