title: Immobilization of a Pleurotus ostreatus laccase mixture on perlite and its application to dye decolourisation.
abstract: In the present study, a crude laccase preparation from Pleurotus ostreatus was successfully immobilized on perlite, a cheap porous silica material, and tested for Remazol Brilliant Blue R (RBBR) decolourisation in a fluidized bed recycle reactor. Results showed that RBBR decolourisation is mainly due to enzyme action despite the occurrence of dye adsorption-related enzyme inhibition. Fine tuning of immobilization conditions allowed balancing the immobilization yield and the resulting rate of decolourisation, with the adsorption capacity of the solid biocatalyst. In the continuous lab scale reactor, a maximum conversion degree of 56.1% was achieved at reactor space-time of 4.2 h. Stability and catalytic parameters of the immobilized laccases were also assessed in comparison with the soluble counterparts, revealing an increase in stability, despite a reduction of the catalytic performances. Both effects are most likely ascribable to the occurrence of multipoint attachment phenomena.
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli Federico II, via Cynthia 4, 80126 Napoli, Italy.
authors.forename: Cinzia
authors.identifier: 0000-0001-5694-472X
authors.initials: C
authors.lastname: Pezzella
authors.affiliation: Istituto di Ricerche sulla Combustione, Consiglio Nazionale delle Ricerche, Piazzale V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: Maria Elena
authors.identifier: 0000-0002-5920-0096
authors.initials: ME
authors.lastname: Russo
authors.affiliation: Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, Piazzale V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: Antonio
authors.identifier: 0000-0002-8124-9419
authors.initials: A
authors.lastname: Marzocchella
authors.affiliation: Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, Piazzale V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: Piero
authors.initials: P
authors.lastname: Salatino
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli Federico II, via Cynthia 4, 80126 Napoli, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sannia
mesh_terms: D000327:Adsorption; D000537:Aluminum Oxide / Q000737:chemistry*; D001673:Biodegradation, Environmental; D003116:Color; D004396:Coloring Agents / Q000737:chemistry / Q000302:isolation & purification*; D045424:Complex Mixtures / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D042845:Laccase / Q000737:chemistry*; D020076:Pleurotus / Q000201:enzymology*; D012822:Silicon Dioxide / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000302:isolation & purification*; D018508:Water Purification / Q000379:methods*
chemical_list: D004396:Coloring Agents; D045424:Complex Mixtures; D004800:Enzymes, Immobilized; D014874:Water Pollutants, Chemical; C003076:Perlite; D012822:Silicon Dioxide; D042845:Laccase; D000537:Aluminum Oxide