title: A novel thermo-sensitive hydrogel based on thiolated chitosan/hydroxyapatite/beta-glycerophosphate.
abstract: In order to get a water-soluble in situ gel-forming system, a thiolated chitosan, chitosan-4-thio-butylamidine (CS-TBA) conjugate was synthesized and used to replace the unmodified chitosan in the application of the in situ gel-forming system. A novel thermo-sensitive hydrogel was prepared based on CS-TBA/hydroxyapatite (HA)/beta-glycerophosphate disodium (β-GP). The gel formation, rheological properties, morphology, degradation, cytotoxicity, as well as protein release process of the novel gel system were investigated in this study. The CS-TBA/HA/β-GP gel showed a higher storage modulus (G') and loss modulus (G″) and a decreased bovine serum albumin (BSA) release rate which was maintained the protein release for a longer time compared with the unmodified chitosan (CS)/HA/β-GP gel, due to the existence of thiol groups and/or disulfide bonds. The CS-TBA/HA/β-GP gel has a porous structure with a uniform distribution of nano-hydroxyapatite, an appropriate degradation rate and low cytotoxicity, showing potential applications in drug delivery and tissue engineering.
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Xujie
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Ultrasonic Diagnosis, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Qianli
authors.initials: Q
authors.lastname: Huang
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: Key Laboratory of Advanced Materials (MOE), School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. Electronic address: biomater@mail.tsinghua.edu.cn.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China. Electronic address: gzyubo@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
mesh_terms: D000818:Animals; D002417:Cattle; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods; D017886:Durapatite / Q000737:chemistry* / Q000378:metabolism; D005994:Glycerophosphates / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine; D013234:Stem Cells / Q000378:metabolism; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D005994:Glycerophosphates; D020100:Hydrogels; D012710:Serum Albumin, Bovine; D048271:Chitosan; D017886:Durapatite; C031463:beta-glycerophosphoric acid
keywords: Cytotoxicity; Degradation; Protein release; Thermo-sensitive hydrogel; Thiolated chitosan