title: Coronin3 regulates gastric cancer invasion and metastasis by interacting with Arp2.
abstract: Coronin3 expression is increased in gastric cancer (GC) tissues and can promote GC invasion and metastasis. However, the mechanisms underlying Coronin3 function in GC remain unclear. In this study, we aimed to explore the interacting molecules essential for the tumor-promoting effects of Coronin3 in GC. Using mass spectrometric analysis, functional studies, and immunohistochemistry, we found that Arp2 interacted with Coronin3, and ectopic expression of Arp2 promoted GC cell migration and invasion, while Arp2 knockdown suppressed whole-cell motility and attenuated the Coronin3-mediated upregulation of cell migration and invasion. In addition, both proteins correlated with the metastatic status of GC patients. Furthermore, survival analyses demonstrated that both Coronin3 and Arp2 correlated with overall GC patient survival, and the combination of Coronin3 and Arp2 most accurately predicted GC patient prognosis. Combined, these data demonstrate that Coronin3 can regulate GC invasion and metastasis through Arp2, and the combination of Coronin3 and Arp2 provides a potential marker for predicting GC prognosis.
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases; Xijing Hospital; Fourth Military Medical University; Xi'an, PR China; Department of Ultrasound Diagnostics; Tangdu Hospital; Fourth Military Medical University; Xi'an, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases; Xijing Hospital; Fourth Military Medical University; Xi'an, PR China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Shang
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases; Xijing Hospital; Fourth Military Medical University; Xi'an, PR China.
authors.forename: Gui
authors.initials: G
authors.lastname: Ren
authors.affiliation: The 88th Hospital of PLA; Tai'an, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases; Xijing Hospital; Fourth Military Medical University; Xi'an, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases; Xijing Hospital; Fourth Military Medical University; Xi'an, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases; Xijing Hospital; Fourth Military Medical University; Xi'an, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases; Xijing Hospital; Fourth Military Medical University; Xi'an, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases; Xijing Hospital; Fourth Military Medical University; Xi'an, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases; Xijing Hospital; Fourth Military Medical University; Xi'an, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism; D051377:Actin-Related Protein 2 / Q000235:genetics / Q000378:metabolism*; D000230:Adenocarcinoma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology; D011379:Prognosis; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D054378:Acetogenins
chemical_list: D015107:4-Butyrolactone; D051377:Actin-Related Protein 2; C495926:ACTR2 protein, human; C438920:coronin acetogenin; D054378:Acetogenins
keywords: Arp2; Coronin3; cell motility; cytoskeleton; gastric cancer