title: Immunochromatographic strip assay for the rapid and sensitive detection of Salmonella Typhimurium in artificially contaminated tomato samples.
abstract: This study was designed to confirm the applicability of a liposome-based immunochromatographic assay for the rapid detection of Salmonella enterica subsp. enterica serovar Typhimurium (Salmonella Typhimurium) in artificially contaminated tomato samples. To determine the detection limit and pre-enrichment incubation time (10, 12, and 18 h pre-enrichment in 1% buffered peptone water), the tests were performed with different cell numbers of Salmonella Typhimurium (3 × 10(0), 3 × 10(1), 3 × 10(2), and 3 × 10(3) CFU·mL(-1)) inoculated into 25 g of crushed tomato samples. The assay was able to detect as few as 30 Salmonella Typhimurium cells per 25 g of tomato samples (1.2 cells·g(-1)) after 12 h pre-enrichment incubation. Moreover, when the developed assay was compared with traditional morphological and biochemical culture-based methods as well as colloidal gold nanoparticle-based commercial test strips, the developed assay yielded positive results for the detection of Salmonella Typhimurium within a shorter period time. These findings confirm that the developed assay may have practical application for the sensitive detection of Salmonella Typhimurium in various food samples, including raw vegetables, with a relatively low detection limit and shorter analysis time.
authors.affiliation: Department of Food Science and Technology, Yeungnam University, 280 Daehak-ro, Gyeongsan-si, Gyeongsangbuk-do 712-749, Republic of Korea.
authors.forename: Shruti
authors.initials: S
authors.lastname: Shukla
authors.forename: Hyerim
authors.initials: H
authors.lastname: Leem
authors.forename: Jong-Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Myunghee
authors.initials: M
authors.lastname: Kim
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D058491:Bacterial Load; D002845:Chromatography / Q000379:methods* / Q000592:standards; D003101:Collodion; D003429:Cross Reactions; D005516:Food Microbiology; D007074:Immunoglobulin G / Q000276:immunology; D008081:Liposomes; D018551:Solanum lycopersicum / Q000382:microbiology*; D011934:Reagent Strips / Q000592:standards; D012478:Salmonella Food Poisoning / Q000382:microbiology / Q000517:prevention & control; D012486:Salmonella typhimurium / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D008081:Liposomes; D011934:Reagent Strips; D003101:Collodion
keywords: Salmonella Typhimurium; food safety; immunochromatographie; immunochromatography; liposome; sécurité alimentaire; tomate; tomato