title: Gene Expressing Difference in Sclerotial Formation of Morchella conica.
abstract: The difference of gene expression between sclerotia-producing and non-sclerotia-producing single spore isolates from Morchella conica were preliminary analyzed by mRNA differential display reverse transcription-polymerase chain reaction (RT-PCR) technique and 67 differential gene fragments were obtained. Fifty-eight of their second PCR products were cloned and sequenced. Thirteen special differential gene fragments related to sclerotial formation were validated by semi-quantitative RT-PCR. Some gene fragments had certain homologies with lipoprotein, cyclin-dependent kinase C-3, glycerophosphoryl diester phosphodiesterase, Rho GDP-dissociation inhibitor, gamma-aminobutyrate permease, OmpA family protein, Transcript antisense to ribosomal RNA protein, sodium-calcium exchange protein and keratin-associated proteins 5, 6. In addition, the putative protein of some DNA fragments had higher similarity with hypothetical protein-coding gene in NCBI database, as well as some were only putative gene fragments. All these fragments were speculated to be the functional gene associated with sclerotial formation in morel.
authors.affiliation: College of Life Sciences, Yunnan University, Kunming, 650091 Yunnan People's Republic of China ; Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650221 Yunnan People's Republic of China.
authors.forename: Li-Jiao
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650221 Yunnan People's Republic of China ; Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, 650221 Yunnan People's Republic of China ; Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9 Xueyun Road, Wuhua, Kunming, 650221 Yunnan People's Republic of China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Chai
authors.affiliation: Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650221 Yunnan People's Republic of China ; Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, 650221 Yunnan People's Republic of China ; Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9 Xueyun Road, Wuhua, Kunming, 650221 Yunnan People's Republic of China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Chen
authors.affiliation: Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650221 Yunnan People's Republic of China ; Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, 650221 Yunnan People's Republic of China ; Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9 Xueyun Road, Wuhua, Kunming, 650221 Yunnan People's Republic of China.
authors.forename: Xing-Qi
authors.initials: XQ
authors.lastname: Huang
authors.affiliation: Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650221 Yunnan People's Republic of China ; Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, 650221 Yunnan People's Republic of China ; Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9 Xueyun Road, Wuhua, Kunming, 650221 Yunnan People's Republic of China.
authors.forename: Yong-Chang
authors.initials: YC
authors.lastname: Zhao
keywords: Morchella conica; Sclerotia; Semi-quantitative RT-PCR; mRNA differential display