title: Rapid temperature-dependent wound closure following adipose fin clipping of Atlantic salmon Salmo salar L.
abstract: Three groups of Atlantic salmon were kept at a constant temperature of 4, 10 and 14 °C. The adipose fins were removed; six fish/group were sampled at 11 subsequent time points post-clipping. Samples were prepared for histopathological examination to study the course of re-epithelization. A score sheet was developed to assess the regeneration of epidermal and dermal cell types. Wounds were covered by a thin epidermal layer between 4 and 6 h post-clipping at 10 and 14 °C. In contrast, wound closure was completed between 6 and 12 h in fish held at a constant temperature of 4 °C. By 18 h post-clipping, superficial cells, cuboidal cells, prismatic basal cells and mucous cells were discernible in all temperature groups, rapidly progressing towards normal epidermal structure and thickness. Within the observation period, only minor regeneration was found in the dermal layers. A positive correlation between water temperature and healing rates was established for the epidermis. The rapid wound closure rate, epidermal normalization and the absence of inflammatory reaction signs suggest that adipose fin clipping under anaesthesia constitutes a minimally invasive method that may be used to mark large numbers of salmon presmolts without compromising fish welfare.
authors.affiliation: Centre of Epidemiology and Biostatistics, Faculty for Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, Oslo, Norway.
authors.forename: M
authors.initials: M
authors.lastname: Andrews
authors.forename: M
authors.initials: M
authors.lastname: Stormoen
authors.forename: H
authors.initials: H
authors.lastname: Schmidt-Posthaus
authors.forename: T
authors.initials: T
authors.lastname: Wahli
authors.forename: P J
authors.initials: PJ
authors.lastname: Midtlyng
mesh_terms: D058500:Animal Fins / Q000166:cytology / Q000502:physiology*; D000823:Animal Identification Systems / Q000592:standards / Q000662:veterinary*; D000818:Animals; D062326:Re-Epithelialization / Q000502:physiology*; D020317:Salmo salar / Q000502:physiology*; D013696:Temperature
keywords: animal welfare; epidermis; healing; integument; marking; skin