title: Ultrasonic disruption of fungal mycelia for efficient recovery of polysaccharide-protein complexes from viscous fermentation broth of a medicinal fungus.
abstract: High-intensity ultrasound (US) was applied to facilitate the extraction of intracellular and extracellular polysaccharide-protein complexes (PSPs) from the viscous mycelial fermentation broth of a medicinal fungus Cordyceps sinensis Cs-HK1. The US treatment caused the disruption of fungal mycelia, a dramatic reduction of the apparent broth viscosity, and the release of intracellular products into the liquid medium. The degree of mycelium disruption and the rate of intracellular product release were dependent on US power intensity, treatment period and biomass concentration of broth. The extraction or release kinetics of total water-soluble products and PSPs (yield Y versus time t) under the effect of US was fitted closely to the Elovich model Y=Yo+Y1 lnt and parabolic model Y=Yo+Y1t(½), respectively. Another interesting effect of the US treatment was a notable increase in the antioxidant cytoprotective activity of PSP against H2O2 induced cell death.
authors.affiliation: Department of Applied Biology and Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Cheung
authors.affiliation: Department of Applied Biology and Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors.forename: Xing-Xun
authors.initials: XX
authors.lastname: Liu
authors.affiliation: Department of Applied Biology and Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors.forename: Wing-Qiang
authors.initials: WQ
authors.lastname: Wang
authors.affiliation: Department of Applied Biology and Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. Electronic address: jian-yong.wu@polyu.edu.hk.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Wu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D018533:Biomass; D005591:Chemical Fractionation / Q000379:methods*; D032661:Cordyceps / Q000166:cytology* / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D007700:Kinetics; D025282:Mycelium / Q000166:cytology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012212:Rheology; D012995:Solubility; D013010:Sonication; D014783:Viscosity; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005656:Fungal Proteins; D011134:Polysaccharides; D014867:Water
keywords: Cell disruption; Kinetic model; Medicinal fungus; Mycelial broth; Polysaccharide–protein complex; Ultrasound