title: Core-shell structure microcapsules with dual pH-responsive drug release function.
abstract: We report dual pH-responsive microcapsules manufactured by combining electrostatic droplets (ESD) and microfluidic droplets (MFD) techniques to produce monodisperse core (alginate)-shell (chitosan) structure with dual pH-responsive drug release function. The fabricated core-shell microcapsules were size controllable by tuning the synthesis parameters of the ESD and MFD systems, and were responsive in both acidic and alkaline environment, We used two model drugs (ampicillin loaded in the chitosan shell and diclofenac loaded in the alginate core) for drug delivery study. The results show that core-shell structure microcapsules have better drug release efficiency than respective core or shell particles. A biocompatibility test showed that the core-shell structure microcapsules presented positive cell viability (above 80%) when evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results indicate that the synthesized core-shell microcapsules were a potential candidate of dual-drug carriers.
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Kaohsiung, Taiwan.
authors.forename: Chih-Hui
authors.initials: CH
authors.lastname: Yang
authors.forename: Chih-Yu
authors.initials: CY
authors.lastname: Wang
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.forename: Andrew H-J
authors.initials: AH
authors.lastname: Wang
authors.forename: Ching-Ju
authors.initials: CJ
authors.lastname: Hsiao
authors.forename: Zu-Yu
authors.initials: ZY
authors.lastname: Chen
authors.forename: Keng-Shiang
authors.initials: KS
authors.lastname: Huang
mesh_terms: D000464:Alginates / Q000737:chemistry; D000667:Ampicillin / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D004008:Diclofenac / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity; D065546:Drug Liberation; D004867:Equipment Design; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008956:Models, Chemical; D053769:Nanocapsules / Q000737:chemistry* / Q000633:toxicity
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D053769:Nanocapsules; D004008:Diclofenac; D000667:Ampicillin; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Core-shell; Drug carriers; Drug release; Dual pH-responsive