title: The distribution and common amino acid polymorphisms of human papillomavirus (HPV)-31 variants in 2700 women from Northern China.
abstract: To investigate the distribution of Human papillomavirus (HPV)-31 A, B and C variants as well as the common amino acid polymorphisms in Chinese women, all 14 HPV-31 positive cervical exfoliated cell specimens identified from a descriptive study including ∼2700 women from Northern China were analyzed. HPV-31 positive specimens were identified by Mass Spectrometry and the fragments of partial Long Control Region, E6 and E7 were amplified and directly sequenced or cloned into vector and then sequenced to confirm the variant information. HPV-31 prevalence in Northern Chinese female population was 0.52%. Six different sequences represented all 14 isolates, and these isolates were subsequently classified into variant lineage A (9), B (0) and C (5) by phylogenetic analysis. Five common amino acid polymorphism sites (2 in E6 and 3 in E7) and a novel non-synonymous mutation were detected in the current study. Our investigation suggested that HPV-31 was much less detected in Chinese women population than that in western countries. A and C variants were commonly detected while B variants were rarely detected in this population.
authors.affiliation: Laboratory of Genetics, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, P. R. China.
authors.forename: Mengfei
authors.initials: M
authors.lastname: Liu
authors.affiliation: Laboratory of Genetics, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, P. R. China.
authors.forename: Zhonghu
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Pathology, School of Medicine, University of Washington, Seattle, Washington, United States of America.
authors.forename: Longfu
authors.initials: L
authors.lastname: Xi
authors.affiliation: Laboratory of Genetics, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, P. R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: Laboratory of Genetics, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, P. R. China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Laboratory of Genetics, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Laboratory of Genetics, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, P. R. China.
authors.forename: Yaqi
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Laboratory of Genetics, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Ning
authors.affiliation: Laboratory of Genetics, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, P. R. China.
authors.forename: Chuanhai
authors.initials: C
authors.lastname: Guo
authors.affiliation: Anyang Cancer Hospital, Anyang, Henan, P. R. China.
authors.forename: Ruiping
authors.initials: R
authors.lastname: Xu
authors.affiliation: Anyang Cancer Hospital, Anyang, Henan, P. R. China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Laboratory of Genetics, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cai
authors.affiliation: Laboratory of Genetics, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ke
mesh_terms: D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000378:metabolism / Q000821:virology*; D002681:China / Q000453:epidemiology; D015331:Cohort Studies; D003710:Demography; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D058952:Human papillomavirus 31 / Q000145:classification / Q000235:genetics*; D006801:Humans; D013058:Mass Spectrometry; D008875:Middle Aged; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism; D050725:Papillomavirus E7 Proteins / Q000235:genetics / Q000378:metabolism; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D010802:Phylogeny
chemical_list: C572886:E6 protein, Human papillomavirus type 31; C502189:E7 protein, Human papillomavirus type 31; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins