title: The tomato phosphatidylinositol-phospholipase C2 (SlPLC2) is required for defense gene induction by the fungal elicitor xylanase.
abstract: The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol-phospholipase C (PI-PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated upon pathogen attack. We have previously shown that the fungal elicitor xylanase rapidly induces nitric oxide (NO), which is required for PI-PLCs activity and downstream defense responses in tomato cell suspensions. Here, we show that all six SlPLC genes are expressed in tomato cell suspensions. Treatment of the cells with xylanase induces an early increase in SlPLC5 transcript levels, followed by a raise of the amount of SlPLC2 transcripts. The production of NO is required to augment SlPLC5 transcript levels in xylanase-treated tomato cells. Xylanase also induces SlPLC2 and SlPLC5 transcript levels in planta. We knocked-down the expression of SlPLC2 and SlPLC5 by virus-induced gene silencing. We found that SlPLC2 is required for xylanase-induced expression of the defense-related genes PR1 and HSR203J.
authors.affiliation: Instituto de Investigaciones Biológicas - CONICET, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1245, CP 7600, Mar del Plata, Argentina.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Gonorazky
authors.affiliation: Instituto de Investigaciones Biológicas - CONICET, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1245, CP 7600, Mar del Plata, Argentina.
authors.forename: Leonor
authors.initials: L
authors.lastname: Ramirez
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abd-El-Haliem
authors.affiliation: Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Jack H
authors.initials: JH
authors.lastname: Vossen
authors.affiliation: Instituto de Investigaciones Biológicas - CONICET, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1245, CP 7600, Mar del Plata, Argentina.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Lamattina
authors.affiliation: Instituto de Investigaciones Biológicas - CONICET, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1245, CP 7600, Mar del Plata, Argentina.
authors.forename: Arjen
authors.initials: A
authors.lastname: ten Have
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Matthieu H A J
authors.initials: MH
authors.lastname: Joosten
authors.affiliation: Instituto de Investigaciones Biológicas - CONICET, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1245, CP 7600, Mar del Plata, Argentina. Electronic address: amlaxalt@mdp.edu.ar.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Laxalt
mesh_terms: D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000145:classification* / Q000201:enzymology* / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism
chemical_list: D010712:Phosphatidic Acids; D010940:Plant Proteins; D043364:Endo-1,4-beta Xylanases
keywords: Defense gene; Nitric oxide; Pathogen-associated molecular pattern; Phosphatidic acid; Phospholipid signaling