title: Analysis of oxidative stress status, catalase and catechol-O-methyltransferase polymorphisms in Egyptian vitiligo patients.
abstract: Vitiligo is the most common depigmentation disorder of the skin. Oxidative stress is implicated as one of the probable events involved in vitiligo pathogenesis possibly contributing to melanocyte destruction. Evidence indicates that certain genes including those involved in oxidative stress and melanin synthesis are crucial for development of vitiligo. This study evaluates the oxidative stress status, the role of catalase (CAT) and catechol-O-Methyltransferase (COMT) gene polymorphisms in the etiology of generalized vitiligo in Egyptians. Total antioxidant capacity (TAC) and malondialdehyde (MDA) levels as well as CAT exon 9 T/C and COMT 158 G/A polymorphisms were determined in 89 patients and 90 age and sex-matched controls. Our results showed significantly lower TAC along with higher MDA levels in vitiligo patients compared with controls. Meanwhile, genotype and allele distributions of CAT and COMT polymorphisms in cases were not significantly different from those of controls. Moreover, we found no association between both polymorphisms and vitiligo susceptibility. In conclusion, the enhanced oxidative stress with the lack of association between CAT and COMT polymorphisms and susceptibility to vitiligo in our patients suggest that mutations in other genes related to the oxidative pathway might contribute to the etiology of generalized vitiligo in Egyptian population.
authors.affiliation: Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Dina A
authors.initials: DA
authors.lastname: Mehaney
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Hebatallah A
authors.initials: HA
authors.lastname: Darwish
authors.affiliation: Dermatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Rehab A
authors.initials: RA
authors.lastname: Hegazy
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Mohammed M
authors.initials: MM
authors.lastname: Nooh
authors.affiliation: Dermatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Amira M
authors.initials: AM
authors.lastname: Tawdy
authors.affiliation: Dermatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Heba I
authors.initials: HI
authors.lastname: Gawdat
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Maha M
authors.initials: MM
authors.lastname: El-Sawalhi
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002374:Catalase / Q000235:genetics*; D002394:Catechol O-Methyltransferase / Q000235:genetics*; D003710:Demography; D004534:Egypt; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D014820:Vitiligo / Q000201:enzymology* / Q000235:genetics*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D008315:Malondialdehyde; D002374:Catalase; D002394:Catechol O-Methyltransferase