title: Expression of Angiotensin II Type 1 Receptor in Rat Bladder Smooth Muscle Cells in Response to a Streptzotocin Induced Diabetes Mellitus Model.
abstract: BACKGROUND
  The aim of this study was to investigate angiotensin II type 1 (AT1) receptors in rat bladder smooth muscle cells and alterations of AT1 receptors by diabetes mellitus and diuretic states.
  
  
  METHODS
  Diabetes and diuresis were induced in adult female rats by a single intraperitoneal injection of streptozotocin and feeding 5% sucrose in water. Cystometry was performed on control, diuretic, and diabetic rats at 2 and 8 weeks after treatment. Immunohistochemical staining was used to detect expression of AT1 receptors in the bladder smooth muscle cell membrane.
  
  
  CONCLUSIONS
  In diabetic rats, expression of AT1 receptors in the bladder smooth muscle cell membrane increased at 2 weeks and further increased at 8 weeks. The local renin-angiotensin system in the rat bladder might be activated by the continuous hyperglycemia caused by intraperitoneal injection of streptozotocin administration.
authors.affiliation: Division of Nephro-Urology, Department of Translational Medical Sciences Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Tobu
authors.affiliation: Department of Urology, Saga University Faculty of Medicine, Saga, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Noguchi
authors.affiliation: Division of Nephro-Urology, Department of Translational Medical Sciences Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Hatada
authors.affiliation: Division of Nephro-Urology, Department of Translational Medical Sciences Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Mori
authors.affiliation: Division of Nephro-Urology, Department of Translational Medical Sciences Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Matsuo
authors.affiliation: Division of Nephro-Urology, Department of Translational Medical Sciences Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Sakai
keywords: Angiotensin receptor; Bladder; Diabetes mellitus; Renin-angiotensin system