title: Laser desorption ionization mass spectrometry of peptides on a hybrid CHCA organic-inorganic matrix.
abstract: We report applications of new hybrid organic-inorganic silica based materials as laser desorption/ionization (LDI)-promoting surfaces for high-throughput identification of peptides. The driving force of our work was to design a new material composed of a conventional MALDI matrix covalently attached to silica with a high organic/inorganic ratio in order to improve the UV absorption by such LDI hybrid matrices. Amorphous CHCA-functionalized silica presenting an organic content up to 1.3 mmol g(-1) (around 40% in weight from TGA and elementary analysis measurements) gave very interesting LDI performances in terms of detection sensitivity as well as relative ionization discrepancy (spectral suppression) through the analyses of small synthetic peptide mixtures (550-1300 Da) taking CHCA and amorphous silica as model matrices for control experiments.
authors.affiliation: Institut des Biomolécules Max Mousseron, UMR 5247, Universités Montpellier 1 et 2, Bâtiment Chimie (17), Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France. enjalbal@univ-montp2.fr.
authors.forename: Clément
authors.initials: C
authors.lastname: Fleith
authors.forename: Sonia
authors.initials: S
authors.lastname: Cantel
authors.forename: Gilles
authors.initials: G
authors.lastname: Subra
authors.forename: Ahmad
authors.initials: A
authors.lastname: Mehdi
authors.forename: Jeremie
authors.initials: J
authors.lastname: Ciccione
authors.forename: Jean
authors.initials: J
authors.lastname: Martinez
authors.forename: Christine
authors.initials: C
authors.lastname: Enjalbal
mesh_terms: D000595:Amino Acid Sequence; D003373:Coumaric Acids / Q000138:chemical synthesis / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012822:Silicon Dioxide / Q000138:chemical synthesis / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D003373:Coumaric Acids; D017646:Organosilicon Compounds; D010455:Peptides; C007175:alpha-cyano-4-hydroxycinnamate; D012822:Silicon Dioxide