title: Complete sequencing of IncI1 sequence type 2 plasmid pJIE512b indicates mobilization of blaCMY-2 from an IncA/C plasmid.
abstract: Sequencing of pJIE512b, a 92.3-kb IncI1 sequence type 2 (ST2) plasmid carrying bla(CMY-2), revealed a bla(CMY-2) context that appeared to have been mobilized from an IncA/C plasmid by the insertion sequence IS1294. A comparison with published plasmids suggests that bla(CMY-2) has been mobilized from IncA/C to IncI1 plasmids more than once by IS1294-like elements. Alignment of pJIE512b with the only other available IncI1 ST2 plasmid revealed differences across the backbones, indicating variability within this sequence type.
authors.affiliation: Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, and The University of Sydney, Westmead, New South Wales, Australia.
authors.forename: Kaitlin A
authors.initials: KA
authors.lastname: Tagg
authors.affiliation: Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, and The University of Sydney, Westmead, New South Wales, Australia.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Iredell
authors.affiliation: Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, and The University of Sydney, Westmead, New South Wales, Australia sally.partridge@health.nsw.gov.au.
authors.forename: Sally R
authors.initials: SR
authors.lastname: Partridge
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015780:Carbapenems / Q000494:pharmacology; D016971:Citrobacter freundii / Q000235:genetics; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D020071:Interspersed Repetitive Sequences; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D016415:Sequence Alignment; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; C094163:IncA protein, bacteria; D010750:Phosphoproteins; C103022:beta-lactamase CMY-2; D001618:beta-Lactamases