title: Regulatory B cells present in lymph nodes draining a murine tumor.
abstract: In cancer, B cells have been classically associated with antibody secretion, antigen presentation and T cell activation. However, a possible role for B lymphocytes in impairing antitumor response and collaborating with tumor growth has been brought into focus. Recent reports have described the capacity of B cells to negatively affect immune responses in autoimmune diseases. The highly immunogenic mouse tumor MCC loses its immunogenicity and induces systemic immune suppression and tolerance as it grows. We have previously demonstrated that MCC growth induces a distinct and progressive increase in B cell number and proportion in the tumor draining lymph nodes (TDLN), as well as a less prominent increase in T regulatory cells. The aim of this research was to study B cell characteristics and function in the lymph node draining MCC tumor and to analyze whether these cells may be playing a role in suppressing antitumor response and favoring tumor progression. Results indicate that B cells from TDLN expressed increased CD86 and MHCII co-stimulatory molecules indicating activated phenotype, as well as intracellular IL-10, FASL and Granzyme B, molecules with regulatory immunosuppressive properties. Additionally, B cells showed high inhibitory upon T cell proliferation ex vivo, and a mild capacity to secrete antibodies. Our conclusion is that even when evidence of B cell-mediated activity of the immune response is present, B cells from TDLN exhibit regulatory phenotype and inhibitory activity, probably contributing to the state of immunological tolerance characteristic of the advanced tumor condition.
authors.affiliation: Laboratorio de Oncología Experimental, IMEX-CONICET-ANM, Academia Nacional de Medicina, Buenos Aires, Argentina. E-mail: magliocoandrea@yahoo.com.ar.
authors.forename: Andrea
authors.initials: A
authors.lastname: Maglioco
authors.forename: Damián G
authors.initials: DG
authors.lastname: Machuca
authors.forename: Gabriela
authors.initials: G
authors.lastname: Camerano
authors.forename: Héctor A
authors.initials: HA
authors.lastname: Costa
authors.forename: Raúl
authors.initials: R
authors.lastname: Ruggiero
authors.forename: Graciela I
authors.initials: GI
authors.lastname: Dran
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D060151:B-Lymphocytes, Regulatory / Q000276:immunology*; D002452:Cell Count; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D005434:Flow Cytometry; D007108:Immune Tolerance / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology* / Q000473:pathology; D008807:Mice, Inbred BALB C; D010641:Phenotype; D012509:Sarcoma / Q000276:immunology* / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm
keywords: B regulatory cells; tolerance; tumor immunity