title: Regulation of plant immunity through modulation of phytoalexin synthesis.
abstract: Soybean hairy roots transformed with the resveratrol synthase and resveratrol oxymethyl transferase genes driven by constitutive Arabidopsis actin and CsVMV promoters were characterized. Transformed hairy roots accumulated glycoside conjugates of the stilbenic compound resveratrol and the related compound pterostilbene, which are normally not synthesized by soybean plants. Expression of the non-native stilbenic phytoalexin synthesis in soybean hairy roots increased their resistance to the soybean pathogen Rhizoctonia solani. The expression of the AhRS3 gene resulted in 20% to 50% decreased root necrosis compared to that of untransformed hairy roots. The expression of two genes, the AhRS3 and ROMT, required for pterostilbene synthesis in soybean, resulted in significantly lower root necrosis (ranging from 0% to 7%) in transgenic roots than in untransformed hairy roots that had about 84% necrosis. Overexpression of the soybean prenyltransferase (dimethylallyltransferase) G4DT gene in soybean hairy roots increased accumulation of the native phytoalexin glyceollin resulting in decreased root necrosis.
authors.affiliation: Department of Crop Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana, IL 61801, USA. zernova@illinois.edu.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Zernova
authors.affiliation: Department of Crop Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana, IL 61801, USA. lygin@illinois.edu.
authors.forename: Anatoli V
authors.initials: AV
authors.lastname: Lygin
authors.affiliation: Department of Crop Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana, IL 61801, USA. mpawlow4@gmail.com.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Pawlowski
authors.affiliation: Department of Crop Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana, IL 61801, USA. curthill@illinois.edu.
authors.forename: Curtis B
authors.initials: CB
authors.lastname: Hill
authors.affiliation: United States Department of Agriculture (USDA), Agricultural Research Service, University of Illinois, 1101 W. Peabody Drive, Urbana, IL 61801, USA. ghartman@illinois.edu.
authors.forename: Glen L
authors.initials: GL
authors.lastname: Hartman
authors.affiliation: Department of Crop Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana, IL 61801, USA. widholm@illinois.edu.
authors.forename: Jack M
authors.initials: JM
authors.lastname: Widholm
authors.affiliation: Department of Crop Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana, IL 61801, USA. lozovaya@illinois.edu.
authors.forename: Vera V
authors.initials: VV
authors.lastname: Lozovaya
mesh_terms: D004122:Dimethylallyltranstransferase; D057865:Plant Immunity / Q000502:physiology*; D018517:Plant Roots / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D012232:Rhizoctonia / Q000472:pathogenicity; D012717:Sesquiterpenes / Q000378:metabolism*; D013025:Glycine max / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D000094162:Phytoalexins
chemical_list: D012717:Sesquiterpenes; D004122:Dimethylallyltranstransferase; D000094162:Phytoalexins