title: Optimization of medium composition for 3-hydroxycarboxylic acid production by Pseudomonas mendocina-biodegraded polyhydroxybutyrate.
abstract: We optimized the culture medium for 3-hydroxycarboxylic acid production by Pseudomonas mendocina DS-04-T-biodegraded polyhydroxybutyrate (PHB) using the Plackett-Burman design, steepest ascent method, and Box-Behnken design. The optimized concentrations of the constituents of the culture medium were as follows: PHB (7.57 g/L), NH4 Cl (5.0 g/L), KH2 PO4 (2.64 g/L), Na2 HPO4 ·12H2 O (12 g/L), MgSO4 ·7H2 O (0.5 g/L), and CaCl2 ·2H2 O (5 mg/L). The yield of 3-hydroxycarboxylic acid obtained using the optimized culture medium was 56.8 ± 1.64%, which was 2.5-fold higher than that obtained when the unoptimized culture medium was used.
authors.affiliation: College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, People's Republic of China.
authors.forename: Zhanyong
authors.initials: Z
authors.lastname: Wang
authors.forename: Hailong
authors.initials: H
authors.lastname: Mao
authors.forename: Huifang
authors.initials: H
authors.lastname: Liu
authors.forename: Tingting
authors.initials: T
authors.lastname: Su
authors.forename: Husheng
authors.initials: H
authors.lastname: Jiang
mesh_terms: D063566:Biodegradable Plastics / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D002264:Carboxylic Acids / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D044203:Pseudomonas mendocina / Q000254:growth & development / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D063566:Biodegradable Plastics; D002264:Carboxylic Acids; D003470:Culture Media; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: 3-hydroxycarboxylic acid; Pseudomonas mendocina; polyhydroxybutyrate