title: Assessment of baicalin in mouse blood by monoclonal antibody-based icELISA.
abstract: An indirect competitive enzyme-linked immunosorbent assay (icELISA) based on monoclonal antibaodies (MAb) was recently developed. This new method displays high sensitivity and accuracy, and is especially suitable for pharmacokinetic studies in small laboratory animals. This study aimed to develop an icELISA procedure for baicalin (BAL) quantitation in blood. We successfully developed the icELISA and applied in pharmacokinetic assays of Gegen Qinlian Decoction in mice. A linear correlation was obtained for BAL concentrations in the range from 34.69 to 2220.00 µg/L. The regression equation was y = 1.5557 - 0.4028log(C) with a correlation coefficient of 0.9936. Precision and accuracy of the icELISA method were evaluated by the variations between replicates from well to well (intra-assay) and plate to plate (inter-assay). The values obtained for these parameters were within the normal range (<15%). The recovery rates ranged from 98.93 to 126.78%, meeting the requirements for biological samples. Stability studies showed that BAL sample solutions were intact for 1 h, enough time for UV detection. However, long-term storage and especially freeze-thaw procedures were detrimental to BAL. The pharmacokinetic parameters derived from mouse experiments were as follows: area under the curves from time 0 to 48 h, 1876.15 ± 1108.14 mg h/L; mean maximum blood concentrations, 101.09 ± 31.53 mg/L; time of maximum concentration, 3.58 ± 2.88 h; mean residence time, 79.30 ± 61.21 h.
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Kong
authors.forename: Ye
authors.initials: Y
authors.lastname: Sun
authors.forename: Huibin
authors.initials: H
authors.lastname: Feng
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.forename: Huihua
authors.initials: H
authors.lastname: Qu
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D051379:Mice; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C542794:gegenqinlian; C038044:baicalin
keywords: baicalin; icELISA; mouse; pharmacokinetics; traditional Chinese medicine