title: Yeast phospholipid biosynthesis is linked to mRNA localization.
abstract: Regulation of the localization of mRNAs and local translation are universal features in eukaryotes and contribute to cellular asymmetry and differentiation. In Saccharomyces cerevisiae, localization of mRNAs that encode membrane proteins requires the She protein machinery, including the RNA-binding protein She2p, as well as movement of the cortical endoplasmic reticulum (cER) to the yeast bud. In a screen for ER-specific proteins necessary for the directional transport of WSC2 and EAR1 mRNAs, we have identified enzymes that are involved in phospholipid metabolism. Loss of the phospholipid methyltransferase Cho2p, which showed the strongest impact on mRNA localization, disturbs mRNA localization, as well as ER morphology and segregation, owing to an increase in the amount of cellular phosphatidylethanolamine (PtdEtn). Mislocalized mRNPs containing She2p colocalize with aggregated cER structures, suggestive of the entrapment of mRNA and She2p by the elevated PtdEtn level. This was confirmed by the elevated binding of She2p to PtdEtn-containing liposomes. These findings underscore the importance of ER membrane integrity in mRNA transport.
authors.affiliation: Interfaculty Institute of Biochemistry, Universität Tübingen, 72076 Tübingen, Germany.
authors.forename: Orit
authors.initials: O
authors.lastname: Hermesh
authors.affiliation: Interfaculty Institute of Biochemistry, Universität Tübingen, 72076 Tübingen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Genz
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Ido
authors.initials: I
authors.lastname: Yofe
authors.affiliation: Interfaculty Institute of Biochemistry, Universität Tübingen, 72076 Tübingen, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Sinzel
authors.affiliation: Interfaculty Institute of Biochemistry, Universität Tübingen, 72076 Tübingen, Germany.
authors.forename: Doron
authors.initials: D
authors.lastname: Rapaport
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Maya
authors.initials: M
authors.lastname: Schuldiner
authors.affiliation: Interfaculty Institute of Biochemistry, Universität Tübingen, 72076 Tübingen, Germany ralf.jansen@uni-tuebingen.de.
authors.forename: Ralf-Peter
authors.initials: RP
authors.lastname: Jansen
mesh_terms: D004721:Endoplasmic Reticulum / Q000378:metabolism*; D050356:Lipid Metabolism / Q000235:genetics; D008081:Liposomes / Q000378:metabolism; D050918:Phosphatidylethanolamine N-Methyltransferase / Q000235:genetics / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D034443:RNA Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D008081:Liposomes; D010714:Phosphatidylethanolamines; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C471175:SHE2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C483858:phosphatidylethanolamine; C494486:CHO2 protein, S cerevisiae; D050918:Phosphatidylethanolamine N-Methyltransferase
keywords: CHO2; SHE2; Saccharomyces cerevisiae; mRNA localization; phospholipids