title: Simultaneous determination of five major compounds in the traditional medicine Pyeongwee-San by high performance liquid chromatography-diode array detection and liquid chromatography-mass spectrometry/mass spectrometry.
abstract: BACKGROUND
  Pyeongwee-San (PWS) has been widely used for treating acute gastritis, chronic, and gastritis.
  
  
  OBJECTIVE
  In this paper, simultaneous determination of five compounds (naringin, hesperidin, glycyrrhizin, atractylenolide III, and magnolol) from traditional medicine PWS using the high performance liquid chromatography (HPLC) was established for quality control.
  
  
  METHODS
  Optimum separations were obtained with a SHISEIDO C18 reverse-phase column by gradient elution with 0.1% Trifluoroacetic acid (TFA) water-acetonitrile as the mobile phase. The flow rate was 1 mL/min and detection wavelength was set at 205 nm and 250 nm. Validation of the analytical method was evaluated by linearity, precision, and accuracy test.
  
  
  RESULTS
  The calibration curves were linear over the established range with R (2) > 0.9978. The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.09 to 0.43 and 0.27 to 1.29 μg/mL. The method exhibited intra-day and inter-day precision range between 0.01-1.86% and 0.04-0.35% respectively. The recoveries of five compounds in PWS were in the range between 93.18-106.40%, and 0.20-1.51%. The application of this method was identified through the successful analysis of five compounds in 12 batches of PWS. In addition, identification of five compounds was confirmed by a liquid chromatography method and mass spectrometry.
  
  
  CONCLUSIONS
  The HPLC method was could be accomplished to the quality control and stable experiment for the preparations consisted of five major compounds.
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Korea.
authors.forename: Bohyoung
authors.initials: B
authors.lastname: Lee
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Korea.
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Korea.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Yun
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Korea.
authors.forename: Jiwoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Korea.
authors.forename: Min Rye
authors.initials: MR
authors.lastname: Eom
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Korea ; Research Institute of Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
keywords: High performance liquid chromatography-diode array detection; Pyeongwee-San; liquid chromatography-mass spectrometry/mass spectrometry; quality control; validation