title: Selective extraction of catecholamines by packed fiber solid-phase using composite nanofibers composing of polymeric crown ether with polystyrene.
abstract: For the first time, electrospun composite nanofibers comprising polymeric crown ether with polystyrene (PCE-PS) have been used for the selective extraction of catecholamines - dopamine (DA), norepinephrine (NE) and epinephrine (E) - prior to their analysis by high-performance liquid chromatography-electrochemical detection. Using a minicartridge packed with PCE-PS composite nanofibers, the target compounds were extracted effectively from urine samples to which diphenylborinic acid 2-aminoethyl ester was added as a complexing reagent. The extracted catecholamines could be liberated from the fiber by the addition of acetic acid. A good linearity was observed for catecholamines in the range of 2.0-200 ng mL(-1) (NE, E and DA). The detection limits of catecholamines (signal-to-noise ratio = 3) were 0.5 ng mL(-1) (NE), 0.2 ng mL(-1) (E) and 0.2 ng mL(-1) (DA), respectively. Under the optimized conditions, the absolute recoveries of the above three catecholamines were 90.6% (NE), 88.5% (E) and 94.5% (DA). The repeatability of extraction performance was from 5.4 to 9.2% (expressed as relative standard deviation). Our results indicate that the proposed method could be used for the determination of NE, E and DA in urine.
authors.affiliation: School of Public Health, Tianjin Medical University, Tianjin, 300070, China; Key Laboratory of Child Development and Learning Science (Ministry of Education), Research Center for Learning Science, Southeast University, Nanjing, 210096, China.
authors.forename: Li-Qin
authors.initials: LQ
authors.lastname: Chen
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Jian-Song
authors.initials: JS
authors.lastname: Qu
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Deng
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Kang
mesh_terms: D002395:Catecholamines / Q000302:isolation & purification* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D043844:Crown Ethers / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D057139:Nanofibers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D002395:Catecholamines; D043844:Crown Ethers; D011137:Polystyrenes
keywords: catecholamine; electrospun nanofiber; polymeric crown ether; solid-phase extraction