title: [ABO blood group genotyping based on amplification refractory mutation system].
abstract: OBJECTIVE
  To develop a rapid, cost-effective and reliable method for ABO genotyping.
  
  
  METHODS
  Six pairs of primers were designed for the amplification refractory mutation system (ARMS). Thirty peripheral blood DNA samples, derived from Chinese Han unrelated individuals, were genotyped by ARMS. The results were compared with the corresponding data obtained from serological methods and DNA sequencing.
  
  
  RESULTS
  The ABO genotyping results using ARMS were perfectly in line with the serological methods and DNA sequencing.
  
  
  CONCLUSIONS
  The method developed and optimized in this study is able to accurately and rapidly detect 28 kinds of ABO genotypes, indicative of a new approach applicable to clinical practice.
authors.affiliation: College of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: National Engineering Research Center for Miniaturized Detection Systems, Xi'an 710069, China.
authors.forename: Juanli
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Transfusion Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, China.
authors.forename: Jiangcun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Transfusion Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.affiliation: College of Life Sciences, Northwest University, Xi'an 710069; National Engineering Research Center for Miniaturized Detection Systems, Xi'an 710069, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Hui
authors.affiliation: College of Life Sciences, Northwest University, Xi'an 710069; National Engineering Research Center for Miniaturized Detection Systems, Xi'an 710069, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000017:ABO Blood-Group System / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D000017:ABO Blood-Group System