title: Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea.
abstract: Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity. The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively. Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2. A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively. The immunological assay results demonstrated that TPS1-2, which consisted mainly of HG pectins, showed phagocytosis-enhancing activity in HL-60 cells.
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China. wanghuijun666666@163.com.
authors.forename: Huijun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Unilever R&D Shanghai, 66 Lin Xin Road, Linkong Economic Development Zone, Shanghai 200335, China. wgd992003@126.com.
authors.forename: Guodong
authors.initials: G
authors.lastname: Wei
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China. feilion_01@hotmail.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Unilever R&D Bangalore, 66 Main Road, Whitefield, Bangalore 560066, India. Gautam.Banerjee@unilever.com.
authors.forename: Gautam
authors.initials: G
authors.lastname: Banerjee
authors.affiliation: Unilever R&D Bangalore, 66 Main Road, Whitefield, Bangalore 560066, India. Joshi.manoj@unilever.com.
authors.forename: Manoj
authors.initials: M
authors.lastname: Joshi
authors.affiliation: Department of Complementary Medicine, Faculty of Science and Technology, University of Westminster, London W1W 6UW, UK. a.bligh@westminster.ac.uk.
authors.forename: S W Annie
authors.initials: SW
authors.lastname: Bligh
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China. shisongshan1978@126.com.
authors.forename: Songshan
authors.initials: S
authors.lastname: Shi
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China. 15297626906@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Lian
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China. fhwei_love@126.com.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Fan
authors.affiliation: Unilever R&D Shanghai, 66 Lin Xin Road, Linkong Economic Development Zone, Shanghai 200335, China. wgd992003@126.com.
authors.forename: Xuelan
authors.initials: X
authors.lastname: Gu
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China. shunchunwang@126.com.
authors.forename: Shunchun
authors.initials: S
authors.lastname: Wang
mesh_terms: D002460:Cell Line; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008745:Methylation; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects; D013662:Tea / Q000737:chemistry*
chemical_list: D007155:Immunologic Factors; D013662:Tea; D010368:Pectins; C003181:polygalacturonic acid