title: Crystallization and preliminary crystallographic analysis of defective pollen wall (DPW) protein from Oryza sativa.
abstract: The defective pollen wall (dpw) gene of Oryza sativa encodes a fatty acid reductase (DPW) which plays important roles in primary fatty alcohol synthesis. DPW catalyzes the synthesis of 1-hexadecanol. The enzyme shows a higher specificity for palmitoyl-ACP than for palmitoyl-CoA as the substrate, and can only use NADPH as the cofactor. To gain an understanding of the molecular mechanism underlying the reaction catalyzed by DPW, the gene encoding DPW without the N-terminal 80 amino acids (DPWΔ80) was cloned into pET-28a vector and was overexpressed in Escherichia coli. DPWΔ80 was purified to homogeneity and screened for crystallization. DPWΔ80 in complex with NADPH produced crystals that diffracted X-rays to a resolution of 3.4 Å. The crystals belonged to space group P6₁ or P6₅, with unit-cell parameters a=b=222.8, c=114.0 Å, α=β=90, γ=120°.
authors.affiliation: State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, Henan University, Kaifeng 475004, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Laboratory of Structural Biology and MOE Laboratory of Protein Science, School of Medicine and Life Sciences, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Plant Science, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Suo
authors.affiliation: Laboratory of Structural Biology and MOE Laboratory of Protein Science, School of Medicine and Life Sciences, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Liming
authors.initials: L
authors.lastname: Yan
authors.affiliation: Institute of Plant Science, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, Henan University, Kaifeng 475004, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Miao
mesh_terms: D003460:Crystallization; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry*
chemical_list: D010940:Plant Proteins
keywords: DPW; Oryza sativa; fatty acid reductase