title: Comparison of the efficiency of transplantation of bone marrow multipotent mesenchymal stromal cells cultured under normoxic and hypoxic conditions and their conditioned media on the model of acute lung injury.
abstract: The therapeutic efficiency of intravenous injection of rat bone marrow multipotent mesenchymal stromal cells grown under conditions of normoxia and hypoxia (3% O2) and conditioned media from these cultures were compared on the rat model of acute lung injury induced by intraperitoneal injection of lipopolysaccharide. The best therapeutic efficiency was demonstrated by cells grown under hypoxic conditions. The effect of conditioned media was less pronounced and did not depend on the culturing conditions.
authors.affiliation: N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation; Federal Research Center of Specialized Health Care and Medical Technologies, Federal Medical-Biological Agency of the Russian Federation, Moscow, Russia, rubenchail@yandex.ru.
authors.forename: R K
authors.initials: RK
authors.lastname: Chailakhyan
authors.forename: A V
authors.initials: AV
authors.lastname: Aver'yanov
authors.forename: F G
authors.initials: FG
authors.lastname: Zabozlaev
authors.forename: P A
authors.initials: PA
authors.lastname: Sobolev
authors.forename: A V
authors.initials: AV
authors.lastname: Sorokina
authors.forename: D A
authors.initials: DA
authors.lastname: Akul'shin
authors.forename: Yu V
authors.initials: YV
authors.lastname: Gerasimov
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015687:Cell Hypoxia; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D000860:Hypoxia / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D007274:Injections, Intraperitoneal; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D017077:Culture Media, Conditioned; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D010100:Oxygen