title: Microfluidic in-reservoir pre-concentration using a buffer drain technique.
abstract: Pre-concentration methods are essential for detecting low concentrations of influenza virus in biological samples from patients. Here, we describe a new method for draining buffer from solution in the reservoir of a microfluidic device to increase the concentration of virus in the reservoir. Viruses were captured in the reservoir by an ion depletion barrier from connected ion selective microfluidic channels. 75 μl of buffer was successfully drained from a 100 μl sample, resulting in a 4-fold increase in influenza hemagglutinin concentration in the reservoir. The volume of the final concentrated sample was suitable for detection of influenza hemagglutinin by the enzyme-linked immunosorbent assay, demonstrating the usefulness of the developed platform for enhanced sensitivity of virus detection in a conventional analysis.
authors.affiliation: School of Mechanical Engineering, Korea University, Seongbuk, Seoul 136-701, Korea. sidchung@korea.ac.kr.
authors.forename: Junghyo
authors.initials: J
authors.lastname: Yoon
authors.forename: Youngkyu
authors.initials: Y
authors.lastname: Cho
authors.forename: Sewoon
authors.initials: S
authors.lastname: Han
authors.forename: Chae Seung
authors.initials: CS
authors.lastname: Lim
authors.forename: Jeong Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Seok
authors.initials: S
authors.lastname: Chung
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000295:instrumentation*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D055664:Electrochemical Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D004867:Equipment Design; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000032:analysis*; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D057230:Limit of Detection; D008422:Materials Testing; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009994:Osmolar Concentration; D010539:Permeability; D066330:Printing, Three-Dimensional; D011933:Reagent Kits, Diagnostic; D013499:Surface Properties; D013997:Time Factors
chemical_list: D004129:Dimethylpolysiloxanes; D005465:Fluorocarbon Polymers; C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D008567:Membranes, Artificial; D011933:Reagent Kits, Diagnostic; C040402:perfluorosulfonic acid; C013830:baysilon