title: Is there any relationship between gene expression of tumor antigens and CD4+ T cells in multiple myeloma?
abstract: OBJECTIVE
  The present study aimed at correlating the expression of cancer/testis antigens (CTAs) with the expression of genes related to tumor-infiltrating T cells.
  
  
  METHODS
  MAGE-C1/CT-7, MAGEA3/6, NY-ESO-1, LAGE-1 and GAGE expression were evaluated in 46 bone marrow multiple myeloma (MM) aspirates by RT-PCR. Expression of FOXP3/CTLA4 and RORyt, as markers for Tregs and Th17 cells, respectively, was investigated by quantitative PCR.
  
  
  RESULTS
  MAGEC1/CT7 was expressed in 66% of MM samples. We did not find correlation between the presence of single CTA and expression of CTLA4 or RORyt neither expression of CD4(+) T-cell markers and the number of CTA simultaneously expressed in the tumor. However, we did observe a correlation between the percentage of plasma cells and the number of CTAs expressed in the patients' bone marrow.
  
  
  CONCLUSIONS
  Although CTAs and immunomodulatory CD4(+) T cells represent potential targets for immunotherapy in MM, we did not find association among expression of such genes in MM.
authors.affiliation: Rua Doutor Diogo de Faria, 824, 4° andar, CEP 04037 04003, São Paulo, Brazil.
authors.forename: Walter Mt
authors.initials: WM
authors.lastname: Braga
authors.forename: Bruna R
authors.initials: BR
authors.lastname: da Silva
authors.forename: Veruska Lf
authors.initials: VL
authors.lastname: Alves
authors.forename: Adriana B
authors.initials: AB
authors.lastname: Bortoluzo
authors.forename: Djordje
authors.initials: D
authors.lastname: Atanackovic
authors.forename: Gisele Wb
authors.initials: GW
authors.lastname: Colleoni
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000276:immunology* / Q000473:pathology; D009363:Neoplasm Proteins / Q000276:immunology*; D010950:Plasma Cells / Q000276:immunology / Q000473:pathology
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins
keywords: Th17; Treg; cancer/testis antigen; multiple myeloma