title: Iron (FeII) chelation, ferric reducing antioxidant power, and immune modulating potential of Arisaema jacquemontii (Himalayan Cobra Lily).
abstract: This study explored the antioxidant and immunomodulatory potential of ethnomedicinally valuable species, namely, Arisaema jacquemontii of north-western Himalayan region. The tubers, leaves, and fruits of this plant were subjected to extraction using different solvents. In vitro antioxidant studies were performed in terms of chelation power on ferrous ions and FRAP assay. The crude methanol extract of leaves was found to harbour better chelating capacity (58% at 100 μ g/mL) and reducing power (FRAP value 1085.4 ± 0.11 μ MFe(3+)/g dry wt.) than all the other extracts. The crude methanol extract was thus further partitioned with solvents to yield five fractions. Antioxidant study of fractions suggested that the methanol fraction possessed significant chelation capacity (49.7% at 100 μ g/mL) and reducing power with FRAP value of 1435.4 μ M/g dry wt. The fractions were also studied for immune modulating potential where it was observed that hexane fraction had significant suppressive effect on mitogen induced T-cell and B-cell proliferation and remarkable stimulating effect on humoral response by 141% and on DTH response by 168% in immune suppressed mice as compared to the controls. Therefore, it can be concluded that A. jacquemontii leaves hold considerable antioxidant and immunomodulating potential and they can be explored further for the identification of their chemical composition for a better understanding of their biological activities.
authors.affiliation: School of Biotechnology, University of Jammu, Jammu, Jammu and Kashmir 180006, India.
authors.forename: Rasleen
authors.initials: R
authors.lastname: Sudan
authors.affiliation: School of Biotechnology, University of Jammu, Jammu, Jammu and Kashmir 180006, India.
authors.forename: Madhulika
authors.initials: M
authors.lastname: Bhagat
authors.affiliation: School of Biotechnology, University of Jammu, Jammu, Jammu and Kashmir 180006, India.
authors.forename: Sahil
authors.identifier: 0000-0002-7347-1968
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Department of Pharmacology, Indian Institute of Integrative Medicine, Jammu, Jammu and Kashmir 180001, India.
authors.forename: Jasvinder
authors.initials: J
authors.lastname: Singh
authors.affiliation: Department of Pharmacology, Indian Institute of Integrative Medicine, Jammu, Jammu and Kashmir 180001, India.
authors.forename: Anupurna
authors.initials: A
authors.lastname: Koul
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D031057:Arisaema / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D005296:Ferrous Compounds / Q000378:metabolism; D007111:Immunity, Cellular / Q000187:drug effects; D056724:Immunity, Humoral / Q000187:drug effects; D007155:Immunologic Factors / Q000494:pharmacology*; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D000432:Methanol / Q000737:chemistry; D051379:Mice; D064209:Phytochemicals / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D019163:Reducing Agents / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005296:Ferrous Compounds; D007155:Immunologic Factors; D007502:Iron Chelating Agents; D008070:Lipopolysaccharides; D064209:Phytochemicals; D010936:Plant Extracts; D019163:Reducing Agents; D003208:Concanavalin A; D007501:Iron; D000432:Methanol