title: Piperlongumine induces apoptosis and synergizes with cisplatin or paclitaxel in human ovarian cancer cells.
abstract: Piperlongumine (PL), a natural alkaloid from Piper longum L., possesses the highly selective and effective anticancer property. However, the effect of PL on ovarian cancer cells is still unknown. In this study, we firstly demonstrate that PL selectively inhibited cell growth of human ovarian cancer cells. Furthermore, PL notably induced cell apoptosis, G2/M phase arrest, and accumulation of the intracellular reactive oxidative species (ROS) in a dose- and time-dependent manner. Pretreatment with antioxidant N-acety-L-cysteine could totally reverse the PL-induced ROS accumulation and cell apoptosis. In addition, low dose of PL/cisplatin or paclitaxel combination therapies had a synergistic antigrowth effect on human ovarian cancer cells. Collectively, our study provides new therapeutic potential of PL on human ovarian cancer.
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Wenzhou Medical College, Shangcai Village South, Ouhai District, Wenzhou, Zhejiang 325000, China.
authors.forename: Li-Hua
authors.identifier: 0000-0001-7385-4961
authors.initials: LH
authors.lastname: Gong
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Wenzhou Medical College, Shangcai Village South, Ouhai District, Wenzhou, Zhejiang 325000, China.
authors.forename: Xiu-Xiu
authors.initials: XX
authors.lastname: Chen
authors.affiliation: Department of Gynecology, The Third Affiliated Hospital of Sun-Yat Sen University, Guangzhou, Guangdong 510632, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Cell Biology and Institute of Biomedicine, College of Life Science and Technology, Jinan University, Room 708, The 2nd Engineer and Scientific Building, 601 Huangpu RoadWest, Guangzhou, Guangdong 510632, China.
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Jiang
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Wenzhou Medical College, Shangcai Village South, Ouhai District, Wenzhou, Zhejiang 325000, China.
authors.forename: Shi-Shi
authors.initials: SS
authors.lastname: Pan
authors.affiliation: Department of Cell Biology and Institute of Biomedicine, College of Life Science and Technology, Jinan University, Room 708, The 2nd Engineer and Scientific Building, 601 Huangpu RoadWest, Guangzhou, Guangdong 510632, China.
authors.forename: Jian-Ge
authors.initials: JG
authors.lastname: Qiu
authors.affiliation: Department of Cell Biology and Institute of Biomedicine, College of Life Science and Technology, Jinan University, Room 708, The 2nd Engineer and Scientific Building, 601 Huangpu RoadWest, Guangzhou, Guangdong 510632, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Mei
authors.affiliation: Department of Cell Biology and Institute of Biomedicine, College of Life Science and Technology, Jinan University, Room 708, The 2nd Engineer and Scientific Building, 601 Huangpu RoadWest, Guangzhou, Guangdong 510632, China.
authors.forename: You-Qiu
authors.initials: YQ
authors.lastname: Xue
authors.affiliation: Department of Cell Biology and Institute of Biomedicine, College of Life Science and Technology, Jinan University, Room 708, The 2nd Engineer and Scientific Building, 601 Huangpu RoadWest, Guangzhou, Guangdong 510632, China.
authors.forename: Wu-Ming
authors.initials: WM
authors.lastname: Qin
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Wenzhou Medical College, Shangcai Village South, Ouhai District, Wenzhou, Zhejiang 325000, China.
authors.forename: Fei-Yun
authors.initials: FY
authors.lastname: Zheng
authors.affiliation: Department of Cell Biology and Institute of Biomedicine, College of Life Science and Technology, Jinan University, Room 708, The 2nd Engineer and Scientific Building, 601 Huangpu RoadWest, Guangzhou, Guangdong 510632, China.
authors.forename: Zhi
authors.identifier: 0000-0002-8328-0305
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Wenzhou Medical College, Shangcai Village South, Ouhai District, Wenzhou, Zhejiang 325000, China.
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Yan
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D004148:Dioxolanes / Q000494:pharmacology*; D004357:Drug Synergism; D005260:Female; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006801:Humans; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology; D017239:Paclitaxel / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D010881:Piperidones
chemical_list: D000111:Acetylcysteine; D002945:Cisplatin; D004148:Dioxolanes; D017239:Paclitaxel; D017382:Reactive Oxygen Species; C498077:piperlongumine; D010881:Piperidones