title: Leuconostoc mesenteroides SJRP55: A Bacteriocinogenic Strain Isolated from Brazilian Water Buffalo Mozzarella Cheese.
abstract: The production of bacteriocins by Leuconostoc mesenteroides represents an important opportunity for exploration of their potential use for industrial purpose. The antimicrobial compounds produced by L. mesenteroides subsp. mesenteroides SJRP55 strain were characterized and purified. Cell-free supernatant of Leuc. mesenteroides subsp. mesenteroides SJRP55 produced antibacterial compounds against Listeria spp. strains and not inhibiting against Lactobacillus spp. The antimicrobial substances were stable at high temperatures (100 °C for 2 h and 121 °C for 20 min) and low pH (pH 2-4) values, but sensitive to proteolytic enzymes and resistant to α-amylase, lipase and catalase enzymes. The optimal temperature for active peptides production was 25 °C. The antimicrobial compounds were purified by ammonium sulfate precipitation, affinity column and reverse-phase chromatography. Mass spectrometry and amino acids analyses showed that the bacteriocins were identical to mesentericin Y105 and B105. The producer strain's DNA analysis revealed presence of open reading frames possibly coding for virulence factors, such as enterococcal surface protein (esp), collagen adhesion (ace) and intrinsic vancomycin resistance (vanA); however, biogenic amines encoding genes were not observed. Leuc. mesenteroides subsp. mesenteroides SJRP55 is a promising biopreservative culture in fermented milk, and the purified bacteriocins can also be applied in food preservation.
authors.affiliation: Food Engineering and Technology Department, UNESP - Sao Paulo State University, Rua Cristovão Colombo, 2265, São José do Rio Preto, 15.054-000, Brazil.
authors.forename: Aline Teodoro
authors.initials: AT
authors.lastname: de Paula
authors.forename: Ana Beatriz
authors.initials: AB
authors.lastname: Jeronymo-Ceneviva
authors.forename: Luana Faria
authors.initials: LF
authors.lastname: Silva
authors.forename: Svetoslav Dimitrov
authors.initials: SD
authors.lastname: Todorov
authors.forename: Bernadette Dora Gombossy de Melo
authors.initials: BD
authors.lastname: Franco
authors.forename: Yvan
authors.initials: Y
authors.lastname: Choiset
authors.forename: Thomas
authors.initials: T
authors.lastname: Haertlé
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chobert
authors.forename: Xavier
authors.initials: X
authors.lastname: Dousset
authors.forename: Ana Lúcia Barretto
authors.initials: AL
authors.lastname: Penna
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry; D001938:Brazil; D002417:Cattle; D002611:Cheese / Q000382:microbiology*; D007934:Leuconostoc / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013058:Mass Spectrometry; D008892:Milk / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins