title: Irradiation enhances expression of cxcr4 in murine glioma cells via HIF-1α-independent pathway.
abstract: OBJECTIVE
  To investigate levels of chemokine (C-X-C motif) receptor 4 (cxcr4) mRNA and protein in X-irradiated glioma cells.
  
  
  METHODS
  Murine malignant glioma GL261 cells transfected with hypoxia-inducible factor (HIF)-1α miRNA or control miRNA were irradiated with X-radiation. Cxcr4 mRNA and protein were analysed using real-time reverse transcription-polymerase chain reaction and Western blot, respectively.
  
  
  RESULTS
  Levels of cxcr4 protein in GL261 cells increased in a radiation dose-dependent manner 48 h after 0, 5, 10 and 15 Gy X-irradiation. Irradiation of both HIF-1α knockdown cells and control cells resulted in a significant increase in cxcr4 mRNA levels, compared with nonirradiated cells, at 24 h after 5 Gy X-irradiation.
  
  
  CONCLUSIONS
  Irradiation enhances expression of cxcr4 in glioma cells via a HIF-1α-independent pathway.
authors.affiliation: Department of Radiotherapy, Cancer Centre, Qilu Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan, Shandong, China Brain Science Research Institute, Shandong University, Jinan, Shandong, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan, Shandong, China Brain Science Research Institute, Shandong University, Jinan, Shandong, China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Li
authors.affiliation: Affiliated Hospital, Shandong Academy of Medical Sciences, Jinan, Shandong, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan, Shandong, China Brain Science Research Institute, Shandong University, Jinan, Shandong, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Radiotherapy, Cancer Centre, Qilu Hospital, Shandong University, Jinan, Shandong, China drzhenyushao@hotmail.com.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Shao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000532:radiotherapy*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D019718:Receptors, CXCR4 / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C514936:CXCR4 protein, mouse; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D035683:MicroRNAs; D012333:RNA, Messenger; D019718:Receptors, CXCR4
keywords: CXCR4; Chemokine; glioma; neuropathology; radiotherapy