title: The pig as an experimental model for mid-dermal burns research.
abstract: This was a novel, prospective and interventional animal study designed to develop and evaluate a new infliction device for the experimental burn model. Four paired sets of contact burns measuring 36mm diameter were inflicted on the dorsum of an anesthetized pig using a stainless steel round bar heated up to 80-110°C. The bar was applied using a push-pull force gauge designed to control 1kgf mechanical force applied to the skin for a period of 20s. The left dorsum was used for macroscopic observation and the right dorsum was used for histopathological evaluation. A total of eight burns were covered with moist saline dressings and given daily treatments of xylocaine (lidocaine HCl) gel. This procedure was followed for a period of 24 days. Full-thickness biopsies were obtained for histologic analysis to determine the extent of injury. Statistical analysis showed a high correlation between the exposure temperature and histopathological assessment. The results found the depth of injury to the collagen (Seg1) correlated with the temperature (Ti) at which the burns was inflicted, Seg1=0.038Ti-2.57 (r=0.973, P<0.05). Also, the histological studies show a high correlation between the depth of collagen denaturation in wounds and the exposure temperature, Seg1=0.0268Ti-0.165 (r=0.991, P<0.05). This model is useful to assess more closely the therapeutic agents used for wound healing in experimental burn wounds.
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Integrated Chinese and Western Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Shi-Yuan
authors.initials: SY
authors.lastname: Sheu
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Chinese Internal Medicine, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Wang
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Chinese Medicine, Buddhist Tzu Chi General Hospital, Taichung Branch, Taichung, Taiwan.
authors.forename: Yuan-Tsung
authors.initials: YT
authors.lastname: Fu
authors.affiliation: Deng-Chuan Veterinary Hospital, Kaohsiung, Taiwan.
authors.forename: Sheng-Chuan
authors.initials: SC
authors.lastname: Lin
authors.affiliation: School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Yi-Chih
authors.initials: YC
authors.lastname: Lei
authors.affiliation: School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Jeng-Hao
authors.initials: JH
authors.lastname: Liao
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan.
authors.forename: Nou-Ying
authors.initials: NY
authors.lastname: Tang
authors.affiliation: School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan. Electronic address: tzongfu@ntu.edu.tw.
authors.forename: Tzong-Fu
authors.initials: TF
authors.lastname: Kuo
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan; Department of Biomedical Informatics, Asia University, Taichung, Taiwan. Electronic address: chyao@mail.cmu.edu.tw.
authors.forename: Chun-Hsu
authors.initials: CH
authors.lastname: Yao
mesh_terms: D000818:Animals; D001458:Bandages; D002056:Burns / Q000628:therapy*; D020405:Dermis / Q000293:injuries / Q000473:pathology*; D004195:Disease Models, Animal; D008297:Male; D011446:Prospective Studies; D062326:Re-Epithelialization; D012867:Skin / Q000293:injuries / Q000473:pathology; D034421:Sus scrofa; D013552:Swine; D014945:Wound Healing
keywords: Burns; Burns depth; Histology; Macroscopy; Porcine burn model; Wound healing