title: [Nephrotoxicity of polymyxin B: experimental study in cells and implications for nursing practice].
abstract: The aim of the study was to characterize the cell damage mechanisms involved in the pathophysiology of cytotoxicity of polymyxin B in proximal tubular cells (LLC - PK1) and discuss about the nurses interventions to identify at risk patients and consider prevention or treatment of nephrotoxicity acute kidney injury. This is a quantitative experimental in vitro study, in which the cells were exposed to 375μM polymyxin B sulfate concentration. Cell viability was determined by exclusion of fluorescent dyes and morphological method with visualization of apoptotic bodies for fluorescence microscopy. Cells exposed to polymyxin B showed reduced viability, increased number of apoptotic cells and a higher concentration of the enzyme lactate dehydrogenase. The administration of polymyxin B in vitro showed the need for actions to minimize adverse effects such as nephrotoxicity.
authors.affiliation: Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Luciana Barros de Moura
authors.initials: LB
authors.lastname: Neiva
authors.affiliation: Department of Nephrology, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Fernanda Teixeira
authors.initials: FT
authors.lastname: Borges
authors.affiliation: Department of Nephrology, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Mirian
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Escola Paulista de Enfermagem, Department of Clinical and Surgical Nursing, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Edson de Andrade
authors.initials: Ede A
authors.lastname: Pessoa
authors.affiliation: School of Nursing, Department of Medical-Surgical Nursing, Universidade Federal de São Paulo, São Paulo, SP, Brazil, nephron@usp.br.
authors.forename: Dulce Aparecida
authors.initials: DA
authors.lastname: Barbosa
authors.forename: Maria de Fatima Fernandes
authors.initials: Mde F
authors.lastname: Vattimo
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D007674:Kidney Diseases / Q000139:chemically induced* / Q000451:nursing / Q000517:prevention & control; D018374:LLC-PK1 Cells; D011112:Polymyxin B / Q000009:adverse effects*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D011112:Polymyxin B