title: Glucose-sensitive colorimetric sensor based on peroxidase mimics activity of porphyrin-Fe3O4 nanocomposites.
abstract: 5,10,15,20-Tetrakis(4-carboxyphenyl)-porphyrin-functionalized Fe3O4 nanocomposites (H2TCPP-Fe3O4) were successfully prepared by a simple two-step method. These nanocomposites exhibited ultra-high peroxidase-like activity compared with pure Fe3O4 nanoparticles. Colorless peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) was changed by H2O2 to its blue oxidized state. Kinetic studies indicated that the H2TCPP-Fe3O4 nanocomposites exhibited enhanced affinity toward H2O2 with a higher catalytic activity than Fe3O4 nanoparticles alone. Results of a fluorescent probe suggested that the catalase-mimic activity of the H2TCPP-Fe3O4 nanocomposites effectively catalyzed the decomposition of H2O2 into hydroxyl radicals. A simple, sensitive, and selective visual and colorimetric method with TMB as the substrate was designed to detect glucose when combined with glucose oxidase. This colorimetric method can be used for colorimetric detection of H2O2 with a minimum detection limit of 1.07×10(-6) M and a dynamic range of 5×10(-6) mol·L(-1) to 8×10(-5) mol·L(-1). This method can also be used to detect glucose at a minimum detection limit of 2.21×10(-6) M and a dynamic range of 25×10(-6) mol·L(-1) to 5×10(-6) mol·L(-1). Furthermore, the robustness of the nanocomposites makes them suitable for a wide range of applications in biomedicine and environmental chemistry fields.
authors.affiliation: College of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, P. R. China. Electronic address: qyliu@sdust.edu.cn.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, P. R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, P. R. China.
authors.forename: Qingru
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: College of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, P. R. China.
authors.forename: Renren
authors.initials: R
authors.lastname: Zhu
authors.affiliation: College of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, P. R. China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, P. R. China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Jia
authors.affiliation: College of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, P. R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Bian
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. Electronic address: lhzhuo@ciac.jl.cn.
authors.forename: Linhai
authors.initials: L
authors.lastname: Zhuo
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D002384:Catalysis; D003124:Colorimetry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D011166:Porphyrins / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D001786:Blood Glucose; D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D011166:Porphyrins; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D009195:Peroxidase; D052203:Ferrosoferric Oxide
keywords: Colorimetric sensor; Glucose; H(2)TCPP-Fe(3)O(4) nanocomposites; Peroxidase