title: Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture.
abstract: Circulating tumor cells (CTCs) in the blood which have detached from both the primary tumor and any metastases may be considered as a "liquid biopsy" and are expected to replace tumor biopsies in the monitoring of treatment response and determining patient prognosis. Here, we introduce a facile and efficient CTC detection material made of hydroxyapatite/chitosan (HA/CTS), which is beneficial because of its transparency and excellent biological compatibility. Atomic force microscopy images show that the roughness of the HA/CTS nanofilm (HA/CTSNF) substrates can be controlled by changing the HA:CTS ratio. Enhanced local topographic interactions between nano-components on cancer cell membranes, and the antibody coated nanostructured substrate lead to improved CTC capture and separation. This remarkable nanostructured substrate has the potential for CTC culture in situ and merits further analysis. CTCs captured from artificial blood samples were observed in culture on HA/CTSNF substrates over a period of 14 days by using conventional staining methods (hematoxylin eosin and Wright's stain). We conclude that these substrates are multifunctional materials capable of isolating and culturing CTCs for subsequent studies.
authors.affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, People's Republic of China.
authors.forename: Boran
authors.initials: B
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, Hubei, People's Republic of China.
authors.forename: Zhaobo
authors.initials: Z
authors.lastname: He
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, Hubei, People's Republic of China.
authors.forename: Libo
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, Hubei, People's Republic of China.
authors.forename: Rongxiang
authors.initials: R
authors.lastname: He
authors.affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, People's Republic of China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, People's Republic of China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Song
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, Hubei, People's Republic of China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, Hubei, People's Republic of China.
authors.forename: Xingzhong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xiong
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D012031:Refractometry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan; D017886:Durapatite
keywords: cell capture; cell culture; hydroxyapatite/chitosan; nanofilms