title: Transmission geometry laserspray ionization vacuum using an atmospheric pressure inlet.
abstract: This represents the first report of laserspray ionization vacuum (LSIV) with operation directly from atmospheric pressure for use in mass spectrometry. Two different types of electrospray ionization source inlets were converted to LSIV sources by equipping the entrance of the atmospheric pressure inlet aperture with a customized cone that is sealed with a removable glass plate holding the matrix/analyte sample. A laser aligned in transmission geometry (at 180° relative to the inlet) ablates the matrix/analyte sample deposited on the vacuum side of the glass slide. Laser ablation from vacuum requires lower inlet temperature relative to laser ablation at atmospheric pressure. However, higher inlet temperature is required for high-mass analytes, for example, α-chymotrypsinogen (25.6 kDa). Labile compounds such as gangliosides and cardiolipins are detected in the negative ion mode directly from mouse brain tissue as intact doubly deprotonated ions. Multiple charging enhances the ion mobility spectrometry separation of ions derived from complex tissue samples.
authors.affiliation: Department of Chemistry, Wayne State University , Detroit, Michigan 48202, United States.
authors.forename: Corinne A
authors.initials: CA
authors.lastname: Lutomski
authors.forename: Tarick J
authors.initials: TJ
authors.lastname: El-Baba
authors.forename: Ellen D
authors.initials: ED
authors.lastname: Inutan
authors.forename: Cory D
authors.initials: CD
authors.lastname: Manly
authors.forename: James
authors.initials: J
authors.lastname: Wager-Miller
authors.forename: Ken
authors.initials: K
authors.lastname: Mackie
authors.forename: Sarah
authors.initials: S
authors.lastname: Trimpin
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D001923:Brain Chemistry; D002308:Cardiolipins / Q000032:analysis; D004867:Equipment Design; D005732:Gangliosides / Q000032:analysis; D007834:Lasers; D013058:Mass Spectrometry / Q000295:instrumentation*; D051379:Mice; D014618:Vacuum
chemical_list: D002308:Cardiolipins; D005732:Gangliosides