title: Cytotoxic and antigenotoxic activities of phosvitin from egg yolk.
abstract: Egg yolk phosvitin is one of the most phosphorylated proteins in nature, and thus has a strong metal-binding ability. The objective of this study was to evaluate the cytotoxic and antigenotoxic activities of phosvitin in vitro. Using the 3-[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, the cytotoxicity of phosvitin was evaluated in human cancer cell lines of various tissue origins, including the cervix (HeLa), breast (MCF-7), stomach (AGS), lung (A549 and SK-MES-1), liver (HepG2), and larynx (Hep-2). The growth of all cancer cell lines was inhibited in a dose-dependent manner by phosvitin. Among the cancer cell lines tested, MCF-7 and SK-MES-1 were the least sensitive and HeLa, AGS, and HepG2 were the most sensitive to phosvitin. The 50% inhibition of cell viability values of phosvitin were 5.38, 11.57, 4.78, 6.98, 11.82, 3.93, and 9.97 mg/mL for HeLa, MCF-7, AGS, A549, SK-MES-1, HepG2, and Hep-2, respectively. The protective effects of phosvitin against DNA damage in human leukocytes indicated that phosvitin showed protective effects against the oxidative stress-induced DNA damages in human leukocytes. These results suggested that phosvitin has a high potential to be used as an anticancer agent for humans.
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Korea Department of Animal Science, Iowa State University, Ames 50011.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Korea.
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Kyungnam University, Changwon 631-701, Korea.
authors.forename: Minhee
authors.initials: M
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Kyungnam University, Changwon 631-701, Korea.
authors.forename: Eunju
authors.initials: E
authors.lastname: Park
authors.affiliation: Department of Animal Science, Iowa State University, Ames 50011 Department of Animal Science and Technology, Sunchon National University, Sunchon 540-742, Korea hdpaik@konkuk.ac.kr duahn@iastate.edu.
authors.forename: Dong Uk
authors.initials: DU
authors.lastname: Ahn
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Korea Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea hdpaik@konkuk.ac.kr duahn@iastate.edu.
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Paik
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004530:Egg Yolk / Q000737:chemistry*; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects*; D061986:MCF-7 Cells; D010774:Phosvitin / Q000737:chemistry / Q000494:pharmacology*; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D010774:Phosvitin; C022616:thiazolyl blue
keywords: DNA damage; antigenotoxic activity; cell growth; cytotoxic activity; phosvitin