title: Potential prognostic and diagnostic application of a novel monoclonal antibody against keratinocyte growth factor receptor.
abstract: KGFR is involved in the pathogenesis of several human cancers. In this study, we generated and characterized a monoclonal antibody specific to KGFR (SC-101 mAb) and evaluated its potential use in basic research and as a diagnostic and prognostic tool. The specificity and biological activity of the SC-101 mAb were evaluated by Western blotting, immunofluorescence, and immunoprecipitation analyses on various cell lines. KGFR expression in breast, pancreatic, and thyroid carcinoma was assessed by immunohistochemistry (IHC) with SC-101 mAb. KGFR expression levels revealed by SC-101 mAb resulted to increase proportionally with tumor grade in breast and pancreatic cancer. In addition, SC-101 mAb was able to detect KGFR down-modulation in thyroid cancer. SC-101 mAb might represent a useful tool for basic research applications, and it could also contribute to improve the accuracy of diagnosis and prognosis of epithelial tumors.
authors.affiliation: Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161, Rome, Italy, simona.ceccarelli@uniroma1.it.
authors.forename: Simona
authors.initials: S
authors.lastname: Ceccarelli
authors.forename: Roberto
authors.initials: R
authors.lastname: Bei
authors.forename: Enrica
authors.initials: E
authors.lastname: Vescarelli
authors.forename: Sirio
authors.initials: S
authors.lastname: D'Amici
authors.forename: Cira
authors.initials: C
authors.lastname: di Gioia
authors.forename: Andrea
authors.initials: A
authors.lastname: Modesti
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Romano
authors.forename: Adriano
authors.initials: A
authors.lastname: Redler
authors.forename: Cinzia
authors.initials: C
authors.lastname: Marchese
authors.forename: Antonio
authors.initials: A
authors.lastname: Angeloni
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity / Q000276:immunology; D045744:Cell Line, Tumor; D051523:Fibroblast Growth Factor 7 / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis* / Q000276:immunology; D011379:Prognosis; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D051523:Fibroblast Growth Factor 7; D051497:Receptor, Fibroblast Growth Factor, Type 2; C065104:keratinocyte growth factor receptor