title: Use of COI, CytB and ND5 genes for intra- and inter-specific differentiation of Haematobia irritans and Haematobia exigua.
abstract: A multilocus sequence analysis using mitochondria-encoded cytochrome c oxidase subunit I (COI), cytochrome B (CytB), NADH dehydrogenase subunit 5 (ND5); nuclear encoded 18S ribosomal RNA (18S) and 28S ribosomal RNA (28S) genes was performed to determine the levels of genetic variation between the closely related species Haematobia irritans Linnaeus and Haematobia exigua de Meijere. Among these five genes, ND5 and CytB genes were found to be more variable and informative in resolving the interspecific relationships of both species. In contrast, the COI gene was more valuable in inferring the intraspecific relationships. The ribosomal 18S and 28S sequences of H. irritans and H. exigua were highly conserved with limited intra- and inter-specific variation. Molecular evidence presented in this study demonstrated that both flies are genetically distinct and could be differentiated based on sequence analysis of mitochondrial genes.
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: lucaslow24@gmail.com.
authors.forename: Van Lun
authors.initials: VL
authors.lastname: Low
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Tiong Kai
authors.initials: TK
authors.lastname: Tan
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Phaik Eem
authors.initials: PE
authors.lastname: Lim
authors.affiliation: Louisiana State University Agricultural Center, Department of Entomology, 404 Life Science Building, Baton Rouge, LA 70803, United States.
authors.forename: Luísa Nogueira
authors.initials: LN
authors.lastname: Domingues
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Sun Tee
authors.initials: ST
authors.lastname: Tay
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yvonne Ai Lian
authors.initials: YA
authors.lastname: Lim
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Thary Gazi
authors.initials: TG
authors.lastname: Goh
authors.affiliation: Veterinary Research Institute, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia.
authors.forename: Chandrawathani
authors.initials: C
authors.lastname: Panchadcharam
authors.affiliation: Veterinary Research Institute, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia.
authors.forename: Premaalatha
authors.initials: P
authors.lastname: Bathmanaban
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mohd
authors.initials: M
authors.lastname: Sofian-Azirun
mesh_terms: D000818:Animals; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing / Q000662:veterinary; D009117:Muscidae / Q000201:enzymology* / Q000235:genetics; D009245:NADH Dehydrogenase / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D045303:Cytochromes b; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV
keywords: Buffalo fly; COI; CytB; Horn fly; Molecular markers; ND5