title: Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
abstract: Dendritic cells (DCs) play a critical role in immune homeostasis by regulating the functions of various immune cells, including T and B cells. Notably, DCs also undergo education on reciprocal signalling by these immune cells and environmental factors. Various reports demonstrated that B cells have profound regulatory functions, although only few reports have explored the regulation of human DCs by B cells. Here we demonstrate that activated but not resting B cells induce maturation of DCs with distinct features to polarize Th2 cells that secrete interleukin (IL)-5, IL-4 and IL-13. B-cell-induced maturation of DCs is contact dependent and implicates signalling of B-cell activation molecules CD69, B-cell-activating factor receptor, and transmembrane activator and calcium-modulating cyclophilin ligand interactor. Mechanistically, differentiation of Th2 cells by B-cell-matured DCs is dependent on OX-40 ligand. Collectively, our results suggest that B cells have the ability to control their own effector functions by enhancing the ability of human DCs to mediate Th2 differentiation.
authors.affiliation: 1] Institut National de la Santé et de la Recherche Médicale Unité 1138, Paris F-75006, France [2] Centre de Recherche des Cordeliers, Equipe 16- Immunopathology and Therapeutic Immunointervention, Université Pierre et Marie Curie - Paris 6, UMR S 1138, 15 rue de l'Ecole de Médicine, Paris F-75006, France [3] Université Paris Descartes, UMR S 1138, Paris F-75006, France [4] Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, 954 Gatewood Road, Atlanta, Georgia 30329, USA.
authors.forename: Mohan S
authors.initials: MS
authors.lastname: Maddur
authors.affiliation: 1] Institut National de la Santé et de la Recherche Médicale Unité 1138, Paris F-75006, France [2] Université de Technologie de Compiègne, Compiègne F-60205, France.
authors.forename: Meenu
authors.initials: M
authors.lastname: Sharma
authors.affiliation: 1] Institut National de la Santé et de la Recherche Médicale Unité 1138, Paris F-75006, France [2] Université de Technologie de Compiègne, Compiègne F-60205, France.
authors.forename: Pushpa
authors.initials: P
authors.lastname: Hegde
authors.affiliation: 1] Institut National de la Santé et de la Recherche Médicale Unité 1138, Paris F-75006, France [2] Centre de Recherche des Cordeliers, Equipe 16- Immunopathology and Therapeutic Immunointervention, Université Pierre et Marie Curie - Paris 6, UMR S 1138, 15 rue de l'Ecole de Médicine, Paris F-75006, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Stephen-Victor
authors.affiliation: Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, 954 Gatewood Road, Atlanta, Georgia 30329, USA.
authors.forename: Bali
authors.initials: B
authors.lastname: Pulendran
authors.affiliation: 1] Institut National de la Santé et de la Recherche Médicale Unité 1138, Paris F-75006, France [2] Centre de Recherche des Cordeliers, Equipe 16- Immunopathology and Therapeutic Immunointervention, Université Pierre et Marie Curie - Paris 6, UMR S 1138, 15 rue de l'Ecole de Médicine, Paris F-75006, France [3] Université Paris Descartes, UMR S 1138, Paris F-75006, France [4] International Associated Laboratory IMPACT (Institut National de la Santé et de la Recherche Médicale, France - Indian Council of Medical Research, India), National Institute of Immunohaematology, Mumbai 400012, India.
authors.forename: Srini V
authors.initials: SV
authors.lastname: Kaveri
authors.affiliation: 1] Institut National de la Santé et de la Recherche Médicale Unité 1138, Paris F-75006, France [2] Centre de Recherche des Cordeliers, Equipe 16- Immunopathology and Therapeutic Immunointervention, Université Pierre et Marie Curie - Paris 6, UMR S 1138, 15 rue de l'Ecole de Médicine, Paris F-75006, France [3] Université Paris Descartes, UMR S 1138, Paris F-75006, France [4] International Associated Laboratory IMPACT (Institut National de la Santé et de la Recherche Médicale, France - Indian Council of Medical Research, India), National Institute of Immunohaematology, Mumbai 400012, India.
authors.forename: Jagadeesh
authors.initials: J
authors.lastname: Bayry
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D053265:B-Cell Activation Factor Receptor / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D037181:Lectins, C-Type / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D053278:OX40 Ligand / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D053303:Transmembrane Activator and CAML Interactor Protein / Q000276:immunology; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D053265:B-Cell Activation Factor Receptor; D018793:Interleukin-13; D015847:Interleukin-4; D015848:Interleukin-5; D037181:Lectins, C-Type; D053278:OX40 Ligand; D053303:Transmembrane Activator and CAML Interactor Protein; D000099192:CD69 Antigens; C508598:IL4 protein, human; C508599:IL5 protein, human; C108467:TNFRSF13B protein, human; C435926:TNFRSF13C protein, human; C089251:TNFSF4 protein, human