title: D-galactose induced inflammation lipid peroxidation and platelet activation in rats.
abstract: BACKGROUND
  To investigate events possibly related to the development of D-galactose induced senescence, we examined whether 8-iso PGF(2α) formation, a marker of in vivo lipid peroxidation is altered and whether its biosynthesis is associated with 11-dehydro-TXB(2) excretion rate, as a marker of in vivo platelet activation. In this setting, we also investigated the relationship between proinflammatory mediators (IL-6 and TNF-α from one, and lipid peroxidation and platelet activation, from another aspect.
  
  
  RESULTS
  Forty animals were divided, depending on treatment with d-galactose into: placebo and D-galactose treated rats. 8-iso-PGF(2α), IL-6 and TNF-α were measured in plasma, while 11-dehydro-TXB(2) was determined in the urine after a six week treatment with d-galactose. Compared to placebo, d-galactose treated animals showed significantly higher levels of all measured parameters.
  
  
  CONCLUSIONS
  D-galactose induced changes in the rate of F(2)-isoprostane formation are associated with the changes in the excretion rate of 11-dehydro-TXB(2).
authors.affiliation: Faculty of Natural Sciences and Mathematics, Institute of Biology, "Ss. Cyril and Methodius" University, P.O. Box 162, 1000 Skopje, Macedonia.
authors.forename: Nikola
authors.initials: N
authors.lastname: Hadzi-Petrushev
authors.affiliation: Faculty of Veterinary Medicine, "Ss. Cyril and Methodius" University, 1000 Skopje, Macedonia.
authors.forename: Velimir
authors.initials: V
authors.lastname: Stojkovski
authors.affiliation: Faculty of Veterinary Medicine, "Ss. Cyril and Methodius" University, 1000 Skopje, Macedonia.
authors.forename: Dine
authors.initials: D
authors.lastname: Mitrov
authors.affiliation: Faculty of Natural Sciences and Mathematics, Institute of Biology, "Ss. Cyril and Methodius" University, P.O. Box 162, 1000 Skopje, Macedonia. Electronic address: mitkom@pmf.ukim.mk.
authors.forename: Mitko
authors.initials: M
authors.lastname: Mladenov
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D016922:Cellular Senescence; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000097:blood; D005690:Galactose / Q000494:pharmacology*; D007249:Inflammation / Q000139:chemically induced; D015850:Interleukin-6 / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D015539:Platelet Activation / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D013929:Thromboxane B2 / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C075750:8-epi-prostaglandin F2alpha; D016718:Arachidonic Acid; D013929:Thromboxane B2; C049235:11-dehydro-thromboxane B2; D015237:Dinoprost; D005690:Galactose
keywords: Inflammation; Lipid peroxidation; Platelet activation; Rats; d-galactose induced senescence