title: Discovery of a new antiviral protein isolated Lonomia obliqua analysed by bioinformatics and real-time approaches.
abstract: This study presents a new recombinant protein that acts as a powerful antiviral (rAVLO-recombinant Antiviral protein of Lonomia obliqua). It was able to reduce the replication by 10(6) fold for herpes virus and by 10(4) fold for rubella virus. RT-PCR of viral RNA rAVLO treated infected cells also showed similar rate of inhibition in replication. The analysis of this protein by bioinformatics suggests that this protein is globular, secreted with a signal peptide and has the ability to bind to MHC class I. It was found that there are several protein binding sites with various HLA and a prevalence of α-helices in the N-terminal region (overall classified as a α/β protein type). BLAST similarity sequence search for corresponding cDNA did not reveal a similar sequence in Genbank, suggesting that it is from a novel protein family. In this study we have observed that this recombinant protein and hemolymph has a potent antiviral action. This protein was produced in a baculovirus/Sf-9 system. Therefore, these analyses suggest that this novel polypeptide is a candidate as a broad spectrum antiviral.
authors.affiliation: Laboratório de Parasitologia, Instituto Butantan, São Paulo, Cep. 05503-000, Brazil. viegasacc@gmail.com.
authors.forename: Ana Carolina Viegas
authors.initials: AC
authors.lastname: Carmo
authors.affiliation: Laboratório de Estudos Genômicos, IBILCE-UNESP, São Paulo, Brazil.
authors.forename: Lilian Hiromi Tomanari
authors.initials: LH
authors.lastname: Yamasaki
authors.affiliation: Núcleo de Doenças Respiratórias, Centro de Virologia, Instituto Adolfo Lutz, São Paulo, Brazil.
authors.forename: Cristina Adelaide
authors.initials: CA
authors.lastname: Figueiredo
authors.affiliation: Laboratório de Parasitologia, Instituto Butantan, São Paulo, Cep. 05503-000, Brazil.|Laboratório de Estudos Genômicos, IBILCE-UNESP, São Paulo, Brazil.|Núcleo de Doenças Respiratórias, Centro de Virologia, Instituto Adolfo Lutz, São Paulo, Brazil.|Núcleo de Doenças de Transmissão Vetorial, Centro de Virologia, Instituto Adolfo Lutz, São Paulo, Brazil.
authors.forename: Dalton Nogueira
authors.initials: DN
authors.lastname: da Silva Giovanni
authors.affiliation: Núcleo de Doenças Respiratórias, Centro de Virologia, Instituto Adolfo Lutz, São Paulo, Brazil.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: de Oliveira
authors.affiliation: Núcleo de Doenças Respiratórias, Centro de Virologia, Instituto Adolfo Lutz, São Paulo, Brazil.
authors.forename: Fabiana Cristina Pereira
authors.initials: FC
authors.lastname: Dos Santos
authors.affiliation: Núcleo de Doenças de Transmissão Vetorial, Centro de Virologia, Instituto Adolfo Lutz, São Paulo, Brazil.
authors.forename: Suely Pires
authors.initials: SP
authors.lastname: Curti
authors.affiliation: Laboratório de Estudos Genômicos, IBILCE-UNESP, São Paulo, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Rahal
authors.affiliation: Laboratório de Parasitologia, Instituto Butantan, São Paulo, Cep. 05503-000, Brazil.
authors.forename: Ronaldo Zucatelli
authors.initials: RZ
authors.lastname: Mendonça
keywords: Antiviral; Bioinformatics; Lonomia obliqua