title: Does insulin, transferrin and selenous acid preparation effect chondrocyte proliferation?
abstract: OBJECTIVE
  The aim of this study was to test the hypothesis that insulin, human transferrin, and selenous acid (ITS) preparation have positive effects on chondrocyte proliferation and morphology and investigate the biochemical and histological effects of these additive substances in different cell culture media.
  
  
  METHODS
  Human cartilage-derived cells (hCDCs) were isolated from the cartilage tissue of a 57-year-old woman diagnosed with gonarthrosis. Tissue samples were cultured in Dulbecco's modified Eagle's medium (DMEM) and RPMI-1640. The cells' chondrogenic activities were observed. After serial passagings, cells were divided into 4 groups at the end of the 6th week. On the 14th day, proliferated cells were examined using an inverted microscope with x4, x10, x20 and x40 magnification and microphotographs were taken. Living cell quantity was determined on the first and 14th days using MTS-ELISA cell proliferation assay.
  
  
  RESULTS
  DMEM (without adding ITS premix solution) and RPMI-1640 containing ITS premix solution provide proliferation of the chondrogenic cells. The proliferation and viability of chondrocytes were revealed in this study in the 3rd group (DMEM solution without additives).
  
  
  CONCLUSIONS
  It is suggested that the culture medium ingredients play crucial roles on chondrogenic proliferation in osteochondral tissue cultures.
authors.affiliation: Nişantaşı University, İstanbul, Turkey. dralpergokce@yahoo.com.
authors.forename: Alper
authors.initials: A
authors.lastname: Gökçe
authors.affiliation: Rational Drug Use Team, Tekirdağ State Hospital, Tekirdağ, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Yılmaz
authors.affiliation: Esencan Hospital, İstanbul Esenyurt University, İstanbul, Turkey.
authors.forename: Nevzat Selim
authors.initials: NS
authors.lastname: Gökay
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Namık Kemal University, Tekirdağ, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Can
authors.affiliation: Üsküdar Directorate of Health, İstanbul, Turkey.
authors.forename: Ciğdem
authors.initials: C
authors.lastname: Gökçe
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects*; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D020887:Selenious Acid / Q000494:pharmacology*; D014168:Transferrin / Q000494:pharmacology*
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; D014168:Transferrin; D020887:Selenious Acid