title: Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings.
abstract: OBJECTIVE
  To develop and test a protocol for isolation of potential auto-antigens from chorionic tissue that may be linked to recurrent miscarriage (RM).
  
  
  METHODS
  The strategy included: 1) isolation of IgGs tightly bound to chorionic tissue of RM patients by protein G chromatography; 2) construction of affinity columns using the chorionic antibodies for isolation of auto-antigens; 3) enrichment of auto-antigens from detergent extracted solution of chorionic proteins by affinity chromatography; 4) separation by dodecyl sulfate-electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry identification.
  
  
  RESULTS
  Five potential auto-antigens were detected: neutral alpha-glucosidase AB, endoplasmin, transitional endoplasmic reticulum ATPase, putative endoplasmin-like protein, and cytoplasmic actin 2.
  
  
  CONCLUSIONS
  We developed a strategy for identification of auto-antigens in the chorionic tissue of women with RM, which could be of diagnostic and prognostic value.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Kit
authors.forename: Marina
authors.initials: M
authors.lastname: Starykovych
authors.forename: Marie
authors.initials: M
authors.lastname: Vajrychova
authors.forename: Juraj
authors.initials: J
authors.lastname: Lenco
authors.forename: Danuta
authors.initials: D
authors.lastname: Zastavna
authors.affiliation: Rostyslav Stoika, Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Str. 14/16, 79005, Lviv, Ukraine, stoika@cellbiol.lviv.ua.
authors.forename: Rostyslav
authors.initials: R
authors.lastname: Stoika
mesh_terms: D000026:Abortion, Habitual / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000328:Adult; D001323:Autoantibodies / Q000032:analysis; D001324:Autoantigens / Q000378:metabolism*; D015153:Blotting, Western; D002823:Chorion / Q000378:metabolism*; D002846:Chromatography, Affinity; D004527:Egg Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005260:Female; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D055815:Young Adult; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000199:Actins; D001323:Autoantibodies; D001324:Autoantigens; D004527:Egg Proteins; D008562:Membrane Glycoproteins; C015753:chorion proteins; C053387:endoplasmin; D000520:alpha-Glucosidases; D000251:Adenosine Triphosphatases