title: Intramolecular C(sp(3))H amination of arylsulfonyl azides with engineered and artificial myoglobin-based catalysts.
abstract: The direct conversion of aliphatic CH bonds into CN bonds provides an attractive approach to the introduction of nitrogen-containing functionalities in organic molecules. Following the recent discovery that cytochrome P450 enzymes can catalyze the cyclization of arylsulfonyl azide compounds via an intramolecular C(sp(3))H amination reaction, we have explored here the CH amination reactivity of other hemoproteins. Various heme-containing proteins, and in particular myoglobin and horseradish peroxidase, were found to be capable of catalyzing this transformation. Based on this finding, a series of engineered and artificial myoglobin variants containing active site mutations and non-native Mn- and Co-protoporphyrin IX cofactors, respectively, were prepared to investigate the effect of these structural changes on the catalytic activity and selectivity of these catalysts. Our studies showed that metallo-substituted myoglobins constitute viable CH amination catalysts, revealing a distinctive reactivity trend as compared to synthetic metalloporphyrin counterparts. On the other hand, amino acid substitutions at the level of the heme pocket were found to be beneficial toward improving the stereo- and enantioselectivity of these Mb-catalyzed reactions. Mechanistic studies involving kinetic isotope effect experiments indicate that CH bond cleavage is implicated in the rate-limiting step of myoglobin-catalyzed amination of arylsulfonyl azides. Altogether, these studies indicate that myoglobin constitutes a promising scaffold for the design and development of CH amination catalysts.
authors.affiliation: Department of Chemistry, University of Rochester, Rochester, NY 14627, United States.
authors.forename: Melanie
authors.initials: M
authors.lastname: Bordeaux
authors.affiliation: Department of Chemistry, University of Rochester, Rochester, NY 14627, United States.
authors.forename: Ritesh
authors.initials: R
authors.lastname: Singh
authors.affiliation: Department of Chemistry, University of Rochester, Rochester, NY 14627, United States. Electronic address: fasan@chem.rochester.edu.
authors.forename: Rudi
authors.initials: R
authors.lastname: Fasan
mesh_terms: D000586:Amination; D000818:Animals; D001386:Azides / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D008958:Models, Molecular; D015394:Molecular Structure; D009211:Myoglobin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism*; D015202:Protein Engineering; D050661:Sperm Whale; D013237:Stereoisomerism
chemical_list: D001386:Azides; D009211:Myoglobin; D009942:Organometallic Compounds
keywords: Artificial metalloenzymes; CH amination; Myoglobin; Protein engineering; Sulfonyl azides