title: Establishment of recipient model for spermatogonial stem cells transplantation in Kunming mice.
abstract: The objective of this study was to establish a recipient model for spermatogonial stem cells (SSCs) transplantation in the Kunming mice after different doses busulfan treatment. The results showed that the most optimal dose of busulfan was 20mg/kg and the most appropriate time for transplantation was 5-7 wk after busulfan treatment. Then, the cloned fragments existed in the testis of recipient mice after 20mg/kg busulfan treatment and the offspring with enhanced green fluorescent protein (EGFP) were produced by the transplanting SSCs. Hence, we established the effective recipient model for donor-derived SSCs transplantation in Kunming mice.
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Juhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Xiuheng
authors.initials: X
authors.lastname: Xue
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Fan
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, Hefei, Anhui 230036, China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Pu
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Hongguo
authors.initials: H
authors.lastname: Cao
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, Hefei, Anhui 230036, China. Electronic address: wjhxxh@163.com.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002066:Busulfan / Q000008:administration & dosage*; D049452:Green Fluorescent Proteins / Q000737:chemistry; D008297:Male; D051379:Mice; D013091:Spermatogenesis / Q000187:drug effects; D013093:Spermatogonia / Q000166:cytology / Q000187:drug effects*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D002066:Busulfan
keywords: Busulfan; Kunming mice; Spermatogonial stem cells; Testis; Transplantation