title: Phenol content, antioxidant capacity and antibacterial activity of methanolic extracts derived from four Jordanian medicinal plants.
abstract: This study was performed to assess the antioxidant and antibacterial properties of methanolic extracts derived from aerial parts of four Jordanian medicinal plants (Artemisia sieberi, Peganum harmala, Rosmarinus officinalis (Green-Flowered) and Sarcopterium spinosium). The possible relationship between these biological properties and the total phenolic concentrations of these extracts were also be determined. The antioxidant capacity and total phenolic concentrations were assessed by the ABTS method and Folin-Ciocalteu method, respectively. The amount of the extract required to scavenge 50% of ABTS (IC50) was also measured. Broth dilution and disc diffusion assays were performed to measure the antibacterial activity of these extracts against available bacterial strains. Variations were observed among the examined plants in antioxidant and antibacterial activities as well as in their phenol contents. According to ABTS assay and IC50 value, the highest free radical scavenging potential was found in Sarcopterium spinosium, followed by Rosmarinus officinalis, Peganum harmala and Artemisia sieberi, respectively. Similarly, the results of antibacterial assays showed that Sarcopterium spinosium exhibited the highest antibacterial activity against all tested bacterial strains as compared to Rosmarinus officinalis, Peganum harmala and Artemisia sieberi. Moreover, Sarcopterium spinosium contained the highest amount of phenolic compounds followed by, Rosmarinus officinalis, Artemisia sieberi and Peganum harmala, respectively. In conclusion, these plants are not only interesting sources for antimicrobial agents but also have a considerable amount of antioxidants. In addition, these findings revealed that the antioxidant capacity and antibacterial activity of these plant extracts do not necessary be attributed to their total phenolic concentrations.
authors.forename: Fawzi I
authors.initials: FI
authors.lastname: Irshaid
authors.forename: Khalid A
authors.initials: KA
authors.lastname: Tarawneh
authors.forename: Jacob H
authors.initials: JH
authors.lastname: Jacob
authors.forename: Aisha M
authors.initials: AM
authors.lastname: Alshdefat
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D007597:Jordan; D019684:Magnoliopsida / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction / Q000187:drug effects; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D000432:Methanol