title: Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection.
abstract: OBJECTIVE
  To investigate whether tissue samples processed by the rapid urease test (RUT) kit are suitable for dual-priming oligonucleotide-based multiplex polymerase chain reaction (DPO-PCR) to detect Helicobacter pylori (H. pylori).
  
  
  METHODS
  A total of 54 patients with specific gastrointestinal symptom were enrolled in this study. During endoscopy, gastric biopsy specimens were taken for histology, RUT, and DPO-PCR. DPO-PCR was performed on gastric biopsy samples and tissue samples that were analyzed by RUT at 2 separate institutes. In detecting H. pylori, the concordance rate of the DPO-PCR tests between the tissue samples that had been submitted to RUT and the gastric biopsy samples was investigated.
  
  
  RESULTS
  H. pylori co-occurred with 76.0% (19/25) of gastric ulcers, 64.3% (9/14) of duodenal ulcers, and 33.3% (4/12) of gastritis cases. H. pylori infection was found in 100% (3/3) of the patients with both gastric and duodenal ulcers. Overall, H. pylori was detected in 35 of 54 (64.8%) patients. The diagnostic sensitivities of histology, RUT, and DPO-PCR were 85.7% (30/35), 74.3% (26/35), and 97.1% (34/35), respectively (P = 0.02). The positive predictive value (PPV) of DPO-PCR was 94.4%, whereas the negative predictive value (NPV) was 94.7%. In the rapid urease test (CLOtest)-negative cases, the frequency of positive DPO-PCR and histologic results was 20.0% (7/35). The concordance rate of the DPO-PCR tests between the tissue samples from the RUT kit and the gastric biopsy samples was 94.4% (51/54). The rate of DPO-PCR and silver stain positivity in the RUT-negative cases was 20.0% (7/35).
  
  
  CONCLUSIONS
  In diagnosing H. pylori infection, DPO-PCR can be performed on tissue samples that have been processed by the RUT kit. Particularly, in patients with RUT-negative results, DPO-PCR on these tissue samples could be helpful in detecting of H. pylori infection.
authors.affiliation: Woo Chul Chung, Sung Hoon Jung, Jung Hwan Oh, Tae Ho Kim, Dae Young Cheung, Byung Wook Kim, Sung Soo Kim, Jin Il Kim, Department of Internal Medicine, the Catholic University of Korea, Seoul 130-709, South Korea.
authors.forename: Woo Chul
authors.initials: WC
authors.lastname: Chung
authors.affiliation: Woo Chul Chung, Sung Hoon Jung, Jung Hwan Oh, Tae Ho Kim, Dae Young Cheung, Byung Wook Kim, Sung Soo Kim, Jin Il Kim, Department of Internal Medicine, the Catholic University of Korea, Seoul 130-709, South Korea.
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Jung
authors.affiliation: Woo Chul Chung, Sung Hoon Jung, Jung Hwan Oh, Tae Ho Kim, Dae Young Cheung, Byung Wook Kim, Sung Soo Kim, Jin Il Kim, Department of Internal Medicine, the Catholic University of Korea, Seoul 130-709, South Korea.
authors.forename: Jung Hwan
authors.initials: JH
authors.lastname: Oh
authors.affiliation: Woo Chul Chung, Sung Hoon Jung, Jung Hwan Oh, Tae Ho Kim, Dae Young Cheung, Byung Wook Kim, Sung Soo Kim, Jin Il Kim, Department of Internal Medicine, the Catholic University of Korea, Seoul 130-709, South Korea.
authors.forename: Tae Ho
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Woo Chul Chung, Sung Hoon Jung, Jung Hwan Oh, Tae Ho Kim, Dae Young Cheung, Byung Wook Kim, Sung Soo Kim, Jin Il Kim, Department of Internal Medicine, the Catholic University of Korea, Seoul 130-709, South Korea.
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Cheung
authors.affiliation: Woo Chul Chung, Sung Hoon Jung, Jung Hwan Oh, Tae Ho Kim, Dae Young Cheung, Byung Wook Kim, Sung Soo Kim, Jin Il Kim, Department of Internal Medicine, the Catholic University of Korea, Seoul 130-709, South Korea.
authors.forename: Byung Wook
authors.initials: BW
authors.lastname: Kim
authors.affiliation: Woo Chul Chung, Sung Hoon Jung, Jung Hwan Oh, Tae Ho Kim, Dae Young Cheung, Byung Wook Kim, Sung Soo Kim, Jin Il Kim, Department of Internal Medicine, the Catholic University of Korea, Seoul 130-709, South Korea.
authors.forename: Sung Soo
authors.initials: SS
authors.lastname: Kim
authors.affiliation: Woo Chul Chung, Sung Hoon Jung, Jung Hwan Oh, Tae Ho Kim, Dae Young Cheung, Byung Wook Kim, Sung Soo Kim, Jin Il Kim, Department of Internal Medicine, the Catholic University of Korea, Seoul 130-709, South Korea.
authors.forename: Jin Il
authors.initials: JI
authors.lastname: Kim
authors.affiliation: Woo Chul Chung, Sung Hoon Jung, Jung Hwan Oh, Tae Ho Kim, Dae Young Cheung, Byung Wook Kim, Sung Soo Kim, Jin Il Kim, Department of Internal Medicine, the Catholic University of Korea, Seoul 130-709, South Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Sin
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D017931:DNA Primers / Q000737:chemistry; D004381:Duodenal Ulcer / Q000150:complications / Q000382:microbiology; D004724:Endoscopy; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D013276:Stomach Ulcer / Q000150:complications / Q000382:microbiology; D014510:Urease / Q000032:analysis*
chemical_list: D017931:DNA Primers; D014510:Urease
keywords: Diagnosis; Dual-priming oligonucleotide-based multiplex polymerase chain reaction; Helicobacter pylori