title: Hepatoprotective effects of secoiridoid-rich extracts from Gentiana cruciata L. against carbon tetrachloride induced liver damage in rats.
abstract: The objective of this work was to investigate the effects of the methanol extracts of Gentiana cruciata L. aerial parts (GCA) and roots (GCR) against carbon tetrachloride-induced liver injury in rats. Pretreatment with GCA and GCR, containing sweroside, swertiamarin and gentiopicrin in high concentrations, dose-dependently and significantly decreased the levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase in the level of total protein was found compared with the CCl4-treated group. Moreover, oral administration of extracts significantly enhanced antioxidant enzyme activities (superoxide dismutase and catalase), increased the content of glutathione and decreased the content of TBARS. Microscopic evaluations of the liver revealed CCl4-induced lesions and related toxic manifestations that were minimal in the liver of rats pretreated with extracts at the dose of 400 mg per kg b.w. The results suggest that the use of G. cruciata extracts has a merit as a potent candidate in protecting the liver against chemical induced toxicity.
authors.affiliation: Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia. vladam@kg.ac.rs.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Mihailović
authors.forename: Jelena
authors.initials: J
authors.lastname: Katanić
authors.forename: Danijela
authors.initials: D
authors.lastname: Mišić
authors.forename: Vesna
authors.initials: V
authors.lastname: Stanković
authors.forename: Mirjana
authors.initials: M
authors.lastname: Mihailović
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Uskoković
authors.forename: Jelena
authors.initials: J
authors.lastname: Arambašić
authors.forename: Slavica
authors.initials: S
authors.lastname: Solujić
authors.forename: Milan
authors.initials: M
authors.lastname: Mladenović
authors.forename: Nevena
authors.initials: N
authors.lastname: Stanković
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D030012:Gentiana / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D057888:Iridoid Glucosides / Q000494:pharmacology; D039823:Iridoids / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011753:Pyrones / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D057888:Iridoid Glucosides; D039823:Iridoids; D010936:Plant Extracts; D011753:Pyrones; D017392:Thiobarbituric Acid Reactive Substances; C012997:gentiopicroside; C013270:swertiamarin; D002251:Carbon Tetrachloride; D002374:Catalase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D005978:Glutathione; C049412:sweroside; D001663:Bilirubin