title: Exploring the anti-apoptotic role of HAX-1 versus BCL-XL in cytokine-dependent bone marrow-derived cells from mice.
abstract: HS-1-associated protein X-1 (HAX-1) is a multi-functional protein that has been implicated in the regulation of apoptosis, cell motility and calcium homeostasis. In the present study, we set out to assess the postulated functional resemblance of HAX-1 to the BCL-2 family of anti-apoptotic proteins using non-transformed, cytokine-dependent murine bone marrow cells as a model system. BCL-X(L), but not HAX-1 protected against cytokine withdrawal-induced apoptosis while HAX-1 and BCL-X(L) significantly reduced thapsigargin-triggered (calcium-dependent) apoptosis. The data argue in favor of cell type- and stimulus-specific roles of HAX-1 in regulation of cell survival.
authors.affiliation: Department of Molecular and Translational Oncology, Cancer Center Institute, Warsaw, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Trebinska
authors.affiliation: Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Kari
authors.initials: K
authors.lastname: Högstrand
authors.affiliation: Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Alf
authors.initials: A
authors.lastname: Grandien
authors.affiliation: Department of Molecular and Translational Oncology, Cancer Center Institute, Warsaw, Poland. Electronic address: ewag@coi.waw.pl.
authors.forename: Ewa A
authors.initials: EA
authors.lastname: Grzybowska
authors.affiliation: Division of Molecular Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden. Electronic address: bengt.fadeel@ki.se.
authors.forename: Bengt
authors.initials: B
authors.lastname: Fadeel
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011506:Proteins / Q000378:metabolism*; D019284:Thapsigargin / Q000494:pharmacology; D051020:bcl-X Protein / Q000378:metabolism*
chemical_list: D016207:Cytokines; C491295:Hs1bp1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D051020:bcl-X Protein; D019284:Thapsigargin; D002118:Calcium
keywords: Apoptosis; BCL-X(L); BH domain; HS-1-associated protein X-1; Mitochondria; Thapsigargin