title: Acupuncture at HT7 suppresses morphine self-administration at high dose through GABA system.
abstract: In the previous study, acupuncture at HT7 has shown to attenuate the self-administration of morphine at a low dose (0.1mg/kg). In this study, it was further investigated whether acupuncture at HT7 could attenuate the morphine self-administration at a high dose (0.5mg/kg). Male Sprague-Dawley rats weighing 270-300g were used. After surgery of catheterization, animals were trained to self-administer morphine solution (0.5mg/kg) using daily 1h session under fixed ratio 1 schedule for 3 weeks. Animals that had shown stable morphine-taking (establish baseline: variation less than 20% of the mean of three consecutive days) were subjected to the acupuncture treatment. Bicuculline and SCH 50911 were used to investigate the possible relation between the effect of acupuncture and the GABA receptor system. Acupuncture at HT7, but not at control acupoint, LI5, suppressed spontaneous morphine-taking behavior significantly. In addition, the effect of acupuncture was blocked by both GABA receptor antagonists. The results of this study suggest that acupuncture at HT7 suppresses morphine-taking behavior through the mediation of GABA receptor system.
authors.affiliation: Department of Acupuncture, Moxibustion, and Acupoint, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea. Electronic address: dlqhdgy@dhu.ac.kr.
authors.forename: Bong Hyo
authors.initials: BH
authors.lastname: Lee
authors.affiliation: Department of Acupuncture, Moxibustion, and Acupoint, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Ku
authors.affiliation: Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, China.
authors.forename: Rong Jie
authors.initials: RJ
authors.lastname: Zhao
authors.affiliation: Department of Physiology, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea.
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Physiology, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea.
authors.forename: Chae Ha
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Department of Physiology, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea.
authors.forename: Young S
authors.initials: YS
authors.lastname: Gwak
authors.affiliation: Department of Physiology, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea.
authors.forename: Su Chan
authors.initials: SC
authors.lastname: Chang
authors.affiliation: Department of Acupuncture, Moxibustion, and Acupoint, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea.
authors.forename: Nam Jun
authors.initials: NJ
authors.lastname: Kim
authors.affiliation: Department of Acupuncture, Moxibustion, and Acupoint, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea.
authors.forename: Jae Su
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Acupuncture, Moxibustion, and Acupoint, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea.
authors.forename: Yun Kyu
authors.initials: YK
authors.lastname: Lee
authors.affiliation: Department of Acupuncture, Moxibustion, and Acupoint, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea.
authors.forename: Hyun Jong
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Acupuncture, Moxibustion, and Acupoint, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea. Electronic address: now123@dreamwiz.com.
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Lim
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D058788:GABA-B Receptor Antagonists / Q000494:pharmacology; D008297:Male; D009020:Morphine / Q000008:administration & dosage*; D009021:Morphine Dependence / Q000378:metabolism / Q000517:prevention & control* / Q000523:psychology; D009025:Morpholines / Q000494:pharmacology; D009043:Motor Activity; D009294:Narcotics / Q000008:administration & dosage*; D009474:Neurons / Q000502:physiology; D017207:Rats, Sprague-Dawley; D018079:Receptors, GABA / Q000502:physiology*; D012646:Self Administration
chemical_list: C095804:(+)-(S)-5,5-dimethylmorpholinyl-2-acetic acid; D058787:GABA-A Receptor Antagonists; D058788:GABA-B Receptor Antagonists; D009025:Morpholines; D009294:Narcotics; D018079:Receptors, GABA; D009020:Morphine; D001640:Bicuculline
keywords: Acupuncture; GABA; HT7; High dose; Morphine; Self-administration