title: Naringenin (NAR) and 8-prenylnaringenin (8-PN) reduce the developmental competence of porcine oocytes in vitro.
abstract: Flavanones such as naringenin (NAR) and 8-prenylnaringenin (8-PN) are increasingly used as dietary supplements despite scientific concern regarding adverse effects on female reproduction upon excessive intake. In the present study, NAR and 8-PN (0.3-1μM) significantly affected porcine oocyte maturation in vitro by decreasing cumulus expansion. In addition, NAR and 8-PN decreased percentages of meiotic spindle formation, oocyte cleavage and blastocyst formation. The effects of NAR and 8-PN were different from estradiol (3.12μM)-induced effects. Still, the flavanone-induced effects were observed at concentrations that can be found in human plasma upon supplement intake and that resemble physiological estrogen equivalence levels in follicular fluids. Considering that abnormal oocyte maturation can cause subfertility, our study warrants that precautions are in place and excessive intake of NAR and 8-PN e.g. via dietary supplements should be avoided by women.
authors.affiliation: Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine Utrecht University, Yalelaan 104, 3584 CM, Utrecht, The Netherlands. Electronic address: K.A.Solak@uu.nl.
authors.forename: Kamila A
authors.initials: KA
authors.lastname: Solak
authors.affiliation: Division of Veterinary Pharmacology, Pharmacy and Toxicology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584 CM Utrecht, The Netherlands.
authors.forename: Regiane R
authors.initials: RR
authors.lastname: Santos
authors.affiliation: Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine Utrecht University, Yalelaan 104, 3584 CM, Utrecht, The Netherlands.
authors.forename: Martin
authors.initials: M
authors.lastname: van den Berg
authors.affiliation: Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine Utrecht University, Yalelaan 104, 3584 CM, Utrecht, The Netherlands.
authors.forename: Bas J
authors.initials: BJ
authors.lastname: Blaauboer
authors.affiliation: Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584 CM Utrecht, The Netherlands; Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584 CM Utrecht, The Netherlands.
authors.forename: Bernard A J
authors.initials: BA
authors.lastname: Roelen
authors.affiliation: Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine Utrecht University, Yalelaan 104, 3584 CM, Utrecht, The Netherlands.
authors.forename: Majorie B M
authors.initials: MB
authors.lastname: van Duursen
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D019587:Dietary Supplements / Q000633:toxicity*; D005260:Female; D044950:Flavanones / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D066298:In Vitro Techniques; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction; D008941:Spindle Apparatus / Q000187:drug effects; D013552:Swine
chemical_list: C119737:8-prenylnaringenin; D044950:Flavanones; C005273:naringenin
keywords: 8-Prenylnaringenin; Embryo development; Lipid droplets; Naringenin; Oocyte maturation