title: Characterization of a degradable polar hydrophobic ionic polyurethane with circulating angiogenic cells in vitro.
abstract: This study investigated the interaction of human circulating angiogenic cells (CACs) with a degradable polar hydrophobic ionic polyurethane (D-PHI) which has been previously shown to exhibit anti-inflammatory character and favorable interactions with human endothelial cells (ECs). Given the implication of the CACs in microvessel development it was of intrinsic interest to expand our knowledge of D-PHI biocompatibility with this relevant primary cell involved in angiogenesis. The findings will be compared to a well-established benchmark substrate for CACs, fibronectin-coated tissue culture polystyrene (TCPS). Immunoblotting analysis showed that CACs were a heterogeneous population of cells composed mostly of monocytic cells expressing the CD14 marker. Assessment of the cytokine release profile, using ELISA, showed that D-PHI supported a higher concentration of interleukin-10 (IL-10) when compared to the concentration of tumor necrosis factor alpha, which is indicative of an anti-inflammatory phenotype, and was different from the response with TCPS. It was found that the CACs were attached to D-PHI and remained viable and functional (nitric oxide production) during the seven days of culture. However, there did not appear to be any significant proliferation on D-PHI, contrary to the CAC growth on fibronectin-coated TCPS. It was concluded that D-PHI displayed some of the qualities suitable to enable the retention of CACs onto this substrate, as well as maintaining an anti-inflammatory phenotype, characteristics which have been reported to be important for angiogenesis in vivo.
authors.affiliation: a Division of Cardiac Surgery , University of Ottawa Heart Institute , Ottawa , Canada.
authors.forename: Eva
authors.initials: E
authors.lastname: Mathieu
authors.forename: Kyle G
authors.initials: KG
authors.lastname: Battiston
authors.forename: Joanne E
authors.initials: JE
authors.lastname: McBane
authors.forename: Lien
authors.initials: L
authors.lastname: Davidson
authors.forename: Erik J
authors.initials: EJ
authors.lastname: Suuronen
authors.forename: J Paul
authors.initials: JP
authors.lastname: Santerre
authors.forename: Rosalind S
authors.initials: RS
authors.lastname: Labow
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D016207:Cytokines / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011140:Polyurethanes / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D005353:Fibronectins; D018950:Lipopolysaccharide Receptors; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011140:Polyurethanes; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009569:Nitric Oxide
keywords: circulating angiogenic cells; degradable; monocytes; polyurethane; vascular graft