title: Enzymatic stability and immunoregulatory efficacy of a synthetic indolicidin analogue with regular enantiomeric sequence.
abstract: Cell-mediated immunity plays a major role in protecting the host from viral infections and tumor challenge. Here, we report the enzymatic stability and adjuvanticity of a peptiomimetic stereoisomer of the bovine neutrophil peptide indolicidin. The analogue, dubbed ld-indolicidin, contains the regular enantiomeric sequence of indolicidin and is synthesized by general stepwise solid-phase strategy. ld-Indolicidin possesses high resistance to enzymatic degradation and shows tolerance in mice. As vaccine adjuvant, ld-indolicidin is better able than the native form of indolicidin to enhance cell-mediated immune responses, using inactivated H5N1 virus as a model antigen. Taken together, these results open up a new approach to the development of vaccine adjuvants and immunotherapy technologies.
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 35053, Taiwan.
authors.forename: Ching-Yun
authors.initials: CY
authors.lastname: Chang
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 35053, Taiwan.
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Lin
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 35053, Taiwan.
authors.forename: Sheng-Kuo
authors.initials: SK
authors.lastname: Chiang
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 35053, Taiwan.
authors.forename: Po-Ling
authors.initials: PL
authors.lastname: Chen
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 35053, Taiwan.
authors.forename: Chiung-Yi
authors.initials: CY
authors.lastname: Huang
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 35053, Taiwan ; Graduate Institute of Immunology, China Medical University , Taichung 40402, Taiwan.
authors.forename: Shih-Jen
authors.initials: SJ
authors.lastname: Liu
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 35053, Taiwan ; Graduate Institute of Immunology, China Medical University , Taichung 40402, Taiwan.
authors.forename: Pele
authors.initials: P
authors.lastname: Chong
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 35053, Taiwan ; Biotechnology Center, National Chung Hsing University , Taichung 40227, Taiwan.
authors.forename: Ming-Hsi
authors.initials: MH
authors.lastname: Huang
keywords: Host defense peptides; immunoregulation; peptidomimetic stereoisomer; vaccine adjuvants