title: Guaiane sesquiterpenes and isopimarane diterpenes from an endophytic fungus Xylaria sp.
abstract: Nine oxygenated guaiane-type sesquiterpenes and three isopimarane diterpenes were isolated from the culture broth of an endophytic fungus, Xylaria sp. YM 311647, obtained from Azadirachta indica. The structures of these compounds were elucidated by interpretation of spectroscopic data. The absolute configurations of two of these were confirmed by X-ray crystallographic analysis. All of the compounds were tested for their antifungal activities against five pathogenic fungal cells. The results showed that nine sesquiterpenes were moderately active against Candida albicans and Hormodendrum compactum with MIC values ranging from 32 to 256μg/ml, while the diterpenes were more active; One of these exhibited the most potent inhibitory activity against C. albicans and Pyricularia oryzae with MIC values of 16μg/ml.
authors.affiliation: Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, People's Republic of China. Electronic address: shwu123@126.com.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Wu
authors.affiliation: School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China. Electronic address: jianhe2005@gmail.com.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China.
authors.forename: Xiao-Nian
authors.initials: XN
authors.lastname: Li
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.affiliation: Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Song
authors.affiliation: Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, People's Republic of China.
authors.forename: You-Wei
authors.initials: YW
authors.lastname: Chen
authors.affiliation: Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, People's Republic of China.
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Miao
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031601:Azadirachta / Q000382:microbiology; D002176:Candida albicans / Q000187:drug effects; D002681:China; D018360:Crystallography, X-Ray; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D045789:Sesquiterpenes, Guaiane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013098:Xylariales / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D004224:Diterpenes; D045789:Sesquiterpenes, Guaiane
keywords: Antifungal activity; Azadirachta indica; Diterpene; Guaiane; Isopimarane; Sesquiterpene; Xylaria sp.