title: Effects of internalized gold nanoparticles with respect to cytotoxicity and invasion activity in lung cancer cells.
abstract: The effect of gold nanoparticles on lung cancer cells is not yet clear. In this study, we investigated the cytotoxicity and cell invasion activity of lung cancer cells after treatment with gold nanoparticles and showed that small gold nanoparticles can be endocytosed by lung cancer cells and that they facilitate cell invasion. The growth of A549 cells was inhibited after treatment with 5-nm gold nanoparticles, but cell invasion increased. Endocytosed gold nanoparticles (size, 10 nm) notably promoted the invasion activity of 95D cells. All these effects of gold nanoparticles were not seen after treatment with larger particles (20 and 40 nm). The enhanced invasion activity may be associated with the increased expression of matrix metalloproteinase 9 and intercellular adhesion molecule-1. In this study, we obtained evidence for the effect of gold nanoparticles on lung cancer cell invasion activity in vitro. Moreover, matrix metalloproteinase 9 and intercellular adhesion molecule-1, key modulators of cell invasion, were found to be regulated by gold nanoparticles. These data also demonstrate that the responses of the A549 and 95D cells to gold nanoparticles have a remarkable relationship with their unique size-dependent physiochemical properties. Therefore, this study provides a new perspective for cell biology research in nanomedicine.
authors.affiliation: Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical University, Jiangsu, China.
authors.forename: Zhengxia
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical University, Jiangsu, China.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical University, Jiangsu, China.
authors.forename: Zhirui
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical University, Jiangsu, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical University, Jiangsu, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical University, Jiangsu, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical University, Jiangsu, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Lu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D019343:Citric Acid / Q000737:chemistry; D004705:Endocytosis / Q000187:drug effects*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008175:Lung Neoplasms; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D010316:Particle Size
chemical_list: D018799:Intercellular Adhesion Molecule-1; D019343:Citric Acid; D006046:Gold; D020780:Matrix Metalloproteinase 9