title: An insight into the cytotoxic activity of phytol at in vitro conditions.
abstract: The cytotoxicity of the diterpene alcohol, phytol, was evaluated by using the MTT assay in vitro against seven tumour cells and one normal cell of human origin. The compound tested induced concentration-dependent cytotoxic response in all cell lines, demonstrating to be most and least effective against the breast adenocarcinoma MCF-7 and the prostate adenocarcinoma PC-3 cells, respectively (IC50 8.79 ± 0.41 μM and 77.85 ± 1.93 μM). The IC50 values towards the other five tumours (HeLa, HT-29, A-549, Hs294T and MDA-MB-231) ranged from 15.51 to 69.67 μM. However, mild toxicity was detected against the foetal lung fibroblast MRC-5 cells at the concentrations used (IC50 124.84 ± 1.59 μM). According to the experimental data obtained, this cost-effective natural product widely present in the biosphere may inspire the development of new drug-like substances with improved cytotoxic activity on breast cancer.
authors.affiliation: a Department of Life Sciences , Institute for Multidisciplinary Research - IMSI, University of Belgrade , Belgrade , Serbia.
authors.forename: Boris
authors.initials: B
authors.lastname: Pejin
authors.forename: Vesna
authors.initials: V
authors.lastname: Kojic
authors.forename: Gordana
authors.initials: G
authors.lastname: Bogdanovic
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D008297:Male; D015394:Molecular Structure; D010836:Phytol / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D010836:Phytol
keywords: MCF-7 cells; MTT assay; diterpene alcohol