title: Storage of volume-reduced washed platelets in M-sol additive solution for 7 days.
abstract: BACKGROUND
  Volume-reduced washed platelets (VR-wPLTs), which are prepared by concentrating platelets (PLTs) into a smaller volume of additive solution (AS), may prevent not only circulatory overload, but also adverse reactions caused by plasma components. Although VR-wPLTs may be quickly degraded due to high PLT concentrations, few studies have examined the effects of storage on VR-wPLTs. We examined here the in vitro properties of VR-wPLTs prepared with M-sol AS during their storage for 7 days.
  
  
  METHODS
  Platelet concentrates (PCs) were divided into two equal aliquots (control group and test group). After the centrifugation of both aliquots and removal of as much supernatant as possible, the pellet of the control group was resuspended in 160 mL of M-sol while that of the test group was resuspended in 80 or 40 mL of M-sol. The wPLTs of both groups were stored in polyolefin bags with agitation at 20 to 24°C for 7 days.
  
  
  RESULTS
  The pH values of both groups were maintained at higher than 7.0 during the 7-day storage. Differences in %disk, CD62P, annexin V, percent hypotonic shock response, and aggregation values between the test group and control group were small for at least 2 days after washing.
  
  
  CONCLUSIONS
  The in vitro properties of VR-wPLTs were not markedly degraded for at least 2 days. Therefore, the storage properties of PLTs may be maintained in VR-wPLTs prepared at blood centers until they are administered to patients in hospitals.
authors.affiliation: Japanese Red Cross Hokkaido Block Blood Center, Sapporo, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Hirayama
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Fujihara
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Akino
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kojima
authors.forename: Ryu
authors.initials: R
authors.lastname: Yanagisawa
authors.forename: Chihiro
authors.initials: C
authors.lastname: Homma
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kato
authors.forename: Hisami
authors.initials: H
authors.lastname: Ikeda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Azuma
authors.forename: Shigetaka
authors.initials: S
authors.lastname: Shimodaira
authors.forename: Shigeru
authors.initials: S
authors.lastname: Takamoto
mesh_terms: D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D019999:Pharmaceutical Solutions / Q000494:pharmacology; D013997:Time Factors
chemical_list: D019999:Pharmaceutical Solutions