title: Crystallization of dicalcium phosphate dihydrate with presence of glutamic acid and arginine at 37 °C.
abstract: The formations of non-metabolic stones, bones and teeth were seriously related to the morphology, size and surface reactivity of dicalcium phosphate dihydrate (DCPD). Herein, a facile biomimetic mineralization method with presence of glutamic acid and arginine was employed to fabricate DCPD with well-defined morphology and adjustable crystallite size. In reaction solution containing more arginine, crystallization of DCPD occurred with faster rate of nucleation and higher density of stacked layers due to the generation of more OH(-) ions after hydrolysis of arginine at 37 °C. With addition of fluorescein or acetone, the consumption of OH(-) ions or desolvation reaction of Ca(2+) ions was modulated, which resulted in the fabrication of DCPD with adjustable crystallite sizes and densities of stacked layers. In comparison with fluorescein-loading DCPD, dicalcium phosphate anhydrate was prepared with enhanced photoluminescence properties due to the reduction of self-quenching effect and regular arrangement of encapsulated fluorescein molecules. With addition of more acetone, DCPD was prepared with smaller crystallite size via antisolvent crystallization. The simulated process with addition of amino acids under 37 °C would shed light on the dynamic process of biomineralization for calcium phosphate compounds.
authors.affiliation: School of Materials Science and Engineering, Shandong University of Technology, 255049 Shandong, PR China. Electronic address: cfli@sdut.edu.cn.
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Science, Shandong University of Technology, 255049 Shandong, PR China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Ge
authors.affiliation: School of Materials Science and Engineering, Shandong University of Technology, 255049 Shandong, PR China.
authors.forename: Guochang
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Materials Science and Engineering, Shandong University of Technology, 255049 Shandong, PR China.
authors.forename: Jiahai
authors.initials: J
authors.lastname: Bai
authors.affiliation: School of Materials Science and Engineering, Shandong University of Technology, 255049 Shandong, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ding
mesh_terms: D001120:Arginine / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D003460:Crystallization; D019793:Fluorescein / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry*; D006878:Hydroxides / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D002130:Calcium Phosphates; D006878:Hydroxides; D018698:Glutamic Acid; C031356:hydroxide ion; D001120:Arginine; C494366:calcium phosphate, dibasic, dihydrate; D002118:Calcium; D019793:Fluorescein
keywords: Antisolvent crystallization; Arginine; Biomimetic mineralization; Dicalcium phosphate dihydrate; Glutamic acid