title: Functional surface engineering by nucleotide-modulated potassium channel insertion into polymer membranes attached to solid supports.
abstract: Planar solid-supported membranes based on amphiphilic block copolymers represent promising systems for the artificial creation of structural surfaces. Here we introduce a method for engineering functional planar solid-supported membranes through insertion of active biomolecules. We show that membranes based on poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (PDMS-b-PMOXA) amphiphilic diblock copolymers, which mimic natural membranes, are suitable for hosting biomolecules. Our strategy allows preparation of large-area, well-ordered polymer bilayers via Langmuir-Blodgett and Langmuir-Schaefer transfers, and insertion of biomolecules by using Bio-Beads. We demonstrate that a model membrane protein, the potassium channel from the bacterium Mesorhizobium loti, remains functional after insertion into the planar solid-supported polymer membrane. This approach can be easily extended to generate a platform of functional solid-supported membranes by insertion of different hydrophobic biomolecules, and employing different types of solid substrates for desired applications.
authors.affiliation: Chemistry Department, University of Basel, Klingelbergstrasse 80, 4056 Basel, Switzerland.
authors.forename: Justyna Ł
authors.initials: JŁ
authors.lastname: Kowal
authors.affiliation: Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Mattenstrasse 26, 4058 Basel, Switzerland.
authors.forename: Julia K
authors.initials: JK
authors.lastname: Kowal
authors.affiliation: Chemistry Department, University of Basel, Klingelbergstrasse 80, 4056 Basel, Switzerland.
authors.forename: Dalin
authors.initials: D
authors.lastname: Wu
authors.affiliation: Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Mattenstrasse 26, 4058 Basel, Switzerland.
authors.forename: Henning
authors.initials: H
authors.lastname: Stahlberg
authors.affiliation: Chemistry Department, University of Basel, Klingelbergstrasse 80, 4056 Basel, Switzerland.
authors.forename: Cornelia G
authors.initials: CG
authors.lastname: Palivan
authors.affiliation: Chemistry Department, University of Basel, Klingelbergstrasse 80, 4056 Basel, Switzerland. Electronic address: wolfgang.meier@unibas.ch.
authors.forename: Wolfgang P
authors.initials: WP
authors.lastname: Meier
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism*; D008567:Membranes, Artificial; D059128:Mesorhizobium / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D009711:Nucleotides / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry*; D015221:Potassium Channels / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013499:Surface Properties
chemical_list: D001426:Bacterial Proteins; D004129:Dimethylpolysiloxanes; D055767:Immobilized Proteins; D008567:Membranes, Artificial; D009711:Nucleotides; D011073:Polyamines; D015221:Potassium Channels; C117403:poly(2-methyl-2-oxazoline)
keywords: Amphiphilic block copolymers; Biomolecule insertion; Functional surfaces; Membrane protein; Planar solid-supported polymer membranes