title: Probing nanoparticles and nanoparticle-conjugated biomolecules using time-of-flight secondary ion mass spectrometry.
abstract: Bio-conjugated nanoparticles have emerged as novel molecular probes in nano-biotechnology and nanomedicine and chemical analyses of their surfaces have become challenges. The time-of-flight (TOF) secondary ion mass spectrometry (SIMS) has been one of the most powerful surface characterization techniques for both nanoparticles and biomolecules. When combined with various nanoparticle-based signal enhancing strategies, TOF-SIMS can probe the functionalization of nanoparticles as well as their locations and interactions in biological systems. Especially, nanoparticle-based SIMS is an attractive approach for label-free drug screening because signal-enhancing nanoparticles can be designed to directly measure the enzyme activity. The chemical-specific imaging analysis using SIMS is also well suited to screen nanoparticles and nanoparticle-biomolecule conjugates in complex environments. This review presents some recent applications of nanoparticle-based TOF-SIMS to the chemical analysis of complex biological systems.
authors.affiliation: Department of Life Science, Institute of Nano Science and Technology, and Research Institute for Natural Sciences, Hanyang University, Seoul, 133-791, Republic of Korea.
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Kim
authors.forename: Hyun Kyong
authors.initials: HK
authors.lastname: Shon
authors.forename: Seung Koo
authors.initials: SK
authors.lastname: Shin
authors.forename: Tae Geol
authors.initials: TG
authors.lastname: Lee
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015704:CD4 Antigens / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*; D045663:Quantum Dots / Q000737:chemistry; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*
chemical_list: D000906:Antibodies; D015704:CD4 Antigens; D018796:Immunoconjugates; D010455:Peptides; D006046:Gold; D052203:Ferrosoferric Oxide
keywords: TOF-SIMS; biomolecule; mass imaging; nanoparticle; signal enhancement; surface characterization