title: Detecting senescence: methods and approaches.
abstract: The detection of senescent cells has become an important area of research in the aging field. Due to the complexity of the senescence program and the lack of a unique signature for senescence, the detection of these cells remains problematic. This is especially true for in vivo detection in aged or diseased tissue samples. This chapter outlines approaches for the detection of senescent cells based upon methods established for mesenchymal cells in culture. A stepwise approach to the detection of senescent cells using multiple techniques is provided.
authors.affiliation: Department of Pathology, Drexel University College of Medicine, 245 N. 15th Street, MS 435, Philadelphia, PA, 19102, USA.
authors.forename: Elizabeth P
authors.initials: EP
authors.lastname: Crowe
authors.forename: Timothy
authors.initials: T
authors.lastname: Nacarelli
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bitto
authors.forename: Chad
authors.initials: C
authors.lastname: Lerner
authors.forename: Christian
authors.initials: C
authors.lastname: Sell
authors.forename: Claudio
authors.initials: C
authors.lastname: Torres
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation; D016922:Cellular Senescence; D015925:Cryopreservation / Q000379:methods; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique / Q000379:methods; D006570:Heterochromatin; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D006570:Heterochromatin; D001616:beta-Galactosidase