title: Antitumoral effect of a selective Rho-kinase inhibitor Y-27632 against Ehrlich ascites carcinoma in mice.
abstract: BACKGROUND
  The Rho proteins and Rho-kinase (ROCK) enzymes are responsible for signal transduction, and cause cell permeability, contractility, differentiation, migration, proliferation or apoptosis depending on cell types. All of these functions are vital for cancer initiation and progression. In this study, the preventive and protective effects of a selective ROCK inhibitor Y-27632 against Ehrlich ascites carcinoma in Swiss albino mice were investigated.
  
  
  METHODS
  Adult male albino mice were divided into five equal groups, and Y-27632 (0.1, 1, and 10 mg/kg) was given to groups as two steps; before (pre-carcinoma) and after inoculation of carcinoma cell suspensions (post-carcinoma). At the end of the experiments (at day 15), cardiac blood samples, the ascitic fluid, and intestinal specimens were collected for histopathology and biochemical investigation.
  
  
  RESULTS
  Significant decreases in the body weight and immunostaining scores in small and large intestine for ROCK2, preservation of serum glutathione (GSH) levels, and an increase in tumor level of nitric oxide were recorded in groups pretreated with Y-27632. However, treatment with Y-27632 after tumor inoculation did not affect body weight and ROCK2 immunostaining scores, increased serum MDA levels, and decreased GSH levels.
  
  
  CONCLUSIONS
  This is the first study on the effectiveness of Y-27632 in this experimental tumor model. Our findings provided direct evidence for ROCK involvement in tumor development. These data suggest that pretreatment with Y-27632 has a protective effect against tumor formation.
authors.affiliation: Department of Biology, Faculty of Art and Sciences, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Derya
authors.initials: D
authors.lastname: Isler
authors.affiliation: Department of Biology, Faculty of Art and Sciences, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozaslan
authors.affiliation: Department of Biology, Faculty of Art and Sciences, University of Gaziantep, Gaziantep, Turkey. Electronic address: karagoz@gantep.edu.tr.
authors.forename: Isik Didem
authors.initials: ID
authors.lastname: Karagoz
authors.affiliation: Department of Biology, Faculty of Art and Sciences, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Ibrahim Halil
authors.initials: IH
authors.lastname: Kilic
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Karakok
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Seyithan
authors.initials: S
authors.lastname: Taysi
authors.affiliation: Department of Biology, Faculty of Art and Sciences, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Izzettin
authors.initials: I
authors.lastname: Guler
authors.affiliation: Department of Biology, Faculty of Art and Sciences, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Cakmak
authors.affiliation: Department of Medical Pharmacology, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Abdullah Tuncay
authors.initials: AT
authors.lastname: Demiryurek
mesh_terms: D000577:Amides / Q000627:therapeutic use*; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D007422:Intestines / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D011725:Pyridines / Q000627:therapeutic use*; D054460:rho-Associated Kinases / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000502:physiology
chemical_list: D000577:Amides; D000970:Antineoplastic Agents; D011725:Pyridines; C108830:Y 27632; D008315:Malondialdehyde; C515855:Rock2 protein, mouse; D054460:rho-Associated Kinases; D005978:Glutathione
keywords: Ehrlich ascites carcinoma; Experimental tumor; Rho-kinase; Y-27632