title: Engineering of cyanobacteria for the photosynthetic production of limonene from CO2.
abstract: Isoprenoids, major secondary metabolites in many organisms, are utilized in various applications. We constructed a model photosynthetic production system for limonene, a volatile isoprenoid, using a unicellular cyanobacterium that expresses the plant limonene synthase. This system produces limonene photosynthetically at a nearly constant rate and that can be efficiently recovered using a gas-stripping method. This production does not affect the growth of the cyanobacteria and is markedly enhanced by overexpression of three enzymes in the intrinsic pathway to provide the precursor of limonene, geranyl pyrophosphate. The photosynthetic production of limonene in our system is more or less sustained from the linear to stationary phase of cyanobacterial growth for up to 1 month.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyota
authors.affiliation: Department of Life Sciences (Biology), Graduate School of Arts and Science, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Okuda
authors.affiliation: Graduate School and Faculty of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimo-Adachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Michiho
authors.initials: M
authors.lastname: Ito
authors.affiliation: RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Masami Yokota
authors.initials: MY
authors.lastname: Hirai
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Life Sciences (Biology), Graduate School of Arts and Science, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan; Japan Science and Technology Agency (JST), CREST, Saitama, Japan. Electronic address: mikeuchi@bio.c.u-tokyo.ac.jp.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikeuchi
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics*; D015153:Blotting, Western; D002245:Carbon Dioxide / Q000378:metabolism*; D053138:Cyclohexenes / Q000737:chemistry*; D004896:Erythritol / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D005818:Genetic Engineering / Q000379:methods*; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism*; D019686:Lamiaceae / Q000201:enzymology; D000077222:Limonene; D010788:Photosynthesis / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013403:Sugar Phosphates / Q000235:genetics / Q000378:metabolism; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism*; D013729:Terpenes / Q000737:chemistry*
chemical_list: C114232:2-C-methylerythritol 4-phosphate; D053138:Cyclohexenes; D011106:Polyisoprenyl Phosphates; D013403:Sugar Phosphates; D013729:Terpenes; D002245:Carbon Dioxide; C015234:geranyl pyrophosphate; D000077222:Limonene; D019753:Intramolecular Lyases; C040265:pinene cyclase I; D004896:Erythritol
keywords: Isoprenoid; Limonene; MEP pathway; Synechocystis; Synthetic biology