title: Corolla is a novel protein that contributes to the architecture of the synaptonemal complex of Drosophila.
abstract: In most organisms the synaptonemal complex (SC) connects paired homologs along their entire length during much of meiotic prophase. To better understand the structure of the SC, we aim to identify its components and to determine how each of these components contributes to SC function. Here, we report the identification of a novel SC component in Drosophila melanogaster female oocytes, which we have named Corolla. Using structured illumination microscopy, we demonstrate that Corolla is a component of the central region of the SC. Consistent with its localization, we show by yeast two-hybrid analysis that Corolla strongly interacts with Cona, a central element protein, demonstrating the first direct interaction between two inner-synaptonemal complex proteins in Drosophila. These observations help provide a more complete model of SC structure and function in Drosophila females.
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Collins
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors.forename: Jay R
authors.initials: JR
authors.lastname: Unruh
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Slaughter
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors.forename: Zulin
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors.forename: Cathleen M
authors.initials: CM
authors.lastname: Lake
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors.forename: Rachel J
authors.initials: RJ
authors.lastname: Nielsen
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors.forename: Kimberly S
authors.initials: KS
authors.lastname: Box
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110 Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas 66160.
authors.forename: Danny E
authors.initials: DE
authors.lastname: Miller
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas 66045.
authors.forename: Justin P
authors.initials: JP
authors.lastname: Blumenstiel
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors.forename: Anoja G
authors.initials: AG
authors.lastname: Perera
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Malanowski
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110 Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas 66160 rsh@stowers.org.
authors.forename: R Scott
authors.initials: RS
authors.lastname: Hawley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D011485:Protein Binding; D013573:Synaptonemal Complex / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; C532575:cona protein, Drosophila; C000599611:corolla protein, Drosophila
keywords: chromosome segregation; meiosis; next-generation sequencing; structured illumination microscopy; synaptonemal complex