title: Optimization for ultrasound-assisted extraction of polysaccharides with antioxidant activity in vitro from the aerial root of Ficus microcarpa.
abstract: In this study, optimization of ultrasound-assisted extraction and antioxidant activity of polysaccharides from the aerial root of Ficus microcarpa (FMPS) were investigated. The optimal conditions for extraction of FMPS were determined as followings: ultrasound power 200 W, ultrasound temperature 70°C, extraction temperature 74°C, liquid-solid ratio 35, extraction time 238 min, ultrasound time 49 min. The experimental yield of FMPS (3.44%) obtained under these conditions was well agreement with the value predicted by the model. In addition, Fourier transform-infrared spectroscopy and antioxidant activity assays revealed that FMPS were acidic polysaccharides and had strong Fe2+ chelating activity and moderate hydrogen peroxide scavenging effect. Further work on the purification, structure characterization and antioxidant activity in vivo of FMPS is in progress.
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Changxing
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Food Engineering Department, Jiangsu Food & Pharmaceutical Science College, Huaian 223003, Jiangsu, PR China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Dingyun
authors.initials: D
authors.lastname: Jiang
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Benxia
authors.initials: B
authors.lastname: Fan
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China. Electronic address: qianghuazhang1234@gmail.com.
authors.forename: Qianghua
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000528:radiation effects*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000528:radiation effects*; D030681:Ficus; D035261:Plant Components, Aerial; D018517:Plant Roots; D011134:Polysaccharides / Q000302:isolation & purification / Q000528:radiation effects*; D013016:Sound
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides
keywords: Antioxidant activity; Ficus microcarpa; Polysaccharide; Response surface methodology; Ultrasound-assisted extraction