title: The fruits of wampee inhibit H2O2-induced apoptosis in PC12 cells via the NF-κB pathway and regulation of cellular redox status.
abstract: Wampee (Clausena lansium) fruits (CLS), whose pulp can be used to prepare fruit cups, desserts, jam, or jelly, can be eaten along with the peel. In this study, a PC12 cell model was built to observe the protective effect of CLS against H2O2-induced oxidative stress. We found that pretreatment with CLS increased cell viability and inhibited cytotoxicity, caspase-3 activity and DNA condensation. CLS also attenuated the increase in ROS production and MMP reduction. Moreover, we attempted to determine whether CLS suppressed the expression and phosphorylation of NF-κB. Western blot and immunostaining assay revealed that CLS inhibited H2O2-induced up-regulation of NF-κB p65 and pNF-κB p65. And CLS significantly suppressed the translocation of NF-κB p65 and pNF-κB p65 from cytoplasm to nuclear. Also, seven major compounds including a new flavanoid, luteolin-4'-O-β-d-gluco-pyranoside (3) and six known compounds 1,2, 4-7 were isolated and identified from CLS. Their antioxidative and H2O2-induced PC12 cell apoptosis-reversing activity were determined. These findings suggest that CLS and its major constituents (flavanoids) may be potential antioxidant agents and should encourage further research into their use as a functional food for neurodegenerative diseases.
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524023, Guangdong, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524023, Guangdong, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524023, Guangdong, China.
authors.forename: Liao
authors.initials: L
authors.lastname: Cui
authors.affiliation: Wuzhou Red Cross Hospital, Wuzhou 510663, Guangxi, China.
authors.forename: Decheng
authors.initials: D
authors.lastname: Liu
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524023, Guangdong, China. winokhere@sina.com.
authors.forename: Kefeng
authors.initials: K
authors.lastname: Wu
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524023, Guangdong, China. wli18@partners.org.
authors.forename: Wende
authors.initials: W
authors.lastname: Li
authors.affiliation: Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, Guangdong, China.
authors.forename: Ren
authors.initials: R
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D054700:Clausena / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D016716:PC12 Cells; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D016328:NF-kappa B; D010936:Plant Extracts; D006861:Hydrogen Peroxide