title: Comparative analysis of 11 Brassicales mitochondrial genomes and the mitochondrial transcriptome of Brassica oleracea.
abstract: To elucidate the evolution of mitochondrial genomic diversity within a single order of angiosperms, we sequenced seven Brassicales genomes and the transcriptome of Brassica oleracea. In the common ancestor of Brassicaceae, several genes of known function were lost and the ccmFN gene was split into two independent genes, which also coincides with a trend of genome reduction towards the smallest sequenced angiosperm genomes of Brassica. For most ORFs of unknown function, the lack of conservation throughout Brassicales and the generally low expression and absence of RNA editing in B. oleracea argue against functionality. However, two chimeric ORFs were expressed and edited in B. oleracea, suggesting a potential role in cytoplasmic male sterility in certain nuclear backgrounds. These results demonstrate how frequent shifts in size, structure, and content of plant mitochondrial genomes can occur over short evolutionary time scales.
authors.affiliation: Center for Plant Science Innovation, University of Nebraska, Lincoln, NE 68588, USA; Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68583, USA.
authors.forename: Felix
authors.initials: F
authors.lastname: Grewe
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Patrick P
authors.initials: PP
authors.lastname: Edger
authors.affiliation: Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Givat-Ram, Jerusalem 91904, Israel.
authors.forename: Ido
authors.initials: I
authors.lastname: Keren
authors.affiliation: Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Givat-Ram, Jerusalem 91904, Israel.
authors.forename: Laure
authors.initials: L
authors.lastname: Sultan
authors.affiliation: Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.
authors.forename: J Chris
authors.initials: JC
authors.lastname: Pires
authors.affiliation: Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Givat-Ram, Jerusalem 91904, Israel.
authors.forename: Oren
authors.initials: O
authors.lastname: Ostersetzer-Biran
authors.affiliation: Center for Plant Science Innovation, University of Nebraska, Lincoln, NE 68588, USA; Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68583, USA.
authors.forename: Jeffrey P
authors.initials: JP
authors.lastname: Mower
mesh_terms: D019607:Brassicaceae / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D014644:Genetic Variation; D054629:Genome, Mitochondrial; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D004272:DNA, Mitochondrial
keywords: Brassicales; Genome evolution; Plant mitochondria; RNA editing