title: NMR as evaluation strategy for cellular uptake of nanoparticles.
abstract: Advanced nanostructured materials, such as gold nanoparticles, magnetic nanoparticles, and multifunctional materials, are nowadays used in many state-of-the-art biomedical application. However, although the engineering in this field is very advanced, there remain some fundamental problems involving the interaction mechanisms between nanostructures and cells or tissues. Here we show the potential of (1)H NMR in the investigation of the uptake of two different kinds of nanostructures, that is, maghemite and gold nanoparticles, and of a chemotherapy drug (Temozolomide) in glioblastoma tumor cells. The proposed experimental protocol provides a new way to investigate the general problem of cellular uptake for a variety of biocompatible nanostructures and drugs.
authors.affiliation: Department of Physics and INSTM Unit, University of Pavia , Pavia, Italy.
authors.forename: Tomas
authors.initials: T
authors.lastname: Orlando
authors.forename: Alessandro
authors.initials: A
authors.lastname: Paolini
authors.forename: Francesco
authors.initials: F
authors.lastname: Pineider
authors.forename: Emanuela
authors.initials: E
authors.lastname: Clementi
authors.forename: Francesca
authors.initials: F
authors.lastname: Pasi
authors.forename: Yannick
authors.initials: Y
authors.lastname: Guari
authors.forename: Joulia
authors.initials: J
authors.lastname: Larionova
authors.forename: Luciano
authors.initials: L
authors.lastname: Sacchi
authors.forename: Rosanna
authors.initials: R
authors.lastname: Nano
authors.forename: Maurizio
authors.initials: M
authors.lastname: Corti
authors.forename: Alessandro
authors.initials: A
authors.lastname: Lascialfari
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D003606:Dacarbazine / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D005290:Ferric Compounds / Q000378:metabolism*; D005909:Glioblastoma / Q000188:drug therapy; D006046:Gold / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D053758:Nanoparticles / Q000378:metabolism*; D012210:Rhamnose / Q000378:metabolism; D000077204:Temozolomide
chemical_list: D018906:Antineoplastic Agents, Alkylating; D005290:Ferric Compounds; C000499:ferric oxide; D006046:Gold; D003606:Dacarbazine; D012210:Rhamnose; D000077204:Temozolomide