title: A DFT and ONIOM study of C-H hydroxylation catalyzed by nitrobenzene 1,2-dioxygenase.
abstract: A detailed description of the mechanism of C-H hydroxylation by Rieske non-heme iron dioxygenases remains elusive, as the nature of the oxidizing species is not definitively known. DFT calculations on cluster models of nitrobenzene 1,2-dioxygenase were done to explore possible mechanisms arising from oxidation by either the experimentally observed Fe(III)-OOH complex or the putative high-valent HO-Fe(V)=O intermediate formed through a heterolytic O-O bond cleavage. Hydrogen abstraction by HO-Fe(V)=O, followed by oxygen rebound, was found to be consistent with experimental studies. The findings from the quantum mechanical cluster approach were verified by accounting for the effect of the protein environment on transition state geometries and reaction barriers through ONIOM calculations.
authors.affiliation: Institute of Applied Radiation Chemistry, Lodz University of Technology, Żeromskiego 116, 90-924 Łódź, Poland. paneth@p.lodz.pl.
authors.forename: Inacrist
authors.initials: I
authors.lastname: Geronimo
authors.forename: Piotr
authors.initials: P
authors.lastname: Paneth
mesh_terms: D002244:Carbon / Q000737:chemistry*; D003198:Computer Simulation; D049308:Dioxygenases / Q000737:chemistry* / Q000648:ultrastructure*; D006859:Hydrogen / Q000737:chemistry*; D006900:Hydroxylation; D008956:Models, Chemical; D008958:Models, Molecular; D008968:Molecular Conformation; D009578:Nitrobenzenes / Q000737:chemistry*; D011789:Quantum Theory
chemical_list: D009578:Nitrobenzenes; D002244:Carbon; D006859:Hydrogen; C036077:nitrobenzene; D049308:Dioxygenases