title: Molecular characterization of dengue viruses circulating during 2009-2012 in Uttar Pradesh, India.
abstract: Dengue is the most rapidly spreading mosquito-borne viral disease in the world; in India it has taken endemic proportion implicating all the four known dengue virus serotypes. Dengue infection is caused by a small, single stranded RNA virus comprising of four antigenically distinct virus serotypes designated as dengue virus type 1-4 (DENV-1-4). On the basis of genomic variations, each serotype is classified further into its genotypes. Epidemiological studies have shown that the emergence of a newer dengue serotype/genotype after an interval always leads to a major outbreak; therefore a continuous epidemiological surveillance is needed to monitor the epidemiology of dengue viruses. The present study was planned to identify the serotype/genotype of dengue viruses circulating in Uttar Pradesh, India. Of 433 dengue suspected patients, tested by reverse transcriptase PCR (RT-PCR), 136 were positive for dengue virus RNA. Of these, DENV-1, 2, and 3 were detected in 26 (19.1%), 77 (56.6%), and 33 (24.3%) patients, respectively. Of 136 RT-PCR positive samples, 24 samples were sequenced to identify their genotypes. For sequencing C-prM gene junction of dengue virus genome was chosen. Phylogenetic analysis of sequenced dengue strains revealed that all the 12 DENV-1 strains were genotype III, all the eight DENV-2 strains were genotype IV (Cosmopolitan genotype) and among four DENV-3 strains, three were genotype III and one was genotype I. In conclusion, the co-circulation of multiple dengue virus serotypes and genotypes is alarming in U.P., India.
authors.affiliation: Department of Microbiology, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Gitika
authors.initials: G
authors.lastname: Mishra
authors.forename: Amita
authors.initials: A
authors.lastname: Jain
authors.forename: Om
authors.initials: O
authors.lastname: Prakash
authors.forename: Shantanu
authors.initials: S
authors.lastname: Prakash
authors.forename: Rashmi
authors.initials: R
authors.lastname: Kumar
authors.forename: Ravindra K
authors.initials: RK
authors.lastname: Garg
authors.forename: Nidhi
authors.initials: N
authors.lastname: Pandey
authors.forename: Mastan
authors.initials: M
authors.lastname: Singh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011446:Prospective Studies; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D065288:Serogroup; D055815:Young Adult
chemical_list: D012367:RNA, Viral
keywords: C-prM; DENV; epidemiology; phylogeny; reverse transcriptase PCR; sequencing