title: Identification and characterization of a halotolerant, cold-active marine endo-β-1,4-glucanase by using functional metagenomics of seaweed-associated microbiota.
abstract: A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-β-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions.
authors.affiliation: Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium marjolaine.martin@ulg.ac.be.
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Martin
authors.affiliation: Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium.
authors.forename: Sophie
authors.initials: S
authors.lastname: Biver
authors.affiliation: Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Steels
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Barbeyron
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France.
authors.forename: Murielle
authors.initials: M
authors.lastname: Jam
authors.affiliation: Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium.
authors.forename: Daniel
authors.initials: D
authors.lastname: Portetelle
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France.
authors.forename: Gurvan
authors.initials: G
authors.lastname: Michel
authors.affiliation: Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium.
authors.forename: Micheline
authors.initials: M
authors.lastname: Vandenbol
mesh_terms: D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D003080:Cold Temperature; D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D056186:Metagenomics; D064307:Microbiota; D008969:Molecular Sequence Data; D000459:Phaeophyceae / Q000382:microbiology*; D010802:Phylogeny; D012624:Seaweed / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012965:Sodium Chloride; D006026:Glycoside Hydrolases