title: Identification of constitutively active AtMPK6 mutants using a functional screen in Saccharomyces cerevisiae.
abstract: MAPK (Mitogen-Activated Protein Kinases) mutants which are active independently of phosphorylation by upstream MAPK Kinases (MAPKKs) help to clarify signal transduction processes through MAPK modules and provide a useful tool to understand MAPK roles in the cell. The identification of such mutations is tricky. In this chapter, we provide a detailed protocol for their screening, taking advantage of a functional expression assay in yeast.
authors.affiliation: Institut de Biologie des Plantes, UMR CNRS-8618, Centre National De La Recherche Scientifique, Rue de Noetzlin, Bâtiment 630, 91405, Orsay Cedex, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Hudik
authors.forename: Souha
authors.initials: S
authors.lastname: Berriri
authors.forename: Heribert
authors.initials: H
authors.lastname: Hirt
authors.forename: Jean
authors.initials: J
authors.lastname: Colcombet
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics*; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D012492:Salts / Q000494:pharmacology; D014170:Transformation, Genetic
chemical_list: D029681:Arabidopsis Proteins; D012492:Salts; C436293:MPK6 protein, Arabidopsis; D020928:Mitogen-Activated Protein Kinases