title: Performance comparison of four exome capture systems for deep sequencing.
abstract: BACKGROUND
  Recent developments in deep (next-generation) sequencing technologies are significantly impacting medical research. The global analysis of protein coding regions in genomes of interest by whole exome sequencing is a widely used application. Many technologies for exome capture are commercially available; here we compare the performance of four of them: NimbleGen's SeqCap EZ v3.0, Agilent's SureSelect v4.0, Illumina's TruSeq Exome, and Illumina's Nextera Exome, all applied to the same human tumor DNA sample.
  
  
  RESULTS
  Each capture technology was evaluated for its coverage of different exome databases, target coverage efficiency, GC bias, sensitivity in single nucleotide variant detection, sensitivity in small indel detection, and technical reproducibility. In general, all technologies performed well; however, our data demonstrated small, but consistent differences between the four capture technologies. Illumina technologies cover more bases in coding and untranslated regions. Furthermore, whereas most of the technologies provide reduced coverage in regions with low or high GC content, the Nextera technology tends to bias towards target regions with high GC content.
  
  
  CONCLUSIONS
  We show key differences in performance between the four technologies. Our data should help researchers who are planning exome sequencing to select appropriate exome capture technology for their particular application.
authors.affiliation: Department of Tumor Biology, Oslo University Hospital, Norwegian Radium Hospital, 0310 Oslo, Norway. chichi@rr-research.no.
authors.forename: Chandra Sekhar Reddy
authors.initials: CS
authors.lastname: Chilamakuri
authors.forename: Susanne
authors.initials: S
authors.lastname: Lorenz
authors.forename: Mohammed-Amin
authors.initials: MA
authors.lastname: Madoui
authors.forename: Daniel
authors.initials: D
authors.lastname: Vodák
authors.forename: Jinchang
authors.initials: J
authors.lastname: Sun
authors.forename: Eivind
authors.initials: E
authors.lastname: Hovig
authors.forename: Ola
authors.initials: O
authors.lastname: Myklebost
authors.forename: Leonardo A
authors.initials: LA
authors.lastname: Meza-Zepeda
mesh_terms: D001482:Base Composition; D059472:Exome; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods* / Q000592:standards*; D006801:Humans; D054643:INDEL Mutation; D009369:Neoplasms / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results