title: Molecular detection of Bartonella spp. in deer ked pupae, adult keds and moose blood in Finland.
abstract: The deer ked (Lipoptena cervi) is a haematophagous ectoparasite of cervids that harbours haemotrophic Bartonella. A prerequisite for the vector competence of the deer ked is the vertical transmission of the pathogen from the mother to its progeny and transstadial transmission from pupa to winged adult. We screened 1154 pupae and 59 pools of winged adult deer keds from different areas in Finland for Bartonella DNA using PCR. Altogether 13 pupa samples and one winged adult deer ked were positive for the presence of Bartonella DNA. The amplified sequences were closely related to either B. schoenbuchensis or B. bovis. The same lineages were identified in eight blood samples collected from free-ranging moose. This is the first demonstration of Bartonella spp. DNA in a winged adult deer ked and, thus, evidence for potential transstadial transmission of Bartonella spp. in the species.
authors.affiliation: University of Helsinki, Haartman Institute,Department of Virology, Helsinki,Finland.
authors.forename: E M
authors.initials: EM
authors.lastname: Korhonen
authors.affiliation: University of Helsinki, Haartman Institute,Department of Virology, Helsinki,Finland.
authors.forename: C
authors.initials: C
authors.lastname: Pérez Vera
authors.affiliation: Institute of Biomedicine, Medical Biochemistry and Genetics,University of Turku,Turku,Finland.
authors.forename: A T
authors.initials: AT
authors.lastname: Pulliainen
authors.affiliation: University of Helsinki, Haartman Institute,Department of Virology, Helsinki,Finland.
authors.forename: T
authors.initials: T
authors.lastname: Sironen
authors.affiliation: University of Helsinki, Haartman Institute,Department of Virology, Helsinki,Finland.
authors.forename: K
authors.initials: K
authors.lastname: Aaltonen
authors.affiliation: University of Eastern Finland,Department of Biology, Joensuu,Finland.
authors.forename: R
authors.initials: R
authors.lastname: Kortet
authors.affiliation: University of Oulu,Department of Biology, Oulu,Finland.
authors.forename: L
authors.initials: L
authors.lastname: Härkönen
authors.affiliation: Finnish Forest Research Institute,Joensuu Research Unit, Joensuu,Finland.
authors.forename: S
authors.initials: S
authors.lastname: Härkönen
authors.affiliation: University of Eastern Finland,Department of Biology, Joensuu,Finland.
authors.forename: T
authors.initials: T
authors.lastname: Paakkonen
authors.affiliation: University of Eastern Finland,Department of Biology, Joensuu,Finland.
authors.forename: P
authors.initials: P
authors.lastname: Nieminen
authors.affiliation: University of Eastern Finland,Department of Biology, Joensuu,Finland.
authors.forename: A-M
authors.initials: AM
authors.lastname: Mustonen
authors.affiliation: University of Jyväskylä,Department of Biological and Environmental Science, Konnevesi Research Station, Jyväskylä,Finland.
authors.forename: H
authors.initials: H
authors.lastname: Ylönen
authors.affiliation: University of Helsinki, Haartman Institute,Department of Virology, Helsinki,Finland.
authors.forename: O
authors.initials: O
authors.lastname: Vapalahti
mesh_terms: D000818:Animals; D001473:Bartonella / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D003670:Deer / Q000469:parasitology*; D004175:Diptera / Q000382:microbiology*; D005387:Finland; D016133:Polymerase Chain Reaction; D011679:Pupa / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial
keywords: Bartonella; Lipoptena cervi; deer ked; moose; zoonosis