title: Measurement of atropisomer racemization kinetics using segmented flow technology.
abstract: When stable atropisomers are encountered by drug discovery teams, they can have important implications due to potential differences in their biological activity, pharmacokinetics, and toxicity. Knowledge of an atropisomer's activation parameters for interconversion is required to facilitate informed decisions on how to proceed. Herein, we communicate the development of a new method for the rapid measurement of atropisomer racemization kinetics utilizing segmented flow technology. This method leverages the speed, accuracy, low sample requirement, safety, and semiautomated nature of flow instrumentation to facilitate the acquisition of kinetics data required for experimentally probing atropisomer activation parameters. Measured kinetics data obtained for the atropo isomerization of AMPA antagonist CP-465021 using segmented flow and traditional thermal methods were compared to validate the method.
authors.affiliation: Neuroscience Chemistry, Pfizer Global Research and Development , Groton, Connecticut 06340, United States.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Davoren
authors.affiliation: Neuroscience Chemistry, Pfizer Global Research and Development , Groton, Connecticut 06340, United States.
authors.forename: Mark W
authors.initials: MW
authors.lastname: Bundesmann
authors.affiliation: Neuroscience Chemistry, Pfizer Global Research and Development , Groton, Connecticut 06340, United States.
authors.forename: Qi T
authors.initials: QT
authors.lastname: Yan
authors.affiliation: Neuroscience Chemistry, Pfizer Global Research and Development , Groton, Connecticut 06340, United States.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Collantes
authors.affiliation: Neuroscience Chemistry, Pfizer Global Research and Development , Groton, Connecticut 06340, United States.
authors.forename: Scot
authors.initials: S
authors.lastname: Mente
authors.affiliation: Neuroscience Chemistry, Pfizer Global Research and Development , Groton, Connecticut 06340, United States.
authors.forename: Deane M
authors.initials: DM
authors.lastname: Nason
authors.affiliation: Neuroscience Chemistry, Pfizer Global Research and Development , Groton, Connecticut 06340, United States.
authors.forename: David L
authors.initials: DL
authors.lastname: Gray
keywords: Atropisomer; Reaction kinetics; Segmented flow chemistry