title: Production and Characterization of Protein Encapsulated Silver Nanoparticles by Marine Isolate Streptomyces parvulus SSNP11.
abstract: Production of protein encapsulated silver nanoparticles (AgNPs) assisted by marine actinomycetes strain has been investigated. The selective isolate was identified as Streptomyces parvulus SSNP11 based on chemotaxonomic and 16S rRNA analysis. Maximum AgNPs production was observed within 24 h incubation time. The produced AgNPs are spherical in shape with monodispersive and crystalline in nature. The particle size distribution ranges from 1.66 to 11.68 nm with a mean size of 2.1 nm. The biosynthesized AgNPs revealed stretching vibrations of primary and secondary amines along with C-H and C-N, suggesting that metabolically produced proteins are involved in size regulation of reduced AgNPs. These particles possess an average negative zeta potential value of 81.5 mV with an electrophoretic mobility of 0.000628 cm(2)/Vs. The biosynthesized nanoparticles revealed antimicrobial property against gram negative as well as gram positive bacterial strains.
authors.affiliation: Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India.
authors.forename: Reddy Shetty
authors.initials: RS
authors.lastname: Prakasham
authors.affiliation: Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India.
authors.forename: Buddana Sudheer
authors.initials: BS
authors.lastname: Kumar
authors.affiliation: Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India.
authors.forename: Yannam Sudheer
authors.initials: YS
authors.lastname: Kumar
authors.affiliation: Therdose PharmaPvt Limited, Hyderabad, India.
authors.forename: Katikala Prasanth
authors.initials: KP
authors.lastname: Kumar
keywords: FT-IR spectroscopy; Silver nanoparticles; Streptomyces parvulus SSNP11; Zeta potential