title: Retention of polyphenolic species in spray-dried blackberry extract using mannitol as a thermoprotectant.
abstract: The purpose of these studies was to determine if a Büchi Mini Spray Dryer B-290 (Büchi Corporation, New Castle, DE, USA) could be used to prepare blackberry extract powders containing mannitol as a thermoprotectant without extensively degrading anthocyanins and polyphenols in the resulting powders. Three blackberry puree extract samples were each prepared by sonication of puree in 30/70% ethanol/water containing 0.003% HCl. Blackberry puree extract sample 1 (S1) contained no mannitol, while blackberry puree extract sample 2 (S2) contained 3.0:1 (w/w) mannitol:berry extract, and blackberry puree extract sample 3 (S3) contained 6.3:1 (w/w) mannitol:berry extract. The levels of anthocyanins and polyphenols in reconstituted spray-dried powders produced from S1-S3 were compared to solutions of S1-S3 that were held at 4°C as controls. All extract samples could be spray-dried using the Büchi Mini Spray Dryer B-290. S1, with no mannitol, showed a 30.8% decrease in anthocyanins and a 24.1% decrease in polyphenols following spray-drying. However, S2 had a reduction in anthocyanins of only 13.8%, while polyphenols were reduced by only 6.1%. S3, with a ratio of mannitol to berry extract of 6.3:1, exhibited a 12.5% decrease in anthocyanins while the decrease in polyphenols after spray-drying was not statistically significant (P=.16). Collectively, these data indicate that a Büchi Mini Spray Dryer B-290 is a suitable platform for producing stable berry extract powders, and that mannitol is a suitable thermoprotectant that facilitates retention of thermosensitive polyphenolic species in berry extracts during spray-drying.
authors.affiliation: 1 Four Tigers LLC , Lexington, Kentucky.
authors.forename: Joshua A
authors.initials: JA
authors.lastname: Eldridge
authors.forename: Debra
authors.initials: D
authors.lastname: Repko
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Mumper
mesh_terms: D003890:Desiccation; D004355:Drug Stability; D005638:Fruit / Q000737:chemistry; D006358:Hot Temperature; D008353:Mannitol / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D011208:Powders / Q000737:chemistry; D011310:Preservatives, Pharmaceutical / Q000737:chemistry*; D065856:Rubus / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D059808:Polyphenols; D011208:Powders; D011310:Preservatives, Pharmaceutical; D008353:Mannitol
keywords: anthocyanins; carriers; phenolics; powder; stability; thermal protectant