title: An efficient nanomaterial-based electrochemical biosensor for sensitive recognition of drug-resistant leukemia cells.
abstract: A novel electrochemical cytosensor was developed for the fast and high-sensitivity recognition of drug-resistant leukemia K562/ADM cells based on the P-glycoprotein (P-gp) expression level on a cell membrane. The nanocomposite interface of the gold nanoparticles/polyaniline nanofibers (AuNPs/PANI-NF) was chosen to design the biosensor for electrochemical detection. Au/PANI-NF-based cytosensors coated with anti-P-glycoprotein (anti-P-gp) molecules could provide a biomimetic interface for the immunosensing of cell surface P-glycoprotein, and thus could capture the over-expression P-gp cells. Transmission electron microscopy (TEM) indicated that the gold nanoparticles were uniformly anchored along the structure of the PANI-NF surface, displaying fibrillar morphology with a diameter of ∼70 nm, and atomic force microscopy (AFM) further presented the morphology of the nanocomposite film. Owing to the high affinity of anti-P-gp for leukemia K562/ADM cells of the propounded sensing platform, the proposed biosensor exhibited excellent analytical performance for leukemia K562/ADM cells, ranging from 1.6 × 10(2) to 1.6 × 10(6) cells per mL with a detection limit of 80 cells per mL. Recovery experiments indicated that the sensitivity reported here is suitable for practical application. The cell surface P-gp expression level was analysed by flow cytometric experiments, which confirmed the above recognized result. This strategy is also a cost-effective and convenient operation, implying great promise for the sensitive recognition of cancer cells and cell surface receptors; thus, it is helpful in cancer diagnosis.
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China. typh@jnu.edu.cn zhangshaolian1990@163.com.
authors.forename: Shaolian
authors.initials: S
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Peihui
authors.initials: P
authors.lastname: Yang
authors.forename: Jiye
authors.initials: J
authors.lastname: Cai
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis*; D000814:Aniline Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D019008:Drug Resistance, Neoplasm; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000175:diagnosis* / Q000188:drug therapy; D053761:Nanocomposites / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000814:Aniline Compounds; C416807:polyaniline; D006046:Gold