title: Analyzing cell cycle-dependent degradation and ubiquitination in budding yeast.
abstract: Cell cycle progression is tightly regulated to prevent uncontrolled growth and division. Specific cell cycle factors are responsible for driving the cell from one cell cycle stage to the next. Many of these proteins are targeted for degradation by the ubiquitin proteasome system when their function is no longer required or may disrupt cell cycle progression. Here we describe a series of experiments used to study the ubiquitin-mediated degradation of cell cycle proteins. This collection of assays may be used to determine the requirement for individual proteins in the degradation and ubiquitination of cell cycle proteins in Saccharomyces cerevisiae.
authors.affiliation: Department of Genetics, Cell Biology and Development, University of Minnesota, 6-160 Jackson Hall, 321 Church Street SE, Minneapolis, MN, 55455, USA.
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Kim
authors.forename: Deanna M
authors.initials: DM
authors.lastname: Koepp
mesh_terms: D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D047468:Immunoprecipitation / Q000379:methods; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D055550:Protein Stability; D059748:Proteolysis; D004718:Saccharomycetales / Q000166:cytology / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex