title: Follicular Viability and Histological Alterations after Auto-transplantation of Dog Ovaries by Experimentally Inducing Blood Sinus on Stomach.
abstract: BACKGROUND
  Currently, chemotherapy and radiotherapy are considered most effective methods for cancer treatment, however these strategies often result in fertility problems. A favorable alternative to prevent fertility loss in cancer patients is the cryopreservation and transplantation of sexual tissues (ovaries and/or testes). There is a low rate of fertilization following cryopreservation of ovaries prior to implantation. Therefore, in our opinion, this low rate is caused by instable blood flow during organ transplantation. Thus, this study researches a canine ovarian model that focuses on direct exposure of ovaries with blood in an experimentally induced sinus-like cavity. We implanted this tissue on the muscular layer of the stomach, which is its most vascularized region.
  
  
  METHODS
  Ovarian transplantation was conducted on T1 animals (n=5), bilateral ovariectomy was performed on T2 animals (n=5), unilateral ovariectomy was conducted on T3 cases and animals in the control-sham group (n=5) did not undergo ovariectomy or transplantation.
  
  
  RESULTS
  All isotransplanted ovaries survived. Ovaries resumed follicular growth and revascularization. Transplanted ovaries contained 75%-76% of survived small follicles (pre antral) after 60 days. The ovarian granulosa cells showed considerable resistance against ischemia. After day 30 no statistically significant differences in the level of estradiol and progesterone were observed between T1 animals and the T3 group. T1 animals showed considerably high levels of progesterone and estradiol in comparison to T2 cases.
  
  
  CONCLUSIONS
  This study showed that using blood sinus method for ovarian isotransplantation helps ovarian tissue to survive from post implantation ischemia which confirms with normal follicles presentation and intact endocrine function of the implanted ovaries.
authors.affiliation: Clinical Science Department, Faculty of Veterinary Medicine, Islamic Azad University, Urmia Branch, Urmia, Iran.
authors.forename: Hazhir
authors.initials: H
authors.lastname: Khoram
authors.affiliation: Clinical Science Department, Faculty of Veterinary Medicine, Islamic Azad University, Urmia Branch, Urmia, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Najafpour
authors.affiliation: Comparative Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors.forename: Mazdak
authors.initials: M
authors.lastname: Razi
keywords: Canine; Ovarian; Sexual Hormone; Transplantation