title: Analyzing RB and E2F during the G1-S transition.
abstract: The G1/S-phase restriction point is an important landmark in the mammalian cell division cycle. The key regulator of the G1/S transition is the retinoblastoma gene product (pRB). It prevents the transcription of genes required for S-phase progression by repressing E2F transcription factors. An increase in Cdk phosphorylation of pRB causes the release of E2F transcription factors and advancement into S phase. Here we describe two simple techniques used to assess pRB phosphorylation and E2F transcription during G1/S progression.
authors.affiliation: London Regional Cancer Program, London, ON, Canada.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Thwaites
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Cecchini
authors.forename: Frederick A
authors.initials: FA
authors.lastname: Dick
mesh_terms: D000818:Animals; D050684:E2F Transcription Factors / Q000378:metabolism*; D004867:Equipment Design; D016193:G1 Phase; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods; D010766:Phosphorylation; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D016160:Retinoblastoma Protein / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D050684:E2F Transcription Factors; D016160:Retinoblastoma Protein; D012313:RNA