title: Molecular characterization of Rickettsia massiliae and Anaplasma platys infecting Rhipicephalus sanguineus ticks and domestic dogs, Buenos Aires (Argentina).
abstract: Rickettsioses, ehrlichioses and anaplasmoses are emerging diseases that are mainly transmitted by arthropods and that affect humans and animals. The aim of the present study was to use molecular techniques to detect and characterize those pathogens in dogs and ticks from Buenos Aires city. We studied 207 Rhipicephalus sanguineus ticks and 52 canine blood samples from poor neighborhoods of Buenos Aires city. The samples were molecularly screened for the genera Rickettsia, Ehrlichia, and Anaplasma by PCR and sequencing. DNA of Rickettsia massiliae (3.4%) and Anaplasma platys (13.5%) was detected in ticks and blood samples, respectively. For characterization, the positive samples were subjected to amplification of a fragment of the 190-kDa outer membrane protein gene (spotted fever group rickettsiae) and a fragment of the groESL gene (specific for A. platys). A phylogenetic tree was constructed using the neighbor-joining method, revealing that the sequences were closely related to those of strains from other geographic regions. The results indicate that human and animal pathogens are abundant in dogs and their ticks in Buenos Aires city and portray the potentially high risk of human exposure to infection with these agents, especially in poor neighborhoods, where there is close contact with animals in an environment of poor health conditions.
authors.affiliation: Instituto de Zoonosis Luis Pasteur, Ministerio de Salud, Buenos Aires City, Argentina. Electronic address: gcicuttin@gmail.com.
authors.forename: Gabriel L
authors.initials: GL
authors.lastname: Cicuttin
authors.affiliation: Instituto de Zoonosis Luis Pasteur, Ministerio de Salud, Buenos Aires City, Argentina.
authors.forename: Diego F
authors.initials: DF
authors.lastname: Brambati
authors.affiliation: Instituto de Zoonosis Luis Pasteur, Ministerio de Salud, Buenos Aires City, Argentina.
authors.forename: Juan I
authors.initials: JI
authors.lastname: Rodríguez Eugui
authors.affiliation: Instituto de Zoonosis Luis Pasteur, Ministerio de Salud, Buenos Aires City, Argentina.
authors.forename: Cecilia González
authors.initials: CG
authors.lastname: Lebrero
authors.affiliation: Instituto de Zoonosis Luis Pasteur, Ministerio de Salud, Buenos Aires City, Argentina.
authors.forename: María N
authors.initials: MN
authors.lastname: De Salvo
authors.affiliation: Instituto de Zoonosis Luis Pasteur, Ministerio de Salud, Buenos Aires City, Argentina.
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Beltrán
authors.affiliation: Instituto de Zoonosis Luis Pasteur, Ministerio de Salud, Buenos Aires City, Argentina.
authors.forename: Federico E
authors.initials: FE
authors.lastname: Gury Dohmen
authors.affiliation: Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Jado
authors.affiliation: Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Anda
mesh_terms: D000709:Anaplasma / Q000235:genetics*; D000712:Anaplasmosis / Q000453:epidemiology / Q000469:parasitology*; D000818:Animals; D001118:Argentina / Q000453:epidemiology; D004283:Dog Diseases / Q000453:epidemiology / Q000382:microbiology* / Q000469:parasitology; D004285:Dogs; D010802:Phylogeny; D048508:Rhipicephalus sanguineus / Q000382:microbiology*; D012281:Rickettsia / Q000235:genetics*; D012282:Rickettsia Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013984:Tick Infestations / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D017282:Tick-Borne Diseases / Q000453:epidemiology / Q000382:microbiology / Q000469:parasitology / Q000662:veterinary
keywords: Anaplasma platys; Buenos Aires; Dogs; Rhipicephalus sanguineus; Rickettsia massiliae