title: Response surface optimization of ultrasound-assisted enzymatic extraction polysaccharides from Lycium barbarum.
abstract: In this study, an efficient ultrasound-assisted enzymatic extraction procedure for the water-soluble polysaccharides from the fruit of Lycium barbarum was investigated and optimized. Response surface methodology (RSM) based on a three-level four-factor Box Behnken Design (BBD) was employed to optimize the extraction conditions inlcluding extraction time, ultrasonic output power, cellulose concentration and extraction temperature. The experimental data were adequately fitted into a second-order polynomial model. The optimized conditions were as follows: extraction time 20.29 min, ultrasonic output power 78.6 W, cellulose concentration 2.15%, extraction temperature 55.79°C. Under these conditions, the experimental yield of polysaccharides was 6.31±0.03%, which matched with the predictive yield of 6.32% well.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China; School of Chemistry & Chemical Engineering, Tianjin University of Technology, Tianjin, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China.
authors.forename: Guili
authors.initials: G
authors.lastname: Gong
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China.
authors.forename: Shaoyi
authors.initials: S
authors.lastname: Jia
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China. Electronic address: 18222703069@163.com.
authors.forename: Songhai
authors.initials: S
authors.lastname: Wu
mesh_terms: D055162:Biocatalysis; D005638:Fruit / Q000737:chemistry; D032305:Lycium / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D013010:Sonication
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides
keywords: Lycium barbarum polysaccharides; Optimization; Response surface methodology; Ultrasound-assisted enzymatic extraction