title: Significance of satellite DNA revealed by conservation of a widespread repeat DNA sequence among angiosperms.
abstract: The analysis of plant genome structure and evolution requires comprehensive characterization of repetitive sequences that make up the majority of plant nuclear DNA. In the present study, we analyzed the nature of pCtKpnI-I and pCtKpnI-II tandem repeated sequences, reported earlier in Carthamus tinctorius. Interestingly, homolog of pCtKpnI-I repeat sequence was also found to be present in widely divergent families of angiosperms. pCtKpnI-I showed high sequence similarity but low copy number among various taxa of different families of angiosperms analyzed. In comparison, pCtKpnI-II was specific to the genus Carthamus and was not present in any other taxa analyzed. The molecular structure of pCtKpnI-I was analyzed in various unrelated taxa of angiosperms to decipher the evolutionary conserved nature of the sequence and its possible functional role.
authors.affiliation: Department of Botany, University of Delhi, Delhi, 110007, India, shwetamehrotra@rediffmail.com.
authors.forename: Shweta
authors.initials: S
authors.lastname: Mehrotra
authors.forename: Shailendra
authors.initials: S
authors.lastname: Goel
authors.forename: Soom Nath
authors.initials: SN
authors.lastname: Raina
authors.forename: Vijay Rani
authors.initials: VR
authors.lastname: Rajpal
mesh_terms: D017124:Conserved Sequence; D018744:DNA, Plant / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics; D019684:Magnoliopsida / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D018744:DNA, Plant; D004276:DNA, Satellite