title: Folic acid-conjugated iron oxide porous nanorods loaded with doxorubicin for targeted drug delivery.
abstract: Iron oxide porous nanorods (IOPNR) with lengths ranging from 40nm to 60nm and pore diameters ranging from 5nm to 10nm were prepared, and further modified with NH2-PEG-FA (FA-PEG-IOPNR) for ligand targeting and modified with NH2-PEG-OCH3 (PEG-IOPNR) as a control. Instead of chemical bonding, doxorubicin (DOX), a low water solubility anticancer drug, was loaded in the pores of the modified IOPNR because of their porous structure and high porosity. The release of DOX in acidic PBS solution (pH 5.3) was faster than that in neutral (pH 7.4) solution. The analysis results from TEM, inductively coupled plasma emission spectroscopy, confocal laser scanning microscopy, and flow cytometry analyses indicated that the presence of FA on the surface of the nanorods increase the cellular uptake of nanorods in the case of HeLa cells, a folate receptor (FR)-positive cell line. In contrast, for COS 7 cells, a FR-negative cell line, FA ligand on the surface of the nanorods showed no effect on the cellular uptake. MTT assay indicated that the cytotoxicity of DOX loaded in FA-PEG-IOPNR to HeLa cells was higher than that of DOX in PEG-IOPNR. In the case of COS 7 cells, no significant difference between the cytotoxicity of DOX loaded in FA-PEG-IOPNR and PEG-IOPNR was found. These results suggested that FA-PEG-IOPNR had the potential for target delivery of chemotherapeutic into cancer cells.
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China.
authors.forename: Xi-Ming
authors.initials: XM
authors.lastname: Xia
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China.
authors.forename: Can
authors.initials: C
authors.lastname: Cui
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China.
authors.forename: Liu-Jie
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China.
authors.forename: Ren-Xi
authors.initials: RX
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China. Electronic address: swhuang@whu.edu.cn.
authors.forename: Shi-Wen
authors.initials: SW
authors.lastname: Huang
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004317:Doxorubicin / Q000494:pharmacology*; D016503:Drug Delivery Systems; D004705:Endocytosis / Q000187:drug effects; D005290:Ferric Compounds / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005492:Folic Acid / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014018:Tissue Distribution / Q000187:drug effects; D014961:X-Ray Diffraction
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D004317:Doxorubicin; D005492:Folic Acid; D016650:Fluorescein-5-isothiocyanate
keywords: Active targeting; Anti-cancer; Drug delivery; Porous nanorods