title: Multi-component analysis in sun-dried and sulfur-fumigated Angelicae Sinensis Radix by single marker quantitation and chemometric discrimination.
abstract: BACKGROUND
  A new method has been developed for the simultaneous determination of ferulic acid, senkyunolide A, and Z-ligustilide in Angelicae Sinensis Radix before and after sulfur-fumigation using quantitative analysis of multi-components by a single marker (QAMS).
  
  
  METHODS
  The feasibility and accuracy of QAMS were checked by the external standard method, and various high-performance liquid chromatographic instruments and chromatographic conditions were investigated to verify its applicability. Using ferulic acid as the internal reference substance, and the contents of senkyunolide A and Z-ligustilide were calculated according to relative correction factors by high-performance liquid chromatography. Meanwhile, the influence of sulfur-fumigation on these chemical components in Angelicae Sinensis Radix were evaluated and discriminated by chromatographic fingerprint and chemometrics.
  
  
  RESULTS
  There was no significant difference observed between the QAMS method and the external standard method. Furthermore, sulfur-fumigation reduced the contents of ferulic acid, senkyunolide A, and Z-ligustilide in Angelicae Sinensis Radix by some degree, and the sun-drying and sulfur-fumigation processing could be easily discriminated by chromatographic fingerprint and chemometrics.
  
  
  CONCLUSIONS
  QAMS is a convenient and accurate approach to analyzing multi-component when reference substances are unavailable, simultaneously, chemometrics is an effective way to discriminate sun-dried and sulfur-fumigated Angelicae Sinensis Radix.
authors.affiliation: Department of Chinese Materia Medica, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Lou
authors.affiliation: Department of Chinese Materia Medica, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; National First-Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of Chinese Materia Medica, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; National First-Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; National First-Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
authors.affiliation: Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Sicong
authors.initials: S
authors.lastname: Tu
authors.affiliation: Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Chinese Medicine, Nanjing, People's Republic of China.
authors.forename: Songlin
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Chinese Materia Medica, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Chinese Materia Medica, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China.
authors.forename: Kunming
authors.initials: K
authors.lastname: Qin
authors.affiliation: Department of Chinese Materia Medica, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; National First-Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou, People's Republic of China.
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
keywords: Angelicae Sinensis Radix; chemometrics; quantitative analysis of multi-components by a single marker; relative correction factor; sulfur-fumigation