title: Multiple systemic transplantations of human amniotic mesenchymal stem cells exert therapeutic effects in an ALS mouse model.
abstract: Amyotrophic lateral sclerosis (ALS) is an adult-onset progressive neurodegenerative disease involving degeneration of motor neurons in the central nervous system. Stem cell treatment is a potential therapy for this fatal disorder. The human amniotic membrane (HAM), an extremely rich and easily accessible tissue, has been proposed as an attractive material in cellular therapy and regenerative medicine because of its advantageous characteristics. In the present study, we evaluate the long-term effects of a cellular treatment by intravenous administration of human amniotic mesenchymal stem cells (hAMSCs) derived from HAM into a hSOD1(G93A) mouse model. The mice received systemic administration of hAMSCs or phosphate-buffered saline (PBS) at the onset, progression and symptomatic stages of the disease. hAMSCs were detected in the spinal cord at the final stage of the disease, in the form of isolates or clusters and were negative for β-tubulin III and GFAP. Compared with the treatment with PBS, multiple hAMSC transplantations significantly retarded disease progression, extended survival, improved motor function, prevented motor neuron loss and decreased neuroinflammation in mice. These findings demonstrate that hAMSC transplantation is a promising cellular treatment for ALS.
authors.affiliation: Department of Neurosurgery, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Sun
authors.forename: Zongliu
authors.initials: Z
authors.lastname: Hou
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Yang
authors.forename: Mingyao
authors.initials: M
authors.lastname: Meng
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Qingjian
authors.initials: Q
authors.lastname: Zou
authors.forename: Lujun
authors.initials: L
authors.lastname: Yang
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Chen
authors.forename: Huihui
authors.initials: H
authors.lastname: Chai
authors.forename: Huilin
authors.initials: H
authors.lastname: Zhong
authors.forename: Zara Zhuyun
authors.initials: ZZ
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Liangxue
authors.initials: L
authors.lastname: Lai
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Jiang
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Xiao
mesh_terms: D000650:Amnion / Q000166:cytology*; D000690:Amyotrophic Lateral Sclerosis / Q000503:physiopathology / Q000628:therapy*; D000818:Animals; D001522:Behavior, Animal; D002465:Cell Movement; D002470:Cell Survival; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000473:pathology; D007275:Injections, Intravenous; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D009043:Motor Activity; D009046:Motor Neurons / Q000473:pathology
chemical_list: D049452:Green Fluorescent Proteins