title: Differential targeting of VDAC3 mRNA isoforms influences mitochondria morphology.
abstract: Intracellular targeting of mRNAs has recently emerged as a prevalent mechanism to control protein localization. For mitochondria, a cotranslational model of protein import is now proposed in parallel to the conventional posttranslational model, and mitochondrial targeting of mRNAs has been demonstrated in various organisms. Voltage-dependent anion channels (VDACs) are the most abundant proteins in the outer mitochondrial membrane and the major transport pathway for numerous metabolites. Four nucleus-encoded VDACs have been identified in Arabidopsis thaliana. Alternative cleavage and polyadenylation generate two VDAC3 mRNA isoforms differing by their 3' UTR. By using quantitative RT-PCR and in vivo mRNA visualization approaches, the two mRNA variants were shown differentially associated with mitochondria. The longest mRNA presents a 3' extension named alternative UTR (aUTR) that is necessary and sufficient to target VDAC3 mRNA to the mitochondrial surface. Moreover, aUTR is sufficient for the mitochondrial targeting of a reporter transcript, and can be used as a tool to target an unrelated mRNA to the mitochondrial surface. Finally, VDAC3-aUTR mRNA variant impacts mitochondria morphology and size, demonstrating the role of mRNA targeting in mitochondria biogenesis.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, 67084 Strasbourg Cedex, France;
authors.forename: Morgane
authors.initials: M
authors.lastname: Michaud
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, 67084 Strasbourg Cedex, France;
authors.forename: Elodie
authors.initials: E
authors.lastname: Ubrig
authors.affiliation: Institut des Sciences du Végétal, Centre National de la Recherche Scientifique-Unité Propre de Recherche 2355, Saclay Plant Sciences Labex, 91198 Gif sur Yvette Cedex, France;Université Paris 7 Denis Diderot, Unité de Formation et de Recherche Sciences du Vivant, 75205 Paris Cedex 13, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Filleur
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, 67084 Strasbourg Cedex, France;
authors.forename: Mathieu
authors.initials: M
authors.lastname: Erhardt
authors.affiliation: Institut des Sciences du Végétal, Centre National de la Recherche Scientifique-Unité Propre de Recherche 2355, Saclay Plant Sciences Labex, 91198 Gif sur Yvette Cedex, France;Université Paris 7 Denis Diderot, Unité de Formation et de Recherche Sciences du Vivant, 75205 Paris Cedex 13, France.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Ephritikhine
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, 67084 Strasbourg Cedex, France;
authors.forename: Laurence
authors.initials: L
authors.lastname: Maréchal-Drouard
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, 67084 Strasbourg Cedex, France; anne-marie.duchene@ibmp-cnrs.unistra.fr.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Duchêne
mesh_terms: D020413:3' Untranslated Regions; D017360:Arabidopsis / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D017343:Genes, Plant; D008928:Mitochondria / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D018272:Porins / Q000378:metabolism; D021381:Protein Transport; D059368:RNA Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D050994:Voltage-Dependent Anion Channels / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D029681:Arabidopsis Proteins; D033681:Mitochondrial Membrane Transport Proteins; D018272:Porins; D059368:RNA Isoforms; D012333:RNA, Messenger; C000591479:VDAC3 protein, Arabidopsis; D050994:Voltage-Dependent Anion Channels
keywords: green RNA; mRNA localization; mRNA sorting; plant; porin