title: Isolation techniques for anthocyanidin 3,5-diglucosides and their related chemicals using supramolecules technique, and two solid-phase extraction cartridges.
abstract: Isolation techniques for anthocyanidin 3,5-diglucosides and their derivatives from three types of supramolecular pigments were newly developed using two SPE (Discovery DPA-6S and DSC-SCX) cartridges. Initially, malonylawobanin from commelinin supramolecular complex yielded 79.5% with 85.7% purity using DPA-6S, while DSC-SCX gave a rather lower yield (62.5%) of malonylawobanin with high purity (90.8%). Delphinidin 3,5-diglucoside and awobanin from acidic hydrolysis of commelinin were easily separated using DPA-6S. Secondary, artificial Perilla supramolecules (reconstructed from Perilla crude pigment, flavocommelin and Mg(2+)) result in a high purity of a malonylshisonin and shisonin mixture (96.1%) with DSC-SCX. Finally, succinylcyanin, which is a component of protocyanin, yielded 64.3% with 86.1% purity using DPA-6S and 51.8% with 74.1% purity using DSC-SCX. Moreover, cyanidin 3,5-diglucoside yielded 43.4% with 97.0% purity using DPA-6S after partial acidic hydrolysis of protocyanin.
authors.affiliation: The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Asada
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Koi
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Risa
authors.initials: R
authors.lastname: Arakawa
authors.affiliation: The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan.
authors.forename: Fengxian
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Sadaoka
authors.affiliation: The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan; Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan. Electronic address: tamura@ag.kagawa-u.ac.jp.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Tamura
mesh_terms: D000872:Anthocyanins / Q000737:chemistry*; D002621:Chemistry / Q000379:methods*; D005419:Flavonoids; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D031343:Perilla / Q000737:chemistry; D052616:Solid Phase Extraction
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D005960:Glucosides; C539639:delphinidin 3-O-(6-O-p-coumaroylglucoside)-5-O-(6-O-malonylglucoside)
keywords: Anthocyanidin 3 ;5-diglucosides; Discovery DPA-6S and DSC-SCX; Isolation technique; Supramolecule