title: A colorimetric and ratiometric fluorescent probe for distinguishing cysteine from biothiols in water and living cells.
abstract: A merocyanine-based highly selective colorimetric and ratiometric fluorescent probe is described for Cys detection in water and diluted deproteinized human serum. Upon reaction with Cys in aqueous buffer solution, the probe showed a dramatic color change from faint yellow to pink and remarkable ratiometric fluorescence enhancement signals were also observed, which are ascribed to an intramolecular charge transfer (ICT) process. This strategy was based on modulating the merocyanine π-electron system by conjugation and removal of the acrylate group to release the chromophore group, resulting in a specific colorimetry and fluorescence response. The probe has low cytotoxicity and good cell permeability. It is readily employed for assessing the change of the intracellular Cys level.
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, P. R. China. liuws@lzu.edu.cn.
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Han
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Shi
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Tang
authors.forename: Lizi
authors.initials: L
authors.lastname: Yang
authors.forename: Zuolin
authors.initials: Z
authors.lastname: Mou
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jinmin
authors.initials: J
authors.lastname: Shi
authors.forename: Chunyang
authors.initials: C
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.forename: Weisheng
authors.initials: W
authors.lastname: Liu
mesh_terms: D002470:Cell Survival; D003124:Colorimetry; D003545:Cysteine / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D004355:Drug Stability; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008856:Microscopy, Fluorescence; D011874:Radiometry; D013050:Spectrometry, Fluorescence; D014867:Water / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D014867:Water; D003545:Cysteine