title: Potential role of bacteria packaging by protozoa in the persistence and transmission of pathogenic bacteria.
abstract: Many pathogenic bacteria live in close association with protozoa. These unicellular eukaryotic microorganisms are ubiquitous in various environments. A number of protozoa such as amoebae and ciliates ingest pathogenic bacteria, package them usually in membrane structures, and then release them into the environment. Packaged bacteria are more resistant to various stresses and are more apt to survive than free bacteria. New evidence indicates that protozoa and not bacteria control the packaging process. It is possible that packaging is more common than suspected and may play a major role in the persistence and transmission of pathogenic bacteria. To confirm the role of packaging in the propagation of infections, it is vital that the molecular mechanisms governing the packaging of bacteria by protozoa be identified as well as elements related to the ecology of this process in order to determine whether packaging acts as a Trojan Horse.
authors.affiliation: Institut de Biologie Intégrative et des Systèmes, Université Laval Quebec City, QC, Canada ; Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec Quebec City, QC, Canada.
authors.forename: Alix M
authors.initials: AM
authors.lastname: Denoncourt
authors.affiliation: Institut de Biologie Intégrative et des Systèmes, Université Laval Quebec City, QC, Canada ; Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec Quebec City, QC, Canada.
authors.forename: Valérie E
authors.initials: VE
authors.lastname: Paquet
authors.affiliation: Institut de Biologie Intégrative et des Systèmes, Université Laval Quebec City, QC, Canada ; Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec Quebec City, QC, Canada ; Département de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval Quebec City, QC, Canada.
authors.forename: Steve J
authors.initials: SJ
authors.lastname: Charette
keywords: Legionella pneumophila; amoeba; bacteria packaging; multilamellar body; mycobacteria; persistence; protozoa; transmission