title: Mutation breeding of lycopene-producing strain Blakeslea trispora by a novel atmospheric and room temperature plasma (ARTP).
abstract: To improve the fermentation efficiency of lycopene, a plasma jet, driven by an active helium atom supplied with atmospheric and room temperature plasma (ARTP) biological breeding system, was used as a new method to generate mutations in Blakeslea trispora (-). After several rounds of screening, a mutant A5 with high concentration of lycopene and dry biomass was isolated, which showed a maximum lycopene concentration (26.4 ± 0.2 mg/g dry biomass) which was 55 % higher than the parent strain (16.9 ± 0.3 mg/g dry biomass) in the production of lycopene. Compared with parent strain, B. trispora A5 required less dissolved oxygen (10 % less than that of parent strain) to reach maximum concentration in a 5-L stirred tank reactor batch fermentation.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, and School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu Province, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Qiang
authors.forename: Feng
authors.initials: F
authors.lastname: Ling-ran
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Li
authors.initials: L
authors.lastname: Han-guang
authors.forename: Wang
authors.initials: W
authors.lastname: Lin
authors.forename: Zhou
authors.initials: Z
authors.lastname: Ya
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao-bin
mesh_terms: D019149:Bioreactors; D002338:Carotenoids / Q000096:biosynthesis*; D000077276:Lycopene; D009090:Mucorales / Q000235:genetics / Q000254:growth & development; D016296:Mutagenesis; D009154:Mutation
chemical_list: D002338:Carotenoids; D000077276:Lycopene