title: Sustained delivery of latanoprost by thermosensitive chitosan-gelatin-based hydrogel for controlling ocular hypertension.
abstract: Glaucoma is an irreversible ocular disease that may lead to progressive visual field loss and eventually to blindness with inadequately controlled intraocular pressure (IOP). Latanoprost is one of the most potent ocular hypotensive compounds, the current first-line therapy in glaucoma. However, the daily instillation required for efficacy and undesirable side-effects are major causes of treatment adherence failure and persistence in glaucoma therapy. In the present study, we developed an injectable thermosensitive chitosan/gelatin/glycerol phosphate (C/G/GP) hydrogel as a sustained-release system of latanoprost for glaucoma treatment. The latanoprost-loaded C/G/GP hydrogel can gel within 1min at 37°C. The results show a sustained release of latanoprost from C/G/GP hydrogel in vitro and in vivo. The latanoprost-loaded C/G/GP hydrogel showed a good in vitro and in vivo biocompatibility. A rabbit model of glaucoma was established by intravitreal injection of triamcinolone acetonide. After a single subconjunctival injection of latanoprost-loaded C/G/GP hydrogel, IOP was significantly decreased within 8days and then remained at a normal level. The results of the study suggest that latanoprost-loaded C/G/GP hydrogel may have a potential application in glaucoma therapy.
authors.affiliation: Department and Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan; Department of Education and Research, Taipei City Hospital, Taipei, Taiwan.
authors.forename: Yung-Hsin
authors.initials: YH
authors.lastname: Cheng
authors.affiliation: Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; National Yang-Ming University School of Medicine, Taipei, Taiwan; Division of Ophthalmology, National Yang-Ming University Hospital, I-Lan, Taiwan.
authors.forename: Kuo-Hsuan
authors.initials: KH
authors.lastname: Hung
authors.affiliation: Department of Education and Research, Taipei City Hospital, Taipei, Taiwan; Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Tung-Hu
authors.initials: TH
authors.lastname: Tsai
authors.affiliation: Department of Education and Research, Taipei City Hospital, Taipei, Taiwan; Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chia-Jung
authors.initials: CJ
authors.lastname: Lee
authors.affiliation: National Yang-Ming University School of Medicine, Taipei, Taiwan.
authors.forename: Ruy-Yu
authors.initials: RY
authors.lastname: Ku
authors.affiliation: National Yang-Ming University School of Medicine, Taipei, Taiwan.
authors.forename: Allen Wen-Hsiang
authors.initials: AW
authors.lastname: Chiu
authors.affiliation: Department and Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan; Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan. Electronic address: shchiou@vghtpe.gov.tw.
authors.forename: Shih-Hwa
authors.initials: SH
authors.lastname: Chiou
authors.affiliation: National Yang-Ming University School of Medicine, Taipei, Taiwan; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan. Electronic address: jlliu@vghtpe.gov.tw.
authors.forename: Catherine Jui-Ling
authors.initials: CJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D000959:Antihypertensive Agents / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics; D004353:Drug Evaluation, Preclinical; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D000077338:Latanoprost; D008297:Male; D009798:Ocular Hypertension / Q000188:drug therapy*; D011461:Prostaglandins F, Synthetic / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D014222:Triamcinolone Acetonide / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D000959:Antihypertensive Agents; D003692:Delayed-Action Preparations; D020100:Hydrogels; D011461:Prostaglandins F, Synthetic; D000077338:Latanoprost; D005780:Gelatin; D048271:Chitosan; D014222:Triamcinolone Acetonide
keywords: Drug delivery; Glaucoma; Hydrogel; Latanoprost