title: Effects of phenol and meta-cresol depletion on insulin analog stability at physiological temperature.
abstract: The stability of three commercial "fast-acting" insulin analogs, insulin lispro, insulin aspart, and insulin glulisine, was studied at various concentrations of phenolic preservatives (phenol and/or meta-cresol) during 9 days of incubation at 37 °C. The analysis by both size-exclusion and reversed-phase chromatography showed degradation of lispro and aspart that was inversely dependent on the concentration of phenolic preservatives. Insulin glulisine was much more stable than the other analogs and showed minimal degradation even in the absence of phenolic preservatives. With sedimentation velocity ultracentrifugation, we determined the preservatives' effect on the insulins' self-assembly. When depleted of preservatives, insulin glulisine dissociates from higher molecular weight species into a number of intermediate molecular weight species, in between monomer and hexamer, whereas insulin aspart and insulin lispro dissociate into monomers and dimers. Decreased stability of insulin lispro and insulin aspart seems to be because of the extent of dissociation when depleted of preservative. Insulin glulisine's dissociation to intermediate molecular weight species appears to help minimize its degradation during incubation at 37 °C.
authors.affiliation: Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, Colorado, 80045.
authors.forename: Brandon M
authors.initials: BM
authors.lastname: Teska
authors.forename: Javier
authors.initials: J
authors.lastname: Alarcón
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Pettis
authors.forename: Theodore W
authors.initials: TW
authors.lastname: Randolph
authors.forename: John F
authors.initials: JF
authors.lastname: Carpenter
mesh_terms: D003408:Cresols / Q000737:chemistry*; D004355:Drug Stability; D005079:Excipients / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000737:chemistry*; D007328:Insulin / Q000031:analogs & derivatives* / Q000737:chemistry; D061267:Insulin Aspart / Q000737:chemistry*; D061268:Insulin Lispro / Q000737:chemistry*; D019800:Phenol / Q000737:chemistry*; D066329:Protein Aggregates; D013696:Temperature
chemical_list: D003408:Cresols; D005079:Excipients; D007004:Hypoglycemic Agents; D007328:Insulin; D061268:Insulin Lispro; D066329:Protein Aggregates; D019800:Phenol; C479079:insulin glulisine; D061267:Insulin Aspart; C042041:3-cresol
keywords: drug-excipient interaction; excipients; peptides; protein aggregation; protein formulation; proteins; self-assembly; stability; stabilization