title: Immunostaining of oxidized DJ-1 in human and mouse brains.
abstract: DJ-1, the product of a causative gene of a familial form of Parkinson disease, undergoes preferential oxidation of Cys106 (cysteine residue at position 106) under oxidative stress. Using specific monoclonal antibodies against Cys106 oxidized DJ-1 (oxDJ-1), we examined oxDJ-1 immunoreactivity in brain sections from DJ-1 knockout and wild-type mice and in human brain sections from cases classified into different Lewy body stages of Parkinson disease and Parkinson disease with dementia. Oxidized DJ-1 immunoreactivity was prominently observed in neuromelanin-containing neurons and neuron processes of the substantia nigra; Lewy bodies also showed oxDJ-1 immunoreactivity. Oxidized DJ-1 was also detected in astrocytes in the striatum, in neurons and glia in the red nucleus, and in the inferior olivary nucleus, all of which are related to regulation of movement. These observations suggest the relevance of DJ-1 oxidation to homeostasis in multiple brain regions, including neuromelanin-containing neurons of the substantia nigra, and raise the possibility that oxDJ-1 levels might change during the progression of Lewy body-associated neurodegenerative diseases.
authors.affiliation: From the Systems Life Sciences (YS, KH, YM, NN) and Neuropathology (TM, YI), Department of Medical Life Systems, Faculty of Medical and Life Sciences, Doshisha University, Kyotanabe, Kyoto; Department of Neuropathology, Tokyo Metropolitan Institute of Gerontology (HH, SM); Laboratory of Systems Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo (OK-A, HI, TH); and Institute of Immunology Co Ltd (OK-A), Tokyo; Health Research Institute, National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka (YY, EN); and Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo (KT-N, HA), Japan.
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Saito
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Miyasaka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hatsuta
authors.forename: Kazuko
authors.initials: K
authors.lastname: Takahashi-Niki
authors.forename: Kojiro
authors.initials: K
authors.lastname: Hayashi
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Mita
authors.forename: Osamu
authors.initials: O
authors.lastname: Kusano-Arai
authors.forename: Hiroko
authors.initials: H
authors.lastname: Iwanari
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ariga
authors.forename: Takao
authors.initials: T
authors.lastname: Hamakubo
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Etsuo
authors.initials: E
authors.lastname: Niki
authors.forename: Shigeo
authors.initials: S
authors.lastname: Murayama
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ihara
authors.forename: Noriko
authors.initials: N
authors.lastname: Noguchi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D059290:Dopaminergic Neurons / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000378:metabolism; D008636:Mesencephalon / Q000166:cytology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D017072:Neostriatum / Q000166:cytology / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics* / Q000378:metabolism*; D016612:Paraffin Embedding; D054464:Peroxiredoxins; D000071617:Protein Deglycase DJ-1; D011994:Recombinant Proteins / Q000737:chemistry; D016622:Silver Staining; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013378:Substantia Nigra / Q000166:cytology / Q000378:metabolism
chemical_list: D000906:Antibodies; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D011994:Recombinant Proteins; D054464:Peroxiredoxins; C105131:PARK7 protein, human; C503466:PARK7 protein, mouse; D000071617:Protein Deglycase DJ-1