title: Separation and purification of bioactive botrallin and TMC-264 by a combination of HSCCC and semi-preparative HPLC from endophytic fungus Hyalodendriella sp. Ponipodef12.
abstract: Two dibenzo-α-pyrones, botrallin (1) and TMC-264 (2) were preparatively separated from crude ethyl acetate extract of the endophytic fungus Hyalodendriella sp. Ponipodef12, which was isolated from the hybrid 'Neva' of Populus deltoides Marsh × P. nigra L. using a combination of high-speed counter-current chromatography (HSCCC) and semi-preparative HPLC. Botrallin (1) with 74.73% of purity and TMC-264 (2) with 82.29% of purity were obtained through HSCCC by employing a solvent system containing n-hexane-ethyl acetate-methanol-water at a volume ratio of 1.2:1.0:0.9:1.0. It was the first time for TMC-264 (2) to be isolated from this fungus. TMC-264 (2) showed strong antimicrobial and antinematodal activity, and botrallin (1) exhibited moderate inhibitory activity on acetylcholinesterase.
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Mao
authors.forename: Ruiya
authors.initials: R
authors.lastname: Luo
authors.forename: Haiyu
authors.initials: H
authors.lastname: Luo
authors.forename: Jin
authors.initials: J
authors.lastname: Tian
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yue
authors.forename: Mingan
authors.initials: M
authors.lastname: Wang
authors.forename: Youliang
authors.initials: Y
authors.lastname: Peng
authors.forename: Ligang
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000871:Anthelmintics / Q000302:isolation & purification / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry* / Q000302:isolation & purification; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D002853:Chromatography, Liquid / Q000379:methods*; D060026:Endophytes / Q000737:chemistry / Q000302:isolation & purification; D006575:Heterocyclic Compounds, 3-Ring / Q000302:isolation & purification* / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D032107:Populus / Q000382:microbiology
chemical_list: D000871:Anthelmintics; D000900:Anti-Bacterial Agents; D002800:Cholinesterase Inhibitors; D006575:Heterocyclic Compounds, 3-Ring