title: Transfection of exogenous rotavirus rearranged RNA segments in cells infected with a WT rotavirus results in subsequent gene rearrangements.
abstract: Group A rotaviruses, members of the family Reoviridae, are a major cause of infantile acute gastroenteritis. The rotavirus genome consists of 11 dsRNA segments. In some cases, an RNA segment is replaced by a rearranged RNA segment, which is derived from its standard counterpart by partial sequence duplication. It has been shown that some rearranged segments are preferentially encapsidated into viral progenies after serial passages in cell culture. Based on this characteristic, a reverse genetics system was used previously to introduce exogenous segment 7 rearrangements into an infectious rotavirus. This study extends this reverse genetics system to RNA segments 5 and 11. Transfection of exogenous rotavirus rearranged RNA segment 5 or 11 into cells infected with a WT helper rotavirus (bovine strain RF) resulted in subsequent gene rearrangements in the viral progeny. Whilst recombinant viruses were rescued with an exogenous rearranged segment 11, the exogenous segment was modified by a secondary rearrangement. The occurrence of spontaneous rearrangements of WT or exogenous segments is a major hindrance to the use of this reverse genetics approach.
authors.affiliation: ERL U1157/UMR 7203, Institut National de la Santé et de la Recherche Médicale, Paris, France.|Micro-Organismes, Molécules Bioactives et Physiopathologie Intestinale, Université Pierre et Marie Curie, Paris 6, Paris, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Duponchel
authors.affiliation: ERL U1157/UMR 7203, Institut National de la Santé et de la Recherche Médicale, Paris, France.|Micro-Organismes, Molécules Bioactives et Physiopathologie Intestinale, Université Pierre et Marie Curie, Paris 6, Paris, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Troupin
authors.affiliation: ERL U1157/UMR 7203, Institut National de la Santé et de la Recherche Médicale, Paris, France.|Micro-Organismes, Molécules Bioactives et Physiopathologie Intestinale, Université Pierre et Marie Curie, Paris 6, Paris, France.
authors.forename: Lan Trang
authors.initials: LT
authors.lastname: Vu
authors.affiliation: Laboratoire de Virologie, Hôpital Armand Trousseau, Assistance Publique - Hôpitaux de Paris, Paris, France.|ERL U1157/UMR 7203, Institut National de la Santé et de la Recherche Médicale, Paris, France.|Micro-Organismes, Molécules Bioactives et Physiopathologie Intestinale, Université Pierre et Marie Curie, Paris 6, Paris, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Schnuriger
authors.affiliation: ERL U1157/UMR 7203, Institut National de la Santé et de la Recherche Médicale, Paris, France.|Micro-Organismes, Molécules Bioactives et Physiopathologie Intestinale, Université Pierre et Marie Curie, Paris 6, Paris, France.
authors.forename: Germain
authors.initials: G
authors.lastname: Trugnan
authors.affiliation: Laboratoire de Virologie, Hôpital Armand Trousseau, Assistance Publique - Hôpitaux de Paris, Paris, France.|ERL U1157/UMR 7203, Institut National de la Santé et de la Recherche Médicale, Paris, France.|Micro-Organismes, Molécules Bioactives et Physiopathologie Intestinale, Université Pierre et Marie Curie, Paris 6, Paris, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Garbarg-Chenon
mesh_terms: D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002460:Cell Line; D002645:Chickens; D002522:Chlorocebus aethiops; D015321:Gene Rearrangement / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012401:Rotavirus / Q000235:genetics* / Q000472:pathogenicity; D012400:Rotavirus Infections / Q000821:virology; D017423:Sequence Analysis, RNA; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; C104282:nsp1 protein, Rotavirus