title: The role of molecular biology in the diagnosis of lymphoid neoplasms.
abstract: In recent years, DNA-arrays, gene expression profiling and next-generation sequencing have elucidated the high complexity of genomic alterations occurring in lymphoid malignancies. These studies have also contributed to the identification of new diagnostic and prognostic biomarkers, which may represent possible targets for new therapeutic approaches. Such recent advances have significantly expanded the application of molecular tests to routine diagnostic hematopathology. It is thus conceivable that next-generation assays will soon flank traditional clonality tests and chromosomal translocation assays in the diagnostic work-up of difficult cases. This review is focused on the application of molecular biology techniques in the study of lymphoid tumors. Both conventional and next-generation tests will be addressed, with particular attention to their application to clinical practice.
authors.affiliation: Department of Medicine, DIMED, General Pathology,Cytopathology Unit, University of Padova, Padova, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Pizzi
authors.affiliation: Department of Medicine, DIMED, General Pathology,Cytopathology Unit, University of Padova, Padova, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Gazzola
authors.affiliation: Department of Medicine, DIMED, General Pathology,Cytopathology Unit, University of Padova, Padova, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Mannu
authors.affiliation: Department of Medicine, DIMED, General Pathology,Cytopathology Unit, University of Padova, Padova, Italy.
authors.forename: Stefano A
authors.initials: SA
authors.lastname: Pileri
authors.affiliation: Department of Medicine, DIMED, General Pathology,Cytopathology Unit, University of Padova, Padova, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Sabattini
authors.affiliation: Department of Medicine, DIMED, General Pathology,Cytopathology Unit, University of Padova, Padova, Italy.
authors.forename: Stefano A
authors.initials: SA
authors.lastname: Pileri
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D002869:Chromosome Aberrations; D020869:Gene Expression Profiling / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008223:Lymphoma / Q000175:diagnosis* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011379:Prognosis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor