title: The plant glycosyltransferase clone collection for functional genomics.
abstract: The glycosyltransferases (GTs) are an important and functionally diverse family of enzymes involved in glycan and glycoside biosynthesis. Plants have evolved large families of GTs which undertake the array of glycosylation reactions that occur during plant development and growth. Based on the Carbohydrate-Active enZymes (CAZy) database, the genome of the reference plant Arabidopsis thaliana codes for over 450 GTs, while the rice genome (Oryza sativa) contains over 600 members. Collectively, GTs from these reference plants can be classified into over 40 distinct GT families. Although these enzymes are involved in many important plant specific processes such as cell-wall and secondary metabolite biosynthesis, few have been functionally characterized. We have sought to develop a plant GTs clone resource that will enable functional genomic approaches to be undertaken by the plant research community. In total, 403 (88%) of CAZy defined Arabidopsis GTs have been cloned, while 96 (15%) of the GTs coded by rice have been cloned. The collection resulted in the update of a number of Arabidopsis GT gene models. The clones represent full-length coding sequences without termination codons and are Gateway® compatible. To demonstrate the utility of this JBEI GT Collection, a set of efficient particle bombardment plasmids (pBullet) was also constructed with markers for the endomembrane. The utility of the pBullet collection was demonstrated by localizing all members of the Arabidopsis GT14 family to the Golgi apparatus or the endoplasmic reticulum (ER). Updates to these resources are available at the JBEI GT Collection website http://www.addgene.org/.
authors.affiliation: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
authors.forename: Jeemeng
authors.initials: J
authors.lastname: Lao
authors.forename: Ai
authors.initials: A
authors.lastname: Oikawa
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Bromley
authors.forename: Peter
authors.initials: P
authors.lastname: McInerney
authors.forename: Anongpat
authors.initials: A
authors.lastname: Suttangkakul
authors.forename: Andreia M
authors.initials: AM
authors.lastname: Smith-Moritz
authors.forename: Hector
authors.initials: H
authors.lastname: Plahar
authors.forename: Tsan-Yu
authors.initials: TY
authors.lastname: Chiu
authors.forename: Susana M
authors.initials: SM
authors.lastname: González Fernández-Niño
authors.forename: Berit
authors.initials: B
authors.lastname: Ebert
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Katy M
authors.initials: KM
authors.lastname: Christiansen
authors.forename: Sara F
authors.initials: SF
authors.lastname: Hansen
authors.forename: Solomon
authors.initials: S
authors.lastname: Stonebloom
authors.forename: Paul D
authors.initials: PD
authors.lastname: Adams
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Ronald
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Hillson
authors.forename: Masood Z
authors.initials: MZ
authors.lastname: Hadi
authors.forename: Miguel E
authors.initials: ME
authors.lastname: Vega-Sánchez
authors.forename: Dominique
authors.initials: D
authors.lastname: Loqué
authors.forename: Henrik V
authors.initials: HV
authors.lastname: Scheller
authors.forename: Joshua L
authors.initials: JL
authors.lastname: Heazlewood
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D023281:Genomics; D016695:Glycosyltransferases / Q000378:metabolism*
chemical_list: D016695:Glycosyltransferases
keywords: Arabidopsis; GT14; biolistics; cell wall; endomembrane; glycosyltransferase; particle bombardment; rice; subcellular localization