title: Surface-assisted large-scale ordering of DNA origami tiles.
abstract: The arrangement of DNA-based nanostructures into extended higher order assemblies is an important step towards their utilization as functional molecular materials. We herein demonstrate that by electrostatically controlling the adhesion and mobility of DNA origami structures on mica surfaces by the simple addition of monovalent cations, large ordered 2D arrays of origami tiles can be generated. The lattices can be formed either by close-packing of symmetric, non-interacting DNA origami structures, or by utilizing blunt-end stacking interactions between the origami units. The resulting crystalline lattices can be readily utilized as templates for the ordered arrangement of proteins.
authors.affiliation: Physik-Department E14 and ZNN/WSI, TU München, Am Coulombwall 4a, 85748 Garching (Germany).
authors.forename: Ali
authors.initials: A
authors.lastname: Aghebat Rafat
authors.forename: Tobias
authors.initials: T
authors.lastname: Pirzer
authors.forename: Max B
authors.initials: MB
authors.lastname: Scheible
authors.forename: Anna
authors.initials: A
authors.lastname: Kostina
authors.forename: Friedrich C
authors.initials: FC
authors.lastname: Simmel
mesh_terms: D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation; D055672:Static Electricity
chemical_list: D004247:DNA
keywords: DNA nanotechnology; DNA origami; self-assembly