title: Development of an ultraperformance liquid chromatography-tandem mass spectrometry method for the analysis of perfluorinated compounds in fish and Fatty food.
abstract: This paper reports the development of a method for the quantitative analysis of perfluorinated compounds (PFCs), including C6-C14 perfluorinated carboxylic acids (PFCAs) and C4-C12 perfluorinated sulfonates (PFSAs), in fish and fatty foods by ultraperformance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) in which the UPLC was equipped a PFC Analysis Kit to eliminate background contamination. Rapid baseline separation was achieved for 17 PFCs within 12 min, and PFCs were well-resolved from potential interferences from taurodeoxycholic acid and branched isomers of PFCs. The method was validated according to Commission Regulation 2002/657/EC of the European Commission with matrices including salmon, beef, egg, and butter. Average spiked recoveries, measured at concentration levels of 0.02 (method limit of quantification (MLOQ)), 0.2, and 2 μg/kg, were in the range of 68-117% with relative standard deviations below 20%. Matrix effects were evaluated and found to be correctable by internal standardization, especially for short- and long-chained PFCs. Trueness was verified against two certified reference materials. The method has also been successfully applied to the analysis of more than 200 food samples of a risk assessment study.
authors.affiliation: Food Research Laboratory, Food and Environmental Hygiene Department, 4/F Public Health Laboratory Centre, 382 Nam Cheong Street, Hong Kong SAR, China.
authors.forename: Stephen W C
authors.initials: SW
authors.lastname: Chung
authors.forename: Chi Ho
authors.initials: CH
authors.lastname: Lam
mesh_terms: D000818:Animals; D002079:Butter / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006845:Hydrocarbons, Fluorinated; D002079:Butter