title: Dysregulated microRNA clusters in response to retinoic acid and CYP26B1 inhibitor induced testicular function in dogs.
abstract: Spermatogenesis is a multistep synchronized process. Diploid spermatogonia differentiate into haploid spermatozoa following mitosis, meiosis and spermiogenesis. Division and differentiation of male germ cells is achieved through the sequential expression of several genes. Numerous mRNAs in the differentiating germ cells undergo post-transcriptional and translational regulation. MiRNAs are powerful negative regulators of mRNA transcription, stability, and translation and recognize their mRNA targets through base-pairing. Retinoic acid (RA) signaling is essential for spermatogenesis and testicular function. Testicular RA level is critical for RA signal transduction. This study investigated the miRNAs modulation in an RA- induced testicular environment following the administration of all-trans RA (2 µM) and CYP26B1- inhibitor (1 µM) compared to control. Eighty four canine mature miRNAs were analyzed and their expression signatures were distinguished using real-time PCR based array technology. Of the miRNAs analyzed, miRNA families such as miR-200 (cfa-miR-200a, cfa-miR-200b and cfa-miR-200c), Mirlet-7 (cfa-let-7a, cfa-let-7b, cfa-let-7c, cfa-let-7g and cfa-let-7f), miR-125 (cfa-miR-125a and cfa-miR-125b), miR-146 (cfa-miR-146a and cfa-miR-146b), miR-34 (cfa-miR-34a, cfa-miR-34b and cfa-miR-34c), miR-23 (cfa-miR-23a and cfa-miR-23b), cfa-miR-184, cfa-miR-214 and cfa-miR-141 were significantly up-regulated with testicular RA intervention via administration of CYP26B1 inhibitor and all-trans-RA and species of miRNA such as cfa-miR-19a, cfa-miR-29b, cfa-miR-29c, cfa-miR-101 and cfa-miR-137 were significantly down-regulated. This study explored information regarding chromosome distribution, human orthologous sequences and the interaction of target genes of miRNA families significantly distinguished in this study using prediction algorithms. This study importantly identified dysregulated miRNA species resulting from RA-induced spermatogenesis. The present contribution serves as a useful resource for further elucidation of the regulatory role of individual miRNA in RA synchronized canine spermatogenesis.
authors.affiliation: Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington, United States of America.
authors.forename: Vanmathy R
authors.initials: VR
authors.lastname: Kasimanickam
authors.affiliation: Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington, United States of America.
authors.forename: Ramanathan K
authors.initials: RK
authors.lastname: Kasimanickam
authors.affiliation: Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington, United States of America.
authors.forename: William S
authors.initials: WS
authors.lastname: Dernell
mesh_terms: D000818:Animals; D052160:Benzothiazoles / Q000494:pharmacology; D016000:Cluster Analysis; D065607:Cytochrome P-450 Enzyme Inhibitors / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004285:Dogs; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D053263:Gene Regulatory Networks; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D005810:Multigene Family; D000072516:Retinoic Acid 4-Hydroxylase; D013737:Testis / Q000187:drug effects* / Q000378:metabolism*; D059467:Transcriptome; D014212:Tretinoin / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology
chemical_list: D052160:Benzothiazoles; D065607:Cytochrome P-450 Enzyme Inhibitors; D035683:MicroRNAs; D014230:Triazoles; D014212:Tretinoin; D003577:Cytochrome P-450 Enzyme System; D000072516:Retinoic Acid 4-Hydroxylase; C406527:R 115866