title: Effectiveness of interleukin-1 receptor antagonist (Anakinra) on cerulein-induced experimental acute pancreatitis in rats.
abstract: OBJECTIVE
  Acute pancreatitis (AP) is defined as an inflammatory disease of the pancreas. The purpose of this study was to examine the effectiveness of Anakinra on cerulein-induced experimental pancreatitis rat model by using the results of biochemical and histopathological findings.
  
  
  METHODS
  Cerulein was administered to induce AP in rats. Group 1 was the sham group. Subcutancerulein was injected to the rats in group 2 for experimental pancreatitis group. In groups 3 and 4, 100 and 50 mg/kg intraperitoneal Anakinra were injected after the induction of experimental pancreatitis by subcutaneous cerulein in rats, respectively. Lastly, in group 5, rats were injected with intraperitoneal saline and subcutan cerulean for placebo group. The following parameters were evaluated: histopathological score of pancreatitis, apoptotic index, amylase, lipase, TNF-α levels, IL-1β and the leukocyte count.
  
  
  RESULTS
  When the results of serum amylase, lipase, TNF-α and IL-1β levels, the leukocyte count, histopathologic scores and apoptotic indices of control group compared to the results of other groups, the differences exhibited statistical significance (all p < 0.05). On the other hand, when the results of fourth group compared with the results of third group, the data demonstrated statistical insignificance (p > 0.05). However, no any significant differences were found between the results of fourth and fifth groups (p > 0.05).
  
  
  CONCLUSIONS
  In the light of these results, cerulein is an appropriate agent for experimental AP rat model and Anakinra has a favorable therapeutic effect on acute experimental pancreatitis model. Moreover, Anakinra significantly decreases cerulein-related pancreatic tissue injury and pancreatic apoptosis.
authors.affiliation: Department of Gastroenterology, GATA Haydarpasa Training Hospital , Uskudar-Istanbul , Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Kaplan
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Yazgan
authors.forename: Alpaslan
authors.initials: A
authors.lastname: Tanoglu
authors.forename: Ufuk
authors.initials: U
authors.lastname: Berber
authors.forename: Kemal
authors.initials: K
authors.lastname: Oncu
authors.forename: Muammer
authors.initials: M
authors.lastname: Kara
authors.forename: Dilaver
authors.initials: D
authors.lastname: Demirel
authors.forename: Irfan
authors.initials: I
authors.lastname: Kucuk
authors.forename: Halil Onur
authors.initials: HO
authors.lastname: Ozari
authors.forename: Osman Metin
authors.initials: OM
authors.lastname: Ipcioglu
mesh_terms: D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D002108:Ceruletide; D004195:Disease Models, Animal; D053590:Interleukin 1 Receptor Antagonist Protein / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000097:blood; D007958:Leukocyte Count; D008049:Lipase / Q000097:blood; D008297:Male; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D002108:Ceruletide; D008049:Lipase; D000681:Amylases
keywords: Anakinra; acute pancreatitis; cerulein; inflammation