title: Preparative isolation and purification of iridoid glycosides from Fructus Corni by high-speed countercurrent chromatography.
abstract: Using a two-phase solvent system composed of dichloromethane-methanol-n-butanol-water-acetic acid (5:5:3:4:0.1,v/v/v/v/v), high-speed countercurrent chromatography was successfully performed for isolation and purification of three iridoid glycosides from Fructus Corni for the first time. From 100 mg of a crude extract of Fructus Corni 7.9 mg of sweroside, 13.1 mg of morroniside, and 10.2 mg of loganin were obtained in less than 3 h with purities of 92.3, 96.3 and 94.2%, respectively. These target compounds were identified by ESI-MS,1H NMR and13C NMR.
authors.affiliation: Biomedicine Key Laboratory of shaanxi province, Northwest University, Xi'an 710069, China.
authors.forename: Jinru
authors.initials: J
authors.lastname: Liang
authors.affiliation: Biomedicine Key Laboratory of shaanxi province, Northwest University, Xi'an 710069, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: He
authors.affiliation: Biomedicine Key Laboratory of shaanxi province, Northwest University, Xi'an 710069, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Biomedicine Key Laboratory of shaanxi province, Northwest University, Xi'an 710069, China.
authors.forename: Wenna
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Institut Parisien de Chimie Moléculaire, Université Pierre et Marie Curie-Paris 6 (UMR CNRS 7201), 4 place Jussieu, 75005 Paris, France.
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Biomedicine Key Laboratory of shaanxi province, Northwest University, Xi'an 710069, China.
authors.forename: Wenji
authors.initials: W
authors.lastname: Sun
keywords: Fructus Corni; high-speed countercurrent chromatography; iridoid glycosides