title: Fabrication of cell penetration enhanced poly (l-lactic acid-co-ɛ-caprolactone)/silk vascular scaffolds utilizing air-impedance electrospinning.
abstract: In the vascular prosthetic field, the prevailing thought is that for clinical, long-term success, especially bioresorbable grafts, cellular migration and penetration into the prosthetic structure is required to promote neointima formation and vascular wall development. In this study, we fabricated poly (l-lactic acid-co-ɛ-caprolactone) P(LLA-CL)/silk fibroin (SF) vascular scaffolds through electrospinning using both perforated mandrel subjected to various intraluminal air pressures (0-300kPa), and solid mandrel. The scaffolds were evaluated the cellular infiltration in vitro and mechanical properties. Vascular scaffolds were seeded with smooth muscle cells (SMCs) to evaluate cellular infiltration at 1, 7, and 14 days. The results revealed that air-impedance scaffolds allowed significantly more cell infiltration as compared to the scaffolds fabricated with solid mandrel. Meanwhile, results showed that both mandrel model and applied air pressure determined the interfiber distance and the alignment of fibers in the enhanced porosity regions of the structure which influenced cell infiltration. Uniaxial tensile testing indicated that the air-impedance scaffolds have sufficient ultimate strength, suture retention strength, and burst pressure as well as compliance approximating a native artery. In conclusion, the air-impedance scaffolds improved cellular infiltration without compromising overall biomechanical properties. These results support the scaffold's potential for vascular grafting and in situ regeneration.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Anlin
authors.initials: A
authors.lastname: Yin
authors.affiliation: College of Architecture and Environment, Sichuan University, Chengdu 610065, China.
authors.forename: Jiukai
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, University of Memphis, Memphis, Tennessee, TN 38152, USA.
authors.forename: Gary L
authors.initials: GL
authors.lastname: Bowlin
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, University of Memphis, Memphis, Tennessee, TN 38152, USA.
authors.forename: Isaac A
authors.initials: IA
authors.lastname: Rodriguez
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt.
authors.forename: Hany A
authors.initials: HA
authors.lastname: Ei-Hamshary
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Salem S
authors.initials: SS
authors.lastname: Al-Deyab
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address: xmm@dhu.edu.cn.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
mesh_terms: D000388:Air; D000818:Animals; D016880:Anisotropy; D001807:Blood Vessel Prosthesis; D012831:Bombyx; D002463:Cell Membrane Permeability / Q000187:drug effects*; D017097:Electric Impedance; D005583:Fourier Analysis; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects; D011091:Polyesters / Q000494:pharmacology*; D011312:Pressure; D047011:Silk / Q000494:pharmacology*; D013718:Tensile Strength / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; D047011:Silk; C550605:poly(lactic acid-co-epsilon-caprolactone)
keywords: Cell infiltration; Electrospinning; Vascular prosthetic; Vascular tissue engineering