title: A comparative study of the effects of growth and differentiation factor 5 on muscle-derived stem cells and bone marrow stromal cells in an in vitro tendon healing model.
abstract: OBJECTIVE
  To investigate the ability of muscle-derived stem cells (MDSCs) supplemented with growth and differentiation factor-5 (GDF-5) to improve tendon healing compared with bone marrow stromal cells (BMSCs) in an in vitro tendon culture model.
  
  
  METHODS
  Eighty canine flexor digitorum profundus tendons were assigned into 5 groups: repaired tendon (1) without gel patch interposition (no cell group), (2) with BMSC-seeded gel patch interposition (BMSC group), (3) with MDSC-seeded gel patch interposition (MDSC group), (4) with GDF-5-treated BMSC-seeded gel patch interposition (BMSC+GDF-5 group), and (5) with GDF-5-treated MDSC-seeded gel patch interposition (MDSC+GDF-5 group). After culturing for 2 or 4 weeks, the failure strength of the healing tendons was measured. The tendons were also evaluated histologically.
  
  
  RESULTS
  The failure strength of the repaired tendon in the MDSC+GDF-5 group was significantly higher than that of the non-cell and BMSC groups. The stiffness of the repaired tendons in the MDSC+GDF-5 group was significantly higher than that of the non-cell group. Histologically, the implanted cells became incorporated into the original tendon in all 4 cell-seeded groups.
  
  
  CONCLUSIONS
  Interposition of a multilayered GDF-5 and MDSC-seeded collagen gel patch at the repair site enhanced tendon healing compared with a similar patch using BMSC. However, this increase in vitro was relatively small. In the clinical setting, differences between MDSC and BMSC may not be substantially different, and it remains to be shown that such methods might enhance the results of an uncomplicated tendon repair clinically.
  
  
  CONCLUSIONS
  Muscle-derived stem cell implantation and administration of GDF-5 may improve the outcome of tendon repair.
authors.affiliation: Division of Orthopedic Research and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ozasa
authors.affiliation: Division of Orthopedic Research and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN.
authors.forename: Anne
authors.initials: A
authors.lastname: Gingery
authors.affiliation: Division of Orthopedic Research and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Thoreson
authors.affiliation: Division of Orthopedic Research and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN.
authors.forename: Kai-Nan
authors.initials: KN
authors.lastname: An
authors.affiliation: Division of Orthopedic Research and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Division of Orthopedic Research and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN. Electronic address: pamadio@mayo.edu.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Amadio
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D004285:Dogs; D055428:Growth Differentiation Factor 5 / Q000494:pharmacology*; D066298:In Vitro Techniques; D045164:Mesenchymal Stem Cell Transplantation; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000166:cytology*; D013708:Tendon Injuries / Q000628:therapy*; D046509:Tissue Culture Techniques; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D055428:Growth Differentiation Factor 5
keywords: Muscle-derived stem cell; bone marrow stromal cell; flexor tendon repair; growth differentiation factor-5