title: Antibody secreting cell assay for influenza A virus in swine.
abstract: An ELISPOT assay to enumerate B-cells producing antibodies specific to a given antigen, also known as an antibody secreting cell (ASC) assay, was adapted to detect B-cells specific for influenza A virus (IAV). The assay is performed ex vivo and enumerates ASC at a single cell level. A simple ASC detection method is based on a solid phase immunoenzymatic principle. The ELISPOT plate is coated with IAV prior to incubation with cell samples. The spot-forming cells are detected by enzyme labeled antibodies directed to the swine immunoglobulin (Ig) class of interest and visualized with the addition of substrate.
authors.affiliation: Virus and Prion Research Unit, National Animal Disease Center, U.S. Department of Agriculture, Agricultural Research Service, 1920 Dayton Avenue, Ames, IA, 50010, USA.
authors.forename: Pravina
authors.initials: P
authors.lastname: Kitikoon
authors.forename: Crystal L
authors.initials: CL
authors.lastname: Loving
authors.forename: Amy L
authors.initials: AL
authors.lastname: Vincent
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D002452:Cell Count / Q000379:methods*; D002469:Cell Separation; D009980:Influenza A virus / Q000276:immunology*; D013552:Swine
chemical_list: D000914:Antibodies, Viral