title: Thermoresponsive ketoprofen-imprinted monolith prepared in ionic liquid.
abstract: A thermoresponsive imprinted monolith with the ability of molecular recognition for ketoprofen was prepared for the first time. The smart monolith was synthesized in a stainless steel column using acrylamide (AAm) and 2-acrylamide-2-methyl propanesulfonic acid (AMPS) as functional monomers, which can form interpolymer complexation to restrict access of the analyte to the imprinted networks at low temperatures. To avoid a high back pressure of the column derived from neat dimethyl sulfoxide (DMSO) as a porogenic solvent that is needed to solve polar AMPS, an ionic liquid, [BMIM]BF4, was introduced into the pre-polymerization mixture. The molecular recognition ability towards ketoprofen of the resulting thermoresponsive molecularly imprinted polymer (MIP) monolith displayed significant dependence on temperature compared with a non-imprinted column (NIP), and the greatest imprinting factor was achieved at the transition temperature of 35 °C (above 10). Furthermore, the number of binding sites of the smart MIP monolith at 35 °C was about 76 times as large as that at 25 °C. In addition, Freundlich analyses indicated that the thermoresponsive MIP monolith had homogeneous affinity sites at both 25 and 35 °C with heterogeneity index 0.9251 and 0.9851, respectively.
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Sun
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Zhao
authors.forename: Xian-Hua
authors.initials: XH
authors.lastname: Wang
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Huang
authors.forename: Zhao-Sheng
authors.initials: ZS
authors.lastname: Liu
mesh_terms: D020106:Acrylamide / Q000032:analysis* / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D002626:Chemistry, Pharmaceutical; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D005506:Food Contamination; D005740:Gases; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry*; D007660:Ketoprofen / Q000737:chemistry*; D008628:Mercury; D008892:Milk / Q000737:chemistry; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D011312:Pressure; D012997:Solvents / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000032:analysis* / Q000737:chemistry; D013696:Temperature
chemical_list: D005740:Gases; D052578:Ionic Liquids; D011108:Polymers; D012997:Solvents; D013451:Sulfonic Acids; D020106:Acrylamide; D007660:Ketoprofen; D008628:Mercury; D004121:Dimethyl Sulfoxide