title: Probiotic assessment of Enterococcus durans 6HL and Lactococcus lactis 2HL isolated from vaginal microflora.
abstract: Forty-five lactic acid bacteria (LAB) were isolated from the vaginal specimens of healthy fertile women, and the identities of the bacteria were confirmed by sequencing of their 16S rDNA genes. Among these bacteria, only four isolates were able to resist and survive in low pH, bile salts and simulated in vitro digestion conditions. Lactococcus lactis 2HL, Enterococcus durans 6HL, Lactobacillus acidophilus 36YL and Lactobacillus plantarum 5BL showed the best resistance to these conditions. These strains were evaluated further to assess their ability to adhere to human intestinal Caco-2 cells. Lactococcus lactis 2HL and E. durans 6HL were the most adherent strains. In vitro tests under neutralized pH proved the antimicrobial activity of both strains. Results revealed that the growth of Escherichia coli O26, Staphylococcus aureus and Shigella flexneri was suppressed by both LAB strains. The antibiotic susceptibility tests showed that these strains were sensitive to all nine antibiotics: vancomycin, tetracycline, ampicillin, penicillin, gentamicin, erythromycin, clindamycin, sulfamethoxazole and chloramphenicol. These data suggest that E. durans 6HL and Lactococcus lactis 2HL could be examined further for their useful properties and could be developed as new probiotics.
authors.affiliation: Institute of Biosciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Yousef
authors.initials: Y
authors.lastname: Nami
authors.affiliation: Chemical and Environmental Engineering Department, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Norhafizah
authors.initials: N
authors.lastname: Abdullah
authors.affiliation: Institute of Biosciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Babak
authors.initials: B
authors.lastname: Haghshenas
authors.affiliation: Chemical and Environmental Engineering Department, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Dayang
authors.initials: D
authors.lastname: Radiah
authors.affiliation: Institute of Biosciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Rozita
authors.initials: R
authors.lastname: Rosli
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.|Biotechnology Research Center, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ahmad Yari
authors.initials: AY
authors.lastname: Khosroushahi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D024881:Drug Resistance, Bacterial; D016983:Enterococcus / Q000145:classification* / Q000187:drug effects / Q000502:physiology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017045:Lactococcus / Q000145:classification* / Q000187:drug effects / Q000502:physiology; D019936:Probiotics; D014621:Vagina / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents