title: ESNATS conference - the use of human embryonic stem cells for novel toxicity testing approaches.
abstract: The main achievements and results of the ESNATS project (Embryonic Stem Cell-based Novel Alternative Testing Strategies) were presented at the final project conference that was held on 15 September 2013, the day before the traditional EUSAAT (European Society for Alternatives to Animal Testing) Congress in Linz, Austria. The ESNATS project was an FP7 European Integrated Project, running from 2008 to 2013, the aim of which was to develop a novel toxicity testing platform based on embryonic stem cells (ESCs), and in particular, human ESC (hESCs), to accelerate drug development, reduce related R&D costs, and propose a powerful alternative to animal tests in the spirit of the Three Rs principles. Altogether, ESNATS offered the first proof of concept that hESCs can be used to create robust, reproducible and ready-to-use test assays for predicting human toxicity. In the end, essentially five test systems were developed to an adequate level for entering possible pre-validation procedures. These methods are based on hESCs, and can be combined to study the possible effects, on the human embryo, of exposure to a chemical during the early stages of development. In addition to the presentations by the main project partners, external speakers were invited to give lectures on relevant topics, both in the field of neurotoxicity and, more generally, on the applicability of hESCs in the development of advanced in vitro tests.
authors.affiliation: Centre for Alternatives to Animal Testing-Europe (CAAT-Europe), University of Konstanz, Konstanz, Germany.
authors.forename: Costanza
authors.initials: C
authors.lastname: Rovida
authors.affiliation: Department of Toxicology, Pharmacognosy and Dermato-cosmetology, Vrije Universiteit Brussel, Jette, Belgium.
authors.forename: Manon
authors.initials: M
authors.lastname: Vivier
authors.affiliation: BIO.NRW, Cluster Biotechnology North Rhine-Westphalia, Germany.
authors.forename: Bernward
authors.initials: B
authors.lastname: Garthoff
authors.affiliation: Universitätsklinikum, University of Cologne, Cologne, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hescheler
mesh_terms: D000826:Animal Testing Alternatives; D000818:Animals; D001921:Brain / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D013792:Thalidomide / Q000633:toxicity; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D013792:Thalidomide