title: Physical exercise reduces the expression of RANTES and its CCR5 receptor in the adipose tissue of obese humans.
abstract: RANTES and its CCR5 receptor trigger inflammation and its progression to insulin resistance in obese. In the present study, we investigated for the first time the effect of physical exercise on the expression of RANTES and CCR5 in obese humans. Fifty-seven adult nondiabetic subjects (17 lean and 40 obese) were enrolled in a 3-month supervised physical exercise. RANTES and CCR5 expressions were measured in PBMCs and subcutaneous adipose tissue before and after exercise. Circulating plasma levels of RANTES were also investigated. There was a significant increase in RANTES and CCR5 expression in the subcutaneous adipose tissue of obese compared to lean. In PBMCs, however, while the levels of RANTES mRNA and protein were comparable between both groups, CCR5 mRNA was downregulated in obese subjects (P < 0.05). Physical exercise significantly reduced the expression of both RANTES and CCR5 (P < 0.05) in the adipose tissue of obese individuals with a concomitant decrease in the levels of the inflammatory markers TNF- α , IL-6, and P-JNK. Circulating RANTES correlated negatively with anti-inflammatory IL-1 ra (P = 0.001) and positively with proinflammatory IP-10 and TBARS levels (P < 0.05). Therefore, physical exercise may provide an effective approach for combating the deleterious effects associated with obesity through RANTES signaling in the adipose tissue.
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait ; Queensland Children's Medical Research Institute, University of Queensland, Brisbane, QLD 4029, Australia.
authors.forename: Engin
authors.initials: E
authors.lastname: Baturcam
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Jehad
authors.identifier: 0000-0003-0681-7305
authors.initials: J
authors.lastname: Abubaker
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Ali
authors.initials: A
authors.lastname: Tiss
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Mohamed
authors.identifier: 0000-0001-8357-1252
authors.initials: M
authors.lastname: Abu-Farha
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Abdelkrim
authors.initials: A
authors.lastname: Khadir
authors.affiliation: Fitness and Rehabilitation Centre, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Fahad
authors.initials: F
authors.lastname: Al-Ghimlas
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Irina
authors.initials: I
authors.lastname: Al-Khairi
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Preethi
authors.initials: P
authors.lastname: Cherian
authors.affiliation: Department of Biostatistics and Epidemiology, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Naser
authors.initials: N
authors.lastname: Elkum
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Maha
authors.identifier: 0000-0002-8974-8255
authors.initials: M
authors.lastname: Hammad
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Jeena
authors.initials: J
authors.lastname: John
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Sina
authors.initials: S
authors.lastname: Kavalakatt
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Lehe
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Samia
authors.initials: S
authors.lastname: Warsame
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait ; Fitness and Rehabilitation Centre, Dasman Diabetes Institute, 1180 Dasman, Kuwait ; Department of Biostatistics and Epidemiology, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Kazem
authors.initials: K
authors.lastname: Behbehani
authors.affiliation: King Fahad Specialist Hospital, Dammam 15215, Saudi Arabia.
authors.forename: Said
authors.initials: S
authors.lastname: Dermime
authors.affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait ; Diabetes Research Center, Qatar Biomedical Research Institute, Education City, P.O. Box 5825, Doha, Qatar.
authors.forename: Mohammed
authors.identifier: 0000-0002-1808-2564
authors.initials: M
authors.lastname: Dehbi
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D000886:Anthropometry; D015992:Body Mass Index; D001835:Body Weight; D018946:Chemokine CCL5 / Q000097:blood*; D054357:Chemokine CXCL10 / Q000097:blood; D015444:Exercise; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D020935:MAP Kinase Signaling System; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D019713:Receptors, CCR5 / Q000097:blood*; D015398:Signal Transduction; D017392:Thiobarbituric Acid Reactive Substances; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: C483024:CCL5 protein, human; C584933:CCR5 protein, human; C114753:CXCL10 protein, human; D018946:Chemokine CCL5; D054357:Chemokine CXCL10; D015850:Interleukin-6; D012333:RNA, Messenger; D019713:Receptors, CCR5; D017392:Thiobarbituric Acid Reactive Substances; D014409:Tumor Necrosis Factor-alpha