title: Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro.
abstract: BACKGROUND
  Patterning medical devices at the nanoscale level enables the manipulation of cell behavior and tissue regeneration, with topographic features recognized as playing a significant role in the osseointegration of implantable devices.
  
  
  METHODS
  In this study, we assessed the ability of titanium-coated hemisphere-like topographic nanostructures of different sizes (approximately 50, 100, and 200 nm) to influence the morphology, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs).
  
  
  RESULTS
  We found that the proliferation and osteogenic differentiation of hMSCs was influenced by the size of the underlying structures, suggesting that size variations in topographic features at the nanoscale level, independently of chemistry, can be exploited to control hMSC behavior in a size-dependent fashion.
  
  
  CONCLUSIONS
  Our studies demonstrate that colloidal lithography, in combination with coating technologies, can be exploited to investigate the cell response to well defined nanoscale topography and to develop next-generation surfaces that guide tissue regeneration and promote implant integration.
authors.affiliation: The New York Stem Cell Foundation Research Institute, New York, NY, USA ; Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden ; BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Giuseppe Maria
authors.initials: GM
authors.lastname: de Peppo
authors.affiliation: Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden ; BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Hossein
authors.initials: H
authors.lastname: Agheli
authors.affiliation: Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden ; BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Camilla
authors.initials: C
authors.lastname: Karlsson
authors.affiliation: Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden ; BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Ekström
authors.affiliation: BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden ; Department of Orthopaedics, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Helena
authors.initials: H
authors.lastname: Brisby
authors.affiliation: Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden ; BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Lennerås
authors.affiliation: BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden ; Applied Physics, Chalmers University of Technology, Göteborg, Sweden.
authors.forename: Stefan
authors.initials: S
authors.lastname: Gustafsson
authors.affiliation: BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden ; Applied Physics, Chalmers University of Technology, Göteborg, Sweden ; Chemistry, Materials and Surfaces, SP Technical Research Institute of Sweden, Borås, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Sjövall
authors.affiliation: Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden ; BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Johansson
authors.affiliation: BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden ; Applied Physics, Chalmers University of Technology, Göteborg, Sweden.
authors.forename: Eva
authors.initials: E
authors.lastname: Olsson
authors.affiliation: BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden ; Chemistry, Materials and Surfaces, SP Technical Research Institute of Sweden, Borås, Sweden.
authors.forename: Jukka
authors.initials: J
authors.lastname: Lausmaa
authors.affiliation: Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden ; BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Thomsen
authors.affiliation: BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, University of Gothenburg, Göteborg, Sweden ; Chemistry, Materials and Surfaces, SP Technical Research Institute of Sweden, Borås, Sweden.
authors.forename: Sarunas
authors.initials: S
authors.lastname: Petronis
mesh_terms: D065966:Absorption, Physicochemical; D002478:Cells, Cultured; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D054802:Molecular Imprinting / Q000379:methods; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology*; D010316:Particle Size; D010781:Photography / Q000379:methods; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium / Q000737:chemistry*
chemical_list: D014025:Titanium
keywords: cell proliferation; colloidal lithography; human mesenchymal stem cells; implantable materials; mineralization; nanotopography; osteogenic differentiation