title: Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double-muscled (DM) and normal-muscled (NM) Japanese shorthorn cattle.
abstract: The purpose of this study was to determine whether myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double-muscled (DM) and normal-muscled (NM) Japanese Shorthorn cattle. Plasma concentrations of glucose were lower in DM cattle than in NM cattle (P < 0.01). The expression of GLUT4 messenger RNA (mRNA) in the skeletal muscle ex vivo and in myoblasts at 72 h after differentiation in vitro was higher in DM cattle than in NM cattle (P < 0.01). In contrast, the NM and DM cattle did not differ with respect to skeletal muscle expression of GLUT1 and myocyte enhancer factor-2c (MEF2c), a transcription factor of GLUT4. In differentiated myoblasts, the expression of GLUT1, GLUT4, and MEF2c mRNAs was greater in DM cattle than in NM cattle (P < 0.01). In the presence and absence of insulin, glucose uptake in myoblasts was increased in DM cattle relative to that of NM cattle (P < 0.01). The addition of myostatin decreased the expression of GLUT4 and MEF2c mRNAs in DM myoblasts (P < 0.05). Results of the present study suggest that myostatin inhibits the expression of GLUT4 mRNA possibly via MEF2c and that the greater ability of the DM cattle to produce muscle relative to the NM cattle may be due to their greater sensitivity to insulin and greater use of glucose.
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai-shi, Japan; Faculty of Agriculture, Graduate school, Kuju Agricultural Research Center, Kyushu University, Kuju-cho, Taketa-shi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai-shi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai-shi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.affiliation: Genome Research, Tokushima University, Kuramoto-machi, Tokushima-shi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Miyake
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai-shi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai-shi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagasawa
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai-shi, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Kitagawa
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai-shi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Terada
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai-shi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Urakawa
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Cardiganshire, SY23 3DA, UK.
authors.forename: M T
authors.initials: MT
authors.lastname: Rose
authors.affiliation: Institute for Growth Physiology Group, AgResearch Ltd, Private Bag 3123, Hamilton, New Zealand.
authors.forename: C D
authors.initials: CD
authors.lastname: McMahon
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai-shi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai-shi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ohwada
authors.affiliation: Faculty of Agriculture, Graduate school, Kuju Agricultural Research Center, Kyushu University, Kuju-cho, Taketa-shi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Gotoh
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai-shi, Japan. Electronic address: asosan@bios.tohoku.ac.jp.
authors.forename: H
authors.initials: H
authors.lastname: Aso
mesh_terms: D000818:Animals; D001786:Blood Glucose; D002417:Cattle / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D005951:Glucose Tolerance Test / Q000662:veterinary; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism*; D007328:Insulin; D018482:Muscle, Skeletal / Q000378:metabolism*; D032446:Myoblasts / Q000378:metabolism*; D055435:Myostatin / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D051275:Glucose Transporter Type 4; D007328:Insulin; D055435:Myostatin; D012333:RNA, Messenger
keywords: Double-muscled Japanese shorthorn; GLUT-1; GLUT-4; Glucose uptake; MEF2c