title: Pheromone 3kPZS evokes context-dependent serotonin sexual dimorphism in the brain of the sea lamprey (Petromyzon marinus).
abstract: Mature male sea lampreys (Petromyzon marinus) release a sex pheromone, 3-keto-petromyzonol sulfate (3kPZS), that induces sexually dimorphic behavioral responses in conspecifics. However, the neural mechanism of such responses is mostly unknown. We examined the neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) and the expression of 5-HT1A receptors in the forebrain and brainstem of sea lamprey exposed to the vehicle (0.91 ppm methanol) or 10(-10) M 3kPZS for 2 h using high performance liquid chromatography-electrospray ionization tandem mass spectrometry, immunohistochemistry and real-time quantitative polymerase chain reaction. Exposure to 3kPZS for 2 h increased 5-HT concentration in the forebrain of adult females, whereas 5-HT was not detected in the forebrain of adult males. On the contrary, 3kPZS exposure decreased 5-HT concentration in the brainstem of adult females and had no effect in adult males. Pheromone exposure evoked context-dependent sexual dimorphism in brain 5-HT1A receptor immunoreactivity, but had no effect on 5-HT1A mRNA concentrations in the brain with 2 h exposure time. It appears that in sea lamprey pheromone 3kPZS affects the 5-HT system in the brain in a context-dependent, sexually dimorphic manner.
authors.affiliation: Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI, USA.
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Chung-Davidson
authors.forename: Huiyong
authors.initials: H
authors.lastname: Wang
authors.forename: Anne M
authors.initials: AM
authors.lastname: Scott
authors.forename: Weiming
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D001933:Brain Stem / Q000187:drug effects / Q000378:metabolism; D002793:Cholic Acids / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation; D008297:Male; D048428:Petromyzon / Q000502:physiology*; D010675:Pheromones / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D044263:Receptors, Serotonin, 5-HT1; D012701:Serotonin / Q000378:metabolism*; D012727:Sex Characteristics; D053719:Tandem Mass Spectrometry
chemical_list: D002793:Cholic Acids; D010675:Pheromones; D044263:Receptors, Serotonin, 5-HT1; D012701:Serotonin; C500317:petromyzonol sulfate
keywords: 5-HT1A receptor; liquid chromatography-electrospray ionization tandem mass spectrometry; motor output; real-time quantitative polymerase chain reaction