title: A bryozoan species may offer novel antioxidants with anti-carbon-dioxide anion radical activity.
abstract: The antiradical activity of the freshwater bryozoan Hyalinella punctata water extracts (two samples, seasonal collection) was evaluated by using electron paramagnetic resonance spectroscopy against hydroxyl (√OH), superoxide anion (√O2(- )), methoxy (√CH2OH), carbon-dioxide anion (√CO2(- )), nitric-oxide (√NO) and 2,2-diphenyl-1-picrylhydrazyl (√DPPH) radicals. The extracts reduced the production of all tested radicals but to a varying degree. The better activity was observed against √CO2(- ) and √CH2OH radicals (54 ± 5% and 44 ± 4%, and 58 ± 6% and 22 ± 2%, respectively) than towards √DPPH, √NO, √OH and √O2(- ) radicals (59 ± 6% and 1.0 ± 0.1%, 46 ± 5% and 14 ± 1%, 7.0 ± 0.5% and 34 ± 3%, and 33 ± 3% and 0%, respectively). FTIR spectra of the both extracts indicate the presence of cyclic peptides and polypeptides which might be responsible for the observed activity. According to the experimental data obtained, H. punctata water extract may be considered as a novel promising resource of natural products with anti √CO2(- ) radical activity.
authors.affiliation: a Department of Life Sciences , Institute for Multidisciplinary Research - IMSI, University of Belgrade , Belgrade , Serbia.
authors.forename: Boris
authors.initials: B
authors.lastname: Pejin
authors.forename: Aleksandar G
authors.initials: AG
authors.lastname: Savic
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Hegedis
authors.forename: Ivo
authors.initials: I
authors.lastname: Karaman
authors.forename: Mladen
authors.initials: M
authors.lastname: Horvatovic
authors.forename: Milos
authors.initials: M
authors.lastname: Mojovic
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology; D002013:Bryozoa / Q000737:chemistry*; D002245:Carbon Dioxide / Q000737:chemistry / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000737:chemistry; D005618:Fresh Water; D009569:Nitric Oxide / Q000737:chemistry; D010851:Picrates / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001688:Biological Products; D001713:Biphenyl Compounds; D005609:Free Radicals; D010851:Picrates; D002245:Carbon Dioxide; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: DEPMPO; EPR; Hyalinella punctata; free radicals; water extracts