title: In vitro inhibition of Cyprinid herpesvirus-3 replication by RNAi.
abstract: Cyprinid herpesvirus-3 (CyHV-3) is an etiological agent of a notifiable disease that causes high mortality rates affecting both the common and koi carp Cyprinus carpio L. There is no current treatment strategy to save CyHV-3 infected fish. RNA mediated interference (RNAi) is an emerging strategy used for understanding gene function and is a promising method in developing novel therapeutics and antiviral medications. For this study, the possibility of activating the RNAi pathway by the use of small interfering (si)RNAs was tested to inhibit in vitro viral replication of CyHV-3 in common carp brain (CCB) cells. The siRNAs were designed to target either thymidine kinase (TK) or DNA polymerase (DP) genes, which both code for transcripts involved in DNA replication. The inhibition of viral replication caused by the siRNAs was measured by a reporter gene, termed ORF81. Treatment with siRNA targeting either TK or DP genes reduced the release of viral particles from infected CCB cells. However, siRNA targeting DP was most effective at reducing viral release as measured by qPCR.
authors.affiliation: Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Gotesman
authors.affiliation: Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria; Fish Medicine and Management, Faculty of Veterinary Medicine, University of Assiut, 71515 Assiut, Egypt.
authors.forename: Hatem
authors.initials: H
authors.lastname: Soliman
authors.affiliation: Clinic and Policlinic for Dermatology and Allergology, Department of Dermatology, Ludwig-Maximilian University, Munich, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Besch
authors.affiliation: Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria. Electronic address: Mansour.El-Matbouli@vetmeduni.ac.at.
authors.forename: Mansour
authors.initials: M
authors.lastname: El-Matbouli
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002347:Carps / Q000821:virology*; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D006564:Herpesviridae / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology*; D019562:Viral Load; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D034741:RNA, Small Interfering
keywords: CyHV-3; Inhibition; KHV; RNAi