title: The SH2 domain is crucial for function of Fyn in neuronal migration and cortical lamination.
abstract: Neurons in the developing brain form the cortical plate (CP) in an inside-out manner, in which the late-born neurons are located more superficially than the early-born neurons. Fyn, a member of the Src family kinases, plays an important role in neuronal migration by binding to many substrates. However, the role of the Src-homology 2 (SH2) domain in function of Fyn in neuronal migration remains poorly understood. Here, we demonstrate that the SH2 domain is essential for the action of Fyn in neuronal migration and cortical lamination. A point mutation in the Fyn SH2 domain (FynR176A) impaired neuronal migration and their final location in the cerebral cortex, by inducing neuronal aggregation and branching. Thus, we provide the first evidence of the Fyn SH2 domain contributing to neuronal migration and neuronal morphogenesis.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Lu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Xinde
authors.initials: X
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Lingzhen
authors.initials: L
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: An
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Duan
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Shanting
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000199:Actins / Q000378:metabolism; D019943:Amino Acid Substitution; D000818:Animals; D016466:CHO Cells; D002465:Cell Movement; D002540:Cerebral Cortex / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051076:Proto-Oncogene Proteins c-fyn / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D016596:Vinculin / Q000378:metabolism; D018909:src Homology Domains
chemical_list: D000199:Actins; D016596:Vinculin; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn