title: Effects of laser treatment on the expression of cytosolic proteins in the synovium of patients with osteoarthritis.
abstract: OBJECTIVE
  Low level laser therapy (LLLT) has been developed for non-invasive treatment of joint diseases. We have previously shown that LLLT influenced synovial protein expression in rheumatoid arthritis (RA). The aim of this study was to assess the effects of laser irradiation on osteoarthritic (OA) synovial protein expression.
  
  
  METHODS
  The synovial membrane samples removed from the knees of 6 OA patients were irradiated ex vivo using near infrared diode laser (807-811 nm; 25 J/cm(2) ). An untreated sample taken from the same patient served as control. Synovial protein separation and identification were performed by two-dimensional differential gel electrophoresis and mass spectrometry, respectively.
  
  
  RESULTS
  Eleven proteins showing altered expression due to laser irradiation were identified. There were three patients whose tissue samples demonstrated a significant increase (P < 0.05) in mitochondrial heat shock 60 kD protein 1 variant 1. The expression of the other proteins (calpain small subunit 1, tubulin alpha-1C and beta 2, vimentin variant 3, annexin A1, annexin A5, cofilin 1, transgelin, and collagen type VI alpha 2 chain precursor) significantly decreased (P < 0.05) compared to the control samples.
  
  
  CONCLUSIONS
  A single diode laser irradiation of the synovial samples of patients with osteoarthritis can statistically significantly alter the expression of some proteins in vitro. These findings provide some more evidence for biological efficacy of LLLT treatment, used for osteoarthritis.
authors.affiliation: Outpatient Clinic of Budavári Local Government, Unit of Rheumatology, Budapest, 1122, Hungary; National Institute of Rheumatology and Physiotherapy, Budapest, 1023, Hungary.
authors.forename: Klára
authors.initials: K
authors.lastname: Barabás
authors.forename: József
authors.initials: J
authors.lastname: Bakos
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Zeitler
authors.forename: Géza
authors.initials: G
authors.lastname: Bálint
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Nagy
authors.forename: Tamás
authors.initials: T
authors.lastname: Lakatos
authors.forename: Adrienna Katalin
authors.initials: AK
authors.lastname: Kékesi
authors.forename: Lajos
authors.initials: L
authors.lastname: Gáspár
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szekanecz
mesh_terms: D000368:Aged; D003600:Cytosol / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000628:therapy*; D011506:Proteins / Q000378:metabolism*; D013583:Synovial Membrane / Q000737:chemistry*
chemical_list: D011506:Proteins
keywords: ex vivo; laser therapy; proteomics; synovial membrane