title: A new alkaloid from the fruit of Nandina domestica Thunb.
abstract: A new steroidal alkaloid, (20S,22R,24R)-24-ethyl-3-oxocholest-4-en-22-amino, named as nandsterine (1), together with 10 known alkaloids, palmatine (2), O-methylbulbocapnine (3), nantenine (4), dehydronantenine (5), glaucine (6), didehydroglaucine (7), dehydrocorydaline (8), jatrorrhizine (9), magnoflorine (10) and berberine (11), was isolated from the fruit of Nandina domestica Thunb. Their structures were elucidated by using spectroscopic methods as well as by comparing with the published data. Compound 1 was a new class of steroidal alkaloid isolated from the family Berberidaceae, meanwhile compounds 2, 3, 6-8 and 10 were obtained from N. domestica for the first time. Compound 1 exhibited cytotoxicity against HL-60 cells (human leukaemia) with IC50 values of 52.1 μM.
authors.affiliation: a Key Laboratory of Modern Preparation of Traditional Chinese Medicines, Ministry of Education, Jiangxi University of Traditional Chinese Medicine , Nanchang 330004 , P.R. China.
authors.forename: Cai-Ying
authors.initials: CY
authors.lastname: Peng
authors.forename: Jian-Qun
authors.initials: JQ
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Shu
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001060:Aporphines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D028141:Berberidaceae / Q000737:chemistry*; D002782:Cholestenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D001060:Aporphines; D002782:Cholestenes; D004365:Drugs, Chinese Herbal; C000592992:nandsterine; C007232:dehydrocorydalin; C001670:magnoflorine; C005004:glaucine; C041615:nantenine
keywords: Nandina domestica Thunb; alkaloid; cytotoxicity