title: Sensitivity of real-time PCR performed on dried sera spotted on filter paper for diagnosis of leptospirosis.
abstract: In remote countries, leptospirosis confirmation is difficult because it requires the shipment of frozen samples to reference laboratories. The sensitivity of leptospirosis real-time PCR performed on filter paper-dried serum samples stored at ambient temperature was evaluated at 2 × 10(2) equivalent leptospires/ml (eqLeptospires/ml). This easy alternative procedure can be used to enhance the surveillance of leptospirosis.
authors.affiliation: Institut Louis Malardé, Tahiti, French Polynesia tnhan@ilm.pf.
authors.forename: T X
authors.initials: TX
authors.lastname: Nhan
authors.affiliation: Institut Louis Malardé, Tahiti, French Polynesia.
authors.forename: A
authors.initials: A
authors.lastname: Teissier
authors.affiliation: Institut Louis Malardé, Tahiti, French Polynesia.
authors.forename: C
authors.initials: C
authors.lastname: Roche
authors.affiliation: Institut Louis Malardé, Tahiti, French Polynesia.
authors.forename: D
authors.initials: D
authors.lastname: Musso
mesh_terms: D003890:Desiccation; D006801:Humans; D007922:Leptospirosis / Q000175:diagnosis*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010209:Paper; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature