title: Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification.
abstract: The selective extraction of endogenous serum peptides has been a challenge due to the high abundant proteins present in serum. Here a simple on-line extraction of peptides from human serum using strong cation-exchange diol silica restricted-access materials (SCX-RAM) coupled with two-dimensional RP-RP liquid chromatography mass spectrometry was developed. The operation of the on-line extraction system is simple to use and does not need complex equipments. The two-dimensional RP-RP was proved to be orthogonal and efficient to separate peptides extracted from human serum.
authors.affiliation: Key Laboratory Molecular Enzymology and Engineering, the Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China; Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: lianghaihu@jlu.edu.cn.
authors.forename: Lianghai
authors.initials: L
authors.lastname: Hu
authors.affiliation: Laboratory of BioSeparation, Institute of Clinical Chemistry, University Hospital Grosshadern, D-81366 Munich, Germany.
authors.forename: Karl-Siegfried
authors.initials: KS
authors.lastname: Boos
authors.affiliation: Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Ye
authors.affiliation: Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D006801:Humans; D007474:Ion Exchange; D008970:Molecular Weight; D009862:Online Systems; D010455:Peptides / Q000097:blood* / Q000737:chemistry; D044967:Serum / Q000737:chemistry; D012822:Silicon Dioxide; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides; D012822:Silicon Dioxide
keywords: Proteomics; Restrict-access materials; Sample extraction; Serum peptides; Two-dimensional