title: Quality control on the frontier.
abstract: In the world of high-throughput sequencing there are numerous challenges to effective data quality control. There are no single quality metrics which are appropriate in all conditions. Here we detail the different open source software used at the Exeter Sequencing Service to provide generic quality control information, as well as more specific metrics for genomic and transcriptomic libraries run on Illumina platforms.
authors.affiliation: Exeter Sequencing Service, Biosciences, College of Life and Environmental Science, University of Exeter Exeter, UK.
authors.forename: Konrad H
authors.initials: KH
authors.lastname: Paszkiewicz
authors.affiliation: Exeter Sequencing Service, Biosciences, College of Life and Environmental Science, University of Exeter Exeter, UK.
authors.forename: Audrey
authors.initials: A
authors.lastname: Farbos
authors.affiliation: Exeter Sequencing Service, Biosciences, College of Life and Environmental Science, University of Exeter Exeter, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: O'Neill
authors.affiliation: Exeter Sequencing Service, Biosciences, College of Life and Environmental Science, University of Exeter Exeter, UK.
authors.forename: Karen
authors.initials: K
authors.lastname: Moore
keywords: Illumina; best practice; core-facility; quality control; sequencing