title: Direct measurement of intracellular pressure.
abstract: A method to directly measure the intracellular pressure of adherent, migrating cells is described in this unit. This approach is based on the servo-null method where a microelectrode is introduced into the cell to directly measure the physical pressure of the cytoplasm. We also describe the initial calibration of the microelectrode, as well as the application of the method to cells migrating inside three-dimensional (3-D) extracellular matrix (ECM).
authors.affiliation: Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland.
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Petrie
authors.forename: Hyun
authors.initials: H
authors.lastname: Koo
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D005109:Extracellular Matrix; D006801:Humans; D008839:Microelectrodes; D011312:Pressure
keywords: extracellular matrix; fibroblasts; intracellular pressure; motility