title: Cellular membrane enrichment of self-assembling D-peptides for cell surface engineering.
abstract: We occasionally found that several self-assembling peptides containing D-amino acids would be preferentially enriched in cellular membranes at self-assembled stages while distributed evenly in the cytoplasma of cells at unassembled stages. Self-assembling peptides containing only Lamino acids distributed evenly in cytoplasma of cells at both self-assembled and unassembled stages. The self-assembling peptides containing D-amino acids could therefore be applied for engineering cell surface with peptides. More importantly, by integrating a protein binding peptide (a PDZ domain binding hexapeptide of WRESAI) with the self-assembling peptide containing D-amino acids, protein could also be introduced to the cell surface. This study not only provided a novel approach to engineer cell surface, but also highlighted the unusual properties and potential applications of self-assembling peptides containing D-amino acids in regenerative medicine, drug delivery, and tissue engineering.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), ‡College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Huaimin
authors.initials: H
authors.lastname: Wang
authors.forename: Youzhi
authors.initials: Y
authors.lastname: Wang
authors.forename: Aitian
authors.initials: A
authors.lastname: Han
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Cai
authors.forename: Nannan
authors.initials: N
authors.lastname: Xiao
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Ding
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D060846:Cell Engineering; D002462:Cell Membrane / Q000737:chemistry; D006801:Humans; D057139:Nanofibers; D054731:PDZ Domains; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D044968:Regenerative Medicine; D013499:Surface Properties
chemical_list: D000596:Amino Acids; D010455:Peptides