title: MicroRNA-222 promotes the proliferation and migration of cervical cancer cells.
abstract: OBJECTIVE
  This study aimed to investigate the role of small non-coding RNA-222 (microRNA-222; miR-222) in the development of cervical cancer (CC).
  
  
  METHODS
  Normal and CC specimens were obtained from 18 patients. HeLa and SiHa cells were grown in Dulbecco's modified Eagle's medium. RT-PCR, Western blot, migration assay, flow cytometry and immunofluorescence microscopy were used for analyses.
  
  
  RESULTS
  When compared with normal cervical tissues, miR-222 was upregulated in human CC, and the extent of up-regulation was associated with the extent and depth of CC invasion. Expression of miR-222 was inversely related to the expression of phosphatase and tensin homolog (PTEN) and p27. The reduced the expression of PTEN and p27 by miR-222 in HeLa cells and SiHa cells was associated with increased proliferation and migration of CC cells. The expression of proteins (E-cadherin and paxillin) related to the proliferation and migration was also elevated.
  
  
  CONCLUSIONS
  MiR-222 plays an important role in the tumorigenesis of CC, possibly by specifically down-regulating p27Kip1 and PTEN. Our findings suggest that miR-222 may serve as a new therapeutic target in CC.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Jiajing
authors.initials: J
authors.lastname: Cheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.forename: Fing
authors.initials: F
authors.lastname: Xing
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Wang
authors.forename: Qingying
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, No. 301 Middle Yanchang Rd., Zhabei Distinct, Shanghai, 200072, China jinqiujinqiu88@126.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Qiu
mesh_terms: D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008856:Microscopy, Fluorescence; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: D035683:MicroRNAs; C525710:MIR222, human