title: Interleukin-1 (IL-1) immunohistochemistry assay in oral squamous cell carcinoma.
abstract: This chapter describes an immunohistochemistry method to analyze interleukin-1 (IL-1) in oral squamous cell carcinoma. The described protocol has been optimized for IL-1 detection in formalin-fixed paraffin-embedded oral tissue sections by light microscopy. A few common pitfalls and problems associated with immunohistochemical staining are discussed.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Showa University School of Dentistry, Kitasenzoku 2-1-1, Ota City, Tokyo, 145-8515, Japan, kamatanitakaaki@gmail.com.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kamatani
mesh_terms: D002021:Buffers; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology; D005557:Formaldehyde; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry / Q000592:standards*; D007375:Interleukin-1 / Q000032:analysis* / Q000276:immunology; D008867:Microtomy; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology; D016612:Paraffin Embedding; D016707:Tissue Fixation
chemical_list: D002021:Buffers; D007375:Interleukin-1; D005557:Formaldehyde