title: Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.
abstract: The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Elaine M S
authors.initials: EM
authors.lastname: Dorneles
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Jordana A
authors.initials: JA
authors.lastname: Santana
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Dayana
authors.initials: D
authors.lastname: Ribeiro
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Fernanda Alves
authors.initials: FA
authors.lastname: Dorella
authors.affiliation: Embrapa Gado de Leite, Empresa Brasileira de Pesquisa Agropecuária, Juiz de Fora, Brazil; Departmento de Medicina Veterinária, Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil.
authors.forename: Alessandro S
authors.initials: AS
authors.lastname: Guimarães
authors.affiliation: Department of Toxicology and Forensic Medicine, Cairo University, Cairo, Egypt.
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Moawad
authors.affiliation: Department of Toxicology and Forensic Medicine, Cairo University, Cairo, Egypt.
authors.forename: Salah A
authors.initials: SA
authors.lastname: Selim
authors.affiliation: Centro Biomédico, Faculdade de Ciências Médicas, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Ana Luiza M
authors.initials: AL
authors.lastname: Garaldi
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.affiliation: Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia Universidade, Estadual Paulista, Botucatu, Brazil.
authors.forename: Márcio G
authors.initials: MG
authors.lastname: Ribeiro
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Aurora M G
authors.initials: AM
authors.lastname: Gouveia
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Marcos B
authors.initials: MB
authors.lastname: Heinemann
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Andrey P
authors.initials: AP
authors.lastname: Lage
mesh_terms: D000820:Animal Diseases / Q000382:microbiology; D000818:Animals; D016000:Cluster Analysis; D003354:Corynebacterium Infections / Q000662:veterinary; D016925:Corynebacterium pseudotuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D021901:DNA, Intergenic; D005838:Genotype; D058889:Molecular Typing; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D021901:DNA, Intergenic