title: RNA extraction from equine samples for equine influenza virus.
abstract: The primary goals of this chapter are to discuss common viral RNA isolation and purification methods that are routinely used by various diagnostic laboratories, to highlight the advantages and drawbacks of each method, and to identify the most suitable and reliable method to increase the sensitivity and specificity of RT-PCR assays for the detection of equine influenza virus (EIV) in clinical specimens. Our experiences and review of literature show that magnetic bead-based nucleic extraction methods (manual and automatic) work well for isolation and purification of EIV RNA from nasal swab specimens. Furthermore, most of the information presented in this chapter could be directly applicable to isolation and purification of nucleic acids (both DNA and RNA) from other equine clinical samples.
authors.affiliation: Department of Veterinary Science, University of Kentucky, 108 Maxwell H. Gluck Equine Research Center, Lexington, KY, 40546-0099, USA, ubalasuriya@uky.edu.
authors.forename: Udeni B R
authors.initials: UB
authors.lastname: Balasuriya
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D002725:Chloroform / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006146:Guanidines / Q000737:chemistry; D006736:Horses / Q000821:virology*; D009980:Influenza A virus; D059346:Magnets / Q000737:chemistry; D009666:Nose / Q000821:virology; D019800:Phenol / Q000737:chemistry; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry; D013861:Thiocyanates / Q000737:chemistry
chemical_list: D006146:Guanidines; D012367:RNA, Viral; D013861:Thiocyanates; D019800:Phenol; C054436:guanidine thiocyanate; D012822:Silicon Dioxide; D002725:Chloroform