title: Adult offspring of high-fat diet-fed dams can have normal glucose tolerance and body composition.
abstract: Maternal high-fat diet consumption and obesity have been shown to program long-term obesity and lead to impaired glucose tolerance in offspring. Many rodent studies, however, use non-purified, cereal-based diets as the control for purified high-fat diets. In this study, primiparous ICR mice were fed purified control diet (10-11 kcal% from fat of lard or butter origin) and lard (45 or 60 kcal% fat) or butter (32 or 60 kcal% fat)-based high-fat diets for 4 weeks before mating, throughout pregnancy, and for 2 weeks of nursing. Before mating, female mice fed the 32 and 60% butter-based high-fat diets exhibited impaired glucose tolerance but those females fed the lard-based diets showed normal glucose disposal following a glucose challenge. High-fat diet consumption by female mice of all groups decreased lean to fat mass ratios during the 4th week of diet treatment compared with those mice consuming the 10-11% fat diets. All females were bred to male mice and pregnancy and offspring outcomes were monitored. The body weight of pups born to 45% lard-fed dams was significantly increased before weaning, but only female offspring born to 32% butter-fed dams exhibited long-term body weight increases. Offspring glucose tolerance and body composition were measured for at least 1 year. Minimal, if any, differences were observed in the offspring parameters. These results suggest that many variables should be considered when designing future high-fat diet feeding and maternal obesity studies in mice.
authors.affiliation: 1 Graduate Center for Nutritional Sciences, College of Medicine, University of Kentucky, Lexington, KY, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Platt
authors.affiliation: 2 Department of Biostatistics, College of Public Health, University of Kentucky, Lexington, KY, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Charnigo
authors.affiliation: 1 Graduate Center for Nutritional Sciences, College of Medicine, University of Kentucky, Lexington, KY, USA.
authors.forename: K J
authors.initials: KJ
authors.lastname: Pearson
mesh_terms: D000367:Age Factors; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001823:Body Composition / Q000502:physiology*; D059305:Diet, High-Fat / Q000009:adverse effects / Q000639:trends*; D005260:Female; D018149:Glucose Intolerance / Q000097:blood / Q000139:chemically induced; D005951:Glucose Tolerance Test / Q000379:methods; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D011897:Random Allocation
chemical_list: D001786:Blood Glucose