title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of a ferredoxin/flavodoxin-NADP(H) oxidoreductase (Bc0385) from Bacillus cereus.
abstract: Ferredoxin/flavodoxin-NADP(H) oxidoreductases (FNRs) are key enzymes involved in catalysing electron transfer between ferredoxins/flavodoxins and NAD(P)H/NAD(P)+. In Bacillus cereus there are three genes that may encode FNRs, and the Bc0385 FNR has been cloned, overexpressed, purified and successfully crystallized in its NADPH/NADP+-free form. Diffraction data have been collected to 2.5 Å resolution from crystals belonging to the orthorhombic space group P2₁2₁2, with unit-cell parameters a=57.2, b=164.3, c=95.0 Å, containing two FNR molecules in the asymmetric unit. The structure of the Bc0385 FNR has been solved by molecular replacement, and is a member of the homodimeric thioredoxin reductase-like class of FNRs.
authors.affiliation: Department of Biosciences, Section for Biochemistry and Molecular Biology, University of Oslo, PO Box 1066, Blindern, NO-0316 Oslo, Norway.
authors.forename: Silje
authors.initials: S
authors.lastname: Skråmo
authors.affiliation: Department of Biosciences, Section for Biochemistry and Molecular Biology, University of Oslo, PO Box 1066, Blindern, NO-0316 Oslo, Norway.
authors.forename: Hans Petter
authors.initials: HP
authors.lastname: Hersleth
authors.affiliation: Department of Biosciences, Section for Biochemistry and Molecular Biology, University of Oslo, PO Box 1066, Blindern, NO-0316 Oslo, Norway.
authors.forename: Marta
authors.initials: M
authors.lastname: Hammerstad
authors.affiliation: Department of Biosciences, Section for Biochemistry and Molecular Biology, University of Oslo, PO Box 1066, Blindern, NO-0316 Oslo, Norway.
authors.forename: K Kristoffer
authors.initials: KK
authors.lastname: Andersson
authors.affiliation: Department of Biosciences, Section for Biochemistry and Molecular Biology, University of Oslo, PO Box 1066, Blindern, NO-0316 Oslo, Norway.
authors.forename: Åsmund K
authors.initials: ÅK
authors.lastname: Røhr
mesh_terms: D000595:Amino Acid Sequence; D001409:Bacillus cereus / Q000201:enzymology*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry*; D008969:Molecular Sequence Data
chemical_list: D005287:Ferredoxin-NADP Reductase
keywords: Bacillus cereus; electron transfer; ferredoxin/flavodoxin-NADP(H) oxidoreductase; flavoprotein