title: Fluorescent hydrogen peroxide sensor based on cupric oxide nanoparticles and its application for glucose and L-lactate detection.
abstract: A novel fluorescent hydrogen peroxide sensor was developed based on the peroxidase-like activity of cupric oxide nanoparticles. Cupric oxide nanoparticles effectively catalyzed the decomposition of hydrogen peroxide into hydroxyl radicals. Then terephthalic acid was oxidized by hydroxyl radical to form a highly fluorescent product. The linear range of hydrogen peroxide estimated to be 5.0 × 10(-6)-2.0 × 10(-4)M with a detection limit of 3.4 × 10(-7)M. Moreover, this detection system enabled the sensing of analytes which can enzymatically generate hydrogen peroxide. By coupling the oxidation of glucose or L-lactate catalyzed by their corresponding oxidase enzymes with terephthalic acid oxidation catalyzed by cupric oxide nanoparticles, sensitive assays of glucose and l-lactate with detection limits of 1.0 × 10(-6) and 4.5 × 10(-8)M were realized. The successful applications of this approach in human serum samples have also been demonstrated.
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Hu
authors.affiliation: Department of Laboratory Medicine, Second Hospital of Fuzhou, Fuzhou 350007, China.
authors.forename: Yin-Huan
authors.initials: YH
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Hao-Hua
authors.initials: HH
authors.lastname: Deng
authors.affiliation: Department of Laboratory Medicine, Second Hospital of Fuzhou, Fuzhou 350007, China.
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Hong
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Ai-Lin
authors.initials: AL
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Xia
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China. Electronic address: chenandhu@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D003300:Copper / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D019344:Lactic Acid / Q000097:blood*; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010795:Phthalic Acids / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D005456:Fluorescent Dyes; D010795:Phthalic Acids; D019344:Lactic Acid; C011363:terephthalic acid; D003300:Copper; D006861:Hydrogen Peroxide; C030973:cupric oxide
keywords: Cupric oxide nanoparticles; Fluorimetry; Glucose; Hydrogen peroxide; Terephthalic acid; l-lactate