title: Mammalian cell-transforming potential of traffic-linked ultrafine particulate matter PM0.056 in urban roadside atmosphere.
abstract: We examined the clastogenic and cell-transforming potential of ultrafine particulate matter fraction PM0.056 of urban ambient aerosol using mammalian cells. PM1.0, PM0.56 and PM0.056 fractions were sampled from roadside atmosphere of an urban area using the cascade impactor MOUDI-NR-110. The potential to induce cytotoxicity, DNA damage and micronuclei formation was examined at the test concentrations of 3, 6, 12.5, 25, 50 and 100 μg/ml using the 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, the plasmid relaxation assay and the C3H10T1/2 (10T1/2) cells. The cell-transforming potential was investigated in vitro using 10T1/2 cell transformation assay and the soft agar assay. PM1, PM0.56 and PM0.056 fractions were found to be toxic in dose-dependent manner. These induced cytotoxicity at five test concentrations, the ultrafine particle fraction PM0.056 showed greater cytotoxic potential. PM0.056 induced micronucleus formation in 10T1/2 cells. The effect was statistically significant. The DNA-damaging potential was measured in a plasmid relaxation assay. Both fine and ultrafine particle fraction PM0.56 and PM0.056 displayed greater effect as compared to larger PM1 fraction. DNA damage was found to be dependent on particulate matter intrinsic pro-oxidant chemicals. The ability of the ultrafine particle fraction PM0.056 to induce morphological cell transformation was demonstrated by significant and dose-dependent increases in type III focus formation by morphologically transformed cells in culture flasks and their clonal expansion in soft agar. It is concluded that the traffic-linked ultrafine particle fraction PM0.056 in the atmosphere by the roadside of an urban area is clastogenic and able to induce morphological transformation of mammalian cells.
authors.affiliation: Environmental Carcinogenesis Laboratory, CSIR-Indian Institute of Toxicology Research, MG Marg, P Box 80, Lucknow 226001, India and Medical Elementology and Toxicology Division, Hamdard University, New Delhi 110062, India.
authors.forename: Mukesh K
authors.initials: MK
authors.lastname: Verma
authors.affiliation: Environmental Carcinogenesis Laboratory, CSIR-Indian Institute of Toxicology Research, MG Marg, P Box 80, Lucknow 226001, India and.
authors.forename: Shiv
authors.initials: S
authors.lastname: Poojan
authors.affiliation: Medical Elementology and Toxicology Division, Hamdard University, New Delhi 110062, India.
authors.forename: Sarwat
authors.initials: S
authors.lastname: Sultana
authors.affiliation: Environmental Carcinogenesis Laboratory, CSIR-Indian Institute of Toxicology Research, MG Marg, P Box 80, Lucknow 226001, India and sushilkumar@iitr.res.in sushilkumar.iitr@gmail.com.
authors.forename: Sushil
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000393:Air Pollutants / Q000737:chemistry / Q000633:toxicity; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods*; D051379:Mice; D015162:Micronucleus Tests; D010316:Particle Size; D052638:Particulate Matter / Q000737:chemistry* / Q000633:toxicity
chemical_list: D000393:Air Pollutants; D052638:Particulate Matter