title: Isolation of mesenchymal stromal cells from extraembryonic tissues and their characteristics.
abstract: We describe a method of isolation of human mesenchymal stromal cells from the umbilical cord (Wharton's jelly) and human placenta: amnion, placental villi, and trophoblast. Morphology, immunophenotypic characteristics, and differentiation potencies of isolated cells were studied. The capacity of mesenchymal stromal cells from extraembryonic tissues to osteogenic, adipogenic, and chondrogenic differentiation was demonstrated and the dynamics of this process was described. The isolated cells met the criteria for multipotent mesenchymal stem cells.
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Veryasov
authors.forename: A M
authors.initials: AM
authors.lastname: Savilova
authors.forename: O A
authors.initials: OA
authors.lastname: Buyanovskaya
authors.forename: M M
authors.initials: MM
authors.lastname: Chulkina
authors.forename: S V
authors.initials: SV
authors.lastname: Pavlovich
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000276:immunology; D000650:Amnion / Q000166:cytology* / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000276:immunology; D002824:Chorionic Villi / Q000276:immunology; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000276:immunology; D010011:Osteocytes / Q000166:cytology / Q000276:immunology; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology; D014470:Umbilical Cord / Q000166:cytology* / Q000276:immunology; D059631:Wharton Jelly / Q000166:cytology* / Q000276:immunology
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D006684:HLA-DR Antigens