title: Injectable hyaluronic acid hydrogel for 19F magnetic resonance imaging.
abstract: We report on a 19F labeled injectable hyaluronic acid (HA) hydrogel that can be monitored by both 1H and 19F MR imaging. The HA based hydrogel formed via carbazone reaction can be obtained within a minute by simple mixing of HA-carbazate and HA-aldehyde derivatized polymers. 19F contrast agent was linked to with carbazate and thiol dually functionalized HA via orthogonal Michael addition reaction which afforded cross-linkable and 19F labeled HA. The 19F labeling of HA polymer did not affect the mechanical properties of the formed hydrogel. As a result, the shape of a hydrogel sample could be imaged very well by both 1H MRI and high resolution 19F MRI. This hydrogel has high potential in clinical applications since it is injectable, biocompatible, and can be tracked in a minimally invasive manner. The present approach can be applied in preparation of injectable 19F labeled hydrogel biomaterials from other natural biomacromolecules.
authors.affiliation: Department of Chemistry-Angstrom, Uppsala University, Uppsala, Sweden.
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Radiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands; Department of Urology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Chemistry-Angstrom, Uppsala University, Uppsala, Sweden.
authors.forename: Sujit
authors.initials: S
authors.lastname: Kootala
authors.affiliation: Department of Chemistry-Angstrom, Uppsala University, Uppsala, Sweden.
authors.forename: Jöns
authors.initials: J
authors.lastname: Hilborn
authors.affiliation: Department of Radiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
authors.forename: Arend
authors.initials: A
authors.lastname: Heerschap
authors.affiliation: Department of Chemistry-Angstrom, Uppsala University, Uppsala, Sweden. Electronic address: dmitri.ossipov@kemi.uu.se.
authors.forename: Dmitri
authors.initials: D
authors.lastname: Ossipov
mesh_terms: D066235:Fluorine-19 Magnetic Resonance Imaging / Q000379:methods*; D006820:Hyaluronic Acid / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D020100:Hydrogels / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D007267:Injections
chemical_list: D020100:Hydrogels; D006820:Hyaluronic Acid
keywords: (19)F MRI; Chemoselective reactions; Hyaluronan; Hydrogel; Injectable