title: Assessing effect of three herbal medicines in second and third degree burns in rats and comparison with silver sulfadiazine ointment.
abstract: BACKGROUND
  Assessing effect of three herbal medicines on healing of deep second and third degree burns in rats and their comparison with silver sulfadiazine group.
  
  
  METHODS
  40 rats were randomly assigned to one of the four groups, each group 10 rats. A deep second degree burn and a third degree burn were induced on the lower back and upper back of each rat under standard burning procedure, respectively. The burns were daily dressed with Robacin in group 1, silver sulfadiazine in group 2, aloe vera extract in group 3, and Rimojen in group 4. Responses to the treatment were assessed by digital photography during the treatment until day 32. Histological parameters (PMN, epithelialization, fibrosis, and angiogenesis) were assessed after the scar biopsy at the end of the research.
  
  
  RESULTS
  On the basis of the taken photos, the wound had better healing in Robacin group. Also, speed of healing was better in aloe vera group than silver sulfadiazine and Rimojen groups. In terms of wound surface area maximal improvement was observed at the same time in the second and third degree burn wounds in Robacin group, in the second degree wound of aloe vera and Rimojen groups, and in the third degree wound of aloe vera and silver sulfadiazine groups. In pathological respects, epithelialization was more evident in both wounds of aloe vera group and third degree burn of Robacin group. In both wounds of Robacin group (second and third degree), the extend of angiogenesis and fibrosis was significantly less than other groups; but, inflammation was at a less level in third degree of silver, second degree of Rimogen and aloe vera, and third degree of aloe vera groups.
  
  
  CONCLUSIONS
  In histological survey, minimal rate of angiogenesis and fibrosis was seen in Robacin group, which indicated less wound scar in this group. Healing speed of the burn wound was also higher in Robacin group.
authors.affiliation: Faculty of Plastic and Reconstructive Surgery, Burn Research Centre, Iran University of Medical Sciences, Tehran, Iran. Electronic address: akhoondinasab@yahoo.com.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Akhoondinasab
authors.affiliation: Burn Research Centre, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Khodarahmi
authors.affiliation: Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Motahhare
authors.initials: M
authors.lastname: Akhoondinasab
authors.affiliation: Medicine, Quran and Hadith Research Center and Department of Community Medicine, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Saberi
authors.affiliation: Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Iranpour
mesh_terms: D000504:Aloe; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002056:Burns / Q000188:drug therapy / Q000473:pathology*; D020838:Calendula; D002921:Cicatrix / Q000473:pathology; D005355:Fibrosis; D009389:Neovascularization, Pathologic / Q000473:pathology*; D009824:Ointments; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D027826:Rosa; D012837:Silver Sulfadiazine / Q000494:pharmacology*; D012867:Skin / Q000473:pathology*; D046930:Thymus Plant; D014885:Waxes / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000891:Anti-Infective Agents, Local; D009824:Ointments; D010936:Plant Extracts; D014885:Waxes; C038228:beeswax; D012837:Silver Sulfadiazine
keywords: Animal model; Burn ointment; Herbal medicines; Silver sulfadiazine