title: Atg1 kinase organizes autophagosome formation by phosphorylating Atg9.
abstract: The conserved Ser/Thr kinase Atg1/ULK1 plays a crucial role in the regulation of autophagy. However, only very few Atg1 targets have been identified, impeding elucidation of the mechanisms by which Atg1 regulates autophagy. In our study, we determined the Saccharomyces cerevisiae Atg1 consensus phosphorylation sequence using a peptide array-based approach. Among proteins containing this sequence we identified Atg9, another essential component of the autophagic machinery. We showed that phosphorylation of Atg9 by Atg1 is required for phagophore elongation, shedding light on the mechanism by which Atg1 regulates early steps of autophagy.
authors.affiliation: Max F Perutz Laboratories; University of Vienna; Vienna, Austria.
authors.forename: Daniel
authors.initials: D
authors.lastname: Papinski
authors.affiliation: Max F Perutz Laboratories; University of Vienna; Vienna, Austria.
authors.forename: Claudine
authors.initials: C
authors.lastname: Kraft
mesh_terms: D001343:Autophagy; D000071183:Autophagy-Related Proteins; D008565:Membrane Proteins / Q000378:metabolism*; D008954:Models, Biological; D010588:Phagosomes / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: C434065:ATG9 protein, S cerevisiae; D000071183:Autophagy-Related Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C477823:ATG1 protein, S cerevisiae
keywords: Atg1/ULK1 kinase; Atg18/WIPI2; Atg8; Atg9; Cvt pathway; autophagy