title: Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens.
abstract: Inclusion of prebiotics in the diet is known to be advantageous, with positive influences both on health and growth. The current study investigated the differences in the hepatic transcriptome profiles between chickens supplemented with inulin (a storage carbohydrate found in many plants) and controls. Liver is a major metabolic organ and has been previously reported to be involved in the modification of the lipid metabolism in chickens fed with inulin. A nutrigenomic approach through the analysis of liver RNA hybridized to the Affymetrix GeneChip Chicken Genome Array identified 148 differentially expressed genes among both groups: 104 up-regulated (≥ 1.4-fold) and 44 down-regulated (≤ 0.6-fold). Quantitative real-time PCR analysis validated the microarray expression results for five out of seven genes tested. The functional annotation analyses revealed a number of genes, processes and pathways with putative involvement in chicken growth and performance, while reinforcing the immune status of animals, and fostering the production of long chain fatty acids in broilers supplemented with 5 g of inulin kg(-1) diet. As far as we are aware, this is the first report of a microarray based gene expression study on the effect of dietary inulin supplementation, supporting further research on the use of this prebiotic on chicken diets as a useful alternative to antibiotics for improving performance and general immunity in poultry farming, along with a healthier meat lipid profile.
authors.affiliation: Nutrigenómica Animal, Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Sevane
authors.affiliation: Nutrigenómica Animal, Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Federica
authors.initials: F
authors.lastname: Bialade
authors.affiliation: Nutrigenómica Animal, Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Velasco
authors.affiliation: Nutrigenómica Animal, Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Almudena
authors.initials: A
authors.lastname: Rebolé
authors.affiliation: Nutrigenómica Animal, Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Rodríguez
authors.affiliation: Nutrigenómica Animal, Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Luís T
authors.initials: LT
authors.lastname: Ortiz
authors.affiliation: Nutrigenómica Animal, Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Cañón
authors.affiliation: Nutrigenómica Animal, Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Dunner
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002645:Chickens / Q000235:genetics*; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D019587:Dietary Supplements; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D007444:Inulin / Q000378:metabolism* / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D056692:Prebiotics; D015203:Reproducibility of Results; D059467:Transcriptome
chemical_list: D056692:Prebiotics; D007444:Inulin