title: Identification and Validation of Expressed Sequence Tags from Pigeonpea (Cajanus cajan L.) Root.
abstract: Pigeonpea (Cajanus cajan (L) Millsp.) is an important food legume crop of rain fed agriculture in the arid and semiarid tropics of the world. It has deep and extensive root system which serves a number of important physiological and metabolic functions in plant development and growth. In order to identify genes associated with pigeonpea root, ESTs were generated from the root tissues of pigeonpea (GRG-295 genotype) by normalized cDNA library. A total of 105 high quality ESTs were generated by sequencing of 250 random clones which resulted in 72 unigenes comprising 25 contigs and 47 singlets. The ESTs were assigned to 9 functional categories on the basis of their putative function. In order to validate the possible expression of transcripts, four genes, namely, S-adenosylmethionine synthetase, phosphoglycerate kinase, serine carboxypeptidase, and methionine aminopeptidase, were further analyzed by reverse transcriptase PCR. The possible role of the identified transcripts and their functions associated with root will also be a valuable resource for the functional genomics study in legume crop.
authors.affiliation: Vidya Pratishthan's School of Biotechnology, Vidyanagari, Baramati, Pune 413133, India ; Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.
authors.forename: Ravi Ranjan
authors.initials: RR
authors.lastname: Kumar
authors.affiliation: Institute of Biotechnology, Acharya N. G. Ranga Agricultural University, Hyderabad 500030, India.
authors.forename: Shailesh
authors.initials: S
authors.lastname: Yadav
authors.affiliation: Institute of Biotechnology, Acharya N. G. Ranga Agricultural University, Hyderabad 500030, India.
authors.forename: Shourabh
authors.initials: S
authors.lastname: Joshi
authors.affiliation: Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.
authors.forename: Prithviraj P
authors.initials: PP
authors.lastname: Bhandare
authors.affiliation: Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Patil
authors.affiliation: Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.
authors.forename: Pramod B
authors.initials: PB
authors.lastname: Kulkarni
authors.affiliation: Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Sonkawade
authors.affiliation: Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.
authors.forename: G R
authors.initials: GR
authors.lastname: Naik