title: A chicken influenza virus recognizes fucosylated α2,3 sialoglycan receptors on the epithelial cells lining upper respiratory tracts of chickens.
abstract: Influenza viruses recognize sialoglycans as receptors. Although viruses isolated form chickens preferentially bind to sialic acid α2,3 galactose (SAα2,3Gal) glycans as do those of ducks, chickens were not experimentally infected with viruses isolated from ducks. A chicken influenza virus, A/chicken/Ibaraki/1/2005 (H5N2) (Ck/IBR) bound to fucose-branched SAα2,3Gal glycans, whereas the binding towards linear SAα2,3Gal glycans was weak. On the epithelial cells of the upper respiratory tracts of chickens, fucose-branched SAα2,3Gal glycans were detected, but not linear SAα2,3Gal glycans. The growth of Ck/IBR in MDCK-FUT cells, which were genetically prepared to express fucose-branched SAα2,3Gal glycans, was significantly higher than that in the parental MDCK cells. The present results indicate that fucose-branched SAα2,3Gal glycans existing on the epithelial cells lining the upper respiratory tracts of chickens are critical for recognition by Ck/IBR.
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan; Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hiono
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan; Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Okamatsu
authors.affiliation: Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan; Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Nishihara
authors.affiliation: Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan; Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Takase-Yoden
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan; Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan; Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan; Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan. Electronic address: kida@vetmed.hokudai.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
mesh_terms: D000818:Animals; D002645:Chickens; D004847:Epithelial Cells / Q000737:chemistry* / Q000821:virology*; D005690:Galactose / Q000031:analogs & derivatives* / Q000378:metabolism; D009975:Orthomyxoviridae / Q000502:physiology*; D011991:Receptors, Virus / Q000378:metabolism*; D012137:Respiratory System / Q000821:virology; D053585:Virus Attachment
chemical_list: D011991:Receptors, Virus; C542734:sialylalpha(2-3)galactose; D005690:Galactose
keywords: Avian influenza virus; Fucosylation; Hemagglutinin; Interspecies transmission; Receptor