title: miR-27 inhibits adipocyte differentiation via suppressing CREB expression.
abstract: miR-27 plays a negative role in the regulation of adipogenesis. However, the molecular mechanism still remains to be clarified. In the present study, we found that miR-27 inhibits adipogenesis partially by repressing the early adipogenic transcription factor cAMP response element-binding protein by directly targeting its 3' untranslated region. In addition, we demonstrated that tumor necrosis factor-α (TNF-α) treatment up-regulates miR-27 through the NF-κB pathway. Furthermore, anti-miR-27 reduces the TNF-α-induced inhibition of adipogenesis. Simultaneously, the levels of miR-27 expression were decreased in mature adipocytes of obese mice when compared with lean mice. Our data revealed a novel mechanism of miR-27 in the regulation of adipogenesis.
authors.affiliation: Luoyang Orthopedic-Traumatological Hospital, Luoyang 471000, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Luoyang Orthopedic-Traumatological Hospital, Luoyang 471000, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Luoyang Orthopedic-Traumatological Hospital, Luoyang 471000, China.
authors.forename: Xingpo
authors.initials: X
authors.lastname: Ding
authors.affiliation: Luoyang Orthopedic-Traumatological Hospital, Luoyang 471000, China.
authors.forename: Huichao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Luoyang Orthopedic-Traumatological Hospital, Luoyang 471000, China.
authors.forename: Xiantao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Luoyang Orthopedic-Traumatological Hospital, Luoyang 471000, China.
authors.forename: Hanzheng
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Luoyang Orthopedic-Traumatological Hospital, Luoyang 471000, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai 200031, China shliu@sibs.ac.cn liuyouwendr@sina.com.
authors.forename: Sanhong
authors.initials: S
authors.lastname: Liu
authors.affiliation: Luoyang Orthopedic-Traumatological Hospital, Luoyang 471000, China shliu@sibs.ac.cn liuyouwendr@sina.com.
authors.forename: Youwen
authors.initials: Y
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions; D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism; D009765:Obesity / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
chemical_list: D020413:3' Untranslated Regions; C492004:Creb1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D035683:MicroRNAs; C540674:Mirn27 microRNA, mouse; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha
keywords: 3T3-L1 cells; TNF-α; adipogenesis; miR-27