title: Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine.
abstract: This communication describes a general approach for site-specific fluorescence labelling of RNA using a cytidine triphosphate (CTP) analogue derivatized with a trans-cyclooctene group. The analogue was efficiently incorporated into a model RNA strand using in vitro transcription. Bio-orthogonal reaction with fluorescein-labelled tetrazine was utilized to fluorescently tag the synthetic RNA strand.
authors.affiliation: Department of Chemistry, University at Albany, SUNY, 1400 Washington Avenue, Albany, NY 12222, USA. mroyzen@albany.edu.
authors.forename: P N
authors.initials: PN
authors.lastname: Asare-Okai
authors.forename: E
authors.initials: E
authors.lastname: Agustin
authors.forename: D
authors.initials: D
authors.lastname: Fabris
authors.forename: M
authors.initials: M
authors.lastname: Royzen
mesh_terms: D034242:Cyclooctanes / Q000737:chemistry*; D003570:Cytidine Triphosphate / Q000031:analogs & derivatives; D005453:Fluorescence; D012313:RNA / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013777:Tetrazoles / Q000737:chemistry*
chemical_list: D034242:Cyclooctanes; D013777:Tetrazoles; D012313:RNA; D003570:Cytidine Triphosphate