title: Osteoinduction of biphasic calcium phosphate scaffolds in a nude mouse model.
abstract: Bioceramics combined with isolated stem cells, or with total bone marrow, constitute the main strategies under consideration in the field of bone tissue engineering. In the present preclinical study, two biphasic calcium phosphate scaffolds currently on the market, MBCP® and MBCP+®, with different hydroxyapatite/β-tricalcium phosphate ratio, were implanted ectopically in a nude mouse model. These scaffolds were supplemented either with human mesenchymal stromal cells, or with human total bone marrow, or rat total bone marrow. Biomaterials alone were found to have potentially low, but non-zero, osteoinductive properties, while biomaterials associated with total bone marrow consistently improved osteoinduction in comparison with high concentrations of isolated human stromal cells.
authors.affiliation: INSERM UMRS 791, University of Nantes, Nantes, France Biomatlante SA, Vigneux-de-Bretagne, France thomas.miramond@univ-nantes.fr.
authors.forename: T
authors.initials: T
authors.lastname: Miramond
authors.affiliation: INSERM UMRS 791, University of Nantes, Nantes, France Departments of Stomatology and Maxillofacial Surgery, Nantes University Hospital, Nantes, France.
authors.forename: P
authors.initials: P
authors.lastname: Corre
authors.affiliation: Biomatlante SA, Vigneux-de-Bretagne, France.
authors.forename: P
authors.initials: P
authors.lastname: Borget
authors.affiliation: Biomatlante SA, Vigneux-de-Bretagne, France.
authors.forename: F
authors.initials: F
authors.lastname: Moreau
authors.affiliation: INSERM UMRS 791, University of Nantes, Nantes, France.
authors.forename: J
authors.initials: J
authors.lastname: Guicheux
authors.affiliation: INSERM UMRS 791, University of Nantes, Nantes, France.
authors.forename: G
authors.initials: G
authors.lastname: Daculsi
authors.affiliation: INSERM UMRS 791, University of Nantes, Nantes, France.
authors.forename: P
authors.initials: P
authors.lastname: Weiss
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D016026:Bone Marrow Transplantation; D001861:Bone Regeneration; D005260:Female; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D016348:Osseointegration; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate
keywords: Osteoinduction; biomaterials; bone tissue engineering; calcium phosphate; stem cells