title: Identification of a New Delhi metallo-β-lactamase-4 (NDM-4)-producing Escherichia coli in Italy.
abstract: BACKGROUND
  During June-July 2012, six imipenem-resistant Escherichia coli isolates were isolated from two patients hospitalized in a ward of one large tertiary-care hospital in Genoa, Italy. Genetic features associated with blaNDM-4 gene were investigated.
  
  
  RESULTS
  The isolates exhibited the same PFGE profile and a multidrug-resistant (MDR) phenotype to aminoglycosides, fluoroquinolones, and β-lactams. The strains produced the NDM-4 carbapenemase and the blaNDM-4 gene was part of the variable region of a class 1 integron. MLST analysis revealed that all isolates belonged to sequence type 405 (ST405).
  
  
  CONCLUSIONS
  This is the first report on the emergence of an MDR strain of E.coli producing the NDM-4 MBL in Italy.
authors.forename: Erika
authors.initials: E
authors.lastname: Coppo
authors.forename: Valerio
authors.initials: V
authors.lastname: Del Bono
authors.forename: Francesco
authors.initials: F
authors.lastname: Ventura
authors.forename: Marco
authors.initials: M
authors.lastname: Camera
authors.forename: Giovanni
authors.initials: G
authors.lastname: Orengo
authors.forename: Claudio
authors.initials: C
authors.lastname: Viscoli
authors.affiliation: Microbiology Unit DISC, University of Genoa, IRCCS AOU San Martino-IST, Largo R, Benzi 10, 16132 Genoa, Italy. anna.marchese@unige.it.
authors.forename: Anna
authors.initials: A
authors.lastname: Marchese
mesh_terms: D000368:Aged; D001426:Bacterial Proteins / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007558:Italy; D008297:Male; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D001618:beta-Lactamases / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D015378:Imipenem; D001618:beta-Lactamases; C063912:carbapenemase