title: Sodium benzoate and potassium sorbate preservatives in Iranian doogh.
abstract: Sodium benzoate and potassium sorbate are two common preservatives used in Iran, yet use of these preservatives in doogh (Iranian dairy-based drink) is forbidden according to national standards. The aim of this study was to consider the presence of these preservatives in doogh by high-performance liquid chromatography with UV detection (HPLC-UV). The method was performed using a C18 column and detection at 225 nm. The mobile phase contained ammonium acetate buffer (pH = 4.2) and acetonitrile (80:20 v/v). The survey included 130 samples of doogh for identification and quantification of the named preservatives. All samples contained sodium benzoate, but potassium sorbate was detected in only 13% of them. The means of benzoate and sorbate were 21.3 ± 2.7 and 13.3 ± 39.6 mg kg(-1), respectively. The limits of detection were 2 and 40 ng g(-1) for benzoate and sorbate, respectively. Results indicate that sodium benzoate may occur in doogh naturally.
authors.affiliation: a Food and Drug Administration , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: F
authors.initials: F
authors.lastname: Zamani Mazdeh
authors.forename: F
authors.initials: F
authors.lastname: Esmaeili Aftabdari
authors.forename: Z
authors.initials: Z
authors.lastname: Moradi-Khatoonabadi
authors.forename: M
authors.initials: M
authors.lastname: Shaneshin
authors.forename: P
authors.initials: P
authors.lastname: Torabi
authors.forename: M R
authors.initials: MR
authors.lastname: Shams Ardekani
authors.forename: M
authors.initials: M
authors.lastname: Hajimahmoodi
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003611:Dairy Products / Q000032:analysis*; D004032:Diet; D005520:Food Preservatives / Q000032:analysis*; D006801:Humans; D007492:Iran; D057230:Limit of Detection; D020160:Sodium Benzoate / Q000032:analysis*; D013011:Sorbic Acid / Q000032:analysis*
chemical_list: D005520:Food Preservatives; D020160:Sodium Benzoate; D013011:Sorbic Acid
keywords: HPLC; Iranian beverage; doogh; potassium sorbate; sodium benzoate