title: Optimisation and validation of analytical methods for the simultaneous extraction of antioxidants: application to the analysis of tomato sauces.
abstract: In the present study, simultaneous extraction of natural antioxidants (phenols and carotenoids) in complex matrices, such as tomato sauces, is presented. The tomato sauce antioxidant compounds studied were the phenolics hydroxytyrosol, from virgin olive oil, quercetin and its derivatives, from onions, and quercetin-rutinoside as well as the carotenoid, lycopene (cis and trans), from tomatoes. These antioxidant compounds were extracted simultaneously with n-hexane/acetone/ethanol (50/25/25, v/v/v). The phenolics were analysed by ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS), and lycopene (cis- and trans-forms) was analysed using high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). After studying the parameters of these methods, they were applied to the analysis of virgin olive oil, fresh onion, tomato concentrate and tomato powder, and commercial five tomato sauces. Subsequently, the results obtained in our laboratory were compared with those from the Gallina Blanca Star Group laboratory.
authors.affiliation: Food Technology Department, XaRTA-TPV, Universitat de Lleida-Agrotecnio Center, Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain. Electronic address: motilva@tecal.udl.es.
authors.forename: Maria-José
authors.initials: MJ
authors.lastname: Motilva
authors.affiliation: Food Technology Department, XaRTA-TPV, Universitat de Lleida-Agrotecnio Center, Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Macià
authors.affiliation: Food Technology Department, XaRTA-TPV, Universitat de Lleida-Agrotecnio Center, Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors.forename: Maria-Paz
authors.initials: MP
authors.lastname: Romero
authors.affiliation: Preparados Alimenticios S.A. subsidiary of Gallina Blanca Star Group, Plaza Europa 41, 08908 L'Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: Agustín
authors.initials: A
authors.lastname: Labrador
authors.affiliation: Preparados Alimenticios S.A. subsidiary of Gallina Blanca Star Group, Plaza Europa 41, 08908 L'Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Domínguez
authors.affiliation: Preparados Alimenticios S.A. subsidiary of Gallina Blanca Star Group, Plaza Europa 41, 08908 L'Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: Lluís
authors.initials: L
authors.lastname: Peiró
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000302:isolation & purification; D002338:Carotenoids / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D002338:Carotenoids; D000077276:Lycopene
keywords: Hydroxytyrosol; Lycopene; QTOF; Quercetin; Tandem MS; Tomato sauces