title: Effect of Camellia sinensis supplementation and increasing holding time on quality of cryopreserved boar semen.
abstract: Cryopreservation of boar semen is still considered suboptimal due to the low fertility when compared with fresh semen. This study was performed to evaluate the effects of green tea (Camellia sinensis) supplementation of the freezing extender at different concentration (0, 2.5%, 5%, 10%) and also to determine the influence of increasing holding time from 2 to 24 h at 15 °C. Seventeen ejaculates from nine boars were used to make pools of three of them and then cryopreserved. Sperm motility, viability, acrosome integrity, membrane functionality (HOST) and capacitation status were determined before freezing and at 0, 30, 60, 90 and 120 min after thawing. Lipid peroxidation was evaluated just after thawing. The main findings emerging from this study were the following: (i) no improvement in quality of thawed spermatozoa with addition of tea to the freezing extender, (ii) no improvement in quality of thawed spermatozoa with prolonged holding time, (iii) lower peroxidation rate in presence of tea 5% and (iv) a decrease in the number of uncapacited viable spermatozoa with any tea supplementation. We conclude that amplification of holding time in semen cryopreservation process does not vary results, facilitating freezing protocol. Tea supplementation reduces lipoxidation but did not improve quality parameters.
authors.affiliation: Department of Animal Pathology, Obstetrics and Reproduction Area, Faculty of Veterinary Medicine, Universidad de Zaragoza, Zaragoza, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Gale
authors.forename: L
authors.initials: L
authors.lastname: Gil
authors.forename: C
authors.initials: C
authors.lastname: Malo
authors.forename: N
authors.initials: N
authors.lastname: González
authors.forename: F
authors.initials: F
authors.lastname: Martínez
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D028241:Camellia sinensis; D015925:Cryopreservation / Q000379:methods*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D012661:Semen / Q000187:drug effects*; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D034421:Sus scrofa; D013997:Time Factors
chemical_list: D010936:Plant Extracts
keywords: Antioxidants; boar semen; cryopreservation; holding time