title: Hydroxyapatite grafted carbon nanotubes and graphene nanosheets: promising bone implant materials.
abstract: In the present study, hydroxyapatite (HA) was successfully grafted to carboxylated carbon nanotubes (CNTs) and graphene nanosheets. The HA grafted CNTs and HA-graphene nanosheets were characterized using FT-IR, TGA, SEM and X-ray diffraction. The HA grafted CNTs and graphene nanosheets (CNTs-HA and Gr-HA) were further used to examine the proliferation and differentiation rate of temperature-sensitive human fetal osteoblastic cell line (hFOB 1.19). Total protein assays and western blot analysis of osteocalcin expression were used as indicators of cell proliferation and differentiation. Results indicated that hFOB 1.19 cells proliferate and differentiate well in treatment media containing CNTs-HA and graphene-HA. Both CNTs-HA and graphene-HA could be promising nanomaterials for use as scaffolds in bone tissue engineering.
authors.affiliation: Department of Chemistry, Prairie View A&M University, Prairie View, TX 77446, United States.
authors.forename: Adebola
authors.initials: A
authors.lastname: Oyefusi
authors.affiliation: Department of Chemistry, Prairie View A&M University, Prairie View, TX 77446, United States.
authors.forename: Opeyemi
authors.initials: O
authors.lastname: Olanipekun
authors.affiliation: Department of Chemistry, Prairie View A&M University, Prairie View, TX 77446, United States.
authors.forename: Gururaj M
authors.initials: GM
authors.lastname: Neelgund
authors.affiliation: Cooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446, United States.
authors.forename: Deforest
authors.initials: D
authors.lastname: Peterson
authors.affiliation: Cooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446, United States.
authors.forename: Julia M
authors.initials: JM
authors.lastname: Stone
authors.affiliation: Cooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446, United States.
authors.forename: Ebonee
authors.initials: E
authors.lastname: Williams
authors.affiliation: Cooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446, United States.
authors.forename: Laura
authors.initials: L
authors.lastname: Carson
authors.affiliation: Department of Biology, Prairie View A&M University, Prairie View, TX 77446, United States.
authors.forename: Gloria
authors.initials: G
authors.lastname: Regisford
authors.affiliation: Department of Chemistry, Prairie View A&M University, Prairie View, TX 77446, United States. Electronic address: aroki@pvamu.edu.
authors.forename: Aderemi
authors.initials: A
authors.lastname: Oki
mesh_terms: D015153:Blotting, Western; D001842:Bone and Bones / Q000502:physiology*; D002460:Cell Line; D017886:Durapatite / Q000737:chemistry*; D005333:Fetus / Q000166:cytology; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology; D015675:Osteocalcin / Q000378:metabolism; D011208:Powders; D019736:Prostheses and Implants; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D037742:Nanotubes, Carbon; D011208:Powders; D015675:Osteocalcin; D006108:Graphite; D017886:Durapatite
keywords: Bioceramic; Carbon nanotubes; Graphene; Hydroxyapatite; hFOB 1.19 cells