title: Highly efficient folding of multi-disulfide proteins in superoxidizing Escherichia coli cytoplasm.
abstract: In this study, we monitored the thiol-disulfide redox potential of different Escherichia coli strains using redox-sensitive variants of green fluorescent protein. The cells with extreme oxidizing cytoplasm were generated by introducing a highly efficient disulfide relay system. The developed cells have exceptionally efficient de novo disulfide bond formation and significantly improve the oxidative folding of the client multi-disulfide proteins. Superoxidizing E. coli strain provides an effective method for the high-level production of recombinant disulfide-containing proteins.
authors.affiliation: Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
authors.forename: Wenyao
authors.initials: W
authors.lastname: Zhang
authors.forename: Wenyun
authors.initials: W
authors.lastname: Zheng
authors.forename: Miaowei
authors.initials: M
authors.lastname: Mao
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
mesh_terms: D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism*; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D050862:Oxidoreductases Acting on Sulfur Group Donors; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D004220:Disulfides; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D050862:Oxidoreductases Acting on Sulfur Group Donors
keywords: disulfide formation; oxidative protein folding; redox potential; superoxidizing cells