title: Influence of different test parameters on the microshear bond strength of two simplified etch-and-rinse adhesives.
abstract: OBJECTIVE
  To evaluate the effect of different test parameters on the resin-dentin microshear bond strength (μSBS).
  
  
  METHODS
  A 1.5-mm-thick dentin disk was prepared in each of 140 human molars. The disks were divided into five groups to test the following variables: time of adhesive light polymerization (n = 20), storage time (n = 40), bonding area (n = 40), Tygon tube removal (n = 20), and time of composite placement (n = 20). The adhesives were applied and each specimen was subjected to μSBS testing. All fractured specimens were observed with SEM. The data from each experiment were subjected to two-way ANOVA and Tukey's test (α = 0.05).
  
  
  RESULTS
  The storage time, bonding area, and Tygon tube removal did not influence the μSBS. Higher μSBS values were found when empty Tygon tubes were filled after positioning and when the adhesive was light polymerized before Tygon tube placement (p < 0.05).
  
  
  CONCLUSIONS
  Differences in test parameters affected the μSBS of adhesives, especially the time of adhesive light polymerization and composite placement.
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Andrade
authors.forename: Eugenio Jose
authors.initials: EJ
authors.lastname: Garcia
authors.forename: Farid S
authors.initials: FS
authors.lastname: El-Askary
authors.forename: Alessandra
authors.initials: A
authors.lastname: Reis
authors.forename: Alessandro D
authors.initials: AD
authors.lastname: Loguercio
authors.forename: Rosa Helena
authors.initials: RH
authors.lastname: Grande
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D003738:Dental Cements / Q000737:chemistry; D003764:Dental Materials / Q000737:chemistry; D003804:Dentin / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006801:Humans; D055115:Light-Curing of Dental Adhesives / Q000379:methods; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010756:Phosphoric Acids / Q000737:chemistry; D058105:Polymerization; D019279:Resin Cements / Q000737:chemistry*; D033081:Shear Strength; D013314:Stress, Mechanical; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: C554641:Adper single bond 2; D003188:Composite Resins; D003738:Dental Cements; D003764:Dental Materials; D017220:Dentin-Bonding Agents; C444701:Filtek Z250; D010756:Phosphoric Acids; D019279:Resin Cements; C532387:XP-Bond; D014867:Water; C030242:phosphoric acid