title: The role of Cysteine 227 in subcellular localization, water permeability, and multimerization of aquaporin-11.
abstract: Aquaporin-11 (AQP11) is the latest member of the mammalian water channel protein family to be described. Recent in vivo studies have shown that mutation at Cys(227) causes renal failure. However the importance of Cys(227) for the molecular function of AQP11 is largely unknown. In this study, we examined the subcellular localization, water permeability, and multimerization of AQP11 with a mutation at Cys(227). Interestingly, cells expressing the mutants had significantly higher osmotic water permeability. In contrast, the mutation lowered the cell surface expression and multimerization levels. Our observations suggest that Cys(227) is crucial for the proper molecular function of AQP11.
authors.affiliation: Department of Veterinary Pharmacology, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Saki
authors.initials: S
authors.lastname: Takahashi
authors.affiliation: Department of Veterinary Pharmacology, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Muta
authors.affiliation: Department of Veterinary Pharmacology, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Sonoda
authors.affiliation: Department of Veterinary Pharmacology, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Kato
authors.affiliation: Department of Veterinary Pharmacology, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abdeen
authors.affiliation: Department of Veterinary Pharmacology, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ikeda
keywords: AQP, aquaporin; Aqauaporin-11; DsRM, DsRed-Monomer; ER, endoplasmic reticulum; GFP, green fluorescent protein; Water channel; Water permeability