title: A hydrophobic disordered peptide spontaneously anchors a covalently bound RNA hairpin to giant lipidic vesicles.
abstract: The attraction of nucleic acids to lipidic compartments is the first step for carriers of potentially inheritable information to self-organise in functionalised synthetic cells. Confocal fluorescence imaging shows that a synthetic amphiphilic peptidyl RNA molecule spontaneously accumulates at the outer bilayer membranes of phospho- and glycolipidic giant vesicles. Cooperatively attractive interactions of -3.4 to -4.0 kcal mol(-1) between a random coil hydrophobic peptide and lipid membranes can thus pilot lipophobic RNA to its compartmentation. The separation of mixed lipid phases in the membranes further enhances the local concentration of anchored RNA.
authors.affiliation: Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (Unité Mixte de Recherche 5246), Université de Lyon, Claude Bernard Lyon 1, 69622 Villeurbanne Cedex, Lyon, France. strazewski@univ-lyon1.fr.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Le Chevalier Isaad
authors.forename: Paolo
authors.initials: P
authors.lastname: Carrara
authors.forename: Pasquale
authors.initials: P
authors.lastname: Stano
authors.forename: Kollappillil S
authors.initials: KS
authors.lastname: Krishnakumar
authors.forename: Dominique
authors.initials: D
authors.lastname: Lafont
authors.forename: Alexandra
authors.initials: A
authors.lastname: Zamboulis
authors.forename: René
authors.initials: R
authors.lastname: Buchet
authors.forename: Denis
authors.initials: D
authors.lastname: Bouchu
authors.forename: Florian
authors.initials: F
authors.lastname: Albrieux
authors.forename: Peter
authors.initials: P
authors.lastname: Strazewski
mesh_terms: D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D008563:Membrane Lipids; D010455:Peptides; D013501:Surface-Active Agents; D012313:RNA