title: Gold sputtered fiducial markers for combined secondary ion mass spectrometry and MALDI imaging of tissue samples.
abstract: Mass spectrometry imaging (MSI) is a label free technique capable of providing simultaneous identification and localization of biomolecules. A multimodal approach is required that allows for the study of the complexity of biological tissue samples to overcome the limitations of a single MSI technique. Secondary ion mass spectrometry (SIMS) allows for high spatial resolution imaging while matrix-assisted laser desorption (MALDI) offers a significantly wider mass range. The combination of coregistered SIMS and MALDI images results in detailed and unique biomolecular information. In this Technical Note, we describe how gold sputtered/implanted fiducial markers (FM) are created and can be used to ensure a proper overlay and coregistration of the two-dimensional images provided by the two MSI modalities.
authors.affiliation: Jozef Stefan Institute , Jamova 39, SI-1000 Ljubljana, Slovenia.
authors.forename: Nina
authors.initials: N
authors.lastname: Ogrinc Potočnik
authors.forename: Karolina
authors.initials: K
authors.lastname: Škrášková
authors.forename: Bryn
authors.initials: B
authors.lastname: Flinders
authors.forename: Primož
authors.initials: P
authors.lastname: Pelicon
authors.forename: Ron M A
authors.initials: RM
authors.lastname: Heeren
mesh_terms: D000818:Animals; D057918:Fiducial Markers; D006046:Gold; D006801:Humans; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*; D064726:Triple Negative Breast Neoplasms / Q000737:chemistry / Q000473:pathology
chemical_list: D006046:Gold