title: Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones.
abstract: CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6-yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P2₁, with unit-cell parameters a=70.0, b=104.8, c=73.5 Å, β=114.4°.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Dengfeng
authors.initials: D
authors.lastname: Yang
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Mori
authors.affiliation: Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsui
authors.affiliation: School of Pharmacy, Iwate Medical University, 2-1-1 Nishitokuta, Yahaba, Iwate, 028-3694, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hashimoto
authors.affiliation: Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.affiliation: School of Pharmacy, Iwate Medical University, 2-1-1 Nishitokuta, Yahaba, Iwate, 028-3694, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Fujii
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology*; D001483:Base Sequence; D003460:Crystallization; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D048630:Polyketide Synthases / Q000737:chemistry / Q000378:metabolism*; D011753:Pyrones / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D011753:Pyrones; D011994:Recombinant Proteins; D048630:Polyketide Synthases
keywords: Aspergillus oryzae; CsyB; chalcone synthase; csypyrone B1; polyketide synthase