title: Phytoextraction potential of wild type and 35S-gshI transgenic poplar trees (Populus x Canescens) for environmental pollutants herbicide paraquat, salt sodium, zinc sulfate and nitric oxide in vitro.
abstract: Phytoextraction potentials of two transgenic (TR) poplar (Populus x canescens) clones TRggs11 and TRlgl6 were compared with that of wild-type (WT) following exposure to paraquat, zinc sulfate, common salt and nitric oxide (NO), using a leaf-disc system incubated for 21 days on EDTA-containing nutritive WPM media in vitro. Glutathione (GSH) contents of leaf discs of TRlgl6 and TRggs11 showed increments to 296% and 190%, respectively, compared with WT. NO exposure led to a twofold GSH content in TRlgl6, which was coupled with a significantly increased sulfate uptake when exposed to 10(-3) M ZnSO4. The highest mineral contents of Na, Zn, Mn, Cu, and Mo was observed in the TRggs11 clone. Salt-induced activity of catalase enzyme increased in both TR clones significantly compared with WT under NaCl (0.75% and 1.5%) exposure. The in silico sequence analyses of gsh1 genes revealed that P. x canadensis and Salix sachalinensis show the closest sequence similarity to that of P. x canescens, which predicted an active GSH production with high phytoextraction potentials of these species with indication for their use where P. x canescens can not be grown.
authors.forename: G
authors.initials: G
authors.lastname: Gyulai
authors.forename: A
authors.initials: A
authors.lastname: Bittsánszky
authors.forename: Z
authors.initials: Z
authors.lastname: Szabó
authors.forename: L
authors.initials: L
authors.lastname: Waters
authors.forename: G
authors.initials: G
authors.lastname: Gullner
authors.forename: G
authors.initials: G
authors.lastname: Kampfl
authors.forename: G
authors.initials: G
authors.lastname: Heltai
authors.forename: T
authors.initials: T
authors.lastname: Komíves
mesh_terms: D001673:Biodegradation, Environmental; D002374:Catalase / Q000378:metabolism; D003553:Cystine / Q000032:analysis / Q000378:metabolism; D004785:Environmental Pollutants; D005838:Genotype; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D006540:Herbicides / Q000032:analysis / Q000378:metabolism*; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D010269:Paraquat / Q000032:analysis / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D032107:Populus / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000032:analysis / Q000378:metabolism*; D014197:Trees; D019287:Zinc Sulfate / Q000032:analysis / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D006540:Herbicides; D010940:Plant Proteins; D009569:Nitric Oxide; D012965:Sodium Chloride; D003553:Cystine; D019287:Zinc Sulfate; D002374:Catalase; D005978:Glutathione; D010269:Paraquat