title: Analysis of the released nuclear cytokine HMGB1 in human serum.
abstract: A ubiquitous nuclear protein, the high-mobility group box 1 (HMGB1), is secreted by activated macrophages/monocytes and leaked passively from injured cells. HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Here, we describe a semiquantitative immuno-blotting method to measure the released HMGB1 in human serum, in comparison with a commercially available HMGB1 ELISA technique.
authors.affiliation: Laboratory of Emergency Medicine, The Feinstein Institute for Medical Research, North Shore-LIJ Health System, 350 Community Drive, 11030, Manhasset, NY, USA, hwang@nshs.edu.
authors.forename: Haichao
authors.initials: H
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Shu
authors.initials: S
authors.lastname: Zhu
authors.forename: Maggie
authors.initials: M
authors.lastname: Yeung
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry; D015153:Blotting, Western / Q000379:methods / Q000592:standards*; D002467:Cell Nucleus / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D024243:HMGB1 Protein / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008262:Macrophage Activation; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D009195:Peroxidase / Q000737:chemistry
chemical_list: D000906:Antibodies; D024243:HMGB1 Protein; C585491:HMGB1 protein, human; D010455:Peptides; D009195:Peroxidase