title: Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge.
abstract: Mussel Mytilus galloprovincialis is a marine aquaculture shellfish distributing widely along the coast in north China. In this work, we studied the differential metabolic responses induced by Vibrio harveyi in digestive gland and gill tissues from M. galloprovincialis using NMR-based metabolomics. The differential metabolic responses in the two tissue types were detected, except the similarly altered taurine and betaine. These metabolic responses suggested that V. harveyi mainly induced osmotic disruption and reduced energy demand via the metabolic pathways of glucose synthesis and ATP/AMP conversion in mussel digestive gland. In mussel gill tissues, V. harveyi basically caused osmotic stress and possible reduced energy demand as shown by the elevated phosphocholine that is involved in one of the metabolic pathways of ATP synthesis from ADP and phosphocholine. The altered mRNA expression levels of related genes (superoxide dismutase with copper and zinc, heat shock protein 90, defensin and lysozyme) suggested that V. harveyi induced clear oxidative and immune stresses in both digestive gland and gill tissues. However, the mRNA expression levels of both lysozyme and defensin in digestive gland were more significantly up-regulated than those in gill from V. harveyi-challenged mussel M. galloprovincialis, meaning that the immune organ, digestive gland, was more sensitive than gill. Overall, our results indicated that V. harveyi could induce tissue-specific metabolic responses in mussel M. galloprovincialis.
authors.affiliation: School of Life Sciences, Ludong University, Yantai 264025, PR China. Electronic address: zlylxl2009@gmail.com.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory of Coastal Ecotoxicology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Ji
authors.affiliation: Laboratory of Coastal Ecotoxicology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Laboratory of Coastal Ecotoxicology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Laboratory of Coastal Ecotoxicology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Laboratory of Coastal Ecotoxicology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Huifeng
authors.initials: H
authors.lastname: Wu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017756:Aquaculture; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D005880:Gills / Q000382:microbiology; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome / Q000276:immunology*; D049878:Mytilus / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological / Q000276:immunology*; D014733:Vibrio / Q000276:immunology*
chemical_list: D017931:DNA Primers
keywords: Digestive gland; Gill; Metabolomics; Mytilus galloprovincialis; Vibrio harveyi