title: Investigating the effect of Phlomis lanceolata Boiss and hohen on cancer cell lines.
abstract: Phlomis lanceolata is a medicinal plant that has long been used to treat various conditions such as diabetes, gastric ulcer, hemorrhoids, inflammation and wounds. As most of Phlomis species have shown cytotoxic activity against proliferation of different cell lines, a biological investigation of P. lanceolata was carried out in this study. The aim of this study was to find out the in vitro cytotoxic activity of total extract and different fractions of Phlomis lanceolata on four cell lines. Cytotoxic activity of the metanolic total extract and partition fractions of chloroform, ethyl acetate and petroleum ether of flowering aerial parts of Phlomis lanceolata on the HT29, Caco2, T47D and NIH3T3 cell lines is examined by MTT. Petroleum ether fraction showed high cytotoxic activity against proliferation of all four cell lines. Presence of heavy triterpens and lipophil compounds recognized by TLC test in Petroleum ether fraction is responsible for high cytotoxic activity. The results emphasize the importance of phytochemical studies which could lead to the discovery of new active compounds.
authors.affiliation: Department of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran ostadnas@sina.tums.ac.ir.
authors.forename: Farnaz
authors.initials: F
authors.lastname: Soltani-Nasab
authors.affiliation: Department of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. ostadnas@sina.tums.ac.ir.
authors.forename: Jinous
authors.initials: J
authors.lastname: Asgarpanah
authors.affiliation: Department of Toxicology and Pharmacology, Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Virtual School, Tehran University of Medical Sciences, Tehran, Iran ostadnas@sina.tums.ac.ir.
authors.forename: Maryam
authors.initials: M
authors.lastname: Majdzadeh
authors.affiliation: Department of Toxicology, Pharmacology and Nanotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran ostadnas@sina.tums.ac.ir.
authors.forename: Seyed Nasser
authors.initials: SN
authors.lastname: Ostad
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D031345:Phlomis; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts