title: Ditopic boronic acid and imine-based naphthalimide fluorescence sensor for copper(II).
abstract: Copper ions are essential for many biological processes. However, high concentrations of copper can be detrimental to the cell or organism. A novel naphthalimide derivative bearing a monoboronic acid group (BNP) was investigated as a Cu(2+) selective fluorescent sensor in living cells. This derivative is one of the rare examples of reversible fluorescent chemosensors for Cu(2+) which uses a boronic acid group for a binding site. Moreover, the adduct BNP-Cu(2+) displays a fluorescence enhancement with fructose. The uptake of this novel compound in HeLa cancer cells was imaged using confocal fluorescence microscopy techniques including two-photon fluorescence lifetime imaging microscopy.
authors.affiliation: Department of Chemistry, University of Bath, Claverton Down, UK. t.d.james@bath.ac.uk s.pascu@bath.ac.uk.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Haobo
authors.initials: H
authors.lastname: Ge
authors.forename: Rory L
authors.initials: RL
authors.lastname: Arrowsmith
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Mirabello
authors.forename: Stanley W
authors.initials: SW
authors.lastname: Botchway
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhu
authors.forename: Sofia I
authors.initials: SI
authors.lastname: Pascu
authors.forename: Tony D
authors.initials: TD
authors.lastname: James
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007097:Imines / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods; D053644:Naphthalimides / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001897:Boronic Acids; D005456:Fluorescent Dyes; D007097:Imines; D053644:Naphthalimides; D003300:Copper