title: Expression of Wnt5a during development of anorectal malformations in a rat model of prenatal exposure to di(n-butyl) phthalate.
abstract: Mounting evidence has indicated the crucial role of Wnt5a in the embryonic development including guts. However, the Wnt5a involvement in the process of anorectal malformations (ARMs) remains unclear. In this study, we examined the expression of Wnt5a during ARMs development in the offspring of di(n-butyl) phthalate (DBP)-treated pregnant rats. During the neonatal period, Wnt5a expression was evaluated in the terminal rectum of ARM offspring, non-ARM littermates and controls. Using real-time polymerase chain reaction (real-time PCR), western-blot analysis and immunohistochemistry approaches, we found a significant decrease of Wnt5a expression in DBP-induced ARMs rats. Collectively, our results demonstrate the aberrant expression of Wnt5a during anorectal development, which suggests that Wnt5a might be involved in DBP-induced ARMs.
authors.affiliation: Department of Urology and.
authors.forename: En-Hui
authors.initials: EH
authors.lastname: Li
authors.forename: Bang-Min
authors.initials: BM
authors.lastname: Han
authors.forename: Wen-Lan
authors.initials: WL
authors.lastname: Sun
authors.forename: Sheng-Jie
authors.initials: SJ
authors.lastname: Liang
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Xia
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000071056:Anorectal Malformations; D001006:Anus, Imperforate / Q000139:chemically induced*; D015153:Blotting, Western; D003993:Dibutyl Phthalate / Q000633:toxicity*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D018811:Maternal Exposure; D023421:Models, Animal; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D003993:Dibutyl Phthalate
keywords: Anorectal malformations; Wnt5a; di(n-butyl) phthalate