title: Behavioural and biochemical stress responses of Palinurus elephas after exposure to boat noise pollution in tank.
abstract: This study examined the effects of boat noise on the behavioural and biochemical parameters of the Mediterranean spiny lobster (Palinurus elephas). The experiment was conducted in a tank equipped with a video and audio recording system. 18 experimental trials, assigned to boat noise and control conditions, were performed using lobsters in single and group of 4 specimens. After a 1h habituation period, we audio- and video-recorded the lobsters for 1h. During the experimental phase, the animals assigned to the boat groups were exposed to boat noise pollution (a random sequence of boat noises). Exposure to the noise produced significant variations in locomotor behaviours and haemolymphatic parameters. Our results indicate that the lobsters exposed to boat noises increased significantly their locomotor activities and haemolymphatic bioindicator of stressful conditions such as glucose, total proteins, Hsp70 expression and THC when tested both singly and in groups.
authors.affiliation: Istituto per l'Ambiente Marino Costiero U.O. di Capo Granitola, Consiglio Nazionale delle Ricerche, Via del Faro No. 3, 91021 Granitola, TP, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Filiciotto
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Viale delle Scienze;Edificio 16, Università degli Studi di Palermo, Italy.
authors.forename: Mirella
authors.initials: M
authors.lastname: Vazzana
authors.affiliation: Istituto per l'Ambiente Marino Costiero U.O. di Capo Granitola, Consiglio Nazionale delle Ricerche, Via del Faro No. 3, 91021 Granitola, TP, Italy. Electronic address: monica.celi@unipa.it.
authors.forename: Monica
authors.initials: M
authors.lastname: Celi
authors.affiliation: Istituto per l'Ambiente Marino Costiero U.O. di Capo Granitola, Consiglio Nazionale delle Ricerche, Via del Faro No. 3, 91021 Granitola, TP, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Maccarrone
authors.affiliation: Istituto per l'Ambiente Marino Costiero U.O. di Capo Granitola, Consiglio Nazionale delle Ricerche, Via del Faro No. 3, 91021 Granitola, TP, Italy; Department of Basic Sciences and Foundations, University of Urbino, Campus Scientifico 'Enrico Mattei', Urbino, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Ceraulo
authors.affiliation: Istituto per l'Ambiente Marino Costiero U.O. di Capo Granitola, Consiglio Nazionale delle Ricerche, Via del Faro No. 3, 91021 Granitola, TP, Italy.
authors.forename: Gaspare
authors.initials: G
authors.lastname: Buffa
authors.affiliation: Istituto per l'Ambiente Marino Costiero U.O. di Capo Granitola, Consiglio Nazionale delle Ricerche, Via del Faro No. 3, 91021 Granitola, TP, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Di Stefano
authors.affiliation: Istituto per l'Ambiente Marino Costiero U.O. di Capo Granitola, Consiglio Nazionale delle Ricerche, Via del Faro No. 3, 91021 Granitola, TP, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Mazzola
authors.affiliation: Istituto per l'Ambiente Marino Costiero U.O. di Capo Granitola, Consiglio Nazionale delle Ricerche, Via del Faro No. 3, 91021 Granitola, TP, Italy.
authors.forename: Giuseppa
authors.initials: G
authors.lastname: Buscaino
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D004787:Environmental Pollution; D008297:Male; D009622:Noise; D033521:Palinuridae; D012767:Ships
keywords: Acoustic emission; BOAT; BSA; Boat noise condition; CHH; CTRL; Control condition; DHC; Haemolymphatic stress; Hsp; Locomotor behaviour; Marine traffic; PBS; PC; SIM; Social Interaction Module; Spiny lobster; TBS; THC; Tris-buffered saline; Underwater noise pollution; bovine serum albumin; crustacean hyperglycaemic hormone; differential haemocyte count; heat shock protein; phosphate buffered saline; protein concentration; total haemocyte count