title: Cytoprotective silica coating of individual mammalian cells through bioinspired silicification.
abstract: The cytoprotective coating of physicochemically labile mammalian cells with a durable material has potential applications in cell-based sensors, cell therapy, and regenerative medicine, as well as providing a platform for fundamental single-cell studies in cell biology. In this work, HeLa cells in suspension were individually coated with silica in a cytocompatible fashion through bioinspired silicification. The silica coating greatly enhanced the resistance of the HeLa cells to enzymatic attack by trypsin and the toxic compound poly(allylamine hydrochloride), while suppressing cell division in a controlled fashion. This bioinspired cytocompatible strategy for single-cell coating was also applied to NIH 3T3 fibroblasts and Jurkat cells.
authors.affiliation: Center for Cell-Encapsulation Research and Molecular-Level Interface Research Center, Department of Chemistry, KAIST, Daejeon 305-701 (Korea) http://cisgroup.kaist.ac.kr.
authors.forename: Juno
authors.initials: J
authors.lastname: Lee
authors.forename: Jinsu
authors.initials: J
authors.lastname: Choi
authors.forename: Ji Hun
authors.initials: JH
authors.lastname: Park
authors.forename: Mi-Hee
authors.initials: MH
authors.lastname: Kim
authors.forename: Daewha
authors.initials: D
authors.lastname: Hong
authors.forename: Hyeoncheol
authors.initials: H
authors.lastname: Cho
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Yang
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
mesh_terms: D000818:Animals; D019610:Cytoprotection; D006367:HeLa Cells; D006801:Humans; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide
keywords: bioinorganic chemistry; cell coating; cytoprotection; nanostructures; silica