title: In vitro DNA binding studies of anticancer drug idarubicin using spectroscopic techniques.
abstract: The interaction between idarubicin and double stranded deoxyribonucleic acid (ds-DNA) was investigated by UV-VIS spectrophotometry, fluorescence and Raman spectroscopy techniques. The absorption spectra of idarubicin with ds-DNA showed a slight red shift and hypochromic effect. In the fluorescence experiments, emission peaks were decreased by adding ds-DNA. Using ethidium bromide (ETB) as a fluorescence probe, fluorescence quenching of the emission peak was observed in the ETB-DNA system when idarubicin was added. Moreover, similar results were obtained in Raman spectroscopy. Binding constants of idarubicin with ds-DNA were determined as 5.14×10(5) M(-1) and 5.8×10(5) M(-1) for UV-VIS spectrophotometry and fluorescence spectroscopy, respectively. The large binding constant indicated that idarubicin has a high affinity with ds-DNA. All the evidences indicated that the binding mode of idarubicin with DNA was an intercalative binding. Furthermore, quantitative determination of idarubicin in pharmaceutical formulation was done.
authors.affiliation: Gazi University, Faculty of Pharmacy, Department of Analytical Chemistry, 06330 Ankara, Turkey.
authors.forename: Can
authors.initials: C
authors.lastname: Ozluer
authors.affiliation: Gazi University, Faculty of Pharmacy, Department of Analytical Chemistry, 06330 Ankara, Turkey. Electronic address: eda@gazi.edu.tr.
authors.forename: Hayriye Eda Satana
authors.initials: HE
authors.lastname: Kara
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004996:Ethidium / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D015255:Idarubicin / Q000032:analysis / Q000737:chemistry*; D007700:Kinetics; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; C052890:ethidium-DNA complex; D004247:DNA; D004996:Ethidium; D015255:Idarubicin
keywords: DNA; DNA binding; Ethidium bromide; Idarubicin; Spectrofluorometry; Spectrophotometry