title: Transglycosylations employing recombinant α- and β-galactosidases and novel donor substrates.
abstract: Recombinant α- and β-galactosidases could be prepared in larger amounts for chemoenzymatic syntheses of glycosylated oligosaccharides relevant in nutrition approaches. α-Galactosidase RafA from Escherichia coli, another thermophilic α-galactosidase AgaB from Geobacillus stearothermophilus KVE39, and also a thermophilic β-galactosidase BglT from Thermus thermophilus TH 125 could be employed in α- and in β-glycosylations, respectively. With model structures as well as sucrose, isomaltitol, and isomaltulose the stereo- and regiospecificities were studied. Further, a number of modified donor structures with structural variation and different leaving groups were synthesized, employed, and compared to classical donors for these transglycosylations.
authors.affiliation: University of Hamburg, Faculty of Science, Department of Chemistry, Martin-Luther-King-Platz 6, D-20146 Hamburg, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Schröder
authors.affiliation: University of Hamburg, Faculty of Science, Department of Chemistry, Martin-Luther-King-Platz 6, D-20146 Hamburg, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Kröger
authors.affiliation: University of Stuttgart, Institute for Industrial Genetics, Allmandring 31, D-70569 Stuttgart, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Mattes
authors.affiliation: University of Hamburg, Faculty of Science, Department of Chemistry, Martin-Luther-King-Platz 6, D-20146 Hamburg, Germany. Electronic address: thiem@chemie.uni-hamburg.de.
authors.forename: Joachim
authors.initials: J
authors.lastname: Thiem
mesh_terms: D001411:Geobacillus stearothermophilus / Q000201:enzymology; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D007700:Kinetics; D011994:Recombinant Proteins / Q000378:metabolism*; D016963:Thermus thermophilus / Q000201:enzymology; D000519:alpha-Galactosidase / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D006027:Glycosides; D011994:Recombinant Proteins; D000519:alpha-Galactosidase; D001616:beta-Galactosidase
keywords: Chemoenzymatic glycosylation; Galactooligosaccharide synthesis; Recombinant galactosidases