title: The use of lysozyme modified with fluorescein for the detection of Gram-positive bacteria.
abstract: Lysozyme (1,4-β-N-acetylmuramidase) is commonly applied in the food, medical, and pharmaceutical industries. In this study, we tested a novel application of fluorescein-modified lysozyme (using carboxyfluorescein with a triazine-based coupling reagent) as a new tool for the detection of Gram-positive soil bacteria. The results, obtained by cultivation methods, fluorescence analysis, and laser interferometry, showed that, after optimization, fluorescein-modified lysozyme could be used to evaluate the prevalence of Gram-positive bacteria essential in bioremediation of soils with low pH, such as those degraded by sulfur.
authors.affiliation: Department of Microbiology, Jan Kochanowski University in Kielce, Świętokrzyska 15, 25-406 Kielce, Poland. Electronic address: arabski@ujk.edu.pl.
authors.forename: Michał
authors.initials: M
authors.lastname: Arabski
authors.affiliation: Department of Microbiology, Jan Kochanowski University in Kielce, Świętokrzyska 15, 25-406 Kielce, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Konieczna
authors.affiliation: Department of Microbiology, Jan Kochanowski University in Kielce, Świętokrzyska 15, 25-406 Kielce, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Tusińska
authors.affiliation: Department of Molecular Physics, Jan Kochanowski University, Świętokrzyska 15, 25-406 Kielce, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Wąsik
authors.affiliation: Institute of Organic Chemistry, Technical University of Lodz, Lodz, Poland.
authors.forename: Inga
authors.initials: I
authors.lastname: Relich
authors.affiliation: Institute of Organic Chemistry, Technical University of Lodz, Lodz, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Zając
authors.affiliation: Institute of Organic Chemistry, Technical University of Lodz, Lodz, Poland.
authors.forename: Zbigniew J
authors.initials: ZJ
authors.lastname: Kamiński
authors.affiliation: Department of Microbiology, Jan Kochanowski University in Kielce, Świętokrzyska 15, 25-406 Kielce, Poland.
authors.forename: Wiesław
authors.initials: W
authors.lastname: Kaca
mesh_terms: D004789:Enzyme Activation; D005456:Fluorescent Dyes / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D056265:Microbial Interactions; D009113:Muramidase / Q000378:metabolism*; D012988:Soil Microbiology; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D009113:Muramidase
keywords: Fluorescein; Gram positive bacteria; Laser interferometry; Lysozyme