title: Toxic pyrene metabolism in Mycobacterium gilvum PYR-GCK results in the expression of mammalian cell entry genes as revealed by transcriptomics study.
abstract: Mycobacterium gilvum PYR-GCK is a bacterial strain under study for its bioremediation use on heavy hydrocarbon pollutants in the environment. During the course of our study, mammalian cell entry (mce) genes, known to facilitate pathogenicity in M. tuberculosis, were highly expressed during a comparative and substrate-related cultural global transcriptomic study. RNA sequencing of the global transcriptome of the test strain in two different substrates, pyrene and glucose, showed high expression of the mce genes based on the differential results. After validating the expression of these genes with quantitative real-time PCR, we arrived at the conclusion that the genes were expressed based on the pyrene substrate (a phytosterol compound), and sterol metabolism is said to activate the expression of the mce genes in some actinomycetes bacteria, M. gilvum PYR-GCK in this case. This study is believed to be important based on the fact that some mycobacterial strains are undergoing a continuous research as a result of their use in practical bioremediation of anthropogenic exposure of toxic organic wastes in the environment.
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan 426-791, Republic of Korea.
authors.forename: Abimbola Comfort
authors.initials: AC
authors.lastname: Badejo
authors.forename: Won Hyong
authors.initials: WH
authors.lastname: Chung
authors.forename: Nam Shin
authors.initials: NS
authors.lastname: Kim
authors.forename: Se Kye
authors.initials: SK
authors.lastname: Kim
authors.forename: Jin Choul
authors.initials: JC
authors.lastname: Chai
authors.forename: Young Seek
authors.initials: YS
authors.lastname: Lee
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Jung
authors.forename: Hyo Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Young Gyu
authors.initials: YG
authors.lastname: Chai
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005810:Multigene Family; D009170:Nontuberculous Mycobacteria / Q000235:genetics* / Q000378:metabolism; D011721:Pyrenes / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D011721:Pyrenes; C030984:pyrene