title: Bimolecular fluorescent complementation (BiFC) by MAP kinases and MAPK phosphatases.
abstract: The adaptation of plants to the environment is a key property for survival. Adaptation responses to environmental cues are generated in cells by signaling initiated from cell receptors. Signal transduction is based on protein phosphorylation that is employed in mitogen-activated protein kinase (MAPK) cascades to integrate signals from receptors to cellular responses. MAPK activity is determined by phosphorylation of amino acid residues within the kinase activation loop and their dephosphorylation by phosphatases is essential to control signal duration and intensity.Monitoring protein-protein interactions (PPIs) of MAPKs with MAPK phosphatases in vivo provides valuable information about specificity and intracellular localization of the protein complex. Here, we report studying PPIs between Arabidopsis MAPKs and PP2C-type MAPK phosphatases using bimolecular fluorescent complementation (BiFC) in suspension cell protoplasts. The interactions of the MAPKs MPK3, MKP4 and MPK6 with the phosphatases AP2C1 and AP2C3 have been tested.
authors.affiliation: Max F. Perutz Laboratories, University and Medical University of Vienna, Dr. Bohrgasse 9, 1030, Vienna, Austria, alois.schweighofer@univie.ac.at.
authors.forename: Alois
authors.initials: A
authors.lastname: Schweighofer
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Shubchynskyy
authors.forename: Vaiva
authors.initials: V
authors.lastname: Kazanaviciute
authors.forename: Armin
authors.initials: A
authors.lastname: Djamei
authors.forename: Irute
authors.initials: I
authors.lastname: Meskiene
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics*; D008856:Microscopy, Fluorescence; D054639:Mitogen-Activated Protein Kinase Phosphatases / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011523:Protoplasts / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D008164:Luminescent Proteins; D020928:Mitogen-Activated Protein Kinases; D054639:Mitogen-Activated Protein Kinase Phosphatases