title: PNA-tagged peptide microarrays for ratiometric activity detection of cellular protein kinases.
abstract: Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.
authors.affiliation: Department of Applied Chemistry, Kyushu University.
authors.forename: Hiromu
authors.initials: H
authors.lastname: Ikeda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yayama
authors.forename: Akito
authors.initials: A
authors.lastname: Hata
authors.forename: Jumpei
authors.initials: J
authors.lastname: Kamimoto
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mori
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D010766:Phosphorylation; D040081:Protein Array Analysis / Q000379:methods*; D011494:Protein Kinases / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D020135:Peptide Nucleic Acids; D004247:DNA; D011494:Protein Kinases