title: Activities of three erythrocyte enzymes of hyperglycemic rats (Rattus norvegicus) treated with Allium sativa extract.
abstract: BACKGROUND
  The present study sought to investigate erythrocyte glutathione S-transferases (GST), NADH-Methaemoglobin reductase (NADH-MR) and Na(+)/K(+)-ATPase activities of hypoglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa as agent of glycemic control.
  
  
  METHODS
  Hyperglycemia was induced by a single intra-peritoneal injection of 0.1 mol/L alloxan monohydrate in phosphate buffer saline (PBS) solution (pH = 7.4); dosage = 140 mg/kg. At the end of the experimental time (t = 76 h), erythrocyte GST, NADH-MR and Na(+)/K(+)-ATPase activities as well as serum fasting blood sugar (FBS) levels were measured by spectrophotometric methods.
  
  
  RESULTS
  Serum FBS levels of control/normal (C/N) rats ranged between 72.93 ± 0.82-95.12 ± 0.92 mg/dL, whereas experimental rats without glycemic control gave: 249.41 ± 1.03-256.11 ± 1.23 mg/dL. Hyperglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa exhibited comparative reduced serum levels of FBS alongside with erythrocyte GST, NADH-MR and Na(+)/K(+)-ATPase activities. The average relative activities of the three enzymes and corresponding order of enzyme activity in hyperglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa was: NADH-MR = 60.99% > GST = 47.81% > Na(+)/K(+)-ATPase = 46.81%. In the same order, relative activities of the three enzymes in rats without glycemic control were: NADH-MR = 49.65% > GST = 23.69% > Na(+)/K(+)-ATPase = 17.02%.
  
  
  CONCLUSIONS
  Erythrocyte GST, NADH-MR and Na(+)/K(+)-ATPase activities gave insights into the pathophysiology of diabetic state and served as biomarkers for ascertaining therapeutic control in Type 1 diabetes mellitus.
authors.affiliation: Department of Biochemistry, Imo state university, Owerri 460222, Nigeria.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Chikezie
authors.affiliation: Department of Biochemistry, University of Port Harcourt, Port Harcourt 460222, Nigeria.
authors.forename: Augustine A
authors.initials: AA
authors.lastname: Uwakwe
keywords: Allium sativa; Diabetes mellitus; Glutathione S-transferases; Hyperglycemia; NADH-Methaemoglobin reductase; Na+/K+-ATPase