title: A pharmacoproteomic study confirms the synergistic effect of chondroitin sulfate and glucosamine.
abstract: Osteoarthritis (OA) is the most common age-related rheumatic disease. Chondrocytes play a primary role in mediating cartilage destruction and extracellular matrix (ECM) breakdown, which are main features of the OA joint. Quantitative proteomics technologies are demonstrating a very interesting power for studying the molecular effects of some drugs currently used to treat OA patients, such as chondroitin sulfate (CS) and glucosamine (GlcN). In this work, we employed the iTRAQ (isobaric tags for relative and absolute quantitation) technique to assess the effect of CS and GlcN, both alone and in combination, in modifying cartilage ECM metabolism by the analysis of OA chondrocytes secretome. 186 different proteins secreted by the treated OA chondrocytes were identified. 36 of them presented statistically significant differences (p ≤ 0.05) between untreated and treated samples: 32 were increased and 4 decreased. The synergistic chondroprotective effect of CS and GlcN, firstly reported by our group at the intracellular level, is now demonstrated also at the extracellular level.
authors.affiliation: 1] Servicio de Reumatología, Grupo de Proteómica, ProteoRed/ISCIII, INIBIC-Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain [2] Catedra Bioibérica-Universidade da Coruña. Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain.
authors.forename: Valentina
authors.initials: V
authors.lastname: Calamia
authors.affiliation: Servicio de Reumatología, Grupo de Proteómica, ProteoRed/ISCIII, INIBIC-Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Mateos
authors.affiliation: Servicio de Reumatología, Grupo de Proteómica, ProteoRed/ISCIII, INIBIC-Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Fernández-Puente
authors.affiliation: Servicio de Reumatología, Grupo de Proteómica, ProteoRed/ISCIII, INIBIC-Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Lourido
authors.affiliation: Servicio de Reumatología, Grupo de Proteómica, ProteoRed/ISCIII, INIBIC-Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Rocha
authors.affiliation: Servicio de Reumatología, Grupo de Proteómica, ProteoRed/ISCIII, INIBIC-Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Fernández-Costa
authors.affiliation: 1] Catedra Bioibérica-Universidade da Coruña. Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain [2] Pre-clinical R&D Area, Pharma Science Division, Bioibérica, Barcelona, Spain.
authors.forename: Eulalia
authors.initials: E
authors.lastname: Montell
authors.affiliation: 1] Catedra Bioibérica-Universidade da Coruña. Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain [2] Pre-clinical R&D Area, Pharma Science Division, Bioibérica, Barcelona, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Vergés
authors.affiliation: 1] Servicio de Reumatología, Grupo de Proteómica, ProteoRed/ISCIII, INIBIC-Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain [2] CIBER-BBN-Area de Terapia Celular. Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Ruiz-Romero
authors.affiliation: 1] Servicio de Reumatología, Grupo de Proteómica, ProteoRed/ISCIII, INIBIC-Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain [2] Catedra Bioibérica-Universidade da Coruña. Hospital Universitario A Coruña. C/As Xubias S/N. 15.006, A Coruña, Spain [3] Departamento de Medicina. Universidad de Santiago de Compostela. Santiago de Compostela, A Coruña, Spain [4] Red de Inflamación y Enfermedades Reumatológicas. RIER/ISCIII. Madrid. Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Blanco
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002358:Cartilage, Articular / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D004357:Drug Synergism; D005944:Glucosamine / Q000494:pharmacology*; D006801:Humans; D010003:Osteoarthritis / Q000188:drug therapy
chemical_list: D002809:Chondroitin Sulfates; D005944:Glucosamine