title: Absence of maternal cell contamination in mesenchymal stromal cell cultures derived from equine umbilical cord tissue.
abstract: This study aimed to determine whether maternal cell contamination exists in cells derived from equine umbilical cord tissue, a perspective material for cell-based therapies in veterinary medicine. Potential maternal cell contamination was analyzed at DNA level via a set of 16 microsatellite markers in cells originating from the cord tissue of 22 foals. In these cells no maternal cell contamination was detected at a sensitivity level of 0.01%. Our results suggest that equine umbilical cord tissue-derived cells are entirely of fetal origin.
authors.affiliation: Department of Biology of Reproduction, Institute of Animal Science, Prague, Czech Republic; Department of Cellular Neurophysiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. Electronic address: ivackova@seznam.cz.
authors.forename: I
authors.initials: I
authors.lastname: Vackova
authors.affiliation: Department of Molecular Genetics, Institute of Animal Science, Prague, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Czernekova
authors.affiliation: Department of Biology of Reproduction, Institute of Animal Science, Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Tomanek
authors.affiliation: Department of Animal Husbandry, Czech University of Life Sciences, Prague, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Navratil
authors.affiliation: Department of Biology of Reproduction, Institute of Animal Science, Prague, Czech Republic; Department of Cellular Neurophysiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: T
authors.initials: T
authors.lastname: Mosko
authors.affiliation: Department of Biology of Reproduction, Institute of Animal Science, Prague, Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Novakova
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006736:Horses; D059630:Mesenchymal Stem Cells; D018895:Microsatellite Repeats; D011247:Pregnancy; D014470:Umbilical Cord / Q000166:cytology*
keywords: Maternal cell contamination; Mesenchymal stromal cells; Microsatellite markers; Umbilical cord tissue