title: Metabolic and ventilatory effects of oral glucose load at rest and during incremental aerobic muscular work in young healthy adults.
abstract: We measured respiratory ratio (RR), pulmonary ventilation (VE) and end-tidal carbon dioxide partial pressure (ETPCO2) at rest and during cycling aerobic workloads (20%, 40%, 60% of estimated maximal oxygen uptake). Measurements were taken after overnight fasting and after an oral glucose load. RR, VE and ETPCO2 increased with workload. Glucose load caused RR and VE increments at rest (0.75 ± 0.01 vs. 0.86 ± 0.02, p < 0.01, and 10.8 ± 0.43 vs. 12.1 ± 0.49 l/min, p < 0.01, respectively) and for each workload (20% estimated maximal oxygen uptake: 0.77 ± 0.01 vs. 0.855 ± 0.02, p < 0.01, and 16.2 ± 0.73 vs. 17.7 ± 0.8 l/min, p < 0.01; 40% estimated maximal oxygen uptake: 0.76 ± 0.02 vs. 0.82 ± 0.01, p < 0.01, and 25.9 ± 1.1 vs. 28.3 ± 1.3 l/min, p < 0.05; 60% estimated maximal oxygen uptake: 0.85 ± 0.02 vs. 0.91 ± 0.02, p < 0.01, and 37.4 ± 1.7 vs. 40.9 ± 1.9 l/min, p < 0.05) but ETPCO2 did not change. The differences in RR before and after glucose load became smaller as the workload increased. Linear regression analysis of VE and carbon dioxide output yielded virtually identical results for both fasting and glucose load conditions. We have concluded that: a) for the metabolic carbon dioxide load increment due to glucose-induced RR increment, the physiological response is an increase of VE at all workloads. This response modulates constant ETPCO2 values; b) on workload increment, skeletal muscle increasingly utilises more and more glycogen stores, regardless of the blood glucose availability. This reduces the usefulness of dietary manipulations decreasing carbon dioxide metabolic load during muscular work in respiratory failure; c) the absolute value of metabolic carbon dioxide load exerts a role in ventilation regulation at rest and during aerobic exercise.
authors.affiliation: University of Padova Department of Biomedical Sciences Via Marzolo 3 35100 Padova Italy.
authors.forename: A
authors.initials: A
authors.lastname: Rubini
authors.affiliation: University of Padova Department of Biomedical Sciences Via Marzolo 3 35100 Padova Italy.
authors.forename: A
authors.initials: A
authors.lastname: Parmagnani
authors.affiliation: University of Padova Department of Biomedical Sciences Via Marzolo 3 35100 Padova Italy.
authors.forename: A
authors.initials: A
authors.lastname: Paoli
mesh_terms: D000284:Administration, Oral; D001642:Bicycling; D001786:Blood Glucose / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D015444:Exercise; D005947:Glucose / Q000008:administration & dosage* / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D064368:Healthy Volunteers; D006801:Humans; D016014:Linear Models; D008297:Male; D009119:Muscle Contraction; D053580:Muscle Strength; D018482:Muscle, Skeletal / Q000378:metabolism*; D010101:Oxygen Consumption; D010313:Partial Pressure; D012123:Pulmonary Ventilation; D013997:Time Factors; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D002245:Carbon Dioxide; D006003:Glycogen; D005947:Glucose
keywords: aerobic muscular work; carbon dioxide end-tidal pressure; carbon dioxide metabolic load; pulmonary ventilation; respiratory ratio