title: Extra virgin olive oil phenols suppress migration and invasion of T24 human bladder cancer cells through modulation of matrix metalloproteinase-2.
abstract: The consumption of extra virgin olive oil (EVOO), a common dietary habit of the Mediterranean people, seems to be related to a lower incidence of certain types of cancer including bladder neoplasm. Metastases are the major cause of bladder cancer-related deaths and targeting cell motility has been proposed as a therapeutic strategy to prevent cancer spread. This study aimed to investigate the potential antimetastatic effect of total phenols extracted from EVOO against the human transitional bladder carcinoma cell line T24. We also aimed at verifying that EVOO extract exerts cytotoxic effect on tumor cells without affecting normal urothelial fibroblasts. Our results show that EVOO extract can significantly inhibit the proliferation and motility of T24 bladder cells in a dose-dependent manner. In the same experimental conditions fibroblast proliferation and motility were not significantly modified. Furthermore the enzymatic activity of MMP-2 was inhibited at nontoxic EVOO extract doses only in T24 cells. The qRT-PCR revealed a decrease of the MMP-2 expression and a simultaneous increase of the tissue inhibitors of metalloproteinases expression. Our results may support the epidemiological evidences that link olive oil consumption to health benefits and may represent a starting point for the development of new anticancer strategies.
authors.affiliation: a Department of Medical-Surgical Sciences and Biotechnologies , "Sapienza" University of Rome , Latina , Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Coccia
authors.forename: Daniela
authors.initials: D
authors.lastname: Bastianelli
authors.forename: Luciana
authors.initials: L
authors.lastname: Mosca
authors.forename: Roberto
authors.initials: R
authors.lastname: Monticolo
authors.forename: Isabella
authors.initials: I
authors.lastname: Panuccio
authors.forename: Antonio
authors.initials: A
authors.lastname: Carbone
authors.forename: Antonella
authors.initials: A
authors.lastname: Calogero
authors.forename: Eugenio
authors.initials: E
authors.lastname: Lendaro
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D000069463:Olive Oil; D010636:Phenols / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2