title: Kinesin spindle protein inhibitors with diaryl amine scaffolds: crystal packing analysis for improved aqueous solubility.
abstract: Diaryl amine derivatives have been designed and synthesized as novel kinesin spindle protein (KSP) inhibitors based on planar carbazole-type KSP inhibitors with poor aqueous solubility. The new generation of inhibitors was found to show comparable inhibitory activity and high selectivity for KSP, and this was accompanied with improved solubility. Kinetic analysis and molecular modeling studies suggested that these inhibitors work in an ATP-competitive manner via binding to the secondary allosteric site formed by α4 and α6 helices of KSP. Comparative structural investigations on a series of compounds revealed that the higher solubility of diaryl amine-type inhibitors was attributed to fewer van der Waals interactions in the crystal packing and the hydrogen-bond acceptor nitrogen of the aniline moiety for favorable solvation.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyoto University , Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Takeuchi
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyoto University , Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Oishi
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyoto University , Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Kaneda
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyoto University , Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ohno
authors.affiliation: Faculty of Pharmacy, Kinki University , 3-4-1 Kowakae, Higashi-osaka 577-8502, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Faculty of Pharmacy, Kinki University , 3-4-1 Kowakae, Higashi-osaka 577-8502, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Nakanishi
authors.affiliation: Graduate School of Pharmaceutical Sciences, University of Shizuoka , Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Yamane
authors.affiliation: Graduate School of Pharmaceutical Sciences, University of Shizuoka , Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Sawada
authors.affiliation: Graduate School of Pharmaceutical Sciences, University of Shizuoka , Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Asai
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyoto University , Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Fujii
keywords: Aqueous solubility; crystal packing; diphenylamine; kinesin spindle protein