title: ELISA-based assay for IP-10 detection from filter paper samples.
abstract: IP-10 is a small pro-inflammatory chemokine secreted primarily from monocytes and fibroblasts. Alterations in IP-10 levels have been associated with inflammatory conditions including viral and bacterial infections, immune dysfunction, and tumor development. IP-10 is increasingly recognized as a biomarker that predicts severity of various diseases and can be used in the immunodiagnostics of Mycobacterium tuberculosis and cytomegalovirus infection. Here, we describe an ELISA-based method to detect IP-10 from dried blood and plasma spot samples.
authors.affiliation: Department of Pulmonary and Infectious Diseases, Copenhagen University Hospital of North Zealand, Hillerød, Denmark, camilla.h.drabe@gmail.com.
authors.forename: Camilla Heldbjerg
authors.initials: CH
authors.lastname: Drabe
authors.forename: Thomas
authors.initials: T
authors.lastname: Blauenfeldt
authors.forename: Morten
authors.initials: M
authors.lastname: Ruhwald
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015415:Biomarkers / Q000097:blood; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards*; D054357:Chemokine CXCL10 / Q000097:blood*; D059788:Dried Blood Spot Testing / Q000379:methods / Q000639:trends*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D000906:Antibodies; D015415:Biomarkers; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D011994:Recombinant Proteins; D006735:Horseradish Peroxidase