title: Proteomics analysis of Bacillus licheniformis in response to oligosaccharides elicitors.
abstract: The role of oligosaccharides as biotic elicitors has been recognised in the enhanced production of antibiotics from fungal and bacterial cultures. The yield of bacitracin A in cultures of Bacillus licheniformis was increased after supplementation with oligoguluronate (OG), and mannan oligosaccharides (MO) and its mechanism at transcription level been established already. However, the elicitation mechanism at post transcriptional level has not been reported so far. In this paper we investigate changes in proteomics of B. licheniformis in presence of the oligosaccharide elicitors OG and MO. Differentially expressed proteins were examined using 2D-PAGE stained with colloidal Coomassie and were further identified by LC-MS/MS. We identified 19 differentially expressed proteins including those involved in carbon metabolism, energy generation, amino acid biosynthesis, oxidative and general stress response. The novel findings of this work, together with previous reports, contribute to the unravelling of the overall mechanism of elicitation in B. licheniformis cultures and reliability of the use of these elicitors for potential industrial application.
authors.affiliation: Applied Biotechnology Research Group, Department of Molecular & Applied Biosciences, University of Westminster, London W1W 6UW, United Kingdom.
authors.forename: Patricia Fernanda
authors.initials: PF
authors.lastname: Reffatti
authors.affiliation: Applied Biotechnology Research Group, Department of Molecular & Applied Biosciences, University of Westminster, London W1W 6UW, United Kingdom.
authors.forename: Ipsita
authors.initials: I
authors.lastname: Roy
authors.affiliation: Applied Biotechnology Research Group, Department of Molecular & Applied Biosciences, University of Westminster, London W1W 6UW, United Kingdom.
authors.forename: Mark
authors.initials: M
authors.lastname: Odell
authors.affiliation: Applied Biotechnology Research Group, Department of Molecular & Applied Biosciences, University of Westminster, London W1W 6UW, United Kingdom. Electronic address: t.keshavarz@wmin.ac.uk.
authors.forename: Tajalli
authors.initials: T
authors.lastname: Keshavarz
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D001414:Bacitracin / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D007218:Industrial Microbiology; D053858:Metabolic Networks and Pathways; D009844:Oligosaccharides / Q000378:metabolism*; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D006603:Hexuronic Acids; D009844:Oligosaccharides; C028569:oligomannoside; D001414:Bacitracin; C007896:guluronic acid; D018698:Glutamic Acid; C026461:bacitracin A
keywords: Adaptation; Bacillus licheniformis; Biotic elicitation; Mechanism of action; Proteomics