title: C to U RNA editing mediated by APOBEC1 requires RNA-binding protein RBM47.
abstract: Cytidine (C) to Uridine (U) RNA editing is a post-transcriptional modification that is accomplished by the deaminase APOBEC1 and its partnership with the RNA-binding protein A1CF. We identify and characterise here a novel RNA-binding protein, RBM47, that interacts with APOBEC1 and A1CF and is expressed in tissues where C to U RNA editing occurs. RBM47 can substitute for A1CF and is necessary and sufficient for APOBEC1-mediated editing in vitro. Editing is further impaired in Rbm47-deficient mutant mice. These findings suggest that RBM47 and APOBEC1 constitute the basic machinery for C to U RNA editing.
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia Discipline of Medicine, Sydney Medical School University of Sydney, Sydney, NSW, Australia nfossat@cmri.org.au.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fossat
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia.
authors.forename: Karin
authors.initials: K
authors.lastname: Tourle
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia.
authors.forename: Tania
authors.initials: T
authors.lastname: Radziewic
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia.
authors.forename: Kristen
authors.initials: K
authors.lastname: Barratt
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia.
authors.forename: Doreen
authors.initials: D
authors.lastname: Liebhold
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia.
authors.forename: Joshua B
authors.initials: JB
authors.lastname: Studdert
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia.
authors.forename: Melinda
authors.initials: M
authors.lastname: Power
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Jones
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia Discipline of Medicine, Sydney Medical School University of Sydney, Sydney, NSW, Australia.
authors.forename: David A F
authors.initials: DA
authors.lastname: Loebel
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia Discipline of Medicine, Sydney Medical School University of Sydney, Sydney, NSW, Australia.
authors.forename: Patrick P L
authors.initials: PP
authors.lastname: Tam
mesh_terms: D000071479:APOBEC-1 Deaminase; D000818:Animals; D018938:Caco-2 Cells; D002467:Cell Nucleus / Q000378:metabolism; D003562:Cytidine / Q000378:metabolism; D003564:Cytidine Deaminase / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D008822:Mice, Transgenic; D021381:Protein Transport; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C585951:Rbm47 protein, mouse; D003562:Cytidine; D000071479:APOBEC-1 Deaminase; C000605089:APOBEC1 protein, human; C000605090:Apobec1 protein, mouse; D003564:Cytidine Deaminase; D014529:Uridine
keywords: APOBEC1; Apolipoprotein b; RBM47; RNA editing; intestine