title: Expression and localization of ghrelin and its functional receptor in corpus luteum during different stages of estrous cycle and the modulatory role of ghrelin on progesterone production in cultured luteal cells in buffalo.
abstract: Evidence obtained during recent years provided has insight into the regulation of corpus luteum (CL) development, function, and regression by locally produced ghrelin. The present study was carried out to evaluate the expression and localization of ghrelin and its receptor (GHS-R1a) in bubaline CL during different stages of the estrous cycle and investigate the role of ghrelin on progesterone (P4) production along with messenger RNA (mRNA) expression of P4 synthesis intermediates. The mRNA and protein expression of ghrelin and GHS-R1a was significantly greater in mid- and late luteal phases. Both factors were localized in luteal cells, exclusively in the cytoplasm. Immunoreactivity of ghrelin and GHS-R1a was greater during mid- and late luteal phases. Luteal cells were cultured in vitro and treated with ghrelin each at 1, 10, and 100 ng/mL concentrations for 48 h after obtaining 75% to 80% confluence. At a dose of 1 ng/mL, there was no significant difference in P4 secretion between control and treatment group. At 10 and 100 ng/mL, there was a decrease (P < 0.05) in P4 concentration, cytochrome P45011A1 (CYP11A1), and 3-beta-hydroxysteroid dehydrogenase mRNA expression and localization. There was no difference in mRNA expression of steroidogenic acute regulatory protein between control and treatment group. In summary, the present study provided evidence that ghrelin and its receptor are expressed in bubaline CL and are localized exclusively in the cell cytoplasm and ghrelin has an inhibitory effect on P4 production in buffalo.
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: M
authors.initials: M
authors.lastname: Gupta
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Dangi
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: V S
authors.initials: VS
authors.lastname: Chouhan
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: I
authors.initials: I
authors.lastname: Hyder
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: V
authors.initials: V
authors.lastname: Babitha
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: V P
authors.initials: VP
authors.lastname: Yadav
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville, FL 32611; D.H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, FL 32611.
authors.forename: F A
authors.initials: FA
authors.lastname: Khan
authors.affiliation: Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: A
authors.initials: A
authors.lastname: Sonwane
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: G
authors.initials: G
authors.lastname: Singh
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: G K
authors.initials: GK
authors.lastname: Das
authors.affiliation: Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: A
authors.initials: A
authors.lastname: Mitra
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: S
authors.initials: S
authors.lastname: Bag
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar 243122, India. Electronic address: msarkar24@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Sarkar
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002020:Buffaloes; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism; D003338:Corpus Luteum / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry; D030762:Estrous Cycle / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D054439:Ghrelin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054440:Receptors, Ghrelin / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D003470:Culture Media; D054439:Ghrelin; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D054440:Receptors, Ghrelin; D011374:Progesterone; D015096:3-Hydroxysteroid Dehydrogenases; D002786:Cholesterol Side-Chain Cleavage Enzyme
keywords: Buffalo; Corpus luteum; GHS-R1a; Ghrelin; Progesterone