title: Oxidizability assay of unfractionated plasma of patients' with different plasma profile: a methodological study.
abstract: BACKGROUND
  Present study describe the in vitro model of plasma oxidation of patients with different lipid profile, that can be correlated to their invivo plasma oxidizability in order to find the arterial diseases prone patient groups.
  
  
  METHODS
  The method applied here to measure the invitro plasma oxidizability, accounts a convenient way that can be well suited in any clinical laboratory settings. Un-fractionated plasma was exposed to CuSO4 (5.0 mmol/L), a pro-oxidant, and low frequency ultrasonic wave to induce oxidation, and finally oxidizability was calculated by TBARS and Conjugated Diene methods.
  
  
  RESULTS
  In our study, plasma LDL greater than 150 mg/dL possess 1.75 times more risk to undergo oxidation (CI, 0.7774 to 3.94; p = 0.071) than the low LDL plasma, percent of oxidation increased from 38.3% to 67.1% for the LDL level upto 150 mg/dL and high. Lag phase, which is considered as the plasma antioxidative protection, was also influenced by the higher LDL concentration. The mean lag time was 65.27 ± 20.02 (p = 0.02 compared to healthy), where as for 94.71 ± 35.11 min for the normolipidemic subject. The plasma oxidizability was also changed drastically for total cholesterol level, oxidative susceptibility shown 35% and 55.02% for 200 mg/dL and high respectively, however it didn't appear as risk factor. Patient samples were also stratified according to their age, gender, and blood glucose level. Older persons (≥40 years) were 1.096 times (95% CL, 0.5607 to 2.141, p = 0.396) than younger (≤39 years age), males are 1.071 (95% CI, 0.5072- 2.264) times than the females, and diabetic patients are 1.091 (CI, 0.6153 to 1.934, p = 0.391) times in more risk than the non-diabetic counterpart.
  
  
  CONCLUSIONS
  This method addressing its easy applicability in biomedical research. And by this we were able to show that patients with high LDL (≥150 mg/dL) are in alarming condition besides diabetic and elderly (≥40 years age) males are considered to be susceptible and more prone to develop vascular diseases.
authors.affiliation: Department of Pharmacy, Primeasia University, 9 Banani C/A, Dhaka 1213, Bangladesh.
authors.forename: Hasan
authors.initials: H
authors.lastname: Imam
authors.affiliation: Department of Pharmacy, Primeasia University, 9 Banani C/A, Dhaka 1213, Bangladesh.
authors.forename: Arfia
authors.initials: A
authors.lastname: Chowdhury
authors.affiliation: Department of Pharmacy, Primeasia University, 9 Banani C/A, Dhaka 1213, Bangladesh.
authors.forename: Nasir Uddin
authors.initials: NU
authors.lastname: Mahbub
authors.affiliation: Exim Bank Hospital, 840 Rokeya Soroni, Mirpur, Dhaka 1216, Bangladesh.
authors.forename: Amir
authors.initials: A
authors.lastname: Hossain
authors.affiliation: Department of Pharmacy, Primeasia University, 9 Banani C/A, Dhaka 1213, Bangladesh.
authors.forename: Mohammed Faisal Bin
authors.initials: MF
authors.lastname: Karim
authors.affiliation: Department of Pharmacy, Primeasia University, 9 Banani C/A, Dhaka 1213, Bangladesh.
authors.forename: Mohammad Burhan
authors.initials: MB
authors.lastname: Uddin
authors.affiliation: Clinical Investigation Centre, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Md Moklesur Rahman
authors.initials: MM
authors.lastname: Sarker
keywords: Conjugated diene; Plasma oxidation; TBARS; Type II Diabetes