title: The hnRNP-Q protein LIF2 participates in the plant immune response.
abstract: Eukaryotes have evolved complex defense pathways to combat invading pathogens. Here, we investigated the role of the Arabidopsis thaliana heterogeneous nuclear ribonucleoprotein (hnRNP-Q) LIF2 in the plant innate immune response. We show that LIF2 loss-of-function in A. thaliana leads to changes in the basal expression of the salicylic acid (SA)- and jasmonic acid (JA)- dependent defense marker genes PR1 and PDF1.2, respectively. Whereas the expression of genes involved in SA and JA biosynthesis and signaling was also affected in the lif2-1 mutant, no change in SA and JA hormonal contents was detected. In addition, the composition of glucosinolates, a class of defense-related secondary metabolites, was altered in the lif2-1 mutant in the absence of pathogen challenge. The lif2-1 mutant exhibited reduced susceptibility to the hemi-biotrophic pathogen Pseudomonas syringae and the necrotrophic ascomycete Botrytis cinerea. Furthermore, the lif2-1 sid2-2 double mutant was less susceptible than the wild type to P. syringae infection, suggesting that the lif2 response to pathogens was independent of SA accumulation. Together, our data suggest that lif2-1 exhibits a basal primed defense state, resulting from complex deregulation of gene expression, which leads to increased resistance to pathogens with various infection strategies. Therefore, LIF2 may function as a suppressor of cell-autonomous immunity. Similar to its human homolog, NSAP1/SYNCRIP, a trans-acting factor involved in both cellular processes and the viral life cycle, LIF2 may regulate the conflicting aspects of development and defense programs, suggesting that a conserved evolutionary trade-off between growth and defense pathways exists in eukaryotes.
authors.affiliation: INRA-AgroParisTech, UMR1318, Institut J.-P. Bourgin, Centre de Versailles-Grignon, Versailles, France.
authors.forename: Clémentine
authors.initials: C
authors.lastname: Le Roux
authors.affiliation: INRA-AgroParisTech, UMR1318, Institut J.-P. Bourgin, Centre de Versailles-Grignon, Versailles, France.
authors.forename: Stefania
authors.initials: S
authors.lastname: Del Prete
authors.affiliation: INRA-AgroParisTech, UMR1318, Institut J.-P. Bourgin, Centre de Versailles-Grignon, Versailles, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Boutet-Mercey
authors.affiliation: INRA-AgroParisTech, UMR1318, Institut J.-P. Bourgin, Centre de Versailles-Grignon, Versailles, France.
authors.forename: François
authors.initials: F
authors.lastname: Perreau
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan, France; CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan, France.
authors.forename: Claudine
authors.initials: C
authors.lastname: Balagué
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan, France; CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Roby
authors.affiliation: INRA-AgroParisTech, UMR1318, Institut J.-P. Bourgin, Centre de Versailles-Grignon, Versailles, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Fagard
authors.affiliation: INRA-AgroParisTech, UMR1318, Institut J.-P. Bourgin, Centre de Versailles-Grignon, Versailles, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Gaudin
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D020171:Botrytis; D003517:Cyclopentanes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D005961:Glucosinolates / Q000378:metabolism; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics; D044224:Pseudomonas syringae / Q000502:physiology; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics; D059467:Transcriptome / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005961:Glucosinolates; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C559923:LIF2 protein, Arabidopsis; D054883:Oxylipins; D016601:RNA-Binding Proteins; C011006:jasmonic acid; D020156:Salicylic Acid