title: [Effect of IL-17A on levels of antiheart autoantibodies in mice with viral myocarditis].
abstract: OBJECTIVE
  To explore the effect of interleukin-17A (IL-17A) on the serum level of antiheart autoantibodies in mice with viral myocarditis.
  
  
  METHODS
  Male wild-type (WT) and IL-17A-deficient (IL-17A(-/-)) BALB/c mice were intraperitoneally injected with Coxsackie virus B3 (CVB3) for establishing VMC models (VMC-WT group and VMC-IL-17A(-/-) group). Meanwhile, a control group (WT group) of WT mice were established by i.p. administration of phosphate buffered saline (PBS). Paraffin sections of cardiac tissues were made 14 days after CVB3 injection. Myocardial histopathologic changes were evaluated by HE staining. The levels of anti-adenine nucleotide translocator (ANT) autoantibody, anti-β-myosin heavy chain (β-MHC) autoantibody and anti-cardiac L-type calcium channel (CACH2) autoantibody in sera were measured by ELISA.
  
  
  RESULTS
  Compared with WT group, the levels of anti-ANT-autoantibody and anti-β-MHC-autoantibody significantly increased in VMC-WT group (P<0.01, P<0.05), while the concentration of anti-CACH2-autoantibody showed no significant difference between WT and VMC-WT groups (P>0.05). Compared with VMC-WT group, the level of anti-ANT-autoantibody was reduced in VMC-IL-17A(-/-) group (P<0.05), while the levels of anti-β-MHC-autoantibody and anti-CACH2-autoantibody showed no significant difference between them (P>0.05).
  
  
  CONCLUSIONS
  IL-17A contributed to the secretion of anti-ANT-autoantibody of VMC mice, but had no effect on the secretion of anti-β-MHC-autoantibody and anti-CACH2-autoantibody in VMC mice.
authors.affiliation: Department of Cardiology, Guangxi Institute of Cardiovascular Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Lai
authors.affiliation: Department of Cardiology, Guangxi Institute of Cardiovascular Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Cardiology, Guangxi Institute of Cardiovascular Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Cardiology, Guangxi Institute of Cardiovascular Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Kong
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D020746:Calcium Channels, L-Type / Q000276:immunology; D003384:Coxsackievirus Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D029822:Enterovirus B, Human / Q000276:immunology* / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D056945:Hep G2 Cells; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D000226:Mitochondrial ADP, ATP Translocases / Q000276:immunology; D009205:Myocarditis / Q000235:genetics / Q000276:immunology* / Q000821:virology; D009206:Myocardium / Q000276:immunology / Q000473:pathology; D018995:Myosin Heavy Chains / Q000276:immunology; D024501:Nonmuscle Myosin Type IIB / Q000276:immunology
chemical_list: D001323:Autoantibodies; D020746:Calcium Channels, L-Type; D020381:Interleukin-17; D000226:Mitochondrial ADP, ATP Translocases; D024501:Nonmuscle Myosin Type IIB; C103815:nonmuscle myosin type IIB heavy chain; D018995:Myosin Heavy Chains