title: The anti-aging and tumor suppressor protein Klotho enhances differentiation of a human oligodendrocytic hybrid cell line.
abstract: Klotho functions as an aging suppressor, which, in mice, extends lifespan when overexpressed and accelerates development of aging-like phenotypes when disrupted. Klotho is mainly expressed in brain and kidney and is secreted into the serum and CSF. We have previously shown that Klotho is reduced in brains of old monkeys, rats, and mice. We further reported the ability of Klotho to enhance oligodendrocyte differentiation and myelination. Here, we examined the signaling pathways induced by Klotho in MO3.13, a human oligodendrocytic hybrid cell line. We show that exogenous Klotho affects the ERK and Akt signaling pathways, decreases the proliferative abilities and enhances differentiation of MO3.13 cells. Furthermore, microarray analysis of Klotho-treated MO3.13 cells reveals a massive change in gene expression with 80 % of the differentially expressed genes being downregulated. Using gene set enrichment analysis, we predicted potential transcription factors involved in regulating Klotho-treated MO3.13 cells and found that these cells are highly enriched in the gene sets, that are similarly observed in cancer, cardiovascular disease, stress, aging, and hormone-related chemical and genetic perturbations. Since Klotho is downregulated in all brain tumors tested to date, enhancing Klotho has therapeutic potential for treating brain and other malignancies.
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, 72 E. Concord Street, K304, Boston, MA, 02118, USA.
authors.forename: Ci-Di
authors.initials: CD
authors.lastname: Chen
authors.affiliation: Department Biomedical Engineering, Boston University School of Medicine, Boston, MA, USA. cabraham@bu.edu.|Center for Individualized Medicine, Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, MN, 55905, USA. cabraham@bu.edu.
authors.forename: Hu
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Biology, Boston University School of Medicine, Boston, MA, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, 72 E. Concord Street, K304, Boston, MA, 02118, USA.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Hixson
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, 72 E. Concord Street, K304, Boston, MA, 02118, USA.
authors.forename: Ella
authors.initials: E
authors.lastname: Zeldich
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, 72 E. Concord Street, K304, Boston, MA, 02118, USA.|Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, Boston, MA, USA.
authors.forename: Carmela R
authors.initials: CR
authors.lastname: Abraham
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D005966:Glucuronidase / Q000494:pharmacology*; D006801:Humans; D000090265:Klotho Proteins; D020935:MAP Kinase Signaling System; D051379:Mice; D055495:Neurogenesis; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D014157:Transcription Factors; D005966:Glucuronidase; D000090265:Klotho Proteins