title: μHigh resolution-magic-angle spinning NMR spectroscopy for metabolic phenotyping of Caenorhabditis elegans.
abstract: Analysis of model organisms, such as the submillimeter-size Caenorhabditis elegans, plays a central role in understanding biological functions across species and in characterizing phenotypes associated with genetic mutations. In recent years, metabolic phenotyping studies of C. elegans based on (1)H high-resolution magic-angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy have relied on the observation of large populations of nematodes, requiring labor-intensive sample preparation that considerably limits high-throughput characterization of C. elegans. In this work, we open new platforms for metabolic phenotyping of C. elegans mutants. We determine rich metabolic profiles (31 metabolites identified) from samples of 12 individuals using a (1)H NMR microprobe featuring high-resolution magic-angle coil spinning (HR-MACS), a simple conversion of a standard HR-MAS probe to μHR-MAS. In addition, we characterize the metabolic variations between two different strains of C. elegans (wild-type vs slcf-1 mutant). We also acquire a NMR spectrum of a single C. elegans worm at 23.5 T. This study represents the first example of a metabolomic investigation carried out on a small number of submillimeter-size organisms, demonstrating the potential of NMR microtechnologies for metabolomics screening of small model organisms.
authors.affiliation: CEA Saclay, DSM, IRAMIS, UMR CEA/CNRS 3299-NIMBE, Laboratoire Structure et Dynamique par Résonance Magnétique, F-91191, Gif-sur-Yvette Cedex, France.
authors.forename: Alan
authors.initials: A
authors.lastname: Wong
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Li
authors.forename: Laurent
authors.initials: L
authors.lastname: Molin
authors.forename: Florence
authors.initials: F
authors.lastname: Solari
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Elena-Herrmann
authors.forename: Dimitris
authors.initials: D
authors.lastname: Sakellariou
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*