title: A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4.
abstract: Argonaute proteins are pivotal regulators of gene expression mediating miRNAs function. Modulating their activity would be extremely useful to elucidate the processes governing small-RNAs-guided gene silencing. We report the identification of a chemical compound able to compete with Argonaute 2 miRNAs binding, and we demonstrate that this functional inhibition determines effects similar to Argonaute 2 shRNA-mediated down-regulation, favoring granulocytic differentiation of the acute promyelocytic leukemia cell line NB4 in response to retinoic acid.
authors.affiliation: Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Section of Histology & Medical Embryology and ‡CNR-National Research Council of Italy, Institute of Molecular Biology and Pathology c/o Department of Biochemical Sciences ''A. Rossi Fanelli'', Sapienza University of Rome , 00161 Rome, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Masciarelli
authors.forename: Roberto
authors.initials: R
authors.lastname: Quaranta
authors.forename: Ilaria
authors.initials: I
authors.lastname: Iosue
authors.forename: Gianni
authors.initials: G
authors.lastname: Colotti
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Padula
authors.forename: Greta
authors.initials: G
authors.lastname: Varchi
authors.forename: Francesco
authors.initials: F
authors.lastname: Fazi
authors.forename: Alberto
authors.initials: A
authors.lastname: Del Rio
mesh_terms: D060565:Argonaute Proteins / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics / Q000473:pathology*; D035683:MicroRNAs / Q000378:metabolism*; D054852:Small Molecule Libraries; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; D035683:MicroRNAs; D054852:Small Molecule Libraries; D014212:Tretinoin