title: Protein kinase A regulates the osteogenic activity of Osterix.
abstract: Osterix belongs to the SP gene family and is a core transcription factor responsible for osteoblast differentiation and bone formation. Activation of protein kinase A (PKA), a serine/threonine kinase, is essential for controlling bone formation and BMP-induced osteoblast differentiation. However, the relationship between Osterix and PKA is still unclear. In this report, we investigated the precise role of the PKA pathway in regulating Osterix during osteoblast differentiation. We found that PKA increased the protein level of Osterix; PKA phosphorylated Osterix, increased protein stability, and enhanced the transcriptional activity of Osterix. These results suggest that Osterix is a novel target of PKA, and PKA modulates osteoblast differentiation partially through the regulation of Osterix.
authors.affiliation: College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Siyuan
authors.initials: S
authors.lastname: He
authors.forename: You Hee
authors.initials: YH
authors.lastname: Choi
authors.forename: Joong-Kook
authors.initials: JK
authors.lastname: Choi
authors.forename: Chang-Yeol
authors.initials: CY
authors.lastname: Yeo
authors.forename: ChangJu
authors.initials: C
authors.lastname: Chun
authors.forename: Kwang Youl
authors.initials: KY
authors.lastname: Lee
mesh_terms: D000818:Animals; D016723:Bone Remodeling / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology*; D010766:Phosphorylation; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; D014157:Transcription Factors; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: OSTEOBLAST DIFFERENTIATION; OSTERIX; PROTEIN KINASE A; PROTEIN STABILITY; TRANSCRIPTIONAL ACTIVITY