title: Germplasm preservation in vitro of Polygonum multiflorum Thunb.
abstract: BACKGROUND
  The root of Polygonum multiflorum Thunb. is a common traditional Chinese medicine. In recent years, the wild resources of P. multiflorum have been seriously broken, and the cultivated varieties have been degrading. The germplasm resources of P. multiflorum need protection and preservation. So far, no in vitro germplasm preservation of P. multiflorum has been reported.
  
  
  OBJECTIVE
  To explore a method for the in vitro germplasm preservation of P. multiflorum.
  
  
  METHODS
  A large number of buds from seed explants were induced by tissue culture. The single buds were used as experimental materials to study the effects of plant growth regulator, temperature, and osmotic pressure on the preservation time, growth recovery, and genetic stability.
  
  
  RESULTS
  When the buds were inoculated onto Murashige and Skoog (MS) basal media containing 4% w/v sucrose, 2% w/v mannitol, and 1% w/v sorbitol, supplemented with paclobutrazol (PP333) 1.0 mg/l, abscisic acid (ABA) 5.0 mg/l, and daminozide (B9) 30.0 mg/l in an illuminated chamber under a 16 h photoperiod of 1500 lx light intensity at 15°C for 10 months, the survival rate was over 70% with good growth recovery and genetic stability.
  
  
  CONCLUSIONS
  The results of this study can be used for medium-term in vitro germplasm preservation of P. multiflorum, and meeting actual needs of research and production.
authors.affiliation: Anhui Academy of Traditional Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei, Anhui, People's Republic of China ; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Huang
authors.affiliation: Anhui Academy of Traditional Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei, Anhui, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
authors.affiliation: Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Shan-Lin
authors.initials: SL
authors.lastname: Gao
authors.affiliation: Anhui Academy of Traditional Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei, Anhui, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
authors.affiliation: Anhui Academy of Traditional Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei, Anhui, People's Republic of China.
authors.forename: Dian-Lei
authors.initials: DL
authors.lastname: Wang
keywords: Germplasm; Polygonum multiflorum Thunb.; preservation in vitro