title: Cytotoxicity of soft denture lining materials depending on their component types.
abstract: OBJECTIVE
  To evaluate the difference in cytotoxicity of soft denture lining materials depending on their component types.
  
  
  METHODS
  Ten commercially available soft denture lining materials (SDLM) consisting of five silicone-based materials and five acrylic-based materials were evaluated. For the MTT test, cured SDLM samples were extracted in a culture medium for 24 hours, and L-929 cells were incubated in the extracted medium for 24 hours. Cell viability was determined using a microplate reader and compared with those of the negative control, which were cultured in a culture medium without test material. Agar overlay test was performed for the cured SDLM samples according to International Organization for Standardization (ISO) 7405.
  
  
  RESULTS
  Among silicone-based lining materials, GC Reline Soft, Mollosil plus, and Dentusil showed a cell viability of 107.2% ± 4.5%, 102.3% ± 2.84%, and 93.0% ± 8.0%, respectively, compared with the control. Mucopren and Sofreliner Tough displayed significantly lower cell viability (86.4% ± 10.3% and 81.5% ± 4.3%,respectively) compared with the control (P < .05). Among acrylic-based materials, Kooliner, Visco-gel, Soft liner, Dura Base, and Coe-Soft displayed cell viability of 99.2% ± 14.6%, 93.1% ± 9.5%, 89.1% ± 9.8%, 87.6% ± 7.9%, and 75.9% ± 15.7%, respectively, compared with the control. Dura Base and Coe-Soft displayed significantly lower cell viability compared to the control. However, for all tested materials, cell viability exceeded the requirement limit of 70% specified in ISO 10993-5. In the agar overlay test, all five silicone-based materials and acrylic-based Kooliner were ranked as "noncytotoxic." However, Visco-gel was ranked as "mildly cytotoxic," and Soft liner, Coe-Soft, and Dura Base were ranked as "moderately cytotoxic."
  
  
  CONCLUSIONS
  When an acrylic-based soft denture lining material is used, the possibility of a cytotoxic effect should be considered.
authors.forename: Yo Han
authors.initials: YH
authors.lastname: Song
authors.forename: Ho Jun
authors.initials: HJ
authors.lastname: Song
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Han
authors.forename: Hong So
authors.initials: HS
authors.lastname: Yang
authors.forename: Yeong Joon
authors.initials: YJ
authors.lastname: Park
mesh_terms: D000180:Acrylic Resins / Q000633:toxicity; D000362:Agar; D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D003470:Culture Media; D017077:Culture Media, Conditioned; D003764:Dental Materials / Q000633:toxicity*; D003822:Denture Liners; D005347:Fibroblasts / Q000187:drug effects; D008422:Materials Testing; D008689:Methacrylates / Q000633:toxicity; D008768:Methylmethacrylates / Q000633:toxicity; D051379:Mice; D019904:Polymethyl Methacrylate / Q000633:toxicity; D011145:Polyvinyls / Q000633:toxicity; D012826:Silicone Elastomers / Q000633:toxicity; D012833:Siloxanes / Q000633:toxicity; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; D004396:Coloring Agents; D003470:Culture Media; D017077:Culture Media, Conditioned; D003764:Dental Materials; D008689:Methacrylates; D008768:Methylmethacrylates; C483159:Mollosil Plus; D011145:Polyvinyls; D012826:Silicone Elastomers; D012833:Siloxanes; D013778:Tetrazolium Salts; D013844:Thiazoles; C034183:vinyl polysiloxane; C103363:kooliner; C069457:Coe-Soft denture lining material; D000362:Agar; C009931:poly(ethylmethacrylate); D019904:Polymethyl Methacrylate; C022616:thiazolyl blue