title: Polyphenols from blueberries modulate inflammation cytokines in LPS-induced RAW264.7 macrophages.
abstract: Polyphenols including 3-glucoside/arabinoside/galactoside-based polymers of delphinidins, petunidins, peonidins, malvidins and cyanidins are one type of biological macromolecules, which are extraordinarily rich in blueberries. Anti-inflammatory activity of blueberry polyphenols (BPPs) was investigated by using lipopolysaccharide (LPS) induced RAW264.7 macrophages. The results showed that BPPs suppressed the gene expression of IL-1β (interleukin-1β), IL-6 and IL-12p35. The inhibition effect on IL-1β and IL-6 mRNA was most obvious at the concentration of 10-200μg/mL BPPs. But the inhibition effect on IL-12p35 mRNA was increased with the increasing concentration of BPPs. When fixed at 100μg/mL BPPs, the most significant inhibition on IL-1β, IL-6 and IL-12p35 mRNA expression was detected at 12-48h. In conclusion, BPPs exhibit anti-inflammation activity by mediating and modulating the balances in pro-inflammatory cytokines of IL-1β, IL-6, and IL-12.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Institute of Agro-food Science and Technology, Shandong Academy of Agriculture Science, Jinan 250100, China.
authors.forename: Anwei
authors.initials: A
authors.lastname: Cheng
authors.affiliation: Institute of Agro-food Science and Technology, Shandong Academy of Agriculture Science, Jinan 250100, China.
authors.forename: Haiqing
authors.initials: H
authors.lastname: Yan
authors.affiliation: Institute of Agro-food Science and Technology, Shandong Academy of Agriculture Science, Jinan 250100, China.
authors.forename: Caijing
authors.initials: C
authors.lastname: Han
authors.affiliation: Institute of Agro-food Science and Technology, Shandong Academy of Agriculture Science, Jinan 250100, China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: yqtian@jiangnan.edu.cn.
authors.forename: Yaoqi
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Institute of Agro-food Science and Technology, Shandong Academy of Agriculture Science, Jinan 250100, China. Electronic address: chenxy@saas.ac.cn.
authors.forename: Xiangyan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D029798:Blueberry Plants / Q000737:chemistry*; D002460:Cell Line; D016207:Cytokines / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007249:Inflammation / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D059808:Polyphenols / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D059808:Polyphenols
keywords: Anti-inflammation; Blueberries; Polyphenols