title: OXA-207, a novel OXA-24 variant with reduced catalytic efficiency against carbapenems in Acinetobacter pittii from Spain.
abstract: A carbapenem-resistant Acinetobacter pittii strain carrying an OXA-24-like enzyme was isolated in northern Spain in 2008. Sequence analysis confirmed the presence of the novel bla(OXA-207) gene flanked by the site-specific XerC/XerD-like recombination binding sites and showing a unique Gly222Val substitution compared to OXA-24. Cloning and kinetic analysis showed that OXA-207 presents a reduction in the catalytic efficiency against carbapenems and a noticeable increase for oxacillin.
authors.affiliation: Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Spain rodrigocayosilva@gmail.com.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Cayô
authors.affiliation: Servicio de Microbiología, Complejo Hospitalario Universitario A Coruña (CHUAC)-Instituto de Investigación Biomédica de A Coruña (INIBIC), La Coruña, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Merino
authors.affiliation: Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Ruiz Del Castillo
authors.affiliation: Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Spain.
authors.forename: María Eliecer
authors.initials: ME
authors.lastname: Cano
authors.affiliation: Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Calvo
authors.affiliation: Servicio de Microbiología, Complejo Hospitalario Universitario A Coruña (CHUAC)-Instituto de Investigación Biomédica de A Coruña (INIBIC), La Coruña, Spain.
authors.forename: Germán
authors.initials: G
authors.lastname: Bou
authors.affiliation: Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Spain Departmento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Martínez-Martínez
mesh_terms: D000150:Acinetobacter / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001665:Binding Sites; D015780:Carbapenems / Q000378:metabolism / Q000494:pharmacology*; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial; D015870:Gene Expression; D006801:Humans; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010068:Oxacillin / Q000378:metabolism / Q000494:pharmacology*; D011995:Recombination, Genetic; D016415:Sequence Alignment; D013030:Spain; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D007527:Isoenzymes; D019426:Integrases; D001618:beta-Lactamases; C410345:beta-lactamase OXA-24; D010068:Oxacillin