title: Reactive oxygen species burst induced by aluminum stress triggers mitochondria-dependent programmed cell death in peanut root tip cells.
abstract: Recent studies had certified that aluminum (Al) induced ROS production and programmed cell death (PCD) in higher plants. The relationship between ROS production and PCD occurrence under Al stress is uncovered. The results showed that root elongation inhibition and PCD occurrence was induced by 100 μM AlCl3. Al stress induced ROS burst, up-regulated Rboh and COX gene expression, increased mitochondrial permeability transition pore (MPTP) opening, decreased inner mitochondrial membrane potential (ΔΨm), released cytochrome c from mitochondria to cytoplasm, activated caspase 3-like protease activity. Exogenous H2O2 aggravated the changes caused by Al and accelerated PCD occurrence, but ROS scavenger CAT and AsA reversed the changes caused by Al and inhibited PCD production. A potential cascade of cellular events during Al induced PCD via mitochondria dependent pathway and the mechanism of ROS on regulating PCD induced by Al is proposed.
authors.affiliation: College of Agronomy, Guang xi University, Da xue Road 100, Nanning 530004, PR China. Electronic address: alone77@qq.com.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Huang
authors.affiliation: College of Agronomy, Guang xi University, Da xue Road 100, Nanning 530004, PR China. Electronic address: yangxud2012@163.com.
authors.forename: Xudong
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Agronomy, Guang xi University, Da xue Road 100, Nanning 530004, PR China. Electronic address: yaoshaochang@163.com.
authors.forename: Shaochang
authors.initials: S
authors.lastname: Yao
authors.affiliation: College of Agronomy, Guang xi University, Da xue Road 100, Nanning 530004, PR China. Electronic address: tortoise41@gmail.com.
authors.forename: Thet
authors.initials: T
authors.lastname: LwinOo
authors.affiliation: College of Agronomy, Guang xi University, Da xue Road 100, Nanning 530004, PR China. Electronic address: wingtiger@gxaas.net.
authors.forename: Huyi
authors.initials: H
authors.lastname: He
authors.affiliation: College of Agronomy, Guang xi University, Da xue Road 100, Nanning 530004, PR China. Electronic address: waiqing1966@126.com.
authors.forename: Aiqin
authors.initials: A
authors.lastname: Wang
authors.affiliation: College of Agronomy, Guang xi University, Da xue Road 100, Nanning 530004, PR China. Electronic address: 821650255@qq.com.
authors.forename: Chuangzhen
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Agronomy, Guang xi University, Da xue Road 100, Nanning 530004, PR China. Electronic address: lfhe@gxu.edu.cn.
authors.forename: Longfei
authors.initials: L
authors.lastname: He
mesh_terms: D000535:Aluminum / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D010367:Arachis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D000535:Aluminum
keywords: Al toxicity; Mitochondria; Peanut; Programmed cell death; Reactive oxygen species burst