title: Interaction of Sesbania mosaic virus (SeMV) RNA-dependent RNA polymerase (RdRp) with the p10 domain of polyprotein 2a and its implications in SeMV replication.
abstract: Identification of viral encoded proteins that interact with RNA-dependent RNA polymerase (RdRp) is an important step towards unraveling the mechanism of replication. Sesbania mosaic virus (SeMV) RdRp was shown to interact strongly with p10 domain of polyprotein 2a and moderately with the protease domain. Mutational analysis suggested that the C-terminal disordered domain of RdRp is involved in the interaction with p10. Coexpression of full length RdRp and p10 resulted in formation of RdRp-p10 complex which showed significantly higher polymerase activity than RdRp alone. Interestingly, CΔ43 RdRp also showed a similar increase in activity. Thus, p10 acts as a positive regulator of RdRp by interacting with the C-terminal disordered domain of RdRp.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Kunduri
authors.initials: K
authors.lastname: Govind
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Arindam
authors.initials: A
authors.lastname: Bakshi
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Handanahal S
authors.initials: HS
authors.lastname: Savithri
keywords: 3AT, 3 amino-1,2,4 triazol; CP, coat protein; IPTG, isopropyl-1thio-β-d-galactopyranoside; LB, Luria Bertani broth; LacZ, β-galactosidase; MEL1, α-galactosidase; MP, movement protein; Ni–NTA, nickel–nitrilo tri-acetic acid; ONPG, ortho-nitrophenyl-β-galactoside; PBST, phosphate buffered saline with 0.1% TWEEN 20; Pro, protease; Protein-protein interactions; RNA-dependent RNA polymerase (RdRp); RdRp, RNA-dependent RNA polymerase; Replication; SD, synthetic dropout; SeMV, Sesbania mosaic virus; Sesbania mosaic virus; Sobemovirus; VPg, viral protein genome linked; Y2H, yeast two hybrid