title: LncRNA-regulated infection and inflammation pathways associated with pregnancy loss: genome wide differential expression of lncRNAs in early spontaneous abortion.
abstract: OBJECTIVE
  Spontaneous abortion (SA) occurs before 20 gestational weeks. Approximately, half of recurrent SA has no identifiable cause. No report has yet been investigated the possible involvement of lncRNA in pregnancy loss.
  
  
  METHODS
  Sixteen pairs of pregnancies with spontaneous abortions (SA) and induced abortions (IA) were studied. Embryonic sacs and decidua were collected for each pregnancy. A Human LncRNA Array was employed to profile genomewide lncRNAs, which were then validated by RT-PCR.
  
  
  RESULTS
  Differentially expressed lncRNAs were identified. Biological pathways were categorized into six major groups: infection and inflammation, metabolism, signaling and transcriptional regulation, smooth muscle contraction, cell process, and coagulation.
  
  
  CONCLUSIONS
  Infection and inflammation pathways regulated by lncRNAs were determined as the predominant pathogenetic factors underlying the SA. Finding that antisense lncRNAs have been either up- or down-regulated suggests that they may have both cis- and trans-regulations.
authors.affiliation: Shijiazhuang Obstetric and Maternal Health Hospital, Shijiazhuang, China; Chinese Alliance of Translational Medicine for Maternal and Children's Health, Shijiazhuang, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Qingying
authors.initials: Q
authors.lastname: Cao
authors.forename: Jun
authors.initials: J
authors.lastname: Ge
authors.forename: Chunmiao
authors.initials: C
authors.lastname: Liu
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Ma
authors.forename: Yuciu
authors.initials: Y
authors.lastname: Meng
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhao
authors.forename: Ru
authors.initials: R
authors.lastname: Liu
authors.forename: Caixia
authors.initials: C
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Julia
authors.initials: J
authors.lastname: Zhong
authors.forename: Weina
authors.initials: W
authors.lastname: Ju
authors.forename: Edmund C
authors.initials: EC
authors.lastname: Jenkins
authors.forename: W Ted
authors.initials: WT
authors.lastname: Brown
authors.forename: Nanbert
authors.initials: N
authors.lastname: Zhong
mesh_terms: D000028:Abortion, Induced; D000022:Abortion, Spontaneous / Q000235:genetics*; D001483:Base Sequence; D003656:Decidua / Q000166:cytology*; D057890:Epigenomics; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007249:Inflammation / Q000235:genetics* / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics*; D017423:Sequence Analysis, RNA; D015398:Signal Transduction / Q000235:genetics
chemical_list: D062085:RNA, Long Noncoding
keywords: Embryonic development; first trimester; long non-coding RNA (lncRNA); pathogenesis; pathways; spontaneous abortion