title: Novel magnesium alloy Mg-2La caused no cytotoxic effects on cells in physiological conditions.
abstract: Using several different in vitro assays, a new biodegradable magnesium alloy Mg-2La, composed of 98% magnesium and 2% lanthanum, was investigated as a possible implant material for biomedical applications. An in vitro cytotoxicity test, according to EN ISO 10993-5/12, with L929 and human osteoblastic cells identified no toxic effects on cell viability at physiological concentrations (at 50% dilutions and higher). The metabolic activity of human osteoblasts in the 100% extract was decreased to <70% and was therefore rated as cytotoxic. The degradation rates of Mg-2La were evaluated in phosphate buffered saline and four different cell culture media. The degradation rates were shown to be influenced by the composition of the solution, and the addition of fetal bovine serum slightly accelerated the corrosive process. The results of these in vitro experiments suggest that Mg-2La is a promising candidate for use as an orthopedic implant material.
authors.affiliation: Laboratory for Biomechanics and Biomaterials, Department of Orthopedic Surgery, Hannover Medical School, Anna-von-Borries-Straße 1-7, 30625 Hannover, Germany; CrossBIT, Center for Biocompatibility and Implant-Immunology, Department of Orthopedic Surgery, Hannover Medical School, Feodor-Lynen-Str. 31, 30625 Hannover. Electronic address: weizbauer.andreas@mh-hannover.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Weizbauer
authors.affiliation: Institute of Materials Science, Leibniz Universität Hannover, An der Universität 2, 30823 Garbsen, Germany.
authors.forename: Jan-Marten
authors.initials: JM
authors.lastname: Seitz
authors.affiliation: ABB AG, Trafoweg 4, 06112 Halle, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Werle
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Hegermann
authors.affiliation: Laboratory for Biomechanics and Biomaterials, Department of Orthopedic Surgery, Hannover Medical School, Anna-von-Borries-Straße 1-7, 30625 Hannover, Germany; CrossBIT, Center for Biocompatibility and Implant-Immunology, Department of Orthopedic Surgery, Hannover Medical School, Feodor-Lynen-Str. 31, 30625 Hannover.
authors.forename: Elmar
authors.initials: E
authors.lastname: Willbold
authors.affiliation: Institute of Materials Science, Leibniz Universität Hannover, An der Universität 2, 30823 Garbsen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Eifler
authors.affiliation: Laboratory for Biomechanics and Biomaterials, Department of Orthopedic Surgery, Hannover Medical School, Anna-von-Borries-Straße 1-7, 30625 Hannover, Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Windhagen
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Bünteweg 9, 30559 Hannover, Germany.
authors.forename: Janin
authors.initials: J
authors.lastname: Reifenrath
authors.affiliation: Laboratory for Biomechanics and Biomaterials, Department of Orthopedic Surgery, Hannover Medical School, Anna-von-Borries-Straße 1-7, 30625 Hannover, Germany.
authors.forename: Hazibullah
authors.initials: H
authors.lastname: Waizy
mesh_terms: D000497:Alloys / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003343:Corrosion; D006801:Humans; D007811:Lanthanum / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry*; D051379:Mice; D009994:Osmolar Concentration; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D000497:Alloys; D012710:Serum Albumin, Bovine; D007811:Lanthanum; D008274:Magnesium
keywords: Cytotoxicity; Degradation; In vitro; Magnesium alloy; Orthopedic