title: Versatile types of polysaccharide-based supramolecular polycation/pDNA nanoplexes for gene delivery.
abstract: Different polysaccharide-based supramolecular polycations were readily synthesized by assembling multiple β-cyclodextrin-cored star polycations with an adamantane-functionalized dextran via host-guest interaction in the absence or presence of bioreducible linkages. Compared with nanoplexes of the starting star polycation and pDNA, the supramolecular polycation/pDNA nanoplexes exhibited similarly low cytotoxicity, improved cellular internalization and significantly higher gene transfection efficiencies. The incorporation of disulfide linkages imparted the supramolecular polycation/pDNA nanoplexes with the advantage of intracellular bioreducibility, resulting in better gene delivery properties. In addition, the antitumor properties of supramolecular polycation/pDNA nanoplexes were also investigated using a suicide gene therapy system. The present study demonstrates that the proper assembly of cyclodextrin-cored polycations with adamantane-functionalized polysaccharides is an effective strategy for the production of new nanoplex delivery systems.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers (Beijing University of Chemical Technology), Ministry of Education, Beijing Laboratory of Biomedical Materials, College of Materials Science & Engineering, Beijing University of Chemical Technology, Beijing 100029, China. xufj@mail.buct.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.forename: Nana
authors.initials: N
authors.lastname: Zhao
authors.forename: Bingran
authors.initials: B
authors.lastname: Yu
authors.forename: Fusheng
authors.initials: F
authors.lastname: Liu
authors.forename: Fu-Jian
authors.initials: FJ
authors.lastname: Xu
mesh_terms: D000218:Adamantane / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity; D019556:COS Cells; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D005437:Flucytosine / Q000737:chemistry / Q000633:toxicity; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000633:toxicity; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011134:Polysaccharides / Q000737:chemistry*; D014162:Transfection; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D000218:Adamantane; D000970:Antineoplastic Agents; D005437:Flucytosine; D049452:Green Fluorescent Proteins; D011073:Polyamines; D000071228:Polyelectrolytes; D011134:Polysaccharides; D047392:beta-Cyclodextrins; C485184:enhanced green fluorescent protein; C031215:betadex