title: Construction of an electrode modified with gallium(III) for voltammetric detection of ovalbumin.
abstract: Electrodes modified with gallium(III) complexes were constructed to detect ovalbumin (OVA). For immobilization of a gallium(III)-nitrilotriacetate (NTA) complex, the electrode was first covered with collagen film. After the amino groups of the film had reacted with isothiocyanobenzyl-NTA, the gallium(III) was then able to combine with the NTA moieties. Another design featured an electrode cast with a gallium(III)-acetylacetonate (AA) complex. The amount of gallium(III) in the NTA complex was equivalent to one-quarter of the gallium(III) that could be utilized from an AA complex. However, the calibration curves of OVA using gallium(III)-NTA and gallium(III)-AA complexes were linear in the ranges of 7.0 × 10(-11) - 3.0 × 10(-9) M and 5.0 × 10(-10) - 8.0 × 10(-9) M, respectively. The gallium(III) on the electrode with NTA complex had high flexibility due to the existence of a spacer between the NTA and the collagen film, and, therefore, the reactivity of the gallium(III) to OVA was superior to that of the gallium(III)-AA complex with no spacer.
authors.affiliation: Maebashi Institute of Technology.
authors.forename: Kazuharu
authors.initials: K
authors.lastname: Sugawara
authors.forename: Makoto
authors.initials: M
authors.lastname: Okusawa
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Takano
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Kadoya
mesh_terms: D055664:Electrochemical Techniques; D004566:Electrodes; D005708:Gallium / Q000737:chemistry*; D010047:Ovalbumin / Q000032:analysis* / Q000737:chemistry; D010766:Phosphorylation
chemical_list: D010047:Ovalbumin; D005708:Gallium