title: Improved MALDI-TOF microbial mass spectrometry imaging by application of a dispersed solid matrix.
abstract: The key step in high quality microbial matrix-assisted laser desorption/ionization mass spectrometry imaging (microbial MALDI MSI) is the fabrication of a homogeneous matrix coating showing a fine-grained morphology. This application note addresses a novel method to apply solid MALDI matrices onto microbial cultures grown on thin agar media. A suspension of a mixture of 2,5-DHB and α-CHCA is sprayed onto the agar sample surface to form highly homogeneous matrix coatings. As a result, the signal intensities of metabolites secreted by the fungus Aspergillus fumigatus were found to be clearly enhanced.
authors.affiliation: Institute of Organic Chemistry, University of Innsbruck, 6020, Innsbruck, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Vergeiner
authors.forename: Lukas
authors.initials: L
authors.lastname: Schafferer
authors.forename: Hubertus
authors.initials: H
authors.lastname: Haas
authors.forename: Thomas
authors.initials: T
authors.lastname: Müller
mesh_terms: D000085:Acetates / Q000737:chemistry; D001232:Aspergillus fumigatus / Q000254:growth & development / Q000378:metabolism*; D002725:Chloroform / Q000737:chemistry; D003373:Coumaric Acids / Q000737:chemistry*; D005841:Gentisates / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry*; D008752:Methylene Chloride / Q000737:chemistry; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D010316:Particle Size; D011422:Propionates; D012997:Solvents / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014835:Volatilization
chemical_list: D000085:Acetates; D003373:Coumaric Acids; D005841:Gentisates; D007202:Indicators and Reagents; D011422:Propionates; D012997:Solvents; D008752:Methylene Chloride; D002725:Chloroform; C495469:p-coumaric acid; C010925:2,5-dihydroxybenzoic acid; C046923:methyl acetate