title: Relationships between androgens, serotonin gene expression and innervation in male macaques.
abstract: Androgen administration to castrated individuals was purported to decrease activity in the serotonin system. However, we found that androgen administration to castrated male macaques increased fenfluramine-induced serotonin release as reflected by increased prolactin secretion. In this study, we sought to define the effects of androgens and aromatase inhibition on serotonin-related gene expression in the dorsal raphe, as well as serotonergic innervation of the LC. Male Japanese macaques (Macaca fuscata) were castrated for 5-7 months and then treated for 3 months with (1) placebo, (2) testosterone (T), (3) dihydrotestosterone (DHT; non-aromatizable androgen) and ATD (steroidal aromatase inhibitor), or (4) Flutamide (FLUT; androgen antagonist) and ATD (n=5/group). This study reports the expression of serotonin-related genes: tryptophan hydroxylase 2 (TPH2), serotonin reuptake transporter (SERT) and the serotonin 1A autoreceptor (5HT1A) using digoxigenin-ISH and image analysis. To examine the production of serotonin and the serotonergic innervation of a target area underlying arousal and vigilance, we measured the serotonin axon density entering the LC with ICC and image analysis. TPH2 and SERT expression were significantly elevated in T- and DHT + ATD-treated groups over placebo- and FLUT + ATD-treated groups in the dorsal raphe (p < 0.007). There was no difference in 5HT1A expression between the groups. There was a significant decrease in the pixel area of serotonin axons and in the number of varicosities in the LC across the treatment groups with T > placebo > DHT + ATD = FLUT + ATD treatments. Comparatively, T- and DHT + ATD-treated groups had elevated TPH2 and SERT gene expression, but the DHT + ATD group had markedly suppressed serotonin axon density relative to the T-treated group. Further comparison with previously published data indicated that TPH2 and SERT expression reflected yawning and basal prolactin secretion. The serotonin axon density in the LC agreed with the area under the fenfluramine-stimulated prolactin curve, providing a morphological basis for the pharmacological results. This suggested that androgen activity increased TPH2 and SERT gene expression but, aromatase activity, and neural production of estradiol (E), may subserve axonal serotonin and determination of the compartment acted upon by fenfluramine. In summary, androgens stimulated serotonin-related gene expression, but aromatase inhibition dissociated gene expression from the serotonin innervation of the LC terminal field and fenfluramine-stimulated prolactin secretion.
authors.affiliation: Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR 97006, United States; Division of Neuroscience, Oregon National Primate Research Center, Beaverton, OR 97006, United States; Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, OR 97201, United States. Electronic address: betheac@ohsu.edu.
authors.forename: C L
authors.initials: CL
authors.lastname: Bethea
authors.affiliation: Behavioral Sciences Unit, Division of Comparative Medicine, Oregon National Primate Research Center, Beaverton, OR 97006, United States.
authors.forename: K
authors.initials: K
authors.lastname: Coleman
authors.affiliation: Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR 97006, United States.
authors.forename: K
authors.initials: K
authors.lastname: Phu
authors.affiliation: Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR 97006, United States.
authors.forename: A P
authors.initials: AP
authors.lastname: Reddy
authors.affiliation: Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR 97006, United States.
authors.forename: A
authors.initials: A
authors.lastname: Phu
mesh_terms: D000726:Androgen Antagonists / Q000494:pharmacology; D000728:Androgens / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D047072:Aromatase Inhibitors / Q000494:pharmacology; D001370:Axonal Transport / Q000187:drug effects / Q000502:physiology; D001369:Axons / Q000187:drug effects / Q000378:metabolism*; D002369:Castration; D013196:Dihydrotestosterone / Q000008:administration & dosage / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005277:Fenfluramine / Q000494:pharmacology; D005485:Flutamide / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D008125:Locus Coeruleus / Q000187:drug effects / Q000378:metabolism*; D008251:Macaca; D008297:Male; D011388:Prolactin / Q000378:metabolism; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism*; D018490:Serotonin Agents / Q000494:pharmacology; D050486:Serotonin Plasma Membrane Transport Proteins / Q000378:metabolism*; D013739:Testosterone / Q000008:administration & dosage / Q000378:metabolism; D014365:Tryptophan Hydroxylase / Q000378:metabolism*
chemical_list: D000726:Androgen Antagonists; D000728:Androgens; D047072:Aromatase Inhibitors; D018490:Serotonin Agents; D050486:Serotonin Plasma Membrane Transport Proteins; D013196:Dihydrotestosterone; D044282:Receptor, Serotonin, 5-HT1A; D005277:Fenfluramine; D013739:Testosterone; D004958:Estradiol; D005485:Flutamide; D011388:Prolactin; D001141:Aromatase; D014365:Tryptophan Hydroxylase
keywords: 5HT1A; SERT; TPH2; androgen; aromatase; serotonin