title: A correlation study of the expression of resistin and glycometabolism in muscle tissue after traumatic brain injury in rats.
abstract: OBJECTIVE
  To investigate the expression pattern of resistin (RSTN) in skeletal muscle tissue and its influence on glycometabolism in rats with traumatic brain injury (TBI).
  
  
  METHODS
  Seventy-eight SD rats were randomly divided into traumatic group (n=36), RSTN group (n=36) and sham operation group (n=6). Fluid percussion TBI model was developed in traumatic and RSTN groups and the latter received additional 1 mg RSTN antibody treatment for each rat. At respectively 12 h, 24 h, 72 h, 1 w, 2 w, and 4 w after operation, venous blood was collected and the right hind leg skeletal muscle tissue was sampled. We used real-time PCR to determine mRNA expression of RSTN in skeletal muscles, western blot to determine RSTN protein expression and ELISA to assess serum insulin as well as fasting blood glucose (FBG) levels. Calculation of the quantitative insulin sensitivity check index (Q value) was also conducted. The above mentioned indicators and their correction were statistically analyzed.
  
  
  RESULTS
  Compared with sham operation group, the RSTN expression in the skeletal muscle as well as serum insulin and FBG levels revealed significant elevation (P<0.05), and reduced Q value (P<0.05) in traumatic group. Single factor linear correlation analysis showed a significant negative correlation between RSTN expression and Q values (P<0.001) in traumatic group.
  
  
  CONCLUSIONS
  The expression of RSTN has been greatly increased in the muscular tissue of TBI rats and it was closely related to the index of glycometabolism. RSTN may play an important role in the process of insulin resistance after TBI.
authors.affiliation: Emergency Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.forename: Lielie
authors.initials: L
authors.lastname: Zhu
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Zhang
authors.forename: Songling
authors.initials: S
authors.lastname: Xie
authors.forename: Da
authors.initials: D
authors.lastname: Pan
authors.forename: Hao
authors.initials: H
authors.lastname: Wen
authors.forename: Weiyang
authors.initials: W
authors.lastname: Meng
authors.forename: Luyang
authors.initials: L
authors.lastname: Lin
authors.forename: Daqing
authors.initials: D
authors.lastname: Chen
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007333:Insulin Resistance; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D052243:Resistin / Q000032:analysis*
chemical_list: D052243:Resistin; D005947:Glucose