title: Isolation, cultivation and characterisation of pigeon osteoblasts seeded on xenogeneic demineralised cancellous bone scaffold for bone grafting.
abstract: Avian osteoblasts have been isolated particularly from chicken embryo, but data about other functional tissue sources of adult avian osteoblast precursors are missing. The method of preparation of pigeon osteoblasts is described in this study. We demonstrate that pigeon cancellous bone derived osteoblasts have particular proliferative capacity in vitro in comparison to mammalian species and developed endogenous ALP. Calcium deposits formation in vitro was confirmed by alizarin red staining. Only a few studies have attempted to investigate bone grafting and treatment of bone loss in birds. Lack of autologous bone grafts in birds has prompted investigation into the use of avian xenografts for bone augmentation. Here we present a method of xenografting of ostrich demineralised cancellous bone scaffold seeded with allogeneic adult pigeon osteoblasts. Ostrich demineralised cancellous bone scaffold supported proliferation of pigeon osteoblasts during two weeks of co - cultivation in vitro. Scanning electron microscopy demonstrated homogeneous adult pigeon osteoblasts attachment and distribution on the surface of xenogeneic ostrich demineralised cancellous bone. Our preliminary in vitro results indicate that demineralised cancellous bone from ostrich tibia could provide an effective biological support for growth and proliferation of allogeneic osteoblasts derived from cancellous bone of pigeons.
authors.affiliation: Associated Tissue Bank of Faculty of Medicine of P. J. Safárik University and L. Pasteur University Hospital, Trieda SNP 1, SK-041 66, Kosice, Slovakia, denisa.harvanova@upjs.sk.
authors.forename: Denisa
authors.initials: D
authors.lastname: Harvanová
authors.forename: Slavomír
authors.initials: S
authors.lastname: Hornák
authors.forename: Judita
authors.initials: J
authors.lastname: Amrichová
authors.forename: Tímea
authors.initials: T
authors.lastname: Spaková
authors.forename: Jaromír
authors.initials: J
authors.lastname: Mikes
authors.forename: Jana
authors.initials: J
authors.lastname: Plsíková
authors.forename: Valent
authors.initials: V
authors.lastname: Ledecký
authors.forename: Ján
authors.initials: J
authors.lastname: Rosocha
mesh_terms: D000818:Animals; D016025:Bone Transplantation / Q000662:veterinary*; D018929:Cell Culture Techniques / Q000662:veterinary*; D002478:Cells, Cultured; D010856:Columbidae; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D020310:Struthioniformes; D054457:Tissue Scaffolds / Q000662:veterinary