title: Relationship between the v₂PO₄/amide III ratio assessed by Raman spectroscopy and the calcium content measured by quantitative backscattered electron microscopy in healthy human osteonal bone.
abstract: Raman microspectroscopy and quantitative backscattered electron imaging (qBEI) of bone are powerful tools to investigate bone material properties. Both methods provide information on the degree of bone matrix mineralization. However, a head-to-head comparison of these outcomes from identical bone areas has not been performed to date. In femoral midshaft cross sections of three women, 99 regions (20×20 μm²) were selected inside osteons and interstitial bone covering a wide range of matrix mineralization. As the focus of this study was only on regions undergoing secondary mineralization, zones exhibiting a distinct gradient in mineral content close to the mineralization front were excluded. The same regions were measured by both methods. We found a linear correlation (R²=0.75) between mineral/matrix as measured by Raman spectroscopy and the wt. %Mineral/(100-wt. %Mineral) as obtained by qBEI, in good agreement with theoretical estimations. The observed deviations of single values from the linear regression line were determined to reflect biological heterogeneities. The data of this study demonstrate the good correspondence between Raman and qBEI outcomes in describing tissue mineralization. The obtained correlation is likely sensitive to changes in bone tissue composition, providing an approach to detect potential deviations from normal bone.
authors.affiliation: Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Straße 30, A-1140 Vienna, AustriabMax Planck Institute of Colloids and Interfaces, 14424 Pot.
authors.forename: Andreas
authors.initials: A
authors.lastname: Roschger
authors.affiliation: Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Straße 30, A-1140 Vienna, Austria.
authors.forename: Sonja
authors.initials: S
authors.lastname: Gamsjaeger
authors.affiliation: Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Straße 30, A-1140 Vienna, Austria.
authors.forename: Birgit
authors.initials: B
authors.lastname: Hofstetter
authors.affiliation: Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
authors.forename: Admir
authors.initials: A
authors.lastname: Masic
authors.affiliation: Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Straße 30, A-1140 Vienna, Austria.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Blouin
authors.affiliation: Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Straße 30, A-1140 Vienna, Austria.
authors.forename: Phaedra
authors.initials: P
authors.lastname: Messmer
authors.affiliation: Medical University of Vienna, Department of Forensic Medicine, A-1090 Vienna, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Berzlanovich
authors.affiliation: Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Straße 30, A-1140 Vienna, Austria.
authors.forename: Eleftherios P
authors.initials: EP
authors.lastname: Paschalis
authors.affiliation: Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Straße 30, A-1140 Vienna, Austria.
authors.forename: Paul
authors.initials: P
authors.lastname: Roschger
authors.affiliation: Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Straße 30, A-1140 Vienna, Austria.
authors.forename: Klaus
authors.initials: K
authors.lastname: Klaushofer
authors.affiliation: Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Fratzl
mesh_terms: D000328:Adult; D000577:Amides / Q000737:chemistry*; D015519:Bone Density; D001842:Bone and Bones / Q000473:pathology*; D002118:Calcium / Q000737:chemistry*; D005260:Female; D005269:Femur / Q000473:pathology; D006801:Humans; D008854:Microscopy, Electron; D008875:Middle Aged; D055095:Optics and Photonics; D010710:Phosphates / Q000737:chemistry*; D012044:Regression Analysis; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012815:Signal Processing, Computer-Assisted; D013059:Spectrum Analysis, Raman
chemical_list: D000577:Amides; D010710:Phosphates; D002118:Calcium