title: Synthesis and functional characterization of a fluorescent peptide probe for non invasive imaging of collagen in live tissues.
abstract: Targeted molecular imaging to detect changes in the structural and functional organization of tissues, at the molecular level, is a promising approach for effective and early diagnosis of diseases. Quantitative and qualitative changes in type I collagen, which is a major component in the extra cellular matrix (ECM) of skin and other vital organs like lung, liver, heart and kidneys, are often associated with the pathophysiology of these organs. We have synthesized a fluorescent probe that comprises collagelin, a specific collagen binding peptide, coupled to fluorescent porphyrin that can effectively detect abnormal deposition of collagen in live tissues by emitting fluorescence in the near infra red (NIR) region. In this report we have presented the methodology for coupling of 5-(4-carboxy phenyl)-10, 15, 20-triphenyl porphyrin (C-TPP) to the N-terminal of collagelin or to another mutant peptide (used as a control). We have evaluated the efficacy of these fluorescent peptides to detect collagen deposition in live normal and abnormal tissues. Our results strongly suggest that porphyrin-tagged collagelin can be used as an effective probe for the non invasive in vivo detection of tissue fibrosis, especially in the liver.
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
authors.forename: Ushasri
authors.initials: U
authors.lastname: Chilakamarthi
authors.affiliation: CSIR-Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500007, India.
authors.forename: Jaipal
authors.initials: J
authors.lastname: Kandhadi
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Gunda
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
authors.forename: Avinash Raj
authors.initials: AR
authors.lastname: Thatipalli
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Kumar Jerald
authors.affiliation: CSIR-Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500007, India.
authors.forename: Giribabu
authors.initials: G
authors.lastname: Lingamallu
authors.affiliation: CSIR-Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500007, India.
authors.forename: Rakesh C
authors.initials: RC
authors.lastname: Reddy
authors.affiliation: CSIR-Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500007, India.
authors.forename: Arabinda
authors.initials: A
authors.lastname: Chaudhuri
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India. Electronic address: gpande@ccmb.res.in.
authors.forename: Gopal
authors.initials: G
authors.lastname: Pande
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D005355:Fibrosis / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D057054:Molecular Imaging / Q000379:methods*; D010455:Peptides / Q000378:metabolism*; D011166:Porphyrins / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D011166:Porphyrins; D003094:Collagen
keywords: Diagnostic probe; Liver fibrosis; Porphyrin; Type I collagen