title: Salidroside protects Caenorhabditis elegans neurons from polyglutamine-mediated toxicity by reducing oxidative stress.
abstract: Polyglutamine (polyQ) aggregation plays a pivotal role in the pathological process of Huntington's disease and other polyQ disorders. Therefore, strategies aiming at restoring dysfunction and reducing stresses mediated by polyQ toxicity are of therapeutic interest for proteotoxicity diseases. Salidroside, a glycoside from Rhodiola rosea, has been shown to have a variety of bioactivities, including antioxidant activity. Using transgenic Caenorhabditis elegans models, we show here that salidroside is able to reduce neuronal death and behavioral dysfunction mediated by polyQ expressed in ASH neurons, but the neuroprotective effect is not associated with prevention of polyQ aggregation per se. Further experiments reveal that the neuroprotective effect of salidroside in C. elegans models involves its antioxidant capabilities, including decrease of ROS levels and paraquat-induced mortality, increase of antioxidant enzyme activities and reduction of lipid peroxidation. These results demonstrate that salidroside exerts its neuroprotective function against polyQ toxicity via oxidative stress pathways.
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China. xiaolingyun@whu.edu.cn.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Xiao
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China. lhf@whu.edu.cn.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China. haz0709@163.com.
authors.forename: Ju
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China. yangfan6616@126.com.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China. haz0709@163.com.
authors.forename: Aizhen
authors.initials: A
authors.lastname: Huang
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China. cjhsusan@whu.edu.cn.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Deng
authors.affiliation: Research & Development Centre, Infinitus (China) Company Ltd, Guangzhou 510665, China. fiona.liang@infinitus-int.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Liang
authors.affiliation: Research & Development Centre, Infinitus (China) Company Ltd, Guangzhou 510665, China. mary.ma@infinitus-int.com.
authors.forename: Fangli
authors.initials: F
authors.lastname: Ma
authors.affiliation: Research & Development Centre, Infinitus (China) Company Ltd, Guangzhou 510665, China. mandy.hu@infinitus-int.com.
authors.forename: Minghua
authors.initials: M
authors.lastname: Hu
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China. zebohuang@gdpu.edu.cn.
authors.forename: Zebo
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology*; D005960:Glucosides / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D010455:Peptides / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology*
chemical_list: D005960:Glucosides; D010455:Peptides; D010636:Phenols; C097188:polyglutamine; C009172:rhodioloside