title: Antagonistic interactions of "Ya-Sa-Marn-Phlae" ethanol extract in combination with topical antiseptics against clinical isolates of Staphylococcus aureus.
abstract: This investigation was aimed at assessing a possible interaction of a traditional Thai herbal recipe, "Ya-Sa-Marn-Phlae (YSMP)," used for wound treatments with topical antiseptics, povidone-iodine (PI) solution and hydrogen peroxide (H2O2), and effects of THR-SK010 alone and the combinations on Staphylococcus aureus. Antibacterial activities of ethanol extracts from the herbal recipe were determined against both methicillin resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA). YSMP exhibited remarkable antistaphylococcal activity with MIC values of 3.9-7.8 μg/mL. This recipe possessed bacteriostatic activity and did not reduce the tolerance of both MRSA and MSSA isolates to the high ionic strength. Interaction between THR-SK010 and the antiseptics was carried out by checkerboard testing and time-kill assay. Both indifferent and slightly antagonistic effects were observed with THR-SK010/PI and THR-SK010/H2O2 combinations against the tested isolates. In addition to commercially available antiseptics, THR-SK010 offered additional therapeutic options for the decolonization of MRSA and MSSA. Topical application of plant extracts with antioxidant activity, such as THR-SK010, should not be used immediately with PI or H2O2 and further investigation on this interaction is needed.
authors.affiliation: Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkla 90110, Thailand ; Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkla 90110, Thailand.
authors.forename: Sasitorn
authors.identifier: 0000-0002-7365-6890
authors.initials: S
authors.lastname: Chusri
authors.affiliation: Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkla 90110, Thailand.
authors.forename: Sirirat
authors.initials: S
authors.lastname: Tongrod
authors.affiliation: Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkla 90110, Thailand ; Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors.forename: Julalak
authors.initials: J
authors.lastname: Chokpaisarn
authors.affiliation: Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkla 90110, Thailand ; Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkla 90110, Thailand.
authors.forename: Surasak
authors.initials: S
authors.lastname: Limsuwan
authors.affiliation: Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkla 90110, Thailand ; Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors.forename: Supayang Piyawan
authors.initials: SP
authors.lastname: Voravuthikunchai
mesh_terms: D000287:Administration, Topical; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C000601040:Ya-Sa-Marn-Phlae; D000431:Ethanol