title: In Silico Prediction of T and B Cell Epitopes of Der f 25 in Dermatophagoides farinae.
abstract: The house dust mites are major sources of indoor allergens for humans, which induce asthma, rhinitis, dermatitis, and other allergic diseases. Der f 25 is a triosephosphate isomerase, representing the major allergen identified in Dermatophagoides farinae. The objective of this study was to predict the B and T cell epitopes of Der f 25. In the present study, we analyzed the physiochemical properties, function motifs and domains, and structural-based detailed features of Der f 25 and predicted the B cell linear epitopes of Der f 25 by DNAStar protean system, BPAP, and BepiPred 1.0 server and the T cell epitopes by NetMHCIIpan-3.0 and NetMHCII-2.2. As a result, the sequence and structure analysis identified that Der f 25 belongs to the triosephosphate isomerase family and exhibited a triosephosphate isomerase pattern (PS001371). Eight B cell epitopes (11-18, 30-35, 71-77, 99-107, 132-138, 173-187, 193-197, and 211-224) and five T cell epitopes including 26-34, 38-54, 66-74, 142-151, and 239-247 were predicted in this study. These results can be used to benefit allergen immunotherapies and reduce the frequency of mite allergic reactions.
authors.affiliation: Department of Allergy, First Affiliated Hospital of Anhui Medical University, No. 210, Jixi Road, Anhui Province, Hefei 230022, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Urology, First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, China.
authors.forename: Hai-Wei
authors.initials: HW
authors.lastname: Yang
authors.affiliation: Research Division of Clinical Pharmacology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Allergy, First Affiliated Hospital of Anhui Medical University, No. 210, Jixi Road, Anhui Province, Hefei 230022, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Allergy, First Affiliated Hospital of Anhui Medical University, No. 210, Jixi Road, Anhui Province, Hefei 230022, China.
authors.forename: Yehai
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Research Division of Clinical Pharmacology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.
authors.forename: Ji-Fu
authors.identifier: 0000-0001-6412-3251
authors.initials: JF
authors.lastname: Wei