title: Chitosan microsphere scaffold tethered with RGD-conjugated poly(methacrylic acid) brushes as effective carriers for the endothelial cells.
abstract: Endothelial cell-matrix interactions play a vital role in promoting vascularization of engineered tissues. The current study reports a facile and controllable method to develop a RGD peptide-functionalized chitosan microsphere scaffolds for rapid cell expansion of human umbilical vein endothelial cells (HUVECs). Functional poly(methacrylic acid) (PMAA) brushes are grafted from the chitosan microsphere surfaces via surface-initiated ATRP. Subsequent conjugation of RGD peptides on the pendent carboxyl groups of PMAA side chain is accomplished by carbodiimide chemistry to facilitate biocompatibility of the 3D CS scaffolding system. In vitro cell-loading assay of HUVECs exhibits a significant improvment of cell adhesion, spreading, and proliferation on the RGD peptide-immobilized CS microsphere surfaces.
authors.affiliation: Multiphase Mass Transfer & Reaction Engineering Lab, College of Chemical Engineering, Sichuan University, Chengdu, China, 610065.
authors.forename: Zhenyi
authors.initials: Z
authors.lastname: Yang
authors.forename: Shaojun
authors.initials: S
authors.lastname: Yuan
authors.forename: Bin
authors.initials: B
authors.lastname: Liang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Cleo
authors.initials: C
authors.lastname: Choong
authors.forename: Simo O
authors.initials: SO
authors.lastname: Pehkonen
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D008863:Microspheres; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000494:pharmacology
chemical_list: D004337:Drug Carriers; D009842:Oligopeptides; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; C047981:arginyl-glycyl-aspartic acid; D048271:Chitosan
keywords: ATRP; RGD peptide; chitosan microsphere; human umbilical vein endothelial cells (HUVECs); microcarriers