title: Measurement of anterior scleral curvature using anterior segment OCT.
abstract: OBJECTIVE
  To investigate and validate methods for measuring the radius of anterior scleral curvature using anterior segment optical coherence tomography images.
  
  
  METHODS
  Twenty-four volunteers were enrolled in this study. Anterior segment optical coherence tomography images, centered on horizontal/vertical limbus, including adjacent anterior sclera, were obtained in addition to conventional images centered on the optical axis. Central horizontal, nasal, and temporal optical coherence tomography images were consolidated to a new image for subsequent analyses. The reference points of limbal surface and three scleral points were marked nasally and temporally. The radius of a best-fit circle to the six scleral points was derived (the BFC [best-fit circle] method) and the radii of two circles, the centers of which are on the optical axis and pass through the points of the scleral surface at 2 mm from the limbus nasally and temporally, were calculated (the axial method). To assess the reliability and accuracy of each method, intraobserver and interobserver agreements were analyzed and the radii of contact lenses with known curvatures were measured.
  
  
  RESULTS
  The mean (±SD) radius of a BFC was 13.12 (±0.80) mm. The mean (±SD) radius of nasal anterior scleral curvature (13.33 ± 1.12 mm) was significantly greater than that of temporal anterior scleral curvature (12.32 ± 0.77 mm) (paired samples t test, p < 0.001). The BFC and axial methods showed excellent intraobserver and interobserver agreements for measurements (intraclass correlation coefficient > 0.75, p < 0.001), whereas both methods showed a tendency to slightly underestimate the actual curvature of a rigid contact lens of known dimensions (-0.07 ± 0.13 mm [the BFC method] and -0.19 ± 0.07 mm [the axial method], Wilcoxon signed rank test, p = 0.173 and p = 0.028, respectively).
  
  
  CONCLUSIONS
  Anterior segment optical coherence tomography is a valuable tool for measuring the radii of anterior scleral curvatures by image processing and mathematical calculation and can provide useful information in specific clinical situations such as designing scleral lenses.
authors.affiliation: *MD, PhD †MD Department of Ophthalmology, Seoul National University Hospital Healthcare System Gangnam Center, Seoul, Republic of Korea (HJC); Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea (HJC, S-ML, MKK, WRW); Department of Ophthalmology, Incheon Medical Center, Incheon, Republic of Korea (JYL); and Department of Ophthalmology, Eulji University Hospital, Daejeon, Republic of Korea (SYL).
authors.forename: Hyuk Jin
authors.initials: HJ
authors.lastname: Choi
authors.forename: Sang-Mok
authors.initials: SM
authors.lastname: Lee
authors.forename: Ja Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Seung Yong
authors.initials: SY
authors.lastname: Lee
authors.forename: Mee Kum
authors.initials: MK
authors.lastname: Kim
authors.forename: Won Ryang
authors.initials: WR
authors.lastname: Wee
mesh_terms: D000328:Adult; D000869:Anterior Eye Segment / Q000033:anatomy & histology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015588:Observer Variation; D009929:Organ Size; D015203:Reproducibility of Results; D012590:Sclera / Q000033:anatomy & histology*; D041623:Tomography, Optical Coherence / Q000379:methods*; D055815:Young Adult