title: Etiopathogenesis of Sheehan's Syndrome: Roles of Coagulation Factors and TNF-Alpha.
abstract: Sheehan's Syndrome (SS) is defined as pituitary hormone deficiency due to ischemic infarction of the pituitary gland as a result of massive postpartum uterine hemorrhage. Herein, we aimed to investigate the roles of Factor II (G20210A), Factor V (G1691A), MTHFR (C677T and A1298C), PAI-1 4G/5G, and TNF- α (-308  G > A) gene polymorphisms in the etiopathogenesis of SS. Venous blood samples were obtained from 53 cases with SS and 43 healthy women. Standard methods were used to extract the genomic DNAs. Factor II (G20210A), Factor V (G1691A), and MTHFR (C677T and A1298C) polymorphisms were identified by real-time PCR. PAI-1 4G/5G and TNF- α (-308  G > A) gene polymorphisms were detected with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. According to statistical analysis, none of the polymorphisms were found to be significantly higher in the SS group compared to the control group. Hence, we suggest that genetic factors other than Factor II, Factor V, MTHFR, PAI-1, and TNF- α gene polymorphisms should be researched in the etiopathogenesis of SS.
authors.affiliation: Department of Endocrinology, Erciyes University Medical School, 38039 Kayseri, Turkey.
authors.forename: Halit
authors.identifier: 0000-0003-2519-5978
authors.initials: H
authors.lastname: Diri
authors.affiliation: Department of Medical Biology, Erciyes University Medical School, 38039 Kayseri, Turkey.
authors.forename: Elif Funda
authors.identifier: 0000-0002-5644-5442
authors.initials: EF
authors.lastname: Sener
authors.affiliation: Department of Endocrinology, Erciyes University Medical School, 38039 Kayseri, Turkey.
authors.forename: Fahri
authors.initials: F
authors.lastname: Bayram
authors.affiliation: Department of Medical Genetics, Erciyes University Medical School, 38039 Kayseri, Turkey.
authors.forename: Nazife
authors.initials: N
authors.lastname: Tascioglu
authors.affiliation: Department of Endocrinology, Erciyes University Medical School, 38039 Kayseri, Turkey.
authors.forename: Yasin
authors.initials: Y
authors.lastname: Simsek
authors.affiliation: Department of Medical Genetics, Erciyes University Medical School, 38039 Kayseri, Turkey.
authors.forename: Munis
authors.initials: M
authors.lastname: Dundar