title: Antineutrophil cytoplasm antibody: positivity and clinical correlation.
abstract: OBJECTIVE
  To determine positivity and clinical correlation of anti-neutrophil cytoplasmic antibodies (ANCA), taking into account the interference of antinuclear antibodies (ANA).
  
  
  METHODS
  A prospective study was conducted in the Laboratory of Immunology of the National Cuban Center of Medical Genetic during one year. Two hounded sixty-seven patients with indication for ANCA determination were included. ANCA and ANA determinations with different cut off points and assays were determined by indirect immunofluorescense. Anti proteinase 3 and antimyeloperoxidase antibodies were determined by ELISA.
  
  
  RESULTS
  Most positivity for ANCA was seen in patients with ANCA associated, primary small-vessel vasculitides, rheumatoid arthritis and systemic lupus erythematosus. Presence of ANCA without positivity for proteinase 3 and myeloperoxidase was higher in patients with ANA and little relation was observed between the perinuclear pattern confirmed in formalin and specificity by myeloperoxidase. Highest sensibility and specificity values for vasculitides diagnostic were achieved by ANCA determination using indirect immunofluorescense with a cut off 1/80 and confirming antigenic specificities with ELISA.
  
  
  CONCLUSIONS
  ANCA can be present in a great number of chronic inflammatory or autoimmune disorders in the population studied. This determination using indirect immunofluorescence and following by ELISA had a great value for vasculitis diagnosis. Anti mieloperoxidasa assay has a higher utility than the formalin assay when ANA is present.
authors.affiliation: Laboratorio de Inmunología, Centro Nacional de Genética Médica, La Habana, Cuba. Electronic address: goity@infomed.sld.cu.
authors.forename: Goitybell
authors.initials: G
authors.lastname: Martínez Téllez
authors.affiliation: Laboratorio de Inmunología, Centro Nacional de Genética Médica, La Habana, Cuba.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Torres Rives
authors.affiliation: Laboratorio de Inmunología, Centro Nacional de Genética Médica, La Habana, Cuba.
authors.forename: Suchiquil
authors.initials: S
authors.lastname: Rangel Velázquez
authors.affiliation: Laboratorio de Inmunología, Centro Nacional de Genética Médica, La Habana, Cuba.
authors.forename: Vicky
authors.initials: V
authors.lastname: Sánchez Rodríguez
authors.affiliation: Laboratorio de Inmunología, Centro Nacional de Genética Médica, La Habana, Cuba.
authors.forename: María Antonia
authors.initials: MA
authors.lastname: Ramos Ríos
authors.affiliation: Centro Nacional de Genética Médica, La Habana, Cuba.
authors.forename: Lisset Evelyn
authors.initials: LE
authors.lastname: Fuentes Smith
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D000974:Antibodies, Antinuclear / Q000097:blood; D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000276:immunology; D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007223:Infant; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D053146:Myeloblastin / Q000276:immunology; D009195:Peroxidase / Q000276:immunology; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014657:Vasculitis / Q000175:diagnosis / Q000276:immunology; D055815:Young Adult
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D000974:Antibodies, Antinuclear; D015415:Biomarkers; D009195:Peroxidase; D053146:Myeloblastin
keywords: Anticuerpos antinucleares; Anticuerpos contra el citoplasma del neutrófilo; Antineutophil cytoplasmic antibodies; Antinuclear antibodies; Diagnostic value; Mieloperoxidasa; Myeloperoxidase; Proteinasa 3; Proteinase 3; Valor diagnóstico; Vasculitis