title: From nucleobases to nucleolipids: an ITC approach on the thermodynamics of their interactions in aqueous solutions.
abstract: Hybrid constructions based on nucleosides and lipophilic components, known as nucleolipids, have become an extremely interesting class of molecules, especially for their potential biomedical applications. In this matter, it seemed important to define the nature and estimate the strength of their interaction with polynucleotides by different ways. We report in this work a systematic investigation through isothermal titration calorimetry of the thermodynamics of the association and dissociation of adenine and thymine derivatives, not previously performed. Then we use the results obtained on these simple systems as a basis for comparison with the binding of phospholipids functionalized with adenosine and thymidine to polyadenylic or polyuridylic acids applying the same experimental technique.
authors.affiliation: Centre de Recherche Paul Pascal, 33600 Pessac, France.
authors.forename: Angelos
authors.initials: A
authors.lastname: Thanassoulas
authors.forename: Philippe
authors.initials: P
authors.lastname: Barthélémy
authors.forename: Laurence
authors.initials: L
authors.lastname: Navailles
authors.forename: Gilles
authors.initials: G
authors.lastname: Sigaud
mesh_terms: D000225:Adenine / Q000737:chemistry*; D002151:Calorimetry; D011061:Poly A / Q000737:chemistry; D011072:Poly U / Q000737:chemistry; D012995:Solubility; D013696:Temperature; D013816:Thermodynamics; D013941:Thymine / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D011061:Poly A; D011072:Poly U; D000225:Adenine; D013941:Thymine