title: Salivary antimicrobial proteins associate with age-related changes in streptococcal composition in dental plaque.
abstract: Secretion of antimicrobial proteins (AMPs) and salivary antibodies can modify biofilm formation at host body surfaces. In adolescents, associations have been reported between dental caries and salivary AMPs. AMPs demonstrate direct antimicrobial effects at high concentrations, and at lower more physiological concentrations they mediate changes in host cell defenses, which may alter the local environment and indirectly shape local biofilm formation. The expression of salivary AMPs in preschool children, at an age when the oral bacteria are known to change, has not been investigated. We sought to investigate salivary AMP expression in the context of previously well-documented changes in the oral cavities of this age group including salivary immunoglobulin A (IgA), oral bacteria and dental caries. Dental plaque and saliva were collected from 57 children aged 12-24 months at baseline, of whom 23 children were followed-up at 3 years of age. At each time, saliva was assessed for LL37, human neutrophil peptides 1-3, calprotectin, lactoferrin, salivary IgA, total plaque bacteria and Streptococcus mutans. Over time, concentrations of AMPs, S. mutans and bacteria-specific salivary IgA increased. Caries experience was also recorded when children were 3 years old. Concentrations of AMPs were highest in the saliva of 3-year-old children with the greatest burden of S. mutans. These data suggest that salivary AMPs are variable over time and between individuals, and are linked with bacterial colonization. At follow up, the majority of children remained caries free. Larger longitudinal studies are required to confirm whether salivary AMP levels are predictive of caries and whether their modulation offers therapeutic benefit.
authors.affiliation: Infection and Immunity Research Group, Glasgow Dental School, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
authors.forename: J
authors.initials: J
authors.lastname: Malcolm
authors.forename: A
authors.initials: A
authors.lastname: Sherriff
authors.forename: D F
authors.initials: DF
authors.lastname: Lappin
authors.forename: G
authors.initials: G
authors.lastname: Ramage
authors.forename: D I
authors.initials: DI
authors.lastname: Conway
authors.forename: L M D
authors.initials: LM
authors.lastname: Macpherson
authors.forename: S
authors.initials: S
authors.lastname: Culshaw
mesh_terms: D023181:Antimicrobial Cationic Peptides; D058491:Bacterial Load; D018441:Biofilms; D054804:Cathelicidins / Q000032:analysis; D002675:Child, Preschool; D003731:Dental Caries; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007223:Infant; D007781:Lactoferrin / Q000032:analysis; D039841:Leukocyte L1 Antigen Complex / Q000032:analysis; D008297:Male; D009055:Mouth / Q000382:microbiology; D012463:Saliva / Q000737:chemistry* / Q000276:immunology*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D013291:Streptococcus / Q000254:growth & development* / Q000276:immunology* / Q000502:physiology; D013295:Streptococcus mutans / Q000254:growth & development / Q000276:immunology / Q000502:physiology; D023084:alpha-Defensins / Q000032:analysis
chemical_list: D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins; D007071:Immunoglobulin A, Secretory; D039841:Leukocyte L1 Antigen Complex; D012471:Salivary Proteins and Peptides; D023084:alpha-Defensins; C406416:human neutrophil peptide 2; C406427:human neutrophil peptide 3; D007781:Lactoferrin
keywords: Streptococcus; antimicrobial proteins; innate immunity