title: Ubiquitination-mediated interaction among domains is responsible for inhibition of RNA endonuclease activity of mRNA cycling sequence binding protein from L. donovani (LdCSBP).
abstract: In nearly complete absence of transcriptional regulation, messenger RNA (mRNA) turnover mediated through specific cis-elements plays a predominant role in the control of differential gene expression for the disease causing trypanosomatid parasites. In these organisms, the periodic accumulation of S-phase messages during cell cycle is determined by the presence of one or more copies of a conserved CAUAGAAG octanucleotide motif in the untranslated regions of mRNAs. In our previous studies, a multi-domain cycling sequence binding protein LdCSBP from Leishmania donovani was characterized, which binds specifically to the octamer-containing RNAs via its uniquely arranged CCCH-type Zn fingers and degrades them through its small MutS-related (Smr) endonuclease domain, indicative of its potential role in the turnover of the S-phase mRNAs. Interestingly, the protein is modified by the incorporation of a monoubiquitin residue, and the posttranslational modification inhibits its riboendonuclease activity. However, the mechanism of such inhibition was previously unknown. Here, we establish that the CCCH-type Zn finger domain is the site of ubiquitination in LdCSBP and the interaction of CUE domain of the protein with the ubiquitinated Zn finger domain is responsible for inhibition of its riboendonuclease activity. The findings elucidate an inhibitory mechanism of RNA cleavage through ubiquitination-mediated intramolecular interaction among domains of the enzyme. Furthermore, the riboendonuclease activity is inhibited by anti-leishmanial drug paromomycin suggesting that the regulation of RNA metabolism could be a target of the drug.
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, 700064, India.
authors.forename: Kasturi
authors.initials: K
authors.lastname: Guha
authors.forename: Dipankar
authors.initials: D
authors.lastname: Bhandari
authors.forename: Titash
authors.initials: T
authors.lastname: Sen
authors.forename: Partha
authors.initials: P
authors.lastname: Saha
mesh_terms: D000818:Animals; D004722:Endoribonucleases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000737:chemistry; D005786:Gene Expression Regulation; D007893:Leishmania donovani / Q000201:enzymology / Q000235:genetics*; D010303:Paromomycin / Q000737:chemistry; D054730:Protein Interaction Domains and Motifs; D015800:Protozoan Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D061987:Sf9 Cells; D054875:Ubiquitination; D016335:Zinc Fingers
chemical_list: D004791:Enzyme Inhibitors; D015800:Protozoan Proteins; D016601:RNA-Binding Proteins; D010303:Paromomycin; D012313:RNA; D004722:Endoribonucleases