title: Dissemination in Japan of multidrug-resistant Pseudomonas aeruginosa isolates producing IMP-type metallo-β-lactamases and AAC(6')-Iae/AAC(6')-Ib.
abstract: The spread throughout Japan of antibiotic-resistance factors in multidrug-resistant (MDR) Pseudomonas aeruginosa isolates was investigated epidemiologically, using immunochromatographic assays specific for IMP-type metallo-β-lactamases (IMPs) and aminoglycoside 6'-N-acetyltransferase [AAC(6')]-Iae and -Ib. Three hundred MDR P. aeruginosa isolates were obtained during each of two years, 2011 and 2012, from 190 hospitals in 39 prefectures in Japan. The percentage of P. aeruginosa isolates producing IMPs, AAC(6')-Iae or AAC(6')-Ib increased significantly from 170/300 (56.7%) in 2011 to 230/300 (76.7%) in 2012, with 134/170 (78.8%) in 2011 and 179/230 (77.8%) in 2012 producing both IMP and either AAC(6')-Iae or AAC(6')-Ib. The MICs of antibiotics, including cephalosporins and carbapenems, were markedly higher for isolates that did than did not produce these resistance factors. These results indicated that MDR P. aeruginosa producing IMPs, AAC(6')-Iae or AAC(6')-Ib have spread throughout Japan and that these antibiotic-resistance factors are useful markers for monitoring MDR P. aeruginosa in Japan.
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama 1-21-1, Shinjuku, Tokyo 162-8655, Japan; Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Tojo
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama 1-21-1, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tada
authors.affiliation: BML Inc., Kawagoe, Saitama, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shimojima
authors.affiliation: Mizuho Medy Co., Ltd. R&D, Tosu, Saga, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Mizuho Medy Co., Ltd. R&D, Tosu, Saga, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Narahara
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama 1-21-1, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama 1-21-1, Shinjuku, Tokyo 162-8655, Japan. Electronic address: tkirikae@ri.ncgm.go.jp.
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
authors.affiliation: Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Ohmagari
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001426:Bacterial Proteins / Q000378:metabolism; D015780:Carbapenems / Q000627:therapeutic use; D002511:Cephalosporins / Q000627:therapeutic use; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007291:Inosine Monophosphate / Q000378:metabolism*; D007564:Japan; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D002511:Cephalosporins; D007291:Inosine Monophosphate; D000123:Acetyltransferases; C056532:aminoglycoside N(6')-acetyltransferase; D001618:beta-Lactamases
keywords: AAC; IMP; Immunochromatographic assay; Multidrug-resistance; Pseudomonas aeruginosa