title: Simultaneous analysis of seven 2-hydroxy fatty acids as tert-butyldimethylsilyl derivatives in plasma by gas chromatography-mass spectrometry.
abstract: An efficient method for the simultaneous analysis of seven 2-hydroxy fatty acids (2-HFAs) as tert-butyldimethylsilyl (TBDMS) derivative was developed by gas chromatography-mass spectrometry in selected ion monitoring mode. New mass spectral data on 2-hydroxycapric, 2-hydroxypalmitic, 2-hydroxystearic and 2-hydroxybehenic acids as di-TBDMS derivatives for hydroxyl and carboxyl groups were built. Under the optimal conditions, the present method showed a good correlation coefficient (r ≥ 0.999) in the range of 0.01-0.5 µg. The precision showed low relative standard deviation of <10%, and the accuracy (percentage relative error) varied from -5.2 to 0.3 for the seven 2-HFAs studied. Recovery rates of all 2-HFAs were  ≥ 93.2% with good precision. When applied to normal human plasma, seven 2-HFAs were positively identified. Therefore, the present efficient method will be useful for simultaneous analysis of 2-HFAs in plasma.
authors.affiliation: College of Pharmacy, Sunchon National University, Suncheon, 540-950, Republic of Korea.
authors.forename: Chan
authors.initials: C
authors.lastname: Seo
authors.forename: Jaehwan
authors.initials: J
authors.lastname: Yoon
authors.forename: Yumie
authors.initials: Y
authors.lastname: Rhee
authors.forename: Jong-Jin
authors.initials: JJ
authors.lastname: Kim
authors.forename: Sang-Jip
authors.initials: SJ
authors.lastname: Nam
authors.forename: Wonjae
authors.initials: W
authors.lastname: Lee
authors.forename: Gwang
authors.initials: G
authors.lastname: Lee
authors.forename: Sung-Tae
authors.initials: ST
authors.lastname: Yee
authors.forename: Man-Jeong
authors.initials: MJ
authors.lastname: Paik
mesh_terms: D005227:Fatty Acids / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D012821:Silanes; C103256:tert-butyldimethylsilane
keywords: 2-hydroxy fatty acid; gas chromatography-mass spectrometry; plasma; tert-butyldimethylsilylation