title: Recent advances in DNA assembly technologies.
abstract: DNA assembly is one of the most important foundational technologies for synthetic biology and metabolic engineering. Since the development of the restriction digestion and ligation method in the early 1970s, a significant amount of effort has been devoted to developing better DNA assembly methods with higher efficiency, fidelity, and modularity, as well as simpler and faster protocols. This review will not only summarize the key DNA assembly methods and their recent applications, but also highlight the innovations in assembly schemes and the challenges in automating the DNA assembly methods.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaig, Urbana, IL, USA.
authors.forename: Ran
authors.initials: R
authors.lastname: Chao
authors.affiliation: Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaig, Urbana, IL, USA.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaig, Urbana, IL, USA zhao5@illinois.edu.|Departments of Chemistry, Biochemistry, and Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
mesh_terms: D004247:DNA / Q000235:genetics*; D060847:Metabolic Engineering; D058615:Synthetic Biology
chemical_list: D004247:DNA
keywords: automation; genome synthesis; metabolic engineering; pathway construction; pathway optimization; synthetic biology