title: Capsaicin-induced transcriptional changes in hypothalamus and alterations in gut microbial count in high fat diet fed mice.
abstract: Obesity is a global health problem and recently it has been seen as a growing concern for developing countries. Several bioactive dietary molecules have been associated with amelioration of obesity and associated complications and capsaicin is one among them. The present work is an attempt to understand and provide evidence for the novel mechanisms of anti-obesity activity of capsaicin in high fat diet (HFD)-fed mice. Swiss albino mice divided in three groups (n=8-10) i.e. control, HFD fed and capsaicin (2mg/kg, po)+HFD fed were administered respective treatment for 3months. After measuring phenotypic and serum related biochemical changes, effect of capsaicin on HFD-induced transcriptional changes in hypothalamus, white adipose tissue (WAT) (visceral and subcutaneous), brown adipose tissue (BAT) and gut microbial alterations was studied and quantified. Our results suggest that, in addition to its well-known effects, oral administration of capsaicin (a) modulates hypothalamic satiety associated genotype, (b) alters gut microbial composition, (c) induces "browning" genotype (BAT associated genes) in subcutaneous WAT and (d) increases expression of thermogenesis and mitochondrial biogenesis genes in BAT. The present study provides evidence for novel and interesting mechanisms to explain the anti-obesity effect of capsaicin.
authors.affiliation: National Agri-Food Biotechnology Institute, SAS Nagar, Punjab, India 160071.
authors.forename: Ritesh K
authors.initials: RK
authors.lastname: Baboota
authors.affiliation: National Agri-Food Biotechnology Institute, SAS Nagar, Punjab, India 160071.
authors.forename: Nida
authors.initials: N
authors.lastname: Murtaza
authors.affiliation: National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab, India 160062.
authors.forename: Sneha
authors.initials: S
authors.lastname: Jagtap
authors.affiliation: National Agri-Food Biotechnology Institute, SAS Nagar, Punjab, India 160071.
authors.forename: Dhirendra P
authors.initials: DP
authors.lastname: Singh
authors.affiliation: National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab, India 160062.
authors.forename: Aniket
authors.initials: A
authors.lastname: Karmase
authors.affiliation: Department of Biotechnology, University Institute of Engineering and Technology, Panjab University, Chandigarh, India 160025.
authors.forename: Jaspreet
authors.initials: J
authors.lastname: Kaur
authors.affiliation: National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab, India 160062.
authors.forename: Kamlesh K
authors.initials: KK
authors.lastname: Bhutani
authors.affiliation: Department of Biochemistry, Panjab University, Chandigarh, India 160014.
authors.forename: Ravneet K
authors.initials: RK
authors.lastname: Boparai
authors.affiliation: Department of Pharmacology, Southern Illinois University School of Medicine, Springfield, IL 62702, USA.
authors.forename: Louis S
authors.initials: LS
authors.lastname: Premkumar
authors.affiliation: National Agri-Food Biotechnology Institute, SAS Nagar, Punjab, India 160071.
authors.forename: Kanthi Kiran
authors.initials: KK
authors.lastname: Kondepudi
authors.affiliation: National Agri-Food Biotechnology Institute, SAS Nagar, Punjab, India 160071. Electronic address: mbishnoi@gmail.com.
authors.forename: Mahendra
authors.initials: M
authors.lastname: Bishnoi
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D001483:Base Sequence; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002211:Capsaicin / Q000494:pharmacology*; D015169:Colony Count, Microbial; D017931:DNA Primers; D059305:Diet, High-Fat; D005247:Feeding Behavior / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D008297:Male; D051379:Mice; D014158:Transcription, Genetic / Q000187:drug effects*; D015430:Weight Gain / Q000187:drug effects
chemical_list: D001786:Blood Glucose; D017931:DNA Primers; D002211:Capsaicin
keywords: Anti-obesity; Capsaicin; Gut microflora; Hypothalamus; TRPV1