title: Proteome-wide analysis and diel proteomic profiling of the cyanobacterium Arthrospira platensis PCC 8005.
abstract: The filamentous cyanobacterium Arthrospira platensis has a long history of use as a food supply and it has been used by the European Space Agency in the MELiSSA project, an artificial microecosystem which supports life during long-term manned space missions. This study assesses progress in the field of cyanobacterial shotgun proteomics and light/dark diurnal cycles by focusing on Arthrospira platensis. Several fractionation workflows including gel-free and gel-based protein/peptide fractionation procedures were used and combined with LC-MS/MS analysis, enabling the overall identification of 1306 proteins, which represents 21% coverage of the theoretical proteome. A total of 30 proteins were found to be significantly differentially regulated under light/dark growth transition. Interestingly, most of the proteins showing differential abundance were related to photosynthesis, the Calvin cycle and translation processes. A novel aspect and major achievement of this work is the successful improvement of the cyanobacterial proteome coverage using a 3D LC-MS/MS approach, based on an immobilized metal affinity chromatography, a suitable tool that enabled us to eliminate the most abundant protein, the allophycocyanin. We also demonstrated that cell growth follows a light/dark cycle in A. platensis. This preliminary proteomic study has highlighted new characteristics of the Arthrospira platensis proteome in terms of diurnal regulation.
authors.affiliation: Department of Proteomics and Microbiology, Interdisciplinary Mass Spectrometry Center (CISMa), University of Mons, Mons, Belgium.
authors.forename: Sabine
authors.initials: S
authors.lastname: Matallana-Surget
authors.affiliation: Department of Proteomics and Microbiology, Interdisciplinary Mass Spectrometry Center (CISMa), University of Mons, Mons, Belgium.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Derock
authors.affiliation: Department of Proteomics and Microbiology, Interdisciplinary Mass Spectrometry Center (CISMa), University of Mons, Mons, Belgium.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Leroy
authors.affiliation: Department of Proteomics and Microbiology, Interdisciplinary Mass Spectrometry Center (CISMa), University of Mons, Mons, Belgium; Unit of Microbiology, Expert Group Molecular and Cellular Biology, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium.
authors.forename: Hanène
authors.initials: H
authors.lastname: Badri
authors.affiliation: Department of Proteomics and Microbiology, Interdisciplinary Mass Spectrometry Center (CISMa), University of Mons, Mons, Belgium.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Deschoenmaeker
authors.affiliation: Department of Proteomics and Microbiology, Interdisciplinary Mass Spectrometry Center (CISMa), University of Mons, Mons, Belgium.
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002853:Chromatography, Liquid; D002940:Circadian Rhythm / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D013058:Mass Spectrometry; D017440:Photoperiod; D010788:Photosynthesis; D010798:Phycocyanin / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053545:Spirulina / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D010798:Phycocyanin; D002734:Chlorophyll