title: Poly(ε-caprolactone) nerve conduit and local delivery of vegf and fgf2 genes stimulate neuroregeneration.
abstract: We studied regeneration of rat sciatic nerve while overcoming of a 5-mm diastasis with the aid of nanostructured conduit made of biocompatible and biodegradable poly(ε-caprolactone) and filled with fibrin hydrogel matrix. Implantation of the conduit into the nerve in combination with local delivery of the expression plasmid carrying genes encoding vascular endothelial growth factor (vegf) and fibroblast growth factor 2 (fgf2) leads to an increase in number of myelinated fibers and S-100(+) cells in the peripheral nerve stump and improved recovery of the nerve function. Under conditions of direct gene therapy, an advantage of electrospun poly(ε-caprolactone) conduit with high-porosity was revealed on the basis of these criteria in comparison with biocompatible silicon conduit.
authors.affiliation: Department of Histology, Cytology, and Embryology, Kazan State Medical University, Kazan, Russia.
authors.forename: S I
authors.initials: SI
authors.lastname: Nikolaev
authors.forename: A R
authors.initials: AR
authors.lastname: Gallyamov
authors.forename: G V
authors.initials: GV
authors.lastname: Mamin
authors.forename: Yu A
authors.initials: YA
authors.lastname: Chelyshev
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D001672:Biocompatible Materials / Q000737:chemistry*; D015415:Biomarkers / Q000378:metabolism; D055664:Electrochemical Techniques; D005337:Fibrin / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis / Q000235:genetics; D005822:Genetic Vectors; D048091:Guided Tissue Regeneration / Q000379:methods*; D020100:Hydrogels; D009186:Myelin Sheath / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D010957:Plasmids / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D009418:S100 Proteins / Q000378:metabolism; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism / Q000601:surgery; D054457:Tissue Scaffolds; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D020100:Hydrogels; D011091:Polyesters; D009418:S100 Proteins; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D016222:Fibroblast Growth Factor 2; C016240:polycaprolactone; D005337:Fibrin