title: Evaluation of the protective effect of α-lipoic acid on cisplatin ototoxicity using distortion-product otoacoustic emission measurements: an experimental animal study.
abstract: The aim of our study was to determine the effectiveness of intratympanic α-lipoic acid injection as an otoprotective agent against cisplatin-induced ototoxicity in guinea pigs. Twenty-four adult male albino guinea pigs with normal hearing were divided into 4 groups. The guinea pigs received intraperitoneal cisplatin in group 1, intraperitoneal cisplatin and intratympanic α-lipoic acid in group 2, intratympanic α-lipoic acid in group 3, as well as intraperitoneal cisplatin and intratympanic saline in group 4. Distortion-product otoacoustic emission measurements were obtained for both ears at the following time points: before administration (baseline recording) and on day 3 (72 h later). In group 1 (cisplatin), significant deterioration was observed at all frequencies on day 3 (P < 0.05). In group 2 (cisplatin + α-lipoic acid), deterioration was observed at all frequencies on day 3; however, this deterioration did not reach a statistical significance (P > 0.05). In group 3 (α-lipoic acid), no significant difference was observed between baseline and day 3 (P > 0.05). In group 4 (cisplatin + saline), deterioration was observed at all frequencies on day 3; however, this deterioration did not reach a statistical significance (P > 0.05). Cisplatin-induced hearing loss in the guinea pigs may be limited to some extent by the concomitant use of α-lipoic acid. Dose-dependent changes in the possible effects of α-lipoic acid need further investigation. Future morphologic studies may contribute to expose clearly the protective effect of α-lipoic acid.
authors.affiliation: From the Department of Otorhinolaryngology, Izmir Katip Celebi University, Ataturk Training and Research Hospital, Izmir, Turkey.
authors.forename: Yilmaz
authors.initials: Y
authors.lastname: Ozkul
authors.forename: Murat
authors.initials: M
authors.lastname: Songu
authors.forename: Mehmet Sinan
authors.initials: MS
authors.lastname: Basoglu
authors.forename: Sedat
authors.initials: S
authors.lastname: Ozturkcan
authors.forename: Huseyin
authors.initials: H
authors.lastname: Katilmis
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000627:therapeutic use*; D002945:Cisplatin / Q000633:toxicity*; D003051:Cochlea / Q000187:drug effects*; D004195:Disease Models, Animal; D004432:Ear, Middle; D006168:Guinea Pigs; D034381:Hearing Loss / Q000139:chemically induced; D007267:Injections; D007274:Injections, Intraperitoneal; D008297:Male; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects; D020011:Protective Agents / Q000627:therapeutic use*; D008063:Thioctic Acid / Q000627:therapeutic use*; D013997:Time Factors; D014722:Vestibule, Labyrinth / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D020011:Protective Agents; D008063:Thioctic Acid; D002945:Cisplatin