title: Comparison of pharmacokinetic behavior of two iridoid glycosides in rat plasma after oral administration of crude Cornus officinals and its jiuzhipin by high performance liquid chromatography triple quadrupole mass spectrometry combined with multiple reactions monitoring mode.
abstract: OBJECTIVE
  The present study examined the pharmacokinetic profiles of two iridoid glycosides named morroniside and loganin in rat plasma after oral administration of crude and processed Cornus officinals.
  
  
  METHODS
  A rapid, selective and specific high-performance liquid chromatography/electrospray ionization tandem mass spectrometry with multiple reactions monitoring mode was developed to simultaneously investigate the pharmacokinetic profiles of morroniside and loganin in rat plasma after oral administration of crude C. officinals and its jiuzhipin.
  
  
  RESULTS
  The morroniside and loganin in crude and processed C. officinals could be simultaneously determined within 7.4 min. Linear calibration curves were obtained over the concentration ranges of 45.45-4800 ng/mL for all the analytes. The intra-and inter-day precisions relative standard deviation was lesser than 2.84% and 4.12%, respectively.
  
  
  CONCLUSIONS
  The pharmacokinetic parameters of two iridoid glucosides were also compared systematically between crude and processed C. officinals. This paper provides the theoretical proofs for further explaining the processing mechanism of Traditional Chinese Medicines.
authors.affiliation: The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, P. R. China.
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
authors.affiliation: The First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Jiang
keywords: High performance liquid chromatography-triple quadrupole mass spectrometry; morroniside and loganin; multiple reactions monitoring mode; pharmacokinetics; rat plasma