title: Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials.
abstract: OBJECTIVE
  The development of technology for preparing chitosan-protein scaffolds loaded with lysostaphin, which potentially could be used as dressing for wound treatment and soft tissue infections caused by Staphylococcus aureus.
  
  
  RESULTS
  The unique technology of chitosan solubilization using gaseous CO(2) instead of organic or inorganic acids was used for the incorporation of lysostaphin, the enzyme that exhibits bactericidal activity against staphylococci, within the structure of chitosan-protein sponges. The developed chitosan-protein scaffolds loaded with lysostaphin revealed high antistaphylococcal activity, which has been confirmed with a large (n = 143) collection of clinical (skin and wound infections) and animal (bovine mastitis) isolates of these bacteria, including MRSA. No change of bactericidal activity of the lyophilized materials has been observed during half-year storage at 4°C.
  
  
  CONCLUSIONS
  The developed materials are potential candidates for preparing biologically active, antistaphylococcal wound dressing materials.
  
  
  CONCLUSIONS
  Staphylococci belong to the most popular and most burdensome aetiological factors of wound and soft tissues infections. The developed chitosan-protein scaffolds loaded with lysostaphin could be a possible solution to problems associated with treatment of these infections.
authors.affiliation: Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Gdańsk, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Szweda
authors.forename: G
authors.initials: G
authors.lastname: Gorczyca
authors.forename: R
authors.initials: R
authors.lastname: Tylingo
authors.forename: J
authors.initials: J
authors.lastname: Kurlenda
authors.forename: J
authors.initials: J
authors.lastname: Kwiecinski
authors.forename: S
authors.initials: S
authors.lastname: Milewski
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology*; D001458:Bandages; D002417:Cattle; D048271:Chitosan; D005260:Female; D008248:Lysostaphin / Q000008:administration & dosage / Q000494:pharmacology*; D008414:Mastitis, Bovine / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology; D013207:Staphylococcal Skin Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects*; D014946:Wound Infection / Q000382:microbiology
chemical_list: D000891:Anti-Infective Agents, Local; D048271:Chitosan; D008248:Lysostaphin
keywords: carbon dioxide; chitosan; collagen; lysostaphin; wound dressing