title: Isolation of Moraxella bovoculi from racehorses with keratoconjunctivitis.
abstract: Moraxella bovoculi was isolated and identified in ocular fluid samples collected from 9 racehorses with infectious keratoconjunctivitis in China in 2013. All 9 M. bovoculi isolates were hemolytic, Gram-negative diplococci that were phenylalanine deaminase positive. The sequence of the 16S ribosomal DNA (rDNA) gene of the isolates matched the 16S rDNA sequence of M. bovoculi. Amplification of the 16S-23S intergenic spacer region followed by AfaI digestion produced a 600-base pair product, a result characteristic of M. bovoculi isolates. The phylogenetic analysis based on the 16S rDNA sequence confirmed the strain isolated in the current study had genetic homology with M. bovoculi.
authors.affiliation: National Research Center for Wildlife-borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing (Liu, J Yan, Q Yan, He), P.R. China.|University of Chinese Academy of Sciences, Beijing (Liu), P.R. China.|General Station of Animal Husbandry of Beijing (Wang), P.R. China.|Henan Center for Animal Disease Control & Prevention, Animal Husbandry Bureau of Henan Province, Zhengzhou (R Yan), P.R. China.|Zhengzhou Zhongdao Biotechnology Co. Ltd. (Zhao), P.R. China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Liu
authors.affiliation: National Research Center for Wildlife-borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing (Liu, J Yan, Q Yan, He), P.R. China.|University of Chinese Academy of Sciences, Beijing (Liu), P.R. China.|General Station of Animal Husbandry of Beijing (Wang), P.R. China.|Henan Center for Animal Disease Control & Prevention, Animal Husbandry Bureau of Henan Province, Zhengzhou (R Yan), P.R. China.|Zhengzhou Zhongdao Biotechnology Co. Ltd. (Zhao), P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yan
authors.affiliation: National Research Center for Wildlife-borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing (Liu, J Yan, Q Yan, He), P.R. China.|University of Chinese Academy of Sciences, Beijing (Liu), P.R. China.|General Station of Animal Husbandry of Beijing (Wang), P.R. China.|Henan Center for Animal Disease Control & Prevention, Animal Husbandry Bureau of Henan Province, Zhengzhou (R Yan), P.R. China.|Zhengzhou Zhongdao Biotechnology Co. Ltd. (Zhao), P.R. China.
authors.forename: Yutian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Research Center for Wildlife-borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing (Liu, J Yan, Q Yan, He), P.R. China.|University of Chinese Academy of Sciences, Beijing (Liu), P.R. China.|General Station of Animal Husbandry of Beijing (Wang), P.R. China.|Henan Center for Animal Disease Control & Prevention, Animal Husbandry Bureau of Henan Province, Zhengzhou (R Yan), P.R. China.|Zhengzhou Zhongdao Biotechnology Co. Ltd. (Zhao), P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yan
authors.affiliation: National Research Center for Wildlife-borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing (Liu, J Yan, Q Yan, He), P.R. China.|University of Chinese Academy of Sciences, Beijing (Liu), P.R. China.|General Station of Animal Husbandry of Beijing (Wang), P.R. China.|Henan Center for Animal Disease Control & Prevention, Animal Husbandry Bureau of Henan Province, Zhengzhou (R Yan), P.R. China.|Zhengzhou Zhongdao Biotechnology Co. Ltd. (Zhao), P.R. China.
authors.forename: Linping
authors.initials: L
authors.lastname: Zhao
authors.affiliation: National Research Center for Wildlife-borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing (Liu, J Yan, Q Yan, He), P.R. China.|University of Chinese Academy of Sciences, Beijing (Liu), P.R. China.|General Station of Animal Husbandry of Beijing (Wang), P.R. China.|Henan Center for Animal Disease Control & Prevention, Animal Husbandry Bureau of Henan Province, Zhengzhou (R Yan), P.R. China.|Zhengzhou Zhongdao Biotechnology Co. Ltd. (Zhao), P.R. China.
authors.forename: Ruoqian
authors.initials: R
authors.lastname: Yan
authors.affiliation: National Research Center for Wildlife-borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing (Liu, J Yan, Q Yan, He), P.R. China.|University of Chinese Academy of Sciences, Beijing (Liu), P.R. China.|General Station of Animal Husbandry of Beijing (Wang), P.R. China.|Henan Center for Animal Disease Control & Prevention, Animal Husbandry Bureau of Henan Province, Zhengzhou (R Yan), P.R. China.|Zhengzhou Zhongdao Biotechnology Co. Ltd. (Zhao), P.R. China.
authors.forename: Hongxuan
authors.initials: H
authors.lastname: He
mesh_terms: D000818:Animals; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D006734:Horse Diseases / Q000175:diagnosis* / Q000382:microbiology; D006736:Horses; D007639:Keratoconjunctivitis, Infectious / Q000175:diagnosis* / Q000382:microbiology; D009016:Moraxella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D045828:Moraxellaceae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Keratoconjunctivitis; Moraxella bovoculi; racehorses