title: CD86 +1057G/A polymorphism and risk of chronic immune thrombocytopenia.
abstract: The G to A transition at position +1057 single nucleotide polymorphism site in CD86 gene results in the alanine to threonine substitution, which further affects the antigen-presenting cells' signal transduction. Thus, the purpose of this study was to determine the association between CD86 +1057G/A polymorphism and the risk for chronic immune thrombocytopenia (cITP). The CD86 +1057G/A polymorphism in 158 cITP patients and 150 healthy controls were detected by polymerase chain reaction restriction fragment length polymorphism and then confirmed by DNA sequencing. In the patients with cITP, the frequencies of GG, AG and AA genotypes and G and A alleles were 18.4%, 58.8%, 22.8%, 47.8% and 52.2%, respectively. No difference in genotype and allele frequencies was detected in total cITP patients and normal controls (p = 0.913 and 0.845, respectively). Cases were subsequently classified by age at diagnosis, gender or clinical responses to glucocorticoids, and still no obvious discrepancy of genotype and allele frequencies was found between each of the groups and normal controls. In conclusion, this study suggests that CD86 +1057G/A polymorphism may be not associated with the genetic susceptibility to cITP in a Chinese population.
authors.affiliation: Department of Hematology, The No. 2 People's Hospital , Wuxi , People's Republic of China.
authors.forename: Pin
authors.initials: P
authors.lastname: Wu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.forename: Shiyun
authors.initials: S
authors.lastname: Lu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D051940:B7-2 Antigen / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D002681:China; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics*; D012737:Sex Factors; D055815:Young Adult
chemical_list: D051940:B7-2 Antigen; C497964:CD86 protein, human
keywords: CD86; glucocorticoids; primary immune thrombocytopenia; rs1129055; single nucleotide polymorphism