title: 23,24-Dihydrocucurbitacin C: a new compound regarded as the next metabolite of cucurbitacin C.
abstract: Cucurbitacin C, a bitter substance in Cucumis sativus L., was isolated from green leaves by using phytochemical methods. An analytical method using high-performance liquid chromatography (HPLC) was established for the quantification of cucurbitacin C in different parts of the cucumber plant at different growth periods. Cucurbitacin C was detected in the leaves and stems but not in the female flowers, fruits, roots and leafstalks. The level of cucurbitacin C decreased significantly with the process of young leaves turning old. A new compound named 23,24-dihydrocucurbitacin C, regarded as the next metabolite of cucurbitacin C, was determined unambiguously by HPLC-quadrupole-time-of-flight mass spectrometry and nuclear magnetic resonance.
authors.affiliation: a School of Pharmacy, Hunan University of Chinese Medicine , Changsha 410208 , P.R. China.
authors.forename: Zhi-Xing
authors.initials: ZX
authors.lastname: Qing
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiu-Bin
authors.initials: XB
authors.lastname: Liu
authors.forename: Pi
authors.initials: P
authors.lastname: Cheng
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zeng
mesh_terms: D002851:Chromatography, High Pressure Liquid; D018553:Cucumis sativus / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology
chemical_list: C000592993:23,24-dihydrocucurbitacin C; D014315:Triterpenes; C474429:cucurbitacin C
keywords: 23,24-dihydrocucurbitacin C; Cucumis sativus L.; HPLC-Q-TOF-MS; NMR; cucurbitacin C