title: Morphological changes in the liver and kidneys of rats subjected to terminal ileum exclusion during obstructive cholestasis.
abstract: OBJECTIVE
  To investigate the effects of ileal exclusion on hepatic and renal morphology in extra-hepatic cholestasis.
  
  
  METHODS
  Twenty four rats were distributed into three groups. Group 1 (control) underwent laparotomy and laparorrhaphy. The animals in groups 2 and 3 underwent hepatic duct ligature and kept in cholestasis for four weeks. After this period, the rats in groups 2 and 3 underwent internal biliary derivation. In Group 3, the last ten centimeters of the terminal ileum were by passed and excluded. Four weeks later, histological and biochemical analysis were performed in all animals of the three groups.
  
  
  RESULTS
  In Group 1, no abnormalities regarding hepatic morphology were observed. All animals from groups 2 and 3 presented hepatic fibrosis. No difference was observed between the two groups. No morphological differences in renal histology could be identified among the three groups. There were differences in AST (p<0.05), ALT (p<0.05), direct bilirubin (p<0.05), ƔGT (p<0.05), urea (p<0.05) and creatinine (p<0.05) in Group 3 compared to control.
  
  
  CONCLUSIONS
  The distal ileum exclusion had no influence upon the hepatic and renal morphological alterations, and biochemical liver and kidney tests have worsened.
authors.affiliation: Department of Surgery, Faculty of Medicine, Minas Gerais Federal University, Belo Horizonte, MG, Brazil.
authors.forename: Evandro Luis de Oliveira
authors.initials: EL
authors.lastname: Costa
authors.affiliation: Department of Morphology, Biological Sciences Institute, UFMG, Belo Horizonte, MG, Brazil.
authors.forename: Geraldo Magela de
authors.initials: GM
authors.lastname: Azevedo
authors.affiliation: Department of Surgery, Faculty of Medicine, UFMG, Belo Horizonte, MG, Brazil.
authors.forename: Andy
authors.initials: A
authors.lastname: Petroianu
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001651:Cholestasis, Extrahepatic / Q000473:pathology / Q000601:surgery*; D005355:Fibrosis; D007082:Ileum / Q000473:pathology / Q000601:surgery*; D007668:Kidney / Q000473:pathology*; D007813:Laparotomy / Q000379:methods; D008026:Ligation; D008099:Liver / Q000473:pathology*; D008103:Liver Cirrhosis; D008297:Male; D011537:Pruritus / Q000517:prevention & control; D017208:Rats, Wistar; D015203:Reproducibility of Results; D016896:Treatment Outcome