title: Gene-gene interaction of ACE I/D, endothelial nitric oxide synthase 4 a/b and ApoE does not affect coronary artery disease severity.
abstract: OBJECTIVE
  Previous studies have shown the impact of angiotensin converting enzyme (ACE) insertion/deletion (I/D), endothelial nitric oxide synthase (eNOS) polymorphisms and ApoE genotypes on coronary artery disease (CAD). The aim of this study is to investigate the relationship between the genetic polymorphisms and the severity of CAD and to evaluate their potential interactions.
  
  
  METHODS
  All patients underwent coronary angiography; coronary score (CS) and severity score (SS) were calculated for them. ACE I/D, eNOS and ApoE polymorphisms were detected by polymerase chain reaction (PCR).
  
  
  RESULTS
  Neither CS nor SS showed a direct relationship with eNOS and ApoE genotypes. CS and SS were found to be high in patients carrying the ACE DD allele (p = 0.034 and p = 0.009). In the gene interactions, there was an increase in the SS only in patients with coexisting eNOS b/b genotype and ACE DD allele (p = 0.043).
  
  
  CONCLUSIONS
  The interactions of the gene polymorphisms investigated don't play an important role in determining an individual's risk for the severity of CAD.
authors.affiliation: Department of Biochemistry, Çukurova University, Adana, Turkey.
authors.forename: Selçuk
authors.initials: S
authors.lastname: Matyar
authors.affiliation: Department of Cardiology, Çukurova University, Adana, Turkey.
authors.forename: Esmeray
authors.initials: E
authors.lastname: Acartürk
authors.affiliation: Department of Biochemistry, Çukurova University, Adana, Turkey.
authors.forename: Gülen
authors.initials: G
authors.lastname: Attila
authors.affiliation: Department of Biostatistics, Çukurova University, Adana, Turkey.
authors.forename: Ilker
authors.initials: I
authors.lastname: Ünal
authors.affiliation: Department of Cardiology, Çukurova University, Adana, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Soyer
authors.affiliation: Department of Cardiology, Çukurova University, Adana, Turkey.
authors.forename: Onur
authors.initials: O
authors.lastname: Akpınar
mesh_terms: D000328:Adult; D000368:Aged; D001057:Apolipoproteins E / Q000235:genetics*; D003324:Coronary Artery Disease / Q000235:genetics*; D004843:Epistasis, Genetic; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012720:Severity of Illness Index
chemical_list: D001057:Apolipoproteins E; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D007703:Peptidyl-Dipeptidase A