title: Expression, purification and preliminary crystallographic analysis of Mycobacterium tuberculosis CysQ, a phosphatase involved in sulfur metabolism.
abstract: CysQ is part of the sulfur-activation pathway that dephosphorylates 3'-phosphoadenosine 5'-monophosphate (PAP) to regenerate adenosine 5'-monophosphate (AMP) and free phosphate. PAP is the product of sulfate-transfer reactions from sulfotransferases that use the universal sulfate donor 3'-phosphoadenosine 5'-phosphosulfate (PAPS). In some organisms PAP is also the product of PAPS reductases that reduce sulfate from PAPS to sulfite. CysQ from Mycobacterium tuberculosis, which plays an important role in the biosynthesis of sulfated glycoconjugates, was successfully purified and crystallized in 24% PEG 1500, 20% glycerol. X-ray diffraction data were collected to 1.7 Å resolution using a synchrotron-radiation source. Crystals grew in the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a=40.3, b=57.9, c=101.7 Å and with one monomer per asymmetric unit.
authors.affiliation: Graduate Program in Biochemistry, Molecular, Cellular and Developmental Biology, University of California, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Anna I
authors.initials: AI
authors.lastname: Erickson
authors.affiliation: Department of Molecular and Cellular Biology, University of California, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Reta D
authors.initials: RD
authors.lastname: Sarsam
authors.affiliation: Graduate Program in Biochemistry, Molecular, Cellular and Developmental Biology, University of California, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Fisher
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D018360:Crystallography, X-Ray; D017931:DNA Primers; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D013455:Sulfur; D010744:Phosphoric Monoester Hydrolases
keywords: 3′,5′-bisphosphate nucleotidase; 3′-phosphoadenosine 5′-phosphate; CysQ; Mycobacterium tuberculosis; PAP; Rv2131c; monophosphatase