title: [Down-regulation of CD59 inhibits proliferation and promotes apoptosis of HeLa cells].
abstract: OBJECTIVE
  To intervene the expression of CD59 on cervical cancer HeLa cells by RNA interference and phage display random peptide library, respectively, and detect the effect of down-regulated CD59 expression on proliferation and apoptosis of the cervical cancer cells.
  
  
  METHODS
  HeLa cells were divided into normal control group, CD59 peptide seal group, pSUPER transfected group and pSUPER-siCD59 transfected group. The CD59 peptide seal group was treated with 10 μg/mL CD59 peptide seal for 8 hours. The pSUPER and pSUPER-siCD59 transfected groups were transfected with empty plasmid pSUPER and recombinant plasmid pSUPER-siCD59, respectively. MTT assay was used for the detection of cell proliferation. TUNEL, annexin V-PE/7-AAD staining combined with flow cytometry were adopted for the detection of cell apoptosis.
  
  
  RESULTS
  Compared with the control groups, both CD59 peptide seal group and pSUPER-siCD59 transfected group presented a diminished cell proliferation activity and a strengthened apoptosis. What's more, the effect of peptide seal on CD59 expression inhibition was better than that of pSUPER-siCD59 plasmid.
  
  
  CONCLUSIONS
  Down-regulation of CD59 expression could inhibit HeLa cells' proliferation and promote apoptosis, and the inhibitory effect of peptide seal was better than that of CD59 interference plasmid.
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D018957:CD59 Antigens / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D015536:Down-Regulation; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D020287:In Situ Nick-End Labeling; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology; D034622:RNA Interference
chemical_list: D018957:CD59 Antigens; D010455:Peptides