title: PARP inhibitor activates the intrinsic pathway of apoptosis in primary lung cancer cells.
abstract: This investigation was aimed to see whether PJ34(TM), a PARP inhibitor, could exert cytotoxicity in six nonsmall cell lung cancer cell lines developed from surgically resected tissues. Using various biochemical assays, we have seen that PJ34(TM) effects are consistent between untreated and treated samples but still somewhat variable between each cell line. Changes in protein expression and mitochondrial membrane potential between treated and untreated cells were indicating the possibility of apoptosis induction through an intrinsic pathway which causes cytotoxicity. Present results open the possibility of elucidating a decisive mechanism and effectiveness of chemotherapeutics specific to a patient.
authors.affiliation: Department of Cardiothoracic Surgery, and University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, PA, USA,1.
authors.forename: Nupur N
authors.initials: NN
authors.lastname: Gangopadhyay
authors.forename: James D
authors.initials: JD
authors.lastname: Luketich
authors.forename: Amy
authors.initials: A
authors.lastname: Opest
authors.forename: Rodney
authors.initials: R
authors.lastname: Landreneau
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Schuchert
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D045304:Cytochromes c / Q000096:biosynthesis; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis; D008175:Lung Neoplasms / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000502:physiology; D010616:Phenanthrenes / Q000494:pharmacology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D014176:Protein Biosynthesis / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D000077022:Survivin; D014407:Tumor Cells, Cultured; D014945:Wound Healing / Q000187:drug effects
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; C434926:N-(oxo-5,6-dihydrophenanthridin-2-yl)-N,N-dimethylacetamide hydrochloride; D010616:Phenanthrenes; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D000077022:Survivin; D045304:Cytochromes c; D053148:Caspase 3; D053453:Caspase 9
keywords: Lung cancer; Signal transduction; Therapy