title: Yolk sac mesenchymal progenitor cells from New World mice (Necromys lasiurus) with multipotent differential potential.
abstract: Fetal membranes are abundant, ethically acceptable and readily accessible sources of stem cells. In particular, the yolk sac is a source of cell lineages that do not express MHCs and are mainly free from immunological incompatibles when transferred to a recipient. Although data are available especially for hematopoietic stem cells in mice and human, whereas other cell types and species are dramatically underrepresented. Here we studied the nature and differentiation potential of yolk sac derived mesenchymal stem cells from a New World mouse, Necromys lasiurus. Explants from mid-gestation were cultured in DMEM-High glucose medium with 10% defined fetal bovine serum. The cells were characterized by standard methods including immunophenotyping by fluorescence and flow cytometry, growth and differentiation potential and tumorigenicity assays. The first adherent cells were observed after 7 days of cell culture and included small, elongated fibroblast-like cells (92.13%) and large, round epithelial-like cells with centrally located nuclei (6.5%). Only the fibroblast-like cells survived the first passages. They were positive to markers for mesenchymal stem cells (Stro-1, CD90, CD105, CD73) and pluripotency (Oct3/4, Nanog) as well as precursors of hematopoietic stem cells (CD117). In differentiation assays, they were classified as a multipotent lineage, because they differentiated into osteogenic, adipogenic, and chondrogenic lineages and, finally, they did not develop tumors. In conclusion, mesenchymal progenitor cells with multipotent differentiation potential and sufficient growth and proliferation abilities were able to be obtained from Necromys yolk sacs, therefore, we inferred that these cells may be promising for a wide range of applications in regenerative medicine.
authors.affiliation: School of Veterinary Medicine and Animal Science, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Phelipe Oliveira
authors.initials: PO
authors.lastname: Favaron
authors.affiliation: School of Veterinary Medicine and Animal Science, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mess
authors.affiliation: School of Veterinary Medicine and Animal Science, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Sônia Elisabete
authors.initials: SE
authors.lastname: Will
authors.affiliation: School of Veterinary Medicine and Animal Science, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Paulo César
authors.initials: PC
authors.lastname: Maiorka
authors.affiliation: Department of Animal Science, Universidade Federal Rural do Semi-Árido, Mossoró, Rio Grande do Norte, Brazil.
authors.forename: Moacir Franco
authors.initials: MF
authors.lastname: de Oliveira
authors.affiliation: School of Veterinary Medicine and Animal Science, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Maria Angelica
authors.initials: MA
authors.lastname: Miglino
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015197:Carcinogenicity Tests; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008819:Mice, Nude; D016205:Sigmodontinae / Q000502:physiology*; D015017:Yolk Sac / Q000166:cytology*
chemical_list: D015703:Antigens, CD