title: Construction of spherical liposome on solid transducers for electrochemical DNA sensing and transfection.
abstract: Cationic 1,2-dioleoyl trimethyl ammonium propane (DOTAP) and neutral 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) are anchored on cysteamine (cyst), mercaptopropionic acid (MPA) monolayer (thiol monolayers) modified on an individual gold transducer. DOTAP and DOPE are mixed with gold nanoparticle (AuNP) to form spherical liposome-AuNP. The electrochemical behaviors of the surface attached DOTAP-AuNP and DOPE-AuNP in presence of [Fe(CN)6](3-/4-) depend on the method of layer formation. Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and ultraviolet (UV)-visible spectroscopic techniques are used to characterize the liposome-AuNP nanocomposite. The studies indicate stability of spherical liposome-AuNP on the gold transducer. Label-free DNA hybridization detection on these surfaces reveals different detection limits. Confocal laser scanning microscopy (CLSM) is used to confirm the cell transfection.
authors.affiliation: Molecular Electronics Laboratory, Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi, 630 003, India.
authors.forename: Mohanlal
authors.initials: M
authors.lastname: Bhuvana
authors.forename: Venkataraman
authors.initials: V
authors.lastname: Dharuman
mesh_terms: D003543:Cysteamine / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D010714:Phosphatidylethanolamines / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D005229:Fatty Acids, Monounsaturated; D008055:Lipids; D008081:Liposomes; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D003543:Cysteamine; D006046:Gold; D004247:DNA; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane