title: [Evaluation of immunological efficiency induced by Campylobacter jejuni PEB1 DNA combined with PEB1 protein in mice].
abstract: OBJECTIVE
  To explore the improved immunological responses induced by an amino acid ABC transporter, permease protein PEB1 DNA vaccine primer-protein boost immunization method against Campylobacter jejuni.
  
  
  METHODS
  The DNA vaccine pcDNA3.1(-)-PEB1 and protein vaccine were prepared, respectively. The female BALB/c mice were intranasally immunized with the vaccines. PBS and pcDNA3.1(-) were used as controls. The humoral and cellular immunological responses were detected in female BALB/c mice that were challenged by Campylobacter jejuni at 28 days after the final immunization.
  
  
  RESULTS
  In the DNA primer-protein boost group at day 56, the stimulation index (SI) of lymphocytes was 2.625±0.275, serum IgG was (2.507±0.124) μg/mL, IL-4 in spleen supernatant was (377.47±14.560) pg/mL, IFN-γ in spleen supernatant was (258.920±13.472) pg/mL, and sIgA in genital tract was (80.351±5.769) ng/mL. All of them were significantly higher than those in controls (P<0.05). The DNA primer-protein boost vaccines induced the strongest levels of protection to BALB/c mice (91.53%).
  
  
  CONCLUSIONS
  The DNA primer-protein boost immunization could induce significant protective immunity against Campylobacter jejuni challenge. It could significantly enhance both humoral and cellular immunologic responses in BALB/c mice, compared with DNA vaccine or protein vaccine immunization alone.
authors.affiliation: Department of Pathogen Biology and Immunology, Shandong Medical College, Linyi 276002, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pathogen Biology and Immunology, Shandong Medical College, Linyi 276002, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Lai
authors.affiliation: Department of Pathogen Biology and Immunology, Shandong Medical College, Linyi 276002, China.
authors.forename: Na
authors.initials: N
authors.lastname: Hu
authors.affiliation: Department of Pathogen Biology and Immunology, Shandong Medical College, Linyi 276002, China.
authors.forename: Weizhe
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Pathogen Biology and Immunology, Shandong Medical College, Linyi 276002, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Pathogen Biology and Immunology, Shandong Medical College, Linyi 276002, China.
authors.forename: Faquan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Pathogen Biology and Immunology, Shandong Medical College, Linyi 276002, China.
authors.forename: Runguo
authors.initials: R
authors.lastname: Gu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D002169:Campylobacter Infections / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D016123:Campylobacter jejuni / Q000276:immunology* / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D054884:Host-Pathogen Interactions / Q000276:immunology; D007114:Immunization / Q000379:methods; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C489977:PEB1 protein, Campylobacter; D019444:Vaccines, DNA; D022223:Vaccines, Subunit; D015847:Interleukin-4; D007371:Interferon-gamma