title: A novel phage-library-selected peptide inhibits human TNF-α binding to its receptors.
abstract: We report the identification of a new human tumor necrosis factor-alpha (TNF-α) specific peptide selected by competitive panning of a phage library. Competitive elution of phages was obtained using the monoclonal antibody adalimumab, which neutralizes pro-inflammatory processes caused by over-production of TNF-α in vivo, and is used to treat severe symptoms of rheumatoid arthritis. The selected peptide was synthesized in monomeric and branched form and analyzed for binding to TNF-α and competition with adalimumab and TNF-α receptors. Results of competition with TNF-α receptors in surface plasmon resonance and melanoma cells expressing both TNF receptors make the peptide a candidate compound for the development of a novel anti-TNF-α drug.
authors.affiliation: Department of Medical Biotechnologies, University of Siena, Via A. Moro 2, 53100 Siena, Italy. jlenia.brunetti@unisi.it.
authors.forename: Jlenia
authors.initials: J
authors.lastname: Brunetti
authors.affiliation: Department of Medical Biotechnologies, University of Siena, Via A. Moro 2, 53100 Siena, Italy. barbara.lelli@unisi.it.
authors.forename: Barbara
authors.initials: B
authors.lastname: Lelli
authors.affiliation: Department of Medical Biotechnologies, University of Siena, Via A. Moro 2, 53100 Siena, Italy. silvia.scali@unisi.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Scali
authors.affiliation: SetLance srl, via Fiorentina 1, 53100 Siena, Italy. falciani@setlance.com.
authors.forename: Chiara
authors.initials: C
authors.lastname: Falciani
authors.affiliation: Department of Medical Biotechnologies, University of Siena, Via A. Moro 2, 53100 Siena, Italy. luisa.bracci@unisi.it.
authors.forename: Luisa
authors.initials: L
authors.lastname: Bracci
authors.affiliation: Department of Medical Biotechnologies, University of Siena, Via A. Moro 2, 53100 Siena, Italy. alessandro.pini@unisi.it.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pini
mesh_terms: D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D060327:Solid-Phase Synthesis Techniques; D020349:Surface Plasmon Resonance; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D019151:Peptide Library; D010455:Peptides; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha