title: Protective effects of osthole on intestinal ischemia-reperfusion injury in mice.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the effect of intravenous injection of osthole on intestinal ischemia-reperfusion injury and parameters of oxidative stress.
  
  
  METHODS
  In 45 Kunming male mice, treatment included sham surgery (15 mice); intestinal ischemia-reperfusion injury (clamping of the superior mesenteric artery, 2 h; clamp release, 1 h; 15 mice); or osthole treatment before and after ischemia-reperfusion injury (15 mice). Evaluation included histopathology, determination of intestinal wet/dry weight ratio, and measurement of levels of diamine oxidase, superoxide dismutase, malondialdehyde, interleukin 1β, tumor necrosis factor α, and interleukin 2. Intestinal barrier permeability was evaluated with Evans blue test.
  
  
  RESULTS
  The mean wet-to-dry weight ratio, Evans blue content, and Chiu score were significantly greater in the ischemia-reperfusion than in the sham group and lower in the osthole-treated than the ischemia-reperfusion group. The mean serum diamine oxidase, malondialdehyde, interleukin 1β, and tumor necrosis factor α levels were significantly greater in the ischemia-reperfusion than in the sham group and lower in the osthole-treated than in the ischemia-reperfusion group. The mean superoxide dismutase activity and interleukin 2 levels were lower in the ischemia-reperfusion than in the sham group and greater in the osthole-treated than in the ischemia-reperfusion group.
  
  
  CONCLUSIONS
  Treatment with osthole may protect against oxidative stress and tissue damage from intestinal ischemia-reperfusion injury.
authors.affiliation: Endoscopy Center, Department of Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Chen
authors.initials: C
authors.lastname: Pan
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Wang
authors.forename: Yong-xiang
authors.initials: YX
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D003374:Coumarins / Q000008:administration & dosage / Q000494:pharmacology*; D019610:Cytoprotection; D004195:Disease Models, Animal; D007082:Ileum / Q000098:blood supply* / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007275:Injections, Intravenous; D008297:Male; D065666:Mesenteric Ischemia / Q000097:blood / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D008641:Mesenteric Vascular Occlusion / Q000097:blood / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010539:Permeability; D015427:Reperfusion Injury / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D003374:Coumarins; C046627:osthol