title: Experimental and theoretical investigations of Lantana camara oil diffusion from polyacrylonitrile membrane for pulsatile drug delivery system.
abstract: Porous composite membrane of polyacrylonitrile (PAN) and Lantana camara essential oil was synthesized by solvent casting method. Stability of oil in PAN solution was measured by XiGo nano tool indicating constant relaxation time of 1487 time/s. Pore size of few microns confirmed by electron microscopy was supported by atomic force microscopy indicating roughness factor of 0.9 nm. Contact angle of 2° inveterates superhydrophilicity of the composite membrane. Membrane showed excellent antibacterial activity against both Gram-positive Bacillus subtilis and Gram-negative Escherichia coli with a 7-10mm zone of inhibition. In vitro release of Lantana oil from the composite membrane was carried out in isotonic phosphate buffer solution (pH=7.4). Lantana oil was released for 9h, lag time of 3h with constant 33% release confirmed PAN membranes as potential system for pulsatile drug delivery applications. Diffusion of E-caryophyllene (antibacterial component of oil) which was studied through molecular simulation using Material Studio software ensued diffusion coefficient value of 1.11∗10(-9) m(2)/s. Biocompatibility of the composite membrane was assessed by mouse embryonic fibroblast cell line (NIH 3T3) through MTT assay indicating more than 91% viable cell even at 200 μg/mL concentration. Such membranes can be efficiently used in biomedical applications as antibacterial and antifungal agent.
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER), Pune 411008, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Verma
authors.affiliation: Department of Materials Engineering, Defence Institute of Advance Technology (DIAT), Ministry of Defence, Girinagar, Pune 411025, India. Electronic address: meetkbs@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Balasubramanian
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D032410:Lantana / Q000737:chemistry* / Q000378:metabolism; D008567:Membranes, Artificial; D051379:Mice; D008826:Microbial Sensitivity Tests; D056004:Molecular Dynamics Simulation; D041681:NIH 3T3 Cells; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D016062:Porosity
chemical_list: D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008567:Membranes, Artificial; D009822:Oils, Volatile; C010504:polyacrylonitrile
keywords: Antibacterial; Lantana camara; Polyacrylonitrile; Pulsatile release; Superhydrophilic