title: Protein kinases paralleling late-phase LTP formation in dorsal hippocampus in the rat.
abstract: Hippocampal long term potentiation (LTP), representing a cellular model for learning and memory formation, can be dissociated into at least two phases: a protein-synthesis-independent early phase, lasting about 4h and a protein-synthesis-dependent late phase LTP lasting 6h or longer, or even days. A large series of protein kinases have been shown to be involved and herein, a distinct set of protein kinases proposed to be involved in memory retrieval in previous work was tested in dorsal hippocampus of the rat following induction of late-phase LTP. A bipolar stimulation electrode was chronically implanted into the perforant path, while two monopolar recording electrodes were implanted into the dentate gyrus of the dorsal hippocampus. The recording electrode was measuring extracellular excitatory postsynaptic potentials, while the other one measured population spikes. Protein kinases were determined by immunoblotting and immunoflourescence on hippocampal areas showed the distribution pattern of protein kinases PKN1 and NEK7. Induction of LTP was proven, elevated levels for protein kinases PKN1, RPS6KB1, STK4, CDC42BPB, PRKG, TLK, BMX and decreased levels for NEK7, MAK14 and PLK1 were observed. A remarkable overlap of protein kinases observed in spatial memory processes with those proposed in LTP formation was demonstrated. The findings may be relevant for design of future studies on protein kinases and for the interpretation of previous work.
authors.affiliation: Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Jia
authors.initials: J
authors.lastname: Wan
authors.affiliation: Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Sunetra
authors.initials: S
authors.lastname: Sase
authors.affiliation: CF Imaging, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Marion
authors.initials: M
authors.lastname: Gröger
authors.affiliation: Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Arnold
authors.initials: A
authors.lastname: Pollak
authors.affiliation: Institute of Biology, Otto von Guericke University Magdeburg, 39120 Magdeburg, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Korz
authors.affiliation: Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria. Electronic address: gert.lubec@meduniwien.ac.at.
authors.forename: Gert
authors.initials: G
authors.lastname: Lubec
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004566:Electrodes; D019706:Excitatory Postsynaptic Potentials; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000201:enzymology / Q000502:physiology*; D017774:Long-Term Potentiation; D008297:Male; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011494:Protein Kinases
keywords: Dorsal hippocampus; Kinase network; LTP; Memory; Protein kinase; Signalling