title: Characterization and cytocompatibility of an antibiotic/chitosan/cyclodextrins nanocoating on titanium implants.
abstract: A novel ciprofloxacin loaded chitosan nanoparticle-based coating onto titanium substrates has been developed and characterized to obtain an orthopaedic implant surface able to in situ release the antibiotic for the prevention of post-operative infections. Ciprofloxacin loaded chitosan nanoparticles were obtained using the combination of sulfobutyl ether-beta-cyclodextrin and gamma-cyclodextrin. The resulting nanoparticulate system was characterized by TEM, HPLC and XPS. Particle size was in the range 426-552 nm and zeta potential values were around +30 mV. This antibacterial coating was able to in vitro inhibit two nosocomial Staphylococcus aureus strains growth, with a reduction of about 20 times compared to controls. No impairment in MG63 osteoblast-like cells viability, adhesion and gene expression were detected at 48 h, 7 and 14 days of culture. Overall, the investigated coating represents a promising candidate for the development of a new antibiotic carrier for titanium implants.
authors.affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, Via Tronto 10/A, 60126 Ancona, Italy. Electronic address: m.mattioli@univpm.it.
authors.forename: Monica
authors.initials: M
authors.lastname: Mattioli-Belmonte
authors.affiliation: Jaber Innovation s.r.l., Via Calcutta 8, 00100 Roma, Italy. Electronic address: stefania.cometa@virgilio.it.
authors.forename: Stefania
authors.initials: S
authors.lastname: Cometa
authors.affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, Via Tronto 10/A, 60126 Ancona, Italy. Electronic address: c.ferretti@univpm.it.
authors.forename: Concetta
authors.initials: C
authors.lastname: Ferretti
authors.affiliation: Department of Veterinary Medicine, University of Bari Aldo Moro, Str. Prov. per Casamassima Km 3, Valenzano (BA), Italy. Electronic address: iroberta@hotmail.com.
authors.forename: Roberta
authors.initials: R
authors.lastname: Iatta
authors.affiliation: Department of Pharmacy-Drug Sciences, University of Bari Aldo Moro, Via E. Orabona 4, 70126 Bari, Italy. Electronic address: adriana.trapani@uniba.it.
authors.forename: Adriana
authors.initials: A
authors.lastname: Trapani
authors.affiliation: Department of Veterinary Medicine, University of Bari Aldo Moro, Str. Prov. per Casamassima Km 3, Valenzano (BA), Italy. Electronic address: edmondo.ceci@uniba.it.
authors.forename: Edmondo
authors.initials: E
authors.lastname: Ceci
authors.affiliation: Department of Biomedical and Neuromotor Sciences, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy. Electronic address: mirella.falconi@unibo.it.
authors.forename: Mirella
authors.initials: M
authors.lastname: Falconi
authors.affiliation: Department of Chemistry, University of Bari Aldo Moro, Via E. Orabona 4, 70126 Bari, Italy. Electronic address: elvira.degiglio@uniba.it.
authors.forename: Elvira
authors.initials: E
authors.lastname: De Giglio
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D019736:Prostheses and Implants / Q000009:adverse effects; D016459:Prosthesis-Related Infections / Q000517:prevention & control*; D013203:Staphylococcal Infections / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000187:drug effects; D014025:Titanium / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry; D047408:gamma-Cyclodextrins / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; C093196:SBE4-beta-cyclodextrin; D002939:Ciprofloxacin; D048271:Chitosan; D014025:Titanium; C023792:gamma-cyclodextrin
keywords: Chitosan nanoparticles; Ciprofloxacin; Cyclodextrins; Cytocompatibility; Gene expression; Orthopaedic infections