title: Effects of splenic allograft in lipid profile of non-splenectomized rats: the immune and metabolic role of the "double spleen".
abstract: OBJECTIVE
  To elucidate the role of the spleen and splenic allograft in lipid control and evaluate its effect on the lipid profile of rats.
  
  
  METHODS
  32 male Wistar rats were randomly assigned into four groups: control group (1), total splenectomy group (2), splenectomy and implantation of allograft group (3) and double spleen group (4). Each group was subdivided into two subgroups: A and B, based on the death of the animals after 30 or 120 days of monitoring. The procedures in groups 2, 3 and 4 were made simultaneously, and splenectomized animals, groups 2 and 3 were donors, respectively, for the animals of groups 3 and 4. In group 4 the spleen was preserved and the animals received implants from the spleens of rats from group 3. The regeneration of splenic tissue was evaluated by macroscopic and microscopic analyzes of the grafts and own spleens, as well as with measurements of VLDL, HDL, LDL, total cholesterol and triglycerides.
  
  
  RESULTS
  after 120 days, Group 4 showed levels of total cholesterol and LDL lower than the other groups. Group 1 had higher levels of lipids.
  
  
  CONCLUSIONS
  The technique of double spleen was effective in the control of lipid metabolism, corroborating the function of the spleen as a reserve of lipids.
authors.affiliation: Faculty of Medicine, State University of Pará.
authors.forename: Thiago Barbosa
authors.initials: TB
authors.lastname: Gonçalves
authors.affiliation: Faculty of Medicine, State University of Pará.
authors.forename: Vitor Nagai
authors.initials: VN
authors.lastname: Yamaki
authors.affiliation: Faculty of Medicine, State University of Pará.
authors.forename: Daniel Haber
authors.initials: DH
authors.lastname: Feijó
authors.affiliation: Faculty of Medicine, State University of Pará.
authors.forename: Luis Eduardo Almeida de
authors.initials: LE
authors.lastname: Souza
authors.affiliation: Department of General Pathology, Faculty of Medicine, State University of Pará.
authors.forename: Edvaldo
authors.initials: E
authors.lastname: Silveira
authors.affiliation: Discipline of Operative Technique, Faculty of Medicine, State University of Pará.
authors.forename: Marcus Vinicius Henriques
authors.initials: MV
authors.lastname: Brito
authors.affiliation: Department of Surgery, Faculty of Medicine, Federal University of Minas Gerais.
authors.forename: Andy
authors.initials: A
authors.lastname: Petroianu
mesh_terms: D064591:Allografts / Q000276:immunology* / Q000378:metabolism*; D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000276:immunology* / Q000378:metabolism* / Q000637:transplantation; D013156:Splenectomy; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D014280:Triglycerides; D002784:Cholesterol