title: Structural and functional analysis of various globulin proteins from soy seed.
abstract: Storage proteins of soybean mostly consist of globulins, which are classified according to their sedimentation coefficient. Among 4 major types: 2S, 7S, 11S, and 15S of globulins, 7S and 11S constitute major fraction. The 11S fraction consists only of glycinin and 7S fraction majorly consists of β-conglycinin, small amounts of γ-conglycinin and basic 7S globulin (Bg7S). Glycinin exist as a hexamer while β-conglycinin as a trimer and Bg7S as a tetramer. Glycinin subunits are coded by 5 genes of a family, whereas about 15 genes are present for β-conglycinin subunits. Bg7S gene is present in four copies in soybean genome. Synthesis of all proteins takes place as a single polypeptide chain, which is cleaved after folding to yield different chains or subunits. Glycinin and β-Conglycinin are made for storage purpose. However, Bg7S has potential xylanase inhibition activity and protein kinase activity. Primary structure of Bg7S reveals 12 conserved cysteine residues involved in forming 6 disulfide bonds, which provides appreciable stability to protein. Secondary structure is predominately rich in β-sheets with few alpha helices. Bg7S shares structural similarity with various aspartic-proteases. In this review, our aim is to discuss sequence, structure, and function of various globulins present in Glycine max.
authors.affiliation: a Division of Biotechnology, Netaji Subhas Institute of Technology , Azad Hind Fauz Marg, Sector-3, Dwarka, New Delhi , India.
authors.forename: Amandeep
authors.initials: A
authors.lastname: Singh
authors.forename: Megha
authors.initials: M
authors.lastname: Meena
authors.forename: Dhiraj
authors.initials: D
authors.lastname: Kumar
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Dubey
authors.forename: Md Imtaiyaz
authors.initials: MI
authors.lastname: Hassan
mesh_terms: D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000032:analysis / Q000737:chemistry; D005916:Globulins / Q000032:analysis / Q000737:chemistry; D055314:Seed Storage Proteins / Q000032:analysis / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D030262:Soybean Proteins / Q000032:analysis* / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D052179:Antigens, Plant; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C011474:glycinin
keywords: 11S and 7S globulin; Soybean globulins; basic 7S globulin; hypo-cholesterolemic effect; β-Conglycinin