title: Dual role of the cuttlefish salivary proteome in defense and predation.
abstract: We characterized the proteome of the posterior salivary glands of the cephalopod S. officinalis by combining de novo RNA sequencing and mass spectrometry. In silico analysis of the transcriptome revealed the occurrence of three main categories of proteins: enzymes, immune factors and toxins. Protein identification by SDS-PAGE and MALDI-TOF/TOF confirmed the occurrence of proteins essential to venom-like enzymes: peptidase S1 under four isoforms, phospholipase A2 and two toxins. The first toxin is a cystein rich secreted protein (CRISP), a common toxin found in all venomous animals. The second one is cephalotoxin, which is specific to decabrachia cephalopods. Secretions of the posterior salivary glands are transported to the cephalopodium; they are involved in prey catching but also in gamete storage, fertilization and egg-laying. The paralyzing activity and the antimicrobial effect of saliva suggest a dual role in predation and in immune defense in cuttlefish.
  
  
  CONCLUSIONS
  The originality of this study lies in the use of a transcriptomic approach (de novo RNA sequencing) coupled to a proteomic approach to get an overview of posterior salivary glands in S. officinalis. In cephalopods, these glands are involved in predation, more precisely in paralyzing preys and digesting them. Our in silico analysis equally reveals a role in immune defense as observed in mammals' saliva. Our study also shows the specificity of cuttlefish venom, with the identification of cephalotoxins, proteins that are not found in octopuses. Finally, we show that cuttlefish saliva is a complex mixture that has antibacterial and crippling properties, but no lethal effect.
authors.affiliation: Université de Caen Basse-Normandie, IBFA, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, CNRS-7208, IRD-207, UCBN, F-14032 Caen, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Cornet
authors.affiliation: Université de Caen Basse-Normandie, IBFA, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, CNRS-7208, IRD-207, UCBN, F-14032 Caen, France; Post Genomic Platform PROTEOGEN, Université de Caen Basse-Normandie, F-14032 Caen, France.
authors.forename: Joël
authors.initials: J
authors.lastname: Henry
authors.affiliation: ABiMs Platform, Station biologique de Roscoff (UPMC-CNRS), F-29688 Roscoff, France.
authors.forename: Erwan
authors.initials: E
authors.lastname: Corre
authors.affiliation: ABiMs Platform, Station biologique de Roscoff (UPMC-CNRS), F-29688 Roscoff, France.
authors.forename: Gildas
authors.initials: G
authors.lastname: Le Corguille
authors.affiliation: Université de Caen Basse-Normandie, GREYC, UMR 6072, UNICAEN, CNRS, ENSICAEN, F-14032 Caen, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Zanuttini
authors.affiliation: Université de Caen Basse-Normandie, IBFA, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, CNRS-7208, IRD-207, UCBN, F-14032 Caen, France. Electronic address: celine.gaudin@unicaen.fr.
authors.forename: Céline
authors.initials: C
authors.lastname: Zatylny-Gaudin
mesh_terms: D000818:Animals; D049832:Decapodiformes / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D011235:Predatory Behavior; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome; D012471:Salivary Proteins and Peptides
keywords: Immune defense; Predation; Proteome; Saliva; Sepia officinalis; Venom