title: Influence of lipoic acid on testicular toxicity induced by bi-n-butyl phthalate in rats.
abstract: Bi-n-butyl phthalate (BNBP) is an environmental pollutant. The aim of this study was to evaluate the protective effect of lipoic acid (LA) against testicular dysfunction associated with the intake of to BNBP- intoxicated rats. Adult male Wistar rats were divided into 4 groups of 6 animals each, and received medication orally for 14 days. Group I rats received 0.5 ml corn oil. Group II rats received LA (20 mg/kg B.W./day). Group III rats received BNBP (250 mg/kg B.W./day). Group IV rats received LA 24h prior to BNBP intake. Testes weight, cauda sperm count and sperm motility were decreased significantly by 18.15%, 13.83% and 13.5%, respectively, after BNBP treatment. Significant increase by 12.1%, 10.20% and 11.51%, respectively, was observed in LA-BNBP rats. Significant increase by 1.53%, 1.5% and 1.8%, for serum follicle stimulating hormone, testosterone and total antioxidant status, respectively, were observed in LA-BNBP rats. Testicular lipid peroxides and lactate dehydrogenase enzyme were significantly decreased by 1.5 and 1.6 folds, respectively, in LA-BNBP rats were decreased after BNBP treatment. Testicular superoxide dismutase, catalase and glutathione reductase enzymes were significantly increased in LA-BNBP rats. LA-BNBP rats, decreased the damage to seminiferous tubules produced by BNBP intake. In conclusion, LA mitigated BNBP-induced testicular toxicity through antioxidant mechanism and by direct free radical scavenging activity.
authors.affiliation: Medical Laboratories Technology Department, Faculty of Applied Medical Sciences, Taibah University, Madinah, Saudi Arabia; Biochemistry Department, Al-Azhar University, Nasr City, Cairo 11751, Egypt. Electronic address: hesham_elbeshbishy@hotmail.com.
authors.forename: Hesham A
authors.initials: HA
authors.lastname: El-Beshbishy
authors.affiliation: Biochemistry and Molecular Medicine Department, College of Medicine, Taibah University, Saudi Arabia; Medical Biochemistry Department, Faculty of Medicine, Tanta University, Egypt.
authors.forename: Reham A
authors.initials: RA
authors.lastname: Mariah
authors.affiliation: Biochemistry and Molecular Medicine Department, College of Medicine, Taibah University, Saudi Arabia; Clinical Pathology Department, National Cancer Institute, Cairo University, Egypt.
authors.forename: Nevin M
authors.initials: NM
authors.lastname: Al-Azhary
authors.affiliation: Medical Biochemistry Department, Faculty of Medicine, Tanta University, Egypt; Pharmacology &Toxicology Department, King AbdulAziz University, Jeddah, Saudi Arabia.
authors.forename: Hamdy A A
authors.initials: HA
authors.lastname: Aly
authors.affiliation: Medical Laboratories Technology Department, Faculty of Applied Medical Sciences, Taibah University, Madinah, Saudi Arabia.
authors.forename: Hani A
authors.initials: HA
authors.lastname: Ozbak
authors.affiliation: Biochemistry and Molecular Medicine Department, College of Medicine, Taibah University, Saudi Arabia.
authors.forename: Hussam H
authors.initials: HH
authors.lastname: Baghdadi
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D003993:Dibutyl Phthalate / Q000633:toxicity*; D005640:Follicle Stimulating Hormone / Q000097:blood; D005980:Glutathione Reductase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000201:enzymology; D013739:Testosterone / Q000097:blood; D008063:Thioctic Acid / Q000494:pharmacology*
chemical_list: D003993:Dibutyl Phthalate; D013739:Testosterone; D008063:Thioctic Acid; D005640:Follicle Stimulating Hormone; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase
keywords: Antioxidant; Bi-n-butyl phthalate; Lipoic acid; Oxidative stress; Testes