title: Molecular characterization of diverse species enterovirus-B types from children with acute flaccid paralysis and asymptomatic children in Nigeria.
abstract: Non-polio enteroviruses (NPEVs) have often been identified in association with acute flaccid paralysis (AFP) in most polio surveillance studies worldwide. In a polio endemic country like Nigeria, there is need for distinction of AFP due to poliovirus and those potentially due to NPEVs. This study was undertaken to characterize the enterovirus (EV) types circulating in both children with and without AFP in Nigeria. Of fecal sample from 966 children with AFP, 96 (10%) were positive for NPEVs in RD cells, while 42 (5.5%) of 756 samples from non-AFP children was positive. Genotyping of all NPEV isolates was done by partial VP1 gene sequencing and phylogenetic analysis. EV-B was the predominant species detected (84%) and infection was common in children with AFP with CVB3, E6, and E11 constituting the predominant types detected. The CVB3 isolates cluster with Chinese CVB3 isolate recently detected in a newborn with AFP. There was also a remarkable clustering of isolates such as E6, E12, E13, E24, E30 and E33 to types previous detected in West Africa suggesting a probable circulation of these lineages in the region. Taken together, this study reveals a diverse species EV-B types in AFP cases and highlights the fact that other neurotropic EVs circulate in asymptomatic persons. Improved continuous surveillance of NPEV is warranted as in the likely attainment of polio eradication, other neurotropic EVs may emerge causing similar paralytic diseases.
authors.affiliation: Institute for Advanced Medical Research and Training, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: Olufunmilayo G
authors.initials: OG
authors.lastname: Oyero
authors.affiliation: Department of Virology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: Festus D
authors.initials: FD
authors.lastname: Adu
authors.affiliation: Department of Infectious Diseases/Section of Clinical Virology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden. Electronic address: ayukjim@yahoo.com.
authors.forename: James A
authors.initials: JA
authors.lastname: Ayukekbong
mesh_terms: D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009549:Nigeria; D010264:Paraplegia / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D015678:Viral Structural Proteins
keywords: Acute flaccid paralysis; Enterovirus; Nigeria; Non-polio enterovirus; VP1 typing