title: Differentially expressed proteins in human breast cancer cells sensitive and resistant to paclitaxel.
abstract: The resistance of cancer cells to chemotherapeutic drugs represents a major problem in cancer treatment. Despite all efforts, mechanisms of resistance have not yet been elucidated. To reveal proteins that could be involved in resistance to taxanes, we compared protein expression in whole cell lysates of SK-BR-3 breast cancer cells sensitive to paclitaxel and in lysates of the same line with acquired resistance to paclitaxel. The resistant SK-BR-3 cell line was established in our lab. Protein separation was achieved using high-resolution 2D-electrophoresis, computer analysis and mass spectro-metry. With these techniques we identified four proteins with different expression in resistant SK-BR-3 cells, i.e., serpin B3, serpin B4, heat shock protein 27 (all three upregulated) and cytokeratin 18 (downregulated). Observed changes were confirmed using western blot analysis. This study suggests new directions worthy of further study in the effort to reveal the mechanism of resistance to paclitaxel in breast cancer cells.
authors.affiliation: Department of Cellular and Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Nela
authors.initials: N
authors.lastname: Pavlikova
authors.affiliation: Department of Cellular and Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Irena
authors.initials: I
authors.lastname: Bartonova
authors.affiliation: Department of Cellular and Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Lucia
authors.initials: L
authors.lastname: Dincakova
authors.affiliation: Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Halada
authors.affiliation: Department of Cellular and Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Kovar
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000096:biosynthesis; D006801:Humans; D053538:Keratin-18 / Q000096:biosynthesis*; D017239:Paclitaxel / Q000494:pharmacology; D040901:Proteomics; D015843:Serpins / Q000096:biosynthesis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000951:Antigens, Neoplasm; D000972:Antineoplastic Agents, Phytogenic; D055551:HSP27 Heat-Shock Proteins; D053538:Keratin-18; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; D017239:Paclitaxel