title: Nanostructuring of hierarchical 3D cystine flowers for high-performance electrochemical immunosensor.
abstract: Here, we report a simple and reproducible method for large scale fabrication of novel flower and palm-leaf like 3D cystine microstructures (CMs) with high uniformity having a size of 50 µm and 10 µm respectively, through a facile aqueous solution route as a function of pH and concentration. In a proof-of-concept study, the 3D CMs have been further explored to fabricate a label-free high-performance electrochemical immunosensor by immobilizing monoclonal antibodies. Electrochemical methods were employed to study the stepwise modification of the system and the electronic transduction for the detection. The fabricated immunosensor design demonstrates high performance with enhanced sensitivity (4.70 cfu ml(-1)) and linear sensing range from 10 to 3 x 10(9) cfu ml(-1) a long shelf-life (35 days) and high selectivity over other bacterial pathogens. The enhanced performance originates from a novel nanostructuring in which the CMs provide higher surface coverage for the immobilization of antibodies providing excellent electronic/ionic conductivity which result in the enhanced sensitivity.
authors.affiliation: (a)Biomedical Instrumentation Section, CSIR- National Physical Laboratory, New Delhi 110012, India; (b)Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Chandra Mouli
authors.initials: CM
authors.lastname: Pandey
authors.affiliation: (a)Biomedical Instrumentation Section, CSIR- National Physical Laboratory, New Delhi 110012, India. Electronic address: sumanagajjala@gmail.com.
authors.forename: Gajjala
authors.initials: G
authors.lastname: Sumana
authors.affiliation: (b)Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Ida
authors.initials: I
authors.lastname: Tiwari
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003553:Cystine / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D003553:Cystine
keywords: Cystine; Electrochemical; Immunosensor; Nanostructuring; Organic flowers