title: A method for measuring difference in activity of phenolic and non- phenolic groups through 2,2` azino-bis-(3-ethyl-benzothiazoline-6- sulphonate) radical cation.
abstract: A 2, 2`azino-bis-(3-ethyl-benzothiazoline-6-sulphonate) radical cation-based method was optimized in 96 well plates to evaluate the comparative scavenging potential of phenolic and non-phenolic group of molecules with respect to control. The interactions of these molecules with ABTS radical cation were quantified on the basis of their relative influence on the bleaching of a bluish-green color complex in a structure- and dose-dependent manner. Experimentally, the developed assay provided evidence that phenolic molecules are more reactive with ABTS radical than non-phenolic compounds because of their resonance and hyper-conjugation effects.
authors.forename: Nusrat
authors.initials: N
authors.lastname: Masood
authors.affiliation: Molecular Bioprospection Department, Biotechnology Division, CSIR-Central Institute of Medicinal & Aromatic Plants, Lucknow-226015, India. s.luqman@cimap.res.in.
authors.forename: Suaib
authors.initials: S
authors.lastname: Luqman
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005609:Free Radicals / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D005609:Free Radicals; D010636:Phenols; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C058836:benzothiazoline