title: Metabolite characterisation in peritoneal dialysis effluent using high-resolution (1) H and (1) H-(13) C NMR spectroscopy.
abstract: Metabolite analysis of peritoneal dialysis (PD) effluent may provide information regarding onset and progression of complications associated with prolonged PD therapy. In this context, the nuclear magnetic resonance detectable small metabolites of PD effluent samples were characterised using high-resolution (1) H and (1) H-(13) C NMR spectroscopy. The various spectra were recorded (at 800 MHz proton frequency) on PD effluent samples obtained after 4-h (intraperitoneal) dwell time from patients with end-stage renal failure and continuing normally on PD therapy. In spite of devastating spectral feature of PD effluent due to the presence of intense resonances from glucose and lactate, we were able to identify 53 small endogenous metabolites (including many complex coupled spin systems) and more than 90% of the total CH cross peaks of (1) H-(13) C heteronuclear single-quantum correlation spectrum specific to various metabolites of PD effluent. We foresee that the characteristic fingerprints of various metabolites of control PD effluent samples will be used to identify and distinguish metabolic differences from PD-related complications.
authors.affiliation: Centre of Biomedical Research, Lucknow, Uttar Pradesh, India.
authors.forename: Anupam
authors.initials: A
authors.lastname: Guleria
authors.forename: Nitin Kumar
authors.initials: NK
authors.lastname: Bajpai
authors.forename: Atul
authors.initials: A
authors.lastname: Rawat
authors.forename: C L
authors.initials: CL
authors.lastname: Khetrapal
authors.forename: Narayan
authors.initials: N
authors.lastname: Prasad
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
mesh_terms: D001774:Blood Chemical Analysis; D005240:Feasibility Studies; D005947:Glucose / Q000032:analysis; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism*; D019344:Lactic Acid / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010530:Peritoneal Dialysis / Q000009:adverse effects; D014556:Urine / Q000737:chemistry
chemical_list: D019344:Lactic Acid; D005947:Glucose
keywords: NMR; metabolites; pd effluent; peritoneal dialysis; peritonitis