title: Potentialities of coupling biological processes (biotrickler/biofilter) for the degradation of a mixture of sulphur compounds.
abstract: This study deals with the potential of biological processes combining a biotrickler and a biofilter to treat a mixture of sulphur-reduced compounds including dimethyl sulphide (DMS), dimethyl disulphide (DMDS) and hydrogen sulphide (H2S). As a reference, duplicated biofilters were implemented, and operating conditions were similar for all bioprocesses. The first step of this work was to determine the efficiency removal level achieved for each compound of the mixture and in a second step, to assess the longitudinal distribution of biodegradation activities and evaluate the total bacteria, Hyphomicrobium sp. and Thiobacillus thioparus densities along the bed height. A complete removal of hydrogen sulphide is reached at the start of the experiment within the first stage (biotrickler) of the coupling. This study highlighted that the coupling of a biotrickling filter and a biofilter is an interesting way to improve both removal efficiency levels (15-20% more) and kinetics of recalcitrant sulphur compounds such as DMS and DMDS. The total cell densities remained similar (around 1 × 10(10) 16S recombinant DNA (rDNA) copies g dry packing material) for duplicated biofilters and the biofilter below the biotrickling filter. The relative abundances of Hyphomicrobium sp. and T. thioparus have been estimated to an average of 10 ± 7.0 and 0.23 ± 0.07%, respectively, for all biofilters. Further investigation should allow achieving complete removal of DMS by starting the organic sulphur compound degradation within the first stage and surveying microbial community structure colonizing this complex system.
authors.affiliation: Ecole des mines d'Alès, 6 Avenue de Clavières, 30319, Alès Cedex, France, luc.malhautier@mines-ales.fr.
authors.forename: Luc
authors.initials: L
authors.lastname: Malhautier
authors.forename: Alexandre
authors.initials: A
authors.lastname: Soupramanien
authors.forename: Sandrine
authors.initials: S
authors.lastname: Bayle
authors.forename: Janick
authors.initials: J
authors.lastname: Rocher
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Fanlo
mesh_terms: D061808:Air Filters; D058491:Bacterial Load; D004220:Disulfides / Q000378:metabolism*; D005374:Filtration / Q000379:methods*; D006862:Hydrogen Sulfide / Q000378:metabolism*; D020582:Hyphomicrobium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013440:Sulfides / Q000378:metabolism*; D013855:Thiobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D004220:Disulfides; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; C021181:dimethyl disulfide; C004784:dimethyl sulfide; D006862:Hydrogen Sulfide