title: Comparing amyloid-β deposition, neuroinflammation, glucose metabolism, and mitochondrial complex I activity in brain: a PET study in aged monkeys.
abstract: OBJECTIVE
  The aim of the present study was to compare amyloid-β (Aβ) deposition, translocator protein (TSPO) activity, regional cerebral metabolic rate of glucose (rCMRglc), and mitochondrial complex I (MC-I) activity in the brain of aged monkeys.
  
  
  METHODS
  PET scans with (11)C-PIB (Aβ), (18)F-BCPP-EF (MC-I), (11)C-DPA-713 (TSPO), and (18)F-FDG (rCMRglc) were performed in aged monkeys (Macaca mulatta) in the conscious state and under isoflurane anaesthesia. (11)C-PIB binding to Aβ and (11)C-DPA-713 binding to TSPO were evaluated in terms of standard uptake values (SUV). The total volume of distribution (V T) of (18)F-BCPP-EF and rCMRglc with (18)F-FDG were calculated using arterial blood sampling.
  
  
  RESULTS
  Isoflurane did not affect MC-I activity measured in terms of (18)F-BCPP-EF uptake in living brain. There was a significant negative correlation between (18)F-BCPP-EF binding (V T) and (11)C-PIB uptake (SUVR), and there was a significant positive correlation between (11)C-DPA-713 uptake (SUV) and (11)C-PIB uptake. In contrast, there was no significant correlation between rCMRglc ratio and (11)C-PIB uptake.
  
  
  CONCLUSIONS
  (18)F-BCPP-EF could be a potential PET probe for quantitative imaging of impaired MC-I activity that is correlated with Aβ deposition in the living brain.
authors.affiliation: Central Research Laboratory, Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamakita, Shizuoka, 434-8601, Japan, tsukada@crl.hpk.co.jp.
authors.forename: Hideo
authors.initials: H
authors.lastname: Tsukada
authors.forename: Shingo
authors.initials: S
authors.lastname: Nishiyama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohba
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Kanazawa
authors.forename: Takeharu
authors.initials: T
authors.lastname: Kakiuchi
authors.forename: Norihiro
authors.initials: N
authors.lastname: Harada
mesh_terms: D000375:Aging / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism*; D042967:Electron Transport Complex I / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007249:Inflammation / Q000000981:diagnostic imaging / Q000378:metabolism; D008253:Macaca mulatta; D008297:Male; D049268:Positron-Emission Tomography; D011724:Pyridazines; D011725:Pyridines
chemical_list: C588084:2-tert-butyl-4-chloro-5-(6-(2-(2-fluoroethoxy)ethoxy)pyridin-3-ylmethoxy)-2H-pyridazin-3-one; D016229:Amyloid beta-Peptides; D011724:Pyridazines; D011725:Pyridines; D042967:Electron Transport Complex I; D005947:Glucose