title: Comparison of the effects of dexmedetomidine administered at two different times on renal ischemia/reperfusion injury in rats.
abstract: OBJECTIVE
  We investigated the effect of dexmedetomidine on ischemic renal failure in rats.
  
  
  METHODS
  In the present study, 26 male adult Wistar albino rats weighting 230-300 g were randomly separated into four groups: sham-operated (n=5), ischemia reperfusion (IR) (IR group, n=7), IR/reperfusion treatment with dexmedetomidine (Dex. R group, n=7) and IR/pre-ischemic treatment with dexmedetomidine (Dex. I group, n=7). In the first group, sham operation was achieved and renal clamps were not applied. For the IR group, renal ischemia was induced by occlusion of the bilateral renal arteries and veins for 60 min followed by reperfusion for 24h. For the Dex. R and Dex. I groups, the same surgical procedure as in the IR group was performed, and dexmedetomidine (100 mcg/kg intraperitoneal) was administrated at the 5th min after reperfusion and before ischemia. At the end of reperfusion, blood samples were drawn, the rats were sacrificed, and the left kidney was processed for histopathology.
  
  
  RESULTS
  The blood urea nitrogen (BUN) levels in groups Dex. R and Dex. I were significantly lower than in the IR group (p=0.015, p=0.043), although urine flow was significantly higher in group Dex. R (p=0.003). The renal histopathological score in the IR group was significantly higher than in the other groups. There was no significant difference between the Dex. R and Dex. I groups.
  
  
  CONCLUSIONS
  The results were shown that administration of dexmedetomidine reduced the renal IR injury histomorphologically. Administration of dexmedetomidine in the reperfusion period was considered as more effective due to increase in urinary output and decrease in BUN levels.
authors.affiliation: Department of Anesthesiology, Van Training and Research Hospital, Van, Turkey. Electronic address: edipgonullu@gmail.com.
authors.forename: Edip
authors.initials: E
authors.lastname: Gonullu
authors.affiliation: Department of Anesthesiology, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Sevda
authors.initials: S
authors.lastname: Ozkardesler
authors.affiliation: Department of Biochemistry, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Tuncay
authors.initials: T
authors.lastname: Kume
authors.affiliation: Department of Anesthesiology, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Leyla Seden
authors.initials: LS
authors.lastname: Duru
authors.affiliation: Department of Anesthesiology, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Mert
authors.initials: M
authors.lastname: Akan
authors.affiliation: Department of Laboratory Animal, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Mehmet Ensari
authors.initials: ME
authors.lastname: Guneli
authors.affiliation: Department of Histology and Embryology, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Bekir Ugur
authors.initials: BU
authors.lastname: Ergur
authors.affiliation: Department of Nutrition and Dietetics, College of Health Sciences, Ege University, Izmir, Turkey.
authors.forename: Reci
authors.initials: R
authors.lastname: Meseri
authors.affiliation: Department of Anesthesiology, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Oytun
authors.initials: O
authors.lastname: Dora
mesh_terms: D058186:Acute Kidney Injury / Q000503:physiopathology / Q000517:prevention & control*; D058647:Adrenergic alpha-2 Receptor Agonists / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001806:Blood Urea Nitrogen; D020927:Dexmedetomidine / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D058647:Adrenergic alpha-2 Receptor Agonists; D020927:Dexmedetomidine
keywords: Acute renal failure; Dexmedetomidine; Ischemia/reperfusion; Kidney