title: Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread.
abstract: Tobacco mosaic virus (TMV) is a longstanding model for studying virus movement and macromolecular transport through plasmodesmata (PD). Its movement protein (MP) interacts with cortical microtubule (MT)-associated ER sites (C-MERs) to facilitate the formation and transport of ER-associated viral replication complexes (VRCs) along the ER-actin network towards PD. To investigate whether this movement mechanism might be conserved between tobamoviruses, we compared the functions of Oilseed rape mosaic virus (ORMV) MP with those of MP(TMV). We show that MP(ORMV) supports TMV movement more efficiently than MP(TMV). Moreover, MP(ORMV) localizes to C-MERs like MP(TMV) but accumulates to lower levels and does not localize to larger inclusions/VRCs or along MTs, patterns regularly seen for MP(TMV). Our findings extend the role of C-MERs in viral cell-to-cell transport to a virus commonly used for functional genomics in Arabidopsis. Moreover, accumulation of tobamoviral MP in inclusions or along MTs is not required for virus movement.
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS (UPR 2357), Université de Strasbourg, 12 rue du Général Zimmer, 67000 Strasbourg, France; Zürich-Basel Plant Science Center, Department of Environmental Sciences, Botany, University of Basel, Hebelstrasse 1, 4056 Basel, Switzerland. Electronic address: annette.niehl@unibas.ch.
authors.forename: Annette
authors.initials: A
authors.lastname: Niehl
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS (UPR 2357), Université de Strasbourg, 12 rue du Général Zimmer, 67000 Strasbourg, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Pasquier
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Ferriol
authors.affiliation: Laboratoire de Biophotonique et Pharmacologie, CNRS (UMR 7213), Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Mély
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS (UPR 2357), Université de Strasbourg, 12 rue du Général Zimmer, 67000 Strasbourg, France; Zürich-Basel Plant Science Center, Department of Environmental Sciences, Botany, University of Basel, Hebelstrasse 1, 4056 Basel, Switzerland. Electronic address: manfred.heinlein@ibmp-cnrs.unistra.fr.
authors.forename: Manfred
authors.initials: M
authors.lastname: Heinlein
mesh_terms: D017360:Arabidopsis / Q000821:virology*; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000821:virology; D007181:Inclusion Bodies, Viral; D008870:Microtubules / Q000378:metabolism; D053830:Plant Viral Movement Proteins / Q000235:genetics / Q000378:metabolism*; D017898:Tobamovirus / Q000235:genetics / Q000502:physiology*
chemical_list: D053830:Plant Viral Movement Proteins
keywords: Cell-to-cell movement; Endoplasmic reticulum; Microtubules; Movement protein; Tobamovirus; Viral replication complex