title: Recovery of lead-induced suppressed reproduction in male rats by testosterone.
abstract: The objective of the present study was to investigate the effects of testosterone in recuperation of lead-induced suppressed reproduction in adult male rats. Lead acetate was administered orally to adult male rats (95 ± 5 days) at dosage level of 0.05 and 0.15% for 55 days through drinking water and injected intraperitoneally with either testoviron depot at a dose of 4.16 mg kg(-1) body weight or vehicle alone on days 1, 7 and 14 respectively. At the end of treatment, control and treated males were cohabited with untreated normal-cycling females. After cohabitation for 5 days, all the male rats were killed and weights of reproductive organs were determined. Significant increase in the indices of testis, epididymis, seminal vesicles, vas deferens and prostate glands was observed in testosterone (T)-treated rats when compared to those of lead-exposed rats. Testosterone treatment significantly increased epididymal sperm count, motile spermatozoa, viable spermatozoa and HOS tail-coiled spermatozoa and also the activity levels of testicular 3β- and 17β-hydroxysteroid dehydrogenases when compared to those of lead-exposed males. From the results, it can be hypothesised that supplementation of testosterone mitigated lead-induced suppressed reproduction in male rats.
authors.affiliation: Department of Biotechnology, S.V.University, Tirupati, India.
authors.forename: M
authors.initials: M
authors.lastname: Reshma Anjum
authors.forename: P
authors.initials: P
authors.lastname: Sreenivasula Reddy
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000187:drug effects / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000187:drug effects / Q000378:metabolism; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D004822:Epididymis / Q000187:drug effects / Q000473:pathology; D005640:Follicle Stimulating Hormone / Q000097:blood; D007248:Infertility, Male / Q000139:chemically induced*; D007986:Luteinizing Hormone / Q000097:blood / Q000187:drug effects; D008297:Male; D009929:Organ Size; D009942:Organometallic Compounds / Q000633:toxicity*; D011467:Prostate / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012669:Seminal Vesicles / Q000187:drug effects / Q000473:pathology; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000494:pharmacology*; D014649:Vas Deferens / Q000187:drug effects / Q000473:pathology
chemical_list: D000728:Androgens; D009942:Organometallic Compounds; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases; C008261:lead acetate
keywords: Lead acetate; rat; spermatozoa; testis; testosterone