title: Crystallization and preliminary X-ray characterization of the 2,4'-dihydroxyacetophenone dioxygenase from Alcaligenes sp. 4HAP.
abstract: The enzyme 2,4'-dihydroxyacetophenone dioxygenase (or DAD) catalyses the conversion of 2,4'-dihydroxyacetophenone to 4-hydroxybenzoic acid and formic acid with the incorporation of molecular oxygen. Whilst the vast majority of dioxygenases cleave within the aromatic ring of the substrate, DAD is very unusual in that it is involved in C-C bond cleavage in a substituent of the aromatic ring. There is evidence that the enzyme is a homotetramer of 20.3 kDa subunits each containing nonhaem iron and its sequence suggests that it belongs to the cupin family of dioxygenases. By the use of limited chymotrypsinolysis, the DAD enzyme from Alcaligenes sp. 4HAP has been crystallized in a form that diffracts synchrotron radiation to a resolution of 2.2 Å.
authors.affiliation: School of Biological Sciences, University of Southampton, Southampton, England.
authors.forename: G
authors.initials: G
authors.lastname: Beaven
authors.affiliation: School of Biological Sciences, University of Southampton, Southampton, England.
authors.forename: A
authors.initials: A
authors.lastname: Bowyer
authors.affiliation: Laboratory of Protein Crystallography, Centre for Amyloidosis and Acute Phase Proteins, UCL Division of Medicine (Royal Free Campus), Rowland Hill Street, London NW3 2PF, England.
authors.forename: P
authors.initials: P
authors.lastname: Erskine
authors.affiliation: Laboratory of Protein Crystallography, Centre for Amyloidosis and Acute Phase Proteins, UCL Division of Medicine (Royal Free Campus), Rowland Hill Street, London NW3 2PF, England.
authors.forename: S P
authors.initials: SP
authors.lastname: Wood
authors.affiliation: Cambridge Institute for Medical Research, University of Cambridge, Cambridge, England.
authors.forename: A
authors.initials: A
authors.lastname: McCoy
authors.affiliation: Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
authors.forename: L
authors.initials: L
authors.lastname: Coates
authors.affiliation: STFC Rutherford Appleton Laboratory, RAL, Harwell, Oxford, Didcot OX11 0FA, England.
authors.forename: R
authors.initials: R
authors.lastname: Keegan
authors.affiliation: STFC Rutherford Appleton Laboratory, RAL, Harwell, Oxford, Didcot OX11 0FA, England.
authors.forename: A
authors.initials: A
authors.lastname: Lebedev
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Penglais, Aberystwyth SY23 3DA, Wales.
authors.forename: D J
authors.initials: DJ
authors.lastname: Hopper
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Penglais, Aberystwyth SY23 3DA, Wales.
authors.forename: M A
authors.initials: MA
authors.lastname: Kaderbhai
authors.affiliation: Laboratory of Protein Crystallography, Centre for Amyloidosis and Acute Phase Proteins, UCL Division of Medicine (Royal Free Campus), Rowland Hill Street, London NW3 2PF, England.
authors.forename: J B
authors.initials: JB
authors.lastname: Cooper
mesh_terms: D000421:Alcaligenes / Q000201:enzymology*; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D017931:DNA Primers; D049308:Dioxygenases / Q000737:chemistry*; D006868:Hydrolysis; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D049308:Dioxygenases; C403717:2,4'-dihydroxyacetophenone dioxygenase
keywords: 2,4′-dihydroxyacetophenone dioxygenase; Alcaligenes sp. 4HAP; limited proteolysis