title: An optimized HPLC method for soil fungal biomass determination and its application to a detritus manipulation study.
abstract: The goal of this research was to develop modified analytical method for the quantitative analysis of ergosterol, which is highly effective marker for fungal biomass. We suggest that our optimized method for the determination of ergosterol is an effective way to monitor changes in fungal biomass under different environmental conditions.
authors.affiliation: Institute of Environmental Sciences, College of Nyiregyhaza, Sóstói u 31/B, Nyiregyhaza, Hungary. Electronic address: beniaron@nyf.hu.
authors.forename: Aron
authors.initials: A
authors.lastname: Beni
authors.affiliation: Institute of Environmental Sciences, College of Nyiregyhaza, Sóstói u 31/B, Nyiregyhaza, Hungary.
authors.forename: Erzsebet
authors.initials: E
authors.lastname: Soki
authors.affiliation: Oregon State University, Department of Crop and Soil Science, 3017 Agricultural and Life Sciences Building, Corvallis, OR 97331, USA.
authors.forename: Kate
authors.initials: K
authors.lastname: Lajtha
authors.affiliation: Institute of Environmental Sciences, College of Nyiregyhaza, Sóstói u 31/B, Nyiregyhaza, Hungary.
authors.forename: Istvan
authors.initials: I
authors.lastname: Fekete
mesh_terms: D018533:Biomass; D002851:Chromatography, High Pressure Liquid; D004777:Environment; D004875:Ergosterol / Q000032:analysis / Q000737:chemistry; D065928:Forests; D005658:Fungi / Q000737:chemistry*; D015203:Reproducibility of Results; D012988:Soil Microbiology
chemical_list: D004875:Ergosterol
keywords: DIRT; Ergosterol; Fungal biomass; HPLC; Litter manipulation; SPE