title: Preparation and chromatographic evaluation of new branch-type diamide-embedded octadecyl stationary phase with enhanced shape selectivity.
abstract: A novel branch-type diamide-embedded octadecyl stationary phase was prepared by facile amidation. The preparation of this new phase involves the synthesis of new bifunctional silane ligand and surface modification of spherical silica via anchoring of silane coupling agent. The obtained diamide-embedded octadecyl stationary phase demonstrated excellent hydrophobic selectivity, as well as enhanced shape and planarity selectivity in comparison to commercial polymeric and monomeric C18 phases, respectively, as revealed by the systematic investigation into its liquid chromatographic retention of isomeric polycyclic aromatic hydrocarbons. The applicability of this new stationary phase was further testified by the effective separation of isomeric compounds belong to different chemical classes, including chain isomers of alkylbenzenes, and positional isomers of substituted aromatics. An in-depth analysis of the separation mechanisms other than molecular shape recognition involved in the new stationary phase was performed using a linear solvation energy relationships model and compared with its monoamide and pure C18 counterparts correspondingly. The performance of the new stationary phase in quantitative analysis of phenols from real-world samples was also evaluated.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Applied Chemistry and Biochemistry, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
authors.forename: Abul K
authors.initials: AK
authors.lastname: Mallik
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China. Electronic address: hdqiu@licp.cas.cn.
authors.forename: Hongdeng
authors.initials: H
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China. Electronic address: sxjiang@lzb.ac.cn.
authors.forename: Shengxiang
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Applied Chemistry and Biochemistry, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Ihara
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D003958:Diamide / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007536:Isomerism; D010636:Phenols / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis
chemical_list: D010636:Phenols; D011084:Polycyclic Aromatic Hydrocarbons; D003958:Diamide
keywords: Amide-embedded; Octadecyl phase; Shape and planarity selectivity; Structural isomers