title: Characterisation and preliminary lipid-lowering evaluation of Lactobacillus isolated from a traditional Serbian dairy product.
abstract: We investigated the potential probiotic properties of indigenous lactic acid bacteria (LAB) isolated from Serbian homemade cheese. Seventeen LAB strains were isolated and characterised using standard protocols. One of the strains showed several probiotic properties: survival at low pH and in bile salts solution, antimicrobial activity, susceptibility to antibiotics and adhesion to hexodecane. DNA analysis identified the isolate as Lactobacillus casei, hereafter named L. casei 5s. The lipid lowering effect of L. casei 5s was evaluated in vivo using a hyperlipidemic rat model. Orally administered L. casei 5s significantly decreased the elevated total serum cholesterol and triglycerides, and attenuated macro vesicular steatosis in the liver. Moreover, L. casei 5s improved the intestinal microbial balance in favour of lactobacilli, while decreasing the number of Escherichia coli cells. The bacteria were re-isolated and identified from the surface of the intestinal mucosa and from the faecal samples of treated animals, indicating adhesiveness and colonisation ability. The results of an acute oral toxicity study in mice and the absence of translocation to other organs demonstrated the safety of the strain. In conclusion, L. casei 5s demonstrated promising probiotic potential and might be a good candidate for more detailed investigations.
authors.affiliation: Galenika a.d., R×D Institute, Batajnièki drum bb, 11080 Belgrade, Serbia.
authors.forename: G
authors.initials: G
authors.lastname: Zavišić
authors.affiliation: Galenika a.d., R×D Institute, Batajnièki drum bb, 11080 Belgrade, Serbia.
authors.forename: S
authors.initials: S
authors.lastname: Ristić
authors.affiliation: Galenika a.d., R×D Institute, Batajnièki drum bb, 11080 Belgrade, Serbia.
authors.forename: S
authors.initials: S
authors.lastname: Petrièević
authors.affiliation: Galenika a.d., R×D Institute, Batajnièki drum bb, 11080 Belgrade, Serbia.
authors.forename: J
authors.initials: J
authors.lastname: Novaković Jovanović
authors.affiliation: Galenika a.d., R×D Institute, Batajnièki drum bb, 11080 Belgrade, Serbia.
authors.forename: Ž
authors.initials: Ž
authors.lastname: Radulović
authors.affiliation: Institute for Biological Research 'Siniša Stanković', University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: B
authors.initials: B
authors.lastname: Janać Petković
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010 Belgrade, Serbia.
authors.forename: I
authors.initials: I
authors.lastname: Strahinić
authors.affiliation: Galenika a.d., R×D Institute, Batajnièki drum bb, 11080 Belgrade, Serbia.
authors.forename: V
authors.initials: V
authors.lastname: Piperski
mesh_terms: D000284:Administration, Oral; D000818:Animals; D058491:Bacterial Load; D058448:Biota; D003611:Dairy Products / Q000382:microbiology*; D004032:Diet / Q000379:methods; D004926:Escherichia coli / Q000302:isolation & purification; D005223:Fats / Q000008:administration & dosage*; D005234:Fatty Liver / Q000473:pathology / Q000517:prevention & control; D041981:Gastrointestinal Tract / Q000382:microbiology; D006949:Hyperlipidemias / Q000517:prevention & control; D007780:Lacticaseibacillus casei / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008055:Lipids / Q000097:blood*; D019936:Probiotics / Q000008:administration & dosage*; D051381:Rats; D016896:Treatment Outcome
chemical_list: D005223:Fats; D008055:Lipids
keywords: Lactobacillus casei; lipid-lowering effect; probiotic; safety