title: Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus.
abstract: 2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroG(fbr) from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3g/L of 2-PE was produced from 20g/L glucose without addition of phenylalanine in the medium.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Tae-Yeon
authors.initials: TY
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea. Electronic address: mkoh@korea.ac.kr.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Oh
mesh_terms: D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000235:genetics / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D019020:Directed Molecular Evolution; D005800:Genes, Fungal; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism*; D007716:Kluyveromyces / Q000235:genetics* / Q000378:metabolism*; D060847:Metabolic Engineering; D010626:Phenylethyl Alcohol / Q000378:metabolism*; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C509911:ADH2 protein, S cerevisiae; D000426:Alcohol Dehydrogenase; D010723:3-Deoxy-7-Phosphoheptulonate Synthase; D002262:Carboxy-Lyases; C109058:phenylpyruvate decarboxylase; D005947:Glucose; D010626:Phenylethyl Alcohol
keywords: 2-Phenylethanol; DHAP synthase; Ehrlich pathway; Kluyveromyces marxianus