title: Human breast adipose‑derived stem cells: characterization and differentiation into mammary gland‑like epithelial cells promoted by autologous activated platelet‑rich plasma.
abstract: Human adipose‑derived stem cells (ASCs) isolated from various body sites have been widely investigated in basic and clinical studies. However, ASCs derived from human breast tissue (hbASCs) have not been extensively investigated. In order to expand our understanding of hbASCs and examine their potential applications in stem cell research and cell‑based therapy, hbASCs were isolated from discarded surgical fat tissue following reduction mammoplasty and a comprehensive characterization of these hbASCs was performed, including analysis of their cellular morphology, growth features, cell surface protein markers and multilineage differentiation capacity. These hbASCs expressed cluster of differentiation (CD)44, CD49d, CD90 and CD105, but did not express CD31 and CD34. Subsequently, the hbASCs were differentiated into adipocytes, osteocytes and chondrocytes in vitro. In order to examine the potential applications of hbASCs in breast reconstruction, an approach to promote in vitro differentiation of hbASCs into mammary gland‑like epithelial cells (MGECs) was developed using activated autologous platelet‑rich plasma (PRP). A proliferation phase and a subsequent morphological conversion phase were observed during this differentiation process. PRP significantly promoted the growth of hbASCs in the proliferation phase and increased the eventual conversion rate of hbASCs into MGECs. Thus, to the best of our knowledge, the present study provided the first comprehensive characterization of hbASCs and validated their multipotency. Furthermore, it was revealed that activated autologous PRP was able to enhance the differentiation efficiency of hbASCs into MGECs. The present study and other studies of hbASCs may aid the development of improved breast reconstruction strategies.
authors.affiliation: Department of Mammary Gland Surgery, Zhongshan Hospital of Sun Yat‑Sen University, Zhongshan, Guangdong 528403, P.R. China.
authors.forename: Shi-En
authors.initials: SE
authors.lastname: Cui
authors.affiliation: Department of Plastic and Aesthetic Surgery, Zhongshan Bo'ai Hospital of Southern Medical University, Zhongshan, Guangdong 528403, P.R. China.
authors.forename: Hong-Mian
authors.initials: HM
authors.lastname: Li
authors.affiliation: Department of Plastic and Reconstructive Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.
authors.forename: Da-Lie
authors.initials: DL
authors.lastname: Liu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Nan
authors.affiliation: Department of Plastic and Aesthetic Surgery, Zhongshan Bo'ai Hospital of Southern Medical University, Zhongshan, Guangdong 528403, P.R. China.
authors.forename: Kun-Ming
authors.initials: KM
authors.lastname: Xu
authors.affiliation: Research Center for Tissue Engineering, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Pei-Ran
authors.initials: PR
authors.lastname: Zhao
authors.affiliation: Research Center for Tissue Engineering, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Shuang-Wu
authors.initials: SW
authors.lastname: Liang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D001940:Breast / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D042361:Mammary Glands, Human / Q000166:cytology; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D003470:Culture Media