title: Ferruginibacter profundus sp. nov., a novel member of the family Chitinophagaceae, isolated from freshwater sediment of a reservoir.
abstract: A Gram-negative, aerobic, non-motile, and rod-shaped bacterium, designated strain DS48-5-3(T), was isolated from a 48 m sediment sample taken from Daechung Reservoir, Republic of Korea. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this strain to the Bacteroidetes, notably most closely related to Ferruginibacter alkalilentus HU1-GD23(T), Ferruginibacter lapsinanis HU1-HG42(T) and Ferruginibacter yonginensis HME8442(T), showing 16S rRNA gene sequence similarities to the type strains of these species of 95.2-96.4 % similarity. The predominant ubiquinone was identified as MK-7. The major fatty acids were identified as iso-C15:0, iso-C17:0 3-OH, and iso-C15:1 G. The G+C content of the genomic DNA of strain DS48-5-3(T) was determined to be 37.2 %. On the basis of polyphasic evidence, it is proposed that strain DS48-5-3(T) should belong to a novel species, for which the name Ferruginibacter profundus sp. nov. (type strain DS48-5-3(T) = KCTC 32478(T) = JCM 19431(T)), is proposed.
authors.affiliation: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.forename: Hyung-Gwan
authors.initials: HG
authors.lastname: Lee
authors.forename: Hyun-Joon
authors.initials: HJ
authors.lastname: La
authors.forename: So-Ra
authors.initials: SR
authors.lastname: Ko
authors.forename: Chi-Yong
authors.initials: CY
authors.lastname: Ahn
authors.forename: Hee-Mock
authors.initials: HM
authors.lastname: Oh
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology*; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S