title: Carnitine/acylcarnitine translocase and carnitine palmitoyltransferase 2 form a complex in the inner mitochondrial membrane.
abstract: Carnitine/acylcarnitine translocase and carnitine palmitoyltransferase 2 are members of the carnitine system, which are responsible of the regulation of the mitochondrial CoA/acyl-CoA ratio and of supplying substrates for the ß-oxidation to mitochondria. This study, using cross-Linking reagent, Blue native electrophoresis and immunoprecipitation followed by detection with immunoblotting, shows conclusive evidence about the interaction between carnitine palmitoyltransferase 2 and carnitine/acylcarnitine translocase supporting the channeling of acylcarnitines and carnitine at level of the inner mitochondrial membrane.
authors.affiliation: Department BEST (Biologia, Ecologia, Scienze della Terra), Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Arcavacata di Rende, Cosenza, Italy.
authors.forename: Lara
authors.initials: L
authors.lastname: Console
authors.forename: Nicola
authors.initials: N
authors.lastname: Giangregorio
authors.forename: Cesare
authors.initials: C
authors.lastname: Indiveri
authors.forename: Annamaria
authors.initials: A
authors.lastname: Tonazzi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002331:Carnitine / Q000031:analogs & derivatives / Q000378:metabolism; D002333:Carnitine Acyltransferases / Q000378:metabolism*; D002334:Carnitine O-Palmitoyltransferase / Q000737:chemistry / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry; D047468:Immunoprecipitation; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000201:enzymology*; D051336:Mitochondrial Membranes / Q000201:enzymology*; D008958:Models, Molecular; D063347:Native Polyacrylamide Gel Electrophoresis; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats
chemical_list: D003432:Cross-Linking Reagents; D026901:Membrane Transport Proteins; C116917:acylcarnitine; D005557:Formaldehyde; D002333:Carnitine Acyltransferases; C000602144:Slc25a20 protein, rat; D002334:Carnitine O-Palmitoyltransferase; D002331:Carnitine