title: Plasmid-based Stat3-specific siRNA and GRIM-19 inhibit the growth of thyroid cancer cells in vitro and in vivo.
abstract: It has been shown that overexpression of signal transducer and activator of transcription 3 (Stat3) contribute to the progression and metastasis of various solid tumors and that silencing Stat3 inhibits tumor growth in several types of cancer. Gene associated with retinoid-IFN-induced mortality 19 (GRIM-19), a Stat3-inhibitory protein, was identified as a potential tumor suppressor associated with growth inhibition and cell apoptosis by targeting the transcription factor Stat3 for inhibition. However, little is known about Stat3 and GRIM-19 roles in the tumor growth of thyroid carcinoma cells. In the present study, we developed a dual expression plasmid that co-expressed Stat3-specific siRNA and GRIM-19 (pSi-Stat3-GRIM-19) and transfected it into SW579 cells (thyroid carcinoma cell line) to evaluate its effects on cell proliferation, cell apoptosis, cell migration and cell invasion in vitro and tumor growth in vivo. Simultaneous expression of pSi-Stat3-GRIM-19 in SW579 cancer cells was found to significantly suppress the proliferation, migration and invasion in vitro and tumor growth in vivo, when compared to the controls either Stat3-specific siRNA or GRIM-19 alone. In conclusion, our data demonstrated that a combined strategy of co-expressed Stat3-specific siRNA and GRIM19 synergistically and more effectively suppressed thyroid tumor growth, and have therapeutic potential for the treatment of thyroid cancer.
authors.affiliation: Department of Thyroid Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Gui-Min
authors.initials: GM
authors.lastname: Wang
authors.affiliation: Department of Pathophysiology, College of Basic Medical Sciences of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Zhong-Xi
authors.initials: ZX
authors.lastname: Ren
authors.affiliation: Department of Thyroid Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Pei-Song
authors.initials: PS
authors.lastname: Wang
authors.affiliation: Department of Thyroid Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Chang
authors.initials: C
authors.lastname: Su
authors.affiliation: Department of Thyroid Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Wen-Xin
authors.initials: WX
authors.lastname: Zhang
authors.affiliation: Department of Thyroid Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Zeng-Guang
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China.
authors.forename: Xue-Jian
authors.initials: XJ
authors.lastname: Zhao
authors.affiliation: Department of Thyroid Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D034741:RNA, Small Interfering / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors* / Q000235:genetics; D013964:Thyroid Neoplasms / Q000473:pathology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D051017:Apoptosis Regulatory Proteins; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; D009247:NADH, NADPH Oxidoreductases; C439057:NDUFA13 protein, human