title: HLA antigen distribution in renal transplant recipients and donors.
abstract: BACKGROUND
  HLA typing analysis is important in renal transplant patient. This study is the first report from Nepal to find out distribution of HLA A, B, DR antigen in live related renal transplant recipients and donors from Nepal. The aim of this study was to investigate the distribution of HLA in the live related renal transplant recipients and donors of Nepal.
  
  
  METHODS
  In a retrospective study, HLA patterns were defined in 100 live related renal transplant recipients and donors. One year study is done from June 2011 to May 2012. The study was done by using sequence specific oligonucleotides primers and polymerase chain reaction and assay. Allele frequencies were obtained by direct counting.
  
  
  RESULTS
  A total of 12 HLA-A, 15 HLA-B and 13 HLA-DRB1 alleles were identified at the four-digit level in the live related renal transplant recipients and donors of Nepal. High frequency alleles were HLA-A*11 (34.5%), A*24 (17%), A*33 (13%); HLA-B*15(27%), B*35(19%), B*40 (10%); HLA-DRB1*15 (33.5%), DRB1*12 (21.4%) and DRB1*04 (7.32%).
  
  
  CONCLUSIONS
  These results showed considerable heterogeneity in both HLA class I and class II antigens. To reduce the risk of allograft rejection, transplantation from HLA matched donors is recommended.
authors.affiliation: Department of Pathology, Aarogya Foundation, Pulchowk, Lalitapur, Nepal.
authors.forename: A
authors.initials: A
authors.lastname: Tuladhar
authors.affiliation: Department of Pathology, Aarogya Foundation, Pulchowk, Lalitapur, Nepal.
authors.forename: S
authors.initials: S
authors.lastname: Shrestha
authors.affiliation: Department of Pathology, Aarogya Foundation, Pulchowk, Lalitapur, Nepal.
authors.forename: P P
authors.initials: PP
authors.lastname: Raut
authors.affiliation: Department of Pathology, Aarogya Foundation, Pulchowk, Lalitapur, Nepal.
authors.forename: P
authors.initials: P
authors.lastname: Bhandari
authors.affiliation: Department of Nephrology, National Academy of Medical Sciences, Kathmandu, Nepal.
authors.forename: P
authors.initials: P
authors.lastname: Shrestha
mesh_terms: D005190:Family; D005260:Female; D005787:Gene Frequency; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D009390:Nepal; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D014019:Tissue Donors; D066027:Transplant Recipients
chemical_list: D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens