title: Protein expression changes during cotton fiber elongation in response to drought stress and recovery.
abstract: An investigation to better understand the molecular mechanism of cotton (Gossypium hirsutum L.) fiber elongation in response to drought stress and recovery was conducted using a comparative proteomics analysis. Cotton plants (cv. NuCOTN 33B) were subjected to water deprivation for 10 days followed by a recovery period (with watering) of 5 days. The temporal changes in total proteins in cotton fibers were examined using 2DE. The results revealed that 163 proteins are significantly drought responsive. MS analysis led to the identification of 132 differentially expressed proteins that include some known as well as some novel drought-responsive proteins. These drought responsive fiber proteins in NuCOTN 33B are associated with a variety of cellular functions, i.e. signal transduction, protein processing, redox homeostasis, cell wall modification, metabolisms of carbon, energy, lipid, lignin, and flavonoid. The results suggest that the enhancement of the perception of drought stress, a new balance of the metabolism of the biosynthesis of cell wall components and cytoskeleton homeostasis plays an important role in the response of cotton fibers to drought stress. Overall, the current study provides an overview of the molecular mechanism of drought response in cotton fiber cells.
authors.affiliation: College of Agriculture, Nanjing Agricultural University, Nanjing, P. R. China; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, P. R. China.
authors.forename: Mi
authors.initials: M
authors.lastname: Zheng
authors.forename: Yali
authors.initials: Y
authors.lastname: Meng
authors.forename: Changqin
authors.initials: C
authors.lastname: Yang
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhou
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
authors.forename: Binglin
authors.initials: B
authors.lastname: Chen
mesh_terms: D016000:Cluster Analysis; D032561:Cotton Fiber; D055864:Droughts; D015180:Electrophoresis, Gel, Two-Dimensional; D003368:Gossypium / Q000378:metabolism* / Q000502:physiology*; D008954:Models, Biological; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D040901:Proteomics; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Cotton; Drought; Dynamic changes; Fiber elongation; Plant proteomics