title: Whole genome sequence of Clostridium bornimense strain M2/40 isolated from a lab-scale mesophilic two-phase biogas reactor digesting maize silage and wheat straw.
abstract: The bacterium Clostridium bornimense M2/40 is a mesophilic, anaerobic bacterium isolated from a two-phase biogas reactor continuously fed with maize silage and 5% wheat straw. Grown on glucose, it produced H2, CO2, formiate, lactate and propionate as the main fermentation products, of which some compounds serve as substrates for methanogenic Archaea to form methane. Here, the whole genome sequence of the bacterium consisting of two circular replicons is reported. This genome information provides the basis for further studies addressing metabolic features of the isolate and its role in anaerobic biomass degradation.
authors.affiliation: Department of Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Max-Eyth-Allee 100, 14469 Potsdam, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Hahnke
authors.affiliation: Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Universitätsstr. 27, 33615 Bielefeld, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wibberg
authors.affiliation: Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Universitätsstr. 27, 33615 Bielefeld, Germany.
authors.forename: Geizecler
authors.initials: G
authors.lastname: Tomazetto
authors.affiliation: Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Universitätsstr. 27, 33615 Bielefeld, Germany.
authors.forename: Alfred
authors.initials: A
authors.lastname: Pühler
authors.affiliation: Department of Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Max-Eyth-Allee 100, 14469 Potsdam, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Klocke
authors.affiliation: Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Universitätsstr. 27, 33615 Bielefeld, Germany. Electronic address: aschluet@CeBiTec.Uni-Bielefeld.DE.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schlüter
mesh_terms: D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology; D003013:Clostridium / Q000235:genetics*; D005285:Fermentation; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D008697:Methane / Q000378:metabolism; D008969:Molecular Sequence Data; D012820:Silage / Q000382:microbiology; D014908:Triticum / Q000737:chemistry / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism
chemical_list: D056804:Biofuels; D008697:Methane
keywords: Anaerobic digestion; Biomass degradation; Biomethanation; Chromid; Clostridium