title: Effect of simulated intraoral erosion and/or abrasion effects on etch-and-rinse bonding to enamel.
abstract: OBJECTIVE
  To assess the influence of simulated oral erosive/abrasive challenges on the bond strength of an etch-and-rinse two-step bonding system to enamel using an in situ/ex vivo protocol.
  
  
  METHODS
  Bovine enamel blocks were prepared and randomly assigned to four groups: CONT - control (no challenge), ABR - 3x/day-1 minute toothbrushing; ERO - 3x/day - 5 minutes extraoral immersion into regular Coca Cola; and ERO+ABR - erosive protocol followed by a 1-minute toothbrushing. Eight blocks were placed into an acrylic palatal appliance for each volunteer (n = 13), who wore the appliance for 5 days. Two blocks were subjected to each of the four challenges. Subsequently, all the blocks were washed with tap water and Adper Single Bond 2/Filtek Z350 were placed. After 24 hours, 1 mm2 beams were obtained from each block to be tested with the microtensile bond strength test (50 N load at 0.5 mm/minute). The data were statistically analyzed by one-way RM-ANOVA and Tukey's tests (alpha = 0.05).
  
  
  RESULTS
  No difference was detected among the ABR, ERO, and CONT groups (P > 0.05). ERO+ABR group yielded lower bond strengths than either the ABR and ERO groups (P < 0.0113).
authors.forename: Linda
authors.initials: L
authors.lastname: Wang
authors.forename: Leslie C
authors.initials: LC
authors.lastname: Casas-Apayco
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Hipólito
authors.forename: Vanessa Manzini
authors.initials: VM
authors.lastname: Dreibi
authors.forename: Marina Ciccone
authors.initials: MC
authors.lastname: Giacomini
authors.forename: Odair
authors.initials: O
authors.lastname: Bim Júnior
authors.forename: Daniela
authors.initials: D
authors.lastname: Rios
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Magalhães
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002253:Carbonated Beverages / Q000009:adverse effects; D002417:Cattle; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D003743:Dental Enamel / Q000648:ultrastructure*; D003764:Dental Materials / Q000737:chemistry; D003799:Dental Stress Analysis / Q000295:instrumentation; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010756:Phosphoric Acids / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors; D014072:Tooth Abrasion / Q000473:pathology*; D014077:Tooth Erosion / Q000473:pathology*; D014099:Toothbrushing / Q000009:adverse effects
chemical_list: D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; D010756:Phosphoric Acids; C533204:filtek Z350; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; C030242:phosphoric acid