title: Effects of fructus psoraleae extract on the intestinal absorption kinetics of geniposide and geniposidic acid in rat.
abstract: Cortex Eucommia has been used as a kidney-tonifying herbal medicine with a long history of compatibility with Fructus Psoraleae. Geniposide (GP) and geniposidic acid (GPA) are the two main chemical components in Cortex Eucommia. In the present study, the effects of Fructus Psoraleae extract (FPE) on intestinal absorption kinetics of GP and GPA in rat were investigated. Twenty four male Sprague-Dawley rats were randomly assigned into four groups which were treated with GP, GPA, GP mixed with FPE and GPA mixed with FPE, respectively, by in situ intestinal perfusion for 3 h. The samples of intestinal perfusion solutions were collected every 30 min, and analyzed by ultra high performance liquid chromatography (UPLC). The curves of time and residual quantities of GP and GPA (lnx) in the intestinal perfusion solution and the cumulative absorption rate were obtained. The results showed that FPE exhibited different effects on the intestinal absorption of GP and GPA in rat: it increased the intestinal absorption of GP (p<0.05), while demonstrated no significant effect on the absorption of GPA.
authors.affiliation: Tianjin University of Traditional Chinese Medicine, 312 Yuquan Road, Nankai District Tianjin, Tianjin 300193, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huo
authors.affiliation: Tianjin University of Traditional Chinese Medicine, 312 Yuquan Road, Nankai District Tianjin, Tianjin 300193, China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Tianjin University of Traditional Chinese Medicine, 312 Yuquan Road, Nankai District Tianjin, Tianjin 300193, China.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Hou
authors.affiliation: Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, 88 Yuquan Road, Nankai District, Tianjin 300193, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Han
authors.affiliation: Tianjin University of Traditional Chinese Medicine, 312 Yuquan Road, Nankai District Tianjin, Tianjin 300193, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, 88 Yuquan Road, Nankai District, Tianjin 300193, China. liuwei628@tjutcm.edu.cn.
authors.forename: Erwei
authors.initials: E
authors.lastname: Liu
authors.affiliation: Tianjin University of Traditional Chinese Medicine, 312 Yuquan Road, Nankai District Tianjin, Tianjin 300193, China. gaoxiumei@tjutcm.edu.cn.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007408:Intestinal Absorption / Q000187:drug effects*; D057888:Iridoid Glucosides / Q000378:metabolism*; D039823:Iridoids / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D029907:Psoralea / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D057888:Iridoid Glucosides; D039823:Iridoids; D010936:Plant Extracts; C007835:geniposide; C058966:geniposidic acid