title: Targeting SIM2-s decreases glioma cell invasion through mesenchymal--epithelial transition.
abstract: Glioma is a common primary intracranial carcinoma with high incidence, recurrence, and motility. Single minded homolog 2-short form (SIM2-s), a member of basic helix-loop-helix (bHLH) family, is reported to be expressed in glioma and might play a role in the invasion. In the present study, we investigated the importance of SIM2-s in glioma invasion and further explored the potential mechanisms. We showed that targeting SIM2-s by interference technology could decrease cell adhesion to fibronectin, induce cell aggregation and cytoskeletal changes. Furthermore, we showed that targeting SIM2-s increased the expression of epithelial markers and decreased the expression of mesenchymal markers, that is mesenchymal-epithelial transition (MET). Targeting SIM2-s decreased self-renewal of glioma stem cells by tumor sphere formation assay. Taken together, our results indicated that MET is involved in the inhibition of glioma invasion by targeting SIM2-s, and SIM2-s may be a new gene target.
authors.affiliation: Department of Emergency, Qi Lu Hospital, Shandong University, 250012, Jinan, China; Department of Neurosurgery, Qi Lu Hospital, Shandong University, 250012, Jinan, China.
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Su
authors.forename: Juntao
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Lin
authors.initials: L
authors.lastname: Deng
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Li
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005353:Fibronectins / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D034741:RNA, Small Interfering / Q000494:pharmacology*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D005353:Fibronectins; D034741:RNA, Small Interfering; C417032:SIM2 protein, human
keywords: GLIOMA; INVASION; MET; SIM2-s