title: In vitro thrombolytic activity of purified streptokinase extracted from Streptococcus equinus VIT_VB2 isolated from bovine milk.
abstract: Streptokinase (SK) is an extracellular enzyme secreted by various strains of β-hemolytic Streptococci. The main focus of the current study is to evaluate the in vitro thrombolytic activity of purified SK extracted from Streptococcus equinus VIT_VB2 (Accession no. JX406835) isolated from milk sample. The growth rate of S. equinus VIT_VB2 strain was studied with pH and biomass content which has positive significant effect on enzyme yield. A temperature of 10 °C and pH of 6 was found to be optimum for maximum SK activity. The specific activity of the purified SK produced by VIT_VB2 strain was found to be 6,585 IU mg(-1). The molecular mass of the enzyme was determined as 47 kDa by SDS-PAGE. In vitro thrombolytic activity of purified SK was determined using synthetic chromogenic substrate S-2251, the activity of the purified enzyme was found to be 6,330 ± 2.2 IU. The purity of SK was compared with standard SK by HPLC. This is the first report which reveals the SK activity of S. equinus isolated from milk sample.
authors.affiliation: Industrial Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore, 632 014, Tamil Nadu, India.
authors.forename: Vaishnavi
authors.initials: V
authors.lastname: Babu
authors.forename: C
authors.initials: C
authors.lastname: Subathra Devi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002417:Cattle; D005260:Female; D005342:Fibrinolysis / Q000187:drug effects*; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D008297:Male; D008892:Milk / Q000382:microbiology*; D018502:Streptococcus equi / Q000201:enzymology / Q000302:isolation & purification; D013300:Streptokinase / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D005343:Fibrinolytic Agents; D013300:Streptokinase