title: Enantioselective DNA condensation induced by heptameric lanthanum helical supramolecular enantiomers.
abstract: DNA condensation induced by a pair of heptameric La(III) helical enantiomers M-[La7(S-L)6(CO3)(NO3)6(OCH3)(CH3OH)7]·2CH3OH·5H2O and P-[La7(R-L)6(CO3)(NO3)6(OCH3)(CH3OH)5(H2O)2]·2CH3OH·4H2O (M-La and P-La, L=2-(2-hydroxybenzylamino)-3-carbamoylpropanoic acid) has been investigated by UV/vis spectroscopy, fluorescence spectroscopy, CD spectroscopy, EMSA, RALS, DLS, and SEM. The enantiomers M-La and P-La could induce CT-DNA condensation at a low concentration as observed in UV/vis spectroscopy. DNA condensates possessed globular nanoparticles with nearly homogeneous sizes in solid state determined by SEM (ca. 250 nm for M-La and ca. 200 nm for P-La). The enantiomers bound to DNA through electrostatic attraction and hydrogen bond interactions in a major groove, and rapidly condensed free DNA into its compact state. DNA decompaction has been acquired by using EDTA as disassembly agent, and analyzed by UV/vis spectroscopy, CD spectroscopy and EMSA. Moreover, the enantiomers M-La and P-La displayed discernible discrimination in DNA interaction and DNA condensation, as well as DNA decondensation. Our study suggested that lanthanum(III) enantiomers M-La and P-La were efficient DNA packaging agents with potential applications in gene delivery.
authors.affiliation: School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Bao
authors.affiliation: School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Xu
authors.affiliation: School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhou
authors.affiliation: School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Pang
authors.affiliation: School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Zaijun
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China; The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China. Electronic address: zhiguogu@jiangnan.edu.cn.
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Gu
mesh_terms: D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D007811:Lanthanum / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet; D013057:Spectrum Analysis; D013237:Stereoisomerism
chemical_list: D056831:Coordination Complexes; D007811:Lanthanum; D004247:DNA; C427203:calf thymus DNA
keywords: DNA condensation; Enantioselective; Lanthanum; Supramolecular cylinder enantiomers