title: An ionic liquid-modified graphene based molecular imprinting electrochemical sensor for sensitive detection of bovine hemoglobin.
abstract: A novel kind of molecular imprinted polymers based on ionic liquid-functionalized graphene (MIPs/IL/GR) was prepared by electro-polymerization, which was applied as a molecular recognition element to modify glassy carbon electrode (GCE) to construct an electrochemical sensor (MIPs/IL/GR/GCE) for sensitive detection of bovine hemoglobin (BHb). The fabrication conditions that affect the performance of the imprinted sensor, such as pyrrole concentration, scan cycles and scan rates, have been discussed. Under the optimized conditions, the prepared molecular imprinting electrochemical sensor showed a fast rebinding dynamics, which was successfully applied to BHb detection with a wide linear range from 1.0 × 10(-10) to 1.0 × 10(-3)g/L (R=0.998) and a detection limit of 3.09 × 10(-11)g/L. Moreover, the fabricated sensor possessed a good selectivity and stability, providing a promising tool for immunoassays and clinical applications.
authors.affiliation: Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China. Electronic address: wangzonghua@qdu.edu.cn.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Xia
authors.affiliation: Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xia
authors.affiliation: Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Sai
authors.initials: S
authors.lastname: Bi
authors.affiliation: Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Guoyu
authors.initials: G
authors.lastname: Shi
authors.affiliation: Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Yanzhi
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Jingquan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Li
authors.affiliation: Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Linhua
authors.initials: L
authors.lastname: Xia
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry; D002417:Cattle; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006108:Graphite / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis*; D052578:Ionic Liquids / Q000737:chemistry*; D057230:Limit of Detection; D054802:Molecular Imprinting; D058105:Polymerization
chemical_list: D006454:Hemoglobins; D052578:Ionic Liquids; D002244:Carbon; D006108:Graphite
keywords: Bovine hemoglobin; Electrochemical sensor; Graphene; Ionic liquid; Molecular imprinting technology