title: Structures and binding specificity of galactose- and mannose-binding lectins from champedak: differences from jackfruit lectins.
abstract: Galactose-binding and mannose-binding lectins from the champedak fruit, which is native to South-east Asia, exhibit useful potential clinical applications. The specificity of the two lectins for their respective ligands allows the detection of potential cancer biomarkers and monitoring of the glycosylated state of proteins in human serum and/or urine. To fully understand and expand the use of these natural proteins, their complete sequences and crystal structures are presented here, together with details of sugar binding.
authors.affiliation: Institute of Molecular, Cell and Systems Biology, University of Glasgow, 120 University Avenue, Glasgow G12 8TA, Scotland.
authors.forename: Mads
authors.initials: M
authors.lastname: Gabrielsen
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Puteri Shafinaz
authors.initials: PS
authors.lastname: Abdul-Rahman
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Shatrah
authors.initials: S
authors.lastname: Othman
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Onn H
authors.initials: OH
authors.lastname: Hashim
authors.affiliation: Institute of Molecular, Cell and Systems Biology, University of Glasgow, 120 University Avenue, Glasgow G12 8TA, Scotland.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Cogdell
mesh_terms: D000595:Amino Acid Sequence; D031622:Artocarpus / Q000737:chemistry*; D014408:Biomarkers, Tumor / Q000097:blood / Q000652:urine; D003460:Crystallization; D005690:Galactose / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D014408:Biomarkers, Tumor; D037102:Lectins; D008358:Mannose; D005690:Galactose
keywords: champedak; galactose-binding lectin; mannose-binding lectin