title: Molecular methods for microbiological quality control of meat and meat products: a review.
abstract: Achieving food safety is a global health goal and the food-borne diseases take a major check on global health. Therefore, detection of microbial pathogens in food is the solution to the prevention and recognition of problems related to health and safety. Conventional and standard bacterial detection methods such as culture and colony counting methods and immunology-based methods may take up to several hours or even a few days to yield a result. Obviously, this is inadequate, and recently many researchers are focusing towards the progress of rapid diagnostic methods. The advent of molecular techniques has led to the development of a diverse array of assay for quality control of meat and meat products. Rapid analysis using DNA hybridization and amplification techniques offer more sensitivity and specificity to get results than culture based methods as well as dramatic reduction in the time to get results. Many methods have also achieved the high level automation, facilitating their application as routine sample screening assays. This review is intended to provide an overview of the molecular methods for microbiological quality control of meat and meat products.
authors.affiliation: a Division of Livestock Products Technology , Indian Veterinary Research Institute , Izatnagar, Bareilly , UP , India.
authors.forename: P
authors.initials: P
authors.lastname: Gokulakrishnan
authors.forename: Jess
authors.initials: J
authors.lastname: Vergis
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology / Q000379:methods; D008460:Meat / Q000032:analysis / Q000382:microbiology*; D008461:Meat Products / Q000032:analysis*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011786:Quality Control
chemical_list: D004247:DNA
keywords: DNA analysis; Meat; PCR; meat products; molecular methods; quality control