title: Non-union site debridement increased the efficacy of rhBMP-2 in a rodent model.
abstract: In our study we investigated the influence of debridement on bone healing in a rodent critical size defect model with and without rhBMP-2 in fibrin matrix. A total of 58 male Sprague-Dawley rats underwent a first surgical procedure where a femoral osteotomy was performed. In the single step group the defect remained empty and the specimens were collected 4 weeks later. A silicone spacer was implanted to inhibit bone healing within the defect in all the other groups. At 4 weeks the spacer was removed in a second operation with and without debridement of the bone ends and fibrin matrix alone or combined with 10 μg rhBMP-2 were applied. 4 weeks after the primary operation those specimens were collected. All the specimens were evaluated by μCT scans and histological analysis. Debridement of the defect significantly increased bone volume in the animals treated with rhBMP-2. In the control groups without growth factor application the effect of debridement was not significant concerning the union rate and the bone volume. In our experimental setting surgical debridement of the non-union site particularly promoted bone healing in combination with BMP-2 administration in fibrin matrix.
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre/Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, 1200 Vienna, Austria; AUVA Trauma Center Meidling, Kundratstraße 37, 1120 Vienna, Austria. Electronic address: sebastian.schuetzenberger@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Schützenberger
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre/Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, 1200 Vienna, Austria; Orthopaedic Department, Barmherzige Brüder Hospital, Esterhazystrasse 26, 7000 Eisenstadt, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Kaipel
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre/Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, 1200 Vienna, Austria; AUVA Trauma Center Lorenz Böhler, Donaueschingenstrasse 13, 1200 Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Schultz
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre/Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, 1200 Vienna, Austria.
authors.forename: T
authors.initials: T
authors.lastname: Nau
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre/Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, 1200 Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Redl
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre/Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, 1200 Vienna, Austria; Paracelsus Private University/Department for Trauma Surgery, Strubergasse 21, 5020 Salzburg, Austria.
authors.forename: T
authors.initials: T
authors.lastname: Hausner
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D003646:Debridement / Q000379:methods; D004195:Disease Models, Animal; D005264:Femoral Fractures / Q000473:pathology / Q000503:physiopathology*; D005269:Femur / Q000473:pathology*; D005337:Fibrin / Q000494:pharmacology; D017102:Fracture Healing; D005599:Fractures, Ununited / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D010027:Osteotomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D016896:Treatment Outcome
chemical_list: C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; D005337:Fibrin
keywords: BMP-2; Debridement; Fibrin matrix; Non-union; Rat; Rodent model