title: Urinary ACE2 in healthy adults and patients with uncomplicated type 1 diabetes.
abstract: Angiotensin-converting enzyme 2 (ACE2) is expressed in the kidney and may be renoprotective. We determined whether urinary ACE2 enzyme activity and protein levels (ELISA), as well as angiotensinogen and ACE, are elevated during clamped euglycemia (4-6 mmol·L(-1)) in patients with uncomplicated type 1 diabetes (T1D, n = 58) compared with normoglycemic controls (n = 21). We also measured the effect of clamped hyperglycemia (9-11 mmol·L(-1)) on each urinary factor in T1D patients. Urinary ACE2 activity and protein levels were higher during clamped euglycemia in T1D compared with the controls (p < 0.0001). In contrast, urinary angiotensinogen levels (p = 0.27) and ACE excretion (p = 0.68) did not differ. In response to clamped hyperglycemia in T1D, urinary ACE2 protein decreased (p < 0.0001), whereas urinary ACE2 activity as well as angiotensinogen and ACE levels remained unchanged. Urinary ACE2 activity and protein expression are increased in T1D patients prior to the onset of clinical complications. Further work is required to determine the functional role of urinary ACE2 in early T1D.
authors.affiliation: a Division of Nephrology, Department of Medicine, University Health Network, University of Toronto, Toronto, Ontario, Canada.
authors.forename: David Z I
authors.initials: DZ
authors.lastname: Cherney
authors.forename: Fengxia
authors.initials: F
authors.lastname: Xiao
authors.forename: Joseph
authors.initials: J
authors.lastname: Zimpelmann
authors.forename: Ronnie L H
authors.initials: RL
authors.lastname: Har
authors.forename: Vesta
authors.initials: V
authors.lastname: Lai
authors.forename: James W
authors.initials: JW
authors.lastname: Scholey
authors.forename: Heather N
authors.initials: HN
authors.lastname: Reich
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Burns
mesh_terms: D000328:Adult; D000085962:Angiotensin-Converting Enzyme 2; D000808:Angiotensinogen / Q000378:metabolism; D016022:Case-Control Studies; D015331:Cohort Studies; D003922:Diabetes Mellitus, Type 1 / Q000652:urine*; D005260:Female; D015309:Glucose Clamp Technique; D006801:Humans; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism / Q000652:urine*
chemical_list: D000808:Angiotensinogen; C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A; C000705307:ACE2 protein, human; D000085962:Angiotensin-Converting Enzyme 2
keywords: ECA2 urinaire; diabetes mellitus; diabète sucré; urinary ACE2