title: Gene expression profiling analysis of bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells.
abstract: Bisphenol A (BPA) is an environmental endocrine disruptor which has been detected in human bodies. Many studies have implied that BPA exposure is harmful to human health. Previous studies mainly focused on BPA effects on estrogen receptor (ER)-positive cells. Genome-wide impacts of BPA on gene expression in ER-negative cells is unclear. In this study, we performed RNA-seq to characterize BPA-induced cellular and molecular impacts on ER-negative HEK293 cells. The microscopic observation showed that low-dose BPA exposure did not affect cell viability and morphology. Gene expression profiling analysis identified a list of differentially expressed genes in response to BPA exposure in HEK293 cells. These genes were involved in variable important biological processes including ion transport, cysteine metabolic process, apoptosis, DNA damage repair, etc. Notably, BPA up-regulated the expression of ERCC5 encoding a DNA endonuclease for nucleotide-excision repair. Further electrochemical experiment showed that BPA induced significant DNA damage in ER-positive MCF-7 cells but not in ER-negative HEK293 cells. Collectively, our study revealed that ER-negative HEK293 cells employed mechanisms in response to BPA exposure different from ER-positive cells.
authors.affiliation: Department of Chemistry, Tongji University, Shanghai, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Yin
authors.affiliation: Shanghai Key Laboratory of Signaling and Disease Research, the School of Life Sciences and Technology, Tongji University, Shanghai, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Gu
authors.affiliation: Shanghai Key Laboratory of Signaling and Disease Research, the School of Life Sciences and Technology, Tongji University, Shanghai, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Shanghai Key Laboratory of Signaling and Disease Research, the School of Life Sciences and Technology, Tongji University, Shanghai, China.
authors.forename: Cizhong
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, Tongji University, Shanghai, China.
authors.forename: Tongcheng
authors.initials: T
authors.lastname: Cao
authors.affiliation: Shanghai Key Laboratory of Signaling and Disease Research, the School of Life Sciences and Technology, Tongji University, Shanghai, China.
authors.forename: Xiaobai
authors.initials: X
authors.lastname: Zhang
mesh_terms: D001559:Benzhydryl Compounds / Q000009:adverse effects / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D052244:Endocrine Disruptors / Q000009:adverse effects / Q000494:pharmacology; D004968:Estrogens, Non-Steroidal / Q000009:adverse effects / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D053263:Gene Regulatory Networks; D057809:HEK293 Cells; D006801:Humans; D061986:MCF-7 Cells; D010636:Phenols / Q000009:adverse effects / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000172:deficiency*; D059467:Transcriptome; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D011960:Receptors, Estrogen; C006780:bisphenol A; D000098826:Bisphenol A Compounds