title: Age-dependent transcriptome and proteome following transection of neonatal spinal cord of Monodelphis domestica (South American grey short-tailed opossum).
abstract: This study describes a combined transcriptome and proteome analysis of Monodelphis domestica response to spinal cord injury at two different postnatal ages. Previously we showed that complete transection at postnatal day 7 (P7) is followed by profuse axon growth across the lesion with near-normal locomotion and swimming when adult. In contrast, at P28 there is no axon growth across the lesion, the animals exhibit weight-bearing locomotion, but cannot use hind limbs when swimming. Here we examined changes in gene and protein expression in the segment of spinal cord rostral to the lesion at 24 h after transection at P7 and at P28. Following injury at P7 only forty genes changed (all increased expression); most were immune/inflammatory genes. Following injury at P28 many more genes changed their expression and the magnitude of change for some genes was strikingly greater. Again many were associated with the immune/inflammation response. In functional groups known to be inhibitory to regeneration in adult cords the expression changes were generally muted, in some cases opposite to that required to account for neurite inhibition. For example myelin basic protein expression was reduced following injury at P28 both at the gene and protein levels. Only four genes from families with extracellular matrix functions thought to influence neurite outgrowth in adult injured cords showed substantial changes in expression following injury at P28: Olfactomedin 4 (Olfm4, 480 fold compared to controls), matrix metallopeptidase (Mmp1, 104 fold), papilin (Papln, 152 fold) and integrin α4 (Itga4, 57 fold). These data provide a resource for investigation of a priori hypotheses in future studies of mechanisms of spinal cord regeneration in immature animals compared to lack of regeneration at more mature stages.
authors.affiliation: Department of Pharmacology & Therapeutics, The University of Melbourne, Victoria, Australia.
authors.forename: Norman R
authors.initials: NR
authors.lastname: Saunders
authors.affiliation: Department of Pharmacology & Therapeutics, The University of Melbourne, Victoria, Australia.
authors.forename: Natassya M
authors.initials: NM
authors.lastname: Noor
authors.affiliation: Department of Pharmacology & Therapeutics, The University of Melbourne, Victoria, Australia.
authors.forename: Katarzyna M
authors.initials: KM
authors.lastname: Dziegielewska
authors.affiliation: Department of Pharmacology & Therapeutics, The University of Melbourne, Victoria, Australia.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Wheaton
authors.affiliation: Department of Pharmacology & Therapeutics, The University of Melbourne, Victoria, Australia; Department of Neurobiology, Stanford University, Stanford, California, United States of America.
authors.forename: Shane A
authors.initials: SA
authors.lastname: Liddelow
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: David L
authors.initials: DL
authors.lastname: Steer
authors.affiliation: Department of Neuroscience and Physiology, University of Gothenburg, Gothenburg, Sweden.
authors.forename: C Joakim
authors.initials: CJ
authors.lastname: Ek
authors.affiliation: Department of Pharmacology & Therapeutics, The University of Melbourne, Victoria, Australia.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Habgood
authors.affiliation: Walter & Eliza Hall Institute of Medical Research, Victoria, Australia; Department of Genetics, The University of Melbourne, Victoria, Australia.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Wakefield
authors.affiliation: Walter & Eliza Hall Institute of Medical Research, Victoria, Australia; Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
authors.forename: Helen
authors.initials: H
authors.lastname: Lindsay
authors.affiliation: The Miami Project to Cure Paralysis, University of Miami, Miller School of Medicine, Miami, Florida, United States of America.
authors.forename: Jessie
authors.initials: J
authors.lastname: Truettner
authors.affiliation: Center for Evolutionary & Theoretical Immunology, Department of Biology, University of New Mexico, Albuquerque, New Mexico, United States of America.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Miller
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: A Ian
authors.initials: AI
authors.lastname: Smith
authors.affiliation: The Miami Project to Cure Paralysis, University of Miami, Miller School of Medicine, Miami, Florida, United States of America.
authors.forename: W Dalton
authors.initials: WD
authors.lastname: Dietrich
mesh_terms: D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D063990:Gene Ontology; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D048309:Monodelphis / Q000235:genetics* / Q000254:growth & development*; D009186:Myelin Sheath / Q000378:metabolism; D009929:Organ Size / Q000235:genetics; D020543:Proteome / Q000235:genetics*; D040901:Proteomics; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology; D013119:Spinal Cord Injuries / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D016326:Extracellular Matrix Proteins; D053583:Interleukin-1beta; D020543:Proteome