title: Assessment of genotoxic effects of lead in occupationally exposed workers.
abstract: The genotoxicological effects in 200 lead acid storage battery recycling and manufacturing industry workers in Hyderabad along with matched 200 controls were studied. The genetic damage was determined by comet, micronucleus (MN), and chromosomal aberration (CA) test in peripheral blood lymphocytes (PBL). The MN test was also carried out in buccal epithelial cells (BECs). Pb in ambient air, blood Pb (B-Pb) concentrations, and hematological parameters were measured. The superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GPx), and malondialdehyde (MDA) formed were also studied. The results of the present study showed that there was a statistically significant (P < 0.01) increase in mean percent tail DNA, frequency of CA, and MN in PBL as well as in BEC as compared to controls. Pb in ambient air and B-Pb concentrations were found to be significantly higher (P < 0.01). The hematocrit, hemoglobin, and red blood cell values were significantly lowered in Pb-exposed workers in comparison to controls. SOD, GPx, and CAT levels were significantly decreased while GSH and MDA levels increased in exposed group when compared to control group. The present study suggests that environmental health standards should be enforced to control Pb contamination from battery industries to reduce human health risk.
authors.affiliation: Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Hyderabad, Andhra Pradesh, 500007, India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Chinde
authors.forename: Monika
authors.initials: M
authors.lastname: Kumari
authors.forename: Kanapuram Rudrama
authors.initials: KR
authors.lastname: Devi
authors.forename: Upadhyayula Suryanarayana
authors.initials: US
authors.lastname: Murty
authors.forename: Mohammed Fazlur
authors.initials: MF
authors.lastname: Rahman
authors.forename: Srinivas Indu
authors.initials: SI
authors.lastname: Kumari
authors.forename: Mohammed
authors.initials: M
authors.lastname: Mahboob
authors.forename: Paramjit
authors.initials: P
authors.lastname: Grover
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000032:analysis / Q000097:blood / Q000633:toxicity*; D002374:Catalase / Q000097:blood; D002869:Chromosome Aberrations; D020552:Comet Assay; D004249:DNA Damage; D011211:Electric Power Supplies; D004784:Environmental Monitoring; D004847:Epithelial Cells / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007194:India; D007854:Lead / Q000032:analysis / Q000097:blood / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D015162:Micronucleus Tests; D008875:Middle Aged; D009153:Mutagens / Q000032:analysis / Q000633:toxicity*; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis; D059027:Recycling; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000395:Air Pollutants, Occupational; D009153:Mutagens; D007854:Lead; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase