title: Neuroprotective effects of 4,5-dimethoxypyrocatechol isolated from Cynanchum paniculatum on HT22 cells.
abstract: BACKGROUND
  Cynanchum paniculatum, belongs to the family Asclepiadaceae and is used to treat various diseases, such as invigorate blood, alleviate edema and to relieve pain and toxicity for a long time.
  
  
  METHODS
  4,5-Dimethoxypyrocatechol was isolated from the 80% methanol extract of C. paniculatum and its neuroprotective effect was evaluated by MTT assay.
  
  
  RESULTS
  4,5-Dimethoxypyrocatechol had neuroprotective effect on the glutamate-induced cellular oxidative death in HT22 cells.
  
  
  CONCLUSIONS
  Furthermore, we found that reactive oxygen species (ROS) accumulation and calcium concentration by oxidative stress were reduced by 4,5-dimethoxypyrocatechol in HT22 cells.
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Korea.
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Korea.
authors.forename: Bohyoung
authors.initials: B
authors.lastname: Lee
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Korea.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Yun
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Korea.
authors.forename: Jiwoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Korea ; Research Institute of Biotechnology, Kangwon National University, Chuncheon, Korea.
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
keywords: 4,5-dimethoxypyrocatechol; Cynanchum paniculatum; HT22 cells; neuroprotective effect