title: Growth promoting effect of hyaluronan synthesis promoting substances on Japanese eel leptocephali.
abstract: Hyaluronans (HAs) are glycosaminoglycans produced in the bodies of Anguilliform and Elopiform leptocephali, and play a role in metabolic energy. In mammals, HA synthesis-promoting substances (HASPS) up-regulate the expression of HA synthase (HAS) and increase the amount of HA in the body. In this study, Japanese eel leptocephali were fed a HASPS containing diet. We analyzed HAS1s and HAS2 expression, HA content, and their influence on growth. HASPS extracted from Grifola frondosa promoted HAS1s and HAS2 mRNA and HA content. Other than mammals, these results are first reported in vertebrate. Moreover, HASPS extracted from G. frondosa promoted leptocephalus growth. The relationship between growth and HA in the leptocephali is not yet clear. However, based on our results we hypothesize that HA is involved in the storage of energy, which is metabolized to sugars when needed for metabolic energy.
authors.affiliation: Nansei Station, National Research Institute of Aquaculture, Fisheries Research Agency, Minamiise, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawakami
authors.affiliation: Nansei Station, National Research Institute of Aquaculture, Fisheries Research Agency, Minamiise, Japan.
authors.forename: Kazuharu
authors.initials: K
authors.lastname: Nomura
authors.affiliation: Nansei Station, National Research Institute of Aquaculture, Fisheries Research Agency, Minamiise, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D053898:Biosynthetic Pathways; D003001:Cloning, Molecular; D004524:Eels / Q000145:classification / Q000502:physiology*; D015870:Gene Expression; D014453:Glucuronosyltransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D008297:Male; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D012333:RNA, Messenger; D006820:Hyaluronic Acid; D014453:Glucuronosyltransferase