title: Growth dynamics of geographically different arbuscular mycorrhizal fungal isolates belonging to the 'Rhizophagus clade' under monoxenic conditions.
abstract: The growth dynamics of extraradical mycelium and spore formation of 14 "Rhizophagus" isolates from different sites in Argentina were evaluated under monoxenic conditions. A modified Gompertz model was used to characterize the development of mycelium and spores for each isolate under the same conditions. The lag time, maximal growth rate and total quantity of both extraradical hyphae and spores were determined. Wide variability among isolates was detected, and all growth parameters were significantly altered by fungal isolate. Discriminant analysis differentiated isolates primarily based on the extent of extraradical hyphae produced, yet such differences did not conclusively correspond to phylogenetic relationships among closely related isolates based on partial SSU sequences. Given that the "Rhizophagus" isolates were grown under controlled conditions for many generations, the expression of phenotypic variability could be attributed to genetic differences that are not completely resolved by phylogenetic analysis employing the small ribosomal gene.
authors.affiliation: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Ciudad Universitaria, 4to piso, Pabellón 2, 1428, Buenos Aires, Argentina vanesasilvani@gmail.com.
authors.forename: Vanesa Analía
authors.initials: VA
authors.lastname: Silvani
authors.affiliation: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Ciudad Universitaria, 4to piso, Pabellón 2, 1428, Buenos Aires, Argentina.
authors.forename: Laura Fernández
authors.initials: LF
authors.lastname: Bidondo
authors.affiliation: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Ciudad Universitaria, 4to piso, Pabellón 2, 1428, Buenos Aires, Argentina.
authors.forename: María Josefina
authors.initials: MJ
authors.lastname: Bompadre
authors.affiliation: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Ciudad Universitaria, 4to piso, Pabellón 2, 1428, Buenos Aires, Argentina.
authors.forename: Roxana Paula
authors.initials: RP
authors.lastname: Colombo
authors.affiliation: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Ciudad Universitaria, 4to piso, Pabellón 2, 1428, Buenos Aires, Argentina.
authors.forename: Mariana
authors.initials: M
authors.lastname: Pérgola
authors.affiliation: SAP Labs, 1251 Waterfront Place, Pittsburgh, Pennsylvania 15222.
authors.forename: Agustín
authors.initials: A
authors.lastname: Bompadre
authors.affiliation: Centro Regional de Investigaciones Científicas y Transferencia Tecnológica, CRILAR-CONICET, Entre Ríos y Mendoza, 530 Anillaco, La Rioja, Argentina.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Fracchia
authors.affiliation: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Ciudad Universitaria, 4to piso, Pabellón 2, 1428, Buenos Aires, Argentina.
authors.forename: Alicia
authors.initials: A
authors.lastname: Godeas
mesh_terms: D001118:Argentina; D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D055137:Glomeromycota / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D008969:Molecular Sequence Data; D025282:Mycelium; D038821:Mycorrhizae / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal
keywords: Glomeromycota; R. irregularis; Rhizophagus intraradices; growth dynamics; in vitro; variability