title: Cell cycle synchronization and flow cytometry analysis of mammalian cells.
abstract: Analysis of cellular DNA content and measurement of pulse-labeled newly replicated DNA by flow cytometry are useful techniques for cell cycle studies. In this chapter, we describe the protocols for cell cycle synchronization of mammalian cells, including time course designs and consideration of cell types to achieve successful experiments, along with the methods for detection of DNA. Some selected applications dealing with siRNA-mediated knockdown are also presented.
authors.affiliation: Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Yoshizawa-Sugata
authors.forename: Hisao
authors.initials: H
authors.lastname: Masai
mesh_terms: D000818:Animals; D002453:Cell Cycle; D004247:DNA / Q000032:analysis / Q000235:genetics; D004261:DNA Replication; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D004247:DNA