title: Organ accumulation and subcellular location of Cicer arietinum ST1 protein.
abstract: The ST (ShooT Specific) proteins are a new family of proteins characterized by a signal peptide, tandem repeats of 25/26 amino acids, and a domain of unknown function (DUF2775), whose presence is limited to a few families of dicotyledonous plants, mainly Fabaceae and Asteraceae. Their function remains unknown, although involvement in plant growth, fruit morphogenesis or in biotic and abiotic interactions have been suggested. This work is focused on ST1, a Cicer arietinum ST protein. We established the protein accumulation in different tissues and organs of chickpea seedlings and plants and its subcellular localization, which could indicate the possible function of ST1. The raising of specific antibodies against ST1 protein revealed that its accumulation in epicotyls and radicles was related to their elongation rate. Its pattern of tissue location in cotyledons during seed formation and early seed germination, as well as its localization in the perivascular fibres of epicotyls and radicles, indicated a possible involvement in seed germination and seedling growth. ST1 protein appears both inside the cell and in the cell wall. This double subcellular localization was found in every organ in which the ST1 protein was detected: seeds, cotyledons and seedling epicotyls and radicles.
authors.affiliation: Dpto de Fisiología Vegetal. Facultad de Biología, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Pza Doctores de la Reina s/n., Salamanca 37007, Spain. Electronic address: lucialbornos@usal.es.
authors.forename: Lucía
authors.initials: L
authors.lastname: Albornos
authors.affiliation: Dpto de Fisiología Vegetal. Facultad de Biología, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Pza Doctores de la Reina s/n., Salamanca 37007, Spain. Electronic address: Javier.CabreraChaves@uclm.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Cabrera
authors.affiliation: Dpto de Fisiología Vegetal, Universidad de Santiago de Compostela, Campus de Lugo, Lugo 27002, Spain. Electronic address: josefina.hernandez@usc.es.
authors.forename: Josefina
authors.initials: J
authors.lastname: Hernández-Nistal
authors.affiliation: Dpto de Fisiología Vegetal. Facultad de Biología, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Pza Doctores de la Reina s/n., Salamanca 37007, Spain. Electronic address: a56562@usal.es.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Martín
authors.affiliation: Dpto de Fisiología Vegetal. Facultad de Biología, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Pza Doctores de la Reina s/n., Salamanca 37007, Spain. Electronic address: labrador@usal.es.
authors.forename: Emilia
authors.initials: E
authors.lastname: Labrador
authors.affiliation: Dpto de Fisiología Vegetal. Facultad de Biología, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Pza Doctores de la Reina s/n., Salamanca 37007, Spain. Electronic address: bdr@usal.es.
authors.forename: Berta
authors.initials: B
authors.lastname: Dopico
mesh_terms: D002473:Cell Wall / Q000378:metabolism*; D029863:Cicer / Q000254:growth & development / Q000378:metabolism*; D018548:Cotyledon / Q000254:growth & development / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D018525:Germination; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism*; D012639:Seeds / Q000254:growth & development / Q000378:metabolism*
chemical_list: D010940:Plant Proteins
keywords: Cell wall; Chickpea; DUF2775; Germination; Growth; ST proteins