title: Enzymatic process for the fractionation of baker's yeast cell wall (Saccharomyces cerevisiae).
abstract: β-Glucans, homopolymers of glucose, are widespread in many microorganisms, mushrooms and plants. They have attracted attention because of their bioactive and medicinal functions. One important source of β-glucans is the cell wall of yeasts, especially that of baker's yeast Saccharomyces cerevisiae. Several processes for the isolation of β-glucans, using alkali, acid or a combination of both, result in degradation of the polymeric chains. In this paper, we have an enzymatic process for the isolation of glucans from yeast cell walls. As a result, β-glucans were obtained in a yield of 18.0% of the original ratio in the yeast cell walls. Therefore, this isolation process gave a better yield and higher β-glucan content than did traditional isolation methods. Furthermore, results showed that each extraction step of β-glucan had a significant effects on its chemical properties.
authors.affiliation: Université de Liège, Gembloux Agro-Bio Tech, Unité de Science des Aliments et Formulation, Passage des Déportés 2, B-5030 Gembloux, Belgium. Electronic address: borchani_chema@yahoo.fr.
authors.forename: Chema
authors.initials: C
authors.lastname: Borchani
authors.affiliation: Université de Liège, Gembloux Agro-Bio Tech, Unité de Bioindustries, Passage des Déportés 2, B-5030 Gembloux, Belgium.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Fonteyn
authors.affiliation: Université de Liège, Gembloux Agro-Bio Tech, Unité de Bioindustries, Passage des Déportés 2, B-5030 Gembloux, Belgium.
authors.forename: Guilhem
authors.initials: G
authors.lastname: Jamin
authors.affiliation: Université de Liège, Gembloux Agro-Bio Tech, Unité de Chimie Analytique, Passage des Déportés 2, 5030 Gembloux, Belgium.
authors.forename: Michel
authors.initials: M
authors.lastname: Paquot
authors.affiliation: Université de Liège, Gembloux Agro-Bio Tech, Unité de Science des Aliments et Formulation, Passage des Déportés 2, B-5030 Gembloux, Belgium.
authors.forename: Christophe
authors.initials: C
authors.lastname: Blecker
authors.affiliation: Université de Liège, Gembloux Agro-Bio Tech, Unité de Bioindustries, Passage des Déportés 2, B-5030 Gembloux, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Thonart
mesh_terms: D002473:Cell Wall / Q000737:chemistry; D005591:Chemical Fractionation; D008903:Minerals / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D012697:Serine Endopeptidases / Q000494:pharmacology; D047071:beta-Glucans / Q000032:analysis
chemical_list: D008903:Minerals; D047071:beta-Glucans; D012697:Serine Endopeptidases; C032923:microbial serine proteinases
keywords: Baker’s yeast; Chemical properties; Enzymatic process; Saccharomyces cerevisiae; Yield; β-Glucans