title: Analysis of IL-17 production by flow cytometry and ELISPOT assays.
abstract: Interleukin (IL)-17 represents a family of cytokines with six members, namely IL-17A, B, C, D, E, and F. IL-17A and IL-17F are best studied proinflammatory cytokines. CD4(+) T helper cells producing IL-17A have been identified as a distinct T helper subset, Th17 cells. IL-17 and Th17 cells are important mediators in tissue inflammation in immune-mediated inflammatory diseases. IL-17 is also produced by other immune cells and plays an important role in host defense against microbial infection. Cell-based assays are sensitive and quantitative, and enable identification of cellular sources of IL-17 production. This chapter describes usage of flow cytometry and ELISPOT assays to quantify IL-17A-producing cells in disease and in vitro experiments to study T cell function.
authors.affiliation: Department of Rheumatology, The First Hospital, Jinlin University Normal Bethune Medical College, Changchun, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chou
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Jiang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Jiang
authors.forename: Cong-Qiu
authors.initials: CQ
authors.lastname: Chu
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D020126:Brefeldin A / Q000494:pharmacology; D058501:Enzyme-Linked Immunospot Assay / Q000379:methods*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D020381:Interleukin-17 / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D015850:Interleukin-6 / Q000494:pharmacology; D015759:Ionomycin / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D010799:Phycoerythrin; D011806:Quinolinium Compounds; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D058504:Th17 Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: C481052:Alexa fluor 546; D000906:Antibodies; D005456:Fluorescent Dyes; C508717:IL17A protein, human; D020381:Interleukin-17; D015850:Interleukin-6; D011806:Quinolinium Compounds; D016212:Transforming Growth Factor beta; D010799:Phycoerythrin; D020126:Brefeldin A; D015759:Ionomycin; D016650:Fluorescein-5-isothiocyanate; D013755:Tetradecanoylphorbol Acetate