title: Restricted access chiral stationary phase synthesized via reversible addition-fragmentation chain-transfer polymerization for direct analysis of biological samples by high performance liquid chromatography.
abstract: Novel hydrophilic microparticles containing β-cyclodextrin (β-CD) were prepared via one-pot synthesis using reversible addition-fragmentation chain-transfer (RAFT) precipitation polymerization, a "controlled/living" radical polymerization technique. The polymerization was initiated by hydrophilic macromolecular chain-transfer agent [poly(2-hydroxyethyl methacrylate), PHEMA]. The hydrophilic PHEMA on the surface of microparticles can well improve their surface hydrophilicity and lead to their biological compatibility. As chiral restricted access material (RAM), the hydrophilic microparticles can be used for determination of enantiomers in biological samples with direct injection via HPLC analysis.
authors.affiliation: Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China. Electronic address: songwenjun@tjcu.edu.cn.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Song
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address: jiping1023@163.com.
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Wei
authors.affiliation: Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China. Electronic address: wsying@tjcu.edu.cn.
authors.forename: Su-Ying
authors.initials: SY
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. Electronic address: wanghuai1234@gmail.com.
authors.forename: Huai-Song
authors.initials: HS
authors.lastname: Wang
mesh_terms: D000818:Animals; D011232:Chemical Precipitation; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008892:Milk / Q000737:chemistry; D015394:Molecular Structure; D010575:Pesticides / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000097:blood / Q000737:chemistry; D058105:Polymerization
chemical_list: D010575:Pesticides; D004364:Pharmaceutical Preparations
keywords: Chiral separation; High performance liquid chromatography; One-pot synthesis; Restricted access material