title: Smart phone: a popular device supports amylase activity assay in fisheries research.
abstract: Colourimetric determinations of amylase activity were developed based on a standard dinitrosalicylic acid (DNS) staining method, using maltose as the analyte. Intensities and absorbances of red, green and blue (RGB) were obtained with iPhone imaging and Adobe Photoshop image analysis. Correlation of green and analyte concentrations was highly significant, and the accuracy of the developed method was excellent in analytical performance. The common iPhone has sufficient imaging ability for accurate quantification of maltose concentrations. Detection limits, sensitivity and linearity were comparable to a spectrophotometric method, but provided better inter-day precision. In quantifying amylase specific activity from a commercial source (P>0.02) and fish samples (P>0.05), differences compared with spectrophotometric measurements were not significant. We have demonstrated that iPhone imaging with image analysis in Adobe Photoshop has potential for field and laboratory studies of amylase.
authors.affiliation: Department of Applied Science, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand; Biochemical Research Unit for Feed Utilisation Assessment, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. Electronic address: karun.t@psu.ac.th.
authors.forename: Karun
authors.initials: K
authors.lastname: Thongprajukaew
authors.affiliation: Department of Applied Science, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand.
authors.forename: Aree
authors.initials: A
authors.lastname: Choodum
authors.affiliation: Department of Applied Science, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand.
authors.forename: Barunee
authors.initials: B
authors.lastname: Sa-E
authors.affiliation: Department of Applied Science, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand.
authors.forename: Ummah
authors.initials: U
authors.lastname: Hayee
mesh_terms: D000681:Amylases / Q000378:metabolism*; D000818:Animals; D040421:Cell Phone; D003124:Colorimetry / Q000379:methods; D005398:Fisheries; D008320:Maltose / Q000032:analysis
chemical_list: D008320:Maltose; D000681:Amylases
keywords: Amylase activity; Digital image analysis; Fisheries research; Smart phone