title: Protective effect of alpha glucosyl hesperidin (G-hesperidin) on chronic vanadium induced testicular toxicity and sperm nuclear DNA damage in male Sprague Dawley rats.
abstract: The study was conducted to evaluate the vanadium-induced testicular toxicity and its effect on sperm parameters, sperm nuclear DNA damage and histological alterations in Sprague Dawley rats and to assess the protective effect of G-hesperidin against this damage. Treatment of rats with vanadium at a dose of 1 mg kg bw(-1) for 90 days resulted in significant reduction in serum testosterone levels, sperm count and motility. Further, a parallel increase in abnormal sperm morphology and adverse histopathological changes in testis was also associated with vanadium administration when compared to normal control. Moreover, sperm chromatin dispersion assay revealed that vanadium induces sperm nuclear DNA fragmentation. A marked increase in testicular malondialdehyde levels and decreased activity of antioxidant enzymes such as superoxide dismutase and catalase indicates vanadium-induced oxidative stress. Co-administration of G-hesperidin at a dose of 25 and 50 mg kg bw(-1) significantly attenuated the sperm parameters and histological changes by restoring the antioxidant levels in rat testis. These results suggested that vanadium exposure caused reduced bioavailability of androgens to the tissue and increased free radical formation, thereby causing structural and functional changes in spermatozoa. G-hesperidin exhibited antioxidant effect by protecting the rat testis against vanadium-induced oxidative damage, further ensures antioxidant potential of bioflavonoids.
authors.affiliation: Pharmacology Division, University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, India.
authors.forename: B
authors.initials: B
authors.lastname: Vijaya Bharathi
authors.forename: G
authors.initials: G
authors.lastname: Jaya Prakash
authors.forename: K M
authors.initials: KM
authors.lastname: Krishna
authors.forename: C H
authors.initials: CH
authors.lastname: Ravi Krishna
authors.forename: T
authors.initials: T
authors.lastname: Sivanarayana
authors.forename: K
authors.initials: K
authors.lastname: Madan
authors.forename: G A
authors.initials: GA
authors.lastname: Rama Raju
authors.forename: A
authors.initials: A
authors.lastname: Annapurna
mesh_terms: D000818:Animals; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D005960:Glucosides / Q000494:pharmacology*; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000494:pharmacology; D007248:Infertility, Male / Q000139:chemically induced; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D013733:Testicular Diseases / Q000139:chemically induced*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D014131:Trace Elements / Q000633:toxicity*; D014639:Vanadium / Q000633:toxicity*
chemical_list: D005960:Glucosides; D014131:Trace Elements; D014639:Vanadium; C487382:glucosyl hesperidin; D008315:Malondialdehyde; D006569:Hesperidin; D002374:Catalase; D013482:Superoxide Dismutase
keywords: DNA fragmentation; G-hesperidin; male infertility; oxidative stress; vanadium toxicity