title: Dual-stimuli responsive i-motif/nanoflares for sensing ATP in lysosomes.
abstract: A dual-stimuli responsive i-motif/nanoflare for molecule detection in lysosomes was designed. By combining the structure-switchable i-motif sequence and high recognition ability of an adenosine triphosphate (ATP) aptamer, subcellular sensing and visualization sensing of ATP in lysosomes at the subcellular level can be achieved. This general sensing technique can be applied for a broad range of cellular communication studies to improve our understanding of subcellular signaling and function.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
authors.forename: Fen
authors.initials: F
authors.lastname: Jin
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.forename: Changhui
authors.initials: C
authors.lastname: Liu
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Li
authors.forename: Jishan
authors.initials: J
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Lian
authors.forename: Ronghua
authors.initials: R
authors.lastname: Yang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000737:chemistry* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D052157:Aptamers, Nucleotide; D000255:Adenosine Triphosphate