title: A simple, rapid and sensitive liquid chromatography-tandem mass spectrometry method for the determination of dienogest in human plasma and its pharmacokinetic applications under fasting.
abstract: A simple, rapid and sensitive analytical method using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) detection with positive ion electrospray ionization was developed for the determination of dienogest in human K2 EDTA plasma using levonorgestrel d6 as an internal standard (IS). Dienogest and IS were extracted from human plasma using simple liquid-liquid extraction. Chromatographic separation was achieved on a Zorbax XDB-Phenyl column (4.6 × 75 mm, 3.5 µm) under isocratic conditions using acetonitrile-5 mm ammonium acetate (70:30, v/v) at a flow rate of 0.60 mL/min. The protonated precursor to product ion transitions monitored for dienogest and IS were at m/z 312.30 → 135.30 and 319.00 → 251.30, respectively. The method was validated with a linearity range of 1.003-200.896 ng/mL having a total analysis time for each chromatograph of 3.0 min. The method has shown tremendous reproducibility with intra- and inter-day precision (coefficient of variation) <3.97 and 6.10%, respectively, and accuracy within ±4.0% of nominal values. The validated method was applied to a pharmacokinetic study in human plasma samples generated after administration of a single oral dose of 2.0 mg dienogest tablets to healthy female volunteers and was proved to be highly reliable for the analysis of clinical samples.
authors.affiliation: Bioanalytical Department, Aizant Drug Research Solutions Pvt Ltd, Sy Nos 172 and 173, Apparel Park Road, Dulapally, Hyderabad, Andhra Pradesh, 500 014, India; Department of Biotechnology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, 522510, India.
authors.forename: Leela Mohan Kumar
authors.initials: LM
authors.lastname: Pallapothu
authors.forename: Neelima
authors.initials: N
authors.lastname: Batta
authors.forename: Ravi Kumar
authors.initials: RK
authors.lastname: Pigili
authors.forename: Rajendra Prasad
authors.initials: RP
authors.lastname: Yejella
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C023635:dienogest; D009277:Nandrolone
keywords: LC-ESI-MS/MS; dienogest; method validation; pharmacokinetic study