title: Metabolomics of the rat lens: a combined LC-MS and NMR study.
abstract: This work is the first comprehensive report on the quantitative metabolomic composition of the rat lens. Quantitative metabolomic profiles of lenses were acquired with the combined use of high-frequency nuclear magnetic resonance (NMR) and high-performance liquid chromatography with high-resolution mass-spectrometric detection (LC-MS) methods. More than forty low molecular weight compounds found in the lens have been reliably identified and quantified. The most abundant metabolites in the 3-month-old Wistar rat lens are taurine, hypotaurine, lactate, phosphocholine and reduced glutathione. The analysis of age-related changes in the lens metabolomic composition shows a gradual decrease of the content of most metabolites. This decrease is the most pronounced between 1 and 3 months, which probably corresponds to the completion of the lens maturation in one-month-old rats and to the high rate of the young lens growth. The enhanced levels of tryptophan, tyrosine, carnitine, glycerophosphate, GSH and GSSG were found in lenses of senescence-accelerated OXYS rats; for some metabolites, this effect may probably be attributed to the compensatory response to oxidative stress.
authors.affiliation: International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk 630090, Russia; Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia.
authors.forename: Vadim V
authors.initials: VV
authors.lastname: Yanshole
authors.affiliation: International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk 630090, Russia.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Snytnikova
authors.affiliation: International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk 630090, Russia.
authors.forename: Alexey S
authors.initials: AS
authors.lastname: Kiryutin
authors.affiliation: International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk 630090, Russia.
authors.forename: Lyudmila V
authors.initials: LV
authors.lastname: Yanshole
authors.affiliation: International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk 630090, Russia.
authors.forename: Renad Z
authors.initials: RZ
authors.lastname: Sagdeev
authors.affiliation: International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk 630090, Russia; Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia. Electronic address: yura@tomo.nsc.ru.
authors.forename: Yuri P
authors.initials: YP
authors.lastname: Tsentalovich
mesh_terms: D000375:Aging / Q000378:metabolism; D019588:Aging, Premature / Q000378:metabolism; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005136:Eye Proteins / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D017208:Rats, Wistar
chemical_list: D005136:Eye Proteins
keywords: cataract; lens; metabolomics