title: Cationic methylcellulose derivative with serum-compatibility and endosome buffering ability for gene delivery systems.
abstract: In this work, methylcellulose was employed as a template polymer with graft of polyethylenimine 0.8 kDa (PEI0.8k) for gene delivery systems. Synthesized PEI-grafted oxidized methylcellulose (MC-PEI) could condense pDNA into positively charged and nano-sized particles, which could protect pDNA from serum nuclease. The cytotoxicity of MC-PEI was minimal in both serum-free and serum condition due to the biocompatibility of methylcellulose and low cytotoxicity of PEI0.8k. MC-PEI polyplex also showed low cytotoxicity in serum condition. In serum condition, MC-PEI showed less decreased transfection efficiency than PEI25k, meaning good serum-compatibility of MC-PEI. Bafilomycin A1-treated transfection results indicate that the transfection of MC-PEI is mediated via endosomal escape by endosome buffering ability. Flow cytometry results suggest that MC-PEI polyplex could be internalized into cells and efficiently deliver pDNA to cells due to its serum-compatibility. These results demonstrate that MC-PEI possesses a potential for efficient gene delivery systems.
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, Republic of Korea. Electronic address: st_kyunghwan@hanmail.net.
authors.forename: Kyunghwan
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, Republic of Korea. Electronic address: kitaefriend@snu.ac.kr.
authors.forename: Kitae
authors.initials: K
authors.lastname: Ryu
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, Republic of Korea; Research Institute of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, Republic of Korea. Electronic address: seal1004@snu.ac.kr.
authors.forename: Tae-il
authors.initials: TI
authors.lastname: Kim
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D002412:Cations / Q000737:chemistry / Q000633:toxicity; D002460:Cell Line; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008747:Methylcellulose / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity; D051379:Mice; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D011094:Polyethyleneimine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity; D044967:Serum / Q000378:metabolism; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D002412:Cations; D011094:Polyethyleneimine; D008747:Methylcellulose; D004247:DNA
keywords: Endosome buffering ability; Gene delivery systems; Low cytotoxicity; Low molecular weight PEI; Methylcellulose; Serum-compatibility