title: Comparative genetic mapping and genomic region collinearity analysis of the powdery mildew resistance gene Pm41.
abstract: By applying comparative genomics analyses, a high-density genetic linkage map narrowed the powdery mildew resistance gene Pm41 originating from wild emmer in a sub-centimorgan genetic interval. Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici, results in large yield losses worldwide. A high-density genetic linkage map of the powdery mildew resistance gene Pm41, originating from wild emmer (Triticum turgidum var. dicoccoides) and previously mapped to the distal region of chromosome 3BL bin 0.63-1.00, was constructed using an F5:6 recombinant inbred line population derived from a cross of durum wheat cultivar Langdon and wild emmer accession IW2. By applying comparative genomics analyses, 19 polymorphic sequence-tagged site markers were developed and integrated into the Pm41 genetic linkage map. Ultimately, Pm41 was mapped in a 0.6 cM genetic interval flanked by markers XWGGC1505 and XWGGC1507, which correspond to 11.7, 19.2, and 24.9 kb orthologous genomic regions in Brachypodium, rice, and sorghum, respectively. The XWGGC1506 marker co-segregated with Pm41 and could be served as a starting point for chromosome landing and map-based cloning as well as marker-assisted selection of Pm41. Detailed comparative genomics analysis of the markers flanking the Pm41 locus in wheat and the putative orthologous genes in Brachypodium, rice, and sorghum suggests that the gene order is highly conserved between rice and sorghum. However, intra-chromosome inversions and re-arrangements are evident in the wheat and Brachypodium genomic regions, and gene duplications are also present in the orthologous genomic regions of Pm41 in wheat, indicating that the Brachypodium gene model can provide more useful information for wheat marker development.
authors.affiliation: State Key Laboratory for Agrobiotechnology, Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Cui
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Chen
authors.forename: Deyun
authors.initials: D
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wu
authors.forename: Jingzhong
authors.initials: J
authors.lastname: Xie
authors.forename: Shuhong
authors.initials: S
authors.lastname: Ouyang
authors.forename: Delin
authors.initials: D
authors.lastname: Li
authors.forename: Yinlian
authors.initials: Y
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Lu
authors.forename: Guoxin
authors.initials: G
authors.lastname: Wang
authors.forename: Meihua
authors.initials: M
authors.lastname: Yu
authors.forename: Shenghui
authors.initials: S
authors.lastname: Zhou
authors.forename: Qixin
authors.initials: Q
authors.lastname: Sun
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D058431:Brachypodium / Q000235:genetics; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D020224:Expressed Sequence Tags; D020440:Gene Duplication / Q000235:genetics; D020131:Genes, Duplicate / Q000235:genetics; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics*; D023281:Genomics; D007178:Inbreeding; D012275:Oryza / Q000235:genetics; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D045868:Sorghum / Q000235:genetics; D014908:Triticum / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology
chemical_list: D005819:Genetic Markers