title: A mechanistic study on the anti-cancer activity of ethyl caffeate in human ovarian cancer SKOV-3 cells.
abstract: In the present study, we investigated the effect and molecular mechanism of ethyl caffeate (EC), a natural phenolic compound isolated from Ligularia fischeri, on human ovarian cancer cell proliferation and progression. EC-mediated inhibition of cell proliferation in SKOV-3 cells was accompanied by reduced expression of cell cycle-related proteins such as cyclin-dependent kinases and cyclins, resulting in pRb hypophosphorylation and G₁ phase cell cycle arrest. Moreover, EC treatment markedly inhibited cell migration and invasion. These regulatory effects of EC on ovarian cancer cell proliferation, migration and invasion were associated with inactivation of mitogenic signaling pathways such as Akt, ERK and p38(MAPK), and down-regulation of cell surface signaling molecules including receptor tyrosine kinases, integrin α3β1 and N-cadherin. Taken together, these findings suggest further evaluation and development of EC for the treatment and prevention of ovarian cancer.
authors.affiliation: College of Pharmacy, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Ha Neul
authors.initials: HN
authors.lastname: Lee
authors.affiliation: Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion, Suwon 443-270, Republic of Korea.
authors.forename: Jin-Kyu
authors.initials: JK
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Jae Hyeon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion, Suwon 443-270, Republic of Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Ahn
authors.affiliation: College of Pharmacy, Dankook University, Cheonan 330-714, Republic of Korea; Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion, Suwon 443-270, Republic of Korea. Electronic address: jsoh@gstep.re.kr.
authors.forename: Joa Sub
authors.initials: JS
authors.lastname: Oh
authors.affiliation: College of Pharmacy, Dankook University, Cheonan 330-714, Republic of Korea. Electronic address: dwseomb@dankook.ac.kr.
authors.forename: Dong-Wan
authors.initials: DW
authors.lastname: Seo
mesh_terms: D015153:Blotting, Western; D002109:Caffeic Acids / Q000494:pharmacology* / Q000627:therapeutic use; D002453:Cell Cycle / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012334:RNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D016160:Retinoblastoma Protein / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D002109:Caffeic Acids; D016213:Cyclins; D012334:RNA, Neoplasm; D016160:Retinoblastoma Protein; C032773:ethyl caffeate; D051057:Proto-Oncogene Proteins c-akt; D018844:Cyclin-Dependent Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Ethyl caffeate; Integrin α3β1; Ligularia fischeri; Ovarian cancer; Receptor tyrosine kinase