title: [Concordance of two serological tests for the diagnosis of Chagas disease].
abstract: OBJECTIVE
  Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates.
  
  
  METHODS
  A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings.
  
  
  RESULTS
  The in-house ELISA it was an agreement of 0.99 (95 % CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 % were positive by ELISA and 10.9 % by IIF.
  
  
  CONCLUSIONS
  The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas.
authors.affiliation: Centro de Investigaciones en Enfermedades Tropicales -CINTROP-, Universidad Industrial de Santander, Colombia.
authors.forename: Ana E
authors.initials: AE
authors.lastname: Farfán-García
authors.affiliation: Centro de Investigaciones en Enfermedades Tropicales -CINTROP-, Universidad Industrial de Santander, Colombia.
authors.forename: Yeny Z
authors.initials: YZ
authors.lastname: Castellanos-Domínguez
authors.affiliation: Centro de Investigaciones en Enfermedades Tropicales -CINTROP-, Universidad Industrial de Santander, Colombia.
authors.forename: Katherine P
authors.initials: KP
authors.lastname: Luna-Marín
authors.affiliation: Centro de Investigaciones en Enfermedades Tropicales -CINTROP-, Universidad Industrial de Santander, Colombia.
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Angulo-Silva
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014355:Chagas Disease / Q000097:blood* / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007223:Infant; D008875:Middle Aged; D012698:Serologic Tests; D055815:Young Adult