title: Determination and pharmacokinetics of linarin in rat plasma after intramuscular administration of linarin solution and Yejuhua injection by HPLC.
abstract: A simple and sensitive HPLC method was developed and validated for the determination of linarin in rat plasma. Plasma samples were carried out by protein precipitation with acetonitrile and separated on a Shim-pack VP-ODS analytical column (250 × 4.6 mm, 5 µm). The calibration curve was linear in the measured range of 20.8-4160 ng/mL and the lower limit of quantification was 20.8 ng/mL for linarin. The validated method was successfully applied to the pharmacokinetic study of linarin in rat plasma after intramuscular administration of monomeric compound and traditional Chinese medicinal preparation - Yejuhua injection.
authors.affiliation: Shenyang Key Laboratory of Active Components of Chinese Medicine Screening and Evaluation, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, 110016, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Mao
authors.forename: Xuan
authors.initials: X
authors.lastname: Xu
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Li
authors.forename: Kaishun
authors.initials: K
authors.lastname: Bi
authors.forename: Ying
authors.initials: Y
authors.lastname: Jia
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006027:Glycosides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D007273:Injections, Intramuscular; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006027:Glycosides; C008282:linarin
keywords: Yejuhua injection; linarin; pharmacokinetics