title: Label-free logic modules and two-layer cascade based on stem-loop probes containing a G-quadruplex domain.
abstract: A simple, versatile, and label-free DNA computing strategy was designed by using toehold-mediated strand displacement and stem-loop probes. A full set of logic gates (YES, NOT, OR, NAND, AND, INHIBIT, NOR, XOR, XNOR) and a two-layer logic cascade were constructed. The probes contain a G-quadruplex domain, which was blocked or unfolded through inputs initiating strand displacement and the obviously distinguishable light-up fluorescent signal of G-quadruplex/NMM complex was used as the output readout. The inputs are the disease-specific nucleotide sequences with potential for clinic diagnosis. The developed versatile computing system based on our label-free and modular strategy might be adapted in multi-target diagnosis through DNA hybridization and aptamer-target interaction.
authors.affiliation: Suzhou Key Laboratory of Nanotheranostics, Division of Nanobiomedicine, Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123 (P.R. China); Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry & Molecular Sciences, Wuhan University, Wuhan, 430072 (P.R. China).
authors.forename: Yahui
authors.initials: Y
authors.lastname: Guo
authors.forename: Junjie
authors.initials: J
authors.lastname: Cheng
authors.forename: Jine
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhou
authors.forename: Jiming
authors.initials: J
authors.lastname: Hu
authors.forename: Renjun
authors.initials: R
authors.lastname: Pei
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D054856:G-Quadruplexes; D008128:Logic
chemical_list: D052157:Aptamers, Nucleotide; D015342:DNA Probes
keywords: DNA computing; G quadruplexes; label-free detection; logic gates; multiple-input cascade