title: Microparticles and sudden cardiac death due to coronary occlusion. The TIDE (Thrombus and Inflammation in sudden DEath) study.
abstract: OBJECTIVE
  The pattern of coronary occlusion might contribute to the onset of ventricular arrhythmia and sudden cardiac death (SCD). We hypothesized that the concentrations of microparticles might differ between SCD and ST-elevation myocardial infarction (STEMI) patients without rhythmic disturbances.
  
  
  RESULTS
  The study sample includes consecutive patients hospitalized in two French tertiary centres between 2006 and 2011 for SCD with angiographically-proven acute coronary occlusion (n=23), for STEMI (n=61) and for a planned percutaneous coronary angioplasty (PCI) (n=35, controls). During PCI blood was collected in the arch of aorta (systemic blood) before and after the procedure and in the culprit coronary lesion with an aspiration catheter. Microparticles were analysed by flow cytometry in a blinded manner to quantify endothelial (CD144+), platelet (CD41+), leucocyte (CD11a+) and erythrocyte (CD235a+) derived microparticles. After multivariate analysis, intracoronary concentrations of endothelial-derived microparticles were significantly higher in SCD than in STEMI patients (129 (74-185) vs. 50 (21-118) nb/µl; p < 0.01). Intracoronary and systemic blood concentrations of platelet-derived microparticles were not different between SCD and controls, suggesting limited impact of cardiac massage and electric defibrillation in microparticle concentrations.
  
  
  CONCLUSIONS
  The higher concentrations of endothelial-derived microparticles in SCD due to acute coronary occlusion compared with STEMI without rhythmic disturbances suggests different patterns of acute coronary occlusion.
authors.affiliation: Paris Cardiovascular Research Centre (PARCC), INSERM UMRS 970, Sorbonne Paris Cité, Paris France Paris Sudden Death Expertise Centre, France jean-philippe.empana@inserm.fr xavier.jouven@inserm.fr.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Empana
authors.affiliation: Paris Cardiovascular Research Centre (PARCC), INSERM UMRS 970, Sorbonne Paris Cité, Paris France.
authors.forename: Chantal M
authors.initials: CM
authors.lastname: Boulanger
authors.affiliation: Paris Cardiovascular Research Centre (PARCC), INSERM UMRS 970, Sorbonne Paris Cité, Paris France Paris Sudden Death Expertise Centre, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Tafflet
authors.affiliation: Paris Cardiovascular Research Centre (PARCC), INSERM UMRS 970, Sorbonne Paris Cité, Paris France.
authors.forename: Jean M
authors.initials: JM
authors.lastname: Renard
authors.affiliation: Paris Cardiovascular Research Centre (PARCC), INSERM UMRS 970, Sorbonne Paris Cité, Paris France.
authors.forename: Aurelie S
authors.initials: AS
authors.lastname: Leroyer
authors.affiliation: Assistance Publique des Hôpitaux de Paris (APHP), Cochin University Hospital, Department of Intensive Cardiology, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Varenne
authors.affiliation: Paris Cardiovascular Research Centre (PARCC), INSERM UMRS 970, Sorbonne Paris Cité, Paris France Paris Sudden Death Expertise Centre, France.
authors.forename: Christof
authors.initials: C
authors.lastname: Prugger
authors.affiliation: Assistance Publique des Hôpitaux de Paris (APHP), Institut de Cardiologie, La Pitié Salpetriere University Hospital, Department of Intensive Cardiology; Paris 6 University, France.
authors.forename: Johanne
authors.initials: J
authors.lastname: Silvain
authors.affiliation: Paris Cardiovascular Research Centre (PARCC), INSERM UMRS 970, Sorbonne Paris Cité, Paris France.
authors.forename: Alain
authors.initials: A
authors.lastname: Tedgui
authors.affiliation: Paris Cardiovascular Research Centre (PARCC), INSERM UMRS 970, Sorbonne Paris Cité, Paris France Paris Sudden Death Expertise Centre, France Assistance Publique des Hôpitaux de Paris (APHP), Cochin University Hospital, Department of Intensive Care, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Cariou
authors.affiliation: Assistance Publique des Hôpitaux de Paris (APHP), Institut de Cardiologie, La Pitié Salpetriere University Hospital, Department of Intensive Cardiology; Paris 6 University, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Montalescot
authors.affiliation: Paris Cardiovascular Research Centre (PARCC), INSERM UMRS 970, Sorbonne Paris Cité, Paris France Paris Sudden Death Expertise Centre, France Assistance Publique des Hôpitaux de Paris (APHP), Georges Pompidou European Hospital, Department of Cardiology, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Jouven
authors.affiliation: Paris Cardiovascular Research Centre (PARCC), INSERM UMRS 970, Sorbonne Paris Cité, Paris France Paris Sudden Death Expertise Centre, France Assistance Publique des Hôpitaux de Paris (APHP), Georges Pompidou European Hospital, Department of Cardiology, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Spaulding
mesh_terms: D000368:Aged; D055252:Cell-Derived Microparticles / Q000473:pathology*; D054059:Coronary Occlusion / Q000150:complications* / Q000473:pathology; D003328:Coronary Thrombosis / Q000209:etiology* / Q000473:pathology; D016757:Death, Sudden, Cardiac / Q000209:etiology* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000209:etiology / Q000473:pathology; D011446:Prospective Studies
keywords: Sudden cardiac death; endothelium; microparticles; myocardial infarction; preconditioning