title: Hypolipidemic activity of Taraxacum mongolicum associated with the activation of AMP-activated protein kinase in human HepG2 cells.
abstract: This study investigated the hypolipidemic effect and potential mechanisms of T. mongolicum extracts. T. mongolicum was extracted by refluxing three times with water (TM-1), 50% ethanol (TM-2) and 95% ethanol (TM-3). TM-2 contained components with the most effective hypolipidemic potentials in HepG2 cells. Extended administration of TM-2 stimulated a significant reduction in body weight and levels of serum triglyceride LDL-C and total cholesterol in rats. To evaluate the bioactive compounds, we successively fractionated TM-2 with n-hexane (TM-4), dichloromethane (TM-5), ethyl acetate (TM-6), and water (TM-7). TM-4 fraction had the most effective hypolipidemic potential in HepG2 cells, and it decreased the expression of fatty acid synthase (FASN) and inhibited the activity of acetyl-coenzyme A carboxylase (ACC) through the phosphorylation of AMP-activated protein kinase (AMPK). Linoleic acid, phytol and tetracosanol are bioactive compounds identified from TM-4. These results suggest that T. mongolicum is expected to be useful for hypolipidemic effects.
authors.affiliation: Graduate Institute of Pharmaceutical Chemistry, College of Pharmacy, China Medical University, No.91, Hsueh-Shih Road, Taichung, 40402, Taiwan. ljhuang@mail.cmu.edu.tw.
authors.forename: Yan-Jin
authors.initials: YJ
authors.lastname: Liu
authors.forename: Po-Chuen
authors.initials: PC
authors.lastname: Shieh
authors.forename: Jang-Chang
authors.initials: JC
authors.lastname: Lee
authors.forename: Fu-An
authors.initials: FA
authors.lastname: Chen
authors.forename: Chih-Hung
authors.initials: CH
authors.lastname: Lee
authors.forename: Sheng-Chu
authors.initials: SC
authors.lastname: Kuo
authors.forename: Chi-Tang
authors.initials: CT
authors.lastname: Ho
authors.forename: Daih-Huang
authors.initials: DH
authors.lastname: Kuo
authors.forename: Li-Jiau
authors.initials: LJ
authors.lastname: Huang
authors.forename: Tzong-Der
authors.initials: TD
authors.lastname: Way
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D056945:Hep G2 Cells; D006801:Humans; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D031205:Taraxacum / Q000737:chemistry*; D014280:Triglycerides / Q000097:blood
chemical_list: D019440:Anti-Obesity Agents; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D000960:Hypolipidemic Agents; D010936:Plant Extracts; D014280:Triglycerides; D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase