title: Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles.
abstract: Second generation sequencing (SGS) may revolutionize the field of forensic STR typing. Two of the essential requirements for implementation of an SGS based approach for forensic investigations are (1) establishment of adequate frequency databases and (2) adoption of a new STR nomenclature. We report the STR sequences and allele frequencies of three STR loci: D3S1358, D12S391 and D21S11 in 197 unrelated Danes. We used a new STR nomenclature that depicts the locus name used in forensic genetics, the length of the repeat region divided by the repeat length (typically 4 nucleotides) and detailed sequence information of possible sub-repeats and SNPs within the amplified fragment.
authors.affiliation: Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark; Faculty of Mathematical, Physical and Natural Sciences, University of Palermo, Palermo, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Gelardi
authors.affiliation: Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark. Electronic address: eszter.rockenbauer@sund.ku.dk.
authors.forename: Eszter
authors.initials: E
authors.lastname: Rockenbauer
authors.affiliation: Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
authors.forename: Sigrun
authors.initials: S
authors.lastname: Dalsgaard
authors.affiliation: Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
authors.forename: Claus
authors.initials: C
authors.lastname: Børsting
authors.affiliation: Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
authors.forename: Niels
authors.initials: N
authors.lastname: Morling
mesh_terms: D003718:Denmark; D005787:Gene Frequency; D006801:Humans; D018895:Microsatellite Repeats; D017422:Sequence Analysis, DNA / Q000379:methods*; D009626:Terminology as Topic
keywords: Forensic genetics; Frequency database; Nomenclature; STR; Second generation sequencing