title: Insights into the structure and architecture of the CCR4-NOT complex.
abstract: The CCR4-NOT complex is a highly conserved, multifunctional machinery with a general role in controlling mRNA metabolism. It has been implicated in a number of different aspects of mRNA and protein expression, including mRNA degradation, transcription initiation and elongation, ubiquitination, and protein modification. The core CCR4-NOT complex is evolutionarily conserved and consists of at least three NOT proteins and two catalytic subunits. The L-shaped complex is characterized by two functional modules bound to the CNOT1/Not1 scaffold protein: the deadenylase or nuclease module containing two enzymes required for deadenylation, and the NOT module. In this review, we will summarize the currently available information regarding the three-dimensional structure and assembly of the CCR4-NOT complex, in order to provide insight into its roles in mRNA degradation and other biological processes.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University Tianjin, China ; College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University Tianjin, China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University Tianjin, China ; College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Aili
authors.initials: A
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Qionglin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University Tianjin, China ; College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Mark G
authors.initials: MG
authors.lastname: Bartlam
keywords: CBR4–NOT complex; NOT module; deadenylation; mRNA decay; poly(A); protein structure