title: Control of pro-inflammatory cytokine release from human monocytes with the use of an interleukin-10 monoclonal antibody.
abstract: The monocytes (MONOs) can be considered as "double-edge swords"; they have both important pro-inflammatory and anti-inflammatory functions manifested in part by cytokine production and release. Although MONOs are circulating cells, they are the major precursors of a variety of tissue-specific immune cells such as the alveolar macrophage, dendritic cells, microglial cells, and Kupffer cells. Unlike the polymorphonuclear leukocyte, which produces no or very little interleukin-10 (IL-10), the monocyte can produce this potent anti-inflammatory cytokine to control inflammation. IL-10, on an equimolar basis, is a more potent inhibitor of pro-inflammatory cytokines produced by monocytes than many anti-inflammatory glucocorticoids which are used clinically. This chapter describes how to isolate monocytes from human blood and the use of IL-10 monoclonal antibody to determine the effect and timing of endogenous IL-10 release on the production and release of pro-inflammatory cytokines.
authors.affiliation: The Feinstein Institute for Medical Research, Manhasset, NY, 11030, USA.
authors.forename: Hardik
authors.initials: H
authors.lastname: Patel
authors.forename: Dennis
authors.initials: D
authors.lastname: Davidson
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D018189:Immunomagnetic Separation; D016753:Interleukin-10 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015850:Interleukin-6 / Q000032:analysis* / Q000378:metabolism; D016209:Interleukin-8 / Q000032:analysis* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D016753:Interleukin-10