title: Visualized analysis of cellular fatty acid profiles of Vibrio parahaemolyticus strains under cold stress.
abstract: Vibrio parahaemolyticus is a common foodborne bacterial pathogen, which survives in cold environments and is sometimes difficult to culture. Fatty acid analysis under cold stress was conducted for several V. parahaemolyticus strains using gas chromatography/mass spectrometry, and the results were compared with those of the controls. All the fatty acid profiles obtained were visualized by multidimensional scaling (MDS) and self-organized map (SOM). It was observed that the fatty acid profiles of V. parahaemolyticus substantially changed under cold stress. The percentage of methyl palmitate remarkably decreased and that of methyl palmitoleate (except for two strains) and methyl oleate increased. These findings demonstrate the role of fatty acids in cold stress. The changes in the fatty acid profiles illustrated by MDS and SOM could differentiate strains under cold stress from the controls and can potentially lead to a method of detecting injured cold-stressed V. parahaemolyticus.
authors.affiliation: Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao, China.
authors.forename: Juntao
authors.initials: J
authors.lastname: Jia
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
authors.forename: Lijun
authors.initials: L
authors.lastname: Yang
authors.forename: Chengzhu
authors.initials: C
authors.lastname: Liang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jia
authors.forename: Liqing
authors.initials: L
authors.lastname: Zhao
mesh_terms: D003080:Cold Temperature; D005227:Fatty Acids / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism; D005516:Food Microbiology / Q000379:methods; D009829:Oleic Acids / Q000378:metabolism; D010168:Palmitates / Q000378:metabolism; D013312:Stress, Physiological / Q000502:physiology*; D014736:Vibrio parahaemolyticus / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D009829:Oleic Acids; D010168:Palmitates; C068012:methyl palmitoleate; C005576:methyl oleate; C019012:methyl palmitate
keywords: Vibrio parahaemolyticus; cold stress; fatty acids; multidimensional scaling; self-organized map