title: Expression of latent membrane proteins in Epstein‑Barr virus-transformed lymphocytes in vitro.
abstract: Infection with Epstein-Barr virus (EBV) induces activation and proliferation of B lymphocytes. Detection of latent membrane protein (LMP)-1 is used to identify the proliferative ability of B cells. However, changes in the expression levels of the three LMPs during EBV-induced B lymphocyte transformation, have not yet been reported. In the present study, the expression levels of LMP-1, LMP-2A and LMP-2B were compared between EBV-transformed B lymphocytes and paired normal lymphocytes. Seven lymphoblast cell lines were established by EBV infection of normal human lymphocytes in vitro. The expression levels of LMP genes and LMP-1 protein were determined using quantitative (q)PCR and western blotting in lymphoblasts and normal lymphocytes, respectively. The expression of LMP1, LMP-2A and LMP-2B genes was significantly upregulated in EBV-induced lymphoblasts compared with the normal lymphocytes. The LMP-1 protein level was also significantly increased in EBV-transformed B lymphocytes. Expression of LMP1, LMP-2A and LMP-2B genes was significantly upregulated in EBV-induced lymphoblasts, suggesting LMP genes are important in the transformation of human lymphocytes.
authors.affiliation: Cancer Research Institute, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Yunlian
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Cancer Research Institute, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Luo
authors.affiliation: Cancer Research Institute, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Ailan
authors.initials: A
authors.lastname: Cheng
authors.affiliation: Cancer Research Institute, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Suli
authors.initials: S
authors.lastname: Lu
authors.affiliation: Cancer Research Institute, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Xu
authors.affiliation: Cancer Research Institute, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Fu
authors.affiliation: Cancer Research Institute, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Runliang
authors.initials: R
authors.lastname: Gan
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000821:virology*; D002461:Cell Line, Transformed; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D015854:Up-Regulation; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins