title: Mycobacteriophage L5Gp56, a novel member of the NrdH family of redoxins.
abstract: Mycobacteriophage L5 gene 56 encodes a putative thioredoxin family protein. Phylogenetic analysis revealed that Gp56 and related proteins are distantly related to NrdH - a glutaredoxin homolog which has thioredoxin-like properties. To understand its function, the recombinant version of the protein was biochemically characterized. For the sake of comparison, a mycobacterial thioredoxin, TrxB, was included in the study. Results show that Gp56 can be reduced by dithiothreitol, but only at a higher concentration as compared with TrxB, indicating that the standard redox potential of Gp56 is lower than that of TrxB. The reduced protein can subsequently act as a reductant of protein disulfide bonds. Gp56 can be reduced by NADPH with the help of thioredoxin reductase (TrxR) but less efficiently as compared with TrxB. The abilities of Gp56 and TrxB to reduce Gp50, the L5-encoded ribonucleotide reductase, was examined. While both are capable of executing this function, the former needs more reducing equivalents in the process as compared with the latter. This study shows that L5Gp56 represents a new class of NrdH-like proteins that function optimally in a reducing environment.
authors.affiliation: Department of Microbiology, Bose Institute, Kolkata, India.
authors.forename: Prithwiraj
authors.initials: P
authors.lastname: Kirtania
authors.forename: Bidisha
authors.initials: B
authors.lastname: Bhattacharya
authors.forename: Sujoy K
authors.initials: SK
authors.lastname: Das Gupta
mesh_terms: D054477:Glutaredoxins / Q000235:genetics; D009160:Mycobacteriophages / Q000235:genetics*; D009249:NADP / Q000235:genetics; D010802:Phylogeny; D012264:Ribonucleotide Reductases / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013880:Thioredoxin-Disulfide Reductase / Q000235:genetics; D013879:Thioredoxins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D054477:Glutaredoxins; D014764:Viral Proteins; D013879:Thioredoxins; D009249:NADP; D012264:Ribonucleotide Reductases; D013880:Thioredoxin-Disulfide Reductase
keywords: NrdH; mycobacteriophage; ribonucleotide reductase; thioredoxin