title: Rapid and multiplex microRNA detection on graphically encoded silica suspension array.
abstract: MicroRNA (miRNA), an 18-24-nucleotide noncoding RNA molecule, has become an ideal class of biomarker candidates for clinical diagnosis of cancers. By now, a number of detection methods for miRNAs have been developed on planar arrays and suspension arrays. In this work, we describe a hybridization-triggered fluorescence strategy for label-free and multiplex miRNA detection on graphically encoded silica suspension array. The total RNA is directly applied for analysis with an 8-mer Universal Tag which can be selectively captured by the capture probe via base-stacking effects. Benefiting from base-stacking effects, this novel method exhibits superb discrimination ability toward the 5' and 3' end single-nucleotide alteration. Mature miRNAs can be distinguished from their corresponding pre-miRNAs easily. Moreover, the estimated detection limit of 5 amol is comparable to some of the most sensitive methods. All these mentioned characteristics offer exciting possibilities for discovery and clinical applications.
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
authors.forename: Kexiao
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China. Electronic address: jli2006@sinano.ac.cn.
authors.forename: Jiong
authors.initials: J
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D020869:Gene Expression Profiling / Q000191:economics / Q000295:instrumentation; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D020411:Oligonucleotide Array Sequence Analysis / Q000191:economics / Q000295:instrumentation*; D012822:Silicon Dioxide / Q000737:chemistry*; D013535:Suspensions; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D013535:Suspensions; D012822:Silicon Dioxide
keywords: Base stacking; Graphical encoding; MicroRNA; Suspension array