title: Effects of sodium selenite on aflatoxin B1-induced decrease of ileal IgA+ cell numbers and immunoglobulin contents in broilers.
abstract: This study was aimed to assess the protective effect of sodium selenite on the ileum mucosal immunologic injury induced by AFB1. One hundred eighty-one-day-old healthy male Avian broilers were divided into four groups of three replicates and 15 birds per replicate and fed with basal diet (control group), 0.3 mg/kg AFB1 (AFB1 group), 0.4 mg/kg Se (+Se group), and 0.3 mg/kg AFB1 + 0.4 mg/kg Se (AFB1 + Se group) respectively. The numbers of IgA(+) cells of ileum were determined by immunohistochemistry as well as the contents of sIgA, IgA, IgG, and IgM in the mucosa of ileum by ELISA. Compared with those in the control group, the numbers of IgA(+) cells as well as the sIgA, IgA, IgG, and IgM contents were decreased in the AFB1 group. However, compared with those in the AFB1 group, the numbers of IgA(+) cells as well as the sIgA, IgA, IgG, and IgM contents were increased in the AFB1 + Se group, and these data had no difference between AFB1 + Se group and control group. It was concluded that 0.3 mg/kg AFB1 could reduce the humoral immune function of the ileum mucosa, but 0.4 mg/kg supplemented dietary selenium could protect the mucosal humoral immune function from AFB1-induced impairment.
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, 625014, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: He
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Geng
authors.forename: Weimin
authors.initials: W
authors.lastname: Lai
authors.forename: Gang
authors.initials: G
authors.lastname: Shu
authors.forename: Li
authors.initials: L
authors.lastname: Tang
mesh_terms: D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000818:Animals; D002452:Cell Count; D002645:Chickens / Q000378:metabolism*; D004032:Diet; D019587:Dietary Supplements; D004797:Enzyme-Linked Immunosorbent Assay; D007082:Ileum / Q000187:drug effects / Q000276:immunology* / Q000473:pathology*; D007070:Immunoglobulin A / Q000032:analysis*; D007136:Immunoglobulins / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008297:Male; D018038:Sodium Selenite / Q000494:pharmacology*
chemical_list: D007070:Immunoglobulin A; D007136:Immunoglobulins; D016604:Aflatoxin B1; D018038:Sodium Selenite