title: Morphology, structure and function characterization of PEI modified magnetic nanoparticles gene delivery system.
abstract: Modified magnetic nanoparticles are used as non-viral gene carriers in biological applications. To achieve successful gene delivery, it is critical that nanoparticles effectually assemble with nucleic acids. However, relatively little work has been conducted on the assemble mechanisms between nanoparticles and DNA, and its effects on transfection efficiency. Using biophysical and biochemical characterization, along with Atomic force microscopy (AFM) and Transmission electron microscopy (TEM), we investigate the morphologies, assembling structures and gene delivering abilities of the PEI modified magnetic nanoparticles (MNPs) gene delivery system. In this gene delivery system, MNP/DNA complexes are formed via binding of DNA onto the surface of MNPs. MNPs are favorable to not only increase DNA concentration but also prevent DNA degradation. Magnetofection experiments showed that MNPs has low cytotoxicity and introduces highly stable transfection in mammalian somatic cells. In addition, different binding ratios between MNPs and DNA result in various morphologies of MNP/DNA complexes and have an influence on transfection efficiency. Dose-response profile indicated that transfection efficiency positively correlate with MNP/DNA ratio. Furthermore, intracellular tracking demonstrate that MNPs move though the cell membranes, deliver and release exogenous DNA into the nucleus.
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China; Nano biological Research Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China; Nano biological Research Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Haixin
authors.initials: H
authors.lastname: Cui
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Chen
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Cui
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Changjiao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Meng
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D049452:Green Fluorescent Proteins / Q000235:genetics; D042541:Intracellular Space / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010957:Plasmids / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry* / Q000633:toxicity; D013499:Surface Properties
chemical_list: D004337:Drug Carriers; D058185:Magnetite Nanoparticles; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D004247:DNA