title: Oral dosing in adult zebrafish: proof-of-concept using pharmacokinetics and pharmacological evaluation of carbamazepine.
abstract: METHODS
  We describe a method for obtaining pharmacokinetics (PK) and pharmacology data from adult zebrafish in terms of mg/kg using a novel method of oral administration. Using carbamazepine (CBZ) as a test drug, we employed dried blood spot (DBS) cards to enable drug quantification for PK; and we evaluated the pharmacological anxiolytic effect using novel tank test.
  
  
  RESULTS
  The PK study confirmed the presence of CBZ in both blood and brain and the behavioural study showed dose dependent anxiolytic effect. The reproducibility of oral dosing was confirmed by the fact that the results obtained in both the experiments had negligible errors.
  
  
  CONCLUSIONS
  This report enables a novel approach for optimizing the utility of zebrafish in drug discovery and drug delivery research.
authors.affiliation: Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India. Electronic address: pushkark@drils.org.
authors.forename: Pushkar
authors.initials: P
authors.lastname: Kulkarni
authors.affiliation: Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India.
authors.forename: Girish Hari
authors.initials: GH
authors.lastname: Chaudhari
authors.affiliation: Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Cosmic Discoveries, (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India.
authors.forename: Vijaykumar
authors.initials: V
authors.lastname: Sripuram
authors.affiliation: Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India.
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Banote
authors.affiliation: Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India.
authors.forename: Krishna Tulasi
authors.initials: KT
authors.lastname: Kirla
authors.affiliation: Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India.
authors.forename: Razia
authors.initials: R
authors.lastname: Sultana
authors.affiliation: Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Cosmic Discoveries, (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Rao
authors.affiliation: Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Cosmic Discoveries, (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India. Electronic address: soruganti@drils.org.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Oruganti
authors.affiliation: Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India. Electronic address: kiranamc@drils.org.
authors.forename: Kiranam
authors.initials: K
authors.lastname: Chatti
mesh_terms: D000284:Administration, Oral; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D001812:Blood-Brain Barrier; D002220:Carbamazepine / Q000493:pharmacokinetics* / Q000494:pharmacology; D008297:Male; D015027:Zebrafish
chemical_list: D014151:Anti-Anxiety Agents; D002220:Carbamazepine
keywords: Drug delivery; Drug discovery; Oral administration; Pharmacokinetics; Zebrafish