title: Advanced mass spectrometry-based multi-omics technologies for exploring the pathogenesis of hepatocellular carcinoma.
abstract: Hepatocellular carcinoma (HCC) is one of the primary hepatic malignancies and is the third most common cause of cancer related death worldwide. Although a wealth of knowledge has been gained concerning the initiation and progression of HCC over the last half century, efforts to improve our understanding of its pathogenesis at a molecular level are still greatly needed, to enable clinicians to enhance the standards of the current diagnosis and treatment of HCC. In the post-genome era, advanced mass spectrometry driven multi-omics technologies (e.g., profiling of DNA damage adducts, RNA modification profiling, proteomics, and metabolomics) stand at the interface between chemistry and biology, and have yielded valuable outcomes from the study of a diversity of complicated diseases. Particularly, these technologies are being broadly used to dissect various biological aspects of HCC with the purpose of biomarker discovery, interrogating pathogenesis as well as for therapeutic discovery. This proof of knowledge-based critical review aims at exploring the selected applications of those defined omics technologies in the HCC niche with an emphasis on translational applications driven by advanced mass spectrometry, toward the specific clinical use for HCC patients. This approach will enable the biomedical community, through both basic research and the clinical sciences, to enhance the applicability of mass spectrometry-based omics technologies in dissecting the pathogenesis of HCC and could lead to novel therapeutic discoveries for HCC.
authors.affiliation: Chongqing University Innovative Drug Research Centre, School of Chemistry and Chemical Engineering, Chongqing, 401331, PR China.
authors.forename: Wenna
authors.initials: W
authors.lastname: Nie
authors.affiliation: Chongqing University Innovative Drug Research Centre, School of Chemistry and Chemical Engineering, Chongqing, 401331, PR China.
authors.forename: Leyu
authors.initials: L
authors.lastname: Yan
authors.affiliation: Interdisciplinary Research Group in Infectious Diseases, Singapore-MIT Alliance for Research & Technology, Singapore, 138602, Singapore.
authors.forename: Yie H
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Radiation Oncology, Montefiore Medical Center, New York, New York, 10461.|Department of Medicine, Albert Einstein College of Medicine, New York, New York, 10461.
authors.forename: Chandan
authors.initials: C
authors.lastname: Guha
authors.affiliation: Stable Isotope and Metabolomics Core Facility, Diabetes Research and Training Center, Department of Medicine, Albert Einstein College of Medicine, New York, New York, 10461.
authors.forename: Irwin J
authors.initials: IJ
authors.lastname: Kurland
authors.affiliation: Chongqing University Innovative Drug Research Centre, School of Chemistry and Chemical Engineering, Chongqing, 401331, PR China.|Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD, 4059, Australia.
authors.forename: Haitao
authors.initials: H
authors.lastname: Lu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D018736:DNA Adducts / Q000032:analysis / Q000378:metabolism; D004249:DNA Damage; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D040901:Proteomics / Q000379:methods; D012313:RNA / Q000737:chemistry / Q000378:metabolism
chemical_list: D018736:DNA Adducts; D012313:RNA
keywords: DNA damage adducts; RNA modifications; hepatocellular carcinoma; metabolomics; omics technology; proteomics