title: Edaravone protect against retinal damage in streptozotocin-induced diabetic mice.
abstract: Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one), a free radical scavenger, is used for the clinical treatment of retinal injury. In this study, we investigated the protective effects of edaravone against diabetic retinal damage in the mouse. Diabetic retinopathy in the mouse was induced by injection of streptozotocin. Edaravone was given once-daily and was intraperitoneally (i.p.) treated at a dose of 3 mg/kg from streptozotocin injection to 4 weeks after onset of diabetes. Retinal ganglion cells (RGCs) damage was evaluated by recording the pattern electroretinogram (ERG). RGCs damage was also detected by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, and the levels of reactive oxygen species (ROS) were determined fluorometrically. The expressions of phosporylated-ERK1/2, BDNF, and caspase-3 were determined by Western blot analysis. Retinal levels of ROS, phosphorylated ERK1/2, and cleaved caspase-3 were significantly increased, whereas the expression of BDNF was significantly decreased in the retinas of diabetic mice, compared to nondiabetic mice. Administration of edaravone significantly attenuated diabetes induced RGCs death, upregulation of ROS, ERK1/2 phosphorylation, and cleaved caspase-3 and downregulation of BDNF. These findings suggest that oxidative stress plays a pivotal role in diabetic retinal damage and that systemic administration of edaravone may slow the progression of retinal neuropathy induced by diabetes.
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
authors.forename: Dongqing
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
authors.forename: Yidan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Hang
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
authors.forename: Songtao
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
authors.forename: Qinghuai
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000473:pathology; D003930:Diabetic Retinopathy; D000077553:Edaravone; D004596:Electroretinography; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D012164:Retinal Diseases / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D000077553:Edaravone; D000983:Antipyrine