title: Low medium pH value enhances anthocyanin accumulation in Malus crabapple leaves.
abstract: Anthocyanin is a critical factor involved in coloration of plant tissues, but the mechanism how medium pH values affect anthocyanin accumulation in woody plants is unknown. We analyzed anthocyanin composition and the expression of elements encoding anthocyanin and flavonols biosynthesis underlying different medium pH values by using three different leave color type cultivars. HPLC analysis demonstrated that high medium pH values treatment induced a dramatic decrease in the concentration of cyaniding in crabapple leaves. Conversely, the high medium pH values induced up-regulation of the content of flavones and flavonols, suggesting that low pH treatment-induced anthocyanin accumulation. Quantitative real time PCR experiment showed the expression level of anthocyanidin synthase (McANS) and uridine diphosphate glucose flavonoid 3-O-glucosyltransferase (McUFGT) was up-regulated by low pH values treatment, and high medium pH value treatment up-regulate the transcription level of flavonol synthase (McFLS). Meanwhile, several MYB TFs have been suggested in the regulation of pH responses. These results strongly indicate that the low pH treatment-induced anthocyanin accumulation is mediated by the variation of mRNA transcription of the anthocyanin biosynthetic genes.
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
authors.forename: Yanchen
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China; Key Laboratory of New Technology in Agricultural Application of Beijing, Beijing University of Agriculture, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China; Key Laboratory of New Technology in Agricultural Application of Beijing, Beijing University of Agriculture, Beijing, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Song
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Li
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China; Key Laboratory of New Technology in Agricultural Application of Beijing, Beijing University of Agriculture, Beijing, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Tian
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
authors.forename: Kaina
authors.initials: K
authors.lastname: Jin
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China; Key Laboratory of New Technology in Agricultural Application of Beijing, Beijing University of Agriculture, Beijing, China.
authors.forename: Yuncong
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000872:Anthocyanins / Q000378:metabolism*; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D027845:Malus / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins; D010088:Oxidoreductases; D010105:Oxygenases; C118159:anthocyanidin synthase; C085313:flavonol synthase; D005964:Glucosyltransferases; C023249:flavonol 3-O-glucosyltransferase