title: Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major.
abstract: Leishmaniasis is a vector-borne disease caused by protozoan parasites of the genus Leishmania. Our knowledge of protein phosphatases (PPs) and their implication in signaling events is very limited. Here we report the expression, characterization and mutagenesis analysis of a novel protein phosphatase 5 (PP5) in Leishmania major. Recombinant PP5 is a bona fide phosphatase and is enzymatically active. Site-directed mutagenesis revealed auto-inhibitory roles of the N-terminal region. This is a rational first approach to understand the role of PP5 in the biology of the parasite better as well as its potential future applicability to anti-parasitic intervention.
authors.affiliation: Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46556, USA.
authors.forename: Brianna
authors.initials: B
authors.lastname: Norris-Mullins
authors.affiliation: Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46556, USA.
authors.forename: Paola
authors.initials: P
authors.lastname: Vacchina
authors.affiliation: Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46556, USA.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Morales
mesh_terms: D020816:Amino Acid Motifs; D000814:Aniline Compounds / Q000378:metabolism; D002384:Catalysis; D004789:Enzyme Activation; D017125:Genes, Protozoan; D018320:Leishmania major / Q000201:enzymology* / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D009687:Nuclear Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D000814:Aniline Compounds; D009687:Nuclear Proteins; D009943:Organophosphorus Compounds; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; C068798:4-aminophenylphosphate; D010749:Phosphoprotein Phosphatases; C089641:protein phosphatase 5