title: Seminal plasma proteome of electroejaculated Bos indicus bulls.
abstract: The present study describes the seminal plasma proteome of Bos indicus bulls. Fifty-six, 24-month old Australian Brahman sires were evaluated and subjected to electroejaculation. Seminal plasma proteins were separated by 2-D SDS-PAGE and identified by mass spectrometry. The percentage of progressively motile and morphologically normal sperm of the bulls were 70.4 ± 2.3 and 64 ± 3.2%, respectively. A total of 108 spots were identified in the 2-D maps, corresponding to 46 proteins. Binder of sperm proteins accounted for 55.8% of all spots detected in the maps and spermadhesins comprised the second most abundant constituents. Other proteins of the Bos indicus seminal plasma include clusterin, albumin, transferrin, metalloproteinase inhibitor 2, osteopontin, epididymal secretory protein E1, apolipoprotein A-1, heat shock 70 kDa protein, glutathione peroxidase 3, cathelicidins, alpha-enolase, tripeptidyl-peptidase 1, zinc-alpha-2-glycoprotein, plasma serine protease inhibitor, beta 2-microglobulin, proteasome subunit beta type-4, actin, cathepsins, nucleobinding-1, protein S100-A9, hemoglobin subunit alpha, cadherin-1, angiogenin-1, fibrinogen alpha and beta chain, ephirin-A1, protein DJ-1, serpin A3-7, alpha-2-macroglobulin, annexin A1, complement factor B, polymeric immunoglobulin receptor, seminal ribonuclease, ribonuclease-4, prostaglandin-H2 d-isomerase, platelet-activating factor acetylhydrolase, and phosphoglycerate kinase 1. In conclusion, this work uniquely portrays the Bos indicus seminal fluid proteome, based on samples from a large set of animals representing the Brahman cattle of the tropical Northern Australia. Based on putative biochemical attributes, seminal proteins act during sperm maturation, protection, capacitation and fertilization.
authors.affiliation: Department of Animal Science, The Federal University of Ceará, Brazil. Electronic address: joaopaulo.rego@gmail.com.
authors.forename: J P A
authors.initials: JP
authors.lastname: Rego
authors.affiliation: School of Veterinary Science, The University of Queensland, Queensland 4343, Australia.
authors.forename: J M
authors.initials: JM
authors.lastname: Crisp
authors.affiliation: Department of Animal Science, The Federal University of Ceará, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Moura
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, Queensland 4072, Australia.
authors.forename: A S
authors.initials: AS
authors.lastname: Nouwens
authors.affiliation: CSIRO Animal, Food and Health Sciences, Queensland 4067, Australia.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Agri-Science Department of Agriculture, Fisheries and Forestry, Queensland 4000, Australia.
authors.forename: B
authors.initials: B
authors.lastname: Venus
authors.affiliation: Agri-Science Department of Agriculture, Fisheries and Forestry, Queensland 4701, Australia.
authors.forename: N J
authors.initials: NJ
authors.lastname: Corbet
authors.affiliation: Agri-Science Department of Agriculture, Fisheries and Forestry, Queensland 4701, Australia.
authors.forename: D H
authors.initials: DH
authors.lastname: Corbet
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, Queensland 4701, Australia.
authors.forename: B M
authors.initials: BM
authors.lastname: Burns
authors.affiliation: School of Veterinary Science, The University of Queensland, Queensland 4343, Australia.
authors.forename: G B
authors.initials: GB
authors.lastname: Boe-Hansen
authors.affiliation: School of Veterinary Science, The University of Queensland, Queensland 4343, Australia.
authors.forename: M R
authors.initials: MR
authors.lastname: McGowan
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D004542:Ejaculation / Q000502:physiology*; D004558:Electric Stimulation; D008297:Male; D020543:Proteome / Q000737:chemistry*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000737:chemistry*
chemical_list: D020543:Proteome; D029589:Seminal Plasma Proteins
keywords: Bos indicus; Electrophoresis; Proteome; Seminal plasma; Sperm