title: Metadherin regulates proliferation and metastasis via actin cytoskeletal remodelling in non-small cell lung cancer.
abstract: BACKGROUND
  Metaderin (MTDH) protein is a novel component part of tight junction complex. The aim of this study was to investigate the correlation between MTDH and prognosis of patients and to explore the role of MTDH on NSCLC development and metastasis.
  
  
  METHODS
  Relative mRNA expression was evaluated by quantitative real-time PCR, and protein expression was detected using immunohistochemistry staining. The role of MTDH in cancer cell proliferation, migration and invasion was studied by modulation of MTDH expression in NSCLC cell lines. These functions of MTDH were further confirmed in vivo.
  
  
  RESULTS
  In NSCLC, low MTDH protein expression was correlated with lymph node metastasis, TNM stage and decreased OS (P=0.001, 0.011 and 0.013, respectively). Overexpression of MTDH reduced anchorage-independent and -dependent growth through arresting cell cycle, inhibited migration and invasion in vitro and further suppressed tumorigenesis, tumour growth and metastasis in vivo. Knockdown of MTDH expression increased cell invasiveness. MTDH overexpression reversed pro-metastatic actin cytoskeleton remodelling and inhibited EMT, supporting that MTDH has a key role on cancer proliferation and metastasis.
  
  
  CONCLUSIONS
  MTDH has an important role in NSCLC proliferation and metastasis and provides potential in predicting metastasis and prognosis for patients with NSCLC.
authors.affiliation: Jinling Hospital, Department of Respiratory Medicine, Nanjing University, School of Medicine, Nanjing 210002, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Jinling Hospital, Department of Respiratory Medicine, Nanjing University, School of Medicine, Nanjing 210002, China.
authors.forename: X
authors.initials: X
authors.lastname: Gu
authors.affiliation: Jinling Hospital, Department of Respiratory Medicine, Nanjing University, School of Medicine, Nanjing 210002, China.
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.affiliation: Jinling Hospital, Department of Respiratory Medicine, Nanjing University, School of Medicine, Nanjing 210002, China.
authors.forename: G
authors.initials: G
authors.lastname: Wu
authors.affiliation: Jinling Hospital, Department of Respiratory Medicine, Nanjing University, School of Medicine, Nanjing 210002, China.
authors.forename: D
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Division of Medical Oncology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.affiliation: Jinling Hospital, Department of Respiratory Medicine, Nanjing University, School of Medicine, Nanjing 210002, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Song
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011379:Prognosis
chemical_list: D000199:Actins