title: A new, bioactive, antibacterial-eluting, composite graft for infection-free wound healing.
abstract: The current work focuses on the in vivo performance of a newly developed injectable composite graft in infected full-thickness wounds. The composite graft was composed of bioactive porous Poly dl-lactide-co-glycolide scaffolds, antibiotic gentamicin, and crosslinked gelatin as carrier gel. Treated infected wounds exhibited a faster wound closure, rapid weight gain, lower neutrophil count, higher breaking strength, and 100 times lesser microbial count (10(2) colony forming units/g in infected treated vs. 10(4)  colony forming units/g in infected control group) in comparison with infected control group 28 days post treatment. During healing, collagen production was more in the treated groups at day 7 than controls and thereafter gradually reduced to normal levels. Histology revealed a mature scar tissue formation, fibroblast proliferation, epidermal resurfacing, and collagen deposition in reticular alignment similar to normal healthy skin in treated wounds. Further, the plasma concentration of gentamicin was 35-45 μg/mL during the initial 12 hours and reduced to 1 μg/mL in 24 hours, which indicated safe levels of the antibiotic drug during healing. These results clearly indicate a faster, infection-free, and safe after treatment with the developed graft.
authors.affiliation: Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, India.
authors.forename: Anupama
authors.initials: A
authors.lastname: Mittal
authors.forename: Neeraj
authors.initials: N
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D040761:Biomimetic Materials; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D005780:Gelatin; D005782:Gels; D005839:Gentamicins / Q000494:pharmacology*; D011552:Pseudomonas Infections / Q000188:drug therapy*; D051381:Rats; D012867:Skin / Q000503:physiopathology; D013203:Staphylococcal Infections / Q000188:drug therapy*; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000188:drug therapy* / Q000382:microbiology / Q000503:physiopathology / Q000517:prevention & control; D014947:Wounds and Injuries / Q000382:microbiology / Q000503:physiopathology*
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D005782:Gels; D005839:Gentamicins; D005780:Gelatin