title: Ultrafast and wide range analysis of DNA molecules using rigid network structure of solid nanowires.
abstract: Analyzing sizes of DNA via electrophoresis using a gel has played an important role in the recent, rapid progress of biology and biotechnology. Although analyzing DNA over a wide range of sizes in a short time is desired, no existing electrophoresis methods have been able to fully satisfy these two requirements. Here we propose a novel method using a rigid 3D network structure composed of solid nanowires within a microchannel. This rigid network structure enables analysis of DNA under applied DC electric fields for a large DNA size range (100 bp-166 kbp) within 13 s, which are much wider and faster conditions than those of any existing methods. The network density is readily varied for the targeted DNA size range by tailoring the number of cycles of the nanowire growth only at the desired spatial position within the microchannel. The rigid dense 3D network structure with spatial density control plays an important role in determining the capability for analyzing DNA. Since the present method allows the spatial location and density of the nanostructure within the microchannels to be defined, this unique controllability offers a new strategy to develop an analytical method not only for DNA but also for other biological molecules.
authors.affiliation: 1] Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka-cho, Ibaraki, Osaka 567-0047, Japan [2].
authors.forename: Sakon
authors.initials: S
authors.lastname: Rahong
authors.affiliation: 1] Department of Applied Chemistry, Graduate School of Engineering, Nagoya University [2] FIRST Research Center for Innovative Nanobiodevices, Nagoya University [3] Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Yasui
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka-cho, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yanagida
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka-cho, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nagashima
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka-cho, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Kanai
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka-cho, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Annop
authors.initials: A
authors.lastname: Klamchuen
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka-cho, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Gang
authors.initials: G
authors.lastname: Meng
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka-cho, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Yong
authors.initials: Y
authors.lastname: He
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka-cho, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Fuwei
authors.initials: F
authors.lastname: Zhuge
authors.affiliation: 1] Department of Applied Chemistry, Graduate School of Engineering, Nagoya University [2] FIRST Research Center for Innovative Nanobiodevices, Nagoya University [3] Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka-cho, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Tomoji
authors.initials: T
authors.lastname: Kawai
authors.affiliation: 1] Department of Applied Chemistry, Graduate School of Engineering, Nagoya University [2] FIRST Research Center for Innovative Nanobiodevices, Nagoya University [3] Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan [4] Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry*
chemical_list: D004247:DNA