title: Application of an electrochemical immunosensor with a MWCNT/PDAA modified electrode for detection of serum trypsin.
abstract: OBJECTIVE
  To establish an electrochemical immunosensor for the determination of serum trypsin levels using a multiwall carbon nanotubes (MWCNTs)-composite-modified electrode.
  
  
  METHODS
  A MWCNT composite coated on the surface of bare gold electrodes was used for fixation of an anti-trypsin antibody. The assembly process and the performance indicators, including sensitivity, linear range of detection, anti-jamming performance, and stability, of the electrochemical immunosensor were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).
  
  
  RESULTS
  With optimized experimental conditions, the difference of the current value measured by differential pulse voltammetry (DPV) showed a linear relationship with the concentration of serum trypsin within 0.10-100 ng/mL. The detection limit for trypsin using this sensor was 0.002 ng/mL.
  
  
  CONCLUSIONS
  The electrochemical immunosensor built using the MWCNT-composite-modified electrode is simple to operate and has a fast response time, along with a wide linear range, high sensitivity, and accuracy, making it suitable for serum trypsin detection.
authors.affiliation: Department of Laboratory Medicine, the 1st Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China. yiqiang8798@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yi
authors.affiliation: Department of Laboratory Medicine, the 1st Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China. lqc673673673@163.com.
authors.forename: Qicai
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Pathology, the 1st Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China. fengfang77777@163.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of Laboratory Medicine, College of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350005, China. cqq036@fjmu.edu.cn.
authors.forename: Qingquan
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, College of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350005, China. wangguina8798@163.com.
authors.forename: Guina
authors.initials: G
authors.lastname: Wang
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D006046:Gold; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; D014357:Trypsin / Q000097:blood*
chemical_list: D037742:Nanotubes, Carbon; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D006046:Gold; D014357:Trypsin