title: Description of Domibacillus indicus sp. nov., isolated from ocean sediments and emended description of the genus Domibacillus.
abstract: A novel Gram-stain-positive, spore-forming, aerobic, non-motile, rod-shaped bacterium designated strain SD111(T) that forms red-pigmented colonies was isolated from a marine sediment sample (collected from 5 m depth) from Lakshadweep, India. Strain SD111(T) grew well on seawater agar at pH 6-10 (optimum pH 7.5±0.2). It showed maximum (97.6 %) 16S rRNA gene sequence similarity and formed a monophyletic clade with Domibacillus robiginosus WS 4628(T) ( = DSM 25058(T)). The genomic DNA G+C content was 37.4 mol% and the strain showed 37.7 % DNA-DNA relatedness to D. robiginosus DSM 25058(T). The major fatty acids were anteiso-C15 : 0, C16 : 0, iso-C15 : 0 and iso-C16 : 0 and MK-6 was the predominant quinone. The polar lipid profile of strain SD111(T) consisted of unidentified phospholipids (PL1 and PL2), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG). The cell wall contained meso-diaminopimelic acid and the peptidoglycan was of A1γ type. Glucose and ribose were detected as major cell-wall sugars. Results from polyphasic studies indicated that SD111(T) represents a novel species of the genus Domibacillus for which the name Domibacillus indicus sp. nov. is proposed. The type strain is SD111(T) ( = MCC 2255(T) = DSM 28032(T)).
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Avinash
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Dhar
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Om
authors.initials: O
authors.lastname: Prakash
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Venkata Ramana
authors.initials: VR
authors.lastname: Vemuluri
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Vishal
authors.initials: V
authors.lastname: Thite
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D007194:India; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C042455:menaquinone 6