title: Daily variations in the glycerol-3-phosphate dehydrogenase isoforms expression in Triatoma infestans flight muscles.
abstract: Triatoma infestans, the main vector of Chagas disease, is a blood-sucking insect. Flight dispersal of adults is the most important mechanism for reinfestation of houses after insecticide spraying. Flight muscles have two glycerol-3-phosphate dehydrogenase (GPDH) isoforms: GPDH-1 is involved in flight metabolism and GPDH-2 provides lipid precursors. In this study, we explored the profile of GPDH expression in females and males adult flight muscles under light/dark cycle, constant light, and constant dark conditions. Under constant dark conditions, GPDH-1 flight muscles of T. infestans showed a rhythmic pattern of transcription synchronous with a rhythmic profile of activity suggesting regulation by the endogenous circadian clock. Otherwise, the GPDH-2 expression analysis showed no regulation by the endogenous clock, but showed that an external factor, such as the dark/light period, was necessary for synchronization of GPDH-2 transcription and activity.
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud, Consejo Nacional de Investigaciones Científicas y Técnicas and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina mercedesstroppa@hotmail.com.
authors.forename: María M
authors.initials: MM
authors.lastname: Stroppa
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud, Consejo Nacional de Investigaciones Científicas y Técnicas and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Carlota S
authors.initials: CS
authors.lastname: Carriazo
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud, Consejo Nacional de Investigaciones Científicas y Técnicas and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Nelia M
authors.initials: NM
authors.lastname: Gerez de Burgos
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud, Consejo Nacional de Investigaciones Científicas y Técnicas and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Beatríz A
authors.initials: BA
authors.lastname: Garcia
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000635:transmission; D057906:Circadian Clocks / Q000235:genetics*; D005260:Female; D005426:Flight, Animal / Q000502:physiology; D005786:Gene Expression Regulation; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007303:Insect Vectors; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008027:Light; D008297:Male; D009132:Muscles / Q000201:enzymology; D017440:Photoperiod; D014158:Transcription, Genetic; D014224:Triatoma / Q000201:enzymology / Q000235:genetics*
chemical_list: D019476:Insect Proteins; D007527:Isoenzymes; D005993:Glycerolphosphate Dehydrogenase