title: Organotypic culture to assess cell adhesion, growth and alignment of different organs on silk fibroin.
abstract: Glass sheets covered with aligned electrospun silk fibroin (Bombyx mori) were compared to tissue culture-treated Thermanox® coverslips, using an organotypic culture method. Different chick embryo organ behaviours were analysed in terms of circularity, cell growth and cell adhesion after being cultivated in contact with these two materials. The circularity (cell layer shape corresponding to the trend of the biomaterials to induce a specific directionality) depends on the organ used when in contact with silk fibroin. This biomaterial induced higher cell adhesion (kidney) or lower cell adhesion (spine) compared to Thermanox. Cell growth, represented by the cell layer area (mm2), was also drastically reduced (gonad) or increased (blood vessel) on the silk fibroin. Organotypic culture is a rapid, cost effective and relatively simple method to evaluate different parameters, allowing prescreening of morphology and cytocompatibility to select the appropriate applications for new biomaterials. In the present study we compared the morphology of different organotypic cultures on orientated silk and Thermanox as growth supports to rapidly evaluate the benefit of a silk-based biomaterial for tissue engineering. Copyright © 2014 John Wiley & Sons, Ltd.
authors.affiliation: Laboratoire BioMécanique et BioIngénierie (BMBI), UMR CNRS 7338, Université de Technologie de Compiègne, Compiègne, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Duval
authors.affiliation: Laboratoire BioMécanique et BioIngénierie (BMBI), UMR CNRS 7338, Université de Technologie de Compiègne, Compiègne, France.|Biomedical Engineering, Science and Technology Center, Tufts University, Medford, MA, USA.
authors.forename: Tony
authors.initials: T
authors.lastname: Dinis
authors.affiliation: Laboratoire BioMécanique et BioIngénierie (BMBI), UMR CNRS 7338, Université de Technologie de Compiègne, Compiègne, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Vidal
authors.affiliation: Laboratoire BioMécanique et BioIngénierie (BMBI), UMR CNRS 7338, Université de Technologie de Compiègne, Compiègne, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Vigneron
authors.affiliation: Biomedical Engineering, Science and Technology Center, Tufts University, Medford, MA, USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.affiliation: Laboratoire BioMécanique et BioIngénierie (BMBI), UMR CNRS 7338, Université de Technologie de Compiègne, Compiègne, France.|Department of Oral and Maxillofacial Pathology, Tufts University School of Dental Medicine, Boston, MA, USA.
authors.forename: Christophe
authors.initials: C
authors.lastname: Egles
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D012831:Bombyx; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D002642:Chick Embryo; D005347:Fibroblasts / Q000166:cytology; D005349:Fibroins / Q000737:chemistry*; D009924:Organ Culture Techniques; D011093:Polyethylene Terephthalates / Q000737:chemistry; D013696:Temperature; D014018:Tissue Distribution; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011093:Polyethylene Terephthalates; C500874:Thermanox; D005349:Fibroins
keywords: biocompatibility; cell adhesion; cell morphology; electrospinning; organotypic culture; silk fibroin