title: MMP-14 is necessary but not sufficient for invasion of three-dimensional collagen by human muscle satellite cells.
abstract: The twenty-five known matrix metalloproteases (MMPs) and their endogenous inhibitors, tissue inhibitors of metalloproteases (TIMPs), mediate cell invasion through the extracellular matrix (ECM). In a comparative three-dimensional assay, we analyzed human and mouse satellite cells' competence to invade an artificial ECM (collagen I). We identified a single MMP that 1) is expressed by human muscle satellite cells; 2) is induced at the mRNA/protein level by adhesion to collagen I; and 3) is necessary for invasion into a collagen I matrix. Interestingly, murine satellite cells neither express this MMP, nor invade the collagen matrix. However, exogenous human MMP-14 is not sufficient to induce invasion of a collagen matrix by murine cells, emphasizing species differences.
authors.affiliation: Division of Biology and Bond Life Sciences Center, University of Missouri, Columbia, Missouri; and.
authors.forename: Dane K
authors.initials: DK
authors.lastname: Lund
authors.affiliation: Institut de Myologie, Université Pierre et Marie Curie, Paris, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Mouly
authors.affiliation: Division of Biology and Bond Life Sciences Center, University of Missouri, Columbia, Missouri; and cornelisond@missouri.edu.
authors.forename: D D W
authors.initials: DD
authors.lastname: Cornelison
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D003094:Collagen / Q000737:chemistry*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology*; D013045:Species Specificity; D054457:Tissue Scaffolds
chemical_list: D003094:Collagen; D053511:Matrix Metalloproteinase 14
keywords: MMP-14; cellular invasion; collagen remodeling; extracellular matrix; muscle regeneration; satellite cells; skeletal muscle