title: High-density 3D single molecular analysis based on compressed sensing.
abstract: Single molecule fitting-based superresolution microscopy achieves sub-diffraction-limit image resolution but suffers from a need for long acquisition times to gather enough molecules. Several methods have recently been developed that analyze high molecule density images but most are only applicable to two dimensions. In this study, we implemented a high-density superresolution localization algorithm based on compressed sensing and a biplane approach that provides three-dimensional information about molecules, achieving super-resolution imaging at higher molecule densities than those achieved using the conventional single molecule fitting method.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, P. R. China; National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Lusheng
authors.initials: L
authors.lastname: Gu
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, P. R. China; National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sheng
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P. R. China; School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Yongdeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Lv
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P. R. China. Electronic address: jiwei@moon.ibp.ac.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, P. R. China; National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P. R. China. Electronic address: xutao@ibp.ac.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
mesh_terms: D000465:Algorithms; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D007089:Image Enhancement / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods