title: Parvovirus B19 VLP recognizes globoside in supported lipid bilayers.
abstract: Studies have suggested that the glycosphingolipid globoside (Gb4Cer) is a receptor for human parvovirus B19. Virus-like particles bind to Gb4Cer on thin-layer chromatograms, but a direct interaction between the virus and lipid membrane-associated Gb4Cer has been debated. Here, we characterized the binding of parvovirus B19 VP1/VP2 virus-like particles to glycosphingolipids (i) on thin-layer chromatograms (TLCs) and (ii) incorporated into supported lipid bilayers (SLBs) acting as cell-membrane mimics. The binding specificities of parvovirus B19 determined in the two systems were in good agreement; the VLP recognized both Gb4Cer and the Forssman glycosphingolipid on TLCs and in SLBs compatible with the role of Gb4Cer as a receptor for this virus.
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg, Bruna Stråket 16, 413 45 Gothenburg, Sweden.
authors.forename: Waqas
authors.initials: W
authors.lastname: Nasir
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg, Bruna Stråket 16, 413 45 Gothenburg, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Nilsson
authors.affiliation: Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Guldhedsg. 10B, 413 46 Gothenburg, Sweden.
authors.forename: Sigvard
authors.initials: S
authors.lastname: Olofsson
authors.affiliation: Department of Applied Physics, Chalmers University of Technology, 411 33 Gothenburg, Sweden; Institut Curie, Centre de Recherche, CNRS, UMR 168, Physico-Chimie Curie, F-75248 Paris, France.
authors.forename: Marta
authors.initials: M
authors.lastname: Bally
authors.affiliation: Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Guldhedsg. 10B, 413 46 Gothenburg, Sweden. Electronic address: gustaf.rydell@gu.se.
authors.forename: Gustaf E
authors.initials: GE
authors.lastname: Rydell
mesh_terms: D036022:Capsid Proteins / Q000378:metabolism; D005915:Globosides / Q000378:metabolism*; D008051:Lipid Bilayers / Q000378:metabolism*; D016732:Parvovirus B19, Human / Q000502:physiology*; D011991:Receptors, Virus / Q000378:metabolism*; D022701:Virosomes / Q000378:metabolism; D053585:Virus Attachment
chemical_list: D036022:Capsid Proteins; D005915:Globosides; D008051:Lipid Bilayers; D011991:Receptors, Virus; D022701:Virosomes; C095547:capsid protein VP1, parvovirus B19; C095548:capsid protein VP2, parvovirus B19
keywords: Carbohydrate; Glycan; Glycobiology; Glycosphingolipids; Parvovirus B19 virus-like particles