title: Large-scale gene expression profiling data for the model moss Physcomitrella patens aid understanding of developmental progression, culture and stress conditions.
abstract: The moss Physcomitrella patens is an important model organism for studying plant evolution, development, physiology and biotechnology. Here we have generated microarray gene expression data covering the principal developmental stages, culture forms and some environmental/stress conditions. Example analyses of developmental stages and growth conditions as well as abiotic stress treatments demonstrate that (i) growth stage is dominant over culture conditions, (ii) liquid culture is not stressful for the plant, (iii) low pH might aid protoplastation by reduced expression of cell wall structure genes, (iv) largely the same gene pool mediates response to dehydration and rehydration, and (v) AP2/EREBP transcription factors play important roles in stress response reactions. With regard to the AP2 gene family, phylogenetic analysis and comparison with Arabidopsis thaliana shows commonalities as well as uniquely expressed family members under drought, light perturbations and protoplastation. Gene expression profiles for P. patens are available for the scientific community via the easy-to-use tool at https://www.genevestigator.com. By providing large-scale expression profiles, the usability of this model organism is further enhanced, for example by enabling selection of control genes for quantitative real-time PCR. Now, gene expression levels across a broad range of conditions can be accessed online for P. patens.
authors.affiliation: Plant Cell Biology, Faculty of Biology, University of Marburg, Karl-von-Frisch-Strasse 8, 35043, Marburg, Germany; Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104, Freiburg, Germany; FRISYS Freiburg Initiative for Systems Biology, University of Freiburg, 79104, Freiburg, Germany.
authors.forename: Manuel
authors.initials: M
authors.lastname: Hiss
authors.forename: Oliver
authors.initials: O
authors.lastname: Laule
authors.forename: Rasa M
authors.initials: RM
authors.lastname: Meskauskiene
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Arif
authors.forename: Eva L
authors.initials: EL
authors.lastname: Decker
authors.forename: Anika
authors.initials: A
authors.lastname: Erxleben
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Frank
authors.forename: Sebastian T
authors.initials: ST
authors.lastname: Hanke
authors.forename: Daniel
authors.initials: D
authors.lastname: Lang
authors.forename: Anja
authors.initials: A
authors.lastname: Martin
authors.forename: Christina
authors.initials: C
authors.lastname: Neu
authors.forename: Ralf
authors.initials: R
authors.lastname: Reski
authors.forename: Sandra
authors.initials: S
authors.lastname: Richardt
authors.forename: Mareike
authors.initials: M
authors.lastname: Schallenberg-Rüdinger
authors.forename: Peter
authors.initials: P
authors.lastname: Szövényi
authors.forename: Theodhor
authors.initials: T
authors.lastname: Tiko
authors.forename: Gertrud
authors.initials: G
authors.lastname: Wiedemann
authors.forename: Luise
authors.initials: L
authors.lastname: Wolf
authors.forename: Philip
authors.initials: P
authors.lastname: Zimmermann
authors.forename: Stefan A
authors.initials: SA
authors.lastname: Rensing
mesh_terms: D019068:Bryopsida / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*
keywords: Physcomitrella patens; culture; development; gene expression; genevestigator; microarray; moss; stress; transcriptomics