title: Oral exposure of pubertal male mice to endocrine-disrupting chemicals alters fat metabolism in adult livers.
abstract: The potential for the exposure of humans and wildlife to environmental endocrine-disrupting chemicals (EDCs) has been increasing. Risk assessment for such EDCs is primarily based on detecting the main endpoints related to the endocrine and reproductive systems, while the effects on glucose and fat metabolism have only received limited attention. In this study, pubertal male C57BL/6J mice were orally administered 10 mg/kg body weight cypermethrin (CYP), 100 mg/kg body weight atrazine (ATZ), and 0.1 mg/kg body weight 17α-ethynyestradiol (EE2) for 4 weeks and then switched to a high-energy diet (HD) for 8 weeks. The body weight gain in the EDC-treated groups was lower than that in the control group during exposure and then tended to show values similar to the HD group. The epididymal fat weight, cell size and serum triacylglycerol (TG) and total cholesterol (TCH) levels in the EDC-HD groups were lower than those in the HD group. The transcription of genes related to glycolytic and gluconeogenic processes in the liver was affected by EDC exposure. Furthermore, the expression levels of transcriptional factors including PPARα, PPARγ, and SREBP1C and their target genes related to fatty acid synthesis and oxidation in the liver were also influenced by early life EDC administration. The results showed that early-life-stage exposure to high doses of various environmental EDCs affected the homeostasis of glucose and fatty acid metabolism in the livers of adult male mice.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Yuanxiang
authors.initials: Y
authors.lastname: Jin
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Lin
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Miao
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Linggang
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Fu
mesh_terms: D006907:17-alpha-Hydroxypregnenolone / Q000633:toxicity*; D000284:Administration, Oral; D000818:Animals; D001280:Atrazine / Q000633:toxicity; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D052244:Endocrine Disruptors / Q000633:toxicity*; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011722:Pyrethrins / Q000633:toxicity; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D052244:Endocrine Disruptors; D011722:Pyrethrins; D014280:Triglycerides; C017160:cypermethrin; D006907:17-alpha-Hydroxypregnenolone; D002784:Cholesterol; D001280:Atrazine
keywords: early life exposure; endocrine disruption chemicals; fat; glucose; metabolism; mice