title: Characterization of glucose oxidation by gold nanoparticles using nanoceria.
abstract: Gold nanoparticles (AuNPs) can oxidize glucose, producing hydrogen peroxide and gluconic acid, which are the same products as those generated by glucose oxidase (GOx). In this regard, AuNPs are a nanozyme. Herein, a new colorimetric method is developed to understand the surface chemistry of gold nanoparticles for this oxidation reaction. The color of nanoceria is changed to yellow by the hydrogen peroxide generated during glucose oxidation. Using this assay, we find that adsorption of small molecules such as citrate does not deactivate AuNPs, while adsorption of polymers including serum proteins and high molecular weight polyethylene glycol inhibits glucose oxidation. In addition to glucose, AuNPs can also oxidize galactose. Therefore, this reaction is unlikely to be directly useful for glucose detection for biomedical applications. On the other hand, AuNPs might serve as a general oxidase for a broad range of substrates. The glucose oxidation reaction is slower at lower pH. Since the reaction generates an acid product, glucose oxidation becomes slower as the reaction proceeds. The effects of temperature, AuNP size, and reaction kinetics have been systematically studied. This work provides new insights regarding the surface chemistry of AuNPs as a nanozyme.
authors.affiliation: Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Lang
authors.affiliation: Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
authors.forename: Biwu
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada. Electronic address: liujw@uwaterloo.ca.
authors.forename: Juewen
authors.initials: J
authors.lastname: Liu
mesh_terms: D002563:Cerium / Q000737:chemistry*; D003124:Colorimetry; D005947:Glucose / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction
chemical_list: D002563:Cerium; C030583:ceric oxide; D006046:Gold; D006861:Hydrogen Peroxide; D005947:Glucose
keywords: Biosensors; Cerium oxide; Glucose; Gold nanoparticles; Nanozymes