title: Population structure of KPC-producing Klebsiella pneumoniae isolates from midwestern U.S. hospitals.
abstract: Genome sequencing of carbapenem-resistant Klebsiella pneumoniae isolates from regional U.S. hospitals was used to characterize strain diversity and the bla(KPC) genetic context. A phylogeny based on core single-nucleotide variants (SNVs) supports a division of sequence type 258 (ST258) into two distinct groups. The primary differences between the groups are in the capsular polysaccharide locus (cps) and their plasmid contents. A strict association between clade and KPC variant was found. The bla(KPC) gene was found on variants of two plasmid backbones. This study indicates that highly similar K. pneumoniae subpopulations coexist within the same hospitals over time.
authors.affiliation: J. Craig Venter Institute, La Jolla, California, USA.
authors.forename: Meredith S
authors.initials: MS
authors.lastname: Wright
authors.affiliation: Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
authors.forename: Federico
authors.initials: F
authors.lastname: Perez
authors.affiliation: J. Craig Venter Institute, La Jolla, California, USA.
authors.forename: Lauren
authors.initials: L
authors.lastname: Brinkac
authors.affiliation: Department of Pathology, Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, Ohio, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Jacobs
authors.affiliation: Detroit Medical Center, Detroit, Michigan, USA.
authors.forename: Keith
authors.initials: K
authors.lastname: Kaye
authors.affiliation: Infectious Diseases Department, Cleveland Clinic, Cleveland, Ohio, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Cober
authors.affiliation: Division of Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: David
authors.initials: D
authors.lastname: van Duin
authors.affiliation: Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
authors.forename: Steven H
authors.initials: SH
authors.lastname: Marshall
authors.affiliation: Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Hujer
authors.affiliation: Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: Susan D
authors.initials: SD
authors.lastname: Rudin
authors.affiliation: Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: Kristine M
authors.initials: KM
authors.lastname: Hujer
authors.affiliation: Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio, USA Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA madams@jcvi.org Robert.Bonomo@va.gov.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Bonomo
authors.affiliation: J. Craig Venter Institute, La Jolla, California, USA madams@jcvi.org Robert.Bonomo@va.gov.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Adams
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D015780:Carbapenems / Q000494:pharmacology; D006761:Hospitals; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D015146:Midwestern United States / Q000453:epidemiology; D010802:Phylogeny; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D011135:Polysaccharides, Bacterial; D001618:beta-Lactamases