title: Elucidating the host-pathogen interaction between human colorectal cells and invading Enterovirus 71 using transcriptomics profiling.
abstract: Enterovirus 71 (EV71) is one of the main etiological agents for Hand, Foot and Mouth Disease (HFMD) and has been shown to be associated with severe clinical manifestation. Currently, there is no antiviral therapeutic for the treatment of HFMD patients owing to a lack of understanding of EV71 pathogenesis. This study seeks to elucidate the transcriptomic changes that result from EV71 infection. Human whole genome microarray was employed to monitor changes in genomic profiles between infected and uninfected cells. The results reveal altered expression of human genes involved in critical pathways including the immune response and the stress response. Together, data from this study provide valuable insights into the host-pathogen interaction between human colorectal cells and EV71.
authors.affiliation: School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Queensland, Australia ; Institute of Health and Biomedical Innovation, Queensland University of Technology, Queensland, Australia ; School of Chemical and Life Sciences, Singapore Polytechnic, Singapore ; Centre for Biomedical and Life Sciences, Singapore Polytechnic, Singapore.
authors.forename: Yan Long Edmund
authors.initials: YL
authors.lastname: Lui
authors.affiliation: School of Chemical and Life Sciences, Singapore Polytechnic, Singapore.
authors.forename: Tuan Lin
authors.initials: TL
authors.lastname: Tan
authors.affiliation: School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Queensland, Australia ; Institute of Health and Biomedical Innovation, Queensland University of Technology, Queensland, Australia ; Faculty of Science, Health, Education & Engineering, University of the Sunshine Coast, Queensland, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Timms
authors.affiliation: School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Queensland, Australia ; Institute of Health and Biomedical Innovation, Queensland University of Technology, Queensland, Australia.
authors.forename: Louise Marie
authors.initials: LM
authors.lastname: Hafner
authors.affiliation: School of Chemical and Life Sciences, Singapore Polytechnic, Singapore.
authors.forename: Kian Hwa
authors.initials: KH
authors.lastname: Tan
authors.affiliation: Centre for Biomedical and Life Sciences, Singapore Polytechnic, Singapore ; Department of Paediatrics, University Children's Medical Institute, National University Hospital, Singapore.
authors.forename: Eng Lee
authors.initials: EL
authors.lastname: Tan
keywords: Colorectal cells; EV71, Enterovirus 71; Enterovirus 71; HFMD, Hand, Foot and Mouth Disease; Hand, Foot and Mouth Disease; ISG, interferon stimulated genes; MOI, multiplicity of infection; Microarray; RD, rhabdomyosarcoma; Transcriptomics; mRNA profiling