title: Protective effects of lipoxin A4 in testis injury following testicular torsion and detorsion in rats.
abstract: OBJECTIVE
  To investigate the protective effects of lipoxin A4 (LXA4) in rat testis injury following testicular torsion/detorsion.
  
  
  METHODS
  A rat testicular torsion model has been established as described. Rats were randomly divided into 6 groups: sham group, torsion group, torsion/detorsion (T/D) group, and T/D plus LXA4-pretreated groups (3 subgroups). Rats in LXA4-pretreated groups received LXA4 injection (0.1, 1.0, and 10 μg/kg body weight in LXA4-pretreated subgroups 1-3, resp.) at a single dose 1 h before detorsion. Biochemical analysis, apoptosis assessment, and morphologic evaluation were carried out after orchiectomies.
  
  
  RESULTS
  GPx and SOD levels significantly increased and MDA levels significantly reduced in LXA4-pretreated groups compared to T/D group. LXA4 also reverted IL-2 and TNF- α to basal levels and improved the expression of IL-4 and IL-10 in LXA4-pretreated groups. Moreover, the expression of NF- κ B was downregulated in LXA4-pretreated groups. LXA4 treatment also showed an improved testicular morphology and decreased apoptosis in testes.
  
  
  CONCLUSIONS
  Lipoxin A4 protects rats against testes injury after torsion/detorsion via modulation of cytokines, oxidative stress, and NF- κ B activity.
authors.affiliation: Emergency Center, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuchang, Wuhan, Hubei 430071, China.
authors.forename: Xian-Long
authors.initials: XL
authors.lastname: Zhou
authors.affiliation: Emergency Center, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuchang, Wuhan, Hubei 430071, China.
authors.forename: Qi-Sheng
authors.initials: QS
authors.lastname: Yang
authors.affiliation: Emergency Center, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuchang, Wuhan, Hubei 430071, China.
authors.forename: Shao-Zhou
authors.initials: SZ
authors.lastname: Ni
authors.affiliation: Emergency Center, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuchang, Wuhan, Hubei 430071, China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Tu
authors.affiliation: Emergency Center, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuchang, Wuhan, Hubei 430071, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Emergency Center, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuchang, Wuhan, Hubei 430071, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Emergency Center, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuchang, Wuhan, Hubei 430071, China.
authors.forename: Zheng-Qi
authors.initials: ZQ
authors.lastname: Pan
authors.affiliation: Emergency Center, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuchang, Wuhan, Hubei 430071, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D017209:Apoptosis; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation; D005979:Glutathione Peroxidase / Q000378:metabolism; D016753:Interleukin-10 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D044045:Lipoxins / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000737:chemistry; D016328:NF-kappa B / Q000097:blood; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D013086:Spermatic Cord Torsion / Q000473:pathology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D044045:Lipoxins; D016328:NF-kappa B; C040527:lipoxin A4; D016753:Interleukin-10; D015847:Interleukin-4; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase