title: Aerobic transformation of BDE-47 by a Pseudomonas putida sp. strain TZ-1 isolated from PBDEs-contaminated sediment.
abstract: A bacterial isolate, TZ-1, was isolated from contaminated sediment near electronic waste dismantling workshops, Taizhou, China that degraded 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47). The isolate was identified as Pseudomonas putida sp. with respect to its morphology, biochemical characteristics and 16SrDNA sequence analysis. TZ-1 can use BDE-47 as the sole carbon and energy source for growth in mineral salt medium. The isolate degraded BDE-47 up to 49.96 % of the initially applied concentration of 50 μg L(-1) after 7 days of incubation at 150 rpm, 30°C. Static conditions with pH 6.5 and temperature 30°C were considered to be optimum for BDE-47 biodegradation. Addition of co-substrates promoted cell growth, but decreased the degradation rate for BDE-47.
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China, xinjia58@163.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Xin
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xi-Lai
authors.initials: XL
authors.lastname: Zheng
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D001711:Biotransformation; D002681:China; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D059029:Electronic Waste / Q000032:analysis; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D055768:Halogenated Diphenyl Ethers / Q000032:analysis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D013696:Temperature
chemical_list: D017931:DNA Primers; D055768:Halogenated Diphenyl Ethers; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C511295:2,2',4,4'-tetrabromodiphenyl ether