title: Green tea polyphenol, epigallocatechin-3-gallate, possesses the antiviral activity necessary to fight against the hepatitis B virus replication in vitro.
abstract: Although several antiviral drugs and vaccines are available for use against hepatitis B virus (HBV), hepatitis caused by HBV remains a major public health problem worldwide, which has not yet been resolved, and new anti-HBV drugs are in great demand. The present study was performed to investigate the anti-HBV activity of epigallocatechin-3-gallate (EGCG), a natural-origin compound, in HepG2 2.2.15 cells. The antiviral activity of EGCG was examined by detecting the levels of HBsAg and HBeAg in the supernatant and extracellular HBV DNA. EGCG effectively suppressed the secretion of HBsAg and HBeAg from HepG2 2.2.15 cells in a dose- and time-dependent manner, and it showed stronger effects at the level of 0.11-0.44 μmol/ml (50-200 μg/ml) than lamivudine (3TC) at 0.87 μmol/ml (200 μg/ml). EGCG also suppressed the amount of extracellular HBV DNA. The data indicated that EGCG possessed anti-HBV activity and suggested the potential of EGCG as an effective anti-HBV agent with low toxicity.
authors.affiliation: Clinical Pharmacy Laboratory, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing 100039, China; Integrative Medicine Center, 302 Military Hospital, Beijing 100039, China.
authors.forename: Jing-yao
authors.initials: JY
authors.lastname: Pang
authors.forename: Kui-jun
authors.initials: KJ
authors.lastname: Zhao
authors.forename: Jia-bo
authors.initials: JB
authors.lastname: Wang
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Ma
authors.forename: Xiao-he
authors.initials: XH
authors.lastname: Xiao
mesh_terms: D000998:Antiviral Agents / Q000008:administration & dosage; D028241:Camellia sinensis / Q000737:chemistry; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D010936:Plant Extracts / Q000008:administration & dosage; D014779:Virus Replication / Q000187:drug effects* / Q000502:physiology*
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Antiviral activity; Epigallocatechin-3-gallate; HepG2 2.2.15; Hepatitis B virus (HBV); Lamivudine