title: An orthogonal ribosome-tRNA pair via engineering of the peptidyl transferase center.
abstract: The Watson-Crick base pairs between the 3'-terminal end of tRNAs and ribosomal RNA in the peptidyl transferase center are universally conserved. Here, we report that the introduction of compensatory mutations to Escherichia coli RNAs in this site leads to an orthogonal system independent of the wild-type counterpart, as demonstrated via the production of two peptide sequences from a single mRNA. This work thus identifies a new way to reprogram the genetic code.
authors.affiliation: 1] Department of Chemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan. [2].
authors.forename: Naohiro
authors.initials: N
authors.lastname: Terasaka
authors.affiliation: 1] Department of Chemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan. [2] [3].
authors.forename: Gosuke
authors.initials: G
authors.lastname: Hayashi
authors.affiliation: Department of Chemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Katoh
authors.affiliation: 1] Department of Chemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan. [2] Japan Science and Technology Agency, Core Research for Evolutional Science and Technology, University of Tokyo, Tokyo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suga
mesh_terms: D020029:Base Pairing; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005815:Genetic Code; D009154:Mutation; D010458:Peptidyl Transferases / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D015202:Protein Engineering; D012338:RNA, Ribosomal, 23S / Q000235:genetics* / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D012338:RNA, Ribosomal, 23S; D012343:RNA, Transfer; D010458:Peptidyl Transferases