title: Preeclampsia: integrated network model of platelet biomarkers interaction as a tool to evaluate the hemostatic/immunological interface.
abstract: BACKGROUND
  Preeclampsia (PE) is associated with platelet activation, which may be involved in its pathogenesis promoting coagulation and mediating inflammation. We investigated whether the platelet activation status together with the frequency of platelet-leukocyte aggregates/PLA and monocyte tissue factor/TF expression could be used as laboratorial biomarkers for PE diagnosis and prognosis.
  
  
  METHODS
  Ninety-seven women were evaluated including severe PE/sPE (N=15), mild PE/mPE (N=20), normotensive pregnant/NP (N=31) and non-pregnant women/nonP (N=31). Platelet markers were analyzed by flow cytometry.
  
  
  RESULTS
  Platelet counts and CD41a expression by platelets were lower in NP and sPE vs nonP. The expression of CD61 was lower during pregnancy. Altered balance of platelet marker expression was also observed in NP and sPE vs nonP. No significant differences in the PLA and TF expression by monocytes were observed among the groups. There are several correlations between platelet activation markers, especially in sPE, which suggest a relevant role of the hemostatic/immunological cross-talk in this disease.
  
  
  CONCLUSIONS
  PE is not associated with increased platelet activation markers. It cannot rule out a role of platelet activation in the PE pathophysiology. Despite those correlations, we did not find a putative laboratorial biomarker that could be useful by itself for PE diagnosis and prognosis.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia-Universidade Federal de Minas Gerais, Brazil.
authors.forename: Letícia Gonçalves
authors.initials: LG
authors.lastname: Freitas
authors.affiliation: Laboratório de Biomarcadores de Diagnóstico e Monitoração, Instituto René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Sathler-Avelar
authors.affiliation: Laboratório de Biomarcadores de Diagnóstico e Monitoração, Instituto René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Danielle Marquete
authors.initials: DM
authors.lastname: Vitelli-Avelar
authors.affiliation: Laboratório de Biomarcadores de Diagnóstico e Monitoração, Instituto René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Samantha Ribeiro
authors.initials: SR
authors.lastname: Bela
authors.affiliation: Laboratório de Biomarcadores de Diagnóstico e Monitoração, Instituto René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Andréa
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia-Universidade Federal de Minas Gerais, Brazil.
authors.forename: Maria das Graças
authors.initials: Md
authors.lastname: Carvalho
authors.affiliation: Laboratório de Biomarcadores de Diagnóstico e Monitoração, Instituto René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Olindo Assis
authors.initials: OA
authors.lastname: Martins-Filho
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia-Universidade Federal de Minas Gerais, Brazil. Electronic address: lucidusse@gmail.com.
authors.forename: Luci Maria SantAna
authors.initials: LM
authors.lastname: Dusse
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D006487:Hemostasis; D006801:Humans; D008954:Models, Biological; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D015539:Platelet Activation; D010974:Platelet Aggregation; D011225:Pre-Eclampsia / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology*; D011247:Pregnancy; D011379:Prognosis; D013925:Thromboplastin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D013925:Thromboplastin
keywords: Leukocytes; Membrane glycoproteins; P-selectin; Platelet activation; Preeclampsia