title: CELLO2GO: a web server for protein subCELlular LOcalization prediction with functional gene ontology annotation.
abstract: CELLO2GO (http://cello.life.nctu.edu.tw/cello2go/) is a publicly available, web-based system for screening various properties of a targeted protein and its subcellular localization. Herein, we describe how this platform is used to obtain a brief or detailed gene ontology (GO)-type categories, including subcellular localization(s), for the queried proteins by combining the CELLO localization-predicting and BLAST homology-searching approaches. Given a query protein sequence, CELLO2GO uses BLAST to search for homologous sequences that are GO annotated in an in-house database derived from the UniProt KnowledgeBase database. At the same time, CELLO attempts predict at least one subcellular localization on the basis of the species in which the protein is found. When homologs for the query sequence have been identified, the number of terms found for each of their GO categories, i.e., cellular compartment, molecular function, and biological process, are summed and presented as pie charts representing possible functional annotations for the queried protein. Although the experimental subcellular localization of a protein may not be known, and thus not annotated, CELLO can confidentially suggest a subcellular localization. CELLO2GO should be a useful tool for research involving complex subcellular systems because it combines CELLO and BLAST into one platform and its output is easily manipulated such that the user-specific questions may be readily addressed.
authors.affiliation: Department of Information Engineering and Computer Science, Feng Chia University, Taichung, Taiwan; Master's Program in Biomedical Informatics and Biomedical Engineering, Feng Chia University, Taichung, Taiwan.
authors.forename: Chin-Sheng
authors.initials: CS
authors.lastname: Yu
authors.affiliation: Department of Information Engineering and Computer Science, Feng Chia University, Taichung, Taiwan.
authors.forename: Chih-Wen
authors.initials: CW
authors.lastname: Cheng
authors.affiliation: Department of Information Engineering and Computer Science, Feng Chia University, Taichung, Taiwan.
authors.forename: Wen-Chi
authors.initials: WC
authors.lastname: Su
authors.affiliation: Department of Information Engineering and Computer Science, Feng Chia University, Taichung, Taiwan.
authors.forename: Kuei-Chung
authors.initials: KC
authors.lastname: Chang
authors.affiliation: Department of Medical Informatics, Tzu Chi University, Hualien, Taiwan.
authors.forename: Shao-Wei
authors.initials: SW
authors.lastname: Huang
authors.affiliation: Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsinchu, Taiwan; Center of Bioinformatics Research, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Jenn-Kang
authors.initials: JK
authors.lastname: Hwang
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
authors.forename: Chih-Hao
authors.initials: CH
authors.lastname: Lu
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D030541:Databases, Genetic; D066264:Datasets as Topic; D020407:Internet; D058977:Molecular Sequence Annotation; D021381:Protein Transport; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012984:Software; D014584:User-Computer Interface
chemical_list: D001426:Bacterial Proteins; D011506:Proteins