title: Antibody modified porous silicon microparticles for the selective capture of cells.
abstract: Herein, the ability of porous silicon (PSi) particles for selectively binding to specific cells is investigated. PSi microparticles with a high reflectance band in the reflectivity profile are fabricated, and subsequently passivated and modified with antibodies via the Cu(I)-catalyzed alkyne-azide cycloaddition reaction and succimidyl activation. To demonstrate the ability of the antibody-modified PSi particles to selectively bind to one cell type over others, HeLa cells were transfected with surface epitopes fused to fluorescent proteins. The antibody-functionalized PSi particles showed good selectivity for the corresponding surface protein on HeLa cells, with no significant cross-reactivity. The results are important for the application of PSi particles in cell sensing and drug delivery.
authors.affiliation: School of Chemistry, ‡The Australian Centre for NanoMedicine, §ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, ∥Centre for Vascular Research, and ⊥School of Physics, The University of New South Wales , Sydney, Australia 2052.
authors.forename: Bin
authors.initials: B
authors.lastname: Guan
authors.forename: Astrid
authors.initials: A
authors.lastname: Magenau
authors.forename: Simone
authors.initials: S
authors.lastname: Ciampi
authors.forename: Katharina
authors.initials: K
authors.lastname: Gaus
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Reece
authors.forename: J Justin
authors.initials: JJ
authors.lastname: Gooding
mesh_terms: D000480:Alkynes / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D001386:Azides / Q000737:chemistry; D061565:Cycloaddition Reaction; D049452:Green Fluorescent Proteins / Q000276:immunology / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D011990:Receptors, Transferrin / Q000378:metabolism*; D012825:Silicon / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000480:Alkynes; D000911:Antibodies, Monoclonal; D001386:Azides; D011990:Receptors, Transferrin; D049452:Green Fluorescent Proteins; D012825:Silicon