title: Development of a novel noninvasive adhesive patch test for the evaluation of pigmented lesions of the skin.
abstract: BACKGROUND
  The accurate clinical assessment of melanocytic neoplasms is a challenge for clinicians. Currently, obtaining a biopsy specimen and conducting a histologic examination is the standard of care. The incidence of melanoma in white populations is high, resulting in a large number of biopsy specimens.
  
  
  OBJECTIVE
  The objective of this study is to develop a noninvasive genomic method using mRNA to classify pigmented skin lesions as either benign or malignant.
  
  
  METHODS
  An adhesive patch method was used to obtain cells from the surface of melanocytic lesions. mRNA was extracted and a genomic signature was formulated in a training set of benign and malignant melanocytic neoplasms and subsequently tested in a validation set.
  
  
  RESULTS
  A 2-gene signature assessing the expression levels of CMIP and LINC00518 was able to differentiate melanomas from nevi in an independent validation set of 42 melanomas and 22 nevi with a sensitivity of 97.6% and specificity of 72.7%.
  
  
  CONCLUSIONS
  Larger and more diverse sets of melanomas and nevi are needed for additional validation of the molecular expression profiling in various subsets of melanocytic neoplasms.
  
  
  CONCLUSIONS
  Our data suggest that mRNA molecular signatures can serve as a highly useful noninvasive method of differentiating melanoma from nevi and decrease the number of unnecessary biopsies.
authors.affiliation: Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois; Robert H. Lurie Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, Illinois. Electronic address: Pedram.Gerami@nmff.org.
authors.forename: Pedram
authors.initials: P
authors.lastname: Gerami
authors.affiliation: DermTech International, La Jolla, California.
authors.forename: John P
authors.initials: JP
authors.lastname: Alsobrook
authors.affiliation: DermTech International, La Jolla, California.
authors.forename: Tara J
authors.initials: TJ
authors.lastname: Palmer
authors.affiliation: DermTech International, La Jolla, California.
authors.forename: Howard S
authors.initials: HS
authors.lastname: Robin
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000269:Adhesives; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D002352:Carrier Proteins / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008545:Melanoma / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics*; D008875:Middle Aged; D009508:Nevus, Pigmented / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis*; D012372:ROC Curve; D012878:Skin Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics*; D012882:Skin Tests; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000269:Adhesives; C540426:CMIP protein, human; D002352:Carrier Proteins; D012333:RNA, Messenger
keywords: dysplastic nevi; melanocytic neoplasms; melanoma; molecular signatures; nevi