title: Cytotoxic effect of icaritin and its mechanisms in inducing apoptosis in human burkitt lymphoma cell line.
abstract: Icaritin (ICT), a hydrolytic product of icariin from Epimedium genus, exhibits antitumor activities in several human solid-tumor and myeloid leukemia cells with extensive influence on various cell signal molecules, such as MAPKs being involved in cell proliferation and Bcl-2 participating in cell apoptosis. However, the effect of icaritin on Burkitt Lymphoma has not been elucidated. In the present study, we first screened the potential effect of icaritin on Burkitt lymphoma Raji and P3HR-1 cell lines and found that icaritin showed cytotoxicity in both cell lines. We further found that icaritin could significantly inhibit Raji cells proliferation with S-phase arrest of cell cycle and induced cell apoptosis accompanied by activation of caspase-8 and caspase-9 and cleavage of PARP. We also observed that icaritin was able to decrease Bcl-2 levels, thus shifting the Bcl-2/Bax ratio, and it could obviously reduce c-Myc, a specific molecular target in Burkitt lymphoma. Our findings demonstrated that icaritin showed cytotoxicity, inhibited cell growth, caused S arrest, and induced apoptosis in Burkitt lymphoma cells and provided a rationale for the further evaluation of icaritin for Burkitt lymphoma therapy.
authors.affiliation: Division of Hematology, The First Affiliated Hospital, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Zi-Jian
authors.identifier: 0000-0002-6796-4176
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Division of Nephrology, The First Affiliated Hospital, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Can
authors.initials: C
authors.lastname: Yao
authors.affiliation: Division of Hematology, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan 410011, China.
authors.forename: Su-Fang
authors.initials: SF
authors.lastname: Liu
authors.affiliation: Division of Hematology, The First Affiliated Hospital, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: Division of Hematology, The First Affiliated Hospital, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Ya-Ming
authors.initials: YM
authors.lastname: Xi
authors.affiliation: Division of Hematology, The First Affiliated Hospital, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Division of Hematology, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan 410011, China.
authors.forename: Guang-Sen
authors.initials: GS
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002051:Burkitt Lymphoma / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000008:administration & dosage*; D006801:Humans; D016896:Treatment Outcome
chemical_list: D018797:Cell Cycle Proteins; D003603:Cytotoxins; D005419:Flavonoids; C499403:icaritin