title: Analysis and pharmacokinetic study of polyphyllin H in beagle dog plasma after oral administration of Rhizoma Paridis extracts by LC-MS/MS.
abstract: A highly sensitive, rapid assay method has been developed and validated for the analysis of polyphyllin H in beagle dog plasma with liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive-ion mode. The assay procedure involves extraction of polyphyllin H and ginsenoside Re (IS) from beagle dog plasma. Chromatographic separation was carried out on an Agilent Zorbax XDB-C18 (100 × 2.1 mm, 1.8μm) column by isocratic elution with acetonitrile and water (50:50, v/v) at a flow rate of 0.25 mL/min with a total run time of 2.5 min. The MS/MS ion transitions monitored were m/z 869.60 → 869.60 for polyphyllin H and m/z 969.60 → 969.60 for IS. [corrected] Linear responses were obtained for polyphyllin H ranging from 1 to 50 ng/mL. The intra-and inter-day precisions (RSDs) <1.77 and 3.39% and the extraction recovery ranged from 91.89 to 93.33% with RSD <2.68%. Stability studies showed that polyphyllin H was stable in the preparation and analytical process. The results indicated that the validated method was successfully used to determine the concentration-time profiles of polyphyllin H.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100102, China.
authors.forename: Xingbin
authors.initials: X
authors.lastname: Yin
authors.forename: Longfei
authors.initials: L
authors.lastname: Lin
authors.forename: Mingrui
authors.initials: M
authors.lastname: Shen
authors.forename: Yujing
authors.initials: Y
authors.lastname: Zhai
authors.forename: Sali
authors.initials: S
authors.lastname: Cao
authors.forename: Jing
authors.initials: J
authors.lastname: Fu
authors.forename: Xuechun
authors.initials: X
authors.lastname: Li
authors.forename: Chunjing
authors.initials: C
authors.lastname: Yang
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Xia
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shangxin
authors.initials: S
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Bai
authors.forename: Dan
authors.initials: D
authors.lastname: Xue
authors.forename: Jian
authors.initials: J
authors.lastname: Ni
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D002867:Chromones / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D036145:Ginsenosides; D006027:Glycosides / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D019684:Magnoliopsida / Q000737:chemistry*; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002867:Chromones; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D006027:Glycosides; C000597304:polyphyllin H; C049864:ginsenoside Re
keywords: LC-MS/MS; beagle dog plasma; pharmacokinetics; polyphyllin H