title: Evaluation of ATP content in hair bulbs in human scalp.
abstract: The content of ATP in scalp hair bulbs in humans was measured in the hair roots from 15 healthy volunteers. Light and electron microscopy confirmed the presence of outer and an inner root sheaths in the root of pulled out anagen hair. Incubation of samples in buffer solution led to extraction of ATP, which was measured by the chemiluminescent method. Mechanic disintegration of hair bulbs and their freezing-defrosting did not increase ATP output. The results of microscopy indicated that ATP extraction procedure was associated with separation of the outer radical sheath from the inner one without impairing the structure of the inner sheath. The mean content of ATP was 12 ± 2 pmol per bulb. The use of pulled out hair bulbs for ATP measurements simplified the procedure as involved no surgical removal of follicles.
authors.affiliation: Laboratory of Biophysical Methods of Diagnosis, Research Institute of Physico-Chemical Medicine, Federal Medical and Biological Agency of the Russian Federation, Moscow, Russia, lemik2007@yandex.ru.
authors.forename: E V
authors.initials: EV
authors.lastname: Mikhal'chik
authors.forename: M V
authors.initials: MV
authors.lastname: Suprun
authors.forename: M V
authors.initials: MV
authors.lastname: Fedorkova
authors.forename: G A
authors.initials: GA
authors.lastname: Ibragimova
authors.forename: E I
authors.initials: EI
authors.lastname: Dmitrieva
authors.forename: V A
authors.initials: VA
authors.lastname: Lipatova
authors.forename: S I
authors.initials: SI
authors.lastname: Kutsev
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000378:metabolism; D018859:Hair Follicle / Q000378:metabolism* / Q000648:ultrastructure; D006651:Histocytochemistry; D006801:Humans; D008163:Luminescent Measurements; D008854:Microscopy, Electron; D008867:Microtomy; D012535:Scalp / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000255:Adenosine Triphosphate