title: Negative correlation of CD34+ cells with blood-brain barrier permeability following traumatic brain injury in a rat model.
abstract: OBJECTIVE
  TBI causes localized cerebral ischemia that, in turn, is accompanied by both changes in BBB permeability and recruitment of CD34(+) cells to the injured tissue. However, it remains unknown whether CD34(+) cell recruitment is linked to BBB permeability. This study is a preliminary investigation into possible correlations between CD34(+) cell recruitment and BBB permeability following TBI in a rat model.
  
  
  METHODS
  Male SD rats were subjected to mild fluid percussion injury. BBB permeability was assessed by measuring extrinsic EB dye extravasation and endogenous EBA expression at days 1, 3, 5, 7, and 12 post injury. The number of CD34(+) cells in the damaged tissue was analyzed by immunohistochemistry at each time point.
  
  
  RESULTS
  EB dye extravasation reached a peak at day 3 following TBI, while EBA expression displayed the reverse profile. Accumulation of CD34(+) cells in injured brain tissue was evident at five days post injury. It revealed a negative linear correlation between CD34(+) cell and BBB permeability.
  
  
  CONCLUSIONS
  The negative linear correlation between CD34(+) cell recruitment and BBB permeability following TBI provides a support for further study of CD34(+) cell transplantation for BBB repair after TBI.
authors.affiliation: Department of Physiology, Tianjin Medical University, Tianjin, China.
authors.forename: Xuelong
authors.initials: X
authors.lastname: Jin
authors.forename: Feifei
authors.initials: F
authors.lastname: Wang
authors.forename: Xingju
authors.initials: X
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Liang
authors.forename: Zequn
authors.initials: Z
authors.lastname: Chen
authors.forename: Junfeng
authors.initials: J
authors.lastname: He
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Jianning
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D001812:Blood-Brain Barrier / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D002199:Capillary Permeability; D004195:Disease Models, Animal; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018952:Antigens, CD34
keywords: CD34+ cells; blood-brain barrier; endothelial barrier antigen; traumatic brain injury