title: Hypoxia imaging endoscopy equipped with laser light source from preclinical live animal study to first-in-human subject research.
abstract: A goal in next-generation endoscopy is to develop functional imaging techniques to open up new opportunities for cancer diagnosis. Although spatial and temporal information on hypoxia is crucial for understanding cancer physiology and expected to be useful for cancer diagnosis, existing techniques using fluorescent indicators have limitations due to low spatial resolution and invasive administration. To overcome these problems, we developed an imaging technology based on hemoglobin oxygen saturation in both the tumor and surrounding mucosa using a laser endoscope system, and conducted the first human subject research for patients with aero-digestive tract cancer. The oxygen saturation map overlapped the images of cancerous lesions and indicated highly heterogeneous features of oxygen supply in the tumor. The hypoxic region of the tumor surface was found in both early cancer and cancer precursors. This technology illustrates a novel aspect of cancer biology as a potential biomarker and can be widely utilized in cancer diagnosis.
authors.affiliation: Department of Gastroenterology, Endoscopy Division, National Cancer Center Hospital East, Kashiwa, Chiba, Japan; Division of Science and Technology for Endoscopy and Surgery, National Cancer Center Hospital East, Kashiwa, Chiba, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kaneko
authors.affiliation: Imaging Technology Center, FUJIFILM Corporation, Kaisei, Kanagawa, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamaguchi
authors.affiliation: Imaging Technology Center, FUJIFILM Corporation, Kaisei, Kanagawa, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Saito
authors.affiliation: Department of Gastroenterology, Endoscopy Division, National Cancer Center Hospital East, Kashiwa, Chiba, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Yano
authors.affiliation: Department of Gastroenterology, Endoscopy Division, National Cancer Center Hospital East, Kashiwa, Chiba, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Oono
authors.affiliation: Department of Gastroenterology, Endoscopy Division, National Cancer Center Hospital East, Kashiwa, Chiba, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ikematsu
authors.affiliation: Clinical Trial Section, National Cancer Center, Kashiwa, Chiba, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Nomura
authors.affiliation: Clinical Trial Section, National Cancer Center, Kashiwa, Chiba, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sato
authors.affiliation: Department of Pathology, National Cancer Center Hospital East, Kashiwa, Chiba, Japan.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Kojima
authors.affiliation: Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Esumi
authors.affiliation: Department of Pathology, National Cancer Center Hospital East, Kashiwa, Chiba, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ochiai
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D004064:Digestive System / Q000473:pathology; D004724:Endoscopy; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000175:diagnosis*; D007834:Lasers; D008297:Male; D008819:Mice, Nude; D008875:Middle Aged; D010100:Oxygen / Q000378:metabolism; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012106:Research; D034421:Sus scrofa; D023041:Xenograft Model Antitumor Assays
chemical_list: D006454:Hemoglobins; D010100:Oxygen