title: Acute photo-induced toxicity and toxicokinetics of single compounds and mixtures of polycyclic aromatic hydrocarbons in zebrafish.
abstract: The present study examined photo-induced toxicity and toxicokinetics for acute exposure to selected polycyclic aromatic hydrocarbons (PAHs) in zebrafish. Photo-enhanced toxicity from co-exposure to ultraviolet (UV) radiation and PAHs enhanced the toxicity and exhibited toxic effects at PAH concentrations orders of magnitude below effects observed in the absence of UV. Because environmental exposure to PAHs is usually in the form of complex mixtures, the present study examined the photo-induced toxicity of both single compounds and mixtures of PAHs. In a sensitive larval life stage of zebrafish, acute photo-induced median lethal concentrations (LC50s) were derived for 4 PAHs (anthracene, pyrene, carbazole, and phenanthrene) to examine the hypothesis that phototoxic (anthracene and pyrene) and nonphototoxic (carbazole and phenanthrene) pathways of mixtures could be predicted from single exposures. Anthracene and pyrene were phototoxic as predicted; however, carbazole exhibited moderate photo-induced toxicity and phenanthrene exhibited weak photo-induced toxicity. The toxicity of each chemical alone was used to compare the toxicity of mixtures in binary, tertiary, and quaternary combinations of these PAHs, and a predictive model for environmental mixtures was generated. The results indicated that the acute toxicity of PAH mixtures was additive in phototoxic scenarios, regardless of the magnitude of photo-enhancement. Based on PAH concentrations found in water and circumstances of high UV dose to aquatic systems, there exists potential risk of photo-induced toxicity to aquatic organisms.
authors.affiliation: Miami University, Oxford, Ohio, USA; Toxicology Excellence for Risk Assessment (TERA), Cincinnati, Ohio, USA.
authors.forename: Alison M
authors.initials: AM
authors.lastname: Willis
authors.forename: James T
authors.initials: JT
authors.lastname: Oris
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000633:toxicity; D045424:Complex Mixtures / Q000633:toxicity; D007928:Lethal Dose 50; D010616:Phenanthrenes / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D011721:Pyrenes / Q000633:toxicity; D014466:Ultraviolet Rays; D015027:Zebrafish / Q000502:physiology*
chemical_list: D000873:Anthracenes; D045424:Complex Mixtures; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C031181:phenanthrene; C030984:pyrene; C034020:anthracene
keywords: Median lethal concentration (LC50); Mixtures additivity; Multiple stressors; Phototoxicity; Polycyclic aromatic hydrocarbon