title: Liver regeneration with l-glutamine supplemented diet: experimental study in rats.
abstract: OBJECTIVE
  To assess liver regeneration in rats after 60% hepatectomy with and without supplementation of L-glutamine through liver weight changes, laboratory parameters and histological study.
  
  
  METHODS
  36 male rats were divided into two groups: glutamine group and control group. Each group was subdivided into three subgroups, with death in 24h, 72h and seven days. The glutamine group received water and standard diet supplemented with L-glutamine, and the control recieved 0.9% saline. In all subgroups analysis of liver regeneration was made by the Kwon formula, study of liver function (AST, ALT, GGT, total bilirubin, indirect and indirect bilirubin and albumin) and analysis of cell mitosis by hematoxylin-eosin.
  
  
  RESULTS
  In both groups there was liver regeneration by weight gain. Gamma-GT increased significantly in the control group (p < 0.05); albumin increased in the glutamine group. The other indicators of liver function showed no significant differences. Histological analysis at 72h showed a higher number of mitoses in the glutamine group, with no differences in other subgroups.
  
  
  CONCLUSIONS
  Diet supplementation with L glutamine is beneficial for liver regeneration.
authors.affiliation: Medical Research Institute, Evangelical Faculty of Paraná, Evangelical University Hospital.
authors.forename: Cibelle Ribeiro
authors.initials: CR
authors.lastname: Magalhães
authors.affiliation: Medical Research Institute, Evangelical Faculty of Paraná, Evangelical University Hospital.
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Malafaia
authors.affiliation: Department of Surgery, Universidade Federal do Maranhão, São Luís, MA, Brasil.
authors.forename: Orlando Jorge Martins
authors.initials: OJ
authors.lastname: Torres
authors.affiliation: Medical Research Institute, Evangelical Faculty of Paraná, Evangelical University Hospital.
authors.forename: Luciane Bugmann
authors.initials: LB
authors.lastname: Moreira
authors.affiliation: Medical Research Institute, Evangelical Faculty of Paraná, Evangelical University Hospital.
authors.forename: Silvia Carmen da Silva Gonçalves
authors.initials: SC
authors.lastname: Tefil
authors.affiliation: Hospital Universitário Evangélico de Curitiba, Faculdade Evangélica do Paraná, PR, Brasil.
authors.forename: Matheus da Rocha
authors.initials: Mda R
authors.lastname: Pinherio
authors.affiliation: Hospital Universitário Evangélico de Curitiba, Faculdade Evangélica do Paraná, PR, Brasil.
authors.forename: Bruna Ayumi
authors.initials: BA
authors.lastname: Harada
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D005973:Glutamine / Q000494:pharmacology*; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005973:Glutamine