title: Engineered multifunctional nanomaterials for multimodal imaging of retinoblastoma cells in vitro.
abstract: Multifunctional nanoparticles are next generation materials that can be simultaneously used for imaging, diagnosis, and delivery of drugs. However, materials intended for cancer diagnosis need to be investigated for its cell uptake, toxicity, and effectiveness. In the current work, we have synthesized fluorescent iron oxide nanoparticles and evaluated its efficacy against retinoblastoma cell imaging. The iron oxide nanoparticles were synthesized and stabilized using oleic acid. Sulforhodamine B was adsorbed onto albumin over the oleic acid-capped iron oxide nanoparticles. Our results demonstrated good cell uptake in a time-dependent manner and nanoparticles were found to localize in the cytosol. Further, the nanoparticles exhibited excellent negative contrast in magnetic resonance imaging (MRI) experiments and with no cytoxicity (5-100 μg/mL iron oxide nanoparticles) to both normal as well as cancer cells demonstrating its biocompatibility. Thus, this novel material integrates the ability to image tissues with high sensitivity by MRI and specifically visualize Y79 retinoblastoma cells by fluorescence imaging with no toxicity.
authors.affiliation: a Center for Nanotechnology & Advanced Biomaterials (CeNTAB), School of Chemical & Biotechnology , SASTRA University , Thanjavur 613401 , India.
authors.forename: Leela Raghava
authors.initials: LR
authors.lastname: Jaidev
authors.forename: Dhiraj Vasanth
authors.initials: DV
authors.lastname: Bhavsar
authors.forename: Uma
authors.initials: U
authors.lastname: Sharma
authors.forename: Naranamanglam R
authors.initials: NR
authors.lastname: Jagannathan
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Krishnan
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethuraman
mesh_terms: D000362:Agar; D000418:Albumins / Q000737:chemistry; D000588:Amines; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D060328:Magnetic Phenomena; D008279:Magnetic Resonance Imaging / Q000295:instrumentation; D008422:Materials Testing; D008856:Microscopy, Fluorescence; D064847:Multimodal Imaging; D053758:Nanoparticles / Q000737:chemistry; D019301:Oleic Acid / Q000737:chemistry; D019047:Phantoms, Imaging; D012175:Retinoblastoma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D012235:Rhodamines / Q000737:chemistry
chemical_list: D000418:Albumins; D000588:Amines; D003287:Contrast Media; D005290:Ferric Compounds; D005456:Fluorescent Dyes; C493330:Red DND-99; D012235:Rhodamines; C000499:ferric oxide; C022027:lissamine rhodamine B; D019301:Oleic Acid; D000362:Agar
keywords: cancer imaging; fluorescent imaging; fluorescent nanoparticles; iron oxide; multifunctional nanocarriers