title: Distance-determined sensitivity in attenuated total reflection-surface enhanced infrared absorption spectroscopy: aptamer-antigen compared to antibody-antigen.
abstract: Distance-dependent signal intensity in immunoassay by attenuated total reflection-surface enhanced infrared absorption spectroscopy is demonstrated by controlling the distance of target proteins away from the enhancement substrate. Based on this optical near-field effect, sensitive detection of protein molecules with a detection limit of 0.6 nM and investigation of the kinetics and thermodynamics of protein-aptamer/antibody interactions can be achieved.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China. xhxia@nju.edu.cn.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Bao
authors.forename: Zhen-Dong
authors.initials: ZD
authors.lastname: Yan
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Kang
authors.initials: K
authors.lastname: Wang
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Xia
authors.forename: Zhen-Lin
authors.initials: ZL
authors.lastname: Wang
mesh_terms: D000906:Antibodies / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D052158:Aptamers, Peptide / Q000276:immunology*; D007118:Immunoassay / Q000379:methods*; D019041:L-Selectin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013816:Thermodynamics
chemical_list: D000906:Antibodies; D000941:Antigens; D052158:Aptamers, Peptide; D011506:Proteins; D019041:L-Selectin