title: Developing Exon-Primed Intron-Crossing (EPIC) markers for population genetic studies in three Aedes disease vectors.
abstract: Aedes aegypti, Aedes notoscriptus, and Aedes albopictus are important vectors of many arboviruses implicated in human disease such as dengue fever. Genetic markers applied across vector species can provide important information on population structure, gene flow, insecticide resistance, and taxonomy, however, robust microsatellite markers have proven difficult to develop in these species and mosquitoes generally. Here we consider the utility and transferability of 15 Ribosome protein (Rp) Exon-Primed Intron-Crossing (EPIC) markers for population genetic studies in these 3 Aedes species. Rp EPIC markers designed for Ae. aegypti also successfully amplified populations of the sister species, Ae. albopictus, as well as the distantly related species, Ae. notoscriptus. High SNP and good indel diversity in sequenced alleles plus support for amplification of the same regions across populations and species were additional benefits of these markers. These findings point to the general value of EPIC markers in mosquito population studies.
authors.affiliation: Department of Genetics, Bio21 Institute, the University of Melbourne, Victoria, 3010, Australia.
authors.forename: Vanessa Linley
authors.initials: VL
authors.lastname: White
authors.affiliation: Department of Genetics, Bio21 Institute, the University of Melbourne, Victoria, 3010, Australia.
authors.forename: Nancy Margaret
authors.initials: NM
authors.lastname: Endersby
authors.affiliation: Department of Genetics, Bio21 Institute, the University of Melbourne, Victoria, 3010, Australia.
authors.forename: Janice
authors.initials: J
authors.lastname: Chan
authors.affiliation: Department of Genetics, Bio21 Institute, the University of Melbourne, Victoria, 3010, Australia.
authors.forename: Ary Anthony
authors.initials: AA
authors.lastname: Hoffmann
authors.affiliation: Department of Genetics, Bio21 Institute, the University of Melbourne, Victoria, 3010, Australia.
authors.forename: Andrew Raymond
authors.initials: AR
authors.lastname: Weeks
mesh_terms: D000330:Aedes / Q000235:genetics*; D000818:Animals; D005091:Exons; D005819:Genetic Markers; D005828:Genetics, Population; D007303:Insect Vectors / Q000235:genetics; D007438:Introns; D012269:Ribosomal Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers; D012269:Ribosomal Proteins
keywords: Aedes aegypti; Aedes albopictus; Aedes notoscriptus; Exon-Primed Intron-Crossing (EPIC); Ribosome protein (Rp); SNPs; indels