title: Preparing a cell for nuclear envelope breakdown: Spatio-temporal control of phosphorylation during mitotic entry.
abstract: Chromosome segregation requires the ordered separation of the newly replicated chromosomes between the two daughter cells. In most cells, this requires nuclear envelope (NE) disassembly during mitotic entry and its reformation at mitotic exit. Nuclear envelope breakdown (NEB) results in the mixture of two cellular compartments. This process is controlled through phosphorylation of multiple targets by cyclin-dependent kinase 1 (Cdk1)-cyclin B complexes as well as other mitotic enzymes. Experimental evidence also suggests that nucleo-cytoplasmic transport of critical cell cycle regulators such as Cdk1-cyclin B complexes or Greatwall, a kinase responsible for the inactivation of PP2A phosphatases, plays a major role in maintaining the boost of mitotic phosphorylation thus preventing the potential mitotic collapse derived from NEB. These data suggest the relevance of nucleo-cytoplasmic transport not only to communicate cytoplasmic and nuclear compartments during interphase, but also to prepare cells for the mixture of these two compartments during mitosis.
authors.affiliation: Cell Division and Cancer Group, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Alvarez-Fernández
authors.forename: Marcos
authors.initials: M
authors.lastname: Malumbres
mesh_terms: D000818:Animals; D020090:Chromosome Segregation; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000502:physiology; D008938:Mitosis; D009685:Nuclear Envelope / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000502:physiology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000502:physiology; D021381:Protein Transport
chemical_list: D008869:Microtubule-Associated Proteins; C555237:MASTL protein, human; D017346:Protein Serine-Threonine Kinases; D010744:Phosphoric Monoester Hydrolases
keywords: Cdk; Greatwall; Mastl; PP2A phosphatase; cell division cycle; cyclin; mitosis; phosphorylation