title: The study of zinc ions binding to casein.
abstract: The presented research was focused on physicochemical study of casein properties and the kinetics of zinc ions binding to the protein. Moreover, a fast and simple method of casein extraction from cow's milk has been proposed. Casein isoforms, zeta potential (ζ) and particle size of the separated caseins were characterized with the use of capillary electrophoresis, zeta potential analysis and field flow fractionation (FFF) technique, respectively. The kinetics of the metal-binding process was investigated in batch adsorption experiments. Intraparticle diffusion model, first-order and zero-order kinetic models were applied to test the kinetic experimental data. Analysis of changes in infrared bands registered for casein before and after zinc binding was also performed. The obtained results showed that the kinetic process of zinc binding to casein is not homogeneous but is expressed with an initial rapid stage with about 70% of zinc ions immobilized by casein and with a much slower second step. Maximum amount of bound zinc in the experimental conditions was 30.04mgZn/g casein.
authors.affiliation: Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 7 Gagarina Str., 87-100 Torun, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Pomastowski
authors.affiliation: Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 7 Gagarina Str., 87-100 Torun, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Sprynskyy
authors.affiliation: Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 7 Gagarina Str., 87-100 Torun, Poland. Electronic address: bbusz@chem.umk.pl.
authors.forename: B
authors.initials: B
authors.lastname: Buszewski
mesh_terms: D000818:Animals; D002364:Caseins / Q000378:metabolism*; D002417:Cattle; D004586:Electrophoresis; D031521:Fractionation, Field Flow; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D010316:Particle Size; D013055:Spectrophotometry, Infrared; D055672:Static Electricity; D015032:Zinc / Q000378:metabolism*
chemical_list: D002364:Caseins; D007477:Ions; D015032:Zinc
keywords: Binding kinetics; Casein; Metalloproteins; Zinc