title: Fibre-specific responses to endurance and low volume high intensity interval training: striking similarities in acute and chronic adaptation.
abstract: The current study involved the completion of two distinct experiments. Experiment 1 compared fibre specific and whole muscle responses to acute bouts of either low-volume high-intensity interval training (LV-HIT) or moderate-intensity continuous endurance exercise (END) in a randomized crossover design. Experiment 2 examined the impact of a six-week training intervention (END or LV-HIT; 4 days/week), on whole body and skeletal muscle fibre specific markers of aerobic and anaerobic capacity. Six recreationally active men (Age: 20.7 ± 3.8 yrs; VO2peak: 51.9 ± 5.1 mL/kg/min) reported to the lab on two separate occasions for experiment 1. Following a muscle biopsy taken in a fasted state, participants completed an acute bout of each exercise protocol (LV-HIT: 8, 20-second intervals at ∼ 170% of VO2peak separated by 10 seconds of rest; END: 30 minutes at ∼ 65% of VO2peak), immediately followed by a muscle biopsy. Glycogen content of type I and IIA fibres was significantly (p<0.05) reduced, while p-ACC was significantly increased (p<0.05) following both protocols. Nineteen recreationally active males (n = 16) and females (n = 3) were VO2peak-matched and assigned to either the LV-HIT (n = 10; 21 ± 2 yrs) or END (n = 9; 20.7 ± 3.8 yrs) group for experiment 2. After 6 weeks, both training protocols induced comparable increases in aerobic capacity (END: Pre: 48.3 ± 6.0, Mid: 51.8 ± 6.0, Post: 55.0 ± 6.3 mL/kg/min LV-HIT: Pre: 47.9 ± 8.1, Mid: 50.4 ± 7.4, Post: 54.7 ± 7.6 mL/kg/min), fibre-type specific oxidative and glycolytic capacity, glycogen and IMTG stores, and whole-muscle capillary density. Interestingly, only LV-HIT induced greater improvements in anaerobic performance and estimated whole-muscle glycolytic capacity. These results suggest that 30 minutes of END exercise at ∼ 65% VO2peak or 4 minutes of LV-HIT at ∼ 170% VO2peak induce comparable changes in the intra-myocellular environment (glycogen content and signaling activation); correspondingly, training-induced adaptations resulting for these protocols, and other HIT and END protocols are strikingly similar.
authors.affiliation: School of Kinesiology and Health Studies, Queen's University, Kingston, Ontario, Canada.
authors.forename: Trisha D
authors.initials: TD
authors.lastname: Scribbans
authors.affiliation: School of Kinesiology and Health Studies, Queen's University, Kingston, Ontario, Canada.
authors.forename: Brittany A
authors.initials: BA
authors.lastname: Edgett
authors.affiliation: Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada.
authors.forename: Kira
authors.initials: K
authors.lastname: Vorobej
authors.affiliation: Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada.
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Mitchell
authors.affiliation: Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Sophie D
authors.initials: SD
authors.lastname: Joanisse
authors.affiliation: School of Kinesiology and Health Studies, Queen's University, Kingston, Ontario, Canada.
authors.forename: Jennifer B L
authors.initials: JB
authors.lastname: Matusiak
authors.affiliation: Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Gianni
authors.initials: G
authors.lastname: Parise
authors.affiliation: Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada.
authors.forename: Joe
authors.initials: J
authors.lastname: Quadrilatero
authors.affiliation: School of Kinesiology and Health Studies, Queen's University, Kingston, Ontario, Canada.
authors.forename: Brendon J
authors.initials: BJ
authors.lastname: Gurd
mesh_terms: D000222:Adaptation, Physiological; D000293:Adolescent; D015444:Exercise; D005080:Exercise Test; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000502:physiology; D010101:Oxygen Consumption; D010807:Physical Endurance; D055815:Young Adult
chemical_list: D005947:Glucose