title: A Platform for the Detection of Trypanosomes via Selective Small Molecule Recognition.
abstract: Trypanothione (TSH2), a metabolite unique to trypanosomal parasites, was evaluated as a potential biomarker for trypanosomal infection using fluorescence as the means of detection. Fluoroescein arsenical helix binder (FLASH) was prepared and used to detect TSH2. Since it has low background fluorescence and forms a highly emissive complex with TSH2, it can be used to detect low micromolar concentrations of TSH2 in serum. The large dynamic range of FLASH and its selectivity for detection of the dithiol metabolite indicate that arsenical probes may offer a promising new platform for the diagnosis of trypanosomal infection.
authors.affiliation: Center for Infectious Disease and Biodefense, SRI International , 333 Ravenswood Avenue, Menlo Park, California 94025, United States.
authors.forename: Ellen D
authors.initials: ED
authors.lastname: Beaulieu
authors.affiliation: Center for Infectious Disease and Biodefense, SRI International , 333 Ravenswood Avenue, Menlo Park, California 94025, United States.
authors.forename: Lori L
authors.initials: LL
authors.lastname: Olson
authors.affiliation: Center for Infectious Disease and Biodefense, SRI International , 333 Ravenswood Avenue, Menlo Park, California 94025, United States.
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Tanga
keywords: African sleeping sickness; Chagas' disease; Trypanosomes; diagnostic; fluorescein arsenical helix binder (FLASH); human African trypanosomiasis; leishmaniasis; trypanothione