title: Lycopene and resveratrol improve post-thaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity.
abstract: We focussed on evaluating the protective effect of lycopene and resveratrol on post-thaw bull sperm and oxidative stress parameters. Nine ejaculates for each bull were used in the study. Each ejaculate, splitted into three equal aliquots and diluted at 37 °C with base extenders containing lycopene (1 × 10(-3)  g ml(-1) ) and resveratrol (1 mm), and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The supplementation of the semen extender with lycopene and resveratrol increased the percentages of post-thawed computer-assisted sperm analysis (CASA) motility (55.8 ± 3.8 and 61.9 ± 4.0%) and progressive motility (38 ± 2.4 and 37 ± 8.8), compared with the controls (50.7 ± 2.65 and 33.3 ± 3.74%, respectively, P < 0.05). Resveratrol provided a higher ALH (4.3 ± 0.1), in comparison with the control (3.9 ± 0.3, P < 0.05). The supplementation of the semen extender with lycopene and resveratrol produced a higher mitochondrial activity (24.6 ± 2.9 and 30.1 ± 6.5% respectively), compared with that of the control (11.8 ± 9.5%, P < 0.05). It was determined that both antioxidants resulted in a lower percentage of sperm with damaged DNA than that of the control (P < 0.05). Sperm motion characteristics except for ALH, acrosome integrity, sperm viability and oxidative stress parameters were not affected by the adding of lycopene and resveratrol.
authors.affiliation: Department of Reproduction and Artificial Insemination, Veterinary Faculty, Selcuk University, Konya, Turkey.
authors.forename: M N
authors.initials: MN
authors.lastname: Bucak
authors.forename: M B
authors.initials: MB
authors.lastname: Ataman
authors.forename: N
authors.initials: N
authors.lastname: Başpınar
authors.forename: O
authors.initials: O
authors.lastname: Uysal
authors.forename: M
authors.initials: M
authors.lastname: Taşpınar
authors.forename: A
authors.initials: A
authors.lastname: Bilgili
authors.forename: C
authors.initials: C
authors.lastname: Öztürk
authors.forename: Ş
authors.initials: Ş
authors.lastname: Güngör
authors.forename: M E
authors.initials: ME
authors.lastname: İnanç
authors.forename: E
authors.initials: E
authors.lastname: Akal
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002338:Carotenoids / Q000494:pharmacology*; D002417:Cattle; D015925:Cryopreservation / Q000379:methods*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects; D000077276:Lycopene; D008297:Male; D008928:Mitochondria / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D000077185:Resveratrol; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D002338:Carotenoids; D004247:DNA; D000077185:Resveratrol; D000077276:Lycopene
keywords: DNA damage; antioxidants; cryopreservation; oxidative stress; sperm