title: [Dengue vector control using ether fractions from two plants (Asteraceae) as larvicide].
abstract: OBJECTIVE
  Evaluating the larvicidal activity of two ether factions from Asteraceae (the aster, daisy or sunflower family, i.e. Heli opsisoppositifolia (L.) Druce (oxeye, sunflower-like) and Jaegeria hirta (Lag.) Less (weed-like)) on Aedes (Stegomyia) aegypti (L.) final third instar or initial fourth instar larvae near the town of Armenia in the Quindío Department in Colombia.
  
  
  METHODS
  H. oppositifolia and J. hirta plants were collected and submitted to phytochemical analysis. Ether fractions were prepared form both species to assess 11 concentrations for determining LC50, LC90 and LC95 lethal concentrations after 24 and 48 h. The LC50, LC90 and LC95 results were used to create a mathematical model for describing lethal population-concentration dynamics.
  
  
  RESULTS
  Phytochemical analysis identified tannins, flavonoids, quinones, cardiac glycosides, sterols, lactones, terpenes, courmarins and alkaloids in H. oppositifolia and J. hirta. LC after 48 h regarding J. hirta (LC50 24, LC90 70 and LC95 93 ppm) was lower than those for H. oppositifolia (LC50 39, LC90 77 and LC95 94 ppm). A factorial ANOVA test confirmed this trend: 66 %, F=18.5 and p<0.05 for J. hirta and 34 %, F=18.5 and p<0.05 for H. oppositifolia. The mathematical simulation model suggested that using LC50 every 15 days and LC90 and LC95 every 30 days from either of these species led to the same response compared to using LC90 and LC95 every 30 days or LC50 every 15 days.
  
  
  CONCLUSIONS
  Both species had a larvicidal effect. However, J. Hirta turned out to be more promising as an eventual bioinsecticide for controlling A. Aegypti immature states.
authors.affiliation: Universidad del Quindío, Colombia, iduarte@uniquindio.edu.co.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Álvarez-Londoño
authors.affiliation: Universidad del Quindío, Colombia, iduarte@uniquindio.edu.co.
authors.forename: Irene
authors.initials: I
authors.lastname: Duarte-Gandica
authors.affiliation: Departamento de Zoologia, Setor de Ciências Biológicas, Universidade Federal do Paraná, Paraná
authors.forename: Oscar A
authors.initials: OA
authors.lastname: Aguirre-Obando
authors.affiliation: Universidad del Quindío, Colombia, iduarte@uniquindio.edu.co.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Jiménez-Montoya
mesh_terms: D000330:Aedes; D000818:Animals; D019659:Asteraceae; D003105:Colombia; D003715:Dengue / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D004986:Ether; D007303:Insect Vectors; D007814:Larva / Q000187:drug effects; D009032:Mosquito Control / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology; D013997:Time Factors
chemical_list: D010936:Plant Extracts; D004986:Ether