title: Noninvasive and label-free determination of virus infected cells by Raman spectroscopy.
abstract: The present study demonstrates that Raman spectroscopy is a powerful tool for the detection of virus-infected cells. Adenovirus infection of human embryonic kidney 293 cells was successfully detected at 12, 24, and 48 h after initiating the infection. The score plot of principal component analysis discriminated the spectra of the infected cells from those of the control cells. The viral infection was confirmed by the conventional immunostaining method performed 24 h after the infection. The newly developed method provides a fast and label-free means for the detection of virus-infected cells.
authors.affiliation: Kwansei Gakuin University, School of Science and Technology, Department of Bioscience, 2-1 Gakuen, Sanda, Hyogo 669-1337 Japan.
authors.forename: Kamila
authors.initials: K
authors.lastname: Moor
authors.affiliation: Kwansei Gakuin University, School of Science and Technology, Department of Bioscience, 2-1 Gakuen, Sanda, Hyogo 669-1337 Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ohtani
authors.affiliation: Kazakh National Agrarian University, Kazakhstan-Japan Innovation Centre, 8, Abai Street, Almaty 050010, Kazakhstan.
authors.forename: Diyas
authors.initials: D
authors.lastname: Myrzakozha
authors.affiliation: Kazakh National Agrarian University, Kazakhstan-Japan Innovation Centre, 8, Abai Street, Almaty 050010, Kazakhstan.
authors.forename: Orik
authors.initials: O
authors.lastname: Zhanserkenova
authors.affiliation: Kwansei Gakuin University, School of Science and Technology, Department of Bioscience, 2-1 Gakuen, Sanda, Hyogo 669-1337 Japan.
authors.forename: Bibin B
authors.initials: BB
authors.lastname: Andriana
authors.affiliation: Kwansei Gakuin University, School of Science and Technology, Department of Bioscience, 2-1 Gakuen, Sanda, Hyogo 669-1337 Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Sato
mesh_terms: D000256:Adenoviridae; D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000473:pathology; D057809:HEK293 Cells / Q000821:virology*; D006801:Humans; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors