title: The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination.
abstract: Fermentative hydrogen production from wastes has many advantages compared to various chemical methods. Methodology for characterizing the hydrogen-producing activity of anaerobic mixed cultures is essential for monitoring reactor operation in fermentative hydrogen production, however there is lack of such kind of standardized methodologies. In the present study, a new index, i.e., the maximum specific hydrogen-producing activity (SHAm) of anaerobic mixed cultures, was proposed, and consequently a reliable and simple method, named SHAm test, was developed to determine it. Furthermore, the influences of various parameters on the SHAm value determination of anaerobic mixed cultures were evaluated. Additionally, this SHAm assay was tested for different types of substrates and bacterial inocula. Our results demonstrate that this novel SHAm assay was a rapid, accurate and simple methodology for determining the hydrogen-producing activity of anaerobic mixed cultures. Thus, application of this approach is beneficial to establishing a stable anaerobic hydrogen-producing system.
authors.affiliation: Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China.
authors.forename: Hou-Yun
authors.initials: HY
authors.lastname: Yang
authors.affiliation: Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China.
authors.forename: Ya-Zhou
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China.
authors.forename: Chuan-Shu
authors.initials: CS
authors.lastname: He
authors.affiliation: Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China.
authors.forename: Quan-Bao
authors.initials: QB
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China.
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Yu
mesh_terms: D000693:Anaerobiosis / Q000502:physiology*; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D005285:Fermentation / Q000502:physiology; D006859:Hydrogen / Q000378:metabolism*
chemical_list: D006859:Hydrogen