title: Quantitative mass spectrometric analysis of glycoproteins combined with enrichment methods.
abstract: Mass spectrometry (MS) has been a core technology for high sensitive and high-throughput analysis of the enriched glycoproteome in aspects of quantitative assays as well as qualitative profiling of glycoproteins. Because it has been widely recognized that aberrant glycosylation in a glycoprotein may involve in progression of a certain disease, the development of efficient analysis tool for the aberrant glycoproteins is very important for deep understanding about pathological function of the glycoprotein and new biomarker development. This review first describes the protein glycosylation-targeting enrichment technologies mainly employing solid-phase extraction methods such as hydrizide-capturing, lectin-specific capturing, and affinity separation techniques based on porous graphitized carbon, hydrophilic interaction chromatography, or immobilized boronic acid. Second, MS-based quantitative analysis strategies coupled with the protein glycosylation-targeting enrichment technologies, by using a label-free MS, stable isotope-labeling, or targeted multiple reaction monitoring (MRM) MS, are summarized with recent published studies.
authors.affiliation: Division of Mass Spectrometry, Korea Basic Science Institute, Cheongwon-Gun, 363-883, Republic of Korea.
authors.forename: Yeong Hee
authors.initials: YH
authors.lastname: Ahn
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Jong Shin
authors.initials: JS
authors.lastname: Yoo
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001897:Boronic Acids / Q000737:chemistry; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods; D006023:Glycoproteins / Q000302:isolation & purification*; D006031:Glycosylation; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods; D037102:Lectins / Q000737:chemistry; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D015415:Biomarkers; D001897:Boronic Acids; D006023:Glycoproteins; D006834:Hydrazines; D037102:Lectins
keywords: affinity enrichment; hydrazide; lectin; multiple reaction monitoring; protein glycosylation; quantitative mass spectrometry; stable isotope labeling