title: Visualization of G3BP stress granules dynamics in live primary cells.
abstract: SGs can be visualized in cells by immunostaining of specific protein components or polyA+ mRNAs. SGs are highly dynamic and the study of their assembly and fate is important to understand the cellular response to stress. The deficiency in key factors of SGs like G3BP (RasGAP SH3 domain Binding Protein) leads to developmental defects in mice and alterations of the Central Nervous System. To study the dynamics of SGs in cells from an organism, one can culture primary cells and follow the localization of a transfected tagged component of SGs. We describe time-lapse experiment to observe G3BP1-containing SGs in Mouse Embryonic Fibroblasts (MEFs). This technique can also be used to study G3BP-containing SGs in live neurons, which is crucial as it was recently shown that these SGs are formed at the onset of neurodegenerative diseases like Alzheimer's disease. This approach can be adapted to any other cellular body and granule protein component, and performed with transgenic animals, allowing the live study of granules dynamics for example in the absence of a specific factor of these granules.
authors.affiliation: Institut de Génétique Moléculaire de Montpellier, CNRS UMR 5535; martinsophie.univ@yahoo.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Martin
authors.affiliation: Institut de Génétique Moléculaire de Montpellier, CNRS UMR 5535.
authors.forename: Jamal
authors.initials: J
authors.lastname: Tazi
mesh_terms: D000818:Animals; D018053:Arsenites; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D004265:DNA Helicases; D005347:Fibroblasts; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000075223:Poly-ADP-Ribose Binding Proteins; D020365:RNA Helicases; D000067776:RNA Recognition Motif Proteins; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000032:analysis / Q000378:metabolism; D013312:Stress, Physiological / Q000502:physiology*; D014162:Transfection
chemical_list: D018053:Arsenites; D002352:Carrier Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D000067776:RNA Recognition Motif Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D004265:DNA Helicases; C492055:G3bp1 protein, mouse; D020365:RNA Helicases; C015001:arsenite