title: Adrenomedullin increases the short-circuit current in the mouse seminal vesicle: actions on chloride secretion.
abstract: Adrenomedullin (ADM) may regulate seminal vesicle fluid secretion, and this may affect sperm quality. In this study, we investigated the effect of ADM on chloride secretion in the mouse seminal vesicle. The presence of ADM in mouse seminal vesicle was confirmed using immunostaining, and the molecular species was determined using gel filtration chromatography coupled with enzyme-linked assay for ADM. The effects of ADM on chloride secretion were studied by short-circuit current technique in a whole-mount preparation of mouse seminal vesicle in an Ussing chamber. The effects of specific ADM and calcitonin gene-related peptide (CGRP) receptor antagonists were investigated. Whether the ADM effect depended on the cAMP- and/or calcium-activated chloride channel was also studied using specific chloride channel blockers. The results showed that ADM was present in seminal vesicle epithelial cells. The major molecular species was precursor in the mouse seminal vesicle. ADM increased short-circuit current through the calcium-activated chloride channel in mouse seminal vesicle, and CGRP receptor was involved. We conclude that ADM may regulate chloride and fluid secretion from the seminal vesicle, which may affect the composition of the seminal plasma bathing the sperm and, hence, fertility.
authors.affiliation: Department of Anatomy, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China Department of Physiology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China Department of Obstetrics and Gynecology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China.
authors.forename: S B
authors.initials: SB
authors.lastname: Liao
authors.affiliation: Department of Physiology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China Centre of Heart, Brain, Hormone, and Healthy Aging, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China ckingho@hku.hk.
authors.forename: K H
authors.initials: KH
authors.lastname: Cheung
authors.affiliation: Department of Anatomy, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China Centre of Growth, Reproduction, and Development, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China.
authors.forename: W S
authors.initials: WS
authors.lastname: O
authors.affiliation: Department of Physiology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China Centre of Heart, Brain, Hormone, and Healthy Aging, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China.
authors.forename: Fai
authors.initials: F
authors.lastname: Tang
mesh_terms: D053607:Adrenomedullin / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002227:Carbazoles / Q000494:pharmacology; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D055724:Electrophysiological Phenomena / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011758:Pyrroles / Q000494:pharmacology; D012669:Seminal Vesicles / Q000378:metabolism*; D062367:ortho-Aminobenzoates / Q000494:pharmacology
chemical_list: D002227:Carbazoles; D018118:Chloride Channels; D002712:Chlorides; D011758:Pyrroles; D062367:ortho-Aminobenzoates; D053607:Adrenomedullin; C057416:KT 5720; C001209:fenamic acid; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: adrenomedullin; chloride channel; epithelial transport; guinea pigs; ion channels; male reproductive tract; mice; rodents (rats; seminal vesicles; voles)