title: Multiwalled carbon nanotube-modified poly(D,L-lactide-co-glycolide) scaffolds for dendritic cell load.
abstract: Poly(D,L-lactide-co-glycolide) (PLGA) is widely used in a variety of tissue engineering and drug delivery applications due to its biodegradability and biocompatibility. But PLGA surfaces are usually hydrophobic which limited the loading and seeding capacities for cells, especially semiadherent immune cells. In this paper we described an attempt to improve the hydrophilicity and surface architecture for accommodating dendritic cells (DCs) that are widely used as professional antigen presenting cells in immune therapy of cancer and other diseases. The 3D porous PLGA scaffold was made by solvent casting/salt leaching of PLGA blended with surface functionalized multiwalled carbon nanotubes (F-MWCNTs). The incorporation and dispersion of F-MWCNT in the scaffold structures resulted in not only improved surface hydrophilicity but also nanoscale surface structure that would provide a preferable microenvironment for DCs attachment. We think such a scaffold material may be more desirable for immune cell delivery for immunotherapy.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
authors.forename: Yanzhu
authors.initials: Y
authors.lastname: Yang
authors.forename: Sanyuan
authors.initials: S
authors.lastname: Shi
authors.forename: Qian
authors.initials: Q
authors.lastname: Ding
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Jinliang
authors.initials: J
authors.lastname: Peng
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology; D002244:Carbon / Q000737:chemistry; D002448:Cell Adhesion; D003713:Dendritic Cells / Q000166:cytology*; D007167:Immunotherapy / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D051379:Mice; D008810:Mice, Inbred C57BL; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D000941:Antigens; D001672:Biocompatible Materials; D037742:Nanotubes, Carbon; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002244:Carbon
keywords: PLGA scaffold; cell adhesion; dendritic cells; functionalized multiwalled carbon nanotubes; immunotherapy