title: Chromogranins can be measured in samples from cats and dogs.
abstract: BACKGROUND
  Methods for objective evaluation of stress in animals are important, but clinically difficult. An alternative method to study the sympathetic activity may be to investigate Chromogranin A (CGA), Chromogranin B (CGB) and Secretogranin II (SG2). The aim of this study was to investigate the cross-reactivity of CGA, CGB and SG2 between man, cat and dog and to explore possibilities to measure these proteins in samples from cats and dogs.
  
  
  RESULTS
  Adrenal gland extracts from feline and canine species were measured by region-specific radioimmunoassays in different dilution steps to explore possible inter species cross reactivity. High cross reactivity was found for cats in the CGA17-38, CGA324-337, CGA361-372, CGB and SG2 assays. High cross reactivity was found for dogs in the CGA17-38, CGA361-372, CGB and SN assays. The method measuring the intact CGA was not useful for measurements in cats and dogs.
  
  
  CONCLUSIONS
  Region-specific assays measuring defined parts of CGA, CGB and SG2 can be used for measurements in samples from cats and dogs. These results are promising and will allow for further studies of these proteins as possible clinical biomarkers in cats and dogs.
authors.affiliation: Department of Medical Sciences, Uppsala University, SE-751 85 Uppsala, Sweden. Mats.Stridsberg@medsci.uu.se.
authors.forename: Mats
authors.initials: M
authors.lastname: Stridsberg
authors.forename: Ann
authors.initials: A
authors.lastname: Pettersson
authors.forename: Ragnvi
authors.initials: R
authors.lastname: Hagman
authors.forename: Christoffer
authors.initials: C
authors.lastname: Westin
authors.forename: Odd
authors.initials: O
authors.lastname: Höglund
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D002415:Cats; D002864:Chromogranins / Q000378:metabolism*; D003429:Cross Reactions; D004285:Dogs; D011863:Radioimmunoassay; D013045:Species Specificity
chemical_list: D002864:Chromogranins