title: Generation of monoclonal autoantibodies from Babesia rodhaini-infected mice.
abstract: The presence of anti-erythrocyte autoantibodies in animals infected with various Babesia species is well reported. However, the pathogenesis of autoantibodies in babesiosis is poorly understood. Here, we demonstrated that anti-erythrocyte immunoglobulin (Ig) M and IgG were present in B. rodhaini-infected mice at 6 and 8 days after infection, respectively. Furthermore, we generated monoclonal antibodies against erythrocyte antigen from B. rodhaini-infected mice. Five clones were generated. By Western blotting analysis using whole erythrocyte antigens, one clone reacted with a broad band around 90-150 kDa, and the 2 clones reacted with a band larger than 150 kDa. B. rodhaini-infected mice and/or autoreactive monoclonal antibodies established in this study might be a powerful tool for in vivo pathogenesis studies of autoantibody development in infectious diseases.
authors.affiliation: United Graduate School of Veterinary Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Shih-Pin
authors.initials: SP
authors.lastname: Chiou
authors.forename: Katsuya
authors.initials: K
authors.lastname: Kitoh
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takashima
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000276:immunology* / Q000469:parasitology; D015153:Blotting, Western; D004912:Erythrocytes / Q000276:immunology* / Q000469:parasitology; D005260:Female; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M