title: C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana.
abstract: C3HC4-type RING finger proteins constitute a large family in the plant kingdom and play important roles in various physiological processes of plant life. In this study, a C3HC4-type zinc finger gene was isolated from Nicotiana benthamiana. Sequence analysis indicated that the gene encodes a 24-kDa protein with 191 amino acids containing one typical C3HC4-type zinc finger domain; this gene was named NbZFP1. Transient expression of pGDG-NbZFP1 demonstrated that NbZFP1 was localized to the chloroplast, especially in the chloroplasts of cells surrounding leaf stomata. Virus-induced gene silencing (VIGS) analysis indicated that silencing of NbZFP1 hampered fruit development, although the height of the plants was normal. An overexpression construct was then designed and transferred into Nicotiana benthamiana, and PCR and Southern blot showed that the NbZFP1 gene was successfully integrated into the Nicotiana benthamiana genome. The transgenic lines showed typical compactness, with a short internode length and sturdy stems. This is the first report describing the function of a C3HC4-type RING finger protein in tobacco.
authors.affiliation: The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, China.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Wu
authors.affiliation: The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Liu
authors.affiliation: The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, China.
authors.forename: Xiufen
authors.initials: X
authors.lastname: Yang
authors.affiliation: The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, China.
authors.forename: Dewen
authors.initials: D
authors.lastname: Qiu
mesh_terms: D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000378:metabolism; D003001:Cloning, Molecular; D005638:Fruit / Q000254:growth & development / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000145:classification / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D034622:RNA Interference; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D014026:Nicotiana / Q000254:growth & development* / Q000378:metabolism; D016335:Zinc Fingers
chemical_list: D016376:Oligonucleotides, Antisense; D010940:Plant Proteins; D011994:Recombinant Proteins