title: Substrate binding protein SBP2 of a putative ABC transporter as a novel vaccine antigen of Moraxella catarrhalis.
abstract: Moraxella catarrhalis is a common respiratory tract pathogen that causes otitis media in children and infections in adults with chronic obstructive pulmonary disease. Since the introduction of the pneumococcal conjugate vaccines with/without protein D of nontypeable Haemophilus influenzae, M. catarrhalis has become a high-priority pathogen in otitis media. For the development of antibacterial vaccines and therapies, substrate binding proteins of ATP-binding cassette transporters are important targets. In this study, we identified and characterized a substrate binding protein, SBP2, of M. catarrhalis. Among 30 clinical isolates tested, the sbp2 gene sequence was highly conserved. In 2 different analyses (whole-cell enzyme-linked immunosorbent assay and flow cytometry), polyclonal antibodies raised to recombinant SBP2 demonstrated that SBP2 expresses epitopes on the bacterial surface of the wild type but not the sbp2 mutant. Mice immunized with recombinant SBP2 showed significantly enhanced clearance of M. catarrhalis from the lung compared to that in the control group at both 25-μg and 50-μg doses (P < 0.001). We conclude that SBP2 is a novel, attractive candidate as a vaccine antigen against M. catarrhalis.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University at Buffalo, State University of New York, Buffalo, New York, USA Clinical and Translational Research Center, University at Buffalo, State University of New York, Buffalo, New York, USA.
authors.forename: Taketo
authors.initials: T
authors.lastname: Otsuka
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University at Buffalo, State University of New York, Buffalo, New York, USA Clinical and Translational Research Center, University at Buffalo, State University of New York, Buffalo, New York, USA.
authors.forename: Charmaine
authors.initials: C
authors.lastname: Kirkham
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University at Buffalo, State University of New York, Buffalo, New York, USA Clinical and Translational Research Center, University at Buffalo, State University of New York, Buffalo, New York, USA.
authors.forename: Antoinette
authors.initials: A
authors.lastname: Johnson
authors.affiliation: Clinical and Translational Research Center, University at Buffalo, State University of New York, Buffalo, New York, USA Department of Microbiology and Immunology, University at Buffalo, State University of New York, Buffalo, New York, USA.
authors.forename: Megan M
authors.initials: MM
authors.lastname: Jones
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University at Buffalo, State University of New York, Buffalo, New York, USA Clinical and Translational Research Center, University at Buffalo, State University of New York, Buffalo, New York, USA Department of Microbiology and Immunology, University at Buffalo, State University of New York, Buffalo, New York, USA murphyt@buffalo.edu.
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Murphy
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000276:immunology*; D000328:Adult; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D006801:Humans; D008168:Lung / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D001936:Moraxella catarrhalis / Q000235:genetics / Q000276:immunology*; D045828:Moraxellaceae Infections / Q000276:immunology / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D018528:ATP-Binding Cassette Transporters; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D014614:Vaccines, Synthetic