title: Development of tissue-engineered self-expandable aortic stent grafts (Bio stent grafts) using in-body tissue architecture technology in beagles.
abstract: In this study, we aimed to describe the development of tissue-engineered self-expandable aortic stent grafts (Bio stent graft) using in-body tissue architecture technology in beagles and to determine its mechanical and histological properties. The preparation mold was assembled by insertion of an acryl rod (outer diameter, 8.6 mm; length, 40 mm) into a self-expanding nitinol stent (internal diameter, 9.0 mm; length, 35 mm). The molds (n = 6) were embedded into the subcutaneous pouches of three beagles for 4 weeks. After harvesting and removing each rod, the excessive fragile tissue connected around the molds was trimmed, and thus tubular autologous connective tissues with the stent were obtained for use as Bio stent grafts (outer diameter, approximately 9.3 mm in all molds). The stent strut was completely surrounded by the dense collagenous membrane (thickness, ∼150 µm). The Bio stent graft luminal surface was extremely flat and smooth. The graft wall of the Bio stent graft possessed an elastic modulus that was almost two times higher than that of the native beagle abdominal aorta. This Bio stent graft is expected to exhibit excellent biocompatibility after being implanted in the aorta, which may reduce the risk of type 1 endoleaks or migration.
authors.affiliation: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan; Department of Cardiovascular Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Hidetake
authors.initials: H
authors.lastname: Kawajiri
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mizuno
authors.forename: Takeshi
authors.initials: T
authors.lastname: Moriwaki
authors.forename: Hatsue
authors.initials: H
authors.lastname: Ishibashi-Ueda
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamanami
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kanda
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yaku
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Nakayama
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal; D001807:Blood Vessel Prosthesis; D004285:Dogs; D055119:Elastic Modulus; D008422:Materials Testing; D015607:Stents; D023822:Tissue Engineering / Q000379:methods*
keywords: connective tissue; covered stents; endovascular aortic repair (EVAR); in vivo tissue engineering; stent graft