title: TPM3, a strong prognosis predictor, is involved in malignant progression through MMP family members and EMT-like activators in gliomas.
abstract: Recent studies have shown that many molecular mechanisms, such as the EGFR, AKT, STAT3, and beta-catenin pathways, are involved in glioma. However, the prognosis of the disease remains poor. Explorations of the underlying mechanisms of glioma and identification of effective markers for early diagnosis and accurate prognostication remain important today. In this study, we employed survival analysis to determine that TPM3 overexpression was significantly associated with high-grade gliomas and higher mortality. Using microarray combined with Pearson correlation analysis, we found that TPM3 was positively correlated with the expression of MMP family members and EMT-like activators. Reduction of TPM3 (via TPM3-siRNA) inhibited cellular invasion and migration and decreased MMP-9 and SNAI1 levels in glioma cells. To the best of our knowledge, our work is the first to show that TPM3 plays a critical role in the progression of gliomas and provides novel insights into the key roles of MMP family members and EMT-like activators that mediate TPM3 functional signaling for glioma regulation.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Han
authors.forename: Wenkang
authors.initials: W
authors.lastname: Luan
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.forename: Junxia
authors.initials: J
authors.lastname: Zhang
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongping
authors.initials: Y
authors.lastname: You
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D018450:Disease Progression; D058750:Epithelial-Mesenchymal Transition; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D053208:Kaplan-Meier Estimate; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D011237:Predictive Value of Tests; D011379:Prognosis; D034622:RNA Interference; D000071250:Snail Family Transcription Factors; D046888:Tissue Array Analysis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014335:Tropomyosin / Q000235:genetics / Q000378:metabolism*
chemical_list: C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; C498783:TPM3 protein, human; D014157:Transcription Factors; D014335:Tropomyosin; D020782:Matrix Metalloproteinases; D020780:Matrix Metalloproteinase 9