title: In vitro degradation, hemolysis, and cytocompatibility of PEO/PLLA composite coating on biodegradable AZ31 alloy.
abstract: Magnesium and its alloys have large potential as degradable and absorbable biomaterials because of their mechanical properties and biocompatibility. However, their corrosion resistance is usually inadequate especially in physiological environment, which limits their broad applications in biomedical areas. In this work, plasma electrolytic oxidized/poly(l-lactide) (PEO/PLLA) composite coating was successfully fabricated on biodegradable AZ31 alloy by combing PEO process and sealing with PLLA. The microstructure, elemental composition, and phase composition of the PEO/PLLA composite coating were investigated. The in vitro degradation of the PEO/PLLA composite coating in simulated body fluid (SBF) was also systematically evaluated. The results revealed that the PEO/PLLA composite coating improved the corrosion resistance of AZ31 alloy significantly. The corrosion potential shifted from -1.663V to more positive position -1.317 V and the corrosion current density was reduced with six-order of magnitude. The Mg(2+) ions, hydrogen release, and pH value change of solution caused by degradation were all decreased significantly. Moreover, the PEO process played a critical role in sustaining the integrity of the implant in long-term service. The result of hemolysis test showed that the PEO/PLLA composite coating vested AZ31 alloy a low hemolysis ratio (0.806 ± 0.771)%, which is much lower than the safe value of 5% according to ISO 10993-4. For the cytocompatibility test, compared with bare AZ31 alloy and PEO coating, MC3T3-E1 cells showed much better adhesion and proliferation on the PEO/PLLA composite coating with nearly 4-fold increase of cells after 7-day cultivation, indicating that the PEO/PLLA composite coating has good biocompatibility for biomedical applications.
authors.affiliation: Institute of Materials, Shanghai University, Shanghai, 200072, People's Republic of China.
authors.forename: Zhongling
authors.initials: Z
authors.lastname: Wei
authors.forename: Peng
authors.initials: P
authors.lastname: Tian
authors.forename: Xuanyong
authors.initials: X
authors.lastname: Liu
authors.forename: Bangxin
authors.initials: B
authors.lastname: Zhou
mesh_terms: D000497:Alloys / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects*; D008422:Materials Testing; D051379:Mice; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; C586533:Mg-Al-Zn-Mn-Si-Cu alloy; D011091:Polyesters; C033616:poly(lactide)
keywords: biodegradation; cell adhesion; hemolysis; magnesium; plasma electrolytic oxidation; poly(l-lactide)