title: Chemical constituents of Abies delavayi.
abstract: Systematic phytochemical investigations on Abies delavayi afforded 110 compounds, including 49 terpenoids, 13 lignans, 20 flavonoids, three coumarins, and 25 other chemical constituents. By detailed analysis of one- and two-dimensional NMR spectroscopic and high-resolution mass spectrometric data, 10 previously unreported compounds were identified: they comprised three sesquiterpenoids, two diterpenoids, one triterpenoid, one monoterpenoid, one flavonoid, and two phenols. These 10 compounds and some previously known ones were subjected to two cytotoxic bioassays against three human tumor cell lines and NO production inhibition on RAW264.7 macrophages, respectively. (25R)-24,25-Dihydroabieslactone had the strongest cytotoxic activity against Colo-205 cells with an IC50 value of 19.0±3.7μg/mL. (+)-T-cadinol, 8,11,13-abietatrien-15-ol-18-yl acetate, 18-acetoxy-13-epi-manool, imperatorin, bergapten, and 5,7-O-dimethyl poriol exhibited weak inhibitory activity against LPS-induced NO production in RAW264.7 macrophages with IC50 values of approximately 50μg/mL.
authors.affiliation: Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, China; Key Laboratory of Marine Bio-Resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, China; Luxembourg Public Research Centre for Health (CRP-SANTE), 84 Val Fleuri, L-1526 Luxembourg, Luxembourg.
authors.forename: Xian-Wen
authors.initials: XW
authors.lastname: Yang
authors.affiliation: Key Laboratory of Marine Bio-Resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, China.
authors.forename: Su-Mei
authors.initials: SM
authors.lastname: Li
authors.affiliation: Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, China.
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Li
authors.affiliation: Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, China.
authors.forename: Yun-Heng
authors.initials: YH
authors.lastname: Shen
authors.affiliation: Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, China.
authors.forename: Jun-Mian
authors.initials: JM
authors.lastname: Tian
authors.affiliation: Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, China.
authors.forename: Hua-Wu
authors.initials: HW
authors.lastname: Zeng
authors.affiliation: Luxembourg Public Research Centre for Health (CRP-SANTE), 84 Val Fleuri, L-1526 Luxembourg, Luxembourg.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Luxembourg Public Research Centre for Health (CRP-SANTE), 84 Val Fleuri, L-1526 Luxembourg, Luxembourg.
authors.forename: Andre
authors.initials: A
authors.lastname: Steinmetz
authors.affiliation: Key Laboratory of Marine Bio-Resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, China. Electronic address: wdzhangy@hotmail.com.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
mesh_terms: D028202:Abies / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D017705:Lignans / Q000737:chemistry; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification; D035261:Plant Components, Aerial / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D017705:Lignans; D008070:Lipopolysaccharides; D039821:Monoterpenes; D010636:Phenols; D012717:Sesquiterpenes; D009569:Nitric Oxide; C072576:T-cadinol; D004317:Doxorubicin; C482828:manool
keywords: Abies delavayi; Cytotoxicity; Monoterpenes; Nitric oxide (NO); Phenols; Pinaceae; Sesquiterpenes; Triterpenoids