title: Assessing acceptor substrate promiscuity of YjiC-mediated glycosylation toward flavonoids.
abstract: The acceptor substrate promiscuity of YjiC, a UDP-glycosyltransferase from Bacillus licheniformis, was explored with seven different classes (flavonols, flavanols, flavones, flavanones, chalcone, stilbene, and isoflavonoids) of 23 flavonoid acceptors. For most of the polyphenols used in the reactions, the enzymatic bioconversion was significantly higher with the production of multiple glucosylated derivatives. This study highlights the highly flexible non-regiospecific glycosylation ability of YjiC toward polyphenolic compounds. The catalytic potential of YjiC could be useful to generate a library of natural product glucosides.
authors.affiliation: Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, 100 Kalsan-ri, Tangjeonmyun, Asan-si, Chungnam 336-708, Republic of Korea.
authors.forename: Ramesh Prasad
authors.initials: RP
authors.lastname: Pandey
authors.affiliation: Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, 100 Kalsan-ri, Tangjeonmyun, Asan-si, Chungnam 336-708, Republic of Korea.
authors.forename: Rit Bahadur
authors.initials: RB
authors.lastname: Gurung
authors.affiliation: Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, 100 Kalsan-ri, Tangjeonmyun, Asan-si, Chungnam 336-708, Republic of Korea.
authors.forename: Prakash
authors.initials: P
authors.lastname: Parajuli
authors.affiliation: Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, 100 Kalsan-ri, Tangjeonmyun, Asan-si, Chungnam 336-708, Republic of Korea.
authors.forename: Niranjan
authors.initials: N
authors.lastname: Koirala
authors.affiliation: Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, 100 Kalsan-ri, Tangjeonmyun, Asan-si, Chungnam 336-708, Republic of Korea.
authors.forename: Le Thi
authors.initials: le T
authors.lastname: Tuoi
authors.affiliation: Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, 100 Kalsan-ri, Tangjeonmyun, Asan-si, Chungnam 336-708, Republic of Korea. Electronic address: sohng@sunmoon.ac.kr.
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism*; D005960:Glucosides / Q000096:biosynthesis* / Q000737:chemistry; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016415:Sequence Alignment; D013379:Substrate Specificity
chemical_list: D005419:Flavonoids; D005960:Glucosides; D016695:Glycosyltransferases
keywords: Flavonoids; Glycosyltransferase; Natural product glucosides; Non-regiospecific glycosylation