title: Preparation and enzymatic degradation of porous crosslinked polylactides of biomass origin.
abstract: To understand the enzymatic degradation behavior of crosslinked polylactide (PLA), the preparation and enzymatic degradation of both thermoplastic (linear) and crosslinked PLAs that have pore structures with different dimensions were carried out. The porous structures of the linear PLA samples were of micro and nanoporous nature, and prepared by batch foaming with supercritical CO2 and compared with the porous structures of crosslinked PLA (Lait-X) created by the salt leaching method. The surface and cross-sectional morphologies of the porous structures were investigated by using scanning electron microscopy. The morphological analysis of porous Lait-X showed a rapid loss of physical features within 120 h of exposure to proteinase-K enzymatic degradation at 37 °C. Due to the higher affinity for water, enhanced enzymatic activity as compared to the linear PLA porous structures in the micro and nanoporous range was observed.
authors.affiliation: Advanced Polymeric Nanostructured Materials Engineering, Graduate School of Engineering, Toyota Technological Institute, 2-12-1 Hisakata, Tempaku, Nagoya 468-8511, Japan. k.i.d-41-fw@ezweb.ne.jp.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kido
authors.affiliation: Advanced Polymeric Nanostructured Materials Engineering, Graduate School of Engineering, Toyota Technological Institute, 2-12-1 Hisakata, Tempaku, Nagoya 468-8511, Japan. perfect_blue0225@hotmail.com.
authors.forename: Reika
authors.initials: R
authors.lastname: Sakai
authors.affiliation: Advanced Polymeric Nanostructured Materials Engineering, Graduate School of Engineering, Toyota Technological Institute, 2-12-1 Hisakata, Tempaku, Nagoya 468-8511, Japan. jonsbaiju@gmail.com.
authors.forename: Baiju
authors.initials: B
authors.lastname: John
authors.affiliation: Advanced Polymeric Nanostructured Materials Engineering, Graduate School of Engineering, Toyota Technological Institute, 2-12-1 Hisakata, Tempaku, Nagoya 468-8511, Japan. okamoto@toyota-ti.ac.jp.
authors.forename: Masami
authors.initials: M
authors.lastname: Okamoto
authors.affiliation: Department of Biotechnology and Chemical Technology, Aalto University, P.O. BOX 16100, Aalto FI-00076, Finland. jukka.seppala@aalto.fi.
authors.forename: Jukka V
authors.initials: JV
authors.lastname: Seppälä
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D019286:Endopeptidase K / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C033616:poly(lactide); D019286:Endopeptidase K