title: Different miRNA expression profiles between human breast cancer tumors and serum.
abstract: A bunch of microRNAs (miRNAs) have been demonstrated to be aberrantly expressed in cancer tumor tissue and serum. The miRNA signatures identified from the serum samples could serve as potential noninvasive diagnostic markers for breast cancer. The role of the miRNAs in cancerigenesis is unclear. In this study, we generated the expression profiles of miRNAs from the paired breast cancer tumors, normal, tissue, and serum samples from eight patients using small RNA-sequencing. Serum samples from eight healthy individuals were used as normal controls. We identified total 174 significantly differentially expressed miRNAs between tumors and the normal tissues, and 109 miRNAs between serum from patients and serum from healthy individuals. There are only 10 common miRNAs. This suggests that only a small portion of tumor miRNAs are released into serum selectively. Interestingly, the expression change pattern of 28 miRNAs is opposite between breast cancer tumors and serum. Functional analysis shows that the differentially expressed miRNAs and their target genes form a complex interaction network affecting many biological processes and involving in many types of cancer such as prostate cancer, basal cell carcinoma, acute myeloid leukemia, and more.
authors.affiliation: Clinical laboratory, Taizhou Central Hospital, Taizhou, Zhejiang, China Zhejiang, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Oncology, Taizhou Central Hospital, Taizhou Zhejiang, China.
authors.forename: Zhibao
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Tumor Hospital of Zhejiang Province, Hangzhou Zhejiang, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: JoinGenome Bioinformatics Company, Hangzhou Zhejiang, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Sun
authors.affiliation: Clinical laboratory, Taizhou Central Hospital, Taizhou, Zhejiang, China Zhejiang, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.affiliation: JoinGenome Bioinformatics Company, Hangzhou Zhejiang, China.
authors.forename: Xianying
authors.initials: X
authors.lastname: Cheng
authors.affiliation: School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou Zhejiang, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Fu
authors.affiliation: Clinical laboratory, Taizhou Central Hospital, Taizhou, Zhejiang, China Zhejiang, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Clinical laboratory, Taizhou Central Hospital, Taizhou, Zhejiang, China Zhejiang, China.
authors.forename: Zuojun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Clinical laboratory, Taizhou Central Hospital, Taizhou, Zhejiang, China Zhejiang, China ; School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou Zhejiang, China.
authors.forename: Zhaoyun
authors.initials: Z
authors.lastname: Li
keywords: biomarker; breast cancer; miRNA; serum; tumor