title: Tandem mass spectral libraries of peptides in digests of individual proteins: Human Serum Albumin (HSA).
abstract: This work presents a method for creating a mass spectral library containing tandem spectra of identifiable peptide ions in the tryptic digestion of a single protein. Human serum albumin (HSA(1)) was selected for this purpose owing to its ubiquity, high level of characterization and availability of digest data. The underlying experimental data consisted of ∼3000 one-dimensional LC-ESI-MS/MS runs with ion-trap fragmentation. In order to generate a wide range of peptides, studies covered a broad set of instrument and digestion conditions using multiple sources of HSA and trypsin. Computer methods were developed to enable the reliable identification and reference spectrum extraction of all peptide ions identifiable by current sequence search methods. This process made use of both MS2 (tandem) spectra and MS1 (electrospray) data. Identified spectra were generated for 2918 different peptide ions, using a variety of manually-validated filters to ensure spectrum quality and identification reliability. The resulting library was composed of 10% conventional tryptic and 29% semitryptic peptide ions, along with 42% tryptic peptide ions with known or unknown modifications, which included both analytical artifacts and post-translational modifications (PTMs) present in the original HSA. The remaining 19% contained unexpected missed-cleavages or were under/over alkylated. The methods described can be extended to create equivalent spectral libraries for any target protein. Such libraries have a number of applications in addition to their known advantages of speed and sensitivity, including the ready re-identification of known PTMs, rejection of artifact spectra and a means of assessing sample and digestion quality.
authors.affiliation: From the ‡Biomolecular Measurement Division, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8362, Gaithersburg, Maryland 20899, United States.
authors.forename: Qian
authors.initials: Q
authors.lastname: Dong
authors.affiliation: From the ‡Biomolecular Measurement Division, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8362, Gaithersburg, Maryland 20899, United States.
authors.forename: Xinjian
authors.initials: X
authors.lastname: Yan
authors.affiliation: From the ‡Biomolecular Measurement Division, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8362, Gaithersburg, Maryland 20899, United States.
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Kilpatrick
authors.affiliation: From the ‡Biomolecular Measurement Division, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8362, Gaithersburg, Maryland 20899, United States.
authors.forename: Yuxue
authors.initials: Y
authors.lastname: Liang
authors.affiliation: From the ‡Biomolecular Measurement Division, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8362, Gaithersburg, Maryland 20899, United States.
authors.forename: Yuri A
authors.initials: YA
authors.lastname: Mirokhin
authors.affiliation: From the ‡Biomolecular Measurement Division, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8362, Gaithersburg, Maryland 20899, United States.
authors.forename: Jeri S
authors.initials: JS
authors.lastname: Roth
authors.affiliation: From the ‡Biomolecular Measurement Division, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8362, Gaithersburg, Maryland 20899, United States.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Rudnick
authors.affiliation: From the ‡Biomolecular Measurement Division, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8362, Gaithersburg, Maryland 20899, United States.
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Stein
mesh_terms: D002853:Chromatography, Liquid; D006801:Humans; D019151:Peptide Library; D059748:Proteolysis; D012709:Serum Albumin / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000737:chemistry
chemical_list: D019151:Peptide Library; D012709:Serum Albumin; D014357:Trypsin