title: Live-cell fluorescence imaging for phenotypic analysis of mitosis.
abstract: Live-cell fluorescence microscopy is a powerful tool for characterizing aberrant mitotic phenotypes resulting from exposure to chemical inhibitors or after depletion of protein targets by RNA interference or other methods. Live imaging of cultured cells during mitotic progression presents challenges in maintaining optimal health of cells while achieving the temporal and spatial resolution to accomplish the goals of the study. We describe herein strategies to monitor and analyze mammalian cell mitosis with standard, inverted, fluorescence microscopy systems that are widely available.
authors.affiliation: Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, MS 48, Oklahoma City, OK, 73104, USA.
authors.forename: Sushama
authors.initials: S
authors.lastname: Sivakumar
authors.forename: John R
authors.initials: JR
authors.lastname: Daum
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Gorbsky
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival; D049452:Green Fluorescent Proteins / Q000032:analysis; D006367:HeLa Cells; D006657:Histones / Q000032:analysis; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D008938:Mitosis; D061848:Optical Imaging / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000032:analysis
chemical_list: D006657:Histones; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins