title: Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation.
abstract: According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection.
authors.affiliation: The London School of Hygiene & Tropical Medicine, Keppel St, London WC1E 7HT, United Kingdom ; School of Life Sciences, Faculty of Science, Engineering and Computing, Kingston University, Kingston upon Thames, Penrhyn Road, KT1 2EE, United Kingdom.
authors.forename: A V
authors.initials: AV
authors.lastname: Karlyshev
authors.affiliation: The London School of Hygiene & Tropical Medicine, Keppel St, London WC1E 7HT, United Kingdom.
authors.forename: G
authors.initials: G
authors.lastname: Thacker
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Loughborough, Sutton Bonnington LE12 5RD, United Kingdom.
authors.forename: M A
authors.initials: MA
authors.lastname: Jones
authors.affiliation: School of Biosciences, University of Westminster, 115 New Cavendish Street, London W1B 2UW, United Kingdom.
authors.forename: M O
authors.initials: MO
authors.lastname: Clements
authors.affiliation: The London School of Hygiene & Tropical Medicine, Keppel St, London WC1E 7HT, United Kingdom.
authors.forename: B W
authors.initials: BW
authors.lastname: Wren
keywords: Campylobacter; Colonisation; Peptidases; Protease inhibitors; Proteases; Proteolysis