title: A new magnetic nanocapsule containing 5-fluorouracil: in vivo drug release, anti-tumor, and pro-apoptotic effects on CT26 cells allograft model.
abstract: The purpose of this study was to create an optimized method for preparation of 5-fluorouracil-loaded magnetic poly lactic-co-glycolic acid nanocapsules and to investigate its potential as multifunctional carriers to deliver therapeutic agents for tumor-targeted therapies. The in vitro release of the newly synthesized 5-fluorouracil-loaded poly lactic-co-glycolic acid magnetic nanocapsules was investigated in phosphate-buffered saline medium using the dialysis method. In vivo release studies of the magnetic nanocapsules were performed in rabbits. Finally, the targeting properties, anti-tumor, and pro-apoptotic effects of this new magnetic nanocapsule on CT26 cells allograft model were studied. The effective diameter of nanocapsules was 67.2 nm. In vivo release investigations showed that 5-fluorouracil has a sustained release profile, prolonged lifetime in the rabbit plasma, and increased tissue appetency when loaded into the magnetic nanocapsule. Magnetic resonance imaging confirmed that the magnetic nanocapsules were successfully targeted to the tumor. Additionally, the anti-tumor studies revealed that the targeted therapy with magnetic nanocapsules containing 5-fluorouracil effectively inhibits the growth of tumors compared with 5-fluorouracil alone (P < 0.01). The present study demonstrates that this new magnetic nanocapsule can be considered a new nanotechnology-based cancer chemotherapy agent in vivo.
authors.affiliation: Medical Physics Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Shakeri-Zadeh
authors.affiliation: Medical Physics Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad-Bagher
authors.initials: MB
authors.lastname: Shiran
authors.affiliation: Polymer Chemistry Department, School of Sciences, University of Tehran, Tehran, Iran.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Khoee
authors.affiliation: Razi Drug Research Centre, Iran University of Medical Sciences, Tehran, Iran Pharmacology Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Ali Mohammad
authors.initials: AM
authors.lastname: Sharifi
authors.affiliation: Razi Drug Research Centre, Iran University of Medical Sciences, Tehran, Iran Pharmacology Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Habib
authors.initials: H
authors.lastname: Ghaznavi
authors.affiliation: Medical Physics Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Razi Drug Research Centre, Iran University of Medical Sciences, Tehran, Iran khoei.s@iums.ac.ir skhoei@gmail.com.
authors.forename: Samideh
authors.initials: S
authors.lastname: Khoei
mesh_terms: D064591:Allografts; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D005472:Fluorouracil / Q000008:administration & dosage* / Q000493:pharmacokinetics; D019344:Lactic Acid / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D053769:Nanocapsules / Q000737:chemistry; D036103:Nanotechnology; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits
chemical_list: D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005472:Fluorouracil
keywords: 5-fluorouracil; Colon cancer; PLGA; magnetic nanocapsule; nanotechnology