title: The melanogenesis-inhibitory effect and the percutaneous formulation of ginsenoside Rb1.
abstract: Ginsenoside Rb1 (Rb1) is the most predominant ginsenoside isolated from the roots of ginseng (Panax ginseng C. A. Meyer). This compound is active in various human biological pathways that are involved in human collagen synthesis and inhibition of cell apoptosis. In this study, the skin-whitening effects of Rb1 were investigated in B16 melanoma cells. Our results showed that Rb1 inhibited melanogenesis in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16 cells in a dose-dependent manner, which collectively indicated that Rb1 may have skin-whitening effects and may be formulated into skin-whitening products for skin care. Accordingly, a ginsenoside collagen transdermal patch was developed as a vehicle to topically deliver Rb1 into pig skin. The percutaneous permeation, retention within skin, and release in vitro of Rb1 from seven transdermal patch formulas were studied. It was determined that the best formula for ginsenoside collagen transdermal patch is made of protein collagen hydrolysate powder (PCHP) 2.0% (w/w), methyl cellulose (MC) 0.5% (w/w), polyethyleneglycol 6000 (PEG6000) 0.5% (w/w), ginsenoside 0.036% (w/w), azone 0.4% (v/w), menthol 0.20% (w/w), and water.
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, 7 Baptist University Road, Kowlong Tong, Kowloon, Hong Kong, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Lu
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Yu
authors.forename: Ricky N S
authors.initials: RN
authors.lastname: Wong
authors.forename: Zhao-Xiang
authors.initials: ZX
authors.lastname: Bian
authors.forename: Hoi-Hin
authors.initials: HH
authors.lastname: Kwok
authors.forename: Patrick Ying-Kit
authors.initials: PY
authors.lastname: Yue
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Zhou
authors.forename: HuBiao
authors.initials: H
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D003094:Collagen; D003692:Delayed-Action Preparations; D036145:Ginsenosides / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D017495:Hyperpigmentation / Q000188:drug therapy*; D066298:In Vitro Techniques; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010539:Permeability; D013552:Swine; D057968:Transdermal Patch
chemical_list: D003692:Delayed-Action Preparations; D036145:Ginsenosides; D008543:Melanins; C442759:ginsenoside Rb1; D003094:Collagen; D014442:Monophenol Monooxygenase