title: Water-soluble triarylboron compound for ATP imaging in vivo using analyte-induced finite aggregation.
abstract: Adenosine 5'-triphosphate (ATP) is a multifunctional molecule that participates in many important biological processes. Currently, fluorescence indicators for ATP with high performance are in demand. Reported herein is a novel water-soluble triarylboron compound which displays an apparent ATP-dependent fluorescence enhancement when dispersed in water. It can selectively recognize ATP from other bioactive substances in vitro and in vivo. The ATP-induced finite aggregation endows the indicator with appreciable photostability and superior tolerance to environmental electrolytes. This indicator has been successfully applied to the ATP imaging in NIH/3T3 fibroblast cells. The difference in the ATP levels within the membrane and cytosol is clearly visible.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (China).
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.forename: Xudong
authors.initials: X
authors.lastname: Guo
authors.forename: Lixia
authors.initials: L
authors.lastname: Cao
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Xun
authors.forename: Shuangqing
authors.initials: S
authors.lastname: Wang
authors.forename: Shayu
authors.initials: S
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D001896:Boron Compounds / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects*
chemical_list: D001896:Boron Compounds; D000255:Adenosine Triphosphate
keywords: aggregation; cell recognition; fluorescence; imaging agents; molecular recognition