title: MiR-186 targets ROCK1 to suppress the growth and metastasis of NSCLC cells.
abstract: MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in human cancers. Increasing evidence shows that deregulation of miRNAs contributes to the development and progression of human non-small cell lung cancer (NSCLC). Here, we identified miR-186 as a tumor suppressor in NSCLC, which was decreased in NSCLC. Overexpression of miR-186 significantly inhibited proliferation, migration, and invasion of NSCLC cells. In addition, Rho-associated protein kinase 1 (ROCK1) was identified as a target of miR-186 in NSCLC cells. Restoration of ROCK1 remarkably reversed the tumor-suppressive effects of miR-186 on cell proliferation, migration, and invasion in NSCLC cells. Furthermore, ROCK1 was inversely correlated with miR-186 expression in NSCLC. Collectively, our data indicate that miR-186 functions as tumor suppressor in NSCLC by targeting ROCK1.
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, 450000, Zhengzhou, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Cui
authors.forename: Mingwei
authors.initials: M
authors.lastname: Cui
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Li
authors.forename: Youguang
authors.initials: Y
authors.lastname: Liang
authors.forename: Weihao
authors.initials: W
authors.lastname: Li
authors.forename: Haizhou
authors.initials: H
authors.lastname: Guo
authors.forename: Song
authors.initials: S
authors.lastname: Zhao
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009154:Mutation; D009362:Neoplasm Metastasis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D054460:rho-Associated Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C533096:MIRN186 microRNA, human; D035683:MicroRNAs; D008156:Luciferases; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases