title: Angiostrongylus vasorum in wolves in Italy.
abstract: In the past decade, the parasitic nematode Angiostrongylus vasorum has attracted attention for its emergence in previously free areas and for the rise in clinical cases in domestic dogs. Italy is regarded as one of the countries where this potentially life-threatening parasite is spreading, especially due to bridging infections between wildlife and domestic dogs. The present article describes the presence of A. vasorum in wolves from Italy. Nematodes were observed in histological sections of three wolves found dead in Rome province, central Italy. Morphological and molecular identification of the nematodes, by polymerase chain reaction of rDNA ITS-2 and sequencing, confirmed the nematodes to be A. vasorum, with 99% genetic homology with A. vasorum from sympatric dogs. This is the second report of this species in wolves and the first in this host in Italy, and coincides with increasing records of A. vasorum in dogs and foxes in Italy. Implications for the epidemiology of this emerging parasite and for wildlife health are concisely discussed.
authors.affiliation: Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Eleni
authors.affiliation: Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: De Liberato
authors.affiliation: School of Biological Sciences, University of Bristol, UK.
authors.forename: Dena
authors.initials: D
authors.lastname: Azam
authors.affiliation: School of Biological Sciences, University of Bristol, UK.
authors.forename: Eric R
authors.initials: ER
authors.lastname: Morgan
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Italy.
authors.forename: Donato
authors.initials: D
authors.lastname: Traversa
keywords: Angiostrongylosis; Canid; Epidemiology; Lungworm; One health; Pathology; Wildlife disease; Wolf