title: Sequential in vivo imaging of osteogenic stem/progenitor cells during fracture repair.
abstract: Bone turns over continuously and is highly regenerative following injury. Osteogenic stem/progenitor cells have long been hypothesized to exist, but in vivo demonstration of such cells has only recently been attained. Here, in vivo imaging techniques to investigate the role of endogenous osteogenic stem/progenitor cells (OSPCs) and their progeny in bone repair are provided. Using osteo-lineage cell tracing models and intravital imaging of induced microfractures in calvarial bone, OSPCs can be directly observed during the first few days after injury, in which critical events in the early repair process occur. Injury sites can be sequentially imaged revealing that OSPCs relocate to the injury, increase in number and differentiate into bone forming osteoblasts. These methods offer a means of investigating the role of stem cell-intrinsic and extrinsic molecular regulators for bone regeneration and repair.
authors.affiliation: Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Stem Cell Institute; dongsu.park@bcm.edu.
authors.forename: Dongsu
authors.initials: D
authors.lastname: Park
authors.affiliation: Wellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School.
authors.forename: Joel A
authors.initials: JA
authors.lastname: Spencer
authors.affiliation: Wellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School.
authors.forename: Charles P
authors.initials: CP
authors.lastname: Lin
authors.affiliation: Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Stem Cell Institute; david_scadden@harvard.edu.
authors.forename: David T
authors.initials: DT
authors.lastname: Scadden
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D017102:Fracture Healing / Q000502:physiology*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D064547:Myxovirus Resistance Proteins / Q000235:genetics / Q000378:metabolism; D010011:Osteocytes / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: C579070:Mx1 protein, mouse; D064547:Myxovirus Resistance Proteins