title: A novel three-dimensional biosensor based on aluminum oxide: application for early-stage detection of human interleukin-10.
abstract: Immunosensors based on electrolyte-oxide-semiconductors (EOS) have been extensively researched over the last few decades. By electrochemical impedance spectroscopy (EIS) the specific molecular biorecognition of the antibody-antigen (Ab-Ag) can be detected providing an alternative quantitative system to immunoassay techniques. The electrochemical variations from a fabricated immunosensor can provide quantitative values for the analyte of interest at reduced costs and analysis time. In this context, a novel EOS substrate based on aluminum oxide (Al2O3) grown by atomic layer deposition on silicon was applied. The interaction between recombinant human (rh) interleukin-10 (IL-10) with the corresponding monoclonal antibody (mAb) for early cytokine detection of an anti-inflammatory response due to left ventricular assisted device implantation was studied. For this purpose, a 3D biosensor was composed of multi-walled carbon nanotubes with carboxylic acid functionalities (multi-walled carbon nanotubes-COOH) to increase the surface area for the range of human IL-10 detection. These were activated with N-hydroxysuccinimide and N-(3-dimethylaminopropyl)-N'-ethyl-carbodiimide hydrochloride for the immobilization of the anti-human IL-10 mAb. First, the interaction between the Ab and Ag was observed by fluorescence patterning to ensure that the biorecognition event was achievable. Then, EIS is explained for the quantification of commercial human IL-10 on this capacitance-based EOS macroimmuno-FET sensor.
authors.affiliation: Institut de Sciences Analytiques (ISA) - UMR 5280, Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne, France, michael.lee@oulook.es.
authors.forename: Michael
authors.initials: M
authors.lastname: Lee
authors.forename: Abdoullatif
authors.initials: A
authors.lastname: Baraket
authors.forename: Nadia
authors.initials: N
authors.lastname: Zine
authors.forename: Miguel
authors.initials: M
authors.lastname: Zabala
authors.forename: Francesca
authors.initials: F
authors.lastname: Campabadal
authors.forename: Raffaele
authors.initials: R
authors.lastname: Caruso
authors.forename: Maria Giovanna
authors.initials: MG
authors.lastname: Trivella
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic-Renault
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Errachid
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D055910:Antibodies, Immobilized / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D058266:Dielectric Spectroscopy; D004566:Electrodes; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D016753:Interleukin-10 / Q000032:analysis*; D043942:Nanotubes / Q000737:chemistry; D012666:Semiconductors; D012825:Silicon / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D013388:Succinimides; D016753:Interleukin-10; D002244:Carbon; D000537:Aluminum Oxide; C001426:N-hydroxysuccinimide; D012825:Silicon