title: Utilization of coffee by-products obtained from semi-washed process for production of value-added compounds.
abstract: The semi-dry processing of coffee generates significant amounts of coffee pulp and wastewater. This study evaluated the production of bioethanol and volatile compounds of eight yeast strains cultivated in a mixture of these residues. Hanseniaspora uvarum UFLA CAF76 showed the best fermentation performance; hence it was selected to evaluate different culture medium compositions and inoculum size. The best results were obtained with 12% w/v of coffee pulp, 1 g/L of yeast extract and 0.3 g/L of inoculum. Using these conditions, fermentation in 1 L of medium was carried out, achieving higher ethanol yield, productivity and efficiency with values of 0.48 g/g, 0.55 g/L h and 94.11% respectively. Twenty-one volatile compounds corresponding to higher alcohols, acetates, terpenes, aldehydes and volatile acids were identified by GC-FID. Such results indicate that coffee residues show an excellent potential as substrates for production of value-added compounds. H. uvarum demonstrated high fermentative capacity using these residues.
authors.affiliation: Department of Biology, Federal University of Lavras (UFLA), CP 3037 - Campus Universitário, CEP 37.200-000 Lavras, MG, Brazil.
authors.forename: Verónica Alejandra
authors.initials: VA
authors.lastname: Bonilla-Hermosa
authors.affiliation: Department of Biology, Federal University of Lavras (UFLA), CP 3037 - Campus Universitário, CEP 37.200-000 Lavras, MG, Brazil. Electronic address: whasleyduarte@dbi.ufla.br.
authors.forename: Whasley Ferreira
authors.initials: WF
authors.lastname: Duarte
authors.affiliation: Department of Biology, Federal University of Lavras (UFLA), CP 3037 - Campus Universitário, CEP 37.200-000 Lavras, MG, Brazil.
authors.forename: Rosane Freitas
authors.initials: RF
authors.lastname: Schwan
mesh_terms: D056804:Biofuels; D019149:Bioreactors; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003069:Coffee / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D025341:Principal Component Analysis; D013045:Species Specificity; D055549:Volatile Organic Compounds / Q000378:metabolism*; D014866:Waste Products; D015003:Yeasts / Q000378:metabolism*
chemical_list: D056804:Biofuels; D003069:Coffee; D055549:Volatile Organic Compounds; D014866:Waste Products; D000431:Ethanol
keywords: Bioethanol; Coffee pulp; Coffee wastewater; Hanseniaspora uvarum; Volatile compounds