title: Microbial bioproducts from cheese whey through fermentation with wastewater sludge Clostridium isolates.
abstract: We demonstrated the production of hydrogen, ethanol, and a variety of acids by several Clostridium species using cheese whey as substrate. These species were isolated from the anaerobic sediments of a municipal wastewater stabilization pond. Eight isolates were obtained and all were classified taxonomically as Clostridium spp. based on 16S rRNA sequencing. Sludge isolates showed maximum bioproduct production yields and productivities after approximately 24 h of batch cultivation with 6% (m/v) cheese whey. Fermentation byproducts measured included hydrogen, ethanol, acetic acid, butyric acid, and lactic acid. The maximum yields of bioproducts were 0.59 mol H(2)/mol lactose, 0.071 g ethanol/g, 0.204 g acetic acid/g, 0.218 g butyric acid/g, and 0.144 g lactic acid/g. The production of these high value biofuels and biofuel intermediates from cheese whey could have significant implications for conversion of waste to high value bioproducts to enhance domestic energy economies.
authors.affiliation: Utah State University, Department of Biological Engineering, 4105 Old Main Hill, Logan, UT 84322-4105, USA.
authors.forename: Joshua T
authors.initials: JT
authors.lastname: Ellis
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Sims
authors.forename: Charles D
authors.initials: CD
authors.lastname: Miller
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D056804:Biofuels; D001688:Biological Products / Q000378:metabolism*; D020148:Butyric Acid / Q000378:metabolism; D002611:Cheese / Q000382:microbiology*; D003013:Clostridium / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D012722:Sewage / Q000382:microbiology; D062065:Wastewater / Q000382:microbiology*
chemical_list: D019342:Acetic Acid; D056804:Biofuels; D001688:Biological Products; D020148:Butyric Acid; D000431:Ethanol; D006859:Hydrogen; D019344:Lactic Acid; D007785:Lactose; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater
keywords: Clostridium; acides; acids; cheese whey; hydrogen; hydrogène; lactosérum de fromage; solvants; solvents