title: Identification of virulence factors genes in Escherichia coli isolates from women with urinary tract infection in Mexico.
abstract: E coli isolates (108) from Mexican women, clinically diagnosed with urinary tract infection, were screened to identify virulence genes, phylogenetic groups, and antibiotic resistance. Isolates were identified by MicroScan4 system; additionally, the minimum inhibitory concentration (MIC) was assessed. The phylogenetic groups and 16 virulence genes encoding adhesins, toxins, siderophores, lipopolysaccharide (LPS), and invasins were identified by PCR. Phylogenetic groups distribution was as follows: B1 9.3%, A 30.6%, B2 55.6%, and D 4.6%. Virulence genes prevalence was ecp 98.1%, fimH 86.1%, traT 77.8%, sfa/focDE 74.1%, papC 62%, iutA 48.1%, fyuA 44.4%, focG 2.8%, sfaS 1.9%, hlyA 7.4%, cnf-1 6.5%, cdt-B 0.9%, cvaC 2.8%, ibeA 2.8%, and rfc 0.9%. Regarding antimicrobial resistance it was above 50% to ampicillin/sulbactam, ampicillin, piperacillin, trimethoprim/sulfamethoxazole, ciprofloxacin, and levofloxacin. Uropathogenic E. coli clustered mainly in the pathogenic phylogenetic group B2. The isolates showed a high presence of siderophores and adhesion genes and a low presence of genes encoding toxins. The high frequency of papC gene suggests that these isolates have the ability to colonize the kidneys. High resistance to drugs considered as first choice treatment such as trimethoprim/sulfamethoxazole and fluoroquinolones was consistently observed.
authors.affiliation: Department of Ecology of Pathogen Agents, Hospital General "Dr. Manuel Gea González", 14080 Tlalpan, DF, Mexico.
authors.forename: Daniela A
authors.initials: DA
authors.lastname: López-Banda
authors.affiliation: Department of Molecular Biology and Histocompatibility, Hospital General "Dr. Manuel Gea González", 14080 Tlalpan, DF, Mexico.
authors.forename: Erika M
authors.initials: EM
authors.lastname: Carrillo-Casas
authors.affiliation: Department of Molecular Biology and Histocompatibility, Hospital General "Dr. Manuel Gea González", 14080 Tlalpan, DF, Mexico.
authors.forename: Margarita
authors.identifier: 0000-0002-8162-7336
authors.initials: M
authors.lastname: Leyva-Leyva
authors.affiliation: Institute of Cell Physiology, Universidad Nacional Autónoma de México, 04510 Coyoacán, DF, Mexico.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Orozco-Hoyuela
authors.affiliation: Department of Public Health, Universidad Nacional Autónoma de México, 04510 Coyoacán, DF, Mexico.
authors.forename: Ángel H
authors.initials: ÁH
authors.lastname: Manjarrez-Hernández
authors.affiliation: Department of Molecular Biology and Histocompatibility, Hospital General "Dr. Manuel Gea González", 14080 Tlalpan, DF, Mexico.
authors.forename: Sara
authors.initials: S
authors.lastname: Arroyo-Escalante
authors.affiliation: Clinical Laboratory, Hospital General "Dr. Manuel Gea González", 14080 Tlalpan, DF, Mexico.
authors.forename: David
authors.initials: D
authors.lastname: Moncada-Barrón
authors.affiliation: Clinical Laboratory, Hospital General "Dr. Manuel Gea González", 14080 Tlalpan, DF, Mexico.
authors.forename: Silvia
authors.initials: S
authors.lastname: Villanueva-Recillas
authors.affiliation: Department of Infectology, Hospital Infantil de México "Federico Gómez", 06720 Cuauhtémoc, DF, Mexico.
authors.forename: Juan
authors.initials: J
authors.lastname: Xicohtencatl-Cortes
authors.affiliation: Department of Ecology of Pathogen Agents, Hospital General "Dr. Manuel Gea González", 14080 Tlalpan, DF, Mexico.
authors.forename: Rigoberto
authors.initials: R
authors.lastname: Hernández-Castro
mesh_terms: D000328:Adult; D004352:Drug Resistance, Microbial / Q000187:drug effects / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008800:Mexico; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014552:Urinary Tract Infections / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D037521:Virulence Factors