title: Inhibition of FASN reduces the synthesis of medium-chain fatty acids in goat mammary gland.
abstract: Fatty acid synthase (FASN) is known as a crucial enzyme of cellular de novo fatty acid synthesis in mammary gland which has been proved as the main source of short and medium-chain fatty acids of milk. However, the regulatory role of FASN in goat-specific milk fatty acids composition remains unclear. We cloned and analyzed the full-length of FASN gene from the mammary gland of Capra hircus (Xinong Saanen dairy goat) (DQ 915966). Comparative gene expression analysis suggested that FASN is predominantly expressed in fat, small intestine and mammary gland tissues, and expresses higher level at lactation period. Inhibition of FASN activity by different concentrations (0, 5, 15, 25 and 35 μM) of orlistat, a natural inhibitor of FASN, resulted in decreased expression of acetyl-CoA carboxylase α (ACCα), lipoprotein lipase and heart-type fatty acid binding protein (H-FABP) in a concentration-dependent manner in goat mammary gland epithelial cells (GMEC). Similar results were also obtained by silencing of FASN. Additionally, reduction of FASN expression also led to apparent decline of the relative content of decanoic acid (C10:0) and lauric acid (C12:0) in GMEC. Our study provides a direct evidence for inhibition of FASN reduces cellular medium-chain fatty acids synthesis in GMEC.
authors.affiliation: 1Shaanxi Key Laboratory of Molecular Biology for Agriculture,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,PR China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zhu
authors.affiliation: 1Shaanxi Key Laboratory of Molecular Biology for Agriculture,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,PR China.
authors.forename: J
authors.initials: J
authors.lastname: Luo
authors.affiliation: 2Department of Biological Sciences,University of Alabama,Tuscaloosa,USA.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.affiliation: 1Shaanxi Key Laboratory of Molecular Biology for Agriculture,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,PR China.
authors.forename: K
authors.initials: K
authors.lastname: Yu
authors.affiliation: 1Shaanxi Key Laboratory of Molecular Biology for Agriculture,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,PR China.
authors.forename: H B
authors.initials: HB
authors.lastname: Wang
authors.affiliation: 1Shaanxi Key Laboratory of Molecular Biology for Agriculture,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,PR China.
authors.forename: H B
authors.initials: HB
authors.lastname: Shi
authors.affiliation: 1Shaanxi Key Laboratory of Molecular Biology for Agriculture,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,PR China.
authors.forename: Y T
authors.initials: YT
authors.lastname: Sun
authors.affiliation: 1Shaanxi Key Laboratory of Molecular Biology for Agriculture,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,PR China.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Lin
authors.affiliation: 1Shaanxi Key Laboratory of Molecular Biology for Agriculture,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,PR China.
authors.forename: J
authors.initials: J
authors.lastname: Li
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000037:antagonists & inhibitors* / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006041:Goats / Q000378:metabolism*; D007774:Lactation; D007783:Lactones / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D000077403:Orlistat; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D005227:Fatty Acids; D007783:Lactones; D012313:RNA; D000077403:Orlistat; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase