title: Durable modification of segmented polyurethane for elastic blood-contacting devices by graft-type 2-methacryloyloxyethyl phosphorylcholine copolymer.
abstract: We propose a novel application of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers for enhancing the performance of modified segmented polyurethane (SPU) surfaces for the development of a small-diameter vascular prosthesis. The SPU membranes were modified by random-type, block-type, and graft-type MPC polymers that were prepared using a double-solution casting procedure on stainless steel substrates. Among these MPC polymers, the graft-type poly(MPC-graft-2-ethylhexyl methacrylate [EHMA]), which is composed of a poly(MPC) segment as the main chain and poly(EHMA) segments as side chains, indicated a higher stability on the SPU membrane after being peeled off from the stainless steel substrate, as well as after immersion in an aqueous medium. This stability was caused by the intermiscibility in the domain of the poly(EHMA) segments and the soft segments of the SPU membrane. Each SPU/MPC polymer membrane exhibited a dramatic suppression of protein adsorption from human plasma and endothelium cell adhesion. Based on these results, the performance of SPU/poly(MPC-graft-EHMA) tubings 2 mm in diameter as vascular prostheses was investigated. Even after blood was passed through the tubings for 2 min, the graft-type MPC polymers effectively protected the blood-contacting surfaces from thrombus formation. In summary, SPU modified by graft-type MPC polymers has the potential for practical application in the form of a non-endothelium, small-diameter vascular prosthesis.
authors.affiliation: a Department of Bioengineering , School of Engineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 , Japan.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Inoue
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mahara
authors.forename: Sachiro
authors.initials: S
authors.lastname: Kakinoki
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamaoka
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishihara
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D001798:Blood Proteins / Q000737:chemistry; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion / Q000187:drug effects; D004548:Elasticity; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008567:Membranes, Artificial; D008689:Methacrylates / Q000737:chemistry*; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology*; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D008567:Membranes, Artificial; D008689:Methacrylates; D011140:Polyurethanes; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine
keywords: 2-methacryloyloxyethyl phosphorylcholine polymer; antithrombogenicity; non-endothelium; protein adsorption; segmented polyurethane; surface modification