title: Physicochemical properties of newly developed bioactive glass cement and its effects on various cells.
abstract: Biomaterials used in dental treatments are expected to have favorable properties such as biocompatibility and an ability to induce tissue formation in dental pulp and periapical tissue, as well as sealing to block external stimuli. Bioactive glasses have been applied in bone engineering, but rarely applied in the field of dentistry. In the present study, bioactive glass cement for dental treatment was developed, and then its physicochemical properties and effects on cell responses were analyzed. To clarify the physicochemical attributes of the cement, field emission scanning electron microscopy, X-ray diffraction, and pH measurement were carried out. Cell attachment, morphology, and viability to the cement were also examined to clarify the effects of the cement on odontoblast-like cells (KN-3 cells), osteoblastic cells (MC3T3-E1 cells), human periodontal ligament stem/progenitor cells and neuro-differentiative cells (PC-12 cells). Hydroxyapatite-like precipitation was formed on the surface of the hardened cement and the pH level changed from pH10 to pH9, then stabilized in simulate body fluid. The cement had no cytotxic effects on these cells, and particulary induced process elongation of PC-12 cells. Our results suggest that the newly developed bioactive glass cement have capability of the application in dental procedures as bioactive cement.
authors.affiliation: Division of Endodontics and Restorative Dentistry, Department of Science of Oral Functions, Kyushu Dental University, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Washio
authors.forename: Aika
authors.initials: A
authors.lastname: Nakagawa
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Nishihara
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Maeda
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D003738:Dental Cements / Q000737:chemistry / Q000494:pharmacology; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D051379:Mice; D016716:PC12 Cells; D051381:Rats
chemical_list: D003738:Dental Cements
keywords: bioactive glass; biocompatibility; endodontic treatment; hydroxyapatite; pulp capping