title: Cutting edge: Dexamethasone potentiates the responses of both regulatory T cells and B-1 cells to antigen immunization in the ApoE(-/-) mouse model of atherosclerosis.
abstract: The immunosuppressant dexamethasone was shown to preferentially deplete CD4+ effector T cells while sparing regulatory T cells (Tregs) in vivo. In the current study, we show that it also preferentially depletes B-2 cells while sparing B-1 cells. In the ApoE(-/-) mouse model of atherosclerosis, in which both Tregs and B-1 cells are thought to play an atheroprotective role, we show that HSP60-targeted immunization in the presence of dexamethasone raises Ag-reactive Tregs and B-1 cells concomitantly and reduces the severity of atherosclerosis. These results indicate that dexamethasone is an adjuvant that potentiates both the Treg and B-1 responses to immunogens. This study shows that B-1 cells with a specificity for a disease-relevant Ag can be raised in vivo by immunization.
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107; aoshuang@uic.edu guoxingz@uic.edu.
authors.forename: Aoshuang
authors.initials: A
authors.lastname: Chen
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107;
authors.forename: Yajun
authors.initials: Y
authors.lastname: Geng
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107;
authors.forename: Hanzhong
authors.initials: H
authors.lastname: Ke
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107;
authors.forename: Laura
authors.initials: L
authors.lastname: Constant
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107;
authors.forename: Zhaoqi
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107;
authors.forename: Yue
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107;
authors.forename: Patricia
authors.initials: P
authors.lastname: Lee
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107;
authors.forename: Isaiah
authors.initials: I
authors.lastname: Tan
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107;
authors.forename: Kurt
authors.initials: K
authors.lastname: Williams
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107;
authors.forename: Samantha
authors.initials: S
authors.lastname: George
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107;
authors.forename: Gnanasekar
authors.initials: G
authors.lastname: Munirathinam
authors.affiliation: Department of Pathology, University of Chicago, Chicago, IL 60637; and.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Reardon
authors.affiliation: Department of Pathology, University of Chicago, Chicago, IL 60637; and.
authors.forename: Godfrey S
authors.initials: GS
authors.lastname: Getz
authors.affiliation: Key Laboratory of Medical Molecular Virology of Ministry of Health and Ministry of Education, Fudan University, Shanghai 200032, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107; aoshuang@uic.edu guoxingz@uic.edu.
authors.forename: Guoxing
authors.initials: G
authors.lastname: Zheng
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000941:Antigens / Q000494:pharmacology*; D001057:Apolipoproteins E; D050197:Atherosclerosis / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology; D003907:Dexamethasone / Q000494:pharmacology*; D004195:Disease Models, Animal; D007114:Immunization; D051379:Mice; D018345:Mice, Knockout; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000473:pathology
chemical_list: D000276:Adjuvants, Immunologic; D000893:Anti-Inflammatory Agents; D000941:Antigens; D001057:Apolipoproteins E; D003907:Dexamethasone