title: Revisiting guidelines for integration of flow cytometry results in the WHO classification of myelodysplastic syndromes-proposal from the International/European LeukemiaNet Working Group for Flow Cytometry in MDS.
abstract: Definite progress has been made in the exploration of myelodysplastic syndromes (MDS) by flow cytometry (FCM) since the publication of the World Health Organization 2008 classification of myeloid neoplasms. An international working party initiated within the European LeukemiaNet and extended to include members from Australia, Canada, Japan, Taiwan and the United States has, through several workshops, developed and subsequently published consensus recommendations. The latter deal with preanalytical precautions, and propose small and large panels, which allow evaluating immunophenotypic anomalies and calculating myelodysplasia scores. The current paper provides guidelines that strongly recommend the integration of FCM data with other diagnostic tools in the diagnostic work-up of MDS.
authors.affiliation: Department of Pathobiology and Laboratory Medicine, University of Toronto, University Health Network, Toronto General Hospital, Toronto, Ontario, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Porwit
authors.affiliation: Department of Hematology, Cancer Center Amsterdam, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: A A
authors.initials: AA
authors.lastname: van de Loosdrecht
authors.affiliation: First Medical Department, Elisabethinen Hospital, Linz, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Bettelheim
authors.affiliation: HematoLogics, Inc., Seattle, WA, USA.
authors.forename: L Eidenschink
authors.initials: LE
authors.lastname: Brodersen
authors.affiliation: Division of Cancer Medicine, Peter MacCallum Cancer Centre, East Melbourne, Melbourne, Victoria, Australia.
authors.forename: K
authors.initials: K
authors.lastname: Burbury
authors.affiliation: Department of Hematology, Cancer Center Amsterdam, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Cremers
authors.affiliation: Department of Hematology and Oncology, Fondazione IRCCS Policlinico San Matteo, Pavia, and University of Pavia, Pavia, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Della Porta
authors.affiliation: King's College Hospital, London, UK.
authors.forename: R
authors.initials: R
authors.lastname: Ireland
authors.affiliation: University Hospitals, Bristol, UK.
authors.forename: U
authors.initials: U
authors.lastname: Johansson
authors.affiliation: Servicio Central de Citometría, Centro de Investigación del Cáncer, Instituto de Biologia Celular y Molecular del Cáncer CSIC/USAL/IBSAL) and Department of Medicine, Universidad de Salamanca, Salamanca, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Matarraz
authors.affiliation: Metropolitan Research Center for Blood Disorders MRC JAPAN, Midorigaoka, Chofu, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ogata
authors.affiliation: Servicio Central de Citometría, Centro de Investigación del Cáncer, Instituto de Biologia Celular y Molecular del Cáncer CSIC/USAL/IBSAL) and Department of Medicine, Universidad de Salamanca, Salamanca, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Orfao
authors.affiliation: Department of Hematology, St Radboud University Medical Center, Nijmegen, The Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: Preijers
authors.affiliation: Department of Immunology-Histocompatibility, Evangelismos Hospital, Athens, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Psarra
authors.affiliation: Department of Hematology, Hospital Universitario de Guadalajara, Guadalajara, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Subirá
authors.affiliation: Department of Internal Medicine I, Division of Hematology and Hemostaseology and Ludwig Boltzmann Cluster Oncology, Medical University of Vienna, Vienna, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Valent
authors.affiliation: Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
authors.forename: V H J
authors.initials: VH
authors.lastname: van der Velden
authors.affiliation: HematoLogics, Inc., Seattle, WA, USA.
authors.forename: D
authors.initials: D
authors.lastname: Wells
authors.affiliation: Department of Hematology, Cancer Center Amsterdam, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: T M
authors.initials: TM
authors.lastname: Westers
authors.affiliation: MLL Munich Leukemia Laboratory, Munich, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Kern
authors.affiliation: Service d'Hématologie Biologique, CHU de Nantes, Nantes, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Béné
mesh_terms: D005060:Europe; D005434:Flow Cytometry / Q000379:methods*; D017408:Guidelines as Topic; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000145:classification* / Q000175:diagnosis; D014944:World Health Organization