title: Molecular characterization of Thai Salmonella enterica serotype typhimurium and serotype 4,5,12:i:- reveals distinct genetic deletion patterns.
abstract: In order to better understand the relationship between Salmonella serotypes Typhimurium and its monophasic variant 4,5,12:i:- found in Thailand, a total of 138 isolates from various sources were characterized using different molecular subtyping methods (i.e., pulsed-field gel electrophoresis [PFGE] and polymerase chain reaction [PCR]) and antibiotic resistance (AbR) patterns. PFGE revealed 52 distinct band patterns among these isolates, 3 of which were shared between the two serotypes. PCR characterization of genomic deletion patterns reveals that Thai S. 4,5,12:i:- isolates contain a distinct deletion pattern in the fljAB region, which can be used as a specific genetic marker for primary identification of S. 4,5,12:i:- sources. AbR study shows that, among 50 representative serotype-confirmed strains, 48.28% (14/29) of Salmonella Typhimurium and 90.48% (19/21) of 4,5,12:i:- isolates are multidrug-resistant Salmonella as they are resistant to at least 3 antimicrobial categories. The AmpST pattern for resistance to ampicillin, streptomycin, and tetracycline was found in high proportions of Salmonella Typhimurium (10 of 29) and S. 4,5,12:i:- (15 of 21) isolates.
authors.affiliation: 1 Department of Microbiology, Faculty of Science, Mahidol University , Bangkok, Thailand .
authors.forename: Laingshun
authors.initials: L
authors.lastname: Huoy
authors.forename: Srirat
authors.initials: S
authors.lastname: Pornruangwong
authors.forename: Chaiwat
authors.initials: C
authors.lastname: Pulsrikarn
authors.forename: Soraya
authors.initials: S
authors.lastname: Chaturongakul
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D017353:Gene Deletion; D005819:Genetic Markers; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000145:classification* / Q000187:drug effects / Q000235:genetics*; D065288:Serogroup; D013307:Streptomycin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D013785:Thailand
chemical_list: D000900:Anti-Bacterial Agents; D005819:Genetic Markers; D000667:Ampicillin; D013752:Tetracycline; D013307:Streptomycin