title: Environmental survivability and surface sampling efficiencies for Pseudomonas aeruginosa on various fomites.
abstract: The study described in this article evaluated surface survivability of culturable Pseudomonas aeruginosa by time and type (glass, stainless steel, and laminate) using two sampling techniques: contact plates and surface swabs. Recovery of P. aeruginosa decreased logarithmically over time and varied by surface type. P. aeruginosa survival averaged 3.75, 5.75, and 6.75 hours on laminate, glass, and stainless steel, respectively. Culturable P. aeruginosa loss on stainless steel and glass were not different (p > .05); however, laminate had significantly greater loss at each time point than either glass or stainless (p < .05). A comparison of surface swab and contact plate collection efficiencies found no significant difference for laminate surfaces. Swabs, however, had a higher collection efficiency than contact plates (p < .05). For the first time, the authors report P. aeruginosa mean survival time of 3.75-6.75 hours on clinically relevant surfaces, with P. aeruginosa on stainless steel surviving the longest. Their data also indicate that culturable surface sampling appears to most accurately represent actual P. aeruginosa surface loading when swab sampling is used.
authors.forename: Tia M
authors.initials: TM
authors.lastname: Jones
authors.forename: Eric A
authors.initials: EA
authors.lastname: Lutz
mesh_terms: D050456:Fomites / Q000382:microbiology*; D005898:Glass; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification* / Q000502:physiology; D013048:Specimen Handling / Q000379:methods*; D013193:Stainless Steel
chemical_list: D013193:Stainless Steel