title: Two new species of Lactarius associated with Alnus acuminata subsp. arguta in Mexico.
abstract: In pure stands of Alnus acuminata subsp. arguta trees from Sierra Norte de Puebla (central Mexico) two undescribed ectomycorrhizal species of Lactarius were discovered. Distinction of the two new species is based on morphological characters and supported with phylogenetic analyses of the nuclear ribosomal DNA ITS region and part of the gene that encodes for the second largest subunit of RNA polymerase II (rpb2). The phylogenies inferred recovered the two species in different clades strongly supported by posterior probabilities and bootstrap values. The new Lactarius species are recognized as part of the assemblage of ectomycorrhizal fungi associated with Alnus acuminata. Information about these taxa includes the morphological variation achieved along 16 monitories 2010-2013. Descriptions are provided. They are accompanied by photos including SEM photomicrographs of basidiospores and information on differences between them and other related taxa from Europe and the United States.
authors.affiliation: Biodiversidad y Sistemática, Instituto de Ecología, A.C., P.O. Box 63, Xalapa, Veracruz 91000, Mexico leticia.montoya@inecol.mx.
authors.forename: Leticia
authors.initials: L
authors.lastname: Montoya
authors.affiliation: Biodiversidad y Sistemática, Instituto de Ecología, A.C., P.O. Box 63, Xalapa, Veracruz 91000, Mexico.
authors.forename: Victor M
authors.initials: VM
authors.lastname: Bandala
authors.affiliation: Biodiversidad y Sistemática, Instituto de Ecología, A.C., P.O. Box 63, Xalapa, Veracruz 91000, Mexico.
authors.forename: Edith
authors.initials: E
authors.lastname: Garay
mesh_terms: D029661:Alnus / Q000382:microbiology*; D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D048690:Fruiting Bodies, Fungal; D005656:Fungal Proteins / Q000235:genetics; D008800:Mexico; D008855:Microscopy, Electron, Scanning; D038821:Mycorrhizae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D010802:Phylogeny; D012319:RNA Polymerase II / Q000235:genetics; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D012319:RNA Polymerase II
keywords: ITS; Neotropical fungi; Russulaceae; Russulales; ectomycorrhizal fungi; rpb2