title: Biomarkers of oxidative stress and inflammation after wood smoke exposure in a reconstructed Viking Age house.
abstract: Exposure to particles from combustion of wood is associated with respiratory symptoms, whereas there is limited knowledge about systemic effects. We investigated effects on systemic inflammation, oxidative stress and DNA damage in humans who lived in a reconstructed Viking Age house, with indoor combustion of wood for heating and cooking. The subjects were exposed to high indoor concentrations of PM2.5 (700-3,600 µg/m(3)), CO (10.7-15.3 ppm) and NO2 (140-154 µg/m(3)) during a 1-week stay. Nevertheless, there were unaltered levels of genotoxicity, determined as DNA strand breaks and formamidopyrimidine DNA glycosylase and oxoguanine DNA glycosylase 1 sensitive sites in peripheral blood mononuclear cells. There were also unaltered expression levels of OGG1, HMOX1, CCL2, IL8, and TNF levels in leukocytes. In serum, there were unaltered levels of C-reactive protein, IL6, IL8, TNF, lactate dehydrogenase, cholesterol, triglycerides, and high-density lipoproteins. The wood smoke exposure was associated with decreased serum levels of sICAM-1, and a tendency to decreased sVCAM-1 levels. There was a minor increase in the levels of circulating monocytes expressing CD31, whereas there were unaltered expression levels of CD11b, CD49d, and CD62L on monocytes after the stay in the house. In conclusion, even a high inhalation exposure to wood smoke was associated with limited systemic effects on markers of oxidative stress, DNA damage, inflammation, and monocyte activation.
authors.affiliation: Department of Public Health, Section of Environmental Health, University of Copenhagen, Denmark.
authors.forename: Annie
authors.initials: A
authors.lastname: Jensen
authors.forename: Dorina Gabriela
authors.initials: DG
authors.lastname: Karottki
authors.forename: Jannie Marie
authors.initials: JM
authors.lastname: Christensen
authors.forename: Jakob Hjort
authors.initials: JH
authors.lastname: Bønløkke
authors.forename: Torben
authors.initials: T
authors.lastname: Sigsgaard
authors.forename: Marianne
authors.initials: M
authors.lastname: Glasius
authors.forename: Steffen
authors.initials: S
authors.lastname: Loft
authors.forename: Peter
authors.initials: P
authors.lastname: Møller
mesh_terms: D000328:Adult; D016902:Air Pollution, Indoor / Q000009:adverse effects*; D015415:Biomarkers / Q000032:analysis*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006798:Housing; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D010316:Particle Size; D052638:Particulate Matter / Q000633:toxicity; D012907:Smoking; D018605:Spontaneous Combustion; D014934:Wood; D055815:Young Adult
chemical_list: D015415:Biomarkers; D052638:Particulate Matter
keywords: DNA damage; comet assay; inflammation; oxidative stress; particulate matter; wood smoke