title: Immunostimulatory effects of Leishmania infantum HSP70 recombinant protein on dendritic cells in vitro and in vivo.
abstract: BACKGROUND
  Activation of dendritic cells (DCs) has an important role in immunity against Leishmania.
  
  
  OBJECTIVE
  We investigated the effect of Leishmania infantum (L. infantum) heat shock protein 70 recombinant protein (rHSP70) as a vaccine on DC maturation and function.
  
  
  METHODS
  BALB/c mouse splenic DCs were isolated and treated with different concentrations of rHSP70. Maturation markers, cytokine production and capability of DCs to proliferate allogeneic T cells were evaluated. Furthermore, this recombinant protein was injected into BALB/c mice, and expression of CD86, CD40 and MHC class II molecules by their splenic DCs were evaluated.
  
  
  RESULTS
  rHSP70 significantly increases the production of IL-12p70 by DCs. It had no effect on allogeneic T-cell proliferation in mixed lymphocyte reaction. It increased IFN-γ and decreased IL-4 cytokine level in mixed lymphocyte reaction supernatant. The in vitro study showed that rHSP70 had no significant effect neither on the percentage of CD40(+), CD86(+) and MHC class II(+) DCs nor on the mean fluorescent intensity. However, in vivo results showed that rHSP70 increases the percentage of CD86-, CD40- and MHC class II-expressing cells as well as mean fluorescent intensity of CD40 and MHC class II.
  
  
  CONCLUSIONS
  This study demonstrated the capability of L. infantum-derived rHSP70 in maturating BALB/c mice splenic DCs and in vivo polarization of immunity to a Th1 response.
authors.affiliation: Department of Immunology, Professor Alborzi Clinical Microbiology Research Center, Namazi Hospital, Shiraz, Iran.
authors.forename: Manoochehr
authors.initials: M
authors.lastname: Rasouli
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Karimi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Kalani
authors.forename: Salimeh
authors.initials: S
authors.lastname: Ebrahimnezhad
authors.forename: Manadana
authors.initials: M
authors.lastname: Namayandeh
authors.forename: Ali
authors.initials: A
authors.lastname: Moravej
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D051940:B7-2 Antigen / Q000276:immunology; D019013:CD40 Antigens / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D018314:Leishmania infantum / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
chemical_list: D000276:Adjuvants, Immunologic; D051940:B7-2 Antigen; D019013:CD40 Antigens; C497965:Cd86 protein, mouse; D018840:HSP70 Heat-Shock Proteins; D000949:Histocompatibility Antigens Class II; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D018664:Interleukin-12; D015847:Interleukin-4
keywords: Leishmania infantum; dendritic cell; heat shock protein 70; immunostimulation