title: Apoptosis-like events and in vitro fertilization capacity of sex-sorted bovine sperm.
abstract: This study utilized three staining assays (Annexin V, mitochondrial membrane potential (JC-1) and TUNEL) for flow cytometric analysis of apoptosis in sex-sorted sperm from four different bulls (A, B, C and D). Correlations between sperm quality and IVF efficiency were then assessed to determine which assay provided the best prediction of IVF efficiency. The results of the Annexin V assays, as well as measures of viable sperm, early apoptosis, necrotic sperm and mitochondrial membrane potential (∆ψm) showed that the sex-sorted sperm collected from bull A significantly differed from those of the other three bulls (p < 0.05). In addition, the levels of DNA fragmentation in sex-sorted sperm from bull A were significantly lower than those from bulls B and C (p < 0.05). The percentage of cells reaching the cleavage and blastocyst stages in sex-sorted sperm from bull A were significantly greater than those from the other bulls (p < 0.05). A significant positive correlation was observed between viable sperm and the percentage of cells at the cleavage or blastocyst stages (p < 0.05). In contrast, a negative correlation was found between early apoptotic sperm and the percentage of cells at the cleavage or blastocyst stages (p < 0.05). In conclusion, these results indicated that the Annexin V assay was the most reliable technique for the prediction of the IVF success of sex-sorted bovine sperm.
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: X-M
authors.initials: XM
authors.lastname: Zhao
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: J-J
authors.initials: JJ
authors.lastname: Ren
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: S-J
authors.initials: SJ
authors.lastname: Zhao
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: L-S
authors.initials: LS
authors.lastname: Cui
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: H-S
authors.initials: HS
authors.lastname: Hao
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: H-Y
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: W-H
authors.initials: WH
authors.lastname: Du
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: T
authors.initials: T
authors.lastname: Qin
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: D
authors.initials: D
authors.lastname: Wang
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: H-B
authors.initials: HB
authors.lastname: Zhu
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis; D001755:Blastocyst; D002417:Cattle; D002469:Cell Separation; D002970:Cleavage Stage, Ovum; D053938:DNA Fragmentation; D047108:Embryonic Development; D005307:Fertilization in Vitro / Q000662:veterinary*; D005434:Flow Cytometry; D020287:In Situ Nick-End Labeling; D008297:Male; D012743:Sex Preselection / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000737:chemistry / Q000166:cytology* / Q000502:physiology*
chemical_list: D017304:Annexin A5