title: Mapping-by-sequencing accelerates forward genetics in barley.
abstract: Mapping-by-sequencing has emerged as a powerful technique for genetic mapping in several plant and animal species. As this resequencing-based method requires a reference genome, its application to complex plant genomes with incomplete and fragmented sequence resources remains challenging. We perform exome sequencing of phenotypic bulks of a mapping population of barley segregating for a mutant phenotype that increases the rate of leaf initiation. Read depth analysis identifies a candidate gene, which is confirmed by the analysis of independent mutant alleles. Our method illustrates how the genomic resources of barley together with exome resequencing can underpin mapping-by-sequencing.
authors.forename: Martin
authors.initials: M
authors.lastname: Mascher
authors.forename: Matthias
authors.initials: M
authors.lastname: Jost
authors.forename: Joel-Elias
authors.initials: JE
authors.lastname: Kuon
authors.forename: Axel
authors.initials: A
authors.lastname: Himmelbach
authors.forename: Axel
authors.initials: A
authors.lastname: Aßfalg
authors.forename: Sebastian
authors.initials: S
authors.lastname: Beier
authors.forename: Uwe
authors.initials: U
authors.lastname: Scholz
authors.forename: Andreas
authors.initials: A
authors.lastname: Graner
authors.forename: Nils
authors.initials: N
authors.lastname: Stein
mesh_terms: D002874:Chromosome Mapping; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D005787:Gene Frequency; D017343:Genes, Plant; D056726:Genetic Association Studies; D056426:Genetic Loci; D001467:Hordeum / Q000235:genetics*; D016296:Mutagenesis; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System