title: Beneficial rhizobacteria from rice rhizosphere confers high protection against biotic and abiotic stress inducing systemic resistance in rice seedlings.
abstract: The present study reports a screening for PGPR in a highly selective environment, the rhizosphere of rice plants, in southwestern of Spain. Among the 900 isolates, only 38% were positive for at least one of the biochemical activities to detect putative PGPR. The best 80 isolates were selected and identified by 16S rRNA partial sequencing. Among these, 13 strains were selected for growth promotion assays. Only one strain (BaC1-38) was able to significantly increase height, while nine strains significantly inhibited it. Five strains significantly increased dry weight, and only BaC1-21 significantly decreased it. Based on significant modifications in growth, three bacteria (BaC1-13, BaC1-21 and BaC1-38) were tested for systemic induction of resistance against stress challenge (salt and Xanthomonas campestris infection). Protection against salt stress and pathogen infection was similar; BaC1-38 protected by 80%, BaC1-13 by 50% and BaC1-21 only by 20%. Toxicity of salt stress to the plants was evaluated by photosynthetic efficiency of seedlings. Fv/Fm only decreased significantly in plants inoculated with BaC1-13. ΦPSII also decreased significantly in plants inoculated with BaC1-21, but increased significantly with BaC1-38. NPQ decreased significantly in plants inoculated with BaC1-21. The two strains able to induce systemic resistance against Xanthomonas campestris seem to work by different pathways. BaC1-13 primed enzymes related with the detoxification of reactive oxygen species (ROS). However, BaC1-38 primed pathogenesis-related proteins (PRs), and this pathway was more effective, both improved chlorophyll index confirming the priming state of the plant.
authors.affiliation: Universidad San Pablo CEU. Dept. Pharmaceutical & Health Sciences, Facultad Farmacia, Urb. Monteprincipe, Boadilla del Monte, 28668 Madrid, Spain. Electronic address: alucgar@ceu.es.
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Lucas
authors.affiliation: Universidad San Pablo CEU. Dept. Pharmaceutical & Health Sciences, Facultad Farmacia, Urb. Monteprincipe, Boadilla del Monte, 28668 Madrid, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: García-Cristobal
authors.affiliation: Universidad San Pablo CEU. Dept. Pharmaceutical & Health Sciences, Facultad Farmacia, Urb. Monteprincipe, Boadilla del Monte, 28668 Madrid, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Bonilla
authors.affiliation: Universidad San Pablo CEU. Dept. Pharmaceutical & Health Sciences, Facultad Farmacia, Urb. Monteprincipe, Boadilla del Monte, 28668 Madrid, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Ramos
authors.affiliation: Universidad San Pablo CEU. Dept. Pharmaceutical & Health Sciences, Facultad Farmacia, Urb. Monteprincipe, Boadilla del Monte, 28668 Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Gutierrez-Mañero
mesh_terms: D012275:Oryza / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D058441:Rhizosphere; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D012965:Sodium Chloride
keywords: Induced systemic resistance (ISR); Oryza sativa; Oxidative stress related enzymes; PGPR; Pathogenesis-related proteins (PRs); Priming; Salt stress