title: Adipokinetic hormone induces changes in the fat body lipid composition of the beetle Zophobas atratus.
abstract: In insects, neuropeptide adipokinetic hormone (AKH) released from the corpora cardiaca mobilizes lipids and carbohydrates in the fat body. We examined the developmental differences in the action of Tenmo-AKH, a bioanalogue belonging to the adipokinetic/hypertrahelosemic family (AKH/HrTH), on the lipid composition of larval and pupal fat bodies in the beetle Zophobas atratus. Tenmo-AKH was administered to the beetle larvae and pupae either as a single dose or as two doses of 20 pmol during a 24h interval. Extracts of fat bodies were used to analyse the lipid composition by gas chromatography (GC) combined with mass spectrometry (GC-MS). Control extracts were analyzed using the same method. Fatty acids (FA) and fatty acid methyl esters (FAME) were the most abundant compounds in the fat bodies from both developmental stages. We observed significant differences in their concentrations following hormonal treatment. Tenmo-AKH also induced a distinct increase in larval sterols, fatty alcohols and benzoic acid.
authors.affiliation: Laboratory of Natural Products Analysis, Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdansk, ul. Wita Stwosza 63, 80-308, Poland. Electronic address: marek.golebiowski@ug.edu.pl.
authors.forename: Marek
authors.initials: M
authors.lastname: Gołębiowski
authors.affiliation: Laboratory of Natural Products Analysis, Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdansk, ul. Wita Stwosza 63, 80-308, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Cerkowniak
authors.affiliation: Department of Invertebrate Zoology and Parasitology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Urbanek
authors.affiliation: Department of Animal Physiology and Development, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Słocińska
authors.affiliation: Department of Animal Physiology and Development, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Rosiński
authors.affiliation: Laboratory of Natural Products Analysis, Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdansk, ul. Wita Stwosza 63, 80-308, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Stepnowski
mesh_terms: D054392:Adipokines / Q000494:pharmacology*; D000818:Animals; D001517:Coleoptera / Q000378:metabolism*; D005216:Fat Body / Q000378:metabolism*; D019476:Insect Proteins / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*
chemical_list: D054392:Adipokines; D019476:Insect Proteins
keywords: Adipokinetic hormone; Fat body; GC–MS; Lipids; Zophobas atratus