title: nNOS polymorphisms are associated with responsiveness to sildenafil in clinical and postoperative erectile dysfunction.
abstract: OBJECTIVE
  Sildenafil potentiates the nitric oxide (NO) signaling pathway. Since neuronal NOS is very important in the penis, we assessed whether NOS1 polymorphisms are associated with altered responsiveness to sildenafil in erectile dysfunction (ED).
  
  
  METHODS
  Patients (n = 137) were divided as clinical ED or postoperative ED. They were subdivided as good responders or poor responders to sildenafil, and genotypes for rs41279104 and rs2682826 NOS1 polymorphisms were determined.
  
  
  RESULTS
  We found that the rs41279104 CT genotype was associated with good responders in postoperative ED patients, while rs2682826 CT genotype was associated with good responders in postoperative ED, and the TT genotype associated with good responders in both groups. Finally, the CT haplotype was associated with good responders in postoperative ED.
  
  
  CONCLUSIONS
  NOS1 polymorphisms are associated with responsiveness to sildenafil in ED. Original submitted 20 November 2013; Revision submitted 31 January 2014.
authors.affiliation: Department of Pharmacology, Ribeirao Preto Medical School, University of Sao Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirao Preto, SP, Brazil. tanus@fmrp.usp.br.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Lacchini
authors.forename: Jaqueline J
authors.initials: JJ
authors.lastname: Muniz
authors.forename: Yuri T D A
authors.initials: YT
authors.lastname: Nobre
authors.forename: Adauto J
authors.initials: AJ
authors.lastname: Cologna
authors.forename: Antonio C P
authors.initials: AC
authors.lastname: Martins
authors.forename: Jose E
authors.initials: JE
authors.lastname: Tanus-Santos
mesh_terms: D007172:Erectile Dysfunction / Q000188:drug therapy* / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D052248:Nitric Oxide Synthase Type I / Q000235:genetics*; D010879:Piperazines / Q000627:therapeutic use*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011184:Postoperative Period; D011687:Purines / Q000627:therapeutic use; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000627:therapeutic use*
chemical_list: D010879:Piperazines; D011687:Purines; D013450:Sulfones; D000068677:Sildenafil Citrate; C496320:NOS1 protein, human; D052248:Nitric Oxide Synthase Type I
keywords: NOS1; erectile dysfunction; nitric oxide; polymorphisms; prostatectomy; sildenafil