title: Analyzing sister chromatid cohesion in mammalian cells.
abstract: The metaphase chromosome spread technique and subsequent analysis of sister chromatid cohesion is used for (clinical) diagnosis of genetic abnormalities that can cause aberrant sister chromatid cohesion. In addition, the technique can be used to assess the contribution of novel genes to the cohesion establishment and maintenance pathways. Cells are swelled in a hypotonic solution and fixed in Carnoy's solution. Samples are then dropped onto glass slides, and the spread chromosomes are stained and visualized by microscopy. Defects in sister chromatid cohesion can be easily assessed using this method, examples of which are given.
authors.affiliation: School of Cancer Sciences, University of Birmingham, IBR West Extension WX1.24, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Feeney
authors.forename: Laura
authors.initials: L
authors.lastname: McFarlane-Majeed
authors.forename: Joanna L
authors.initials: JL
authors.lastname: Parish
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D002842:Chromatids / Q000378:metabolism* / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D006801:Humans; D008677:Metaphase; D008853:Microscopy / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000097722:Cohesins