title: MicroRNA-135a acts as a putative tumor suppressor by directly targeting very low density lipoprotein receptor in human gallbladder cancer.
abstract: The precise functions and mechanisms of microRNAs (miR) in gallbladder cancer (GBC) remain elusive. In this study, we found that miR-135a-5p expression is often dampened and correlated with neoplasm histologic grade in GBC. MicroRNA-135a-5p introduction clearly inhibited GBC cell proliferation in vitro and in vivo. Moreover, very low density lipoprotein receptor (VLDLR), which is often upregulated in GBC tissues, was identified as a direct functional target of miR-135a-5p. Furthermore, the p38 MAPK pathway was proven to be involved in miR-135a-VLDLR downstream signaling. Together, these results suggested that the miR-135a-VLDLR-p38 axis may contribute to GBC cell proliferation.
authors.affiliation: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Huading
authors.initials: H
authors.lastname: Zhou
authors.forename: Weijie
authors.initials: W
authors.lastname: Guo
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yifei
authors.initials: Y
authors.lastname: Wang
authors.forename: Ruopeng
authors.initials: R
authors.lastname: Zha
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Linhui
authors.initials: L
authors.lastname: Liang
authors.forename: Guanghua
authors.initials: G
authors.lastname: Yang
authors.forename: Zongyou
authors.initials: Z
authors.lastname: Chen
authors.forename: Baojin
authors.initials: B
authors.lastname: Ma
authors.forename: Baobing
authors.initials: B
authors.lastname: Yin
mesh_terms: D000368:Aged; D015153:Blotting, Western; D005260:Female; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D016147:Genes, Tumor Suppressor; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D011973:Receptors, LDL / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: C544965:MIRN135 microRNA, human; D035683:MicroRNAs; D011973:Receptors, LDL; C036053:VLDL receptor
keywords: Gallbladder cancer; MiR-135a; p38; proliferation; very low density lipoprotein receptor