title: A novel mycovirus closely related to viruses in the genus Alphapartitivirus confers hypovirulence in the phytopathogenic fungus Rhizoctonia solani.
abstract: We report here the biological and molecular attributes of a novel dsRNA mycovirus designated Rhizoctonia solani partitivirus 2 (RsPV2) from strain GD-11 of R. solani AG-1 IA, the causal agent of rice sheath blight. The RsPV2 genome comprises two dsRNAs, each possessing a single ORF. Phylogenetic analyses indicated that this novel virus species RsPV2 showed a high sequence identity with the members of genus Alphapartitivirus in the family Partitiviridae, and formed a distinct clade distantly related to the other genera of Partitiviridae. Introduction of purified RsPV2 virus particles into protoplasts of a virus-free virulent strain GD-118 of R. solani AG-1 IA resulted in a derivative isogenic strain GD-118T with reduced mycelial growth and hypovirulence to rice leaves. Taken together, it is concluded that RsPV2 is a novel dsRNA virus belonging to Alphapartitivirus, with potential role in biological control of R. solani.
authors.affiliation: Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, China.
authors.forename: Qiguang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, China.
authors.forename: Minghai
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, China. Electronic address: exzhou@scau.edu.cn.
authors.forename: Erxun
authors.initials: E
authors.lastname: Zhou
mesh_terms: D016000:Cluster Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D025282:Mycelium / Q000254:growth & development; D016366:Open Reading Frames; D012275:Oryza / Q000382:microbiology; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics*; D012232:Rhizoctonia / Q000254:growth & development / Q000472:pathogenicity* / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral
keywords: Alphapartitivirus; Biological control; Hypovirulence; Mycovirus; Rhizoctonia solani