title: Native chemical ubiquitination using a genetically incorporated azidonorleucine.
abstract: A robust chemical ubiquitination method was developed. The method employed a genetically incorporated azidonorleucine as an orthogonal lysine precursor for the installation of a Gly residue bearing an Nα-auxiliary which mediated the ligation between ubiquitin(1-75)-thioester and the target protein. To demonstrate our methodology, a model protein, K48-linked diubiquitin, was synthesized with an overall yield of 35%.
authors.affiliation: Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551. cfliu@ntu.edu.sg.
authors.forename: Renliang
authors.initials: R
authors.lastname: Yang
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Bi
authors.forename: Fupeng
authors.initials: F
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cao
authors.forename: Chuan-Fa
authors.initials: CF
authors.lastname: Liu
mesh_terms: D001386:Azides / Q000737:chemistry*; D015394:Molecular Structure; D009646:Norleucine / Q000031:analogs & derivatives* / Q000737:chemistry; D054875:Ubiquitination; D014452:Ubiquitins / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D001386:Azides; D014452:Ubiquitins; D009646:Norleucine