title: A phage-displayed peptide recognizing porcine aminopeptidase N is a potent small molecule inhibitor of PEDV entry.
abstract: Three phage-displayed peptides designated H, S and F that recognize porcine aminopeptidase N (pAPN), the cellular receptor of porcine transmissible gastroenteritis virus (TGEV) were able to inhibit cell infection by TGEV. These same peptides had no inhibitory effects on infection of Vero cells by porcine epidemic diarrhea virus (PEDV). However, when PEDV, TGEV and porcine pseudorabies virus were incubated with peptide H (HVTTTFAPPPPR), only infection of Vero cells by PEDV was inhibited. Immunofluoresence assays indicated that inhibition of PEDV infection by peptide H was independent of pAPN. Western blots demonstrated that peptide H interacted with PEDV spike protein and that pre-treatment of PEDV with peptide H led to a higher inhibition than synchronous incubation with cells. These results indicate direct interaction with the virus is necessary to inhibit infectivity. Temperature shift assays demonstrated that peptide H inhibited pre-attachment of the virus to the cells.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.
authors.forename: Fandan
authors.initials: F
authors.lastname: Meng
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.
authors.forename: Siqingaowa
authors.initials: S
authors.lastname: Suo
authors.affiliation: Animal Parasitic Diseases Laboratory, Agricultural Research Service, Beltsville, Maryland, USA.
authors.forename: Dante S
authors.initials: DS
authors.lastname: Zarlenga
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Cong
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China. Electronic address: rxfemail@gmail.com.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000378:metabolism*; D018826:CD13 Antigens / Q000378:metabolism*; D002522:Chlorocebus aethiops; D011558:Herpesvirus 1, Suid / Q000187:drug effects / Q000502:physiology; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*; D053485:Porcine epidemic diarrhea virus / Q000187:drug effects* / Q000502:physiology*; D005760:Transmissible gastroenteritis virus / Q000187:drug effects / Q000502:physiology; D014709:Vero Cells; D053586:Virus Internalization / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D019151:Peptide Library; D010455:Peptides; D018826:CD13 Antigens
keywords: Coronavirus; PEDV; Peptides; Virus attachment