title: Application of a collagen-based membrane and chondroitin sulfate-based hydrogel adhesive for the potential repair of severe ocular surface injuries.
abstract: This study was performed to evaluate the potential of a chondroitin sulfate-polyethylene glycol (CS-PEG) adhesive and collagen-based membrane (collagen vitrigel, CV) combination as a method to treat penetrating ocular injuries on the battlefield and to improve this method with two technologies: an antibiotic releasing CS-PEG adhesive and a corneal shaped CV. Burst testing using porcine cadaveric eyes, high-performance liquid chromatography, the Kirby-Bauer bacterial inhibition test, and CV implantations on the live and cadaveric rabbit eyes were performed. The ocular burst test showed CS-PEG adhesive could successfully repair 5-mm to 6-mm length wounds in the corneal and corneoscleral regions but would require CS-PEG + CV to treat larger wounds similar to those seen on the battlefield. In addition, high performance liquid chromatography and the Kirby-Bauer bacterial inhibition test presented evidence suggesting the vancomycin incorporated CS-PEG could inhibit Staphylococcus infection for 9 days. Furthermore, the curved CV showed an advantage by matching the corneal contour without any wrinkle formation. Although this pilot study showed a limited range of possible applications, we demonstrated that the combination of CS-PEG adhesive + CV is a promising method and the 2 technologies improve their applicability to the special demands of the battlefield.
authors.affiliation: Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287.
authors.forename: Jemin J
authors.initials: JJ
authors.lastname: Chae
authors.affiliation: Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287.
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Mulreany
authors.affiliation: Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287.
authors.forename: Qiongyu
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287.
authors.forename: Qiaozhi
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287.
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287.
authors.forename: Iossif
authors.initials: I
authors.lastname: Strehin
authors.affiliation: Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287.
authors.forename: Freddy A
authors.initials: FA
authors.lastname: Espinoza
authors.affiliation: Department of Ophthalmology, Johns Hopkins University, 600 N. Wolfe Street, Baltimore, MD 21287.
authors.forename: Oliver
authors.initials: O
authors.lastname: Schein
authors.affiliation: Research and Exploratory Development Department, Johns Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins Road, Laurel, MD 20723.
authors.forename: Morgana M
authors.initials: MM
authors.lastname: Trexler
authors.affiliation: Department of Ophthalmology, Johns Hopkins University, 600 N. Wolfe Street, Baltimore, MD 21287.
authors.forename: Kraig S
authors.initials: KS
authors.lastname: Bower
authors.affiliation: Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287.
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Elisseeff
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001753:Blast Injuries / Q000628:therapy*; D002102:Cadaver; D002809:Chondroitin Sulfates / Q000627:therapeutic use*; D003094:Collagen / Q000627:therapeutic use*; D057112:Corneal Perforation / Q000628:therapy*; D052978:Disk Diffusion Antimicrobial Tests; D015807:Eye Injuries, Penetrating / Q000628:therapy*; D020100:Hydrogels; D008297:Male; D008567:Membranes, Artificial; D011092:Polyethylene Glycols / Q000627:therapeutic use*; D011817:Rabbits; D013211:Staphylococcus aureus / Q000187:drug effects; D013552:Swine; D014014:Tissue Adhesives / Q000627:therapeutic use; D014640:Vancomycin / Q000008:administration & dosage
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D008567:Membranes, Artificial; D014014:Tissue Adhesives; D011092:Polyethylene Glycols; D014640:Vancomycin; D002809:Chondroitin Sulfates; D003094:Collagen