title: Microfluidic techniques for development of 3D vascularized tissue.
abstract: Development of a vascularized tissue is one of the key challenges for the successful clinical application of tissue engineered constructs. Despite the significant efforts over the last few decades, establishing a gold standard to develop three dimensional (3D) vascularized tissues has still remained far from reality. Recent advances in the application of microfluidic platforms to the field of tissue engineering have greatly accelerated the progress toward the development of viable vascularized tissue constructs. Numerous techniques have emerged to induce the formation of vascular structure within tissues which can be broadly classified into two distinct categories, namely (1) prevascularization-based techniques and (2) vasculogenesis and angiogenesis-based techniques. This review presents an overview of the recent advancements in the vascularization techniques using both approaches for generating 3D vascular structure on microfluidic platforms.
authors.affiliation: Biomedical Engineering, and Department of Mechanical Engineering, American University of Beirut, Beirut 1107 2020, Lebanon; Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: mh211@aub.edu.lb.
authors.forename: Anwarul
authors.initials: A
authors.lastname: Hasan
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
authors.forename: Arghya
authors.initials: A
authors.lastname: Paul
authors.affiliation: INSERM, UMR-S 1121, Biomatériaux et Bioingénierie, 11 rue Humann, F-67085 Strasbourg Cedex, France.
authors.forename: Nihal E
authors.initials: NE
authors.lastname: Vrana
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Center of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Adnan
authors.initials: A
authors.lastname: Memic
authors.affiliation: Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Yu-Shik
authors.initials: YS
authors.lastname: Hwang
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Mehmet R
authors.initials: MR
authors.lastname: Dokmeci
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA; World Premier International - Advanced Institute for Materials Research (WPI-AIMR), Tohoku University, Sendai 980-8577, Japan; Department of Physics, King Abdulaziz University, Jeddah 21589, Saudi Arabia. Electronic address: alik@rics.bwh.harvard.edu.
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology / Q000502:physiology*; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D018919:Neovascularization, Physiologic; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods
keywords: Angiogenesis; Microfluidics; Micromolding; Tissue engineering; Vascularization; Vasculogenesis