title: Effect of recombinant hPTEN gene expression on PDGF induced VSMC proliferation.
abstract: Human phosphatase and tensin homolog (hPTEN) gene was expressed in vascular smooth muscle cells (VSMCs) to study its effect on VSMC proliferation induced in platelet-derived growth factor (PDGF) conditioned medium. After G418 selection, MTT assay was conducted to examine transfected VSMC proliferation induced in human PDGF conditioned medium. We successfully constructed eukaryotic expression vector pcDNA4/myc-His-PTEN and transferred into VSMC cells. We report that in vitro proliferation of VSMC was inhibited in PTEN transfected VSMCs induced in PDGF conditioned medium. RT-PCR and Western blot results indicated significantly high levels of protein kinase B-PKB and nuclear factor kappa B mRNA and protein, respectively, in PDGF group as compared with the control group.
authors.affiliation: Department of Oncology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, 130041, People's Republic of China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Hu
authors.forename: Shu
authors.initials: S
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Guang
authors.initials: G
authors.lastname: Shi
authors.forename: Yarong
authors.initials: Y
authors.lastname: Li
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D008081:Liposomes; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects*; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics*
chemical_list: D008081:Liposomes; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D051059:PTEN Phosphohydrolase