title: Description of Brenneria roseae sp. nov. and two subspecies, Brenneria roseae subspecies roseae ssp. nov and Brenneria roseae subspecies americana ssp. nov. isolated from symptomatic oak.
abstract: Gram-negative, facultatively anaerobic bacteria were isolated from symptomatic oak tissue in the UK and USA. Partial gyrB sequencing placed ten strains in the genus Brenneria, with B. goodwinii as the closest phylogenetic relative. The strains were investigated further using a polyphasic approach including MLSA (based on partial gyrB, rpoB, infB and atpD gene sequences), 16S rRNA gene sequencing, DNA-DNA relatedness studies and both phenotypic and chemotaxonomic assays. The MLSA and 16S rRNA gene analyses separated the strains into two groups based on origin, suggesting that they belong to Brenneria as two novel species. However, the DNA-DNA relatedness values revealed a closer relationship between the groups and indicated that they should belong to the same species. As the two groups of strains from the UK and USA can be differentiated from each other phenotypically and by ERIC PCR fingerprints, it is proposed to classify them as novel subspecies of a novel Brenneria species. The name Brenneria roseae sp. nov. (FRB 222(T)=LMG 27714(T)=NCPPB 4581(T)) is proposed, with Brenneria roseae subsp. roseae ssp. nov. (FRB 222(T)=LMG 27714(T)=NCPPB 4581(T)) for the strains from the UK and Brenneria roseae subsp. americana ssp. nov. (FRB 223(T)=LMG 27715(T)=NCPPB 4582(T)) for the strains from the USA.
authors.affiliation: Centre for Research in Bioscience, Faculty of Health and Life Sciences, University of the West of England, Frenchay Campus, Bristol, United Kingdom. Electronic address: carrie.brady@uwe.ac.uk.
authors.forename: Carrie
authors.initials: C
authors.lastname: Brady
authors.affiliation: Forest Research, Centre for Ecosystems Society and Biosecurity, Alice Holt Lodge, Surrey, United Kingdom.
authors.forename: Gavin
authors.initials: G
authors.lastname: Hunter
authors.affiliation: Forest Research, Centre for Ecosystems Society and Biosecurity, Alice Holt Lodge, Surrey, United Kingdom.
authors.forename: Susan
authors.initials: S
authors.lastname: Kirk
authors.affiliation: Centre for Research in Bioscience, Faculty of Health and Life Sciences, University of the West of England, Frenchay Campus, Bristol, United Kingdom.
authors.forename: Dawn
authors.initials: D
authors.lastname: Arnold
authors.affiliation: Forest Research, Centre for Ecosystems Society and Biosecurity, Alice Holt Lodge, Surrey, United Kingdom.
authors.forename: Sandra
authors.initials: S
authors.lastname: Denman
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D029963:Quercus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Acute Oak Decline; Brenneria; Enterobacteriaceae; MLSA; Taxonomy