title: Detection and characterization of avastrovirus associated with diarrhea isolated from minks in China.
abstract: Astroviruses are becoming a growing concern in veterinary and public health. Many astrovirus species are associated with enteric diseases have been described in both mammalian and avian hosts. In the present study, 23 fecal samples from diarrheic minks were collected in Liaoning and Shandong Province, and an investigation of astrovirus was performed using biochemical methods and RT-PCR assay with specific primers. A total of four mink astroviral isolates were detected from sick minks with diarrhea problems. Further sequencing and characterization of the partial ORF1b gene and ORF2 gene segments revealed low sequence identities (20.0-85.3 and 31.8-87.2%) with known astroviral strains, indicating the emergence of a novel clade of astroviruses. Some new features of the astroviral genome have also been discovered. The phylogenetic tree revealed that all samples were distantly related to mink astrovirus and were closely related to chicken astroviruses and turkey astroviruses. MK/DL-1, MK/DL-2, MK/SD-1, and MK/SD-2 formed a new clade and were found to be more closely related to astroviruses from birds than to other mink strains, indicating past cross-species transmission and considerable zoonotic potential.
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants Sciences, Chinese Academy of Agricultural Sciences CAAS, No. 4899, Juye Street, Jingyue Economic Development Zone, Changchun, 130112, China.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Gui-Sheng
authors.initials: GS
authors.lastname: Wang
authors.forename: Xi-Qun
authors.initials: XQ
authors.lastname: Shao
authors.forename: Shu-Qin
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Feng-Xue
authors.initials: FX
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Tan
authors.forename: Fu-Lin
authors.initials: FL
authors.lastname: Tian
authors.forename: Shi-Peng
authors.initials: SP
authors.lastname: Cheng
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Wen
mesh_terms: D000818:Animals; D019350:Astroviridae Infections / Q000662:veterinary* / Q000821:virology; D052676:Avastrovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D008907:Mink / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011200:Poultry; D011201:Poultry Diseases / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins