title: Effects of obesity/fatty acids on the expression of GPR120.
abstract: METHODS
  The effects that fatty acids (FAs) exert on G protein-coupled receptor-120 (GPR120) levels, a receptor for FAs, are still unknown. We analyzed the association between GPR120 and obesity, and the FA effects on its expression.
  
  
  RESULTS
  GPR120 levels were analyzed in visceral adipose tissue (VAT) from nonobese and morbidly obese subject. VAT GPR120 mRNA and protein levels were lower in morbidly obese subjects (p = 0.004). After, these subjects underwent a high-fat meal. GPR120 mRNA expression in peripheral blood mononuclear cells in the fasting state was lower in morbidly obese subjects (p = 0.04), with a decrease 3 h after a high-fat meal only in morbidly obese subjects (p = 0.043). Also, incubations of visceral adipocytes from these subjects were made with different FAs. In nonobese subjects, palmitic, oleic, linoleic, and docosahexaenoic acids produced an increase in GPR120 mRNA and protein levels (p < 0.05). In morbidly obese subjects, only linoleic acid produced an increase in GPR120 mRNA and protein levels (p < 0.05).
  
  
  CONCLUSIONS
  Morbidly obese subjects had lower GPR120 mRNA and protein levels in VAT and a lower mRNA expression after a high-fat meal in peripheral blood mononuclear cells. The FAs effect on GPR120 mRNA and protein levels in visceral adipocytes was lower in morbidly obese subjects.
authors.affiliation: Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain; CIBERDEM, Instituto de Salud Carlos III, Malaga, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Rodriguez-Pacheco
authors.forename: Sara
authors.initials: S
authors.lastname: Garcia-Serrano
authors.forename: Eva
authors.initials: E
authors.lastname: Garcia-Escobar
authors.forename: Carolina
authors.initials: C
authors.lastname: Gutierrez-Repiso
authors.forename: Juan
authors.initials: J
authors.lastname: Garcia-Arnes
authors.forename: Sergio
authors.initials: S
authors.lastname: Valdes
authors.forename: Montserrat
authors.initials: M
authors.lastname: Gonzalo
authors.forename: Federico
authors.initials: F
authors.lastname: Soriguer
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Moreno-Ruiz
authors.forename: Alberto
authors.initials: A
authors.lastname: Rodriguez-Cañete
authors.forename: Jose L
authors.initials: JL
authors.lastname: Gallego-Perales
authors.forename: Abelardo
authors.initials: A
authors.lastname: Martinez-Ferriz
authors.forename: Gemma
authors.initials: G
authors.lastname: Rojo-Martínez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Garcia-Fuentes
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000328:Adult; D016022:Case-Control Studies; D059305:Diet, High-Fat / Q000009:adverse effects; D005227:Fatty Acids / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009767:Obesity, Morbid / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D012016:Reference Values
chemical_list: C519853:FFAR4 protein, human; D005227:Fatty Acids; D043562:Receptors, G-Protein-Coupled
keywords: Adipocyte; Adipose tissue; Fatty acids; GPR120; High-fat meal