title: Application of ΦSP-1 and ΦSP-3 as a therapeutic strategy against Salmonella Enteritidis infection using Caenorhabditis elegans as model organism.
abstract: The potential of Salmonella-specific phages ΦSP-1 and ΦSP-3 as biocontrol agents was studied in vitro, employing host cell lysis test and in vivo, using Caenorhabditis elegans as a model organism. For in vivo testing, stage 4 C. elegans larvae were experimentally infected with the pathogen Salmonella. Worm mortality was scored for 10 days. TD50 (the time required for 50% of the nematodes to die) of infected worms in the presence of bacteriophages was comparable to uninfected worms, and the two phages provided an increased protection than each one. This study in addition demonstrated the simplicity, elegance, and the cost effectiveness of the C. elegans model for in vivo validation.
authors.affiliation: Department of Biotechnology, Cochin University of Science and Technology, Cochin, India.
authors.forename: Jeena
authors.initials: J
authors.lastname: Augustine
authors.forename: Mridula V
authors.initials: MV
authors.lastname: Gopalakrishnan
authors.forename: Sarita G
authors.initials: SG
authors.lastname: Bhat
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000502:physiology*; D017173:Caenorhabditis elegans / Q000382:microbiology; D006801:Humans; D050296:Microbial Viability; D012480:Salmonella Infections / Q000628:therapy*; D012477:Salmonella enteritidis / Q000821:virology*
keywords: Enteritidis; antibiotic resistance; biocontrol agent; lytic phage