title: Consumption of cranberry beverage improved endogenous antioxidant status and protected against bacteria adhesion in healthy humans: a randomized controlled trial.
abstract: Consumption of polyphenol-rich foods is associated with lower risk from many chronic diseases. We hypothesized that a single dose of cranberry beverage would improve indices of oxidative stress, inflammation, and urinary antibacterial adhesion activity in healthy humans. Six males and 6 females (18-35 years; body mass index, 19-25 kg/m(2)) consumed placebo, cranberry leaf extract beverage, or low-calorie cranberry juice cocktail (LCJC) once in a randomized, double-blind, placebo-controlled cross-over experimental design trial. The washout period between beverages was 1 week. Blood was collected 0, 2, 4, 8, and 24 hours after beverage consumption for measuring oxidative and inflammatory biomarkers. Urine was collected at 0, 0 to 3, 3 to 6, 6 to 9, 9 to 12, and 24 hours postintervention to assess antibacterial adhesion activity. Consumption of cranberry leaf extract beverage elevated (P < .05) blood glutathione peroxidase activity, whereas LCJC consumption increased (P < .05) glutathione concentrations and superoxide dismutase activity compared with placebo. Cranberry leaf extract beverage and LCJC consumption had no effect on the inflammatory biomarkers measured as compared with placebo. At 0 to 3 hours postconsumption, urine from participants who consumed cranberry beverages had higher (P < .05) ex vivo antiadhesion activity against P-fimbriated Escherichia coli compared with placebo. An acute dose of cranberry beverages improved biomarkers of antioxidant status and inhibition of bacterial adhesion in urine.
authors.affiliation: School of Food Science, Washington State University, Pullman, WA.
authors.forename: Bridget D
authors.initials: BD
authors.lastname: Mathison
authors.affiliation: School of Food Science, Washington State University, Pullman, WA.
authors.forename: Lindsey L
authors.initials: LL
authors.lastname: Kimble
authors.affiliation: Ocean Spray Cranberries, Inc, Middleboro, MA.
authors.forename: Kerrie L
authors.initials: KL
authors.lastname: Kaspar
authors.affiliation: Ocean Spray Cranberries, Inc, Middleboro, MA.
authors.forename: Christina
authors.initials: C
authors.lastname: Khoo
authors.affiliation: School of Food Science, Washington State University, Pullman, WA. Electronic address: boonchew@wsu.edu.
authors.forename: Boon P
authors.initials: BP
authors.lastname: Chew
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D001628:Beverages; D018592:Cross-Over Studies; D004311:Double-Blind Method; D004926:Escherichia coli; D005260:Female; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D018515:Plant Leaves; D028321:Plant Preparations / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology; D012016:Reference Values; D013482:Superoxide Dismutase / Q000097:blood; D029799:Vaccinium macrocarpon / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D028321:Plant Preparations; D059808:Polyphenols; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Bacterial adhesion; Humans; Inflammation; Oxidative stress; Vaccinium macrocarpon