title: Pseudorabies viral replication is inhibited by a novel target of miR-21.
abstract: The pseudorabies virus (PRV) is a porcine virus classified as a member of the Alphaherpesvirinae subfamily of Herpesviridae. Recent studies have confirmed that viruses regulate the gene expression in host cells. Commonly affected genes include oxidative-stress response genes, genes involved in the phosphatidylinositol 3-kinase/Protein Kinase B (PI3K/Akt) signaling pathway, and interferon- and interleukin-related genes. However, the post-transcriptional regulation of host genes following PRV infection is hitherto unclear. In this study, we used miRNA microarray approaches to assess miRNA expression in PRV-infected porcine kidney 15 cell line (PK-15), and observed that miR-21 was expressed at high level 12h after the cells were infected with PRV. Furthermore, we identified chemokine (C-X-C motif) ligand 10 (CXCL10), also named interferon-γ inducible protein-10 (IP-10), as a novel target gene of miR-21. IP-10 was down-regulated at 4h after PRV infection. PRV replication was significantly inhibited by IP-10 overexpression.
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Institute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Science, Hangzhou 310021, Zhejiang, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, PR China.
authors.forename: Guojian
authors.initials: G
authors.lastname: Ma
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Fu
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jia
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Mengjin
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Xinyun
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China. Electronic address: shzhao@mail.hzau.edu.cn.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002460:Cell Line; D054357:Chemokine CXCL10 / Q000037:antagonists & inhibitors* / Q000235:genetics; D020869:Gene Expression Profiling; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology* / Q000502:physiology*; D054884:Host-Pathogen Interactions; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D046228:Microarray Analysis; D013552:Swine; D014779:Virus Replication
chemical_list: D054357:Chemokine CXCL10; D035683:MicroRNAs
keywords: CXCL10; PK-15; Pseudorabies virus; Virus replication; miR-21