title: PIK3CA and TP53 gene mutations in human breast cancer tumors frequently detected by ion torrent DNA sequencing.
abstract: Breast cancer is the most common malignancy and the leading cause of cancer deaths in women worldwide. While specific genetic mutations have been linked to 5-10% of breast cancer cases, other environmental and epigenetic factors influence the development and progression of the cancer. Since unique mutations patterns have been observed in individual cancer samples, identification and characterization of the distinctive breast cancer molecular profile is needed to develop more effective target therapies. Until recently, identifying genetic cancer mutations via personalized DNA sequencing was impractical and expensive. The recent technological advancements in next-generation DNA sequencing, such as the semiconductor-based Ion Torrent sequencing platform, has made DNA sequencing cost and time effective with more reliable results. Using the Ion Torrent Ampliseq Cancer Panel, we sequenced 737 loci from 45 cancer-related genes to identify genetic mutations in 105 human breast cancer samples. The sequencing analysis revealed missense mutations in PIK3CA, and TP53 genes in the breast cancer samples of various histologic types. Thus, this study demonstrates the necessity of sequencing individual human cancers in order to develop personalized drugs or combination therapies to effectively target individual, breast cancer-specific mutations.
authors.affiliation: Central Laboratory, People's Hospital of Shan Xi Province, Xian, China.
authors.forename: Xusheng
authors.initials: X
authors.lastname: Bai
authors.affiliation: Central Laboratory, People's Hospital of Shan Xi Province, Xian, China.
authors.forename: Enke
authors.initials: E
authors.lastname: Zhang
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Ye
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
authors.forename: Vijayalakshmi
authors.initials: V
authors.lastname: Nandakumar
authors.affiliation: Central Laboratory, People's Hospital of Shan Xi Province, Xian, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Central Laboratory, People's Hospital of Shan Xi Province, Xian, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Chen
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Chuanning
authors.initials: C
authors.lastname: Tang
authors.affiliation: Central Laboratory, People's Hospital of Shan Xi Province, Xian, China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Li
authors.affiliation: Central Laboratory, People's Hospital of Shan Xi Province, Xian, China.
authors.forename: Huijin
authors.initials: H
authors.lastname: Li
authors.affiliation: Central Laboratory, People's Hospital of Shan Xi Province, Xian, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Central Laboratory, People's Hospital of Shan Xi Province, Xian, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lou
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Dong
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Guangchun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Zhishou
authors.initials: Z
authors.lastname: Dong
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Baishuai
authors.initials: B
authors.lastname: Guo
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: He
authors.initials: H
authors.lastname: Yan
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Chaowei
authors.initials: C
authors.lastname: Yan
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Su
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
authors.forename: Lindsey
authors.initials: L
authors.lastname: Jones
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
authors.forename: Xue F
authors.initials: XF
authors.lastname: Huang
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
authors.forename: Si-Yi
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Central Laboratory, People's Hospital of Shan Xi Province, Xian, China.
authors.forename: Jinglong
authors.initials: J
authors.lastname: Gao
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D058534:Class I Phosphatidylinositol 3-Kinases; D005091:Exons; D005260:Female; D016158:Genes, p53; D006801:Humans; D020125:Mutation, Missense; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human