title: Shiga-toxin genes and genetic diversity of Escherichia coli isolated from pasteurized cow milk in Brazil.
abstract: This study evaluated the genetic similarity and prevalence of the stx1, stx2, eae, and ehxA genes in Escherichia coli isolated from pasteurized cow milk. Eighty-seven E. coli isolates from pasteurized cow milk from 22 dairies located in northwestern Paraná state, Brazil, were analyzed. Genetic similarity was evaluated using enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (ERIC-PCR) and repetitive extragenic palindromic sequence PCR (REP-PCR). E. coli isolates were also analyzed by PCR to investigate the presence of the stx1, stx2, eae, and ehxA genes. ERIC-PCR and REP-PCR clustered 87 bacterial isolates in 76 and 81 genomic profiles, respectively. Both techniques revealed high genetic diversity among the E. coli isolates, confirming the possibility of their use in epidemiological studies. The stx1, stx2, eae, and ehxA virulence genes were not detected in E. coli isolates, indicating a low prevalence of Shiga toxin-producing E. coli in milk produced in the region studied.
  
  
  CONCLUSIONS
  Knowledge about the presence of diarrheagenic Escherichia coli in pasteurized milk is important developing and implementing control measures in milk and dairy production.
authors.affiliation: Graduate Program in Food Sciences, Center of Agrarian Sciences, State Univ. of Maringá, Colombo Ave. 5790, Block J-45, Maringá, Paraná, 87020-900, Brazil.
authors.forename: Simone Aparecida
authors.initials: SA
authors.lastname: Hoffmann
authors.forename: Gabriella Giani
authors.initials: GG
authors.lastname: Pieretti
authors.forename: Adriana
authors.initials: A
authors.lastname: Fiorini
authors.forename: Eliana Valéria
authors.initials: EV
authors.lastname: Patussi
authors.forename: Rosilene Fressatti
authors.initials: RF
authors.lastname: Cardoso
authors.forename: Jane Martha Graton
authors.initials: JM
authors.lastname: Mikcha
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D001938:Brazil; D002417:Cattle; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000517:prevention & control; D029968:Escherichia coli Proteins / Q000235:genetics; D005260:Female; D005516:Food Microbiology; D005798:Genes, Bacterial; D014644:Genetic Variation; D006801:Humans; D008892:Milk / Q000382:microbiology*; D061352:Pasteurization; D016133:Polymerase Chain Reaction / Q000379:methods; D022621:Shiga Toxin / Q000235:genetics*; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D037521:Virulence Factors; C073819:eaeA protein, E coli; D022621:Shiga Toxin
keywords: PCR (polymerase chain reaction); Shiga toxin-producing Escherichia coli (STEC); enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (ERIC-PCR); repetitive extragenic palindromic sequence polymerase chain reaction (REP-PCR); virulence genes