title: Chromatin structure and replication origins: determinants of chromosome replication and nuclear organization.
abstract: The DNA replication program is, in part, determined by the epigenetic landscape that governs local chromosome architecture and directs chromosome duplication. Replication must coordinate with other biochemical processes occurring concomitantly on chromatin, such as transcription and remodeling, to insure accurate duplication of both genetic and epigenetic features and to preserve genomic stability. The importance of genome architecture and chromatin looping in coordinating cellular processes on chromatin is illustrated by two recent sets of discoveries. First, chromatin-associated proteins that are not part of the core replication machinery were shown to affect the timing of DNA replication. These chromatin-associated proteins could be working in concert, or perhaps in competition, with the transcriptional machinery and with chromatin modifiers to determine the spatial and temporal organization of replication initiation events. Second, epigenetic interactions are mediated by DNA sequences that determine chromosomal replication. In this review, we summarize recent findings and current models linking spatial and temporal regulation of the replication program with epigenetic signaling. We discuss these issues in the context of the genome's three-dimensional structure with an emphasis on events occurring during the initiation of DNA replication.
authors.affiliation: DNA Replication Group, Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA.
authors.forename: Owen K
authors.initials: OK
authors.lastname: Smith
authors.affiliation: DNA Replication Group, Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA. Electronic address: aladjemm@mail.nih.gov.
authors.forename: Mirit I
authors.initials: MI
authors.lastname: Aladjem
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics*; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication / Q000235:genetics*; D042822:Genomic Instability / Q000235:genetics; D006801:Humans; D008957:Models, Genetic; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D018741:Replication Origin / Q000235:genetics*
chemical_list: D002843:Chromatin; D009687:Nuclear Proteins
keywords: DNA replication; cell cycle; chromatin organization