title: Fructosamine 3-kinase and glyoxalase I polymorphisms and their association with soluble RAGE and adhesion molecules in diabetes.
abstract: Advanced glycation end-products (AGEs) are key players in pathogenesis of long-term vascular diabetes complications. Several enzymes such as fructosamine 3-kinase (FN3K) and glyoxalase I (GLO I) are crucial in preventing glycation processes. The aim of our study was to evaluate an association of FN3K (rs1056534, rs3848403) and GLO1 rs4746 polymorphisms with parameters of endothelial dysfunction and soluble receptor for AGEs (sRAGE) in 595 diabetic and non-diabetic subjects. Genotypic and allelic frequencies of mentioned polymorphisms did not differ between subgroups. In diabetic patients significant differences were observed in sRAGE concentrations according to their rs1056534 and rs3848403 genotype. While GG and CG genotypes of rs1056534 with mutated G allele were associated with significant decrease of sRAGE (GG: 1055+/-458 and CG: 983+/-363 vs. CC: 1796+/-987 ng/l, p<0.0001), in rs3848403 polymorphism TT genotype with mutated T allele was related with significant sRAGE increase (TT: 1365+/-852 vs. CT: 1016+/-401 and CC: 1087+/-508 ng/l, p=0.05). Significant differences in adhesion molecules were observed in genotype subgroups of GLO1 rs4746 polymorphism. In conclusion, this is the first study describing significant relationship of FN3K (rs1056534) and (rs3848403) polymorphisms with concentration of sRAGE in patients with diabetes.
authors.affiliation: Third Department of Internal Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital, Prague, Czech Republic. jan.skrha@seznam.cz.
authors.forename: J
authors.initials: J
authors.lastname: Škrha
authors.forename: A
authors.initials: A
authors.lastname: Muravská
authors.forename: M
authors.initials: M
authors.lastname: Flekač
authors.forename: E
authors.initials: E
authors.lastname: Horová
authors.forename: J
authors.initials: J
authors.lastname: Novák
authors.forename: A
authors.initials: A
authors.lastname: Novotný
authors.forename: M
authors.initials: M
authors.lastname: Prázný
authors.forename: J
authors.initials: J
authors.lastname: Škrha
authors.forename: J
authors.initials: J
authors.lastname: Kvasnička
authors.forename: L
authors.initials: L
authors.lastname: Landová
authors.forename: M
authors.initials: M
authors.lastname: Jáchymová
authors.forename: T
authors.initials: T
authors.lastname: Zima
authors.forename: M
authors.initials: M
authors.lastname: Kalousová
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000175:diagnosis / Q000201:enzymology* / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000175:diagnosis / Q000201:enzymology* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D011110:Polymorphism, Genetic; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000097:blood*; D012307:Risk Factors; D055815:Young Adult
chemical_list: D015415:Biomarkers; D015815:Cell Adhesion Molecules; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D017853:Phosphotransferases (Alcohol Group Acceptor); C414347:fructosamine-3-kinase; D007791:Lactoylglutathione Lyase