title: Testing Aβ toxicity on primary CNS cultures using drug-screening microfluidic chips.
abstract: Open microscale cultures of primary central nervous system (CNS) cells have been implemented in microfluidic chips that can expose the cells to physiological fluidic shear stress conditions. Cells in the chips were exposed to differently aggregated forms of beta-amyloid (Aβ), i.e. conditions mimicking an Alzheimer's Disease environment, and treated with CNS drugs in order to assess the contribution of glial cells during pharmacological treatments. FTY720, a drug approved for the treatment of Multiple Sclerosis, was found to play a marked neuroprotective role in neuronal cultures as well as in microglia-enriched neuronal cultures, preventing neurodegeneration after cell exposure to neurotoxic oligomers of Aβ.
authors.affiliation: Department of Medical Biotechnologies and Translational Medicine, University of Milan, Via L. Vanvitelli 32, 20129 Milan, Italy. aruizmo@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Ruiz
authors.forename: P
authors.initials: P
authors.lastname: Joshi
authors.forename: R
authors.initials: R
authors.lastname: Mastrangelo
authors.forename: M
authors.initials: M
authors.lastname: Francolini
authors.forename: C
authors.initials: C
authors.lastname: Verderio
authors.forename: M
authors.initials: M
authors.lastname: Matteoli
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004622:Embryo, Mammalian / Q000166:cytology; D004867:Equipment Design; D000068876:Fingolimod Hydrochloride; D057166:High-Throughput Screening Assays / Q000295:instrumentation*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D017628:Microglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D018697:Nootropic Agents / Q000494:pharmacology / Q000627:therapeutic use; D010446:Peptide Fragments / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D066330:Printing, Three-Dimensional; D011409:Propylene Glycols / Q000494:pharmacology / Q000627:therapeutic use; D066263:Protein Aggregation, Pathological / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D033081:Shear Strength; D013110:Sphingosine / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D013499:Surface Properties
chemical_list: D016229:Amyloid beta-Peptides; D004129:Dimethylpolysiloxanes; D018696:Neuroprotective Agents; D018697:Nootropic Agents; D010446:Peptide Fragments; D011409:Propylene Glycols; C075222:amyloid beta-protein (1-42); C013830:baysilon; D000068876:Fingolimod Hydrochloride; D013110:Sphingosine