title: DUF581 is plant specific FCS-like zinc finger involved in protein-protein interaction.
abstract: Zinc fingers are a ubiquitous class of protein domain with considerable variation in structure and function. Zf-FCS is a highly diverged group of C2-C2 zinc finger which is present in animals, prokaryotes and viruses, but not in plants. In this study we identified that a plant specific domain of unknown function, DUF581 is a zf-FCS type zinc finger. Based on HMM-HMM comparison and signature motif similarity we named this domain as FCS-Like Zinc finger (FLZ) domain. A genome wide survey identified that FLZ domain containing genes are bryophytic in origin and this gene family is expanded in spermatophytes. Expression analysis of selected FLZ gene family members of A. thaliana identified an overlapping expression pattern suggesting a possible redundancy in their function. Unlike the zf-FCS domain, the FLZ domain found to be highly conserved in sequence and structure. Using a combination of bioinformatic and protein-protein interaction tools, we identified that FLZ domain is involved in protein-protein interaction.
authors.affiliation: National Institute of Plant Genome Research, New Delhi, India.
authors.forename: Muhammed Jamsheer
authors.initials: MJ
authors.lastname: K
authors.affiliation: National Institute of Plant Genome Research, New Delhi, India.
authors.forename: Ashverya
authors.initials: A
authors.lastname: Laxmi
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism; D019295:Computational Biology; D030562:Databases, Protein; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D025941:Protein Interaction Mapping; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers
chemical_list: D010940:Plant Proteins