title: Influence of resistance exercise intensity and metabolic stress on anabolic signaling and expression of myogenic genes in skeletal muscle.
abstract: BACKGROUND
  We investigated the effect of resistance exercise intensity and exercise-induced metabolic stress on the activation of anabolic signaling and expression of myogenic genes in skeletal muscle.
  
  
  METHODS
  Ten strength-trained athletes performed high-intensity [HI, 74% of 1-repetition maximum (RM)], middle-intensity (MI, 54% 1RM), or middle-intensity (54% 1RM) no-relaxation exercise (MIR). Kinase phosphorylation level and myogenic gene expression in muscle samples were evaluated before, 45 min, 5 h, and 20 h after exercise.
  
  
  RESULTS
  The lactate concentration in MI was approximately 2-fold lower than in the 2 other sessions, and was highest in MIR. The phosphorylation level of extracellular kinase 1/2Thr202/Tyr204 after exercise was related to metabolic stress. Metabolic stress induced a decrease in myostatin mRNA expression, whereas mechano-growth factor mRNA level depended on exercise intensity.
  
  
  CONCLUSIONS
  This study demonstrates that both intensity and exercise-induced metabolic stress can be manipulated to affect muscle anabolic signaling.
authors.affiliation: Laboratory of Exercise Physiology, Institute of Biomedical Problems, Russian Academy of Sciences, 76A Khoroshevskoe Shosse, Moscow, 123007, Russia.
authors.forename: Daniil V
authors.initials: DV
authors.lastname: Popov
authors.forename: Evgeny A
authors.initials: EA
authors.lastname: Lysenko
authors.forename: Anton V
authors.initials: AV
authors.lastname: Bachinin
authors.forename: Tatiana F
authors.initials: TF
authors.lastname: Miller
authors.forename: Nadezda S
authors.initials: NS
authors.lastname: Kurochkina
authors.forename: Irina V
authors.initials: IV
authors.lastname: Kravchenko
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Furalyov
authors.forename: Olga L
authors.initials: OL
authors.lastname: Vinogradova
mesh_terms: D005786:Gene Expression Regulation; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D055435:Myostatin / Q000096:biosynthesis*; D055070:Resistance Training / Q000379:methods*; D050799:STAT5 Transcription Factor / Q000096:biosynthesis*; D013312:Stress, Physiological / Q000502:physiology*; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis*; D055815:Young Adult
chemical_list: C525911:MSTN protein, human; D055435:Myostatin; D050799:STAT5 Transcription Factor; C494092:STAT5A protein, human; D025521:Tumor Suppressor Proteins
keywords: gene expression; metabolic stress; protein kinase; resistance exercise; skeletal muscle