title: Crystallization and preliminary X-ray diffraction analysis of Hypocrea jecorina Cel7A in two new crystal forms.
abstract: Cel7A (previously known as cellobiohydrolase I) from Hypocrea jecorina was crystallized in two crystalline forms, neither of which have been previously reported. Both forms co-crystallize under the same crystallization conditions. The first crystal form belonged to space group C2, with unit-cell parameters a=152.5, b=44.9, c=57.6 Å, β=101.2°, and diffracted X-rays to 1.5 Å resolution. The second crystal form belonged to space group P6₃22, with unit-cell parameters a=b≃155, c≃138 Å, and diffracted X-rays to 2.5 Å resolution. The crystals were obtained using full-length Cel7A, which consists of a large 434-residue N-terminal catalytic domain capable of cleaving cellulose, a 27-residue flexible linker and a small 36-residue C-terminal carbohydrate-binding module (CBM). However, a preliminary analysis of the electron-density maps suggests that the linker and CBM are disordered in both crystal forms. Complete refinement and structure analysis are currently in progress.
authors.affiliation: Molecular and Structural Biochemistry Department, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Annette M
authors.initials: AM
authors.lastname: Bodenheimer
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Cuneo
authors.affiliation: Molecular and Structural Biochemistry Department, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Swartz
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
authors.forename: Junhong
authors.initials: J
authors.lastname: He
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
authors.forename: Hugh M
authors.initials: HM
authors.lastname: O'Neill
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
authors.forename: Dean A A
authors.initials: DA
authors.lastname: Myles
authors.affiliation: Chemical Science Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
authors.forename: Barbara R
authors.initials: BR
authors.lastname: Evans
authors.affiliation: Molecular and Structural Biochemistry Department, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Flora
authors.initials: F
authors.lastname: Meilleur
mesh_terms: D043366:Cellulose 1,4-beta-Cellobiosidase / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D035901:Hypocrea / Q000737:chemistry*
chemical_list: D043366:Cellulose 1,4-beta-Cellobiosidase
keywords: Cel7A; Hypocrea jecorina