title: Dermal administration of manganese porphyrin by iontophoresis.
abstract: The present study describes a technique for dermal administration of cationic manganese porphyrin (Mn-porphyrin), an antioxidant with superoxide dismutase (SOD) activity, in hairless mouse. In general, the stratum corneum on the surface of the skin represents a barrier to passive diffusion of therapeutic agents by standard dermal administration. The present study investigated whether, dermal administration of Mn-porphyrin solution using iontophoresis, the electrical dermal administration technique, could overcome this barrier. We visually confirmed that Mn-porphyrin had penetrated to the reverse side of the hairless mouse skin after iontophoresis for a short period. With prolonged iontophoresis, the ratio of detectable Mn-porphyrin solution on the reverse side of the hairless mouse skin increased. In the future, this technique could provide an innovative approach for delivery of this antioxidant in intractable disease.
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo, Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo, 192-0397, Japan. Electronic address: fuminoito@spice.ocn.ne.jp.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Ito
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo, Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Imamura
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo, Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Asayama
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo, Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kanamura
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo, Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Kawakami
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques; D007478:Iontophoresis; D008345:Manganese / Q000737:chemistry*; D051379:Mice; D008812:Mice, Hairless; D011166:Porphyrins / Q000737:chemistry*; D012867:Skin / Q000473:pathology; D013053:Spectrophotometry; D013997:Time Factors
chemical_list: D000975:Antioxidants; D011166:Porphyrins; D008345:Manganese
keywords: Dermal administration; Hairless mouse; Iontophoresis; Mn-porphyrin; Permeation test; Superoxide dismutase