title: Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32.
abstract: Acinetobacter calcoaceticus HIRFA32 from wheat rhizosphere produced catecholate type of siderophore with optimum siderophore (ca. 92 % siderophore units) in succinic acid medium without FeSO4 at 28 °C and 24 h of incubation. HPLC purified siderophore appeared as pale yellow crystals with molecular weight [M(+1)] m/z 347.18 estimated by LCMS. The structure elucidated by (1)H NMR, (13)C NMR, HMQC, HMBC, NOESY and decoupling studies, revealed that siderophore composed of 2,3-dihydroxybenzoic acid with hydroxyhistamine and threonine as amino acid subunits. In vitro study demonstrated siderophore mediated mycelium growth inhibition (ca. 46.87 ± 0.5 %) of Fusarium oxysporum. This study accounts to first report on biosynthesis of acinetobactin-like siderophore by the rhizospheric strain of A. calcoaceticus and its significance in inhibition of F. oxysporum.
authors.affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune, 411007 India.
authors.forename: D V
authors.initials: DV
authors.lastname: Maindad
authors.affiliation: Department of Chemistry, University of Pune, Pune, 411007 India.
authors.forename: V M
authors.initials: VM
authors.lastname: Kasture
authors.affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune, 411007 India.
authors.forename: H
authors.initials: H
authors.lastname: Chaudhari
authors.affiliation: Department of Chemistry, University of Pune, Pune, 411007 India.
authors.forename: D D
authors.initials: DD
authors.lastname: Dhavale
authors.affiliation: Department of Microbiology, University of Pune, Pune, 411007 India.
authors.forename: B A
authors.initials: BA
authors.lastname: Chopade
authors.affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune, 411007 India.
authors.forename: D P
authors.initials: DP
authors.lastname: Sachdev
keywords: Acinetobacter calcoaceticus; Acinetobactin-like siderophore; Fusarium oxysporum; NMR; Wheat rhizosphere