title: Osteogenic differentiation and mineralization of human exfoliated deciduous teeth stem cells on modified chitosan scaffold.
abstract: Stem cells from human exfoliated deciduous teeth (SHEDs) have been considered as alternative sources of adult stem cells in tissue engineering because of their potential to differentiate into multiple cell lineages. Strontium has an important function in bone remodeling because it can simulate bone formation and decrease bone resorption. In this study, the effects of strontium phosphate on the osteogenic differentiation of SHEDs were investigated. Strontium phosphate was found to enhance the osteogenic differentiation of SHEDs with up-regulated osteoblast-related gene expression. The proliferation of SHEDs was slightly inhibited by chitosan scaffolds; however, type-I collagen expression, alkaline phosphatase activity, and calcium deposition on chitosan scaffolds containing strontium were significantly enhanced. Furthermore, cells seeded in a 3D scaffold under dynamic culture at an optimal fluid rate might enhance cellular differentiation than static culture in osteoblastic gene expression. This experiment might provide a useful cell resource and dynamic 3D culture for tissue engineering and bone repair.
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan. Electronic address: f10549@ntut.edu.tw.
authors.forename: Wen-Ta
authors.initials: WT
authors.lastname: Su
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
authors.forename: Pai-Shuen
authors.initials: PS
authors.lastname: Wu
authors.affiliation: PhytoHealth Corporation, Maywufa Biopharma Group, Taipei, Taiwan.
authors.forename: Chih-Sheng
authors.initials: CS
authors.lastname: Ko
authors.affiliation: Mackay Memorial Hospital, Taipei, Taiwan.
authors.forename: Te-Yang
authors.initials: TY
authors.lastname: Huang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D024042:Collagen Type I / Q000378:metabolism; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects; D010710:Phosphates / Q000737:chemistry / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013324:Strontium / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014094:Tooth, Deciduous / Q000166:cytology*
chemical_list: D024042:Collagen Type I; D010710:Phosphates; D048271:Chitosan; C052774:strontium phosphate; D000469:Alkaline Phosphatase; D002118:Calcium; D013324:Strontium
keywords: Chitosan scaffold; Dynamic culture; Human exfoliated deciduous teeth stem cells (SHEDs); Osteogenic differentiation; Strontium