title: Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing.
abstract: A triazole linkage is formed in RNA by untemplated strain-promoted or CuAAC chemical ligation of 3'-azide and 5'-cyclooctyne oligonucleotides under denaturing conditions. Reverse transcriptase reads through these artificial linkages with omission of one nucleotide. These surprising results have implications for RNA isolation, amplification, sequencing and a variety of biological applications.
authors.affiliation: School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
authors.forename: Xiong
authors.initials: X
authors.lastname: Chen
authors.forename: Afaf H
authors.initials: AH
authors.lastname: El-Sagheer
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D008040:Genetic Linkage / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D048348:Reverse Transcription / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*; D014230:Triazoles / Q000378:metabolism*
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D014230:Triazoles