title: Protein mixtures of environmentally friendly zein to understand protein-protein interactions through biomaterials synthesis, hemolysis, and their antimicrobial activities.
abstract: Industrially important zein protein has been employed to understand its interactions with two model proteins bovine serum albumin (BSA) and cytochrome c (Cyc,c) following the in vitro synthesis of Au NPs so as to expand its applicability for biological applications. Interactions were studied under the effect of temperature variation by UV-visible and fluorescence emission studies. Temperature induced unfolding in the protein mixtures indicated their degree of mutual interactions through simultaneous nucleation of gold nanoparticles (Au NPs) and their subsequent shape control effects. Zein + BSA mixtures showed favorable protein-protein interactions over the entire mole fraction range with maximum close to x(BSA) = 0.24, whereas zein + Cyc,c showed such interactions only in the zein rich region with significant demixing in the Cyc,c rich region of the mixtures. Both hydrophobic as well as hydrophilic domains in the unfolded states were driving such interactions in the case of zein + BSA mixtures while demixing was the result of the predominant hydrophilic nature of Cyc,c and its self-aggregation behavior in the Cyc,c rich region in contrast to the predominant hydrophobic nature of zein. Zein + BSA mixtures produced small roughly spherical Au NPs fully coated with protein, whereas the demixing zone of zein + Cyc,c mixtures generated highly anisotropic NPs with little protein coating. To explore their biological applications, protein conjugated NPs of both mixtures were subjected to hemolysis where NPs coated with the former mixture showed little hemolysis and may act as drug delivery vehicles in systemic circulation in comparison to the latter. Both kinds of NPs further demonstrated their extraordinary antimicrobial activities with different kinds of strains and proved to be highly important environmentally friendly biomaterials.
authors.affiliation: Department of Chemistry, B.B.K. D.A.V. College for Women, Amritsar 143005, Punjab, India.
authors.forename: Aabroo
authors.initials: A
authors.lastname: Mahal
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Goshisht
authors.forename: Poonam
authors.initials: P
authors.lastname: Khullar
authors.forename: Harsh
authors.initials: H
authors.lastname: Kumar
authors.forename: Narinder
authors.initials: N
authors.lastname: Singh
authors.forename: Gurinder
authors.initials: G
authors.lastname: Kaur
authors.forename: Mandeep Singh
authors.initials: MS
authors.lastname: Bakshi
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D003198:Computer Simulation; D045304:Cytochromes c / Q000737:chemistry*; D004058:Diffusion; D006046:Gold / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects*; D008422:Materials Testing; D008956:Models, Chemical; D008958:Models, Molecular; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D015028:Zein / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D053769:Nanocapsules; D012710:Serum Albumin, Bovine; D006046:Gold; D045304:Cytochromes c; D015028:Zein