title: Regulation of dynamin oligomerization in cells: the role of dynamin-actin interactions and its GTPase activity.
abstract: Dynamin is a 96-kDa protein that has multiple oligomerization states that influence its GTPase activity. A number of different dynamin effectors, including lipids, actin filaments, and SH3-domain-containing proteins, have been implicated in the regulation of dynamin oligomerization, though their roles in influencing dynamin oligomerization have been studied predominantly in vitro using recombinant proteins. Here, we identify higher order dynamin oligomers such as rings and helices in vitro and in live cells using fluorescence lifetime imaging microscopy (FLIM). FLIM detected GTP- and actin-dependent dynamin oligomerization at distinct cellular sites, including the cell membrane and transition zones where cortical actin transitions into stress fibers. Our study identifies a major role for direct dynamin-actin interactions and dynamin's GTPase activity in the regulation of dynamin oligomerization in cells.
authors.affiliation: Division of Nephrology, Massachusetts General Hospital, Charlestown, MA, 02129, USA.
authors.forename: Changkyu
authors.initials: C
authors.lastname: Gu
authors.forename: Joann
authors.initials: J
authors.lastname: Chang
authors.forename: Valentina A
authors.initials: VA
authors.lastname: Shchedrina
authors.forename: Vincent A
authors.initials: VA
authors.lastname: Pham
authors.forename: John H
authors.initials: JH
authors.lastname: Hartwig
authors.forename: Worawit
authors.initials: W
authors.lastname: Suphamungmee
authors.forename: William
authors.initials: W
authors.lastname: Lehman
authors.forename: Bradley T
authors.initials: BT
authors.lastname: Hyman
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Bacskai
authors.forename: Sanja
authors.initials: S
authors.lastname: Sever
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D034281:Dynamins / Q000737:chemistry / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary
chemical_list: D000199:Actins; D006160:Guanosine Triphosphate; D034281:Dynamins
keywords: actin; dynamin; dynamin oligomerization; fluorescence lifetime imaging microscopy