title: Profiling of glucosinolates and flavonoids in Rorippa indica (Linn.) Hiern. (Cruciferae) by UHPLC-PDA-ESI/HRMS(n).
abstract: An UHPLC-PDA-ESI/HRMS(n) profiling method was used to identify the glucosinolates and flavonoids of Rorippa indica (Cruciferae), a wild vegetable and Chinese herb used to treat cough, diarrhea, and rheumatoid arthritis. Thirty-three glucosinolates, more than 40 flavonol glycosides, and 18 other phenolic and common organic compounds were identified. The glucosinolates and polyphenols were separated by UHPLC. High-resolution deprotonated molecules provided high accuracy mass values that were used to determine formulas and provide putative identification of the glucosinolates and flavonoids. The fragments from multistage mass spectrometry were used to elucidate the structures. The concentrations of the main components were based on UV peak areas and molar relative response factors with a single calibration standard. This study found this plant to be a rich source for glucosinolates, containing 24 new glucosinolates, including 14 glucosylated glucosinolates that were previously unidentified.
authors.affiliation: Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Building-161, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705, United States.
authors.forename: Long-Ze
authors.initials: LZ
authors.lastname: Lin
authors.forename: Jianghao
authors.initials: J
authors.lastname: Sun
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
authors.forename: Ren-Wei
authors.initials: RW
authors.lastname: Zhang
authors.forename: Xiao-E
authors.initials: XE
authors.lastname: Fan
authors.forename: Lai-Wei
authors.initials: LW
authors.lastname: Li
authors.forename: James M
authors.initials: JM
authors.lastname: Harnly
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D055664:Electrochemical Techniques; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D055951:Functional Food / Q000032:analysis* / Q000191:economics; D005961:Glucosinolates / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D010783:Photometry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D031225:Rorippa / Q000737:chemistry*; D036226:Seedlings / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D005419:Flavonoids; D005961:Glucosinolates; D010936:Plant Extracts