title: Functional toll-like receptor 3 expressed by oral squamous cell carcinoma induced cell apoptosis and decreased migration.
abstract: OBJECTIVE
  The aim of this study was to investigate the expression and function of toll-like receptor 3 (TLR3) in oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  We first assessed TLR3 expression in 20 cases of primary OSCC tissue. Two OSCC cell lines, SCC4 and CAL27, were used for further study. Lyophilized polyinosinic-polycytidylic acid [Poly(I:C)] was used to activate TLR3 expressed by OSCC. Changes in cytokines expression, cell viability, apoptosis, and migration in OSCC were investigated.
  
  
  RESULTS
  TLR3 was present in both OSCC tissue and the 2 OSCC cell lines examined. Poly(I:C) stimulated robust responses in OSCC: it upregulated cytokine expression; decreased cell viability by suppressing cell proliferation and inducing apoptosis; and decreased cell migration. Poly(I:C)-TLR3-induced OSCC cell apoptosis was caspase-3-dependent.
  
  
  CONCLUSIONS
  The present study indicated that TLR3 might affect OSCC development and should be considered as a potential target for future OSCC immunotherapy.
authors.affiliation: Central Laboratory of Stomatology, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: He
authors.affiliation: Central Laboratory of Stomatology, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Huang
authors.affiliation: Central Laboratory of Stomatology, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, China.
authors.forename: Peihua
authors.initials: P
authors.lastname: Shi
authors.affiliation: Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, China. Electronic address: Qghu@nju.edu.cn.
authors.forename: Qingang
authors.initials: Q
authors.lastname: Hu
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051196:Toll-Like Receptor 3 / Q000378:metabolism*
chemical_list: D016207:Cytokines; C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3