title: Characterization of the effect of LPS on dendritic cell subset discrimination in spleen.
abstract: The Gram-negative bacterial endotoxin lipopolysaccharide (LPS) is a potent inflammatory mediator and a leading cause of bacterial sepsis. While LPS is known to activate antigen-presenting cells, here we find that LPS down-regulates expression of CD11c and CD11b on splenic dendritic cell subsets, thus confounding the ability to identify these subsets following treatment. This has implications with regard to tracking the response to LPS in terms of the cell subsets involved, and should be considered whenever such studies are undertaken.
authors.affiliation: Research School of Biology, The Australian National University, Canberra, ACT, Australia.
authors.forename: Kristin L
authors.initials: KL
authors.lastname: Griffiths
authors.forename: Jonathan K H
authors.initials: JK
authors.lastname: Tan
authors.forename: Helen C
authors.initials: HC
authors.lastname: O'Neill
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D039521:CD11c Antigen / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D015536:Down-Regulation; D004705:Endocytosis; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology*
chemical_list: D039481:CD11b Antigen; D039521:CD11c Antigen; D008070:Lipopolysaccharides
keywords: dendritic cell; lipopolysaccharide; spleen