title: Deletion polymorphism of GSTT1 gene as protective marker for allergic rhinitis.
abstract: OBJECTIVE
  Allergic rhinitis (AR) is one of the most common respiratory diseases among human populations. Strong evidence suggests that genetic predisposition and environmental factors could contribute to the development of this complex disease. Glutathione S-transferases (GSTs) are biomarkers of inflammation and oxidative stress. These phase II enzymes play a significant role in detoxifying xenobiotic compounds. To analyze the role of GST gene polymorphisms in AR pathogenesis in a case-control population of 103 patients affected by AR and 200 healthy non-allergic subjects.
  
  
  METHODS
  We screened genomic DNA extracted from buccal cells for GSTM1 positive/null, GSTP1*I105V (rs1695) and GSTT1 positive/null polymorphisms. The X(2) -test, odds ratio (OR) and logistic regression were used as statistical analyses.
  
  
  RESULTS
  Significant differences in null genotype distribution between AR patients (13%) and healthy controls (30%) were found for the GSTT1 null genotype (OR = 0.30, 95% confidence interval = 0.14-0.65; P = 0.001). GSTM1 and GSTP1 polymorphisms did not show any significant results.
  
  
  CONCLUSIONS
  Our data indicated that GSTT1 may be a susceptibility locus for AR. Specifically, the positive/null polymorphism of GSTT1 may be involved in an oxidative stress-related mechanism that may enhance pathogenic pathways related to AR. Moreover, beside GSTT1, this deletion polymorphism affects also another gene potentially related to AR phenotype, LOC391322. This gene belongs to MIF (macrophage migration inhibitory factor) gene family and several studies indicated the role of this gene in several immunology-related phenotypes. Therefore, two different scenarios may explain the observed genetic association.
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.|Clinical Pathophysiology Center, AFaR - 'San Giovanni Calibita' Fatebenefratelli Hospital, Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Iorio
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Polimanti
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Piacentini
authors.affiliation: Clinical Pathology Department, AFaR - 'San Giovanni Calibita' Fatebenefratelli Hospital, Rome, Italy.
authors.forename: Giancarlo Maria
authors.initials: GM
authors.lastname: Liumbruno
authors.affiliation: Clinical Pathophysiology Center, AFaR - 'San Giovanni Calibita' Fatebenefratelli Hospital, Rome, Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: Manfellotto
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Fuciarelli
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002892:Chromosomes, Human, Pair 22; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D065631:Rhinitis, Allergic / Q000235:genetics*; D017384:Sequence Deletion
chemical_list: D005819:Genetic Markers; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase
keywords: allergy; association study; genetic predisposition; genetic variants; glutathione S-transferases; respiratory diseases