title: Effect of leptin and apelin preconditioning on hepatic ischemia reperfusion injury in rats.
abstract: Leptin and apelin are important adipocytokines involved in a variety of endocrine and paracrine functions. The aim of this study was to evaluate the effect of exogenous leptin and apelin preconditioning on hepatic ischemia reperfusion (I/R) injury in rats. Forty mice were assigned to four groups (n = 10): sham-operated control (sham), I/R injury, I/R + leptin (I/R + L), and I/R + apelin (I/R + A). Leptin 100 μg/kg/day and apelin 2 μg/kg/day were delivered intraperitoneally starting 3 days prior to surgical procedure in I/R + L and I/R + A groups, respectively. All I/R groups underwent 45 min of warm ischemia, followed by 30 min of reperfusion. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), liver malondialdehyde (MDA) and glutathione (GSH), and liver histopathology were compared between groups. MDA was elevated in I/R, but stayed similar in I/R + L and I/R + A compared to sham. I/R + A had significantly lower MDA compared to I/R. GSH levels did not differ significantly between the groups. ALT and AST were elevated in all I/R groups, but significant reduction was observed in I/R + L and I/R + A compared to I/R. Liver histopathology was mostly mild in I/R + L and I/R + A, in contrast to severe injury observed in the I/R group. Leptin and apelin preconditioning significantly reduced hepatic I/R injury in rats.
authors.affiliation: Faculty of Medicine Department of General Surgery, Trakya University, Trakya Üniversitesi Tıp Fakültesi Genel Cerrahi, AD 22030 Edirne, Turkey.
authors.forename: Tamer
authors.initials: T
authors.lastname: Sagiroglu
authors.affiliation: Faculty of Medicine Department of Emergency Service, Trakya University, Trakya Üniversitesi Tıp Fakültesi Acil Servis, AD 22030 Edirne, Turkey.
authors.forename: Mustafa Burak
authors.initials: MB
authors.lastname: Aksoy
authors.affiliation: Faculty of Medicine Department of Anesthesiology and Reanimation, Trakya University, TrakyaUniversities Tıp Fakültesi Genel Cerrahi, AD 22030 Edirne, Turkey.
authors.forename: Gonul
authors.initials: G
authors.lastname: Sagiroglu
authors.affiliation: Faculty of Medicine Department of Medical Genetics, Trakya University, Trakya Üniversitesi Tıp Fakültesi Tıbbi Biyoloji ve Genetik, AD 22100 Edirne, Turkey.
authors.forename: Hilmi
authors.initials: H
authors.lastname: Tozkir
authors.affiliation: Faculty of Medicine Department of General Surgery, Trakya University, Trakya Üniversitesi Tıp Fakültesi Genel Cerrahi, AD 22030 Edirne, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Oguz
authors.affiliation: Faculty of Medicine Department of Pathology, Trakya University, Trakya Üniversitesi Tıp FakültesiPatoloji, AD 22030 Edirne, Turkey.
authors.forename: Tulin
authors.initials: T
authors.lastname: Yalta
authors.affiliation: Hakkari State Hospital, Hakkari Devlet Hastanesi, Hakkari, Turkey.
authors.forename: Mehmet A
authors.initials: MA
authors.lastname: Yagci
authors.affiliation: Faculty of Medicine Department of General Surgery, Trakya University, Trakya Üniversitesi Tıp Fakültesi Genel Cerrahi, AD 22030 Edirne, Turkey.
authors.forename: Atakan
authors.initials: A
authors.lastname: Sezer
keywords: Apelin; Ischemia reperfusion injury; Leptin; Liver