title: Developmental validation of the PowerPlex(®) Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards.
abstract: The original CODIS database based on 13 core STR loci has been overwhelmingly successful for matching suspects with evidence. Yet there remain situations that argue for inclusion of more loci and increased discrimination. The PowerPlex(®) Fusion System allows simultaneous amplification of the following loci: Amelogenin, D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, DYS391, D8S1179, D12S391, D19S433, FGA, and D22S1045. The comprehensive list of loci amplified by the system generates a profile compatible with databases based on either the expanded CODIS or European Standard Set (ESS) requirements. Developmental validation testing followed SWGDAM guidelines and demonstrated the quality and robustness of the PowerPlex(®) Fusion System across a number of variables. Consistent and high-quality results were compiled using data from 12 separate forensic and research laboratories. The results verify that the PowerPlex(®) Fusion System is a robust and reliable STR-typing multiplex suitable for human identification.
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: katie.oostdik@promega.com.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Oostdik
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: kristy.lenz@promega.com.
authors.forename: Kristy
authors.initials: K
authors.lastname: Lenz
authors.affiliation: Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: NyeJ1@michigan.gov.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Nye
authors.affiliation: Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: schellingk@michigan.gov.
authors.forename: Kristin
authors.initials: K
authors.lastname: Schelling
authors.affiliation: Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: yetd@michigan.gov.
authors.forename: Donald
authors.initials: D
authors.lastname: Yet
authors.affiliation: Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: bruskis@michigan.gov.
authors.forename: Scott
authors.initials: S
authors.lastname: Bruski
authors.affiliation: Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: strongj@michigan.gov.
authors.forename: Joshua
authors.initials: J
authors.lastname: Strong
authors.affiliation: United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: clint.d.buchanan.civ@mail.mil.
authors.forename: Clint
authors.initials: C
authors.lastname: Buchanan
authors.affiliation: United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: joel.d.sutton2.civ@mail.mil.
authors.forename: Joel
authors.initials: J
authors.lastname: Sutton
authors.affiliation: United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: jessica.k.linner.civ@mail.mil.
authors.forename: Jessica
authors.initials: J
authors.lastname: Linner
authors.affiliation: United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: nicole.r.frazier.civ@mail.mil.
authors.forename: Nicole
authors.initials: N
authors.lastname: Frazier
authors.affiliation: Arkansas State Crime Laboratory, 3 Natural Resources Drive, Little Rock, AR 72205, USA. Electronic address: hays.young@crimelab.arkansas.gov.
authors.forename: Hays
authors.initials: H
authors.lastname: Young
authors.affiliation: Los Angeles County Sheriff's Department, 1800 Paseo Rancho Castilla, Los Angeles, CA 90032, USA. Electronic address: LKMatthi@lasd.org.
authors.forename: Learden
authors.initials: L
authors.lastname: Matthies
authors.affiliation: Los Angeles County Sheriff's Department, 1800 Paseo Rancho Castilla, Los Angeles, CA 90032, USA. Electronic address: ARSage@lasd.org.
authors.forename: Amber
authors.initials: A
authors.lastname: Sage
authors.affiliation: Kansas Bureau of Investigation, Forensic Laboratory Division, 1620 Southwest Tyler Street, Topeka, KS 66612, USA. Electronic address: jeff.hahn@kbi.state.ks.us.
authors.forename: Jeff
authors.initials: J
authors.lastname: Hahn
authors.affiliation: Kentucky State Police, 100 Sower Boulevard, Frankfort, KY 40601, USA. Electronic address: regina.wells@ky.gov.
authors.forename: Regina
authors.initials: R
authors.lastname: Wells
authors.affiliation: Washington State Patrol, Crime Laboratory Division, 2203 Airport Way South, Seattle, WA 98134-2028, USA. Electronic address: Natasha.williams@wsp.wa.gov.
authors.forename: Natasha
authors.initials: N
authors.lastname: Williams
authors.affiliation: Washington State Patrol, Crime Laboratory Division, 2203 Airport Way South, Seattle, WA 98134-2028, USA. Electronic address: monica.price@wsp.wa.gov.
authors.forename: Monica
authors.initials: M
authors.lastname: Price
authors.affiliation: Texas Department of Public Safety, Crime Laboratory, 5805 North Lamar Boulevard, Austin, TX 78752, USA. Electronic address: jody.koehler@dps.texas.gov.
authors.forename: Jody
authors.initials: J
authors.lastname: Koehler
authors.affiliation: New Hampshire State Police, Forensic Laboratory, 33 Hazen Drive, Concord, NH 03305, USA. Electronic address: melisa.staples@dos.nh.gov.
authors.forename: Melisa
authors.initials: M
authors.lastname: Staples
authors.affiliation: New Hampshire State Police, Forensic Laboratory, 33 Hazen Drive, Concord, NH 03305, USA. Electronic address: katie.swango@dos.nh.gov.
authors.forename: Katie L
authors.initials: KL
authors.lastname: Swango
authors.affiliation: National Institute of Standards and Technology, Biochemical Science Division, 100 Bureau Drive, Gaithersburg, MA 20899, USA. Electronic address: becky.hill@nist.gov.
authors.forename: Carolyn
authors.initials: C
authors.lastname: Hill
authors.affiliation: Oklahoma State Bureau of Investigation, 800 East 2nd Street, Edmond, OK 73034, USA. Electronic address: karen.oyerly@osbi.ok.gov.
authors.forename: Karen
authors.initials: K
authors.lastname: Oyerly
authors.affiliation: Oklahoma State Bureau of Investigation, 800 East 2nd Street, Edmond, OK 73034, USA. Electronic address: wendy.duke@osbi.ok.gov.
authors.forename: Wendy
authors.initials: W
authors.lastname: Duke
authors.affiliation: Oklahoma State Bureau of Investigation, 800 East 2nd Street, Edmond, OK 73034, USA. Electronic address: Lesley.katzilierakis@osbi.ok.gov.
authors.forename: Lesley
authors.initials: L
authors.lastname: Katzilierakis
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: martin.ensenberger@promega.com.
authors.forename: Martin G
authors.initials: MG
authors.lastname: Ensenberger
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: jeanne.bourdeau@promega.com.
authors.forename: Jeanne M
authors.initials: JM
authors.lastname: Bourdeau
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: benjamin.krenke@promega.com.
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Sprecher
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: cindy.sprecher@promega.com.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Krenke
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: doug.storts@promega.com.
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Storts
mesh_terms: D030541:Databases, Genetic; D053803:Forensic Genetics; D006801:Humans; D018895:Microsatellite Repeats
keywords: Forensic science; PowerPlex(R); Short tandem repeat; Validation