title: A Comparative Serological Study of Toxoplasmosis in Pregnant Women by CLIA and ELISA Methods in Chalus City Iran.
abstract: BACKGROUND
  Toxoplasmosis is the most common disease in humans and animals (zoonosis) caused by the protozoan parasite Toxoplasma gondii. The disease is usually appeared as asymptomatic in immunocompromised individuals but its most common symptom is lymphadenopathy. Shortly before or during the first trimester of pregnancy, this disease can be transferred to the fetus and cause serious infection in the fetus. In late pregnancy (third trimester), the complications of this infection is very low or unsigned. Due to the absence of non-specific clinical symptoms or slight infection in pregnant women, prenatal diagnosis is often impossible.
  
  
  OBJECTIVE
  Since no research compared these two methods, we decided to compare these methods and determine which method works better for diagnosis of toxoplasmosis.
  
  
  METHODS
  In this study, 50 pregnant women who referred to the Chalus Health Center laboratory were included and the blood samples were tested for presence of IgG and IgM antibodies of Toxoplasma gondii by both ELISA and Chemiluminescence methods.
  
  
  RESULTS
  Of the 50 samples tested by the ELISA method, 26 samples (52%) were positive for IgG . No samples were positive for IgM. Of the 50 samples tested by the Chemiluminescence method, 28 samples (56%) were positive for IgG. No samples were positive for IgM.
  
  
  CONCLUSIONS
  A significant relationship between the age of the youngest child and the infection rate was seen. No significant correlation between age, number of individuals in the household, number of children, location, type of construction, consumption of greens, the way of greens and meat consumption, drug use, history of stillbirth and infection levels was seen.
authors.affiliation: Department of Microbiology, Islamic Azad University, Science and Research Ayatollah Amoli Branch, Amol, IR.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Elahian Firouz
authors.affiliation: Department of Microbiology, Islamic Azad University, Science and Research Ayatollah Amoli Branch, Amol, IR.
authors.forename: Hami
authors.initials: H
authors.lastname: Kaboosi
authors.affiliation: Department of Infectious Disease, Iran Blood Transfer Organization, Sari, IR Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Faghih Nasiri
authors.affiliation: ENT-HNS Research Center, Hazrate Rasool Hospital, Iran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Seyed Saleh
authors.initials: SS
authors.lastname: Tabatabaie
authors.affiliation: Department of Physiology, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Farideh
authors.initials: F
authors.lastname: Golhasani-Keshtan
authors.affiliation: Department of Mycology, Islamic Azad University, Science and Research Ayatollah Amoli Branch, Amol, IR Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Zaboli
keywords: Congenital Infection; Enzyme-Linked Immunosorbent Assay; Toxoplasma; Toxoplasmosis in Pregnancy