title: Cytokine-induced neutrophil chemotaxis assay.
abstract: Chemotaxis is directed migration of a cell type to a distant chemoattractant. When this chemoattractant is a cytokine, the term chemokine is often used. Chemotaxis by neutrophils, specifically polymorphonuclear leukocytes (PMNs), plays a critical role in the innate immune response. On an equimolar basis, interleukin-8 (IL-8) is one of the most potent PMN chemokines known. This chapter describes an in vitro chemotaxis technique using PMNs and IL-8 which can serve the investigator as an established model from which new studies can be developed.
authors.affiliation: Division of Neonatal-Perinatal Medicine, Stony Brook Long Island Children's Hospital, Stony Brook University, Health Science Center Building, 11th Floor, Room 060, Stony Brook, NY, 11794, USA, Dennis.Davidson@stonybrookmedicine.edu.
authors.forename: Dennis
authors.initials: D
authors.lastname: Davidson
authors.forename: Hardik
authors.initials: H
authors.lastname: Patel
mesh_terms: D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D015194:Diffusion Chambers, Culture; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D016209:Interleukin-8