title: Effects of Selol 5% supplementation on the activity or concentration of antioxidants and malondialdehyde level in the blood of healthy mice.
abstract: BACKGROUND
  Selol is a novel organoselenium Se(IV) compound. It reveals lower potential of toxicity than sodium selenite and does not exhibit mutagenic activity. Its antioxidant and anticancer properties including overcoming cancer cell resistance to standard therapy of the drug were proven. This is the first publication describing the influence of Selol 5% on the activity of blood antioxidant status in vivo.
  
  
  METHODS
  We investigated the influence of Selol 5% short-term (24h) and long-term (28 days) administration on the activity of antioxidant enzymes, including the main selenoenzymes, in healthy mice plasma and erythrocytes. Plasma oxygen radical absorbance capacity value (ORAC) and the concentration of malonyldialdehyde (MDA) in plasma as a biomarker of oxidative stress as well as the value of selenium (Se) concentration in erythrocytes were shown.
  
  
  RESULTS
  A significant increase of the selenium dependent glutathione peroxidase (Se-GSHPx) activity in plasma and erythrocytes, plasma selenoprotein P concentration, ORAC values, and Se concentration were observed during long-term supplementation as well as after Selol 5% single-dose administration, with two distinct increases of activity a few hours after the beginning of the experiment and before its end. We found a decreased thioredoxin reductase (THRR) activity and an increased MDA level during Selol 5% long-term supplementation. Glutathione S-transferase activity (GST) remained unchanged.
  
  
  CONCLUSIONS
  Selol 5% supplementation in vivo affects the selenoenzymes activities as well as the antioxidant status of plasma and erythrocytes. Selol 5% is an inhibitor of thioredoxin reductase activity, which can be important in anticancer therapy.
authors.affiliation: Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland. Electronic address: malgorzata.bogucka@wum.edu.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sochacka
authors.affiliation: Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Giebułtowicz
authors.affiliation: Department of Pharmacology, National Medicines Institute, Warsaw, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Remiszewska
authors.affiliation: Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland; Department of Pharmaceutical Chemistry, National Medicines Institute, Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Suchocki
authors.affiliation: Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Wroczyński
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D019587:Dietary Supplements; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D051379:Mice; D018384:Oxidative Stress; D018036:Selenium Compounds / Q000008:administration & dosage*; D051149:Selenoprotein P / Q000097:blood; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018036:Selenium Compounds; D051149:Selenoprotein P; C105370:selol; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013880:Thioredoxin-Disulfide Reductase; D005982:Glutathione Transferase
keywords: Glutathione S-transferase; Glutathione peroxidase; Malonyldialdehyde; Oxygen radical absorbance capacity; Selenium; Selol; Thioredoxin reductase