title: Facile LC-UV methods for simultaneous monitoring of ciprofloxacin and rosuvastatin in API, formulations and human serum.
abstract: An efficient, selective and cost-effective liquid chromatographic assay was developed and validated for the simultaneous quantification of ciprofloxacin and rosuvastatin in Active Pharmaceutical Ingredients (API), pharmaceutical formulations and in human serum. The chromatographic system consisted of mobile phase methanol-water, 90:10 v/v at pH 3.0 adjusted with o-phosphoric acid, pumped at 1.0 mL/min through a prepacked Purospher Star C18 (5 µm, 25 × 0.46 cm) column and effluent was monitored at the isosbestic point (255 nm) as well as at the λmax of individual drugs (243 and 271 nm). The method was validated over a linear concentration range of 0.25-15 µg/mL for ciprofloxacin and 0.33-20 µg/mL for rosuvastatin (r(2)  ≥ 0.999). The ranges of reliable response (limits of detection and quantitation) for ciprofloxacin were 3-15 and 9-45 ng/mL and 17-29 and 52-88 ng/mL, respectively, for rosuvastatin in all API, pharmaceutical formulations and human serum. Analytical recovery from human serum was >98% and relative standard deviation (RSD) was <2. The accuracies were 97.13-102.55 and 97.41-101.31% and precisions in RSD were 0.04-1.90 and 0.02-1.23% for ciprofloxacin and rosuvastatin, respectively. No matrix interferences, ion suppression/enhancement and carry-over were detected. The total assay run time was less than 5 min. In another study, for optimum performance the detector was programmed for multiwavelength scanning at the absorption maxima of each component. Consequently, the linearity range was improved and limit of detection and quantitation values were down to 1-4 and 4-12 ng/mL for ciprofloxacin and 3-5 and 9-15 ng/mL for rosuvastatin, respectively. The validation parameters fitted ICH guidelines through the isosbestic and individual λmax approach. The small sample volume and simplicity of preparation make this method suitable for use in human serum samples, pharmaceutical formulations, quality control, drug-drug interaction studies, clinical laboratories, drug research centers and forensic medical centers.
authors.affiliation: Department of Chemistry, University of Karachi, Karachi, 75270, Pakistan.
authors.forename: M Saeed
authors.initials: MS
authors.lastname: Arayne
authors.forename: Najma
authors.initials: N
authors.lastname: Sultana
authors.forename: Arman
authors.initials: A
authors.lastname: Tabassum
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000097:blood*; D005464:Fluorobenzenes / Q000097:blood*; D006801:Humans; D008297:Male; D011743:Pyrimidines / Q000097:blood*; D015203:Reproducibility of Results; D000068718:Rosuvastatin Calcium; D013449:Sulfonamides / Q000097:blood*; D014466:Ultraviolet Rays; D055815:Young Adult
chemical_list: D005464:Fluorobenzenes; D011743:Pyrimidines; D013449:Sulfonamides; D002939:Ciprofloxacin; D000068718:Rosuvastatin Calcium
keywords: ciprofloxacin; liquid chromatography; rosuvastatin