title: Exercise-induced expression of peroxisome proliferator-activated receptor γ coactivator-1α isoforms in skeletal muscle of endurance-trained males.
abstract: The aim of the present study was to investigate the effect of acute aerobic exercise on the expression of PGC-1α transcript variants in human skeletal muscle. Seven endurance-trained athletes performed a 90-min cycling test (62% of VO2max). At resting state, the levels of N-truncated (NT)-PGC-1α and PGC-1α exon 1a-derived transcripts were significantly higher (>20-fold; P<0.05) than those of PGC-1α exon 1b- and 1c-derived transcripts. Acute exercise did not change the PGC-1α exon 1a-derived expression level, but it did increase the expression level of NT-PGC-1α mRNAs 6-fold, and the expression levels of PGC-1α exon 1b- and 1c-derived mRNAs>200-fold (P<0.05). We conclude that NT-PGC-1α transcript expression in resting muscle and after acute moderate-intensity exercise constituted a significant share of total PGC-1α expression. The exercise led to a higher level of PGC-1α expression from alternative promoters (exon 1b- and 1c-derived mRNA) than from the canonical proximal promoter (exon 1a-derived mRNA).
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, 76A Khoroshevskoe Shosse, Moscow, 123007, Russia, danil-popov@yandex.ru.
authors.forename: Daniil V
authors.initials: DV
authors.lastname: Popov
authors.forename: Anton V
authors.initials: AV
authors.lastname: Bachinin
authors.forename: Evgeny A
authors.initials: EA
authors.lastname: Lysenko
authors.forename: Tatiana F
authors.initials: TF
authors.lastname: Miller
authors.forename: Olga L
authors.initials: OL
authors.lastname: Vinogradova
mesh_terms: D015444:Exercise / Q000502:physiology*; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010807:Physical Endurance / Q000502:physiology*; D020033:Protein Isoforms / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D020033:Protein Isoforms; D012333:RNA, Messenger; D014157:Transcription Factors