title: New Peptide-based antimicrobials for tackling drug resistance in bacteria: single-cell fluorescence imaging.
abstract: New peptide molecules with metal binding abilities proved to be active against multidrug resistant clinical isolates. One of them labeled with a dansylated lysine has been imaged inside single-multidrug resistant bacteria cells by deep ultraviolet fluorescence, showing a heterogeneous subcellular localization. The fluorescence intensity is clearly related to the accumulation of the drug inside the bacteria, being dependent both on its concentration and on the incubation time with cells.
authors.affiliation: UMR-MD1, Aix-Marseille Université IRBA, Faculte de Medecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Pagès
authors.affiliation: DISCO Beamline, Synchrotron Soleil , L'Orme des Merisiers, 91192 Gif-sur-Yvette, France.
authors.forename: Slavka
authors.initials: S
authors.lastname: Kascàkovà
authors.affiliation: UMR-MD1, Aix-Marseille Université IRBA, Faculte de Medecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Maigre
authors.affiliation: UMR 8601, LCBPT, Université Paris Descartes , PRES Paris cité, 45 rue des Sts Pères, 75270 Paris Cedex 06, France.
authors.forename: Anas
authors.initials: A
authors.lastname: Allam
authors.affiliation: UMR 8601, LCBPT, Université Paris Descartes , PRES Paris cité, 45 rue des Sts Pères, 75270 Paris Cedex 06, France.
authors.forename: Mickael
authors.initials: M
authors.lastname: Alimi
authors.affiliation: UMR-MD1, Aix-Marseille Université IRBA, Faculte de Medecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Chevalier
authors.affiliation: UMR 8601, LCBPT, Université Paris Descartes , PRES Paris cité, 45 rue des Sts Pères, 75270 Paris Cedex 06, France.
authors.forename: Erwan
authors.initials: E
authors.lastname: Galardon
authors.affiliation: DISCO Beamline, Synchrotron Soleil , L'Orme des Merisiers, 91192 Gif-sur-Yvette, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Réfrégiers
authors.affiliation: UMR 8601, LCBPT, Université Paris Descartes , PRES Paris cité, 45 rue des Sts Pères, 75270 Paris Cedex 06, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Artaud
keywords: Multidrug resistant; amphipatic peptide; fluorescence imaging; metal binding group; microspectrofluorimetry