title: Construction of an efficient Escherichia coli whole-cell biocatalyst for D-mannitol production.
abstract: Mannitol is a six carbon sugar alcohol that finds applications in the pharmaceutical and food industries. A novel Escherichia coli strain capable of converting D-glucose to D-mannitol has been constructed, wherein native mannitol-1-phosphate dehydrogenase (MtlD) and codon-optimized Eimeria tenella mannitol-1-phosphatase (M1Pase) have been overexpressed. Codon-optimized Pseudomonas stutzeri phosphite dehydrogenase (PtxD) was overexpressed for cofactor (NADH) regeneration with the concomitant oxidation of phosphite to phosphate. Whole-cell biotransformation using resting cells in a medium containing D-glucose and equimolar sodium phosphite resulted in d-mannitol yield of 87 mol%. Thus, production of an industrially relevant biochemical without using complex media components and elaborate process control mechanisms has been demonstrated.
authors.affiliation: DBT-ICT-Centre for Energy Biosciences, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (East), Mumbai 400019, Maharashtra, India. Electronic address: ss.reshamwala@ictmumbai.edu.in.
authors.forename: Shamlan M S
authors.initials: SM
authors.lastname: Reshamwala
authors.affiliation: DBT-ICT-Centre for Energy Biosciences, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (East), Mumbai 400019, Maharashtra, India.
authors.forename: Sandip K
authors.initials: SK
authors.lastname: Pagar
authors.affiliation: DBT-ICT-Centre for Energy Biosciences, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (East), Mumbai 400019, Maharashtra, India.
authors.forename: Vishal S
authors.initials: VS
authors.lastname: Velhal
authors.affiliation: DBT-ICT-Centre for Energy Biosciences, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (East), Mumbai 400019, Maharashtra, India.
authors.forename: Vijay M
authors.initials: VM
authors.lastname: Maranholakar
authors.affiliation: DBT-ICT-Centre for Energy Biosciences, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (East), Mumbai 400019, Maharashtra, India.
authors.forename: Vishal G
authors.initials: VG
authors.lastname: Talangkar
authors.affiliation: DBT-ICT-Centre for Energy Biosciences, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (East), Mumbai 400019, Maharashtra, India; Department of Chemical Engineering, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (East), Mumbai 400019, Maharashtra, India.
authors.forename: Arvind M
authors.initials: AM
authors.lastname: Lali
mesh_terms: D055162:Biocatalysis; D053898:Biosynthetic Pathways / Q000235:genetics; D001711:Biotransformation; D003062:Codon / Q000235:genetics; D016786:Eimeria tenella / Q000201:enzymology; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism; D008353:Mannitol / Q000737:chemistry / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D017905:Phosphites / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism; D044223:Pseudomonas stutzeri / Q000201:enzymology; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism
chemical_list: D003062:Codon; D005636:Fructosephosphates; D017905:Phosphites; D009243:NAD; D008353:Mannitol; C027618:fructose-6-phosphate; D013401:Sugar Alcohol Dehydrogenases; C021544:mannitol-1-phosphate dehydrogenase; D009247:NADH, NADPH Oxidoreductases; C429539:NAD phosphite oxidoreductase; D010744:Phosphoric Monoester Hydrolases; C021543:mannitol-1-phosphatase; D005947:Glucose
keywords: Biotransformation; Cofactor regeneration; Resting cells; Whole-cell biocatalyst; d-Mannitol production