title: The histone variant composition of centromeres is controlled by the pericentric heterochromatin state during the cell cycle.
abstract: Correct chromosome segregation requires a unique chromatin environment at centromeres and in their vicinity. Here, we address how the deposition of canonical H2A and H2A.Z histone variants is controlled at pericentric heterochromatin (PHC). Whereas in euchromatin newly synthesized H2A and H2A.Z are deposited throughout the cell cycle, we reveal two discrete waves of deposition at PHC - during mid to late S phase in a replication-dependent manner for H2A and during G1 phase for H2A.Z. This G1 cell cycle restriction is lost when heterochromatin features are altered, leading to the accumulation of H2A.Z at the domain. Interestingly, compromising PHC integrity also impacts upon neighboring centric chromatin, increasing the amount of centromeric CENP-A without changing the timing of its deposition. We conclude that the higher-order chromatin structure at the pericentric domain influences dynamics at the nucleosomal level within centromeric chromatin. The two different modes of rearrangement of the PHC during the cell cycle provide distinct opportunities to replenish one or the other H2A variant, highlighting PHC integrity as a potential signal to regulate the deposition timing and stoichiometry of histone variants at the centromere.
authors.affiliation: Institut Curie, Centre de Recherche, Paris, 75248 France CNRS, UMR3664, Paris, 75248 France Equipe Labellisée Ligue contre le Cancer, UMR3664, Paris, 75248 France UPMC, UMR3664, Paris, 75248 France Sorbonne University, PSL, 75006 Paris, France.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Boyarchuk
authors.affiliation: Institut Curie, Centre de Recherche, Paris, 75248 France CNRS, UMR3664, Paris, 75248 France Equipe Labellisée Ligue contre le Cancer, UMR3664, Paris, 75248 France UPMC, UMR3664, Paris, 75248 France Sorbonne University, PSL, 75006 Paris, France.
authors.forename: Dan
authors.initials: D
authors.lastname: Filipescu
authors.affiliation: Institut Curie, Centre de Recherche, Paris, 75248 France CNRS, UMR3664, Paris, 75248 France Equipe Labellisée Ligue contre le Cancer, UMR3664, Paris, 75248 France UPMC, UMR3664, Paris, 75248 France Sorbonne University, PSL, 75006 Paris, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vassias
authors.affiliation: Institut Curie, Centre de Recherche, Paris, 75248 France CNRS, UMR3664, Paris, 75248 France Equipe Labellisée Ligue contre le Cancer, UMR3664, Paris, 75248 France UPMC, UMR3664, Paris, 75248 France Sorbonne University, PSL, 75006 Paris, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Cantaloube
authors.affiliation: Institut Curie, Centre de Recherche, Paris, 75248 France CNRS, UMR3664, Paris, 75248 France Equipe Labellisée Ligue contre le Cancer, UMR3664, Paris, 75248 France UPMC, UMR3664, Paris, 75248 France Sorbonne University, PSL, 75006 Paris, France genevieve.almouzni@curie.fr.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Almouzni
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle; D002503:Centromere / Q000235:genetics; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D006570:Heterochromatin; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D046912:Multiprotein Complexes / Q000378:metabolism
chemical_list: D001324:Autoantigens; C000619701:Cenpa protein, mouse; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D006570:Heterochromatin; D006657:Histones; D046912:Multiprotein Complexes; C077020:histone H2A.F-Z
keywords: CENP-A; Cell cycle; Centromere; H2A.Z; Heterochromatin