title: Unidirectional P-body transport during the yeast cell cycle.
abstract: P-bodies belong to a large family of RNA granules that are associated with post-transcriptional gene regulation, conserved from yeast to mammals, and influence biological processes ranging from germ cell development to neuronal plasticity. RNA granules can also transport RNAs to specific locations. Germ granules transport maternal RNAs to the embryo, and neuronal granules transport RNAs long distances to the synaptic dendrites. Here we combine microfluidic-based fluorescent microscopy of single cells and automated image analysis to follow p-body dynamics during cell division in yeast. Our results demonstrate that these highly dynamic granules undergo a unidirectional transport from the mother to the daughter cell during mitosis as well as a constrained "hovering" near the bud site half an hour before the bud is observable. Both behaviors are dependent on the Myo4p/She2p RNA transport machinery. Furthermore, single cell analysis of cell size suggests that PBs play an important role in daughter cell growth under nutrient limiting conditions.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Garmendia-Torres
authors.affiliation: Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg; National Center for Microscopy and Imaging Research, University of California San Diego, La Jolla, California, United States of America.
authors.forename: Alexander
authors.initials: A
authors.lastname: Skupin
authors.affiliation: Institute for Systems Biology, Seattle, Washington, United States of America.
authors.forename: Sean A
authors.initials: SA
authors.lastname: Michael
authors.affiliation: Tampere University of Technology, Pori, Finland; BioMediTech, University of Tampere, Tampere, Finland.
authors.forename: Pekka
authors.initials: P
authors.lastname: Ruusuvuori
authors.affiliation: Physics and Bioengineering, University of Washington, Seattle, Washington, United States of America.
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Kuwada
authors.affiliation: Centre for High-Throughput Biology, Department of Physics and Astronomy, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Didier
authors.initials: D
authors.lastname: Falconnet
authors.affiliation: Institute for Systems Biology, Seattle, Washington, United States of America; Molecular and Cellular Biology Program, University of Washington, Seattle, Washington, United States of America.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Cary
authors.affiliation: Centre for High-Throughput Biology, Department of Physics and Astronomy, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Carl
authors.initials: C
authors.lastname: Hansen
authors.affiliation: Physics and Bioengineering, University of Washington, Seattle, Washington, United States of America.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Wiggins
authors.affiliation: Molecular and Cellular Biology Program, University of Washington, Seattle, Washington, United States of America; Pacific Northwest Diabetes Research Institute, Seattle, Washington, United States of America.
authors.forename: Aimée M
authors.initials: AM
authors.lastname: Dudley
mesh_terms: D001692:Biological Transport; D002453:Cell Cycle; D003594:Cytoplasmic Granules / Q000378:metabolism*; D044085:Microfluidics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000254:growth & development