title: Fermentative production of nervonic acid by Mortierella capitata RD000969.
abstract: A high-nervonic acid (cis-15-tetracosenoic acid, C24:1, n-9)-producing filamentous fungus of the Mortierella species was discovered among soil filamentous fungi. The filamentous fungal strain -RD000969- was isolated from soil collected in Kanagawa Prefecture (Japan) and was found to accumulate nervonic acid at a rate of 6.94% of the total cellular fatty acids. The base sequences of 28S rDNA D1/D2 and ITS 5.8S rDNA showed 100% homology with Mortierella capitata CBS 293.96. In addition to nervonic acid, strain RD000969 produced a large amount of long-chain monounsaturated fatty acids (C20:1, 12.22%; C22:1, 4.07%; C26:1, 5.91%) and a small amount of ultra-long-chain fatty acids (C28:1, 0.44%; C30:1, 0.06%; C32:1, trace). In the fungal cells, 98.87% of nervonic acid was localized at the sn-1,3 position of triacylglycerol. Nervonic acid production was maximum (186.3 mg·L(-1)) when the fungus was cultured in potato dextrose (PD) medium containing yeast extract, CaCl2, and MgSO4·7H2O.
authors.affiliation: Department of Applied Biological Chemistry, Kinki University.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Umemoto
authors.forename: Keigo
authors.initials: K
authors.lastname: Sawada
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kurata
authors.forename: Shohei
authors.initials: S
authors.lastname: Hamaguchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsukahara
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishiguro
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kishimoto
mesh_terms: D001483:Base Sequence; D003470:Culture Media / Q000737:chemistry; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology*; D005947:Glucose; D007564:Japan; D020094:Mortierella / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D014280:Triglycerides / Q000737:chemistry
chemical_list: D003470:Culture Media; D005229:Fatty Acids, Monounsaturated; D014280:Triglycerides; C013147:nervonic acid; D005947:Glucose