title: Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA demethylation.
abstract: DNA methylation is a reversible epigenetic mark regulating genome stability and function in many eukaryotes. In Arabidopsis, active DNA demethylation depends on the function of the ROS1 subfamily of genes that encode 5-methylcytosine DNA glycosylases/lyases. ROS1-mediated DNA demethylation plays a critical role in the regulation of transgenes, transposable elements and some endogenous genes; however, there have been no reports of clear developmental phenotypes in ros1 mutant plants. Here we report that, in the ros1 mutant, the promoter region of the peptide ligand gene EPF2 is hypermethylated, which greatly reduces EPF2 expression and thereby leads to a phenotype of overproduction of stomatal lineage cells. EPF2 gene expression in ros1 is restored and the defective epidermal cell patterning is suppressed by mutations in genes in the RNA-directed DNA methylation pathway. Our results show that active DNA demethylation combats the activity of RNA-directed DNA methylation to influence the initiation of stomatal lineage cells.
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Chizuko
authors.initials: C
authors.lastname: Yamamuro
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Miki
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Botany and Plant Science, Center for Plant Cell Biology, University of California, Riverside, California 92521, USA.
authors.forename: Zhenbiao
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Waksman Institute of Microbiology, Rutgers University, Piscataway, New Jersey 08854, USA.
authors.forename: Juan
authors.initials: J
authors.lastname: Dong
authors.affiliation: 1] Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China [2] Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
mesh_terms: D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D044127:Epigenesis, Genetic; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D019441:Plant Epidermis; D054046:Plant Stomata / Q000166:cytology*; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C542689:EPF2 protein, Arabidopsis; D009687:Nuclear Proteins; C471271:ROS1 protein, Arabidopsis; D014157:Transcription Factors