title: TtcA a new tRNA-thioltransferase with an Fe-S cluster.
abstract: TtcA catalyzes the post-transcriptional thiolation of cytosine 32 in some tRNAs. The enzyme from Escherichia coli was homologously overexpressed in E. coli. The purified enzyme is a dimer containing an iron-sulfur cluster and displays activity in in vitro assays. The type and properties of the cluster were investigated using a combination of UV-visible absorption, EPR and Mössbauer spectroscopy, as well as by site-directed mutagenesis. These studies demonstrated that the TtcA enzyme contains a redox-active and oxygen-sensitive [4Fe-4S] cluster, chelated by only three cysteine residues and absolutely essential for activity. TtcA is unique tRNA-thiolating enzyme using an iron-sulfur cluster for catalyzing a non-redox reaction.
authors.affiliation: University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Bouvier
authors.affiliation: University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.
authors.forename: Natty
authors.initials: N
authors.lastname: Labessan
authors.affiliation: University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Clémancey
authors.affiliation: University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Latour
authors.affiliation: University of Grenoble Alpes, INAC, SCIB, F-38000 Grenoble, France CEA, iNAC, SCIB, F-38054 Grenoble, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Ravanat
authors.affiliation: University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France Laboratoire de Chimie des Processus Biologiques, UMR 8229 Collège de France/CNRS/UPMC, 11 place Marcellin-Berthelot, Paris, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Fontecave
authors.affiliation: University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France mohamed.atta@cea.fr.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Atta
mesh_terms: D003001:Cloning, Molecular; D003545:Cysteine / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015870:Gene Expression; D054477:Glutaredoxins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013466:Sulfurtransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D054477:Glutaredoxins; D007506:Iron-Sulfur Proteins; D013466:Sulfurtransferases; C000591238:TtcA protein, E coli; D003545:Cysteine