title: In situ tracking the intracellular delivery of antisense oligonucleotides by fluorescein doped silica nanoparticles.
abstract: Antisense oligonucleotides (ASOs) are often utilized to interfere with gene expression at mRNA level for cancer treatment. Here, we synthesized fluorescein doped silica nanoparticles (FSNPs) and coated them by polyethyleneimine (PEI) for carrying ASOs. Agarose gel electrophoresis proved that PEI/FSNPs could load ASOs by a weight ratio as high as 30:1. We tracked the delivery process of ASOs from the ASOs/PEI/FSNPs composites to HeLa cells in situ by the confocal laser scanning microscopy (CLSM) techniques, including nuclear staining and Z-axis scanning. We found the ASOs/PEI/FSNPs composites exhibited their biological effects at specific intracellular localization, and the fluorescence of the FSNPs showed the dynamic delivery process in the cells.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, China.
authors.forename: Tian-Yi
authors.initials: TY
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, China. Electronic address: hmxiong@fudan.edu.cn.
authors.forename: Huan-Ming
authors.initials: HM
authors.lastname: Xiong
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China. Electronic address: jlkong@fudan.edu.cn.
authors.forename: Ji-Lie
authors.initials: JL
authors.lastname: Kong
mesh_terms: D000327:Adsorption; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation; D019793:Fluorescein / Q000008:administration & dosage / Q000737:chemistry; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage* / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D012822:Silicon Dioxide / Q000008:administration & dosage* / Q000737:chemistry; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D005456:Fluorescent Dyes; D051034:Inhibitor of Apoptosis Proteins; D016376:Oligonucleotides, Antisense; D000077022:Survivin; D012822:Silicon Dioxide; D011094:Polyethyleneimine; D019793:Fluorescein
keywords: Antisense oligonucleotides; Confocal laser scanning microscopy; Fluorescein doped silica nanoparticles; In situ tracking