title: A glass microchip device for conducting serological survey of West Nile viral antibodies.
abstract: Serological surveys are vital to determining the prevalence of a disease and/or the immunity status against it in any population. However, the relatively large sample volume requirement (1-10 mL) in traditional serum-based assays demands that blood draw camps be set up by medical professionals to obtain samples for these studies which significantly increases the time and cost associated with them. Here we address these drawbacks of a serosurvey by reducing the whole blood requirement in its diagnostic procedures down to 10 μL using the microfluidic platform. Such a miniaturization approach was demonstrated in our current work by developing a microchip based serological device for determining the serum levels of West Nile (WN) viral antibodies (IgG and IgM) to assess the immunity status against WN virus in Fremont County, Wyoming. Enzyme-linked immunosorbent assays (ELISA) were developed for these target analytes in glass microchannels to accomplish this task using antibodies/assay reagents purchased from commercial sources. The reported assays were directly quantitated using a fluorescence microplate reader which to our knowledge is the first account of signal measurement in a microchip based ELISA procedure using this standard instrument. To enable this quantitation method, the assay channels on our device were spaced identically as the wells on a commercial microplate, and a holder having the dimensions of this plate was used to accommodate the microchips. Our microfluidic assays showed an excellent correlation with the results from the microwell plate based experiments for significantly lower incubation periods and using only 3 μL of the ELISA reagents.
authors.affiliation: Department of Chemistry (Dept. # 3838), University of Wyoming, 1000 East University Avenue, Laramie, WY, 82071, USA.
authors.forename: Javier
authors.initials: J
authors.lastname: Pena
authors.forename: Steven J
authors.initials: SJ
authors.lastname: McAllister
authors.forename: Debashis
authors.initials: D
authors.lastname: Dutta
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D005898:Glass; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D056656:Lab-On-A-Chip Devices; D014902:West Nile virus
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M