title: Exercise-induced regulation of matrix metalloproteinases in the skeletal muscle of subjects with type 2 diabetes.
abstract: Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMP) play a critical role during vascular remodelling, in both health and disease. Impaired MMP regulation is associated with many diabetes-related complications. This study examined whether exercise-induced regulation of MMPs is maintained in the skeletal muscle of patients with uncomplicated type 2 diabetes (T2DM). Subjects [12 T2DM, 9 healthy control subjects (CON)] underwent 8 weeks of physical training. Messenger RNA (mRNA) was measured at baseline, during and after 8 weeks of training. Protein was measured pre- and post-training. At baseline, there were no effects of diabetes on MMP or TIMP mRNA or protein. mRNA and protein response to training was similar in both groups, except active MMP-2 protein was elevated post training in T2DM only. Our results indicate that exercise-induced stimulation of MMPs is preserved in skeletal muscle of patients with T2DM. This early stage of diabetes may provide an opportunity for intervention and prevention of complications.
authors.affiliation: Xlab, Center for Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark Department of Kinesiology and Physical Education, McGill University, Montreal, QC, Canada celena.scheede@mcgill.ca.
authors.forename: Celena
authors.initials: C
authors.lastname: Scheede-Bergdahl
authors.affiliation: Department of Exercise Science, Concordia University, Montreal, QC, Canada.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bergdahl
authors.affiliation: Institute of Sports Medicine, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Peter
authors.initials: P
authors.lastname: Schjerling
authors.affiliation: Department of Biomedical Sciences/CFIM, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Klaus
authors.initials: K
authors.lastname: Qvortrup
authors.affiliation: Institute of Sports Medicine, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Satu O
authors.initials: SO
authors.lastname: Koskinen
authors.affiliation: Xlab, Center for Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Flemming
authors.initials: F
authors.lastname: Dela
mesh_terms: D003718:Denmark; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology / Q000628:therapy*; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism; D005081:Exercise Therapy; D018093:Gelatinases / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000503:physiopathology / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: D004792:Enzyme Precursors; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D018093:Gelatinases; D020782:Matrix Metalloproteinases; C069860:progelatinase; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14
keywords: Matrix metalloproteinase; capillary basement membrane; exercise; extracellular matrix; type 2 diabetes