title: Analyzing Cdc2/Cdk1 activation during stress response in Schizosaccharomyces pombe.
abstract: Stress leads to multiple changes in the physiology of the cell. One of the most important is the adaptation of the cell cycle to the changing conditions of the environment. Cellular responses after stress can be followed by cellular synchronization previous to the insult. In this chapter, we use centrifugal elutriation to synchronize Schizosaccharomyces pombe cells and outline methods to investigate the hallmarks of cell cycle progression upon stress. These include analyses of cyclin-dependent kinase phosphorylation and cell size change.
authors.affiliation: Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas (UAM-CSIC), Universidad Autónoma de Madrid, Cantoblanco, Madrid, 28049, Spain, marodriguez@cbm.uam.es.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Rodríguez-Gabriel
mesh_terms: D016203:CDC2 Protein Kinase / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000295:instrumentation; D002453:Cell Cycle; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods; D004867:Equipment Design; D012568:Schizosaccharomyces / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D029702:Schizosaccharomyces pombe Proteins; D016203:CDC2 Protein Kinase; C469447:cdc2 protein, S pombe