title: The presence of soluble carbonic anhydrase in the thylakoid lumen of chloroplasts from Arabidopsis leaves.
abstract: Supernatant obtained after high-speed centrifugation of disrupted thylakoids that had been washed free from extrathylakoid carbonic anhydrases demonstrated carbonic anhydrase activity that was inhibited by the specific inhibitors acetazolamide and ethoxyzolamide. A distinctive feature of the effect of Triton X-100 on this activity also suggested that the source of the activity is a soluble protein. Native electrophoresis of a preparation obtained using chromatography with agarose/mafenide as an affinity sorbent revealed one protein band with carbonic anhydrase activity. The same protein was revealed in a mutant deficient in soluble stromal carbonic anhydrase β-CA1, and this indicated that the newly revealed carbonic anhydrase is not a product of the At3g01500 gene. These data imply the presence of soluble carbonic anhydrase in the thylakoid lumen of higher plants.
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino 142290, Russia.
authors.forename: Tat'yana
authors.initials: T
authors.lastname: Fedorchuk
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino 142290, Russia. Electronic address: rudenko_n@rambler.ru.
authors.forename: Natalia
authors.initials: N
authors.lastname: Rudenko
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino 142290, Russia.
authors.forename: Lyudmila
authors.initials: L
authors.lastname: Ignatova
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino 142290, Russia.
authors.forename: Boris
authors.initials: B
authors.lastname: Ivanov
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology*; D018515:Plant Leaves / Q000201:enzymology*; D020524:Thylakoids / Q000201:enzymology*
chemical_list: D002256:Carbonic Anhydrases
keywords: Arabidopsis thaliana; Carbonic anhydrase; Thylakoid lumen; Thylakoids