title: Quantitative temporal viromics: an approach to investigate host-pathogen interaction.
abstract: A systematic quantitative analysis of temporal changes in host and viral proteins throughout the course of a productive infection could provide dynamic insights into virus-host interaction. We developed a proteomic technique called "quantitative temporal viromics" (QTV), which employs multiplexed tandem-mass-tag-based mass spectrometry. Human cytomegalovirus (HCMV) is not only an important pathogen but a paradigm of viral immune evasion. QTV detailed how HCMV orchestrates the expression of >8,000 cellular proteins, including 1,200 cell-surface proteins to manipulate signaling pathways and counterintrinsic, innate, and adaptive immune defenses. QTV predicted natural killer and T cell ligands, as well as 29 viral proteins present at the cell surface, potential therapeutic targets. Temporal profiles of >80% of HCMV canonical genes and 14 noncanonical HCMV open reading frames were defined. QTV is a powerful method that can yield important insights into viral infection and is applicable to any virus with a robust in vitro model.
authors.affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK. Electronic address: mpw1001@cam.ac.uk.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Weekes
authors.affiliation: School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Tomasec
authors.affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Edward L
authors.initials: EL
authors.lastname: Huttlin
authors.affiliation: School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
authors.forename: Ceri A
authors.initials: CA
authors.lastname: Fielding
authors.affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: David
authors.initials: D
authors.lastname: Nusinow
authors.affiliation: School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Stanton
authors.affiliation: School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
authors.forename: Eddie C Y
authors.initials: ECY
authors.lastname: Wang
authors.affiliation: School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Aicheler
authors.affiliation: School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
authors.forename: Isa
authors.initials: I
authors.lastname: Murrell
authors.affiliation: School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
authors.forename: Gavin W G
authors.initials: GWG
authors.lastname: Wilkinson
authors.affiliation: Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Lehner
authors.affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA. Electronic address: steven_gygi@hms.harvard.edu.
authors.forename: Steven P
authors.initials: SP
authors.lastname: Gygi
mesh_terms: D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000821:virology*; D054884:Host-Pathogen Interactions; D006801:Humans; D057131:Immune Evasion; D007694:Killer Cells, Natural / Q000276:immunology; D040901:Proteomics; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis; D014773:Virology / Q000379:methods*
chemical_list: D014764:Viral Proteins