title: Genomovar assignment of Pseudomonas stutzeri populations inhabiting produced oil reservoirs.
abstract: Oil reservoirs are specific habitats for the survival and growth of microorganisms in general. Pseudomonas stutzeri which is believed to be an exogenous organism inoculated into oil reservoirs during the process of oil production was detected frequently in samples from oil reservoirs. Very little is known, however, about the distribution and genetic structure of P. stutzeri in the special environment of oil reservoirs. In this study, we collected 59 P. stutzeri 16S rRNA gene sequences that were identified in 42 samples from 25 different oil reservoirs and we isolated 11 cultured strains from two representative oil reservoirs aiming to analyze the diversity and genomovar assignment of the species in oil reservoirs. High diversity of P. stutzeri was observed, which was exemplified in the detection of sequences assigned to four known genomovars 1, 2, 3, 20 and eight unknown genomic groups of P. stutzeri. The frequent detection and predominance of strains belonging to genomovar 1 in most of the oil reservoirs under study indicated an association of genomovars of P. stutzeri with the oil field environments.
authors.affiliation: The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, Ministry of Education, China; School of Energy Resources, China University of Geosciences (Beijing), Beijing, 100083, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: She
authors.forename: Ibrahim M
authors.initials: IM
authors.lastname: Banat
authors.forename: Lu-Jun
authors.initials: LJ
authors.lastname: Chai
authors.forename: Liu-Qin
authors.initials: LQ
authors.lastname: Huang
authors.forename: Shao-Jin
authors.initials: SJ
authors.lastname: Yi
authors.forename: Zheng-Liang
authors.initials: ZL
authors.lastname: Wang
authors.forename: Hai-Liang
authors.initials: HL
authors.lastname: Dong
authors.forename: Du-Jie
authors.initials: DJ
authors.lastname: Hou
mesh_terms: D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D061329:Oil and Gas Fields / Q000382:microbiology*; D010802:Phylogeny; D044223:Pseudomonas stutzeri / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; Genomovar; Pseudomonas stutzeri; oil reservoirs