title: Changes in iron transporter divalent metal transporter 1 in proximal jejunum after gastric bypass.
abstract: OBJECTIVE
  To describe the variation that divalent metal transporter 1 (DMT1) shows in patients after Roux-en-Y gastric bypass (RYGB) surgery.
  
  
  METHODS
  Prospective and analytical study of DMT1 level at the brush border of proximal jejunum in patients having undergone RYGB surgery. The mucosa of proximal jejunum forming the gastrojejunal anastomosis was biopsied during surgery and after 6 mo later with an endoscopic biopsy. All the patients received precise instructions regarding feeding and nutritional supplementation. Both samples were processed at the same time by immunohistochemistry and western blot. Samples were analysed by a pathologist. For statistical analysis, the χ(2) and Wilcoxon tests were used.
  
  
  RESULTS
  Sixteen patients were recruited, 13 of whom completed the study. Twelve were women. Average age and body mass index (BMI) were 44.1 and 40.4, respectively. Both body weight and BMI decreased significantly during the study period, with an average percent excess weight loss (%EWL) of 60% ± 13.3% and an average percent excess BMI loss (%EBMIL) of 79.6% ± 21.6%. Only two patients presented with mild anaemia 6 mo after surgery, but their ferritin levels stayed within normal ranges. Staining for DMT1 showed a significant increase in the cytoplasm of enterocytes located at the tips of the villi (χ(2) = 6.03; P = 0.049). Nevertheless, the total quantity of DMT1 decreased significantly (Z = 2.04; P = 0.04). Associated with these results, we observed a significant increase in goblet cells in the villi 6 mo postoperatively (Z = -2.47; P = 0.013).
  
  
  CONCLUSIONS
  Six months after RYGB surgery, patients exhibit an increase in DMT1 expression in the enterocytes of the tips of the villi at the proximal jejunum.
authors.affiliation: Andrés Marambio, Guillermo Watkins, Andrea Riffo, Jaime Jans, Department of Surgery, University of Chile Clinical Hospital, Santiago 8380456, Chile.
authors.forename: Andrés
authors.initials: A
authors.lastname: Marambio
authors.affiliation: Andrés Marambio, Guillermo Watkins, Andrea Riffo, Jaime Jans, Department of Surgery, University of Chile Clinical Hospital, Santiago 8380456, Chile.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Watkins
authors.affiliation: Andrés Marambio, Guillermo Watkins, Andrea Riffo, Jaime Jans, Department of Surgery, University of Chile Clinical Hospital, Santiago 8380456, Chile.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Castro
authors.affiliation: Andrés Marambio, Guillermo Watkins, Andrea Riffo, Jaime Jans, Department of Surgery, University of Chile Clinical Hospital, Santiago 8380456, Chile.
authors.forename: Andrea
authors.initials: A
authors.lastname: Riffo
authors.affiliation: Andrés Marambio, Guillermo Watkins, Andrea Riffo, Jaime Jans, Department of Surgery, University of Chile Clinical Hospital, Santiago 8380456, Chile.
authors.forename: Roberta
authors.initials: R
authors.lastname: Zúñiga
authors.affiliation: Andrés Marambio, Guillermo Watkins, Andrea Riffo, Jaime Jans, Department of Surgery, University of Chile Clinical Hospital, Santiago 8380456, Chile.
authors.forename: Jaime
authors.initials: J
authors.lastname: Jans
authors.affiliation: Andrés Marambio, Guillermo Watkins, Andrea Riffo, Jaime Jans, Department of Surgery, University of Chile Clinical Hospital, Santiago 8380456, Chile.
authors.forename: María E
authors.initials: ME
authors.lastname: Villanueva
authors.affiliation: Andrés Marambio, Guillermo Watkins, Andrea Riffo, Jaime Jans, Department of Surgery, University of Chile Clinical Hospital, Santiago 8380456, Chile.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Díaz
mesh_terms: D000328:Adult; D000368:Aged; D000714:Anastomosis, Surgical; D001706:Biopsy; D015153:Blotting, Western; D015992:Body Mass Index; D003593:Cytoplasm / Q000378:metabolism; D019587:Dietary Supplements; D004724:Endoscopy; D020895:Enterocytes / Q000378:metabolism; D005260:Female; D015390:Gastric Bypass; D006801:Humans; D007150:Immunohistochemistry; D007583:Jejunum / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D014157:Transcription Factors / Q000378:metabolism*; D055815:Young Adult
chemical_list: C110547:DMRT1 protein; D014157:Transcription Factors
keywords: Anaemia; Bariatric surgery; Divalent metal transporter 1; Iron; Roux-en-Y gastric bypass