title: Semi-automated imaging of tissue-specific fluorescence in zebrafish embryos.
abstract: Zebrafish embryos are a powerful tool for large-scale screening of small molecules. Transgenic zebrafish that express fluorescent reporter proteins are frequently used to identify chemicals that modulate gene expression. Chemical screens that assay fluorescence in live zebrafish often rely on expensive, specialized equipment for high content screening. We describe a procedure using a standard epifluorescence microscope with a motorized stage to automatically image zebrafish embryos and detect tissue-specific fluorescence. Using transgenic zebrafish that report estrogen receptor activity via expression of GFP, we developed a semi-automated procedure to screen for estrogen receptor ligands that activate the reporter in a tissue-specific manner. In this video we describe procedures for arraying zebrafish embryos at 24-48 hours post fertilization (hpf) in a 96-well plate and adding small molecules that bind estrogen receptors. At 72-96 hpf, images of each well from the entire plate are automatically collected and manually inspected for tissue-specific fluorescence. This protocol demonstrates the ability to detect estrogens that activate receptors in heart valves but not in liver.
authors.affiliation: Department of Pharmacology and Toxicology, University of Alabama at Birmingham.
authors.forename: Shannon N
authors.initials: SN
authors.lastname: Romano
authors.affiliation: Department of Pharmacology and Toxicology, University of Alabama at Birmingham; danielg@uab.edu.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Gorelick
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001331:Automation; D004625:Embryo, Nonmammalian; D005260:Female; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008024:Ligands; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods*; D011960:Receptors, Estrogen / Q000737:chemistry / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology*
chemical_list: D008024:Ligands; D011960:Receptors, Estrogen; D049452:Green Fluorescent Proteins