title: Association of polymorphisms at LDLR locus with coronary artery disease independently from lipid profile.
abstract: Coronary artery disease (CAD) is the leading cause of mortality in many parts of the world. Genome-wide association studies (GWAS) have identified several genetic variants associated with CAD in Low-density lipoprotein receptor (LDLR) locus. This study was evaluated the possible association of genetic markers at LDLR locus with CAD irrespective to lipid profile and as well as the association of these SNPs with severity of CAD in Iranian population. Sequencing of 2 exons in LDLR gene (Exon 2, 12) and part of intron 30 of SMARCA4 gene include rs1122608, was performed in 170 Iranian patients angiographically confirmed CAD and 104 healthy controls by direct sequencing. Sullivan's scoring system was used for determining the severity of CAD in cases. Our results showed that homozygote genotypes of rs1122608 (P<0.0001), rs4300767 (P<0.005) and rs10417578 (p<0.007) SNPs have strong protective effects on the CAD. In addition, we found that rs1122608 (GT or TT) was at higher risk of three vessel involvement compared to single vessels affecting (P=0.01).
authors.affiliation: Department of Genetics, Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran hjam1358@yahoo.co.uk.
authors.forename: Seyed Hamid
authors.initials: SH
authors.lastname: Jamaldini
authors.affiliation: Department of Genetics, Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran hjam1358@yahoo.co.uk.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Babanejad
authors.affiliation: Department of Genetics, Cardiogenetic Research Center, Tehran, Iran-Department of Genetics, Shahid Rajaie Cardiovascular Medical & Research Center, Tehran, Iran hjam1358@yahoo.co.uk.
authors.forename: Reza
authors.initials: R
authors.lastname: Mozaffari
authors.affiliation: Department of Genetics, Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran hjam1358@yahoo.co.uk.
authors.forename: Nooshin
authors.initials: N
authors.lastname: Nikzat
authors.affiliation: Department of Genetics, Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran hjam1358@yahoo.co.uk.
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Jalalvand
authors.affiliation: Department of Genetics, Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran hnajm12@yahoo.com.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Badiei
authors.affiliation: Department of Genetics, Cardiogenetic Research Center, Tehran, Iran-Department of Genetics, Shahid Rajaie Cardiovascular Medical & Research Center, Tehran, Iran hnajm12@yahoo.com.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Sanati
authors.affiliation: Department of Genetics, Cardiogenetic Research Center, Tehran, Iran-Department of Genetics, Shahid Rajaie Cardiovascular Medical & Research Center, Tehran, Iran hnajm12@yahoo.com.
authors.forename: Farshad
authors.initials: F
authors.lastname: Shakerian
authors.affiliation: Department of Health, Faculty of Health, Zabol University of Medical Science, Zabol, Iran hnajm12@yahoo.com.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Afshari
authors.affiliation: Department of Genetics, Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran hnajm12@yahoo.com.
authors.forename: Kimia
authors.initials: K
authors.lastname: Kahrizi
authors.affiliation: Department of Genetics, Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran hnajm12@yahoo.com.
authors.forename: Hossein
authors.initials: H
authors.lastname: Najmabadi
mesh_terms: D000368:Aged; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011973:Receptors, LDL / Q000097:blood / Q000235:genetics*
chemical_list: C573412:LDLR protein, human; D008055:Lipids; D011973:Receptors, LDL; D004247:DNA