title: Production and characterization of monoclonal antibodies against horse immunoglobulins useful for the diagnosis of equine diseases.
abstract: Monoclonal antibodies (MAbs) against horse IgG were produced by immunizing Balb/c mice with purified horse IgG and were characterized in indirect ELISA versus purified immunoglobulins from donkey, cow, buffalo, sheep, pig, and chicken. Three MAbs (1B10B6C9, 1B10B6C10, 1B10B6E9) reacted only with horse and donkey IgG and IgM and, in western blotting, were specific for the Fc fragment of equine IgG. MAb 1B10B6E9 was used in chemiluminescent immunoblotting assay for the diagnosis of dourine and in indirect immunofluorescence assay (IFA) for the diagnosis of African horse sickness and dourine.
authors.affiliation: a Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" , via Campo Boario, Teramo , Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Di Febo
authors.forename: Mirella
authors.initials: M
authors.lastname: Luciani
authors.forename: Antonella
authors.initials: A
authors.lastname: Ciarelli
authors.forename: Grazia
authors.initials: G
authors.lastname: Bortone
authors.forename: Chiara
authors.initials: C
authors.lastname: Di Pancrazio
authors.forename: Diamante
authors.initials: D
authors.lastname: Rodomonti
authors.forename: Liana
authors.initials: L
authors.lastname: Teodori
authors.forename: Manuela
authors.initials: M
authors.lastname: Tittarelli
mesh_terms: D000355:African Horse Sickness / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry* / Q000276:immunology; D004313:Dourine / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006736:Horses; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D051379:Mice
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D007074:Immunoglobulin G
keywords: equine diseases; horse immunoglobulins; immunoblotting assay; immunofluorescence assay; monoclonal antibodies