title: Mutational analysis of the Ve1 immune receptor that mediates Verticillium resistance in tomato.
abstract: Pathogenic Verticillium species are economically important plant pathogens that cause vascular wilt diseases in hundreds of plant species. The Ve1 gene of tomato confers resistance against race 1 strains of Verticillium dahliae and V. albo-atrum. Ve1 encodes an extracellular leucine-rich repeat (eLRR) receptor-like protein (RLP) that serves as a cell surface receptor for recognition of the recently identified secreted Verticillium effector Ave1. To investigate recognition of Ave1 by Ve1, alanine scanning was performed on the solvent exposed β-strand/β-turn residues across the eLRR domain of Ve1. In addition, alanine scanning was also employed to functionally characterize motifs that putatively mediate protein-protein interactions and endocytosis in the transmembrane domain and the cytoplasmic tail of the Ve1 protein. Functionality of the mutant proteins was assessed by screening for the occurrence of a hypersensitive response upon co-expression with Ave1 upon Agrobacterium tumefaciens-mediated transient expression (agroinfiltration). In order to confirm the agroinfiltration results, constructs encoding Ve1 mutants were transformed into Arabidopsis and the transgenes were challenged with race 1 Verticillium. Our analyses identified several regions of the Ve1 protein that are required for functionality.
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands; Key Laboratory of Plant Molecular Physiology, the Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Yin
authors.initials: Y
authors.lastname: Song
authors.affiliation: Key Laboratory of Plant Molecular Physiology, the Chinese Academy of Sciences, Beijing, China.
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Liu
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Bart P H J
authors.initials: BP
authors.lastname: Thomma
mesh_terms: D000409:Alanine; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D060467:Disease Resistance / Q000235:genetics*; D054884:Host-Pathogen Interactions / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics* / Q000382:microbiology*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D054730:Protein Interaction Domains and Motifs; D011971:Receptors, Immunologic / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D020172:Verticillium / Q000502:physiology*
chemical_list: D011971:Receptors, Immunologic; D000409:Alanine