title: Release and antibacterial activity of allyl isothiocyanate/β-cyclodextrin complex encapsulated in electrospun nanofibers.
abstract: Allyl isothiocyanate (AITC) is known as an efficient antibacterial agent but it has a very high volatility. Herein, AITC and AITC/β-cyclodextrin (CD)-inclusion complex (IC) incorporated in polyvinyl alcohol (PVA) nanofibers were produced via electrospinning. SEM images elucidated that incorporation of AITC and AITC/β-CD-IC into polymer matrix did not affect the bead-free fiber morphology of PVA nanofibers. (1)H-NMR and headspace GC-MS analyses revealed that very low amount of AITC was remained in PVA/AITC-NF because of the rapid evaporation of AITC during the electrospinning process. Nevertheless, much higher amount of AITC was preserved in the PVA/AITC/β-CD-IC-NF due to the CD inclusion complexation. The sustained release of AITC from nanofibers was evaluated at 30°C, 50°C and 75°C via headspace GC-MS. When compared to PVA/AITC-NF, PVA/AITC/β-CD-IC-NF has shown higher antibacterial activity against Escherichia coli and Staphylococcus aureus due to the presence of higher amount of AITC in this sample which was preserved by CD-IC.
authors.affiliation: Institute of Materials Science & Nanotechnology, Bilkent University, Ankara 06800, Turkey; UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Aytac
authors.affiliation: Gazi University, Life Sciences Application and Research Center, Ankara 06830, Turkey.
authors.forename: Sema Y
authors.initials: SY
authors.lastname: Dogan
authors.affiliation: Gazi University, Life Sciences Application and Research Center, Ankara 06830, Turkey; Gazi University, Polatlı Science and Literature Faculty, Ankara 06900, Turkey.
authors.forename: Turgay
authors.initials: T
authors.lastname: Tekinay
authors.affiliation: Institute of Materials Science & Nanotechnology, Bilkent University, Ankara 06800, Turkey; UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey. Electronic address: tamer@unam.bilkent.edu.tr.
authors.forename: Tamer
authors.initials: T
authors.lastname: Uyar
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D065546:Drug Liberation; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D017879:Isothiocyanates / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D066244:Proton Magnetic Resonance Spectroscopy; D012996:Solutions; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D014961:X-Ray Diffraction; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D017879:Isothiocyanates; D012996:Solutions; D047392:beta-Cyclodextrins; C004471:allyl isothiocyanate; C031215:betadex
keywords: Allyl isothiocyanate; Antibacterial activity; Electrospinning; Nanofibers; Release; β-Cyclodextrin