title: Hepatic lipogenesis and a marker of hepatic lipid oxidation, predict postprandial responses of triglyceride-rich lipoproteins.
abstract: OBJECTIVE
  Postprandial hypertriglyceridemia is an important risk factor for cardiovascular disease. The mechanisms are still unclear. Here it was tested if hepatic de novo lipogenesis (DNL) and lipid oxidation influence the postprandial responses of triglyceride-rich lipoproteins (TRL) in humans.
  
  
  METHODS
  The contribution of hepatic DNL to hepatic TRL production was analyzed in 67 men and women with a moderate range of BMI after a fat-rich meal. Also, lipase activities, liver fat, and 3-OH-butyrate were quantitated as an indicator of β-oxidation. Lipoproteins and metabolic markers were measured in fasting and postprandial blood samples.
  
  
  RESULTS
  Postprandial DNL correlates with postprandial TG and apolipoprotein (apo) C-III responses in plasma and with TG, apoB48 and apoB100 responses in TRLs and their larger remnant particles. Fasting and 8-h postprandial DNL was inversely related to 3-OH-butyrate but not to liver fat content. Fasting apoC-III and 3-OH-butyrate, but not liver fat, independently predicted fasting DNL.
  
  
  CONCLUSIONS
  The fasting and 8-h postprandial rate of DNL was inversely associated with the hepatic lipid oxidation in humans. DNL contributes significantly to the TG content in TRLs but not to the amount of liver fat, suggesting that an imbalance between DNL and fat oxidation contributes to postprandial atherogenic dyslipidemia.
authors.affiliation: Department of Medicine, Cardiovascular Research Unit, Diabetes and Obesity Research Program, Heart and Lung Center, University of Helsinki, Finland; Division of Endocrinology, Helsinki University Central Hospital, University of Helsinki, Finland.
authors.forename: Niina
authors.initials: N
authors.lastname: Matikainen
authors.forename: Martin
authors.initials: M
authors.lastname: Adiels
authors.forename: Sanni
authors.initials: S
authors.lastname: Söderlund
authors.forename: Sanna
authors.initials: S
authors.lastname: Stennabb
authors.forename: Tytti
authors.initials: T
authors.lastname: Ahola
authors.forename: Antti
authors.initials: A
authors.lastname: Hakkarainen
authors.forename: Jan
authors.initials: J
authors.lastname: Borén
authors.forename: Marja-Riitta
authors.initials: MR
authors.lastname: Taskinen
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000378:metabolism; D000328:Adult; D053299:Apolipoprotein B-100 / Q000097:blood; D053283:Apolipoprotein B-48 / Q000097:blood; D053305:Apolipoprotein C-III / Q000097:blood; D005215:Fasting; D005260:Female; D006801:Humans; D015228:Hypertriglyceridemia / Q000378:metabolism; D050356:Lipid Metabolism; D050155:Lipogenesis; D008074:Lipoproteins / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D019518:Postprandial Period / Q000502:physiology; D014280:Triglycerides / Q000096:biosynthesis* / Q000097:blood
chemical_list: D053299:Apolipoprotein B-100; D053283:Apolipoprotein B-48; D053305:Apolipoprotein C-III; D008074:Lipoproteins; D014280:Triglycerides; C030087:lipoprotein triglyceride; D020155:3-Hydroxybutyric Acid