title: A general NMR-based strategy for the in situ characterization of sugar-nucleotide-dependent biosynthetic pathways.
abstract: A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively (13)C-labeled sugar nucleotides and inverse (13)C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.
authors.affiliation: Center for Pharmaceutical Research and Innovation, College of Pharmacy, University of Kentucky , 789 South Limestone Street, Lexington, Kentucky 40536, United States.
authors.forename: Shanteri
authors.initials: S
authors.lastname: Singh
authors.forename: Pauline
authors.initials: P
authors.lastname: Peltier-Pain
authors.forename: Marco
authors.initials: M
authors.lastname: Tonelli
authors.forename: Jon S
authors.initials: JS
authors.lastname: Thorson
mesh_terms: D053898:Biosynthetic Pathways; D002241:Carbohydrates / Q000096:biosynthesis / Q000737:chemistry*; D002247:Carbon Isotopes; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009711:Nucleotides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D002247:Carbon Isotopes; D009711:Nucleotides