title: Horseradish peroxidase-catalysed in situ-forming hydrogels for tissue-engineering applications.
abstract: In situ-forming hydrogels are an attractive class of implantable biomaterials that are used for biomedical applications. These injectable hydrogels are versatile and provide a convenient platform for delivering cells and drugs via minimally invasive surgery. Although several crosslinking methods for preparing in situ forming hydrogels have been developed over the past two decades, most hydrogels are not sufficiently versatile for use in a wide variety of tissue-engineering applications. In recent years, enzyme-catalysed crosslinking approaches have been emerged as a new approach for developing in situ-forming hydrogels. In particular, the horseradish peroxidase (HRP)-catalysed crosslinking approach has received increasing interest, due to its highly improved and tunable capacity to obtain hydrogels with desirable properties. The HRP-catalysed crosslinking reaction immediately occurs upon mixing phenol-rich polymers with HRP and hydrogen peroxide (H2O2) in aqueous media. Based on this unique gel-forming feature, recent studies have shown that various properties of formed hydrogels, such as gelation time, stiffness and degradation rate, can be easily manipulated by varying the concentrations of HRP and H2O2. In this review, we outline the versatile properties of HRP-catalysed in situ-forming hydrogels, with a brief introduction to the crosslinking mechanisms involved. In addition, the recent biomedical applications of HRP-catalysed in situ-forming hydrogels for tissue regeneration are described.
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Woncheon, Yeongtong, Suwon, Republic of Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Bae
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Woncheon, Yeongtong, Suwon, Republic of Korea.
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Woncheon, Yeongtong, Suwon, Republic of Korea.
authors.forename: Yunki
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Woncheon, Yeongtong, Suwon, Republic of Korea.
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Park
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002356:Cartilage / Q000473:pathology; D002384:Catalysis; D003432:Cross-Linking Reagents / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D051379:Mice; D019800:Phenol / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D051381:Rats; D012038:Regeneration; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020100:Hydrogels; D011108:Polymers; D019800:Phenol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase
keywords: enzymatic crosslinking; horseradish peroxidase; hydrogen peroxide; in situ-forming hydrogel; phenol-rich polymer; tissue regeneration