title: Polyphenols from the stems of Morus alba and their inhibitory activity against nitric oxide production by lipopolysaccharide-activated microglia.
abstract: Neuroinflammatory processes are involved in the pathogenesis of many neurodegenerative disorders. Microglial cells, the main immune cells of the central nervous system, represent a target of interest to search for naturally occurring anti-inflammatory products. In this study, we evaluated the anti-inflammatory properties of polyphenols obtained from the stems of Morus alba. This edible species, known as white mulberry, is frequently studied because of its traditional use in Asian medicine and its richness in different types of polyphenols, some of which are known to be phytoalexins. One new coumarin glycoside, isoscopoletin 6-(6-O-β-apiofuranosyl-β-glucopyranoside) (1) was mainly isolated by CPC (centrifugal partition chromatography) from this plant, together with seven known polyphenols (2-8). Their structures were established on the basis of spectroscopic analyses including extensive 2D NMR studies. The eight isolated compounds were evaluated for their inhibitory activities on nitric oxide (NO) production in lipopolysaccharide (LPS)-induced BV-2 microglial cells. The absence of cell toxicity is checked by a MTT assay.
authors.affiliation: Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Université Lille 2, F-59000 Lille, France; EA GRIIOT, EA4481, Laboratoire de pharmacognosie, UFR Pharmacie, Université Lille 2, F-59000 Lille, France; GESVAB EA 3675, ISVV, Université de Bordeaux, F-33882 Villenave d'Ornon Cedex, France. Electronic address: celine.riviere-3@univ-lille2.fr.
authors.forename: Céline
authors.initials: C
authors.lastname: Rivière
authors.affiliation: GESVAB EA 3675, ISVV, Université de Bordeaux, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Krisa
authors.affiliation: GESVAB EA 3675, ISVV, Université de Bordeaux, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Péchamat
authors.affiliation: GESVAB EA 3675, ISVV, Université de Bordeaux, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Merian
authors.initials: M
authors.lastname: Nassra
authors.affiliation: GESVAB EA 3675, ISVV, Université de Bordeaux, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Delaunay
authors.affiliation: Unité de recherche Œnologie, EA 4577, ISVV, Université de Bordeaux, F-33882 Villenave d'Ornon, France; USC 1366 Œnologie, Institut National de Recherche Agronomique, ISVV, F-33882 Villenave d'Ornon, France.
authors.forename: Axel
authors.initials: A
authors.lastname: Marchal
authors.affiliation: GESVAB EA 3675, ISVV, Université de Bordeaux, F-33882 Villenave d'Ornon Cedex, France; Jardin Botanique Universitaire de Talence, Université de Bordeaux, F-33400 Talence, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Badoc
authors.affiliation: GESVAB EA 3675, ISVV, Université de Bordeaux, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Waffo-Téguo
authors.affiliation: GESVAB EA 3675, ISVV, Université de Bordeaux, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mérillon
mesh_terms: D000818:Animals; D002460:Cell Line; D003374:Coumarins / Q000302:isolation & purification* / Q000494:pharmacology; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D015394:Molecular Structure; D031625:Morus / Q000737:chemistry*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry; D059808:Polyphenols / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D003374:Coumarins; D006027:Glycosides; D008070:Lipopolysaccharides; D059808:Polyphenols; D009569:Nitric Oxide
keywords: Coumarins; Dihydroflavonols; Flavanones; Inflammation; Microglia; Moraceae; Morus alba; Stilbenes