title: Relationship between permeability glycoprotein (P-gp) gene expression and enrofloxacin metabolism in Nile Tilapia.
abstract: The aim of this study was to analyze the influence of permeability glycoprotein (P-gp) gene expression on enrofloxacin (ENR) metabolism in aquatic animals. Nile Tilapia Oreochomis niloticus were fed different doses of ENR ranging from 0 to 80 mg/kg. The P-gp gene expression levels were determined by quantitative real-time PCR (qRT-PCR) at indicated time points after drug administration. Drug metabolism was determined by HPLC. The P-gp gene expression in liver and kidney was greatly enhanced 30 min after ENR administration at 40 mg/kg, peaked 3 h after drug administration, and then gradually decreased. Thirty minutes after a single oral administration of ENR (0, 20, 40, or 80 mg/kg), the P-gp gene expression increased in a dose-dependent manner. The P-gp gene expression levels in the kidney were significantly higher than those in the liver. Additionally, the metabolic rate of ENR in kidney was more rapid than that in liver. Furthermore, a close correlation was found between P-gp gene expression and ENR concentrations. These results suggest that P-gp may be involved in the ENR metabolism process in Nile Tilapia, providing a novel model for the potential utility of gene expression and drug metabolism studies in aquatic animals.
authors.affiliation: a National Pathogen Collection Center for Aquatic Animals , Shanghai Ocean University , 999 Hucheng Huan Road, Shanghai , 201306 , China.
authors.forename: Kun
authors.initials: K
authors.lastname: Hu
authors.forename: Gang
authors.initials: G
authors.lastname: Cheng
authors.forename: Haixin
authors.initials: H
authors.lastname: Zhang
authors.forename: Huicong
authors.initials: H
authors.lastname: Wang
authors.forename: Jiming
authors.initials: J
authors.lastname: Ruan
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Wenhong
authors.initials: W
authors.lastname: Fang
authors.forename: Xianle
authors.initials: X
authors.lastname: Yang
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000493:pharmacokinetics*; D023681:Cichlids / Q000378:metabolism*; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000378:metabolism / Q000493:pharmacokinetics*; D005786:Gene Expression Regulation / Q000502:physiology*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin