title: Rapid profiling of phenolic compounds of green and fermented Bergenia crassifolia L. leaves by UPLC-DAD-QqQ-MS and HPLC-DAD-ESI-QTOF-MS.
abstract: Bergenia crassifolia L., Saxifragaceae, is an evergreen perennial plant known in traditional medicine of Russia, Mongolia and China. Polyphenols are responsible for the number of pharmacological effects of Bergenia. UPLC-DAD-QqQ-MS and LC-DAD-ESI-QTOF-MS were used for the rapid profiling of phenolic compounds, mainly hydrolysable tannins. Green leaves consisted of 55% ellagitannins, 29% gallic acid derivatives and 11% flavonoids, with the remaining gallic acid, arbutin, bergenin and caffeoyl quinic acid. In fermented leaves, 31% of gallic acid was found, followed with 28% ellagitannins, 18% gallic acid derivatives and 18% flavonoids, with the remaining caffeoyl quinic acid, bergenin and arbutin. Tellimagrandin I, pedunculagin, caffeoyl quinic acid, monogalloyl quinic acid, 1-O-galloylglucose and 1,2,6-tri-O-galloylglucose were identified for the very first time.
authors.affiliation: a Department of Chemistry , University of Turku , Turku FIN-20014 , Finland.
authors.forename: Juha-Pekka
authors.initials: JP
authors.lastname: Salminen
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Shikov
authors.forename: Maarit
authors.initials: M
authors.lastname: Karonen
authors.forename: Olga N
authors.initials: ON
authors.lastname: Pozharitskaya
authors.forename: Jorma
authors.initials: J
authors.lastname: Kim
authors.forename: Valery G
authors.initials: VG
authors.lastname: Makarov
authors.forename: Raimo
authors.initials: R
authors.lastname: Hiltunen
authors.forename: Bertalan
authors.initials: B
authors.lastname: Galambosi
mesh_terms: D001104:Arbutin; D002681:China; D002851:Chromatography, High Pressure Liquid; D004610:Ellagic Acid / Q000032:analysis; D005285:Fermentation; D005419:Flavonoids / Q000032:analysis; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D047348:Hydrolyzable Tannins / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis* / Q000494:pharmacology; D031995:Saxifragaceae / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005419:Flavonoids; D005960:Glucosides; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; D059808:Polyphenols; C087111:6-O-galloylglucose; D004610:Ellagic Acid; C060474:glucogallin; D005707:Gallic Acid; C087926:tellimagrandin I; D001104:Arbutin
keywords: LC-DAD-ESI-QTOF-MS; UPLC-DAD-QqQ-MS; ellagitannins; gallic acid; hydrolysable tannins