title: Simultaneous determination of paeoniflorin, albiflorin, ferulic acid, tetrahydropalmatine, protopine, typhaneoside, senkyunolide I in Beagle dogs plasma by UPLC-MS/MS and its application to a pharmacokinetic study after Oral Administration of Shaofu Zhuyu Decoction.
abstract: In this present study, a sensitive and rapid UPLC-MS/MS method was developed for simultaneous quantification of paeoniflorin, albiflorin, ferulic acid, tetrahydropalmatine, protopine, typhaneoside and senkyunolide I in Beagle dog plasma after oral administration of the Shao-Fu-Zhu-Yu Decoction. Chloramphenicol and clarithromycin were used as internal standards. Plasma samples were processed by protein precipitation with methanol. The separation was performed on an Acquity BEH C18 column (100mm×2.1mm, 1.7μm) at a flow-rate of 0.4mL/min, using 0.1% formic acid-acetonitrile as mobile phase. Method validation was performed as per Food and Drug Administration guidelines and the results met the acceptance criteria. After validation, this method was successfully applied to a pharmacokinetic study. The results showed that the apparent plasma clearance of paeoniflorin, albiflorin, typhaneoside and senkyunolide I were significantly higher than others. Double peak was observed in plasma concentration curves of tetrahydropalmatine, the ferulic acid had a good absorption in Beagle dog plasma, and senkyunolide I was detected in plasma from the first blood sampling time (15min) and rapidly reached Tmax. The compound of typhaneoside has a low bioavailability according to the results.
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China; School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Huang
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China; School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China. Electronic address: sushulan1974@163.com.
authors.forename: Shulan
authors.initials: S
authors.lastname: Su
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China; School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Cui
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China; School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China; School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China. Electronic address: duanja@163.com.
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China; School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Guo
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China; School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Zhenhao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China; School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Erxin
authors.initials: E
authors.lastname: Shang
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China; School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Qian
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China; School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001572:Benzofurans / Q000097:blood; D053119:Benzophenanthridines / Q000097:blood; D001600:Berberine Alkaloids / Q000097:blood; D001952:Bridged-Ring Compounds / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000097:blood; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D005960:Glucosides / Q000097:blood; D006027:Glycosides / Q000097:blood; D039821:Monoterpenes / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001572:Benzofurans; D053119:Benzophenanthridines; D001600:Berberine Alkaloids; D001952:Bridged-Ring Compounds; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; D039821:Monoterpenes; C529850:Shao-Fu-Zhu-Yu; C051428:typhaneoside; C576743:senkyunolide I; C015423:peoniflorin; C014959:albiflorin; C014215:tetrahydropalmatine; C004999:ferulic acid; C009093:protopine
keywords: Beagle dog plasma; Pharmacokinetics; Shaofu Zhuyu decoction; UPLC–MS/MS