title: Nucleobase-based barbiturates: their protective effect against DNA damage induced by bleomycin-iron, antioxidant, and lymphocyte transformation assay.
abstract: A number of nucleobase-based barbiturates have been synthesized by combination of nucleic acid bases and heterocyclic amines and barbituric acid derivatives through green and efficient multicomponent route and one pot reaction. This approach was accomplished efficiently using aqueous medium to give the corresponding products in high yield. The newly synthesized compounds were characterized by spectral analysis (FT-IR, (1)H NMR, (13)C NMR, HMBC, and UV spectroscopy) and elemental analysis. Representative of all synthesized compounds was tested and evaluated for antioxidant, bleomycin-dependent DNA damage, and Lymphocyte Transformation studies. Compounds TBC > TBA > TBG showed highest lymphocyte transformation assay, TBC > TBA > BG showed inhibitory antioxidant activity using ABTS methods, and TBC > BPA > BAMT > TBA > 1, 3 -TBA manifested the best protective effect against DNA damage induced by bleomycin.
authors.affiliation: Department of Applied Chemistry, S.V. National Institute of Technology, Ichchhanath, Surat, Gujarat 395007, India.
authors.forename: Bhaveshkumar D
authors.initials: BD
authors.lastname: Dhorajiya
authors.affiliation: Department of Applied Chemistry, S.V. National Institute of Technology, Ichchhanath, Surat, Gujarat 395007, India.
authors.forename: Bharatkumar Z
authors.initials: BZ
authors.lastname: Dholakiya
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Ahmed S
authors.initials: AS
authors.lastname: Ibrahim
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Farid A
authors.initials: FA
authors.lastname: Badria
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001463:Barbiturates / Q000494:pharmacology*; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects*; D009696:Nucleic Acids / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001463:Barbiturates; D009696:Nucleic Acids; C023503:iron bleomycin; D001761:Bleomycin; C032232:barbituric acid