title: A facile approach for the polymer grafting of silica based on tandem reversible addition fragmentation chain transfer/click chemistry and its application in high performance liquid chromatography.
abstract: In this work, a tandem reversible addition fragmentation chain transfer (RAFT)/click chemistry method was developed to prepare amide-polystyrene-silica (NHCO-Psty-silica) stationary phase. Styrene was immobilized on amino-silica surface via an azide functionalized RAFT agent in a one-pot procedure. The resultant NHCO-Psty-silica column demonstrates better performance for shielding of residue silanols than traditional ODS column, which was ascertained by Engelhardt test (E test), Tanaka test (T test), Galushko test (G test), and Walters test (W test). Our results showed lower values of silanol activity calculated according to the formula in these standard tests for NHCO-Psty-silica column compared to the ODS column we tested. As a result, the NHCO-Psty-silica is suitable for the separation of basic compounds. The hydrophobic, anion exchanging and π-π interaction of the column toward analytes were also evaluated. Moreover, the NHCO-Psty-silica column also showed excellent stability with pure water as mobile phase.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Luojiashan, wucang, Wuhan 430072, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Luojiashan, wucang, Wuhan 430072, China.
authors.forename: Ming-Luan
authors.initials: ML
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Luojiashan, wucang, Wuhan 430072, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Luojiashan, wucang, Wuhan 430072, China.
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Shi
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Luojiashan, wucang, Wuhan 430072, China.
authors.forename: Bi-Feng
authors.initials: BF
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Luojiashan, wucang, Wuhan 430072, China. Electronic address: yqfeng@whu.edu.cn.
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
mesh_terms: D002851:Chromatography, High Pressure Liquid; D057930:Click Chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D011108:Polymers; D011137:Polystyrenes; D012821:Silanes; C082343:silanol; D012822:Silicon Dioxide
keywords: High performance liquid chromatography; NHCO-Psty–silica; Tandem RAFT/click chemistry