title: Comparison of the GenoType(®) MTBC Molecular Genetic Assay with culture methods in the diagnosis of tuberculosis.
abstract: BACKGROUND
  Clinical samples from 433 patients pre-diagnosed with tuberculosis in Konya, Turkey, were investigated prospectively to compare the GenoType(®) MTBC test (GenoType(®) MTBC) with conventional "gold standard" culture methods.
  
  
  METHODS
  Lowenstein Jensen (LJ) and Mycobacteria Growth Indicator Tube (MGIT)-960 culture methods and GenoType(®) MTBC were performed together.
  
  
  RESULTS
  Mycobacterium tuberculosis (M. tuberculosis) detection rates were 16.2% by culture methods, 15.4% by GenoType(®) MTBC, and 6% by acid-fast bacilli microscopy. The LJ or MGIT-960 with GenoType(®) MTBC detected M. tuberculosis in 12 samples each that were negative according to the other culture method alone. Among 70 M. tuberculosis-positive samples, detection rates were 37% (26/70) by microscopy and 82.8% (58/70) by LJ and MGIT-960, but 95.7% (67/70) by GenoType(®) MTBC.
  
  
  CONCLUSIONS
  GenoType(®) MTBC may be used as a beneficial adjunct test to culture methods for the detection of M. tuberculosis.
authors.affiliation: Department of Microbiology and Clinical Microbiology, Konya Education and Research Hospital, Konya, Turkey.
authors.forename: Muhammet Guzel
authors.initials: MG
authors.lastname: Kurtoglu
authors.affiliation: Department of Microbiology and Clinical Microbiology, Necmettin Erbakan University Meram Faculty of Medicine, Konya, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozdemir
authors.affiliation: Department of Microbiology and Clinical Microbiology, Konya Education and Research Hospital, Konya, Turkey.
authors.forename: Recep
authors.initials: R
authors.lastname: Kesli
authors.affiliation: Department of Microbiology and Clinical Microbiology, Necmettin Erbakan University Meram Faculty of Medicine, Konya, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Baysal
keywords: Mycobacterium tuberculosis; culture; genotype