title: Mitochondrial perturbation negatively affects auxin signaling.
abstract: Mitochondria are crucial players in the signaling and metabolic homeostasis of the plant cell. The molecular components that orchestrate the underlying processes, however, are largely unknown. Using a chemical biology approach, we exploited the responsiveness of Arabidopsis UDP-glucosyltransferase-encoding UGT74E2 towards mitochondrial perturbation in order to look for novel mechanisms regulating mitochondria-to-nucleus communication. The most potent inducers of UGT74E2 shared a (2-furyl)acrylate (FAA) substructure that negatively affected mitochondrial function and was identified before as an auxin transcriptional inhibitor. Based on these premises, we demonstrated that perturbed mitochondria negatively affect the auxin signaling machinery. Moreover, chemical perturbation of polar auxin transport and auxin biosynthesis was sufficient to induce mitochondrial retrograde markers and their transcript abundance was constitutively elevated in the absence of the auxin transcriptional activators ARF7 and ARF19.
authors.affiliation: a Department of Plant Systems Biology, VIB, Ghent University, B-9052 Gent, Belgium b Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium.
authors.forename: Pavel Ivanov
authors.initials: PI
authors.lastname: Kerchev
authors.affiliation: a Department of Plant Systems Biology, VIB, Ghent University, B-9052 Gent, Belgium b Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium.
authors.forename: Inge
authors.initials: I
authors.lastname: De Clercq
authors.affiliation: a Department of Plant Systems Biology, VIB, Ghent University, B-9052 Gent, Belgium b Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium.
authors.forename: Jordi
authors.initials: J
authors.lastname: Denecker
authors.forename: Per
authors.initials: P
authors.lastname: Mühlenbock
authors.affiliation: a Department of Plant Systems Biology, VIB, Ghent University, B-9052 Gent, Belgium b Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium.
authors.forename: Robert
authors.initials: R
authors.lastname: Kumpf
authors.affiliation: d VIB Compound Screening Facility, B-9052 Gent, Belgium.
authors.forename: Long
authors.initials: L
authors.lastname: Nguyen
authors.affiliation: a Department of Plant Systems Biology, VIB, Ghent University, B-9052 Gent, Belgium b Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium d VIB Compound Screening Facility, B-9052 Gent, Belgium.
authors.forename: Dominique
authors.initials: D
authors.lastname: Audenaert
authors.affiliation: a Department of Plant Systems Biology, VIB, Ghent University, B-9052 Gent, Belgium b Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Dejonghe
authors.affiliation: a Department of Plant Systems Biology, VIB, Ghent University, B-9052 Gent, Belgium b Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium frank.vanbreusegem@psb.vib-ugent.be.
authors.forename: Frank
authors.initials: F
authors.lastname: Van Breusegem
mesh_terms: D000179:Acrylates / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D007210:Indoleacetic Acids / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000187:drug effects / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000179:Acrylates; D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; C036658:acrylic acid
keywords: auxin signaling; mitochondrial perturbation; retrograde communication.