title: Cyanidin 3-O-glucoside reduces Helicobacter pylori VacA-induced cell death of gastric KATO III cells through inhibition of the SecA pathway.
abstract: Two key virulence factors of Helicobacter pylori are the secreted virulent proteins of vacuolating toxin A (VacA) and cytotoxin associated protein A (CagA) which lead to damages of gastric epithelial cells. We previously identified that the cyanidin 3-O-glucoside (C3G) inhibits the secretion of both VacA and CagA. In the current report, we show that C3G inhibits VacA secretion in a dose-dependent manner by inhibiting secretion system subunit protein A (SecA) synthesis. As SecA is involved in translocation of bacterial proteins, we predicted that inhibition of the SecA pathway by C3G should decrease H. pylori-induced cell death. To test this hypothesis, the human gastric cell line KATO III cells were co-cultured with H. pylori 60190 (VacA(+)/CagA(+)) and C3G. We found that C3G treatment caused a decrease in activation of the pro-apoptotic proteins caspase-3/-8 in H. pylori-infected cells leading to a decrease in cell death. Our data suggest that consumption of foods containing anthocyanin may be beneficial in reducing cell damage due to H. pylori infection.
authors.affiliation: 1. Department of Clinical Laboratory Science, Semyung University, Jaecheon 390-711, Republic of Korea;
authors.forename: Sa-Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: 2. Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju 220-710, Republic of Korea;
authors.forename: Hyunjun
authors.initials: H
authors.lastname: Woo
authors.affiliation: 2. Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju 220-710, Republic of Korea;
authors.forename: Min
authors.initials: M
authors.lastname: Park
authors.affiliation: 2. Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju 220-710, Republic of Korea;
authors.forename: Ki-Jong
authors.initials: KJ
authors.lastname: Rhee
authors.affiliation: 1. Department of Clinical Laboratory Science, Semyung University, Jaecheon 390-711, Republic of Korea;
authors.forename: Cheol
authors.initials: C
authors.lastname: Moon
authors.affiliation: 3. Department of Clinical Laboratory Science, Hyegeon College, Hongseong 350-702, Republic of Korea;
authors.forename: Dongsup
authors.initials: D
authors.lastname: Lee
authors.affiliation: 4. Department of Functional Crops, National Institute of Crop Science, Rural Development Administration, Miryang 627-803, Republic of Korea.
authors.forename: Woo Duck
authors.initials: WD
authors.lastname: Seo
authors.affiliation: 2. Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju 220-710, Republic of Korea;
authors.forename: Jong Bae
authors.initials: JB
authors.lastname: Kim
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000872:Anthocyanins / Q000008:administration & dosage*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D005753:Gastric Mucosa / Q000187:drug effects*; D005960:Glucosides / Q000008:administration & dosage*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000872:Anthocyanins; D001426:Bacterial Proteins; D005960:Glucosides; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; C074689:VacA protein, Helicobacter pylori; C462279:cyanidin-3-O-beta-glucopyranoside; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins
keywords: H. pylori; VacA secretion; cyanidin 3-O-glucoside