title: FGF-21 and skeletal remodeling during and after lactation in C57BL/6J mice.
abstract: Lactation is associated with significant alterations in both body composition and bone mass. Systemic and local skeletal factors such as receptor activator of nuclear factor κ-B ligand (RANKL), PTHrP, calcitonin, and estrogen are known to regulate bone remodeling during and after lactation. Fibroblast growth factor 21 (FGF-21) may function as an endocrine factor to regulate body composition changes during lactation by inducing gluconeogenesis and fatty acid oxidation. In this study, we hypothesized that the metabolic changes during lactation were due in part to increased circulating FGF-21, which in turn could accentuate bone loss. We longitudinally characterized body composition in C57BL/6J (B6) mice during (day 7 and day 21 of lactation) and after normal lactation (day 21 postlactation). At day 7 of lactation, areal bone density declined by 10% (P < .001), bone resorption increased (P < .0001), percent fat decreased by 20%, energy expenditure increased (P < .01), and markers of brown-like adipogenesis were suppressed in the inguinal depot and in preformed brown adipose tissue. At day 7 of lactation there was a 2.4-fold increase in serum FGF-21 vs baseline (P < .0001), a 8-fold increase in hepatic FGF-21 mRNA (P < .03), a 2-fold increase in undercarboxylated osteocalcin (Glu13 OCn) (P < .01), and enhanced insulin sensitivity. Recovery of total areal bone density was noted at day 21 of lactation, whereas the femoral trabecular bone volume fraction was still reduced (P < .01). Because FGF-21 levels rose rapidly at day 7 of lactation in B6 lactating mice, we next examined lactating mice with a deletion in the Fgf21 gene. Trabecular and cortical bone masses were maintained throughout lactation in FGF-21(-/-) mice, and pup growth was normal. Compared with lactating control mice, lactating FGF-21(-/-) mice exhibited an increase in bone formation, but no change in bone resorption. In conclusion, in addition to changes in calciotropic hormones, systemic FGF-21 plays a role in skeletal remodeling and changes in body composition during lactation in B6 mice.
authors.affiliation: Maine Medical Center Research Institute (S.B., P.T.L., V.D., C.J.R.), Scarborough, Maine 04074; Division of Endocrinology, University of Virginia (S.A.B.), Charlottesville, Virginia 22904; Division of Pharmacology (X.W., W.W., Y.W.), University of Texas Southwestern, Dallas, Texas 75390; Department of Orthopaedics and Rehabilitation (M.C.H.), Yale University School of Medicine, New Haven, Connecticut 06510; Metabolism, Endocrinology and Diabetes Division (O.M.), University of Michigan, Ann Arbor, Michigan 48105; Harvard Dental School (R.B., S.L.), Boston, Massachusetts 02115; Department of Genetics (G.K., M.F.), Columbia University College of Physicians and Surgeons, New York, New York 10032; Division of Endocrinology (C.S.K.), Memorial University, St. John's, Newfoundland, Canada A1B 3V6; and Division of Endocrinology (D.C.), University of North Carolina Chapel Hill, Chapel Hill, North Carolina 27599.
authors.forename: Sheila
authors.initials: S
authors.lastname: Bornstein
authors.forename: Sue A
authors.initials: SA
authors.lastname: Brown
authors.forename: Phuong T
authors.initials: PT
authors.lastname: Le
authors.forename: Xunde
authors.initials: X
authors.lastname: Wang
authors.forename: Victoria
authors.initials: V
authors.lastname: DeMambro
authors.forename: Mark C
authors.initials: MC
authors.lastname: Horowitz
authors.forename: Ormond
authors.initials: O
authors.lastname: MacDougald
authors.forename: Roland
authors.initials: R
authors.lastname: Baron
authors.forename: Sutada
authors.initials: S
authors.lastname: Lotinun
authors.forename: Gerard
authors.initials: G
authors.lastname: Karsenty
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Mathieu
authors.initials: M
authors.lastname: Ferron
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Kovacs
authors.forename: David
authors.initials: D
authors.lastname: Clemmons
authors.forename: Yihong
authors.initials: Y
authors.lastname: Wan
authors.forename: Clifford J
authors.initials: CJ
authors.lastname: Rosen
mesh_terms: D000818:Animals; D015519:Bone Density; D016723:Bone Remodeling; D001862:Bone Resorption; D001842:Bone and Bones / Q000378:metabolism; D005260:Female; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism*; D007774:Lactation; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice / Q000235:genetics / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D011247:Pregnancy
chemical_list: C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors