title: Production, purification and therapeutic potential of egg yolk antibodies for treating Trypanosoma evansi infection.
abstract: The use of avian antibodies has aroused interest in biomedical research due to the numerous advantages compared to mammal's antibodies. Our study aimed to produce and purify IgY immunoglobulins in order to use as an alternative therapy against Trypanosoma evansi. Every 14 days, four New Hampshire chickens were immunized with trypomastigotes of T. evansi, totaling five inoculations. Eggs were collected during 70 days and the extraction of IgY was performed by precipitation through the PEG-6000 method. Characterization and purification of IgY anti-T. evansi were carried out by SDS-PAGE and Western blot, where heavy and light chains were detected. The production of IgY was noted during the whole period, and the average production was 2.87 ± 0.14 at the end of this study. Sample's titration allowed the quantification of specific IgY anti-T. evansi, with antibodies produced showing high avidity indexes. The results indicated that T. evansi is able to generate an immune response in poultry, resulting in a production of specific antibodies. In vivo test showed that IgY treatment resulted in increase of prepatent period, longevity and survival of infected animals, when compared with the positive control, demonstrating an initial, but no curative, trypanocidal activity.
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil. Electronic address: sampaio.cris@gmail.com.
authors.forename: Luzia Cristina Lencioni
authors.initials: LC
authors.lastname: Sampaio
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil.
authors.forename: Matheus Dellaméa
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil.
authors.forename: Thirssa Helena
authors.initials: TH
authors.lastname: Grando
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil.
authors.forename: Lucas Trevisan
authors.initials: LT
authors.lastname: Gressler
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil.
authors.forename: Dianni de Menezes
authors.initials: Dde M
authors.lastname: Capeleto
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil.
authors.forename: Mariângela Facco
authors.initials: MF
authors.lastname: de Sa
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil.
authors.forename: Francielli Pantella Kuns
authors.initials: FP
authors.lastname: de Jesus
authors.affiliation: Biotechnology Center, Universidade Federal de Pelotas (UFPel), Brazil.
authors.forename: Alceu Gonçalves
authors.initials: AG
authors.lastname: dos Santos
authors.affiliation: Biotechnology Center, Universidade Federal de Pelotas (UFPel), Brazil.
authors.forename: Andreia Nobre
authors.initials: AN
authors.lastname: Anciuti
authors.affiliation: Biotechnology Center, Universidade Federal de Pelotas (UFPel), Brazil.
authors.forename: Karina
authors.initials: K
authors.lastname: Colonetti
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil.
authors.forename: Daniel Roulim
authors.initials: DR
authors.lastname: Stainki
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil. Electronic address: sgmonteiro@uol.com.br.
authors.forename: Silvia Gonzalez
authors.initials: SG
authors.lastname: Monteiro
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D002645:Chickens / Q000276:immunology*; D004530:Egg Yolk / Q000276:immunology*; D005260:Female; D014345:Trypanosoma / Q000276:immunology*; D014352:Trypanosomiasis / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D000913:Antibodies, Protozoan
keywords: Avian immunoglobulin; IgY; Immunotherapy; Trypanosomosis