title: Avian influenza virus RNA extraction.
abstract: The efficient extraction and purification of viral RNA is critical for down-stream molecular applications whether it is the sensitive and specific detection of virus in clinical samples, virus gene cloning and expression, or quantification of avian influenza (AI) virus by molecular methods from experimentally infected birds. Samples can generally be divided into two types; enriched (e.g. virus stocks) and clinical. Clinical type samples, which may be tissues or swab material, are the most difficult to process due to the complex sample composition and possibly low virus titers. In this chapter two well established procedures for the isolation of AI virus RNA from common clinical specimen types and enriched virus stocks for further molecular applications will be presented.
authors.affiliation: Exotic and Emerging Avian Viral Diseases Unit, Southeast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service, 934 College Station Rd., Athens, GA, USA, Erica.spackman@ars.usda.gov.
authors.forename: Erica
authors.initials: E
authors.lastname: Spackman
authors.forename: Scott A
authors.initials: SA
authors.lastname: Lee
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D001717:Birds / Q000821:virology*; D005591:Chemical Fractionation / Q000379:methods*; D002988:Cloaca / Q000821:virology; D004777:Environment; D005243:Feces / Q000821:virology; D006146:Guanidines / Q000737:chemistry; D009980:Influenza A virus / Q000235:genetics*; D059346:Magnets; D008863:Microspheres; D010636:Phenols / Q000737:chemistry; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D006146:Guanidines; D010636:Phenols; D012367:RNA, Viral; C411644:trizol