title: Antifibrotic activity of conjugates based on amphiphilic pluronic F68 and hydrophobic pluronic L31 with hyaluronate-endo-β-N-acetylhexosaminidase in pulmonary fibrosis.
abstract: Antifibrotic activity of intranasally administered conjugates of pluronics L31 and F68 with hyaluronate-endo-β-N-acetylhexosaminidase was studied in C57Bl/6 mice under conditions of single and repeated bleomycin-induced injury to the alveolar epithelium. Conjugates were prepared using the technique of protein immobilization with ionizing radiation. We demonstrate that in cases of single and repeated injuries to the alveolar epithelium, the conjugates administered during phases of inflammation or deposition of fibrotic masses prevent the development of pulmonary fibrosis. The conjugates demonstrated more pronounced antifibrotic activity than hyaluronate-endo-β-N-acetylhexosaminidase. The conjugate based on hydrophobic pluronic L31 showed higher effectiveness in comparison with the conjugate based on amphiphilic pluronic F68.
authors.affiliation: Department of Pathophysiology and Regenerative Medicine Research Institute of Pharmacology, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk, Russia.
authors.forename: E G
authors.initials: EG
authors.lastname: Skurikhin
authors.forename: A M
authors.initials: AM
authors.lastname: Reztsova
authors.forename: N N
authors.initials: NN
authors.lastname: Ermakova
authors.forename: V A
authors.initials: VA
authors.lastname: Krupin
authors.forename: O V
authors.initials: OV
authors.lastname: Pershina
authors.forename: E S
authors.initials: ES
authors.lastname: Khmelevskaya
authors.forename: A V
authors.initials: AV
authors.lastname: Artamonov
authors.forename: A A
authors.initials: AA
authors.lastname: Bekarev
authors.forename: P G
authors.initials: PG
authors.lastname: Madonov
authors.forename: D N
authors.initials: DN
authors.lastname: Kinsht
authors.forename: D V
authors.initials: DV
authors.lastname: Reykhard
authors.forename: V E
authors.initials: VE
authors.lastname: Goldberg
authors.forename: A M
authors.initials: AM
authors.lastname: Dygai
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001761:Bleomycin; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000494:pharmacology*; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D008810:Mice, Inbred C57BL; D020442:Poloxamer / Q000737:chemistry*; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D011839:Radiation, Ionizing; D001619:beta-N-Acetylhexosaminidases / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D004800:Enzymes, Immobilized; D005343:Fibrinolytic Agents; D020442:Poloxamer; D001761:Bleomycin; D006820:Hyaluronic Acid; D001619:beta-N-Acetylhexosaminidases