title: Single nucleotide polymorphism and haplotype effects associated with somatic cell score in German Holstein cattle.
abstract: BACKGROUND
  To better understand the genetic determination of udder health, we performed a genome-wide association study (GWAS) on a population of 2354 German Holstein bulls for which daughter yield deviations (DYD) for somatic cell score (SCS) were available. For this study, we used genetic information of 44 576 informative single nucleotide polymorphisms (SNPs) and 11 725 inferred haplotype blocks.
  
  
  RESULTS
  When accounting for the sub-structure of the analyzed population, 16 SNPs and 10 haplotypes in six genomic regions were significant at the Bonferroni threshold of P ≤ 1.14 × 10-6. The size of the identified regions ranged from 0.05 to 5.62 Mb. Genomic regions on chromosomes 5, 6, 18 and 19 coincided with known QTL affecting SCS, while additional genomic regions were found on chromosomes 13 and X. Of particular interest is the region on chromosome 6 between 85 and 88 Mb, where QTL for mastitis traits and significant SNPs for SCS in different Holstein populations coincide with our results. In all identified regions, except for the region on chromosome X, significant SNPs were present in significant haplotypes. The minor alleles of identified SNPs on chromosomes 18 and 19, and the major alleles of SNPs on chromosomes 6 and X were favorable for a lower SCS. Differences in somatic cell count (SCC) between alternative SNP alleles reached 14 000 cells/mL.
  
  
  CONCLUSIONS
  The results support the polygenic nature of the genetic determination of SCS, confirm the importance of previously reported QTL, and provide evidence for the segregation of additional QTL for SCS in Holstein cattle. The small size of the regions identified here will facilitate the search for causal genetic variations that affect gene functions.
authors.forename: Hamdy
authors.initials: H
authors.lastname: Abdel-Shafy
authors.forename: Ralf H
authors.initials: RH
authors.lastname: Bortfeldt
authors.forename: Jens
authors.initials: J
authors.lastname: Tetens
authors.affiliation: Department for Crop and Animal Sciences, Humboldt-Universität zu Berlin, Berlin, Germany. gudrun.brockmann@agrar.hu-berlin.de.
authors.forename: Gudrun A
authors.initials: GA
authors.lastname: Brockmann
mesh_terms: D000818:Animals; D002417:Cattle / Q000145:classification* / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary; D002875:Chromosomes / Q000235:genetics; D056726:Genetic Association Studies / Q000662:veterinary*; D023281:Genomics; D005838:Genotype; D006239:Haplotypes; D008297:Male; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci