title: Release behavior and toxicity profiles towards leukemia (WEHI-3B) cell lines of 6-mercaptopurine-PEG-coated magnetite nanoparticles delivery system.
abstract: The coating of an active drug, 6-mercaptopurine, into the iron oxide nanoparticles-polyethylene glycol (FNPs-PEG) in order to form a new nanocomposite, FPEGMP-2, was accomplished using coprecipitation technique. The resulting nanosized with a narrow size distribution magnetic polymeric particles show the superparamagnetic properties with 38.6 emu/g saturation magnetization at room temperature. Fourier transform infrared spectroscopy and the thermal analysis study supported the formation of the nanocomposite and the enhancement of thermal stability in the resulting nanocomposite comparing with its counterpart in free state. The loading of 6-mercaptopurine (MP) in the FPEGMP-2 nanocomposite was estimated to be about 5.6% and the kinetic experimental data properly correlated with the pseudo-second order model. Also, the release of MP from the FPEGMP-2 nanocomposite shows the sustained release manner which is remarkably lower in phosphate buffered solution at pH 7.4 than pH 4.8, due to different release mechanism. The maximum percentage release of MP from the nanocomposite reached about 60% and 97% within about 92 and 74 hours when exposed to pH 7.4 and 4.8, respectively.
authors.affiliation: Materials Synthesis and Characterization Laboratory (MSCL), Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Dena
authors.identifier: 0000-0001-5414-9204
authors.initials: D
authors.lastname: Dorniani
authors.affiliation: Vaccines and Immunotherapeutics Laboratory (IBS), Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Aminu Umar
authors.initials: AU
authors.lastname: Kura
authors.affiliation: Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia ; Faculty of Pharmacy, Isra University, P.O. Box 22, Amman 11622, Jordan.
authors.forename: Samer Hasan
authors.initials: SH
authors.lastname: Hussein-Al-Ali
authors.affiliation: Materials Synthesis and Characterization Laboratory (MSCL), Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mohd Zobir
authors.initials: MZ
authors.lastname: bin Hussein
authors.affiliation: Vaccines and Immunotherapeutics Laboratory (IBS), Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Sharida
authors.initials: S
authors.lastname: Fakurazi
authors.affiliation: Physics Department, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Abdul Halim
authors.initials: AH
authors.lastname: Shaari
authors.affiliation: Vaccines and Immunotherapeutics Laboratory (IBS), Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia ; Chemical Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Zalinah
authors.initials: Z
authors.lastname: Ahmad
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods*; D007938:Leukemia; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D015122:Mercaptopurine / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D058185:Magnetite Nanoparticles; D011092:Polyethylene Glycols; D015122:Mercaptopurine