title: Production of corticosteroid hormones in vitro by adrenals in rats with streptozotocin-induced diabetes.
abstract: We studied baseline and ACTH-stimulated in vitro production of corticosteroids by rat adrenals. Production of the basic corticosteroids pregnenolone (early precursor in corticosteroid synthesis), progesterone (intermediate precursor in synthesis of gluco- and mineralocorticoid hormones), and corticosterone (major glucocorticoid hormone in rodents) in animals with streptozotocin-induced diabetes was enhanced by 1.8-2.0 times in comparison with the control animals. Addition of ACTH to the incubation medium stimulated pregnenolone production by the adrenals equally in the control and experimental (diabetic) groups, while the increase in corticosterone production was less pronounced in the experimental group. Stimulation of corticosterone production in response to ACTH after saturation of the incubation medium with pregnenolone was also less pronounced in diabetic rats.
authors.affiliation: Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia, labend@soramn.ru.
authors.forename: N V
authors.initials: NV
authors.lastname: Kuznetsova
authors.forename: N A
authors.initials: NA
authors.lastname: Pal'chikova
authors.forename: O I
authors.initials: OI
authors.lastname: Kuzminova
authors.forename: V G
authors.initials: VG
authors.lastname: Selyatitskaya
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D003345:Corticosterone / Q000096:biosynthesis* / Q000097:blood / Q000378:metabolism / Q000652:urine; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000652:urine*; D008297:Male; D009924:Organ Culture Techniques; D009929:Organ Size; D011284:Pregnenolone / Q000096:biosynthesis* / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis* / Q000378:metabolism / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
chemical_list: D011374:Progesterone; D013311:Streptozocin; D011284:Pregnenolone; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone