title: Rho GTPases, phosphoinositides, and actin: a tripartite framework for efficient vesicular trafficking.
abstract: Rho GTPases are well known regulators of the actin cytoskeleton that act by binding and activating actin nucleators. They are therefore involved in many actin-based processes, including cell migration, cell polarity, and membrane trafficking. With the identification of phosphoinositide kinases and phosphatases as potential binding partners or effectors, Rho GTPases also appear to participate in the regulation of phosphoinositide metabolism. Since both actin dynamics and phosphoinositide turnover affect the efficiency and the fidelity of vesicle transport between cell compartments, Rho GTPases have emerged as critical players in membrane trafficking. Rho GTPase activity, actin remodeling, and phosphoinositide metabolism need to be coordinated in both space and time to ensure the progression of vesicles along membrane trafficking pathways. Although most molecular pathways are still unclear, in this review, we will highlight recent advances made in our understanding of how Rho-dependent signaling pathways organize actin dynamics and phosphoinositides and how phosphoinositides potentially provide negative feedback to Rho GTPases during endocytosis, exocytosis and membrane exchange between intracellular compartments.
authors.affiliation: CNRS UPR 3212; Institut des Neurosciences Cellulaires et Intégratives; Université de Strasbourg; Strasbourg, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Croisé
authors.affiliation: CNRS UPR 3212; Institut des Neurosciences Cellulaires et Intégratives; Université de Strasbourg; Strasbourg, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Estay-Ahumada
authors.affiliation: CNRS UPR 3212; Institut des Neurosciences Cellulaires et Intégratives; Université de Strasbourg; Strasbourg, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Gasman
authors.affiliation: CNRS UPR 3212; Institut des Neurosciences Cellulaires et Intégratives; Université de Strasbourg; Strasbourg, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Ory
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002966:Clathrin / Q000378:metabolism; D004705:Endocytosis; D005089:Exocytosis; D006801:Humans; D010587:Phagocytosis; D010716:Phosphatidylinositols / Q000378:metabolism*; D021381:Protein Transport; D015398:Signal Transduction; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D000199:Actins; D002966:Clathrin; D010716:Phosphatidylinositols; D020741:rho GTP-Binding Proteins
keywords: Rho GTPases; RhoGAP; RhoGEF; actin; endocytosis; exocytosis; phospholipid signaling