title: The crystal structure of pyrimidine/thiamin biosynthesis precursor-like domain-containing protein CAE31940 from proteobacterium Bordetella bronchiseptica RB50, and evolutionary insight into the NMT1/THI5 family.
abstract: We report a 2.0 Å structure of the CAE31940 protein, a proteobacterial NMT1/THI5-like domain-containing protein. We also discuss the primary and tertiary structure similarity with its homologs. The highly conserved FGGXMP motif was identified in CAE31940, which corresponds to the GCCCX motif located in the vicinity of the active center characteristic for THi5-like proteins found in yeast. This suggests that the FGGXMP motif may be a unique hallmark of proteobacterial NMT1/THI5-like proteins.
authors.affiliation: Department of Molecular Physiology and Biological Physics, University of Virginia, 1340 Jefferson Park Avenue, Charlottesville, VA, 22908, USA.
authors.forename: Jacek
authors.initials: J
authors.lastname: Bajor
authors.forename: Karolina L
authors.initials: KL
authors.lastname: Tkaczuk
authors.forename: Maksymilian
authors.initials: M
authors.lastname: Chruszcz
authors.forename: Hutton
authors.initials: H
authors.lastname: Chapman
authors.forename: Olga
authors.initials: O
authors.lastname: Kagan
authors.forename: Alexei
authors.initials: A
authors.lastname: Savchenko
authors.forename: Wladek
authors.initials: W
authors.lastname: Minor
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D016950:Bordetella bronchiseptica; D018360:Crystallography, X-Ray; D019143:Evolution, Molecular; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins