title: Preparation and characterization of PHBV microsphere/45S5 bioactive glass composite scaffolds with vancomycin releasing function.
abstract: PHBV microsphere/45S5 bioactive glass (BG) composite scaffolds with drug release function were developed for bone tissue engineering. BG-based glass-ceramic scaffolds with high porosity (94%) and interconnected pore structure prepared by foam replication method were coated with PHBV microspheres (nominal diameter=3.5 μm) produced by water-in-oil-in-water double emulsion solvent evaporation method. A homogeneous microsphere coating throughout the porous structure of scaffolds was obtained by a simple dip coating method, using the slurry of PHBV microspheres in hexane. Compressive strength tests showed that the microsphere coating slightly improved the mechanical properties of the scaffolds. It was confirmed that the microsphere coating did not inhibit the bioactivity of the scaffolds in SBF. Hydroxyapatite crystals homogeneously grew not only on the struts of the scaffolds but also on the surface of microspheres within 7 days of immersion in SBF. Vancomycin was successfully encapsulated into the PHBV microspheres. The encapsulated vancomycin was released with a dual release profile involving a relatively low initial burst release (21%) and a sustained release (1 month), which is favorable compared to the high initial burst release (77%) and short release period (4 days) measured on uncoated scaffolds. The developed bioactive composite scaffold with drug delivery function has thus the potential to be used advantageously in bone tissue engineering.
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 Erlangen, Germany.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Martensstrasse 7, 91058 Erlangen, Germany.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 Erlangen, Germany.
authors.forename: Ranjana
authors.initials: R
authors.lastname: Rai
authors.affiliation: Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Martensstrasse 7, 91058 Erlangen, Germany.
authors.forename: Judith A
authors.initials: JA
authors.lastname: Roether
authors.affiliation: Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Martensstrasse 7, 91058 Erlangen, Germany.
authors.forename: Dirk W
authors.initials: DW
authors.lastname: Schubert
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 Erlangen, Germany. Electronic address: aldo.boccaccini@ww.uni-erlangen.de.
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D002516:Ceramics / Q000737:chemistry*; D019245:Compressive Strength; D004337:Drug Carriers / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D008863:Microspheres; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties; D013997:Time Factors; D014640:Vancomycin / Q000737:chemistry* / Q000378:metabolism; D000094122:Polyhydroxybutyrates
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D011091:Polyesters; D014640:Vancomycin; C425330:bioactive glass 45S5; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: Bioglass; PHBV; drug delivery; scaffold; tissue engineering; vancomycin