title: Concentration of enteric viruses from tap water using an anion exchange resin-based method.
abstract: Detecting low concentrations of enteric viruses in water is needed for public health-related monitoring and control purposes. Thus, there is a need for sensitive, rapid and cost effective enteric viral concentration methods compatible with downstream molecular detection. Here, a virus concentration method based on adsorption of the virus to an anion exchange resin and direct isolation of nucleic acids is presented. Ten liter samples of tap water spiked with different concentrations (10-10,000 TCID50/10 L) of human adenovirus 40 (HAdV-40), hepatitis A virus (HAV) or rotavirus (RV) were concentrated and detected by real time PCR or real time RT-PCR. This method improved viral detection compared to direct testing of spiked water samples where the ΔCt was 12.1 for AdV-40 and 4.3 for HAV. Direct detection of RV in water was only possible for one of the three replicates tested (Ct of 37), but RV detection was improved using the resin method (all replicates tested positive with an average Ct of 30, n=3). The limit of detection of the method was 10 TCID50/10 L for HAdV-40 and HAV, and 100 TCID50/10 L of water for RV. These results compare favorably with detection limits reported for more expensive and laborious methods.
authors.affiliation: Department of Animal Sciences, Colorado State University, Fort Collins, CO, USA. Electronic address: almperez@rams.colostate.edu.
authors.forename: A
authors.initials: A
authors.lastname: Pérez-Méndez
authors.affiliation: Department of Animal Science, University of Wyoming, Laramie, WY, USA. Electronic address: jchandl3@uwyo.edu.
authors.forename: J C
authors.initials: JC
authors.lastname: Chandler
authors.affiliation: Department of Animal Science, University of Wyoming, Laramie, WY, USA. Electronic address: bbisha@uwyo.edu.
authors.forename: B
authors.initials: B
authors.lastname: Bisha
authors.affiliation: Department of Food Science and Agricultural Chemistry, McGill University, Ste. Anne de Bellevue, Quebec, Canada. Electronic address: lawrence.goodridge@mcgill.ca.
authors.forename: L D
authors.initials: LD
authors.lastname: Goodridge
mesh_terms: D000260:Adenoviruses, Human / Q000302:isolation & purification*; D000837:Anion Exchange Resins; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D060766:Drinking Water / Q000821:virology*; D030041:Hepatitis A virus / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D000837:Anion Exchange Resins; D004279:DNA, Viral; D060766:Drinking Water; D012367:RNA, Viral
keywords: Adenovirus; Anion exchange resin; Enteric viruses; Hepatitis A virus; Rotavirus; Virus concentration methods