title: Activation of protease-activated receptor-2 disrupts vaginal epithelial barrier function.
abstract: The epithelial cell barrier function is a critical factor in the maintenance of the homeostasis of the vaginal mucosa. This study elucidates one of the mast cell-derived chemical mediators, tryptase, on compromising the vaginal epithelial barrier function. The results showed that human vaginal cell line, VK2, express PAR2. Activation of PAR2 increases the expression of ADAM10 in VK2 cells, interferes with the endosome/lysosome fusion, and compromises the epithelial barrier function. We conclude that the activation of PAR2 on VK2 cells increases the expression of ADAM10, and compromises the VK2 monolayer barrier function.
authors.affiliation: Department of Obstetrics and Gynecology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Chong
authors.initials: C
authors.lastname: Feng
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.forename: Jiyuan
authors.initials: J
authors.lastname: Zhang
authors.forename: Ronghua
authors.initials: R
authors.lastname: Zan
authors.forename: Guizhi
authors.initials: G
authors.lastname: Zheng
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Yang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhai
mesh_terms: D051722:ADAM Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000072197:ADAM10 Protein; D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002463:Cell Membrane Permeability; D011992:Endosomes / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D044464:Receptor, PAR-2 / Q000378:metabolism*; D053802:Tryptases / Q000378:metabolism; D014621:Vagina / Q000166:cytology
chemical_list: D008565:Membrane Proteins; D034741:RNA, Small Interfering; D044464:Receptor, PAR-2; D053829:Amyloid Precursor Protein Secretases; D053802:Tryptases; D051722:ADAM Proteins; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human
keywords: barrier function; metalloproteinase domain-containing protein 10; protease-activated receptor 2; tryptase; vaginal epithelial cell