title: Expression of Transthyretin during bovine myogenic satellite cell differentiation.
abstract: Adult myogenesis responsible for the maintenance and repair of muscle tissue is mainly under the control of myogenic regulatory factors (MRFs) and a few other genes. Transthyretin gene (TTR), codes for a carrier protein for thyroxin (T4) and retinol binding protein bound with retinol in blood plasma, plays a critical role during the early stages of myogenesis. Herein, we investigated the relationship of TTR with other muscle-specific genes and report their expression in muscle satellite cells (MSCs), and increased messenger RNA (mRNA) and protein expression of TTR during MSCs differentiation. Silencing of TTR resulted in decreased myotube formation and decreased expression of myosin light chain (MYL2), myosin heavy chain 3 (MYH3), matrix gla protein (MGP), and voltage-dependent L type calcium channel (Cav1.1) genes. Increased mRNA expression observed in TTR and other myogenic genes with the addition of T4 decreased significantly following TTR knockdown, indicating the critical role of TTR in T4 transportation. Similarly, decreased expression of MGP and Cav1.1 following TTR knockdown signifies the dual role of TTR in controlling muscle myogenesis via regulation of T4 and calcium channel. Our computational and experimental evidences indicate that TTR has a relationship with MRFs and may act on calcium channel and related genes.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.
authors.forename: Smritee
authors.initials: S
authors.lastname: Pokharel
authors.forename: Majid Rasool
authors.initials: MR
authors.lastname: Kamli
authors.forename: Bilal Ahmad
authors.initials: BA
authors.lastname: Mir
authors.forename: Adeel
authors.initials: A
authors.lastname: Malik
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Lee
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D024510:Muscle Development / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000502:physiology; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D011228:Prealbumin / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D032496:Satellite Cells, Skeletal Muscle / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013974:Thyroxine / Q000494:pharmacology
chemical_list: D011228:Prealbumin; D012333:RNA, Messenger; D013974:Thyroxine