title: In vitro evaluation of the enzymatic activity profile of non-albicans Candida species isolated from patients with oral candidiasis with or without diabetes.
abstract: OBJECTIVE
  To evaluate the expression of phospholipase (PL) and secreted aspartyl proteinase (SAP) by Candida glabrata and C tropicalis obtained from the denture biofilms of healthy participants (16 isolates), patients with oral candidiasis with diabetes (10 isolates), and patients with oral candidiasis without diabetes (25 isolates).
  
  
  METHODS
  After incubation, the supernatants and pellets of the isolates were used for the enzymatic assays and quantification of colony-forming units (CFU), respectively. Colorimetric tests were used with phosphatidylcholine as a substrate for PL and azocasein as a substrate for SAP, and the absorbances of the samples were measured. Enzymatic rates were calculated, and values were normalized by CFU. Results were analyzed with factorial analyses of variance (α = .05).
  
  
  RESULTS
  C tropicalis and C glabrata were proteolytic and phospholipolytic. The clinical sources of isolates had no significant effect on the enzymatic activities (P > .05). C tropicalis had significantly higher enzymatic activity for both PL and SAP (P < .001) than did C glabrata.
  
  
  CONCLUSIONS
  C tropicalis isolates produced significantly higher amounts of both enzymes than did the C glabrata isolates.
authors.affiliation: Substitute Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
authors.forename: Paula Volpato
authors.initials: PV
authors.lastname: Sanitá
authors.affiliation: PhD Student, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
authors.forename: Chaiene Evelin
authors.initials: CE
authors.lastname: Zago
authors.affiliation: Assistant Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
authors.forename: Ewerton Garcia de Oliveira
authors.initials: EG
authors.lastname: Mima
authors.affiliation: Associate Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Pavarina
authors.affiliation: Assistant Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
authors.forename: Janaina Habib
authors.initials: JH
authors.lastname: Jorge
authors.affiliation: Full Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
authors.forename: Ana Lúcia
authors.initials: AL
authors.lastname: Machado
authors.affiliation: Full Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil. Electronic address: vergani@foar.unesp.br.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Vergani
mesh_terms: D000368:Aged; D002175:Candida / Q000201:enzymology* / Q000302:isolation & purification*; D002180:Candidiasis, Oral / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D048909:Diabetes Complications / Q000382:microbiology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008828:Microbiological Techniques; D010447:Peptide Hydrolases / Q000032:analysis; D010740:Phospholipases / Q000032:analysis
chemical_list: D003470:Culture Media; D010740:Phospholipases; D010447:Peptide Hydrolases