title: Biocompatible core-shell electrospun nanofibers as potential application for chemotherapy against ovary cancer.
abstract: Polyvinyl alcohol/chitosan (PVA/CS) core-shell nanofibers are successfully fabricated by a simple coaxial electrospinning method, in which PVA forms the core layer and CS forms the shell layer. With the change of the feed ratio between PVA and CS, the surface morphology and the microstructures of the nanofibers are largely changed. The as-prepared core-shell fibers can be used as a carrier for doxorubicin (DOX) delivery. FT-IR analysis demonstrates that hydrogen bond between CS and PVA chains forms. The results of in vitro cytotoxicity test indicate that the core-shell fibers are completely biocompatible and the free DOX shows higher cytotoxicity than the DOX loaded nanofibers. The standing PVA/CS core-shell fibers remarkably promote the attachment, proliferation and spreading of human ovary cancer cells (SKOV3). Via observing by confocal laser scanning microscopy (CLSM), the DOX released from the fibers can be delivered into SKOV3 cell nucleus, which is significant for the future tumor therapy. And, the as-prepared fibers exhibit controlled release for loaded DOX via adjusting the feed ratio between PVA and CS, and the DOX loaded nanofibers are quite effective in prohibiting the SKOV3 ovary cells attachment and proliferation, which are potential for chemotherapy of ovary cancer.
authors.affiliation: College of Material Science and Engineering, Qiqihar University, Qiqihar 161006, PR China.
authors.forename: Eryun
authors.initials: E
authors.lastname: Yan
authors.affiliation: College of Material Science and Engineering, Qiqihar University, Qiqihar 161006, PR China.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Key Laboratory of Polymer Functional Materials, Heilongjiang University, Harbin 150080, PR China.
authors.forename: Zhiyao
authors.initials: Z
authors.lastname: Sun
authors.affiliation: College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, PR China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Material Science and Engineering, Qiqihar University, Qiqihar 161006, PR China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Hao
authors.affiliation: College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, PR China.
authors.forename: Shichun
authors.initials: S
authors.lastname: Pei
authors.affiliation: Key Laboratory of Polymer Functional Materials, Heilongjiang University, Harbin 150080, PR China. Electronic address: wangc_93@163.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Polymer Functional Materials, Heilongjiang University, Harbin 150080, PR China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Sun
authors.affiliation: College of Material Science and Engineering, Qiqihar University, Qiqihar 161006, PR China. Electronic address: zhdqing@163.com.
authors.forename: Deqing
authors.initials: D
authors.lastname: Zhang
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D006801:Humans; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010051:Ovarian Neoplasms / Q000188:drug therapy; D011142:Polyvinyl Alcohol / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D004317:Doxorubicin; D011142:Polyvinyl Alcohol; D048271:Chitosan
keywords: Chemotherapy; Chitosan; Coaxial electrospinning; Nanofibers; Polyvinyl alcohol