title: Detection of influenza A antibodies in avian serum samples by ELISA.
abstract: ELISA assays are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive, they provide a ready-to-use assay with good quality control. Various sample types can be processed for ELISA: serum, plasma, egg yolk, blood collected on filter paper. Quality samples are critical to accurate results. The basics of AIV antibody ELISA, sample processing, results interpretation, and troubleshooting are discussed.
authors.affiliation: Georgia Poultry Laboratory Network, Oak wood, GA, USA.
authors.forename: Len
authors.initials: L
authors.lastname: Chappell
authors.forename: Mary Lea
authors.initials: ML
authors.lastname: Killian
authors.forename: Erica
authors.initials: E
authors.lastname: Spackman
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001717:Birds / Q000097:blood* / Q000821:virology*; D001800:Blood Specimen Collection; D004530:Egg Yolk / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D009980:Influenza A virus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral