title: In vivo study of targeted nanomedicine delivery into Langerhans cells by multiphoton laser scanning microscopy.
abstract: Epidermal Langerhans cells (LCs) function as professional antigen-presenting cells of the skin. We investigated the LC-targeting properties of a special mannose-moiety-coated pathogen-like synthetic nanomedicine DermaVir (DV), which is capable to express antigens to induce immune responses and kill HIV-infected cells. Our aim was to use multiphoton laser microscopy (MLM) in vivo in order to visualize the uptake of Alexa-labelled DV (AF546-DV) by LCs. Knock-in mice expressing enhanced green fluorescent protein (eGFP) under the control of the langerin gene (CD207) were used to visualize LCs. After 1 h, AF546-DV penetrated the epidermis and entered the eGFP-LCs. The AF546-DV signal was equally distributed inside the LCs. After 9 h, we observed AF546-DV signal accumulation that occurred mainly at the cell body. We demonstrated in live animals that LCs picked up and accumulated the nanoparticles in the cell body.
authors.affiliation: Institute for Solid State Physics and Optics of Wigner RCP, Budapest, Hungary; R&D Ultrafast Lasers Ltd, Budapest, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Kolonics
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Csiszovszki
authors.forename: Enikő R
authors.initials: ER
authors.lastname: Tőke
authors.forename: Orsolya
authors.initials: O
authors.lastname: Lőrincz
authors.forename: Dóra
authors.initials: D
authors.lastname: Haluszka
authors.forename: Róbert
authors.initials: R
authors.lastname: Szipőcs
mesh_terms: D016915:AIDS Vaccines / Q000493:pharmacokinetics*; D000818:Animals; D001692:Biological Transport; D049452:Green Fluorescent Proteins / Q000235:genetics; D007801:Langerhans Cells / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D036641:Microscopy, Fluorescence, Multiphoton; D023421:Models, Animal; D050997:Nanomedicine / Q000379:methods*; D053758:Nanoparticles
chemical_list: D016915:AIDS Vaccines; C501366:DermaVir; D049452:Green Fluorescent Proteins
keywords: Langerhans cells; eGFP-Langerin knock-in mice; in vivo; multiphoton laser microscopy; nanomedicine formulation