title: Improving coenzyme Q8 production in Escherichia coli employing multiple strategies.
abstract: Coenzyme Q (CoQ) is a medically valuable compound and a high yielding strain for CoQ will have several benefits for the industrial production of CoQ. To increase the CoQ(8) content of E. coli, we blocked the pathway for the synthesis of menaquinone by deleting the menA gene. The blocking of menaquinone pathway increased the CoQ(8) content by 81 % in E. coli (ΔmenA). To study the CoQ producing potential of E. coli, we employed previous known increasing strategies for systematic metabolic engineering. These include the supplementation with substrate precursors and the co-expression of rate-limiting genes. The co-expression of dxs-ubiA and the supplementation with substrate precursors such as pyruvate (PYR) and parahydroxybenzoic acid (pHBA) increased the content of CoQ(8) in E. coli (ΔmenA) by 125 and 59 %, respectively. Moreover, a 180 % increase in the CoQ(8) content in E. coli (ΔmenA) was realized by the combination of the co-expression of dxs-ubiA and the supplementation with PYR and pHBA. All in all, CoQ(8) content in E. coli increased 4.06 times by blocking the menaquinone pathway, dxs-ubiA co-expression and the addition of sodium pyruvate and parahydroxybenzoic acid to the medium. Results suggested a synergistic effect among different metabolic engineering strategies.
authors.affiliation: Institute of Mitochondrial Biology and Medicine, Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi'an Jiao tong University, Xi'an, 710049, Shaanxi, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
authors.forename: Shuiyun
authors.initials: S
authors.lastname: Yang
authors.forename: Junchao
authors.initials: J
authors.lastname: Zhao
authors.forename: Tingting
authors.initials: T
authors.lastname: Su
authors.forename: Liangrui
authors.initials: L
authors.lastname: Zhao
authors.forename: Jiankang
authors.initials: J
authors.lastname: Liu
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D007218:Industrial Microbiology / Q000379:methods*; D060847:Metabolic Engineering / Q000379:methods*; D010226:Parabens; D010957:Plasmids / Q000235:genetics; D014451:Ubiquinone / Q000096:biosynthesis*; D024482:Vitamin K 2 / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D010226:Parabens; D024482:Vitamin K 2; D014451:Ubiquinone; C030778:ubiquinone 8; D019883:Alkyl and Aryl Transferases; C112258:MenA protein, E coli; C038193:4-hydroxybenzoic acid