title: LRRC26 is a functional BK channel auxiliary γ subunit in arterial smooth muscle cells.
abstract: BACKGROUND
  Smooth muscle cell (myocyte) large-conductance calcium (Ca)(2+)-activated potassium (BK) channels are functionally significant modulators of arterial contractility. Arterial myocytes express both pore-forming BKα and auxiliary β1 subunits, which increase channel Ca(2+) sensitivity. Recently, several leucine-rich repeat containing (LRRC) proteins have been identified as auxiliary γ subunits that elevate the voltage sensitivity of recombinant and prostate adenocarcinoma BK channels. LRRC expression and physiological functions in native cell types are unclear.
  
  
  OBJECTIVE
  Investigate the expression and physiological functions of leucine-rich repeat containing protein 26 (LRRC26) in arterial myocytes.
  
  
  RESULTS
  Reverse transcription polymerase chain reaction and Western blotting detected LRRC26 mRNA and protein in cerebral artery myocytes. Biotinylation, immunofluorescence resonance energy transfer microscopy, and coimmunoprecipitation indicated that LRRC26 was located in close spatial proximity to, and associated with, plasma membrane BKα subunits. LRRC26 knockdown (RNAi) reduced total and surface LRRC26, but did not alter BKα or β1, proteins in arteries. LRRC26 knockdown did not alter Ca(2+) sparks but reduced BK channel voltage sensitivity, which decreased channel apparent Ca(2+) sensitivity and transient BK current frequency and amplitude in myocytes. LRRC26 knockdown also increased myogenic tone over a range (40-100 mm Hg) of intravascular pressures, and reduced vasoconstriction to iberiotoxin and vasodilation to NS1619, BK channel inhibitors and activators, respectively. In contrast, LRRC26 knockdown did not alter depolarization (60 mmol/L K(+))-induced vasoconstriction.
  
  
  CONCLUSIONS
  LRRC26 is expressed, associates with BKα subunits, and elevates channel voltage- and apparent Ca(2+) sensitivity in arterial myocytes to induce vasodilation. This study indicates that arterial myocytes express a functional BK channel γ subunit.
authors.affiliation: From the Department of Physiology, University of Tennessee Health Science Center, Memphis.
authors.forename: Kirk W
authors.initials: KW
authors.lastname: Evanson
authors.affiliation: From the Department of Physiology, University of Tennessee Health Science Center, Memphis.
authors.forename: John P
authors.initials: JP
authors.lastname: Bannister
authors.affiliation: From the Department of Physiology, University of Tennessee Health Science Center, Memphis.
authors.forename: M Dennis
authors.initials: MD
authors.lastname: Leo
authors.affiliation: From the Department of Physiology, University of Tennessee Health Science Center, Memphis. jjaggar@uthsc.edu.
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: Jaggar
mesh_terms: D000818:Animals; D015153:Blotting, Western; D020013:Calcium Signaling; D002536:Cerebral Arteries / Q000378:metabolism; D005786:Gene Expression Regulation; D047468:Immunoprecipitation; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046509:Tissue Culture Techniques; D014162:Transfection; D014661:Vasoconstriction; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014664:Vasodilation; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: C539913:LRRC26 protein, human; D051036:Large-Conductance Calcium-Activated Potassium Channels; D009363:Neoplasm Proteins; D021122:Protein Subunits; D012333:RNA, Messenger; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents
keywords: auxiliary subunit BK LRRC26; muscle; potassium channels; smooth, vascular; vasodilation