title: Development of high-performance liquid chromatographic for quality and authenticity control of Chinese propolis.
abstract: A RP-high-performance liquid chromatography (HPLC) method was developed for quality control of Chinese propolis by simultaneous analysis of 12 flavonoids and 8 phenolic acids. The results showed that vanillic acid, rutin, myricetin, and luteolin were not detected in all of the analyzed propolis and poplar tree gum samples. The caffeic acid, ferulic acid and p-coumaric acid were not detected in poplar tree gum but were detected in propolis, which suggest that they are practical indexes of distinguishing propolis from poplar tree gum. The flavonoid profiles of poplar tree gum were found to be similar to those of propolis, which are dominated by pinobanksin, pinocembrin, 3-O-acetylpinobanksin, chrysin, and galangin. Therefore, the proposed method could be applied to exclude poplar tree gum from propolis with cafferic acid, ferulic acid, and p-coumaric acid as qualitative markers, and distinguish poplar source resin from other illegal substances, and evaluate the quality grading of poplar-type propolis with pinobanksin, pinocembrin, 3-O-acetylpinobanksin, chrysin, and galangin as qualitative and quantitative markers.
authors.affiliation: College of Animal Sciences, Zhejiang Univ, Nr. 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Cui-ping
authors.forename: Huang
authors.initials: H
authors.lastname: Shuai
authors.forename: Wei
authors.initials: W
authors.lastname: Wen-ting
authors.forename: Ping
authors.initials: P
authors.lastname: Shun
authors.forename: Shen
authors.initials: S
authors.lastname: Xiao-ge
authors.forename: Li
authors.initials: L
authors.lastname: Ya-jing
authors.forename: Hu
authors.initials: H
authors.lastname: Fu-liang
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D053149:Plant Gums / Q000737:chemistry; D032107:Populus / Q000737:chemistry; D011429:Propolis / Q000737:chemistry
chemical_list: D053149:Plant Gums; D011429:Propolis
keywords: HPLC; flavonoids; phenolic acid; poplar tree gum; quality control