title: In vitro degradability, bioactivity and cell responses to mesoporous magnesium silicate for the induction of bone regeneration.
abstract: Mesoporous magnesium silicate (m-MS) was synthesized, and the in vitro degradability, bioactivity and primary cell responses to m-MS were investigated. The results suggested that the m-MS with mesoporous channels of approximately 5nm possessed the high specific surface area of 451.0m(2)/g and a large specific pore volume of 0.41cm(3)/g compared with magnesium silicate (MS) without mesopores of 75m(2)/g and 0.21cm(3)/g, respectively. The m-MS was able to absorb a large number of water, with water absorption of 74% compared with 26% for MS. The m-MS was also degradable in a Tris-HCl solution, with a weight loss ratio of 40wt% after a 70-day immersion period. The m-MS exhibited good in vitro bioactivity, inducing apatite formation on its surfaces after soaking in simulated body fluid (SBF) at a faster rate than observed for MS. The m-MS surface clearly promoted the proliferation and differentiation of MC3T3-E1 cells, and their normal cell morphology indicated excellent cytocompatibility. This study suggested that mesoporous magnesium silicate with a high specific surface area and pore volume had suitable degradability and good bioactivity and biocompatibility, making it an excellent candidate biomaterial for the induction of bone regeneration.
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Zhaoying
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Tang
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China.
authors.forename: Han
authors.initials: H
authors.lastname: Guo
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Songchao
authors.initials: S
authors.lastname: Tang
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Jue
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Jiacan
authors.initials: J
authors.lastname: Su
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: biomaterbone@163.com.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: jiewei7860@sina.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
mesh_terms: D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001031:Apatites / Q000737:chemistry; D001672:Biocompatible Materials / Q000494:pharmacology*; D001826:Body Fluids / Q000737:chemistry; D001861:Bone Regeneration / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007477:Ions; D017633:Magnesium Silicates / Q000494:pharmacology*; D051379:Mice; D009584:Nitrogen; D010316:Particle Size; D016062:Porosity; D012996:Solutions; D013696:Temperature; D014867:Water; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D007477:Ions; D017633:Magnesium Silicates; D012996:Solutions; D014867:Water; D000469:Alkaline Phosphatase; D009584:Nitrogen
keywords: Bioactive materials; Cytocompatibility; Degradation; Mesoporous magnesium silicate; Water absorption