title: Notch1 is an important mediator for enhancing of B-cell activation and antibody secretion by Notch ligand.
abstract: The roles of Notch1 and Notch2 in T-cell function have been well studied, but the functional roles of Notch in B cells have not been extensively investigated, except for Notch2 involvement in peripheral marginal zone B-cell differentiation. This study examined the roles of Notch1 in murine primary B cells. During B-cell activation by B-cell receptor ligation, Notch1 was up-regulated while Notch2 was not. In addition, Notch1 up-regulation itself did not contribute to the further activation of B cells, but the Notch ligand was important for Notch1-mediated further B-cell activation. Moreover, Notch1 deficiency significantly decreased B-cell activation and antibody secretion under the presence of Notch ligand. These data suggest that Notch1 is an important mediator for enhancing B-cell activation and antibody secretion by Notch ligand.
authors.affiliation: School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea; Bio Imaging Research Centre and Immune Synapse Research Centre, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea.
authors.forename: Jung-Ah
authors.initials: JA
authors.lastname: Kang
authors.forename: Woo-Seok
authors.initials: WS
authors.lastname: Kim
authors.forename: Sung-Gyoo
authors.initials: SG
authors.lastname: Park
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000235:genetics* / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D017353:Gene Deletion; D015870:Gene Expression; D055786:Gene Knockout Techniques; D006801:Humans; D008024:Ligands; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology*; D051379:Mice; D010641:Phenotype; D054730:Protein Interaction Domains and Motifs; D051881:Receptor, Notch1 / Q000819:agonists / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism
chemical_list: D008024:Ligands; D051881:Receptor, Notch1; D011947:Receptors, Antigen, B-Cell
keywords: B cell; B-cell receptor; Notch ligand; Notch1