title: Genetic variants in human leukocyte antigen-DP influence both hepatitis C virus persistence and hepatitis C virus F protein generation in the Chinese Han population.
abstract: Chronic hepatitis C is a serious liver disease that often results in cirrhosis or hepatocellular carcinoma. The aim of this study was to assess the association of human leukocyte antigen-DP (HLA-DP) variants with risk of chronic hepatitis C virus (HCV) or anti-F antibody generation. We selected two single nucleotide polymorphisms (SNPs) in a region including HLA-DPA1 (rs3077) and HLA-DPB1 (rs9277534) and genotyped SNPs in 702 cases and 342 healthy controls from the Chinese population using TaqMan SNP genotyping assay. Moreover, the exon 2 of the HLA-DPA1 and HLA-DPB1 genes were amplified and determined by sequencing-based typing (SBT). The results showed that rs3077 significantly increased the risk of chronic HCV infection in additive models and dominant models (odds ratio (OR) = 1.32 and 1.53). The rs3077 also contributed to decrease the risk of anti-F antibody generation in additive models and dominant models (OR = 0.46 and 0.56). Subsequent analyses revealed the risk haplotypes (DPA1*0103-DPB1*0501 and DPA1*0103-DPB1*0201) and protective haplotypes (DPA1*0202-DPB1*0501 and DPA1*0202-DPB1*0202) to chronic HCV infection. Moreover, we also found that the haplotype of DPA1*0103-DPB1*0201 and DPA1*0202-DPB1*0202 were associated with the anti-F antibody generation. Our findings show that genetic variants in HLA-DP gene are associated with chronic HCV infection and anti-F antibody generation.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China. dongxiaoxuok@163.com.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xu
authors.affiliation: School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China. njym08@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Yue
authors.affiliation: Department of Infectious Diseases, the First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China. phoenix@126.com.
authors.forename: Longfeng
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China. dengxiaozhao1002@vip.sina.com.
authors.forename: Xiaozhao
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Infectious Diseases, the First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China. zyx3019@163.com.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Disease Control and Prevention, Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China. liuxiaolinok914@126.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China. zhudanyan1907@126.com.
authors.forename: Danyan
authors.initials: D
authors.lastname: Zhu
authors.affiliation: School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China. xiaowen2013cpu@163.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Department of Clinical Laboratory, Nanjing Second Hospital, Nanjing 210003, China. zzx0417@126.com.
authors.forename: Zhenxian
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Pharmacology, Nantong University Medical College, Nantong 226019, China. juanwenyy@ntu.edu.cn.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Yao
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China. cpukj@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Kong
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China. yuxiaojie0903@126.com.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China. juanwei_315@163.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Wei
mesh_terms: D000368:Aged; D000914:Antibodies, Viral / Q000276:immunology; D002681:China / Q000453:epidemiology; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D059867:HLA-DP alpha-Chains / Q000235:genetics* / Q000276:immunology; D059868:HLA-DP beta-Chains / Q000235:genetics* / Q000276:immunology; D006239:Haplotypes; D016174:Hepacivirus / Q000276:immunology*; D019698:Hepatitis C, Chronic / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D059867:HLA-DP alpha-Chains; D059868:HLA-DP beta-Chains; C071172:HLA-DPA1 antigen; C071170:HLA-DPB1 antigen; D014764:Viral Proteins