title: Effect of CTGF/CCN2 on osteo/cementoblastic and fibroblastic differentiation of a human periodontal ligament stem/progenitor cell line.
abstract: Appropriate mechanical loading during occlusion and mastication play an important role in maintaining the homeostasis of periodontal ligament (PDL) tissue. Connective tissue growth factor (CTGF/CCN2), a matricellular protein, is known to upregulate extracellular matrix production, including collagen in PDL tissue. However, the underlying mechanisms of CTGF/CCN2 in regulation of PDL tissue integrity remain unclear. In this study, we investigated the effect of CTGF/CCN2 on osteo/cementoblastic and fibroblastic differentiation of human PDL stem cells using the cell line 1-11. CTGF/CCN2 expression in rat PDL tissue and human PDL cells (HPDLCs) was confirmed immunohisto/cytochemically. Mechanical loading was found to increase gene expression and secretion of CTGF/CCN2 in HPDLCs. CTGF/CCN2 upregulated the proliferation and migration of 1-11 cells. Furthermore, increased bone/cementum-related gene expression in this cell line led to mineralization. In addition, combined treatment of 1-11 cells with CTGF/CCN2 and transforming growth factor-β1 (TGF-β1) significantly promoted type I collagen and fibronectin expression compared with that of TGF-β1 treatment alone. Thus, these data suggest the underlying biphasic effects of CTGF/CCN2 in 1-11 cells, inducible osteo/cementoblastic, and fibroblastic differentiation dependent on the environmental condition. CTGF/CCN2 may contribute to preservation of the structural integrity of PDL tissue, implying its potential use as a therapeutic agent for PDL regeneration.
authors.affiliation: Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Yuda
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Maeda
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Fujii
authors.forename: Satoshi
authors.initials: S
authors.lastname: Monnouchi
authors.forename: Naohide
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Naohisa
authors.initials: N
authors.lastname: Wada
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Koori
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tomokiyo
authors.forename: Sayuri
authors.initials: S
authors.lastname: Hamano
authors.forename: Daigaku
authors.initials: D
authors.lastname: Hasegawa
authors.forename: Akifumi
authors.initials: A
authors.lastname: Akamine
mesh_terms: D000328:Adult; D000818:Animals; D002113:Calcification, Physiologic / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor / Q000096:biosynthesis / Q000494:pharmacology*; D003739:Dental Cementum / Q000166:cytology; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000166:cytology*; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology*; D013312:Stress, Physiological; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D055815:Young Adult
chemical_list: C526317:CCN2 protein, human; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor