title: Immunogenicity of peanut proteins containing poly(anhydride) nanoparticles.
abstract: In the last decade, peanut allergy has increased substantially. Significant differences in the prevalence among different countries are attributed to the type of thermal processing. In spite of the high prevalence and the severe reaction induced by peanuts, there is no immunotherapy available. The aim of this work was to evaluate the potential application of poly(anhydride) nanoparticles (NPs) as immunoadjuvants for peanut oral immunotherapy. NPs loaded with raw or roasted peanut proteins were prepared by a solvent displacement method and dried by either lyophilization or spray-drying. After physicochemical characterization, their adjuvant capacity was evaluated after oral immunization of C57BL/6 mice. All nanoparticle formulations induced a balanced T(H)1 and T(H)2 antibody response, accompanied by low specific IgE induction. In addition, oral immunization with spray-dried NPs loaded with peanut proteins was associated with a significant decrease in splenic T(H)2 cytokines (interleukin 4 [IL-4], IL-5, and IL-6) and enhancement of both T(H)1 (gamma interferon [IFN-γ]) and regulatory (IL-10) cytokines. In conclusion, oral immunization with poly(anhydride) NPs, particularly spray-dried formulations, led to a pro-T(H)1 immune response.
authors.affiliation: Adjuvant Unit, Department of Pharmacy and Pharmaceutical Technology, and Department of Microbiology, University of Navarra, Pamplona, Spain.
authors.forename: Juliana
authors.initials: J
authors.lastname: De S Rebouças
authors.affiliation: Adjuvant Unit, Department of Pharmacy and Pharmaceutical Technology, and Department of Microbiology, University of Navarra, Pamplona, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Irache
authors.affiliation: Adjuvant Unit, Department of Pharmacy and Pharmaceutical Technology, and Department of Microbiology, University of Navarra, Pamplona, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Camacho
authors.affiliation: Department of Allergy and Clinical Immunology, Clinica Universidad de Navarra, Pamplona, Spain.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Gastaminza
authors.affiliation: Department of Allergy and Clinical Immunology, Clinica Universidad de Navarra, Pamplona, Spain.
authors.forename: María L
authors.initials: ML
authors.lastname: Sanz
authors.affiliation: Department of Allergy and Clinical Immunology, Clinica Universidad de Navarra, Pamplona, Spain mferrerp@unav.es cgamazo@unav.es.
authors.forename: Marta
authors.initials: M
authors.lastname: Ferrer
authors.affiliation: Adjuvant Unit, Department of Pharmacy and Pharmaceutical Technology, and Department of Microbiology, University of Navarra, Pamplona, Spain mferrerp@unav.es cgamazo@unav.es.
authors.forename: Carlos
authors.initials: C
authors.lastname: Gamazo
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D010367:Arachis / Q000276:immunology*; D005260:Female; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007167:Immunotherapy / Q000379:methods*; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D021183:Peanut Hypersensitivity / Q000276:immunology / Q000628:therapy*; D010940:Plant Proteins / Q000276:immunology*; D049388:Polyanhydrides / Q000737:chemistry*; D011897:Random Allocation; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; C556062:IL10 protein, mouse; D015848:Interleukin-5; D015850:Interleukin-6; D010940:Plant Proteins; D049388:Polyanhydrides; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma