title: A glucan isolated from flowers of Lonicera japonica Thunb. inhibits aggregation and neurotoxicity of Aβ42.
abstract: Inhibition of Aβ aggregation and attenuation of its cytotoxicity are considered to valuable therapeutics for Alzheimer's disease (AD). Here, a glucan named as LJW0F2 was purified from flowers of Lonicera japonica Thunb. Using monosaccharides composition analysis, methylation analysis, IR and NMR spectroscopy, this polysaccharide was elucidated to be an α-D-(1→4)-glucan with an α-(1→4) linked branch attached to the C-6 position. Its inhibitory effect on Aβ42 aggregation was measured by fluorescence spectroscopic analysis with thioflavine T (ThT) and atomic force microscopy (AFM). We showed that polysaccharide LJW0F2 could inhibit Aβ42 aggregation in a dose-dependent-manner. Besides, LJW0F2 could attenuate the cytotoxicity induced by Aβ42 aggregation in SH-SY5Y neuroblastoma cells. To the best of our knowledge, this was the first report that the exogenous plant-derived polysaccharide might block Aβ42 aggregation directly and reduce its toxicity in SH-SY5Y cells.
authors.affiliation: Glycochemistry & Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Glycochemistry & Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Liao
authors.affiliation: Glycochemistry & Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Fang
authors.affiliation: Glycochemistry & Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Glycochemistry & Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yao
authors.affiliation: Infinitus Co., Ltd, Guangzhou 510623, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Hu
authors.affiliation: Glycochemistry & Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Electronic address: dingkan@simm.ac.cn.
authors.forename: Kan
authors.initials: K
authors.lastname: Ding
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification* / Q000494:pharmacology; D035264:Flowers; D005936:Glucans / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D031239:Lonicera; D010446:Peptide Fragments / Q000633:toxicity*
chemical_list: D016229:Amyloid beta-Peptides; D004365:Drugs, Chinese Herbal; D005936:Glucans; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)
keywords: Aggregation and neurotoxicity; Alzheimer's disease; Aβ(42); Glucan; Lonicera japonica Thunb.