title: High-grade mutant OmpF induces decreased bacterial survival rate.
abstract: OmpF plays very important roles in the influx of antibiotics and bacterial survival in the presence of antibiotics. However, high-grade mutant OmpF and its function in decreasing bacterial survival rate have not been reported to date. In the present study, we cloned a high-grade mutant OmpF (mOmpF) and sequence analysis suggested that over 45 percent of the DNA sequence was significantly mutated, leading to dramatic changes in over 55 percent of the amino acid sequence. mOmpF protein was successfully expressed. When grown in the presence of antibiotic, the bacterial survival rate decreased and the antibiotic inhibition zone became larger with the increase of the mOmpF. It was concluded that concentration of high-grade mutant mOmpF dramatically influenced the bacterial survival rate. The study presented here may provide insights into better understanding of the relationships between structure and function of OmpF.
authors.affiliation: School of Chemistry and Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, China.
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Zhao
authors.affiliation: School of Chemistry and Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, China.
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Liu
authors.affiliation: School of Chemistry and Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Journals Department, Chongqing University, Chongqing, China.
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.affiliation: School of Chemistry and Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Nie
authors.affiliation: School of Chemistry and Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, China.
authors.forename: Zai-xin
authors.initials: ZX
authors.lastname: Li
authors.affiliation: College of Horticulture and Landscape Agriculture, Southwest University, Beibei, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Pan
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007612:Kanamycin / Q000494:pharmacology; D050296:Microbial Viability / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D018272:Porins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D000900:Anti-Bacterial Agents; C109237:OmpF protein; D018272:Porins; D011994:Recombinant Proteins; D007612:Kanamycin