title: Transcriptome profiling of pyrethroid resistant and susceptible mosquitoes in the malaria vector, Anopheles sinensis.
abstract: BACKGROUND
  Anopheles sinensis is a major malaria vector in China and other Southeast Asian countries, and it is becoming increasingly resistant to the insecticides used for agriculture, net impregnation, and indoor residual spray. Very limited genomic information on this species is available, which has hindered the development of new tools for resistance surveillance and vector control. We used the 454 GS FLX system and generated expressed sequence tag (EST) databases of various life stages of An. sinensis, and we determined the transcriptional differences between deltamethrin resistant and susceptible mosquitoes.
  
  
  RESULTS
  The 454 GS FLX transcriptome sequencing yielded a total of 624,559 reads (average length of 290 bp) with the pooled An. sinensis mosquitoes across various development stages. The de novo assembly generated 33,411 contigs with average length of 493 bp. A total of 8,057 ESTs were generated with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation. A total of 2,131 ESTs were differentially expressed between deltamethrin resistant and susceptible mosquitoes collected from the same field site in Jiangsu, China. Among these differentially expressed ESTs, a total of 294 pathways were mapped to the KEGG database, with the predominant ESTs belonging to metabolic pathways. Furthermore, a total of 2,408 microsatellites and 15,496 single nucleotide polymorphisms (SNPs) were identified.
  
  
  CONCLUSIONS
  The annotated EST and transcriptome databases provide a valuable genomic resource for further genetic studies of this important malaria vector species. The differentially expressed ESTs associated with insecticide resistance identified in this study lay an important foundation for further functional analysis. The identified microsatellite and SNP markers will provide useful tools for future population genetic and comparative genomic analyses of malaria vectors.
authors.forename: Guoding
authors.initials: G
authors.lastname: Zhu
authors.forename: Daibin
authors.initials: D
authors.lastname: Zhong
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Huayun
authors.initials: H
authors.lastname: Zhou
authors.forename: Julin
authors.initials: J
authors.lastname: Li
authors.forename: Yaobao
authors.initials: Y
authors.lastname: Liu
authors.forename: Liang
authors.initials: L
authors.lastname: Bai
authors.forename: Sui
authors.initials: S
authors.lastname: Xu
authors.forename: Mei-Hui
authors.initials: MH
authors.lastname: Wang
authors.forename: Guofa
authors.initials: G
authors.lastname: Zhou
authors.forename: Xuelian
authors.initials: X
authors.lastname: Chang
authors.affiliation: Department of Parasitology, Medical College of Soochow University, Suzhou 215123, PR China. gaoqi54@hotmail.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
authors.forename: Guiyun
authors.initials: G
authors.lastname: Yan
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000254:growth & development*; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D049750:Genome, Insect; D007305:Insecticide Resistance; D007306:Insecticides / Q000009:adverse effects*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011722:Pyrethrins / Q000009:adverse effects*
chemical_list: D007306:Insecticides; D011722:Pyrethrins