title: Role of autophagy and mTOR signaling in neural differentiation of bone marrow mesenchymal stem cells.
abstract: Autophagy is involved in cell differentiation. We present evidence that autophagy is activated during β-mercaptoethanol (β-ME)-induced neuronal differentiation of bone marrow mesenchymal stem cells (MSCs), in which mammalian target of rapamycin (mTOR) signaling is important. mTOR activity declined after being transported from the nucleus to the cytoplasm. Using 3-methyladenine (3-MA) and rapamycin to regulate the activity of mTOR, it was found that the efficiency of neuronal differentiation was affected.
authors.affiliation: Department of Neurology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Li
authors.forename: Cuiqin
authors.initials: C
authors.lastname: Wang
authors.forename: Guangyu
authors.initials: G
authors.lastname: Zhang
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Wang
authors.forename: Ranran
authors.initials: R
authors.lastname: Duan
authors.forename: Huili
authors.initials: H
authors.lastname: Gao
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Junfang
authors.initials: J
authors.lastname: Teng
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Jia
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001343:Autophagy / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D015536:Down-Regulation / Q000187:drug effects; D008623:Mercaptoethanol / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D055495:Neurogenesis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: C025946:3-methyladenine; D008623:Mercaptoethanol; D058570:TOR Serine-Threonine Kinases; D000225:Adenine; D020123:Sirolimus
keywords: MSCs; autophagy; mTOR; neuronal differentiation