title: Novel validated spectrofluorimetric methods for the determination of taurine in energy drinks and human urine.
abstract: Two sensitive, selective, economic and validated spectrofluorimetric methods were developed for the determination of taurine in energy drinks and spiked human urine. Method Ι is based on fluorimetric determination of the amino acid through its reaction with Hantzsch reagent to form a highly fluorescent product measured at 490 nm after excitation at 419 nm. Method ΙΙ is based on the reaction of taurine with tetracyanoethylene yielding a fluorescent charge transfer complex, which was measured at λex /em of (360 nm/450 nm). The proposed methods were subjected to detailed validation procedures, and were statistically compared with the reference method, where the results obtained were in good agreement. Method Ι was further applied to determine taurine in energy drinks and spiked human urine giving promising results. Moreover, the stoichiometry of the reactions was studied, and reaction mechanisms were postulated.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
authors.forename: M K
authors.initials: MK
authors.lastname: Sharaf El Din
authors.forename: M E K
authors.initials: ME
authors.lastname: Wahba
mesh_terms: D061215:Energy Drinks / Q000032:analysis*; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013654:Taurine / Q000032:analysis* / Q000652:urine*
chemical_list: D013654:Taurine
keywords: Hantzsch reagent; energy drinks; taurine; tetracyanoethylene