title: Comparative analysis of lycorine in wild plant and callus culture samples of Hymenocallis littoralis by HPLC-UV method.
abstract: The Hymenocallis littoralis, an ornamental and medicinal plant, had been traditionally used for wound healing. In the present study, an analytical method using HPLC with ultraviolet detection was developed for the quantification of lycorine in the extracts of different parts of wild plant and tissue culture samples of H. littoralis. The separation was achieved using a reversed-phase column. The method was found to be accurate, repeatable, and sensitive for the quantification of minute amount of lycorine present in the samples. The highest lycorine content was found in the bulb extract (2.54 ± 0.02 μg/mg) whereas the least was in the root extract (0.71 ± 0.02 μg/mg) of the wild plants. Few callus culture samples had high content of lycorine, comparable to that of wild plants. The results showed that plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D) alone at 4.5 μM (2.58 ± 0.38 μg/mg) or a combination of 2,4-D at 9.00 μM with 4.5 μM of 6-benzylaminopurine (BAP), were the optimum concentrations for the production of high lycorine (2.45 ± 0.15 μg/mg) content in callus culture. The present analytical method could be of value for routine quantification of lycorine in the tissue culture production and standardization of the raw material or extracts of H. littoralis.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors.forename: Sreeramanan
authors.identifier: 0000-0003-4392-285X
authors.initials: S
authors.lastname: Subramaniam
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia ; AIMST University, Jalan Bedong, Semeling, 08100 Bedong, Kedah Darul Aman, Malaysia.
authors.forename: Jeevandran
authors.identifier: 0000-0002-4546-8112
authors.initials: J
authors.lastname: Sundarasekar
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors.forename: Geethaa
authors.initials: G
authors.lastname: Sahgal
authors.affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors.forename: Vikneswaran
authors.initials: V
authors.lastname: Murugaiyah
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D047151:Amaryllidaceae Alkaloids / Q000032:analysis*; D001593:Benzyl Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007701:Kinetin / Q000494:pharmacology; D010617:Phenanthridines / Q000032:analysis*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry* / Q000187:drug effects; D011687:Purines
chemical_list: D047151:Amaryllidaceae Alkaloids; D001593:Benzyl Compounds; D010617:Phenanthridines; D010937:Plant Growth Regulators; D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; C015330:lycorine; C480551:benzylaminopurine; D007701:Kinetin