title: Analysis of chemosensory gene families in the beetle Monochamus alternatus and its parasitoid Dastarcus helophoroides.
abstract: We assembled antennal transcriptomes of pest Monochamus alternatus and its parasitoid Dastarcus helophoroides to identify the members of the major chemosensory multi-gene families. Gene ontology (GO) annotation indicated that the relative abundance of transcripts associated with specific GO terms was highly similar in the two species. In chemosensory gene families, we identified 52 transcripts encoding putative odorant-binding proteins (OBPs), 19 chemosensory proteins (CSPs), 10 olfactory receptors (ORs), 8 ionotropic receptors (IRs), 2 gustatory receptors (GRs), and 5 sensory neuron membrane proteins (SNMPs) in these two transcriptomes. Predicted protein sequences were compared with Dendroctonus ponderosae, Tribolium castaneum and Drosophila melanogaster. The results of phylogenetic trees showed that some clusters included only OBPs or CSPs from D. helophoroides, some clusters included only OBPs or CSPs from M. alternatus, while some clusters included OBPs or CSPs from both M. alternatus and D. helophoroides. The identification of the chemosensory genes and the phylogenetic relationship of these genes between two species might provide new ideas for controlling M. alternatus and improving current strategies for biological control.
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Dong-Zhen
authors.initials: DZ
authors.lastname: Li
authors.affiliation: Hubei Ecology Vocational College, Wuhan 430200, People's Republic of China.
authors.forename: Shui-Fa
authors.initials: SF
authors.lastname: Min
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Mi
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Zhou
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: mqwang@mail.hzau.edu.cn.
authors.forename: Man-Qun
authors.initials: MQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000737:chemistry; D001517:Coleoptera / Q000737:chemistry / Q000235:genetics*; D005260:Female; D019476:Insect Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D008297:Male; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D018035:Receptors, Odorant / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D059467:Transcriptome
chemical_list: D019476:Insect Proteins; D018035:Receptors, Odorant
keywords: Dastarcus helophoroides; Monochamus alternatus; Neighbor-joining trees; Olfactory proteins; Transcriptome