title: Oral administration of Lactobacillus paracasei alleviates clinical symptoms of colitis induced by dextran sulphate sodium salt in BALB/c mice.
abstract: The aim of this study was to investigate the alleviating effect of Lactobacillus paracasei subsp. paracasei LC-01 (LC-01) on the murine model of colitis induced by dextran sulphate sodium (DSS). 50 pathogen-free, 6-week-old male BALB/c mice were divided randomly into 5 groups, including a control group and four DSS-LC-01-treated groups (DSS, DSS-106, DSS-108, and DSS-1010 with 0, 1×106, 1×108 and 1×1010 cfu/ml LC-01, respectively). To test the effectiveness of LC-01 as a prophylactic it was administered for 7 days before the onset of the disease in DSS-LC-01-treated mice. After 7 days, colitis was induced by administration of 2.5% (w/v) DSS in drinking water for a further 7 days. The disease activity index (DAI), histological score, myeloperoxidase (MPO) activity and the level of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumour necrosis factor α (TNF-α) were measured. DAI, histological scores and MPO activity of mice treated with a medium or high dose of LC-01 were significantly lower compared to a low-dose of LC-01 and DSS treatment alone (P<0.05). Colon length shortening could be prevented with increasing dose of LC-01. In addition, the levels of IL-1β and TNF-α were suppressed significantly by treatment with a medium and high dose of LC-01. However, no significant difference in the indices mentioned above were observed between a low dose of LC-01 and treatment with DSS alone (P≯0.05). An appropriate dose of LC-01 can prevent intestinal damage in mice with DSS-induced colitis. The expression of inflammatory cytokines related to pathogenesis of DSS-induced colitis decreased following treatment with LC-01.
authors.affiliation: Beijing Laboratory for Food Quality and Safety, and Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, P.O. Box 303, No. 17 Tsinghua East Road, Beijing 100083, China P.R.
authors.forename: T
authors.initials: T
authors.lastname: Pan
authors.affiliation: Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Tsinghua East Road, Beijing 100083, China P.R.
authors.forename: H Y
authors.initials: HY
authors.lastname: Guo
authors.affiliation: Beijing Laboratory for Food Quality and Safety, and Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, P.O. Box 303, No. 17 Tsinghua East Road, Beijing 100083, China P.R.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Mengniu Dairy (Beijing) Company, Beijing 011500, China P.R.
authors.forename: A P
authors.initials: AP
authors.lastname: Liu
authors.affiliation: College of Food Science and Technology Engineering, Gansu Agricultural University, Lanzhou 730070, China P.R.
authors.forename: X X
authors.initials: XX
authors.lastname: Wang
authors.affiliation: Beijing Laboratory for Food Quality and Safety, and Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, P.O. Box 303, No. 17 Tsinghua East Road, Beijing 100083, China P.R. Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Tsinghua East Road, Beijing 100083, China P.R.
authors.forename: F Z
authors.initials: FZ
authors.lastname: Ren
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D003106:Colon / Q000473:pathology*; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D005243:Feces; D007249:Inflammation / Q000188:drug therapy* / Q000517:prevention & control; D053583:Interleukin-1beta / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000473:pathology; D007778:Lactobacillus / Q000145:classification*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009195:Peroxidase / Q000096:biosynthesis; D019936:Probiotics / Q000008:administration & dosage / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D016264:Dextran Sulfate; D009195:Peroxidase
keywords: DSS-induced colitis; Lactobacillus paracasei; animal model; pro-inflammatory cytokine