title: Electrocatalytic miRNA detection using cobalt porphyrin-modified reduced graphene oxide.
abstract: Metalated porphyrins have been described to bind nucleic acids. Additionally, cobalt porphyrins present catalytic properties towards oxygen reduction. In this work, a carboxylic acid-functionalized cobalt porphyrin was physisorbed on reduced graphene oxide, then immobilized on glassy carbon electrodes. The carboxylic groups were used to covalently graft amino-terminated oligonucleotide probes which are complementary to a short microRNA target. It was shown that the catalytic oxygen electroreduction on cobalt porphyrin increases upon hybridization of miRNA strand ("signal-on" response). Current changes are amplified compared to non-catalytic amperometric system. Apart from oxygen, no added reagent is necessary. A limit of detection in the sub-nanomolar range was reached. This approach has never been described in the literature.
authors.affiliation: University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. ca.desouza@yahoo.fr.
authors.forename: Camille
authors.initials: C
authors.lastname: De Souza
authors.affiliation: University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. samia.zrig@univ-paris-diderot.fr.
authors.forename: Samia
authors.initials: S
authors.lastname: Zrig
authors.affiliation: University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. dengjun.wang@univ-paris-diderot.fr.
authors.forename: Dengjun
authors.initials: D
authors.lastname: Wang
authors.affiliation: University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. mcpham@univ-paris-diderot.fr.
authors.forename: Minh-Chau
authors.initials: MC
authors.lastname: Pham
authors.affiliation: University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. piro@univ-paris-diderot.fr.
authors.forename: Benoit
authors.initials: B
authors.lastname: Piro
mesh_terms: D003035:Cobalt / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry; D015336:Molecular Probe Techniques; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry*
chemical_list: D035683:MicroRNAs; D010087:Oxides; D011166:Porphyrins; D003035:Cobalt; D006108:Graphite