title: A lipidomic perspective on intermediates in cholesterol synthesis as indicators of disease status.
abstract: Lipidomics is increasingly becoming a viable method for researchers to routinely identify the various sterols present in samples, beyond just measuring cholesterol itself. In particular, the measurement of intermediates in cholesterol synthesis can shed new insights into not only the flux through the pathway, but also numerous disease states where levels of sterol intermediates are drastically altered. In this review, we indicate several intermediates that are relevant to disease, and discuss the challenges for analysing them, including the need for standardised methodology or universal controls across the lipidomics field.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Sharpe
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Vicky
authors.initials: V
authors.lastname: Burns
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia. Electronic address: aj.brown@unsw.edu.au.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Brown
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D006505:Hepatitis / Q000378:metabolism; D006801:Humans; D008659:Metabolic Diseases / Q000378:metabolism; D055432:Metabolomics; D013261:Sterols / Q000096:biosynthesis*
chemical_list: D015415:Biomarkers; D013261:Sterols
keywords: Cholesterol; Intermediates; Lipidomics