title: Crystallization and preliminary X-ray crystallographic studies of the ArsI C-As lyase from Thermomonospora curvata.
abstract: Arsenic is a ubiquitous and carcinogenic environmental element that enters the biosphere primarily from geochemical sources, but also through anthropogenic activities. Microorganisms play an important role in the arsenic biogeochemical cycle by biotransformation of inorganic arsenic into organic arsenicals and vice versa. ArsI is a microbial nonheme ferrous-dependent dioxygenase that transforms toxic methylarsonous acid to the less toxic inorganic arsenite by C-As bond cleavage. An ArsI ortholog from the thermophilic bacterium Thermomonospora curvata was expressed, purified and crystallized. The crystals diffracted to 1.46 Å resolution and belonged to space group P4₃2₁2 or its enantiomer P4₁2₁2, with unit-cell parameters a=b=42.2, c=118.5 Å.
authors.affiliation: Department of Cellular Biology and Pharmacology, Florida International University, Herbert Wertheim College of Medicine, Miami, FL 33199, USA.
authors.forename: S Venkadesh
authors.initials: SV
authors.lastname: Nadar
authors.affiliation: Department of Cellular Biology and Pharmacology, Florida International University, Herbert Wertheim College of Medicine, Miami, FL 33199, USA.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yoshinaga
authors.affiliation: SER-CAT and the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
authors.forename: Palani
authors.initials: P
authors.lastname: Kandavelu
authors.affiliation: Berkeley Center for Structural Biology, Lawrence Berkeley Laboratory, Berkeley, CA 94720, USA.
authors.forename: Banumathi
authors.initials: B
authors.lastname: Sankaran
authors.affiliation: Department of Cellular Biology and Pharmacology, Florida International University, Herbert Wertheim College of Medicine, Miami, FL 33199, USA.
authors.forename: Barry P
authors.initials: BP
authors.lastname: Rosen
mesh_terms: D000192:Actinomycetales / Q000201:enzymology*; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D008190:Lyases / Q000737:chemistry*
chemical_list: D017931:DNA Primers; D008190:Lyases
keywords: ArsI; Thermomonospora curvata; arsenic