title: Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients.
abstract: Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, and is characterized by abnormalities in ovarian angiogenesis, among other features. Consistent with this association, follicular fluid (FF) concentration and ovarian expression of vascular endothelial growth factor (VEGF) are increased in PCOS patients. In this study, we examined the protein levels of platelet-derived growth factor (PDGF) BB and DD (PDGFBB and PDGFDD), angiopoietin 1 and 2 (ANGPT1 and ANGPT2), and their soluble receptor sTIE2 in FF from PCOS and control patients undergoing assisted reproductive techniques. We also analyzed the effect of FF from PCOS and control patients on tight and adherens junction protein expression in an endothelial cell line. PDGFBB and PDGFDD were significantly lower whereas ANGPT1 concentration was significantly higher in FF from PCOS patients than from control patients. No changes were found in the concentration of ANGPT2 or sTIE2. Expression of claudin-5 was significantly increased in endothelial cells incubated for 24 hr in the presence of FF from PCOS versus from control patients, while vascular-endothelial cadherin, β-catenin, and zonula occludens 1 expression were unchanged. The changes observed in the levels of PDGF isoforms and ANGPT1 may prevent VEGF-induced vascular permeability in the PCOS ovary by regulating endothelial-cell-junction protein levels. Restoring the levels of angiogenic factors may provide new insights into PCOS treatment and the prevention of ovarian hyperstimulation syndrome in affected women.
authors.affiliation: Instituto de Biología y Medicina Experimental-CONICET, Buenos Aires, Argentina.
authors.forename: Leopoldina
authors.initials: L
authors.lastname: Scotti
authors.forename: Fernanda
authors.initials: F
authors.lastname: Parborell
authors.forename: Griselda
authors.initials: G
authors.lastname: Irusta
authors.forename: Ignacio
authors.initials: I
authors.lastname: De Zuñiga
authors.forename: Claudio
authors.initials: C
authors.lastname: Bisioli
authors.forename: Hernan
authors.initials: H
authors.lastname: Pettorossi
authors.forename: Marta
authors.initials: M
authors.lastname: Tesone
authors.forename: Dalhia
authors.initials: D
authors.lastname: Abramovich
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism; D000328:Adult; D042683:Angiopoietin-1 / Q000378:metabolism*; D042702:Angiopoietin-2 / Q000378:metabolism*; D000077214:Becaplermin; D015153:Blotting, Western; D062507:Claudin-5 / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D016471:Ovarian Hyperstimulation Syndrome / Q000517:prevention & control; D010053:Ovary / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism*; D011863:Radioimmunoassay; D027724:Reproductive Techniques, Assisted; D019108:Tight Junctions / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C467630:ANGPT1 protein, human; C586513:ANGPT2 protein, human; D042683:Angiopoietin-1; D042702:Angiopoietin-2; C107466:CLDN5 protein, human; D062507:Claudin-5; D020574:Proto-Oncogene Proteins c-sis; D042461:Vascular Endothelial Growth Factor A; D000077214:Becaplermin; D011374:Progesterone; D004958:Estradiol