title: From laboratory to field: OsNRAMP5-knockdown rice is a promising candidate for Cd phytoremediation in paddy fields.
abstract: Previously, we reported that OsNRAMP5 functions as a manganese, iron, and cadmium (Cd) transporter. The shoot Cd content in OsNRAMP5 RNAi plants was higher than that in wild-type (WT) plants, whereas the total Cd content (roots plus shoots) was lower. For efficient Cd phytoremediation, we produced OsNRAMP5 RNAi plants using the natural high Cd-accumulating cultivar Anjana Dhan (A5i). Using a positron-emitting tracer imaging system, we assessed the time-course of Cd absorption and accumulation in A5i plants. Enhanced 107Cd translocation from the roots to the shoots was observed in A5i plants. To evaluate the phytoremediation capability of A5i plants, we performed a field experiment in a Cd-contaminated paddy field. The biomass of the A5i plants was unchanged by the suppression of OsNRAMP5 expression; the A5i plants accumulated twice as much Cd in their shoots as WT plants. Thus, A5i plants could be used for rapid Cd extraction and the efficient phytoremediation of Cd from paddy fields, leading to safer food production.
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Takahashi
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan; Graduate School of Science, Tohoku University, Aoba-ku, Sendai, Miyagi, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ishimaru
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Hugo
authors.initials: H
authors.lastname: Shimo
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Khurram
authors.initials: K
authors.lastname: Bashir
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Senoura
authors.affiliation: Agrogenomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Sugimoto
authors.affiliation: Agrogenomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Ono
authors.affiliation: Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Suzui
authors.affiliation: Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Kawachi
authors.affiliation: Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Ishii
authors.affiliation: Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma, Japan.
authors.forename: Yong-Gen
authors.initials: YG
authors.lastname: Yin
authors.affiliation: Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma, Japan.
authors.forename: Shu
authors.initials: S
authors.lastname: Fujimaki
authors.affiliation: Agrogenomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yano
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan; Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Nonoichi-shi, Ishikawa, Japan.
authors.forename: Naoko K
authors.initials: NK
authors.lastname: Nishizawa
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nakanishi
mesh_terms: D001673:Biodegradation, Environmental; D001692:Biological Transport; D002104:Cadmium / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D019216:Metals, Heavy / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D034622:RNA Interference
chemical_list: D027682:Cation Transport Proteins; D019216:Metals, Heavy; D002104:Cadmium