title: Highly sensitive and selective photoelectrochemical biosensor platform for polybrominated diphenyl ether detection using the quantum dots sensitized three-dimensional, macroporous ZnO nanosheet photoelectrode.
abstract: A novel photoelectrochemical (PEC) immunosensor for the rapid detection of 2,3',4,5',6-pentabromodiphenylether (BDE-121) was developed by coating a core-shell ZnS/CdTe/Mn-CdS/ZnS sensitized macroporous ZnO nanosheet (NS) photoelectrode with anti-BDE-121 polyclonal antibody. Here, core-shell CdTe/Mn-CdS quantum dots (QDs) benefit the signal amplification and photostability of the co-sensitized ZnO NS photoelectrode. After introducing the ZnS buffer layers between different interfaces (the ZnO NS and sensitizers; sensitizers and the electrolyte), the photoresponse was further enhanced. Under standard simulated illumination, the saturation photocurrent density of the co-sensitized photoelectrode is 6.23 mA cm(-2), the highest value reported to date for ZnO NS based photoelectrode. The BDE-121 was detected by monitoring the changes of the photocurrent signals of the immunosensor resulting from the immunoreaction. The immunosensor is sensitive, stable and highly specific toward BDE-121, displaying a linear range of 5 pM to 100 nM with a limit of detection of 3.98 pM. BDE-121 in paint samples was analyzed with the proposed sensor and gas chromatography-mass spectrometry (GC/MS), giving contents of 1.32 ± 0.02 ng mL(-1) and 1.16 ng mL(-1), respectively.
authors.affiliation: State Key Lab of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China.
authors.forename: Weili
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Lab of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China.
authors.forename: Pengtao
authors.initials: P
authors.lastname: Sheng
authors.affiliation: State Key Lab of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Cai
authors.affiliation: State Key Lab of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Feng
authors.affiliation: State Key Lab of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China. Electronic address: qycai0001@hnu.cn.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Cai
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D005411:Flame Retardants / Q000032:analysis*; D055768:Halogenated Diphenyl Ethers / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation; D008027:Light; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D045663:Quantum Dots / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; C000591838:2,3',4,5',6-pentabromodiphenyl ether; D015034:Zinc Oxide
keywords: Immunosensor; Macroporous ZnO nanosheet; Photoelectrochemical; Polybrominated diphenyl ether; Quantum dots