title: Short-term UV-B dose stimulates production of protective metabolites in Matricaria chamomilla leaves.
abstract: Physiological response of two cultivars of Matricaria chamomilla plants on UV irradiation was studied. The impact of used short-time UV dose was evaluated in three time points; 2, 24 and 48 h after irradiation. Used UV irradiation immediately resulted in changes in plant oxidative status monitored as increased concentration of H2 O2 . Decrease in chlorophyll a and b indicated the impact on photosynthetic apparatus. For phenolic secondary metabolites, an increase in total soluble phenols and AlCl3 -reactive flavonols was observed. The activity of main phenolic enzyme, phenylalanine ammonia-lyase, increased with time after irradiation. Significant changes, mainly decreasing trends, in the content of free coumarins and their glycosidic precursors were observed. Enhanced accumulation in chlorogenic and 1,5-dicaffeoylquinic acid and in (Z)-isoform of dicycloethers was detected. From these results, the redirecting precursors of coumarin biosynthesis to biosynthesis of substances with higher antioxidative potential can be assumed. Different reactions in diploid and tetraploid plants were recorded, too.
authors.affiliation: Department of Botany, Pavol Jozef Šafárik University, Košice, Slovak Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Petruľová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Dučaiová
authors.forename: Miroslav
authors.initials: M
authors.lastname: Repčák
mesh_terms: D002726:Chlorogenic Acid / Q000819:agonists / Q000378:metabolism; D002734:Chlorophyll / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D000077194:Chlorophyll A; D002934:Cinnamates / Q000819:agonists / Q000378:metabolism; D003374:Coumarins / Q000037:antagonists & inhibitors / Q000378:metabolism; D044948:Flavonols / Q000819:agonists* / Q000096:biosynthesis; D006861:Hydrogen Peroxide / Q000378:metabolism; D031168:Matricaria / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D018384:Oxidative Stress; D010636:Phenols / Q000819:agonists* / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism*; D010788:Photosynthesis / Q000502:physiology / Q000528:radiation effects*; D010940:Plant Proteins / Q000819:agonists* / Q000378:metabolism; D011003:Ploidies; D011837:Radiation-Protective Agents / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D002934:Cinnamates; D003374:Coumarins; D044948:Flavonols; D010636:Phenols; D010940:Plant Proteins; D011837:Radiation-Protective Agents; D002734:Chlorophyll; D002726:Chlorogenic Acid; C037184:chlorophyll b; C100257:cynarine; D006861:Hydrogen Peroxide; D010650:Phenylalanine Ammonia-Lyase; D000077194:Chlorophyll A