title: Usefulness of the TRC method in the peritoneal washing cytology for gastric cancer.
abstract: BACKGROUND
  Peritoneal dissemination from gastric cancer is the most frequent metastasis of advanced gastric cancer. Detection of cancer micrometastasis is required for improvement of cancer therapy. A method that can detect micrometastases more sensitively is desired. Against this background, for the purposes of making the genetic diagnosis of micrometastasis simple and rapid, TRC (transcription reverse transcription concerted reaction) was developed.
  
  
  METHODS
  69 patients with gastric cancer in those diagnosed with deeper than mp. At the time of surgery, peritoneal washing with saline was extracted. Having extracted the RNA therein, It was blended with a reagent responsive to CEAmRNA and was put into TRC. The cytology and TRC were compared and studied.
  
  
  RESULTS
  The concordance rate between TRC and cytology was K=0.6552. The patients whom there was a discrepancy between the cytology and TRC are clinically validate the TRC results. In the study of prognosis, TRC obtained a sensitivity of 90.9% and a specificity of 98.3%.
  
  
  CONCLUSIONS
  The measurement of CEAmRNA in peritoneal washing specimens by the TRC method is superior to Cytology. That is also equal to or superior to the RT-PCR method with respect to sensitivity and specificity, and superior with respect to simplicity and rapidity.
authors.forename: Masako
authors.initials: M
authors.lastname: Nishizawa
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Seshimo
authors.forename: Kunitomo
authors.initials: K
authors.lastname: Miyake
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Amano
authors.forename: Shingo
authors.initials: S
authors.lastname: Kameoka
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003581:Cytodiagnosis / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D061206:Neoplasm Micrometastasis; D010533:Peritoneal Lavage; D010534:Peritoneal Neoplasms / Q000175:diagnosis*; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000473:pathology*