title: Efficient proteolysis and application of an alkaline protease from halophilic Bacillus sp. EMB9.
abstract: A salt-stable alkaline protease from moderately halophilic Bacillus sp. EMB9, isolated from the western coast of India, is described. This protease was capable of efficiently removing silver from used/waste X-Ray films, as well as hydrolyzing defatted soy flour with 31% degree of hydrolysis (DH). Production of the protease was optimized by using response surface methodology. Ca(2+) and NaCl were the most critical factors in enhancing the yield. Under optimized culture conditions, a maximum of 369 U protease/mL was obtained, which is quite comparable to the yields of commercial proteases. The elevated production level coupled with ability to efficiently hydrolyze protein-laden soy flour and complete recovery of silver from used X-Ray films makes it a prospective industrial enzyme.
authors.affiliation: a Enzyme and Microbial Biochemistry Laboratory, Department of Chemistry , Indian Institute of Technology , Delhi , New Delhi , India.
authors.forename: Rajeshwari
authors.initials: R
authors.lastname: Sinha
authors.forename: A K
authors.initials: AK
authors.lastname: Srivastava
authors.forename: S K
authors.initials: SK
authors.lastname: Khare
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D010450:Endopeptidases / Q000378:metabolism*; D005433:Flour; D006868:Hydrolysis; D007194:India; D015233:Models, Statistical; D011492:Protein Hydrolysates; D012834:Silver / Q000302:isolation & purification* / Q000378:metabolism; D045730:Soy Foods; D014962:X-Ray Film
chemical_list: D001426:Bacterial Proteins; D011492:Protein Hydrolysates; D012834:Silver; D010450:Endopeptidases; C087822:alkaline protease
keywords: Bacillus sp.; RSM; halophilic; hydrolysates; protease; soy flour