title: [Advanced glycation end products inhibit testosterone production in rat Leydig cells].
abstract: OBJECTIVE
  To study the expression of the receptor for advanced glycation end products (RAGE) and the inhibitory effect of advanced glycation end products (AGEs) on testosterone production in rat Leydig cells.
  
  
  METHODS
  Rat Leydig cells were primarily cultured and the expression of RAGE in the Leydig cells was detected by RT-PCR and immunofluorescence staining. The Leydig cells were treated with AGEs at the concentrations of 25, 50, 100 and 200 microg/ml, respectively, and the testosterone content was determined by ELISA.
  
  
  RESULTS
  RT-PCR and immunofluorescence staining exhibited the expression of RAGE in the rat Leydig cells. AGEs remarkably suppressed hCG-induced testosterone production in the Leydig cells in a concentration-dependent manner in the 50, 100 and 200 microg/ml groups as compared with the control (P < 0.01).
  
  
  CONCLUSIONS
  RAGE exists in rat Leydig cells and AGEs can significantly inhibit the secretion of testosterone in primarily cultured rat Leydig cells.
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Qi
authors.forename: Chuan-Yin
authors.initials: CY
authors.lastname: Hu
authors.forename: Shao-Hong
authors.initials: SH
authors.lastname: Chen
authors.forename: You
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D007985:Leydig Cells / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D051381:Rats; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013739:Testosterone / Q000096:biosynthesis*
chemical_list: D017127:Glycation End Products, Advanced; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D013739:Testosterone