title: Determination of synthetic dyes in bean and meat products by liquid chromatography with tandem mass spectrometry.
abstract: A sensitive and efficient method was developed for the simultaneous determination of eight synthetic dyes (Chrysoidin, Auramine O, Sudan(I-IV), Para Red, and Rhodamine B) in bean and meat products using high-performance liquid chromatography with tandem mass spectrometry. A simple extraction procedure using acetonitrile has been applied for the extraction of these dyes from spiked bean and meat samples. Chromatographic separation was achieved on a Waters XTerra C18 column (2.1 × 150 mm, 5 μm) with a multistep gradient elution. Detection and quantification were performed using mass spectrometry in multiple reaction monitoring mode. Linear calibrations were obtained with correlation coefficients R(2) > 0.99. The limits of detection and quantification for the eight dyes were in the ranges of 0.03-0.75 and 0.1-2.0 μg/kg depending on matrices, respectively. The recoveries of these dyes in different food matrices were between 71.2 and 116.9% with relative standard deviations <15.2%, suggesting that the developed method is promising for the accurate quantification of the eight dyes at trace levels in bean and meat products.
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, China; New Drug Research & Development Center, Zhengzhou University, Zhengzhou, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ding
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Zheng
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.forename: Hongmin
authors.initials: H
authors.lastname: Liu
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis* / Q000138:chemical synthesis; D007887:Fabaceae / Q000737:chemistry*; D005399:Fishes; D005506:Food Contamination / Q000032:analysis; D008461:Meat Products / Q000032:analysis; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004396:Coloring Agents
keywords: Bean products; Liquid chromatography with mass spectrometry; Meat products; Multiresidue analysis; Synthetic dyes