title: Enzyme-antibody dual labeled gold nanoparticles probe for ultrasensitive detection of κ-casein in bovine milk samples.
abstract: A dual labeled probe was synthesized by coating gold nanoparticles (AuNPs) with anti-κ-CN monoclonal antibody (McAb) and horseradish peroxidase (HRP) enzyme on their surface. The McAb was used as detector and HRP was used as label for signal amplification catalytically oxidize the substrate. AuNPs were used as bridges between the McAb and HRP. Based on the probe, an immunoassay was developed for ultrasensitive detection of κ-CN in bovine milk samples. The assay has a linear response range within 4.2-560 ng mL(-1). The limit of detection (LOD) was 4.2 ng mL(-1) which was 10 times lower than that of traditional McAb-HRP based ELISA. The recoveries of κ-CN from three brand bovine milk samples were from 95.8% to 111.0% that had a good correlation (R(2)=0.998) with those obtained by official standard Kjeldahl method. For higher sensitivity and as simple as the traditional ELISA, the developed immunoassay could provide an alternative approach for ultrasensitive detection of κ-CN in bovine milk sample.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China. Electronic address: zhouyu69@sina.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Meng
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Production Quality Test Institute of Jilin Province, Changchun 130022, PR China.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Liu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: N N
authors.initials: NN
authors.lastname: Wang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: P
authors.initials: P
authors.lastname: Hu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Lu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: H L
authors.initials: HL
authors.lastname: Ren
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Z S
authors.initials: ZS
authors.lastname: Liu
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002364:Caseins / Q000032:analysis*; D002417:Cattle; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D002364:Caseins; D004800:Enzymes, Immobilized; D006046:Gold; D006735:Horseradish Peroxidase
keywords: Antibody; Gold nanoparticles; Horseradish peroxidase; Probe; κ-CN