title: Zerumbone suppresses IL-1β-induced cell migration and invasion by inhibiting IL-8 and MMP-3 expression in human triple-negative breast cancer cells.
abstract: Inflammation is a key regulatory process in cancer development. Prolonged exposure of breast tumor cells to inflammatory cytokines leads to epithelial-mesenchymal transition, which is the principal mechanism involved in metastasis and tumor invasion. Interleukin (IL)-1β is a major inflammatory cytokine in a variety of tumors. To date, the regulatory mechanism of IL-1β-induced cell migration and invasion has not been fully elucidated. Here, we investigated the effect of zerumbone (ZER) on IL-1β-induced cell migration and invasion in breast cancer cells. The levels of IL-8 and matrix metalloproteinase (MMP)-3 mRNA were analyzed by real-time polymerase chain reaction. The levels of secreted IL-8 and MMP-3 protein were analyzed by enzyme-linked immunosorbent assay and western blot analysis, respectively. Cell invasion and migration was detected by Boyden chamber assay. The levels of IL-8 and MMP-3 expression were significantly increased by IL-1β treatment in Hs578T and MDA-MB231 cells. On the other hand, IL-1β-induced IL-8 and MMP-3 expression was decreased by ZER. Finally, IL-1β-induced cell migration and invasion were decreased by ZER in Hs578T and MDA-MB231 cells. ZER suppresses IL-1β-induced cell migration and invasion by inhibiting IL-8 expression and MMP-3 expression in TNBC cells. ZER could be a promising therapeutic drug for treatment of triple-negative breast cancer patients.
authors.affiliation: Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-dong, Gangnam-gu, Seoul, Korea; Department of Life Science, Hanyang University, Seoul, 133-791, Korea.
authors.forename: Jeonghun
authors.initials: J
authors.lastname: Han
authors.forename: Soo Youn
authors.initials: SY
authors.lastname: Bae
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Oh
authors.forename: Jeongmin
authors.initials: J
authors.lastname: Lee
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Hyun-Chul
authors.initials: HC
authors.lastname: Lee
authors.forename: Se Kyung
authors.initials: SK
authors.lastname: Lee
authors.forename: Won Ho
authors.initials: WH
authors.lastname: Kil
authors.forename: Seok Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Seok Jin
authors.initials: SJ
authors.lastname: Nam
authors.forename: Sangmin
authors.initials: S
authors.lastname: Kim
authors.forename: Jeong Eon
authors.initials: JE
authors.lastname: Lee
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D016209:Interleukin-8 / Q000378:metabolism*; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D012717:Sesquiterpenes / Q000494:pharmacology*; D064726:Triple Negative Breast Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: C508606:CXCL8 protein, human; D053583:Interleukin-1beta; D016209:Interleukin-8; D012717:Sesquiterpenes; C403304:zerumbone; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3
keywords: IL-1β; IL-8; MMP-3; triple-negative breast cancer; zerumbone