title: Chronic impact of sulfamethoxazole on acetate utilization kinetics and population dynamics of fast growing microbial culture.
abstract: The study evaluated the chronic impact of sulfamethoxazole on metabolic activities of fast growing microbial culture. It focused on changes induced on utilization kinetics of acetate and composition of the microbial community. The experiments involved a fill and draw reactor, fed with acetate and continuous sulfamethoxazole dosing of 50 mg/L. The evaluation relied on model evaluation of the oxygen uptake rate profiles, with parallel assessment of microbial community structure by 454-pyrosequencing. Continuous sulfamethoxazole dosing inflicted a retardation effect on acetate utilization in a way commonly interpreted as competitive inhibition, blocked substrate storage and accelerated endogenous respiration. A fraction of acetate was utilized at a much lower rate with partial biodegradation of sulfamethoxazole. Results of pyrosequencing with a replacement mechanism within a richer more diversified microbial culture, through inactivation of vulnerable fractions in favor of species resistant to antibiotic, which made them capable of surviving and competing even with a slower metabolic response.
authors.affiliation: Istanbul Technical University, Civil Engineering Faculty, Department of Environmental Engineering, 34469 Maslak, Istanbul, Turkey. Electronic address: gkor@itu.edu.tr.
authors.forename: G
authors.initials: G
authors.lastname: Kor-Bicakci
authors.affiliation: Istanbul Technical University, Civil Engineering Faculty, Department of Environmental Engineering, 34469 Maslak, Istanbul, Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Pala-Ozkok
authors.affiliation: Department of Environmental Health Sciences, University Medical Center Freiburg, Breisacher Strasse 115B, D-79106 Freiburg i.Br, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Rehman
authors.affiliation: Department of Environmental Health Sciences, University Medical Center Freiburg, Breisacher Strasse 115B, D-79106 Freiburg i.Br, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Jonas
authors.affiliation: Istanbul Technical University, Civil Engineering Faculty, Department of Environmental Engineering, 34469 Maslak, Istanbul, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Ubay-Cokgor
authors.affiliation: Istanbul Technical University, Civil Engineering Faculty, Department of Environmental Engineering, 34469 Maslak, Istanbul, Turkey; ENVIS Energy and Environmental Systems Ltd., ITU Arı Teknokent, Arı 1 Binası, 16, 34469 Maslak, Istanbul, Turkey.
authors.forename: D
authors.initials: D
authors.lastname: Orhon
mesh_terms: D000085:Acetates / Q000378:metabolism*; D020561:Alphaproteobacteria / Q000187:drug effects / Q000254:growth & development; D001483:Base Sequence; D019149:Bioreactors; D049109:Cell Proliferation / Q000187:drug effects; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003198:Computer Simulation; D007700:Kinetics; D064307:Microbiota / Q000187:drug effects* / Q000235:genetics; D008954:Models, Biological; D008969:Molecular Sequence Data; D010101:Oxygen Consumption / Q000187:drug effects; D010230:Paracoccus / Q000187:drug effects / Q000254:growth & development; D011157:Population Dynamics; D017422:Sequence Analysis, DNA; D013420:Sulfamethoxazole / Q000633:toxicity*
chemical_list: D000085:Acetates; D013420:Sulfamethoxazole
keywords: 454-Pyrosequencing; Biodegradation; Chronic inhibition; Process kinetics; Sulfamethoxazole