title: Chromatin protein HP1 interacts with the mitotic regulator borealin protein and specifies the centromere localization of the chromosomal passenger complex.
abstract: Accurate mitosis requires the chromosomal passenger protein complex (CPC) containing Aurora B kinase, borealin, INCENP, and survivin, which orchestrates chromosome dynamics. However, the chromatin factors that specify the CPC to the centromere remain elusive. Here we show that borealin interacts directly with heterochromatin protein 1 (HP1) and that this interaction is mediated by an evolutionarily conserved PXVXL motif in the C-terminal borealin with the chromo shadow domain of HP1. This borealin-HP1 interaction recruits the CPC to the centromere and governs an activation of Aurora B kinase judged by phosphorylation of Ser-7 in CENP-A, a substrate of Aurora B. Consistently, modulation of the motif PXVXL leads to defects in CPC centromere targeting and aberrant Aurora B activity. On the other hand, the localization of the CPC in the midzone is independent of the borealin-HP1 interaction, demonstrating the spatial requirement of HP1 in CPC localization to the centromere. These findings reveal a previously unrecognized but direct link between HP1 and CPC localization in the centromere and illustrate the critical role of borealin-HP1 interaction in orchestrating an accurate cell division.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Song
authors.forename: Yuda
authors.initials: Y
authors.lastname: Huo
authors.forename: Jiahai
authors.initials: J
authors.lastname: Zhang
authors.forename: Tongge
authors.initials: T
authors.lastname: Zhu
authors.forename: Jianyu
authors.initials: J
authors.lastname: Wang
authors.forename: Xuannv
authors.initials: X
authors.lastname: Zhao
authors.forename: Felix
authors.initials: F
authors.lastname: Aikhionbare
authors.forename: Jiancun
authors.initials: J
authors.lastname: Zhang
authors.forename: Hequan
authors.initials: H
authors.lastname: Duan
authors.forename: Jihui
authors.initials: J
authors.lastname: Wu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Dou
authors.forename: Yunyu
authors.initials: Y
authors.lastname: Shi
authors.forename: Xuebiao
authors.initials: X
authors.lastname: Yao
mesh_terms: D020816:Amino Acid Motifs; D064107:Aurora Kinase B / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology*; D002503:Centromere / Q000235:genetics / Q000378:metabolism*; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D002877:Chromosomes, Human / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D017434:Protein Structure, Tertiary
chemical_list: C490157:CDCA8 protein, human; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000090266:Chromobox Protein Homolog 5; C576921:AURKB protein, human; D064107:Aurora Kinase B