title: Cytokine detection by flow cytometry.
abstract: Analysis of intracellular cytokines is extremely important in the clinical treatment of numerous diseases. Flow cytometry (FCM) is a highly effective technique that detects intracellular cytokines using specific fluorescence-labeled antibodies. The common steps of this assay include cell collection, fixation, permeabilization, blocking, intracellular staining and analysis by FCM. This technique also allows for analyzing the biological function of cytokines. In this chapter, we describe a modified method to detect the specific intracellular cytokine staining using FCM, with an emphasis on the effects of variables including samples, temperature, buffers, data acquisition, and analysis.
authors.affiliation: Department of Cell Biology and Institute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
authors.forename: Jian-Ge
authors.initials: JG
authors.lastname: Qiu
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Mei
authors.forename: Zhe-Sheng
authors.initials: ZS
authors.lastname: Chen
authors.forename: Zhi
authors.initials: Z
authors.lastname: Shi
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D000906:Antibodies / Q000737:chemistry; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D008985:Monensin / Q000494:pharmacology; D061209:Proton Ionophores / Q000494:pharmacology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000906:Antibodies; D016209:Interleukin-8; D061209:Proton Ionophores; D008985:Monensin