title: A high-density, high-channel count, multiplexed μECoG array for auditory-cortex recordings.
abstract: Our understanding of the large-scale population dynamics of neural activity is limited, in part, by our inability to record simultaneously from large regions of the cortex. Here, we validated the use of a large-scale active microelectrode array that simultaneously records 196 multiplexed micro-electrocortigraphical (μECoG) signals from the cortical surface at a very high density (1,600 electrodes/cm(2)). We compared μECoG measurements in auditory cortex using a custom "active" electrode array to those recorded using a conventional "passive" μECoG array. Both of these array responses were also compared with data recorded via intrinsic optical imaging, which is a standard methodology for recording sound-evoked cortical activity. Custom active μECoG arrays generated more veridical representations of the tonotopic organization of the auditory cortex than current commercially available passive μECoG arrays. Furthermore, the cortical representation could be measured efficiently with the active arrays, requiring as little as 13.5 s of neural data acquisition. Next, we generated spectrotemporal receptive fields from the recorded neural activity on the active μECoG array and identified functional organizational principles comparable to those observed using intrinsic metabolic imaging and single-neuron recordings. This new electrode array technology has the potential for large-scale, temporally precise monitoring and mapping of the cortex, without the use of invasive penetrating electrodes.
authors.affiliation: Department of Psychology, University of Connecticut, Storrs, Connecticut; Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut; Department of Electrical Engineering, University of Connecticut, Storrs, Connecticut;
authors.forename: Monty A
authors.initials: MA
authors.lastname: Escabí
authors.affiliation: Department of Psychology, University of Connecticut, Storrs, Connecticut; Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut;
authors.forename: Heather L
authors.initials: HL
authors.lastname: Read
authors.affiliation: Center for Neural Science, New York University, New York, New York; Department of Electrical and Computer Engineering, Polytechnic Institute of New York University, Brooklyn, New York;
authors.forename: Jonathan
authors.initials: J
authors.lastname: Viventi
authors.affiliation: Center for Nanoparticle Research of Institute for Basic Science, School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea;
authors.forename: Dae-Hyeong
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Psychology, University of Connecticut, Storrs, Connecticut;
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Higgins
authors.affiliation: Department of Psychology, University of Connecticut, Storrs, Connecticut;
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Storace
authors.affiliation: Bioengineering Graduate Group, University of Pennsylvania, Philadelphia, Pennsylvania;
authors.forename: Andrew S K
authors.initials: AS
authors.lastname: Liu
authors.affiliation: Neuroscience Graduate Group, University of Pennsylvania, Philadelphia, Pennsylvania;
authors.forename: Adam M
authors.initials: AM
authors.lastname: Gifford
authors.affiliation: Neuroscience Graduate Group, University of Pennsylvania, Philadelphia, Pennsylvania;
authors.forename: John F
authors.initials: JF
authors.lastname: Burke
authors.affiliation: Department of Electrical and Computer Engineering, Polytechnic Institute of New York University, Brooklyn, New York;
authors.forename: Matthew
authors.initials: M
authors.lastname: Campisi
authors.affiliation: Department of Materials Science and Engineering, Beckman Institute for Advanced Science and Technology and Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois;
authors.forename: Yun-Soung
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Electrical and Systems Engineering, University of Pennsylvania, Philadelphia, Pennsylvania;
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Avrin
authors.affiliation: Department of Electrical and Systems Engineering, University of Pennsylvania, Philadelphia, Pennsylvania;
authors.forename: Van der
authors.initials: Vd
authors.lastname: Spiegel Jan
authors.affiliation: Departments of Mechanical Engineering and Civil and Environmental Engineering, Northwestern University, Evanston, Illinois;
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Structural Analysis for Industrial Equipment, Dalian University of Technology, Dalian, China;
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Engineering Mechanics, Tsinghua University, Beijing, China;
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Materials Science and Engineering, Beckman Institute for Advanced Science and Technology and Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois;
authors.forename: John A
authors.initials: JA
authors.lastname: Rogers
authors.affiliation: Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania; Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania;
authors.forename: Brian
authors.initials: B
authors.lastname: Litt
authors.affiliation: Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania; and Department of Otorhinolaryngology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania ycohen@mail.med.upenn.edu.
authors.forename: Yale E
authors.initials: YE
authors.lastname: Cohen
mesh_terms: D000818:Animals; D001303:Auditory Cortex / Q000502:physiology*; D001931:Brain Mapping / Q000295:instrumentation* / Q000379:methods; D004569:Electroencephalography / Q000295:instrumentation* / Q000379:methods; D005072:Evoked Potentials, Auditory; D008297:Male; D008839:Microelectrodes; D061848:Optical Imaging / Q000379:methods; D051381:Rats
keywords: auditory cortex; electrocorticography; tonotopy; topography; μECoG