title: Secondary ion mass spectrometry imaging of Dictyostelium discoideum aggregation streams.
abstract: High resolution imaging mass spectrometry could become a valuable tool for cell and developmental biology, but both, high spatial and mass spectral resolution are needed to enable this. In this report, we employed Bi3 bombardment time-of-flight (Bi3 ToF-SIMS) and C60 bombardment Fourier transform ion cyclotron resonance secondary ion mass spectrometry (C60 FTICR-SIMS) to image Dictyostelium discoideum aggregation streams. Nearly 300 lipid species were identified from the aggregation streams. High resolution mass spectrometry imaging (FTICR-SIMS) enabled the generation of multiple molecular ion maps at the nominal mass level and provided good coverage for fatty acyls, prenol lipids, and sterol lipids. The comparison of Bi3 ToF-SIMS and C60 FTICR-SIMS suggested that while the first provides fast, high spatial resolution molecular ion images, the chemical complexity of biological samples warrants the use of high resolution analyzers for accurate ion identification.
authors.affiliation: Department of Chemistry and Biochemistry, Florida International University, Miami, Florida, United States of America.
authors.forename: John Daniel
authors.initials: JD
authors.lastname: DeBord
authors.affiliation: FOM Institute AMOLF, Science Park 104, Amsterdam, The Netherlands.
authors.forename: Donald F
authors.initials: DF
authors.lastname: Smith
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Anderton
authors.affiliation: FOM Institute AMOLF, Science Park 104, Amsterdam, The Netherlands.
authors.forename: Ron M A
authors.initials: RM
authors.lastname: Heeren
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Paša-Tolić
authors.affiliation: Department of Biology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Richard H
authors.initials: RH
authors.lastname: Gomer
authors.affiliation: Department of Chemistry and Biochemistry, Florida International University, Miami, Florida, United States of America.
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Fernandez-Lima
mesh_terms: D004023:Dictyostelium / Q000235:genetics*; D008055:Lipids / Q000737:chemistry; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*
chemical_list: D008055:Lipids