title: Detection of platelet microRNA expression in patients with diabetes mellitus with or without ischemic stroke.
abstract: OBJECTIVE
  The objective of this study was to investigate the role of plasma and platelet microRNAs in the occurrence of ischemic stroke in patients with diabetes mellitus.
  
  
  METHODS
  miR-223, miR-146a, miR-495, and miR-107 expression in the plasma and platelets, blood glucose concentration, and platelet activation rate were measured in patients with diabetes mellitus and ischemic stroke, diabetes mellitus only, ischemic stroke only, and healthy controls. Platelet activity was measured by flow cytometric measurement of P-selectin expression, while miRNA was measured by real-time PCR.
  
  
  RESULTS
  The expressions of platelet and plasma miR-223 and miR-146a were significantly downregulated in patients with ischemic stroke and diabetes mellitus or diabetes mellitus only, but not in patients with ischemic stroke only compared to healthy controls. The expressions of platelet and plasma miR-495 and miR-107 showed no significant differences among these four groups. The expression of platelet miR-223 and miR-146a significantly correlated with plasma miR-223 and miR-146a levels, blood glucose concentration, and platelet activation rate.
  
  
  CONCLUSIONS
  Hyperglycemia may downregulate the expressions of miR-223 and miR-146a, leading to subsequent platelet activation in patients with diabetes mellitus. Low platelet and plasma miR-223 and miR-146a expression is a risk factor for ischemic stroke in Chinese diabetes mellitus patients.
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Duan
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhan
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Bingxin
authors.initials: B
authors.lastname: Song
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Sian
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Long
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Neurology, Second Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Hunan Key laboratory of Pharmacogenetics, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Qidong
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China. Electronic address: xiajian1216@sina.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Xia
mesh_terms: D000328:Adult; D001773:Blood Cells / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D002545:Brain Ischemia / Q000097:blood / Q000150:complications / Q000235:genetics*; D016022:Case-Control Studies; D048909:Diabetes Complications / Q000097:blood / Q000235:genetics; D003920:Diabetes Mellitus / Q000097:blood / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D020521:Stroke / Q000097:blood / Q000150:complications / Q000235:genetics*
chemical_list: D035683:MicroRNAs
keywords: Diabetes mellitus; Hyperglycemia; Ischemic stroke; MicroRNA; Platelet