title: Expression of orphan receptors GPR22 and GPR162 in streptozotocin-induced diabetic rats.
abstract: OBJECTIVE
  Diabetes mellitus is a chronic degenerative disease characterized by high blood glucose levels as a result of problems in the action or insulin secretion. Although there are many treatments for this pathology, it has been associated with a high mortality rate. For this reason, it is important to try to identify new pathways that could be involved in diabetic complications. Recently, a new class of receptors has been reported, called orphan receptors because the associated ligand and signaling pathways are unknown, these receptors have been associated with certain pathologies. Therefore, the aim of this work was to study the expression of the orphan receptors GPR22 and GPR162 in heart, aorta, brain and kidney of diabetic rats.
  
  
  METHODS
  We used Wistar male rats with 10-12 weeks of age. Diabetes was induced by a single dose of streptozotocin (60 mg/kg i.p.). After four weeks, the tissue was obtained and the expression of the mRNA was measured by RT-PCR.
  
  
  RESULTS
  Our results showed that the orphan receptors are expressed in a different way in heart, kidney, brain and aorta of diabetic and non-diabetic rats.
  
  
  CONCLUSIONS
  We conclude that orphan receptors could be involved in the development of diabetes complications.
authors.affiliation: Laboratorio de Señalización Intracelular, Sección de Posgrado, Escuela Superior de Medicina del Instituto Politécnico Nacional , México .
authors.forename: Armando
authors.initials: A
authors.lastname: Ruiz-Hernández
authors.forename: Fausto
authors.initials: F
authors.lastname: Sánchez-Muñoz
authors.forename: Jessica
authors.initials: J
authors.lastname: Rodriguez
authors.forename: Loranda
authors.initials: L
authors.lastname: Calderón-Zamora
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Romero-Nava
authors.forename: Fengyang
authors.initials: F
authors.lastname: Huang
authors.forename: Enrique
authors.initials: E
authors.lastname: Hong
authors.forename: Santiago
authors.initials: S
authors.lastname: Villafaña
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D001921:Brain / Q000378:metabolism / Q000473:pathology; D048909:Diabetes Complications / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: C531502:GPR22 protein, rat; D043562:Receptors, G-Protein-Coupled
keywords: Diabetes; GPR162; GPR22; orphan receptors