title: Oxidative balance in lymphocytes from patients with nonalcoholic steatohepatitis.
abstract: Oxidative stress is linked to several human diseases, including nonalcoholic steatohepatitis (NASH). In this study, lymphocytes were used as a model to study this disease. These cells offer several advantages for cellular and molecular studies such as easy accessibility, and they are easily accessible and constitute a "time-persistent" system capable of reflecting the condition of the whole organism. Lymphocytes from patients with NASH display oxidative stress features. Among the possible causes for the overproduction of reactive oxygen species in NASH lymphocytes, there might be alterations of enzymatic pathways, auto-oxidation of glucose and mitochondrial superoxide production, which, in turn, would lead to protein oxidative damage. Increased oxidative stress in lymphocytes from patients with NASH may result in a pro-oxidative environment, which, in turn, could modify the pathway of the enzymatic activities. The data confirm that an imbalance between pro-oxidant and antioxidant defense mechanisms may be an important factor in NASH.
authors.affiliation: Departments of Cellular and Environmental Biology (SD, EU, SP, VM) and Clinical and Experimental Medicine, Internal Medicine, Angiology and Atherosclerosis (GL, GV, ARR), University of Perugia, Perugia, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Belia
authors.forename: Graziana
authors.initials: G
authors.lastname: Lupattelli
authors.forename: Eleonora
authors.initials: E
authors.lastname: Urbani
authors.forename: Gaetano
authors.initials: G
authors.lastname: Vaudo
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Roscini
authors.forename: Stefano
authors.initials: S
authors.lastname: Perni
authors.forename: Valeria
authors.initials: V
authors.lastname: Marsili
mesh_terms: D000328:Adult; D005234:Fatty Liver / Q000453:epidemiology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease; D009765:Obesity / Q000175:diagnosis / Q000453:epidemiology / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055815:Young Adult
chemical_list: D017382:Reactive Oxygen Species