title: Neuroprotective effects of corn silk maysin via inhibition of H2O2-induced apoptotic cell death in SK-N-MC cells.
abstract: OBJECTIVE
  Neuroprotective effects of maysin, which is a flavone glycoside that was isolated from the corn silk (CS, Zea mays L.) of a Korean hybrid corn Kwangpyeongok, against oxidative stress (H2O2)-induced apoptotic cell death of human neuroblastoma SK-N-MC cells were investigated.
  
  
  METHODS
  Maysin cytotoxicity was determined by measuring cell viability using MTT and lactate dehydrogenase (LDH) assays. Intracellular reactive oxygen species (ROS) were measured using a 2,7-dichlorofluorescein diacetate (DCF-DA) assay. Apoptotic cell death was monitored by annexin V-FITC/PI double staining and by a TUNEL assay. Antioxidant enzyme mRNA levels were determined by real-time PCR. The cleavage of poly (ADP-ribose) polymerase (PARP) was measured by western blotting.
  
  
  RESULTS
  Maysin pretreatment reduced the cytotoxic effect of H2O2 on SK-N-MC cells, as shown by the increase in cell viability and by reduced LDH release. Maysin pretreatment also dose-dependently reduced the intracellular ROS level and inhibited PARP cleavage. In addition, DNA damage and H2O2-induced apoptotic cell death were significantly attenuated by maysin pretreatment. Moreover, maysin pretreatment (5-50 μg/ml) for 2h significantly and dose-dependently increased the mRNA levels of antioxidant enzymes (CAT, GPx-1, SOD-1, SOD-2 and HO-1) in H2O2 (200 μM)-insulted cells.
  
  
  CONCLUSIONS
  These results suggest that CS maysin has neuroprotective effects against oxidative stress (H2O2)-induced apoptotic death of human brain SK-N-MC cells through its antioxidative action. This report is the first regarding neuroprotective health benefits of corn silk maysin by its anti-apoptotic action and by triggering the expression of intracellular antioxidant enzyme systems in SK-N-MC cells.
authors.affiliation: Department of Biotechnology, The CUK Agromedical Research Center, The Catholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea.
authors.forename: Doo Jin
authors.initials: DJ
authors.lastname: Choi
authors.affiliation: National Institute of Crop Science, RDA, Suwon, Gyeonggi-do 441-857, Republic of Korea.
authors.forename: Sun-Lim
authors.initials: SL
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, The CUK Agromedical Research Center, The Catholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Choi
authors.affiliation: Department of Biotechnology, The CUK Agromedical Research Center, The Catholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea. Electronic address: yongil382@catholic.ac.kr.
authors.forename: Yong Il
authors.initials: YI
authors.lastname: Park
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000166:cytology* / Q000187:drug effects; D045744:Cell Line, Tumor; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D005960:Glucosides; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C114429:maysin; D006861:Hydrogen Peroxide; D011065:Poly(ADP-ribose) Polymerases
keywords: Antioxidant; Corn silk; Hydrogen peroxide; Maysin; Neuroprotection