title: Apoptotic effect of pheophorbide a-mediated photodynamic therapy on DMBA/TPA-induced mouse papillomas.
abstract: Pheophorbide a (Pa) is a chlorine-based photosensitizer, and Pa-mediated photodynamic therapy (PDT) reportedly exhibits antitumor activity against various malignancies. The aim of our study was to investigate the therapeutic effect of Pa-mediated PDT on 7,12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorobol-13-acetate (TPA)-induced mouse papillomas. Thirty mice received a topical application of DMBA/TPA on their backs to induce mouse papillomas. One week after two sessions of Pa-mediated PDT, immunohistochemical stains and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were performed to evaluate the apoptotic effects thereof on the papillomas. Among 63 mouse papillomas treated with Pa-mediated PDT, 17.5% of the lesions were completely removed 1 week after the first treatment, while 31.7% disappeared 1 week after the second treatment. Statistical analyses revealed significant differences in therapeutic outcomes for the Pa-mediated PDT group in comparison to a solvent-PDT group and a Pa group. Additionally, a marked downregulation of proliferating cell nuclear antigen expression, as well as upregulation of cleaved caspase 3 and cleaved poly(ADP-ribose) polymerase expression, was noted in the Pa-PDT group, compared to the solvent-PDT group and Pa group. TUNEL assay revealed higher apoptotic cell counts in the Pa-PDT group, although the difference was not statistically significant. Our data demonstrated that Pa-mediated PDT is effective in treating DMBA/TPA-induced mouse papillomas.
authors.affiliation: Department of Pathology, Yanbian University Hospital, Yanji City, Jilin Province, China.
authors.forename: Xianglan
authors.initials: X
authors.lastname: Zhang
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Choi
authors.forename: Zhenlong
authors.initials: Z
authors.lastname: Zheng
authors.forename: Lianhua
authors.initials: L
authors.lastname: Zhu
authors.forename: Sung Bin
authors.initials: SB
authors.lastname: Cho
authors.forename: Ki-Yoel
authors.initials: KY
authors.lastname: Kim
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
authors.forename: In-Ho
authors.initials: IH
authors.lastname: Cha
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D063646:Carcinogenesis / Q000187:drug effects / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008812:Mice, Hairless; D010212:Papilloma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology / Q000627:therapeutic use; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate
chemical_list: D017319:Photosensitizing Agents; D018809:Proliferating Cell Nuclear Antigen; D002734:Chlorophyll; D015127:9,10-Dimethyl-1,2-benzanthracene; D053148:Caspase 3; C032623:pheophorbide a; D013755:Tetradecanoylphorbol Acetate