title: Effects of ethyl pyruvate on cardiac function recovery and apoptosis reduction after global cold ischemia and reperfusion.
abstract: The present study used anin vitromodel of cold cardioplegia in isolated working rat hearts to evaluate the possible role of ethyl pyruvate (EP) in promoting cardiac function and preventing apoptosis. Two groups of rats were evaluated; the EP (2 mM EP; n=8) and control (n=8) groups. Isolated rat hearts were perfused with Krebs-Henseleit buffer (KHB) for 30 min, arrested with cardioplegic solution and stored for 4 h in B21 solution at 4°C. The hearts were reperfused with KHB for 45 min. EP was added to the cardioplegic and storage solutions and also to KHB for reperfusion. Cardiac parameters of the heart rate, including left ventricular systolic pressure, left ventricular end-diastolic pressure, left ventricular developed pressure and maximal rise rate of the left ventricular pressure, were monitored. In addition, coronary flow, adenosine triphosphate (ATP) levels and malondialdehyde (MDA) content were recorded and apoptotic cell determination was detected. The functional parameters in the EP group were significantly higher compared with those in the control group during the reperfusion period (P<0.05). In addition, ATP levels were higher in the EP group than in the control group and the content of MDA was lower in the EP group than in the control group. A concentration of 2 mM EP significantly reduced the number of apoptotic cells in the EP group compared with that of the control group (P<0.05). Therefore, EP significantly preserved cardiac function, enhanced tissue ATP levels, attenuated myocardial oxidative injury and markedly reduced apoptosis following myocardial ischemia in anin vitromodel of 4 h of cold cardioplegia and reperfusion.
authors.affiliation: Department of Cardiothoracic Surgery, Taihe Hospital Affiliated to Hubei Medical College, Shiyan, Hubei 442000, P.R. China.
authors.forename: Jialong
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Cardiothoracic Surgery, Taihe Hospital Affiliated to Hubei Medical College, Shiyan, Hubei 442000, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, Taihe Hospital Affiliated to Hubei Medical College, Shiyan, Hubei 442000, P.R. China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Cardiothoracic Surgery, Taihe Hospital Affiliated to Hubei Medical College, Shiyan, Hubei 442000, P.R. China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Cardiothoracic Surgery, Taihe Hospital Affiliated to Hubei Medical College, Shiyan, Hubei 442000, P.R. China.
authors.forename: Chengyi
authors.initials: C
authors.lastname: Lin
authors.affiliation: Department of Cardiovascular Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
authors.forename: Kailun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, Taihe Hospital Affiliated to Hubei Medical College, Shiyan, Hubei 442000, P.R. China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Ji
keywords: apoptosis; ethyl pyruvate; heart; rat; transplantation