title: Anti-tumor and macrophage activation induced by alkali-extracted polysaccharide from Pleurotus ostreatus.
abstract: Pleurotus ostreatus is popularly consumed as traditional medicine and health food for enhancing immune function in China. Polysaccharides from mushroom have been demonstrated to possess a wide range of health beneficial properties. This study was carried out to elucidate the immunomodulating effects and molecular mechanism involved in the in vivo and in vitro anti-tumor activities of alkali-extracted polysaccharide (WPOP-N1) from the fruiting bodies of P. ostreatus. The results showed that WPOP-N1 significantly inhibited the tumor growth of Sarcoma 180 tumor-bearing mice, and markedly increased the secretion level of TNF-α in serum. In addition, WPOP-N1 enhanced the phagocytic capability of peritoneal macrophages in vitro. Furthermore, the secretion of TNF-α and NO and the amount of TNF-α and iNOS transcript were increased significantly when the peritoneal macrophages were exposed to WPOP-N1. Meanwhile, Western blot analysis revealed that the stimulation of peritoneal macrophages by WPOP-N1 induced the phosphorylation of p65 and a marked decrease of IκB expression. These results suggest that WPOP-N1 could activate macrophages through NF-κB signaling pathway, and the anti-tumor effects of WPOP-N1 can be achieved by its immunostimulating property.
authors.affiliation: Life Science Research Center, Beihua University, Jilin 132013, China; Department of Pathophysiology, School of Basic Medical Sciences, Beihua University, Jilin 132013, China.
authors.forename: Fanli
authors.initials: F
authors.lastname: Kong
authors.affiliation: Department of Second Neurology, Affiliated Hospital of Medical College, Beihua University, Jilin 132013, China.
authors.forename: Feng-E
authors.initials: FE
authors.lastname: Li
authors.affiliation: College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Zhongmei
authors.initials: Z
authors.lastname: He
authors.affiliation: Laboratory Medical College, Jilin Medical College, Jilin 132013, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Ruoyi
authors.initials: R
authors.lastname: Hao
authors.affiliation: Life Science Research Center, Beihua University, Jilin 132013, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Life Science Research Center, Beihua University, Jilin 132013, China. Electronic address: tonghaibin@gmail.com.
authors.forename: Haibin
authors.initials: H
authors.lastname: Tong
mesh_terms: D000468:Alkalies / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D006801:Humans; D008262:Macrophage Activation / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010586:Phagocytes / Q000187:drug effects; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012995:Solubility; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014867:Water / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
chemical_list: D000468:Alkalies; D000970:Antineoplastic Agents; D016328:NF-kappa B; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D009569:Nitric Oxide
keywords: Immunomodulation; Pleurotus ostreatus; Polysaccharide