title: Determination of selenium via the fluorescence quenching effect of selenium on hemoglobin-catalyzed peroxidative reaction.
abstract: A new method for the determination of selenium based on its fluorescence quenching on the hemoglobin-catalyzed reaction of H2 O2 and l-tyrosine has been established. The effect of pH, foreign ions and the optimization of variables on the determination of selenium was examined. The calibration curve was found to be linear between the fluorescence quenching (F0 /F) and the concentration of selenium within the range of 0.16-4.00 µg/mL. The detection limit was 1.96 ng/mL and the relative standard deviation was 3.14%. This method can be used for the determination of selenium in Se-enriched garlic bulbs with satisfactory results.
authors.affiliation: Department of Chemistry, Zhoukou Normal University, Zhoukou, 466001, China; Department of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.
authors.forename: Ya-Hong
authors.initials: YH
authors.lastname: Chen
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Zhang
authors.forename: Feng-Shou
authors.initials: FS
authors.lastname: Tian
mesh_terms: D002138:Calibration; D002384:Catalysis; D005453:Fluorescence; D005504:Food Analysis / Q000379:methods; D005737:Garlic / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D057230:Limit of Detection; D012643:Selenium / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013816:Thermodynamics; D014443:Tyrosine / Q000737:chemistry
chemical_list: D006454:Hemoglobins; D014443:Tyrosine; D012643:Selenium
keywords: enzyme-catalyzed; fluorescence quenching; selenium