title: Pharmacokinetics of BPA in gliomas with ultrasound induced blood-brain barrier disruption as measured by microdialysis.
abstract: The blood-brain barrier (BBB) can be transiently disrupted by focused ultrasound (FUS) in the presence of microbubbles for targeted drug delivery. Previous studies have illustrated the pharmacokinetics of drug delivery across the BBB after sonication using indirect visualization techniques. In this study, we investigated the in vivo extracellular kinetics of boronophenylalanine-fructose (BPA-f) in glioma-bearing rats with FUS-induced BBB disruption by microdialysis. After simultaneous intravenous administration of BPA and FUS exposure, the boron concentration in the treated brains was quantified by inductively coupled plasma mass spectroscopy. With FUS, the mean peak concentration of BPA-f in the glioma dialysate was 3.6 times greater than without FUS, and the area under the concentration-time curve was 2.1 times greater. This study demonstrates that intracerebral microdialysis can be used to assess local BBB transport profiles of drugs in a sonicated site. Applying microdialysis to the study of metabolism and pharmacokinetics is useful for obtaining selective information within a specific brain site after FUS-induced BBB disruption.
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan; Biophotonics and Molecular Imaging Research Center, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Feng-Yi
authors.initials: FY
authors.lastname: Yang
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Yi-Li
authors.initials: YL
authors.lastname: Lin
authors.affiliation: Nuclear Science and Technology Development Center, National Tsing Hua University, Hsinchu City, Taiwan; Institute of Nuclear Engineering and Science, National Tsing Hua University, Hsinchu City, Taiwan.
authors.forename: Fong-In
authors.initials: FI
authors.lastname: Chou
authors.affiliation: Institute of Nuclear Engineering and Science, National Tsing Hua University, Hsinchu City, Taiwan.
authors.forename: Yu-Chuan
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Institute of Nuclear Engineering and Science, National Tsing Hua University, Hsinchu City, Taiwan.
authors.forename: Yen-Wan
authors.initials: YW
authors.lastname: Hsueh Liu
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Lun-Wei
authors.initials: LW
authors.lastname: Chang
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Hsieh
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000293:injuries*; D001896:Boron Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001932:Brain Neoplasms / Q000378:metabolism*; D016503:Drug Delivery Systems; D005632:Fructose / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D005910:Glioma / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D051381:Rats; D013016:Sound
chemical_list: C089739:4-boronophenylalanine-fructose; D001896:Boron Compounds; D005632:Fructose