title: Functional p53 can modulate the relationship between E2F-1 expression and tumor kinetics in Hodgkin lymphoma.
abstract: E2F-1 is the best-described member of the E2F family of transcriptional factors and is particularly interesting in view of its often opposing roles. Our purpose was to examine the immunohistochemical expression of E2F-1 in Hodgkin lymphoma (HL) and to correlate it with proliferation and apoptosis of the tumor, clinicopathological parameters and patient outcome, as well as with expression of the downstream molecules p53 and p21. The median percentage of E2F-1-expressing Hodgkin Reed-Sternberg (HRS) cells was 80.2%. A significant positive correlation was found between expression of E2F-1 and p53 (p = 0.034). Following stratification of our cases, within the group harboring functional p53, a statistically significant inverse correlation was identified between E2F-1 and Topo IIa (p = 0.019). E2F-1 is up-regulated in the context of HL and its expression is inversely associated with proliferation. It seems that functional p53 can modulate the relationship between E2F-1 expression and tumor kinetics in HL.
authors.affiliation: Laboratory of Histology and Embryology, National and Kapodistrian University of Athens, Medical School , Athens , Greece.
authors.forename: Eleni C
authors.initials: EC
authors.lastname: Georgiadi
authors.forename: Georgios S
authors.initials: GS
authors.lastname: Dimtsas
authors.forename: Theodoros P
authors.initials: TP
authors.lastname: Vassilakopoulos
authors.forename: Gerassimos A
authors.initials: GA
authors.lastname: Pangalis
authors.forename: Christos
authors.initials: C
authors.lastname: Kittas
authors.forename: Ipatia A
authors.initials: IA
authors.lastname: Doussis-Anagnostopoulou
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000378:metabolism; D017209:Apoptosis; D049109:Cell Proliferation; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D050687:E2F1 Transcription Factor / Q000378:metabolism*; D005260:Female; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D007700:Kinetics; D061986:MCF-7 Cells; D008297:Male; D008875:Middle Aged; D016539:Reed-Sternberg Cells / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D000951:Antigens, Neoplasm; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004268:DNA-Binding Proteins; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53; D004250:DNA Topoisomerases, Type II
keywords: E2F-1; Hodgkin lymphoma; p53; tumor kinetics