title: α-Glucosidase inhibitory activity of polyphenols from the burs of Castanea mollissima Blume.
abstract: Polyphenol extracts from the burs of Castanea mollissima Blume (CMPE) exhibited potential antioxidant and hypoglycemic activities. The α-glucosidase inhibitory activities of CMPE were assessed as a means of elucidating the mechanism behind its hypoglycemic activities. In vitro studies showed that CMPE significantly inhibited both yeast α-glucosidase, through a noncompetitive mode with an IC50 of 0.33 μg/mL, and rat intestinal α-glucosidase. In vivo studies revealed that oral administration of CMPE at doses of 600 mg/kg significantly reduced postprandial blood glucose levels by 27.2% in normal rats following sucrose challenges. Gel permeation chromatography revealed that CMPE exhibited typical characteristics of high-molecular-mass polymers with mean (Mn) and weight (Mw) average molecular weights of 35.4 and 50.7 kDa, respectively, and a polydispersity (Mw/Mn) of 1.432. Acid hydrolysis analysis indicated the presence of ellagitannins. These data suggest that CMPE, enriched with ellagitannins, would be an efficacious dietary supplement for diabetes management through the inhibition of alpha-glucosidase.
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhao
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Yin
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Yan
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Shi
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhou
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. yjliubio@163.com.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. machao@bjfu.edu.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D029962:Fagaceae / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000627:therapeutic use*; D059808:Polyphenols / Q000737:chemistry* / Q000627:therapeutic use*; D051381:Rats; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D059808:Polyphenols; D000520:alpha-Glucosidases