title: GST2 is required for nitrogen starvation-induced filamentous growth in Candida albicans.
abstract: Candida albicans, the major human fungal pathogen, undergoes morphological transition from the budding yeast form to filamentous growth in response to nitrogen starvation. In this study, we identified a new function of GST2, whose expression was required for filamentous growth of C. albicans under nitrogen-limiting conditions. The ΔGst2p showed Gst activity and required response to oxidative stress. The Δgst2 mutant displayed predominantly yeast phase growth in low ammonium media. Such morphological defect of Δgst2 mutants was not rescued by overexpression of Mep2p, Cph1p, or Efg1p, but was rescued by either overexpression of a hyperactive RAS1(G13V) allele or through exogenous addition of cyclic AMP. In addition, the Δgst2 mutants had lower levels of RAS1 transcripts than wild-type cells under conditions of nitrogen starvation. These results were consistent with the Ras1-cAMP pathway as a possible downstream target of Gst2p. These findings suggest that Gst2p is a significant component of nitrogen starvation-induced filamentation in C. albicans.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: So-Hyoung
authors.initials: SH
authors.lastname: Lee
authors.forename: Soon-Chun
authors.initials: SC
authors.lastname: Chung
authors.forename: Jongheon
authors.initials: J
authors.lastname: Shin
authors.forename: Ki-Bong
authors.initials: KB
authors.lastname: Oh
mesh_terms: D002176:Candida albicans / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D025301:Hyphae / Q000235:genetics / Q000254:growth & development; D009154:Mutation / Q000235:genetics; D009584:Nitrogen / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics* / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics; D018631:ras Proteins / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D000242:Cyclic AMP; D005982:Glutathione Transferase; D018631:ras Proteins; D009584:Nitrogen