title: Serenoa Repens Induces Growth Arrest, Apoptosis and Inactivation of STAT3 Signaling in Human Glioma Cells.
abstract: Serenoa repens, the extract of berry in Southeastern United States, is one of several phytotherapeutic agents available for the treatment of Benign prostatic hyperplasia (BPH). In this study, we found for the first time that Serenoa repens effectively inhibited the growth of human U87 and U251 glioma cells. Flow cytometry assay showed that Serenoa repens induced apoptosis of U87 and U251 glioma cells in a dose-dependent manner. Also, Serenoa repens increased the expression of cleaved-PARP, Caspase-3 or p27 protein in these two cell lines, respectively. In addition, we found that Serenoa repens down-regulated basal level of phosphorylated form of signal transducer and activator of transcription 3 (STAT 3) in both U87 and U251 glioma cells. Furthermore, it was discovered that a Janus family of tyrosine kinase (JAK) inhibitor AG490 inhibit the growth of human U87 and U251 glioma cells and AG490 enhanced the ability of Serenoa repens to inhibit the growth of U87 and U251 glioma cells as measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. These results indicate that Serenoa repens reduces the growth, causes apoptosis of Glioma cells and inhibits STAT 3 signaling. In addition, it might also be useful for the treatment of individuals with glioma.
authors.affiliation: Department of Neurology, the Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
authors.forename: Tiezhu
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Neurology, the Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Neurology, the Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Neurology, the Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China cheyq_cmu4h@126.com.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Che
authors.affiliation: Department of Neurology, the Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Neurology, the Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lv
authors.affiliation: Department of Neurology, the Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, the Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurology, the Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Hou
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D005910:Glioma / Q000473:pathology*; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D028024:Serenoa; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C040972:saw palmetto extract
keywords: Apoptosis; Glioma; STAT 3; Serenoa repens