title: [The research on the construction and characteristics of recombinant engineering bacteria with multi-epitope of Helicobacter pylori].
abstract: OBJECTIVE
  To construct the multi epitope prokaryotic expression plasmid and appropriate engineering bacteria expressing the multi-epitope fusion protein of urea membrane channel protein (UreI), urease B subunit (UreB) and adhesin (HpaA) of Helicobacter pylori, then study its microbiological characteristics.
  
  
  METHODS
  The target sequence contains multi epitope gene sequence of Helicobacter pylori were designed and synthesized, subsequently; it was subcloned into the expression vector pET28a (+), confirmed by restriction enzyme digestion and DNA sequencing. The fusion protein rIBA was expressed in E. coli Rosseta (DE3) and analyzed by Western blot.
  
  
  RESULTS
  The plasmid of pET28a(+)/IBA was constructed successfully, confirmed by endonuclease digestion and sequence analyze. The expressed rIBA protein with relative molecular mass about 40 x 10(3) and can be detected by Western blot.
  
  
  CONCLUSIONS
  The prokaryotic engineering bacteria expression multi-epitope of the Helicobacter pylori was constructed successfully. The recombinant protein rIBA expressed by the engineering bacteria can be identified by Sydney strain 1 of Helicobacter pylori (H. pylori SS1) specific antibody IgY, which demonstrated that the rIBA has high correlation with H. pylori SS1.
authors.forename: Na-na
authors.initials: NN
authors.lastname: Ding
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Xing
authors.initials: X
authors.lastname: Pan
authors.forename: Yong-jun
authors.initials: YJ
authors.lastname: Zhou
authors.forename: Jian-chun
authors.initials: JC
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.forename: Bao-ning
authors.initials: BN
authors.lastname: Wang
authors.forename: Ming-yuan
authors.initials: MY
authors.lastname: Li
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis*; D000939:Epitopes / Q000096:biosynthesis*; D004926:Escherichia coli; D005818:Genetic Engineering; D016480:Helicobacter pylori; D026901:Membrane Transport Proteins / Q000096:biosynthesis; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014510:Urease / Q000096:biosynthesis
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D026901:Membrane Transport Proteins; D011993:Recombinant Fusion Proteins; C114527:UreI protein, Helicobacter pylori; C095977:adhesin A, Helicobacter; D014510:Urease