title: Isolation and structure elucidation of cystargamide, a lipopeptide from Kitasatospora cystarginea.
abstract: A new lipopeptide, cystargamide (1) was isolated from the fermentation broth of the actinomycete Kitasatospora cystarginea. The bacterial strain was selected from a set of 12 Kitasatospora spp. using a secondary metabolomics approach combining liquid chromatography/high-resolution mass spectrometry (LC-HRMS) with principal component analysis (PCA). Cystargamide (1) was purified by reversed-phase HPLC, and the structure elucidation was achieved by interpreting mass spectrometry and NMR data. Cystargamide (1) contains rare structural features including a 5-hydroxy tryptophan residue and a 2,3-epoxydecanoyl fatty acid group.
authors.affiliation: Department of Chemistry, University of Prince Edward Island , 550 University Avenue, Charlottetown, Prince Edward Island, Canada C1A 4P3.
authors.forename: Krista A
authors.initials: KA
authors.lastname: Gill
authors.forename: Fabrice
authors.initials: F
authors.lastname: Berrué
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Arens
authors.forename: Russell G
authors.initials: RG
authors.lastname: Kerr
mesh_terms: D000190:Actinomyces / Q000737:chemistry*; D002176:Candida albicans / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D016984:Enterococcus faecium / Q000187:drug effects; D005227:Fatty Acids / Q000737:chemistry; D055666:Lipopeptides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D055666:Lipopeptides; C000590855:cystargamide; D014640:Vancomycin