title: Effect of addition of 2% chlorhexidine or 10% doxycycline on antimicrobial activity of biodentine.
abstract: OBJECTIVE
  The purpose of this in vitro study was to determine whether the addition of 2% chlorhexidine gluconate or 10% doxycycline would enhance the antimicrobial activity of Biodentine against Staphylococcus aureus (ATCC-25923), Enterococcus faecalis (ATCC-29212), Candida albicans (ATCC-90028), and Streptococcus mutans (MTCC-497).
  
  
  METHODS
  Three wells of 4 mm diameter and 4 mm depth on each plate were prepared on the agar medium with standardized suspensions of each microorganism. Biodentine powder mixed with 2% chlorhexidine (0.06 g) or 10% doxycycline (0.30 g) in its liquid or liquid alone was placed to fill each well. Plates were incubated at 37°C as required for microbial growth. A blinded, independent observer measured zones of inhibition. The data were analyzed using independent "t" test to compare the differences among the three cement preparations for different micro-organisms.
  
  
  RESULTS
  All Biodentine samples inhibited microbial growth. The highest mean diameters of zone of inhibition for all the micro-organisms were found around Biodentine/chlorhexidine (13.417) followed by Biodentine alone (12.236) and Biodentine/doxycycline (11.25).
  
  
  CONCLUSIONS
  In conclusion, adding 2% chlorhexidine gluconate in liquid of Biodentine enhanced the antimicrobial activity of Biodentine against all the tested micro-organisms except Candida albicans, while addition of 10% doxycycline decreased the antimicrobial activity of Biodentine. The differences were significant statistically (P < 0.05).
authors.affiliation: Department of Conservative Dentistry and Endodontics, Meerut, Uttar Pradesh, India.
authors.forename: Vineeta
authors.initials: V
authors.lastname: Nikhil
authors.affiliation: Department of Microbiology Subharti Dental College, Meerut, Uttar Pradesh, India.
authors.forename: Molly
authors.initials: M
authors.lastname: Madan
authors.affiliation: Department of Microbiology Subharti Dental College, Meerut, Uttar Pradesh, India.
authors.forename: Charu
authors.initials: C
authors.lastname: Agarwal
authors.affiliation: Department of Conservative Dentistry and Endodontics, Meerut, Uttar Pradesh, India.
authors.forename: Navleen
authors.initials: N
authors.lastname: Suri
keywords: Antimicrobial activity; biodentine; candida albicans; chlorhexidine; doxycycline