title: Fission yeast mtr1p regulates interphase microtubule cortical dwell-time.
abstract: The microtubule cytoskeleton plays important roles in cell polarity, motility and division. Microtubules inherently undergo dynamic instability, stochastically switching between phases of growth and shrinkage. In cells, some microtubule-associated proteins (MAPs) and molecular motors can further modulate microtubule dynamics. We present here the fission yeast mtr1(+), a new regulator of microtubule dynamics that appears to be not a MAP or a motor. mtr1-deletion (mtr1Δ) primarily results in longer microtubule dwell-time at the cell tip cortex, suggesting that mtr1p acts directly or indirectly as a destabilizer of microtubules. mtr1p is antagonistic to mal3p, the ortholog of mammalian EB1, which stabilizes microtubules. mal3Δ results in short microtubules, but can be partially rescued by mtr1Δ, as the double mutant mal3Δ mtr1Δ exhibits longer microtubules than mal3Δ single mutant. By sequence homology, mtr1p is predicted to be a component of the ribosomal quality control complex. Intriguingly, deletion of a predicted ribosomal gene, rps1801, also resulted in longer microtubule dwell-time similar to mtr1Δ. The double-mutant mal3Δ rps1801Δ also exhibits longer microtubules than mal3Δ single mutant alone. Our study suggests a possible involvement of mtr1p and the ribosome complex in modulating microtubule dynamics.
authors.affiliation: Institut Curie, Paris 75005, France CNRS, UMR 144, Paris 75005, France.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Carlier-Grynkorn
authors.affiliation: Institut Curie, Paris 75005, France CNRS, UMR 144, Paris 75005, France.
authors.forename: Liang
authors.initials: L
authors.lastname: Ji
authors.affiliation: Institut Curie, Paris 75005, France CNRS, UMR 144, Paris 75005, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Fraisier
authors.affiliation: Institut Curie, Paris 75005, France.
authors.forename: Berangère
authors.initials: B
authors.lastname: Lombard
authors.affiliation: Institut Curie, Paris 75005, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Dingli
authors.affiliation: Institut Curie, Paris 75005, France.
authors.forename: Damarys
authors.initials: D
authors.lastname: Loew
authors.affiliation: Institut Curie, Paris 75005, France CNRS, UMR 144, Paris 75005, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Paoletti
authors.affiliation: Laboratoire CaCyS, FRE AGIM 3405 UJF-CNRS-EPHE-UMPF, La Tronche 38700, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Ronot
authors.affiliation: Institut Curie, Paris 75005, France CNRS, UMR 144, Paris 75005, France Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA phong.tran@curie.fr.
authors.forename: Phong T
authors.initials: PT
authors.lastname: Tran
keywords: Dynamic instability; Microtubule; Ribosome