title: Plant single-cell and single-cell-type metabolomics.
abstract: In conjunction with genomics, transcriptomics, and proteomics, plant metabolomics is providing large data sets that are paving the way towards a comprehensive and holistic understanding of plant growth, development, defense, and productivity. However, dilution effects from organ- and tissue-based sampling of metabolomes have limited our understanding of the intricate regulation of metabolic pathways and networks at the cellular level. Recent advances in metabolomics methodologies, along with the post-genomic expansion of bioinformatics knowledge and functional genomics tools, have allowed the gathering of enriched information on individual cells and single cell types. Here we review progress, current status, opportunities, and challenges presented by single cell-based metabolomics research in plants.
authors.affiliation: Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Biswapriya B
authors.initials: BB
authors.lastname: Misra
authors.affiliation: Department of Biology, Penn State University, 208 Mueller Laboratory, University Park, PA 16802, USA.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Assmann
authors.affiliation: Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA; Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32610, USA. Electronic address: schen@ufl.edu.
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
mesh_terms: D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D059828:Plant Cells / Q000378:metabolism; D035261:Plant Components, Aerial / Q000166:cytology / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D059010:Single-Cell Analysis / Q000379:methods*
keywords: mass spectrometry; metabolomics; single cell; single cell type; systems biology