title: Biosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicum.
abstract: Pinene is a monoterpenes (C10) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. Herein, we have metabolically-engineered Corynebacterium glutamicum, to produce pinene and studied its toxicity in C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), obtained from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS (P. taeda) and GPPS (A. grandis) resulted in 27 μg ± 7 α-pinene g(-1) cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain may increase pinene production.
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5 Seongbuk-gu, Seoul, 136-791, Republic of Korea.
authors.forename: Min-Kyoung
authors.initials: MK
authors.lastname: Kang
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Eom
authors.forename: Yunje
authors.initials: Y
authors.lastname: Kim
authors.forename: Youngsoon
authors.initials: Y
authors.lastname: Um
authors.forename: Han Min
authors.initials: HM
authors.lastname: Woo
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D000081008:Bicyclic Monoterpenes; D048230:Corynebacterium glutamicum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D060847:Metabolic Engineering; D039821:Monoterpenes / Q000378:metabolism* / Q000633:toxicity; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D011993:Recombinant Fusion Proteins; D005947:Glucose; C005451:alpha-pinene