title: Increased biomass production and glycogen accumulation in apcE gene deleted Synechocystis sp. PCC 6803.
abstract: The effect of phycobilisome antenna-truncation in the cyanobacterium Synechocystis sp. PCC 6803 on biomass production and glycogen accumulation have not yet been fully clarified. To investigate these effects here, the apcE gene, which encodes the anchor protein linking the phycobilisome to the thylakoid membrane, was deleted in a glucose tolerant strain of Synechocystis sp. PCC 6803. Biomass production of the apcE-deleted strain under photoautotrophic and atmospheric air conditions was 1.6 times higher than that of strain PCC 6803 (1.32 ± 0.01 versus 0.84 ± 0.07 g cell-dry weight L(-1), respectively) after 15 days of cultivation. In addition, the glycogen content of the apcE-deleted strain (24.2 ± 0.7%) was also higher than that of strain PCC 6803 (11.1 ± 0.3%). Together, these results demonstrate that antenna truncation by deleting the apcE gene was effective for increasing biomass production and glycogen accumulation under photoautotrophic and atmospheric air conditions in Synechocystis sp. PCC 6803.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Hyogo, Japan.
authors.forename: Ancy
authors.initials: A
authors.lastname: Joseph
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Hyogo, Japan ; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, 3-5 Sanbancho, Chiyoda-ku 102-0075, Tokyo, Japan.
authors.forename: Shimpei
authors.initials: S
authors.lastname: Aikawa
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Hyogo, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita 565-0871, Osaka, Japan ; RIKEN Biomass Engineering Program, 1-7-22 Suehirocho, Tsurumi, Yokohama 230-0045, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuda
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Hyogo, Japan ; Precursory Research for Embryotic Science and Technology (PRESTO), Japan Science and Technology Agency, 3-5 Sanbancho, Chiyoda-ku 102-0075, Tokyo, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Hasunuma
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Hyogo, Japan ; RIKEN Biomass Engineering Program, 1-7-22 Suehirocho, Tsurumi, Yokohama 230-0045, Japan ; Department of Food Bioscience and Technology, College of Life Science and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
keywords: Antenna truncation; Biomass production; Cyanobacteria; Glycogen; Synechocystis sp. PCC 6803