title: Uukuniemi Phlebovirus assembly and secretion leave a functional imprint on the virion glycome.
abstract: Uukuniemi virus (UUKV) is a model system for investigating the genus Phlebovirus of the Bunyaviridae. We report the UUKV glycome, revealing differential processing of the Gn and Gc virion glycoproteins. Both glycoproteins display poly-N-acetyllactosamines, consistent with virion assembly in the medial Golgi apparatus, whereas oligomannose-type glycans required for DC-SIGN-dependent cellular attachment are predominant on Gc. Local virion structure and the route of viral egress from the cell leave a functional imprint on the phleboviral glycome.
authors.affiliation: Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom Max.Crispin@bioch.ox.ac.uk Thomas.Bowden@strubi.ox.ac.uk.
authors.forename: Max
authors.initials: M
authors.lastname: Crispin
authors.affiliation: Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom School of Life Sciences, University of Warwick, Coventry, United Kingdom.
authors.forename: David J
authors.initials: DJ
authors.lastname: Harvey
authors.affiliation: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.
authors.forename: David
authors.initials: D
authors.lastname: Bitto
authors.affiliation: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.
authors.forename: Steinar
authors.initials: S
authors.lastname: Halldorsson
authors.affiliation: Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom.
authors.forename: Camille
authors.initials: C
authors.lastname: Bonomelli
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, United Kingdom.
authors.forename: Matthew
authors.initials: M
authors.lastname: Edgeworth
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, United Kingdom.
authors.forename: James H
authors.initials: JH
authors.lastname: Scrivens
authors.affiliation: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.
authors.forename: Juha T
authors.initials: JT
authors.lastname: Huiskonen
authors.affiliation: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom Max.Crispin@bioch.ox.ac.uk Thomas.Bowden@strubi.ox.ac.uk.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Bowden
mesh_terms: D005936:Glucans / Q000032:analysis*; D054794:Glycomics; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D018066:Uukuniemi virus / Q000502:physiology*; D014764:Viral Proteins / Q000737:chemistry*; D014771:Virion / Q000737:chemistry*; D019065:Virus Assembly; D057074:Virus Release
chemical_list: D005936:Glucans; D006023:Glycoproteins; D014764:Viral Proteins