title: Chemical analysis and antihyperglycemic activity of an original extract from burdock root (Arctium lappa).
abstract: In the present study, we obtained a dried burdock root extract (DBRE) rich in caffeoylquinic acids derivatives. We performed the chemical characterization of DBRE and explored its antihyperglycemic potential in both in vitro and in vivo experiments. Chemical analysis of DBRE using LC-MS and GC-MS revealed the presence of a great majority of dicaffeoylquinic acid derivatives (75.4%) of which 1,5-di-O-caffeoyl-4-O-maloylquinic acid represents 44% of the extract. In the in vitro experiments, DBRE is able to increase glucose uptake in cultured L6 myocytes and to decrease glucagon-induced glucose output from rat isolated hepatocytes together with a reduction of hepatic glucose 6-phosphatase activity. DBRE did not increase insulin secretion in the INS-1 pancreatic β-cell line. In vivo, DBRE improves glucose tolerance both after intraperitoneal and oral subchronic administration. In conclusion, our data demonstrate that DBRE constitutes an original set of caffeoylquinic acid derivatives displaying antihyperglycemic properties.
authors.affiliation: Université Montpellier I , 4 Boulevard Henri IV, Montpellier, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Tousch
authors.forename: Luc P R
authors.initials: LP
authors.lastname: Bidel
authors.forename: Guillaume
authors.initials: G
authors.lastname: Cazals
authors.forename: Karine
authors.initials: K
authors.lastname: Ferrare
authors.forename: Jeremy
authors.initials: J
authors.lastname: Leroy
authors.forename: Marie
authors.initials: M
authors.lastname: Faucanié
authors.forename: Hugues
authors.initials: H
authors.lastname: Chevassus
authors.forename: Michel
authors.initials: M
authors.lastname: Tournier
authors.forename: Anne-Dominique
authors.initials: AD
authors.lastname: Lajoix
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Azay-Milhau
mesh_terms: D000818:Animals; D031170:Arctium / Q000737:chemistry*; D002460:Cell Line; D005934:Glucagon / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D008297:Male; D032342:Muscle Cells / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; C472707:caffeoylquinic acid; D011801:Quinic Acid; D005934:Glucagon; D005952:Glucose-6-Phosphatase; D005947:Glucose