title: Inactivation of the phosphoglucomutase gene pgm in Paenibacillus polymyxa leads to overproduction of fusaricidin.
abstract: Fusaricidin, a lipodepsipeptide isolated from Paenibacillus polymyxa, has high antimicrobial activity against fungi and Gram-positive bacteria. Through mutagenesis, we obtained two mutant strains, N1U7 and N17U7, which produce 6.2- to 7.9-fold more fusaricidin than their parent strain. Causal mutations were identified by whole-genome sequencing, and the two strains each contained at least eleven point mutations, including four common mutations. A mutation in the PPE04441 gene (pgm), encoding an α-phosphoglucomutase, was found to be an important factor in fusaricidin overproduction by complementation experiments. Null mutation of pgm in the parental strain increased fusaricidin production by 5.2-fold. Increased growth and cell viability in stationary phase, reduced exopolysaccharide production, and increased fusA expression were observed in the pgm mutant strains, which might be related to fusaricidin overproduction. This is the first report revealing that PGM deficiency leads to an overproduction of fusaricidin.
authors.affiliation: Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea.
authors.forename: Ha-Rim
authors.initials: HR
authors.lastname: Kim
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Seong-Bin
authors.initials: SB
authors.lastname: Kim
authors.forename: Haeyoung
authors.initials: H
authors.lastname: Jeong
authors.forename: Soo-Keun
authors.initials: SK
authors.lastname: Choi
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Park
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D047630:Depsipeptides / Q000096:biosynthesis*; D020868:Gene Silencing; D009154:Mutation; D056507:Paenibacillus / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D010733:Phosphoglucomutase / Q000235:genetics* / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D047630:Depsipeptides; D010733:Phosphoglucomutase