title: Fabry-Perot-based Fourier-transform hyperspectral imaging allows multi-labeled fluorescence analysis.
abstract: We demonstrate the ability of our hyperspectral imaging device, based on a scanning Fabry-Perot interferometer, to obtain a single hyper-image of a sample marked with different fluorescent molecules, and to unambiguously discriminate them by observing their spectral fingerprints. An experiment carried out with cyanines, fluorescein, and quantum dots emitting in the yellow-orange region, demonstrates the feasibility of multi-labeled fluorescence microscopy without the use of multiple filter sets or dispersive means.
authors.forename: Marco
authors.initials: M
authors.lastname: Pisani
authors.forename: Massimo
authors.initials: M
authors.lastname: Zucco
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D005583:Fourier Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007368:Interferometry / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D057054:Molecular Imaging / Q000295:instrumentation*
chemical_list: D005456:Fluorescent Dyes