title: Comparative evaluation of antibacterial activity of total-etch and self-etch adhesive systems: An ex vitro study.
abstract: OBJECTIVE
  The aim of this ex vivo study was to compare the antibacterial activity of total-etch and self-etch adhesive systems against Streptococcus mutans, Lactobacillus acidophilus, and Actinomyces viscosus through disk diffusion method.
  
  
  METHODS
  The antibacterial effects of Single Bond (SB) and Adper Prompt (AP) and aqueous solution of chlorhexidine 0.2% (positive control) were tested against standard strain of S. mutans, L. acidophilus, and A. viscosus using the disk diffusion method. The diameters of inhibition zones were measured in millimeters. Data was analyzed using Kruskal-Wallis test. Mann-Whitney U test was used for pairwise comparison.
  
  
  RESULTS
  Of all the materials tested, AP showed the maximum inhibitory action against S. mutans and L. acidophilus. Aqueous solution of chlorhexidine 0.2% showed the maximum inhibitory action against A. viscosus. Very minimal antibacterial effect was noted for SB.
  
  
  CONCLUSIONS
  The antibacterial effects observed for the tested different dentin bonding systems may be related to the acidic nature of the materials.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Yenepoya University, Mangalore, Karnataka, India.
authors.forename: Swathi
authors.initials: S
authors.lastname: Amin
authors.affiliation: Department of Conservative Dentistry and Endodontics, Yenepoya University, Mangalore, Karnataka, India.
authors.forename: Harish K
authors.initials: HK
authors.lastname: Shetty
authors.affiliation: Senior Professor & Head, Department of Conservative Dentistry, Royal Dental College, Chalissery, Palakkad, Kerala, India.
authors.forename: Ravi K
authors.initials: RK
authors.lastname: Varma
authors.affiliation: Department of Orthodontics, Yenepoya Dental College, Yenepoya University, Mangalore, Karnataka, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Amin
authors.affiliation: Department of Conservative Dentistry and Endodontics, Yenepoya University, Mangalore, Karnataka, India.
authors.forename: Prathap M S
authors.initials: PM
authors.lastname: Nair
keywords: Antibacterial activity; actinomyces viscosus; dentin bonding systems; lactobacillus acidophilus; streptococcus mutans