title: Association of matrix metalloproteinase (MMP)-2 and -9 expression with extra-gastrointestinal stromal tumor metastasis.
abstract: Matrix metalloproteinase (MMP)-2 and MMP-9 are important proteases involved in invasion and metastasis of various tumors. Extra-gastrointestinal stromal tumors (EGISTs) are rare neoplasms. This study was performed to assess MMP-2 and MMP-9 expression in EGIST tissue samples for association with clinicopathological data from the patients. Twenty-one surgical EGIST tissue specimens were collected for analysis of MMP-2 and MMP- 9 expression using immunohistochemistry. MMP-2 and MMP-9 proteins were expressed in all of the epithelial cell types of EGISTs, whereas they were only expressed in 75% of the spindle cell type, although there was no statistically significant difference (p>0.05). Expression of MMP-2 and MMP-9 proteins was associated with tumor size, mitotic rate, tumor necrosis, and distant metastasis (p<0.05). MMP-2 expression was linked with MMP-9 levels (p<0.05). However, there was no correlation between MMP-9 expression and age, sex, primary site, or cell morphology in any of these 21 EGIST patients (p>0.05). Moreover, expression of MMP-2 and MMP-9 proteins increased with the degree of EGIST risk. This study provided evidence of an association of MMP-2 and MMP-9 expression with advanced EGIST behavior.
authors.affiliation: Pathological Diagnosis Centre, The First Hospital Affiliated to Bethune Medical College, Jilin University, Changchun, Jilin, China E-mail : xmduan@jlu.edu.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Hong-Xi
authors.initials: HX
authors.lastname: Ma
authors.forename: Mei-Shan
authors.initials: MS
authors.lastname: Jin
authors.forename: Ya-Bin
authors.initials: YB
authors.lastname: Zou
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Teng
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Tian
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Wang
authors.forename: Yin-Ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Duan
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D046152:Gastrointestinal Stromal Tumors / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009362:Neoplasm Metastasis / Q000473:pathology*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9