title: Plant growth-promoting bacteria facilitate the growth of barley and oats in salt-impacted soil: implications for phytoremediation of saline soils.
abstract: Plant growth-promoting bacteria (PGPB) strains that contain the enzyme 1-amino-cyclopropane-1-carboxylate (ACC) deaminase can lower stress ethylene levels and improve plant growth. In this study, ACC deaminase-producing bacteria were isolated from a ) salt-impacted ( 50 dS/m) farm field, and their ability to promote plant growth of barley 1): and oats in saline soil was investigated in pouch assays (1% NaCI), greenhouse trials (9.4 dS/m), and field trials (6-24 dS/m). A mix of previously isolated PGPB strains UW3 (Pseudomonas sp.) and UW4 (P. sp.) was also tested for comparison. Rhizobacterial isolate CMH3 (P. corrugata) and UW3+UW4 partially alleviated plant salt stress in growth pouch assays. In greenhouse trials, CMH3 enhanced root biomass of barley and oats by 200% and 50%, respectively. UW3+UW4, CMH3 and isolate CMH2 also enhanced barley and oat shoot growth by 100%-150%. In field tests, shoot biomass of oats tripled when treated with UW3+UW4 and doubled with CHM3 compared with that of untreated plants. PGPB treatment did not affect salt uptake on a per mass basis; higher plant biomass led to greater salt uptake, resulting in decreased soil salinity. This study demonstrates a method for improving plant growth in marginal saline soils. Associated implications for salt
authors.forename: Pearl
authors.initials: P
authors.lastname: Chang
authors.forename: Karen E
authors.initials: KE
authors.lastname: Gerhardt
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Huang
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Yu
authors.forename: Bernard R
authors.initials: BR
authors.lastname: Glick
authors.forename: Perry D
authors.initials: PD
authors.lastname: Gerwing
authors.forename: Bruce M
authors.initials: BM
authors.lastname: Greenberg
mesh_terms: D018554:Avena / Q000254:growth & development / Q000382:microbiology* / Q000502:physiology; D001426:Bacterial Proteins / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D019755:Carbon-Carbon Lyases / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism; D001467:Hordeum / Q000254:growth & development / Q000382:microbiology* / Q000502:physiology; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000502:physiology; D018520:Plant Shoots / Q000254:growth & development / Q000502:physiology; D011549:Pseudomonas / Q000201:enzymology / Q000302:isolation & purification / Q000502:physiology*; D054712:Salinity; D012639:Seeds / Q000382:microbiology / Q000502:physiology; D012965:Sodium Chloride / Q000032:analysis / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D005030:Ethylenes; D010937:Plant Growth Regulators; D012987:Soil; D012965:Sodium Chloride; C036216:ethylene; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases