title: [Nitrate removal by a strain of nitrate-dependent Fe (II) -oxidizing bacteria].
abstract: A nitrate-dependent Fe(II)-oxidizing bacterial strain, named W5, was isolated from the sediment of the East Lake in Wuhan. Strain W5 was studied for its characteristics of denitrification and nitrogen removal. According to its physiological and biochemical characteristics and the analysis of its 16S rRNA gene sequence, strain W5 was identified as Microbacterium sp. The optimal denitrification performance can be obtained under conditions of NO3(-) -N 40 mg x L(-1), Fe2+ 500 mg x L(-1) and pH 6.8-7.0. After one week of cultivation under optimal conditions, nitrate removal percentage reached 87.0%. During the process of the culture, the nitrite nitrogen concentration was no more than 0.31 mg x L(-1) and there was no ammonia nitrogen production. It was indicated that the nitrate was mostly converted into N2. The consumption rate of Fe2+ was 95.2%.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Wang
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Ji
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Yu-Chong
authors.initials: YC
authors.lastname: Sun
authors.forename: Jun
authors.initials: J
authors.lastname: Tian
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D058440:Denitrification; D005296:Ferrous Compounds / Q000737:chemistry*; D009566:Nitrates / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D005296:Ferrous Compounds; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D009584:Nitrogen