title: In Saccharomyces cerevisiae fructose-1,6-bisphosphate contributes to the Crabtree effect through closure of the mitochondrial unspecific channel.
abstract: In Saccharomyces cerevisiae addition of glucose inhibits oxygen consumption, i.e. S. cerevisiae is Crabtree-positive. During active glycolysis hexoses-phosphate accumulate, and probably interact with mitochondria. In an effort to understand the mechanism underlying the Crabtree effect, the effect of two glycolysis-derived hexoses-phosphate was tested on the S. cerevisiae mitochondrial unspecific channel (ScMUC). Glucose-6-phosphate (G6P) promoted partial opening of ScMUC, which led to proton leakage and uncoupling which in turn resulted in, accelerated oxygen consumption. In contrast, fructose-1,6-bisphosphate (F1,6BP) closed ScMUC and thus inhibited the rate of oxygen consumption. When added together, F1,6BP reverted the mild G6P-induced effects. F1,6BP is proposed to be an important modulator of ScMUC, whose closure contributes to the "Crabtree effect".
authors.affiliation: Department of Molecular Genetics, Inst. de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Mónica
authors.initials: M
authors.lastname: Rosas-Lemus
authors.affiliation: Department of Molecular Genetics, Inst. de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Cristina
authors.initials: C
authors.lastname: Uribe-Alvarez
authors.affiliation: Department of Molecular Genetics, Inst. de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Natalia
authors.initials: N
authors.lastname: Chiquete-Félix
authors.affiliation: Department of Molecular Genetics, Inst. de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico. Electronic address: suribe@ifc.unam.mx.
authors.forename: Salvador
authors.initials: S
authors.lastname: Uribe-Carvajal
mesh_terms: D005635:Fructosediphosphates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000378:metabolism; D006019:Glycolysis; D015640:Ion Channel Gating; D053078:Membrane Potential, Mitochondrial; D008933:Mitochondrial Swelling; D010101:Oxygen Consumption; D015221:Potassium Channels / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D005635:Fructosediphosphates; D015221:Potassium Channels; D029701:Saccharomyces cerevisiae Proteins; C477372:YMUC potassium channel protein, S cerevisiae; D019298:Glucose-6-Phosphate; D005947:Glucose; C029063:fructose-1,6-diphosphate
keywords: Crabtree effect; Fructose-1,6-bisphosphate; Glucose-6-phosphate; Mitochondria; Permeability transition; Saccharomyces cerevisiae