title: Proteomic analysis to examine the role of matrix proteins in a gouty tophus from a patient with recurrent gout.
abstract: To examine the role of matrix proteins in the formation of gouty tophus, we analyzed the crystalline components and matrix proteins in a gouty tophus from a patient with recurrent gout. Micro-area X-ray diffraction analysis and infrared spectroscopy indicated that the tophus was composed of monosodium urate monohydrate. Proteomic analysis identified 134 proteins from the tophus as matrix proteins. Many proteins relevant to inflammation and host defense were identified, and immunoglobulin was detected in all four extracted fractions (KCl, formic acid, guanidine-HCl, and ethylenediaminetetraacetic acid) and from many spots throughout a broad molecular weight range after electrophoresis. It is thought that the process of biological defense including the immunity has occurred in the gouty tophus.
authors.affiliation: a Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma Sciences , Teikyo University , Tokyo , Japan.
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Kaneko
authors.forename: Hiro
authors.initials: H
authors.lastname: Iwamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Yasuda
authors.forename: Katsunori
authors.initials: K
authors.lastname: Inazawa
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamaoka
authors.forename: Tomoko
authors.initials: T
authors.lastname: Fukuuchi
authors.forename: Yoshifuru
authors.initials: Y
authors.lastname: Tamura
authors.forename: Shunya
authors.initials: S
authors.lastname: Uchida
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Mawatari
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nakagomi
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Yamada
authors.forename: Shin
authors.initials: S
authors.lastname: Fujimori
mesh_terms: D000368:Aged; D006073:Gout / Q000150:complications* / Q000378:metabolism*; D006801:Humans; D033461:Hyperuricemia / Q000150:complications*; D008297:Male; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D012008:Recurrence
chemical_list: D011506:Proteins
keywords: Gouty tophus; X-ray diffraction; crystalline component; matrix protein; proteomic analysis