title: Activity of AMP-regulated protein kinase and AMP-deaminase in the heart of mice fed high-fat diet.
abstract: AMP-regulated protein kinase (AMPK) is involved in numerous regulatory processes and its role in control of cardiac energy metabolism is particularly important. This activity could be affected by AMP-deaminase (AMPD) since substrate of AMPD is AMPK activator. Hearts of male mouse, fed for six weeks with normal or high-fat diet, were fractionated to enrich AMPK activity. Purified fraction was incubated with AMARA peptide for up to 5 minutes and then conversion of AMARA to pAMARA was determined by liquid chromatography-mass spectrometry (LC/MS) using mass detector. Activity of AMPK in heart was 0.038±0.012 pmol/min/mg protein for mice fed high-fat diet and that was not different to control (0.032±0.01 pmol/min/mg protein). We observed change in AMPD activity. It was 5.39±1.5 nmol/mg tissue/min in heart of mice fed high-fat diet while in heart of mice fed low-fat diet it was 2.29±0.32 nmol/mg tissue/min. Data we present indicate that while total AMPK activity is not changed decrease in AMPD activity may affect AMPK signaling in diabetic heart.
authors.affiliation: a Department of Biochemistry and Clinical Physiology , Medical University of Gdansk , Gdansk , Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Rybakowska
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Slominska
authors.forename: Paweł
authors.initials: P
authors.lastname: Romaszko
authors.forename: Marcin
authors.initials: M
authors.lastname: Lipiński
authors.forename: Paulina
authors.initials: P
authors.lastname: Zukowska
authors.forename: Ryszard T
authors.initials: RT
authors.lastname: Smolenski
mesh_terms: D000659:AMP Deaminase / Q000378:metabolism*; D000263:Adenylate Kinase / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects*; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D009206:Myocardium / Q000201:enzymology*
chemical_list: D001786:Blood Glucose; D000263:Adenylate Kinase; D000659:AMP Deaminase
keywords: AMP-deaminase; AMP-regulated protein kinase; diabetes; high-fat diet