title: Optimization of triacetylfusarinine C and ferricrocin productions in Aspergillus fumigatus.
abstract: Iron is an essential element for all microorganisms. Bacteria and fungi produce versatile siderophores for binding and storing this essential transition metal when its availability is limited in the environment. The aim of the study was to optimize the fermentation medium of Aspergillus fumigatus for siderophore production. Triacetyl-fusarinine C and ferricrocin yields were dependent on glucose and glycine supplementations as well as the initial pH of the culture media. The optimal fermentation medium for triacetylfusarinine C production contained 8% glucose, 0.4% glycine and the initial pH was set to 5.9. Meanwhile, maximal ferricrocin yields were recorded in the presence of 10% glucose, 0.5% glycine and at an initial pH of 7.4. Under optimized fermentation conditions, the yields for triacetylfusarinine C and ferricrocin increased up to 2.9 g/l culture medium and 18.9 mg/g mycelium, respectively.
authors.affiliation: University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology Debrecen Hungary.
authors.forename: Zsuzsa M
authors.initials: ZM
authors.lastname: Szigeti
authors.affiliation: University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology Debrecen Hungary.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Szaniszló
authors.affiliation: University of Debrecen Department of Inorganic and Analytical Chemistry, Faculty of Science and Technology Debrecen Hungary.
authors.forename: Erika
authors.initials: E
authors.lastname: Fazekas
authors.affiliation: University of Debrecen Department of Inorganic and Analytical Chemistry, Faculty of Science and Technology Debrecen Hungary.
authors.forename: Gyöngyi
authors.initials: G
authors.lastname: Gyémánt
authors.affiliation: University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology Debrecen Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Szabon
authors.affiliation: Eszterházy Károly College Department of Zoology, Faculty of Sciences Eger Hungary.
authors.forename: Károly
authors.initials: K
authors.lastname: Antal
authors.affiliation: University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology Debrecen Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Emri
authors.affiliation: University of Debrecen Department of Nephrology, Medical and Health Science Center Debrecen Hungary Hungarian Academy of Sciences Vascular Biology, Thrombosis and Hemostasis Research Group Debrecen Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Balla
authors.affiliation: Hungarian Academy of Sciences Vascular Biology, Thrombosis and Hemostasis Research Group Debrecen Hungary University of Debrecen Department of Neonatology, Medical and Health Science Center Debrecen Hungary.
authors.forename: György
authors.initials: G
authors.lastname: Balla
authors.affiliation: University of Debrecen Department of Physiology, Medical and Health Science Center Debrecen Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Csernoch
authors.affiliation: University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology Debrecen Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
mesh_terms: D001232:Aspergillus fumigatus / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005163:Factor Analysis, Statistical; D005285:Fermentation; D005290:Ferric Compounds / Q000378:metabolism*; D005291:Ferrichrome / Q000031:analogs & derivatives* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006877:Hydroxamic Acids / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D017262:Siderophores / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D005290:Ferric Compounds; D006877:Hydroxamic Acids; D017262:Siderophores; D005291:Ferrichrome; C021678:ferricrocin; C053324:N,N',N''-triacetylfusarinine C; D007501:Iron; D005947:Glucose; D005998:Glycine
keywords: Aspergillus fumigatus Af293; ferricrocin; response surface methodology; triacetylfusarinine C