title: Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag.
abstract: Easy-to-perform and low-cost protein purification methods are in high demand for the mass production of commonly used enzymes that play an important role in bioeconomy. A low-cost and rapid recombinant protein purification system was developed using CBM3 (family 3 cellulose-binding module) as affinity tag. This protocol describes the purification of CBM3-fusion protein and tag-free protein expressed in Pichia pastoris using CBM3 as an affinity tag.
authors.affiliation: School of Life Science, University of Science and Technology of China, Huangshan Road 443, 230026, Hefei, Anhui, People's Republic of China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.forename: Jiong
authors.initials: J
authors.lastname: Hong
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli; D008967:Molecular Biology / Q000379:methods*; D010843:Pichia / Q000737:chemistry; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011994:Recombinant Proteins; D002482:Cellulose; C109691:microcrystalline cellulose