title: Cell pairing using microwell array electrodes based on dielectrophoresis.
abstract: We report a simple device with an array of 10,000 (100 × 100) microwells for producing vertical pairs of cells in individual microwells with a rapid manipulation based on positive dielectrophoresis (p-DEP). The areas encircled with micropoles which fabricated from an electrical insulating photosensitive polymer were used as microwells. The width (14 μm) and depth (25 μm) of the individual microwells restricted the size to two vertically aligned cells. The DEP device for the manipulation of cells consisted of a microfluidic channel with an upper indium tin oxide (ITO) electrode and a lower microwell array electrode fabricated on an ITO substrate. Mouse myeloma cells stained in green were trapped within 1 s in the microwells by p-DEP by applying an alternating current voltage between the upper ITO and the lower microwell array electrode. The cells were retained inside the wells even after switching off the voltage and washing with a fluidic flow. Other myeloma cells stained in blue were then trapped in the microwells occupied by the cells stained in green to form the vertical cell pairing in the microwells. Cells stained in different colors were paired within only 1 min and a pairing efficiency of over 50% was achieved.
authors.affiliation: Graduate School of Material Science, University of Hyogo , 3-2-1 Kouto, Kamigori, Ako, Hyogo 678-1297, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tomita
authors.forename: Fumio
authors.initials: F
authors.lastname: Mizutani
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yasukawa
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000737:chemistry; D002470:Cell Survival; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes; D051379:Mice; D046210:Microfluidic Analytical Techniques; D008856:Microscopy, Fluorescence; D009101:Multiple Myeloma / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods; D013388:Succinimides / Q000737:chemistry; D017971:Tin Compounds
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D001562:Benzimidazoles; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013388:Succinimides; D017971:Tin Compounds; C109984:indium tin oxide; C017807:bisbenzimide ethoxide trihydrochloride