title: Association of serum paraoxonase enzyme activity and oxidative stress markers with dyslipidemia in obese adolescents.
abstract: OBJECTIVE
  The aim of the present study was to investigate the serum paraoxonase 1 (PON1) concentration and oxidative stress markers and assess its relations with the biochemical parameters in obese adolescents.
  
  
  METHODS
  One hundred and fifty obese adolescents (range 16-18 years) and 150 healthy age- and sex-matched controls were enrolled in the study. The data were extracted from a project entitled "Obesity among Youth: Lifestyle and Genetic Factors" funded by the Science and Technology Development Fund, Egypt. Serum paraoxonase 1 (PON1), nitric oxide (NO), and malonaldehyde were measured. Anthropometry, fasting glucose, insulin concentrations, total cholesterol, high density lipoprotein-cholesterol, low density lipoprotein-cholesterol, triglycerides, systolic and diastolic blood pressure (BP) were measured. Insulin resistance was determined by Homeostasis Model Assessment of Insulin Resistance (HOMA-IR). Diagnostic accuracy of oxidative markers to identify dyslipidemia was calculated with ROC analysis.
  
  
  RESULTS
  The study showed that PON1 activity was significantly lower in obese adolescents than controls. Obese adolescents had significant lower NO level and significant increased MA values as compared to controls. PON1 was negatively correlated with MAD and body mass index in obese subjects. Obese adolescents showed dyslipidemia and increased blood pressure and HOMA-IR values. PON1 had high area under the curve in ROC analysis for identifying dyslipidemia in obese subjects.
  
  
  CONCLUSIONS
  Our results indicate that obese subjects have increased oxidative stress and decreased PON1 activity. The lower paraoxonase level might contribute to the greater risk of dyslipidemia, insulin resistance, high blood pressure that are considered as important components in the pathogenesis of the metabolic syndrome in obese adolescents.
authors.affiliation: Department of Biological Anthropology, National Research Center, Cairo, Egypt.
authors.forename: Moushira Erfan
authors.initials: ME
authors.lastname: Zaki
authors.affiliation: Department of Clinical Genetics, National Research Center, Cairo, Egypt.
authors.forename: Hala
authors.initials: H
authors.lastname: El-Bassyouni
authors.affiliation: Department of Biological Anthropology, National Research Center, Cairo, Egypt.
authors.forename: Sanaa
authors.initials: S
authors.lastname: Kamal
authors.affiliation: Department of Clinical Genetics, National Research Center, Cairo, Egypt.
authors.forename: Mona
authors.initials: M
authors.lastname: El-Gammal
authors.affiliation: Department of Medical Biochemistry, National Research Center, Cairo, Egypt.
authors.forename: Eman
authors.initials: E
authors.lastname: Youness
keywords: Dyslipidemia; obesity; oxidative stress; paraoxonase activity