title: Intra-population chemical polymorphism in Thymus pannonicus All. growing in Slovakia.
abstract: Solid-phase microextraction technique coupled with gas chromatography-flame ionisation detector and gas chromatography-mass spectrometry was used to investigate the inter- and intra-population chemical polymorphism in volatile constituents of three populations of Thymus pannonicus growing in Slovakia. To have an idea on what really the plant emits under different environmental and physiological conditions, a total of 86 individuals were separately analysed using low extraction temperatures (30°C). Data were analysed using a chemometric approach such as the principal component analysis which revealed a significant intra-population variability with the existence of up to four chemotypes: the most abundant is the p-cymene/γ-terpinene chemotype, followed by the geraniol and linalool chemotypes, and, only in one population, by the γ-muurolene/(E)-caryophyllene chemotype. This differentiation of individual plants inside the populations allows better adaptation to ecological conditions of localities and is probably connected with intrinsic genetic diversity of the species.
authors.affiliation: a School of Pharmacy, University of Camerino , I-62032 Camerino , Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Maggi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Caprioli
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Papa
authors.forename: Gianni
authors.initials: G
authors.lastname: Sagratini
authors.forename: Sauro
authors.initials: S
authors.lastname: Vittori
authors.forename: Vladislav
authors.initials: V
authors.lastname: Kolarčik
authors.forename: Pavol
authors.initials: P
authors.lastname: Mártonfi
mesh_terms: D000080462:Acyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D000081007:Cymenes; D008401:Gas Chromatography-Mass Spectrometry; D039821:Monoterpenes / Q000032:analysis; D009822:Oils, Volatile / Q000032:analysis; D000081223:Polycyclic Sesquiterpenes; D025341:Principal Component Analysis; D012717:Sesquiterpenes / Q000032:analysis; D018154:Slovakia; D052617:Solid Phase Microextraction; D013729:Terpenes / Q000737:chemistry; D046930:Thymus Plant / Q000737:chemistry* / Q000235:genetics*
chemical_list: D000080462:Acyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D000081007:Cymenes; D039821:Monoterpenes; D009822:Oils, Volatile; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; D013729:Terpenes; C007210:4-cymene; C018669:gamma-terpinene; C024714:caryophyllene; C018584:linalool; C007836:geraniol
keywords: GC; PCA; SPME; Thymus pannonicus; chemotypes