title: An optimized method for corticosterone analysis in mouse plasma by ultra-performance liquid chromatography-full-scan high-resolution accurate mass spectrometry.
abstract: A simple method based on liquid chromatography-full-scan high-resolution accurate mass spectrometry (LC-HRMS) using a quadrupole time-of-flight (TOF) mass spectrometry was developed and optimized for corticosterone quantification in mouse plasma. Mouse plasma (100 μL) was extracted with methyl tert-butyl ether using prednisone as internal standard. Separation was performed on a short C18 column using a methanol-water gradient. Full-scan data were acquired in the TOF only mode, and extracted ion chromatograms were generated post-acquisition with the extract masses of the analytes. Enhanced sensitivity and reproducibility were acquired with optimized mass parameters. The calibration range was 8.24-412 ng/mL, and the limit of quantitation was 5.088 g/mL. Accuracy was between -5.9 and 8.6%. The precision of between-run (interday) and within-run (intraday) was within 5.6 and 6.9%, respectively. The LC-HRMS method was applied for plasma samples analysis from the stressed mice with and without ginseng treatment for the stress state estimation.
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Sheng
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China.
authors.forename: Zong-Lin
authors.initials: ZL
authors.lastname: Yang
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China syliu@ciac.jl.cn.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Liu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003345:Corticosterone / Q000097:blood*; D016014:Linear Models; D008297:Male; D051379:Mice; D005894:Panax; D015203:Reproducibility of Results; D012503:Saponins; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D012503:Saponins; D003345:Corticosterone