title: Efficiency of systemic versus intralesional bone marrow-derived stem cells in regeneration of oral mucosa after induction of formocresol induced ulcers in dogs.
abstract: BACKGROUND
  Bone marrow mesenchymal stem cells (BMSCs) are the key to regenerative wound healing. MSCs have spatial memory and respond to local environment. The goal of this study was to evaluate the use of systemic and intralesional transplantation of BMSCs for regeneration of oral mucosa in an in vivo dog model.
  
  
  METHODS
  Transplantation of undifferentiated green fluorescent protein (GFP)-labeled autologous BMSCs systemically, submucosally or vehicle (saline) was injected around the chemically induced oral ulcer in each group of 18 adult dogs. The healing process of the ulcer was monitored clinically and histopathologically. Gene expression of vascular endothelial growth factor (VEGF) and collagen genes was detected in biopsies from all ulcers. One way ANOVA was used to compare between means of the three groups. Results were considered significant at P < 0.05.
  
  
  RESULTS
  Flow cytometric analysis of the MSCs at the passage 3 showed that these cells were negative for CD45 (2.39%). They expressed high levels of CD29 (98.34%). Frozen fluorescence microscopy of sections of the cell-treated oral tissue of all groups indicated that the GFP-transduced implanted cells were integrated within the transplanted tissues. The treatment resulted in dramatic wound edge activation and resurfacing of oral mucosa wound.
  
  
  CONCLUSIONS
  Our results revealed that BMSCs may be labeled with (GFP), in order to know the distribution of these cells after administration, and suggest that intralesional administration is an appropriate procedure to achieve acceptable regeneration of the previously injured oral mucosa more than systemic route.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Future University, New Cairo, Egypt.
authors.forename: Lobna A
authors.initials: LA
authors.lastname: Aly
authors.affiliation: Department of Periodontology, Oral Medicine and Oral Diagnosis, Faculty of Dentistry, Misr University for Science and Technology, Cairo, Egypt.
authors.forename: Hala
authors.initials: H
authors.lastname: El-Menoufy
authors.affiliation: Department of Periodontology, Oral Medicine and Oral Diagnosis, Faculty of Dentistry, Cairo University, Giza, Egypt.
authors.forename: Hesham S
authors.initials: HS
authors.lastname: Sadeq
authors.affiliation: Department of General Histology, Faculty of Dentistry, Future University, New Cairo, Egypt.
authors.forename: Alyaa
authors.initials: A
authors.lastname: Ragae
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Giza, Egypt.
authors.forename: Dina
authors.initials: D
authors.lastname: Sabry
keywords: BMSCs; intralesional; oral ulcer; systemic