title: [Down-regulation effect of TGF-beta1 on expression of VEGF in bladder cancer cell line].
abstract: OBJECTIVE
  To explore the down-regulation effect of transforming growth factor-beta1 (TGF-beta1) on the expression of vascular endothelial growth factor (VEGF) in bladder cancer cell line EJ cell strain.
  
  
  METHODS
  The siRNA expression vectors of TGF-beta1, gene were constructed, the highest inhibition target sequence was screened and selected by real time-PCR and ELISA, and then the vectors were transfected into EJ cells, the expression level of VEGF mRNA was detected by Real time-PCR.
  
  
  RESULTS
  TGF-beta1 targeting expression vectors were successfully constructed, Real time-PCR and ELISA screened the highest inhibition target sequence (the lowest expression level of TGF-beta1 mRNA was 0.92 +/- 0.19; ELISA result was 50.08 +/- 5.85). The relative expression level of VEGF mRNA in TGF-beta1 siRNA group was the lowest, indicating that the expression of VEGF was decreased by silencing TGF-beta1, gene.
  
  
  CONCLUSIONS
  Inhibition of TGF-beta1 gene expression could down regulate the expression of VEGF in bladder neoplasm.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.forename: Ming-xing
authors.initials: MX
authors.lastname: Qiu
mesh_terms: D045744:Cell Line, Tumor; D015536:Down-Regulation; D006801:Humans; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A