title: The influence of serial repitching of Saccharomyces pastorianus on its karyotype and protein profile during the fermentation of gluten-free buckwheat and quinoa wort.
abstract: Gluten-free beer-like beverages from malted buckwheat and quinoa are somehow close to their commercial production, but rather high expenses are expected due to the relatively high price of grain, some technological adaptations of process and the need for external enzyme supplementation during mashing. One of the common and efficient cost reduction measures in the industrial scale is serial repitching of the yeast biomass, which has not been studied for the buckwheat and quinoa wort fermentation before. In that manner we have monitored possible changes in yeast's proteins and chromosomal DNA during eleven serial repitchings of the yeast Saccharomyces pastorianus strain TUM 34/70 for fermentation of the barley, buckwheat and quinoa wort. Karyotypes showed changes in regard to the raw materials used and many responsible candidate proteins are suggested which could cause these differences. Different relative expressions of some protein bands were also linked to the proteins involved in yeast stress response and proteins involved in fermentation performance. Results suggest that serial repitching of the strain TUM 34/70 seems suitable for the production of gluten-free beer-like beverages from buckwheat and quinoa.
authors.affiliation: Slovenian Institute of Hop Research and Brewing, Department of Agrochemistry and Brewing, Cesta Žalskega tabora 2, SI-3310 Žalec, Slovenia. Electronic address: matjaz.dezelak@ihps.si.
authors.forename: Matjaž
authors.initials: M
authors.lastname: Deželak
authors.affiliation: Technische Universität München, Center of Life and Food Sciences Weihenstephan, Chair of Brewing and Beverage Technology, Weihenstephaner Steig 20, D-85350 Freising, Germany. Electronic address: mekmela@yahoo.com.
authors.forename: Mekonnen M
authors.initials: MM
authors.lastname: Gebremariam
authors.affiliation: University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology, Chair of Biotechnology, Microbiology and Food Safety, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia. Electronic address: neza.cadez@bf.uni-lj.si.
authors.forename: Neža
authors.initials: N
authors.lastname: Cadež
authors.affiliation: University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology, Chair of Biotechnology, Microbiology and Food Safety, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia. Electronic address: jure.zupan@bf.uni-lj.si.
authors.forename: Jure
authors.initials: J
authors.lastname: Zupan
authors.affiliation: University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology, Chair of Biotechnology, Microbiology and Food Safety, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia. Electronic address: peter.raspor@bf.uni-lj.si.
authors.forename: Peter
authors.initials: P
authors.lastname: Raspor
authors.affiliation: Technische Universität München, Center of Life and Food Sciences Weihenstephan, Chair of Brewing and Beverage Technology, Weihenstephaner Steig 20, D-85350 Freising, Germany. Electronic address: martin.zarnkow@wzw.tum.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Zarnkow
authors.affiliation: Technische Universität München, Center of Life and Food Sciences Weihenstephan, Chair of Brewing and Beverage Technology, Weihenstephaner Steig 20, D-85350 Freising, Germany. Electronic address: tb@wzw.tum.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Becker
authors.affiliation: Slovenian Institute of Hop Research and Brewing, Department of Agrochemistry and Brewing, Cesta Žalskega tabora 2, SI-3310 Žalec, Slovenia. Electronic address: iztok.kosir@ihps.si.
authors.forename: Iztok Jože
authors.initials: IJ
authors.lastname: Košir
mesh_terms: D001515:Beer / Q000382:microbiology*; D027465:Chenopodium quinoa / Q000378:metabolism*; D019612:Fagopyrum / Q000378:metabolism*; D005285:Fermentation; D005516:Food Microbiology; D005656:Fungal Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D059785:Karyotype; D012440:Saccharomyces / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D005656:Fungal Proteins
keywords: Buckwheat; Gluten-free beverage; Karyotype; Protein profile; Quinoa; Saccharomyces pastorianus TUM 34/70