title: Protocatechualdehyde prevents methylglyoxal-induced mitochondrial dysfunction and AGEs-RAGE axis activation in human lens epithelial cells.
abstract: Methylglyoxal (MGO), a glucose derived dicarbonyl intermediate, is a major precursor of advanced glycation end products (AGEs) which have been linked to the development of diabetic cataract. Protocatechualdehyde (PCA), a phenolic acid compound, is found in the roots of Salvia miltiorrhiza. This study was to investigate the effect of PCA against MGO-induced cytotoxicity in human lens epithelial cells (SRA01/04 cells) and the possible involved molecular mechanism. The results showed that PCA alleviated MGO-induced mitochondrial dysfunction and apoptosis in SRA01/04 cells. Furthermore, PCA was capable of inhibiting MGO-mediated AGEs formation and blocking receptor of AGEs expression in SRA01/04 cells. It is concluded that PCA could be useful in attenuation of MGO-induced cell damage and the possible mechanism is involved in modulating AGEs-receptor of AGEs axis in human lens epithelial cells, which suggests that PCA has a potential protective effect on diabetic cataract.
authors.affiliation: Beijing Key Laboratory of Drug Target Identification, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China; National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China; State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Drug Target Identification, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Yu-Pei
authors.initials: YP
authors.lastname: Han
authors.affiliation: Jiangsu Kanon Pharmaceutical Co., Ltd, Lianyungang, 222047, China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Yu
authors.affiliation: National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China. Electronic address: pxp123@bjmu.edu.cn.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Pu
authors.affiliation: Beijing Key Laboratory of Drug Target Identification, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China; State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. Electronic address: dugh@imm.ac.cn.
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Du
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001547:Benzaldehydes / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology*; D019610:Cytoprotection / Q000187:drug effects; D004249:DNA Damage; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology*; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D011765:Pyruvaldehyde / Q000633:toxicity*; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000378:metabolism*
chemical_list: D001547:Benzaldehydes; D002396:Catechols; D017127:Glycation End Products, Advanced; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; C005581:protocatechualdehyde; D011765:Pyruvaldehyde
keywords: Advanced glycation end products; Diabetic cataract; Human lens epithelial cells; Methylglyoxal; Protocatechualdehyde