title: Photoprotective effect of arctiin against ultraviolet B-induced damage in HaCaT keratinocytes is mediated by microRNA expression changes.
abstract: Human keratinocytes are located in the outermost skin layer and thus particularly vulnerable to ultraviolet B (UVB) radiation exposure. Previous studies have focused on the cellular and molecular perspectives of UVB-induced keratinocyte damage. In the present study, it was demonstrated that pretreatment with the phytochemical arctiin, one of the lignin compounds, protects human HaCaT keratinocytes from UVB-mediated damage. Biochemical assays revealed that UVB-induced cytotoxicity and cell death were significantly reduced in arctiin-pretreated HaCaT cells. In addition, arctiin promoted the wound healing and DNA repair properties of keratinocytes. The photoprotective effects of arctiin were associated with changes in the expression levels of specific microRNAs (miRNAs) in HaCaT cells. A bioinformatics analysis demonstrated that the miRNAs were functionally involved in cancer, cell cycle, and Wnt and mitogen-activated protein kinase signaling pathways. In the present study, the results from the cellular and molecular assays demonstrated a novel role for arctiin in UVB protection in keratinocytes, which is mediated by miRNA responses and the suppression of UVB-induced cell death. Furthermore, arctiin is implicated as a potential chemopreventive agent through UVB protection of keratinocytes.
authors.affiliation: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hwa Jun
authors.initials: HJ
authors.lastname: Cha
authors.affiliation: Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea.
authors.forename: Ghang Tai
authors.initials: GT
authors.lastname: Lee
authors.affiliation: Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea.
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea.
authors.forename: Kun Kook
authors.initials: KK
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju, Chungcheongbuk-do 361-763, Republic of Korea.
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.affiliation: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Na Kyeong
authors.initials: NK
authors.lastname: Lee
authors.affiliation: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Soo-Yeon
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Bo Mi
authors.initials: BM
authors.lastname: Lee
authors.affiliation: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: In-Sook
authors.initials: IS
authors.lastname: An
authors.affiliation: Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea.
authors.forename: Hyung Jin
authors.initials: HJ
authors.lastname: Hahn
authors.affiliation: Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea.
authors.forename: Kyu Joong
authors.initials: KJ
authors.lastname: Ahn
authors.affiliation: Department of Chemistry, Hanyang University, Seoul 133-791, Republic of Korea.
authors.forename: Su-Jae
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Sungkwan
authors.initials: S
authors.lastname: An
authors.affiliation: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Seunghee
authors.initials: S
authors.lastname: Bae
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D019295:Computational Biology; D004260:DNA Repair / Q000187:drug effects; D005663:Furans / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*; D060449:Wnt Signaling Pathway; D014945:Wound Healing / Q000187:drug effects
chemical_list: D005663:Furans; D005960:Glucosides; D035683:MicroRNAs; D020011:Protective Agents; D020928:Mitogen-Activated Protein Kinases; C077992:arctiin