title: Phylogenetic characterization of VP6 gene (inner capsid) of porcine rotavirus C collected in Japan.
abstract: Porcine rotavirus C (RVC) has been often detected in sporadic cases or outbreaks of diarrhea in suckling and weaned pigs. Previous surveillance studies using both enzyme-linked immunosorbent assays and reverse-transcription polymerase chain reaction in some countries including Japan and the United States have demonstrated a high prevalence of porcine RVCs. In order to understand the phylogenetic relatedness of RVCs, we performed genetic analysis of VP6 gene encoding inner capsid protein by using 22 porcine RVC strains collected in Japan from 2002 to 2010. Comparative analyses of the VP6 nucleotide and amino acid sequences from these porcine RVCs exhibited lower sequence identities than those from human and bovine RVCs. The phylogenetic analysis of VP6 gene of RVC indicated the presence of seven clusters (tentatively assigned I1-I7) according to host species with cut-off values of 87% at the nucleotide level, and VP6 genes of porcine RVCs were divided into five genotypes. These findings indicate that multiple porcine RVC strains with distinctive genotypes are broadly spreading and circulating among farms in Japan. Our data may provide important insights in understanding evolutionary dynamics of RVCs.
authors.affiliation: Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Japan. Electronic address: tohru_suzuki@affrc.go.jp.
authors.forename: Tohru
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Gifu Prefectural Central Livestock Health and Sanitation Office, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Hasebe
authors.affiliation: Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Miyazaki
authors.affiliation: Dairy Hygiene Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsunemitsu
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics*; D007564:Japan / Q000453:epidemiology; D016366:Open Reading Frames; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C061332:VP6 protein, Rotavirus
keywords: Genetic classification; Multiple genotypes; Porcine rotavirus C; VP6