title: A colorimetric sandwich-type assay for sensitive thrombin detection based on enzyme-linked aptamer assay.
abstract: A colorimetric sandwich-type assay based on enzyme-linked aptamer assay has been developed for the fast and sensitive detection of as low as 25 fM of thrombin with high linearity. Aptamer-immobilized glass was used to capture the target analyte, whereas a second aptamer, functionalized with horseradish peroxidase (HRP), was employed for the conventional 3,5,3',5'-tetramethylbenzidine (TMB)-based colorimetric detection. Without the troublesome antibody requirement of the conventional enzyme-linked immunosorbent assay (ELISA), as low as 25 fM of thrombin could be rapidly and reproducibly detected. This assay has superior, or at least equal, recovery and accuracy to that of conventional antibody-based ELISA.
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Jun Hee
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Yea Seul
authors.initials: YS
authors.lastname: Cho
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Sungmuk
authors.initials: S
authors.lastname: Kang
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Gwan-Ho
authors.initials: GH
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: sshah@khu.ac.kr.
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Hah
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D002417:Cattle; D003124:Colorimetry / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D013917:Thrombin / Q000032:analysis* / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D013917:Thrombin
keywords: Aptamer; ELISA; Signal amplification; Thrombin