title: Different effects of lobeline on neuronal and muscle nicotinic receptors.
abstract: Lobeline is a plant alkaloid known to interact with cholinergic system. The effect of lobeline on neuronal α3β4 receptors expressed in COS cells and muscle embryonic αβγδ receptors naturally expressed in TE671 cells was studied using a patch-clamp technique. Our results show that lobeline inhibited responses to acetylcholine in human embryonic muscle nicotinic receptor in a pseudo-noncompetitive manner. The responses of rat neuronal α3β4 receptors to a low concentration of acetylcholine were potentiated by a mixed occupation mechanism that corresponds to "competitive potentiation". This potentiation turned into voltage-dependent inhibition for α3β4 receptors was activated by a high concentration of acetylcholine.
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Kaniakova
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic; Department of Physiology, Faculty of Science, Charles University, Viničná 7, 120 00 Prague, Czech Republic.
authors.forename: Kristyna
authors.initials: K
authors.lastname: Skrenkova
authors.affiliation: Third Surgical Department, First Faculty of Medicine, Charles University, V Úvalu 84, 150 06 Prague, Czech Republic.
authors.forename: Svatopluk
authors.initials: S
authors.lastname: Adamek
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic; Department of Physiology, Faculty of Science, Charles University, Viničná 7, 120 00 Prague, Czech Republic.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Vyskocil
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic. Electronic address: krusek@biomed.cas.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Krusek
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D006801:Humans; D008120:Lobeline / Q000494:pharmacology*; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018722:Nicotinic Agonists / Q000494:pharmacology*; D009928:Organ Specificity; D051381:Rats; D011978:Receptors, Nicotinic / Q000378:metabolism*
chemical_list: D018722:Nicotinic Agonists; D011978:Receptors, Nicotinic; C419362:nicotinic receptor alpha3beta2; D008120:Lobeline
keywords: Embryonic muscle nicotinic receptor; Lobeline; Neuronal nicotinic receptor alpha3beta4