title: Mapped clone and functional analysis of leaf-color gene Ygl7 in a rice hybrid (Oryza sativa L. ssp. indica).
abstract: Leaf-color is an effective marker to identify the hybridization of rice. Leaf-color related genes function in chloroplast development and the photosynthetic pigment biosynthesis of higher plants. The ygl7 (yellow-green leaf 7) is a mutant with spontaneous yellow-green leaf phenotype across the whole lifespan but with no change to its yield traits. We cloned gene Ygl7 (Os03g59640) which encodes a magnesium-chelatase ChlD protein. Expression of ygl7 turns green-leaves to yellow, whereas RNAi-mediated silence of Ygl7 causes a lethal phenotype of the transgenic plants. This indicates the importance of the gene for rice plant. On the other hand, it corroborates that ygl7 is a non-null mutants. The content of photosynthetic pigment is lower in Ygl7 than the wild type, but its light efficiency was comparatively high. All these results indicated that the mutational YGL7 protein does not cause a complete loss of original function but instead acts as a new protein performing a new function. This new function partially includes its preceding function and possesses an additional feature to promote photosynthesis. Chl1, Ygl98, and Ygl3 are three alleles of the OsChlD gene that have been documented previously. However, mutational sites of OsChlD mutant gene and their encoded protein products were different in the three mutants. The three mutants have suppressed grain output. In our experiment, plant materials of three mutants (ygl7, chl1, and ygl98) all exhibited mutational leaf-color during the whole growth period. This result was somewhat different from previous studies. We used ygl7 as female crossed with chl1 and ygl98, respectively. Both the F1 and F2 generation display yellow-green leaf phenotype with their chlorophyll and carotenoid content falling between the values of their parents. Moreover, we noted an important phenomenon: ygl7-NIL's leaf-color is yellow, not yellowy-green, and this is also true of all back-crossed offspring with ygl7.
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China; Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan, China.
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Deng
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China; State Key Laboratory of Hybrid Rice, Hunan, China.
authors.forename: Hai-qing
authors.initials: HQ
authors.lastname: Zhang
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China; Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: He
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China.
authors.forename: Jin-ling
authors.initials: JL
authors.lastname: Liu
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China.
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Shu
authors.affiliation: College of Plant Preservation, Hunan Agricultural University, Changsha, Hunan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China.
authors.forename: Guo-huai
authors.initials: GH
authors.lastname: Wang
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China; Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; Department of Plant Pathology, Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Guo-liang
authors.initials: GL
authors.lastname: Wang
mesh_terms: D019943:Amino Acid Substitution; D002338:Carotenoids / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis / Q000096:biosynthesis; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003116:Color; D003433:Crosses, Genetic; D005091:Exons / Q000235:genetics; D055785:Gene Knockdown Techniques; D005804:Genes, Lethal; D017343:Genes, Plant; D005808:Genes, Recessive; D005816:Genetic Complementation Test; D007178:Inbreeding; D008190:Lyases / Q000737:chemistry / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D020125:Mutation, Missense; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010788:Photosynthesis / Q000235:genetics*; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified; D017354:Point Mutation; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D034741:RNA, Small Interfering / Q000494:pharmacology; D016415:Sequence Alignment
chemical_list: D010940:Plant Proteins; D021122:Protein Subunits; D034741:RNA, Small Interfering; D002734:Chlorophyll; D002338:Carotenoids; D008190:Lyases; C086788:magnesium chelatase