title: Cycloastragenol, a triterpene aglycone derived from Radix astragali, suppresses the accumulation of cytoplasmic lipid droplet in 3T3-L1 adipocytes.
abstract: Cycloastragenol (CAG), a bioactive triterpenoid sapogenin isolated from the Chinese herbal medicine Radix astragali, was reported to promote the phosphorylation of extracellular signal-regulated protein kinase (ERK). Here we investigated the effect of CAG on adipogenesis. The image-based Nile red staining analyses revealed that CAG dose dependently reduced cytoplasmic lipid droplet in 3T3-L1 adipocytes with the IC50 value of 13.0 μM. Meanwhile, cytotoxicity assay provided evidence that CAG was free of injury on HepG2 cells up to 60 μM. In addition, using calcium mobilization assay, we observed that CAG stimulated calcium influx in 3T3-L1 preadipocytes with a dose dependent trend, the EC50 value was determined as 21.9 μM. There were proofs that elevated intracellular calcium played a vital role in suppressing adipocyte differentiation. The current findings demonstrated that CAG was a potential therapeutic candidate for alleviating obesity and hyperlipidemia.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Shifeng
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Chenxi
authors.initials: C
authors.lastname: Zhai
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Ren
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: HD Biosciences Co., Ltd., Shanghai, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Wu
authors.affiliation: HD Biosciences Co., Ltd., Shanghai, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Sun
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China. Electronic address: lishiyou@big.ac.cn.
authors.forename: Shiyou
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China. Electronic address: yjqiao@263.net.
authors.forename: Yanjiang
authors.initials: Y
authors.lastname: Qiao
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D027883:Astragalus Plant / Q000737:chemistry; D027885:Astragalus propinquus; D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D012502:Sapogenins / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D012502:Sapogenins; C027492:Huang Qi; D002118:Calcium; C061014:cycloastragenol
keywords: 3T3-L1 adipocyte; Adipogenesis; Calcium mobilization; Cycloastragenol; Radix astragali