title: ADAM10 is required for SCF-induced mast cell migration.
abstract: A Disintegrin and Metalloproteinase (ADAM)-10 plays critical roles in neuronal migration and distribution. Recently, ADAM10 deletion was shown to disrupt myelopoiesis. We found that inducible deletion of ADAM10 using Mx1-driven Cre recombinase for a period of three weeks resulted in mast cell hyperplasia in the skin, intestine and spleen. Mast cells express surface ADAM10 in vitro and in vivo, at high levels compared to other immune cells tested. ADAM10 is important for mast cell migration, since ADAM10-deficiency reduced c-Kit-mediated migration. As with some mast cell proteases, ADAM10 expression could be altered by the cytokine microenvironment, being inhibited by IL-10 or TGFβ1, but not by several other T cell-derived cytokines. Collectively these data show that the ADAM10 protease is an important factor in mast cell migration and tissue distribution, and can be manipulated by environmental cues.
authors.affiliation: Department of Biology, Virginia Commonwealth University (VCU), Richmond, VA 23284-2012, United States.
authors.forename: Travis W
authors.initials: TW
authors.lastname: Faber
authors.affiliation: Department of Biology, Virginia Commonwealth University (VCU), Richmond, VA 23284-2012, United States.
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Pullen
authors.affiliation: Department of Biology, Virginia Commonwealth University (VCU), Richmond, VA 23284-2012, United States.
authors.forename: Josephine F A
authors.initials: JF
authors.lastname: Fernando
authors.affiliation: Department of Biology, Virginia Commonwealth University (VCU), Richmond, VA 23284-2012, United States.
authors.forename: Elizabeth Motunrayo
authors.initials: EM
authors.lastname: Kolawole
authors.affiliation: Department of Biology, Virginia Commonwealth University (VCU), Richmond, VA 23284-2012, United States.
authors.forename: Jamie J A
authors.initials: JJ
authors.lastname: McLeod
authors.affiliation: Department of Biology, Virginia Commonwealth University (VCU), Richmond, VA 23284-2012, United States.
authors.forename: Marcela
authors.initials: M
authors.lastname: Taruselli
authors.affiliation: Department of Biology, Virginia Commonwealth University (VCU), Richmond, VA 23284-2012, United States.
authors.forename: Kathryn L
authors.initials: KL
authors.lastname: Williams
authors.affiliation: Department of Biology, Virginia Commonwealth University (VCU), Richmond, VA 23284-2012, United States.
authors.forename: Kevin O
authors.initials: KO
authors.lastname: Rivera
authors.affiliation: Department of Biology, Virginia Commonwealth University (VCU), Richmond, VA 23284-2012, United States.
authors.forename: Brian O
authors.initials: BO
authors.lastname: Barnstein
authors.affiliation: Department of Microbiology and Immunology, Virginia Commonwealth University (VCU), Richmond, VA 23284-2012, United States.
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Conrad
authors.affiliation: Department of Biology, Virginia Commonwealth University (VCU), Richmond, VA 23284-2012, United States. Electronic address: jjryan@vcu.edu.
authors.forename: John J
authors.initials: JJ
authors.lastname: Ryan
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000072197:ADAM10 Protein; D053829:Amyloid Precursor Protein Secretases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D002478:Cells, Cultured; D006965:Hyperplasia / Q000235:genetics; D016753:Interleukin-10 / Q000494:pharmacology; D008407:Mast Cells / Q000502:physiology*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010537:Peritoneum / Q000166:cytology; D034622:RNA Interference; D034741:RNA, Small Interfering; D019089:Stem Cell Factor / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: C556062:IL10 protein, mouse; D008565:Membrane Proteins; D034741:RNA, Small Interfering; D019089:Stem Cell Factor; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; D000072197:ADAM10 Protein; C491234:Adam10 protein, mouse
keywords: ADAM-10; Cytokine; Mast cell; Migration; Stem cell factor