title: In vivo and in vitro metabolism of aspirin eugenol ester in dog by liquid chromatography tandem mass spectrometry.
abstract: Aspirin eugenol ester (AEE) is a promising drug candidate for treatment of inflammation, pain and fever and prevention of cardiovascular diseases with fewer side effects than its precursor, aspirin. Investigation into its metabolic process in target animal species will help to illustrate its mechanism of action and to establish its residual mark compound to formulate its dosage. Six beagle dogs were orally given a dose of 20 mg kg(-1) of AEE and one dog was used to prepare blank liver microsomes. Their liver microsomes were prepared for in vitro study and their plasma and urine were collected for in vivo metabolic analysis using liquid chromatography tandem mass spectrometry. In this study we identified 10 metabolites, M1, M2, M3, M4, M5 in phase I and M6, M7, M8, M9, M10 in phase II. Based on the metabolites of AEE, the pathways of AEE metabolism in dog were demonstrated.
authors.affiliation: Key Laboratory of New Animal Drug Project of Gansu Province and Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Gansu Provincial Engineering Research Center for New Animal Drug, Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS, Lanzhou, 730050, People's Republic of China.
authors.forename: Youming
authors.initials: Y
authors.lastname: Shen
authors.forename: Xiwang
authors.initials: X
authors.lastname: Liu
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yang
authors.forename: Jianyong
authors.initials: J
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Bing
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D001241:Aspirin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D005054:Eugenol / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D005260:Female; D008297:Male; D053858:Metabolic Networks and Pathways; D008862:Microsomes, Liver / Q000737:chemistry* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C575435:aspirin eugenol ester; D005054:Eugenol; D001241:Aspirin
keywords: aspirin eugenol ester; liquid chromatography tandem mass spectrometry; metabolism