title: [Effect of Jiangang Yishen Recipe on high insulin induced cell proliferation of human glomerular mesangial cells and the expression of insulin receptor substrate 1 and phosphatidylinositol-3-kinase].
abstract: OBJECTIVE
  To investigate the effect of Jiangtang Yishen Recipe (JTYSR) on high insulin induced cell proliferation of human glomerular mesangial cells (HMCs) and the expression of insulin receptor substrate 1 (IRS-1) and phosphatidylinositol-3-kinase (PI-3K).
  
  
  METHODS
  HMCs were divided into 4 groups, i.e., the negative control group, the high insulin model group, the JTYSR group, and the LY294002 group. The concentration of insulin, JTYSR, and LY294002 was respectively confirmed by pre-experiment. Different culture solution was respectively added for different groups. RPMI1640 culture solution was added for HMCs in the negative control group, while HMCs in the rest 3 groups were cultured by 100 nmol/L insulin for 24 h. Meanwhile, HMCs from the JTYSR group and the LY294002 group were exposed to 125 mg/L JTYSR and 80 micromol/L LY294002 respectively for further 48 h. The proliferation of HMCs was detected by MTT and flow cytometry. The protein expression of IRS-1 and PI-3K in HMC was detected by immunohistochemical assay and Western blot. Results The proliferation of HMCs induced by high insulin could be significantly lowered, and the protein expression of IRS-1 and PI-3K could be down-regulated in the JTYSR group and the LY294002 group (P <0.01). Compared with the LY294002 group, the protein expression of IRS-1 and PI-3K could be slightly down-regulated in the JTYSR group (P <0.05).
  
  
  CONCLUSIONS
  JTYSR could lower high insulin induced proliferation of HMCs, and its mechanism might be related to insulin signaling pathway.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Wu
authors.forename: Rong
authors.initials: R
authors.lastname: Yu
authors.forename: Xi-Hua
authors.initials: XH
authors.lastname: Cheng
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.forename: Can-Rong
authors.initials: CR
authors.lastname: Wu
authors.forename: Guan-De
authors.initials: GD
authors.lastname: Wei
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002867:Chromones; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism*; D050527:Mesangial Cells / Q000502:physiology*; D009025:Morpholines; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D002867:Chromones; D004365:Drugs, Chinese Herbal; D055504:Insulin Receptor Substrate Proteins; D009025:Morpholines; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D019869:Phosphatidylinositol 3-Kinases; D058539:Phosphatidylinositol 3-Kinase