title: Anti-biofilm activity: a function of Klebsiella pneumoniae capsular polysaccharide.
abstract: Competition and cooperation phenomena occur within highly interactive biofilm communities and several non-biocides molecules produced by microorganisms have been described as impairing biofilm formation. In this study, we investigated the anti-biofilm capacities of an ubiquitous and biofilm producing bacterium, Klebsiella pneumoniae. Cell-free supernatant from K. pneumoniae planktonic cultures showed anti-biofilm effects on most Gram positive bacteria tested but also encompassed some Gram negative bacilli. The anti-biofilm non-bactericidal activity was further investigated on Staphylococcus epidermidis, by determining the biofilm biomass, microscopic observations and agglutination measurement through a magnetic bead-mediated agglutination test. Cell-free extracts from K. pneumoniae biofilm (supernatant and acellular matrix) also showed an influence, although to a lesser extend. Chemical analyses indicated that the active molecule was a high molecular weight polysaccharide composed of five monosaccharides: galactose, glucose, rhamnose, glucuronic acid and glucosamine and the main following sugar linkage residues [→ 2)-α-L-Rhap-(1 →]; [→ 4)-α-L-Rhap-(1 →]; [α-D-Galp-(1 →]; [→ 2,3)-α-D-Galp-(1 →]; [→ 3)-β-D-Galp-(1 →] and, [→ 4)-β-D-GlcAp-(1 →]. Characterization of this molecule indicated that this component was more likely capsular polysaccharide (CPS) and precoating of abiotic surfaces with CPS extracts from different serotypes impaired the bacteria-surface interactions. Thus the CPS of Klebsiella would exhibit a pleiotropic activity during biofilm formation, both stimulating the initial adhesion and maturation steps as previously described, but also repelling potential competitors.
authors.affiliation: Clermont Université, UMR CNRS 6023, Laboratoire Microorganismes: Genome Environnement (LMGE), Université d'Auvergne, Clermont-Ferrand, France.
authors.forename: Marina Dos Santos
authors.initials: Mdos S
authors.lastname: Goncalves
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, Polytech Clermont-Ferrand, Aubière, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Delattre
authors.affiliation: Clermont Université, UMR CNRS 6023, Laboratoire Microorganismes: Genome Environnement (LMGE), Université d'Auvergne, Clermont-Ferrand, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Balestrino
authors.affiliation: Clermont Université, UMR CNRS 6023, Laboratoire Microorganismes: Genome Environnement (LMGE), Université d'Auvergne, Clermont-Ferrand, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Charbonnel
authors.affiliation: Université de Picardie Jules Verne, EA 3900-BioPI Biologie des Plantes et de l'Innovation, IUT d'Amiens (GB), Amiens cedex, France.
authors.forename: Redouan
authors.initials: R
authors.lastname: Elboutachfaiti
authors.affiliation: Laboratoire des Glucides FRE CNRS 3517 - Institut de Chimie de Picardie FR 3085, Université de Picardie Jules Verne, Amiens, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Wadouachi
authors.affiliation: BioFilm Control, Biopôle Clermont-Limagne, Saint-Beauzire, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Badel
authors.affiliation: BioFilm Control, Biopôle Clermont-Limagne, Saint-Beauzire, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Bernardi
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, Polytech Clermont-Ferrand, Aubière, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Michaud
authors.affiliation: Clermont Université, UMR CNRS 6023, Laboratoire Microorganismes: Genome Environnement (LMGE), Université d'Auvergne, Clermont-Ferrand, France.
authors.forename: Christiane
authors.initials: C
authors.lastname: Forestier
mesh_terms: D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D018533:Biomass; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002474:Cell-Free System; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000502:physiology*; D010933:Plankton / Q000187:drug effects; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D066244:Proton Magnetic Resonance Spectroscopy; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000502:physiology
chemical_list: D011135:Polysaccharides, Bacterial