title: FIP200 is involved in murine pseudomonas infection by regulating HMGB1 intracellular translocation.
abstract: BACKGROUND
  FIP200, a critical autophagy initiating protein, can participate in numerous cellular functions including cancer development; however, its functional role in P. aeruginosa infection of alveolar macrophages is unknown.
  
  
  METHODS
  To investigate the role of FIP200 in host defense, we transfected murine alveolar macrophage MH-S cells with FIP200 siRNA. Having confirmed that FIP200 knockdown inhibited PAO1-induced autophagosme formation, we sought to characterize the underlying signaling pathways by immunoblotting. Further, we used fip200 KO mice to study the effects of fip200 deficiency on HMGB1 translocation.
  
  
  RESULTS
  We showed that Pseudomonas PAO1 strain infection facilitated autophagosome formation, whereas knockdown of FIP200 inhibited autophagosome formation and HMGB1 expression in MH-S cells. Silencing FIP200 impaired the translocation of HMGB1 to cytosol of MH-S cells and almost abolished acetylation of HMGB1 during PAO1 infection. In contrast, FIP200 overexpression facilitated the cytosol translocation of HMGB1 from nuclei and increased acetylation of HMGB1 in PAO1-infected MH-S cells. Importantly, expression and acetylation of HMGB1 were also significantly down-regulated in fip200 KO mice following PAO1 infection.
  
  
  CONCLUSIONS
  Collectively, these findings elucidate that FIP200 may regulate expression and translocation of HMGB1 during PAO1 infection, which may indicate novel therapeutic targets to control pulmonary infection.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Chang-pei
authors.initials: CP
authors.lastname: Gan
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Xi-kun
authors.initials: XK
authors.lastname: Zhou
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Li
authors.forename: Yu-quan
authors.initials: YQ
authors.lastname: Wei
authors.forename: Jin-liang
authors.initials: JL
authors.lastname: Yang
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics; D000071183:Autophagy-Related Proteins; D015153:Blotting, Western; D002460:Cell Line; D015870:Gene Expression; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000382:microbiology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D010587:Phagocytosis / Q000235:genetics; D010588:Phagosomes / Q000378:metabolism / Q000382:microbiology; D021381:Protein Transport / Q000235:genetics; D011552:Pseudomonas Infections / Q000378:metabolism* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000071183:Autophagy-Related Proteins; D024243:HMGB1 Protein; D047908:Intracellular Signaling Peptides and Proteins; C428743:Rb1cc1 protein, mouse