title: [The mechanism of ciclosporin A regulating the expression of LOX-1 on vascular endothelium of hyperlipidemic rats].
abstract: OBJECTIVE
  To observe the influence of Ciclosporin A (CsA) on the expression of lectin-like oxidized low-density lipoprotein (ox-LDL) receptor-1 (LOX-1) on vascular endothelium of hyperlipidemic rats.
  
  
  METHODS
  Hyperlipidemic rats model as for the study objects firstly were established. Then the high [5 mg/(kg x d)] and low [1 mg/(kg x d] dose of CsA were used to stimulate these rats, while the rats of normal blood lipids as control. After the end of intervention, the protein expressions of LOX-1 and C-reactive protein (CRP) on vascular endothelium of thoracic aorta were observed by Western blot and immunohistochemistry respectively. The serum levels of ox-LDL and reactive oxygen species (ROS) were also examined.
  
  
  RESULTS
  An obviously up-regulated expression of LOX-1 on vascular endothelium of hyperlipidemic rats treated with CsA was observed (P < 0.05), but not the serum level of ox-LDL (P > 0.05). The further study result implied that CsA could increase the expression of CRP on vascular endothelial cells and serum level of ROS (P<0. 05).
  
  
  CONCLUSIONS
  CsA can significantly upregulate the expression of LOX-1 on vascular endothelium of hyperlipidemic rats through not only ox-LDL/LOX-1 pathway but also ROS/CRP pathway, which could cause the probability of occurrence of atherosclerosis.
authors.forename: Hou-ke
authors.initials: HK
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Bu
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000818:Animals; D050197:Atherosclerosis; D002097:C-Reactive Protein / Q000378:metabolism; D016572:Cyclosporine / Q000494:pharmacology*; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D006949:Hyperlipidemias / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D051127:Scavenger Receptors, Class E / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D017382:Reactive Oxygen Species; D051127:Scavenger Receptors, Class E; D016572:Cyclosporine; D002097:C-Reactive Protein