title: Molecular characterization of sympatrically distributed Neotricula aperta-like snails in the Mekong River, Kratie, Cambodia.
abstract: Fifty-six samples of Neotricula aperta-like snails were collected from six locations in Cambodia. Their mitochondrial cytochrome c oxidase subunit 1 (cox1) sequences were examined using haplotype network and neighbor-joining (NJ) tree analysis. Twenty-seven haplotypes (H1-H27) were observed and were divided into two different groups/lineages. Of 27, 17 haplotypes (H11-H27) were clustered with the reference samples of the γ-race N. aperta. The remaining 10 haplotypes (H1-H10) were clustered in a separate group/lineage, differing from the reference samples of the α-, β-, and γ-race N. aperta, suggesting a new lineage belonging the genus Neotricula. Our results show that both the γ-race and a new lineage were sympatrically present approximately 60 km upstream of the Mekong River near the Kratie port, Cambodia. Further morphological and molecular studies are required to confirm the taxonomic status of this new, unidentified lineage.
authors.affiliation: a Department of Environmental Health Sciences , Kochi Medical School, Kochi University , Kochi , Japan .|b The First Affiliated Hospital Of Jiamusi University, Jiamusi University , Jiamusi , Heilongjiang Province , People's Republic of China .
authors.forename: Chennan
authors.initials: C
authors.lastname: Wang
authors.affiliation: c Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University , Mahasarakham , Thailand .
authors.forename: Weerachai
authors.initials: W
authors.lastname: Saijuntha
authors.affiliation: d Laboratory of Tropical Medicine and Parasitology , Dokkyo Medical University , Mibu, Shimotsuga , Tochigi , Japan , and.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kirinoki
authors.affiliation: d Laboratory of Tropical Medicine and Parasitology , Dokkyo Medical University , Mibu, Shimotsuga , Tochigi , Japan , and.
authors.forename: Naoko
authors.initials: N
authors.lastname: Hayashi
authors.affiliation: d Laboratory of Tropical Medicine and Parasitology , Dokkyo Medical University , Mibu, Shimotsuga , Tochigi , Japan , and.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Chigusa
authors.affiliation: e National Centre for Malaria Control, Parasitology and Entomology , Phnom Penh , Cambodia.
authors.forename: Sinuon
authors.initials: S
authors.lastname: Muth
authors.affiliation: e National Centre for Malaria Control, Parasitology and Entomology , Phnom Penh , Cambodia.
authors.forename: Chuor Char
authors.initials: CC
authors.lastname: Meng
authors.affiliation: b The First Affiliated Hospital Of Jiamusi University, Jiamusi University , Jiamusi , Heilongjiang Province , People's Republic of China .
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Ai
authors.affiliation: a Department of Environmental Health Sciences , Kochi Medical School, Kochi University , Kochi , Japan .
authors.forename: Takeshi
authors.initials: T
authors.lastname: Agatsuma
mesh_terms: D000818:Animals; D002160:Cambodia; D003576:Electron Transport Complex IV / Q000235:genetics*; D006239:Haplotypes; D010802:Phylogeny; D045483:Rivers; D012908:Snails / Q000145:classification* / Q000235:genetics*
chemical_list: D003576:Electron Transport Complex IV
keywords: Cambodia; Kratie; N. aperta-like; Neotricula aperta; schistosomiasis mekongi; unidentified lineage