title: Heterotopic bone formation derived from multipotent stromal cells is not inhibited in aged mice.
abstract: OBJECTIVE
  Decreased bone formation with age is believed to arise, at least in part, because of the influence of the senescent microenvironment. In this context, it is unclear whether multipotent stromal cell (MSC)-based therapies would be effective for the treatment of bone diseases.
  
  
  METHODS
  With the use of a heterotopic bone formation model, we investigated whether MSC-derived osteogenesis is impaired in aged mice compared with young mice.
  
  
  RESULTS
  We found that bone formation derived from MSCs is not reduced in aged mice. These results are supported by the unexpected finding that conditioned media collected from ionizing radiation-induced senescent MSCs can stimulate mineralization and delay osteoclastogenesis in vitro.
  
  
  CONCLUSIONS
  Overall, our results suggest that impaired bone formation with age is mainly cell-autonomous and provide a rationale for the use of MSC-based therapies for the treatment of bone diseases in the elderly.
authors.affiliation: Centre de Recherche du CHU Ste-Justine, Université de Montréal, Montréal, Québec, Canada; Département de Pharmacologie, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Carbonneau
authors.affiliation: Centre de Recherche du CHU Ste-Justine, Université de Montréal, Montréal, Québec, Canada; Département de Pharmacologie, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Despars
authors.affiliation: Centre de Recherche du CHU Ste-Justine, Université de Montréal, Montréal, Québec, Canada; Département de Pharmacologie, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Gaël Moquin
authors.initials: GM
authors.lastname: Beaudry
authors.affiliation: Centre de Recherche du CHU Ste-Justine, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Basma
authors.initials: B
authors.lastname: Benabdallah
authors.affiliation: Centre de Recherche du CHU Ste-Justine, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Saadallah
authors.initials: S
authors.lastname: Bouhanik
authors.affiliation: Centre de Recherche du CHU Ste-Justine, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Josée
authors.initials: J
authors.lastname: Dépôt
authors.affiliation: Centre de Recherche du CHU Ste-Justine, Université de Montréal, Montréal, Québec, Canada; Département de Somatologie, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Alain
authors.initials: A
authors.lastname: Moreau
authors.affiliation: Centre de Recherche du CHU Ste-Justine, Université de Montréal, Montréal, Québec, Canada; Département de Pharmacologie, Université de Montréal, Montréal, Québec, Canada. Electronic address: christian.beausejour@recherche-ste-justine.qc.ca.
authors.forename: Christian M
authors.initials: CM
authors.lastname: Beauséjour
mesh_terms: D000368:Aged; D000375:Aging / Q000473:pathology; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000235:genetics; D064987:Cell- and Tissue-Based Therapy; D017077:Culture Media, Conditioned; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology*; D010012:Osteogenesis
chemical_list: D017077:Culture Media, Conditioned
keywords: MSC; aging; heterotopic bone; ionizing radiation; senescence