title: Aim24 stabilizes respiratory chain supercomplexes and is required for efficient respiration.
abstract: The mitochondrial respiratory chain is essential for the conversion of energy derived from the oxidation of metabolites into the membrane potential, which drives the synthesis of ATP. The electron transporting complexes bc1 complex and the cytochrome c oxidase assemble into large supercomplexes, allowing efficient energy transduction. Currently, we have only limited information about what determines the structure of the supercomplex. Here, we characterize Aim24 in baker's yeast as a protein, which is integrated in the mitochondrial inner membrane and is required for the structural integrity of the supercomplex. Deletion of AIM24 strongly affects activity of the respiratory chain and induces a growth defect on non-fermentable medium. Our data indicate that Aim24 has a function in stabilizing the respiratory chain supercomplexes.
authors.affiliation: Department of Cellular Biochemistry, University of Göttingen, D-37073 Göttingen, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Deckers
authors.affiliation: Department of Cellular Biochemistry, University of Göttingen, D-37073 Göttingen, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Balleininger
authors.affiliation: Department of Cellular Biochemistry, University of Göttingen, D-37073 Göttingen, Germany.
authors.forename: Milena
authors.initials: M
authors.lastname: Vukotic
authors.affiliation: Department of Cellular Biochemistry, University of Göttingen, D-37073 Göttingen, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Römpler
authors.affiliation: Department of Cellular Biochemistry, University of Göttingen, D-37073 Göttingen, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Bareth
authors.affiliation: Department of Cellular Biochemistry, University of Göttingen, D-37073 Göttingen, Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Juris
authors.affiliation: Department of Cellular Biochemistry, University of Göttingen, D-37073 Göttingen, Germany. Electronic address: jan.dudek@med.uni-goettingen.de.
authors.forename: Jan
authors.initials: J
authors.lastname: Dudek
mesh_terms: D000595:Amino Acid Sequence; D003470:Culture Media; D045222:Electron Transport Chain Complex Proteins / Q000737:chemistry* / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism; D008969:Molecular Sequence Data; D055550:Protein Stability; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: C000592376:Aim24 protein, S cerevisiae; D003470:Culture Media; D045222:Electron Transport Chain Complex Proteins; D029701:Saccharomyces cerevisiae Proteins
keywords: Aim24; Membrane protein; Metabolism; Mitochondria; Respiration; Supercomplex