title: Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast.
abstract: The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.
authors.affiliation: Laboratory of Cell Regulation, Cancer Research UK, London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
authors.forename: Ngang Heok
authors.initials: NH
authors.lastname: Tang
authors.affiliation: Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Okada
authors.affiliation: Laboratory of Cell Regulation, Cancer Research UK, London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
authors.forename: Chii Shyang
authors.initials: CS
authors.lastname: Fong
authors.affiliation: Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan; Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Center for Advanced Biomedical Sciences (TWIns), 2-2 Wakamatsucho, Shinjuku, Tokyo 162-8480, Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Arai
authors.affiliation: Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan; Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Center for Advanced Biomedical Sciences (TWIns), 2-2 Wakamatsucho, Shinjuku, Tokyo 162-8480, Japan; PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Sato
authors.affiliation: Laboratory of Cell Regulation, Cancer Research UK, London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3LY, UK. Electronic address: takashi.toda@cancer.org.uk.
authors.forename: Takashi
authors.initials: T
authors.lastname: Toda
mesh_terms: D000595:Amino Acid Sequence; D000941:Antigens / Q000378:metabolism; D018797:Cell Cycle Proteins; D017124:Conserved Sequence; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000378:metabolism*; D008938:Mitosis; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D021381:Protein Transport; D012568:Schizosaccharomyces / Q000166:cytology* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism*; D064048:Spindle Pole Bodies / Q000378:metabolism*
chemical_list: C479626:Alp7 protein, S pombe; D000941:Antigens; D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; C454082:pcp1 protein, S pombe; C086178:pericentrin
keywords: Centrosome; Fission yeast; Pericentrin family; Spindle microtubule; Spindle pole body; TACC