title: Gypenoside attenuates hepatic ischemia/reperfusion injury in mice via anti-oxidative and anti-apoptotic bioactivities.
abstract: Gynostemma pentaphyllumis a traditional Chinese medicine that has previously been used for the treatment of chronic inflammation, hyperlipidemia and liver disease. Gypenoside (GP), the predominant component ofGynostemma pentaphyllum, exhibits a therapeutic effect on chronic hepatic injury, fibrosis and fatty liver disease via its anti-inflammatory and anti-oxidant activity. However, the effect of GP on ischemia/reperfusion (I/R)-induced hepatic injury has, to the best of our knowledge, not previously been investigated. In the present study, a hepatic I/R-injury model was successfully established using C57BL/6 mice. In the treatment group, 50 mg/kg GP was administered orally 1 h prior to ischemia. Following hepatic I/R, the levels of hepatic lipid peroxidation and serum alanine aminotransferase increased, while the ratio of hepatic glutathione (GSH):oxidized GSH was reduced, which was effectively attenuated by pretreatment with GP. Furthermore, an increased protein expression of heme oxygenase-1 in the liver tissues of the I/R mice was attenuated by the administration of GP. In addition, the present study indicated that treatment with GP suppressed the I/R-induced increase in the pro-apoptotic protein levels of Bax and cytochromecand the activity of caspase-3/8, as well as the I/R-induced decrease in the levels of anti-apoptotic protein Bcl-2. In conclusion, the present study indicated that GP effectively protected against I/R-induced hepatic injury via its anti-oxidative and anti-apoptotic bioactivity.
authors.affiliation: Center of Transplant Medicine Engineering and Technology of the Ministry of Health of The People's Republic of China, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Center of Transplant Medicine Engineering and Technology of the Ministry of Health of The People's Republic of China, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Ming
authors.affiliation: Center of Transplant Medicine Engineering and Technology of the Ministry of Health of The People's Republic of China, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Qiquan
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Center of Transplant Medicine Engineering and Technology of the Ministry of Health of The People's Republic of China, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Ye
authors.affiliation: Center of Transplant Medicine Engineering and Technology of the Ministry of Health of The People's Republic of China, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Song
authors.initials: S
authors.lastname: Xie
authors.affiliation: Center of Transplant Medicine Engineering and Technology of the Ministry of Health of The People's Republic of China, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Institute of Hepatobiliary Disease, Transplant Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Hepatobiliary Disease, Transplant Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Zibiao
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Institute of Hepatobiliary Disease, Transplant Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: Center of Transplant Medicine Engineering and Technology of the Ministry of Health of The People's Republic of China, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China ; Institute of Hepatobiliary Disease, Transplant Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Qifa
authors.initials: Q
authors.lastname: Ye
keywords: apoptosis; gypenoside; hepatic injury; ischemia/reperfusion; oxidative damage