title: Reduced Krüppel-like factor 17 (KLF17) expression correlates with poor survival in patients with gastric cancer.
abstract: OBJECTIVE
  To investigate the expression and prognostic significance of Krüppel-like factor 17 (KLF17) in human gastric cancer.
  
  
  METHODS
  KLF17 expressions in 158 paraffin-embedded gastric cancer samples were analyzed using immunohistochemistry. In addition, KLF17 expressions patterns in three fresh gastric cancer tissues and noncancerous gastric mucosa were examined by Western blotting. The correlation between KLF17 expression and clinicopathological factors as well as patient survival was investigated.
  
  
  RESULTS
  Immunohistochemical staining data indicated that KLF17 expression was significantly decreased in 98 of 158 gastric adenocarcinoma cases. Reduced KLF17 expression in fresh gastric cancer tissues was confirmed by Western blotting. Reduced expression of KLF17 was strongly correlated with tumor size, pN stage and lymphovascular invasion. Multivariate Cox regression analysis identified KLF17 expression as an independent prognostic factor for both overall survival (HR = 0.481, 95% CI = 0.225-0.665, p = 0.009) and disease-free survival (HR = 0.438, 95% CI = 0.254-0.758, p = 0.003).
  
  
  CONCLUSIONS
  The reduced expression of KLF17 protein in gastric cancer was correlated with tumor size, pN stage and lymphovascular invasion and was an independent predictor for poor survival in patients undergoing surgery for gastric cancer.
authors.affiliation: Department of Gastroenterology, General Hospital of the Yangtze River Shipping, Wuhan, Hubei Province, China.
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Peng
authors.affiliation: Department of Gastrointestinal Surgery, The First Hospital of Wu Han City, Wuhan, Hubei Province, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Gastrointestinal Surgery, The First Hospital of Wu Han City, Wuhan, Hubei Province, China. Electronic address: xiaoxbsubmission@163.com.
authors.forename: Xin-bo
authors.initials: XB
authors.lastname: Xiao
authors.affiliation: Department of Gastrointestinal Surgery, The First Hospital of Wu Han City, Wuhan, Hubei Province, China.
authors.forename: Yong-sheng
authors.initials: YS
authors.lastname: Shao
authors.affiliation: Department of Pathology, The First Hospital of Wu Han City, Wuhan, Hubei Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Pathology, The First Hospital of Wu Han City, Wuhan, Hubei Province, China.
authors.forename: Meng-xin
authors.initials: MX
authors.lastname: Yin
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D016019:Survival Analysis; D014157:Transcription Factors / Q000378:metabolism*; D047368:Tumor Burden
chemical_list: D014408:Biomarkers, Tumor; C510638:KLF17 protein, human; D014157:Transcription Factors
keywords: Immunohistochemistry; KLF17; Prognosis; Stomach neoplasms; Western blotting