title: Interaction of CD5 and CD72 is involved in regulatory T and B cell homeostasis.
abstract: Regulatory IL-10-producing CD1d(high)CD5(+)CD19(+) B cells and CD4(+)CD25(+)Foxp3(+) T cells have been found to modulate immune responses in autoimmunity, infection, and cancer, but the interaction between these two cell subsets remains unclear. Through cell culture and flow cytometry (FACS), we analyzed the interaction of regulatory T cells (Tregs) and regulatory B cells (Bregs). A neutralizing antibody was used to determine the role of CD5 and CD72 in maintaining regulatory T and B cell homeostasis. We found that CD19(+)CD5(+)CD1d(hi) Bregs induced expansion of CD4(+)Foxp3(+) Tregs, and CD4(+)CD25(+) Tregs also induced expansion of IL-10-expressing Bregs. Once CD72 or CD5 was blocked, both IL-10-expressing Bregs and CD4(+)Foxp3(+)Tregs were reduced in the different cultures. Finally, FACS analysis demonstrated that Foxp3(+)CD4(+)Treg cells were reduced in CD19(Cre) mice defective of CD5 on the surface of B cells. The study suggests that the interaction of CD5 and CD72 plays a critical role in maintaining regulatory T and B cell homeostasis.
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences , Beijing , China .
authors.forename: Mingke
authors.initials: M
authors.lastname: Zheng
authors.forename: Chen
authors.initials: C
authors.lastname: Xing
authors.forename: He
authors.initials: H
authors.lastname: Xiao
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Wang
authors.forename: Gencheng
authors.initials: G
authors.lastname: Han
authors.forename: Guojiang
authors.initials: G
authors.lastname: Chen
authors.forename: Chunmei
authors.initials: C
authors.lastname: Hou
authors.forename: Beifen
authors.initials: B
authors.lastname: Shen
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Renxi
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D060151:B-Lymphocytes, Regulatory / Q000276:immunology*; D018956:CD5 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D006706:Homeostasis; D016753:Interleukin-10 / Q000276:immunology; D008297:Male; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D018956:CD5 Antigens; C513972:CD72 antigen, mouse; C526836:Cd5 protein, mouse; C556062:IL10 protein, mouse; D016753:Interleukin-10
keywords: CD5; CD72; immune homeostasis; regulatory B cells; regulatory T cells