title: Influence of FcγRIIIb polymorphism on its ability to cooperate with FcγRIIa and CR3 in mediating the oxidative burst of human neutrophils.
abstract: Considering that human neutrophil FcγRIIa and FcγRIIIb receptors interact synergistically with CR3 in triggering neutrophil functional responses, allelic polymorphisms in these receptors might influence such interactions. We assessed whether FcγRIIIb polymorphisms affect FcγR/CR cooperation in mediating the neutrophil oxidative burst (OB), in particular the FcγRIIIb/CR3 cooperation that occurs via lectin-saccharide-like interactions. The OB of human neutrophil antigen (HNA)-1a-, HNA-1b-, and HNA-1a/-1b-neutrophils stimulated with immune complexes, opsonized or not with serum complement, was measured by the luminol-enhanced chemiluminescence assay. Compared with HNA-1a-neutrophils, HNA-1b-neutrophils exhibited reduced FcγR-stimulated OB, but increased FcγR/CR-stimulated OB. It suggests that (i) FcγR and CR cooperate more effectively in HNA-1b-neutrophils, and (ii) the HNA-1b allotype influences the FcγRIIIb cooperation with FcγRIIa, but not with CR3. HNA-1a- and HNA-1b-neutrophils exhibited similar OB responses elicited via CR3 alone or via FcγR/CR-independent pathways. In addition, the level of FcγRIIIb, FcγRIIa, and CR3 expression did not differ significantly among the neutrophil groups studied. Together, these results demonstrate that the HNA-1b allotype influences the functional cooperation between FcγRIIIb and FcγRIIa, and suggest that the difference in the glycosylation pattern between HNA-1a and HNA-1b does not affect the FcγRIIIb cooperation with CR3.
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Rua Expedicionários do Brasil, 1621, Centro, Araraquara, SP CEP 14801-360, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Urbaczek
authors.affiliation: Imunologia Básica e Aplicada, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (USP), Avenida Bandeirantes, 3900, Monte Alegre, Ribeirão Preto, SP CEP 14049-900, Brazil.
authors.forename: Juliana Escher
authors.initials: JE
authors.lastname: Toller-Kawahisa
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Rua Expedicionários do Brasil, 1621, Centro, Araraquara, SP CEP 14801-360, Brazil.
authors.forename: Luiz Marcos
authors.initials: LM
authors.lastname: Fonseca
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Rua Expedicionários do Brasil, 1621, Centro, Araraquara, SP CEP 14801-360, Brazil.
authors.forename: Paulo Inácio
authors.initials: PI
authors.lastname: Costa
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Rua Expedicionários do Brasil, 1621, Centro, Araraquara, SP CEP 14801-360, Brazil.
authors.forename: Carolina Maria Quinello Gomes
authors.initials: CM
authors.lastname: Faria
authors.affiliation: Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Avenida do Café, s/n, Monte Alegre, Ribeirão Preto, SP CEP 14040-903, Brazil.
authors.forename: Ana Elisa Caleiro Seixas
authors.initials: AE
authors.lastname: Azzolini
authors.affiliation: Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Avenida do Café, s/n, Monte Alegre, Ribeirão Preto, SP CEP 14040-903, Brazil.
authors.forename: Yara Maria
authors.initials: YM
authors.lastname: Lucisano-Valim
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Avenida do Café, s/n, Monte Alegre, Ribeirão Preto, SP CEP 14040-903, Brazil. Electronic address: clenimar@usp.br.
authors.forename: Cleni Mara
authors.initials: CM
authors.lastname: Marzocchi-Machado
mesh_terms: D000328:Adult; D000936:Antigen-Antibody Complex / Q000494:pharmacology; D003165:Complement System Proteins / Q000494:pharmacology; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation; D006239:Haplotypes; D006801:Humans; D007519:Isoantigens / Q000235:genetics / Q000276:immunology; D016177:Macrophage-1 Antigen / Q000235:genetics* / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D011110:Polymorphism, Genetic; D061251:Primary Cell Culture; D020239:Receptor Cross-Talk / Q000276:immunology; D017452:Receptors, IgG / Q000235:genetics* / Q000276:immunology; D016897:Respiratory Burst / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction
chemical_list: D000936:Antigen-Antibody Complex; C428200:FCGR3B protein, human; C080568:Fc gamma receptor IIA; D058851:GPI-Linked Proteins; D007519:Isoantigens; D016177:Macrophage-1 Antigen; D017452:Receptors, IgG; C067704:neutrophil-specific antigen NA1, human; D003165:Complement System Proteins
keywords: Complement receptor; Fcγ receptor; Neutrophil; Oxidative burst; Polymorphism