title: Import of ribosomal proteins into yeast mitochondria.
abstract: Mitochondrial ribosomes of baker's yeast contain at least 78 protein subunits. All but one of these proteins are nuclear-encoded, synthesized on cytosolic ribosomes, and imported into the matrix for biogenesis. The import of matrix proteins typically relies on N-terminal mitochondrial targeting sequences that form positively charged amphipathic helices. Interestingly, the N-terminal regions of many ribosomal proteins do not closely match the characteristics of matrix targeting sequences, suggesting that the import processes of these proteins might deviate to some extent from the general import route. So far, the biogenesis of only two ribosomal proteins, Mrpl32 and Mrp10, was studied experimentally and indeed showed surprising differences to the import of other preproteins. In this review article we summarize the current knowledge on the transport of proteins into the mitochondrial matrix, and thereby specifically focus on proteins of the mitochondrial ribosome.
authors.affiliation: a Cell Biology, University of Kaiserslautern, Erwin-Schrödinger-Strasse 13, 67663 Kaiserslautern, Germany.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Woellhaf
authors.forename: Katja G
authors.initials: KG
authors.lastname: Hansen
authors.forename: Christoph
authors.initials: C
authors.lastname: Garth
authors.forename: Johannes M
authors.initials: JM
authors.lastname: Herrmann
mesh_terms: D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C105693:MRP10 protein, S cerevisiae; C505819:MrpL32 protein, S cerevisiae; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins
keywords: mitochondria; mitochondrie; protein translocation; ribosomes; séquences de ciblage; targeting sequences; translocation protéique