title: The epigenome of Trypanosoma brucei: a regulatory interface to an unconventional transcriptional machine.
abstract: The epigenome represents a major regulatory interface to the eukaryotic genome. Nucleosome positions, histone variants, histone modifications and chromatin associated proteins all play a role in the epigenetic regulation of DNA function. Trypanosomes, an ancient branch of the eukaryotic evolutionary lineage, exhibit some highly unusual transcriptional features, including the arrangement of functionally unrelated genes in large, polymerase II transcribed polycistronic transcription units, often exceeding hundreds of kilobases in size. It is generally believed that transcription initiation plays a minor role in regulating the transcript level of genes in trypanosomes, which are mainly regulated post-transcriptionally. Recent advances have revealed that epigenetic mechanisms play an essential role in the transcriptional regulation of Trypanosoma brucei. This suggested that the modulation of gene activity, particularly that of pol I transcribed genes, is, indeed, an important control mechanism, and that the epigenome is critical in regulating gene expression programs that allow the successful migration of this parasite between hosts, as well as the continuous evasion of the immune system in mammalian hosts. A wide range of epigenetic signals, readers, writers and erasers have been identified in trypanosomes, some of which have been mapped to essential genetic functions. Some epigenetic mechanisms have also been observed to be unique to trypanosomes. We review recent advances in our understanding of epigenetic control mechanisms in T. brucei, the causative agent of African sleeping sickness, and highlight the utility of epigenetic targets in the possible development of new therapies for human African trypanosomiasis.
authors.affiliation: Advanced Biomolecular Research Cluster, University of the Free State, PO Box 339, Bloemfontein 9332, South Africa.
authors.forename: Johannes P
authors.initials: JP
authors.lastname: Maree
authors.affiliation: Advanced Biomolecular Research Cluster, University of the Free State, PO Box 339, Bloemfontein 9332, South Africa. Electronic address: patterh@ufs.ac.za.
authors.forename: Hugh-G
authors.initials: HG
authors.lastname: Patterton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057890:Epigenomics; D006801:Humans; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000648:ultrastructure; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics*
chemical_list: D009707:Nucleosomes
keywords: African sleeping sickness; Chromatin; Epigenetics; Gene regulation; Transcription