title: Engineered platform for bioethylene production by a cyanobacterium expressing a chimeric complex of plant enzymes.
abstract: Ethylene is an industrially important compound, but more sustainable production methods are desirable. Since cellulosomes increase the ability of cellulolytic enzymes by physically linking the relevant enzymes via dockerin-cohesin interactions, in this study, we genetically engineered a chimeric cellulosome-like complex of two ethylene-generating enzymes from tomato using cohesin-dockerins from the bacteria Clostridium thermocellum and Acetivibrio cellulolyticus. This complex was transformed into Escherichia coli to analyze kinetic parameters and enzyme complex formation and into the cyanobacterium Synechococcus elongatus PCC 7942, which was then grown with and without 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. Only at minimal protein expression levels (without IPTG), the chimeric complex produced 3.7 times more ethylene in vivo than did uncomplexed enzymes. Thus, cyanobacteria can be used to sustainably generate ethylene, and the synthetic enzyme complex greatly enhanced production efficiency. Artificial synthetic enzyme complexes hold great promise for improving the production efficiency of other industrial compounds.
authors.affiliation: Faculty of Science and Technology, Meijo University , Nagoya, Aichi 468-8502 Japan.
authors.forename: Sadanari
authors.initials: S
authors.lastname: Jindou
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ito
authors.forename: Natsumi
authors.initials: N
authors.lastname: Mito
authors.forename: Keiji
authors.initials: K
authors.lastname: Uematsu
authors.forename: Akifumi
authors.initials: A
authors.lastname: Hosoda
authors.forename: Hiroto
authors.initials: H
authors.lastname: Tamura
mesh_terms: D001419:Bacteria / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism; D044603:Cellulosomes / Q000201:enzymology; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000378:metabolism; D048013:Acetivibrio thermocellus / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism; D005030:Ethylenes / Q000096:biosynthesis* / Q000737:chemistry; D007700:Kinetics; D008190:Lyases / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000201:enzymology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D046940:Synechococcus / Q000235:genetics* / Q000378:metabolism*; D000097722:Cohesins
chemical_list: D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D005030:Ethylenes; D008190:Lyases; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D000097722:Cohesins; C036216:ethylene; C021773:1-aminocyclopropanecarboxylate synthase