title: Single-molecule mechanochemical sensing using DNA origami nanostructures.
abstract: While single-molecule sensing offers the ultimate detection limit, its throughput is often restricted as sensing events are carried out one at a time in most cases. 2D and 3D DNA origami nanostructures are used as expanded single-molecule platforms in a new mechanochemical sensing strategy. As a proof of concept, six sensing probes are incorporated in a 7-tile DNA origami nanoassembly, wherein binding of a target molecule to any of these probes leads to mechanochemical rearrangement of the origami nanostructure, which is monitored in real time by optical tweezers. Using these platforms, 10 pM platelet-derived growth factor (PDGF) are detected within 10 minutes, while demonstrating multiplex sensing of the PDGF and a target DNA in the same solution. By tapping into the rapid development of versatile DNA origami nanostructures, this mechanochemical platform is anticipated to offer a long sought solution for single-molecule sensing with improved throughput.
authors.affiliation: Department of Chemistry & Biochemistry and School of Biomedical Sciences, Kent State University, Kent, OH 44240 (USA).
authors.forename: Deepak
authors.initials: D
authors.lastname: Koirala
authors.forename: Prakash
authors.initials: P
authors.lastname: Shrestha
authors.forename: Tomoko
authors.initials: T
authors.lastname: Emura
authors.forename: Kumi
authors.initials: K
authors.lastname: Hidaka
authors.forename: Shankar
authors.initials: S
authors.lastname: Mandal
authors.forename: Masayuki
authors.initials: M
authors.lastname: Endo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Hanbin
authors.initials: H
authors.lastname: Mao
mesh_terms: D004247:DNA / Q000737:chemistry*; D049329:Nanostructures; D009690:Nucleic Acid Conformation; D052898:Optical Tweezers
chemical_list: D004247:DNA
keywords: DNA nanotechnology; DNA origami nanostructures; mechanochemical sensing; optical tweezers; single-molecule techniques