title: [Effect of Moutan Cortex on AGEs-induced mesangial cell proliferation and basement membrane thickening].
abstract: OBJECTIVE
  To investigate the effect of Moutan Cortex on mesangial proliferation and basement membrane thickening induced by advanced glycation end products (AGEs).
  
  
  METHODS
  The glomerular mesangial cells (MC) injury model was established by inducing by AGEs. The cell were divided into 6 groups: the blank group ( BSA, 200 mg L-1) , the model group (AGEs, 200 mg L-1), the positive control group (AG, 10 mmol L L-1), and drug administration groups, namely the Moutan Cortex-treated high-dose group (2 x 10(-4) g mL(- 1)), the Moutan Cortex-treated medium-dose group (1 x 10(-4) g mL-1 ), and the Moutan Cortex-treated low-dose group (0. 5 x 10(-4) g . mL(-1)). The MTT method was performed to observe the effect of Moutan Cortex on the proliferation of MC. The content of fibronectin (FN) and collagen secretion 1V (Col IV) in cell supernatant were detected by ELISA kits. The western blot analysis was carried out to observe the FN expression. The Real-time PCR analysis was applied to examine the Col IV mRNA expression.
  
  
  RESULTS
  AGEs significantly increased AGEs-induced MC proliferation and FN and Col 1V secretion. The western blot analysis showed that MC could down-regulate the FN expression of MC secretion. According to the results of the real-time PCR assay, MC could down-regulate AGEs-induced MC secretion Col IV mRNA expression.
  
  
  CONCLUSIONS
  MC had a certain protective effect on MC cultured under AGEs conditions. MC could remarkably inhibit the composition and secretion of Col IV and FN in matrix and the basement membrane thickening, and provide an experimental basis for the treatment of diabetic nephropathy.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
authors.forename: Jun-Fei
authors.initials: JF
authors.lastname: Gun
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Jia
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000187:drug effects* / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D024141:Collagen Type IV / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005353:Fibronectins / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects; D017127:Glycation End Products, Advanced / Q000009:adverse effects*; D050527:Mesangial Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D029596:Paeonia
chemical_list: D024141:Collagen Type IV; D004365:Drugs, Chinese Herbal; D005353:Fibronectins; D017127:Glycation End Products, Advanced; C038881:moutan cortex