title: Automated Patch Clamp Analysis of nAChα7 and Na(V)1.7 Channels.
abstract: Automated patch clamp devices are now commonly used for studying ion channels. A useful modification of this approach is the replacement of the glass pipet with a thin planar glass layer with a small hole in the middle. Planar patch clamp devices, such as the three described in this unit, are overtaking glass pipets in popularity because they increase throughput, are easier to use, provide for the acquisition of high-quality and information-rich data, and allow for rapid perfusion and temperature control. Covered in this unit are two challenging targets in drug discovery: voltage-gated sodium subtype 1.7 (Na(V)1.7) and nicotinic acetylcholine α7 receptors (nAChα7R). Provided herein are protocols for recording activation and inactivation kinetics of Na(V)1.7, and activation and allosteric modulation of nAChα7R.
authors.affiliation: Nanion Technologies, Munich, Germany.
authors.forename: Alison
authors.initials: A
authors.lastname: Obergrussberger
authors.forename: Claudia
authors.initials: C
authors.lastname: Haarmann
authors.forename: Ilka
authors.initials: I
authors.lastname: Rinke
authors.forename: Nadine
authors.initials: N
authors.lastname: Becker
authors.forename: David
authors.initials: D
authors.lastname: Guinot
authors.forename: Andrea
authors.initials: A
authors.lastname: Brueggemann
authors.forename: Sonja
authors.initials: S
authors.lastname: Stoelzle-Feix
authors.forename: Michael
authors.initials: M
authors.lastname: George
authors.forename: Niels
authors.initials: N
authors.lastname: Fertig
mesh_terms: D000818:Animals; D057205:Automation, Laboratory; D016466:CHO Cells / Q000502:physiology; D003412:Cricetulus; D057809:HEK293 Cells / Q000502:physiology; D006801:Humans; D062556:NAV1.7 Voltage-Gated Sodium Channel / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods* / Q000592:standards; D064569:alpha7 Nicotinic Acetylcholine Receptor / Q000502:physiology*
chemical_list: D062556:NAV1.7 Voltage-Gated Sodium Channel; D064569:alpha7 Nicotinic Acetylcholine Receptor
keywords: NaV1.7; automated patch clamp; nicotinic acetylcholine receptors