title: Mimicking the extracellular matrix with functionalized, metal-assembled collagen peptide scaffolds.
abstract: Natural and synthetic three-dimensional (3-D) scaffolds that mimic the microenvironment of the extracellular matrix (ECM), with growth factor storage/release and the display of cell adhesion signals, offer numerous advantages for regenerative medicine and in vitro morphogenesis and oncogenesis modeling. Here we report the design of collagen mimetic peptides (CMPs) that assemble into a highly crosslinked 3-D matrix in response to metal ion stimuli, that may be functionalized with His-tagged cargoes, such as green fluorescent protein (GFP-His8) and human epidermal growth factor (hEGF-His6). The bound hEGF-His6 was found to gradually release from the matrix in vitro and induce cell proliferation in the EGF-dependent cell line MCF10A. The additional incorporation of a cell adhesion sequence (RGDS) at the N-terminus of the CMP creates an environment that facilitated the organization of matrix-encapsulated MCF10A cells into spheroid structures, thus mimicking the ECM environment.
authors.affiliation: Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Hernandez-Gordillo
authors.affiliation: Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA. Electronic address: chml@purdue.edu.
authors.forename: Jean
authors.initials: J
authors.lastname: Chmielewski
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry* / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D010455:Peptides; D004815:Epidermal Growth Factor; D003094:Collagen
keywords: Biomimetic material; Cell encapsulation; Collagen; ECM (extracellular matrix); Growth factor; RGD peptide