title: Comparison of three modifications of fused-silica capillaries and untreated capillaries for protein profiling of maize extracts by capillary electrophoresis.
abstract: In this work, capillary electrophoresis was applied to protein profiling of fractionated extracts of maize. A comparative study on the application of uncoated fused-silica capillaries and capillaries modified with hydroxypropylmethylcellulose, ω-iodoalkylammonium salt and a commercially available neutral capillary covalently coated with polyacrylamide is presented. The coating stability, background electrolyte composition, and separation efficiency were investigated. It was found that for zeins separation, the most stable and efficient was the capillary coated with polyacrylamide. Finally, the usefulness of these methods was studied for the differentiation of zein fraction in transgenic and nontransgenic maize. Zeins extracted from maize standards containing 0 and 5% m/m genetic modification were successfully separated, but slight differences were observed in terms of the zein content. Albumin and globulin fractions were analyzed with the use of unmodified fused-silica capillary with borate buffer pH 9 and the capillary coated with polyacrylamide with phosphate buffer pH 3. In the albumin fraction, additional peaks were found in genetically modified samples.
authors.affiliation: Department of Chemistry, Warsaw University, Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Pobozy
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Sentkowska
authors.forename: Anna
authors.initials: A
authors.lastname: Piskor
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D010940:Plant Proteins; D012822:Silicon Dioxide
keywords: Capillary coating; Capillary electrophoresis; Genetic modification; Maize; Protein profiling