title: [LINGO-1 expression of brain tissue in experimental autoimmune encephalomyelitis mouse].
abstract: OBJECTIVE
  To observe the changes of LINGO-1 expression with time after onset in EAE mouse.
  
  
  METHODS
  C57/BL6 mice were completely randomly divided into EAE model group (n = 15) , adjuvant group (n = 15) and control group (n = 15) .LINGO-1 expression of brain tissue was detected on day 1, 7, 14, 21 and 30 after onset by RT-PCR and Western blot.RhoA and p-RhoA expression of brain tissue was analysed by Western blot.
  
  
  RESULTS
  The LINGO-1mRNA levels in EAE model group were markedly higher than control group on day 1, 7and 14 after onset (4.63 ± 0.25, 2.72 ± 0.12, 1.98 ± 0.16, P < 0.01, P < 0.01, P < 0.05).On day 30, Lingo-1 mRNA was close to control group.Expression levels of Lingo-1 protein on day 1, 7, 14, 21, 30 were higher than control group (2.11 ± 0.15, 3.15 ± 0.09, 2.45 ± 0.12, 1.89 ± 0.17, 1.21 ± 0.05, P < 0.05, P < 0.01, P < 0.05, P < 0.05, P < 0.05. The levels of p-RhoA protein increased in EAE and the peak appeared on day 1 and day 7 (P < 0.01) . And there was no difference on RhoA expression among different groups.
  
  
  CONCLUSIONS
  LINGO-1 expression of brain tissue of EAE mouse upregulates and changes with time after onset, which may inhibit myelination by RhoA activation.In clinic, the antagonist of LINGO-1 for MS should be applied as soon as possible.
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Chunjuan
authors.initials: C
authors.lastname: Wang
authors.forename: Shougang
authors.initials: S
authors.lastname: Guo
authors.forename: Chuanqiang
authors.initials: C
authors.lastname: Qu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Peicai
authors.initials: P
authors.lastname: Fu
authors.affiliation: Email: jim1022@126.com.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Tang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000378:metabolism; D020742:rhoA GTP-Binding Protein
chemical_list: C523678:LINGO1 protein, mouse; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C526380:RhoA protein, mouse; D020741:rho GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein