title: An optimized DNA extraction protocol for benthic Didymosphenia geminata.
abstract: Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously described DNA extraction techniques.
authors.affiliation: Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Av. 9 de Julio y Belgrano s/n, (9100) Trelew, Chubut, Argentina.
authors.forename: Noelia Mariel
authors.initials: NM
authors.lastname: Uyua
authors.affiliation: Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Av. 9 de Julio y Belgrano s/n, (9100) Trelew, Chubut, Argentina.
authors.forename: Julieta Marina
authors.initials: JM
authors.lastname: Manrique
authors.affiliation: Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Av. 9 de Julio y Belgrano s/n, (9100) Trelew, Chubut, Argentina. Electronic address: ljones@conicet.gov.ar.
authors.forename: Leandro Roberto
authors.initials: LR
authors.lastname: Jones
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D017377:Diatoms / Q000235:genetics*
chemical_list: D004247:DNA
keywords: Benthic; DNA extraction; Didymosphenia; Invasive; PCR; Stalk