title: Lymphatic fluid for the detection of Mycobacterium avium subsp. paratuberculosis in cows by PCR, compared to fecal sampling and detection of antibodies in blood and milk.
abstract: Johne's disease (JD), caused by Mycobacterium avium subsp. paratuberculosis (MAP), can cause considerable economic losses in affected herds. Early diagnosis of JD is hampered by the chronic nature of the disease with a slow subclincal progression. The aim of the present study was to challenge the hypothesis that lymphatic fluid is of diagnostic value in the early stages of the disease. Lymphatic fluid from 122 animals was collected and tested for MAP by nested PCR for IS900 and compared to the results of testing for MAP in feces (culture), blood and milk (ELISA) in 110 of these samples. MAP was detected by PCR in 27.1% of the lymph samples. Agreement between the tests was poor: 6.9% of the lymph positive cows were also positive in all other tests applied, and 69.0% had negative results in fecal culture, blood and milk ELISA. Resampling of 25 cows after 8 to 12 and 16 to 20 months revealed 20.0% lymph positive animals at the first, 5.5% at the second and 27.8% at the third sampling, respectively. Only one cow showed positive lymph-PCR results at more than one sampling date. Lymph-positive cows had a 7.2 times greater likelihood of being culled within 8 to 12 months after sampling, compared to negative cows, mainly due to other health issues than JD. It can be concluded, that lymphatic fluid might be promising for the detection of early MAP-infection in cows, but further studies to elucidate the potential of this diagnostic approach are needed.
authors.affiliation: Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria. Electronic address: johannes.khol@vetmeduni.ac.at.
authors.forename: Johannes L
authors.initials: JL
authors.lastname: Khol
authors.affiliation: Texas A&M AgriLife Research, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University System, Amarillo, TX, USA.
authors.forename: Pablo J
authors.initials: PJ
authors.lastname: Pinedo
authors.affiliation: Veterinary Medicine Pathology, Department of Infectious Diseases and Pathology, Department of Large Animal Clinical Sciences, University of Florida, Gainesville, FL, USA.
authors.forename: Claus D
authors.initials: CD
authors.lastname: Buergelt
authors.affiliation: Food Animal Reproduction and Medicine Service, Department of Large Animal Clinical Sciences, University of Florida, Gainesville, FL, USA.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Neumann
authors.affiliation: Food Animal Reproduction and Medicine Service, Department of Large Animal Clinical Sciences, University of Florida, Gainesville, FL, USA.
authors.forename: D Owen
authors.initials: DO
authors.lastname: Rae
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D008196:Lymph / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D010283:Paratuberculosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial
keywords: Cattle; Johne's disease; Lymphatic fluid; Mycobacterium avium subsp. paratuberculosis; Polymerase chain reaction; Udder