title: Food Allergen-induced IgE Response Mouse Model Created by Injection of in vitro Differentiated Th2 Cell Culture and Oral Antigen Intake.
abstract: Immunoglobulin (Ig) E is a mediator of food allergic reaction; however, the mechanisms of its production in response to an ingested antigen are not fully understood. For analysis of IgE production, here we propose an IgE response mouse model created by injection of a Th2 cell culture and feeding of an egg white diet. According to this manipulation, total and ovalbumin specific IgE production were elevated in this model. We think our model enables us to analyze IgE induction by Th2 cells in food allergy and can contribute to the development of a treatment for food allergy.
authors.affiliation: Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan ; Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Shibahara
authors.affiliation: Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Haruyo
authors.initials: H
authors.lastname: Nakajima-Adachi
authors.affiliation: Department of Genome Medicine, Pollen Allergy Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Kaminuma
authors.affiliation: Department of Genome Medicine, Pollen Allergy Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan.
authors.forename: Takachika
authors.initials: T
authors.lastname: Hiroi
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, 18-1 Sakuradai, Sagamihara-shi, Kanagawa 252-0392, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
authors.affiliation: Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan ; Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hachimura
keywords: IgE; Th2 cell; food allergy