title: Molecular cloning, characterization and expression of miR-15a-3p and miR-15b-3p in dairy cattle.
abstract: MicroRNA (miRNA) plays an important role in modulating immune responses. We identified miR-15a-3p and miR-15b-3p which were immune related in bovine. Fourteen potential target genes were predicted. Both miR-15a-3p and miR-15b-3p showed significant differential expression patterns in healthy and mastitis infected cattle (P < 0.01). Expression patterns of the two miRNAs provide important information on the relationships between mastitis and miRNA expression.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: PLA Air Force Xi'an Flight Academy, Huxian, Xi'an, Shaanxi 710306, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Song
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
authors.forename: Mengxing
authors.initials: M
authors.lastname: Zhai
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China. Electronic address: liuxiaolin@nwsuaf.edu.cn.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D005260:Female; D020869:Gene Expression Profiling; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000473:pathology; D008414:Mastitis, Bovine / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs
keywords: Dairy cattle; Mastitis; Stem-loop qPCR; miR-15a-3p; miR-15b-3p