title: Enhanced triterpene accumulation in Panax ginseng hairy roots overexpressing mevalonate-5-pyrophosphate decarboxylase and farnesyl pyrophosphate synthase.
abstract: To elucidate the function of mevalonate-5-pyrophosphate decarboxylase (MVD) and farnesyl pyrophosphate synthase (FPS) in triterpene biosynthesis, the genes governing the expression of these enzymes were transformed into Panax ginseng hairy roots. All the transgenic lines showed higher expression levels of PgMVD and PgFPS than that by the wild-type control. Among the hairy root lines transformed with PgMVD, M18 showed the highest level of transcription compared to the control (14.5-fold higher). Transcriptions of F11 and F20 transformed with PgFPS showed 11.1-fold higher level compared with control. In triterpene analysis, M25 of PgMVD produced 4.4-fold higher stigmasterol content (138.95 μg/100 mg, dry weight [DW]) than that by the control; F17 of PgFPS showed the highest total ginsenoside (36.42 mg/g DW) content, which was 2.4-fold higher compared with control. Our results indicate that metabolic engineering in P. ginseng was successfully achieved through Agrobacterium rhizogenes-mediated transformation and that the accumulation of phytosterols and ginsenosides was enhanced by introducing the PgMVD and PgFPS genes into the hairy roots of the plant. Our results suggest that PgMVD and PgFPS play an important role in the triterpene biosynthesis of P. ginseng.
authors.affiliation: Department of Crop Science, Chungnam National University , Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: Yong-Kyoung
authors.initials: YK
authors.lastname: Kim
authors.forename: Yeon Bok
authors.initials: YB
authors.lastname: Kim
authors.forename: Md Romij
authors.initials: MR
authors.lastname: Uddin
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Lee
authors.forename: Soo-Un
authors.initials: SU
authors.lastname: Kim
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D051228:Geranyltranstransferase / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering; D005894:Panax / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified; D058615:Synthetic Biology; D014315:Triterpenes / Q000737:chemistry / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D014315:Triterpenes; D051228:Geranyltranstransferase; D002262:Carboxy-Lyases; C022503:pyrophosphomevalonate decarboxylase
keywords: Panax ginseng; gene expression; overexpression; transgenic hairy root; triterpene