title: Characterization of a polymorphic IGLV gene in pigs (Sus scrofa).
abstract: Swine, unlike other artiodactyls, but similar to humans, utilize both lambda and kappa light chain isotypes almost equally in the generation of their antibody repertoire. The porcine antibody light chain loci have previously been characterized in a single Duroc sow in which was seen extensive allelic variation between light chain genes on homologous chromosomes. However, the extent of variation between individuals is completely unknown. Using deep sequencing of cDNA-derived amplicons from five pigs, we report the identification and characterization of an IGLV gene that is functional and highly expressed in some animals, yet completely absent in others. Our findings provide a possible rationale for the known individual-to-individual variation in antibody responses to vaccination, infectious challenge, and subsequent disease outcome.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN, 55108, USA, john.schwartz@pirbright.ac.uk.
authors.forename: John C
authors.initials: JC
authors.lastname: Schwartz
authors.forename: Michael P
authors.initials: MP
authors.lastname: Murtaugh
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D050439:Genes, Immunoglobulin Light Chain; D059014:High-Throughput Nucleotide Sequencing; D055261:Immunogenetic Phenomena; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017386:Sequence Homology, Amino Acid; D034421:Sus scrofa / Q000235:genetics* / Q000276:immunology*; D050441:VDJ Exons
chemical_list: D018076:DNA, Complementary; D007135:Immunoglobulin Variable Region