title: Purification and identification of a novel antifungal protein secreted by Penicillium citrinum from the Southwest Indian Ocean.
abstract: A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 culture supernatants with ammonium sulfate and ion-exchange chromatography, an antifungal protein (PcPAF) was purified. The N-terminal amino acid sequence analysis showed that PcPAF might be an unknown antifungal protein. PcPAF displayed antifungal activity against Trichoderma viride, Fusarium oxysporum, Paecilomyces variotii, and Alternaria longipes at minimum inhibitory concentrations of 1.52, 6.08, 3.04, and 6.08 µg/disc, respectively. PcPAF possessed high thermostability and had a certain extent of protease and metal ion resistance. The results suggested that PcPAF may represent a novel antifungal protein with potential application in controlling plant pathogenic fungal infection.
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, and Collaborative Innovation Center of Deep Sea Biology, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, P. R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wen
authors.forename: Wenbin
authors.initials: W
authors.lastname: Guo
authors.forename: Xinhua
authors.initials: X
authors.lastname: Chen
mesh_terms: D000528:Alternaria / Q000187:drug effects / Q000254:growth & development; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D055323:Byssochlamys / Q000187:drug effects / Q000254:growth & development; D004355:Drug Stability; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006999:Hypocreales / Q000187:drug effects / Q000254:growth & development; D019156:Indian Ocean; D008826:Microbial Sensitivity Tests; D025282:Mycelium / Q000187:drug effects* / Q000254:growth & development; D010407:Penicillium / Q000302:isolation & purification / Q000378:metabolism*; D055550:Protein Stability; D012623:Seawater / Q000382:microbiology*; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins