title: Thrombus formation induced by laser in a mouse model.
abstract: Animal models are used for the development of techniques and/or models that aid the study of thrombosis pathophysiology. The aim of the present study was to modify the technique ofin vivothrombosis induction to make it more accessible. BALB/c mice were intraperitoneally anesthetized with 0.4 ml 2,2,2-tribromoethanol (266.6 mg/kg) and xylazine (13.3 mg/kg), whilst maintaining stable blood pressure and temperature. Through abdominal surgery, the mesentery was identified and isolated for the visualization of the arteries. A simple epifluorescence magnifier was used to detect the presence of thrombi. The results obtained indicate that using rose bengal at concentrations of 25 and 50 mg/kg and a laser power of 5 mW, thrombus formation occurred. In addition, formation of the thrombus occurred ~30 min following induction and the thrombus had a total area of 4,878.3 μm2, which caused total occlusion of the mesenteric artery. For visualization, platelets were labeled with calcein acetyloxymethyl ester for 1 h, which resulted in improved observation of thrombus formation in real time. Therefore, this technique may be used to performin vivostudies simply and at low cost, and is suitable for use in a variety of studies of thrombosis.
authors.affiliation: Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), University of Talca, Talca, Maule 3460000, Chile.
authors.forename: Pablo
authors.initials: P
authors.lastname: Pérez
authors.affiliation: Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), University of Talca, Talca, Maule 3460000, Chile ; Centro de Estudios en Alimentos Procesados (CEAP), CONICYT-Regional, Talca, Maule R09I2001, Chile.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Alarcón
authors.affiliation: Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), University of Talca, Talca, Maule 3460000, Chile ; Centro de Estudios en Alimentos Procesados (CEAP), CONICYT-Regional, Talca, Maule R09I2001, Chile.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Fuentes
authors.affiliation: Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), University of Talca, Talca, Maule 3460000, Chile ; Centro de Estudios en Alimentos Procesados (CEAP), CONICYT-Regional, Talca, Maule R09I2001, Chile.
authors.forename: Iván
authors.initials: I
authors.lastname: Palomo
keywords: platelet; rose bengal; thrombosis formation