title: Characterization, tissue distribution and regulation of neuropeptideY in Schizothorax prenanti.
abstract: In this study, the full-length neuropeptide Y (npy) complementary (c)DNA was cloned in ya fish Schizothorax prenanti. npy cDNA was composed of 789 nucleotides with a 288 nucleotide open reading frame encoding a protein of 96 amino acids. The deduced amino acid sequences contained a 28 amino acids signal peptide followed by a 36 amino acids mature neuropeptide Y (NPY). The npy mRNA was expressed mainly in the brain and eye as detected by real-time quantitative polymerase chain reaction RT-PCR (rt-qPCR). The S. prenanti NPY was detectable from blastulation to hatch, suggesting that npy might be involved in the late embryonic development of S. prenanti. An experiment was conducted to determine the expression profile of npy during feeding of a single meal and during long-term fasting. The expression level of npy in fed fish was significantly decreased at 0.5, 1.5, 3 and 9 h post-feeding (hpf) than in fasting fish. Fasting for 14 days induced an increase in npy messenger (m)RNA expression in the brain. Overall, the results suggest that NPY is a conserved peptide that might be involved in the regulation of feeding and other physiological function in S. prenanti.
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Yaan, China.
authors.forename: R
authors.initials: R
authors.lastname: Wei
authors.forename: C
authors.initials: C
authors.lastname: Zhou
authors.forename: D
authors.initials: D
authors.lastname: Yuan
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Lin
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: Z
authors.initials: Z
authors.lastname: Xin
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: D
authors.initials: D
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: Z
authors.initials: Z
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics*; D004435:Eating / Q000502:physiology; D005123:Eye / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000235:genetics*; D016415:Sequence Alignment; D059467:Transcriptome
chemical_list: D029941:Fish Proteins; D009478:Neuropeptide Y
keywords: appetite regulation; cloning; expression pattern; structure