title: Morphofunctional characterization of decellularized vena cava as tissue engineering scaffolds.
abstract: Clinical experience for peripheral arterial disease treatment shows poor results when synthetic grafts are used to approach infrapopliteal arterial segments. However, tissue engineering may be an option to yield surrogate biocompatible neovessels. Thus, biological decellularized scaffolds could provide natural tissue architecture to use in tissue engineering, when the absence of ideal autologous veins reduces surgical options. The goal of this study was to evaluate different chemical induced decellularization protocols of the inferior vena cava of rabbits. They were decellularized with Triton X100 (TX100), sodium dodecyl sulfate (SDS) or sodium deoxycholate (DS). Afterwards, we assessed the remaining extracellular matrix (ECM) integrity, residual toxicity and the biomechanical resistance of the scaffolds. Our results showed that TX100 was not effective to remove the cells, while protocols using SDS 1% for 2h and DS 2% for 1h, efficiently removed the cells and were better characterized. These scaffolds preserved the original organization of ECM. In addition, the residual toxicity assessment did not reveal statistically significant changes while decellularized scaffolds retained the equivalent biomechanical properties when compared with the control. Our results concluded that protocols using SDS and DS were effective at obtaining decellularized scaffolds, which may be useful for blood vessel tissue engineering.
authors.affiliation: Department of Surgery and Orthopedics, Botucatu Medical School, São Paulo State University (UNESP), Vascular Laboratory, Avenue Prof. Montenegro, S/N. Rubião Júnior, Botucatu 18618-970, SP, Brazil; Blood Transfusion Center, Cell Engineering Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil. Electronic address: matheus.fameca@ig.com.br.
authors.forename: Matheus
authors.initials: M
authors.lastname: Bertanha
authors.affiliation: Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, SP, Brazil.
authors.forename: Andrei
authors.initials: A
authors.lastname: Moroz
authors.affiliation: Department of Surgery and Orthopedics, Botucatu Medical School, São Paulo State University (UNESP), Vascular Laboratory, Avenue Prof. Montenegro, S/N. Rubião Júnior, Botucatu 18618-970, SP, Brazil.
authors.forename: Rodrigo G
authors.initials: RG
authors.lastname: Jaldin
authors.affiliation: Blood Transfusion Center, Cell Engineering Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.
authors.forename: Regina A M
authors.initials: RA
authors.lastname: Silva
authors.affiliation: Department of Morphology, Extracellular Matrix Laboratory, Botucatu Biosciences Institute, São Paulo State University (UNESP), Botucatu, SP, Brazil.
authors.forename: Jaqueline C
authors.initials: JC
authors.lastname: Rinaldi
authors.affiliation: Blood Transfusion Center, Flow Cytometry Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.
authors.forename: Márjorie A
authors.initials: MA
authors.lastname: Golim
authors.affiliation: Department of Morphology, Extracellular Matrix Laboratory, Botucatu Biosciences Institute, São Paulo State University (UNESP), Botucatu, SP, Brazil.
authors.forename: Sérgio L
authors.initials: SL
authors.lastname: Felisbino
authors.affiliation: Department of Pathology, Immunohistochemistry Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.
authors.forename: Maria A C
authors.initials: MA
authors.lastname: Domingues
authors.affiliation: Department of Surgery and Orthopedics, Botucatu Medical School, São Paulo State University (UNESP), Vascular Laboratory, Avenue Prof. Montenegro, S/N. Rubião Júnior, Botucatu 18618-970, SP, Brazil.
authors.forename: Marcone L
authors.initials: ML
authors.lastname: Sobreira
authors.affiliation: Department of Surgery and Orthopedics, Botucatu Medical School, São Paulo State University (UNESP), Vascular Laboratory, Avenue Prof. Montenegro, S/N. Rubião Júnior, Botucatu 18618-970, SP, Brazil.
authors.forename: Patricia P
authors.initials: PP
authors.lastname: Reis
authors.affiliation: Blood Transfusion Center, Cell Engineering Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil; Department of Urology, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.
authors.forename: Elenice
authors.initials: E
authors.lastname: Deffune
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001696:Biomechanical Phenomena; D002454:Cell Differentiation; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry; D005260:Female; D007124:Immunoenzyme Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011817:Rabbits; D013501:Surface-Active Agents / Q000494:pharmacology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D016378:Tissue Transplantation; D014682:Vena Cava, Inferior / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*
chemical_list: D013501:Surface-Active Agents
keywords: Biomechanics; Blood vessels; Extracellular matrix; Peripheral arterial disease; Tissue engineering