title: Advances in fluorescence labeling strategies for dynamic cellular imaging.
abstract: Synergistic advances in optical physics, probe design, molecular biology, labeling techniques and computational analysis have propelled fluorescence imaging into new realms of spatiotemporal resolution and sensitivity. This review aims to discuss advances in fluorescent probes and live-cell labeling strategies, two areas that remain pivotal for future advances in imaging technology. Fluorescent protein- and bio-orthogonal-based methods for protein and RNA imaging are discussed as well as emerging bioengineering techniques that enable their expression at specific genomic loci (for example, CRISPR and TALENs). Important attributes that contribute to the success of each technique are emphasized, providing a guideline for future advances in dynamic live-cell imaging.
authors.affiliation: 1] Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado, USA. [2] BioFrontiers Institute, University of Colorado, Boulder, Colorado, USA.
authors.forename: Kevin M
authors.identifier: 0000000308392320
authors.initials: KM
authors.lastname: Dean
authors.affiliation: 1] Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado, USA. [2] BioFrontiers Institute, University of Colorado, Boulder, Colorado, USA.
authors.forename: Amy E
authors.initials: AE
authors.lastname: Palmer
mesh_terms: D000818:Animals; D057005:Bioengineering / Q000295:instrumentation / Q000379:methods; D005057:Eukaryotic Cells / Q000378:metabolism / Q000648:ultrastructure; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008027:Light; D008164:Luminescent Proteins / Q000737:chemistry*; D008958:Models, Molecular; D057054:Molecular Imaging / Q000379:methods*; D012313:RNA / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D013816:Thermodynamics; D059008:Time-Lapse Imaging
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D012313:RNA