title: The effects of CoCl2 on HIF-1α protein under experimental conditions of autoprogressive hypoxia using mouse models.
abstract: It is well known that cobalt chloride (CoCl2) can enhance the stability of hypoxia-inducible factor (HIF)-1α. The aim of this study is to detect the effect of CoCl2 on the hypoxia tolerance of mice which were repeatedly exposed to autoprogressive hypoxia. Balb/c mice were randomly divided into groups of chemical pretreatment and normal saline (NS), respectively injected with CoCl2 and NS 3 h before exposure to hypoxia for 0 run (H0), 1 run (H1), and 4 runs (H4). Western Blot, electrophoretic mobility shift assay (EMSA), extracellular recordings population spikes in area cornus ammonis I (CA 1) of mouse hippocampal slices and real-time were used in this study. Our results demonstrated that the tolerance of mice to hypoxia, the changes of HIF-1α protein level and HIF-1 DNA binding activity in mice hippocampus, the mRNA level of erythropoietin (EPO) and vascular endothelial growth factor (VEGF), and the disappearance time of population spikes of hippocampal slices were substantially different between the control group and the CoCl2 group. Over-induction of HIF-1α by pretreatment with CoCl2 before hypoxia did not increase the hypoxia tolerance.
authors.affiliation: Department of Neurology, Affiliated Hospital of Tai Shan Medical University, Taishan 271000, China. bbnnbn@163.com.
authors.forename: Yan-Bo
authors.initials: YB
authors.lastname: Zhang
authors.affiliation: Department of Intensive Care Unit , 2nd Affiliated Hospital of Baotou Medical College, Baotou 014030, China. zhangjianjunnmg@163.com.
authors.forename: Xiulian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Medicine, University of Arizona, Tucson, AZ 85721, USA. eemeister@yahoo.com.
authors.forename: Edward A
authors.initials: EA
authors.lastname: Meister
authors.affiliation: Biomedicine Research Center and Basic Medical College, Baotou Medical College, Baotou 014060, China. keruigong@gmail.com.
authors.forename: Ke-Rui
authors.initials: KR
authors.lastname: Gong
authors.affiliation: Biomedicine Research Center and Basic Medical College, Baotou Medical College, Baotou 014060, China. yanshaochunby@163.com.
authors.forename: Shao-Chun
authors.initials: SC
authors.lastname: Yan
authors.affiliation: Institute for Hypoxia Medicine, Xuanwu Hospital of Capital Medical University, Beijing 10054, China. gwlu32@163.com.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Lu
authors.affiliation: Institute for Hypoxia Medicine, Xuanwu Hospital of Capital Medical University, Beijing 10054, China. robertjixm@hotmail.com.
authors.forename: Xun-Ming
authors.initials: XM
authors.lastname: Ji
authors.affiliation: Biomedicine Research Center and Basic Medical College, Baotou Medical College, Baotou 014060, China. shao.guo.china@gmail.com.
authors.forename: Guo
authors.initials: G
authors.lastname: Shao
mesh_terms: D000818:Animals; D003035:Cobalt / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D004921:Erythropoietin / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D000860:Hypoxia; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D004921:Erythropoietin; D003035:Cobalt; D004247:DNA; C018021:cobaltous chloride