title: Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2.
abstract: The aim of this study was to compare and correlate antibody titres against porcine circovirus type 2 (PCV2) in porcine sera (n = 1270) obtained by immunoperoxidase monolayer assay (IPMA) with the results of three commercial ELISAs (designated E1, E2 and E3). The correlation between IPMA and ELISA results was excellent (r(2) ≥ 0.90). Compared to IPMA, E2 had the highest sensitivity (93.0%), followed by E3 (90.1%) and E1 (85.0%); the specificity was 100% for all tests. All three commercial ELISAs had predictive values similar to those of IPMA and could be used to monitor antibody responses against PCV2 infection and/or vaccination.
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain; Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain.
authors.forename: E
authors.initials: E
authors.lastname: Pileri
authors.affiliation: Pirbright Institute, Ash Road, Woking GU24 0NF, UK.
authors.forename: M
authors.initials: M
authors.lastname: Cortey
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain.
authors.forename: F
authors.initials: F
authors.lastname: Rodríguez
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain.
authors.forename: M
authors.initials: M
authors.lastname: Sibila
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain; Universitat de Lleida, Lleida, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Fraile
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain; Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain. Electronic address: joaquim.segales@cresa.uab.cat.
authors.forename: J
authors.initials: J
authors.lastname: Segalés
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D018173:Circoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology
chemical_list: D000914:Antibodies, Viral
keywords: ELISA; Immunoperoxidase monolayer assay; Porcine circovirus type 2; Serology