title: Fluorescent chitosan functionalized magnetic polymeric nanoparticles: Cytotoxicity and in vitro evaluation of cellular uptake.
abstract: Nanoparticles possessing magnetic and fluorescent properties were fabricated by the covalent attachment of fluorescein isothiocyanate onto magnetic polymeric nanoparticles functionalized by chitosan. The synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate were successfully used for labeling the living organ and blood-related cancer cells, i.e., HeLa, Hep G2, and K562 cells. The cytotoxicity test of nanoparticles at various incubation times indicated the high cell viability (>90%) without morphological change. The confocal microscopy revealed that they could pass through cell membrane within 2 h for K562 cells and 3 h for HeLa and Hep G2 cells and then confine inside cytoplasm of all types of tested cells for at least 24 h. Therefore, the synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate would potentially be used as cell tracking in theranostic applications.
authors.affiliation: Department of Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Chariya
authors.initials: C
authors.lastname: Kaewsaneha
authors.affiliation: Department of Clinical Microbiology and Applied Technology, Center for Innovation Development and Technology Transfer, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
authors.forename: Kulachart
authors.initials: K
authors.lastname: Jangpatarapongsa
authors.affiliation: Department of Clinical Microbiology and Applied Technology, Center for Innovation Development and Technology Transfer, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
authors.forename: Tienrat
authors.initials: T
authors.lastname: Tangchaikeeree
authors.affiliation: National Nanotechnology Center (NANOTEC), PathumThani, Thailand.
authors.forename: Duangporn
authors.initials: D
authors.lastname: Polpanich
authors.affiliation: Department of Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand pramuan.tan@mahidol.ac.th.
authors.forename: Pramuan
authors.initials: P
authors.lastname: Tangboriboonrat
mesh_terms: D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D020014:K562 Cells; D008280:Magnetics; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011108:Polymers; D048271:Chitosan; D016650:Fluorescein-5-isothiocyanate
keywords: Magnetic; cell labeling; chitosan; fluorescence; nanoparticle