title: Tumor microenvironment and RIG-I signaling molecules in Epstein Barr virus-positive and -negative classical Hodgkin lymphoma of the elderly.
abstract: Classical Hodgkin lymphoma (CHL) is a B-cell neoplasm characterized by Hodgkin and Reed-Sternberg (HRS) cells. Its prevalence exhibits a bimodal pattern of peaking in young adults and the elderly. There is an association with Epstein-Barr virus (EBV) infection in about 50% of cases of CHL of the elderly, and the outcome of these patients is unfavorable. It is not well known how the latent infection of EBV is involved in the pathophysiology of CHL of the elderly. To address this issue, we examined the tumor microenvironment (TME) and the expression of molecules related to EBV infection in HRS cells in 10 EBV-positive CHL and 7 EBV-negative CHL patients older than 50 years. In EBV-positive CHL, we found an increased population of FOXP3(+) cells, while that of granzyme B(+) cells was reduced, compared with those in EBV-negative CHL. The expression of inhibitory chemokine CCL20 was increased in EBV-positive HRS cells compared with that in EBV-negative HRS cells. In addition, despite increased expression of a pattern recognition receptor, RIG-I, in intracellular innate immunity, there was no evidence of interferon regulatory factor 3 activation or interferon-ß induction in EBV-positive HRS cells in CHL of the elderly. The disease recurred frequently (50%) in EBV-positive CHL. The current study thus suggests the possibility that the latent infection of EBV alters the expression of chemokines and the innate immunity response in HRS cells and modulates TME to an immunosuppressive state, which may account for the unfavorable disease course in CHL of the elderly.
authors.affiliation: Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine.
authors.forename: Tsugumi
authors.initials: T
authors.lastname: Satoh
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Wada
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Yajima
authors.forename: Tadaatsu
authors.initials: T
authors.lastname: Imaizumi
authors.forename: Soroku
authors.initials: S
authors.lastname: Yagihashi
mesh_terms: D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D054418:Chemokine CCL20 / Q000235:genetics / Q000378:metabolism; D020031:Epstein-Barr Virus Infections / Q000150:complications*; D005260:Female; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000235:genetics; D006689:Hodgkin Disease / Q000175:diagnosis / Q000209:etiology* / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism*; D016539:Reed-Sternberg Cells / Q000473:pathology; D015398:Signal Transduction; D059016:Tumor Microenvironment; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D054418:Chemokine CCL20; C037662:EBV-associated membrane antigen, Epstein-Barr virus; C437968:PLAAT4 protein, human; D018168:Receptors, Retinoic Acid; D014763:Viral Matrix Proteins