title: PCR and ELISA (VIDAS ECO O157(®)) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO.
abstract: Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157(®), bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA, and 23,33% with PCR. The pathogen was not detected in samples from industries under Federal Inspection.
authors.affiliation: Embrapa Arroz e Feijão Santo Antônio de Goiás GoiásGO Brazil.
authors.forename: Rosangela Nunes
authors.initials: RN
authors.lastname: Carvalho
authors.affiliation: Centro de Pesquisa em Alimentos Escola de Veterinária e Zootecnia Universidade Federal de Goiás GoiâniaGO Brazil.
authors.forename: Antonio Nonato
authors.initials: AN
authors.lastname: de Oliveira
authors.affiliation: Centro de Pesquisa em Alimentos Escola de Veterinária e Zootecnia Universidade Federal de Goiás GoiâniaGO Brazil.
authors.forename: Albenones José
authors.initials: AJ
authors.lastname: de Mesquita
authors.affiliation: Centro de Pesquisa em Alimentos Escola de Veterinária e Zootecnia Universidade Federal de Goiás GoiâniaGO Brazil.
authors.forename: Cíntia Silva
authors.initials: CS
authors.lastname: Minafra e Rezende
authors.affiliation: Centro de Pesquisa em Alimentos Escola de Veterinária e Zootecnia Universidade Federal de Goiás GoiâniaGO Brazil.
authors.forename: Adriano Queiroz
authors.initials: AQ
authors.lastname: de Mesquita
authors.affiliation: Centro de Pesquisa em Alimentos Escola de Veterinária e Zootecnia Universidade Federal de Goiás GoiâniaGO Brazil.
authors.forename: Rolando Alfredo Mazzoni
authors.initials: RA
authors.lastname: Romero
mesh_terms: D002611:Cheese / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019453:Escherichia coli O157 / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence
keywords: Minas Frescal cheese; PCR; STEC/VTEC; VIDAS ECO O157®