title: A highly tunable system for the simultaneous expression of multiple enzymes in Saccharomyces cerevisiae.
abstract: Control of the expression levels of multiple enzymes in transgenic yeasts is essential for the effective production of complex molecules through fermentation. Here, we propose a tunable strategy for the control of expression levels based on the design of terminator regions and other gene-expression control elements in Saccharomyces cerevisiae. Our genome-integrated system, which is capable of producing high expression levels over a wide dynamic range, will broadly enable metabolic engineering and synthetic biology. We demonstrated that the activities of multiple cellulases and the production of ethanol were doubled in a transgenic yeast constructed with our system compared with those achieved with a standard expression system.
authors.affiliation: Matsuyama Research Group and ‡Biotechnology Laboratory, Toyota Central Research and Development Laboratories, Inc. , 41-1 Yokomichi, Nagakute, Aichi 480-1192, Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Mamoru
authors.initials: M
authors.lastname: Yamanishi
authors.forename: Akinori
authors.initials: A
authors.lastname: Ikeuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuyama
mesh_terms: D044602:Cellulases / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D015870:Gene Expression; D005813:Genes, Synthetic; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D058615:Synthetic Biology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D000431:Ethanol; D002482:Cellulose; D044602:Cellulases