title: New plant-parasitic nematode from the mostly mycophagous genus Bursaphelenchus discovered inside figs in Japan.
abstract: A new nematode species, Bursaphelenchus sycophilus n. sp. is described. The species was found in syconia of a fig species, Ficus variegata during a field survey of fig-associated nematodes in Japan. Because it has a well-developed stylet and pharyngeal glands, the species is considered an obligate plant parasite, and is easily distinguished from all other fungal-feeding species in the genus based upon these characters. Although B. sycophilus n. sp. shares an important typological character, male spicule possessing a strongly recurved condylus, with the "B. eremus group" and the "B. leoni group" of the genus, it was inferred to be monophyletic with the "B. fungivorus group". The uniquely shaped stylet and well-developed pharyngeal glands is reminiscent of the fig-floret parasitic but paraphyletic assemblage of "Schistonchus". Thus, these morphological characters appear to be an extreme example of convergent evolution in the nematode family, Aphelenchoididae, inside figs. Other characters shared by the new species and its close relatives, i.e., lack of ventral P1 male genital papilla, female vulval flap, and papilla-shaped P4 genital papillae in males, corroborate the molecular phylogenetic inference. The unique biological character of obligate plant parasitism and highly derived appearance of the ingestive organs of Bursaphelenchus sycophilus n. sp. expands our knowledge of the potential morphological, physiological and developmental plasticity of the genus Bursaphelenchus.
authors.affiliation: Department of Forest Microbiology, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Kanzaki
authors.affiliation: Division of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, Miyazaki, Japan.
authors.forename: Ryusei
authors.initials: R
authors.lastname: Tanaka
authors.affiliation: Fort Lauderdale Research and Education Center, University of Florida/IFAS, Davie, Florida, United States of America.
authors.forename: Robin M
authors.initials: RM
authors.lastname: Giblin-Davis
authors.affiliation: Centre for Evolutionary Biology and Biodiversity, School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Glen Osmond, South Australia, Australia.
authors.forename: Kerrie A
authors.initials: KA
authors.lastname: Davies
mesh_terms: D000818:Animals; D005260:Female; D030681:Ficus / Q000469:parasitology*; D017238:Genes, Helminth; D007564:Japan; D008297:Male; D058889:Molecular Typing; D009348:Nematoda / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics
chemical_list: D012335:RNA, Ribosomal