title: Association ofXRCC1andXRCC3gene haplotypes with the development of radiation-induced fibrosis in patients with nasopharyngeal carcinoma.
abstract: Radiation-induced fibrosis is one of the late complications of radiotherapy (RT) for nasopharyngeal carcinoma (NPC). The aim of this study was to investigate the association between X-ray repair cross-complementing protein 1 and 3 (XRCC1andXRCC3, respectively) gene haplotypes and radiation-induced fibrosis in NPC patients. Genomic DNA was extracted from blood samples of 120 NPC patients previously treated with RT. In total, 12 tag single-nucleotide polymorphisms (SNPs) were selected from theXRCC1andXRCC3genes and were genotyped using restriction fragment length polymorphism analysis or unlabeled probe melting analysis. Single-marker and haplotype analyses were performed using multivariate logistic regression analysis. The functional variant rs861539 ofXRCC3may be associated with radiation-induced fibrosis [asymptotic P-value (Pasym)<0.05]. No significant association was observed between radiation-induced fibrosis and any of the tag SNPs ofXRCC1andXRCC3in either single-marker or haplotype analysis after 10,000 permutations [empirical P-value (Pemp)>0.05]. Our preliminary results indicated that the rs861539 variant ofXRCC3may be associated with an increased risk of radiation-induced fibrosis; however, a large-scale study is required to confirm this result.
authors.affiliation: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, SAR, P.R. China.
authors.forename: Isabella Wai Yin
authors.initials: IW
authors.lastname: Cheuk
authors.affiliation: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, SAR, P.R. China.
authors.forename: Shea Ping
authors.initials: SP
authors.lastname: Yip
authors.affiliation: Department of Clinical Oncology, Queen Mary Hospital, The University of Hong Kong, Pok Fu Lam, Hong Kong, SAR, P.R. China.
authors.forename: Dora Lai Wan
authors.initials: DL
authors.lastname: Kwong
authors.affiliation: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, SAR, P.R. China.
authors.forename: Vincent Wing Cheung
authors.initials: VW
authors.lastname: Wu
keywords: X-ray repair cross-complementing protein 1; X-ray repair cross-complementing protein 3; chronic fibrosis; nasopharyngeal carcinoma; radiation toxicities