title: Catalase activity in blood fractions of patients with sporadic ALS.
abstract: BACKGROUND
  Oxidative stress may be a key element in pathogenesis of sporadic amyotrophic lateral sclerosis (sALS). Several studies proved that markers of peroxidation of lipids, proteins or nucleic acids are increased in postmortem tissue of sALS patients. However, much less is known about enzymatic antioxidant defense mechanism in sALS.
  
  
  OBJECTIVE
  The aim of the study was to assess catalase (CAT) activity that is implicated in the defense against oxidative stress, in three blood fractions, i.e. erythrocytes, plasma and serum of sALS patients and healthy controls.
  
  
  METHODS
  Altogether 46 sALS patients and 54 controls were enrolled in the study. CAT activity was estimated using a commercially available assay kit.
  
  
  RESULTS
  CAT activity in erythrocytes of sALS patients was significantly decreased compared to neurologically healthy controls (p=0.04), whereas CAT activity in plasma and serum was similar in both studied groups.
  
  
  CONCLUSIONS
  CAT activity in erythrocytes, in contrast to other blood fractions is reduced in sALS cases as compared to controls, which may indirectly indicate that antioxidant defense system in erythrocytes is involved in pathogenesis of sALS.
authors.affiliation: Jagiellonian University Medical College, Department of Neurology, Kraków, Poland. Electronic address: agolenia@gmail.com.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Golenia
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland. Electronic address: leskiew@if-pan.krakow.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Leśkiewicz
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland. Electronic address: regulska@if-pan.krakow.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Regulska
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland. Electronic address: budzisz@if-pan.krakow.pl.
authors.forename: Bogusława
authors.initials: B
authors.lastname: Budziszewska
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland. Electronic address: szczesny@if-pan.krakow.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Szczęsny
authors.affiliation: Jagiellonian University Medical College, Department of Neurology, Kraków, Poland. Electronic address: jeremiasz.jagiella@gmail.com.
authors.forename: Jeremiasz
authors.initials: J
authors.lastname: Jagiełła
authors.affiliation: Jagiellonian University Medical College, Department of Neurology, Kraków, Poland. Electronic address: marcin.a.wnuk@gmail.com.
authors.forename: Marcin
authors.initials: M
authors.lastname: Wnuk
authors.affiliation: Jagiellonian University Medical College, Department of Neurology, Kraków, Poland. Electronic address: mostroff@interia.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Ostrowska
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland. Electronic address: lason@if-pan.krakow.pl.
authors.forename: Władysław
authors.initials: W
authors.lastname: Lasoń
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland. Electronic address: basta@if-pan.krakow.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Basta-Kaim
authors.affiliation: Jagiellonian University Medical College, Department of Neurology, Kraków, Poland. Electronic address: slowik@neuro.cm-uj.krakow.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Słowik
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000097:blood* / Q000201:enzymology; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D015227:Lipid Peroxidation; D018384:Oxidative Stress
chemical_list: D000975:Antioxidants; D002374:Catalase
keywords: Catalase activity; Motor neuron disease; Oxidative stress; Sporadic amyotrophic lateral sclerosis