title: Sperm involved in recurrent partial hydatidiform moles cannot induce the normal pattern of calcium oscillations.
abstract: OBJECTIVE
  To assess the Ca2+-releasing ability of sperm involved in partial hydatidiform moles.
  
  
  METHODS
  Analysis of the activating and Ca2+-releasing ability of human sperm.
  
  
  METHODS
  University hospital research laboratory.
  
  
  METHODS
  Patients undergoing intracytoplasmic sperm injection (ICSI) treatment.
  
  
  METHODS
  Microinjection of mouse and human oocytes with sperm.
  
  
  METHODS
  Measurement of the fertilizing and Ca2+-releasing ability of human sperm.
  
  
  RESULTS
  The mouse oocyte Ca2+ analysis showed that only 19.0% (4/21) of the mouse oocytes injected with sperm involved in molar pregnancies exhibited a normal pattern of Ca2+ oscillations versus 63.2% (36/57) of those injected with control sperm. Further, 83.3% (15/18) of donated in vitro-matured human oocytes injected with deficient sperm did not exhibit any Ca2+ release, while 76.9% (10/13) failed to show normal pronuclear development. Yet the sperm oocyte activation factor phospholipase C zeta (PLCζ) was present in the majority (96.6%, n=113) of the analyzed sperm at a normal expression level. Eventually, fertilization failure was overcome with assisted oocyte activation in subsequent therapeutic ICSI cycles, which led to normal deliveries.
  
  
  CONCLUSIONS
  Sperm that previously provoked recurrent partial hydatidiform mole pregnancies due to dispermic fertilization is not able to activate human oocytes or trigger the normal pattern of Ca2+ oscillations in mouse and human oocytes in vitro.
authors.affiliation: Ghent Fertility and Stem Cell Team, Ghent University Hospital, Ghent, Belgium.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Nikiforaki
authors.affiliation: Ghent Fertility and Stem Cell Team, Ghent University Hospital, Ghent, Belgium.
authors.forename: Frauke
authors.initials: F
authors.lastname: Vanden Meerschaut
authors.affiliation: Ghent Fertility and Stem Cell Team, Ghent University Hospital, Ghent, Belgium.
authors.forename: Stefanie
authors.initials: S
authors.lastname: De Gheselle
authors.affiliation: Ghent Fertility and Stem Cell Team, Ghent University Hospital, Ghent, Belgium.
authors.forename: Chen
authors.initials: C
authors.lastname: Qian
authors.affiliation: Ghent Fertility and Stem Cell Team, Ghent University Hospital, Ghent, Belgium.
authors.forename: Etienne
authors.initials: E
authors.lastname: Van den Abbeel
authors.affiliation: Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, Antwerp, Belgium; Cell Systems and Imaging Research Group, Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
authors.forename: Winnok Harald
authors.initials: WH
authors.lastname: De Vos
authors.affiliation: Ghent Fertility and Stem Cell Team, Ghent University Hospital, Ghent, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Deroo
authors.affiliation: Ghent Fertility and Stem Cell Team, Ghent University Hospital, Ghent, Belgium.
authors.forename: Petra
authors.initials: P
authors.lastname: De Sutter
authors.affiliation: Ghent Fertility and Stem Cell Team, Ghent University Hospital, Ghent, Belgium. Electronic address: Bjorn.Heindryckx@UGent.be.
authors.forename: Björn
authors.initials: B
authors.lastname: Heindryckx
mesh_terms: D000328:Adult; D000818:Animals; D020013:Calcium Signaling; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D007246:Infertility / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D008297:Male; D051379:Mice; D018587:Oocyte Donation; D009865:Oocytes / Q000378:metabolism* / Q000473:pathology; D054801:Phosphoinositide Phospholipase C / Q000378:metabolism; D011247:Pregnancy; D012008:Recurrence; D020554:Sperm Injections, Intracytoplasmic; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology; D016896:Treatment Outcome; D057885:Triploidy; D014594:Uterine Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: C499235:PLCZ1 protein, human; D054801:Phosphoinositide Phospholipase C; C499234:Plcz1 protein, mouse
keywords: Hydatidiform mole; calcium; dispermy; triploidy