title: [Effect of precursor on growth and accumulation of alkaloids of Lycoris radiata suspension cells].
abstract: In order to investigate the effects of phenylalanine, tyrosine and tyramine on the growth of Lycoris radiata suspension cells and the accumulation of alkaloids, the growth quantity of the cells as well as the content of alkaloids in cells were determined, which were treated with above three kinds of precursors alone and phenylalanine combined with tyrosine respectively. The results indicate that the addition of phenylalanine alone and addition of phenylalanine on the basis of tyrosine at high concentration (200 micromol/L) had no significant effect on the growth of Lycoris radiata suspension cells and the content of alkaloids in cells; whereas tyrosine and tyramine promoted the growth of the cells and alkaloids accumulation. Treated with tyrosine at high concentration (200 micromol/L), the content of alkaloids of the cells was 2.56-fold higher than that of the control group, the amounts of lycoramine (3.77 mg/g) and galanthamine (4.46 mg/g) were 6.61-fold and 6.97-fold higher than that of the control group, respectively. When treated with tyramine (200 micromol/L), the amount of alkaloids in Lycoris radiata suspension cells was 2.63-fold higher than that of the control group, and the amounts of lycoramine (4.45 mg/g) and galanthamine (5.14 mg/g) were 9.08-fold and 9.18-fold higher than that of the control group, respectively. The above results demonstrate that adding tyrosine and tyramine in the media significantly promoted the growth of the Lycoris radiata suspension cells and alkaloids accumulation in the cells.
authors.forename: Yuqiong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Jianhui
authors.initials: J
authors.lastname: Zhou
authors.forename: Na
authors.initials: N
authors.lastname: Chen
authors.forename: Meifang
authors.initials: M
authors.lastname: Wang
authors.forename: Zhaorong
authors.initials: Z
authors.lastname: Dong
authors.forename: Cuiyun
authors.initials: C
authors.lastname: Gao
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Zhong
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000737:chemistry*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D005702:Galantamine / Q000737:chemistry; D031426:Lycoris / Q000737:chemistry / Q000254:growth & development*; D010649:Phenylalanine / Q000737:chemistry; D059828:Plant Cells / Q000737:chemistry*; D010936:Plant Extracts
chemical_list: D047151:Amaryllidaceae Alkaloids; D003470:Culture Media; D010936:Plant Extracts; D005702:Galantamine; D010649:Phenylalanine; C500482:lycoramine