title: Development of species-specific quantitative real-time PCR primers for detecting anginosus group streptococci based on the rpoB.
abstract: In this study, we introduced species-specific quantitative real-time PCR (qPCR) primers designed based on a DNA-dependent RNA polymerase beta-subunit gene for detecting anginosus group streptococci (AGS), Streptococcus anginosus, S. constellatus, and S. intermedius. The specificity of the qPCR primers was confirmed by conventional PCR with the genomic DNAs of 76 strains regarding 44 bacterial species including the type strain for the target species. The standard curves revealed the lower detection limits of these species-specific qPCR primers was 40 fg at below a cycle threshold (CT) value of 35. These results suggest that AGS species-specific qPCR primers are suitable for applications in epidemiological studies associated with infectious diseases related to AGS.
authors.affiliation: Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju, 501-759, Republic of Korea.
authors.forename: Soon-Nang
authors.initials: SN
authors.lastname: Park
authors.forename: Yun Kyong
authors.initials: YK
authors.lastname: Lim
authors.forename: Joong-Ki
authors.initials: JK
authors.lastname: Kook
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013045:Species Specificity; D034366:Streptococcus anginosus / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D012321:DNA-Directed RNA Polymerases