title: Nucleolin identified by comparative mass‑spectra analysis is a potential marker for invasive progression of hepatocellular carcinoma.
abstract: At present, the diagnosis and prognosis of hepatocellular carcinoma (HCC) metastasis remains poor. Recently, a number of proteins associated with the metastasis and invasion of HCC were identified; however, the effective markers require further elucidation. In the current study, a nucleolin expression was observed in MHCC97L and HCCLM9 HCC cell lines, with low and high metastatic potentials respectively, using comparative proteomics. The data indicated that nucleolin expression in the nucleus was significantly higher in HCCLM9 cells, and it primarily influenced the migration of HCC cells in vitro. Thus, to the best of our knowledge this is the first study to hypothesize that nucleolin may be a novel marker for HCC invasive progression.
authors.affiliation: Department of Anesthesiology, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qian
authors.affiliation: Department of Anesthesiology, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Diabetic Center of Control and Prevention, The PLA 520 Hospital, Mianyang, Sichuan 621000, P.R. China.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Hepatobiliary Surgery, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Maolin
authors.initials: M
authors.lastname: Yan
authors.affiliation: Department of Pathology, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Anesthesiology, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Chen
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D018450:Disease Progression; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology; D013058:Mass Spectrometry; D009361:Neoplasm Invasiveness; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012333:RNA, Messenger; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D000097796:Nucleolin
chemical_list: D014408:Biomarkers, Tumor; D010750:Phosphoproteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D000097796:Nucleolin