title: New intracanal formulations containing doxycycline or chlorhexidine against Enterococcus faecalis.
abstract: The present study aims to evaluate the antimicrobial effect of two new intracanal preparations against E. faecalis. Thirty single-rooted human canine teeth were used. The crowns were removed and the roots were instrumented using a conventional technique. Three groups of ten teeth each were infected with 108 CFU/ ml of E. faecalis for 21 days. The root canals were flled with new intracanal medications containing 3% doxycycline hydrochloride (DX) or 2% chlorhexidine digluconate (CHX). Ten teeth received no medication (NM)-negative control. Microbial samples were obtained 21 days after contamination: 14 days under the effect of the intracanal medications and 7 days after replacing the medications by BHI broth. The samples were homogenized, diluted, seeded on BHI agar and incubated for 48h/36°C. The number of colony forming units (CFU/ml) was obtained and analyzed statistically. All intracanal dressings significantly reduced the number of bacterial cells in the root canal after 14 days with medication. After the period with 7 days with BHI broth, the CFU counts of E. faecalis remained at low values. However, the NM group showed a significant increase of CFU in this period to similar values of the initial contamination. 3% doxycycline hydrochloride gel and 2% CHX gel were effective to eliminate E. faecalis from the root canal system.
authors.affiliation: PhD Student, Department of Endodontics, School of Dentistry, University of Porto, Porto, Portugal.
authors.forename: Ana Rita Marques da
authors.initials: AR
authors.lastname: Silva
authors.affiliation: PhD, State University of Ponta Grossa, Postgraduate Program in Dentistry, CEP 84030-900, Uvaranas, Ponta Grossa Paraná Brazil.
authors.forename: Shelon Cristina Souza
authors.initials: SC
authors.lastname: Pinto
authors.affiliation: PhD, State University of Ponta Grossa, Postgraduate Program in Dentistry, CEP 84030-900, Uvaranas, Ponta Grossa Paraná Brazil.
authors.forename: Elizabete Brasil dos
authors.initials: EB
authors.lastname: Santos
authors.affiliation: PhD, State University of Ponta Grossa, Postgraduate Program in Dentistry, CEP 84030-900, Uvaranas, Ponta Grossa Paraná Brazil.
authors.forename: Fábio André dos
authors.initials: FA
authors.lastname: Santos
authors.affiliation: PhD, State University of Ponta Grossa, Postgraduate Program in Dentistry, CEP 84030-900, Uvaranas, Ponta Grossa Paraná Brazil.
authors.forename: Paulo Vitor
authors.initials: PV
authors.lastname: Farago
authors.affiliation: PhD, State University of Ponta Grossa, Postgraduate Program in Dentistry, CEP 84030-900, Uvaranas, Ponta Grossa Paraná Brazil.
authors.forename: João Carlos
authors.initials: JC
authors.lastname: Gomes
authors.affiliation: PhD, Department of Endodontics, School of Dentistry, University of Porto, Porto, Portugal.
authors.forename: Irene
authors.initials: I
authors.lastname: Pina-Vaz
authors.affiliation: PhD, Department of Endodontics, School of Dentistry, University of Porto, Porto, Portugal.
authors.forename: Manuel Fontes
authors.initials: MF
authors.lastname: Carvalho
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D058491:Bacterial Load / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003481:Cuspid / Q000382:microbiology; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004318:Doxycycline / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D012388:Root Canal Irrigants / Q000737:chemistry / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants; C010882:chlorhexidine gluconate; D004318:Doxycycline; D002710:Chlorhexidine