title: Effect of cytochalasin B on 3-O-[14C]-methyl-D-glucose or D-[U-14C]glucose handling by BRIN-BD11 cells.
abstract: The present study aimed to investigate the effects of cytochalasin B (20 μM) on the uptake of 3-O-[14C]-methyl-D-glucose or D-[U-14C]glucose (8.3 mM each) by BRIN-BD11 cells. Taking into account the distribution space of tritiated water (3HOH), which was unexpectedly increased shortly after exposure of the cells to cytochalasin B and then progressively returned to its control values, and that of L-[1-14C]glucose, used as an extracellular marker, it was demonstrated that cytochalasin B caused a modest, but significant inhibition of the uptake of D-glucose and its non-metabolized analog by the BRIN-BD11 cells. These findings resemble those observed in acinar or ductal cells of the rat submaxillary gland and displayed a relative magnitude comparable to that found for the inhibition of D-glucose metabolism by cytochalasin B in purified pancreatic islet B cells. These findings reinforce the view that the primary site of action of cytochalasin B is located at the level of the plasma membrane.
authors.affiliation: Laboratory of Experimental Hormonology, Brussels Free University, Brussels B-1070, Belgium.
authors.forename: Sibel
authors.initials: S
authors.lastname: Cetik
authors.affiliation: Laboratory of Experimental Hormonology, Brussels Free University, Brussels B-1070, Belgium.
authors.forename: Aigun
authors.initials: A
authors.lastname: Rzajeva
authors.affiliation: Laboratory of Experimental Hormonology, Brussels Free University, Brussels B-1070, Belgium.
authors.forename: Willy J
authors.initials: WJ
authors.lastname: Malaisse
authors.affiliation: Laboratory of Experimental Hormonology, Brussels Free University, Brussels B-1070, Belgium.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Sener
keywords: 3-O-[14C]-methyl-D-glucose or D-[U-14C]glucose handling; cytochalasin B; insulin-producing BRIN-BD11 cells