title: Bifidobacterium faecale sp. nov., isolated from human faeces.
abstract: A novel strain, designated strain CU3-7(T), was isolated from faeces of a two-week-old baby. The isolate was Gram-staining-positive, anaerobic and rod-shaped. Results from 16S rRNA gene sequence analysis revealed that strain CU3-7(T) was phylogenetically affiliated with members of the genus Bifidobacterium. Strain CU3-7(T) showed the highest level of sequence similarity with Bifidobacterium adolescentis KCTC 3216(T) (98.4 %), followed by Bifidobacterium ruminantium KCTC 3425(T) (97.9 %). Analysis of hsp60 sequences showed that strain CU3-7(T) was closely related to B. adolescentis KCTC 3216(T) (94.0 %) and B. ruminantium KCTC 3425(T) (92.5 %). The DNA-DNA hybridization values with the closely related strains were all below the cut-off value for species delineation, 17.0 % with B. ruminantium KCTC 3425(T) and 14.9 % with B. adolescentis KCTC 3216(T). Fructose-6-phosphate phosphoketolase activity was detected. The predominant cellular fatty acids were C16 : 0 (27.7 %), C18 : 1ω9c (27.4 %) and C18 : 1ω9c dimethylacetate (15.5 %). The DNA G+C content was 58.6 mol%. On the basis of polyphasic taxonomy, strain CU3-7(T) should be classified as the type strain of a novel species within the genus Bifidobacterium, for which the name Bifidobacterium faecale sp. nov. is proposed ( = KACC 17904(T) = JCM 19861(T)).
authors.affiliation: Department of Systems Biotechnology, Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: Jung-Hye
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Lee
authors.affiliation: Fermentation and Functionality Research Group, Korea Food Research Institute, Sungnam 463-746, Republic of Korea.
authors.forename: Myung-Ki
authors.initials: MK
authors.lastname: Lee
authors.affiliation: Department of Systems Biotechnology, Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Cha
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: Geun-Bae
authors.initials: GB
authors.lastname: Kim
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001644:Bifidobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018834:Chaperonin 60; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D000446:Aldehyde-Lyases; C065556:fructose-6-phosphate phosphoketolase