title: Characterization of endolithic cyanobacterial strain, Leptolyngbya sp. ISTCY101, for prospective recycling of CO₂ and biodiesel production.
abstract: The present investigation evaluates the potential of an endolithic cyanobacterium isolated from marble rock to utilize sodium bicarbonate (NaHCO₃) as carbon source for prospective recycling of CO₂ into biodiesel. Microalgae thriving on marble were cultured and subjected to increasing NaHCO₃ concentration. The most competent isolate was identified and characterized in terms of growth, lipid content and fatty acid profile. A semicontinuous mesh incubator was designed for biofilm development. Isolate ISTCY101 was identified as Leptolyngbya sp. by 16S rRNA sequencing. Leptolyngbya ISTCY101 efficiently used BG-11 (50 mM NaHCO₃) and artificial seawater medium (25 gL(-1) NaCl) with biomass productivity 78.9 and 75.74 mg L(-1)d(-1), respectively. Maximum areal biomass productivity of 2.01 gm(-2)d(-1) was recorded in the mesh incubator, with complete exclusion of centrifugation for harvesting. Lipid content varied from 16% to 21%, consisting predominantly of C16:0, C16:1, C18:0, C18:1 fatty acids (>60%) making promising feedstock for biodiesel production.
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India. Electronic address: singhjyoti22@gmail.com.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Singh
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
authors.forename: Ritu
authors.initials: R
authors.lastname: Tripathi
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India. Electronic address: isthakur@mail.jnu.ac.in.
authors.forename: Indu Shekhar
authors.initials: IS
authors.lastname: Thakur
mesh_terms: D056804:Biofuels; D002245:Carbon Dioxide / Q000378:metabolism*; D003246:Conservation of Energy Resources; D000458:Cyanobacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D050356:Lipid Metabolism; D059027:Recycling
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D002245:Carbon Dioxide
keywords: Biodiesel; Cyanobacterium; Endolithic; Marble rock; Sodium bicarbonate