title: Zingiberene attenuates hydrogen peroxide-induced toxicity in neuronal cells.
abstract: In this experimental design, we explored the neuroprotective potential of zingiberene (ZGB), a monocyclic sesquiterpene, in hydrogen peroxide (H2O2)-induced toxicity in newborn rat cerebral cortex cell cultures for the first time. The rats were exposed to H2O2 for 6 h to determine the oxidative stress levels. To evaluate cell viability, both 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were carried out. Total antioxidant capacity (TAC) and total oxidative stress (TOS) parameters were used to evaluate oxidative changes. Besides determining 8-hydroxy-2-deoxyguanosine (8-OH-dG) levels in vitro, single-cell gel electrophoresis was also performed to measure the resistance of neuronal DNA to H2O2- exposed rats. Our results showed that survival and TAC levels of the cells decreased, while TOS, 8-OH-dG levels and the mean values of the total scores of cells showing DNA damage increased in the H2O2 alone-treated cultures. But pretreatment of ZGB suppressed the cytotoxicity, genotoxicity and oxidative stress that were increased by H2O2. Based on these observations, it is suggested that the sesquiterpene ZGB can be used as a novel and natural potential therapeutic in counteracting oxidative damages in the field of neurodegenerative disorders.
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey basaktogar@gmail.com.
authors.forename: B
authors.initials: B
authors.lastname: Togar
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Türkez
authors.affiliation: Department of Pharmacology, G. D'Annunzio University, Chieti, Italy.
authors.forename: A D
authors.initials: AD
authors.lastname: Stefano
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Tatar
authors.affiliation: Department of Medical Pharmacology, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
authors.forename: D
authors.initials: D
authors.lastname: Cetin
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D020552:Comet Assay; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D000081222:Monocyclic Sesquiterpenes; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012717:Sesquiterpenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D000081222:Monocyclic Sesquiterpenes; D012717:Sesquiterpenes; D000080242:8-Hydroxy-2'-Deoxyguanosine; C477983:zingiberene; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D003849:Deoxyguanosine
keywords: DNA damage; H2O2; Zingiberene; cultured neuron; neuroprotective action; oxidative stress