title: Effect of anti-Mullerian hormone in culture medium on quality of mouse oocytes matured in vitro.
abstract: Anti-mullerian hormone (AMH) is thought to reflect the growth of follicles and the ovarian function. However, the role of AMH in culture medium during in vitro maturation (IVM) on oocyte quality and subsequent development potential is unclear. The objective of this study is to investigate the effect of recombinant human AMH (rh-AMH) supplemented into IVM medium on oocyte quality. Cumulus-oocyte complexes (COCs) were obtained from ICR mice and cultured in vitro with the different concentrations (0-1,000 ng/ml) of rh-AMH. Following 16-18 h of culture, quantitative PCR and ELISA were performed to analyze GDF9 and BMP15 mRNA expression and protein production from the oocytes. Subsequently, in vitro fertilization (IVF) and early embryonic development were employed to further evaluate the quality of in vitro matured oocytes. The results showed that AMH was only expressed in cumulus cells but not in the oocytes. However, AMH most specific receptor, AMHR-II, was expressed in both oocytes and cumulus cells. The levels of GDF9 and BMP15 expression and blastocyst formation rate were significantly increased (p<0.05) when the IVM medium was supplemented with 100 ng/ml of rh-AMH. With AdH1-SiRNA/AMH for knocking down of AMH expression during IVM significantly reduced (p<0.05) the levels of GDF9 and BMP15 expression and blastocysts formation rate. These results suggest that AHM improves oocytes quality by up-regulating GDF9 and BMP15 expressions during IVM.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Yihui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Yigui
authors.initials: Y
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Jiayin
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China; Division of Research, Department of Obstetrics and Gynecology, McGill University, Montreal, Canada.
authors.forename: Ri-Cheng
authors.initials: RC
authors.lastname: Chian
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000008:administration & dosage / Q000096:biosynthesis / Q000494:pharmacology*; D001755:Blastocyst / Q000187:drug effects; D055430:Bone Morphogenetic Protein 15 / Q000096:biosynthesis / Q000235:genetics; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D054885:Cumulus Cells / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D055785:Gene Knockdown Techniques; D055429:Growth Differentiation Factor 9 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000494:pharmacology; D018000:Receptors, Peptide / Q000096:biosynthesis / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: C525902:Bmp15 protein, mouse; D055430:Bone Morphogenetic Protein 15; D003470:Culture Media; C525898:Gdf9 protein, mouse; D055429:Growth Differentiation Factor 9; D034741:RNA, Small Interfering; D018000:Receptors, Peptide; D018125:Receptors, Transforming Growth Factor beta; D011994:Recombinant Proteins; C084621:anti-Mullerian hormone receptor; D054304:Anti-Mullerian Hormone