title: Concentration, characterization and application of lipases from Sporidiobolus pararoseus strain.
abstract: Lipases produced by a newly isolated Sporidiobolus pararoseus strain have potential catalytic ability for esterification reactions. After production, the enzymatic extracts (conventional crude and precipitated, 'CC' and 'CP', and industrial crude and precipitated, 'IC' e 'IP') were partially characterized. The enzymes presented, in general, higher specificity for short chain alcohols and fatty acids. The precipitated extract showed a good thermal stability, higher than that for crude enzymatic extracts. The 'CC' and 'CP' enzymes presented high activities after exposure to pH 6.5 and 40 °C. On the other hand, the 'IC' and 'IP' extracts kept their activities in a wide range of pH memory but presented preference for higher reaction temperatures. Preliminary studies of application of the crude lipase extract in the enzymatic production of geranyl propionate using geraniol and propionic acid as substrates in solvent-free system led to a reaction conversion of 42 ± 1.5%.
authors.affiliation: Departamento de Engenharia de Alimentos Universidade Regional Integrada do Alto Uruguai e das Missões Campus de Erechim, ErechimRS Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Smaniotto
authors.affiliation: Departamento de Engenharia de Alimentos Universidade Regional Integrada do Alto Uruguai e das Missões Campus de Erechim, ErechimRS Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Skovronski
authors.affiliation: Departamento de Engenharia de Alimentos Universidade Regional Integrada do Alto Uruguai e das Missões Campus de Erechim, ErechimRS Brazil.
authors.forename: Elisandra
authors.initials: E
authors.lastname: Rigo
authors.affiliation: Laboratório de Biologia Celular e Molecular Universidade de São Paulo PiracicabaSP Brazil.
authors.forename: Siu Mui
authors.initials: SM
authors.lastname: Tsai
authors.affiliation: Laboratório de Biologia Celular e Molecular Universidade de São Paulo PiracicabaSP Brazil.
authors.forename: Ademir
authors.initials: A
authors.lastname: Durrer
authors.affiliation: Laboratório de Biologia Celular e Molecular Universidade de São Paulo PiracicabaSP Brazil.
authors.forename: Lillian Liva
authors.initials: LL
authors.lastname: Foltran
authors.affiliation: Departamento de Engenharia de Alimentos Universidade Regional Integrada do Alto Uruguai e das Missões Campus de Erechim, ErechimRS Brazil.
authors.forename: Natália
authors.initials: N
authors.lastname: Paroul
authors.affiliation: Departamento de Engenharia Química e de Alimentos Universidade Federal de Santa Catarina Campus Universitário FlorianópolisSC Brazil.
authors.forename: Marco
authors.initials: M
authors.lastname: Di Luccio
authors.affiliation: Departamento de Engenharia Química e de Alimentos Universidade Federal de Santa Catarina Campus Universitário FlorianópolisSC Brazil.
authors.forename: J Vladimir
authors.initials: JV
authors.lastname: Oliveira
authors.affiliation: Departamento de Engenharia Química e de Alimentos Universidade Federal de Santa Catarina Campus Universitário FlorianópolisSC Brazil.
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
authors.affiliation: Universidade Federal da Fronteira Sul Campus de Erechim ErechimRS Brazil.
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
mesh_terms: D000438:Alcohols / Q000378:metabolism; D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D004795:Enzyme Stability; D005227:Fatty Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D000438:Alcohols; D005227:Fatty Acids; D008049:Lipase
keywords: Sporidiobolus pararoseus; enzyme characterization; submerged fermentation; ‘synthetic lipase’