title: Molecular mechanism of acute radiation enteritis revealed using proteomics and biological signaling network analysis in rats.
abstract: OBJECTIVE
  Radiation enteritis (RE) has emerged as a significant complication that can progress to severe gastrointestinal disease and the mechanisms underlying its genesis remain poorly understood. The aim of this study was to identify temporal changes in protein expression potentially associated with acute inflammation and to elucidate the mechanism underlying radiation enteritis genesis.
  
  
  METHODS
  Male Sprague-Dawley rats were irradiated in the abdomen with a single dose of 10 Gy to establish an in vivo model of acute radiation enteritis. Two-dimensional fluorescence difference gel electrophoresis, matrix-assisted laser desorption/ionization time-of-flight spectrometer (MALDI-TOF) tandem mass spectrometry, and peptide mass fingerprinting were used to determine differentially expressed proteins between normal and inflamed intestinal mucosa. Additionally, differentially expressed proteins were evaluated by KO Based Annotation System to find the biological functions associated with acute radiation enteritis.
  
  
  RESULTS
  Intensity changes of 86 spots were detected with statistical significance (ratio ≥ 1.5 or ≤ 1.5, P < 0.05). Sixty one of the 86 spots were identified by MALDI-TOF/TOF tandem mass spectrometry. These radiation-induced proteins with biological functions showed that the FAS pathway and glycolysis signaling pathways were significantly altered using the KOBAS tool.
  
  
  CONCLUSIONS
  Our results reveal an underlying mechanism of radiation-induced acute enteritis, which may help clarify the pathogenesis of RE and point to potential targets for therapeutic interventions.
authors.affiliation: Gastrointestinal Institute of Sun Yat-Sen University, The Sixth Affiliated Hospital of Sun Yat-Sen University, 26 Yuancunerheng Road, Guangzhou, 510655, People's Republic of China.
authors.forename: Shunxin
authors.initials: S
authors.lastname: Song
authors.forename: Dianke
authors.initials: D
authors.lastname: Chen
authors.forename: Tenghui
authors.initials: T
authors.lastname: Ma
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Luo
authors.forename: Zuli
authors.initials: Z
authors.lastname: Yang
authors.forename: Daohai
authors.initials: D
authors.lastname: Wang
authors.forename: Xinjuan
authors.initials: X
authors.lastname: Fan
authors.forename: Qiyuan
authors.initials: Q
authors.lastname: Qin
authors.forename: Beibei
authors.initials: B
authors.lastname: Ni
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Guo
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Xian
authors.forename: Ping
authors.initials: P
authors.lastname: Lan
authors.forename: Xinping
authors.initials: X
authors.lastname: Cao
authors.forename: Mingtao
authors.initials: M
authors.lastname: Li
authors.forename: Jianping
authors.initials: J
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004751:Enteritis / Q000209:etiology* / Q000378:metabolism; D008297:Male; D053858:Metabolic Networks and Pathways / Q000528:radiation effects*; D040901:Proteomics; D011833:Radiation Injuries, Experimental; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000502:physiology*