title: Erratum: Differential expressed genes in ECV304 Endothelial-like Cells infected with Human Cytomegalovirus.
abstract: BACKGROUND
  Human cytomegalovirus (HCMV) is a virus which has the potential to alter cellular gene expression through multiple mechanisms.
  
  
  OBJECTIVE
  With the application of DNA microarrays, we could monitor the effects of pathogens on host-cell gene expression programmes in great depth and on a broad scale.
  
  
  METHODS
  Changes in mRNA expression levels of human endothelial-like ECV304 cells following infection with human cytomegalovirus AD169 strain was analyzed by a microarray system comprising 21073 60-mer oligonucleotide probes which represent 18716 human genes or transcripts.
  
  
  RESULTS
  The results from cDNA microarray showed that there were 559 differential expressed genes consisted of 471 upregulated genes and 88 down-regulated genes. Real-time qPCR was performed to validate the expression of 6 selected genes (RPS24, MGC8721, SLC27A3, MST4, TRAF2 and LRRC28), and the results of which were consistent with those from the microarray. Among 237 biology processes, 39 biology processes were found to be related significantly to HCMV-infection. The signal transduction is the most significant biological process with the lowest p value (p=0.005) among all biological process which involved in response to HCMV infection.
  
  
  CONCLUSIONS
  Several of these gene products might play key roles in virus-induced pathogenesis. These findings may help to elucidate the pathogenic mechanisms of HCMV caused diseases. [This corrects the article on p. 243 in vol. 13, PMID: 24235919.].
authors.affiliation: Department of Clinical Laborotary Science, Guiyang Medical College, Guiyang, Guizhou 550004, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Ma
authors.affiliation: The Center for Heart Development, Key Lab of National Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410006 China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Mo
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Haiquan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Guangdong Province Center of Disease Control Virology Section, Guangzhou, Guangdong 510033, China.
authors.forename: Huanying
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Guangdong Province Center of Disease Control Virology Section, Guangzhou, Guangdong 510033, China.
authors.forename: Changwen
authors.initials: C
authors.lastname: Ke
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Wenling
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Experimental Surgery, Tangdu Hospital, The Forth Military Medical University, Xi'an, Shannxi, 710038, China.
authors.forename: Yanyang
authors.initials: Y
authors.lastname: Tu
authors.affiliation: Department of Experimental Surgery, Tangdu Hospital, The Forth Military Medical University, Xi'an, Shannxi, 710038, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhang
keywords: Gene expression profiling; Human cytomegalovirus; infectomics; microarray