title: Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas.
abstract: OBJECTIVE
  To investigate the effect of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.
  
  
  METHODS
  Overexpression or downregulation of mesothelin expression in INS-1 cells was carried out to investigate the effect of mesothelin during cell proliferation and cell apoptosis in vitro. Adenovirus-mediated RNA interference was performed to block mesothelin in vivo to directly assess the role of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.
  
  
  RESULTS
  Exogenous overexpression of mesothelin promoted cell proliferation, cell colony formation and enhanced cell resistance to apoptosis of INS-1 cells. Down-regulation of mesothelin made no difference in cell proliferation and apoptosis compared with that in the control group. After an injection of adenovirus-mesothelin, a significantly increased number of small islets appeared, and the expression of PCNA was decreased on day 7 and day 14 compared with the Ad-EGFP group.
  
  
  CONCLUSIONS
  Mesothelin was able to promote β cell proliferation in the remodeling stage of neonatal rats. Mesothelin may have an important role in the remodeling of the endocrine pancreas in neonatal rats.
authors.affiliation: Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Yin
authors.affiliation: Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.
authors.forename: Liang-Hui
authors.initials: LH
authors.lastname: You
authors.affiliation: Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.
authors.forename: Qing-Xin
authors.initials: QX
authors.lastname: Yuan
authors.affiliation: Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.
authors.forename: Xiao-Di
authors.initials: XD
authors.lastname: Liang
authors.affiliation: Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.
authors.forename: Lin-Tao
authors.initials: LT
authors.lastname: Wang
authors.affiliation: Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.
authors.forename: Ke-Ming
authors.initials: KM
authors.lastname: Wang
authors.affiliation: Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: De
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D007515:Islets of Langerhans / Q000254:growth & development / Q000378:metabolism*; D000090204:Mesothelin; D011247:Pregnancy; D034622:RNA Interference; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: D058851:GPI-Linked Proteins; D000090204:Mesothelin
keywords: Cell proliferation; Mesothelin; Pancreatic development; Pancreatic remodeling; Small islets