title: Clickable Cγ-azido(methylene/butylene) peptide nucleic acids and their clicked fluorescent derivatives: synthesis, DNA hybridization properties, and cell penetration studies.
abstract: Synthesis, characterization, and DNA complementation studies of clickable C(γ)-substituted methylene (azm)/butylene (azb) azido PNAs show that these analogues enhance the stability of the derived PNA:DNA duplexes. The fluorescent PNA oligomers synthesized by their click reaction with propyne carboxyfluorescein are seen to accumulate around the nuclear membrane in 3T3 cells.
authors.affiliation: Chemical Biology Unit, Indian Institute of Science Education and Research , Dr Homi Bhabha Road, Pune 411008, India.
authors.forename: Deepak R
authors.initials: DR
authors.lastname: Jain
authors.forename: Krishna N
authors.initials: KN
authors.lastname: Ganesh
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry*; D002467:Cell Nucleus / Q000737:chemistry*; D057930:Click Chemistry; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D051379:Mice; D018613:Microscopy, Confocal; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics
chemical_list: D005456:Fluorescent Dyes; D020135:Peptide Nucleic Acids; D004247:DNA