title: An acute exposure to glyphosate-based herbicide alters aromatase levels in testis and sperm nuclear quality.
abstract: Roundup is the major pesticide used in agriculture worldwide; it is a glyphosate-based herbicide. Its molecular effects are studied following an acute exposure (0.5%) of fifteen 60-day-old male rats during an 8-day period. Endocrine (aromatase, estrogen and androgen receptors, Gper1 in testicular and sperm mRNAs) and testicular functions (organ weights, sperm parameters and expression of the blood-testis barrier markers) were monitored at days 68, 87, and 122 after treatment, spermiogenesis and spermatogenesis. The major disruption is an increase of aromatase mRNA levels at least by 50% in treated rats at all times, as well as the aromatase protein. We have also shown a similar increase of Gper1 expression at day 122 and a light modification of BTB markers. A rise of abnormal sperm morphology and a decrease of the expression of protamine 1 and histone 1 testicular in epididymal sperm are observed despite a normal sperm concentration and motility.
authors.affiliation: University of Caen, Institute of Biology, Network on Risks MRSH-CNRS, Esplanade de la Paix, 14032 Caen Cedex, France; CRIIGEN, 40 rue de Monceau, 75008 Paris, France. Electronic address: estelle.cassault-meyer@unicaen.fr.
authors.forename: Estelle
authors.initials: E
authors.lastname: Cassault-Meyer
authors.affiliation: University of Caen, Institute of Biology, Network on Risks MRSH-CNRS, Esplanade de la Paix, 14032 Caen Cedex, France; CRIIGEN, 40 rue de Monceau, 75008 Paris, France. Electronic address: steeve-gress@hotmail.fr.
authors.forename: Steeve
authors.initials: S
authors.lastname: Gress
authors.affiliation: University of Caen, Institute of Biology, Network on Risks MRSH-CNRS, Esplanade de la Paix, 14032 Caen Cedex, France; CRIIGEN, 40 rue de Monceau, 75008 Paris, France. Electronic address: criigen@unicaen.fr.
authors.forename: Gilles-Éric
authors.initials: GÉ
authors.lastname: Séralini
authors.affiliation: University of Caen, Institute of Biology, Network on Risks MRSH-CNRS, Esplanade de la Paix, 14032 Caen Cedex, France. Electronic address: idenis1@orange.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Galeraud-Denis
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics*; D002467:Cell Nucleus / Q000187:drug effects; D004822:Epididymis / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D006657:Histones / Q000235:genetics; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011479:Protamines / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000235:genetics; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D000097797:Glyphosate
chemical_list: D001141:Aromatase; D005998:Glycine; D006540:Herbicides; D006657:Histones; D011479:Protamines; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D000097797:Glyphosate; C520305:Gper1 protein, rat; C513174:Prm1 protein, rat
keywords: Aromatase (P450 arom); Glyphosate; Nuclear quality; Roundup; Spermatozoa; Testis