title: The challenge of producing ubiquitinated proteins for structural studies.
abstract: Protein ubiquitination is an important post-translational modification involved in several essential signalling pathways. It has different effects on the target protein substrate, i.e., it can trigger the degradation of the protein in the proteasome, change the interactions of the modified protein with its partners, or affect its localization and activity. In order to understand the molecular mechanisms underlying the consequences of protein ubiquitination, scientists have to face the challenging task of producing ubiquitinated proteins for structural characterization with X-ray crystallography and/or nuclear magnetic resonance (NMR) spectroscopy. These techniques require milligrams of homogeneous samples of high purity. The strategies proposed so far for the production of ubiquitinated proteins can be divided into two groups, i.e., chemical (or non-enzymatic) and enzymatic methodologies. In this review, we summarize the still very sparse examples available in the literature that describe successful production of ubiquitinated proteins amenable for biochemical and structural studies, and discuss advantages and disadvantages of the techniques proposed. We also give a perspective of the direction in which the field might evolve.
authors.affiliation: National Institute for Medical Research, MRC, The Ridgeway, NW71AA London, UK. sfaggia@nimr.mrc.ac.uk.
authors.forename: Serena
authors.initials: S
authors.lastname: Faggiano
authors.affiliation: Department of Clinical Neuroscience, King's College London, Denmark Hill Campus, SE58AF London, UK. apastor@nimr.mrc.ac.uk.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pastore