title: Package of NDV-pseudotyped HIV-Luc virus and its application in the neutralization assay for NDV infection.
abstract: Newcastle disease virus (NDV) is a member of the Paramyxovirinae subfamily and can infect most species of birds. It has been a great threat for the poultry industry all around the world. In this report, we successfully produced infectious pseudotyped pNL4-3-Luc-R(-)E(-) (HIV-Luc) viruses with the HN and F envelope proteins of NDV. Further investigation revealed the cytoplasmic domains of HN and F, especially HN, plays a significant role in the infection efficiency of these pseudotyped HIV-Luc viruses. Replacement of, or direct fusion to the cytoplasmic domain of the HN protein by that of vesicular stomatitis virus G (VSV-G) could greatly enhance or destroy the infective potential of HN and F-pseudotyped (NDV-pseudotyped) HIV-Luc virus. We further established a novel neutralization assay to evaluate neutralizing antibodies against NDV with the NDV-pseudotyped HIV-Luc viruses. Comparative neutralization data indicate that the results determined by using the NDV-pseudotyped HIV-Luc viruses are as reliable as those by the conventional virus-neutralization assay (VN test) with native NDV. Moreover, the results show that the novel neutralization assay is more sensitive than the VN test.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China; College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.
authors.forename: Peixin
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China; College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Si
authors.affiliation: College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Xiu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.
authors.forename: Henggui
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000502:physiology; D000914:Antibodies, Viral / Q000502:physiology; D001717:Birds; D017930:Genes, Reporter; D057809:HEK293 Cells; D015497:HIV-1 / Q000235:genetics*; D015707:HN Protein / Q000235:genetics; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D009500:Neutralization Tests; D009521:Newcastle Disease / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000235:genetics
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015707:HN Protein; D007106:Immune Sera; D014760:Viral Fusion Proteins; D008156:Luciferases