title: Specific maltose derivatives modulate the swarming motility of nonswarming mutant and inhibit bacterial adhesion and biofilm formation by Pseudomonas aeruginosa.
abstract: We have demonstrated that specific synthetic maltose derivatives activate the swarming motility of a Pseudomonas aeruginosa nonswarming mutant (rhlA) at low concentration, but inhibit it at high concentration. Although these molecules are not microbicidal, active maltose derivatives with bulky hydrocarbon groups inhibited bacterial adhesion, and exhibited biofilm inhibition and dispersion (IC50 ~20 μM and DC50 ~30 μM, respectively). Because the swarming motility of the rhlA mutant is abolished by the lack natural rhamnolipids, the swarming activation suggests that maltose derivatives are analogues of rhamnolipids. Together, these results suggest a new approach of controlling multiple bacterial activities (bacterial adhesion, biofilm formation, and swarming motility) by a set of disaccharide-based molecules.
authors.affiliation: Department of Chemistry, Syracuse University, Syracuse, New York 13244 (USA).
authors.forename: Gauri S
authors.initials: GS
authors.lastname: Shetye
authors.forename: Nischal
authors.initials: N
authors.lastname: Singh
authors.forename: Changqing
authors.initials: C
authors.lastname: Jia
authors.forename: Chan D K
authors.initials: CD
authors.lastname: Nguyen
authors.forename: Guirong
authors.initials: G
authors.lastname: Wang
authors.forename: Yan-Yeung
authors.initials: YY
authors.lastname: Luk
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D005798:Genes, Bacterial; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008320:Maltose / Q000031:analogs & derivatives* / Q000494:pharmacology; D009154:Mutation; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D006017:Glycolipids; C418382:rhamnolipid; D008320:Maltose
keywords: Pseudomonas aeruginosa; biofilm inhibition; disaccharide hydrocarbons; inhibitors; swarming motility