title: Efficacy of tumor-targeting Salmonella typhimurium A1-R on nude mouse models of metastatic and disseminated human ovarian cancer.
abstract: We report here the efficacy of tumor-targeting Salmonella typhimurium A1-R (A1-R) on mouse models of disseminated and metastatic ovarian cancer. The proliferation-inhibitory efficacy of A1-R on human ovarian cancer cell lines (SKOV-3-GFP, OVCAR-3-RFP) was initially demonstrated in vitro. Orthotopic and dissemination mouse models of ovarian cancer were made with the human ovarian cancer cell line SKOV-3-GFP. After tumor implantation, the mice were treated with A1-R (5 × 10(7)  colony-forming units [CFU], i.v.), and there were no severe adverse events observed. In the orthotopic model, tumor volume after treatment was 276 ± 60.8 mm(3), compared to 930 ± 342 mm(3) in the untreated control group (P = 0.022). There was also a significant difference in survival between treated mice and untreated mice in a peritoneal dissemination model (P = 0.005). The results of this report demonstrate that A1-R is effective for highly aggressive human ovarian cancer in metastatic and dissemination mouse models and suggest its clinical potential for this highly treatment-resistant disease.
authors.affiliation: AntiCancer, Inc., San Diego, California; Department of Surgery, University of California San Diego, San Diego, California; Department of Frontier Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Shinji
authors.initials: S
authors.lastname: Miwa
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Hiroshima
authors.forename: Shuya
authors.initials: S
authors.lastname: Yano
authors.forename: Fuminari
authors.initials: F
authors.lastname: Uehara
authors.forename: Mako
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Toneri
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.forename: Hisahiro
authors.initials: H
authors.lastname: Matsubara
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000473:pathology / Q000628:therapy*; D010534:Peritoneal Neoplasms / Q000473:pathology / Q000556:secondary* / Q000628:therapy*; D012480:Salmonella Infections / Q000473:pathology*; D012486:Salmonella typhimurium / Q000145:classification / Q000472:pathogenicity*; D016019:Survival Analysis; D023041:Xenograft Model Antitumor Assays
keywords: BACTERIA; GFP; IMAGING; NUDE MICE; OVARIAN CANCER; Salmonella typhimurium; TUMOR-TARGETING