title: Scanning inverse fluorescence correlation spectroscopy.
abstract: Scanning Inverse Fluorescence Correlation Spectroscopy (siFCS) is introduced to determine the absolute size of nanodomains on surfaces. We describe here equations for obtaining the domain size from cross- and auto-correlation functions, measurement simulations which enabled testing of these equations, and measurements on model surfaces mimicking membranes containing nanodomains. Using a confocal microscope of 270 nm resolution the size of 250 nm domains were estimated by siFCS to 257 ± 12 nm diameter, and 40 nm domains were estimated to 65 ± 26 nm diameter. Applications of siFCS for sizing of nanodomains and protein clusters in cell membranes are discussed.
authors.forename: Jan
authors.initials: J
authors.lastname: Bergstrand
authors.forename: Daniel
authors.initials: D
authors.lastname: Rönnlund
authors.forename: Jerker
authors.initials: J
authors.lastname: Widengren
authors.forename: Stefan
authors.initials: S
authors.lastname: Wennmalm
mesh_terms: D002462:Cell Membrane / Q000648:ultrastructure; D005453:Fluorescence; D006801:Humans; D013050:Spectrometry, Fluorescence / Q000379:methods*