title: Hepatoprotective effect of silymarin on di(2-ethylhexyl)phthalate (DEHP) induced injury in liver FL83B cells.
abstract: Di(2-ethylhexyl)phthalate (DEHP), is a toxic environmental pollutant in our life which can contaminate air, water, and soil. The hepatoprotective effect of silymarin on DEHP-induced injury in FL83B mouse liver cells was investigated by analyzing the cell viability, lactate dehydrogenase (LDH), alanine aminotransferase (ALT), cell cycle arrest, and cell morphology. The results revealed that cell viability decreased while released LDH and ALT increased with the increase of DEHP concentrations. Moreover, cell population of sub-G1 and S phase increased as the concentrations of DEHP increased. Silymarin at 25 μM achieved the highest hepatoprotective effect and exhibited 79% cell viability while only 46% cell viability was found in DEHP injured control. It was also found to reduce LDH release and cell populations of sub-G1 and S phase. Therefore, silymarin could ameliorate DEHP-induced injury and have potential to be further developed as a natural ingredient of health food against phthalate plasticizers induced liver injury.
authors.affiliation: Department of Food Science, National Pingtung University of Science and Technology, No. 1, Hsueh Fu Road, Nei-Pu Hsiang, Pingtung 91201, Taiwan.
authors.forename: Diana
authors.initials: D
authors.lastname: Lo
authors.affiliation: Life Science Center, Hsing Wu University, No. 101, Sec. 1, Fen-Liao Road, Lin Kou, Taipei 244, Taiwan. Electronic address: yuhtai@yahoo.com.
authors.forename: Yuh-Tai
authors.initials: YT
authors.lastname: Wang
authors.affiliation: Department of Food Science, National Pingtung University of Science and Technology, No. 1, Hsueh Fu Road, Nei-Pu Hsiang, Pingtung 91201, Taiwan. Electronic address: globalizationwu@yahoo.com.tw.
authors.forename: Ming-Chang
authors.initials: MC
authors.lastname: Wu
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism; D004051:Diethylhexyl Phthalate / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology; D051379:Mice; D010968:Plasticizers / Q000633:toxicity*; D020011:Protective Agents / Q000494:pharmacology*; D012838:Silymarin / Q000494:pharmacology*
chemical_list: D004785:Environmental Pollutants; D010968:Plasticizers; D020011:Protective Agents; D012838:Silymarin; D004051:Diethylhexyl Phthalate; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Di(2-ethylhexyl)phthalate; FL83B cells; Hepatoprotective; Liver injury; Silymarin