title: Direct and fast detection of Alexandrium minutum algae by using high frequency microbalance.
abstract: In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cell concentration. This simple system is potentially promising for environmental monitoring purposes.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 8235, Laboratoire Interfaces et Systèmes Electrochimiques, F-75252 Paris, France; CNRS, UMR 8235, LISE, F-75005 Paris, France; Universidade Federal do Rio de Janeiro, Instituto de Química, Av. Athos da Silveira Ramos, 149, CT, Bl. A, sala 408, Ilha da Cidade Universitária, CEP: 21.941-909 Rio de Janeiro, RJ, Brazil.
authors.forename: Célia
authors.initials: C
authors.lastname: Sousa
authors.affiliation: IFREMER, Centre de Brest, Laboratoire Détection, Capteurs et Mesures, F-29280 Plouzané, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Compère
authors.affiliation: IFREMER, Centre de Brest, Laboratoire Détection, Capteurs et Mesures, F-29280 Plouzané, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Dreanno
authors.affiliation: IFREMER, Centre de Brest, Laboratoire Détection, Capteurs et Mesures, F-29280 Plouzané, France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Crassous
authors.affiliation: CEA Marcoule, Direction des Sciences du Vivant, iBEB/SBTN/LDCAE, F-30200 Bagnols sur Cèze, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Gas
authors.affiliation: CEA Marcoule, Direction des Sciences du Vivant, iBEB/SBTN/LDCAE, F-30200 Bagnols sur Cèze, France.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Baus
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 8235, Laboratoire Interfaces et Systèmes Electrochimiques, F-75252 Paris, France; CNRS, UMR 8235, LISE, F-75005 Paris, France. Electronic address: hubert.perrot@upmc.fr.
authors.forename: Hubert
authors.initials: H
authors.lastname: Perrot
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D000941:Antigens / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004141:Dinoflagellida / Q000737:chemistry / Q000302:isolation & purification*; D058525:Quartz Crystal Microbalance Techniques / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens
keywords: Alexandrium minutum; Immunosensor; Microalgae; Quartz microbalance