title: The involvement of MiR-1-clathrin pathway in the regulation of phagocytosis.
abstract: Phagocytosis, one of the most powerful immune responses, is a complicated process regulated by many factors. However the regulation of phagocytosis mediated by microRNAs has not been extensively investigated. To address this issue, the regulation of phagocytosis by miR-1 was characterized in this study. The results showed that miR-1 played an important role in the phagocytosis regulation in shrimp in vivo. The sequence analysis indicated that miR-1 was highly conserved from invertebrates to mammals, suggesting that miR-1 might share the similar or same functions in phagocytosis of shrimp hemocytes and mammalian macrophages. The data presented that miR-1 was significantly downregulated in cancerous macrophage RAW264.7 cells compared with those in the isolated murine macrophage and in the immortalized macrophage ANA-1. The findings showed that miR-1 had a great effect on the regulation of phagocytosis in cancerous macrophage by the inhibition of clathrin heavy chain 1 (CLTC1) gene. Therefore our study presented a novel miR-1-mediated regulation of phagocytosis both in invertebrate and in vertebrate.
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou, The People's Republic of China.
authors.forename: Cuilian
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou, The People's Republic of China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou, The People's Republic of China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D033922:Clathrin Heavy Chains / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D003445:Crustacea / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D007313:Insecta / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D010587:Phagocytosis
chemical_list: D035683:MicroRNAs; D033922:Clathrin Heavy Chains