title: New procedure of selected biogenic amines determination in wine samples by HPLC.
abstract: A new procedure for determination of biogenic amines (BA): histamine, phenethylamine, tyramine and tryptamine, based on the derivatization reaction with 2-chloro-1,3-dinitro-5-(trifluoromethyl)-benzene (CNBF), is proposed. The amines derivatives with CNBF were isolated and characterized by X-ray crystallography and (1)H, (13)C, (19)F NMR spectroscopy in solution. The novelty of the procedure is based on the pure and well-characterized products of the amines derivatization reaction. The method was applied for the simultaneous analysis of the above mentioned biogenic amines in wine samples by the reversed phase-high performance liquid chromatography. The procedure revealed correlation coefficients (R(2)) between 0.9997 and 0.9999, and linear range: 0.10-9.00 mg L(-1) (histamine); 0.10-9.36 mg L(-1) (tyramine); 0.09-8.64 mg L(-1) (tryptamine) and 0.10-8.64 mg L(-1) (phenethylamine), whereas accuracy was 97%-102% (recovery test). Detection limit of biogenic amines in wine samples was 0.02-0.03 mg L(-1), whereas quantification limit ranged 0.05-0.10 mg L(-1). The variation coefficients for the analyzed amines ranged between 0.49% and 3.92%. Obtained BA derivatives enhanced separation the analytes on chromatograms due to the inhibition of hydrolysis reaction and the reduction of by-products formation.
authors.affiliation: Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7 Street, Toruń 87-100, Poland.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Piasta
authors.affiliation: Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7 Street, Toruń 87-100, Poland. Electronic address: aj@chem.uni.torun.pl.
authors.forename: Aneta
authors.initials: A
authors.lastname: Jastrzębska
authors.affiliation: Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7 Street, Toruń 87-100, Poland.
authors.forename: Marek P
authors.initials: MP
authors.lastname: Krzemiński
authors.affiliation: Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7 Street, Toruń 87-100, Poland.
authors.forename: Tadeusz M
authors.initials: TM
authors.lastname: Muzioł
authors.affiliation: Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7 Street, Toruń 87-100, Poland.
authors.forename: Edward
authors.initials: E
authors.lastname: Szłyk
mesh_terms: D001555:Benzene Derivatives / Q000737:chemistry; D001679:Biogenic Amines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D018360:Crystallography, X-Ray; D006632:Histamine / Q000032:analysis; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010627:Phenethylamines / Q000032:analysis; D014363:Tryptamines / Q000032:analysis; D014439:Tyramine / Q000032:analysis; D014920:Wine / Q000032:analysis*
chemical_list: D001555:Benzene Derivatives; D001679:Biogenic Amines; D010627:Phenethylamines; D014363:Tryptamines; C029261:phenethylamine; C030820:tryptamine; D006632:Histamine; D014439:Tyramine
keywords: 2-Chloro-1,3-dinitro-5-(trifluoromethyl)benzene; Biogenic amines; High-performance liquid chromatography; Spectral and crystallographic analysis; Wine samples