title: Microsatellite instability analysis in uterine cavity washings to detect endometrial cancer in Lynch syndrome.
abstract: OBJECTIVE
  To assess the feasibility of Microsatellite Instability (MSI) analysis in uterine cavity washings for detecting endometrial cancer in Lynch syndrome.
  
  
  METHODS
  This was a proof-of-concept study in Lynch syndrome patients, scheduled for hysterectomy. At the beginning of surgical procedure, uterine cavity washings were performed, and sent for MSI analysis. Pathological examination of the uterus was associated with mismatch repair protein expression and MSI analysis.
  
  
  RESULTS
  Nine patients were included in the study. Uterine cavity washings were feasible and interpretable in all cases. Final histological report identified 2 endometrial cancers and 7 benign specimens. There was no atypical hyperplasia. Sensitivity, specificity, positive predictive value, and negative predictive value of MSI analysis in uterine washings reached 100% in all cases. Concordance of MSI presence or absence was absolute between uterine washings and final histology.
  
  
  CONCLUSIONS
  MSI analysis in uterine cavity washings may be a promising screening tool for Lynch syndrome-associated endometrial cancer diagnosis.
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Chirurgie Cancérologique Gynécologique et du Sein, Paris, France INSERM UMR-S 747, Université Paris Descartes, Sorbonne Paris Cité, Paris, France anne-sophie.bats@egp.aphp.fr.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Bats
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Biochimie, Paris, France INSERM UMR S775, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Helene
authors.initials: H
authors.lastname: Blons
authors.affiliation: Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Biochimie, Paris, France.
authors.forename: Celine
authors.initials: C
authors.lastname: Narjoz
authors.affiliation: INSERM UMR-S 747, Université Paris Descartes, Sorbonne Paris Cité, Paris, France Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Anatomo-pathologie, Paris, France.
authors.forename: Marie-Aude
authors.initials: MA
authors.lastname: Le Frere-Belda
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Biochimie, Paris, France INSERM UMR S775, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Laurent-Puig
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Chirurgie Cancérologique Gynécologique et du Sein, Paris, France INSERM UMR-S 747, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Lecuru
mesh_terms: D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000150:complications / Q000473:pathology / Q000601:surgery*; D016889:Endometrial Neoplasms / Q000175:diagnosis* / Q000209:etiology; D005240:Feasibility Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007044:Hysterectomy / Q000009:adverse effects*; D053842:Microsatellite Instability; D008875:Middle Aged; D011379:Prognosis; D007507:Therapeutic Irrigation; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000209:etiology; D014599:Uterus / Q000473:pathology
keywords: Endometrial cancer; Lynch syndrome; microsatellite instability analysis; screening; uterine cavity washings