title: Sciatic nerve regeneration induced by transplantation of in vitro bone marrow stromal cells into an inside-out artery graft in rat.
abstract: Traumatic injury to peripheral nerves results in considerable motor and sensory disability. Several research groups have tried to improve the regeneration of traumatized nerves by invention of favorable microsurgery. Effect of undifferentiated bone marrow stromal cells (BMSCs) combined with artery graft on peripheral nerve regeneration was studied using a rat sciatic nerve regeneration model. A 10-mm sciatic nerve defect was bridged using an artery graft (IOAG) filled with undifferentiated BMSCs (2 × 10(7) cells/mL). In control group, the graft was filled with phosphated buffer saline alone. The regenerated fibers were studied 4, 8 and 12 weeks after surgery. Assessment of nerve regeneration was based on behavioral, functional (Walking Track Analysis), electrophysiological, histomorphometric and immuohistochemical (Schwann cell detection by S-100 expression) criteria. The behavioral, functional and electrophysiological studies confirmed significant recovery of regenerated axons in IOAG/BMSC group (P < 0.05). Quantitative morphometric analyses of regenerated fibers showed the number and diameter of myelinated fibers in IOAG/BMSC group were significantly higher than in the control group (P < 0.05). This demonstrates the potential of using undifferentiated BMSCs combined with artery graft in peripheral nerve regeneration without limitations of donor-site morbidity associated with isolation of Schwann cells. It is also cost saving due to reduction in interval from tissue collection until cell injection, simplicity of laboratory procedures compared to differentiated BMSCs and may have clinical implications for the surgical management of patients after facial nerve transection.
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, Urmia University, Nazloo Road, Urmia 57153 1177, Iran. Electronic address: r.mohammadi@urmia.ac.ir.
authors.forename: Rahim
authors.initials: R
authors.lastname: Mohammadi
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Salmas Road, Urmia 3737, Iran.
authors.forename: Behnam
authors.initials: B
authors.lastname: Vahabzadeh
authors.affiliation: Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada.
authors.forename: Keyvan
authors.initials: K
authors.lastname: Amini
mesh_terms: D000818:Animals; D001158:Arteries; D001369:Axons / Q000473:pathology; D018929:Cell Culture Techniques; D004558:Electric Stimulation; D004576:Electromyography / Q000379:methods; D008124:Locomotion / Q000502:physiology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D018482:Muscle, Skeletal / Q000473:pathology; D009412:Nerve Fibers / Q000473:pathology; D009413:Nerve Fibers, Myelinated / Q000473:pathology; D009416:Nerve Regeneration / Q000502:physiology*; D009431:Neural Conduction / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000502:physiology; D009418:S100 Proteins / Q000032:analysis; D012583:Schwann Cells / Q000473:pathology; D012584:Sciatic Nerve / Q000293:injuries* / Q000601:surgery; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D009418:S100 Proteins
keywords: Artery graft; BMSCs; Peripheral nerve; Regeneration; Sciatic; Undifferentiated