title: Efficacy ofex vivoactivated and expanded natural killer cells and T lymphocytes for colorectal cancer patients.
abstract: Immune cell-based therapies using natural killer (NK) cells and cytotoxic T cells are under constant scrutiny, with the aim to design an effective and reduced-toxicity therapy, which will benefit patients via improved quality of life and improved prognosis. Four patients with stage IV colon cancer were administered 1, 3, 5 and 6 effector cell intravenous infusions, respectively. Peripheral blood was collected from the patients and theex vivoactivation and expansion of NK and T cells was performed in Good Manufacturing Practice-certified clean rooms for ~12-15 days. Immunophenotypic analysis of the peripheral blood mononuclear cells (PBMCs) and expanded NK and T cells was conducted using flow cytometry and the patients were followed up. On average, 4.8×107initial PBMCs and 2.7×109total expanded cells were obtained. The intravenous infusions of the expanded cells were not accompanied by adverse reactions. Improved prognosis, reflected by a considerable decrease in the cancer markers, accompanied by an improved quality of life in the patients were observed. In conclusion, potential strategies are currently under development for the large-scale production of effectors cells; therefore, autologous immune enhancement therapy (AIET) may be considered as a viable approach to cancer treatment.
authors.affiliation: Nichi-Asia Life Science, Sdn. Bhd., Petaling Jaya 47810, Malaysia.
authors.forename: Baskar
authors.initials: B
authors.lastname: Subramani
authors.affiliation: Nichi-Asia Life Science, Sdn. Bhd., Petaling Jaya 47810, Malaysia.
authors.forename: Chithra Ramanathan
authors.initials: CR
authors.lastname: Pullai
authors.affiliation: Nichi-Asia Life Science, Sdn. Bhd., Petaling Jaya 47810, Malaysia.
authors.forename: Kohila
authors.initials: K
authors.lastname: Krishnan
authors.affiliation: Nichi-Asia Life Science, Sdn. Bhd., Petaling Jaya 47810, Malaysia.
authors.forename: Sheela Devi
authors.initials: SD
authors.lastname: Sugadan
authors.affiliation: Biotherapy Institute of Japan, Tokyo 135-0051, Japan.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Deng
authors.affiliation: Nilai Cancer Institute (NCI) Hospital, Nilai 71800, Malaysia.
authors.forename: Terunuma
authors.initials: T
authors.lastname: Hiroshi
authors.affiliation: Nilai Cancer Institute (NCI) Hospital, Nilai 71800, Malaysia.
authors.forename: Kananathan
authors.initials: K
authors.lastname: Ratnavelu
keywords: FOLFOX; autologous immune enhancement therapy; colorectal cancer