title: Hydrogen sulfide treatment induces angiogenesis after cerebral ischemia.
abstract: Hydrogen sulfide (H2 S) is a potent vasodilator and regulates cardiovascular homeostasis. Furthermore, H2 S has a crucial role in ischemia-reperfusion injuries, especially of the heart, liver, and kidneys. This study indicates that treatment with hydrogen sulfide is able to restore neurological function after ischemic stroke by promoting angiogenesis. Treatment with H2 S augments angiogenesis in the peri-infarct area, and it significantly improves functional outcomes after 2 weeks in a rat MCAO model. H2 S promotes the phosphorylation of AKT and ERK and increases the expression of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1). H2 S-treated rats showed more newly synthesized endothelial cells in the ischemic lesion (2.31-fold, P < 0.01). H2 S-treated astrocytes increased VEGF and Ang-1 expression, and the inhibition of phosphatidylinositide 3-kinase (PI3K)/AKT signaling by LY294002 significantly reduced H2 S-induced VEGF and Ang-1 expression in astrocytes. Finally, H2 S stimulated endothelial cell migration (3.92-fold increase in wound healing assay) and tube formation (3.69-fold increase, P < 0.001) through PI3K/AKT signaling. In conclusion, treatment with H2 S promotes angiogenesis and thereby contributes to improvement of functional outcome after cerebral ischemia. Our findings strongly suggest that H2 S may be of value in regenerative recovery after stroke.
authors.affiliation: Neuroscience Research Institute in SNUMRC, Seoul National University, Seoul, Republic of Korea; Department of Neurology, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Hyunduk
authors.initials: H
authors.lastname: Jang
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Oh
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Kim
authors.forename: In-Young
authors.initials: IY
authors.lastname: Choi
authors.forename: Hye Sung
authors.initials: HS
authors.lastname: Yang
authors.forename: Wi-Sun
authors.initials: WS
authors.lastname: Ryu
authors.forename: Seung-Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Byung-Woo
authors.initials: BW
authors.lastname: Yoon
mesh_terms: D000704:Analysis of Variance; D020533:Angiogenesis Inhibitors / Q000627:therapeutic use*; D000818:Animals; D020520:Brain Infarction / Q000188:drug therapy / Q000209:etiology; D002545:Brain Ischemia / Q000150:complications*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000172:deficiency; D006862:Hydrogen Sulfide / Q000627:therapeutic use*; D000860:Hypoxia; D020287:In Situ Nick-End Labeling; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000209:etiology*; D009422:Nervous System Diseases / Q000188:drug therapy / Q000209:etiology; D051058:Oncogene Protein v-akt / Q000378:metabolism; D051381:Rats; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D042461:Vascular Endothelial Growth Factor A; D051058:Oncogene Protein v-akt; D005947:Glucose; D006862:Hydrogen Sulfide
keywords: acute stroke; angiogenesis; animal models; cell culture; cerebrovascular disease