title: Expression, purification, and immobilization of recombinant tamavidin 2 fusion proteins.
abstract: Tamavidin 2 is a fungal avidin-like protein that binds biotin with high affinity. Unlike avidin or streptavidin, tamavidin 2 in soluble form is produced at high levels in Escherichia coli. In this chapter, we describe a method for immobilization and purification of recombinant proteins with the use of tamavidin 2 as an affinity tag. The protein fused to tamavidin 2 is tightly immobilized and simultaneously purified on biotinylated magnetic microbeads without loss of activity.
authors.affiliation: Plant Innovation Center, Japan Tobacco, Inc., 700 Higashibara, Iwata, Shizuoka, 438-0802, Japan, yoshimitsu.takakura@jt.com.
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Takakura
authors.forename: Naomi
authors.initials: N
authors.lastname: Oka
authors.forename: Masako
authors.initials: M
authors.lastname: Tsunashima
mesh_terms: D001360:Avidin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D001710:Biotin / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D055767:Immobilized Proteins / Q000737:chemistry; D008967:Molecular Biology / Q000379:methods*; D020076:Pleurotus / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D019809:Streptavidin / Q000737:chemistry
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; D055767:Immobilized Proteins; D011993:Recombinant Fusion Proteins; C550582:tamavidin 2, Pleurotus cornucopiae; D001360:Avidin; D001710:Biotin; D019809:Streptavidin