title: MicroRNA-196a/-196b promote cell metastasis via negative regulation of radixin in human gastric cancer.
abstract: MicroRNAs (miRNAs) play an important role to contribute carcinogenesis. The aim of the current study was to identify useful biomarkers from miRNAs. Differential miRNA profiles were analyzed using the miRNA qRT-PCR-based assay. Two of the most upregulated miRNAs were selected and validated. The miR-196a/-196b levels were significantly increased in gastric cancer (GC) tissues (n=109). Overexpression of miR-196a/-196b was significantly associated with tumor progression and poorer 5-year survival outcomes. Overexpression of miR-196a/-196b enhances GC cell migration and invasion. Further, radixin was identified as a target gene of miR-196a/-196b. Elevated miR-196a/-196b expression in GC cells led to reduced radixin protein levels and vice versa. Notably, an inverse correlation between miR-196a/-196b and radixin mRNA and protein expression was observed in GC tissues with in situ hybridization and immunohistochemistry analyses. Together, miR-196a/-196b inhibitory oligonucleotides or overexpression of the radixin may thus have therapeutic potential in suppressing GC metastasis.
authors.affiliation: Department of Nursing, Chang-Gung University of Science and Technology, Taoyuan 333, Taiwan.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Tsai
authors.affiliation: Department of General Surgery, Chang Gung Memorial Hospital at Chiayi, Chiayi 613, Taiwan.
authors.forename: Chia-Siu
authors.initials: CS
authors.lastname: Wang
authors.affiliation: Department of Biochemistry, College of Medicine, Chang-Gung University, Taoyuan 333, Taiwan.
authors.forename: Chung-Ying
authors.initials: CY
authors.lastname: Tsai
authors.affiliation: Department of Biochemistry, College of Medicine, Chang-Gung University, Taoyuan 333, Taiwan; Department of Medical Research, Mackay Memorial Hospital, Taipei 251, Taiwan.
authors.forename: Cheng-Yi
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Biochemistry, College of Medicine, Chang-Gung University, Taoyuan 333, Taiwan.
authors.forename: Hsiang-Cheng
authors.initials: HC
authors.lastname: Chi
authors.affiliation: Department of Biochemistry, College of Medicine, Chang-Gung University, Taoyuan 333, Taiwan.
authors.forename: Yi-Hsin
authors.initials: YH
authors.lastname: Tseng
authors.affiliation: Molecular Medicine Research Center, College of Medicine, Chang-Gung University, Taoyuan 333, Taiwan.
authors.forename: Pei-Jung
authors.initials: PJ
authors.lastname: Chung
authors.affiliation: Department of Biochemistry, College of Medicine, Chang-Gung University, Taoyuan 333, Taiwan.
authors.forename: Yang-Hsiang
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Department of Biochemistry, College of Medicine, Chang-Gung University, Taoyuan 333, Taiwan.
authors.forename: I-Hsiao
authors.initials: IH
authors.lastname: Chung
authors.affiliation: Department of Biochemistry, College of Medicine, Chang-Gung University, Taoyuan 333, Taiwan.
authors.forename: Ching-Ying
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Biochemistry, College of Medicine, Chang-Gung University, Taoyuan 333, Taiwan. Electronic address: khlin@mail.cgu.edu.tw.
authors.forename: Kwang-Huei
authors.initials: KH
authors.lastname: Lin
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: D003598:Cytoskeletal Proteins; C542543:MIRN196 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; C071776:radixin
keywords: Gastric cancer; Metastasis; Prognosis; Radixin; miR-196a/-196b