title: High-throughput production of two disulphide-bridge toxins.
abstract: A quick and efficient production method compatible with high-throughput screening was developed using 36 toxins belonging to four different families of two disulphide-bridge toxins. Final toxins were characterized using HPLC co-elution, CD and pharmacological studies.
authors.affiliation: CEA, DSV, iBiTec-S, Service d'Ingénierie Moléculaire des Protéines (SIMOPRO), CEA Saclay, Gif sur Yvette F-91191, France. gregory.upert@cea.fr nicolas.gilles@cea.fr.
authors.forename: Grégory
authors.initials: G
authors.lastname: Upert
authors.forename: Gilles
authors.initials: G
authors.lastname: Mourier
authors.forename: Alexandra
authors.initials: A
authors.lastname: Pastor
authors.forename: Marion
authors.initials: M
authors.lastname: Verdenaud
authors.forename: Doria
authors.initials: D
authors.lastname: Alili
authors.forename: Denis
authors.initials: D
authors.lastname: Servent
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gilles
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004220:Disulfides / Q000737:chemistry*; D008969:Molecular Sequence Data; D014118:Toxins, Biological / Q000737:chemistry / Q000145:classification / Q000378:metabolism*
chemical_list: D004220:Disulfides; D014118:Toxins, Biological