title: Influence of different probiotic lactic Acid bacteria on microbiota and metabolism of rats with dysbiosis.
abstract: Lactic acid bacteria (LAB) are often used for prevention and treatment of dysbiosis. However, the action of various strains of LAB on metabolism and digestion under these conditions are poorly understood. The purpose of this study was to investigate the influence of probiotic LAB on metabolism, digestion and microbiota in animals with dysbiosis. After administration of ampicillin and metronidazole male Wistar rats, were fed products containing Enterococcus faecium L3 (E.f.), Lactobacillus fermentum Z (L.f.) or milk (control 1). Animals in control group 2 were fed milk, after water instead of antibiotics. Dyspeptic symptoms disappeared after administration of probiotic compared with control 1. At the end of the experiment, an increase in the content of enterococci and lactobacilli in the proximal part of the small intestine was found in the animals treated with E.f. and L.f., respectively. After the introduction of probiotic enterococci, the quantity of lactobacilli and bifidobacteria in the intestines of rats increased, and the content of Klebsiella spp. and Escherichia coli decreased in comparison with the control group 1 and the group fed lactobacilli. The activity of alkaline phosphatase and aspartate transaminase was greater in blood serum of rats with dysbiosis receiving milk and lactobacilli. Intestinal alkaline phosphatase activity increased in the epithelium and chyme in the jejunum of the animals treated with L. f. and in the chyme only in the animals treated with E. f. Thus, the specific effects of different strains of probiotic LAB on the microbiota, and on metabolism and digestion of various nutrients were demonstrated.
authors.affiliation: Department of Molecular Microbiology, Institute of Experimental Medicine, acad. Pavlov str. 12, Saint Petersburg 197376, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Ermolenko
authors.affiliation: Department of Physiology of Nutrition, Pavlov Institute of Physiology, Makarova emb.6, Saint Petersburg 199034, Russia.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Gromova
authors.affiliation: Department of Physiology of Nutrition, Pavlov Institute of Physiology, Makarova emb.6, Saint Petersburg 199034, Russia.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Borschev
authors.affiliation: Department of Molecular Microbiology, Institute of Experimental Medicine, acad. Pavlov str. 12, Saint Petersburg 197376, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Voeikova
authors.affiliation: Department of Molecular Microbiology, Institute of Experimental Medicine, acad. Pavlov str. 12, Saint Petersburg 197376, Russia.
authors.forename: Alena
authors.initials: A
authors.lastname: Karaseva
authors.affiliation: Laboratory of Immunology, Pasteur Institute of Epidemiology and Microbiology, Mira str. 16, Saint Petersburg 197376, Russia.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Ermolenko
authors.affiliation: Department of Physiology of Nutrition, Pavlov Institute of Physiology, Makarova emb.6, Saint Petersburg 199034, Russia.
authors.forename: Andrei
authors.initials: A
authors.lastname: Gruzdkov
authors.affiliation: Department of Molecular Microbiology, Institute of Experimental Medicine, acad. Pavlov str. 12, Saint Petersburg 197376, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Suvorov
keywords: dysbiosis; enterococci; lactobacilli; metabolism