title: [Effects of botulinum toxin type A on the expression of alpha-SMA and myosin-II of fibroblasts in scars].
abstract: OBJECTIVE
  To investigate the effects of botulinum toxin type A (BTXA) on the expression of alpha smooth muscle actin(alpha-SMA) and myosin-II of fibroblasts in scars. Methods Fibroblasts were isolated from tissue specimens of scars contracture. Cells from passages 3-5 were randomly divided into 3 groups (control group, low BTXA group (1 U/10(6) Cells), and high BTXA group (2.5 U/ 10(6)Cells)). Growth condition of fibroblasts was observed at 1 , 4, 7 day after BTXA treated. Changes of alpha-SMA and myosin-II in fibroblasts were detected by Western blot.
  
  
  RESULTS
  Fibroblasts grew well in control group. The proliferation was decreased 4 days later in BTXA groups. Lots of apoptotic cells were seen in high BTXA group at 7th day. Proteins of alpha-SMA and myosin-II in fibroblasts were statistically different between BTXA group and control groups at 4th day (P < 0.05). The expression of alpha-SMA and myosin-II in low BTXA group was higher than that in high BTXA group at 7th day (P < 0.05).
  
  
  CONCLUSIONS
  BTXA could induce the apoptosis of fibroblasts and decrease the expression of alpha-SMA and myosin-II in fibroblasts. The inhibitory effect was strengthened with BTXA concentration increase within a certain range.
authors.forename: Tongtong
authors.initials: T
authors.lastname: Yan
authors.forename: Minliang
authors.initials: M
authors.lastname: Chen
authors.forename: Kui
authors.initials: K
authors.lastname: Ma
authors.forename: Liming
authors.initials: L
authors.lastname: Liang
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Linying
authors.initials: L
authors.lastname: Lai
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
mesh_terms: D000199:Actins / Q000378:metabolism*; D019274:Botulinum Toxins, Type A / Q000494:pharmacology*; D002921:Cicatrix; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D009130:Muscle, Smooth / Q000378:metabolism*; D024462:Myosin Type II / Q000378:metabolism*; D011897:Random Allocation
chemical_list: D000199:Actins; D019274:Botulinum Toxins, Type A; D024462:Myosin Type II