title: Edaravone protected PC12 cells against MPP(+)-cytoxicity via inhibiting oxidative stress and up-regulating heme oxygenase-1 expression.
abstract: Oxidative stress is involved in the pathogenesis of Parkinson's disease (PD). Edaravone has been shown to have a neuroprotective effect. In the present work, we investigated the effect of edaravone on 1-methyl-4-phenylpyridinium (MPP(+))-treated PC12 cells. Edaravone inhibited the decrease of cell viability and apoptosis induced by MPP(+) in PC12 cells. In addition, edaravone alleviated intracellular reactive oxygen species (ROS) production. MPP(+) induced heme oxygenase-1 (HO-1) expression, which was further enhanced by edaravone. The inhibitor of HO-1 zinc protoporphyrin-IX attenuated the neuroprotection of edaravone. So edaravone protected PC12 cells against MPP(+)-cytoxicity via inhibiting oxidative stress and up-regulating HO-1 expression. The data showed that edaravone was neuroprotective and could be potentially therapeutics for PD in future.
authors.affiliation: Neurobiology Institute, Jining Medical University, Jining 272067, PR China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Cheng
authors.affiliation: Shandong University School of Medicine, Jinan 250012, PR China.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Guo
authors.affiliation: The Second Hospital of Shandong University, Jinan 250033, PR China.
authors.forename: Chuangang
authors.initials: C
authors.lastname: Li
authors.affiliation: Neurobiology Institute, Jining Medical University, Jining 272067, PR China.
authors.forename: Bingyuan
authors.initials: B
authors.lastname: Ji
authors.affiliation: Neurobiology Institute, Jining Medical University, Jining 272067, PR China.
authors.forename: Yanyou
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Neurobiology Institute, Jining Medical University, Jining 272067, PR China. Electronic address: chenjnmc@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Neurobiology Institute, Jining Medical University, Jining 272067, PR China. Electronic address: baibo1@mail.jnmc.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Bai
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D000077553:Edaravone; D004791:Enzyme Inhibitors; D005434:Flow Cytometry; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D009498:Neurotoxins / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells / Q000187:drug effects; D011524:Protoporphyrins / Q000494:pharmacology; D051381:Rats; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D009498:Neurotoxins; D011524:Protoporphyrins; C017803:zinc protoporphyrin; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D000077553:Edaravone; D000983:Antipyrine
keywords: 1-Methyl-4-phenylpyridinium; Edaravone; Heme oxygenase-1; Oxidative stress; PC12; Parkinson's disease