title: Leukotriene enhanced allergic lung inflammation through induction of chemokine production.
abstract: The leukotrienes (LTs) enhance allergen- and interleukin (IL)-13-dependent allergic lung inflammatory disease. However, the precise requirement of LTs and the mechanism by which they elicit allergic lung responses remain uncertain. To clarify the involvement of LTs in respiratory allergen- and IL-13-induced experimental asthma and elucidate the underlying mechanisms of LTs-mediated enhanced allergic asthma, we investigated the role of LTs in two models of allergic inflammation: intranasal Aspergillus protease allergen and recombinant IL-13-induced T helper type 2 (Th2) cell-mediated inflammation, and also examined Th2-related chemokines downstream of LTs signaling. 5-Lipoxygenase (5-LO)-deficient mice exposed to short-term intranasal Aspergillus protease allergen showed attenuated airway inflammation, decreased airway hyper-responsiveness and reduced bronchoalveolar eosinophilia when compared to wild-type mice. However, this phenotype was less apparent using long exposure to the same allergen. 5-LO-deficient mice exposed to intranasal rIL-13 also showed attenuated phenotypes of allergic asthma via significant reduction in Th2-specific chemokines, CCL7 and CCL17 production and decreased Th2 cells recruitment to the lungs. Addition of leukotriene B4 (LTB4) and LTC4 to the airways of 5-LO-deficient mice resulted in the rescue of rIL-13-induced experimental asthma. Furthermore, LTs addition to rIL-13 synergistically enhanced the production of Th2-specific chemokines in the lung and inflammatory responses. Therefore, our findings suggest that LTs complement allergens and their downstream cytokine (e.g., IL-13) induced Th2 inflammation by enhancing the induction of Th2 chemokines.
authors.affiliation: Cellular Immunology Laboratory, Graduate School of Medical Science and Engineering, Biomedical Research Center, KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Korea.
authors.forename: Kihyuk
authors.initials: K
authors.lastname: Shin
authors.forename: Jung Joo
authors.initials: JJ
authors.lastname: Hwang
authors.forename: Bo-In
authors.initials: BI
authors.lastname: Kwon
authors.forename: Farrah
authors.initials: F
authors.lastname: Kheradmand
authors.forename: David B
authors.initials: DB
authors.lastname: Corry
authors.forename: Seung-Hyo
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D001230:Aspergillus / Q000201:enzymology; D001249:Asthma / Q000473:pathology*; D018925:Chemokines / Q000378:metabolism*; D004195:Disease Models, Animal; D018793:Interleukin-13 / Q000276:immunology; D015289:Leukotrienes / Q000378:metabolism*; D051379:Mice; D010447:Peptide Hydrolases / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000485:Allergens; D018925:Chemokines; D018793:Interleukin-13; D015289:Leukotrienes; D011994:Recombinant Proteins; D010447:Peptide Hydrolases