title: The Cellulase KORRIGAN Is Part of the Cellulose Synthase Complex.
abstract: Plant growth and organ formation depend on the oriented deposition of load-bearing cellulose microfibrils in the cell wall. Cellulose is synthesized by a large relative molecular weight cellulose synthase complex (CSC), which comprises at least three distinct cellulose synthases. Cellulose synthesis in plants or bacteria also requires the activity of an endo-1,4-β-d-glucanase, the exact function of which in the synthesis process is not known. Here, we show, to our knowledge for the first time, that a leaky mutation in the Arabidopsis (Arabidopsis thaliana) membrane-bound endo-1,4-β-d-glucanase KORRIGAN1 (KOR1) not only caused reduced CSC movement in the plasma membrane but also a reduced cellulose synthesis inhibitor-induced accumulation of CSCs in intracellular compartments. This suggests a role for KOR1 both in the synthesis of cellulose microfibrils and in the intracellular trafficking of CSCs. Next, we used a multidisciplinary approach, including live cell imaging, gel filtration chromatography analysis, split ubiquitin assays in yeast (Saccharomyces cerevisiae NMY51), and bimolecular fluorescence complementation, to show that, in contrast to previous observations, KOR1 is an integral part of the primary cell wall CSC in the plasma membrane.
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.).
authors.forename: Thomas
authors.initials: T
authors.lastname: Vain
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.).
authors.forename: Elizabeth Faris
authors.initials: EF
authors.lastname: Crowell
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.).
authors.forename: Hélène
authors.initials: H
authors.lastname: Timpano
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.).
authors.forename: Eric
authors.initials: E
authors.lastname: Biot
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.).
authors.forename: Thierry
authors.initials: T
authors.lastname: Desprez
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.).
authors.forename: Nasim
authors.initials: N
authors.lastname: Mansoori
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.).
authors.forename: Luisa M
authors.initials: LM
authors.lastname: Trindade
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.).
authors.forename: Silvère
authors.initials: S
authors.lastname: Pagant
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.).
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Robert
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.).
authors.forename: Herman
authors.initials: H
authors.lastname: Höfte
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.).
authors.forename: Martine
authors.initials: M
authors.lastname: Gonneau
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., S.P., S.R., H.H., M.G., S.V.);AgroParisTech, Institut Jean-Pierre Bourgin, F-78000 Versailles, France (T.V., E.F.C., H.T., E.B., T.D., H.H., M.G., S.V.); andWageningen University and Research Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands (N.M., L.M.T.) samantha.vernhettes@versailles.inra.fr.
authors.forename: Samantha
authors.initials: S
authors.lastname: Vernhettes