title: Bilirubin oxidase-like proteins from Podospora anserina: promising thermostable enzymes for application in transformation of plant biomass.
abstract: Plant biomass degradation by fungi is a critical step for production of biofuels, and laccases are common ligninolytic enzymes envisioned for ligninolysis. Bilirubin oxidases (BODs)-like are related to laccases, but their roles during lignocellulose degradation have not yet been fully investigated. The two BODs of the ascomycete fungus Podospora anserina were characterized by targeted gene deletions. Enzymatic assay revealed that the bod1(Δ) and bod2(Δ) mutants lost partly a thermostable laccase activity. A triple mutant inactivated for bod1, bod2 and mco, a previously investigated multicopper oxidase gene distantly related to laccases, had no thermostable laccase activity. The pattern of fruiting body production in the bod1(Δ) bod2(Δ) double mutant was changed. The bod1(Δ) and bod2(Δ) mutants were reduced in their ability to grow on ligneous and cellulosic materials. Furthermore, bod1(Δ) and bod2(Δ) mutants were defective towards resistance to phenolic substrates and H2 O2 , which may also impact lignocellulose breakdown. Double and triple mutants were more affected than single mutants, evidencing redundancy of function among BODs and mco. Overall, the data show that bod1, bod2 and mco code for non-canonical thermostable laccases that participate in the degradation of lignocellulose. Thanks to their thermal stability, these enzymes may be more promising candidate for biotechnological application than canonical laccases.
authors.affiliation: Sorbonne Paris Cité, Institut des Energies de Demain (IED), Université Paris Diderot, UMR 8236, 75205, Paris, France; Institut de Génétique et Microbiologie, Université Paris Sud, UMR 8621, 91405, Orsay, France.
authors.forename: Ning
authors.initials: N
authors.lastname: Xie
authors.forename: Gwenaël
authors.initials: G
authors.lastname: Ruprich-Robert
authors.forename: Philippe
authors.initials: P
authors.lastname: Silar
authors.forename: Florence
authors.initials: F
authors.lastname: Chapeland-Leclerc
mesh_terms: D000595:Amino Acid Sequence; D056804:Biofuels; D001709:Biotechnology / Q000379:methods; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D042845:Laccase / Q000235:genetics; D008031:Lignin / Q000378:metabolism*; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000235:genetics; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D048468:Podospora / Q000201:enzymology*; D016415:Sequence Alignment; D017384:Sequence Deletion / Q000235:genetics
chemical_list: D056804:Biofuels; D005656:Fungal Proteins; C036909:lignocellulose; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase