title: Effect of resection of localized pancreaticobiliary adenocarcinoma on angiogenic markers and tissue factor related pro-thrombotic and pro-angiogenic activity.
abstract: In this study, 52 patients were studied to elucidate the relative impact of resection of localized pancreaticobiliary adenocarcinoma (PBC) on circulating factors of tumour-associated angiogenesis e.g. tissue factor bearing microparticles (TFMP) and vascular endothelial growth factor (VEGF) and their clinicopathological significance to angiogenesis markers in cancer tissue from PBC patients. Angiogenesis array analysis on serum samples revealed that surgical resection of tumour lesion in PBC patients affects the levels of a panel of angiogenesis-related molecules, including VEGF that was verified by ELISA to significantly reduce (median & IQR: 1003(369-2000) vs. 457(159-834) pg/ml; p<0.05). Correspondingly, a significant decrease in the angiogenic activity (decreased capillary tube formation; p<0.05) of serum samples after the surgery was also found. Despite a decrease in number of circulating TFMP after surgery, this did not reach statistical significance; there was a significant reduction in pro-coagulant activity (prolonged prothrombin time, p<0.001) post-operatively. In addition, the activity of total microparticles (MP activity assay, p<0.05) was decreased significantly. Immunohistochemical staining of tumour tissue revealed a strong correlation between the microvessel density (MVD) and VEGF expression. Also, higher levels of circulating TFMP or TF related activity (prothrombin time) correlated significantly with TF expression and MVD on tumour tissues from PBC patients. These findings suggest that in pancreaticobiliary adenocarcinoma TF related angiogenesis drivers are equally significant to VEGF ones, raising the clinical question of whether the effectiveness of angiogenesis targeting studies could be improved through the 'dual' targeting of these pathways in PBC.
authors.affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull, HU6 7RX, UK.
authors.forename: H H
authors.initials: HH
authors.lastname: Echrish
authors.affiliation: Hull and York Medical School, University of Hull, Hull, HU6 7RX, UK.
authors.forename: Y
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull, HU6 7RX, UK.
authors.forename: L A
authors.initials: LA
authors.lastname: Madden
authors.affiliation: Hull and York Medical School, University of Hull, Hull, HU6 7RX, UK.
authors.forename: V
authors.initials: V
authors.lastname: Allgar
authors.affiliation: Department of Pathology, Hull and East Yorkshire Hospitals NHS Trust, Hull, HU3 2JZ, UK.
authors.forename: J
authors.initials: J
authors.lastname: Cooke
authors.affiliation: Department of Hepatobiliary Surgery, Castle Hill Hospital, Cottingham, HU16 5JQ, UK.
authors.forename: K
authors.initials: K
authors.lastname: Wedgwood
authors.affiliation: Department of Hepatobiliary Surgery, Castle Hill Hospital, Cottingham, HU16 5JQ, UK.
authors.forename: D
authors.initials: D
authors.lastname: Dasgupta
authors.affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull, HU6 7RX, UK.
authors.forename: J
authors.initials: J
authors.lastname: Greenman
authors.affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull, HU6 7RX, UK; Hull and York Medical School, University of Hull, Hull, HU6 7RX, UK; Queen's Centre for Oncology and Haematology, Castle Hill Hospital, Cottingham, HU16 5JQ, UK. Electronic address: anthony.maraveyas@hey.nhs.uk.
authors.forename: A
authors.initials: A
authors.lastname: Maraveyas
mesh_terms: D000230:Adenocarcinoma / Q000097:blood* / Q000150:complications / Q000473:pathology / Q000601:surgery; D000368:Aged; D055252:Cell-Derived Microparticles / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000097:blood* / Q000150:complications; D010179:Pancreas / Q000098:blood supply / Q000473:pathology* / Q000601:surgery; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000150:complications / Q000473:pathology / Q000601:surgery; D013925:Thromboplastin / Q000032:analysis*; D013927:Thrombosis / Q000097:blood* / Q000150:complications; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000097:blood
chemical_list: D042461:Vascular Endothelial Growth Factor A; D013925:Thromboplastin
keywords: Angiogenesis; Microparticle; Pancreaticobiliary adenocarcinoma; Thrombosis; Tissue factor