title: Syngeneic transplantation of newborn splenocytes in a murine model of neonatal ischemia-reperfusion brain injury.
abstract: OBJECTIVE
  Neonatal hypoxic-ischemic encephalopathy (HIE) is caused by brain injury that occurs in a developing fetus or infant. Stem cell transplantation can reportedly induce functional recovery in animal models of HIE. Murine neonatal splenocytes are enriched with immature blood stem cells and are used for the investigation of murine models of syngeneic transplantation. The aim of this study was to investigate the therapeutic potential of newborn splenocytes in a murine model of neonatal ischemia-reperfusion brain injury.
  
  
  METHODS
  C57BL/6N mice (postnatal day 7) underwent right common carotid artery occlusion with an aneurysm clip. Following hypoxic exposure, reperfusion was achieved by unclamping the artery. Newborn splenocytes were transplanted intravenously at 3 weeks after injury.
  
  
  RESULTS
  The splenocytes transplanted group tended to show an improvement in behavioral tests, but it was not significantly different compared with the control groups. The transplanted cells were localized in various organs including injured brain tissue over 3 weeks. In the penumbra region of the brain, vascular endothelial growth factor (VEGF) expression was upregulated after transplantation.
  
  
  CONCLUSIONS
  These results showed that syngeneic transplantation of newborn splenocytes achieved the long-term survival of the grafts and exerted influence the microenvironment in the injured brains of mice.
authors.affiliation: Center for Innovative and Translational Medicine, Kochi University Medical School , Nankoku, Kochi , Japan .
authors.forename: Feifei
authors.initials: F
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Shen
authors.forename: Emi
authors.initials: E
authors.lastname: Tsuru
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Yamashita
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Baba
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tsuda
authors.forename: Nagamasa
authors.initials: N
authors.lastname: Maeda
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sagara
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005434:Flow Cytometry; D020925:Hypoxia-Ischemia, Brain / Q000175:diagnosis / Q000628:therapy*; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging; D051379:Mice; D008810:Mice, Inbred C57BL; D015427:Reperfusion Injury / Q000175:diagnosis / Q000628:therapy*; D013154:Spleen / Q000166:cytology*; D033581:Stem Cell Transplantation; D014185:Transplantation, Isogeneic; D016896:Treatment Outcome
keywords: Cerebral palsy; stem cells; umbilical cord blood