title: Gene expression in B-1 cells from lupus-prone mice.
abstract: New Zealand Black X New Zealand White F1 [(NZB/NZW)F1] mice develop an autoimmune condition with similarities to human systemic lupus erythematosus (SLE). In this study, we demonstrate that B-1 cells, which have previously been reported to be involved in several autoimmune diseases, have altered gene expression in these mice. RNA was extracted from purified B-1 cells of disease-free C57BL/6 mice and lupus-prone (NZB/NZW)F1 mice. Gene expression was analysed using DNA microarray techniques and validated by real time reverse transcriptase polymerase chain reaction (RT-PCR). In (NZB/NZW)F1 mice, some genes had altered expression patterns compared to disease-free controls. Specifically, the upregulation of Ifitm1, Pvrl2 and Ifi202b and downregulation of Trp53bp1 mRNA were observed in (NZB/NZW)F1 mice. These genes are known to be associated with autoimmune diseases. This pattern of gene expression in B-1 cells could understanding of the pathogenesis of SLE. Thus, it is reasonable to hypothesise that the altered gene expression observed in B-1 cells in our experimental model is important for SLE prognosis and therapy, and these implications are discussed herein.
authors.affiliation: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia Universidade Federal de São Paulo , Brazil .
authors.forename: Ronni Rômulo
authors.initials: RR
authors.lastname: Novaes E Brito
authors.forename: Patricia
authors.initials: P
authors.lastname: Xander
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Pérez
authors.forename: Juliana T
authors.initials: JT
authors.lastname: Maricato
authors.forename: Maria Fl
authors.initials: MF
authors.lastname: Laurindo
authors.forename: Beatriz H P
authors.initials: BH
authors.lastname: De Lorenzo
authors.forename: Renata
authors.initials: R
authors.lastname: Pellegrino
authors.forename: Viviane
authors.initials: V
authors.lastname: Bernardo
authors.forename: José Daniel
authors.initials: JD
authors.lastname: Lopes
authors.forename: Mario
authors.initials: M
authors.lastname: Mariano
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis
keywords: (NZB/NZW)F1; Autoimmunity; B-1 cells; SLE; microarray