title: Manganese accumulation in bone following chronic exposure in rats: steady-state concentration and half-life in bone.
abstract: Literature data indicate that bone is a major storage organ for manganese (Mn), accounting for 43% of total body Mn. However, the kinetic nature of Mn in bone, especially the half-life (t(1/2)), remained unknown. This study was designed to understand the time-dependence of Mn distribution in rat bone after chronic oral exposure. Adult male rats received 50 mg Mn/kg (as MnCl2) by oral gavage, 5 days per week, for up to 10 weeks. Animals were sacrificed every 2 weeks during Mn administration for the uptake study, and on day 1, week 2, 4, 8, or 12 after the cessation at 6-week Mn exposure for the t(1/2) study. Mn concentrations in bone (MnBn) were determined by AAS analysis. By the end of 6-week's treatment, MnBn appeared to reach the steady state (T(ss)) level, about 2-3.2 fold higher than MnBn at day 0. Kinetic calculation revealed t(1/2)s of Mn in femur, tibia, and humerus bone of 77 (r=0.978), 263 (r=0.988), and 429 (r=0.994) days, respectively; the average t(1/2) in rat skeleton was about 143 days, equivalent to 8.5 years in human bone. Moreover, MnBn were correlated with Mn levels in striatum, hippocampus, and CSF. These data support MnBn to be a useful biomarker of Mn exposure.
authors.affiliation: School of Health Sciences, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Stefanie L
authors.initials: SL
authors.lastname: O'Neal
authors.affiliation: School of Health Sciences, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Lan
authors.initials: L
authors.lastname: Hong
authors.affiliation: School of Health Sciences, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Sherleen
authors.initials: S
authors.lastname: Fu
authors.affiliation: School of Health Sciences, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Wendy
authors.initials: W
authors.lastname: Jiang
authors.affiliation: School of Health Sciences, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Jones
authors.affiliation: School of Health Sciences, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Linda H
authors.initials: LH
authors.lastname: Nie
authors.affiliation: School of Health Sciences, Purdue University, West Lafayette, IN 47907, USA. Electronic address: wzheng@purdue.edu.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000465:Algorithms; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001842:Bone and Bones / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism; D006207:Half-Life; D007700:Kinetics; D015636:Magnesium Chloride / Q000378:metabolism / Q000493:pharmacokinetics; D008297:Male; D008345:Manganese / Q000378:metabolism* / Q000493:pharmacokinetics; D008670:Metals / Q000737:chemistry / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010599:Pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013054:Spectrophotometry, Atomic; D014018:Tissue Distribution
chemical_list: D008670:Metals; D015636:Magnesium Chloride; D008345:Manganese
keywords: Biomarker; Bone; Disposition; Half-life; Manganese