title: Repression of chimeric transcripts emanating from endogenous retrotransposons by a sequence-specific transcription factor.
abstract: BACKGROUND
  Retroviral elements are pervasively transcribed and dynamically regulated during development. While multiple histone- and DNA-modifying enzymes have broadly been associated with their global silencing, little is known about how the many diverse retroviral families are each selectively recognized.
  
  
  RESULTS
  Here we show that the zinc finger protein Krüppel-like Factor 3 (KLF3) specifically silences transcription from the ORR1A0 long terminal repeat in murine fetal and adult erythroid cells. In the absence of KLF3, we detect widespread transcription from ORR1A0 elements driven by the master erythroid regulator KLF1. In several instances these aberrant transcripts are spliced to downstream genic exons. One such chimeric transcript produces a novel, dominant negative isoform of PU.1 that can induce erythroid differentiation.
  
  
  CONCLUSIONS
  We propose that KLF3 ensures the integrity of the murine erythroid transcriptome through the selective repression of a particular retroelement and is likely one of multiple sequence-specific factors that cooperate to achieve global silencing.
authors.forename: Ka Sin
authors.initials: KS
authors.lastname: Mak
authors.forename: Jon
authors.initials: J
authors.lastname: Burdach
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Norton
authors.forename: Richard C M
authors.initials: RC
authors.lastname: Pearson
authors.forename: Merlin
authors.initials: M
authors.lastname: Crossley
authors.forename: Alister P W
authors.initials: AP
authors.lastname: Funnell
mesh_terms: D000818:Animals; D002460:Cell Line; D041905:Erythroid Cells / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018626:Retroelements / Q000235:genetics*; D020079:Terminal Repeat Sequences / Q000235:genetics*; D059467:Transcriptome
chemical_list: C529780:Klf3 protein, mouse; D051741:Kruppel-Like Transcription Factors; D012333:RNA, Messenger; D018626:Retroelements; C080774:erythroid Kruppel-like factor