title: Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells.
abstract: We created an Nse-CreERT2 mouse line expressing the tamoxifen-inducible CreERT2 recombinase under the control of the neuron-specific enolase (Nse) promoter. By using Cre reporter lines we could show that this Nse-CreERT2 line has recombination activity in the granule cells of all cerebellar lobules as well as in postmitotic granule cell precursors in the external granular layer of the developing cerebellum. A few hippocampal dentate gyrus granule cells showed Cre-mediated recombination as well. Cre activity could be induced in both the developing and adult mouse brain. The established mouse line constitutes a valuable tool to study the function of genes expressed by cerebellar granule cells in the developing and adult brain. In combination with reporter lines it is a useful model to analyze the development and maintenance of the cerebellar architecture including granule cell distribution, migration, and the extension of granule cell fibers in vivo.
authors.affiliation: Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, Freiburg, Germany; Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.
authors.forename: Theresa
authors.initials: T
authors.lastname: Pohlkamp
authors.affiliation: Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, Freiburg, Germany.
authors.forename: Laura
authors.initials: L
authors.lastname: Steller
authors.affiliation: Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, Freiburg, Germany; Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University, Düsseldorf, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: May
authors.affiliation: Department of Urology, University Hospital Freiburg, Freiburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Günther
authors.affiliation: Department of Urology, University Hospital Freiburg, Freiburg, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Schüle
authors.affiliation: Institute for Structural Neurobiology, Center for Molecular Neurobiology, Hamburg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Frotscher
authors.affiliation: Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, Freiburg, Germany; Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.
authors.forename: Joachim
authors.initials: J
authors.lastname: Herz
authors.affiliation: Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, Freiburg, Germany; Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University, Düsseldorf, Germany.
authors.forename: Hans H
authors.initials: HH
authors.lastname: Bock
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000166:cytology*; D005818:Genetic Engineering / Q000379:methods*; D019426:Integrases / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: C045073:Cre recombinase; D019426:Integrases; D010751:Phosphopyruvate Hydratase