title: The large GTPase Mx1 is involved in apical transport in MDCK cells.
abstract: In epithelial cells apical proteins are transported by specific transport carriers to the correct membrane domain. The composition of these carriers is heterogeneous and comprises components such as motor proteins, annexins, lectins, Rab GTPases and cargo molecules. Here, we provide biochemical and fluorescence microscopic data to show that the dynamin-related large GTPase Mx1 is a component of post-Golgi vesicles carrying the neurotrophin receptor p75(NTR) . Moreover, siRNA-mediated depletion of Mx1 significantly decreased the transport efficiency of apical proteins in MDCK cells. In conclusion, Mx1 plays a crucial role in the delivery of cargo molecules to the apical membrane of epithelial cells.
authors.affiliation: Department of Cell Biology and Cell Pathology, Philipps University of Marburg, Robert-Koch-Str. 6, 35037, Marburg, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Hoff
authors.forename: Christoph
authors.initials: C
authors.lastname: Greb
authors.forename: Christina
authors.initials: C
authors.lastname: Hollmann
authors.forename: Ellena
authors.initials: E
authors.lastname: Hönig
authors.forename: Ralf
authors.initials: R
authors.lastname: Jacob
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D008856:Microscopy, Fluorescence / Q000379:methods; D021381:Protein Transport / Q000502:physiology*; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D020800:Receptor, Nerve Growth Factor; D020558:GTP Phosphohydrolases
keywords: MDCK cells; Mx proteins; apical trafficking; polarized epithelia; transport vesicle