title: EMMPRIN reduction via scFv-M6-1B9 intrabody affects α3β1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2.
abstract: Extracellular matrix metalloproteinase inducer (EMMPRIN) exhibits overexpression in various cancers and promotes cancer progression and metastasis via the interaction with its associated molecules. The scFv-M6-1B9 intrabody has a potential ability to reduce EMMPRIN cell surface expression. However, the subsequent effect of scFv-M6-1B9 intrabody-mediated EMMPRIN abatement on its related molecules, α3β1-integrin, MCT1, MMP-2 and MMP-9, is undefined. Our results demonstrated that the scFv-M6-1B9 intrabody efficiently decreased α3β1-integrin cell surface expression levels. In addition, intracellular accumulation of MCT1 and lactate were increased. These results lead to suppression of features characteristic for tumor progression, including cell migration, proliferation and invasion, in a colorectal cancer cell line (Caco-2) although there was no difference in MMP expression. Thus, EMMPRIN represents an attractive target molecule for the disruption of cancer proliferation and metastasis. An scFv-M6-1B9 intrabody-based approach could be relevant for cancer gene therapy.
authors.affiliation: Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Sangboonruang
authors.affiliation: Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Thammasit
authors.affiliation: Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Intasai
authors.affiliation: 1] Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand [2] Biomedical Technology Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: W
authors.initials: W
authors.lastname: Kasinrerk
authors.affiliation: 1] Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand [2] Biomedical Technology Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand [3] BioMedical Engineering Center, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: C
authors.initials: C
authors.lastname: Tayapiwatana
authors.affiliation: Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: K
authors.initials: K
authors.lastname: Tragoolpua
mesh_terms: D000918:Antibody Specificity; D051926:Basigin / Q000276:immunology* / Q000378:metabolism*; D018938:Caco-2 Cells / Q000187:drug effects* / Q000473:pathology; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000473:pathology; D006801:Humans; D039201:Integrin alpha3beta1 / Q000276:immunology / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D027501:Monocarboxylic Acid Transporters / Q000276:immunology / Q000378:metabolism*; D057127:Single-Chain Antibodies / Q000235:genetics / Q000378:metabolism*; D027981:Symporters / Q000276:immunology / Q000378:metabolism*; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D051926:Basigin; D039201:Integrin alpha3beta1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D027501:Monocarboxylic Acid Transporters; D057127:Single-Chain Antibodies; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1; C497886:BSG protein, human