title: A heterogeneous biotin-streptavidin-amplified enzyme-linked immunosorbent assay for detecting tris(2,3-dibromopropyl) isocyanurate in natural samples.
abstract: Tris(2,3-dibromopropyl) isocyanurate (TBC) is a novel brominated flame retardant (BFR) that is widely used to substitute the prohibited BFRs throughout the world. With the development of research, the potential environmental and ecological harms of TBC have been revealed. For sensitive and selective detecting TBC, an indirect competitive biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) has been established in this study. The small molecular TBC-hapten was synthesized first; it mimicked the chemical structure of TBC and possessed a secondary amine group. The as-obtained hapten was then conjugated with carrier proteins to prepare artificial antigen. After immunization, the anti-TBC polyclonal antibody was obtained from separating rabbit serum. The procedures of this BA-ELISA were optimized. Under the optimal conditions, the limit of detection (IC10) was 0.0067 ng/ml and the median inhibitory concentration (IC50) was 0.66 ng/ml. Cross-reactivity values of the BA-ELISA with the tested TBC analogues were ⩽5%. This immunoassay was successfully applied to determine the TBC residue in river water samples that were collected near a BFR manufacturing plant. Satisfactory recoveries (92.1-109.2%) were obtained. The results indicated that this proposed BA-ELISA is suitable for the rapid and sensitive determining of TBC in environmental monitoring.
authors.affiliation: School of Environment Science and Technology, Shanghai Jiao Tong University, Minghang District, Shanghai 200240, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Bu
authors.affiliation: School of Environment Science and Technology, Shanghai Jiao Tong University, Minghang District, Shanghai 200240, People's Republic of China. Electronic address: huishengzhuang@126.com.
authors.forename: Huisheng
authors.initials: H
authors.lastname: Zhuang
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Minghang District, Shanghai 201101, People's Republic of China.
authors.forename: Xinchu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Environment Science and Technology, Shanghai Jiao Tong University, Minghang District, Shanghai 200240, People's Republic of China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D001710:Biotin / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D057230:Limit of Detection; D019809:Streptavidin / Q000378:metabolism*; D014227:Triazines / Q000032:analysis*; D014867:Water / Q000737:chemistry
chemical_list: D000906:Antibodies; D000941:Antigens; D004785:Environmental Pollutants; D014227:Triazines; D014867:Water; C541743:tris(2,3-dibromopropyl)isocyanurate; D001710:Biotin; D019809:Streptavidin
keywords: Biotin–streptavidin-amplified system; Environmental monitoring; Enzyme-linked immunosorbent assay; Tris(2,3-dibromopropyl) isocyanurate