title: Immobilization of hyaluronic acid on plasma-sprayed porous titanium coatings for improving biological properties.
abstract: In the present study, hyaluronic acid (HyA) was covalently immobilized onto titanium coatings to improve their biological properties. Diffuse reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were employed to characterize the HyA-modified titanium coating. HyA-modified titanium coatings possess better cell-material interaction, and human mesenchymal stem cells present good adhesive morphologies on the surface of TC-AAH. The results of subsequent cellular evaluation showed that the immobilization of HyA on titanium coatings could improve hMSC attachment, proliferation, and differentiation. In vivo evaluation of implants in rabbit femur condyle defect model showed improvements of early osseointegration and bone-to-implant contact of TC-AAH. In conclusion, immobilization of HyA could improve biological properties of titanium coatings.
authors.affiliation: a Shanghai Key Laboratory of Orthopedic Implant , Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine , Shanghai 200011 , China.
authors.forename: Haiyong
authors.initials: H
authors.lastname: Ao
authors.forename: Youtao
authors.initials: Y
authors.lastname: Xie
authors.forename: An
authors.initials: A
authors.lastname: Qin
authors.forename: Heng
authors.initials: H
authors.lastname: Ji
authors.forename: Shengbing
authors.initials: S
authors.lastname: Yang
authors.forename: Liping
authors.initials: L
authors.lastname: Huang
authors.forename: Xuebin
authors.initials: X
authors.lastname: Zheng
authors.forename: Tingting
authors.initials: T
authors.lastname: Tang
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005269:Femur / Q000187:drug effects / Q000503:physiopathology; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D018613:Microscopy, Confocal; D016348:Osseointegration / Q000187:drug effects; D056951:Photoelectron Spectroscopy; D016062:Porosity; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D006820:Hyaluronic Acid; D014025:Titanium
keywords: biological properties; covalent immobilization; hyaluronic acid; osteointegration; titanium coating