title: Methuselah-like genes affect development, stress resistance, lifespan and reproduction in Tribolium castaneum.
abstract: Methuselah (Mth) is associated with lifespan, stress resistance and reproduction in Drosophila melanogaster, but Mth is not present in nondrosophiline insects. A number of methuselah-likes (mthls) have been identified in nondrosophiline insects, but it is unknown whether the functions of mth are shared by mthls or are divergent from them. Five mthls have been identified in Tribolium castaneum. Although they have different developmental expression patterns, they all enhance resistance to starvation. Only mthl1 and mthl2 enhance resistance to high temperature, whereas mthl4 and mthl5 negatively regulate oxidative stress in T. castaneum. Unlike in the fly with mth mutation, knockdown of mthls, except mthl3, shortens the lifespan of T. castaneum. Moreover, mthl1 and mthl2 are critical for Tribolium development. mthl1 plays important roles in larval and pupal development and adult eclosion, while mthl2 is required for eclosion. Moreover, mthl1 and mthl2 silencing reduces the fertility of T. castaneum, and mthl1 and mthl4 are also essential for embryo development. In conclusion, mthls have a significant effect on insect development, lifespan, stress resistance and reproduction. These results provide experimental evidence for functional divergence among mthls/mth and clues for the signal transduction of Mthls.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China.
authors.forename: Chengjun
authors.initials: C
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Yun
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Sang
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.forename: Xingxing
authors.initials: X
authors.lastname: Hu
authors.forename: Bin
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008136:Longevity; D060888:Real-Time Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D012098:Reproduction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological; D014231:Tribolium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
chemical_list: D019476:Insect Proteins; D043562:Receptors, G-Protein-Coupled
keywords: Mthl; Tribolium castaneum; lifespan; reproduction; stress resistance