title: Dietary effect of Sargassum wightii fucoidan to enhance growth, prophenoloxidase gene expression of Penaeus monodon and immune resistance to Vibrio parahaemolyticus.
abstract: The polysaccharide fucoidan from brown seaweed Sargassum wightii was extracted and it was incorporated with pellet diets at three concentrations (0.1, 0.2 & 0.3%). The fucoidan incorporated diets were fed to shrimp Penaeus monodon for 60 days and the growth performance was assessed. The weight gain and SGR of control group was 6.83 g and 9.72%, respectively, but the weight gain and SGR of various concentrations (0.1-0.3%) of fucoidan incorporated diets fed groups of shrimp was increased from 7.30 to 8.20 g and 9.83 to 10.03%, respectively. After 60 days of feeding experiment, the relative quantification of prophenoloxidase gene of experimental groups over control group was analysed by RT-PCR and it was ranged between 2.13 and 7.95 fold increase within 33.52-34.61 threshold cycles, respectively at 0.1-0.3% concentrations of fucoidan. After 60 days of feeding experiment, the P. monodon were challenged with shrimp pathogen Vibrio parahaemolyticus and the mortality percentage was recorded daily up to 21 days. The reduction in mortality percentage of experimental groups over control group was recorded from 44.56 to 72.79%, respectively in 0.1-0.3% of fucoidan incorporated diets fed groups. During challenge experiment, all the immunological parameters such as THC, prophenoloxidase activity, respiratory burst activity, superoxide dismutase activity, phagocytic activity, bactericidal activity and bacterial clearance ability of experimental groups were significantly (P < 0.05) increased than control group. The V. parahaemolyticus load was enumerated from the infected shrimp at every 10 days intervals during challenge experiment. In control group, the Vibrio load was increased in hepatopancreas and muscle tissues from 10th to 21st days of challenge test. But in the experimental groups, the Vibrio load in both the tissues decreased positively from 10th to 21st days of challenge duration. It is concluded that the S. wightii fucoidan had enhanced the innate immunity and increased resistance to V. parahaemolyticus infection in P. monodon.
authors.affiliation: Marine Biotechnology Division, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam 629 502, Kanyakumari District, Tamilnadu, India.
authors.forename: Madasamy
authors.initials: M
authors.lastname: Sivagnanavelmurugan
authors.affiliation: Marine Biotechnology Division, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam 629 502, Kanyakumari District, Tamilnadu, India.
authors.forename: Bergmans Jude
authors.initials: BJ
authors.lastname: Thaddaeus
authors.affiliation: Marine Biotechnology Division, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam 629 502, Kanyakumari District, Tamilnadu, India.
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Palavesam
authors.affiliation: Marine Biotechnology Division, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam 629 502, Kanyakumari District, Tamilnadu, India. Electronic address: gimmas@gmail.com.
authors.forename: Grasian
authors.initials: G
authors.lastname: Immanuel
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight; D004156:Catechol Oxidase / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004792:Enzyme Precursors / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D033561:Penaeidae / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000276:immunology / Q000382:microbiology*; D011134:Polysaccharides / Q000032:analysis / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044647:Sargassum / Q000737:chemistry; D014736:Vibrio parahaemolyticus / Q000276:immunology*
chemical_list: D017931:DNA Primers; D004792:Enzyme Precursors; D011134:Polysaccharides; C007789:fucoidan; C038451:pro-phenoloxidase; D004156:Catechol Oxidase
keywords: Fucoidan; Penaeus monodon; Prophenoloxidase gene; Sargassum wightii; Vibrio parahaemolyticus