title: The mechanism of coupling between electron transfer and proton translocation in respiratory complex I.
abstract: NADH-ubiquinone oxidoreductase (complex I) is the first and largest enzyme in the respiratory chain of mitochondria and many bacteria. It couples the transfer of two electrons between NADH and ubiquinone to the translocation of four protons across the membrane. Complex I is an L-shaped assembly formed by the hydrophilic (peripheral) arm, containing all the redox centres performing electron transfer and the membrane arm, containing proton-translocating machinery. Mitochondrial complex I consists of 44 subunits of about 1 MDa in total, whilst the prokaryotic enzyme is simpler and generally consists of 14 conserved "core" subunits. Recently we have determined the first atomic structure of the entire complex I, using the enzyme from Thermus thermophilus (536 kDa, 16 subunits, 9 Fe-S clusters, 64 TM helices). Structure suggests a unique coupling mechanism, with redox energy of electron transfer driving proton translocation via long-range (up to ~200 Å) conformational changes. It resembles a steam engine, with coupling elements (akin to coupling rods) linking parts of this molecular machine.
authors.affiliation: Medical Research Council Mitochondrial Biology Unit, Hills road, Cambridge, CB2 0XY, United Kingdom, sazanov@mrc-mbu.cam.ac.uk.
authors.forename: Leonid A
authors.initials: LA
authors.lastname: Sazanov
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D004579:Electron Transport / Q000502:physiology; D042967:Electron Transport Complex I / Q000378:metabolism*; D006801:Humans; D008928:Mitochondria / Q000201:enzymology; D024101:Mitochondrial Proteins / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D018892:Proton-Motive Force / Q000502:physiology*; D016963:Thermus thermophilus / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D024101:Mitochondrial Proteins; D009243:NAD; D042967:Electron Transport Complex I