title: The effects of isoflavones on androgens and glucocorticoids during puberty on male Wistar rats.
abstract: Isoflavones are the most common phytoestrogens found in human diets. However, it is still not clear whether isoflavones have effects on the reproductive and the endocrine systems under normal dietary intake and overdose. The aim of this study was to determine how the most important isoflavones, genistein and daidzein, affect androgen and glucorticoid levels on male prepuberal rats. A hundred and seventy-five 30-day-old male Wistar rats were dosed orally by stomach tube every day for 35 days, with saline solution, low and high doses of genistein, daidzein and a mixture of both. Serum samples were analysed by an enzyme immunoassay for hormone determinations. In control group, there was a peak of testosterone (T) and dihydrotestosterone levels associated to the onset of puberty, at the third week. However, in low-dose groups, the same peak was found at the fourth week (p < 0.05), indicating a delay in the onset of puberty in these groups. Moreover, high doses groups serum androgen levels were significantly lower (p < 0.05) than the control group from the first week until fifth week. This fact was supported by a epididymal histological analysis that indicate in low doses there were several content of spermatozoa at fourth week and in high doses there were few content of spermatozoa. Besides, corticosterone levels followed the same pattern of androgens in all groups. We can conclude that oral administration of isoflavones in male rats decreased the secretion of androgens and glucocorticoids causing a delay in the onset of puberty and may cause physiological and developmental problems.
authors.affiliation: Dpto Fisiologia Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Caceres
authors.affiliation: Dpto Fisiologia Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Silvan
authors.affiliation: Dpto Fisiologia Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Martinez-Fernandez
authors.affiliation: Dpto Fisiologia Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Illera
authors.affiliation: Dpto Fisiologia Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Millan
authors.affiliation: Dpto Fisiologia Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Monsalve
authors.affiliation: Dpto Medicina y Cirugia Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Peña
authors.affiliation: Dpto Fisiologia Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Illera
mesh_terms: D000728:Androgens / Q000097:blood*; D000818:Animals; D003345:Corticosterone / Q000097:blood; D013196:Dihydrotestosterone / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004822:Epididymis / Q000166:cytology; D019833:Genistein / Q000008:administration & dosage; D005938:Glucocorticoids / Q000097:blood*; D007529:Isoflavones / Q000008:administration & dosage* / Q000009:adverse effects; D008297:Male; D048789:Phytoestrogens / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D012741:Sexual Maturation / Q000187:drug effects* / Q000502:physiology; D013076:Sperm Count; D013739:Testosterone / Q000097:blood
chemical_list: D000728:Androgens; D005938:Glucocorticoids; D007529:Isoflavones; D048789:Phytoestrogens; D013196:Dihydrotestosterone; D013739:Testosterone; C004742:daidzein; D019833:Genistein; D003345:Corticosterone