title: Toxicity of abiotic stressors to Fusarium species: differences in hydrogen peroxide and fungicide tolerance.
abstract: Stress sensitivity of three related phytopathogenic Fusarium species (Fusarium graminearum, Fusarium oxysporum and Fusarium verticillioides) to different oxidative, osmotic, cell wall, membrane, fungicide stressors and an antifungal protein (PAF) were studied in vitro. The most prominent and significant differences were found in oxidative stress tolerance: all the three F. graminearum strains showed much higher sensitivity to hydrogen peroxide and, to a lesser extent, to menadione than the other two species. High sensitivity of F. verticillioides strains was also detectable to an azole drug, Ketoconazole. Surprisingly, no or limited differences were observed in response to other oxidative, osmotic and cell wall stressors. These results indicate that fungal oxidative stress response and especially the response to hydrogen peroxide (this compound is involved in a wide range of plant-fungus interactions) might be modified on niche-specific manner in these phylogenetically related Fusarium species depending on their pathogenic strategy. Supporting the increased hydrogen peroxide sensitivity of F. graminearum, genome-wide analysis of stress signal transduction pathways revealed the absence one CatC-type catalase gene in F. graminearum in comparison to the other two species.
authors.affiliation: Hungarian Academy of Sciences Plant Protection Institute, Centre for Agricultural Research H-1525 Budapest P.O. Box 102 Hungary.
authors.forename: Emese D
authors.initials: ED
authors.lastname: Nagygyörgy
authors.affiliation: University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Sciences H-4032 Debrecen Egyetem tér 1 Hungary.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kovács
authors.affiliation: University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Sciences H-4032 Debrecen Egyetem tér 1 Hungary.
authors.forename: Eva
authors.initials: E
authors.lastname: Leiter
authors.affiliation: University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Sciences H-4032 Debrecen Egyetem tér 1 Hungary.
authors.forename: Márton
authors.initials: M
authors.lastname: Miskei
authors.affiliation: University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Sciences H-4032 Debrecen Egyetem tér 1 Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
authors.affiliation: Szent István University Mycology Group of the Hungarian Academy of Sciences, Institute of Plant Protection H-2103 Gödöllő Páter K. u. 1 Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Hornok
authors.affiliation: Hungarian Academy of Sciences Plant Protection Institute, Centre for Agricultural Research H-1525 Budapest P.O. Box 102 Hungary.
authors.forename: Attila L
authors.initials: AL
authors.lastname: Adám
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002374:Catalase / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D025141:Drug Resistance, Fungal; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D005670:Fusarium / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D017353:Gene Deletion; D016681:Genome, Fungal; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007654:Ketoconazole / Q000494:pharmacology; D018384:Oxidative Stress; D010802:Phylogeny; D013045:Species Specificity; D024483:Vitamin K 3 / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; C097609:antifungal protein, Aspergillus; D024483:Vitamin K 3; D006861:Hydrogen Peroxide; D002374:Catalase; D007654:Ketoconazole
keywords: Fusarium; Ketoconazole; abiotic stress tolerance; catalase; hydrogen peroxide; niche-specific adaptation; oxidative stress