title: RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit.
abstract: The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring the three different RNAi pOpOff2 constructs bearing the 1-aminocyclopropane-1-carboxylic acid oxidase gene. A total of 176 putative transformed lines were produced from 15,000 calli transformed, selected, then regenerated on medium supplemented with kanamycin. Integration and expression of the targeted gene in putatively transformed lines were verified by PCR and real-time RT-PCR. Confined field evaluation of a total of 31 putative transgenic lines planted showed a knockdown expression of the targeted ACO1 and ACO2 genes in 13 lines, which required more than 8 days to achieve the full yellow colour (Index 6). Fruits harvested from lines pRNAiACO2 L2-9 and pRNAiACO1 L2 exhibited about 20 and 14 days extended post-harvest shelf life to reach Index 6, respectively. The total soluble solids contents of the fruits ranged from 11 to 14° Brix, a range similar to fruits from non-transformed, wild type seed-derived plants.
authors.affiliation: Malaysian Agricultural Research and Development Institute (MARDI), P.O. Box 12301, Kuala Lumpur 50774, Malaysia. lynn@mardi.gov.my.
authors.forename: Rogayah
authors.initials: R
authors.lastname: Sekeli
authors.affiliation: Department of Microbiology, Faculty of Biotechnology & Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor Darul Ehsan, Malaysia. janna@upm.edu.my.
authors.forename: Janna Ong
authors.initials: JO
authors.lastname: Abdullah
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology & Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor Darul Ehsan, Malaysia. parameswari@upm.edu.my.
authors.forename: Parameswari
authors.initials: P
authors.lastname: Namasivayam
authors.affiliation: Malaysian Agricultural Research and Development Institute (MARDI), P.O. Box 12301, Kuala Lumpur 50774, Malaysia. pauziah@mardi.gov.my.
authors.forename: Pauziah
authors.initials: P
authors.lastname: Muda
authors.affiliation: Malaysian Agricultural Research and Development Institute (MARDI), P.O. Box 12301, Kuala Lumpur 50774, Malaysia. uab@mardi.gov.my.
authors.forename: Umi Kalsom
authors.initials: UK
authors.lastname: Abu Bakar
authors.affiliation: Malaysian Agricultural Research and Development Institute (MARDI), P.O. Box 12301, Kuala Lumpur 50774, Malaysia. cywee@mardi.gov.my.
authors.forename: Wee Chien
authors.initials: WC
authors.lastname: Yeong
authors.affiliation: Ministry of Science, Technology and Innovation (MOSTI), Putrajaya 62574, Federal Territory, Malaysia. vilasini@mosti.gov.my.
authors.forename: Vilasini
authors.initials: V
authors.lastname: Pillai
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D029441:Carica / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D005638:Fruit / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D034622:RNA Interference / Q000502:physiology
chemical_list: D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase