title: miR-375 inhibits the proliferation of gastric cancer cells by repressing ERBB2 expression.
abstract: MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate the expression of targeted genes in a post-transcriptional manner. Increasing evidence indicates that miRNAs play important roles in cancer pathogenesis, including apoptosis, proliferation and differentiation, as oncogenes or tumor suppressors. Previously, miR-375 was shown to be involved in human gastric cancer, however, the mechanism remains poorly understood. In the present study, miR-375 was shown to be downregulated in gastric cancer tissues, particularly human epidermal growth factor receptor 2 (ERBB2)-positive gastric cancer tissues. Identified by dual luciferase assays and western blot analysis, ERBB2 was demonstrated to be a target gene of miR-375. In addition, miR-375 overexpression suppressed the proliferation of human gastric cancer cellsin vitroand the suppression effect was restored by ERBB2 overexpression. Thus, the results of the present study indicate that miR-375 is associated with human gastric carcinogenesis by targeting ERBB2. Therefore, miR-375 may be used as a potential clinical classification marker and therapeutic target for human gastric cancer.
authors.affiliation: Department of General Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201112, P.R. China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Shen
authors.affiliation: Department of General Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201112, P.R. China.
authors.forename: Zi-Zhen
authors.initials: ZZ
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201112, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201112, P.R. China.
authors.forename: En-Hao
authors.initials: EH
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201112, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
keywords: gastric cancer; human epidermal growth factor receptor 2; microRNA; proliferation