title: Potential of essential oils for protection of grains contaminated by aflatoxin produced by Aspergillus flavus.
abstract: Aflatoxin B1 (AFB1) is a highly toxic and carcinogenic metabolite produced by Aspergillus species on food and agricultural commodities. Inhibitory effects of essential oils of Ageratum conyzoides (mentrasto) and Origanum vulgare (oregano) on the mycelial growth and aflatoxin B1 production by Aspergillus flavus have been studied previously in culture medium. The aim of this study was to evaluate aflatoxin B1 production by Aspergillus flavus in real food systems (corn and soybean) treated with Ageratum conyzoides (mentrasto) and Origanum vulgare (oregano) essential oils. Samples with 60 g of the grains were treated with different volumes of essential oils, 200, 100, 50, and 10 μL for oregano and 50, 30, 15, and 10 μL for mentrasto. Fungal growth was evaluated by disk diffusion method. Aflatoxin B1 production was evaluated inoculating suspensions of A. flavus containing 1.3 × 10(5) spores/mL in 60 g of grains (corn and soybeans) after adjusting the water activity at 0.94. Aflatoxin was quantified by photodensitometry. Fungal growth and aflatoxin production were inhibited by essential oils, but the mentrasto oil was more effective in soybeans than that of oregano. On the other hand, in corn samples, the oregano essential oil was more effective than that of mentrasto. Chemical compositions of the essential oils were also investigated. The GC/MS oils analysis showed that the main component of mentrasto essential oil is precocene I and of the main component of oregano essential oil is 4-terpineol. The results indicate that both essential oils can become an alternative for the control of aflatoxins in corn and soybeans.
authors.affiliation: Centro de Pesquisa e Desenvolvimento em Sanidade Animal, Instituto Biológico São Paulo, Brazil.
authors.forename: Renata H
authors.initials: RH
authors.lastname: Esper
authors.affiliation: Centro de Pesquisa e Desenvolvimento em Sanidade Animal, Instituto Biológico São Paulo, Brazil.
authors.forename: Edlayne
authors.initials: E
authors.lastname: Gonçalez
authors.affiliation: Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais, Instituto Agronômico Campinas, Brazil.
authors.forename: Marcia O M
authors.initials: MO
authors.lastname: Marques
authors.affiliation: Departamento de Ciências Exatas e Tecnológicas, Universidade Estadual de Santa Cruz Ilhéus, Brazil.
authors.forename: Roberto C
authors.initials: RC
authors.lastname: Felicio
authors.affiliation: Centro de Pesquisa e Desenvolvimento em Sanidade Animal, Instituto Biológico São Paulo, Brazil.
authors.forename: Joana D
authors.initials: JD
authors.lastname: Felicio
keywords: Ageratum conyzoides; Origanum vulgare; corn; essential oil; mycotoxins; soybeans