title: Radiochemical pharmacokinetic profile of P10 peptide with antifungal properties.
abstract: Paracoccidioidomycosis (PCM) is a chronic granulomatous disease that is caused by the thermally dimorphic fungus Paracoccidioides brasiliensis. It is endemic in some countries of Latin America and can cause a high-burden fungal infection with significant morbidity and mortality. The peptide P10, which demonstrates immune protection against experimental PCM, was radiolabeled with a radioisotope and evaluated in vivo. The radiolabeling was conducted to trace the pharmacokinetics of the molecule in principal organs and tissues. This was achieved with high radiochemical purity. Biodistribution and scintigraphic imaging showed fast blood clearance that was mainly renal; however, hepatobiliar excretion was also, with marked uptake in cervical lymph nodes. This profile may be useful for the development of a prophylactic drug or vaccine for patients exposed to PCM.
authors.affiliation: Radiopharmacy, Institute of Energy and Nuclear Research, Sao Paulo faintuch@ipen.br blfaintuch@hotmail.com.
authors.forename: Bluma L
authors.initials: BL
authors.lastname: Faintuch
authors.affiliation: Radiopharmacy, Institute of Energy and Nuclear Research, Sao Paulo.
authors.forename: Erica A
authors.initials: EA
authors.lastname: Oliveira
authors.affiliation: Institute of Biomedical Sciences, Department of Microbiology, University of São Paulo.
authors.forename: Julian E
authors.initials: JE
authors.lastname: Munõz
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo.
authors.forename: Luiz R
authors.initials: LR
authors.lastname: Travassos
authors.affiliation: Institute of Biomedical Sciences, Department of Microbiology, University of São Paulo Laboratory of Medical Mycology, Instituto de Medicina Tropical Sao Paulo, IMTSP Laboratorio de Investigacao Medica 53/LIM 53, University of São Paulo, Brazil.
authors.forename: Carlos P
authors.initials: CP
authors.lastname: Taborda
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000493:pharmacokinetics*; D002614:Chelating Agents / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010228:Paracoccidioides / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000382:microbiology* / Q000517:prevention & control; D010455:Peptides / Q000493:pharmacokinetics*
chemical_list: D000935:Antifungal Agents; D002614:Chelating Agents; D010455:Peptides
keywords: P. brasiliensis; P10 peptide; biodistribution evaluation; radiolabeling