title: Consecutive Intake of Fermented Milk Containing Bifidobacterium breve Strain Yakult and Galacto-oligosaccharides Benefits Skin Condition in Healthy Adult Women.
abstract: A double-blind, placebo-controlled, randomized trial was conducted to investigate the beneficial effect of probiotic and prebiotic fermented milk on the skin of healthy adult women. Forty healthy Japanese adult female volunteers with healthy skin randomly received either a bottle of probiotic and prebiotic fermented milk containing Bifidobacterium breve strain Yakult and galacto-oligosaccharides (GOS) (active group) or a non-fermented placebo milk containing neither probiotics nor GOS (placebo group) daily for 4 weeks. Before and after intake, hydration levels and cathepsin L-like activity in the stratum corneum and phenol levels in the serum and urine were determined. After intake, the hydration level of the stratum corneum decreased significantly in only the placebo group and was significantly lower than in the active group (p=0.031). Cathepsin L-like activity, an indicator of keratinocyte differentiation, was significantly increased in the active group (p=0.027). Serum and urine phenol levels decreased significantly in the active group (p=0.014, p=0.002, respectively), and serum phenol levels were significantly lower in the active group compared with the placebo group (p=0.006). The consecutive intake of probiotic and prebiotic fermented milk can benefit skin condition without dryness and decrease the levels of phenol production by gut bacteria in healthy adult women.
authors.affiliation: Yakult Central Institute for Microbiological Research, Kunitachi 186-8650, Japan.
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Kano
authors.affiliation: Yakult Central Institute for Microbiological Research, Kunitachi 186-8650, Japan.
authors.forename: Norie
authors.initials: N
authors.lastname: Masuoka
authors.affiliation: Yakult Central Institute for Microbiological Research, Kunitachi 186-8650, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kaga
authors.affiliation: Yakult Central Institute for Microbiological Research, Kunitachi 186-8650, Japan.
authors.forename: Saho
authors.initials: S
authors.lastname: Sugimoto
authors.affiliation: Yakult Central Institute for Microbiological Research, Kunitachi 186-8650, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Iizuka
authors.affiliation: Yakult Central Institute for Microbiological Research, Kunitachi 186-8650, Japan.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Manabe
authors.affiliation: Yakult Central Institute for Microbiological Research, Kunitachi 186-8650, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Sone
authors.affiliation: Faculty of Research and Development, Yakult Honsha, Shimbashi, Tokyo 105-8660, Japan.
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Oeda
authors.affiliation: Faculty of Research and Development, Yakult Honsha, Shimbashi, Tokyo 105-8660, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Nonaka
authors.affiliation: Yakult Central Institute for Microbiological Research, Kunitachi 186-8650, Japan.
authors.forename: Kouji
authors.initials: K
authors.lastname: Miyazaki
authors.affiliation: Yakult Central Institute for Microbiological Research, Kunitachi 186-8650, Japan.
authors.forename: Fumiyasu
authors.initials: F
authors.lastname: Ishikawa
keywords: Bifidobacterium; galacto oligosaccharides; phenol; prebiotics; probiotics; skin condition; skin hydration