title: Transcriptomic effects of estradiol treatment on cultured human uterine smooth muscle cells.
abstract: Contractility of the myometrial smooth muscle cells during the estrous cycle and pregnancy is modulated by estrogen but the temporal expression of estrogen (relative to progesterone) and the type of contraction involved are distinctly different in pregnancy and estrous. This in vitro cell culture study investigated the global gene expression profile of human uterine smooth muscle cells (hUtSMCs) following 17β-estradiol (E2) treatment. In response to E2 treatment 540 genes, many of which have not been previously described as estrogen responsive, were identified as significantly differentially expressed. These genes are involved in biological processes that include muscle contraction, cell migration and adhesion, apoptosis and phosphorylation. Evidence from this study suggests that 17β-estradiol may have effects that are contrary to an overall contraction phenotype. The hUtSMC in vitro culture system is a useful model to investigate steroid effects on smooth muscle cells and may provide additional clues as to how smooth muscle cells behave in vivo.
authors.affiliation: National Centre for Biomedical Engineering Science, NUI Galway, Galway, Ireland.
authors.forename: Sreenath
authors.initials: S
authors.lastname: Chandran
authors.affiliation: National Centre for Biomedical Engineering Science, NUI Galway, Galway, Ireland.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Cairns
authors.affiliation: National Centre for Biomedical Engineering Science, NUI Galway, Galway, Ireland.
authors.forename: Margaret
authors.initials: M
authors.lastname: O'Brien
authors.affiliation: National Centre for Biomedical Engineering Science, NUI Galway, Galway, Ireland. Electronic address: terry.smith@nuigalway.ie.
authors.forename: Terry J
authors.initials: TJ
authors.lastname: Smith
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D030762:Estrous Cycle; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D046228:Microarray Analysis; D009119:Muscle Contraction / Q000187:drug effects; D009215:Myometrium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger; D004958:Estradiol; D051997:ADP-ribosyl Cyclase 1
keywords: 17β-Estradiol; Estrous; Global gene expression; Myometrium; Pregnancy; Smooth muscle contraction