title: Aspartame induces angiogenesis in vitro and in vivo models.
abstract: Angiogenesis is the process of generating new blood vessels from preexisting vessels and is considered essential in many pathological conditions. The purpose of the present study is to evaluate the effect of aspartame on angiogenesis in vivo chick chorioallantoic membrane (CAM) and wound-healing models as well as in vitro 2,3-bis-2H-tetrazolium-5-carboxanilide (XTT) and tube formation assays. In CAM assay, aspartame increased angiogenesis in a concentration-dependent manner. Compared with the control group, aspartame has significantly increased vessel proliferation (p < 0.001). In addition, in vivo rat model of skin wound-healing study showed that aspartame group had better healing than control group, and this was statistically significant at p < 0.05. There was a slight proliferative effect of aspartame on human umbilical vein endothelial cells on XTT assay in vitro, but it was not statistically significant; and there was no antiangiogenic effect of aspartame on tube formation assay in vitro. These results provide evidence that aspartame induces angiogenesis in vitro and in vivo; so regular use may have undesirable effect on susceptible cases.
authors.affiliation: Department of Biochemistry, Gülhane Military Medical Academy, Ankara, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Yesildal
authors.affiliation: Department of Biochemistry, Gülhane Military Medical Academy, Ankara, Turkey.
authors.forename: F N
authors.initials: FN
authors.lastname: Aydin
authors.affiliation: Department of Pathology, Gülhane Military Medical Academy, Ankara, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Deveci
authors.affiliation: School of Medicine, Gülhane Military Medical Academy, Ankara, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Tekin
authors.affiliation: Department of Biochemistry, Gülhane Military Medical Academy, Ankara, Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Aydin
authors.affiliation: National Nanotechnology Research Center, Bilkent University, Ankara, Turkey.
authors.forename: R
authors.initials: R
authors.lastname: Mammadov
authors.affiliation: School of Medicine, Gülhane Military Medical Academy, Ankara, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Fermanli
authors.affiliation: Department of Haematology, Gülhane Military Medical Academy, Ankara, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Avcu
authors.affiliation: Department of Public Health and Biostatistics, Gülhane Military Medical Academy, Ankara, Turkey.
authors.forename: C H
authors.initials: CH
authors.lastname: Acikel
authors.affiliation: Department of Biochemistry, Gülhane Military Medical Academy, Ankara, Turkey chem352000@yahoo.com.
authors.forename: T
authors.initials: T
authors.lastname: Ozgurtas
mesh_terms: D000818:Animals; D001218:Aspartame / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000502:physiology; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D017207:Rats, Sprague-Dawley; D013549:Sweetening Agents / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D013549:Sweetening Agents; D001218:Aspartame
keywords: Aspartame; CAM assay; angiogenesis; skin wound healing; tube formation