title: Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.
abstract: Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and 1% (v/v) methanol. The Discovery HS F5 column with a pentafluorophenyl (PFP) stationary phase gave sharp peaks and full separation of the arsenic species in 5min, and other PFP columns showed lower performance. This separation method was applied to arsenic species analysis in rice. The extraction of arsenic from rice samples was performed using 0.15M nitric acid. The methodology was validated by use of certified reference materials, NMIJ CRM 7503-a and NIST SRM 1568a, and extremely low arsenic rice samples as blank samples.
authors.affiliation: National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Ibaraki, Japan. Electronic address: kojibaba@affrc.go.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Baba
authors.affiliation: National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Ibaraki, Japan.
authors.forename: Tomohito
authors.initials: T
authors.lastname: Arao
authors.affiliation: National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Ibaraki, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamaguchi
authors.affiliation: National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Ibaraki, Japan.
authors.forename: Eiki
authors.initials: E
authors.lastname: Watanabe
authors.affiliation: National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Ibaraki, Japan.
authors.forename: Heesoo
authors.initials: H
authors.lastname: Eun
authors.affiliation: National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Ibaraki, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Ishizaka
mesh_terms: D001151:Arsenic / Q000737:chemistry / Q000302:isolation & purification*; D001152:Arsenicals / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D005466:Fluorocarbons / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D012275:Oryza / Q000737:chemistry*
chemical_list: D001152:Arsenicals; D005466:Fluorocarbons; C033093:perfluoropropionic acid; D001151:Arsenic
keywords: Arsenic speciation; HPLC–ICPMS; Pentafluorophenyl column; Rice; Volatile mobile phase