title: Targeted purification of SnAvi-tagged proteins.
abstract: Tandem affinity purification (TAP) is a powerful technique to identify protein complex members. The modular composition of TAP-tags allows two sequential protein enrichment steps and thereby drastically reduces the amount of contaminants. Here, we describe the application of the SnAvi-tag-a TAP-tag useful in different expression systems. Due to its modular composition, this tag is multifunctional and facilitates among others the in vivo visualization of tagged proteins and their cell type specific activation.
authors.affiliation: Institute for Biology 3, University of Freiburg, Schänzlestr. 4, 79104, Freiburg, Germany, ursula_schaeffer@hotmail.com.
authors.forename: Ursula
authors.initials: U
authors.lastname: Schäffer
authors.forename: Ralf
authors.initials: R
authors.lastname: Baumeister
authors.forename: Ekkehard
authors.initials: E
authors.lastname: Schulze
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D000939:Epitopes / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D009928:Organ Specificity; D060066:Protein Interaction Maps; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D053719:Tandem Mass Spectrometry
chemical_list: D000939:Epitopes; D011994:Recombinant Proteins