title: Resins-based denture soft lining materials modified by chlorhexidine salt incorporation: an in vitro analysis of antifungal activity, drug release and hardness.
abstract: OBJECTIVE
  To evaluate the in vitro growth inhibition of Candida albicans, the rate of chlorhexidine release and shore A hardness from resins-based denture soft lining materials modified by chlorhexidine diacetate (CDA) or chlorhexidine hydrochloride (CHC) incorporation.
  
  
  METHODS
  Resin discs were prepared from soft denture liners based on poly (methyl methacrylate) (PMMA) or poly (ethyl methacrylate) (PEMA) containing 0.5, 1.0 and 2.0 wt.% of CDA or CHC. For antifungal activity resin discs were placed on agar plates inoculated with C. albicans, after 48 h at 37°C the diameters of inhibition zones were measured. For the chlorhexidine release, discs were immersed into distilled water at 37°C, and spectral measurements were made after 48 h. Shore A hardness was evaluated at the baseline, 2 and 7 days, using 6mm thick rectangular specimens also immersed into distilled water at 37°C. Data were statistically processed by SigmaStat software using ANOVA and all pairwise multiple comparison procedures was done using the Holm-Sidak method, with α=0.05 (p<0.001).
  
  
  RESULTS
  CDA added to PMMA soft liner and PEMA soft liner had a dose-related inhibitory effect on C. albicans and on chlorhexidine release rate (p<0.001). The PMMA and PEMA hardness increased statistically by time but not for the different CDA concentrations. CHC had no inhibitory effect on C. albicans.
  
  
  CONCLUSIONS
  Chlorhexidine diacetate released from resins-based soft lining materials can be convenient to reduce the biofilm development on the material surface and treat denture stomatitis, without depending on patient compliance.
authors.affiliation: Department of Prosthodontics, Dental School, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. Electronic address: martinnabertolini@gmail.com.
authors.forename: Martinna M
authors.initials: MM
authors.lastname: Bertolini
authors.affiliation: Department of Clinics, Pediatric Dentistry, School of Dentistry, Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil.
authors.forename: Maristela B
authors.initials: MB
authors.lastname: Portela
authors.affiliation: Institute of Microbiology Professor Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: José Alexandre R
authors.initials: JA
authors.lastname: Curvelo
authors.affiliation: Institute of Microbiology Professor Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Rosangela M A
authors.initials: RM
authors.lastname: Soares
authors.affiliation: Department of Prosthodontics, Dental School, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Eduardo J V
authors.initials: EJ
authors.lastname: Lourenço
authors.affiliation: Department of Prosthodontics, Dental School, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Telles
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology; D002710:Chlorhexidine / Q000737:chemistry* / Q000494:pharmacology; D003822:Denture Liners; D065546:Drug Liberation; D006245:Hardness Tests; D066298:In Vitro Techniques; D008768:Methylmethacrylates; D019904:Polymethyl Methacrylate
chemical_list: D000935:Antifungal Agents; D008768:Methylmethacrylates; C009931:poly(ethylmethacrylate); D019904:Polymethyl Methacrylate; D002710:Chlorhexidine
keywords: Antifungal activity; Candida albicans; Chlorhexidine; Denture soft lining materials; Drug release; Hardness