title: Evidence for lysine acetylation in the coat protein of a polerovirus.
abstract: Virions of the RPV strain of Cereal yellow dwarf virus-RPV were purified from infected oat tissue and analysed by MS. Two conserved residues, K147 and K181, in the virus coat protein, were confidently identified to contain epsilon-N-acetyl groups. While no functional data are available for K147, K181 lies within an interfacial region critical for virion assembly and stability. The signature immonium ion at m/z 126.0919 demonstrated the presence of N-acetyllysine, and the sequence fragment ions enabled an unambiguous assignment of the epsilon-N-acetyl modification on K181. We hypothesize that selection favours acetylation of K181 in a fraction of coat protein monomers to stabilize the capsid by promoting intermonomer salt bridge formation.
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA.|Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.|USDA-Agricultural Research Service, Ithaca, NY 14853, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Cilia
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, WA 98109, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Johnson
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Sweeney
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA.|USDA-Agricultural Research Service, Ithaca, NY 14853, USA.
authors.forename: Stacy L
authors.initials: SL
authors.lastname: DeBlasio
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, WA 98109, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Bruce
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, WA 98109, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: MacCoss
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.|USDA-Agricultural Research Service, Ithaca, NY 14853, USA.
authors.forename: Stewart M
authors.initials: SM
authors.lastname: Gray
mesh_terms: D000107:Acetylation; D036022:Capsid Proteins / Q000378:metabolism*; D052679:Luteoviridae / Q000737:chemistry / Q000502:physiology*; D008239:Lysine / Q000378:metabolism*; D013058:Mass Spectrometry; D011499:Protein Processing, Post-Translational
chemical_list: D036022:Capsid Proteins; D008239:Lysine