title: Trehalose enhancing microbial electrolysis cell for hydrogen generation in low temperature (0 °C).
abstract: This work explored the feasibility of a method combining physical (sonication and base) and biological (partial fermentation) processes for sludge treatment and the effects of trehalose on the hydrogen generation of microbial electrolysis cell at 0 °C. The results demonstrated that the above pretreatment method was favorable, which promoted organics decomposing into lower molecular weight matter. The promotion of trehalose for MEC efficiency was obvious and the optimal concentration of trehalose was 50 mmol/L. With this concentration, the highest hydrogen recovery rate was 0.25 m(3)-H₂/-m(3)-reactor per day. Coulomb efficiency and energy recovery efficiency were 46.4% and 203%, respectively. Further, the consumption order of mixed substances was VFAs>proteins>carbohydrates. For microorganism community, SEM photographs illustrated that the selectivity of environmental temperature for the species of anode bacteria was strong and denaturing gradient gel electrophoresis indicated that Microbacterium and Proteobacteria were the two main species and Proteobacteria may be one of the species that produced electrons.
authors.affiliation: State Key Laboratory of Urban Water Energy and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, PR China; The Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hong Kong; Peng Wei Petrochemical Co., LTD, Longqiao Industrial Park, Fuling District, Chongqing 408121, PR China.
authors.forename: Linji
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China. Electronic address: wzliu@rcees.ac.cn.
authors.forename: Wenzong
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Urban Water Energy and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, PR China.
authors.forename: Yining
authors.initials: Y
authors.lastname: Wu
authors.affiliation: The Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hong Kong; Peng Wei Petrochemical Co., LTD, Longqiao Industrial Park, Fuling District, Chongqing 408121, PR China.
authors.forename: Poheng
authors.initials: P
authors.lastname: Lee
authors.affiliation: State Key Laboratory of Urban Water Energy and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, PR China; Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
authors.forename: Aijie
authors.initials: A
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Energy and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
mesh_terms: D001674:Bioelectric Energy Sources; D019149:Bioreactors; D004572:Electrolysis / Q000379:methods*; D005285:Fermentation; D006859:Hydrogen / Q000737:chemistry*; D020560:Proteobacteria / Q000378:metabolism; D012722:Sewage / Q000737:chemistry*; D013696:Temperature; D014199:Trehalose / Q000737:chemistry*; D018505:Waste Management / Q000379:methods
chemical_list: D012722:Sewage; D006859:Hydrogen; D014199:Trehalose
keywords: Hydrogen; Low temperature; Microbial electrolysis cell; Sludge; Trehalose