title: Negative neuronal differentiation of human adipose-derived stem cell clones.
abstract: OBJECTIVE
  Adipose mesenchymal stem cells are a heterogeneous population. Therefore, the question posed in this study is whether the heterogenic differentiation potential exhibited by the different clones toward mesodermic lineages can be extended to nonmesodermic lineages, such as the neuroectoderm.
  
  
  METHODS
  Different single cell clones of human adipose mesenchymal stem cells from the same donor were isolated. Neuronal plasticity of the clones was assessed according to the pattern DNA methylation, gene expression and intracellular calcium responses.
  
  
  RESULTS
  Under neurogenic culture conditions, clones presented variable expression of neuronal-specific genes, but still expressed osteogenic markers. No calcium response was exhibited in response to KCl incubation. The DNA methylation profile presented a very similar pattern in neuroectoderm gene promoters.
  
  
  CONCLUSIONS
  Data indicate that there are no significant differences between the undifferentiated and supposedly neuronal-differentiated mesenchymal cells.
authors.affiliation: Biochemistry & Cell Therapy Unit, Institute of Bioengineering, University Miguel Hernandez, 03202-Elche, Alicante, Spain.
authors.forename: María I
authors.initials: MI
authors.lastname: Arribas
authors.forename: Ana B
authors.initials: AB
authors.lastname: Ropero
authors.forename: Juan A
authors.initials: JA
authors.lastname: Reig
authors.forename: Mario F
authors.initials: MF
authors.lastname: Fraga
authors.forename: Agustin F
authors.initials: AF
authors.lastname: Fernandez
authors.forename: Alfredo
authors.initials: A
authors.lastname: Santana
authors.forename: Enrique
authors.initials: E
authors.lastname: Roche
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D019175:DNA Methylation / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D054258:Neural Plate / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers
keywords: adipose tissue; adult stem cells; cell therapy; neurodegenerative diseases; neuron