title: Molecular survey of Babesia gibsoni using Haemaphysalis longicornis collected from dogs and cats in Japan.
abstract: A nationwide survey of Babesia gibsoni using Haemaphysalis longicornis collected from dogs and cats in Japan was conducted using molecular methods. A total of 1,341 H. longicornis, including 305 females, 14 males, 332 nymphs and 690 larvae (153 pools) from 44 prefectures, were examined by B. gibsoni-targeted PCR. Partial sequence analysis revealed that 12 of 13 positive samples sequenced, including samples from Tottori, Hiroshima, Yamaguchi, Tokushima, Ehime and Oita prefectures (all in western Japan), were identical to B. gibsoni, and 1 sample from Kyoto Prefecture was most closely related to a Babesia species recently detected from feral raccoons in Hokkaido. H. longicornis is a candidate for transmission vector tick of the new Babesia species.
authors.affiliation: Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Iwakami
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
mesh_terms: D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification*; D001404:Babesiosis / Q000453:epidemiology / Q000469:parasitology*; D001483:Base Sequence; D002371:Cat Diseases / Q000453:epidemiology / Q000469:parasitology*; D002415:Cats; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D005260:Female; D026863:Ixodidae / Q000469:parasitology*; D007564:Japan / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D018709:Statistics, Nonparametric
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S