title: Murine spleen tissue regeneration from neonatal spleen capsule requires lymphotoxin priming of stromal cells.
abstract: Spleen is a tissue with regenerative capacity, which allows autotransplantation of human spleen fragments to counteract the effects of splenectomy. We now reveal in a murine model that transplant of neonatal spleen capsule alone leads to the regeneration of full spleen tissue. This finding indicates that graft-derived spleen stromal cells, but not lymphocytes, are essential components of tissue neogenesis, a finding verified by transplant and regeneration of Rag1KO spleen capsules. We further demonstrate that lymphotoxin and lymphoid tissue inducer cells participate in two key elements of spleen neogenesis, bulk tissue regeneration and white pulp organization, identifying a lymphotoxin-dependent pathway for neonatal spleen regeneration that contrasts with previously defined lymphotoxin-independent embryonic spleen organogenesis.
authors.affiliation: Astellas-Kyoto University (AK) Project, Graduate School of Medicine, Kyoto University, Yoshida-konoe, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Jonathan K H
authors.initials: JK
authors.lastname: Tan
authors.affiliation: Astellas-Kyoto University (AK) Project, Graduate School of Medicine, Kyoto University, Yoshida-konoe, Sakyo-ku, Kyoto 606-8501, Japan takeshi-watanabe@kitano-hp.or.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D006801:Humans; D008233:Lymphotoxin-alpha / Q000502:physiology*; D051379:Mice; D057507:Mice, 129 Strain; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D023421:Models, Animal; D012038:Regeneration / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000637:transplantation*; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology
chemical_list: D008233:Lymphotoxin-alpha