title: Preparation and sonodynamic activities of water-soluble tetra-α-(3-carboxyphenoxyl) zinc(II) phthalocyanine and its bovine serum albumin conjugate.
abstract: Sonodynamic therapy (SDT) is a new approach for cancer treatment, involving the synergistic effect of ultrasound and certain chemical compounds termed as sonosensitizers. A water-soluble phthalocyanine, namely tetra-α-(3-carboxyphenoxyl) zinc(II) phthalocyanine (ZnPcC4), has been prepared and characterized. The interactions between ZnPcC4 and bovine serum albumin (BSA) were also investigated by absorption and fluorescence spectroscopy. It was found that there were strong interactions between ZnPcC4 and BSA with a binding constant of 6.83×10(7)M(-1). A non-covalent BSA conjugate of ZnPcC4 (ZnPcC4-BSA) was prepared. Both ZnPcC4 and ZnPcC4-BSA exhibited efficient sonodynamic activities against HepG2 human hepatocarcinoma cells. Compared with ZnPcC4, conjugate ZnPcC4-BSA showed a higher sonodynamic activity with an IC50 value of 7.5μM. Upon illumination with ultrasound, ZnPcC4-BSA can induce an increase of intracellular reactive oxygen species (ROS) level, resulting in cellular apoptosis. The results suggest that the albumin conjugates of zinc(II) phthalocyanines functionalized with carboxyls can serve as promising sonosensitizers for sonodynamic therapy.
authors.affiliation: College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, China.
authors.forename: He-Nan
authors.initials: HN
authors.lastname: Xu
authors.affiliation: College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, China.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, China.
authors.forename: Bi-Yuan
authors.initials: BY
authors.lastname: Zheng
authors.affiliation: College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, China.
authors.forename: Yun-Quan
authors.initials: YQ
authors.lastname: Zheng
authors.affiliation: College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, China.
authors.forename: Mei-Rong
authors.initials: MR
authors.lastname: Ke
authors.affiliation: College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, China. Electronic address: jdhuang@fzu.edu.cn.
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Huang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002417:Cattle; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D054833:Isoindoles; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D010789:Phototherapy; D017382:Reactive Oxygen Species / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D012995:Solubility; D014464:Ultrasonic Therapy; D014867:Water / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D007211:Indoles; D054833:Isoindoles; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D014867:Water; D012710:Serum Albumin, Bovine; C013647:phthalocyanine
keywords: Phthalocyanine; Serum albumin; Sonodynamic therapy; Sonosensitizer; Ultrasound