title: Activation of innate immune system in response to lipopolysaccharide in chicken Sertoli cells.
abstract: Sertoli cells (SCs) play an important physiological role in the testis, as they support, nourish, and protect the germ cells. As protection of the developing spermatozoa is an emerging aspect of reproductive physiology, this study examined the expression pattern of innate immune-related genes, including avian β-defensins (AvBDs), Toll-like receptors (TLRs), and cytokines, and investigated the time course of an inflammatory response in rooster SCs triggered by exposure to the bacterial endotoxin lipopolysaccharide (LPS). SCs were isolated from 6-week-old chicken, cultured in vitro, and stimulated with 1 μg/ml LPS at different time courses (0, 6, 12, 24, and 48  h). Data on expression analysis revealed that all ten members of the chicken TLR family, nine members of the AvBD family, as well as eight cytokine genes were expressed in SCs. Quantitative real-time PCR analysis revealed that LPS treatment resulted in significant induction of the expression levels of six TLRs, six AvBDs, and four cytokine genes, while two cytokine genes were downregulated and two other genes were unchanged. The increasing interleukin 1β (IL1β) production was confirmed in the conditioned medium. Furthermore, the phagocytosis of SCs was increased after LPS treatment. In conclusion, these findings provide evidence that SCs express innate immune-related genes and respond directly to bacterial ligands. These genes represent an important component of the immune system, which could be integrated into semen, and present a distinctive constituent of the protective repertoire of the testis against ascending infections.
authors.affiliation: Laboratory of Physiology of Reproduction of Farm AnimalsDepartment of Animal Production, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, GreeceINRACNRS Université de Tours Haras Nationaux, Physiologie de la Reproduction et des Comportements, Nouzilly, France michageo@agro.auth.gr.
authors.forename: Georgios
authors.initials: G
authors.lastname: Michailidis
authors.affiliation: Laboratory of Physiology of Reproduction of Farm AnimalsDepartment of Animal Production, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, GreeceINRACNRS Université de Tours Haras Nationaux, Physiologie de la Reproduction et des Comportements, Nouzilly, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Anastasiadou
authors.affiliation: Laboratory of Physiology of Reproduction of Farm AnimalsDepartment of Animal Production, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, GreeceINRACNRS Université de Tours Haras Nationaux, Physiologie de la Reproduction et des Comportements, Nouzilly, France.
authors.forename: Edith
authors.initials: E
authors.lastname: Guibert
authors.affiliation: Laboratory of Physiology of Reproduction of Farm AnimalsDepartment of Animal Production, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, GreeceINRACNRS Université de Tours Haras Nationaux, Physiologie de la Reproduction et des Comportements, Nouzilly, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Froment
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D010587:Phagocytosis / Q000276:immunology; D012708:Sertoli Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D008070:Lipopolysaccharides