title: Study on variation of lipids during different growth phases of living cyanobacteria using easy ambient sonic-spray ionization mass spectrometry.
abstract: Lipids are important components of cell membranes and thylakoids in cyanobacteria, and they play vital roles in various biological activities. Real-time tracing of the variation of membrane lipids can provide insights of the physiological status of cyanobacterial cells. In this work, easy ambient sonic-spray ionization mass spectrometry (EASI-MS) was utilized to investigate the changes of acidic lipids in unicellular (Synechocystis 6803, Synechococcus 7002) and filamentous (Anabaena 7120) cyanobacteria during different growth phases. A sqdX mutant with a reduced synthesis of sulfoquinovosyl diacylglycerol (SQDG) was constructed to verify the acquired data of EASI-MS. Principal component analysis (PCA) was performed to compare the acquired data, enabling the discrimination of different species of cyanobacteria in day-to-day analysis. The results showed that the three representative cyanobacteria and their growth status can be easily determined on the basis of the lipids components detected by EASI-MS. Very interestingly, significant decreases of the ratios of SQDG/PG and dramatic changes of the unsaturation level of lipids were observed in different culture times in these cyanobacteria, and these two unique characters can be used describe the aging of cyanobacteria.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University , Beijing, 100871, P. R. China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Liu
authors.forename: Jialing
authors.initials: J
authors.lastname: Zhang
authors.forename: Honggang
authors.initials: H
authors.lastname: Nie
authors.forename: Chunxia
authors.initials: C
authors.lastname: Dong
authors.forename: Ze
authors.initials: Z
authors.lastname: Li
authors.forename: Zhenggao
authors.initials: Z
authors.lastname: Zheng
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.forename: Huwei
authors.initials: H
authors.lastname: Liu
authors.forename: Jindong
authors.initials: J
authors.lastname: Zhao
mesh_terms: D017033:Anabaena / Q000737:chemistry / Q000254:growth & development; D000458:Cyanobacteria / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism; D006017:Glycolipids / Q000032:analysis / Q000096:biosynthesis; D008055:Lipids / Q000032:analysis*; D008563:Membrane Lipids / Q000032:analysis / Q000235:genetics; D009154:Mutation; D010715:Phosphatidylglycerols / Q000032:analysis; D025341:Principal Component Analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D046939:Synechocystis / Q000737:chemistry / Q000235:genetics / Q000254:growth & development
chemical_list: D006017:Glycolipids; D008055:Lipids; D008563:Membrane Lipids; D010715:Phosphatidylglycerols; C013694:sulfoquinovosyl diglyceride