title: [Determining the tumor-cell density required for macroscopic observation of 5-ALA-induced fluorescence of protoporphyrin IX in cultured glioma cells and clinical cases].
abstract: We investigated the association between the cell density and intensity of 5-aminolevulinic acid-induced fluorescence of protoporphyrin IX in 3-dimensionally cultured C6 glioma cells. The ratio between 636-nm red fluorescence excited by a 405-nm laser and 513-nm green autofluorescence of the tissue was measured as the fluorescence intensity. A ratio exceeding 0.68 was macroscopically judged as fluorescence-positive by observers, and the cell density at this fluorescence intensity was 1×107< cells/mL. In clinical surgical fields, the fluorescence ratio was about 0.65 in cases judged as fluorescence-positive, similarly to that in cultured cells. Neurosurgeons and pathologists should recognize that tumor cells are present in fluorescence-negative regions. It is necessary to develop a device which measures fluorescence more simply than macroscopic observation for cutting-edge brain surgery.
authors.affiliation: Department of Neurosurgery, Faculty of Medical Sciences, University of Fukui.
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Kitai
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Norio
authors.initials: N
authors.lastname: Miyoshi
authors.forename: Bibin
authors.initials: B
authors.lastname: Andriana
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Neishi
authors.forename: Norichika
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Kikuta
mesh_terms: D000328:Adult; D000368:Aged; D000622:Aminolevulinic Acid / Q000494:pharmacology*; D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology* / Q000601:surgery; D002452:Cell Count; D045744:Cell Line, Tumor; D005260:Female; D005910:Glioma / Q000473:pathology* / Q000601:surgery; D006801:Humans; D008297:Male; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011524:Protoporphyrins / Q000494:pharmacology*; D051381:Rats
chemical_list: D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX