title: A golden gate modular cloning toolbox for plants.
abstract: Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.
authors.affiliation: Icon Genetics GmbH, Weinbergweg 22, Biozentrum Halle, 06120 Halle, Germany.
authors.forename: Carola
authors.initials: C
authors.lastname: Engler
authors.forename: Mark
authors.initials: M
authors.lastname: Youles
authors.forename: Ramona
authors.initials: R
authors.lastname: Gruetzner
authors.forename: Tim-Martin
authors.initials: TM
authors.lastname: Ehnert
authors.forename: Stefan
authors.initials: S
authors.lastname: Werner
authors.forename: Jonathan D G
authors.initials: JD
authors.lastname: Jones
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: Patron
authors.forename: Sylvestre
authors.initials: S
authors.lastname: Marillonnet
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D008957:Models, Genetic; D058615:Synthetic Biology / Q000379:methods*; D014026:Nicotiana / Q000235:genetics
keywords: Agrobacterium tumefaciens; gene assembly; genetic engineering; molecular cloning; plant biotechnology; vector construction