title: Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic.
abstract: Our aim in this study was to evaluate how the chemical stability of silver nanoparticles (SNs) influences their efficacy against Candida albicans and C. glabrata biofilms. Several parameters of SN stability were tested, namely, temperature (50ºC, 70ºC, and 100ºC), pH (5.0 and 9.0), and time of contact (5 h and 24 h) with biofilms. The control was defined as SNs without temperature treatment, pH 7, and 24 h of contact. These colloidal suspensions at 54 mg/L were used to treat mature Candida biofilms (48 h) formed on acrylic. Their efficacy was determined by total biomass and colony-forming unit quantification. Data were analyzed using analysis of variance and the Bonferroni post hoc test (α = 0.05). The temperature and pH variations of SNs did not affect their efficacy against the viable cells of Candida biofilms (P > 0.05). Moreover, the treatment periods were not decisive in terms of the susceptibility of Candida biofilms to SNs. These findings provide an important advantage of SNs that may be useful in the treatment of Candida-associated denture stomatitis.
authors.affiliation: Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Universidade Estadual Paulista, Araçatuba/São Paulo, Brazil douglasrmonteiro@hotmail.com.
authors.forename: Douglas Roberto
authors.initials: DR
authors.lastname: Monteiro
authors.affiliation: Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Universidade Estadual Paulista, Araçatuba/São Paulo, Brazil.
authors.forename: Aline Satie
authors.initials: AS
authors.lastname: Takamiya
authors.affiliation: Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Universidade Estadual Paulista, Araçatuba/São Paulo, Brazil.
authors.forename: Leonardo Perina
authors.initials: LP
authors.lastname: Feresin
authors.affiliation: LIEC-Department of Chemistry, Federal University of São Carlos, São Carlos/São Paulo, Brazil.
authors.forename: Luiz Fernando
authors.initials: LF
authors.lastname: Gorup
authors.affiliation: LIEC-Department of Chemistry, Federal University of São Carlos, São Carlos/São Paulo, Brazil.
authors.forename: Emerson Rodrigues
authors.initials: ER
authors.lastname: de Camargo
authors.affiliation: Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Universidade Estadual Paulista, Araçatuba/São Paulo, Brazil.
authors.forename: Alberto Carlos Botazzo
authors.initials: AC
authors.lastname: Delbem
authors.affiliation: Institute for Biotechnology and Bioengineering, Department of Biological Engineering, University of Minho, Braga, Portugal.
authors.forename: Mariana
authors.initials: M
authors.lastname: Henriques
authors.affiliation: Department of Dental Materials and Prosthodontics, Araçatuba Dental School, Universidade Estadual Paulista, Araçatuba/São Paulo, Brazil.
authors.forename: Debora Barros
authors.initials: DB
authors.lastname: Barbosa
mesh_terms: D000179:Acrylates; D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D018533:Biomass; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology; D041221:Candida glabrata / Q000187:drug effects* / Q000502:physiology; D015169:Colony Count, Microbial; D003778:Dentures / Q000382:microbiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles; D012834:Silver / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors
chemical_list: D000179:Acrylates; D000935:Antifungal Agents; D012834:Silver
keywords: Candida albicans; Candida glabrata; biofilms; chemical stability; silver nanoparticles