title: miR-137 regulates the migration of human umbilical vein endothelial cells by targeting ephrin-type A receptor 7.
abstract: MicroRNAs (miRNAs) are short non-coding RNAs, which negatively regulate gene expression. Post‑transcriptional regulation by miRNAs is important for organism development. In addition, endothelial cells are key regulators of angiogenesis. By using the 3-(4,5-dimethylthiazol-2-yl)‑2,5‑diphenyltetrazolium bromide (MTT), migration and gelatin sponge-chorioallantoic membrane assays, it was demonstrated that when miR-137 was overexpressed, cell viability and migration decreased. In addition, it was observed that blocking endogenous miR-137 increased cell viability and migration. Bioinformatics analysis indicated that the 3'‑untranslated region (3'UTR) of the ephrin type-A receptor 7 (EPHA7) has a putative binding site for miR-137. miR-137 is able to directly bind to the EPHA7 3'UTR and negatively regulate the expression of EPHA7. miR-137 is also able to decrease the growth and migration of human umbilical vein endothelial cells (HUVECs). The identification of the function of miR-137 and its target gene EPHA7 in HUVECs may provide novel insights into the mechanisms of angiogenesis.
authors.affiliation: Central Laboratory, Linyi People's Hospital, Shandong University, Linyi, Shandong 276000, P.R. China.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, Linyi People's Hospital, Shandong University, Linyi, Shandong 276000, P.R. China.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Heng
authors.affiliation: Department of Neurology, Linyi People's Hospital, Shandong University, Linyi, Shandong 276000, P.R. China.
authors.forename: Jixu
authors.initials: J
authors.lastname: Yu
authors.affiliation: Central Laboratory, Linyi People's Hospital, Shandong University, Linyi, Shandong 276000, P.R. China.
authors.forename: Quanping
authors.initials: Q
authors.lastname: Su
authors.affiliation: Central Laboratory, Linyi People's Hospital, Shandong University, Linyi, Shandong 276000, P.R. China.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Guan
authors.affiliation: Central Laboratory, Linyi People's Hospital, Shandong University, Linyi, Shandong 276000, P.R. China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: You
authors.affiliation: Central Laboratory, Linyi People's Hospital, Shandong University, Linyi, Shandong 276000, P.R. China.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: Central Laboratory, Linyi People's Hospital, Shandong University, Linyi, Shandong 276000, P.R. China.
authors.forename: Fengyuan
authors.initials: F
authors.lastname: Che
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D002465:Cell Movement; D002470:Cell Survival; D019295:Computational Biology; D005786:Gene Expression Regulation; D018390:Gene Targeting; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D018919:Neovascularization, Physiologic; D036141:Receptor, EphA7 / Q000235:genetics / Q000378:metabolism*; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D020413:3' Untranslated Regions; C545567:MIRN137 microRNA, human; D035683:MicroRNAs; D013778:Tetrazolium Salts; D013844:Thiazoles; D036141:Receptor, EphA7; C022616:thiazolyl blue