title: Silencing BMP-2 expression inhibits A549 and H460 cell proliferation and migration.
abstract: BACKGROUND
  Bone morphogenetic protein 2 (BMP-2) is a member of the TGF-β superfamily that is closely correlated with many malignancies, particularly lung cancer. However, the effects of silenced BMP-2 on lung cancer cell proliferation and migration are not clear.
  
  
  METHODS
  Using quantitative real-time RT-PCR, BMP-2 mRNA expression was detected in 61 non-small cell lung cancer (NSCLC) samples. Survival curves were generated using follow-up data. Relationships between clinical or pathological characteristics and prognosis were analyzed. Cell viability assays and transwell migration assays were used to evaluate the effects of BMP-2 silencing on cell proliferation and migration of A549 and H460 cells.
  
  
  RESULTS
  BMP-2 mRNA expression was higher in NSCLC tissues compared to matched adjacent normal tissues (P<0.01). High BMP-2 expression levels were significantly associated with the occurrence of lymph node metastases and tumor stage (P<0.05). There were significant differences in survival curves between groups with metastatic lymph nodes and non-metastatic lymph nodes, as well as between groups with low BMP-2 expression and groups with high BMP-2 expression. In addition, we observed decreased proliferation and migration rates of the NSCLC-derived cell lines A549 and H460 that were transfected with siBMP-2 (P<0.05).
  
  
  CONCLUSIONS
  BMP-2 mRNA is overexpressed in NSCLC samples and is a risk factor for survival in patients with NSCLC. BMP-2 silencing can significantly inhibit A549 and H460 cell proliferation and migration.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4263254471298866.
authors.forename: Heying
authors.initials: H
authors.lastname: Chu
authors.forename: Hailan
authors.initials: H
authors.lastname: Luo
authors.forename: Huaqi
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Bai
authors.forename: Furui
authors.initials: F
authors.lastname: Zhang
authors.forename: Ruirui
authors.initials: R
authors.lastname: Cheng
authors.forename: Shanshan
authors.initials: S
authors.lastname: Chen
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. zhaogq@zzu.edu.cn.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
authors.forename: Guojun
authors.initials: G
authors.lastname: Zhang
mesh_terms: D055396:Bone Morphogenetic Protein 2 / Q000235:genetics* / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D012307:Risk Factors; D013997:Time Factors; D014162:Transfection
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D012333:RNA, Messenger