title: Green tea catechin, epigallocatechin-3-gallate, attenuates the cell viability of human non-small-cell lung cancer A549 cells via reducing Bcl-xL expression.
abstract: Clinical and epidemiological studies have indicated that the consumption of green tea has a number of beneficial effects on health. Epigallocatechin-3-gallate (EGCg), the major polyphenolic compound present in green tea, has received much attention as an active ingredient. Among the numerous promising profiles of EGCg, the present study focused on the anticancer effects. Apoptosis induced by EGCg and subsequent cell growth suppression have been demonstrated in a number of cell culture studies. However, the underlying mechanism of apoptotic cell death remains unclear. Thus, the aim of the present study was to identify the major molecule that mediates proapoptotic cell death by EGCg. The effect of EGCg on cell proliferation and the induction of mRNA that modulates apoptotic cell death was evaluated in the A549 human non-small-cell lung cancer cell line. In addition, morphological changes were assessed by microscopy in A549 cells that had been treated with 100 μM EGCg for 24 h. The MTT assay revealed that cell proliferation was significantly reduced by EGCg in a dose-dependent manner (3-100 μM). The mRNA expression level of B-cell lymphoma-extra large (Bcl-xL) was decreased in A549 cells following 24 h incubation with 100 μM EGCg. Therefore, the results indicated that the inhibition of cell proliferation by EGCg may be achieved via suppressing the expression of the cell death-inhibiting gene, Bcl-xL.
authors.affiliation: First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, Nobeoka, Miyazaki 882-8508, Japan.
authors.forename: Jun-Ichiro
authors.initials: JI
authors.lastname: Sonoda
authors.affiliation: Department of Clinical Pharmacy and Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Sakuragaoka, Kagoshima 890-8520, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Ikeda
authors.affiliation: Department of Radiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Sakuragaoka, Kagoshima 890-8520, Japan.
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Baba
authors.affiliation: First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, Nobeoka, Miyazaki 882-8508, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Narumi
authors.affiliation: First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, Nobeoka, Miyazaki 882-8508, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Kawachi
authors.affiliation: First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, Nobeoka, Miyazaki 882-8508, Japan.
authors.forename: Erisa
authors.initials: E
authors.lastname: Tomishige
authors.affiliation: First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, Nobeoka, Miyazaki 882-8508, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nishihara
authors.affiliation: Department of Clinical Pharmacy and Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Sakuragaoka, Kagoshima 890-8520, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Takeda
authors.affiliation: Department of Clinical Pharmacology, Faculty of Pharmaceutical Science, Nagasaki International University, Sasebo, Nagasaki 859-3298, Japan.
authors.forename: Katsushi
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Clinical Biochemistry, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, Nobeoka, Miyazaki 882-8508, Japan.
authors.forename: Keizo
authors.initials: K
authors.lastname: Sato
authors.affiliation: First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, Nobeoka, Miyazaki 882-8508, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Motoya
keywords: A549 cells; B-cell lymphoma-extra large; apoptosis; epigallocatechin-3-gallate