title: Muscular tubes of urethra engineered from adipose-derived stem cells and polyglycolic acid mesh in a bioreactor.
abstract: We have explored the feasibility of using adipose-derived stem cells (ADSCs) and polyglycolic acid (PGA) for constructing muscular tubes of urethra in a bioreactor. With the induction of by 5-azacytidine, ADSCs were found to acquire a myoblast phenotype. Here we seeded ADSCs in a PGA mesh to construct the cell-PGA complex that was cultured statically for 1 week. Afterwards, the cell-PGA complex was subjected to extension stimulation in a bioreactor for 5 weeks. A muscular tube of urethra was formed after 6 weeks. Histological examination showed differentiated ADSCs and collagenous fibers had orientated well. This study demonstrates that tissue engineering of urethra tissues in vitro by using a bioreactor leads to tissue maturation and the differentiation of ADSCs. This novel technique could provide an effective approach for urethra tissue engineering.
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Yi Shan Road 600, Shanghai, 200233, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Ren-Yan
authors.initials: RY
authors.lastname: Zhao
authors.forename: Chen-Liang
authors.initials: CL
authors.lastname: Deng
mesh_terms: D019149:Bioreactors; D002454:Cell Differentiation; D032446:Myoblasts / Q000502:physiology*; D011100:Polyglycolic Acid / Q000378:metabolism*; D013234:Stem Cells / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D014521:Urethra / Q000502:physiology*
chemical_list: D011100:Polyglycolic Acid