title: Infections of nervous necrosis virus in wild and cage-reared marine fish from South China Sea with unexpected wide host ranges.
abstract: The concerns about the impact of the nervous necrosis virus (NNV) infections in wild fish have been raised. This paper presents the results of quarterly surveys of NNV in wild and cage-reared marine fish from South China Sea. Samples of 892 wild fish belonging to 69 species and 381 cage-reared fish belonging to 11 species were collected and were detected by seminested PCR and nested PCR. In the case of seminested PCR, the positive signal was detected in 3.0% and 3.1% samples of wild and cage-reared fish, respectively. However, by nested RT-PCR, the positive signal was observed in 42.3% and 63.0% samples of wild and cage-reared fish, respectively. If the fish species were considered, the positive signal was detected in 21.7% and 72.7% species of wild and cage-reared fish by seminested PCR assay, respectively. However, by nested RT-PCR, the positive signal was observed in 65.2% and 100% species of wild and cage-reared fish, respectively. The nucleotide sequences of the nested PCR products were determined. Phylogenetic tree showed that all the obtained viral isolates belonged to the red-spotted grouper nervous necrosis virus (RGNNV) genotype. Thirty-five species of the marine fish were the new hosts of NNV.
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China.
authors.forename: X D
authors.initials: XD
authors.lastname: Liu
authors.forename: J N
authors.initials: JN
authors.lastname: Huang
authors.forename: S P
authors.initials: SP
authors.lastname: Weng
authors.forename: X Q
authors.initials: XQ
authors.lastname: Hu
authors.forename: W J
authors.initials: WJ
authors.lastname: Chen
authors.forename: Z D
authors.initials: ZD
authors.lastname: Qin
authors.forename: X X
authors.initials: XX
authors.lastname: Dong
authors.forename: X L
authors.initials: XL
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: M
authors.initials: M
authors.lastname: Asim
authors.forename: W M
authors.initials: WM
authors.lastname: Wang
authors.forename: J G
authors.initials: JG
authors.lastname: He
authors.forename: L
authors.initials: L
authors.lastname: Lin
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D002681:China; D005393:Fish Diseases / Q000453:epidemiology* / Q000821:virology; D005398:Fisheries; D058507:Host Specificity; D054884:Host-Pathogen Interactions; D030381:Nodaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D009792:Oceans and Seas; D010802:Phylogeny; D012327:RNA Virus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D036022:Capsid Proteins
keywords: South China Sea; cage-reared fish; nervous necrosis virus; nested RT-PCR; seminested RT-PCR; wild fish