title: Mapping of the exchangeable and dispensable domains of the RNA 2-encoded 2A(HP) protein of arabis mosaic nepovirus.
abstract: The N-terminal domains of the RNA 2-encoded 2A(HP) proteins of the arabis mosaic (ArMV) and grapevine fanleaf (GFLV) nepoviruses were shown to be highly variable and a hotspot for intra- and inter-species recombination events. Chimeric ArMV-NW clones in which the N-terminal domain of 2A(HP) or the entire 2A(HP) of GFLV isolates replaced the corresponding domains of ArMV retained their infectivity, showing that the 2A(HP) proteins of ArMV-NW and GFLV are exchangeable. ArMN-NW clones with deletions of the N-terminal, core, or C-terminal domains of the ArMV-NW 2A(HP) were infectious in Chenopodium quinoa although viral RNA (especially RNA 2) accumulated at reduced levels. In contrast, deletion of the entire 2A(HP) protein or of the C-terminal two thirds of the protein abolished infectivity of the ArMV-NW clones. These results suggest that multiple functional domains are distributed throughout the 2A(HP) protein and are essential for the accumulation of viral RNA 2.
authors.affiliation: RLP Agroscience, AlPlanta - Institute for Plant Research, Breitenweg 71, 67435 Neustadt an der Weinstrasse, Germany.
authors.forename: Shaheen
authors.initials: S
authors.lastname: Nourinejhad Zarghani
authors.affiliation: RLP Agroscience, AlPlanta - Institute for Plant Research, Breitenweg 71, 67435 Neustadt an der Weinstrasse, Germany.
authors.forename: Laurence
authors.initials: L
authors.lastname: Dupuis-Maguiraga
authors.affiliation: RLP Agroscience, AlPlanta - Institute for Plant Research, Breitenweg 71, 67435 Neustadt an der Weinstrasse, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Bassler
authors.affiliation: RLP Agroscience, AlPlanta - Institute for Plant Research, Breitenweg 71, 67435 Neustadt an der Weinstrasse, Germany. Electronic address: thierry.wetzel@agroscience.rlp.de.
authors.forename: Thierry
authors.initials: T
authors.lastname: Wetzel
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics / Q000378:metabolism*; D017860:Nepovirus / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D012367:RNA, Viral / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
keywords: 2A(HP) protein; ArMV; GFLV; Nepovirus; Phylogeny; RNA 2; Replication; Secoviridae