title: Human memory T cells from the bone marrow are resting and maintain long-lasting systemic memory.
abstract: In the bone marrow, a population of memory T cells has been described that promotes efficient secondary immune responses and has been considered to be preactivated, owing to its expression of CD69 and CD25. Here we show that human bone marrow professional memory T cells are not activated but are resting in terms of proliferation, transcription, and mobility. They are in the G0 phase of the cell cycle, and their transcriptome is that of resting T cells. The repertoire of CD4(+) bone marrow memory T cells compared with CD4(+) memory T cells from the blood is significantly enriched for T cells specific for cytomegalovirus-pp65 (immunodominant protein), tetanus toxoid, measles, mumps, and rubella. It is not enriched for vaccinia virus and Candida albicans-MP65 (immunodominant protein), typical pathogens of skin and/or mucosa. CD4(+) memory T cells specific for measles are maintained nearly exclusively in the bone marrow. Thus, CD4(+) memory T cells from the bone marrow provide long-term memory for systemic pathogens.
authors.affiliation: Cell Biology.
authors.forename: Anna
authors.initials: A
authors.lastname: Okhrimenko
authors.affiliation: Bioinformatics.
authors.forename: Joachim R
authors.initials: JR
authors.lastname: Grün
authors.affiliation: Cell Biology.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Westendorf
authors.affiliation: Cell Biology,Signal Transduction, and.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Core Unit Cell Harvesting, Berlin-Brandenburg Center for Regenerative Therapies and.
authors.forename: Simon
authors.initials: S
authors.lastname: Reinke
authors.affiliation: Center for Musculoskeletal Surgery and.
authors.forename: Philipp
authors.initials: P
authors.lastname: von Roth
authors.affiliation: Center for Musculoskeletal Surgery and.
authors.forename: Georgi
authors.initials: G
authors.lastname: Wassilew
authors.affiliation: Department of Gastroenterology, Infectiology and Rheumatology, Charité University of Medicine Berlin, 12200 Berlin, Germany.
authors.forename: Anja A
authors.initials: AA
authors.lastname: Kühl
authors.affiliation: Clinical Tumor Immunology and Immunemonitoring, Institute for Medical Immunology, Charité University of Medicine Berlin, 13353 Berlin, Germany;
authors.forename: Robert
authors.initials: R
authors.lastname: Kudernatsch
authors.affiliation: Clinical Tumor Immunology and Immunemonitoring, Institute for Medical Immunology, Charité University of Medicine Berlin, 13353 Berlin, Germany;
authors.forename: Sonya
authors.initials: S
authors.lastname: Demski
authors.affiliation: Clinical Tumor Immunology and Immunemonitoring, Institute for Medical Immunology, Charité University of Medicine Berlin, 13353 Berlin, Germany;
authors.forename: Carmen
authors.initials: C
authors.lastname: Scheibenbogen
authors.affiliation: Osteoimmunology, Deutsches Rheuma-Forschungszentrum Berlin, 10117 Berlin, Germany;
authors.forename: Koji
authors.initials: K
authors.lastname: Tokoyoda
authors.affiliation: Cell Biology.
authors.forename: Mairi A
authors.initials: MA
authors.lastname: McGrath
authors.affiliation: Institute of Virology, Charité University of Medicine Berlin, 10117 Berlin, Germany; and.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Raftery
authors.affiliation: Institute of Virology, Charité University of Medicine Berlin, 10117 Berlin, Germany; and.
authors.forename: Günther
authors.initials: G
authors.lastname: Schönrich
authors.affiliation: Cell Biology.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Serra
authors.affiliation: Cell Biology.
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Chang
authors.affiliation: Cell Biology.
authors.forename: Andreas
authors.initials: A
authors.lastname: Radbruch
authors.affiliation: Cell Biology, dong@drfz.de.
authors.forename: Jun
authors.initials: J
authors.lastname: Dong
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D005260:Female; D006801:Humans; D007156:Immunologic Memory / Q000502:physiology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D037181:Lectins, C-Type / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016192:Resting Phase, Cell Cycle / Q000276:immunology*; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D000099192:CD69 Antigens; C508017:IL2RA protein, human
keywords: antigen-specific response; polyfunctional; short- and long-term memory