title: Monitoring of thermophilic adaptation of mesophilic anaerobe fermentation of sugar beet pressed pulp.
abstract: Anaerobe fermentation of sugar beet pressed pulp was investigated in pilot-scale digesters. Thermophilic adaptation of mesophilic culture was monitored using chemical analysis and metagenomic characterization of the sludge. Temperature adaptation was achieved by increasing the temperature gradually (2 °C day(-1)) and by greatly decreasing the OLR. During stable run, the OLR was increased gradually to 11.29 kg VS m(-3)d(-1) and biogas yield was 5% higher in the thermophilic reactor. VFA levels increased in the thermophilic reactor with increased OLR (acetic acid 646 mg L(-1), propionic acid 596 mg L(-1)), then VFA decreased and the operation was manageable beside the relative high tVFA (1300-2000 mg L(-1)). The effect of thermophilic adaptation on the microbial communities was studied using a sequencing-based metagenomic approach. Connections between physico-chemical parameters and populations of bacteria and methanogen archaea were revealed.
authors.affiliation: GázInnov Ltd., H-9400 Sopron, Asszonyvásár dűlő 31, Hungary.
authors.forename: Annamária
authors.initials: A
authors.lastname: Tukacs-Hájos
authors.affiliation: Seqomics Biotechnology Ltd., H-6782 Mórahalom, Vállalkozók útja 7., Hungary.
authors.forename: Bernadett
authors.initials: B
authors.lastname: Pap
authors.affiliation: Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62., Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Maróti
authors.affiliation: Biogáz Fejlesztő Ltd., H-7400 Kaposvár, Pécsi út 8-10., Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Szendefy
authors.affiliation: Institute of Chemistry, Faculty for Forestry, University of West Hungary, H-9400 Sopron, Bajcsy-Zs. u. 4., Hungary.
authors.forename: Piroska
authors.initials: P
authors.lastname: Szabó
authors.affiliation: Institute of Chemistry, Faculty for Forestry, University of West Hungary, H-9400 Sopron, Bajcsy-Zs. u. 4., Hungary. Electronic address: tretfalvi@emk.nyme.hu.
authors.forename: Tamás
authors.initials: T
authors.lastname: Rétfalvi
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D001421:Bacteria, Anaerobic / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D027461:Beta vulgaris / Q000737:chemistry / Q000378:metabolism*; D056804:Biofuels; D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D005285:Fermentation; D054892:Metagenome; D008697:Methane / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010865:Pilot Projects; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013696:Temperature
chemical_list: D056804:Biofuels; D008697:Methane
keywords: Metagenomic characterization; Pilot-scale; Sugar beet pressed pulp; Thermophilic adaptation