title: Neuroprotection against Aβ25-35-induced apoptosis by Salvia miltiorrhiza extract in SH-SY5Y cells.
abstract: The neurotoxicity of β-amyloid protein (Aβ) contributes significantly to the pathogenesis of Alzheimer's disease (AD), and hence the attractive therapeutic strategies focusing on the modulation of Aβ-induced neurotoxicity are warranted. The present study aims to investigate the neuroprotection and underlying mechanisms by which Salvia miltiorrhiza Bunge (Lamiaceae) extract (SME) protects against Aβ25-35-induced apoptosis in SH-SY5Y cells. 2h Pre-treatment of SH-SY5Y cells with SME (0.01, 0.1 or 0.2mg raw herb/ml) concentration-dependently attenuated Aβ25-35-induced cell death, as evidenced by the increase in cell viability and decrease in neuronal apoptosis. In addition, SME suppressed the increased intracellular reactive oxygen species levels, decreased the protein expression of cleaved caspase-3, cytosolic cytochrome c, and Bax/Bcl-2 ratio. These findings taken together suggest that SME provides substantial neuroprotection against Aβ25-35-induced neurotoxicity in SH-SY5Y cells, at least in part, via inhibiting oxidative stress and attenuating the mitochondria-dependent apoptotic pathway. The approach used in this study may also be useful for the screening of therapeutic agents for AD and other related neurodegenerative disease.
authors.affiliation: Department of Pathogenic Biology & Immunology, Medical College, Shenzhen University, Shenzhen, Guangdong 518060, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Yu
authors.affiliation: School of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Pharmacy, SZ Hospital of Traditional Chinese Medicine, Shenzhen, Guangdong 518020, PR China.
authors.forename: Hongzu
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Pathogenic Biology & Immunology, Medical College, Shenzhen University, Shenzhen, Guangdong 518060, PR China.
authors.forename: Sichang
authors.initials: S
authors.lastname: Qu
authors.affiliation: Department of Pathogenic Biology & Immunology, Medical College, Shenzhen University, Shenzhen, Guangdong 518060, PR China.
authors.forename: Xianghai
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Department of Pathogenic Biology & Immunology, Medical College, Shenzhen University, Shenzhen, Guangdong 518060, PR China.
authors.forename: Delong
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Department of Pathogenic Biology & Immunology, Medical College, Shenzhen University, Shenzhen, Guangdong 518060, PR China.
authors.forename: Yulian
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Pathogenic Biology & Immunology, Medical College, Shenzhen University, Shenzhen, Guangdong 518060, PR China.
authors.forename: Xinglin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pharmacy, SZ Hospital of Traditional Chinese Medicine, Shenzhen, Guangdong 518020, PR China. Electronic address: yuhm668@aliyun.com.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Liu
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D005434:Flow Cytometry; D006801:Humans; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010446:Peptide Fragments / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C074636:amyloid beta-protein (25-35)
keywords: Alzheimer’s disease; Aβ(25–35); Mitochondrial apoptotic pathway; Oxidative stress; SH-SY5Y cells; Salvia miltiorrhiza extract