title: Methicillin-resistant coagulase-negative staphylococci from healthy dogs in Nsukka, Nigeria.
abstract: The occurrence, resistance phenotype and molecular mechanisms of resistance of methicillin-resistant staphylococci from groin swabs of 109 clinically healthy dogs in Nsukka, Nigeria were investigated. The groin swab samples were cultured on mannitol salt agar supplemented with 10 μg of cloxacillin. Sixteen methicillin-resistant coagulase negative staphylococci (MRCoNS), all harbouring the mecA gene were isolated from 14 (12.8%) of the 109 dogs studied. The MRCoNS isolated were: S. sciuri subspecies rodentium, S. lentus, S. haemolyticus, and S. simulans with S. sciuri subspecies rodentium (62.5%) being the predominant species. Thirteen (81.3%) of the MRCoNS were resistant to tetracycline while 12 (75%) and 10 (62.5%) were resistant to kanamycin and trimthoprim-sulphamethoxazole respectively. None of the isolates was resistant to fusidic acid, linezolid and vancomycin. Thirteen (81.3%) of the MRCoNS were multi-drug resistance (MDR). Other antimicrobial genes detected were: blaZ, tet(K), tet(M), tet(L), erm(B), lnu(A), aacA-aphD, aphA3, str, dfr(G), cat pC221 , and cat pC223 . Methicillin-resistant staphylococci are common colonizers of healthy dogs in Nigeria with a major species detected being S. sciuri subsp. rodentium.
authors.affiliation: Department of Veterinary Pathology and Microbiology University of Nigeria Nsukka .
authors.forename: Kennedy F
authors.initials: KF
authors.lastname: Chah
authors.affiliation: Biochemistry and Molecular Biology University of La Rioja Logroño Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Gómez-Sanz
authors.affiliation: Department of Veterinary Public Health and Preventive Medicine University of Nigeria Nsukka .
authors.forename: John A
authors.initials: JA
authors.lastname: Nwanta
authors.affiliation: Department of Veterinary Public Health and Preventive Medicine University of Nigeria Nsukka .
authors.forename: Brendan
authors.initials: B
authors.lastname: Asadu
authors.affiliation: Department of Veterinary Pathology and Microbiology University of Nigeria Nsukka .
authors.forename: Ifeoma C
authors.initials: IC
authors.lastname: Agbo
authors.affiliation: Biochemistry and Molecular Biology University of La Rioja Logroño Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Lozano
authors.affiliation: Biochemistry and Molecular Biology University of La Rioja Logroño Spain.
authors.forename: Myriam
authors.initials: M
authors.lastname: Zarazaga
authors.affiliation: Biochemistry and Molecular Biology University of La Rioja Logroño Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Torres
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002353:Carrier State / Q000382:microbiology / Q000662:veterinary*; D003030:Coagulase / Q000172:deficiency*; D004285:Dogs; D005798:Genes, Bacterial; D006119:Groin / Q000382:microbiology; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D009549:Nigeria; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification* / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D003030:Coagulase
keywords: S. sciuri; dogs; methicillin-resistant; resistant genes