title: Allele-dependent association of chicken MHC class I molecules with the invariant chain.
abstract: Invariant chain (Ii) associates with MHC class I molecules in cross-presentation pathway in mouse, but the association of Ii with MHC class I molecules in chicken was not clear. In this study we selected five typical alleles from about 100 B-FA and some B-FB sequences and tested. Confocal microscopy revealed that only two alleles of α chain (CD type) rather than β chain showed incomplete co-localization with Ii own, or as a combined cytosolic and transmembrane domains in the co-transfected 293T cells, while other allele types, CK and CL, had no this ability. Co-immunoprecipitation (IP) indicated that one of both alleles bound only full-length Ii. Interestingly, further analysis of five sequences showed that the types CK had a tail and the most variable sites (39/44) localize within MHC I α1 and α2 regions, this suggests that the tail and multi-sites are crucial to the association with Ii. Additionally, qRT-PCR revealed that Ii transcription levels in different organs were positively correlated with those of B-FA or B-FB gene. These results suggest that the allele-dependent association of chicken MHC class I molecules with its Ii with bases on a multi-site determinative spatial structure and that Ii as carrier potentially perform similar roles in MHC II as well as in MHC I antigen peptide presentation.
authors.affiliation: Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, Changjiang West Road 130, Hefei 230036, China. Electronic address: fang7828887@126.com.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, Changjiang West Road 130, Hefei 230036, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Pan
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Jinzhai Road 96, Hefei 230026, China.
authors.forename: Jiegui
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, Changjiang West Road 130, Hefei 230036, China.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, Changjiang West Road 130, Hefei 230036, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, Changjiang West Road 130, Hefei 230036, China. Electronic address: yuwy@ahau.edu.cn.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Yu
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000944:Antigens, Differentiation, B-Lymphocyte / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D030161:Avian Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D002645:Chickens / Q000235:genetics* / Q000276:immunology*; D057809:HEK293 Cells; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D046912:Multiprotein Complexes / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D030161:Avian Proteins; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D018832:Molecular Chaperones; D046912:Multiprotein Complexes; D011994:Recombinant Proteins; C045084:invariant chain
keywords: Allele; Association; Chicken; Ii; MHC I