title: Practical fluorescence detection of acrolein in human plasma via a two-step tethering approach.
abstract: Acrolein, a cytotoxic α,β-unsaturated aldehyde and disease biomarker, was determined in plasma by means of a novel tethering strategy using Michael addition of the compound to a fluorescent dye, followed by immobilization of the product on microbeads via the aldehyde moiety. Elevation of blood acrolein was detected in mice treated with an anticancer agent cyclophosphamide, which releases acrolein upon activation. This method should be suitable for high-throughput diagnostic and clinical application.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Togashi
authors.forename: Takuya
authors.initials: T
authors.lastname: Terai
authors.forename: Hirotatsu
authors.initials: H
authors.lastname: Kojima
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.forename: Kazuei
authors.initials: K
authors.lastname: Igarashi
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Hirata
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
mesh_terms: D000171:Acrolein / Q000097:blood*; D000818:Animals; D000970:Antineoplastic Agents / Q000493:pharmacokinetics; D003520:Cyclophosphamide / Q000493:pharmacokinetics; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D012235:Rhodamines
chemical_list: C114557:5-carboxytetramethylrhodamine succinimidyl ester; D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D012235:Rhodamines; D000171:Acrolein; D003520:Cyclophosphamide