title: Moesin is a possible target molecule for cytomegalovirus-related Guillain-Barré syndrome.
abstract: OBJECTIVE
  Previous histochemical studies in the demyelinating form of Guillain-Barré syndrome (GBS), acute inflammatory demyelinating polyneuropathy (AIDP), have shown complement deposition on the surface of Schwann cells, and therefore unknown epitopes would be present on the outer surface of Schwann cells.
  
  
  METHODS
  We used a proteomic-based approach to search for the target molecules of AIDP in the extracted proteins from schwannoma cells. Sera were obtained from 40 patients with GBS, 31 controls with inflammatory disease, and 46 normal controls.
  
  
  RESULTS
  We found that patients with AIDP after cytomegalovirus (CMV) infection have serum autoantibodies against membrane-organizing extension spike protein (moesin), which is expressed in the Schwann cell processes at the nodes of Ranvier and is crucial for myelination. Of the 40 patients with GBS, 6 had recent CMV infection and 5 of them (83%) had high levels of serum immunoglobulin G antibodies against moesin. The anti-moesin antibodies were found in none of the control subjects with disease including 5 with CMV infection but no neuropathy, and only 2 (4%) of the 46 normal control subjects. Immunocytochemistry showed that moesin was stained at the distal tips of schwannoma cells by sera from the patients with CMV-related AIDP but not by sera from controls.
  
  
  CONCLUSIONS
  Moesin is a possible immunologic target molecule of pathogenic autoantibodies in patients with CMV-related AIDP.
  
  
  METHODS
  This study provides Class II evidence that levels of serum anti-moesin antibodies accurately distinguishes CMV-related AIDP from non-CMV-related AIDP (sensitivity 83%, specificity 93%).
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan. ssawai@faculty.chiba-u.jp.
authors.forename: Setsu
authors.initials: S
authors.lastname: Sawai
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Satoh
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Mori
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Sonoko
authors.initials: S
authors.lastname: Misawa
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Sogawa
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kazami
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Ishibashi
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Minako
authors.initials: M
authors.lastname: Beppu
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Kazumoto
authors.initials: K
authors.lastname: Shibuya
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ishige
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Sekiguchi
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Noda
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Sato
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Matsushita
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kodera
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Nomura
authors.affiliation: From the Departments of Molecular Diagnosis (S.S., M.S., K. Sogawa, T.K., M.I., M.B., T.I., K.N., K. Sato, K.M., F.N.) and Neurology (M.M., S.M., M.B., K. Shibuya, Y.S., S.K.), Graduate School of Medicine, Chiba University; and Department of Physics (Y.K.), School of Science, Kitasato University, Kanagawa, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kuwabara
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003586:Cytomegalovirus Infections / Q000150:complications / Q000188:drug therapy*; D004568:Electrodiagnosis; D004587:Electrophoresis, Agar Gel; D020275:Guillain-Barre Syndrome / Q000188:drug therapy* / Q000209:etiology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007150:Immunohistochemistry; D008840:Microfilament Proteins / Q000187:drug effects*; D009442:Neurilemmoma / Q000473:pathology; D040901:Proteomics; D011994:Recombinant Proteins / Q000032:analysis
chemical_list: D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008840:Microfilament Proteins; D011994:Recombinant Proteins; C071058:moesin