title: Voltammetric detection of S100B protein using His-tagged receptor domains for advanced glycation end products (RAGE) immobilized onto a gold electrode surface.
abstract: In this work we report on an electrochemical biosensor for the determination of the S100B protein. The His-tagged VC1 domains of Receptors for Advanced Glycation End (RAGE) products used as analytically active molecules were covalently immobilized on a monolayer of a thiol derivative of pentetic acid (DPTA) complex with Cu(II) deposited on a gold electrode surface. The recognition processes between the RAGE VC1 domain and the S100B protein results in changes in the redox activity of the DPTA-Cu(II) centres which were measured by Osteryoung square-wave voltammetry (OSWV). In order to verify whether the observed analytical signal originates from the recognition process between the His6-RAGE VC1 domains and the S100B protein, the electrode modified with the His6-RAGE C2 and His6-RAGE VC1 deleted domains which have no ability to bind S100B peptides were applied. The proposed biosensor was quite sensitive, with a detection limit of 0.52 pM recorded in the buffer solution. The presence of diluted human plasma and 10 nM Aβ(1-40) have no influence on the biosensor performance.
authors.affiliation: Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland. e.mikula@pan.olsztyn.pl.
authors.forename: Edyta
authors.initials: E
authors.lastname: Mikuła
authors.affiliation: Institute of Biochemistry and Biophysics of Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland. olawyslouch@ibb.waw.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Wysłouch-Cieszyńska
authors.affiliation: Institute of Biochemistry and Biophysics of Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland. lilia@ibb.waw.pl.
authors.forename: Liliya
authors.initials: L
authors.lastname: Zhukova
authors.affiliation: Institute of Biochemistry and Biophysics of Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland. monikapuchalska88@gmail.com.
authors.forename: Monika
authors.initials: M
authors.lastname: Puchalska
authors.affiliation: Chemistry Department, University of Leuven, Celestijnenlaan 200F, B-3001 Leuven, Belgium. peterverwilst@hotmail.com.
authors.forename: Peter
authors.initials: P
authors.lastname: Verwilst
authors.affiliation: Chemistry Department, University of Leuven, Celestijnenlaan 200F, B-3001 Leuven, Belgium. Wim.Dehaen@chem.kuleuven.be.
authors.forename: Wim
authors.initials: W
authors.lastname: Dehaen
authors.affiliation: Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland. j.radecki@pan.olsztyn.pl.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Radecki
authors.affiliation: Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland. h.radecka@pan.olsztyn.pl.
authors.forename: Hanna
authors.initials: H
authors.lastname: Radecka
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D025941:Protein Interaction Mapping / Q000295:instrumentation*; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000737:chemistry*; D064568:S100 Calcium Binding Protein beta Subunit / Q000032:analysis* / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D064568:S100 Calcium Binding Protein beta Subunit; C579160:S100B protein, human; D006639:Histidine; D006046:Gold