title: Position of the kissing-loop interaction associated with PTE-type 3'CITEs can affect enhancement of cap-independent translation.
abstract: The Panicum mosaic virus-like translation enhancer (PTE) functions as a cap-independent translation enhancer (3'CITE) in members of several Tombusviridae genera including 7/19 carmoviruses. For nearly all PTE, a kissing-loop connects the element with a hairpin found in several conserved locations in the genomic RNA (5' terminal hairpin or ~100 nt from the 5' end) and small subgenomic RNA (~63 nt from the 5' end). Moving the interaction closer to the 5' end in reporter mRNAs using Saguaro cactus virus (SCV) sequences had either a minimal or substantial negative effect on translation. Movement of the kissing loop from position 104 to the SCV 5' terminal hairpin also reduced translation by 4-fold. These results suggest that relocating the PTE kissing loop closer to the 5' end reduces PTE efficiency, in contrast to results for the Barley yellow dwarf BTE and Tomato bushy stunt virus Y-shaped 3'CITEs, suggesting that different 3'CITEs have different bridging requirements.
authors.affiliation: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
authors.forename: Maitreyi
authors.initials: M
authors.lastname: Chattopadhyay
authors.affiliation: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
authors.forename: Micki M
authors.initials: MM
authors.lastname: Kuhlmann
authors.affiliation: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
authors.forename: Kalyani
authors.initials: K
authors.lastname: Kumar
authors.affiliation: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA. Electronic address: simona@umd.edu.
authors.forename: Anne E
authors.initials: AE
authors.lastname: Simon
mesh_terms: D017360:Arabidopsis; D001483:Base Sequence; D019184:Carmovirus / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D017930:Genes, Reporter; D012315:RNA Caps; D046509:Tissue Culture Techniques; D019183:Tombusviridae / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012315:RNA Caps; D014764:Viral Proteins
keywords: Cap-independent translation; Carmovirus; Long-distance RNA:RNA interactions; RNA virus translation; Saguaro cactus virus