title: Distance effect of matrix attachment regions on transgene expression in stably transfected Chinese hamster ovary cells.
abstract: The β-globin matrix attachment regions (MARs) were inserted into the 5'-site of the eukaryotic expression vector cassette and DNA fragments 350 and 750 bp in length were inserted into the site to generate expression vectors with varying distances between the expression cassette and MAR. The vectors containing MARs increased chloramphenicol acetyltransferase (CAT) expression levels compared to the negative control vector lacking the MAR; the highest expression increase was 3.8-fold. A greater MAR-transgene distance (750 bp) correlated with a greater increase in transgene expression when compared to the control vector that lacked separation between the MAR and transgene. CAT gene copy numbers were higher in cells transformed with the vector possessing a smaller MAR-transgene distance (350 bp) than in cells belonging to the other three groups. However, MAR-induced transgene expression levels did not exhibit a direct relationship with gene copy number.
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, No. 601 Jinsui Road, Xinxiang, 453003, Henan Province, China.
authors.forename: Jun-He
authors.initials: JH
authors.lastname: Zhang
authors.forename: Xiao-Yin
authors.initials: XY
authors.lastname: Wang
authors.forename: Tian-Yun
authors.initials: TY
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Dong
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Chun-Peng
authors.initials: CP
authors.lastname: Zhao
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Chai
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D016466:CHO Cells; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics* / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D018628:Gene Dosage; D015870:Gene Expression; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D045170:Matrix Attachment Regions; D019076:Transgenes; D055544:beta-Globins / Q000235:genetics* / Q000378:metabolism
chemical_list: D055544:beta-Globins; D015500:Chloramphenicol O-Acetyltransferase