title: A primary assessment of the endophytic bacterial community in a xerophilous moss (Grimmia montana) using molecular method and cultivated isolates.
abstract: Investigating the endophytic bacterial community in special moss species is fundamental to understanding the microbial-plant interactions and discovering the bacteria with stresses tolerance. Thus, the community structure of endophytic bacteria in the xerophilous moss Grimmia montana were estimated using a 16S rDNA library and traditional cultivation methods. In total, 212 sequences derived from the 16S rDNA library were used to assess the bacterial diversity. Sequence alignment showed that the endophytes were assigned to 54 genera in 4 phyla (Proteobacteria, Firmicutes, Actinobacteria and Cytophaga/Flexibacter/Bacteroids). Of them, the dominant phyla were Proteobacteria (45.9%) and Firmicutes (27.6%), the most abundant genera included Acinetobacter, Aeromonas, Enterobacter, Leclercia, Microvirga, Pseudomonas, Rhizobium, Planococcus, Paenisporosarcina and Planomicrobium. In addition, a total of 14 species belonging to 8 genera in 3 phyla (Proteobacteria, Firmicutes, Actinobacteria) were isolated, Curtobacterium, Massilia, Pseudomonas and Sphingomonas were the dominant genera. Although some of the genera isolated were inconsistent with those detected by molecular method, both of two methods proved that many different endophytic bacteria coexist in G. montana. According to the potential functional analyses of these bacteria, some species are known to have possible beneficial effects on hosts, but whether this is the case in G. montana needs to be confirmed.
authors.affiliation: College of Life Science Capital Normal University Haidian DistrictBeijing China.
authors.forename: Xiao Lei
authors.initials: XL
authors.lastname: Liu
authors.affiliation: College of Life Science Capital Normal University Haidian DistrictBeijing China.
authors.forename: Su Lin
authors.initials: SL
authors.lastname: Liu
authors.affiliation: College of Life Science Capital Normal University Haidian DistrictBeijing China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Life Science Capital Normal University Haidian DistrictBeijing China.
authors.forename: Bi He
authors.initials: BH
authors.lastname: Kong
authors.affiliation: College of Life Science Capital Normal University Haidian DistrictBeijing China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Life Science Capital Normal University Haidian DistrictBeijing China.
authors.forename: Yan Hong
authors.initials: YH
authors.lastname: Li
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D058448:Biota; D019068:Bryopsida / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: bacterial diversity; cultivated isolates; endophytes; molecular method; moss