title: In vivo two-photon microscopy of the hippocampus using glass plugs.
abstract: Two-photon microscopy has been used in conjunction with micro-optics, such as GRIN lenses, to access subcortical structures in the intact mouse brain. In this study, we demonstrate the use of thick glass windows, or plugs, for high-resolution, large field-of-view two-photon imaging of the hippocampus in a live mouse. These plugs are less expensive, yield larger fields-of-view and are simpler to use than GRIN lenses while requiring less tissue removal compared to previous methods based on cortical ablation. To demonstrate the capabilities of our system, we show fluorescence images of dendritic spines in the CA1 region of the hippocampus in THY1-YFP transgenic mice.
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA.
authors.forename: Mary Grace M
authors.initials: MG
authors.lastname: Velasco
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Levene
keywords: (180.0180) Microscopy; (180.2520) Fluorescence microscopy; (180.4315) Nonlinear microscopy