title: Papain-induced experimental pulmonary emphysema in male and female mice.
abstract: In papain-induced models of emphysema, despite the existing extensive description of the cellular and molecular aspects therein involved, sexual hormones may play a complex and still not fully understood role. Hence, we aimed at exploring the putative gender-related differences in lung mechanics, histology and oxidative stress in papain-exposed mice. Thirty adult BALB/c mice received intratracheally either saline (50 μL) or papain (10 U/50 μL saline) once a week for 2 weeks. In males papain increased lung resistive and viscoelastic/inhomogeneous pressures, static elastance, and viscoelastic component of elastance, while females showed higher static elastance and resistive pressure only. Both genders presented similar higher parenchymal cellularity and mean alveolar diameter, and less collagen-elastic fiber content and body weight gain than their respective controls. Increased functional residual capacity was more prominent in males. Female papain-treated mice were more susceptible to oxidative stress. Thus, male and female papain-exposed mice respond differently, which should be carefully considered to avoid confounding results.
authors.affiliation: Laboratory of Respiration Physiology, Carlos Chagas Filho Institute of Biophysics, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Mariana Nascimento
authors.initials: MN
authors.lastname: Machado
authors.affiliation: Laboratory of Respiration Physiology, Carlos Chagas Filho Institute of Biophysics, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Silviane Fernandes da Silva
authors.initials: SF
authors.lastname: Figueirôa
authors.affiliation: Laboratory of Respiration Physiology, Carlos Chagas Filho Institute of Biophysics, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Flavia
authors.initials: F
authors.lastname: Mazzoli-Rocha
authors.affiliation: Laboratory of Integrative Histology, Institute of Biomedical Sciences, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Samuel dos Santos
authors.initials: Sdos S
authors.lastname: Valença
authors.affiliation: Laboratory of Respiration Physiology, Carlos Chagas Filho Institute of Biophysics, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: wazin@biof.ufrj.br.
authors.forename: Walter Araújo
authors.initials: WA
authors.lastname: Zin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008168:Lung / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000502:physiology; D010206:Papain; D011656:Pulmonary Emphysema / Q000473:pathology / Q000503:physiopathology*; D011897:Random Allocation; D015656:Respiratory Mechanics / Q000502:physiology*
chemical_list: D010206:Papain
keywords: Elastance; Histology; Lung mechanics; Oxidative stress; Papain; Pulmonary emphysema