title: Miniaturization of multiplexed planar recombinant antibody arrays for serum protein profiling.
abstract: BACKGROUND
  Antibody-based microarrays are a developing tool for high-throughput proteomics in health and disease. However, in order to enable global proteome profiling, novel miniaturized high-density antibody array formats must be developed.
  
  
  RESULTS
  In this proof-of-concept study, we have designed a miniaturized planar recombinant (single-chain Fragment variable). antibody array technology platform for multiplexed profiling of non-fractionated, directly labelled serum samples. The size of the individual spot features was reduced 225-times (78.5 μm(2)/spot) and the array density was increased 19-times (38,000 spots/cm(2)). These miniaturized, multiplexed arrays were produced, using a desktop nanofabrication system based on dip-pen nanolithography technology, and interfaced with a high-resolution fluorescent-based scanner. The reproducibility, sensitivity, specificity, and applicability of the set-up were demonstrated by profiling a set of well-characterized serum samples.
  
  
  CONCLUSIONS
  The designed antibody array platform opens up new possibilities for large-scale, multiplex profiling of crude proteomes in a miniaturized fashion.
authors.affiliation: Dept. of Immunotechnology & CREATE Health, Lund University, Medicon Village, SE-22381 Lund, Sweden.
authors.forename: Linn
authors.initials: L
authors.lastname: Petersson
authors.forename: Michael
authors.initials: M
authors.lastname: Coen
authors.forename: Nabil A
authors.initials: NA
authors.lastname: Amro
authors.forename: Lennart
authors.initials: L
authors.lastname: Truedsson
authors.forename: Carl A K
authors.initials: CA
authors.lastname: Borrebaeck
authors.forename: Christer
authors.initials: C
authors.lastname: Wingren
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008904:Miniaturization; D040081:Protein Array Analysis; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology
chemical_list: D000906:Antibodies; D001798:Blood Proteins; D011994:Recombinant Proteins