title: Characterization and subcellular localization of two 14-3-3 genes and their response to abiotic stress in wheat.
abstract: In order to investigate biological functions of the 14-3-3 genes and their response to abiotic stress, two cDNAs (designated as Ta14R1 and Ta14R2) encoding putative 14-3-3 proteins were isolated from wheat by PCR and rapid amplification of cDNA end (RACE) technique. The cDNA of Ta14R1 is 999bp and encodes a protein of 262 amino acids, while the cDNA of Ta14R2 is 897bp in length and encodes a protein of 261 amino acids. Transient expression assays using Ta14R1/Ta14R2-GFP fusion constructs indicated that Ta14R1 and Ta14R2 were located in cytoplasm and cell membrane but not in chloroplasts. Real-time quantitative (RT-PCR) analysis revealed that Ta14R1 and Ta14R2 were differentially expressed in wheat tissues and significantly up-regulated in roots and shoots 1d after germination, indicating they may play a role in process of seed germination. The expression of the two genes in roots and leaves were significantly induced by plant hormone ABA, as well as heat, cold and drought treatments, suggesting that the two 14-3-3 genes in wheat may be involved in ABA dependent stress-responding pathway and response to heat, cold and drought stress.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Meng
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Yaying
authors.initials: Y
authors.lastname: Wang
authors.forename: Ruixia
authors.initials: R
authors.lastname: Xiao
authors.forename: Hailun
authors.initials: H
authors.lastname: Liu
authors.forename: Xinguo
authors.initials: X
authors.lastname: Wang
authors.forename: Jiangping
authors.initials: J
authors.lastname: Ren
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Li
authors.forename: Hongbin
authors.initials: H
authors.lastname: Niu
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics*; D000040:Abscisic Acid / Q000494:pharmacology; D018076:DNA, Complementary; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018525:Germination; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological; D013696:Temperature; D014908:Triticum / Q000235:genetics / Q000502:physiology*
chemical_list: D048948:14-3-3 Proteins; D018076:DNA, Complementary; D000040:Abscisic Acid