title: Immunohistochemical comparison of biomarker expression in biopsy and surgical specimens of non-small cell lung cancer.
abstract: BACKGROUND
  Single tumors may show heterogeneity, and it is unclear whether biomarker expression in surgical and diagnostic biopsy samples correlates.
  
  
  METHODS
  We retrospectively identified lung cancer patients who were diagnosed by biopsy and underwent surgery between January 2007 and October 2010 at the Shimane University Hospital, Shimane, Japan. Thirty-two patients were identified. The expression of four predictive biomarkers was assessed, namely excision repair cross-complementing gene 1 (ERCC1), ribonucleotide diphosphate reductase M1 (RRM1), thymidylate synthase (TS), and class III beta-tubulin (BT). We also compared immunohistochemical staining in diagnostic biopsy and corresponding resected surgical samples.
  
  
  RESULTS
  Moderate correlation was seen between the expression of ERCC1, RRM1, TS, and BT in the biopsy and surgical specimens, with r values of 0.512 (p=0.003), 0.411 (p=0.020), 0.475 (p=0.006), and 0.404 (p=0.027), respectively.
  
  
  CONCLUSIONS
  Assessment of biopsy samples with immunohistochemical staining is a feasible and reliable method for use in clinical decision making.
authors.affiliation: Department of Internal Medicine, Division of Medical Oncology and Respiratory Medicine, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Tamio
authors.initials: T
authors.lastname: Okimoto
authors.affiliation: Department of Internal Medicine, Division of Medical Oncology and Respiratory Medicine, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Tsubata
authors.affiliation: Department of Internal Medicine, Division of Medical Oncology and Respiratory Medicine, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Akihisa
authors.initials: A
authors.lastname: Sutani
authors.affiliation: Department of Internal Medicine, Division of Medical Oncology and Respiratory Medicine, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fuchita
authors.affiliation: Department of Internal Medicine, Division of Medical Oncology and Respiratory Medicine, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Koba
authors.affiliation: Department of Internal Medicine, Division of Medical Oncology and Respiratory Medicine, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Hotta
authors.affiliation: Department of Internal Medicine, Division of Medical Oncology and Respiratory Medicine, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Hamaguchi
authors.affiliation: Department of Internal Medicine, Division of Medical Oncology and Respiratory Medicine, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Miura
authors.affiliation: Department of Internal Medicine, Division of Medical Oncology and Respiratory Medicine, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Shunichi
authors.initials: S
authors.lastname: Hamaguchi
authors.affiliation: Department of Internal Medicine, Division of Medical Oncology and Respiratory Medicine, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Ohe
authors.affiliation: Department of Respiratory Medicine, Shimane Prefectural Central Hospital, Shimane, Japan.
authors.forename: Takashige
authors.initials: T
authors.lastname: Kuraki
authors.affiliation: Laboratory of Surgical Pathology, Shimane University Hospital, Shimane, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Harada
authors.affiliation: Department of Organ Pathology, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Riruke
authors.initials: R
authors.lastname: Maruyama
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Miyamoto
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kishimoto
authors.affiliation: Department of Internal Medicine, Division of Medical Oncology and Respiratory Medicine, Shimane University Faculty of Medicine, Shimane, Japan isobeti@med.shimane-u.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Isobe
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018287:Carcinoma, Large Cell / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D004268:DNA-Binding Proteins / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012189:Retrospective Studies; D012262:Ribonucleoside Diphosphate Reductase; D013940:Thymidylate Synthase / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D014404:Tubulin; D025521:Tumor Suppressor Proteins; C475286:RRM1 protein, human; D012262:Ribonucleoside Diphosphate Reductase; D013940:Thymidylate Synthase; C071447:ERCC1 protein, human; D004720:Endonucleases
keywords: Biomarker; biopsy; immunohistochemistry; non-small-cell lung cancer; surgery