title: Stress-induced alterations in 5-HT1A receptor transcriptional modulators NUDR and Freud-1.
abstract: The effect of stress on the mRNA and protein level of the 5-HT1A receptor and two of its key transcriptional modulators, NUDR and Freud-1, was examined in the prefrontal cortex (PFC) and hippocampus (Hp) using rodent models: olfactory bulbectomy (OB) and prenatal stress (PS) in male and female rats; chronic mild stress in male rats (CMS) and pregnancy stress. In PFC, CMS induced the most widespread changes, with significant reduction in both mRNA and protein levels of NUDR, 5-HT1A receptor and in Freud-1 mRNA; while in Hp 5-HT1A receptor and Freud-1 protein levels were also decreased. In male, but not female OB rats PFC Freud-1 and 5-HT1A receptor protein levels were reduced, while in Hp 5-HT1A receptor, Freud-1 and NUDR mRNA's but not protein were reduced. In PS rats PFC 5-HT1A receptor protein was reduced more in females than males; while in Hp Freud-1 protein was increased in females. In pregnancy stress, PFC NUDR, Freud-1 and 5-HT1A protein receptor levels were reduced, and in HP 5-HT1A receptor protein levels were also reduced; in HP only NUDR and Freud-1 mRNA levels were reduced. Overall, CMS and stress during pregnancy produced the most salient changes in 5-HT1A receptor and transcription factor expression, suggesting a primary role for altered transcription factor expression in chronic regulation of 5-HT1A receptor expression. By contrast, OB (in males) and PS (in females) produced gender-specific reductions in PFC 5-HT1A receptor protein levels, suggesting a role for post-transcriptional regulation. These and previous data suggest that chronic stress might be a key regulator of NUDR/Freud-1 gene expression.
authors.affiliation: Department of Neurobiology,Institute of Pharmacology, Polish Academy of Sciences,Smetna 12, 31-343 Krakow,Poland.
authors.forename: Bernadeta
authors.initials: B
authors.lastname: Szewczyk
authors.affiliation: Department of Neurobiology,Institute of Pharmacology, Polish Academy of Sciences,Smetna 12, 31-343 Krakow,Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kotarska
authors.affiliation: Ottawa Hospital Research Institute (Neuroscience), University of Ottawa,451 Smyth Road, Ottawa, ON,Canada.
authors.forename: Mireille
authors.initials: M
authors.lastname: Daigle
authors.affiliation: Department of Neurobiology,Institute of Pharmacology, Polish Academy of Sciences,Smetna 12, 31-343 Krakow,Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Misztak
authors.affiliation: Department of Neurobiology,Institute of Pharmacology, Polish Academy of Sciences,Smetna 12, 31-343 Krakow,Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Sowa-Kucma
authors.affiliation: Department of Neurobiology,Institute of Pharmacology, Polish Academy of Sciences,Smetna 12, 31-343 Krakow,Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Rafalo
authors.affiliation: Department of Experimental Neuroendocrinology,Institute of Pharmacology, Polish Academy of Sciences,Smetna 12, PL 31-343 Krakow,Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Curzytek
authors.affiliation: Department of Experimental Neuroendocrinology,Institute of Pharmacology, Polish Academy of Sciences,Smetna 12, PL 31-343 Krakow,Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kubera
authors.affiliation: Department of Experimental Neuroendocrinology,Institute of Pharmacology, Polish Academy of Sciences,Smetna 12, PL 31-343 Krakow,Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Basta-Kaim
authors.affiliation: Department of Neurobiology,Institute of Pharmacology, Polish Academy of Sciences,Smetna 12, 31-343 Krakow,Poland.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Nowak
authors.affiliation: Ottawa Hospital Research Institute (Neuroscience), University of Ottawa,451 Smyth Road, Ottawa, ON,Canada.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Albert
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D009830:Olfactory Bulb / Q000601:surgery; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D044282:Receptor, Serotonin, 5-HT1A / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012737:Sex Factors; D013315:Stress, Psychological / Q000209:etiology / Q000473:pathology*; D014157:Transcription Factors
chemical_list: C476534:Cc2d1a protein, rat; C485478:Deaf1 protein, rat; D009687:Nuclear Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D044282:Receptor, Serotonin, 5-HT1A