title: Immunohistochemistry comparing endoscopic vein harvesting vs. open vein harvesting on saphenous vein endothelium.
abstract: OBJECTIVE
  The present study attempts to compare the immunohistochemistry (IHC) of von Willebrand factor (vWf) , endothelial cadherin, Caveolin and endothelial Nitric Oxide Synthase (eNOS) in VasoView Endoscopic Vein Harvesting (EVH) versus traditional Open Vein Harvesting (OVH) techniques for Coronary Artery Bypass Graft (CABG) Surgery performed in Javad al Aemeh Hospital of Mashhad, Iran in 2013,.
  
  
  METHODS
  Forty-seven patients were scheduled for CABG (30 EVH and 17 OVH) among whom patients with relatively same gender and similar age were selected. Three separate two cm vein samples were harvested from each patient's saphenous vein. Each portion was collected from distal, middle and proximal zones of the saphenous vein. The tissues were deparaffinized, and antigen retrieval was done using EZ-retriever followed by an immunohistochemistry evaluation with vWf, e-cadherin, Caveolin and eNOS. In addition, demographic questioner as of Lipid profile, FBS, BMI, and cardiovascular risk factors were collected. Data analyses, including parametric and nonparametric tests were undertaken using the SPSS 16 software. A P value < 0.05 was regarded as statistically significant.
  
  
  RESULTS
  The mean age of the EVH and OVH groups were 63.76 ± 9.51 and 63.63 ± 8.31 years respectively with no significant difference between them (p = 0.989). In addition, there was no great difference between the EVH and OVH groups in lipid profile, DM, HTN, smoking history, CVA, and valvular dysfunction (P > 0.05). Qualitative report of vWf, e-cadherin, Caveolin and eNOS reveals no significant difference between the EVH and OVH (P > 0.05).
  
  
  CONCLUSIONS
  This study indicates that VasoView EVH technique causes no endothelial damage in comparison with OVH. This study could be a molecular confirmation for the innocuous of EVH technique.
authors.forename: Mohammad Hassan
authors.initials: MH
authors.lastname: Nezafati
authors.affiliation: Student Research Committee, Mashhad University of Medical Sciences, No 124,, Niloufar 8th , Sadjad Blvd, Mashhad 91878, Iran. pouya_dg@yahoo.com.
authors.forename: Pouya
authors.initials: P
authors.lastname: Nezafati
authors.forename: Sakineh
authors.initials: S
authors.lastname: Amoueian
authors.forename: Armin
authors.initials: A
authors.lastname: Attaranzadeh
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Rahimi
mesh_terms: D000368:Aged; D015820:Cadherins / Q000378:metabolism; D022461:Caveolins / Q000378:metabolism; D001026:Coronary Artery Bypass; D003324:Coronary Artery Disease / Q000378:metabolism / Q000601:surgery*; D004724:Endoscopy; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D011446:Prospective Studies; D012501:Saphenous Vein / Q000378:metabolism* / Q000637:transplantation; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D015820:Cadherins; D022461:Caveolins; D014841:von Willebrand Factor; D052250:Nitric Oxide Synthase Type III