title: Morphology and ciliary motion of mucosa in the Eustachian tube of neonatal and adult gerbils.
abstract: The Eustachian tube is a small canal that connects the tympanic cavity with the nasal part of the pharynx. The epithelial lining of the Eustachian tube contains a ciliated columnar epithelium at the tympanic cavity and a pseudostratified, ciliated columnar epithelium with goblet cells near the pharynx. The tube serves to equalize air pressure across the eardrum and drains mucus away from the middle ear into the nasopharynx. Blockage of the Eustachian tube is the most common cause of all forms of otitis media, which is common in children. In the present study, we examined the epithelial lining of the Eustachian tube in neonatal and adult gerbils, with a focus on the morphological and functional development of ciliated cells in the mucosa. The length of the tube is ∼8.8 mm in adult gerbils. Scanning electron microscopy showed that the mucosal member near the pharyngeal side contains a higher density of ciliated cells and goblet cells than that near the tympanic side. The cilia beat frequency is 11 Hz. During development, the length of the Eustachian tube increased significantly between postnatal day 1 (P1) and P18. Scanning electron microscopy showed that the mucosa contained a high density of ciliated cells with a few goblet cells at P1. The density of ciliated cells decreased while the density of goblet cells increased during development. At P18, the mucosa appeared to be adult-like. Interestingly, the ciliary beat frequency measured from ciliated cells at P1 was not statistically different from that measured from adult animals. Our study suggests that the Eustachian tube undergoes significant anatomical and histological changes between P1 and P18. The tube is morphologically and functionally mature at P18, when the auditory function (sensitivity and frequency selectivity) is mature in this species.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, P.R. China; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, Nebraska, United States of America.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, Nebraska, United States of America.
authors.forename: Huizhan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology, Hospital of Zhongshan Qingpu, Fudan University, Shanghai, P.R. China; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, Nebraska, United States of America.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, Nebraska, United States of America.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, P.R. China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Gong
authors.affiliation: Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, Nebraska, United States of America.
authors.forename: David
authors.initials: D
authors.lastname: He
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D002923:Cilia / Q000502:physiology* / Q000648:ultrastructure; D004432:Ear, Middle / Q000166:cytology / Q000254:growth & development / Q000648:ultrastructure; D004847:Epithelial Cells / Q000166:cytology / Q000648:ultrastructure; D005064:Eustachian Tube / Q000166:cytology / Q000254:growth & development* / Q000648:ultrastructure; D005849:Gerbillinae / Q000033:anatomy & histology / Q000254:growth & development; D020397:Goblet Cells / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009038:Motion; D009831:Olfactory Mucosa / Q000166:cytology / Q000254:growth & development* / Q000648:ultrastructure; D014432:Tympanic Membrane / Q000166:cytology / Q000254:growth & development / Q000648:ultrastructure