title: Methods of split reporter reconstitution for the analysis of biomolecules.
abstract: Intracellular signaling inside living cells is controlled by the specific localization of biomolecules, including proteins, with timescales ranging from milliseconds to several hours. To elucidate the related spatial and temporal signal processes, development of optical probes for cellular events is a challenging task in present studies. Herein, we describe recent advances in the basic design of the optical probes, which have been inspired by luminescent creatures, and their practical application to visualize intracellular events in living cells and animals. A discussion of different probe designs reveals their benefits and shortcomings.
authors.affiliation: Department of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. ozawa@chem.s.u-tokyo.ac.jp.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yoshimura
authors.forename: Takeaki
authors.initials: T
authors.lastname: Ozawa
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051379:Mice; D060066:Protein Interaction Maps; D011506:Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D014982:Xenopus laevis / Q000254:growth & development
chemical_list: D004798:Enzymes; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D012313:RNA
keywords: biosensors; fluorescent probes; luminescence; protein-protein interactions; split reporters