title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis.
abstract: The yeast strains Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis were isolated from soil samples and identified as oleaginous yeast strains beneficial for the establishment of microbial production processes for sustainable lipid production suitable for several industrial applications. When cultured in bioreactors with glucose as the sole carbon source C. podzolicus yielded 31.8% lipid per dry biomass at 20°C, while T. porosum yielded 34.1% at 25°C and P. segobiensis 24.6% at 25°C. These amounts correspond to lipid concentrations of 17.97 g/L, 17.02 g/L and 12.7 g/L and volumetric productivities of 0.09 g/Lh, 0.1 g/Lh and 0.07 g/Lh, respectively. During the culture of C. podzolicus 30 g/l gluconic acid was detected as by-product in the culture broth and 12 g/L gluconic acid in T. porosum culture. The production of gluconic acid was eliminated for both strains when glucose was substituted by xylose as the carbon source. Using xylose lipid yields were 11.1 g/L and 13.9 g/L, corresponding to 26.8% and 33.4% lipid per dry biomass and a volumetric productivity of 0.07 g/Lh and 0.09 g/Lh, for C. podzolicus and T. porosum respectively. The fatty acid profile analysis showed that oleic acid was the main component (39.6 to 59.4%) in all three strains and could be applicable for biodiesel production. Palmitic acid (18.4 to 21.1%) and linolenic acid (7.5 to 18.7%) are valuable for cosmetic applications. P. segobiensis had a considerable amount of palmitoleic acid (16% content) and may be suitable for medical applications.
authors.affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Engler-Bunte-Ring 1, Karlsruhe, 76131, Germany.
authors.forename: Ines
authors.initials: I
authors.lastname: Schulze
authors.affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Engler-Bunte-Ring 1, Karlsruhe, 76131, Germany.
authors.forename: Silla
authors.initials: S
authors.lastname: Hansen
authors.affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Engler-Bunte-Ring 1, Karlsruhe, 76131, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Großhans
authors.affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Engler-Bunte-Ring 1, Karlsruhe, 76131, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Rudszuck
authors.affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Engler-Bunte-Ring 1, Karlsruhe, 76131, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Ochsenreither
authors.affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Engler-Bunte-Ring 1, Karlsruhe, 76131, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Syldatk
authors.affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Engler-Bunte-Ring 1, Karlsruhe, 76131, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Neumann
keywords: Cryptococcus podzolicus; Microbial lipid production; Oleaginous yeasts; Pichia segobiensis; Screening; Trichosporon porosum