title: Kinetics of sucrose conversion to fructo-oligosaccharides using enzyme (invertase) under free condition.
abstract: The study reports the synthesis of fructo-oligosaccharide (FOS) from sucrose using invertase derived from Saccharomyces cerevisiae. The reaction was conducted in a batch mode under free enzyme condition. Fructo-oligosaccharide formation was detected at a high sucrose concentration of over 200 g/L. The investigation was extended to study the effect of different parameters such as initial sucrose concentration (ISC), pH, and enzyme concentration. A maximum FOS yield of 10 % (dry basis) was observed using 525 g/L of ISC, with 6 U/mL of the enzyme, and pH 5.5 at 40 °C. 1-Kestose was the major product of among different forms of FOS. The FOS yield increased with an increase in sucrose concentration up to 525 g/L, beyond which it started to decrease. However, the maximum FOS yield was not affected by the increasing concentration of the enzyme beyond a certain level (2 U/mL). Furthermore, the activity of enzyme slightly increased with an increase in the pH up to 6, and thereafter it declined. Addition of glucose decreased the FOS yield because of enzyme inhibition. A five-step, ten-parameter model was developed, for which the simulation was performed in COPASI. The results predicted by the model were consistent with the experimental data.
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Kanpur, Kanpur, 208016, India.
authors.forename: Deepa C
authors.initials: DC
authors.lastname: Khandekar
authors.forename: Tapas
authors.initials: T
authors.lastname: Palai
authors.forename: Aman
authors.initials: A
authors.lastname: Agarwal
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Bhattacharya
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003198:Computer Simulation; D005632:Fructose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009844:Oligosaccharides / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012984:Software; D013395:Sucrose / Q000737:chemistry*; D013696:Temperature; D014312:Trisaccharides / Q000737:chemistry; D043324:beta-Fructofuranosidase / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D014312:Trisaccharides; C072433:1-kestose; D005632:Fructose; D013395:Sucrose; D043324:beta-Fructofuranosidase; D005947:Glucose