title: Immunoregulation effects of bone marrow-derived mesenchymal stem cells in xenogeneic acellular nerve grafts transplant.
abstract: This study evaluated whether bone marrow-derived mesenchymal stem cells (BM-MSCs) combined with xenogeneic acellular nerve grafts (xANGs) would reduce the inflammation reaction of xANGs transplantation. BM-MSCs were extracted, separated, purified, and cultured from the bone marrow of rats. Then BM-MSCs were seeded into 5 mm xANGs as experimental group, while xANGs group was chosen as control. Subcutaneous implantation and nerve grafts transplantation were done in this study. Walking-track tests, electrophysiological tests, H&E staining, and immunostaining of CD4, CD8, and CD68 of subcutaneous implantations, cytokine concentrations of IL-2, IL-10, IFN-γ and TNF-α in lymphocytes supernatants and serum of the two groups were evaluated. Walking-track tests and electrophysiological tests suggested the group of BM-MSCs with xANGs obtained better results than xANGs group (P < 0.05). H&E staining and immunostaining of CD4, CD8, and CD68 of subcutaneous implantations showed there were less inflammatory cells in the group of BM-MSCs when compared with the xANGs group. The cytokine concentrations of IL-2, IFN-γ, and TNF-α in BM-MSCs group were lower than xANGs group in lymphocytes supernatants and serum (P < 0.05). However, IL-10 concentrations in BM-MSCs group were higher than xANGs group (P < 0.05). xANG with BM-MSCs showed better nerve repair function when compared with xANG group. Furthermore, xANG with BM-MSCs showed less inflammatory reaction which might indicate the reason of its better nerve regeneration.
authors.affiliation: The First Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
authors.forename: Zefeng
authors.initials: Z
authors.lastname: Yu
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Dang
authors.forename: Kunzheng
authors.initials: K
authors.lastname: Wang
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D015704:CD4 Antigens / Q000378:metabolism; D002454:Cell Differentiation; D016207:Cytokines / Q000097:blood; D008214:Lymphocytes / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology*; D009417:Nerve Tissue / Q000637:transplantation*; D010006:Osteoblasts / Q000166:cytology; D019919:Prosthesis Implantation; D017207:Rats, Sprague-Dawley; D040521:Subcutaneous Tissue / Q000473:pathology; D034421:Sus scrofa; D014183:Transplantation, Heterologous; D016138:Walking; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015704:CD4 Antigens; D016207:Cytokines; C067980:CD68 antigen, human; D000097382:CD68 Molecule