title: In vivo tumor vasculature targeted PET/NIRF imaging with TRC105(Fab)-conjugated, dual-labeled mesoporous silica nanoparticles.
abstract: Multifunctional mesoporous silica nanoparticles (MSN) with well-integrated multimodality imaging properties have generated increasing research interest in the past decade. However, limited progress has been made in developing MSN-based multimodality imaging agents to image tumors. We describe the successful conjugation of, copper-64 ((64)Cu, t1/2 = 12.7 h), 800CW (a near-infrared fluorescence [NIRF] dye), and TRC105 (a human/murine chimeric IgG1 monoclonal antibody) to the surface of MSN via well-developed surface engineering procedures, resulting in a dual-labeled MSN for in vivo targeted positron emission tomography (PET) imaging/NIRF imaging of the tumor vasculature. Pharmacokinetics and tumor targeting efficacy/specificity in 4T1 murine breast tumor-bearing mice were thoroughly investigated through various in vitro, in vivo, and ex vivo experiments. Dual-labeled MSN is an attractive candidate for future cancer theranostics.
authors.affiliation: Department of Radiology, University of Wisconsin-Madison , Madison, Wisconsin 53792, United States.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Tapas R
authors.initials: TR
authors.lastname: Nayak
authors.forename: Shreya
authors.initials: S
authors.lastname: Goel
authors.forename: Hector F
authors.initials: HF
authors.lastname: Valdovinos
authors.forename: Hao
authors.initials: H
authors.lastname: Hong
authors.forename: Charles P
authors.initials: CP
authors.lastname: Theuer
authors.forename: Todd E
authors.initials: TE
authors.lastname: Barnhart
authors.forename: Weibo
authors.initials: W
authors.lastname: Cai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000493:pharmacokinetics; D003301:Copper Radioisotopes / Q000493:pharmacokinetics; D005260:Female; D005453:Fluorescence; D007074:Immunoglobulin G / Q000276:immunology; D015674:Mammary Neoplasms, Animal / Q000000981:diagnostic imaging* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D064847:Multimodal Imaging; D053758:Nanoparticles / Q000737:chemistry*; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging*; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D012822:Silicon Dioxide / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D003301:Copper Radioisotopes; D007074:Immunoglobulin G; D019275:Radiopharmaceuticals; D012822:Silicon Dioxide; C579557:carotuximab
keywords: mesoporous silica nanoparticle (MSN); near-infrared fluorescence (NIRF) imaging; positron emission tomography (PET) imaging; vasculature targeting