title: Development and evaluation of the rVP-ELISA for detection of antibodies against porcine parvovirus.
abstract: The gene encoding the VP2 protein of porcine parvovirus (PPV) was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination (HA), Western blotting using PPV positive sera. The purified rVP2 proteins were used as coating antigen to establish a rVP-ELISA method for detection of PPV positive and negative sera from pigs. The optimal operating conditions of the rVP-ELISA were: the concentration of rVP2 proteins coated on the wells was 2 μg/mL; the diluted concentration of serum was 1: 150 and that of the enzyme-labeled antibody was 1: 6000. A total of 596 sera were detected by this assay, and the average positive rate was 87%. Compared with France LSI kit, the result showed that the coincidence rate was 96.7%. In conclusion, the rVP2-ELISA is a sensitive and specific method for detecting antibodies against PPV.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100094, China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; College of Animal Science and Technology, HLJ August First Land Reclamation University, Daqing 163319, China.
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; College of Animal Science and Technology, HLJ August First Land Reclamation University, Daqing 163319, China.
authors.forename: Tiecheng
authors.initials: T
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100094, China; College of Animal Science and Technology, HLJ August First Land Reclamation University, Daqing 163319, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100094, China.
authors.forename: Yakun
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Nanyang Normal University, 1638 Wolong Road, Nanyang 473061, China.
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100094, China; College of Animal Science and Technology, HLJ August First Land Reclamation University, Daqing 163319, China. Electronic address: cuishangjin@126.com.
authors.forename: Shangjin
authors.initials: S
authors.lastname: Cui
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D019411:Clinical Laboratory Techniques / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005822:Genetic Vectors; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D028323:Parvovirus, Porcine / Q000276:immunology* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D061987:Sf9 Cells; D018411:Spodoptera; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C528774:VP2 protein, porcine parvovirus
keywords: Insect-baculovirus system; Porcine parvovirus; rVP-ELISA; rVP2