title: Association of coagulase-negative staphylococcal species, mammary quarter milk somatic cell count, and persistence of intramammary infection in dairy cattle.
abstract: This study was conducted to evaluate the association between subclinical intramammary infection (IMI) with coagulase-negative staphylococci (CNS), mammary quarter milk somatic cell count (SCC), and persistence of IMI in dairy cattle. Convenience samples of CNS isolates harvested from milk samples of subclinically infected mammary quarters collected between 4 and 2wk before drying-off, between 2wk before drying-off and the day of drying-off, within 24h after calving, between 1 and 2wk after calving, and during lactation were evaluated. Isolates were obtained from the Canadian Bovine Mastitis Research Network culture bank and were identified to the species level using rpoB gene sequencing. Cow and quarter-level data were obtained from the Canadian Bovine Mastitis Research Network database and used for statistical analyses. In addition, for mammary quarters that had more than one isolation of the same CNS species at different time points, the isolates were evaluated using pulsed-field gel electrophoresis to identify persistent IMI. Milk SCC was compared between mammary quarters infected with different CNS species and to a cohort of uninfected mammary quarters. A total of 877 isolates from 643 mammary quarters of 555 cows on 89 Canadian dairy farms were identified to the species level. Twenty different species were identified, with Staphylococcus chromogenes being the most common species identified (48% of isolates), followed by Staphylococcus simulans (19%) and Staphylococcus xylosus (10%). Of the 20 species identified, only 9 species were found in persistently infected quarters. Milk SCC was significantly higher in the CNS-infected mammary quarters than in the uninfected control quarters for 8 of the 20 species studied. Also, mean SCC differed significantly between mammary quarters infected with different CNS species. Within a given species, a high degree of variability was noted in milk SCC. These data corroborate recent data from Europe with regard to the predominance of certain species of CNS (e.g., Staph. chromogenes). In addition, some species of CNS appear to have a greater effect on milk SCC. Finally, some CNS species are associated with persistent IMI suggesting that some species (e.g., Staph. chromogenes and Staph. simulans) are better host-adapted, whereas others may have an environmental reservoir.
authors.affiliation: Department of Veterinary Medicine and Surgery, University of Missouri, Columbia 65211.
authors.forename: P R
authors.initials: PR
authors.lastname: Fry
authors.affiliation: Department of Veterinary Medicine and Surgery, University of Missouri, Columbia 65211. Electronic address: middletonjr@missouri.edu.
authors.forename: J R
authors.initials: JR
authors.lastname: Middleton
authors.affiliation: Department of Pathology and Microbiology, University of Montreal, Quebec, Canada J2S 7C6.
authors.forename: S
authors.initials: S
authors.lastname: Dufour
authors.affiliation: Production Animal Health Centre, University of Adelaide, Australia 5371.
authors.forename: J
authors.initials: J
authors.lastname: Perry
authors.affiliation: Department of Pathology and Microbiology, University of Montreal, Quebec, Canada J2S 7C6.
authors.forename: D
authors.initials: D
authors.lastname: Scholl
authors.affiliation: Department of Health Management, University of Prince Edward Island, Charlottetown CIA 4P3, Canada.
authors.forename: I
authors.initials: I
authors.lastname: Dohoo
mesh_terms: D000818:Animals; D002170:Canada; D002417:Cattle; D002452:Cell Count / Q000662:veterinary*; D003030:Coagulase / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D007774:Lactation; D008414:Mastitis, Bovine / Q000382:microbiology; D008892:Milk / Q000166:cytology* / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology* / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification*
chemical_list: D003030:Coagulase
keywords: bovine; coagulase-negative staphylococcus; mastitis