title: Deposition of superparamagnetic nanohydroxyapatite on iron-fibrin substrates: preparation, characterization, cytocompatibility and bioactivity studies.
abstract: In the present study, nanosized hydroxyapatite (nHAp) was formed on iron-fibrin substrates and its physico-chemical properties were characterized. The prepared iron-fibrin-nanohydroxyapatite (IF-nHAp) composite was needle shaped with an average width of about 30nm and length of 80nm. The vibrating sample magnetometer (VSM) was used to evaluate the superparamagnetic behavior of the nanocomposite, IF-nHAp. Hemolysis and ELISA (enzyme linked immunosorbent assay) were performed to evaluate the its bio/immunocompatibility and MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay using osteoblast cells was performed to scrutinize its proliferative potential. Alkaline phosphatase activity (ALP) and calcium deposition were studied to investigate the osteogenic property of the nanocomposite. RT-PCR (real time-polymerase chain reaction) was used to quantify the mRNA levels of ALP, OC (osteocalcin), and OPN (osteopontin) genes involved in the osteogenic differentiation and matrix mineralization. Further, the bone bonding ability of IF-nHAp was observed by the deposition of apatite layers on the composite incubated in simulated body fluid (SBF).
authors.affiliation: Bio-Products Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, India.
authors.forename: Weslen S
authors.initials: WS
authors.lastname: Vedakumari
authors.affiliation: PES Institute of Technology, Banashankari Stage III, Bangalore 560 085, India.
authors.forename: Vishnu M
authors.initials: VM
authors.lastname: Priya
authors.affiliation: Bio-Products Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, India. Electronic address: sastrytp@hotmail.com.
authors.forename: Thotapalli P
authors.initials: TP
authors.lastname: Sastry
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology*; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000138:chemical synthesis* / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005337:Fibrin / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007501:Iron / Q000494:pharmacology*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D060306:Magnetometry; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling
chemical_list: D001672:Biocompatible Materials; D058185:Magnetite Nanoparticles; D005337:Fibrin; D017886:Durapatite; D007501:Iron; D000469:Alkaline Phosphatase
keywords: Immunocompatibility; Iron–fibrin; Nanohydroxyapatite; Osteogenesis; RT-PCR