title: Discriminability measures and time-frequency features: an application to vibrissal tactile discrimination.
abstract: BACKGROUND
  Often, the first problem that the neuroscientist must face is to determine if a specific stimulus set applied to biological system produces specific, precise and well differentiated responses.
  
  
  METHODS
  In the present study we have proposed four discriminability measures to evaluate the feasibility of differentiating experimental conditions: information measures based on information theory, percentage overlap based on Linacre method, Bhattacharyya distance and univariate standard distance. All discriminability measures were evaluated on experimental protocols related to vibrissal tactile discrimination.
  
  
  RESULTS
  Time-frequency features were extracted from afferent discharges and then, pairwise comparisons were realized by using the proposed discriminability measures. Our results reveal the existence of time-frequency patterns which allows differentiating of sweep conditions from multifiber recordings.
  
  
  METHODS
  Currently, statistical methods used to justify significant differences in experimental conditions have rigorous criteria that must be met for correct validation of results. Discriminability measures proposed here are robust and can be adjusted to different experimental conditions (time series, repeated measures, specific variables and other).
  
  
  CONCLUSIONS
  Discriminability measures allowed determining the time intervals where two sweep situations have the highest probability to be differentiated from each other. High discriminability percentages were observed into protraction phase, although to a lesser degree, it was also observed in retraction phase. It was demonstrated that sensibility of discriminability measures are different. This revealing a greater ability to highlight percentage changes of pairwise comparisons. Finally, the methods here proposed can be adapted to other features of biological responses.
authors.affiliation: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET - UNT, and Departamento de Bioingeniería, Facultad de Ciencias Exactas y Tecnología, UNT Av. Independencia 1800, 4000 San Miguel de Tucumán, Argentina; Laboratorio de Medios e Interfases (LAMEIN), Universidad Nacional de Tucumán, Argentina. Electronic address: piza.ag@gmail.com.
authors.forename: Álvaro G
authors.initials: ÁG
authors.lastname: Pizá
authors.affiliation: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET - UNT, and Departamento de Bioingeniería, Facultad de Ciencias Exactas y Tecnología, UNT Av. Independencia 1800, 4000 San Miguel de Tucumán, Argentina; Laboratorio de Medios e Interfases (LAMEIN), Universidad Nacional de Tucumán, Argentina. Electronic address: ffarfan@herrera.unt.edu.ar.
authors.forename: Fernando D
authors.initials: FD
authors.lastname: Farfán
authors.affiliation: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET - UNT, and Departamento de Bioingeniería, Facultad de Ciencias Exactas y Tecnología, UNT Av. Independencia 1800, 4000 San Miguel de Tucumán, Argentina; Laboratorio de Medios e Interfases (LAMEIN), Universidad Nacional de Tucumán, Argentina. Electronic address: anaalbarracin@gmail.com.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Albarracín
authors.affiliation: Laboratorio de Medios e Interfases (LAMEIN), Universidad Nacional de Tucumán, Argentina. Electronic address: gruiz@herrera.unt.edu.ar.
authors.forename: Gabriel A
authors.initials: GA
authors.lastname: Ruiz
authors.affiliation: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET - UNT, and Departamento de Bioingeniería, Facultad de Ciencias Exactas y Tecnología, UNT Av. Independencia 1800, 4000 San Miguel de Tucumán, Argentina; Laboratorio de Medios e Interfases (LAMEIN), Universidad Nacional de Tucumán, Argentina. Electronic address: cfelice@herrera.unt.edu.ar.
authors.forename: Carmelo J
authors.initials: CJ
authors.lastname: Felice
mesh_terms: D000465:Algorithms; D000818:Animals; D004192:Discrimination, Psychological / Q000502:physiology*; D004558:Electric Stimulation / Q000379:methods; D004594:Electrophysiology / Q000379:methods*; D005154:Facial Nerve / Q000502:physiology; D005583:Fourier Analysis; D007257:Information Theory; D008839:Microelectrodes; D009043:Motor Activity / Q000502:physiology; D009475:Neurons, Afferent / Q000502:physiology*; D010812:Physical Stimulation / Q000379:methods*; D017208:Rats, Wistar; D012815:Signal Processing, Computer-Assisted; D013997:Time Factors; D014110:Touch / Q000502:physiology*; D014738:Vibrissae / Q000294:innervation / Q000502:physiology*
keywords: Afferent activity; Discriminability; Information theory; Spectrogram; Texture discrimination; Vibrissae