title: Direct identification ofMycobacterium abscessusthrough 16S rDNA sequence analysis and a citrate utilization test: A case report.
abstract: A growing number of nontuberculous mycobacteria infection cases, especially those caused by rapidly growing mycobacteria (RGM), have been reported in the past decade. Conventional methods for mycobacteria diagnosis are inefficient and easily lead to misdiagnosis. New detection methods, such as gene sequencing, have been reported but are not widely used. The aim of the present case report was to provide a quick and exact method of identifyingMyobacterium abscessus (M. abscessus)infections. The particular case reported in this study initially manifested as hyperglycemia and papules in the right leg. Routine cultures for fungus were repeatedly negative. However, cultures of the purulent material under aerobic cultivation for five days yielded a rapidly growing, nontuberculous mycobacterium. A Ziehl-Neelsen staining of this mycobacterium revealed the presence of acid-fast bacilli that were finally identified asM. abscessusthrough 16S rDNA sequence analysis and a citrate utilization test. The current report may help other clinicians to make a quick and accurate diagnosis of RGM infection.
authors.affiliation: Department of Microbiology and Immunology, Center of Laboratory Medicine, General Hospital of Chengdu Military Region of PLA, Chengdu 610083, P.R. China.
authors.forename: Ziying
authors.initials: Z
authors.lastname: Zou
authors.affiliation: Department of Microbiology and Immunology, Center of Laboratory Medicine, General Hospital of Chengdu Military Region of PLA, Chengdu 610083, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Microbiology and Immunology, Center of Laboratory Medicine, General Hospital of Chengdu Military Region of PLA, Chengdu 610083, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Department of Clinical Chemistry, Center of Laboratory Medicine, General Hospital of Chengdu Military Region of PLA, Chengdu 610083, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zeng
keywords: 16S rDNA sequence analysis; Mycobacterium abscessus; citrate utilization test; diabetes; rapidly growing mycobacteria