title: Enhanced phytate dephosphorylation by using Candida melibiosica yeast-based biofuel cell.
abstract: We report for the first time that Candida melibiosica expresses enhanced phytase activity when grown under biofuel cell polarization in a nutrient-poor medium, containing only fructose as a carbohydrate source. Phytase activity during the cultivation under polarization reached up to 25 U per g dry biomass, exceeding with 20 ± 3 % those of the control. A participation of the enzyme in the adaptation processes to the stress conditions is proposed. In addition, steady-state electrical outputs were achieved during biofuel cell operation at continuous polarization under constant load. The obtained results show that C. melibiosica yeast-based biofuel cell could be used for simultaneous electricity generation and phytate bioremediation.
authors.affiliation: Department of Biochemistry and Microbiology, "Paisii Hilendarski" University of Plovdiv, 24 Tzar Asen Str., 4000, Plovdiv, Bulgaria, jolinahubenova@yahoo.com.
authors.forename: Yolina
authors.initials: Y
authors.lastname: Hubenova
authors.forename: Danail
authors.initials: D
authors.lastname: Georgiev
authors.forename: Mario
authors.initials: M
authors.lastname: Mitov
mesh_terms: D010832:6-Phytase / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001674:Bioelectric Energy Sources / Q000382:microbiology; D002175:Candida / Q000201:enzymology*; D050260:Carbohydrate Metabolism; D003470:Culture Media / Q000737:chemistry; D004560:Electricity; D005656:Fungal Proteins / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D010833:Phytic Acid; D010832:6-Phytase