title: Effect of crude oil contamination on the chlorophyll content and morpho-anatomy of Cyperus brevifolius (Rottb.) Hassk.
abstract: Chlorophyll plays a pivotal role in the plant physiology and its productivity. Cultivation of plants in crude oil contaminated soil has a great impact on the synthesis of chlorophyll pigment. Morpho-anatomy of the experimental plant also shows structural deformation in higher concentrations. Keeping this in mind, a laboratory investigation has been carried out to study the effect of crude oil on chlorophyll content and morpho-anatomy of Cyperus brevifolius plant. Fifteen-day-old seedling of the plant was planted in different concentrations of the crude oil mixed soil (i.e., 10,000, 20,000, 30,000, 40,000, and 50,000 ppm). A control setup was also maintained without adding crude oil. Results were recorded after 6 months of plantation. Investigation revealed that there is a great impact of crude oil contamination on chlorophyll content of the leaves of the experimental plant. It also showed that chlorophyll a, chlorophyll b, and total chlorophyll content of leaves grown in different concentrations of crude oil were found to be lower than those of the control plant. Further, results also demonstrated that chlorophyll content was lowest in the treatment that received maximum dose of crude oil. It also showed that chlorophyll content was decreased with increased concentration of crude oil. Results also demonstrated that there was a reduction in plant shoot and root biomass with the increase of crude oil concentration. Results also revealed that the shoot biomass is higher than root biomass. Morphology and anatomy of the experimental plant also show structural deformation in higher concentrations. Accumulation of crude oil on the cuticle of the transverse section of the leaves and shoot forms a thick dark layer. Estimation of the level of pollution in an environment due to oil spill is possible by the in-depth study of the harmful effects of oil on the morphology and anatomy and chlorophyll content of the plants grown in that particular environment.
authors.affiliation: Environmental Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology (IASST), Paschim Boragaon, Guwahati, 781 035, Assam, India.
authors.forename: Plabita
authors.initials: P
authors.lastname: Baruah
authors.forename: Rashmi Rekha
authors.initials: RR
authors.lastname: Saikia
authors.forename: Partha Pratim
authors.initials: PP
authors.lastname: Baruah
authors.forename: Suresh
authors.initials: S
authors.lastname: Deka
mesh_terms: D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D029786:Cyperus / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D010578:Petroleum; D059392:Petroleum Pollution; D018515:Plant Leaves / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D018517:Plant Roots / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D018520:Plant Shoots / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D036226:Seedlings / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000494:pharmacology*; D013312:Stress, Physiological
chemical_list: D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants; D002734:Chlorophyll; C037184:chlorophyll b; D000077194:Chlorophyll A