title: Equine lamellar energy metabolism studied using tissue microdialysis.
abstract: Failure of lamellar energy metabolism may contribute to the pathophysiology of equine laminitis. Tissue microdialysis has the potential to dynamically monitor lamellar energy balance over time. The objectives of this study were to develop a minimally invasive lamellar microdialysis technique and use it to measure normal lamellar energy metabolite concentrations over 24 h. Microdialysis probes were placed (through the white line) into either the lamellar dermis (LAM) (n = 6) or the sublamellar dermis (SUBLAM) (n = 6) and perfused continuously over a 24 h study period. Probes were placed in the skin dermis (SKIN) for simultaneous comparison to LAM (n = 6). Samples were collected every 2 h and analysed for glucose, lactate, pyruvate, urea and glycerol concentrations. LAM was further compared with SUBLAM by simultaneous placement and sampling in four feet from two horses over 4 h. Horses were monitored for lameness, and either clinically evaluated for 1 month after probe removal (n = 4) or subjected to histological evaluation of the probe site (n = 10). There were no deleterious clinical effects of probe placement and the histological response was mild. Sample fluid recovery and metabolite concentrations were stable for 24 h. Glucose was lower (and lactate:glucose ratio higher) in LAM compared with SUBLAM and SKIN (P < 0.05). Pyruvate was lower in SUBLAM than SKIN and urea was lower in LAM than SKIN (P < 0.05). These differences suggest lower perfusion and increased glucose consumption in LAM compared with SUBLAM and SKIN. In conclusion, lamellar tissue microdialysis was well tolerated and may be useful for determining the contribution of energy failure in laminitis pathogenesis.
authors.affiliation: Australian Equine Laminitis Research Unit, School of Veterinary Science, Faculty of Science, University of Queensland, Gatton Campus, Gatton, QLD 4343, Australia.
authors.forename: C E
authors.initials: CE
authors.lastname: Medina-Torres
authors.affiliation: Australian Equine Laminitis Research Unit, School of Veterinary Science, Faculty of Science, University of Queensland, Gatton Campus, Gatton, QLD 4343, Australia.
authors.forename: C C
authors.initials: CC
authors.lastname: Pollitt
authors.affiliation: Australian Equine Laminitis Research Unit, School of Veterinary Science, Faculty of Science, University of Queensland, Gatton Campus, Gatton, QLD 4343, Australia.
authors.forename: C
authors.initials: C
authors.lastname: Underwood
authors.affiliation: Australian Equine Laminitis Research Unit, School of Veterinary Science, Faculty of Science, University of Queensland, Gatton Campus, Gatton, QLD 4343, Australia.
authors.forename: E M
authors.initials: EM
authors.lastname: Castro-Olivera
authors.affiliation: Australian Equine Laminitis Research Unit, School of Veterinary Science, Faculty of Science, University of Queensland, Gatton Campus, Gatton, QLD 4343, Australia.
authors.forename: S N
authors.initials: SN
authors.lastname: Collins
authors.affiliation: Australian Equine Laminitis Research Unit, School of Veterinary Science, Faculty of Science, University of Queensland, Gatton Campus, Gatton, QLD 4343, Australia.
authors.forename: R E
authors.initials: RE
authors.lastname: Allavena
authors.affiliation: New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348-1692, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Richardson
authors.affiliation: Australian Equine Laminitis Research Unit, School of Veterinary Science, Faculty of Science, University of Queensland, Gatton Campus, Gatton, QLD 4343, Australia. Electronic address: a.vaneps@uq.edu.au.
authors.forename: A W
authors.initials: AW
authors.lastname: van Eps
mesh_terms: D000818:Animals; D020405:Dermis / Q000378:metabolism*; D004734:Energy Metabolism; D005260:Female; D006724:Hoof and Claw / Q000378:metabolism*; D006736:Horses / Q000378:metabolism*; D008297:Male; D017551:Microdialysis / Q000662:veterinary*; D012016:Reference Values
keywords: Bioenergetics; Horse; Lamellae; Laminitis; Urea clearance