title: Astragalus membranaceus up-regulate Cosmc expression and reverse IgA dys-glycosylation in IgA nephropathy.
abstract: BACKGROUND
  Decreased Core I β3-Gal-T-specific molecular chaperone (Cosmc) expression induced IgA1 aberrant glycosylation is the main characteristic of IgA nephropathy (IgAN). This study tried to elucidate the effect of Astragalus membranaceus on Cosmc expression and IgA O-glycosylation of peripheral B lymphocytes in IgAN patients.
  
  
  METHODS
  Peripheral B lymphocytes of 21 IgAN patients and 10 normal controls were isolated and cultured with or without lipopolysaccharide (LPS) and Astragalus membranaceus injection (AMI). Cosmc mRNA and protein expression levels were measured by real-time RT-PCR and Western blot. IgA1 and glycosylation level were determined by enzyme-linked immunosorbent assay (ELISA) and VV lectin-binding method.
  
  
  RESULTS
  Cosmc mRNA expression and IgA1 O-glycosylation level in IgAN patients was significantly lower than normal controls at baseline. Treatment of LPS could obviously inhibit Cosmc expression and increase the IgA1 secretion in peripheral B lymphocytes of IgAN patients, which resulted in a significantly increase in IgA1 aberrant glycosylation level. Addition of AMI could remarkably up regulated Cosmc expression, decrease IgA1 secretion, and reverse glycosylation level in a dose related manner.
  
  
  CONCLUSIONS
  AMI can up-regulate Cosmc expression of peripheral B lymphocytes and reverse IgA1 aberrant O-glycosylation level, which might be the underlying mechanism of AMI therapy in treating IgAN.
  
  
  BACKGROUND
  TCTR20140515001 (Registration Date: 2014-05-15).
authors.forename: Ling
authors.initials: L
authors.lastname: Ji
authors.forename: XiaoLei
authors.initials: X
authors.lastname: Chen
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhong
authors.forename: Zi
authors.initials: Z
authors.lastname: Li
authors.forename: Lichuan
authors.initials: L
authors.lastname: Yang
authors.forename: Junming
authors.initials: J
authors.lastname: Fan
authors.forename: Wanxing
authors.initials: W
authors.lastname: Tang
authors.affiliation: Division of Nephrology, Department of Medicine, West China Hospital of Sichuan University, 37 # Guoxue road, Wuhou District, Chengdu, Sichuan, China. ddqstrike@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
mesh_terms: D000293:Adolescent; D000328:Adult; D027885:Astragalus propinquus / Q000737:chemistry*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D016022:Case-Control Studies; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005922:Glomerulonephritis, IGA / Q000097:blood* / Q000188:drug therapy*; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D018832:Molecular Chaperones / Q000096:biosynthesis* / Q000235:genetics; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects; D055815:Young Adult
chemical_list: C505369:C1GALT1C1 protein, human; D007070:Immunoglobulin A; D008070:Lipopolysaccharides; D018832:Molecular Chaperones; D010936:Plant Extracts; D012333:RNA, Messenger; C042441:glycosylated IgA