title: Genome-wide transcription analyses in Mycobacterium tuberculosis treated with lupulone.
abstract: Mycobacterium tuberculosis (M. tuberculosis), the causative agent of tuberculosis, still causes higher mortality than any other bacterial pathogen until now. With the emergence and spread of multidrug-resistant (MDR) and extensively drug-resistant (XDR-TB) strains, it becomes more important to search for alternative targets to develop new antimycobacterial drugs. Lupulone is a compound extracted from Hops (Hurnulus lupulus), which exhibits a good antimicrobial activity against M. tuberculosis with minimal inhibitory concentration (MIC) value of 10 μg/mL, but the response mechanisms of lupulone against M. tuberculosis are still poorly understood. In this study, we used a commercial oligonucleotide microarray to determine the overall transcriptional response of M. tuberculosis H37Rv triggered by exposure to MIC of lupulone. A total of 540 genes were found to be differentially regulated by lupulone. Of these, 254 genes were upregulated, and 286 genes were downregulated. A number of important genes were significantly regulated which are involved in various pathways, such as surface-exposed lipids, cytochrome P450 enzymes, PE/PPE multigene families, ABC transporters, and protein synthesis. Real-time quantitative RT-PCR was performed for choosed genes to verified the microarray results. To our knowledge, this genome-wide transcriptomics approach has produced the first insights into the response of M. tuberculosis to a lupulone challenge.
authors.affiliation: Life Science Department ChangChun Normal University Changchun P.R. China ; Bio-Reactor Center Jilin Agrichultural University Changchun P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Food Quality and Safety Key Laboratory of Zoonosis Research Institute of Zoonosis Ministry of Education Changchun P. R. China.
authors.forename: Junchao
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Food Quality and Safety Key Laboratory of Zoonosis Research Institute of Zoonosis Ministry of Education Changchun P. R. China.
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Food Quality and Safety Key Laboratory of Zoonosis Research Institute of Zoonosis Ministry of Education Changchun P. R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yuan
authors.affiliation: Jilin Enrty-Exit Inspection and Quarantine Bureau Changchun P.R. China.
authors.forename: Rizeng
authors.initials: R
authors.lastname: Meng
authors.affiliation: Key Lab for New Drug Research of TCM Research Institute of Tsinghua University in Shenzhen Shenzhen P.R. China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Food Quality and Safety Key Laboratory of Zoonosis Research Institute of Zoonosis Ministry of Education Changchun P. R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Food Quality and Safety Key Laboratory of Zoonosis Research Institute of Zoonosis Ministry of Education Changchun P. R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Guo
mesh_terms: D000995:Antitubercular Agents / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D046228:Microarray Analysis; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D013729:Terpenes / Q000378:metabolism*
chemical_list: D000995:Antitubercular Agents; D013729:Terpenes; C008299:lupulon
keywords: DNA microarray; antimycobacterial avticity; lupulone