title: Notch regulation of myogenic versus endothelial fates of cells that migrate from the somite to the limb.
abstract: Multipotent Pax3-positive (Pax3(+)) cells in the somites give rise to skeletal muscle and to cells of the vasculature. We had previously proposed that this cell-fate choice depends on the equilibrium between Pax3 and Foxc2 expression. In this study, we report that the Notch pathway promotes vascular versus skeletal muscle cell fates. Overactivating the Notch pathway specifically in Pax3(+) progenitors, via a conditional Pax3(NICD) allele, results in an increase of the number of smooth muscle and endothelial cells contributing to the aorta. At limb level, Pax3(+) cells in the somite give rise to skeletal muscles and to a subpopulation of endothelial cells in blood vessels of the limb. We now demonstrate that in addition to the inhibitory role of Notch signaling on skeletal muscle cell differentiation, the Notch pathway affects the Pax3:Foxc2 balance and promotes the endothelial versus myogenic cell fate, before migration to the limb, in multipotent Pax3(+) cells in the somite of the mouse embryo.
authors.affiliation: Department of Developmental and Stem Cell Biology, Centre National de la Recherche Scientifique, Unité de Recherche Associée 2578, and.
authors.forename: Alicia
authors.initials: A
authors.lastname: Mayeuf-Louchart
authors.affiliation: Department of Developmental and Stem Cell Biology, Centre National de la Recherche Scientifique, Unité de Recherche Associée 2578, and.
authors.forename: Mounia
authors.initials: M
authors.lastname: Lagha
authors.affiliation: Dynamic Imaging Platform, Imagopole, Institut Pasteur, 75015 Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Danckaert
authors.affiliation: Department of Developmental and Stem Cell Biology, Centre National de la Recherche Scientifique, Unité de Recherche Associée 2578, and.
authors.forename: Didier
authors.initials: D
authors.lastname: Rocancourt
authors.affiliation: Department of Developmental and Stem Cell Biology, Centre National de la Recherche Scientifique, Unité de Recherche Associée 2578, and.
authors.forename: Frederic
authors.initials: F
authors.lastname: Relaix
authors.affiliation: Department of Developmental and Stem Cell Biology, Centre National de la Recherche Scientifique, Unité de Recherche Associée 2578, and.
authors.forename: Stéphane D
authors.initials: SD
authors.lastname: Vincent
authors.affiliation: Department of Developmental and Stem Cell Biology, Centre National de la Recherche Scientifique, Unité de Recherche Associée 2578, and margaret.buckingham@pasteur.fr.
authors.forename: Margaret
authors.initials: M
authors.lastname: Buckingham
mesh_terms: D000483:Alleles; D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D042783:Endothelial Cells / Q000166:cytology*; D005121:Extremities / Q000196:embryology*; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005822:Genetic Vectors; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D024510:Muscle Development / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics*; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction; D019170:Somites / Q000196:embryology*
chemical_list: D051858:Forkhead Transcription Factors; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D051880:Receptors, Notch; C082078:mesenchyme fork head 1 protein; C068686:Pax3 protein, mouse