title: A fluorescent light-up probe with AIE characteristics for specific mitochondrial imaging to identify differentiating brown adipose cells.
abstract: We report the design and synthesis of a specific mitochondrial fluorescent probe AIE-MitoGreen-1 with AIE characteristics to monitor the mitochondrial morphology changes and identify the differentiation process of living brown adipose cells. The probe AIE-MitoGreen-1 has significant advantages such as high cellpermeability, good mitochondrial retention, low background fluorescence, large Stokes shift, and low toxicity.
authors.affiliation: Institute of Materials Research and Engineering (A*STAR), 3 Research Link, Singapore 117602.
authors.forename: Meng
authors.initials: M
authors.lastname: Gao
authors.forename: Choon Kiat
authors.initials: CK
authors.lastname: Sim
authors.forename: Chris Wai Tung
authors.initials: CW
authors.lastname: Leung
authors.forename: Qinglian
authors.initials: Q
authors.lastname: Hu
authors.forename: Guangxue
authors.initials: G
authors.lastname: Feng
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D002454:Cell Differentiation; D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D006834:Hydrazines / Q000737:chemistry* / Q000633:toxicity; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000473:pathology*; D011726:Pyridinium Compounds / Q000737:chemistry* / Q000633:toxicity
chemical_list: C000597459:AIE-MitoGreen-1; D005456:Fluorescent Dyes; D006834:Hydrazines; D011726:Pyridinium Compounds