title: Significance of the genetic polymorphism of CYP2D6 and NAT2 in patients with inflammatory bowel diseases.
abstract: BACKGROUND
  The main types of inflammatory bowel diseases (IBD) are ulcerative colitis (UC) and Crohn's disease (CD). There is evidence that, in addition to immunological and environmental factors, genetic factors also play an important role in the pathogenesis of IBD. Determination of polymorphism of CYP2D6 and NAT2 genes encoding I and II phase enzymes of xenobiotic biotransformation may have clinical value as an indicator of individual predisposition to diseases, and also contribute to effective and safe pharmacotherapy. The aim of this study was to investigate the association between genetic polymorphism of CYP2D6 and NAT2 and the incidence of IBD, including UC and CD, among inhabitants of central Poland.
  
  
  METHODS
  The study was performed in 258 individuals from central Poland (115 patients with IBD, including 65 patients with UC and 50 with CD; and in 143 healthy controls). The CYP2D6 genotypes of oxidation and NAT2 genotypes of acetylation were analyzed using the PCR-RFLP method.
  
  
  RESULTS
  There were no statistically significant differences in the frequency of the CYP2D6 genotypes and alleles in patients with IBD, UC and CD in comparison with the control group. The relative risk (OR) of IBD, UC and CD was higher in carriers of the allele NAT2*7 and was OR=3.49 (p=0.0019), OR=3.86 (p=0.0019), and OR=3.02 (p=0.0247), respectively.
  
  
  CONCLUSIONS
  Polymorphism of the gene encoding CYP2D6 does not affect the incidence of inflammatory bowel diseases. The carriers of the NAT2*7 allele which determines slow acetylation may be more predisposed to inflammatory bowel diseases, including ulcerative colitis and Crohn's disease.
authors.affiliation: Department of Pharmacogenetics, Medical University of Lodz, Łódź, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Dudarewicz
authors.affiliation: Department of Pharmacogenetics, Medical University of Lodz, Łódź, Poland.
authors.forename: Mariola
authors.initials: M
authors.lastname: Rychlik-Sych
authors.affiliation: Department of Pharmacogenetics, Medical University of Lodz, Łódź, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Barańska
authors.affiliation: Department of Pharmacogenetics, Medical University of Lodz, Łódź, Poland. Electronic address: anna.wojtczak@umed.lodz.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Wojtczak
authors.affiliation: Department of General and Colorectal Surgery, Medical University of Lodz, Łódź, Poland.
authors.forename: Radzisław
authors.initials: R
authors.lastname: Trzciński
authors.affiliation: Department of General and Colorectal Surgery, Medical University of Lodz, Łódź, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Dziki
authors.affiliation: Department of Pharmacogenetics, Medical University of Lodz, Łódź, Poland.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Skrętkowicz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*; D003424:Crohn Disease / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*; D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D011044:Poland / Q000453:epidemiology; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: D019389:Cytochrome P-450 CYP2D6; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human
keywords: CYP2D6; Genetic polymorphism; Inflammatory bowel diseases; NAT2