title: A possible iron delivery function of the dinuclear iron center of HcgD in [Fe]-hydrogenase cofactor biosynthesis.
abstract: HcgD, a homolog of the ubiquitous Nif3-like protein family, is found in a gene cluster involved in the biosynthesis of the iron-guanylylpyridinol (FeGP) cofactor of [Fe]-hydrogenase. The presented crystal structure and biochemical analyses indicated that HcgD has a dinuclear iron-center, which provides a pronounced binding site for anionic ligands. HcgD contains a stronger and a weaker bound iron; the latter being removable by chelating reagents preferentially in the oxidized state. Therefore, we propose HcgD as an iron chaperone in FeGP cofactor biosynthesis, which might also stimulate investigations on the functionally unknown but physiologically important eukaryotic Nif3-like protein family members.
authors.affiliation: Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße 10, 35043 Marburg, Germany.
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujishiro
authors.affiliation: Max Planck Institute for Biophysics, Max-von-Laue-Straße 3, 60438 Frankfurt/Main, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Ermler
authors.affiliation: Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße 10, 35043 Marburg, Germany; PRESTO, Japan Science and Technology Agency (JST), Honcho, Kawaguchi, Saitama 332-0012, Japan. Electronic address: shima@mpi-marburg.mpg.de.
authors.forename: Seigo
authors.initials: S
authors.lastname: Shima
mesh_terms: D019843:Archaeal Proteins / Q000378:metabolism*; D001692:Biological Transport; D002614:Chelating Agents / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D003067:Coenzymes / Q000096:biosynthesis*; D004492:Edetic Acid / Q000494:pharmacology; D006864:Hydrogenase / Q000737:chemistry* / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000378:metabolism*; D063747:Methanocaldococcus / Q000378:metabolism*; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: D019843:Archaeal Proteins; D002614:Chelating Agents; D003067:Coenzymes; D007506:Iron-Sulfur Proteins; D018832:Molecular Chaperones; D019343:Citric Acid; D004492:Edetic Acid; D007501:Iron; C118830:iron hydrogenase; D006864:Hydrogenase
keywords: Hydrogenase; Iron; Metal-binding protein; Metal-cofactor biosynthesis; Nif3-like protein family; X-ray crystallography