title: Off-axis digital holographic camera for quantitative phase microscopy.
abstract: We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a single shot. A thick holographic grating filters the beam containing the sample information in two dimensions through diffraction. The filtered beam creates the reference arm of the interferometer. The spatial filtering method, based on the high angular selectivity of the thick grating, reduces the alignment sensitivity to angular displacements compared with pinhole based Fourier filtering. The addition of a thin holographic grating alters the coherence plane tilt introduced by the thick grating so as to create high-visibility interference over the entire field of view. The acquired full-field off-axis holograms are processed to retrieve the amplitude and phase information of the sample. The system produces phase images of cheek cells qualitatively similar to phase images extracted with a standard commercial DHM.
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne, Laboratory of Applied Photonics Devices, CH-1015 Lausanne, Switzerland.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Monemhaghdoust
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne, Laboratory of Applied Photonics Devices, CH-1015 Lausanne, Switzerland ; Lyncée Tec SA, CH-1015 Lausanne, Switzerland.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Montfort
authors.affiliation: Lyncée Tec SA, CH-1015 Lausanne, Switzerland.
authors.forename: Yves
authors.initials: Y
authors.lastname: Emery
authors.affiliation: CDep engineering SARL, 1028 Préverenges, Switzerland.
authors.forename: Christian
authors.initials: C
authors.lastname: Depeursinge
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne, Laboratory of Applied Photonics Devices, CH-1015 Lausanne, Switzerland.
authors.forename: Christophe
authors.initials: C
authors.lastname: Moser
keywords: (050.1950) Diffraction gratings; (050.7330) Volume gratings; (090.1995) Digital holography; (110.0180) Microscopy; (180.3170) Interference microscopy; (330.6110) Spatial filtering