title: PARK7 protein translocating into spermatozoa mitochondria in Chinese asthenozoospermia.
abstract: PARK7 (DJ1) is a multifunctional oxidative stress response protein that protects cells against reactive oxygen species (ROS) and mitochondrial damage. PARK7 defects are known to cause various physiological dysfunctions, including infertility. Asthenozoospermia (AS), i.e. low-motile spermatozoa in the ejaculate, is a common cause of human male infertility. In this study, we found that downregulation of PARK7 resulted in increased levels of lipid peroxide and ROS, decreased mitochondrial membrane potential, and reduced mitochondrial complex I enzyme activity in the spermatozoa from AS patients. Furthermore, it was observed that PARK7 was translocated into the mitochondria of damaged spermatozoa in AS. Finally, we examined the oxidative state of PARK7 and the results demonstrated the enhancement of oxidation, expressed by increased sulfonic acid residues, the highest form of oxidation, as the sperm motility decreased. Taken together, these results revealed that PARK7 deficiency may increase the oxidative stress damage to spermatozoa. Our present findings open new avenues of therapeutic intervention targeting PARK7 for the treatment of AS.
authors.affiliation: State Key Laboratory of Natural and Biomimetic DrugsDepartment of Molecular and Cellular PharmacologySchool of Pharmaceutical Science, Peking University, Beijing, People's Republic of ChinaDepartment of AndrologyPeking University Third Hospital, Beijing 100191, People's Republic of ChinaState Key Laboratory of Natural and Biomimetic DrugsDepartment of Molecular and Cellular PharmacologySchool of Pharmaceutical Science, Peking University, Beijing, People's Republic of ChinaDepartment of AndrologyPeking University Third Hospital, Beijing 100191, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Natural and Biomimetic DrugsDepartment of Molecular and Cellular PharmacologySchool of Pharmaceutical Science, Peking University, Beijing, People's Republic of ChinaDepartment of AndrologyPeking University Third Hospital, Beijing 100191, People's Republic of ChinaState Key Laboratory of Natural and Biomimetic DrugsDepartment of Molecular and Cellular PharmacologySchool of Pharmaceutical Science, Peking University, Beijing, People's Republic of ChinaDepartment of AndrologyPeking University Third Hospital, Beijing 100191, People's Republic of China.
authors.forename: Wen-Jia
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Natural and Biomimetic DrugsDepartment of Molecular and Cellular PharmacologySchool of Pharmaceutical Science, Peking University, Beijing, People's Republic of ChinaDepartment of AndrologyPeking University Third Hospital, Beijing 100191, People's Republic of ChinaState Key Laboratory of Natural and Biomimetic DrugsDepartment of Molecular and Cellular PharmacologySchool of Pharmaceutical Science, Peking University, Beijing, People's Republic of ChinaDepartment of AndrologyPeking University Third Hospital, Beijing 100191, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Natural and Biomimetic DrugsDepartment of Molecular and Cellular PharmacologySchool of Pharmaceutical Science, Peking University, Beijing, People's Republic of ChinaDepartment of AndrologyPeking University Third Hospital, Beijing 100191, People's Republic of China.
authors.forename: Ren-Pei
authors.initials: RP
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Natural and Biomimetic DrugsDepartment of Molecular and Cellular PharmacologySchool of Pharmaceutical Science, Peking University, Beijing, People's Republic of ChinaDepartment of AndrologyPeking University Third Hospital, Beijing 100191, People's Republic of China pxp123@bjmu.edu.cn jianghui55@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Natural and Biomimetic DrugsDepartment of Molecular and Cellular PharmacologySchool of Pharmaceutical Science, Peking University, Beijing, People's Republic of ChinaDepartment of AndrologyPeking University Third Hospital, Beijing 100191, People's Republic of ChinaState Key Laboratory of Natural and Biomimetic DrugsDepartment of Molecular and Cellular PharmacologySchool of Pharmaceutical Science, Peking University, Beijing, People's Republic of ChinaDepartment of AndrologyPeking University Third Hospital, Beijing 100191, People's Republic of China pxp123@bjmu.edu.cn jianghui55@163.com.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Pu
mesh_terms: D000328:Adult; D044466:Asian People; D053627:Asthenozoospermia / Q000378:metabolism*; D002681:China; D015536:Down-Regulation; D042967:Electron Transport Complex I / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D000071617:Protein Deglycase DJ-1; D021381:Protein Transport / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism*; D055815:Young Adult
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D017382:Reactive Oxygen Species; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1; D042967:Electron Transport Complex I