title: A selectively fluorescein-based colorimetric probe for detecting copper(II) ion.
abstract: A novel fluorescein derivative 3-bromo-5-methylsalicylaldehyde fluorescein hydrazone (BMSFH) has been synthesized by reacting fluorescein hydrazide with 3-bromo-5-methylsalicylaldehyde and was developed as a new colorimetric probe for detection of Cu(2+). In the presence of Cu(2+) the BMSFH exhibits a rapid color change from colorless to yellow together with an obvious new band appeared at 502nm in the UV-vis absorption spectra. However, other common alkali-, alkaline earth-, transition- and rare earth metal ions induced no or minimal spectral changes. This change is attributed to BMSFH via coordination with Cu(2+) in a 1:1 stoichiometry and this binding to Cu(2+) is reversible, as indicated by the bleaching of the color when the Cu(2+) is extracted with EDTA. Experimental results indicate that the BMSFH can provide a rapid, selective and sensitive response to Cu(2+) with a linear dynamic range 3.0-330μmol/L and can be used as a potential Cu(2+) colorimetric probe in aqueous solution.
authors.affiliation: School of Arts and Sciences, Shanxi Agricultural University, Taigu 030801, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Agriculture, Shanxi Agricultural University, Taigu 030801, PR China. Electronic address: sxndzhangli@126.com.
authors.forename: Xianhong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D002413:Cations, Divalent / Q000032:analysis*; D003124:Colorimetry / Q000191:economics / Q000379:methods; D003300:Copper / Q000032:analysis*; D019793:Fluorescein / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D054879:Halogenation; D006835:Hydrazones / Q000737:chemistry*; D016014:Linear Models; D008745:Methylation
chemical_list: D000447:Aldehydes; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D006835:Hydrazones; C013243:salicylaldehyde; D003300:Copper; D019793:Fluorescein
keywords: 3-Bromo-5-methylsalicylaldehyde; Colorimetric; Copper; Fluorescein; Hydrazine; Probe