title: The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from Erlenmeyer flasks to a 2-L bioreactor.
abstract: The effect of two protein stabilizers (polyvinylpyrrolidone [PVP] and gelatine) on growth and 14D9 yield of Nicotiana tabacum cell suspension cultures (Ab-KDEL and sec-Ab) was analyzed. The addition of PVP at a concentration of 1.0 g L(-1) produced the highest total 14D9 yield (biomass + culture medium) in the Ab-KDEL line (4.82% total soluble protein [TSP]). With the addition of gelatine, the highest total 14D9 yield (2.48% TSP) was attained in the Ab-KDEL line at 5.0 g L(-1) gelatine. When the Ab-KDEL suspended cells were cultured in a 2-L bioreactor, the highest 14D9 yield was 8.1% TSP at a 5% w/v inoculum size, which was the best 14D9 yield so far obtained in the platforms tested (E. coli, N. tabacum leaves and seeds, N. tabacum hairy roots, and cell suspension cultures).
authors.affiliation: Cátedra de Farmacobotánica y Farmacognosia, Carreras de Farmacia y Bioquímica, Facultad de Ciencias de la Salud, Universidad Maimónides, Ciudad de Buenos Aires, Argentina.
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Marconi
authors.forename: María Alejandra
authors.initials: MA
authors.lastname: Alvarez
mesh_terms: D017151:Antibodies, Catalytic / Q000378:metabolism*; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D005780:Gelatin / Q000494:pharmacology; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D011205:Povidone / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
chemical_list: D017151:Antibodies, Catalytic; D011994:Recombinant Proteins; D005780:Gelatin; D011205:Povidone
keywords: Nicotiana tabacum; bioreactor; catalytic antibody 14D9; cell suspension cultures; hairy roots; in vitro plant cell cultures; protein additives