title: Electrospun DOXY-h loaded-poly(acrylic acid) nanofiber mats: in vitro drug release and antibacterial properties investigation.
abstract: Electrospun DOXY-h loaded-poly(acrylic acid) (PAA) nanofiber mats (PAA/DOXY-h nanofiber mats) were prepared by the electrospinning technique and post-spinning sorption method at various doses: PAA/DOXY-h125, PAA/DOXY-h250, PAA/DOXY-h500, and PAA/DOXY-h1000. The morphology, drug content, release characteristics, and antibacterial activities of the PAA/DOXY-h nanofiber mats were investigated with scanning electron microscopy, UV-vis spectrophotometry, and disc diffusion methodology. The PAA/DOXY-h nanofiber mats had a diameter range of 285-340 nm, and a smooth surface without beads. Adsorption isotherms of DOXY-h could be described well with the Freundlich model. The amounts of DOXY-h, after the post-spinning sorption process, in the PAA/DOXY-h nanofiber mats ranged between 27.57 and 101.71 mg/g. All of the PAA/DOXY-h nanofiber mats exhibited an initial burst release characteristic with cumulative releasing percentages between 37.14 and 45.97%, which followed the Fickian diffusion mechanism. Based on the antibacterial investigation, the tested gram-positive bacteria, Staphylococcus aureus and Streptococcus agalactiae, seemed to be more sensitive to PAA/DOXY-h nanofiber mats than the tested gram-negative bacteria, Pseudomonas aeruginosa. These PAA/DOXY-h nanofiber mats could be used as an antibacterial wound dressing.
authors.affiliation: a The Petroleum and Petrochemical College, Center of Excellence on Petrochemical and Materials Technology, Chulalongkorn University , Bangkok 10330 , Thailand.
authors.forename: Thitikan
authors.initials: T
authors.lastname: Khampieng
authors.forename: Gary E
authors.initials: GE
authors.lastname: Wnek
authors.forename: Pitt
authors.initials: P
authors.lastname: Supaphol
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D004318:Doxycycline / Q000737:chemistry* / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D007700:Kinetics; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D004337:Drug Carriers; C006903:carbopol 940; D004318:Doxycycline
keywords: doxycycline hyclate; electrospinning; in vitro drug release; poly(acrylic acid) nanofiber mats