title: Physiological and proteomic responses of different willow clones (Salix fragilis x alba) exposed to dredged sediment contaminated by heavy metals.
abstract: High biomass producing species are considered as tools for remediation of contaminated soils. Willows (Salix spp.) are prominent study subjects in this regard. In this study, different willow clones (Salix fragilis x alba) were planted on heavy-metal polluted dredging sludge. A first objective was assessment of the biomass production for these clones. Using a Gupta statistic, four clones were identified as high biomass producers (HBP). For comparison, a group of four clones with lowest biomass production were selected (LBP). A second objective was to compare metal uptake as well as the physiological and proteomic responses of these two groups. All these complementary data's allow us to have a better picture of the health of the clones that would be used in phytoremediation programs. Cd, Zn, and Ni total uptake was higher in the HBPs but Pb total uptake was higher in LBPs. Our proteomic and physiological results showed that the LBPs were able to maintain cellular activity as much as the HBPs although the oxidative stress response was more pronounced in the LBPs. This could be due to the high Pb content found in this group although a combined effect of the other metals cannot be excluded.
authors.forename: Aricia
authors.initials: A
authors.lastname: Evlard
authors.forename: Kjell
authors.initials: K
authors.lastname: Sergeant
authors.forename: Salvador
authors.initials: S
authors.lastname: Ferrandis
authors.forename: Bruno
authors.initials: B
authors.lastname: Printz
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
authors.forename: Cedric
authors.initials: C
authors.lastname: Guignard
authors.forename: Roger
authors.initials: R
authors.lastname: Paul
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Hausman
authors.forename: Bruno
authors.initials: B
authors.lastname: Campanella
mesh_terms: D001151:Arsenic / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018533:Biomass; D002241:Carbohydrates / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000737:chemistry; D019216:Metals, Heavy / Q000032:analysis / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D018515:Plant Leaves / Q000254:growth & development / Q000502:physiology; D018517:Plant Roots / Q000254:growth & development / Q000502:physiology; D040901:Proteomics; D032108:Salix / Q000254:growth & development / Q000502:physiology*; D012722:Sewage / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D013045:Species Specificity; D014197:Trees
chemical_list: D002241:Carbohydrates; D019216:Metals, Heavy; D012722:Sewage; D012989:Soil Pollutants; D001151:Arsenic; D010100:Oxygen