title: Development and validation a liquid chromatography mass spectrometry for determination of solasodine in rat plasma and its application to a pharmacokinetic study.
abstract: Solasodine is a poisonous alkaloid chemical compound that occurs in plants of the Solanaceae family. A simple and selective liquid chromatography mass spectrometry method for determination of solasodine in rat plasma was developed and validated over the range of 3-1,000 ng/mL. Chromatographic separation was achieved on a C18 (2.1 mm×50 mm, 3.5 μm) column with acetonitrile-0.1% formic acid in water as mobile phase with gradient elution. The flow rate was set at 0.4 mL/min. After addition of midazolam as internal standard (IS), liquid-liquid extraction by ethyl acetate was used as sample preparation. An electrospray ionization source was applied and operated in positive ion mode; selective ion monitoring mode was used for quantification with target ions m/z 414 for solasodine and m/z 326 for IS. Mean recoveries of solasodine in rat plasma were in the range of 87.6-94.1%. Matrix effects for solasodine were between 94.9% and 102.3%. Coefficient of variation of intra-day and inter-day precision were both <13%. The accuracy of the method ranged from 94.4% to 105.3%. The method was successfully applied to a pharmacokinetic study of solasodine after oral administration of 20mg/kg in rats.
authors.affiliation: Function Experiment Teaching Center of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Ma
authors.affiliation: Function Experiment Teaching Center of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Xitao
authors.initials: X
authors.lastname: Ding
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Sun
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Chongliang
authors.initials: C
authors.lastname: Lin
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: An
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Guanyang
authors.initials: G
authors.lastname: Lin
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, University-town, Wenzhou 325035, China. Electronic address: lankywang@163.com.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D008297:Male; D017207:Rats, Sprague-Dawley; D012991:Solanaceous Alkaloids / Q000008:administration & dosage / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D012991:Solanaceous Alkaloids; C012037:solasodine
keywords: LC–MS; Pharmacokinetics; Rat plasma; Solasodine