title: A new ELISA for the quantification of equine procalcitonin in plasma as potential inflammation biomarker in horses.
abstract: In human medicine, procalcitonin (PCT) is a very common and well-established biomarker for sepsis. Even though sepsis is also a leading cause of death in foals and adult horses, up to now, no data about the role of equine PCT in septic horses has been available. Based on monoclonal antibodies targeted against human PCT, we report here the development of a sandwich ELISA for the quantification of equine PCT in equine plasma samples. The ELISA was characterized for intra- and interassay variance and a working range from 25 to 1,000 ng mL(-1) was defined as within this range; both intra- and interassay variances were below 15 %. The target recovery ranged between 73 and 106 %. The ELISA was used to determine the equine PCT concentration in 24 healthy and 5 septic horses to show the potential for clinical evaluation of equine PCT. Significantly different (P = 0.0006) mean equine PCT concentrations were found for the healthy control group and the sepsis group (47 and 8,450 ng mL(-1)).
authors.affiliation: Research Unit Microbe-Plant Interactions, Department of Environmental Sciences, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstraße 1, 85764, Neuherberg, Germany, martin.rieger@helmholtz-muenchen.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Rieger
authors.forename: Christian
authors.initials: C
authors.lastname: Kochleus
authors.forename: Dana
authors.initials: D
authors.lastname: Teschner
authors.forename: Daniela
authors.initials: D
authors.lastname: Rascher
authors.forename: Ann Kristin
authors.initials: AK
authors.lastname: Barton
authors.forename: Arie
authors.initials: A
authors.lastname: Geerlof
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kremmer
authors.forename: Michael
authors.initials: M
authors.lastname: Schmid
authors.forename: Anton
authors.initials: A
authors.lastname: Hartmann
authors.forename: Heidrun
authors.initials: H
authors.lastname: Gehlen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015415:Biomarkers / Q000097:blood*; D002116:Calcitonin / Q000097:blood*; D015740:Calcitonin Gene-Related Peptide; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006736:Horses; D006801:Humans; D007249:Inflammation; D008297:Male; D011498:Protein Precursors / Q000097:blood*; D012372:ROC Curve; D011994:Recombinant Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D018805:Sepsis / Q000097:blood / Q000175:diagnosis*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; C000606589:CALCA protein, human; D011498:Protein Precursors; D011994:Recombinant Proteins; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide