title: First isolation of Neospora caninum from blood of a naturally infected adult dairy cow in Beijing, China.
abstract: Neospora caninum is an important cause of abortion in cattle worldwide, but the isolation of a viable parasite from an abortus is difficult, and viable N. caninum has not been isolated from any host in China. In the present study, peripheral blood samples were collected from a jugular vein of an adult dairy cow that had aborted; the cow was seropositive to N. caninum antibodies by ELISA. White blood cells were separated and seeded onto Vero cell monolayer cultures for parasite isolation. Tachyzoites were first observed in cell culture on day 84 after initial inoculation. The parasite was confirmed to be N. caninum by gene sequencing and immunofluorescence, and by bioassays in BALB/c mice. The new N. caninum isolate (NC-Bj) has a unique pattern on microsatellite Cont-14. To our knowledge, this is the first successful isolation of N. caninum in China from any host.
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Hao
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.forename: Xia
authors.initials: X
authors.lastname: Cui
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Daoyu
authors.initials: D
authors.lastname: Yang
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
mesh_terms: D037881:Aborted Fetus / Q000469:parasitology; D000034:Abortion, Veterinary / Q000097:blood / Q000469:parasitology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001681:Biological Assay / Q000662:veterinary; D001921:Brain / Q000196:embryology / Q000469:parasitology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000469:parasitology*; D002522:Chlorocebus aethiops; D003048:Coccidiosis / Q000097:blood / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000302:isolation & purification; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D018688:Neospora / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014709:Vero Cells
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D007074:Immunoglobulin G