title: Comparisons of five Saccharomyces cerevisiae strains for ethanol production from SPORL-pretreated lodgepole pine.
abstract: The performances of five yeast strains under three levels of toxicity were evaluated using hydrolysates from lodgepole pine pretreated by Sulfite Pretreatment to Overcome the Recalcitrance of Lignocelluloses (SPORL). The highest level of toxicity was represented by the whole pretreated biomass slurry, while intermediate toxicity was represented by the hydrolysate with partial loading of pretreatment spent liquor. The zero toxicity was represented using the enzymatic hydrolysate produced from thoroughly washed SPORL lodgepole pine solids. The results indicate that strains D5A and YRH400 can tolerate the whole pretreated biomass slurry to produce 90.1 and 73.5% theoretical ethanol yield. Strains Y1528, YRH403, and FPL450 did not grow in whole hydrolysate cultures and were observed to have lower ethanol productivities than D5A and YRH400 on the hydrolysate with intermediate toxicity. Both YRH400 and YRH403 were genetically engineered for xylose fermentation but were not able to consume xylose efficiently in hydrolysate.
authors.affiliation: School of Chemistry Chemical Eng., South China University Technol., Guangzhou, China; USDA Forest Service, Forest Products Laboratory, Madison, WI.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhou
authors.forename: Tianqing
authors.initials: T
authors.lastname: Lan
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Dien
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Hector
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhu
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology; D000431:Ethanol / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D009005:Monosaccharides / Q000378:metabolism; D028223:Pinus / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D013447:Sulfites / Q000737:chemistry* / Q000633:toxicity
chemical_list: D009005:Monosaccharides; D013447:Sulfites; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin
keywords: ethanol; inhibitors; pretreatment; toxicity; yeast fermentation