title: The pH optimum of native uracil-DNA glycosylase of Archaeoglobus fulgidus compared to recombinant enzyme indicates adaption to cytosolic pH.
abstract: Uracil-DNA glycosylase of Archaeoglobus fulgidus (Afung) in cell extracts exhibited maximal activity around pH 6.2 as compared to pH 4.8 for the purified recombinant enzyme expressed in Escherichia coli. Native Afung thus seems to be adapted to the intracellular pH of A. fulgidus, determined to be 7.0±0.1. Both recombinant and native Afung exhibited a broad temperature optimum for activity around 80°C, reflecting the A. fulgidus optimal growth temperature of 83°C. Adaption to the neutral conditions in the A. fulgidus cytoplasm might be due to covalent modifications or accessory factors, or due to a different folding when expressed in the native host.
authors.affiliation: Faculty of Science and Technology, Department of Mathematics and Natural Sciences-Centre for Organelle Research, University of Stavanger, Stavanger, Norway and Department of Biology and Centre for Geobiology, University of Bergen, Bergen, Norway.
authors.forename: Ingeborg
authors.initials: I
authors.lastname: Knævelsrud
authors.affiliation: Faculty of Science and Technology, Department of Mathematics and Natural Sciences-Centre for Organelle Research, University of Stavanger, Stavanger, Norway.
authors.forename: Sabina
authors.initials: S
authors.lastname: Kazazic
authors.affiliation: Department of Biology and Centre for Geobiology, University of Bergen, Bergen, Norway.
authors.forename: Nils-Kåre
authors.initials: NK
authors.lastname: Birkeland
authors.affiliation: Faculty of Science and Technology, Department of Mathematics and Natural Sciences-Centre for Organelle Research, University of Stavanger, Stavanger, Norway.
authors.forename: Svein
authors.initials: S
authors.lastname: Bjelland
mesh_terms: D000222:Adaptation, Physiological; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D019611:Archaeoglobus fulgidus / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D051981:Uracil-DNA Glycosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D011994:Recombinant Proteins; D051981:Uracil-DNA Glycosidase