title: Genomic imprinting analysis of Igf2/H19 in porcine cloned fetuses using parthenogenetic somatic cells as nuclear donors.
abstract: To gain insight into parthenogenesis in pigs, we report for the first time that using parthenogenetic somatic cells as nuclear donors (PSCNT), the porcine parthenogenetic fetus can develop to gestational day 39. Weight and morphological analysis revealed that PSCNT fetuses were smaller and developmentally retarded when compared to normally fertilized controls. Quantitative gene expression analysis indicated that in PSCNT fetuses, H19 was over-expressed, whereas Igf2 was significantly reduced (p < 0.05) compared with their controls. In addition, bisulfite-sequencing PCR results demonstrated that H19 differentially DNA methylated regions (DMRs) were hypomethylated in PSCNT fetuses, while Igf2 DMRs were hypermethylated in both PSCNT and control fetuses. Our results suggest that extended development of the porcine parthenogenetic fetus can be accomplished using PSCNT and that abnormal DNA methylation of H19 DMRs might contribute to the critical barrier of parthenogenesis in pigs.
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, 5333#, Xi'an Road, Changchun, 130062, China, wang_dong_xu@163.com.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Wang
authors.forename: Yuning
authors.initials: Y
authors.lastname: Song
authors.forename: Yongye
authors.initials: Y
authors.lastname: Huang
authors.forename: Feifei
authors.initials: F
authors.lastname: Duan
authors.forename: Qinyan
authors.initials: Q
authors.lastname: Lv
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
authors.forename: Liangxue
authors.initials: L
authors.lastname: Lai
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D019175:DNA Methylation; D005260:Female; D005333:Fetus / Q000002:abnormalities; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D010312:Parthenogenesis; D011247:Pregnancy; D062085:RNA, Long Noncoding / Q000235:genetics*; D013552:Swine / Q000196:embryology* / Q000235:genetics*
chemical_list: C094335:H19 long non-coding RNA; D062085:RNA, Long Noncoding; D007335:Insulin-Like Growth Factor II