title: Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies.
abstract: BACKGROUND
  Reprogrammed cells, including induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cells (NT-ESCs), are similar in many respects to natural embryonic stem cells (ESCs). However, previous studies have demonstrated that iPSCs retain a gene expression signature that is unique from that of ESCs, including differences in microRNA (miRNA) expression, while NT-ESCs are more faithfully reprogrammed cells and have better developmental potential compared with iPSCs.
  
  
  RESULTS
  We focused on miRNA expression and explored the difference between ESCs and reprogrammed cells, especially ESCs and NT-ESCs. We also compared the distinct expression patterns among iPSCs, NT-ESCs and NT-iPSCs. The results demonstrated that reprogrammed cells (iPSCs and NT-ESCs) have unique miRNA expression patterns compared with ESCs. The comparison of differently reprogrammed cells (NT-ESCs, NT-iPSCs and iPSCs) suggests that several miRNAs have key roles in the distinct developmental potential of reprogrammed cells.
  
  
  CONCLUSIONS
  Our data suggest that miRNAs play a part in the difference between ESCs and reprogrammed cells, as well as between MEFs and pluripotent cells. The variation of miRNA expression in reprogrammed cells derived using different reprogramming strategies suggests different characteristics induced by nuclear transfer and iPSC generation, as well as different developmental potential among NT-ESCs, iPSCs and NT-iPSCs.
authors.forename: Botao
authors.initials: B
authors.lastname: Zhao
authors.forename: Dehua
authors.initials: D
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
authors.forename: Jinsong
authors.initials: J
authors.lastname: Li
authors.forename: Chunsun
authors.initials: C
authors.lastname: Fan
authors.forename: Menggui
authors.initials: M
authors.lastname: Huang
authors.forename: Yi
authors.initials: Y
authors.lastname: Fan
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China. yan.jin@siat.ac.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Youxin
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D002460:Cell Line; D065150:Cellular Reprogramming; D002874:Chromosome Mapping; D053595:Embryonic Stem Cells / Q000502:physiology*; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D035683:MicroRNAs