title: A comparison of the thermal inactivation kinetics of human norovirus surrogates and hepatitis A virus in buffered cell culture medium.
abstract: Human noroviruses and hepatitis A virus (HAV) are considered as epidemiologically significant causes of foodborne disease. Therefore, studies are needed to bridge existing data gaps and determine appropriate parameters for thermal inactivation of human noroviruses and HAV. The objectives of this research were to compare the thermal inactivation kinetics of human norovirus surrogates (murine norovirus (MNV-1), and feline calicivirus (FCV-F9)) and HAV in buffered medium (2-ml vials), compare first-order and Weibull models to describe the data, calculate Arrhenius activation energy for each model, and evaluate model efficiency using selected statistical criteria. The D-values calculated from the first-order model (50-72 °C) ranged from 0.21-19.75 min for FCV-F9, 0.25-36.28 min for MNV-1, and 0.88-56.22 min for HAV. Using the Weibull model, the tD = 1 (time to destroy 1 log) for FCV-F9, MNV-1 and HAV at the same temperatures ranged from 0.10-13.27, 0.09-26.78, and 1.03-39.91 min, respectively. The z-values for FCV-F9, MNV-1, and HAV were 9.66 °C, 9.16 °C, and 14.50 °C, respectively, using the Weibull model. For the first order model, z-values were 9.36 °C, 9.32 °C, and 12.49 °C for FCV-F9, MNV-1, and HAV, respectively. For the Weibull model, estimated activation energies for FCV-F9, MNV-1, and HAV were 225, 278, and 182 kJ/mol, respectively, while the calculated activation energies for the first order model were 195, 202, and 171 kJ/mol, respectively. Knowledge of the thermal inactivation kinetics of norovirus surrogates and HAV will allow the development of processes that produce safer food products and improve consumer safety.
authors.affiliation: Department of Food Science and Technology, The University of Tennessee, 2600 River Drive, Knoxville, TN 37996-4591, USA.
authors.forename: Hayriye
authors.initials: H
authors.lastname: Bozkurt
authors.affiliation: Department of Food Science and Technology, The University of Tennessee, 2600 River Drive, Knoxville, TN 37996-4591, USA. Electronic address: ddsouza@utk.edu.
authors.forename: Doris H
authors.initials: DH
authors.lastname: D'Souza
authors.affiliation: Department of Food Science and Technology, The University of Tennessee, 2600 River Drive, Knoxville, TN 37996-4591, USA.
authors.forename: P Michael
authors.initials: PM
authors.lastname: Davidson
mesh_terms: D000818:Animals; D017927:Calicivirus, Feline / Q000737:chemistry / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry*; D030041:Hepatitis A virus / Q000737:chemistry / Q000254:growth & development*; D006801:Humans; D007700:Kinetics; D029322:Norovirus / Q000737:chemistry / Q000145:classification / Q000254:growth & development*; D013242:Sterilization / Q000295:instrumentation / Q000379:methods*; D013696:Temperature; D038301:Virus Inactivation
chemical_list: D003470:Culture Media
keywords: Activation energy; D-value and z-value; Human norovirus surrogates; Weibull model and first order model; hepatitis A virus