title: Effects of parathyroid hormone-related protein on osteogenic and adipogenic differentiation of human mesenchymal stem cells.
abstract: OBJECTIVE
  This work aims to investigate the effects of parathyroid hormone-related peptide (PTHrP) (1-86) on osteogenic and adipogenic differentiation of human mesenchymal stem cells (hMSCs) and the related mechanisms.
  
  
  METHODS
  hMSCs were isolated and cultured in vitro. They were divided into control group, osteogenesis group, adipogenesis group, osteogenesis+PTHrP group and adipogenesis+PTHrP group. The cell proliferation and differentiation, and expression levels of osteopontin (OPN) and lipoprotein lipase (LPL) mRNA were observed.
  
  
  RESULTS
  The proliferation rates of hMSCs in osteogenesis+PTHrP and adipogenesis+PTHrP group were significantly higher than that in control group, respectively (p < 0.01). The alkaline phosphatase (ALP)-positive osteoblasts firstly appeared in osteogenesis+PTHrP group, and Sudan IV-positive adipocytes firstly appeared in adipogenesis group. The expression level of OPN mRNA in osteogenesis+PTHrP group was significantly higher than that in osteogenesis group (p < 0.05), and that in adipogenesis+PTHrP group was also higher than adipogenesis group (p < 0.05). The expression level of LPL mRNA in osteogenesis+PTHrP group was significantly lower than that in osteogenesis group, and that in adipogenesis+PTHrP group was also lower than adipogenesis group (p < 0.05).
  
  
  CONCLUSIONS
  The osteogenesis and adipogenesis are related to each other during the induced differentiation of hMSCs. PTHrP (1-86) can promote the osteogenic differentiation and inhibits the adipogenic differentiation for hMSCs.
authors.affiliation: Department of Laboratory, Tianjin Hospital, Tianjin, China. guoshengxingcn@126.com.
authors.forename: K
authors.initials: K
authors.lastname: Zhang
authors.forename: F-J
authors.initials: FJ
authors.lastname: Zhang
authors.forename: W-J
authors.initials: WJ
authors.lastname: Zhao
authors.forename: G-S
authors.initials: GS
authors.lastname: Xing
authors.forename: X
authors.initials: X
authors.lastname: Bai
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis; D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008071:Lipoprotein Lipase / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis; D053495:Osteopontin / Q000096:biosynthesis / Q000235:genetics; D044162:Parathyroid Hormone-Related Protein / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; D012333:RNA, Messenger; C068871:parathyroid hormone-related protein (1-86); D053495:Osteopontin; D008071:Lipoprotein Lipase