title: Functional molecular lumino-materials to probe serum albumins: solid phase selective staining through noncovalent fluorescent labeling.
abstract: Selective staining of human serum albumin protein in gel electrophoresis over wide range of other protein(s) is extremely important because it contains more than 60% volume of serum fluid in human body. Given the nonexistence of suitable dye materials for selective staining of serum albumins in gel electrophoresis, we report a new class of easy synthesizable and low molecular weight staining agents based on 3-amino-N-alkyl-carbazole scaffold for selective staining of serum albumins in solid phase. A detailed structure-efficiency relationship (SER) study enabled us to develop two such potent functional molecular probes which stain both human and bovine serum albumin selectively in gel electrophoresis in the presence of other proteins and enzymes. The present gel staining process was found to be very simple and less time-consuming as compared to the conventional coomassie blue staining which in turn makes these probes a new class of serum albumin-specific staining materials in proteome research. Moreover, these molecular lumino-materials can detect serum albumins at subnanomolar level in the presence of broad spectrum of other proteins/enzymes in aqueous buffer (99.9% water, pH = 7.3) keeping the protein secondary structure intact. Our experimental and the docking simulation results show that these probes bind preferentially at 'binding site I' of both the serum proteins.
authors.affiliation: School of Basic Sciences, Indian Institute of Technology Mandi , Mandi 175001, HP, India.
authors.forename: Gourab
authors.initials: G
authors.lastname: Dey
authors.forename: Abhishek
authors.initials: A
authors.lastname: Gupta
authors.forename: Trinetra
authors.initials: T
authors.lastname: Mukherjee
authors.forename: Pankaj
authors.initials: P
authors.lastname: Gaur
authors.forename: Abhishek
authors.initials: A
authors.lastname: Chaudhary
authors.forename: Subhra Kanti
authors.initials: SK
authors.lastname: Mukhopadhyay
authors.forename: Chayan K
authors.initials: CK
authors.lastname: Nandi
authors.forename: Subrata
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D062105:Molecular Docking Simulation; D015335:Molecular Probes; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D012709:Serum Albumin