title: Pctaire1/Cdk16 promotes skeletal myogenesis by inducing myoblast migration and fusion.
abstract: The Cdk-related protein kinase Pctaire1/Cdk16 is abundantly expressed in brain, testis and skeletal muscle. Functional roles of Pctaire1 such as regulation of neuron migration and neurite outgrowth thus far have been mainly elucidated in the field of nervous system development. Although these regulations based on cytoskeletal rearrangements evoke a possible role of Pctaire1 in the development of skeletal muscle, little is known in this regard. In this study, we demonstrated that myogenic differentiation and subsequent fusion is promoted in Pctaire1 overexpressing cells, and conversely, is inhibited in the knockdown cells. Furthermore, our findings suggest that Pctaire1 exerts promyogenic effects by regulating myoblast migration and process formation during skeletal myogenesis.
authors.affiliation: Division of Cell-Free Sciences, Proteo-Science Center, Ehime University, Matsuyama 790-8577, Japan.
authors.forename: Kouhei
authors.initials: K
authors.lastname: Shimizu
authors.affiliation: Division of Cell-Free Sciences, Proteo-Science Center, Ehime University, Matsuyama 790-8577, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Uematsu
authors.affiliation: Division of Integrative Pathophysiology, Proteo-Science Center, Graduate School of Medicine, Ehime University, Toon 791-0295, Japan.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Imai
authors.affiliation: Division of Cell-Free Sciences, Proteo-Science Center, Ehime University, Matsuyama 790-8577, Japan; The Venture Business Laboratory, Ehime University, Matsuyama 790-8577, Japan. Electronic address: sawasaki@ehime-u.ac.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sawasaki
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002459:Cell Fusion; D002460:Cell Line; D002465:Cell Movement; D018844:Cyclin-Dependent Kinases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000166:cytology* / Q000254:growth & development*; D032446:Myoblasts / Q000166:cytology*; D015854:Up-Regulation
chemical_list: D018844:Cyclin-Dependent Kinases; C089463:PCTAIRE-1 protein kinase
keywords: Migration; Pctaire1/Cdk16; Process formation; Skeletal muscle differentiation