title: Small RNA library cloning procedure for deep sequencing of specific endogenous siRNA classes in Caenorhabditis elegans.
abstract: In recent years, distinct classes of small RNAs ranging in size from ~21 to 26 nucleotides have been discovered and shown to play important roles in a wide array of cellular functions. Because of the abundance of these small RNAs, library preparation from an RNA sample followed by deep sequencing provides the identity and quantity of a particular class of small RNAs. In this chapter we describe a detailed protocol for preparing small RNA libraries for deep sequencing on the Illumina platform from the nematode C. elegans.
authors.affiliation: Department of Biology, Syracuse University, 110 Life Sciences Complex, 107 College Place, Syracuse, NY, 13244, USA.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Ow
authors.forename: Nelson C
authors.initials: NC
authors.lastname: Lau
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Hall
mesh_terms: D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics*; D048348:Reverse Transcription
chemical_list: D034741:RNA, Small Interfering