title: A Hg(II)-mediated "signal-on" electrochemical glutathione sensor.
abstract: We report the design and fabrication of a DNA-based electrochemical sensor for detection of glutathione. Sensor signaling relies on glutathione's ability to chelate mercury Hg(II), displacing it from the thymine-Hg(II)-thymine complex formed between the surface-immobilized DNA probes. Our results show that this sensor is sensitive and selective enough to be employed in saliva.
authors.affiliation: Department of Chemistry, Hamilton Hall, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA. rlai2@unl.edu.
authors.forename: Hamid R
authors.initials: HR
authors.lastname: Lotfi Zadeh Zhad
authors.forename: Rebecca Y
authors.initials: RY
authors.lastname: Lai
mesh_terms: D015374:Biosensing Techniques; D015342:DNA Probes / Q000737:chemistry; D055664:Electrochemical Techniques; D005978:Glutathione / Q000032:analysis*; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D008628:Mercury / Q000737:chemistry*; D013941:Thymine / Q000737:chemistry
chemical_list: D015342:DNA Probes; D061925:Immobilized Nucleic Acids; D008628:Mercury; D005978:Glutathione; D013941:Thymine