title: Pretreating lignocellulosic biomass by the concentrated phosphoric acid plus hydrogen peroxide (PHP) for enzymatic hydrolysis: evaluating the pretreatment flexibility on feedstocks and particle sizes.
abstract: In order to seek a high-efficient pretreatment path for converting lignocellulosic feedstocks to fermentable sugars by enzymatic hydrolysis, the concentrated H₃PO₄ plus H₂O₂ (PHP) was attempted to pretreat different lignocellulosic biomass for evaluating the pretreatment flexibility on feedstocks. Meanwhile, the responses of pretreatment to particle sizes were also evaluated. When the PHP-pretreatment was employed (final H₂O₂ and H₃PO₄ concentration of 1.77% and 80.0%), 71-96% lignin and more than 95% hemicellulose in various feedstocks (agricultural residues, hardwood, softwood, bamboo, and their mixture, and garden wastes mixture) can be removed. Consequently, more than 90% glucose conversion was uniformly achieved indicating PHP greatly improved the pretreatment flexibility to different feedstocks. Moreover, when wheat straw and oak chips were PHP-pretreated with different sizes, the average glucose conversion reached 94.9% and 100% with lower coefficient of variation (7.9% and 0.0%), which implied PHP-pretreatment can significantly weaken the negative effects of feedstock sizes on subsequent conversion.
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China; Engineering & Technology Center for Rural Environment Protection of Sichuan Province, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China; Engineering & Technology Center for Rural Environment Protection of Sichuan Province, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China.
authors.forename: Zhanghong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China; Engineering & Technology Center for Rural Environment Protection of Sichuan Province, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China. Electronic address: fishensjtu@gmail.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Shen
authors.affiliation: Forest Products Biotechnology, Department of Wood Science, The University of British Columbia, 2424 Main Mall, Vancouver, BC, Canada.
authors.forename: Jinguang
authors.initials: J
authors.lastname: Hu
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
authors.forename: Fubao
authors.initials: F
authors.lastname: Sun
authors.affiliation: Engineering & Technology Center for Rural Environment Protection of Sichuan Province, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Lin
authors.affiliation: Engineering & Technology Center for Rural Environment Protection of Sichuan Province, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Engineering & Technology Center for Rural Environment Protection of Sichuan Province, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China.
authors.forename: Yanzong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China; Engineering & Technology Center for Rural Environment Protection of Sichuan Province, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China.
authors.forename: Shihuai
authors.initials: S
authors.lastname: Deng
mesh_terms: D056804:Biofuels; D018533:Biomass; D002851:Chromatography, High Pressure Liquid; D003246:Conservation of Energy Resources / Q000379:methods; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D010316:Particle Size; D010756:Phosphoric Acids / Q000737:chemistry*; D014994:Xylose / Q000032:analysis / Q000737:chemistry
chemical_list: D056804:Biofuels; D010756:Phosphoric Acids; C036909:lignocellulose; D008031:Lignin; D014994:Xylose; D006861:Hydrogen Peroxide; C030242:phosphoric acid; D005947:Glucose
keywords: Concentrated phosphoric acid; Enzymatic hydrolysis; Hydrogen peroxide; Pretreatment