title: A method for assessing mitochondrial bioenergetics in whole white adipose tissues.
abstract: Obesity is a primary risk factor for numerous metabolic diseases including metabolic syndrome, type II diabetes (T2DM), cardiovascular disease and cancer. Although classically viewed as a storage organ, the field of white adipose tissue biology is expanding to include the consideration of the tissue as an endocrine organ and major contributor to overall metabolism. Given its role in energy production, the mitochondrion has long been a focus of study in metabolic dysfunction and a link between the organelle and white adipose tissue function is likely. Herein, we present a novel method for assessing mitochondrial bioenergetics from whole white adipose tissue. This method requires minimal manipulation of tissue, and eliminates the need for cell isolation and culture. Additionally, this method overcomes some of the limitations to working with transformed and/or isolated primary cells and allows for results to be obtained more expediently. In addition to the novel method, we present a comprehensive statistical analysis of bioenergetic data as well as guidelines for outlier analysis.
authors.affiliation: Department of Pathology, Division of Molecular and Cellular Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, United States of America.
authors.forename: Kimberly J
authors.initials: KJ
authors.lastname: Dunham-Snary
authors.affiliation: Department of Biostatistics, Section on Statistical Genetics, The University of Alabama at Birmingham, Birmingham, AL 35294, United States of America.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Sandel
authors.affiliation: Department of Pathology, Division of Molecular and Cellular Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, United States of America.
authors.forename: David G
authors.initials: DG
authors.lastname: Westbrook
authors.affiliation: Department of Pathology, Division of Molecular and Cellular Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, United States of America.
authors.forename: Scott W
authors.initials: SW
authors.lastname: Ballinger
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002318:Cardiovascular Diseases / Q000378:metabolism / Q000473:pathology; D004734:Energy Metabolism / Q000187:drug effects; D008297:Male; D008659:Metabolic Diseases / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism*; D012402:Rotenone / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D012402:Rotenone; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
keywords: Bioenergetics; Mitochondrion; White adipose tissue