title: Unique Responses are Observed in Transient Receptor Potential Ankyrin 1 and Vanilloid 1 (TRPA1 and TRPV1) Co-Expressing Cells.
abstract: Transient receptor potential (TRP) ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are implicated in modulation of cough and nociception. In vivo, TRPA1 and TRPV1 are often co-expressed in neurons and TRPA1V1 hetero-tetramer formation is noted in cells co-transfected with the respective expression plasmids. In order to understand the impact of TRP receptor interaction on activity, we created stable cell lines expressing the TRPA1, TRPV1 and co-expressing the TRPA1 and TRPV1 (TRPA1V1) receptors. Among the 600 compounds screened against these receptors, we observed a number of compounds that activated the TRPA1, TRPV1 and TRPA1V1 receptors; compounds that activated TRPA1 and TRPA1V1; compounds that activated TRPV1 and TRPA1V1; compounds in which TRPA1V1 response was modulated by either TRPA1 or TRPV1; and compounds that activated only TRPV1 or TRPA1 or TRPA1V1; and one compound that activated TRPA1 and TRPV1, but not TRPA1V1. These results suggest that co-expression of TRPA1 and TRPV1 receptors imparts unique activation profiles different from that of cells expressing only TRPA1 or TRPV1.
authors.affiliation: Cardiovascular and Respiratory Studies, The University of Hull, HU6 7RX, UK. Sadofsky@hull.ac.uk.
authors.forename: Laura R
authors.initials: LR
authors.lastname: Sadofsky
authors.affiliation: The Procter & Gamble Company, Mason, OH 45040, USA. Sreekrishna.k@pg.com.
authors.forename: Koti T
authors.initials: KT
authors.lastname: Sreekrishna
authors.affiliation: The Procter & Gamble Company, Mason, OH 45040, USA. lin.y.2@Pg.com.
authors.forename: Yakang
authors.initials: Y
authors.lastname: Lin
authors.affiliation: The Procter & Gamble Company, Mason, OH 45040, USA. schinaman.cr@pg.com.
authors.forename: Renee
authors.initials: R
authors.lastname: Schinaman
authors.affiliation: The Procter & Gamble Company, Mason, OH 45040, USA. gorka.k@pg.com.
authors.forename: Kate
authors.initials: K
authors.lastname: Gorka
authors.affiliation: The Procter & Gamble Company, Mason, OH 45040, USA. mantri.y@pg.com.
authors.forename: Yogita
authors.initials: Y
authors.lastname: Mantri
authors.affiliation: The Procter & Gamble Company, Mason, OH 45040, USA. haught.c@pg.com.
authors.forename: John Christian
authors.initials: JC
authors.lastname: Haught
authors.affiliation: The Procter & Gamble Company, Mason, OH 45040, USA. huggins.tg@pg.com.
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Huggins
authors.affiliation: The Procter & Gamble Company, Mason, OH 45040, USA. Isfort.rj@pg.com.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Isfort
authors.affiliation: The Procter & Gamble Company, Mason, OH 45040, USA. bascom.cc@pg.com.
authors.forename: Charles C
authors.initials: CC
authors.lastname: Bascom
authors.affiliation: Cardiovascular and Respiratory Studies, The University of Hull, HU6 7RX, UK. A.H.Morice@hull.uk.
authors.forename: Alyn H
authors.initials: AH
authors.lastname: Morice