title: In vitro investigation of antioxidant, anti-Inflammatory, and antiplatelet adhesion properties of genistein-modified poly(ethersulfone)/poly(vinylpyrrolidone) hemodialysis membranes.
abstract: Hemocompatibility of genistein-modified poly(ethersulfone)/poly(vinylpyrrolidone) (PES/PVP) hemodialysis (HD) membranes has been investigated in vitro with emphasis on evaluation of cell viability, antioxidant, anti-inflammatory, and antiplatelet adhesion properties. Genistein modified PES/PVP membranes reveal significant reduction of the reactive oxygen species and also considerable suppression of interleukin-1β and tumor necrosis factor-α levels in whole blood, but to a lesser extent ininterleukin-6. The incorporation of PVP into the HD membrane reduces platelet adhesion by virtue of its hydrophilicity. Of particular importance is that platelet adhesion of the genistein modified membranes declines noticeably at low concentrations of genistein for about 5-10%, beyond which it raises the number of adhered platelets. The initial decline in the platelet adhesion is attributable to genistein's ability to inhibit intercellular and/or vascular cell adhesion, whereas the reversal of this adhesion trend with further increase of genistein loading is ascribed to the inherent hydrophobicity of the genistein modified HD membrane.
authors.affiliation: Department of Polymer Engineering, University of Akron, Akron, Ohio, 44325.
authors.forename: Teng
authors.initials: T
authors.lastname: Chang
authors.forename: Linda
authors.initials: L
authors.lastname: DeFine
authors.forename: Thomas
authors.initials: T
authors.lastname: Alexander
authors.forename: Thein
authors.initials: T
authors.lastname: Kyu
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology* / Q000633:toxicity; D001672:Biocompatible Materials / Q000494:pharmacology*; D001773:Blood Cells / Q000187:drug effects / Q000378:metabolism; D019833:Genistein / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D066298:In Vitro Techniques; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008422:Materials Testing; D008567:Membranes, Artificial; D015394:Molecular Structure; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D011205:Povidone / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism; D006435:Renal Dialysis / Q000295:instrumentation*; D013450:Sulfones / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001672:Biocompatible Materials; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D008567:Membranes, Artificial; D011108:Polymers; D017382:Reactive Oxygen Species; D013450:Sulfones; D014409:Tumor Necrosis Factor-alpha; C022840:polyether sulfone; D019833:Genistein; D011205:Povidone
keywords: anti-inflammatory; antioxidant; antiplatelet adhesion; genistein; hemodialysis