title: 25-Hydroxycholecalciferol supplementation improves growth performance and decreases inflammation during an experimental lipopolysaccharide injection.
abstract: Three experiments were conducted to study the effects of 25-hydroxycholecalciferol supplementation on BW gain, IL-1β, and 1α-hydroxylase mRNA expression in different organs of broiler chickens following a lipopolysaccharide (LPS) injection. In experiment I, birds were fed a basal diet supplemented with either cholecalciferol (3,000 IU/kg) or 25-hydroxycholecalciferol (69 µg/kg). At 21 and 35 d of age, birds were injected with LPS. Post-LPS injection, birds supplemented with 25-hydroxycholecalciferol gained approximately 2.5% (P = 0.03) and 3.8% (P < 0.01), respectively, more BW than the birds supplemented with cholecalciferol over the 24-h period. In experiment II, birds were fed basal diets supplemented with 25-hydroxycholecalciferol at 6.25, 25, and 50 µg/kg of feed or cholecalciferol at 250 IU/kg of feed. At 35 d of age, birds were injected with LPS. Birds fed 25-hydroxycholecalciferol at 25 and 50 µg/kg and injected with LPS had approximately 7-fold and 3-fold less (P = 0.010) IL-1β mRNA in the liver compared with those birds fed 6.25 µg/kg of 25-hydroxycholecalciferol and the cholecalciferol (250 IU/kg) group. In experiment III, birds were fed a basal diet supplemented with either cholecalciferol (3,000 IU/kg) or 25-hydroxycholecalciferol (69 µg/kg). At 28 d of age, birds were fed 25-hydroxycholecalciferol and injected with LPS had 1.1-fold less (P < 0.01) IL-1β mRNA in the liver than the cholecalciferol-fed group. After an LPS injection, birds supplemented with 25-hydroxycholecalciferol had increased 1α-hydroxylase mRNA amounts in the liver (P = 0.07). In conclusion, 25-hydroxycholecalciferol supplementation at higher doses improved growth performance and decreased inflammatory gene IL-1β mRNA amounts in the liver post-LPS injection.
authors.affiliation: Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster 44691.
authors.forename: Antrison
authors.initials: A
authors.lastname: Morris
authors.affiliation: Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster 44691.
authors.forename: Revathi
authors.initials: R
authors.lastname: Shanmugasundaram
authors.affiliation: Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster 44691.
authors.forename: Mike S
authors.initials: MS
authors.lastname: Lilburn
authors.affiliation: Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster 44691 selvaraj.7@osu.edu.
authors.forename: Ramesh K
authors.initials: RK
authors.lastname: Selvaraj
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002112:Calcifediol; D002645:Chickens / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000139:chemically induced / Q000662:veterinary*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides; D008099:Liver / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects*
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D002112:Calcifediol
keywords: 25-hydroxycholecalciferol; inflammation; lipopolysaccharide