title: The ARID-HMG DNA-binding protein AtHMGB15 is required for pollen tube growth in Arabidopsis thaliana.
abstract: In flowering plants, male gametes (sperm cells) develop within male gametophytes (pollen grains) and are delivered to female gametes for double fertilization by pollen tubes. Therefore, pollen tube growth is crucial for reproduction. The mechanisms that control pollen tube growth remain poorly understood. In this study, we demonstrated that the ARID-HMG DNA-binding protein AtHMGB15 plays an important role in pollen tube growth. This protein is preferentially expressed in pollen grains and pollen tubes and is localized in the vegetative nuclei of the tricellular pollen grains and pollen tubes. Knocking down AtHMGB15 expression via a Ds insertion caused retarded pollen tube growth, leading to a significant reduction in the seed set. The athmgb15-1 mutation affected the expression of 1686 genes in mature pollen, including those involved in cell wall formation and modification, cell signaling and cellular transport during pollen tube growth. In addition, it was observed that AtHMGB15 binds to DNA in vitro and interacts with the transcription factors AGL66 and AGL104, which are required for pollen maturation and pollen tube growth. These results suggest that AtHMGB15 functions in pollen tube growth through the regulation of gene expression.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China; Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture, The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Xia
authors.forename: Yu-Jiao
authors.initials: YJ
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Qian-Kun
authors.initials: QK
authors.lastname: Niu
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Tan
authors.forename: Liang-Cui
authors.initials: LC
authors.lastname: Chu
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Chen
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Zhang
authors.forename: De
authors.initials: D
authors.lastname: Ye
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D019281:Dimerization; D005306:Fertilization; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D010641:Phenotype; D011058:Pollen / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D053205:Pollen Tube / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D054817:Pollination; D025941:Protein Interaction Mapping; D012098:Reproduction; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C000597884:HMGB15 protein, Arabidopsis; D014157:Transcription Factors
keywords: ARID; Arabidopsis; HMGB; gametophyte; pollen tube; transcription factor