title: Identification of bacteria pathogenic to or associated with onion (Allium cepa) based on sequence differences in a portion of the conserved gyrase B gene.
abstract: We have developed a method for the identification of Gram-negative bacteria, particularly members of the Enterobacteriaceae, based on sequence variation in a portion of the gyrB gene. Thus, we identified, in most cases to species level, over 1000 isolates from onion bulbs and leaves and soil in which onions were grown.
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Jean M
authors.initials: JM
authors.lastname: Bonasera
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Jo Ann E
authors.initials: JE
authors.lastname: Asselin
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA. Electronic address: svb1@cornell.edu.
authors.forename: Steven V
authors.initials: SV
authors.lastname: Beer
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D044822:Biodiversity; D017124:Conserved Sequence; D027081:DNA Gyrase / Q000737:chemistry / Q000235:genetics*; D057166:High-Throughput Screening Assays; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods; D019697:Onions / Q000382:microbiology*; D054730:Protein Interaction Domains and Motifs; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D027081:DNA Gyrase
keywords: Gyrase B; Identification-bacteria; Onion; PCR; Pathogenic-bacteria