title: Cyclin D1 expression is correlated with cell differentiation and cell proliferation in oral squamous cell carcinomas.
abstract: The present study conducted an immunohistochemical investigation of cyclin D1 and Ki-67 expression in oral squamous cell carcinoma (SCC) to evaluate the correlations between cell differentiation, cell proliferation and metastasis, and the effect of anticancer drug medication and cyclin D1 expression. Cyclin D1 and Ki-67 were detected clearly in the nuclei of 35 SCC samples. No correlation between cyclin D1 protein expression and oral SCC differentiation was found. By contrast, the majority of metastatic foci (90%) exhibited strong cyclin D1 expression, whereas weak expression was observed in metastatic foci with pre-operative adjuvant therapy. Additionally, cyclin D1 and Ki-67 were expressed in basal to suprabasal cells of well-differentiated oral SCC, whereas cyclin D1-positive and Ki-67-negative cells were present in the highly-differentiated region, according to a double-immunostaining method. These results indicate that the expression of cyclin D1 protein plays a role in cell differentiation and cell proliferation in well-differentiated oral SCC.
authors.affiliation: Second Department of Oral and Maxillofacial Surgery, Osaka Dental University, Hirakata, Osaka 573-1121, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ohnishi
authors.affiliation: Second Department of Oral and Maxillofacial Surgery, Osaka Dental University, Hirakata, Osaka 573-1121, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Department of Oral Pathology, Osaka Dental University, Hirakata, Osaka 573-1121, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Wato
authors.affiliation: Department of Oral Pathology, Osaka Dental University, Hirakata, Osaka 573-1121, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: Second Department of Oral and Maxillofacial Surgery, Osaka Dental University, Hirakata, Osaka 573-1121, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kakudo
authors.affiliation: Department of Cell Biology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Nozaki
keywords: Ki-67; cyclin D1; differentiation; metastasis; oral squamous cell carcinoma; pre-operative adjuvant therapy