title: [Immunosuppressive action of dendritic cells sensitized by oligodeoxynucleotides and CA125 on human OVCAR3 ovarian carcinoma xenografts in nude mice].
abstract: OBJECTIVE
  To explore the immunosuppressive action of dendritic cells (DC) sensitized by oligodeoxynucleotides containing "un-methylated cytosine phosphodiester bond-guanylic acid" motif (CpG ODN) and CA125 on human ovarian carcinoma xenografts in nude mice.
  
  
  METHODS
  Human peripheral blood-derived dendritic cells were isolated and identified by flow cytometry. The DC sensitized by CpG ODN and CA125 were then co-cultured with T cells and finally cytotoxic T lymphocytes (CTL) were induced. Nude mice bearing OVCAR3 transplanted tumor were immunized with induced CTL by subcutaneous injection and tumor growth and cell apoptosis observed.
  
  
  RESULTS
  Premature DC had a low expression of CD83 and CD86. In vivo, delayed growth of OVCAR3 xenografts was observed after immunotherapy with CTL induced by DC pulsed by CpG ODN and CA125. The inhibition rate of tumor was 50.71% and it was better than CpG ODN-pulsed and CA125-pulsed groups (P < 0.05). Cell apoptotic rate was (29.6 ± 3.0)% in CpG ODN+CA125-pulsed group versus (21.8 ± 2.7)% in CpG ODN-pulsed group. And both were more than those of unpulsed and CA125-pulsed groups (P < 0.05).
  
  
  CONCLUSIONS
  CTL induced by DC sensitized by CpG ODN and CA125 can inhibit the growth of ovarian carcinoma xenografts and promote tumor cell apoptosis.
authors.affiliation: Department of Obstetrics & Gynecology, Zhongshan Hospital, Xiamen University, Xiamen 361004, China. Email: mingzhu.1122@aliyun.com.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Ye
authors.affiliation: Department of Obstetrics & Gynecology, Zhongshan Hospital, Xiamen University, Xiamen 361004, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Obstetrics & Gynecology, Zhongshan Hospital, Xiamen University, Xiamen 361004, China.
authors.forename: Xiumin
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Obstetrics & Gynecology, Zhongshan Hospital, Xiamen University, Xiamen 361004, China.
authors.forename: Daying
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Obstetrics & Gynecology, Zhongshan Hospital, Xiamen University, Xiamen 361004, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D018394:CA-125 Antigen / Q000276:immunology*; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D007167:Immunotherapy; D051379:Mice; D008819:Mice, Nude; D009375:Neoplasms, Glandular and Epithelial / Q000628:therapy*; D009838:Oligodeoxyribonucleotides / Q000276:immunology*; D010051:Ovarian Neoplasms / Q000628:therapy*; D023041:Xenograft Model Antitumor Assays
chemical_list: D018394:CA-125 Antigen; D009838:Oligodeoxyribonucleotides