title: A novel animal model of osteonecrosis of the femoral head induced using a magnetic resonance imaging-guided argon-helium cryotherapy system.
abstract: The aim of the present study was to establish a novel animal model of osteonecrosis of the femoral head (ONFH) using a magnetic resonance imaging (MRI)-guided argon-helium cryotherapy system. A total of 48 rabbits were used to generate the ONFH models. In group I, the left femoral head of the rabbits received two cycles of argon-helium freezing-thawing under MRI guidance, while in group II, the right femoral head of each rabbit received only one cycle of argon-helium freezing-thawing. X-ray and histological examinations were performed. The percentages of lacunae in the femoral heads of group I at weeks 4, 8 and 12 following surgery (49.75±3.17, 62.06±4.12 and 48.25±2.76%, respectively) were higher than those in group II (39.13±4.48, 50.69±3.84 and 37.50±3.86%, respectively). In addition, the percentage of empty lacunae in group I was 62.06% at week 8 following surgery. Therefore, an animal model of ONFH was successfully established using an argon-helium cryotherapy system. The percentage of empty lacunae in group I was higher than that in group II at weeks 4, 8 and 12 after surgery.
authors.affiliation: Department of Orthopedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Shandong Jiaotong Hospital, Shandong University, Jinan, Shandong 250031, P.R. China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Interventional MRI, Shandong Medical Imaging Research Institute, Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Medical Imaging, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Interventional MRI, Shandong Medical Imaging Research Institute, Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zong
authors.affiliation: Department of Cardiology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Honglei
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Huawu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Huibo
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Jianbao
authors.initials: J
authors.lastname: Gong
authors.affiliation: Department of Orthopedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Shui
authors.initials: S
authors.lastname: Sun
keywords: animal model; cryotherapy; femoral head; magnetic resonance imaging; osteonecrosis