title: Plasma metabolomic profiling to reveal antipyretic mechanism of Shuang-huang-lian injection on yeast-induced pyrexia rats.
abstract: Shuang-huang-lian injection (SHLI) is a famous Chinese patent medicine, which has been wildly used in clinic for the treatment of acute respiratory tract infection, pneumonia, influenza, etc. The existing randomized controlled trial (RCT) studies suggested that SHLI could afford a certain anti-febrile action. However, seldom does research concern the pharmacological mechanisms of SHLI. In the current study, we explored plasma metabolomic profiling technique and selected potential metabolic markers to reveal the antipyretic mechanism of SHLI on yeast-induced pyrexia rat model using UPLC-Q-TOF/MS coupled with multivariate statistical analysis and pattern recognition techniques. We discovered a significant perturbance of metabolic profile in the plasma of fever rats and obvious reversion in SHLI-administered rats. Eight potential biomarkers, i.e. 1) 3-hydeoxybutyric acid, 2) leucine, 3) 16:0 LPC, 4) allocholic acid, 5) vitamin B2, 6) Cys-Lys-His, 7) 18:2 LPC, and 8) 3-hydroxychola-7, 22-dien-24-oic acid, were screened out by OPLS-DA approach. Five potential perturbed metabolic pathways, i.e. 1) valine, leucine, and isoleucine biosynthesis, 2) glycerophospholipid metabolism, 3) ketone bodies synthesis and degradation, 4) bile acid biosynthesis, and 5) riboflavin metabolism, were revealed to relate to the antipyretic mechanisms of SHLI. Overall, we investigated antipyretic mechanisms of SHLI at metabolomic level for the first time, and the obtained results highlights the necessity of adopting metabolomics as a reliable tool for understanding the holism and synergism of Chinese patent drug.
authors.affiliation: Science experiment center for TCM, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Gao
authors.affiliation: Science experiment center for TCM, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Guo
authors.affiliation: School of Chinese material medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Science experiment center for TCM, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Long
authors.initials: L
authors.lastname: Peng
authors.affiliation: Science experiment center for TCM, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Liu
authors.affiliation: Science experiment center for TCM, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Waters technologies (Shanghai) Ltd., Shanghai, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Bai
authors.affiliation: The 2nd Traditional Chinese Medicine factory of Harbin pharm group CO. LTD, Harbin, China.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Chinese material medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Chengke
authors.initials: C
authors.lastname: Cai
mesh_terms: D000596:Amino Acids / Q000097:blood; D000818:Animals; D058633:Antipyretics / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005227:Fatty Acids / Q000097:blood; D005334:Fever / Q000097:blood* / Q000188:drug therapy* / Q000382:microbiology / Q000503:physiopathology; D008297:Male; D055442:Metabolome; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015003:Yeasts / Q000502:physiology
chemical_list: D000596:Amino Acids; D058633:Antipyretics; D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D005227:Fatty Acids; C516400:shuang-huang-lian