title: Riboadenosine-substituted DNA probes for self-illuminating real-time monitoring of exonuclease III activity and exonuclease III-assisted target recycling.
abstract: We develop a riboadenosine-substituted DNA probe-based bioluminescence method for real-time monitoring of exonuclease III (Exo III) activity and Exo III-assisted target recycling. This method employs the target-dependent ATP as the bioluminescence reporter without the involvement of any sophisticated luciferase manipulations, and the glow-type reaction kinetics facilitates the performance of imaging assay.
authors.affiliation: Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. zhangcy@siat.ac.cn.
authors.forename: Qinfeng
authors.initials: Q
authors.lastname: Xu
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry* / Q000378:metabolism; D004789:Enzyme Activation; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D007700:Kinetics; D008163:Luminescent Measurements; D013997:Time Factors
chemical_list: D015342:DNA Probes; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D000241:Adenosine