title: Spontaneous in vitro senescence of glioma cells confirmed by an antibody against IDH1R132H.
abstract: BACKGROUND
  We have recently suggested that glioblastoma cells become spontaneously senescent in cell culture conditions. The antibody specific against IDH1(R132H) offers the perfect opportunity to verify this hypothesis.
  
  
  METHODS
  We analyzed the features of senescence in 8 glioma cell cultures showing the IDH1(R132H) mutation based on combination of immunocytochemistry, enzymo-cytochemistry, BrdU incorporation assay and real-time microscopic observation.
  
  
  RESULTS
  We report that glioma cells showing the IDH1(R132H) mutation become rapidly and spontaneously senescent in vitro. Senescence was observed in both classical and novel serum-free cell culture conditions. Importantly, the senescent IDH1(R132H)-positive cells showed the expression of stemness marker (SOX2).
  
  
  CONCLUSIONS
  In vitro senescence appeared to be the main reason of the difficulties in any kind culturing of glioma cells. 3D cell cultures prolonged the survival and in vitro proliferation of neoplastic IDH1(R132H)-positive cells, however, did not enhance the stabilization efficiency. Senescence of glioma cells is spontaneously triggered in vitro, which offers the opportunity of potential new therapeutic strategies based on this phenomenon.
authors.affiliation: Department of Tumor Biology, Medical University of Lodz, Lodz, Poland ewelina.stoczynska-fidelus@umed.lodz.pl.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Stoczynska-Fidelus
authors.affiliation: Clinical Department of Neurosurgery, The Voivodal Specialistic Hospital in Olsztyn, Olsztyn, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Och
authors.affiliation: Department of Tumor Biology, Medical University of Lodz, Lodz, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Rieske
authors.affiliation: Department of Molecular Pathology and Neuropathology, Chair of Oncology, Medical University of Lodz, Lodz, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Bienkowski
authors.affiliation: Department of Tumor Biology, Medical University of Lodz, Lodz, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Banaszczyk
authors.affiliation: Department of Tumor Biology, Medical University of Lodz, Lodz, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Winiecka-Klimek
authors.affiliation: Department of Tumor Biology, Medical University of Lodz, Lodz, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Zieba
authors.affiliation: Department of Tumor Biology, Medical University of Lodz, Lodz, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Janik
authors.affiliation: Department of Tumor Biology, Medical University of Lodz, Lodz, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Rosiak
authors.affiliation: Department of Tumor Biology, Medical University of Lodz, Lodz, Poland.
authors.forename: Cezary
authors.initials: C
authors.lastname: Treda
authors.affiliation: Department of Clinical Physiology, Medical University of Lodz, Lodz, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Stawski
authors.affiliation: Neurological Surgery, The Maria Sklodowska-Curie Regional Specialist Hospital in Zgierz, Zgierz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Radomiak-Zaluska
authors.affiliation: Department of Tumor Biology, Medical University of Lodz, Lodz, Poland.
authors.forename: Sylwester
authors.initials: S
authors.lastname: Piaskowski
mesh_terms: D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D018929:Cell Culture Techniques; D002465:Cell Movement; D049109:Cell Proliferation; D016922:Cellular Senescence / Q000502:physiology*; D005434:Flow Cytometry; D005910:Glioma / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human
keywords: 3D cell culture; IDH1 gene; astrocytoma; glioblastoma; glioma; senescence