title: [Effects of catalpol, L-shikonin and paeonol extracted from radix rehmanniae, radix arnebiae and cortex moutan on KGF-induced HaCaT cell proliferation].
abstract: OBJECTIVE
  To explore the effects of Chinese herbal medicine radix rehmanniae, radix arnebiae and cortex moutan on the proliferation of HaCaT cells and explore their potential curative mechanisms.
  
  
  METHODS
  The main monomers of catalpol, l-shikonin and paeonol were extracted. And 10 ng/ml keratinocyte growth factor (KGF) was used to induce HaCaT cell to build an in vitro model of hyperproliferation of epidermal keratinocytes. CCK-8 assay and flow cytometry were applied to examine the effects of herbal monomers on cell proliferation and cell cycle.
  
  
  RESULTS
  Both l-shikonin ( ≥ 10(-6) mol/L) and paeonol ( ≥ 1.88×10(-4) mol/L) inhibited cell proliferation while catalpol ( ≥ 10(-6) mol/L) enhanced cell proliferation.L-shikonin ( ≥ 10(-6) mol/L) and paeonol ( ≥ 1.88×10(-4) mol/L) inhibited the HaCaT cell during S and G2M phases while catalpol ( ≥ 10(-6) mol/L) enhanced HaCaT cell during S phase but not G2M phase.
  
  
  CONCLUSIONS
  L-shikonin and paeonol inhibits the proliferation of HaCaT cells while catalpol has opposite effects.
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Lü
authors.forename: Yuling
authors.initials: Y
authors.lastname: Wang
authors.forename: Wenbin
authors.initials: W
authors.lastname: Zhao
authors.forename: Nuo
authors.initials: N
authors.lastname: Li
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.forename: Jianxiang
authors.initials: J
authors.lastname: Lu
authors.forename: Weixian
authors.initials: W
authors.lastname: Zeng
authors.forename: Shentao
authors.initials: S
authors.lastname: Bao
authors.affiliation: Department of Dermatology, China-Japan Friendship Hospital,Beijing 100029, China. Email: zhi@tsinghua.edu.cn.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Bai
mesh_terms: D000098:Acetophenones / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D057888:Iridoid Glucosides / Q000494:pharmacology*; D009285:Naphthoquinones / Q000494:pharmacology*
chemical_list: D000098:Acetophenones; D057888:Iridoid Glucosides; D009285:Naphthoquinones; C078040:catalpol; C016101:shikonin; C013638:paeonol