title: Spectroscopy and docking simulations of the interaction between lochnericine and bovine serum albumin.
abstract: Lochnericine (LOC) is a component of Voacanga africana, which is a type of traditional medical food in Africa widely used for treating diseases. In this article, the interaction between LOC and bovine serum albumin (BSA) was studied by fluorescence spectroscopy. Furthermore, Fourier transform infrared (FTIR), Raman and circular dichroism (CD) were used to investigate the structural changes of BSA. The experimental results consistently indicated that LOC changed the secondary structure of BSA. Three structure-similar components were used to study the interference experiments. The molecular modeling results showed that LOC could bind within not only sites I and II, but also bind the cavity of subdomain IB.
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Jiawei
authors.initials: J
authors.lastname: He
authors.forename: Jin
authors.initials: J
authors.lastname: Yan
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D026121:Indole Alkaloids / Q000737:chemistry*; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D046948:Secologanin Tryptamine Alkaloids; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D026121:Indole Alkaloids; D046948:Secologanin Tryptamine Alkaloids; D012710:Serum Albumin, Bovine; C582596:lochnericine
keywords: bovine serum albumin (BSA); interaction; lochnericine; molecular modeling; structural changes