title: Human papillomavirus genotype distribution and E6/E7 oncogene expression in Turkish women with cervical cytological findings.
abstract: BACKGROUND
  Infection with certain human papillomavirus (HPV) genotypes is the most important risk factor related with cervical cancer. The objective of the present study was to investigate the prevalence of HPV infection, the distribution of HPV genotypes and HPV E6/E7 oncogene mRNA expression in Turkish women with different cervical cytological findings in Mersin province, Southern Turkey.
  
  
  METHODS
  A total of 476 cytological samples belonging to women with normal and abnormal cervical Pap smears were enrolled in the study. For the detection and genotyping assay, a PCR/direct cycle sequencing approach was used. E6/E7 mRNA expression of HPV-16, 18, 31, 33, and 45 was determined by type-specific real-time NASBA assay (NucliSENS EasyQ(®)HPV v1.1).
  
  
  RESULTS
  Of the 476 samples, 106 (22.3%) were found to be positive for HPV DNA by PCR. The presence of HPV was significantly more common (p<0.001) in HSIL (6/8, 75%) when compared with LSIL (6/14, 42.9%), ASC-US (22/74, 29.7%) and normal cytology (72/380, 18.9%). The most prevalent genotypes were, in descending order of frequency, HPV genotype 66 (22.6%), 16 (20.8%), 6 (14.2%), 31 (11.3%), 53 (5.7%), and 83 (4.7%). HPV E6/E7 oncogene mRNA positivity (12/476, 2.5%) was lower than DNA positivity (38/476, 7.9%).
  
  
  CONCLUSIONS
  Our data present a wide distribution of HPV genotypes in the analyzed population. HPV genotypes 66, 16, 6, 31, 53 and 83 were the predominant types and most of them were potential carcinogenic types. Because of the differences between HPV E6/E7 mRNA and DNA positivity, further studies are required to test the role of mRNA testing in the triage of women with abnormal cervical cytology or follow up of HPV DNA positive and cytology negative. These epidemiological data will be important to determine the future impact of vaccination on HPV infected women in our region.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Faculty of Pharmacy, Mersin University, Mersin, Turkey E-mail : tezcanseda@yahoo.com, tezcanseda@mersin.edu.tr.
authors.forename: Seda
authors.initials: S
authors.lastname: Tezcan
authors.forename: Didem
authors.initials: D
authors.lastname: Ozgur
authors.forename: Mahmut
authors.initials: M
authors.lastname: Ulger
authors.forename: Gonul
authors.initials: G
authors.lastname: Aslan
authors.forename: Iclal
authors.initials: I
authors.lastname: Gurses
authors.forename: Mehmet Sami
authors.initials: MS
authors.lastname: Serin
authors.forename: Burcu Gurer
authors.initials: BG
authors.lastname: Giray
authors.forename: Saffet
authors.initials: S
authors.lastname: Dilek
authors.forename: Gurol
authors.initials: G
authors.lastname: Emekdas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000166:cytology / Q000473:pathology*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D058952:Human papillomavirus 31 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D030361:Papillomavirus Infections / Q000821:virology; D012333:RNA, Messenger / Q000096:biosynthesis; D012097:Repressor Proteins / Q000235:genetics; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000473:pathology* / Q000821:virology; D014421:Turkey; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology; D055815:Young Adult
chemical_list: D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16