title: Actinophytocola gilvus sp. nov., isolated from desert soil crusts, and emended description of the genus Actinophytocola Indananda et al. 2010.
abstract: An actinomycete strain, designated I12A-02593(T), was isolated from a desert soil crust sample collected in the Shapotou region of Tengger Desert, north-west China. The isolate grew well on International Streptomyces Project (ISP) media 2, 3, 5 and 7, YS and Bennett's agar; it produced spherical bodies and formed clumps on the aerial mycelia on ISP 5 agar plates. Chemotaxonomically, the strain contained meso-diaminopimelic acid as the diagnostic diamino acid, arabinose and galactose as the diagnostic sugars in whole-cell hydrolysates, MK-9(H4) as the sole isoprenoid quinone, and iso-C16 : 0, iso-C16 : 0 2-OH and iso-C16 : 1 H as the major cellular fatty acids, without mycolic acids. The profile of the phospholipids mainly comprised diphosphatidylglycerol, phosphatidylethanolamine and hydroxyphosphatidylethanolamine. The genomic DNA G+C content was 70.1 mol%. The 16S rRNA gene sequence of strain I12A-02593(T) exhibited 96.4-97.4 % similarities with members of the genus Actinophytocola. In the phylogenetic tree based on 16S rRNA gene sequences, the isolate formed a robust cluster with Actinophytocola oryzae NBRC 105245(T), Aactinophytocola timorensis NBRC 105524(T), Actinophytocola corallina NBRC 105525(T), Actinophytocola burenkhanensis NBRC 105883(T)and Actinophytocola xinjiangensis NBRC 106673(T). DNA-DNA hybridization values between strain I12A-02593(T) and the five species of the genus Actinophytocola were all less than 70 %. On the basis of the polyphasic taxonomy evidence, a novel species of the genus Actinophytocola is proposed, with the name Actinophytocola gilvus sp. nov. The type strain is I12A-02593(T) ( = CPCC 203543(T) = DSM 45828(T) = NBRC 109453(T) = KCTC 29165(T)). An emended description of the genus Actinophytocola is also provided.
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Sun
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Yu
authors.affiliation: Department of Biological Sciences, Kent State University, Kent, Ohio 44240, USA.
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Lu
authors.affiliation: Department of Biological Sciences, Kent State University, Kent, Ohio 44240, USA.
authors.forename: Xiao-Zhen
authors.initials: XZ
authors.lastname: Mou
authors.affiliation: Department of Biological Sciences, Kent State University, Kent, Ohio 44240, USA.|Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003889:Desert Climate; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid