title: Structural and thermodynamic characterization of the recognition of the S100-binding peptides TRTK12 and p53 by calmodulin.
abstract: Calmodulin (CaM) is a multifunctional messenger protein that activates a wide variety of signaling pathways in eukaryotic cells in a calcium-dependent manner. CaM has been proposed to be functionally distinct from the S100 proteins, a related family of eukaryotic calcium-binding proteins. Previously, it was demonstrated that peptides derived from the actin-capping protein, TRTK12, and the tumor-suppressor protein, p53, interact with multiple members of the S100 proteins. To test the specificity of these peptides, they were screened using isothermal titration calorimetry against 16 members of the human S100 protein family, as well as CaM, which served as a negative control. Interestingly, both the TRTK12 and p53 peptides were found to interact with CaM. These interactions were further confirmed by both fluorescence and nuclear magnetic resonance spectroscopies. These peptides have distinct sequences from the known CaM target sequences. The TRTK12 peptide was found to independently interact with both CaM domains and bind with a stoichiometry of 2:1 and dissociations constants Kd,C-term  = 2 ± 1 µM and Kd,N-term  = 14 ± 1 µM. In contrast, the p53 peptide was found to interact only with the C-terminal domain of CaM, Kd,C-term = 2 ± 1 µM, 25°C. Using NMR spectroscopy, the locations of the peptide binding sites were mapped onto the structure of CaM. The binding sites for both peptides were found to overlap with the binding interface for previously identified targets on both domains of CaM. This study demonstrates the plasticity of CaM in target binding and may suggest a possible overlap in target specificity between CaM and the S100 proteins.
authors.affiliation: Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, 12180; Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York, 12180.
authors.forename: Lucas N
authors.initials: LN
authors.lastname: Wafer
authors.forename: Franco O
authors.initials: FO
authors.lastname: Tzul
authors.forename: Pranav P
authors.initials: PP
authors.lastname: Pandharipande
authors.forename: Scott A
authors.initials: SA
authors.lastname: McCallum
authors.forename: George I
authors.initials: GI
authors.lastname: Makhatadze
mesh_terms: D002147:Calmodulin / Q000737:chemistry / Q000378:metabolism*; D051347:CapZ Actin Capping Protein / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D009418:S100 Proteins / Q000378:metabolism*; D013816:Thermodynamics; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002147:Calmodulin; D051347:CapZ Actin Capping Protein; D010446:Peptide Fragments; D009418:S100 Proteins; C475482:TRTK-12 peptide; D016159:Tumor Suppressor Protein p53
keywords: CapZ; S100 proteins; TRTK12; calmodulin; isothermal titration calorimetry; line-shape analysis; nuclear magnetic resonance; p53