title: Activation of extrinsic- and Daxx-mediated pathways in lymphoid tissue of PRRSV-infected pigs.
abstract: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is a major infectious pathogen in pigs leading to huge economical losses worldwide. PRRSV is able to escape from host immunity and causes transient infections. In the present study, expression of different apoptotic markers and its connection with PRRSV were assessed in tonsil and mediastinal lymph node from PRRSV-infected pigs. Cleaved caspase (CCasp)8, CCasp9, Fas, Daxx, CCasp3 and PRRSV expression were analyzed by immunohistochemistry. An up-regulation of CCasp8, Fas and CCasp3 expression in lymphocytes and macrophages from both organs was found during PRRSV infection, indicating the activation of the extrinsic-mediated pathway of apoptosis. Moreover, Daxx expression was also enhanced in macrophages of both organs, suggesting a simultaneous caspase-independent pathway of apoptosis. A correlation between the expression of the different apoptotic markers and IL-10, IL-6 and TGF-β but not with PRRSV antigen was found in our study, which supports the hypothesis of an indirect mechanism in PRRSV-induced apoptosis.
authors.affiliation: Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, Córdoba University, "International Excellence Agrifood Campus - CeiA3", 14014 Córdoba, Spain. Electronic address: v22rogoi@uco.es.
authors.forename: Irene M
authors.initials: IM
authors.lastname: Rodríguez-Gómez
authors.affiliation: Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, Córdoba University, "International Excellence Agrifood Campus - CeiA3", 14014 Córdoba, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Barranco
authors.affiliation: Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, Córdoba University, "International Excellence Agrifood Campus - CeiA3", 14014 Córdoba, Spain.
authors.forename: Shyrley P
authors.initials: SP
authors.lastname: Amarilla
authors.affiliation: Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, Murcia University, "Mare Nostrum Campus of Excellence - 37 38", 30100 Murcia, Spain.
authors.forename: Obdulio
authors.initials: O
authors.lastname: García-Nicolás
authors.affiliation: School of Veterinary Medicine, Faculty of Health & Medical Sciences, Surrey University, Guildford, Surrey, GU2 7TE, United Kingdom.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Salguero
authors.affiliation: Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, Córdoba University, "International Excellence Agrifood Campus - CeiA3", 14014 Córdoba, Spain.
authors.forename: Librado
authors.initials: L
authors.lastname: Carrasco
authors.affiliation: CICAP - Agrifood Research Centre, 14400 Pozoblanco, Córdoba, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Gómez-Laguna
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism / Q000821:virology; D008214:Lymphocytes / Q000378:metabolism / Q000821:virology; D008264:Macrophages / Q000378:metabolism / Q000821:virology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D014066:Palatine Tonsil / Q000378:metabolism / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D015398:Signal Transduction; D013552:Swine; D016212:Transforming Growth Factor beta / Q000378:metabolism; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015850:Interleukin-6; D009687:Nuclear Proteins; D016212:Transforming Growth Factor beta; D019014:fas Receptor; D016753:Interleukin-10; D020169:Caspases
keywords: Apoptosis; Caspases; Cytokines; Daxx; Fas; PRRSV