title: Monoamine neurotransmitters and fibroblast growth factor-2 in the brains of rats with post-stroke depression.
abstract: The aim of the present study was to investigate the changes in the levels of serotonin (5-HT), dopamine (DA), norepinephrine (NE) and fibroblast growth factor-2 (FGF-2) in the brains of rats with post-stroke depression (PSD). A rat model of stroke was established by middle cerebral artery occlusion and the rats were randomly divided into two groups: Control and modification groups. The rats in the modification group had PSD, while the rats in the control group had experienced a stroke only. The PSD model was established by applying chronic mild stress to the individually housed rats. High-performance liquid chromatography was used to detect the levels of 5-HT, DA and NE, while western blotting was used to detect the FGF-2 protein expression levels in the frontal lobe and hippocampus. Quantitative polymerase chain reaction was also used to determine the mRNA expression levels of FGF-2 in the frontal lobes of the two groups. The levels of 5-HT, DA and NE in the frontal lobe and hippocampus of the rats in the PSD group were significantly lower than the levels observed in the rats in the stroke group (P<0.01). In addition, protein expression levels of FGF-2 in the frontal lobe of the rats in the PSD group were significantly lower when compared with the control group rats (P<0.01), however, the protein expression levels of FGF-2 in the hippocampus did not exhibit a statistically significant difference (P>0.05). The mRNA expression levels of FGF-2 in the frontal lobe of the rats in the modification group were significantly lower than the levels in the control group rats (P<0.01). Therefore, reduced levels of monoamine neurotransmitters and FGF-2 expression in the brains of rats with PSD are associated with the incidence of PSD.
authors.affiliation: Department of Neurology, Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250001, P.R. China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Ji
authors.affiliation: Blood Purification Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China.
authors.forename: Chun-Ling
authors.initials: CL
authors.lastname: Wu
authors.affiliation: Department of Neurology, Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250001, P.R. China.
authors.forename: Xing-Chen
authors.initials: XC
authors.lastname: Wang
authors.affiliation: Department of Neurology, Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250001, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Bi
authors.affiliation: Department of Traditional Chinese Medicine, Dongying People's Hospital, Dongying, Shandong 257091, P.R. China.
authors.forename: Dian-Yun
authors.initials: DY
authors.lastname: Wang
keywords: dopamine; fibroblast growth factor-2; norepinepherine; post-stroke depression rat; serotonin