title: Papiliotrema siamense f.a., sp. nov., a yeast species isolated from plant leaves.
abstract: Two strains representing a novel species were isolated from the external surface of a sugar cane leaf (DMKU-SP85(T)) and tissue of a rice leaf (DMKU-RE97) collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, the two strains were determined to represent a novel species of the genus Papiliotrema although sexual reproduction was not observed. The sequences of the D1/D2 region of the LSU rRNA gene and ITS region of the two strains were identical, but differed from those of the type strain of Cryptococcus nemorosus by 0.6 % nucleotide substitutions (four nucleotide substitutions out of 597 nucleotides) in the D1/D2 region of the LSU rRNA gene and 1.8 % nucleotide substitutions (nine nucleotide substitutions out of 499 nucleotides) in the ITS region. The name Papiliotrema siamense f.a., sp. nov. is proposed. The type strain is DMKU-SP85(T)( = BCC 69499(T) = CBS 13330(T)).
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Janjira
authors.initials: J
authors.lastname: Surussawadee
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Pannida
authors.initials: P
authors.lastname: Khunnamwong
authors.affiliation: Center for Advanced Studies in Tropical Natural Resources, National Research University, Kasetsart University, Thailand.|Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Nantana
authors.initials: N
authors.lastname: Srisuk
authors.affiliation: Center for Advanced Studies in Tropical Natural Resources, National Research University, Kasetsart University, Thailand.|Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Savitree
authors.initials: S
authors.lastname: Limtong
mesh_terms: D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D031786:Saccharum / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer