title: Smed-dynA-1 is a planarian nervous system specific dynamin 1 homolog required for normal locomotion.
abstract: Dynamins are GTPases that are required for separation of vesicles from the plasma membrane and thus are key regulators of endocytosis in eukaryotic cells. This role for dynamin proteins is especially crucial for the proper function of neurons, where they ensure that synaptic vesicles and their neurotransmitter cargo are recycled in the presynaptic cell. Here we have characterized the dynamin protein family in the freshwater planarian Schmidtea mediterranea and showed that it possesses six dynamins with tissue specific expression profiles. Of these six planarian homologs, two are necessary for normal tissue homeostasis, and the loss of another, Smed-dynA-1, leads to an abnormal behavioral phenotype, which we have quantified using automated center of mass tracking. Smed-dynA-1 is primarily expressed in the planarian nervous system and is a functional homolog of the mammalian Dynamin I. The distinct expression profiles of the six dynamin genes makes planarians an interesting new system to reveal novel dynamin functions, which may be determined by their differential tissue localization. The observed complexity of neurotransmitter regulation combined with the tools of quantitative behavioral assays as a functional readout for neuronal activity, renders planarians an ideal system for studying how the nervous system controls behavior.
authors.affiliation: Lewis-Sigler Institute for Integrative Genomics, Carl C. Icahn Laboratory, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Jared A
authors.initials: JA
authors.lastname: Talbot
authors.affiliation: Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
authors.forename: Ko W
authors.initials: KW
authors.lastname: Currie
authors.affiliation: Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada Ontario Institute for Cancer Research, Toronto, ON M5G 0A3, Canada.
authors.forename: Bret J
authors.initials: BJ
authors.lastname: Pearson
authors.affiliation: Lewis-Sigler Institute for Integrative Genomics, Carl C. Icahn Laboratory, Princeton University, Princeton, NJ 08544, USA Physics Department, University of California at San Diego, La Jolla, CA 92093, USA Division of Cell and Developmental Biology, University of California at San Diego, La Jolla, CA 92093, USA emscollins@physics.ucsd.edu.
authors.forename: Eva-Maria S
authors.initials: EM
authors.lastname: Collins
keywords: Behavior; Dynamin; Locomotion; Planarian