title: Maturation of mouse bone marrow dendritic cells (BMDCs) induced by Laminaria japonica polysaccharides (LJP).
abstract: The seaweed Laminaria japonica has been investigated in a laboratory research for its medical significance and LJP has been purified now. The objective of present study was to look at effect of LJP on structural, phenotypic and functional maturation of murine BMDCs. The structural maturation of BMDCs induced by LJP was evaluated by transmission electron microscopy (TEM); The phenotypic maturation of BMDCs was studied by flow cytometry(FCM) and functional maturation of BMDCs was analyzed by FITC-dextran, acid phosphatase (ACP) activity and enzyme linked immunosorbent assay (ELISA). We hereby proved that LJP markedly induced maturation of BMDCs with the data of decreased the number of lysosomes, upregulated expression of CD80, CD83, CD86, CD40 and MHC II key membrane molecules on BMDCs, downregulated phagocytosis, enriched production of IL-12 and TNF-α secreted by BMDCs. Therefore it should be concluded that LJP was with strong ability to induce maturation of BMDCs. Our data provided direct evidence to suggest that LJP could be considered as an immune stimulant in improving immune handicapped situation and as a useful adjuvant in vaccine designing.
authors.affiliation: Experimental and Technical Center, China Medical University, No. 92, North Second Road, Shenyang 110001, China.
authors.forename: Jingjuan
authors.initials: J
authors.lastname: Meng
authors.affiliation: Department of Immunology, School of Basic Medical Science, China Medical University, No. 92, North Second Road, Shenyang 110001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Immunology, School of Basic Medical Science, China Medical University, No. 92, North Second Road, Shenyang 110001, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Meng
authors.affiliation: College of Medical Laboratory, Dalian Medical University, Dalian 11604, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Dermatology, No. 1 Hospital, China Medical University, Shenyang 110001, China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Immunology, School of Basic Medical Science, China Medical University, No. 92, North Second Road, Shenyang 110001, China. Electronic address: fpshan@mail.cmu.edu.cn.
authors.forename: Fengping
authors.initials: F
authors.lastname: Shan
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D007155:Immunologic Factors / Q000494:pharmacology*; D018664:Interleukin-12 / Q000096:biosynthesis; D007795:Laminaria / Q000737:chemistry*; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D007155:Immunologic Factors; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D000135:Acid Phosphatase
keywords: Bone marrow derived dendritic cells; Immunoregulation; Laminaria japonica polysaccharides; Maturation