title: Comprehensive identification of active triterpenoid metabolites in frankincense using a coupling strategy.
abstract: Frankincense resins are extensively used as natural remedies in regions ranging from North Africa to China. Triterpenoid metabolites from frankincense exhibit notable anti-inflammatory and anti-tumor properties. In the present paper, without the use of an isolation process, the fragmentation rules and NMR spectral characteristics of triterpenoid metabolites in frankincense are summarized through a coupling method using high performance liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MS(n)) combined with HPLC-nuclear magnetic resonance (NMR) experiments. Based on this groundwork, a coupling strategy for the comprehensive metabolic profiling of active triterpenoid metabolites from enriched fractions of frankincense was developed. The proposed strategy may serve as a method for the holistic screening of bioactive metabolites in complex TCM samples.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China. Electronic address: yyliu_1980@163.com.
authors.forename: Yuanyan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China. Electronic address: zhenli_liu@sina.com.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Jianbei
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China; Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Zhangchi
authors.initials: Z
authors.lastname: Ning
authors.affiliation: Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Song
authors.affiliation: Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Du
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Lu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, China; Institute for Advancing Translational Medicine in Bone & Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China. Electronic address: lap64067611@126.com.
authors.forename: Aiping
authors.initials: A
authors.lastname: Lu
mesh_terms: D029743:Boswellia / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D065260:Frankincense / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D053719:Tandem Mass Spectrometry / Q000379:methods; D013729:Terpenes / Q000032:analysis* / Q000378:metabolism
chemical_list: D013729:Terpenes; D065260:Frankincense
keywords: Frankincense resins; Mass spectrometry; Metabolic profiling; Nuclear magnetic resonance; Triterpenoid metabolites