title: Cell-cycle analysis and apoptosis-associated proteins in cervical lesions of Brazilian women.
abstract: OBJECTIVE
  The aim of the present study was to detect the relative expressions of p53, p21(Waf1/Cip1), p27(Kip1) Bcl-2 and cleaved caspase-3 in cervical lesion samples from Brazilian women by immunohistochemistry.
  
  
  METHODS
  A total of 230 cervical biopsies in paraffin-embedded blocks were studied: 43 were invasive squamous cell carcinomas (SCC), 52 carcinomas in situ/cervical intraepithelial neoplasias III (CIN III), 54 cervical intraepithelial neoplasias II (CIN II), 51 cervical intraepithelial neoplasias I (CIN I) and 30 non-neoplastic lesions (NN) with benign cellular changes.
  
  
  RESULTS
  Significant differences were observed in the p53 expression between the different groups: NN and CIN I (p=0.010); NN and CIN II (p<0.00001); CIN II and CIN III (p=0.02); CIN II and CIS (p=0.0220); CIN II and CEC (p=0.010). Regarding p21(WAF1/Cip1), significant differences were observed between NN and CEC (p=0.001); CIN I and CEC (p=0.001); CIN II and CIN III (p=0,001); CIN II and CIS (p=0.0004) and CIN II and CEC (p<0.0001). For p27(Kip1), significant differences were observed between NN and CIN I (p<0.00001); NN and CIN II (p<0.00001); NN and CIS (p=0.038); CIN I and CIN III (p=0.001); CIN I and CIS (p=0.009); CIN I and CEC (p=0.0001); CIN II and CIN III (p=0.0003); CIN II and CIS (p=0.002); CIN II and CEC (p< 0.00001). Bcl-2 and caspase-3 did not show remarkable differences between groups.
  
  
  CONCLUSIONS
  p53, p21(WAF1/CIP1), p27(KIP1) appear to be involved in the course of carcinogenesis. Rare expression of Bcl-2 and cleaved caspase-3 suggests that these proteins probably do not participate in cervical apoptosis.
authors.affiliation: Department of Pathology, Federal University of São Paulo, UNIFESP, SP, Brazil.
authors.forename: Cristine
authors.initials: C
authors.lastname: Dobo
authors.affiliation: Department of Pathology, Federal University of São Paulo, UNIFESP, SP, Brazil.
authors.forename: Celina Tizuko Fujiyama
authors.initials: CT
authors.lastname: Oshima
authors.affiliation: Department of Pathology, Federal University of São Paulo, UNIFESP, SP, Brazil.
authors.forename: Flávio
authors.initials: F
authors.lastname: De Oliveira Lima
authors.affiliation: Department of Pathology, Federal University of São Paulo, UNIFESP, SP, Brazil.
authors.forename: Thiago Simão
authors.initials: TS
authors.lastname: Gomes
authors.affiliation: Department of Pathology, Federal University of São Paulo, UNIFESP, SP, Brazil.
authors.forename: João Norberto
authors.initials: JN
authors.lastname: Stávale
authors.affiliation: Department of Pathology, Federal University of São Paulo, UNIFESP, SP, Brazil.
authors.forename: Vítor
authors.initials: V
authors.lastname: Arias
authors.affiliation: Department of Pathology, Federal University of São Paulo, UNIFESP, SP, Brazil daribeiro@unifesp.br ak92@hotmail.com.
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
authors.affiliation: Department of Pathology, Federal University of São Paulo, UNIFESP, SP, Brazil.
authors.forename: Gustavo R A
authors.initials: GR
authors.lastname: Focchi
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001938:Brazil; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000473:pathology
chemical_list: D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; C493956:CDKN1A protein, human; C436291:CDKN1B protein, human; D018797:Cell Cycle Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D050760:Cyclin-Dependent Kinase Inhibitor p27; D053148:Caspase 3
keywords: Cervical cancer; apoptosis; bcl-2; caspase-3; p21WAF1/Cip1; p27Kip1; p53