title: Newly-synthesized chalcones-inhibition of adherence and biofilm formation of methicillin-resistant Staphylococcus aureus.
abstract: Biofilm formation and adherence of bacteria to host tissue are one of the most important virulence factors of methicillin-resistant strains of Staphylococcus aureus (MRSA). The number of resistant strains is seriously increasing during the past years and bacteria have become resistant, not only to methicillin, but also to other commonly used antistaphylococcal antibiotics. There is a great need for discovering a novel antimicrobial agent for the treatment of staphylococcal infections. One of the most promising groups of compounds appears to be chalcones. In present study we evaluated the in vitro effect of three newly synthesized chalcones: 1,3- Bis-(2-hydroxy-phenyl)-propenone, 3-(3-Hydroxy-phenyl)-1-(2-hydroxy-phenyl)-propenone and 3-(4-Hydroxy-phenyl)-1-(2-hydroxy-phenyl)-propenone on glycocalyx production, biofilm formation and adherence to human fibronectin of clinical isolates and laboratory control strain of MRSA (ATCC 43300). Subinhibitory concentrations of the tested compounds reduced the production of glycocalyx, biofilm formation and adherence to human fibronectin of all MRSA strains. Inhibition of biofilm formation was dose dependent and the most effective was 1,3- Bis-(2-hydroxy-phenyl)-propenone. In our study we demonstrated that three newly-synthesized chalcones exhibited significant effect on adherence and biofilm formation of MRSA strains. Chalcones may be considered as promising new antimicrobial agents that can be used for prevention of staphylococcal infections or as adjunct to antibiotics in conventional therapy.
authors.affiliation: Department of Microbiology and Immunology University of Belgrade-Faculty of Pharmacy Belgrade Serbia.
authors.forename: Dragana D
authors.initials: DD
authors.lastname: Bozic
authors.affiliation: Department of Microbiology and Immunology University of Belgrade-Faculty of Pharmacy Belgrade Serbia.
authors.forename: Marina
authors.initials: M
authors.lastname: Milenkovic
authors.affiliation: Department of Pharmaceutical Chemistry University of Belgrade-Faculty of Pharmacy Belgrade Serbia.
authors.forename: Branka
authors.initials: B
authors.lastname: Ivkovic
authors.affiliation: Institute of Microbiology and Immunology School of Medicine University of Belgrade Belgrade Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Cirkovic
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D047188:Chalcones / Q000138:chemical synthesis / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins / Q000378:metabolism; D019276:Glycocalyx / Q000378:metabolism; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D013203:Staphylococcal Infections / Q000382:microbiology; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D047188:Chalcones; D005353:Fibronectins
keywords: MRSA; biofilms; chalcones; glycocalyx