title: Proteomic Analysis between U87MG and U343MG-A Cell Lines: Searching for Candidate Proteins for Glioma Invasion.
abstract: BACKGROUND
  To investigate the molecular basis for invasion of malignant gliomas, proteomic analysis approach was carried out using two human glioma cell lines, U87MG and U343MG-A that demonstrate different motility and invasiveness in in vitro experiments.
  
  
  METHODS
  High-resolution two-dimensional gel electrophoresis and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry analysis were performed.
  
  
  RESULTS
  Nine distinct protein spots that were recognized with significant alteration between the two cell lines. Five of these protein spots were up-regulated in U87MG and four were up-regulated in U343MG-A.
  
  
  CONCLUSIONS
  Among these proteins, cathepsin D was shown to be one of the important proteins which are related with glioma invasion. However, further studies are necessary to reveal the exact role and mechanism of cathepsin D in glioma invasion.
authors.affiliation: Brain Tumor Research Laboratory, Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Korea. ; Department of Neurosurgery, Worker's Hospital of Tangshan, Tangshan City, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Pei
authors.affiliation: Brain Tumor Research Laboratory, Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Korea.
authors.forename: Kyung-Sub
authors.initials: KS
authors.lastname: Moon
authors.affiliation: Department of Chemistry, College of Life Science, Chonnam National University, Gwangju, Korea.
authors.forename: SangO
authors.initials: S
authors.lastname: Pan
authors.affiliation: Brain Tumor Research Laboratory, Department of Pathology, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Korea.
authors.forename: Kyung-Hwa
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Brain Tumor Research Laboratory, Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Korea.
authors.forename: Hyang-Hwa
authors.initials: HH
authors.lastname: Ryu
authors.affiliation: Brain Tumor Research Laboratory, Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Korea.
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Jung
authors.affiliation: Brain Tumor Research Laboratory, Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Korea.
authors.forename: In-Young
authors.initials: IY
authors.lastname: Kim
authors.affiliation: Brain Tumor Research Laboratory, Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Korea.
authors.forename: Woo-Yeol
authors.initials: WY
authors.lastname: Jang
authors.affiliation: Department of Chemistry, College of Life Science, Chonnam National University, Gwangju, Korea.
authors.forename: Chae-Hun
authors.initials: CH
authors.lastname: Jung
authors.affiliation: Brain Tumor Research Laboratory, Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Korea.
authors.forename: Shin
authors.initials: S
authors.lastname: Jung
keywords: Cathepsin D; Gliomas; Invasion; Motility; Protein; Proteomics