title: Promoter characterization and functional association with placenta of porcine MAGEL2.
abstract: MAGEL2 (melanoma antigen-like gene 2) is essential for circadian function, metabolism and reproduction in mammals. This study was conducted to investigate transcriptional regulation and functional importance in the placenta of porcine MAGEL2. Quantitative real-time PCR showed that MAGEL2 was highly expressed in porcine hypothalamus, pituitary and placenta (P<0.05). The gene was down-regulated in Meishan but up-regulated in Duroc placentas from 25 days post-coitum (dpc) to 105 dpc (P<0.01). Dual luciferase assay demonstrated that the region -151/+110 had the highest promoter activity. Of the g. -712C>G and g. -708T>C polymorphisms in MAGEL2 promoter, -712C and -708T were observed to be predominant in Large White, Landrace and Duroc populations, while -712G and -708C were predominant in Meishan and Rongchang populations. Moreover, -712C>G and -708T>C had significant effects on MAGEL2 transcription (P<0.05) and placental efficiency (P<0.01). In conclusion, -151/+110 harbors the basal promoter of porcine MAGEL2. The region upstream the basal promoter contains repressive cis-elements. And, MAGEL2 is essential in porcine placenta.
authors.affiliation: Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization, Southwest University, Chongqing 400716, PR China. Electronic address: caodejiang@hotmail.com.
authors.forename: Caode
authors.initials: C
authors.lastname: Jiang
authors.affiliation: School of Life Science, Southwest University, Chongqing 400715, PR China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Life Science, Southwest University, Chongqing 400715, PR China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Huang
authors.affiliation: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom.
authors.forename: Bruce
authors.initials: B
authors.lastname: Whitelaw
mesh_terms: D000818:Animals; D001483:Base Sequence; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007031:Hypothalamus / Q000378:metabolism; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D047908:Intracellular Signaling Peptides and Proteins; D064267:Intrinsically Disordered Proteins
chemical_list: D004247:DNA; D011506:Proteins; C510822:MAGEL2 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D064267:Intrinsically Disordered Proteins
keywords: Association; MAGEL2; Pig; Placental traits; Promoter