title: Understanding pathogenic Burkholderia glumae metabolic and signaling pathways within rice tissues through in vivo transcriptome analyses.
abstract: Burkholderia glumae is a causal agent of rice grain and sheath rot. Similar to other phytopathogens, B. glumae adapts well to the host environment and controls its biology to induce diseases in the host plant; however, its molecular mechanisms are not yet fully understood. To gain a better understating of the actual physiological changes that occur in B. glumae during infection, we analyzed B. glumae transcriptome from infected rice tissues using an RNA-seq technique. To accomplish this, we analyzed differentially expressed genes (DEGs) and identified 2653 transcripts that were significantly altered. We then performed KEGG pathway and module enrichment of the DEGs. Interestingly, most genes involved bacterial chemotaxis-mediated motility, ascorbate and trehalose metabolisms, and sugar transporters including l-arabinose and d-xylose were found to be highly enriched. The in vivo transcriptional profiling of pathogenic B. glumae will facilitate elucidation of unknown plant-pathogenic bacteria interactions, as well as the overall infection processes.
authors.affiliation: Department of Microbiology, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Sunyoung
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Jungwook
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Microbiology, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Jongyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Functional Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang, Gyeongnam, 627-803, Republic of Korea.
authors.forename: Dongjin
authors.initials: D
authors.lastname: Shin
authors.affiliation: Department of Functional Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang, Gyeongnam, 627-803, Republic of Korea.
authors.forename: Dong-Soo
authors.initials: DS
authors.lastname: Park
authors.affiliation: NICEM, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Jong-Sung
authors.initials: JS
authors.lastname: Lim
authors.affiliation: NICEM, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Ik-Young
authors.initials: IY
authors.lastname: Choi
authors.affiliation: Department of Microbiology, Pusan National University, Busan 609-735, Republic of Korea. Electronic address: yseo2011@pusan.ac.kr.
authors.forename: Young-Su
authors.initials: YS
authors.lastname: Seo
mesh_terms: D019117:Burkholderia / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D005798:Genes, Bacterial; D012275:Oryza / Q000235:genetics / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D015398:Signal Transduction; D059467:Transcriptome
keywords: Burkholderia glumae; In vivo transcriptome; Pathogen; RNA-seq; Rice