title: Rational design of biotinylated probes: fluorescent turn-on detection of (strept)avidin and bioimaging in cancer cells.
abstract: Two fluorescent probes SPS1 and SPS2 were designed by connecting biotin to an environment-sensitive coumarin fluorophore. Streptavidin and avidin induced dramatical fluorescence changes in both probes. SPS2 has potential in fluorescent imaging of biotin receptor-enriched tumor cells.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China. junhongqian@ecust.edu.cn weibingzhang@ecust.edu.cn.
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Junhong
authors.initials: J
authors.lastname: Qian
authors.forename: Haiyu
authors.initials: H
authors.lastname: Tian
authors.forename: Liping
authors.initials: L
authors.lastname: Duan
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D003374:Coumarins / Q000138:chemical synthesis* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D018613:Microscopy, Confocal; D015394:Molecular Structure; D017978:Receptors, Growth Factor / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D003374:Coumarins; D017978:Receptors, Growth Factor; C052136:biotin receptor; D001360:Avidin; D001710:Biotin; D019809:Streptavidin