title: [The effect of vascular endothelial cells on the migration of periodontal ligament cells and gingival fibroblasts].
abstract: OBJECTIVE
  To investigate the effect of human vascular endothelial cells (HUVEC) on the migration of human periodontal ligament cells (HPDLC) and human gingival fibroblasts (HGF) through a model mimicking periodontal wound healing or regeneration.
  
  
  METHODS
  HUVEC, HPDLC and HGF were co-cultured using a Transwell system, while HPDLC or HGF was cultured independently as control. Chemotaxis assay was performed using cell culture inserts (0.8μm pore size) to assess the effect of HUVEC on HPDLC and HGF vertical migration at 24h. A scratch wound assay was performed to assess the effect of HUVEC on HPDLC and HGF horizontal migration at 0 h, 8 h, 16 h and 24 h. A round glass sheet assay was performed to assess the effect of HUVEC on HPDLC and HGF wound healing ability at 1d, 4d and 7d. The data was processed with SPSS 13.0 software package.
  
  
  RESULTS
  At the round glass sheet assay, while HUVEC was present, the integrated option density (IOD) of HPDLCs and HGFs were significantly higher than each of the single culture (P<0.01). Results of chemotaxis assay and scratch wound assay showed that the cell number of HPDLCs and HGFs were significantly higher than each of the single culture while HUVEC was present (P<0.01), and the number of PHDLCs was significantly higher than HGFs on chemotaxis assay (P<0.01) but the result of scratch wound assay was on the contrary (P<0.01).
  
  
  CONCLUSIONS
  The presence of HUVEC can promote migration of periodontal cells, and the effect is more prominent on HPDLC than on HGF in vertical migration and wound healing, while horizontal migration is more prominent on HGFs as contrast.
authors.affiliation: Department of Periodontics, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China.E-mail:cqxiaoding2009@126.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Din
authors.forename: Yuan-zheng
authors.initials: YZ
authors.lastname: Yin
authors.forename: Jia-ni
authors.initials: JN
authors.lastname: Huang
authors.forename: Cong-jiao
authors.initials: CJ
authors.lastname: Hu
mesh_terms: D002465:Cell Movement; D002478:Cells, Cultured; D042783:Endothelial Cells; D005347:Fibroblasts; D005881:Gingiva; D006801:Humans; D010513:Periodontal Ligament; D014945:Wound Healing