title: Multilocus sequence analysis of putative Vibrio mediterranei strains and description of Vibrio thalassae sp. nov.
abstract: A multilocus sequence analysis based on partial gyrB, mreB, rpoD and pyrH genes was undertaken with 61 putative Vibrio mediterranei/V. shilonii strains from different hosts (mussels, oysters, clams, coral, fish and plankton) or habitat (seawater and sediment) and geographical origins (Mediterranean, Atlantic and Pacific). A consistent grouping was obtained with individual and concatenated gene sequences, and the clade, comprising 54 strains, was split into three subclades by all methods: subclade A (40 strains, including AK1, the former type strain of Vibrio shilonii), subclade B (8 strains) corresponding to the species V. mediterranei, and subclade C (six strains) representing a new species, V. thalassae sp. nov., with strain MD16(T) (=CECT 8203(T)=KCTC 32373(T)) as the proposed type strain. Average nucleotide identity (ANI) values, determined as a measure of genomic similarity, confirmed these assignments, and supported that strains in subclade C were a different species from V. mediterranei, with ANIb and ANIm figures lower than 90.0%. The synonymy of V. shilonii and V. mediterranei was also stressed by both MLSA and ANI determinations (97.0% between both type strains). No connection was found between geographic origin or sample type and MLSA grouping.
authors.affiliation: Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Tarazona
authors.affiliation: Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Lucena
authors.affiliation: Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Spain.
authors.forename: David R
authors.initials: DR
authors.lastname: Arahal
authors.affiliation: Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Macián
authors.affiliation: Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Spain.
authors.forename: María A
authors.initials: MA
authors.lastname: Ruvira
authors.affiliation: Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Spain. Electronic address: Maria.J.Pujalte@uv.es.
authors.forename: María J
authors.initials: MJ
authors.lastname: Pujalte
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: ANI; MLSA; Partial genome sequences; Vibrio mediterranei; Vibrio shiloi; Vibrio shilonii; Vibrio thalassae