title: Comparison of the adipogenesis in intramuscular and subcutaneous adipocytes from Bamei and Landrace pigs.
abstract: Fat deposition is a complex process involving proliferation, differentiation, and lipogenesis of adipocytes. Bamei and Landrace are considered to represent fat- and lean-type pig breeds. Subcutaneous (SC) and intramuscular (IM) pre-adipocytes were cultured to compare the proliferation and lipogenesis in these breeds. The differentiated adipocytes were exposed to glucose or insulin to evaluate their effects on lipogenesis and lipogenic gene expression. Pre-adipocytes proliferated dramatically faster in SC vs. IM cells, and in Bamei vs. Landrace breeds. Lipogenesis and lipogenic gene expression had a greater increase in Bamei than in Landrace, and in SC vs. IM in the process of differentiation. Glucose markedly promoted lipogenesis and lipogenic gene expression in differentiated adipocytes. The stimulation of high-glucose levels on lipogenesis and ChREBP and lipogenic gene expression was higher in SC than IM adipocytes, and in Bamei vs. Landrace. Insulin largely increased SREBP-1c expression, however it modestly stimulated lipogenesis and lipogenic gene expression, and there was no difference between cell populationsor between breeds. These data demonstrated that regional and varietal differences obviously existed in the development of porcine adipocytes. The proliferation and differentiation capacity of pre-adipocytes, and the adipocyte lipogenesis stimulated by glucose, are stronger in Bamei than Landrace, and in SC vs. IM adipocytes independent of breed.
authors.affiliation: College of Life Science and Engineering, Northwest University for Nationalities, 1 Xibei xin cun, Lanzhou 730030/Gansu Engineering Research Center for Animal Cell, Lanzhou 730030, China.
authors.forename: Guo Hua
authors.initials: GH
authors.lastname: Zhang
authors.forename: Jian Xiong
authors.initials: JX
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Yong Qing
authors.initials: YQ
authors.lastname: Zhao
authors.forename: Peng Hui
authors.initials: PH
authors.lastname: Guo
authors.forename: Ju Tian
authors.initials: JT
authors.lastname: Yang
authors.forename: Rong Xin
authors.initials: RX
authors.lastname: Zang
mesh_terms: D017667:Adipocytes / Q000502:physiology*; D050156:Adipogenesis; D050154:Adiposity; D000818:Animals; D001947:Breeding; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D015870:Gene Expression; D005947:Glucose / Q000502:physiology; D007328:Insulin / Q000502:physiology; D050155:Lipogenesis; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology*; D050151:Subcutaneous Fat / Q000166:cytology / Q000502:physiology*; D034421:Sus scrofa / Q000502:physiology*
chemical_list: D007328:Insulin; D005947:Glucose
keywords: Bamei pigs; adipocytes intramusculaires; adipocytes sous-cutanés; adipogenesis; adipogenèse; intramuscular adipocytes; porcs Bamei; proliferation; prolifération; subcutaneous adipocytes