title: Functional analysis of FABP3 in the milk fat synthesis signaling pathway of dairy cow mammary epithelial cells.
abstract: Milk fat is the major energy component of milk, and regulation of its production relies on transcription factors sterol regulatory element-binding protein 1 (SREBP1) and peroxisome proliferator-activated receptor gamma (PPARγ). As one of the target genes of SREBP1 and PPARγ, fatty acid-binding protein 3(FABP3) is the main protein allowing for rapid diffusion and selective targeting of long-chain fatty acids toward specific organelles for metabolism. Whether FABP3 plays an important role in milk fat synthesis signaling pathway is largely unknown. In this study, we observed the effects of FABP3 overexpression and gene silencing in dairy cow mammary epithelial cells, as well as the effects of oleic acid, stearic acid, and palmitic acid on the expressions of FABP3 and lipid droplet formation, by using quantitative reverse transcriptase (qRT)-PCR, Western blotting, and fluorescent immunostaining techniques. FABP3 upregulated the expression of SREBP1 and PPARγ to increase lipid droplet accumulation. Oleic acid, stearic acid, and palmitic acid also increased lipid droplet accumulation by affecting expression of FABP3. These findings shed new insights for understanding the mechanism of FABP3 in regulating milk fat synthesis.
authors.affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Mucai Street 59, Xiangfang District, Harbin, 150030, Heilongjiang Province, China.
authors.forename: Meng-yao
authors.initials: MY
authors.lastname: Liang
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Hou
authors.forename: Bo
authors.initials: B
authors.lastname: Qu
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Ying-jun
authors.initials: YJ
authors.lastname: Cui
authors.forename: Qing-zhang
authors.initials: QZ
authors.lastname: Li
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Gao
mesh_terms: D000818:Animals; D002417:Cattle; D004847:Epithelial Cells / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D020868:Gene Silencing; D066292:Lipid Droplets / Q000378:metabolism; D050356:Lipid Metabolism; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D050556:Fatty Acid-Binding Proteins