title: Using a five-gene phylogeny to test morphology-based hypotheses of Smittium and allies, endosymbiotic gut fungi (Harpellales) associated with arthropods.
abstract: Smittium, one of the first described genera of gut fungi, is part of a larger group of endosymbiotic microorganisms (Harpellales) that live predominantly in the digestive tracts of aquatic insects. As a diverse and species-rich taxon, Smittium has helped to advance our understanding of the gut fungi, in part due to the relative success of attempts to culture species of Smittium as compared to other members of Harpellales. Approximately 40% of the 81 known species of Smittium have been cultured. This is the first Smittium multigene dataset and phylogenetic analysis, using the 18S and 28S rRNA genes, as well as RPB1, RPB2, and MCM7 translated protein sequences. Several well-supported clades were recovered within Smittium. One includes the epitype S. mucronatum (the "True Smittium" clade), and another contains many species including S. simulii and S. orthocladii (the "Parasmittium" clade). Ancestral states were reconstructed for holdfast shape, thallus branching type, as well as asexual (trichospore) and sexual (zygospore) spore morphology. Two of these characters, holdfast shape and trichospore morphology, supported the split of the two main clades revealed by the molecular phylogeny, suggesting these are natural clades and these traits may have evolutionary and perhaps ecological significance.
authors.affiliation: Boise State University, Department of Biological Sciences, Boise, ID 83725-1515, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Boise State University, Department of Biological Sciences, Boise, ID 83725-1515, USA.
authors.forename: Eric D
authors.initials: ED
authors.lastname: Tretter
authors.affiliation: Boise State University, Department of Biological Sciences, Boise, ID 83725-1515, USA.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Johnson
authors.affiliation: Boise State University, Department of Biological Sciences, Boise, ID 83725-1515, USA.
authors.forename: Prasanna
authors.initials: P
authors.lastname: Kandel
authors.affiliation: University of Kansas, Department of Ecology & Evolutionary Biology, Lawrence, KS 66045-7534, USA.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Lichtwardt
authors.affiliation: Boise State University, Department of Biological Sciences, Boise, ID 83725-1515, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Novak
authors.affiliation: Boise State University, Department of Biological Sciences, Boise, ID 83725-1515, USA.
authors.forename: James F
authors.initials: JF
authors.lastname: Smith
authors.affiliation: Boise State University, Department of Biological Sciences, Boise, ID 83725-1515, USA. Electronic address: merlinwhite@boisestate.edu.
authors.forename: Merlin M
authors.initials: MM
authors.lastname: White
mesh_terms: D000818:Animals; D004271:DNA, Fungal / Q000235:genetics; D004064:Digestive System / Q000382:microbiology; D005658:Fungi / Q000145:classification* / Q000235:genetics; D005800:Genes, Fungal; D007313:Insecta / Q000382:microbiology*; D008957:Models, Genetic; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004271:DNA, Fungal; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S
keywords: Aquatic insects; Evolution; Fungi; Kickxellomycotina; Symbiosis; Trichomycetes