title: Live pups from evaporatively dried mouse sperm stored at ambient temperature for up to 2 years.
abstract: The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, a single sperm was injected into a mature oocyte to develop into a blastocyst after culture or a live birth after embryo transfer to a recipient female. For the samples stored at 4°C for 3, 6, 12, 18, and 24 months, the blastocyst formation rate was 61.5%, 49.1%, 31.5%, 32.2%, and 41.4%, respectively. The blastocyst rate for those stored at ambient temperature (∼22°C) for 3, 6, 12, and 18 months was 57.8%, 36.2%, 33.6%, and 34.4%, respectively. Fifteen, eight and three live pups were produced from sperm stored at room temperature for 12, 18, and 24 months, respectively. This is the first report of live offspring produced from dried mouse sperm stored at ambient temperature for up to 2 years. Based on these results, we suggest that evaporative drying is a potentially useful method for the routine preservation of mouse sperm.
authors.affiliation: Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, Massachusetts, United States of America.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, Massachusetts, United States of America.
authors.forename: Gloria Y
authors.initials: GY
authors.lastname: Lee
authors.affiliation: Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America.
authors.forename: Joel A
authors.initials: JA
authors.lastname: Lawitts
authors.affiliation: Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, Massachusetts, United States of America.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Toner
authors.affiliation: Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, Massachusetts, United States of America; Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, United States of America.
authors.forename: John D
authors.initials: JD
authors.lastname: Biggers
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003890:Desiccation; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D050498:Live Birth / Q000662:veterinary; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D011247:Pregnancy; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary; D020554:Sperm Injections, Intracytoplasmic / Q000662:veterinary; D013696:Temperature; D013997:Time Factors