title: Filamentous fungi and media for cellulase production in solid state cultures.
abstract: Cellulase production was evaluated in two reference strains (T. reesei Rut-C30 and T. reesei QM9414), two strains isolated from a sugarcane cultivation area (Trichoderma sp. IPT778 and T. harzianum rifai IPT821) and one strain isolated in a program for biodiversity preservation in São Paulo state (Myceliophthora thermophila M77). Solid state cultures were performed using sugarcane bagasse (C), wheat bran (W) and/or soybean bran (S). The highest FPA was 10.6 U/gdm for M77 in SC (10:90) at 80% moisture, which was 4.4 times higher than production in pure W. C was a strong inducer of cellulase production, given that the production level of 6.1 U/gdm in WC (40:60) was 2.5 times higher than in pure W for strain M77; T. reesei Rut-C30 did not respond as strongly with about 1.6-fold surplus production. S advantageously replaced W, as the surplus production on SC (20:80) was 2.3 times relative to WC (20:80) for M77.
authors.affiliation: Departamento de Engenharia Química Universidade de São Paulo São PauloSP Brazil.
authors.forename: B V
authors.initials: BV
authors.lastname: Kilikian
authors.affiliation: Departamento de Engenharia Química Universidade de São Paulo São PauloSP Brazil.
authors.forename: L C
authors.initials: LC
authors.lastname: Afonso
authors.affiliation: Departamento de Engenharia Química Universidade de São Paulo São PauloSP Brazil.
authors.forename: T F C
authors.initials: TF
authors.lastname: Souza
authors.affiliation: Departamento de Engenharia Química Universidade de São Paulo São PauloSP Brazil.
authors.forename: R G
authors.initials: RG
authors.lastname: Ferreira
authors.affiliation: Departamento de Engenharia Rural Centro de Ciências Agrárias Universidade Federal do Espírito Santo GuararemaES Brazil.
authors.forename: I R
authors.initials: IR
authors.lastname: Pinheiro
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D004043:Dietary Fiber / Q000378:metabolism; D005658:Fungi / Q000201:enzymology* / Q000254:growth & development*; D031786:Saccharum / Q000378:metabolism; D020037:Sordariales / Q000201:enzymology / Q000254:growth & development; D013025:Glycine max / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology / Q000254:growth & development
chemical_list: D003470:Culture Media; D004043:Dietary Fiber; D002480:Cellulase
keywords: Myceliophtora sp; cellulase; productivity; solid media; sugarcane bagasse