title: Direct patterning of poly(acrylic acid) on polymer surfaces by ion beam lithography for the controlled adhesion of mammalian cells.
abstract: Poly(acrylic acid) (PAA)-patterned polystyrene (PS) substrates were prepared by ion beam lithography to control cell behaviors of mouse fibroblasts and human embryonic kidney cells. Thin PAA films spin-coated on non-biological PS substrates were selectively irradiated with energetic proton ions through a pattern mask. The irradiated substrates were developed with deionized water to generate negative-type PAA patterns. The surface characteristics of the resulting PAA-patterned PS surface, such as surface morphology, chemical structure and composition and wettability, were investigated. Well-defined 100 μm PAA patterns were effectively formed on relatively hydrophobic PS substrates by ion beam lithography at higher fluences than 5 × 10(14) ions/cm(2). Moreover, based on the in vitro cell culture test, cells were adhered and proliferated favorably onto hydrophilic PAA regions separated by hydrophobic PS regions on the PAA-patterned PS substrates, and thereby leading to the formation of well-defined cell patterns.
authors.affiliation: Research Division for Industry and Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeollabuk-do, Jeongeup-si, 580-185, Republic of Korea.
authors.forename: In-Tae
authors.initials: IT
authors.lastname: Hwang
authors.forename: Min-Suk
authors.initials: MS
authors.lastname: Oh
authors.forename: Chan-Hee
authors.initials: CH
authors.lastname: Jung
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Choi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry* / Q000528:radiation effects*; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000528:radiation effects*; D057809:HEK293 Cells; D006801:Humans; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D023822:Tissue Engineering / Q000379:methods; D014466:Ultraviolet Rays; D017155:Wettability
chemical_list: D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; C006903:carbopol 940