title: Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle.
abstract: The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered important for the serological diagnosis of A. marginale, as they indicate that the test represents a rapid and low cost alternative to ELISA.
authors.affiliation: Embrapa Gado de Corte Campo GrandeMS Brazil.
authors.forename: Carlos A N
authors.initials: CA
authors.lastname: Ramos
authors.affiliation: Embrapa Gado de Corte Campo GrandeMS Brazil.
authors.forename: Flábio R
authors.initials: FR
authors.lastname: Araújo
authors.affiliation: Programa de Pós Graduação em Saúde Animal na Amazônia Universidade Federal do Pará CastanhalPA Brazil.
authors.forename: Rafaelle C
authors.initials: RC
authors.lastname: Santos
authors.affiliation: Programa de Pós Graduação em Ciência Animal Universidade Federal de Mato Grosso do Sul Campo GrandeMS Brazil.
authors.forename: Elaine S P
authors.initials: ES
authors.lastname: Melo
authors.affiliation: Programa de Pós Graduação em Ciência Animal Universidade Federal de Mato Grosso do Sul Campo GrandeMS Brazil.
authors.forename: Letícia C
authors.initials: LC
authors.lastname: Sousa
authors.affiliation: Programa de Pós Graduação em Medicina Veterinária Universidade Federal de Santa Maria Santa MariaRS Brazil.
authors.forename: Carlos E S
authors.initials: CE
authors.lastname: Vidal
authors.affiliation: Programa de Pós Graduação em Ciência Animal Tropical Universidade Federal Rural de Pernambuco RecifePE Brazil.
authors.forename: Neurisvan R
authors.initials: NR
authors.lastname: Guerra
authors.affiliation: Department of Veterinary Medicine Universita degli Studi de Bari ValenzanoBari Italy.
authors.forename: Rafael A N
authors.initials: RA
authors.lastname: Ramos
mesh_terms: D042322:Anaplasma marginale / Q000235:genetics / Q000276:immunology*; D000712:Anaplasmosis / Q000175:diagnosis*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D007841:Latex Fixation Tests / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014730:Veterinary Medicine / Q000379:methods
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins; C111578:major surface protein 5, Anaplasma
keywords: Anaplasma marginale; MSP5; latex agglutination; rapid test