title: Cord blood CD4(+)CD25(+) regulatory T cells fail to inhibit cord blood NK cell functions due to insufficient production and expression of TGF-beta1.
abstract: Although CD4(+)CD25(+) Treg (Treg) cells are known to modulate NK cell functions, the modulation mechanism of these cells in cord blood has not been fully clarified. The purpose of this study was to clarify the mechanism whereby cord blood Treg cells modulate cord NK cells. By performing various cultures of purified NK cells with or without autologous Treg cells, diminished inhibitory effects of cord Treg cells towards cord NK cell functions, including activation, cytokine production, and cytotoxicity, were observed. We also observed lower secretion of sTGF-beta1 and lower expression of mTGF-beta1 by cord Treg cells than by adult Treg cells. These data revealed the capability of adult Treg cells to suppress rhIL-2-stimulated NK cell function by TGF-beta1, both membrane-bound and soluble types. The reduced inhibitory capabilities of cord Treg cells compared with adult Treg cells is thought to be due to insufficient expression of TGF-beta1.
authors.affiliation: Clinical Research Institute, Mie Chuo Medical Center, National Hospital Organization, Tsu, Mie, Japan; Department of Neonatal Science, Institute of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu, Mie, Japan; Shanghai Children's Medical Center, Shanghai, China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Xu
authors.affiliation: Clinical Research Institute, Mie Chuo Medical Center, National Hospital Organization, Tsu, Mie, Japan; Department of Neonatal Science, Institute of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu, Mie, Japan. Electronic address: tanakash@miechuo-m.hosp.go.jp.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: Clinical Research Institute, Mie Chuo Medical Center, National Hospital Organization, Tsu, Mie, Japan; Department of Neonatal Science, Institute of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
authors.forename: Motoki
authors.initials: M
authors.lastname: Bonno
authors.affiliation: Clinical Research Institute, Mie Chuo Medical Center, National Hospital Organization, Tsu, Mie, Japan; Department of Neonatal Science, Institute of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ido
authors.affiliation: Department of Psychology and Social Welfare, School of Letters, Mukogawa Women's University, Nishinomiya, Hyogo, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kawai
authors.affiliation: Clinical Research Institute, Mie Chuo Medical Center, National Hospital Organization, Tsu, Mie, Japan; Department of Neonatal Science, Institute of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
authors.forename: Hatsumi
authors.initials: H
authors.lastname: Yamamoto
authors.affiliation: Department of Pediatrics, Institute of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Komada
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D015704:CD4 Antigens / Q000276:immunology; D019002:CD56 Antigen / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D007376:Interleukin-2 / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*; D055815:Young Adult; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D019002:CD56 Antigen; D016207:Cytokines; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D053773:Transforming Growth Factor beta1; D000099192:CD69 Antigens; C508594:IL2 protein, human; C508017:IL2RA protein, human
keywords: CD4(+)CD25(+) T regulatory cells; NK cells; TGF-beta1; Umbilical cord blood