title: Resveratrol modulates apoptosis in 5-fluorouracyl treated colon cancer cell lines.
abstract: Since cancer is a cellular disease, it is essential to identify the development stages and use the information in the prediction, prevention, early detection and design of drug targets. Colon cancer represents a malignancy with high incidence and mortality throughout the world, its etiology involving many genetic, immunological and biochemical factors. 5-fluorouracyl (5-FU) is one of the most effective anti-cancer agents used in the treatment of colorectal cancers, but tumor chemoresistance is a major limiting factor of its use. In order to choose the most effective chemotherapeutic doses of 5-FU, and thereby diminish the side-effects, we tried to modulate the anticancer properties of 5-FU by adding dietary natural compounds. The study focused on the role of natural compounds as resveratrol (RSV) in sensitization of LoVo human colon adenocarcinoma cell line to 5-FU action. Real-time cell analysis (RTCA) by xCELLigence System was used to continuously monitor the cytotoxic effects of drug treatments on LoVo cells. RTCA allowed us to choose the proper concentrations for further end-point assays, such as flow-cytometry techniques used for the evaluation of apoptotic events, progression through cell cycle phases or nuclear antigen expression of compound-treated LoVo cells. Data obtained showed additional effects of RSV to 5-FU treatments on the increase ofapoptotic events, and suggested alternative approaches to obtain a stronger antitumor response, and diminished side-effects when low concentrations of anti-cancer drugs are used. Modulation of the mechanisms of programmed cell death process seem to be of great importance for malignant transformation, and therefore for anti-cancer therapeutic approaches.
authors.forename: Dan
authors.initials: D
authors.lastname: Hotnog
authors.forename: Mirela
authors.initials: M
authors.lastname: Mihăilă
authors.forename: Iulia Virginia
authors.initials: IV
authors.lastname: Lancu
authors.forename: Georgiana Gabriela
authors.initials: GG
authors.lastname: Matei
authors.forename: Camelia
authors.initials: C
authors.lastname: Hotnog
authors.forename: Gabriela
authors.initials: G
authors.lastname: Anton
authors.forename: Marinela
authors.initials: M
authors.lastname: Bostan
authors.forename: Lorelei I
authors.initials: LI
authors.lastname: Braşoveanu
mesh_terms: D000970:Antineoplastic Agents; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000503:physiopathology; D005472:Fluorouracil / Q000009:adverse effects*; D006801:Humans; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D013267:Stilbenes; D000077185:Resveratrol; D005472:Fluorouracil