title: Cytotoxic effects of dillapiole on embryonic development of mouse blastocysts in vitro and in vivo.
abstract: We examined the cytotoxic effects of dillapiole, a phenylpropanoid with antileishmanial, anti-inflammatory, antifungal, and acaricidal activities, on the blastocyst stage of mouse embryos, subsequent embryonic attachment and outgrowth in vitro, and in vivo implantation via embryo transfer. Blastocysts treated with 2.5-10 μM dillapiole exhibited a significant increase in apoptosis and corresponding decrease in total cell number. Notably, the implantation success rates of blastocysts pretreated with dillapiole were lower than those of their control counterparts. Moreover, in vitro treatment with 2.5-10 μM dillapiole was associated with increased resorption of post-implantation embryos and decreased fetal weight. Our results collectively indicate that dillapiole induces apoptosis and retards early post-implantation development, both in vitro and in vivo. However, the extent to which this organic compound exerts teratogenic effects on early human development is not known at present. Further studies are required to establish effective protection strategies against the cytotoxic effects of dillapiole.
authors.affiliation: Department of Bioscience Technology and Center for Nanotechnology, Chung Yuan Christian University, Chung Li 32023, Taiwan. whchan@cycu.edu.tw.
authors.forename: Wen-Hsiung
authors.initials: WH
authors.lastname: Chan
mesh_terms: D000498:Allyl Compounds / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004149:Dioxoles / Q000633:toxicity*; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR
chemical_list: D000498:Allyl Compounds; D004149:Dioxoles; C498255:5-allyl 6,7-dimethoxy 1,3-benzodioxole