title: Phenotypic and genotypic characterization of some lactic Acid bacteria isolated from bee pollen: a preliminary study.
abstract: In the present work, five hundred and sixty-seven isolates of lactic acid bacteria were recovered from raw bee pollen grains. All isolates were screened for their antagonistic activity against both Gram-positive and Gram-negative pathogenic bacteria. Neutralized supernatants of 54 lactic acid bacteria (LAB) cultures from 216 active isolates inhibited the growth of indicator bacteria. They were phenotypically characterized, based on the fermentation of 39 carbohydrates. Using the simple matching coefficient and unweighted pair group algorithm with arithmetic averages (UPGMA), seven clusters with other two members were defined at the 79% similarity level. The following species were characterized: Lactobacillus plantarum, Lactobacillus fermentum, Lactococcus lactis, Pediococcus acidilactici, Pediococcus pentosaceus, and unidentified lactobacilli. Phenotypic characteristics of major and minor clusters were also identified. Partial sequencing of the 16S rRNA gene of representative isolates from each cluster was performed, and ten strains were assigned to seven species: Lactobacillus plantarum, Lactobacillus fermentum, Lactococcus lactis, Lactobacillus ingluviei, Pediococcus pentosaceus, Lactobacillus acidipiscis and Weissella cibaria. The molecular method used failed to determine the exact taxonomic status of BH0900 and AH3133.
authors.affiliation: Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Sétif 1 Ferhat Abbas, 19000 Sétif, Algeria.
authors.forename: Hani
authors.initials: H
authors.lastname: Belhadj
authors.affiliation: Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Sétif 1 Ferhat Abbas, 19000 Sétif, Algeria.
authors.forename: Daoud
authors.initials: D
authors.lastname: Harzallah
authors.affiliation: Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natural and life Sciences, University Sétif 1 Ferhat Abbas, 19000 Sétif, Algeria.
authors.forename: Dalila
authors.initials: D
authors.lastname: Bouamra
authors.affiliation: Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natural and life Sciences, University Sétif 1 Ferhat Abbas, 19000 Sétif, Algeria.
authors.forename: Seddik
authors.initials: S
authors.lastname: Khennouf
authors.affiliation: Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natural and life Sciences, University Sétif 1 Ferhat Abbas, 19000 Sétif, Algeria.
authors.forename: Saliha
authors.initials: S
authors.lastname: Dahamna
authors.affiliation: Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Sétif 1 Ferhat Abbas, 19000 Sétif, Algeria ; Laboratory of Plant Biotechnology, Department of Natural and Life Sciences, Sciences Faculty, University of M'sila, PO Box 166 M'sila, Algeria.
authors.forename: Mouloud
authors.initials: M
authors.lastname: Ghadbane
keywords: 16S rRNA gene sequencing; UPGMA; functional foods; lactic acid bacteria; numerical clustering; pollen