title: Plant DNA-damage repair/toleration 100 protein repairs UV-B-induced DNA damage.
abstract: We report the characterization of VvDRT100-L, a grape DNA-damage repair/toleration 100 protein. VvDRT100-L has nine leucine-rich repeats and belongs to the plant DRT100 protein family. VvDRT100-L is expressed abundantly in green organs of grapevines, including tendrils, leaves, and green berry skins. The overexpression of VvDRT100-L in Arabidopsis plants decreased the number of abasic sites and the frequency of DNA single-strand breaks in the DNA damaged by UV-B irradiation, whereas UV-B irradiation markedly increased the number of abasic sites and the frequency of DNA single-strand breaks in T-DNA insertion mutant drt100 plants. VvDRT100-L-overexpressing plants remained viable and noticeably healthy under lethal UV doses, suggesting that VvDRT100-L may enhance UV tolerance in plant. Taken together, we concluded that VvDRT100-L might play an important role in the repair and toleration of UV-B-induced DNA damage. These findings would help us better understand how plants acquire UV stress acclimation, tolerance and DNA repair.
authors.affiliation: Laboratory of Fruit Genetics Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Fujimori
authors.affiliation: Laboratory of Fruit Genetics Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan.
authors.forename: Nana
authors.initials: N
authors.lastname: Suzuki
authors.affiliation: Laboratory of Fruit Genetics Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakajima
authors.affiliation: Laboratory of Fruit Genetics Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan. Electronic address: suzukis@yamanashi.ac.jp.
authors.forename: Shunji
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D053904:DNA Breaks, Single-Stranded; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011836:Radiation Tolerance; D014466:Ultraviolet Rays; D027843:Vitis / Q000201:enzymology / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D045643:DNA Repair Enzymes
keywords: Abasic sites; DNA damage; DNA single-strand breaks; DNA-damage repair/toleration 100; Grape; Leucine-rich repeat; UV-B