title: Detection of hepatitis C virus coinfection in patients with dengue diagnosis.
abstract: Coinfection produced by dengue virus (DENV) and hepatitis C virus (HCV) is a serious problem of public health in Mexico, as they both circulate in tropical zones and may lead to masking or complicating symptoms. In this research, we detected active coinfected patients by HCV residing in the endemic city of Mérida, Yucatán, Mexico, with positive diagnosis to dengue during the acute phase. We performed a retrospective analysis of 240 serum samples from dengue patients. The IgM-ELISA serological test was used for dengue diagnosis, as well as viral isolation to confirm infection. DENV and HCV were detected by RT-PCR. Thus, 31 (12.9%) samples showed DENV-HCV coinfection, but interestingly the highest frequency of coinfection cases was found in male patients presenting hemorrhagic dengue in 19/31 (61.29%), with a predominance of 12 : 7 in males. Firstly, coinfection of DENV-HCV in Mérida, Mexico, was detected in young dengue patients, between 11 and 20 years old (38.7%), followed by those between 21 and 30 years old (32%); only 16.13% were between 0 and 10 years of age. Diagnosis of HCV infection in patients with dengue is highly recommended in order to establish potential risk in clinical manifestations as well as dictate patients' special care.
authors.affiliation: Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi (Unidad Inalámbrica), Universidad Autónoma de Yucatán, 97225 Mérida, YUC, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Machain-Williams
authors.affiliation: Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi (Unidad Inalámbrica), Universidad Autónoma de Yucatán, 97225 Mérida, YUC, Mexico.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Talavera-Aguilar
authors.affiliation: Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi (Unidad Inalámbrica), Universidad Autónoma de Yucatán, 97225 Mérida, YUC, Mexico.
authors.forename: Rosa Carmina
authors.initials: RC
authors.lastname: Cetina-Trejo
authors.affiliation: Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales Dr. Hideyo Noguchi (Unidad Itzáes), Universidad Autónoma de Yucatán, 97000 Mérida, YUC, Mexico.
authors.forename: Jaquelin
authors.initials: J
authors.lastname: Carrillo-Navarrete
authors.affiliation: Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi (Unidad Inalámbrica), Universidad Autónoma de Yucatán, 97225 Mérida, YUC, Mexico.
authors.forename: Nubia
authors.initials: N
authors.lastname: Rivero-Cárdenas
authors.affiliation: Laboratorio de Inmunología Celular e Inmunopatogénesis, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 Mexico, DF, Mexico.
authors.forename: Ma Isabel
authors.initials: MI
authors.lastname: Salazar
authors.affiliation: Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi (Unidad Inalámbrica), Universidad Autónoma de Yucatán, 97225 Mérida, YUC, Mexico.
authors.forename: José Arturo
authors.initials: JA
authors.lastname: Farfán-Ale
authors.affiliation: Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales Dr. Hideyo Noguchi (Unidad Itzáes), Universidad Autónoma de Yucatán, 97000 Mérida, YUC, Mexico.
authors.forename: Fernando I
authors.identifier: 0000-0001-9726-2039
authors.initials: FI
authors.lastname: Puerto
authors.affiliation: Laboratorio de Inmunología Celular e Inmunopatogénesis, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 Mexico, DF, Mexico.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Castro-Mussot
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D060085:Coinfection / Q000235:genetics / Q000821:virology; D003715:Dengue / Q000175:diagnosis* / Q000235:genetics / Q000821:virology*; D003716:Dengue Virus / Q000302:isolation & purification* / Q000472:pathogenicity; D005260:Female; D016174:Hepacivirus / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged