title: Protective efficacies and immune responses induced by recombinant HCD, atpD and gdhA against bacterial cold-water disease in ayu (Plecoglossus altivelis).
abstract: Protective efficacies of three antigenic proteins (3-hydroxyacyl-CoA dehydrogenase (HCD), ATP synthase beta subunit (atpD), and glutamate dehydrogenase (gdhA)) against Flavobacterium psychrophilum were investigated in ayu (Plecoglossus altivelis). Recombinant proteins of HCD, atpD, and gdhA were expressed in Escherichia coli BL21 cells. Ayu were then vaccinated with inactivated cells via the intraperitoneal route. Compared with the empty BL21- and PBS-injected groups, the vaccinated group had a significantly longer survival time after challenge with F. psychrophilum. The antibody titers against each recombinant protein were significantly higher in serum from vaccinated fish, compared with serum from control fish. Results of indirect immunofluorescence assays using serum indicated that the HCD, atpD, and gdhA proteins are located on the surface of F. psychrophilum. These results suggest that these three surface proteins are protective antigens and are good candidates for development of vaccines against bacterial cold-water disease in ayu.
authors.affiliation: Tamaki Laboratory, National Research Institute of Aquaculture, Fisheries Research Agency, 224-1 Hiruta, Tamaki, Watarai, Mie 519-0423, Japan; The Japan Society for the Promotion of Science, 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan.
authors.forename: Goshi
authors.initials: G
authors.lastname: Kato
authors.affiliation: Tamaki Laboratory, National Research Institute of Aquaculture, Fisheries Research Agency, 224-1 Hiruta, Tamaki, Watarai, Mie 519-0423, Japan.
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Sakai
authors.affiliation: Gunma Prefectural Fisheries Experimental Station, 13 Shikishima, Maebashi, Gunma 371-0036, Japan.
authors.forename: Kyuma
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Tamaki Laboratory, National Research Institute of Aquaculture, Fisheries Research Agency, 224-1 Hiruta, Tamaki, Watarai, Mie 519-0423, Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Sano
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, 422-1 Nakatsuhamaura, Minami-ise, Mie 516-0193, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Takano
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, 422-1 Nakatsuhamaura, Minami-ise, Mie 516-0193, Japan. Electronic address: matsuym@fra.affrc.go.jp.
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Matsuyama
authors.affiliation: Tamaki Laboratory, National Research Institute of Aquaculture, Fisheries Research Agency, 224-1 Hiruta, Tamaki, Watarai, Mie 519-0423, Japan.
authors.forename: Chihaya
authors.initials: C
authors.lastname: Nakayasu
mesh_terms: D063988:3-Hydroxyacyl-CoA Dehydrogenase; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000494:pharmacology*; D015153:Blotting, Western / Q000662:veterinary; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology* / Q000517:prevention & control*; D045826:Flavobacteriaceae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D005417:Flavobacterium / Q000235:genetics / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D036441:Osmeriformes
chemical_list: D001428:Bacterial Vaccines; D017931:DNA Primers; D063988:3-Hydroxyacyl-CoA Dehydrogenase; D005969:Glutamate Dehydrogenase; D025261:Mitochondrial Proton-Translocating ATPases
keywords: Ayu Plecoglossus altivelis; Bacterial cold-water disease; Enzyme-linked immunosorbent assay; His-tag fusion protein; Humoral immune response