title: Wild celery (Smyrnium olusatrum L.) oil and isofuranodiene induce apoptosis in human colon carcinoma cells.
abstract: Smyrnium olusatrum (Apiaceae), well known as wild celery, is a biennal celery-scented plant used for many centuries as a vegetable, then abandoned after the introduction of celery. In the present work, the essential oil obtained from inflorescences and the amounts of its main constituents isofuranodiene, curzerene and germacrone were analyzed by GC as well as by HPLC because of their degradation (Cope rearrangement) occurring at high temperatures. The oil and the main constituents were assayed for cytotoxic activity on the human colon cancer cell line (HCT116) by MTT assay. Flower oil and isofuranodiene showed noteworthy activity on tumor cells with IC50 of 10.71 and 15.06 μg/ml, respectively. Analysis of the cytotoxic activity showed that wild celery oil and isofuranodiene are able to induce apoptosis in colon cancer cells in a time and concentration-dependent manner suggesting a potential role as models for the development of chemopreventive agents.
authors.affiliation: School of Pharmacy, University of Camerino, Italy.
authors.forename: Luana
authors.initials: L
authors.lastname: Quassinti
authors.affiliation: School of Pharmacy, University of Camerino, Italy. Electronic address: filippo.maggi@unicam.it.
authors.forename: Filippo
authors.initials: F
authors.lastname: Maggi
authors.affiliation: School of Science and Technology, University of Camerino, Italy.
authors.forename: Luciano
authors.initials: L
authors.lastname: Barboni
authors.affiliation: HPLC-MS Laboratory, University of Camerino, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Ricciutelli
authors.affiliation: HPLC-MS Laboratory, University of Camerino, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Cortese
authors.affiliation: School of Pharmacy, University of Camerino, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Papa
authors.affiliation: School of Bioscience and Veterinary Medicine, University of Camerino, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Garulli
authors.affiliation: School of Bioscience and Veterinary Medicine, University of Camerino, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Kalogris
authors.affiliation: School of Pharmacy, University of Camerino, Italy.
authors.forename: Sauro
authors.initials: S
authors.lastname: Vittori
authors.affiliation: School of Pharmacy, University of Camerino, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Bramucci
mesh_terms: D019661:Apiaceae / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D002277:Carcinoma / Q000188:drug therapy*; D003110:Colonic Neoplasms / Q000188:drug therapy*; D053938:DNA Fragmentation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005663:Furans / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D045325:HCT116 Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D009822:Oils, Volatile / Q000737:chemistry / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D005663:Furans; D009822:Oils, Volatile; D010936:Plant Extracts; C017897:isofuranodiene
keywords: Apoptosis; Colon carcinoma cells; Curzerene pubchem CID 5316217; Essential oil; Germacrone pubchem CID 6436348; Isofuranodiene; Isofuranodiene pubchem CID 5317424; Smyrnium olusatrum