title: Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity.
abstract: A highly potent secondary metabolite producing actinomycetes strain is isolated from marine soil sediments of Visakhapatnam sea coast, Bay of Bengal. Over all ten strains are isolated from the collected soil sediments. Among the ten actinomycetes strains the broad spectrum strain RSPSN2 was selected for molecular characterization, antibiotic production and its purification. The nucleotide sequence of the 1 rRNA gene (1261 base pairs) of the most potent strain evidenced a 96% similarity with Streptomyces parvulus 1044 strain, Streptomyces parvulus NBRC 13193 and Streptomyces parvulus BY-F. From the taxonomic features, the actinomycetes isolate RSPSN2 matches with Streptomyces parvulus in the morphological, physiological and biochemical characters. Thus, it was given the suggested name Streptomyces parvulus RSPSN2. The active metabolite was extracted using ethyl acetate (1:3, v/v) at pH 7.0. The separation of active ingredient and its purification was performed by using both thin layer chromatography (TLC) and column chromatography (CC) techniques. Spectrometric studies such as UV-visible, FTIR, and NMR and mass were performed. The antibacterial activity of pure compound was performed by cup plate method against some pathogenic bacteria including of streptomycin resistant bacteria like (Pseudomonas mirabilis, Pseudomonas putida and Bacillus cereus). In conclusion, the collected data emphasized the fact that a polypeptide antibiotic (Actinomycin D) was produced by Streptomyces parvulus RSPSN2.
authors.affiliation: Bioengineering and Environmental Centre Indian Institute of Chemical Technology Hyderabad India.
authors.forename: Prakasham Reddy
authors.initials: PR
authors.lastname: Shetty
authors.affiliation: Bioengineering and Environmental Centre Indian Institute of Chemical Technology Hyderabad India.
authors.forename: Sudheer Kumar
authors.initials: SK
authors.lastname: Buddana
authors.affiliation: Bioengineering and Environmental Centre Indian Institute of Chemical Technology Hyderabad India.
authors.forename: Vinay Bharadwaj
authors.initials: VB
authors.lastname: Tatipamula
authors.affiliation: Bioengineering and Environmental Centre Indian Institute of Chemical Technology Hyderabad India.
authors.forename: Yaswanth Varanasi Venkata
authors.initials: YV
authors.lastname: Naga
authors.affiliation: Bioengineering and Environmental Centre Indian Institute of Chemical Technology Hyderabad India.
authors.forename: Jamal
authors.initials: J
authors.lastname: Ahmad
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D007194:India; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013302:Streptomyces / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D010455:Peptides; D012335:RNA, Ribosomal
keywords: 1 rRNA characterization; Streptomyces parvulus; Streptomycin resistance; antibacterial activity; polypeptide antibiotic