title: Functional characterization of 5-enopyruvylshikimate-3-phosphate synthase from Alkaliphilus metalliredigens in transgenic Arabidopsis.
abstract: Although a large number of AroA enzymes (EPSPS: 5-enopyruvylshikimate-3-phosphate synthase) have been identified, cloned, and tested for glyphosate resistance, only two AroA variants, derived from Agrobacterium tumefaciens strain CP4 and Zea mays, have been utilized to produce the commercial glyphosate-resistant crops. Here, we have used a PCR-based twostep DNA synthesis method to synthesize an aroA gene (aroAA. metalliredigens) from Alkaliphilus metalliredigens, encoding a new EPSPS. Furthermore, transgenic Arabidopsis with the new aroAA. metalliredigens gene was obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu 030801, P. R. China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Xing
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Tian
authors.forename: Ri-He
authors.initials: RH
authors.lastname: Peng
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Quan-Hong
authors.initials: QH
authors.lastname: Yao
authors.forename: Sheng
authors.initials: S
authors.lastname: Sun
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D004351:Drug Resistance; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000201:enzymology* / Q000235:genetics; D006540:Herbicides / Q000378:metabolism; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000097797:Glyphosate
chemical_list: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D005998:Glycine; D006540:Herbicides; D011994:Recombinant Proteins; D000097797:Glyphosate