title: [Study of genetic polymorphisms of 12 short tandem repeats on X chromosome in ethnic Han population from Hebei Province].
abstract: OBJECTIVE
  To investigate genetic polymorphisms of 12 X chromosome short tandem repeat (X-STR) loci in ethnic Hebei Han population using an Investigator Argus X-12 amplification kit.
  
  
  METHODS
  DNA was extracted for 198 unrelated individuals (96 males and 102 females) and amplified with a fluorescence labeled multiplex PCR system. PCR products were separated and genotyped with capillary array electrophoresis.
  
  
  RESULTS
  Only DXS10103 and DXS10101 showed significant linkage disequilibrium at the 12 X-STR loci. One hundred and forty-eight alleles, including 22 off-ladder (OL) alleles, were observed at the 12 X-STR loci in the population. The heterozygosity and polymorphic information content (PIC) were 0.5074-0.9143 and 0.4377-0.9079, respectively. The power of discrimination (PD) was 0.5074-0.9143 in males and 0.6876-0.9863 in females. The mean exclusion chance was 0.4377-0.9079 in the trios cases and 0.2984-0.8373 in the duo cases, respectively.
  
  
  CONCLUSIONS
  The Investigator Argus X12 amplification system is highly polymorphic in ethnic Han population from Hebei and is useful for personal identification and paternity testing.
authors.affiliation: Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China. Email: shujinli@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Liang
authors.initials: L
authors.lastname: Lei
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.forename: Guangping
authors.initials: G
authors.lastname: Fu
authors.forename: Zhilu
authors.initials: Z
authors.lastname: Xu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Shujin
authors.initials: S
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Cong
mesh_terms: D000483:Alleles; D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D002681:China; D041321:Chromosomes, Human, X / Q000235:genetics*; D005260:Female; D005828:Genetics, Population; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic