title: Expression of AKR1C3 and CNN3 as markers for detection of lymph node metastases in colorectal cancer.
abstract: The aim of the study was to identify a set of discriminating genes that could be used for the prediction of Lymph node (LN) metastasis in human colorectal cancer (CRC), and for this, we compared the whole genome profiles of two CRC cell lines (the primary cell line SW480 and its LN metastatic variant, SW620) and identified eight genes [S100 calcium-binding protein P; aldo-keto reductase family 1(AKR1), member B1 (aldose reductase; AKR1B1); AKR1, member C3 (AKR1C3); calponin 3, acidic; metastasis associated in colon cancer 1; hemoglobin, epsilon 1; trefoil factor 3; and FGGY carbohydrate kinase domain containing]. These genes were examined by quantitative RT-PCR in tissues and LNs in 14 CRC patients and 11 control patients. The level of AKR1C3 mRNA expression was significantly different between the Dukes' stage A, B, and C groups and the control group (p < 0.05, p < 0.001, and p < 0.001) and was also significantly different between Dukes' stage C and A or B groups (p < 0.05 and p < 0.001, respectively). The expression of CNN3 was significantly different between the Dukes' stage C and B or control groups (p < 0.001 and p < 0.01, respectively). There were significant correlations between the expression levels of AKR1C3 and CNN3. AKR1C3 and CNN3 expressions are more accurate and suitable markers for the diagnosis of LN metastasis than the other six genes examined in this study.
authors.affiliation: Department of Biophysics, Kobe University Graduate School of Health Sciences, 7-10-2, Tomogaoka, Suma-ku, Kobe, 654-0142, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Nakarai
authors.forename: Kayo
authors.initials: K
authors.lastname: Osawa
authors.forename: Minami
authors.initials: M
authors.lastname: Akiyama
authors.forename: Nagahide
authors.initials: N
authors.lastname: Matsubara
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ikeuchi
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yamano
authors.forename: Seiichi
authors.initials: S
authors.lastname: Hirota
authors.forename: Naohiro
authors.initials: N
authors.lastname: Tomita
authors.forename: Makoto
authors.initials: M
authors.lastname: Usami
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kido
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis*; D000074425:Aldo-Keto Reductase Family 1 Member C3; D014408:Biomarkers, Tumor / Q000032:analysis*; D002478:Cells, Cultured; D015179:Colorectal Neoplasms / Q000473:pathology* / Q000556:secondary*; D016213:Cyclins / Q000032:analysis*; D020869:Gene Expression Profiling; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000032:analysis*; D008198:Lymph Nodes / Q000473:pathology*; D009362:Neoplasm Metastasis / Q000175:diagnosis* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C000597250:CNNM3 protein, human; D016213:Cyclins; D015096:3-Hydroxysteroid Dehydrogenases; D006911:Hydroxyprostaglandin Dehydrogenases; C487341:AKR1C3 protein, human; D000074425:Aldo-Keto Reductase Family 1 Member C3