title: Involvement of PKA, PKC, CAMK-II and MEK1/2 in the acute antidepressant-like effect of creatine in mice.
abstract: BACKGROUND
  The aim of this study was to investigate the involvement of signaling pathways on the creatine antidepressant-like effect in the tail suspension test (TST) in mice.
  
  
  METHODS
  The TST was used to assess the antidepressant-like properties of creatine.
  
  
  RESULTS
  The anti-immobility effect of creatine (1mg/kg, p.o.) in the TST was blocked by i.c.v. pretreatment with H-89 (1μg/site, PKA inhibitor), KN-62 (1μg/site, CAMK-II inhibitor), chelerythrine (1μg/site, PKC inhibitor), U0126 (5μg/site, MEK1/2 inhibitor) or PD09058 (5μg/site, MEK1/2 inhibitor).
  
  
  CONCLUSIONS
  These results suggest that the antidepressant-like effect of creatine is dependent on PKA, CaMK-II, PKC and MEK 1/2 activation.
authors.affiliation: Department of Biochemistry, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
authors.forename: Mauricio P
authors.initials: MP
authors.lastname: Cunha
authors.affiliation: Department of Biochemistry, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
authors.forename: Josiane
authors.initials: J
authors.lastname: Budni
authors.affiliation: Department of Biochemistry, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
authors.forename: Francis L
authors.initials: FL
authors.lastname: Pazini
authors.affiliation: Department of Biochemistry, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
authors.forename: Ágatha
authors.initials: Á
authors.lastname: Oliveira
authors.affiliation: Department of Biochemistry, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
authors.forename: Julia M
authors.initials: JM
authors.lastname: Rosa
authors.affiliation: Department of Biochemistry, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
authors.forename: Mark W
authors.initials: MW
authors.lastname: Lopes
authors.affiliation: Department of Biochemistry, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
authors.forename: Rodrigo B
authors.initials: RB
authors.lastname: Leal
authors.affiliation: Department of Biochemistry, Universidade Federal de Santa Catarina, Florianópolis, Brazil. Electronic address: alsrodri@gmail.com.
authors.forename: Ana Lúcia S
authors.initials: AL
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D001522:Behavior, Animal / Q000187:drug effects; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism; D003401:Creatine / Q000008:administration & dosage / Q000627:therapeutic use*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004195:Disease Models, Animal; D019417:Hindlimb Suspension; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D009043:Motor Activity / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013315:Stress, Psychological / Q000188:drug therapy* / Q000201:enzymology*
chemical_list: D000928:Antidepressive Agents; D047428:Protein Kinase Inhibitors; D017346:Protein Serine-Threonine Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D020929:Mitogen-Activated Protein Kinase Kinases; D003401:Creatine
keywords: Antidepressant; Creatine; Intracellular signaling; Kinases; Tail suspension test