title: Application of response surface methodology for determination of methyl red in water samples by spectrophotometry method.
abstract: In this study a rapid and effective method (dispersive liquid-liquid microextraction (DLLME)) was developed for extraction of methyl red (MR) prior to its determination by UV-Vis spectrophotometry. Influence variables on DLLME such as volume of chloroform (as extractant solvent) and methanol (as dispersive solvent), pH and ionic strength and extraction time were investigated. Then significant variables were optimized by using a Box-Behnken design (BBD) and desirability function (DF). The optimized conditions (100μL of chloroform, 1.3mL of ethanol, pH 4 and 4% (w/v) NaCl) resulted in a linear calibration graph in the range of 0.015-10.0mgmL(-1) of MR in initial solution with R(2)=0.995 (n=5). The limits of detection (LOD) and limit of quantification (LOQ) were 0.005 and 0.015mgmL(-1), respectively. Finally, the DLLME method was applied for determination of MR in different water samples with relative standard deviation (RSD) less than 5% (n=5).
authors.affiliation: Department of Chemistry, Yasouj University, Yasouj 75914-35, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Khodadoust
authors.affiliation: Department of Chemistry, Yasouj University, Yasouj 75914-35, Iran. Electronic address: m_ghaedi@mail.yu.ac.ir.
authors.forename: Mehrorang
authors.initials: M
authors.lastname: Ghaedi
mesh_terms: D001391:Azo Compounds / Q000032:analysis* / Q000302:isolation & purification; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods*; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D001391:Azo Compounds; D014874:Water Pollutants, Chemical; D014867:Water; C008492:methyl red
keywords: Box–Behnken design; Desirability function; Dispersive liquid–liquid microextraction; Methyl red