title: Chemically defined generation of human cardiomyocytes.
abstract: Existing methods for human induced pluripotent stem cell (hiPSC) cardiac differentiation are efficient but require complex, undefined medium constituents that hinder further elucidation of the molecular mechanisms of cardiomyogenesis. Using hiPSCs derived under chemically defined conditions on synthetic matrices, we systematically developed an optimized cardiac differentiation strategy, using a chemically defined medium consisting of just three components: the basal medium RPMI 1640, L-ascorbic acid 2-phosphate and rice-derived recombinant human albumin. Along with small molecule-based induction of differentiation, this protocol produced contractile sheets of up to 95% TNNT2(+) cardiomyocytes at a yield of up to 100 cardiomyocytes for every input pluripotent cell and was effective in 11 hiPSC lines tested. This chemically defined platform for cardiac specification of hiPSCs will allow the elucidation of cardiomyocyte macromolecular and metabolic requirements and will provide a minimal system for the study of maturation and subtype specification.
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Paul W
authors.initials: PW
authors.lastname: Burridge
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Elena
authors.initials: E
authors.lastname: Matsa
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Praveen
authors.initials: P
authors.lastname: Shukla
authors.affiliation: Department of Applied Physics, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Ziliang C
authors.initials: ZC
authors.lastname: Lin
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Jared M
authors.initials: JM
authors.lastname: Churko
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Antje D
authors.initials: AD
authors.lastname: Ebert
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Lan
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Diecke
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Bruno
authors.initials: B
authors.lastname: Huber
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Mordwinkin
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Jordan R
authors.initials: JR
authors.lastname: Plews
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Oscar J
authors.identifier: 000000029991400X
authors.initials: OJ
authors.lastname: Abilez
authors.affiliation: Department of Chemistry, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Bianxiao
authors.initials: B
authors.lastname: Cui
authors.affiliation: Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Gold
authors.affiliation: 1] Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. [2] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA. [3] Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Joseph C
authors.initials: JC
authors.lastname: Wu
mesh_terms: D002454:Cell Differentiation; D003470:Culture Media; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D032383:Myocytes, Cardiac / Q000166:cytology*
chemical_list: D003470:Culture Media