title: Identification of the in vivo function of the high-efficiency D-mannonate dehydratase in Caulobacter crescentus NA1000 from the enolase superfamily.
abstract: The d-mannonate dehydratase (ManD) subgroup of the enolase superfamily contains members with varying catalytic activities (high-efficiency, low-efficiency, or no activity) that dehydrate d-mannonate and/or d-gluconate to 2-keto-3-deoxy-d-gluconate [Wichelecki, D. J., et al. (2014) Biochemistry 53, 2722-2731]. Despite extensive in vitro characterization, the in vivo physiological role of a ManD has yet to be established. In this study, we report the in vivo functional characterization of a high-efficiency ManD from Caulobacter crescentus NA1000 (UniProt entry B8GZZ7) by in vivo discovery of its essential role in d-glucuronate metabolism. This in vivo functional annotation may be extended to ~50 additional proteins.
authors.affiliation: Institute for Genomic Biology, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Wichelecki
authors.forename: Dylan C
authors.initials: DC
authors.lastname: Graff
authors.forename: Nawar
authors.initials: N
authors.lastname: Al-Obaidi
authors.forename: Steven C
authors.initials: SC
authors.lastname: Almo
authors.forename: John A
authors.initials: JA
authors.lastname: Gerlt
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016935:Caulobacter crescentus / Q000378:metabolism*; D055786:Gene Knockout Techniques; D016680:Genome, Bacterial; D020723:Glucuronic Acid / Q000378:metabolism; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D001426:Bacterial Proteins; D020723:Glucuronic Acid; D006836:Hydro-Lyases; D010751:Phosphopyruvate Hydratase; C021545:mannonate dehydratase