title: Human adipose tissue derived pericytes increase life span in Utrn (tm1Ked) Dmd (mdx) /J mice.
abstract: Duchenne muscular dystrophy (DMD) is still an untreatable lethal X-linked disorder, which affects 1 in 3500 male births. It is caused by the absence of muscle dystrophin due to mutations in the dystrophin gene. The potential regenerative capacity as well as immune privileged properties of mesenchymal Stem Cells (MSC) has been under investigation for many years in an attempt to treat DMD. One of the questions to be addressed is whether stem cells from distinct sources have comparable clinical effects when injected in murine or canine muscular dystrophy animal models. Many studies comparing different stem cells from various sources were reported but these cells were obtained from different donors and thus with different genetic backgrounds. Here we investigated whether human pericytes obtained from 4 different tissues (muscle, adipose tissue, fallopian tube and endometrium) from the same donor have a similar clinical impact when injected in double mutant Utrn (tm1Ked) Dmd (mdx) /J mice, a clinically relevant model for DMD. After a weekly regimen of intraperitoneal injections of 10(6) cells per 8 weeks we evaluated the motor ability as well as the life span of the treated mice as compared to controls. Our experiment showed that only adipose tissue derived pericytes are able to increase significantly (39 days on average) the life span of affected mice. Microarray analysis showed an inhibition of the interferon pathway by adipose derived pericytes. Our results suggest that the clinical benefit associated with intraperitoneal injections of these adult stem cells is related to immune modulation rather than tissue regeneration.
authors.affiliation: Human Genome and stem-cell Center, Institute of Biosciences, University of São Paulo, Rua do Matāo 106, São Paulo, CEP 05508-030, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Valadares
authors.forename: J P
authors.initials: JP
authors.lastname: Gomes
authors.forename: G
authors.initials: G
authors.lastname: Castello
authors.forename: A
authors.initials: A
authors.lastname: Assoni
authors.forename: M
authors.initials: M
authors.lastname: Pellati
authors.forename: C
authors.initials: C
authors.lastname: Bueno
authors.forename: M
authors.initials: M
authors.lastname: Corselli
authors.forename: H
authors.initials: H
authors.lastname: Silva
authors.forename: P
authors.initials: P
authors.lastname: Bartolini
authors.forename: M
authors.initials: M
authors.lastname: Vainzof
authors.forename: P F
authors.initials: PF
authors.lastname: Margarido
authors.forename: E
authors.initials: E
authors.lastname: Baracat
authors.forename: B
authors.initials: B
authors.lastname: Péault
authors.forename: M
authors.initials: M
authors.lastname: Zatz
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D016189:Dystrophin / Q000378:metabolism; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology; D051379:Mice; D018101:Mice, Inbred mdx; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology; D020388:Muscular Dystrophy, Duchenne / Q000378:metabolism / Q000628:therapy; D020286:Pericytes / Q000378:metabolism / Q000502:physiology*
chemical_list: D016189:Dystrophin