title: Expression of Ki-67, Bax and p73 in patients with hilar cholangiocarcinoma.
abstract: BACKGROUND
  Hilar cholangiocarcinoma (HCC) is defined as a cholangiocarcinoma located in the bifurcation of the right and left bile duct, constituting 40%-60% of all reported cholangiocarcinoma and 58%-75% of the extrahepatic cholangiocarcinoma. In this study, we aim to investigate the expression of Ki-67, Bax and p73 in the patients with HCC, and identify their potential roles in the prognosis of HCC.
  
  
  OBJECTIVE
  Thirty five HCC patients (male: 25, female: 10) with an average age of 62.8 years were diagnosed with HCC according to the pathological tests. A total of 20 cadavers with normal hilar bile ducts were used as control.
  
  
  METHODS
  The expression of Ki-67, Bax, and p73 was determined using immunohistochemical analysis.
  
  
  RESULTS
  The pathological test indicated that Ki-67 expression increased with the stage of the disease, and the infiltration of the cancer cells. Statistical difference was noted in the expression of Bax LI between the patients with HCC and normal control (32.4 ± 17.6 vs 11.2 ± 7.9, P< 0.01). Statistical difference was observed between the patients with or without lymphatic metastasis, and those of various differentiation stages (P < 0.05). Among the 35 patients with HCC, expression of p73 was observed in 11 patients (31.43%). Compared with normal control, remarkable increase of p73 expression was noted in the HCC patients (P < 0.01). Additionally, no statistical difference was noted in the expression of p73 in the patients with various disease stages, and those with or without metastasis (P> 0.05).
  
  
  CONCLUSIONS
  Ki-67, Bax, and p73 could be used as biomarkers for the prognosis of HCC.
authors.affiliation: Department of General Surgery (Division II), the Affiliated Hospital of Medical College Qingdao University, Qingdao, Shandong, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of General Surgery (Division II), the Affiliated Hospital of Medical College Qingdao University, Qingdao, Shandong, China.
authors.forename: Bingyuan
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Cardiovascular Center, Qingdao Fuwai Cardiovascular Hospital, Qingdao, Shandong, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of General Surgery (Division II), the Affiliated Hospital of Medical College Qingdao University, Qingdao, Shandong, China.
authors.forename: Xianxiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of General Surgery (Division II), the Affiliated Hospital of Medical College Qingdao University, Qingdao, Shandong, China.
authors.forename: Jilin
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of General Surgery (Division II), the Affiliated Hospital of Medical College Qingdao University, Qingdao, Shandong, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of General Surgery (Division II), the Affiliated Hospital of Medical College Qingdao University, Qingdao, Shandong, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Lu
mesh_terms: D001650:Bile Duct Neoplasms / Q000378:metabolism*; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D018281:Cholangiocarcinoma / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011379:Prognosis; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis*; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis*
chemical_list: C494826:BAX protein, human; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; D051028:bcl-2-Associated X Protein
keywords: Bax; Hilar cholangiocarcinoma; Ki-67; p73; prognosis