title: Mixed reverse micelles facilitated downstream processing of lipase involving water-oil-water liquid emulsion membrane.
abstract: Our earlier work for the first time demonstrated that liquid emulsion membrane (LEM) containing reverse micelles could be successfully used for the downstream processing of lipase from Aspergillus niger. In the present work, we have attempted to increase the extraction and purification fold of lipase by using mixed reverse micelles (MRM) consisting of cationic and nonionic surfactants in LEM. It was basically prepared by addition of the internal aqueous phase solution to the organic phase followed by the redispersion of the emulsion in the feed phase containing enzyme, which resulted in globules of water-oil-water (WOW) emulsion for the extraction of lipase. The optimum conditions for maximum lipase recovery (100%) and purification fold (17.0-fold) were CTAB concentration 0.075 M, Tween 80 concentration 0.012 M, at stirring speed of 500 rpm, contact time 15 min, internal aqueous phase pH 7, feed pH 9, KCl concentration 1 M, NaCl concentration 0.1 M, and ratio of membrane emulsion to feed volume 1:1. Incorporation of the nonionic surfactant (e.g., Tween 80) resulted in remarkable improvement in the purification fold (3.1-17.0) of the lipase. LEM containing a mixture of nonionic and cationic surfactants can be successfully used for the enhancement in the activity recovery and purification fold during downstream processing of enzymes/proteins.
authors.affiliation: Dept. of Food Engineering, CSIR-Central Food Technological Research Inst., Mysore, Karnataka, 570 020, India.
authors.forename: Saibal
authors.initials: S
authors.lastname: Bhowal
authors.forename: B S
authors.initials: BS
authors.lastname: Priyanka
authors.forename: Navin K
authors.initials: NK
authors.lastname: Rastogi
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D008567:Membranes, Artificial; D008823:Micelles; D011136:Polysorbates / Q000737:chemistry; D011189:Potassium Chloride / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013997:Time Factors; D014867:Water
chemical_list: D002593:Cetrimonium Compounds; D004655:Emulsions; D008567:Membranes, Artificial; D008823:Micelles; D011136:Polysorbates; D013501:Surface-Active Agents; D014867:Water; D012965:Sodium Chloride; D011189:Potassium Chloride; D008049:Lipase; D000077286:Cetrimonium
keywords: cationic surfactant; downstream processing; lipase; liquid emulsion membrane; nonionic surfactant; reverse micelles