title: A modified GEWF solution is cost-saving and effective for lymph node retrieval in resected colorectal carcinoma specimens.
abstract: Lymph node (LN) retrieval is important for proper staging of colorectal carcinoma. Although various assistant techniques were recommended to facilitate LN identification, most of them were unavoidably time-consuming, resource intensive and costly. We prepared a modified GEWF solution (RE-GEWF) by use of recycled alcohol and a familiar dye, eosin and investigated its efficacy on 55 colorectal carcinoma specimens. Of the 55 studied cases, 33 of them with <12 LNs (Group A) and 22 with ≥12 LNs were detected (Group B) before RE-GEWF treatment. All were subsequently treated with RE-GEWF for 14-16h and were inspected again for LNs. The number of LNs revealed before and after RE-GEWF treatment was 539 and 476 respectively. The mean number of LNs per cases increased from 9.80±6.27 to 18.43±8.77. Twelve accessory LN metastases were found in 9 cases. Upgrade of pN stage was only present in 7 of the Group A cases. The results show that RE-GEWF is as effective as other reported LN revealing solutions. Use of RE-GEWF not only can assure the quality of LN detection, but also minimize the cost and reduce the release of waste.
authors.affiliation: Department of Pathology, Peking University Third Hospital, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Ma
authors.affiliation: Department of Pathology, Peking University Third Hospital, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Ju-Xiang
authors.initials: JX
authors.lastname: Ye
authors.affiliation: Department of Pathology, Peking University Third Hospital, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Pathology, Peking University Third Hospital, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Qi
authors.affiliation: Department of Pathology, Peking University Third Hospital, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Qing-Yang
authors.initials: QY
authors.lastname: Meng
authors.affiliation: Department of Pathology, Peking University Third Hospital, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China. Electronic address: shixueying@bjmu.edu.cn.
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Shi
mesh_terms: D019342:Acetic Acid; D015179:Colorectal Neoplasms / Q000473:pathology*; D004801:Eosine Yellowish-(YS); D000431:Ethanol; D005557:Formaldehyde; D006801:Humans; D008197:Lymph Node Excision / Q000191:economics / Q000379:methods*; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis / Q000473:pathology*; D012996:Solutions / Q000191:economics
chemical_list: D012996:Solutions; D005557:Formaldehyde; D000431:Ethanol; D019342:Acetic Acid; D004801:Eosine Yellowish-(YS)
keywords: Colorectal carcinoma; Eosin; Lymph node revealing solution; Recycled alcohol; Staging