title: Towards rapid genotyping of resistant malaria parasites: could loop-mediated isothermal amplification be the solution?
abstract: Loop-mediated isothermal amplification (LAMP) is an innovative molecular technique that has been validated for point-of-care testing to diagnose malaria. Molecular detection and tracking of anti-malarial drug resistance is mainly based on highly sophisticated, costly and time-consuming techniques. With the validation of resistance-associated gene mutations in malaria parasites, there is a need to develop rapid, easy-to-use molecular tests for anti-malarial drug resistance genotyping. LAMP could be further developed as a point-of-care test to rapidly detect anti-malarial drug resistance-associated molecular markers, thereby help detecting and monitoring drug resistance in surveillance studies.
authors.affiliation: Department of Parasitology, Faculty of Medicine and Health Sciences, Sana'a University, Sana'a, Yemen. rashadqb@yahoo.com.
authors.forename: Rashad
authors.initials: R
authors.lastname: Abdul-Ghani
mesh_terms: D004351:Drug Resistance; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D008288:Malaria / Q000469:parasitology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D021261:Parasitic Sensitivity Tests / Q000379:methods; D010961:Plasmodium / Q000187:drug effects* / Q000235:genetics*; D019095:Point-of-Care Systems