title: Extracellular nucleotide catabolism in aortoiliac bifurcation of atherosclerotic ApoE/LDLr double knock out mice.
abstract: Atherosclerosis is a consequence of diverse pathologies that could be affected by signaling mediated by nucleotides and their metabolites. Concentration of specific nucleotide derivatives in the proximity of purinergic receptors is controlled by extracellular enzymes such as ecto-nucleoside triphopsphate diphosphohydrolase (eNTPD), ecto-5'-nucleotidase (e5NT), and ecto-adenosine deaminase (eADA). To estimate changes in metabolism of extracellular nucleotides in the atherosclerotic vessel wall, aortoiliac bifurcation of ApoE/LDLr (-/-) mice was perfused with solution containing adenosine-5'-triphosphate (ATP), adenosine-5'-monophosphate (AMP) or adenosine. Formation of the product of eNTPD, e5NT or eADA was measured by high performance liquid chromatography (HPLC). The most significant difference between ApoE/LDLr (-/-) and wild-type mice was several times higher rate of conversion of adenosine to inosine catalyzed by eADA activity. This highlights potential decrease in intravascular adenosine concentration in atherosclerosis.
authors.affiliation: a Department of Biochemistry , Medical University of Gdansk , Gdansk , Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kutryb-Zajac
authors.forename: Paulina
authors.initials: P
authors.lastname: Zukowska
authors.forename: Marta
authors.initials: M
authors.lastname: Toczek
authors.forename: Magdalena
authors.initials: M
authors.lastname: Zabielska
authors.forename: Marcin
authors.initials: M
authors.lastname: Lipinski
authors.forename: Iwona
authors.initials: I
authors.lastname: Rybakowska
authors.forename: Stefan
authors.initials: S
authors.lastname: Chlopicki
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Slominska
authors.forename: Ryszard T
authors.initials: RT
authors.lastname: Smolenski
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000473:pathology*; D001057:Apolipoproteins E / Q000172:deficiency* / Q000235:genetics; D050197:Atherosclerosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005110:Extracellular Space / Q000201:enzymology / Q000378:metabolism*; D007083:Iliac Artery / Q000473:pathology*; D051379:Mice; D018345:Mice, Knockout; D009711:Nucleotides / Q000378:metabolism*; D011973:Receptors, LDL / Q000172:deficiency* / Q000235:genetics
chemical_list: D001057:Apolipoproteins E; D009711:Nucleotides; D011973:Receptors, LDL; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D000241:Adenosine
keywords: Nucleotides; adenosine; adenosine deaminase; atherosclerosis; ecto-5′-nucleotidase; ecto-nucleoside triphopsphate diphosphohydrolase; inflammation