title: Passion fruit peel extract attenuates bleomycin-induced pulmonary fibrosis in mice.
abstract: Idiopathic pulmonary fibrosis is a progressive fatal lung disease characterized by excessive collagen deposition, with no effective treatments. We investigated the efficacy of natural products with high anti-inflammatory activity, such as passion fruit peel extract (PFPE), in a mouse model of bleomycin-induced pulmonary fibrosis (PF). C57BL/6J mice were subjected to a single intratracheal instillation of bleomycin to induce PF. Daily PFPE treatment significantly reduced loss of body mass and mortality rate in mice compared with those treated with bleomycin. While bleomycin-induced PF resulted in elevated total numbers of inflammatory cells, macrophages, lymphocytes, and neutrophils in bronchoalveolar lavage fluid on both days 7 and 21, PFPE administration significantly attenuated these phenomena compared with bleomycin group. On day 7, the decreased superoxide dismutase and myeloperoxidase activities observed in the bleomycin group were significantly restored with PFPE treatment. On day 21, enhanced hydroxyproline deposition in the bleomycin group was also suppressed by PFPE administration. PFPE treatment significantly attenuated extensive inflammatory cell infiltration and accumulation of collagen in lung tissue sections of bleomycin-induced mice on days 7 and 21, respectively. Our results indicate that administration of PFPE decreased bleomycin-induced PF because of anti-inflammatory and antioxidant activities.
authors.affiliation: a SugenLife Sciences Pvt. Ltd., Tirupati, Andhra Pradesh 517 505, India.
authors.forename: Shanmuga Reddy
authors.initials: SR
authors.lastname: Chilakapati
authors.forename: Mamatha
authors.initials: M
authors.lastname: Serasanambati
authors.forename: Pavan Kumar
authors.initials: PK
authors.lastname: Manikonda
authors.forename: Damodar Reddy
authors.initials: DR
authors.lastname: Chilakapati
authors.forename: Ronald Ross
authors.initials: RR
authors.lastname: Watson
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000903:Antibiotics, Antineoplastic / Q000009:adverse effects*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001761:Bleomycin / Q000009:adverse effects*; D001835:Body Weight / Q000187:drug effects; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D005260:Female; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use; D006909:Hydroxyproline / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D029598:Passiflora / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000188:drug therapy* / Q000401:mortality; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D001761:Bleomycin; D009195:Peroxidase; D013482:Superoxide Dismutase; D006909:Hydroxyproline
keywords: bleomycin; bléomycine; bronchoalveolar lavage fluid; fibrose pulmonaire idiopathique; fruit de la passion; idiopathic pulmonary fibrosis; liquide de lavage broncho-alvéolaire; myeloperoxidase; myéloperoxydase; passion fruit; superoxide dismutase; superoxyde dismutase