title: Cubic B-spline calibration for 3D super-resolution measurements using astigmatic imaging.
abstract: In recent years three-dimensional (3D) super-resolution fluorescence imaging by single-molecule localization (localization microscopy) has gained considerable interest because of its simple implementation and high optical resolution. Astigmatic and biplane imaging are experimentally simple methods to engineer a 3D-specific point spread function (PSF), but existing evaluation methods have proven problematic in practical application. Here we introduce the use of cubic B-splines to model the relationship of axial position and PSF width in the above mentioned approaches and compare the performance with existing methods. We show that cubic B-splines are the first method that can combine precision, accuracy and simplicity.
authors.forename: Sven
authors.initials: S
authors.lastname: Proppert
authors.forename: Steve
authors.initials: S
authors.lastname: Wolter
authors.forename: Thorge
authors.initials: T
authors.lastname: Holm
authors.forename: Teresa
authors.initials: T
authors.lastname: Klein
authors.forename: Sebastian
authors.initials: S
authors.lastname: van de Linde
authors.forename: Markus
authors.initials: M
authors.lastname: Sauer