title: Vaccination with a genetically modified Brugia malayi cysteine protease inhibitor-2 reduces adult parasite numbers and affects the fertility of female worms following a subcutaneous challenge of Mongolian gerbils (Meriones unguiculatus) with B. malayi infective larvae.
abstract: Vaccination of Mongolian gerbils with Brugia malayi cysteine protease inhibitor-2 in which the amino acid Asn66 was mutated to Lys66 (Bm-CPI-2M) resulted in reduced parasite numbers of 48.6% and 48.0% at 42 and 90 days p.i. with B. malayi L3s. Fertility of female worms was also affected at 90 days p.i. In vitro killing of L3s observed in the presence of gerbil peritoneal exudate cells and anti-Bm-CPI-2M sera suggests antibody-dependent cell-mediated cytotoxicity as a putative protective mechanism. These observations suggest that Bm-CPI-2M is a promising prophylactic and anti-fecundity vaccine candidate.
authors.affiliation: Department of Pathobiological Sciences, LSU School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA.
authors.forename: Sridhar
authors.initials: S
authors.lastname: Arumugam
authors.affiliation: Department of Pediatrics and Tropical Medicine, Baylor College of Medicine, Texas Children Hospital, 1102 Bates St., Ste.550, Houston, TX 77030, USA.
authors.forename: Junfei
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Pathobiological Sciences, LSU School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA.
authors.forename: Danielle
authors.initials: D
authors.lastname: Ward
authors.affiliation: Department of Microbiology and Immunology, Thomas Jefferson University, 233 S. 10th Street, Philadelphia, PA 19107, USA.
authors.forename: David
authors.initials: D
authors.lastname: Abraham
authors.affiliation: Laboratory of Molecular Parasitology, Lindsley F. Kimball Research Institute, New York Blood Center, 310 E 67th St., New York, NY 10065, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Lustigman
authors.affiliation: Department of Pediatrics and Tropical Medicine, Baylor College of Medicine, Texas Children Hospital, 1102 Bates St., Ste.550, Houston, TX 77030, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhan
authors.affiliation: Department of Pathobiological Sciences, LSU School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA. Electronic address: tklei1@lsu.edu.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Klei
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D017178:Brugia malayi / Q000235:genetics* / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005368:Filariasis / Q000469:parasitology* / Q000517:prevention & control*; D005786:Gene Expression Regulation; D005849:Gerbillinae / Q000469:parasitology*; D007814:Larva / Q000276:immunology; D014612:Vaccines / Q000276:immunology*
chemical_list: D015853:Cysteine Proteinase Inhibitors; D014612:Vaccines
keywords: Antibody dependent cell-mediated cytotoxicity (ADCC); Brugia malayi cysteine protease inhibitor-2 (Bm-CPI-2); Fecundity; Filariasis; Vaccination