title: Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces.
abstract: Recombinant human gelatin was conjugated with dopamine using carbodiimide as a surface modifier. This dopamine-coupled human gelatin (D-rhG) was characterized by (1)H-nuclear magnetic resonance, mass spectroscopy, and circular dichroism. D-rhG-coated surface properties were analyzed by physicochemical methods. Additionally, cell attachment and growth on the modified surfaces was assessed using human umbilical endothelial cells. Binding of gelatin onto titanium was significantly enhanced by dopamine conjugation. The thickness of the D-rhG coating depended on the treatment pH; thicker layers were formed at higher pH values, with a maximum thickness of 30 nm. D-rhG enhanced the binding of collagen-binding vascular endothelial growth factor and cell adhesion as compared with gelatin alone, even at the same surface concentration. The D-rhG surface modifier enhanced substrate binding by creating an adhesive nanointerface that increased specific protein binding and cell attachment.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Saitama, Japan ; School of Pharmaceutical Sciences, Jilin University, Jilin, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Saitama, Japan.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Saitama, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Tada
authors.affiliation: Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, Saitama.
authors.forename: Di
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Saitama, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kitajima
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Saitama, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Isoshima
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Saitama, Japan ; Department of Biomaterials and Bioengineering, Graduate School of Dental Medicine, Hokkaido University, Hokkaido.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshida
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Saitama, Japan ; Dental Hygiene Program, Kibi International College, Okayama, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Nakamura
authors.affiliation: School of Pharmaceutical Sciences, Jilin University, Jilin, People's Republic of China.
authors.forename: Weiqun
authors.initials: W
authors.lastname: Yan
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Saitama, Japan ; Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, Saitama.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000269:Adhesives / Q000138:chemical synthesis* / Q000494:pharmacology*; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D049872:Bivalvia / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004298:Dopamine / Q000737:chemistry / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology*; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing
chemical_list: D000269:Adhesives; D005780:Gelatin; D004298:Dopamine
keywords: cell adhesion; cell culture; dopamine; natural catechols; recombinant human gelatin; titanium