title: Biosynthesis of gold nanoparticles using Sargassum swartzii and its cytotoxicity effect on HeLa cells.
abstract: In this investigation, biological synthesis of gold nanoparticles (AuNPs) using Sargassum swartzii and its cytotoxicity against human cervical carcinoma (HeLa) cells is reported. The biological synthesis involved the reduction of chloroauric acid led to the formation of AuNPs within 5min at 60°C and the formation of AuNPs was confirmed using UV-vis spectrophotometer. The AuNPs were stable; spherical in shape with well-defined dimensions, and the average size of the particle is 35nm. A zeta potential value of -27.6mV revealed synthesized AuNPs were highly stable. The synthesized AuNPs exhibited a dose-dependent cytotoxicity against human cervical carcinoma (HeLa) cells. Furthermore, induction of apoptosis was measured by DAPI (4',6-Diamidino-2-phenylindole dihydrochloride) staining.
authors.affiliation: Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: T Stalin
authors.initials: TS
authors.lastname: Dhas
authors.affiliation: Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India. Electronic address: ganesv@gmail.com.
authors.forename: V Ganesh
authors.initials: VG
authors.lastname: Kumar
authors.affiliation: Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: V
authors.initials: V
authors.lastname: Karthick
authors.affiliation: Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: K
authors.initials: K
authors.lastname: Govindaraju
authors.affiliation: Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Kadiri 515 591, Andhra Pradesh, India.
authors.forename: T
authors.initials: T
authors.lastname: Shankara Narayana
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002584:Cervix Uteri / Q000187:drug effects / Q000473:pathology; D005260:Female; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology*; D055772:Green Chemistry Technology; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D044647:Sargassum / Q000737:chemistry*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D006046:Gold
keywords: Anticancer; Apoptosis; Gold nanoparticles; HeLa; Sargassum swartzii