title: An amorphous silicon photodiode microfluidic chip to detect nanomolar quantities of HIV-1 virion infectivity factor.
abstract: A hydrogenated amorphous silicon (a-Si:H) photosensor was explored for the quantitative detection of a HIV-1 virion infectivity factor (Vif) at a detection limit in the single nanomolar range. The a-Si:H photosensor was coupled with a microfluidic channel that was functionalized with a recombinant single chain variable fragment antibody. The biosensor selectively recognizes HIV-1 Vif from human cell extracts.
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Centre for Molecular and Structural Biomedicine (CBME), University of Algarve, Campus de Gambelas, 8000-139 Faro, Portugal. gferrei@ualg.pt.
authors.forename: Cláudia R
authors.initials: CR
authors.lastname: Vistas
authors.forename: Sandra S
authors.initials: SS
authors.lastname: Soares
authors.forename: Rogério M M
authors.initials: RM
authors.lastname: Rodrigues
authors.forename: Virginia
authors.initials: V
authors.lastname: Chu
authors.forename: João P
authors.initials: JP
authors.lastname: Conde
authors.forename: Guilherme N M
authors.initials: GN
authors.lastname: Ferreira
mesh_terms: D057809:HEK293 Cells; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D055095:Optics and Photonics / Q000295:instrumentation; D012825:Silicon / Q000737:chemistry*; D054320:vif Gene Products, Human Immunodeficiency Virus / Q000302:isolation & purification*
chemical_list: D054320:vif Gene Products, Human Immunodeficiency Virus; D012825:Silicon