title: Modulation of proteome expression by F-type lectin during viral hemorrhagic septicemia virus infection in fathead minnow cells.
abstract: Lectins found in fish tissues play an important role in the innate immune response against viral infection. A fucose-binding type lectin, RbFTL-3, from rock bream (Oplegnathus fasciatus) was identified using expressed sequence tag (EST) analysis. The expression of RbFTL-3 mRNA was higher in intestine than other tissues of rock bream. To determine the function of RbFTL-3, VHSV-susceptible fathead minnow (FHM) cells were transfected with pcDNA3.1(+) or pcDNA3.1(+)-RbFTL-3 and further infected with VHSV. The results show that the viability of FHM cells transfected with pcDNA3.1(+)-RbFTL-3 is higher than that of cells transfected with pcDNA3.1(+) (relative cell viability: 28.9% vs 56.2%). A comparative proteomic analysis, performed to explore the proteins related to the protective effect of RbFTL-3 in the cells during VHSV infection, identified 90 proteins differentially expressed in VHSV-infected FHM cells transfected with pcDNA3.1(+) or pcDNA3.1(+)-RbFTL-3. The expression of RbFTL-3 inhibits a vascular-sorting protein (SNF8) and diminishes the loss of prothrombin, which are closely associated with controlling viral budding and hemorrhage in fish cells, respectively. Subsequent Ingenuity Pathways Analysis enabled prediction of their biofunctional groupings and interaction networks. The results suggest RbFTL-3 modulates the expression of proteins related to viral budding (SNF8, CCT5 and TUBB) and thrombin signaling (F2) to increase the viability of VHSV infected cells.
authors.affiliation: Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Cho
authors.affiliation: Korea Basic Science Institute, Daejeon 305-806, South Korea.
authors.forename: Joseph
authors.initials: J
authors.lastname: Kwon
authors.affiliation: Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Bipin
authors.initials: B
authors.lastname: Vaidya
authors.affiliation: Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Jeonnam, South Korea.
authors.forename: Jong-Oh
authors.initials: JO
authors.lastname: Kim
authors.affiliation: Personal Genomics Institute, Genome Research Foundation, Suwon 443-270, South Korea.
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Eun-Hye
authors.initials: EH
authors.lastname: Jeong
authors.affiliation: Korea Basic Science Institute, Daejeon 305-806, South Korea.
authors.forename: Keun Sik
authors.initials: KS
authors.lastname: Baik
authors.affiliation: Korea Basic Science Institute, Daejeon 305-806, South Korea.
authors.forename: Jong-Soon
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, South Korea; Bioenergy Research Center, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Hyeun-Jong
authors.initials: HJ
authors.lastname: Bae
authors.affiliation: Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Jeonnam, South Korea. Electronic address: ohmj@jnu.ac.kr.
authors.forename: Myung-Joo
authors.initials: MJ
authors.lastname: Oh
authors.affiliation: Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, Gwangju 500-757, South Korea; Bioenergy Research Center, Chonnam National University, Gwangju 500-757, South Korea. Electronic address: dwkim@jnu.ac.kr.
authors.forename: Duwoon
authors.initials: D
authors.lastname: Kim
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D003530:Cyprinidae; D017931:DNA Primers / Q000235:genetics; D056827:Endosomal Sorting Complexes Required for Transport / Q000037:antagonists & inhibitors; D020224:Expressed Sequence Tags; D005393:Fish Diseases / Q000276:immunology* / Q000821:virology*; D037102:Lectins / Q000378:metabolism* / Q000494:pharmacology; D013058:Mass Spectrometry; D029141:Novirhabdovirus; D020543:Proteome / Q000378:metabolism*; D011516:Prothrombin / Q000378:metabolism; D018353:Rhabdoviridae Infections / Q000276:immunology / Q000662:veterinary*; D014162:Transfection
chemical_list: D017931:DNA Primers; D056827:Endosomal Sorting Complexes Required for Transport; D037102:Lectins; D020543:Proteome; D011516:Prothrombin
keywords: Expressed sequence tag; F-type lectin; Proteomic analysis; Rock bream; Viral hemorrhagic septicemia