title: Formation of newly synthesized adeno-associated virus capsids in the cell nucleus.
abstract: Adeno-associated virus (AAV) particles inside the nucleus of a HEK 293 cell are shown by electron microscopy. Cells have been triple-transfected for vector production and were analyzed for capsid formation three days later. Newly assembled particle are visible as seemingly unstructured conglomerates or crystal-like arrays.
authors.affiliation: Gene Therapy Program, Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania , Philadelphia, PA 19104.
authors.forename: Peter
authors.initials: P
authors.lastname: Bell
authors.forename: Luk H
authors.initials: LH
authors.lastname: Vandenberghe
authors.forename: James M
authors.initials: JM
authors.lastname: Wilson
mesh_terms: D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism* / Q000821:virology*; D000229:Dependovirus / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008854:Microscopy, Electron
chemical_list: D036022:Capsid Proteins