title: Control of early seedling development by BES1/TPL/HDA19-mediated epigenetic regulation of ABI3.
abstract: Seed germination and young seedling establishment should be tightly regulated to maximize plant survival and thereby enable successful propagation. Plants have evolved developmental signalling networks to integrate environmental cues for proper control of these critical processes, in which brassinosteroids are known to attenuate ABA-mediated arrest of early seedling development; however, the underlying regulatory mechanism remains elusive. Here we reveal that a BES1/TPL/HDA19 repressor complex mediates the inhibitory action of brassinosteroids on ABA responses during early seedling development. BR-activated BES1 forms a transcriptional repressor complex with TPL-HDA19, which directly facilitates the histone deacetylation of ABI3 chromatin. This event leads to the transcriptional repression of ABI3 and consequently ABI5, major ABA signalling regulators in early seedling development. Our data reveal that the BR-activated BES1-TPL-HDA19 repressor complex controls epigenetic silencing of ABI3 and thereby suppresses the ABA signalling output during early seedling development.
authors.affiliation: 1] Department of Life Sciences, POSTECH Biotech Center, Pohang University of Science and Technology, Pohang 790-784, Korea [2] Division of Agricultural Microbiology, National Academy of Agricultural Science, RDA, Suwon 441-707, South Korea.
authors.forename: Hojin
authors.initials: H
authors.lastname: Ryu
authors.affiliation: Department of Life Sciences, POSTECH Biotech Center, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Hyunwoo
authors.initials: H
authors.lastname: Cho
authors.affiliation: Department of Life Sciences, POSTECH Biotech Center, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Wonsil
authors.initials: W
authors.lastname: Bae
authors.affiliation: Department of Life Sciences, POSTECH Biotech Center, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Ildoo
authors.initials: I
authors.lastname: Hwang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000378:metabolism / Q000502:physiology*; D060406:Brassinosteroids / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins; D044127:Epigenesis, Genetic / Q000502:physiology*; D018525:Germination / Q000502:physiology*; D006655:Histone Deacetylases / Q000378:metabolism; D015151:Immunoblotting; D046912:Multiprotein Complexes / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000254:growth & development*; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*
chemical_list: D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D004268:DNA-Binding Proteins; D006655:Histone Deacetylases; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D014157:Transcription Factors; C091601:ABI3 protein, Arabidopsis; C456160:BES1 protein, Arabidopsis; C511291:At1g15750 protein, Arabidopsis; C507152:HDA19 protein, Arabidopsis