title: Bactericidal effect of polyethyleneimine capped ZnO nanoparticles on multiple antibiotic resistant bacteria harboring genes of high-pathogenicity island.
abstract: Zinc oxide nanoparticles (ZnO-NP) were synthesized by alcoholic route using zinc acetate as the precursor material and lithium hydroxide as hydrolyzing agent. Further ZnO-PEI NP (derivative of ZnO-NP) was made in aqueous medium using the capping agent polyethyleneimine (PEI). The nanoparticles were characterized by XRD measurements, TEM and other techniques; the weight % of coating shell in the polymer-capped particles was determined by TGA. ZnO-PEI NP is more soluble in water than the uncapped ZnO-NP, and forms a colloidal suspension in water. PEI-capped ZnO-NP exhibited better antibacterial activity when compared with that of uncapped ZnO-NP against a range of multiple-antibiotic-resistant (MAR) Gram-negative bacterial strains harboring genes of high-pathogenicity island. ZnO-NP effectively killed these microorganisms by generating reactive oxygen species (ROS) and damaging bacterial membrane. ZnO-PEI NP at LD50 dose in combination with tetracycline showed synergistic effect to inhibit tetracycline-resistant Escherichia coli MREC33 growth by 80%. These results open up a new vista in therapeutics to use antibiotics (which have otherwise been rendered useless against MAR bacteria) in combination with minimized dosage of nanoparticles for the more effective control of MAR pathogenic bacteria.
authors.affiliation: Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VIIM, Kolkata 700054, India.
authors.forename: Soumyananda
authors.initials: S
authors.lastname: Chakraborti
authors.affiliation: Omics Laboratory, Department of Biotechnology, University of North Bengal, Siliguri 734013, West Bengal, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Mandal
authors.affiliation: Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VIIM, Kolkata 700054, India.
authors.forename: Shamila
authors.initials: S
authors.lastname: Sarwar
authors.affiliation: Omics Laboratory, Department of Biotechnology, University of North Bengal, Siliguri 734013, West Bengal, India.
authors.forename: Prashantee
authors.initials: P
authors.lastname: Singh
authors.affiliation: Omics Laboratory, Department of Biotechnology, University of North Bengal, Siliguri 734013, West Bengal, India. Electronic address: rcnbu2003@yahoo.com.
authors.forename: Ranadhir
authors.initials: R
authors.lastname: Chakraborty
authors.affiliation: Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VIIM, Kolkata 700054, India. Electronic address: pinak@jcbose.ac.in.
authors.forename: Pinak
authors.initials: P
authors.lastname: Chakrabarti
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001433:Bacteriolysis / Q000187:drug effects; D002417:Cattle; D053938:DNA Fragmentation / Q000187:drug effects; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D005798:Genes, Bacterial; D044404:Genomic Islands / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D053758:Nanoparticles / Q000648:ultrastructure; D011094:Polyethyleneimine / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013752:Tetracycline / Q000494:pharmacology; D015034:Zinc Oxide / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D017382:Reactive Oxygen Species; D012710:Serum Albumin, Bovine; D011094:Polyethyleneimine; D013752:Tetracycline; D015034:Zinc Oxide
keywords: Antibacterial activity; Multiple-antibiotic-resistant bacteria; PEI-capped ZnO nanoparticle; Reactive oxygen species; Synergistic effect of antibiotics