title: Genome sequences of porcine epidemic diarrhea virus: in vivo and in vitro phenotypes.
abstract: Since the outbreak of porcine epidemic diarrhea virus (PEDV) in May 2013, U.S. swine producers have lost almost five million baby pigs. In an attempt to understand the evolution of PEDV in the United States and possibly develop a control strategy, we compared the genome sequences of a PEDV strain isolated from an infected piglet against its in vitro adapted version. The original PEDV strain was grown in Vero cells and passed 10 times serially in a MARC145 cell line. The sequence analysis of the native PEDV strain and in vitro passaged virus shows that the cell culture adaptation specifically modifies PEDV spike protein whereas the open reading frame 1a/b (ORF1a/b)-encoded polyprotein, the nucleoprotein, NS3B (ORF3), and membrane and envelope proteins remain unchanged.
authors.affiliation: Newport Laboratories Inc., Worthington, Minnesota, USA plawrence@newportlabs.com.
authors.forename: Paulraj K
authors.initials: PK
authors.lastname: Lawrence
authors.affiliation: Newport Laboratories Inc., Worthington, Minnesota, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Bumgardner
authors.affiliation: Newport Laboratories Inc., Worthington, Minnesota, USA.
authors.forename: Russell F
authors.initials: RF
authors.lastname: Bey
authors.affiliation: Newport Laboratories Inc., Worthington, Minnesota, USA.
authors.forename: Douglas
authors.initials: D
authors.lastname: Stine
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington, USA.
authors.forename: Roger E
authors.initials: RE
authors.lastname: Bumgarner