title: Polyadenylation of Friend murine leukemia virus env-mRNA is affected by its splicing.
abstract: As splicing was previously found to be important for increasing Friend murine leukemia virus env-mRNA stability and translation, we investigated whether splicing of env-mRNA affected the poly(A) tail length using env expression vectors that yielded unspliced or spliced env-mRNA. Incomplete polyadenylation was detected in a fraction of the unspliced env-mRNA products in an env gene-dependent manner, showing that splicing of Friend murine leukemia virus plays an important role in the efficiency of complete polyadenylation of env-mRNA. These results suggested that the promotion of complete polyadenylation of env-mRNA by splicing might partially explain up-regulation of Env protein expression as a result of splicing.
authors.affiliation: Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236, Tangi-machi, Hachioji-shi, Tokyo, 192-8577, Japan.
authors.forename: Akihito
authors.initials: A
authors.lastname: Machinaga
authors.forename: Sayaka
authors.initials: S
authors.lastname: Takase-Yoden
mesh_terms: D005622:Friend murine leukemia virus / Q000235:genetics* / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D015686:Gene Products, env / Q000235:genetics* / Q000378:metabolism; D026723:Polyadenylation; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: D015686:Gene Products, env; D012333:RNA, Messenger
keywords: env mRNA; murine leukemia virus; poly(A) tail; splicing