title: A highly sensitive NADH sensor based on a mycelium-like nanocomposite using graphene oxide and multi-walled carbon nanotubes to co-immobilize poly(luminol) and poly(neutral red) hybrid films.
abstract: Hybridization of poly(luminol) (PLM) and poly(neutral red) (PNR) has been successfully performed and further enhanced by a conductive and steric hybrid nanotemplate using graphene oxide (GO) and multi-walled carbon nanotubes (MWCNTs). The morphology of the PLM-PNR-MWCNT-GO mycelium-like nanocomposite is studied by SEM and AFM and it is found to be electroactive, pH-dependent, and stable in the electrochemical system. It shows electrocatalytic activity towards NADH with a high current response and low overpotential. Using amperometry, it has been shown to have a high sensitivity of 288.9 μA mM(-1) cm(-2) to NADH (Eapp. = +0.1 V). Linearity is estimated in a concentration range of 1.33 × 10(-8) to 1.95 × 10(-4) M with a detection limit of 1.33 × 10(-8) M (S/N = 3). Particularly, it also shows another linear range of 2.08 × 10(-4) to 5.81 × 10(-4) M with a sensitivity of 151.3 μA mM(-1) cm(-2). The hybridization and activity of PLM and PNR can be effectively enhanced by MWCNTs and GO, resulting in an active hybrid nanocomposite for determination of NADH.
authors.affiliation: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, no.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan. smchen78@ms15.hinet.net.
authors.forename: Kuo
authors.initials: K
authors.lastname: Chiang Lin
authors.forename: Szu
authors.initials: S
authors.lastname: Yu Lai
authors.forename: Shen
authors.initials: S
authors.lastname: Ming Chen
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D008165:Luminol / Q000737:chemistry*; D009243:NAD / Q000032:analysis*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D009499:Neutral Red / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
chemical_list: D037742:Nanotubes, Carbon; D010087:Oxides; D011108:Polymers; D009243:NAD; D009499:Neutral Red; D008165:Luminol; D006108:Graphite