title: Development of a Norovirus P particle platform for eliciting neutralizing antibody responses to the membrane proximal external region of human immunodeficiency virus type 1 envelope.
abstract: Eliciting efficient broadly neutralizing antibodies (BnAbs) is an important goal that has yet to be achieved for human immunodeficiency type 1 (HIV-1) vaccine development, although they are rarely produced in virus-infected individuals. In particular, inducing specific neutralizing antibodies to the gp41 membrane proximal external region (MPER) has proven a difficult task. In this study, we introduce Norovirus P particles as a new platform to display the MPER epitope of HIV-1 as a vaccine with the aim of enhancing immune responses. The results showed that HIV-1 chimeric P particles were capable of inducing MPER-specific antibody responses in immunized guinea pigs, although only weakly neutralizing activity could be detected. These findings are consistent with other previous studies which have also focused on the well-studied 2F5 and 4E10 BnAbs. Our findings provide an alternate strategy for design of vaccines against HIV-1. However, great challenges remain in the effort to develop vaccines that can induce efficient HIV-1 neutralizing antibodies.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zang
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Bi
authors.forename: Dongchuan
authors.initials: D
authors.lastname: Du
authors.forename: Xintao
authors.initials: X
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Weiheng
authors.initials: W
authors.lastname: Su
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Wu
authors.forename: Yaming
authors.initials: Y
authors.lastname: Shan
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.forename: Aidong
authors.initials: A
authors.lastname: Qiu
authors.affiliation: National Engineering Laboratory of AIDS Vaccine, School of Life Sciences, Jilin University, 2699 Qianjin Street, Changchun, 130021, China. jiangcl@jlu.edu.cn.
authors.forename: Chunlai
authors.initials: C
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D006168:Guinea Pigs; D015700:HIV Envelope Protein gp41 / Q000737:chemistry* / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008969:Molecular Sequence Data; D029322:Norovirus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015700:HIV Envelope Protein gp41; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C514787:gp41 protein, Human immunodeficiency virus 1