title: The role of endothelial nitric oxide synthase gene G894T and intron 4 VNTR polymorphisms in hemodialysis patients with vascular access thrombosis.
abstract: OBJECTIVE
  Endothelial nitric oxide synthase (eNOS) gene is a candidate gene in cardiovascular and renal diseases. Several polymorphic variations have been identified in eNOS gene. We investigated a potential role of arteriovenous fistula (AVF) thrombosis and intron 4 and G894T polymorphisms in chronic renal failure.
  
  
  METHODS
  We performed a case-control observational study involving 79 with/without AVF thrombosis in chronic renal failure patients. All subjects were genotyped by the polymerase chain reaction (PCR) and PCR-Restriction Fragment Length Polymorphism. Genotype distribution and allele frequencies were compared between groups using the chi-square test.
  
  
  RESULTS
  Genotype frequencies in patients with thrombosis were not significantly different from those of patients without thrombosis for eNOS G894T polymorphism (p=0.1). eNOS gene intron 4 a allele distributions seems to be associated with thrombosis in the groups.
  
  
  CONCLUSIONS
  This study revealed that there was an association between eNOS intron 4 polymorphism and thrombosis in chronic renal failure patients. This data will be helpful in planning further eNOS association studies in vascular access thrombosis.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Erciyes University; Kayseri-Turkey. efefunda@yahoo.com.
authors.forename: Elif Funda
authors.initials: EF
authors.lastname: Şener
authors.forename: Ömer Naci
authors.initials: ÖN
authors.lastname: Emiroğulları
authors.forename: Faruk
authors.initials: F
authors.lastname: Serhatlıoğlu
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Özkul
mesh_terms: D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007438:Introns / Q000235:genetics*; D007676:Kidney Failure, Chronic / Q000097:blood / Q000235:genetics* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D006435:Renal Dialysis / Q000009:adverse effects; D013927:Thrombosis / Q000150:complications*; D014421:Turkey; D062666:Vascular Access Devices; D044465:White People
chemical_list: D002784:Cholesterol; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III