title: Cefuroxime axetil loaded solid lipid nanoparticles for enhanced activity against S. aureus biofilm.
abstract: The present research work is focused on the development of solid lipid nanoparticles of cefuroxime axetil (CA-SLN) for its enhanced inhibitory activity against Staphylococcus aureus produced biofilm. CA-SLN was prepared by solvent emulsification/evaporation method using single lipid (stearic acid (SA)) and binary lipids (SA and tristearin (TS)). Process variables such as volume of dispersion medium, concentration of surfactant, homogenization speed and time were optimized. The prepared SLN were characterized for encapsulation efficiency, drug polymer interaction studies (DSC and FT-IR), shape and surface morphology (SEM and AFM), in vitro drug release, stability studies and in vitro anti biofilm activity against S. aureus biofilm. Among the process variables, increased volume of dispersion medium, homogenization speed and time led to increase in particle size whereas increase in surfactant concentration decreased the particle size. SLN prepared using binary lipids exhibited higher entrapment efficiency than the single lipid. DSC and FT-IR studies showed no incompatible interaction between drug and excipients. CA-SLN showed two folds higher anti-biofilm activity in vitro than pristine CA against S. aureus biofilm.
authors.affiliation: Department of Pharmaceutics, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India.
authors.forename: Bhupender
authors.initials: B
authors.lastname: Singh
authors.affiliation: Department of Pharmaceutics, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India.
authors.forename: Parameswara Rao
authors.initials: PR
authors.lastname: Vuddanda
authors.affiliation: Department of Pharmaceutics, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India.
authors.forename: Vijayakumar
authors.initials: V
authors.lastname: M R
authors.affiliation: Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi 221 005, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi 221 005, India.
authors.forename: Preeti S
authors.initials: PS
authors.lastname: Saxena
authors.affiliation: Department of Pharmaceutics, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India. Electronic address: drsanjaysingh@rediffmail.com.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Singh
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D002152:Calorimetry, Differential Scanning; D002444:Cefuroxime / Q000031:analogs & derivatives* / Q000494:pharmacology; D004355:Drug Stability; D008055:Lipids / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D055672:Static Electricity
chemical_list: D008055:Lipids; D002444:Cefuroxime; C040738:cefuroxime axetil
keywords: Binary lipids; Biofilms; Cefuroxime axetil; Microbial drug resistance; Nanoparticles