title: Impedimetric detection of mutant p53 biomarker-driven metastatic breast cancers under hyposmotic pressure.
abstract: In cancer cells, the oncogenic mutant p53 (mtp53) protein is present at high levels and gain-of-function (GOF) activities with more expression of mtp53 proteins contribute to tumor growth and metastasis. Robust analytical approaches that probe the degree of metastasis of cancer cells in connection with the mtp53 activity will be extremely useful not only for establishing a better cancer prognosis but also understanding the fundamental mechanism of mtp53 oncogenic action. Here we assessed the influence of mtp53 in breast cancers to the mechanical property of breast cancer cells. Recently, ovarian and kidney cancer cell lines have been shown to have higher cellular elasticity as compared to normal cells assessed by monitoring the degree of deformation under hyposmotic pressure. To make fast detection in large scale, the impedance measurement was applied to monitor the swelling ratio of cells with time. The results showed that knockdown of mtp53 leads to decrease in cell swelling. In addition, by means of two types of impedimetric detection systems we consistently detected enhancement of impedance signal in mtp53-expressing breast cancer cells. Based on this observation we hypothesize that highly expressed mtp53 in metastatic mutant breast cancers can promote tumor progression by making cells more deformable and easier to spread out through extracellular matrix. The identification via the electric measurement can be accomplished within 10 minutes. All results in this report suggest that electric probing for the extent of the mtp53 expression of breast cancer cells may serve as a meaningful fingerprint for the cancer diagnostics, and this outcome will also have an important clinical implication for the development of mtp53-based targeting for tumor detection and treatment.
authors.affiliation: Department of Chemistry, Hunter College and the Graduate Center, City University of New York, New York, New York, United States of America.
authors.forename: Menglu
authors.initials: M
authors.lastname: Shi
authors.affiliation: Department of Biological Sciences Hunter College and the Graduate Center, City University of New York, New York, New York, United States of America.
authors.forename: Nataly
authors.initials: N
authors.lastname: Shtraizent
authors.affiliation: Department of Biological Sciences Hunter College and the Graduate Center, City University of New York, New York, New York, United States of America.
authors.forename: Alla
authors.initials: A
authors.lastname: Polotskaia
authors.affiliation: Department of Biological Sciences Hunter College and the Graduate Center, City University of New York, New York, New York, United States of America.
authors.forename: Jill
authors.initials: J
authors.lastname: Bargonetti
authors.affiliation: Department of Chemistry, Hunter College and the Graduate Center, City University of New York, New York, New York, United States of America.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsui
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D001696:Biomechanical Phenomena; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000502:physiology; D048429:Cell Size; D004548:Elasticity; D005260:Female; D006801:Humans; D009362:Neoplasm Metastasis; D009997:Osmotic Pressure; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53