title: Effects of gelsolin on macrophage inflammatory responses to orthopaedic implant wear debris.
abstract: The local effects of implant wear debris on surrounding tissue has been a major focus of many investigators. Although there have been improvements in implants, significant numbers of revision surgeries are performed to address these issues. Gelsolin (GSN) is a protein in the cytoplasm and circulating serum involved in actin breakdown as well as anti-inflammatory processes. In this study, we tested the hypothesis that GSN in the presence of wear debris in vitro decreases the inflammatory response of a human monocyte cell line. We utilized titanium-, polyethylene-, and cobalt-characterized wear particles in a 1:100 and a 1:500 cell-to-particle ratios in the presence of a low (0.2 µM) and normal (2.0 µM) concentrations of GSN and compared the inflammatory response to cells without GSN exposure. The results show that IL-6, IL-1, TNF-α, and PGE₂ all increased with higher concentrations of GSN. Although the anti-inflammatory properties of GSN were not seen in this in vitro experiment, it has previously been shown that GSN does affect the inflammatory response of monocytes to orthopedic implant wear debris. The dose-response curve for GSN may have a bimodal profile, which should be further investigated.
authors.affiliation: Campbell Clinic Department of Orthopaedics & Biomedical Engineering University of Tennessee Health Science Center, TN, USA.
authors.forename: William M
authors.initials: WM
authors.lastname: Mihalko
authors.affiliation: Campbell Clinic Department of Orthopaedics and Biomedical Engineering, University of Tennessee Health Science Center, Memphis, TN.
authors.forename: Lev
authors.initials: L
authors.lastname: Djenderedjian
authors.affiliation: Campbell Clinic Department of Orthopaedics and Biomedical Engineering, University of Tennessee Health Science Center, Memphis, TN.
authors.forename: Paramjeet S
authors.initials: PS
authors.lastname: Cheema
authors.affiliation: UTHSC.
authors.forename: Richard
authors.initials: R
authors.lastname: Smith
mesh_terms: D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism; D018260:Gelsolin / Q000494:pharmacology*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism*; D020959:Polyethylene / Q000494:pharmacology; D014025:Titanium / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018260:Gelsolin; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D003035:Cobalt; D020959:Polyethylene; D014025:Titanium; D015232:Dinoprostone