title: Sorting signal targeting mRNA into hepatic extracellular vesicles.
abstract: Intercellular communication mediated by extracellular vesicles has proved to play an important role in normal and pathological scenarios. However not too much information about the sorting mechanisms involved in loading the vesicles is available. Recently, our group has characterized the mRNA content of vesicles released by hepatic cellular systems, showing that a set of transcripts was particularly enriched in the vesicles in comparison with their intracellular abundance. In the current work, based on in silico bioinformatics tools, we have mapped a novel sequence of 12 nucleotides C[TA]G[GC][AGT]G[CT]C[AT]GG[GA], which is significantly enriched in the set of mRNAs that accumulate in extracellular vesicles. By including a 3'-UTR containing this sequence in a luciferase mRNA reporter, we have shown that in a hepatic cellular system this reporter mRNA was incorporated into extracellular vesicles. This study identifies a sorting signal in mRNAs that is involved in their enrichment in EVs, within a hepatic non-tumoral cellular model.
authors.affiliation: Institute of Computing Science; Poznan University of Technology; Poznan, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Szostak
authors.affiliation: Metabolomics Unit; CIC bioGUNE; CIBERehd; Bizkaia Technology Park; Derio, Bizkaia Spain.
authors.forename: Felix
authors.initials: F
authors.lastname: Royo
authors.affiliation: Institute of Computing Science; Poznan University of Technology; Poznan, Poland; Institute of Bioorganic Chemistry; Polish Academy of Sciences; Poznan, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Rybarczyk
authors.affiliation: Institute of Computing Science; Poznan University of Technology; Poznan, Poland; Institute of Bioorganic Chemistry; Polish Academy of Sciences; Poznan, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Szachniuk
authors.affiliation: Institute of Computing Science; Poznan University of Technology; Poznan, Poland; Institute of Bioorganic Chemistry; Polish Academy of Sciences; Poznan, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Blazewicz
authors.affiliation: Luxembourg Centre for Systems Biomedicine (LCSB); University of Luxembourg; Luxembourg.
authors.forename: Antonio
authors.initials: A
authors.lastname: del Sol
authors.affiliation: Metabolomics Unit; CIC bioGUNE; CIBERehd; Bizkaia Technology Park; Derio, Bizkaia Spain; IKERBASQUE; Basque Foundation for Science; Bilbao, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Falcon-Perez
mesh_terms: D020413:3' Untranslated Regions; D002450:Cell Communication; D002460:Cell Line; D019295:Computational Biology / Q000379:methods*; D020869:Gene Expression Profiling; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D059372:Nucleotide Motifs; D034443:RNA Transport; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D022161:Transport Vesicles
chemical_list: D020413:3' Untranslated Regions; D012333:RNA, Messenger
keywords: extracellular vesicles; mRNA; sorting signal