title: Pro-healing effects of bilirubin in open excision wound model in rats.
abstract: Bilirubin, a by-product of heme degradation, has an important role in cellular protection. Therefore, we speculated that bilirubin could be of potential therapeutic value in wound healing. To validate the hypothesis, we used a full-thickness cutaneous wound model in rats. Bilirubin (30 mg/kg) was administered intraperitoneally every day for 9 days. The surface area of the wound was measured on days 0, 2, 4, 7 and 10 after the creation of the wound. The granulation tissue was collected on day 10 post-wounding for analysing various parameters of wound healing. Bilirubin treatment accelerated wound contraction and increased hydroxyproline and glucosamine contents. mRNA expression of pro-inflammatory factors such as intercellular cell adhesion molecule-1 (ICAM-1) and tumour necrosis factor-α (TNF-α) were down-regulated and that of anti-inflammatory cytokine interleukin-10 (IL-10) was up-regulated. The findings suggest that bilirubin could be a new agent for enhancing cutaneous wound healing.
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Bareilly, India.
authors.forename: Azad A
authors.initials: AA
authors.lastname: Ahanger
authors.affiliation: Department of Physiology, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: Marie D
authors.initials: MD
authors.lastname: Leo
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Bareilly, India.
authors.forename: Anu
authors.initials: A
authors.lastname: Gopal
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Bareilly, India.
authors.forename: Vinay
authors.initials: V
authors.lastname: Kant
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Bareilly, India.
authors.forename: Surendra K
authors.initials: SK
authors.lastname: Tandan
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Bareilly, India.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001663:Bilirubin; D016753:Interleukin-10; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha; D014945:Wound Healing
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D001663:Bilirubin
keywords: Bilirubin; Excision wound; Healing; ICAM-1; IL-10; TNF-α; mRNA expression