title: Pyrene chromophores for the photoreversal of psoralen interstrand crosslinks.
abstract: Applying psoralen interstrand crosslinks for the photoactivation of nucleic acids is a new concept. To find chromophores that can efficiently stimulate crosslink repair we screened several pyrenes and appended them to peptide nucleic acids for their site-selective addressing. Even though pyrenes conjugated to uracil revealed desirable spectroscopic properties they were not effective in crosslink reversal. In contrast, bare pyrenes are well suitable for crosslink repair with 350 nm light showing an uncaging efficiency similar to classical photocaging groups.
authors.affiliation: Interfaculty Institute of Biochemistry, Auf der Morgenstelle 15. and University of Tübingen, 72076 Tübingen, Germany. thorsten.stafforst@uni-tuebingen.de.
authors.forename: Jens M
authors.initials: JM
authors.lastname: Stadler
authors.forename: Thorsten
authors.initials: T
authors.lastname: Stafforst
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D004260:DNA Repair; D005363:Ficusin / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D011721:Pyrenes / Q000138:chemical synthesis* / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D020135:Peptide Nucleic Acids; D011721:Pyrenes; D004247:DNA; C030984:pyrene; D005363:Ficusin