title: Gastric cancer-derived MSC-secreted PDGF-DD promotes gastric cancer progression.
abstract: OBJECTIVE
  This study was designed to investigate the role of PDGF-DD secreted by gastric cancer-derived mesenchymal stem cells (GC-MSCs) in human gastric cancer progression.
  
  
  METHODS
  Gastric cancer cells were indirectly co-cultured with GC-MSCs in a transwell system. The growth and migration of gastric cancer cells were evaluated by cell colony formation assay and transwell migration assay, respectively. The production of PDGF-DD in GC-MSCs was determined by using Luminex and ELISA. Neutralization of PDGFR-β by su16f and siRNA interference of PDGF-DD in GC-MSCs was used to demonstrate the role of PDGF-DD produced by GC-MSCs in gastric cancer progression.
  
  
  RESULTS
  GC-MSC conditioned medium promoted gastric cancer cell proliferation and migration in vitro and in vivo. Co-culture with GC-MSCs increased the phosphorylation of PDGFR-β in SGC-7901 cells. Neutralization of PDGFR-β by su16f blocked the promoting role of GC-MSC conditioned medium in gastric cancer cell proliferation and migration. Recombinant PDGF-DD duplicated the effects of GC-MSC conditioned medium on gastric cancer cells. Knockdown of PDGF-DD in GC-MSCs abolished its effects on gastric cancer cells in vitro and in vivo.
  
  
  CONCLUSIONS
  PDGF-DD secreted by GC-MSCs is capable of promoting gastric cancer cell progression in vitro and in vivo. Targeting the PDGF-DD/PDGFR-β interaction between MSCs and gastric cancer cells may represent a novel strategy for gastric cancer therapy.
authors.affiliation: Centre for Clinical Laboratory of the Affiliated Hospital, Key Laboratory of Laboratory Medicine of Jiangsu Province, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 212001, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Cai
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zixuan
authors.initials: Z
authors.lastname: Sun
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Wu
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Hui
authors.initials: H
authors.lastname: Qian
authors.forename: Wenrong
authors.initials: W
authors.lastname: Xu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018920:Coculture Techniques; D018450:Disease Progression; D006801:Humans; D008222:Lymphokines / Q000378:metabolism* / Q000502:physiology; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000378:metabolism* / Q000502:physiology; D011499:Protein Processing, Post-Translational; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D047368:Tumor Burden
chemical_list: D008222:Lymphokines; C427493:PDGFD protein, human; D010982:Platelet-Derived Growth Factor; D020797:Receptor, Platelet-Derived Growth Factor beta