title: Molecular and Serological Intraocular Fluid Analysis of Coxiella burnetii-seropositive Patients with Concurrent Idiopathic Uveitis.
abstract: OBJECTIVE
  Previous studies have suggested a link between Q fever and uveitis. We determined whether Coxiella burnetii causes intraocular infection in C. burnetii-seropositive patients with idiopathic uveitis.
  
  
  METHODS
  From a retrospective observational case series, paired aqueous humor and serum samples from 10 C. burnetii-seropositive patients with idiopathic uveitis were examined for intraocular antibody production by using the Goldmann-Witmer coefficient and by polymerase chain reaction (PCR).
  
  
  RESULTS
  Although intraocular IgG against C. burnetii was detected, no intraocular antibody production was observed (low Goldmann Wittmer coefficients). All PCR results were negative.
  
  
  CONCLUSIONS
  Uveitis due to an intraocular infection with C. burnetii is unlikely.
authors.affiliation: a Department of Virology .|b Department of Ophthalmology .
authors.forename: L E
authors.initials: LE
authors.lastname: Hermans
authors.affiliation: c Department of Internal Medicine and Infectious Diseases .
authors.forename: J J
authors.initials: JJ
authors.lastname: Oosterheert
authors.affiliation: c Department of Internal Medicine and Infectious Diseases .
authors.forename: L M
authors.initials: LM
authors.lastname: Kampschreur
authors.affiliation: b Department of Ophthalmology .
authors.forename: J
authors.initials: J
authors.lastname: Ossewaarde-van Norel
authors.affiliation: a Department of Virology .|b Department of Ophthalmology .
authors.forename: J
authors.initials: J
authors.lastname: Dekkers
authors.affiliation: b Department of Ophthalmology .|d Department of Ophthalmology , Erasmus Medical Center , Rotterdam , The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Rothova
authors.affiliation: a Department of Virology .|b Department of Ophthalmology .
authors.forename: J D F
authors.initials: JD
authors.lastname: de Groot-Mijnes
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D001082:Aqueous Humor / Q000276:immunology* / Q000382:microbiology; D016997:Coxiella burnetii / Q000235:genetics / Q000276:immunology*; D004269:DNA, Bacterial / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D015818:Eye Infections, Bacterial / Q000276:immunology / Q000382:microbiology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011778:Q Fever / Q000276:immunology / Q000382:microbiology*; D012189:Retrospective Studies; D014605:Uveitis / Q000276:immunology / Q000382:microbiology*; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D007074:Immunoglobulin G
keywords: Aqueous humor analysis; Coxiella burnetii; infection; uveitis