title: Glutamate and GABA-metabolizing enzymes in post-mortem cerebellum in Alzheimer's disease: phosphate-activated glutaminase and glutamic acid decarboxylase.
abstract: Enzymes of glutamate and GABA metabolism in postmortem cerebellum from patients with Alzheimer's disease (AD) have not been comprehensively studied. The present work reports results of original comparative study on levels of phosphate-activated glutaminase (PAG) and glutamic acid decarboxylase isoenzymes (GAD65/67) in autopsied cerebellum samples from AD patients and matched controls (13 cases in each group) as well as summarizes published evidence for altered levels of PAG and GAD65/67 in AD brain. Altered (decreased) levels of these enzymes and changes in links between amounts of these enzymes and other glutamate-metabolizing enzymes (such as glutamate dehydrogenase and glutamine synthetase-like protein) in AD cerebella suggest significantly impaired glutamate and GABA metabolism in this brain region, which was previously regarded as not substantially involved in AD pathogenesis.
authors.affiliation: Mental Health Research Center, Russian Academy of Medical Sciences, 34, Kashirskoye Shosse, Moscow, Russia, 115522.
authors.forename: G Sh
authors.initials: GSh
authors.lastname: Burbaeva
authors.forename: I S
authors.initials: IS
authors.lastname: Boksha
authors.forename: E B
authors.initials: EB
authors.lastname: Tereshkina
authors.forename: O K
authors.initials: OK
authors.lastname: Savushkina
authors.forename: T A
authors.initials: TA
authors.lastname: Prokhorova
authors.forename: E A
authors.initials: EA
authors.lastname: Vorobyeva
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology*; D019732:Amide Synthases / Q000378:metabolism; D002531:Cerebellum / Q000201:enzymology*; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D005972:Glutaminase / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D005969:Glutamate Dehydrogenase; D005972:Glutaminase; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1; C401141:glutamate decarboxylase 2; D019732:Amide Synthases; C417025:glutamine synthetase-like protein, human