title: Development of an ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry method for the simultaneous determination of salicylic acid, jasmonic acid, and abscisic acid in rose leaves.
abstract: This paper describes a method to detect and quantitate the endogenous plant hormones (±)-2-cis-4-trans-abscisic acid, (-)-jasmonic acid, and salicylic acid by means of ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) in hybrid rose leaf matrices. Deuterium-labeled [(2)H6] (+)-2-cis-4-trans-abscisic acid, [(2)H6] (±)-jasmonic acid, and [(2)H4]-salicylic acid were used as internal standards. Rose samples (10 mg) were extracted with methanol/water/acetic acid (10:89:1) and subsequently purified on an Oasis MCX 1 cm(3) Vac SPE cartridge. Performance characteristics were validated according to Commission Decision 2002/657/EC. Recovery, repeatability, and within-laboratory reproducibility were acceptable for all phytohormones tested at three different concentrations. The decision limit and detection capability for (±)-2-cis-4-trans-abscisic acid, (-)-jasmonic acid, and salicylic acid were 0.0075 and 0.015 μg/g, 0.00015 and 0.00030 μg/g, and 0.0089 and 0.018 μg/g, respectively. Matrix effects (signal suppression or enhancement) appeared to be high for all substances considered, implying the need for quantitation based on matrix-matched calibration curves.
authors.affiliation: Department of Agricultural, Forest and Food Sciences, University of Torino , Largo Paolo Braccini 2, 10095 Grugliasco, Torino, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Bosco
authors.forename: Els
authors.initials: E
authors.lastname: Daeseleire
authors.forename: Els
authors.initials: E
authors.lastname: Van Pamel
authors.forename: Valentina
authors.initials: V
authors.lastname: Scariot
authors.forename: Leen
authors.initials: L
authors.lastname: Leus
mesh_terms: D000040:Abscisic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003517:Cyclopentanes / Q000032:analysis*; D054883:Oxylipins / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D027826:Rosa / Q000737:chemistry*; D020156:Salicylic Acid / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010936:Plant Extracts; C011006:jasmonic acid; D000040:Abscisic Acid; D020156:Salicylic Acid