title: Maldi-tof fingerprinting of seminal plasma lipids in the study of human male infertility.
abstract: This study proposed lipid fingerprinting of human seminal plasma by mass spectrometry as an analytical method to differentiate biological conditions. For this purpose, we chose infertile men as a model to study specific conditions, namely: high and low seminal plasma lipid peroxidation levels (sub-study 1.1), high and low sperm nuclear DNA fragmentation (sub-study 1.2), and intervention status: before and after subinguinal microsurgical varicocelectomy (study 2). Study 1 included 133 patients, of which 113 were utilized for sub-study 1.1 and 89 for sub-study 1.2. Study 2 included 17 adult men submitted to subinguinal varicocelectomy, before and 90 days after varicocelectomy. Lipids were extracted from seminal plasma and submitted to Matrix-Assisted Laser Desorption Ionization Quadrupole-Time-of-Flight Mass Spectrometry in the positive ionization mode. Spectra were processed using Waters(®) MassLynx, and MetaboAnalyst online software was used for statistical analyses. For sub-studies 1.1 and 1.2, and study 2, univariate analysis revealed 8, 87 and 34 significant ions, respectively. Multivariate analysis was performed through PCA and PLS-DA. PCA generated 56, 32 and 34 components respectively for each study and these were submitted to logistic regression. A ROC curve was plotted and the area under the curve was equal to 97.4, 92.5 and 96.5%. PLS-DA generated a list of 19, 24 and 23 VIP ions for sub-studies 1.1 and 1.2, and study 2, respectively. Therefore, this study established the lipid profile and comparison of patterns altered in response to specific biological conditions.
authors.affiliation: Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University, R Embau, 231, Sao Paulo, 04039-060, SP, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Camargo
authors.forename: Paula
authors.initials: P
authors.lastname: Intasqui
authors.forename: Camila Bruna
authors.initials: CB
authors.lastname: de Lima
authors.forename: Daniela Antunes
authors.initials: DA
authors.lastname: Montani
authors.forename: Marcílio
authors.initials: M
authors.lastname: Nichi
authors.forename: Eduardo Jorge
authors.initials: EJ
authors.lastname: Pilau
authors.forename: Fabio Cesar
authors.initials: FC
authors.lastname: Gozzo
authors.forename: Edson Guimarães
authors.initials: EG
authors.lastname: Lo Turco
authors.forename: Ricardo Pimenta
authors.initials: RP
authors.lastname: Bertolla
mesh_terms: D000328:Adult; D020552:Comet Assay; D003430:Cross-Sectional Studies; D053938:DNA Fragmentation; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism*; D015227:Lipid Peroxidation; D008055:Lipids / Q000032:analysis*; D008297:Male; D015999:Multivariate Analysis; D018384:Oxidative Stress; D011446:Prospective Studies; D012372:ROC Curve; D012661:Semen / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013081:Sperm Motility; D014646:Varicocele / Q000378:metabolism / Q000601:surgery
chemical_list: D008055:Lipids