title: Antitumoural activity of viniferin-enriched extracts from Vitis vinifera L. cell cultures.
abstract: The aim of this work was to evaluate the effect of stilbenes from different cultivars of Vitis vinifera on tumour proliferation. Extracts were obtained from elicited V. vinifera cell cultures and characterised by HPLC/DAD/MS. Cell growth was evaluated in four human cancer cell lines and in normal human fibroblasts. The cells were exposed to the extracts or to trans-resveratrol, used as reference molecule, for 48 h, at 1-10 μM concentrations of total stilbenoids. All the extracts exhibited antiproliferative activity, mediated by modulation of the cell cycle and induction of cytotoxicity in cancer but not in normal cell lines, and positively correlated with the content in dimeric stilbenoids. The Alphonse Lavallée extract was the most active, and the obtained stilbenoid fraction resulted 8-10 times more active than trans-resveratrol. Extracts from V. vinifera cell cultures could represent new sources of active stilbenoid compounds to be further assayed in in vivo studies for their antitumoural properties.
authors.affiliation: a Division Pharmacology and Toxicology, Department of NEUROFARBA , V. le G. Pieraccini, 6, 50139 Florence , Italy.
authors.forename: L
authors.initials: L
authors.lastname: Giovannelli
authors.forename: M
authors.initials: M
authors.lastname: Innocenti
authors.forename: A R
authors.initials: AR
authors.lastname: Santamaria
authors.forename: E
authors.initials: E
authors.lastname: Bigagli
authors.forename: G
authors.initials: G
authors.lastname: Pasqua
authors.forename: N
authors.initials: N
authors.lastname: Mulinacci
mesh_terms: D000972:Antineoplastic Agents, Phytogenic; D001572:Benzofurans / Q000302:isolation & purification* / Q000494:pharmacology*; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry*; D000077185:Resveratrol; D013237:Stereoisomerism; D013267:Stilbenes / Q000032:analysis / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry* / Q000235:genetics
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001572:Benzofurans; D010936:Plant Extracts; D013267:Stilbenes; C064176:alpha-viniferin; D000077185:Resveratrol
keywords: breast cancer cell lines; hepatocellular carcinoma cells; stilbenes; trans-resveratrol; viniferins