title: Direct electrochemical and AFM detection of amyloid-β peptide aggregation on basal plane HOPG.
abstract: Amyloidogenesis is associated with more than 30 human diseases, including Alzheimer's which is related to aggregation of β-amyloid peptide (Aβ). Here, consecutive stages of Aβ42 aggregation and amyloid fibril formation were followed electrochemically via oxidation of tyrosines in Aβ42 adsorbed on the basal plane graphite electrode and directly correlated with Aβ42 morphological changes observed by atomic force microscopy of the same substrate. The results offer new tools for analysis of mechanisms of Aβ aggregation.
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Science and Technology, Aarhus University, Gustav Wieds Vej 1590-14, DK-8000, Aarhus C, Denmark. elena.ferapontova@inano.au.dk.
authors.forename: Paula
authors.initials: P
authors.lastname: Lopes
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.forename: Yanlian
authors.initials: Y
authors.lastname: Yang
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Elena E
authors.initials: EE
authors.lastname: Ferapontova
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000378:metabolism; D055664:Electrochemical Techniques; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D018625:Microscopy, Atomic Force; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000032:analysis* / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D014443:Tyrosine; D006108:Graphite