title: Proteomic identification of pterostilbene-mediated anticancer activities in HepG2 cells.
abstract: In the present study, we attempt to shed light on the underlying molecular mechanism of the anticancer activity of pterostilbene (PTS) in HepG2 cells through the proteomic approach. PTS was found to induce apoptosis by altering the expression of apoptotic genes and the G2/M phase of cell cycle arrest. Further, the 2-DE map showed the expression of 72 differentially regulated proteins in PTS-treated HepG2 cells, of which 8 spots with >2 fold up- or down-regulated level were identified by MALDI-TOF analysis, which has a regulatory role in apoptosis. These findings for the first time offer valuable insights into the mechanism of apoptotis by PTS in HepG2 cells.
authors.affiliation: SRM Research Institute, SRM University , Kattankulathur, Tamilnadu, India.
authors.forename: N
authors.initials: N
authors.lastname: Suganya
authors.forename: E
authors.initials: E
authors.lastname: Bhakkiyalakshmi
authors.forename: T S
authors.initials: TS
authors.lastname: Subin
authors.forename: K
authors.initials: K
authors.lastname: Krishnamurthi
authors.forename: S Saravana
authors.initials: SS
authors.lastname: Devi
authors.forename: K
authors.initials: K
authors.lastname: Lau
authors.forename: T V
authors.initials: TV
authors.lastname: Sekar
authors.forename: R
authors.initials: R
authors.lastname: Paulmurugan
authors.forename: K M
authors.initials: KM
authors.lastname: Ramkumar
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D056945:Hep G2 Cells; D022781:Hepatocytes; D006801:Humans; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013267:Stilbenes / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry
chemical_list: D000970:Antineoplastic Agents; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D013267:Stilbenes; C107773:pterostilbene