title: Effects of anionic surfactant on extraction of free fatty acid from Chlorella vulgaris.
abstract: Microalgal lipid with a high free fatty acid (FFA) content was directly extracted from Chlorella vulgaris, using SDBS, in an acid-catalyzed hot-water extraction process. The total fatty acid content of C. vulgaris was 296.0 mg/g cell. Under the 1.0% sulfuric acid, 0.4% SDBS conditions, the FFA content of the lipid increased to 96.7%, and the lipid-extraction yield was 248.4 mg/g cell. Under the 2.0% sulfuric acid, 0.2% SDBS conditions, the FFA content of the lipid was 96.1%, and the lipid-extraction yield was 266.0mg/g cell. Whereas the FAME content of the microalgal lipid extracted by hexane-methanol was 76.4% at the 10.0% sulfuric acid concentration, the FAME content of the high-FFA microalgal lipid was increased to 70.1% at a sulfuric acid concentration of only 0.1%. By combined sulfuric acid/SDBS treatment, high-FFA microalgal lipid was extracted in large yields; moreover, the amount of catalyst was remarkably reduced in the esterification of FFA.
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea. Electronic address: yearn@kier.re.kr.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Park
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: Bora
authors.initials: B
authors.lastname: Nam
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Choi
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: You-Kwan
authors.initials: YK
authors.lastname: Oh
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: Jin-Suk
authors.initials: JS
authors.lastname: Lee
mesh_terms: D056804:Biofuels; D002384:Catalysis; D048408:Chlorella vulgaris / Q000737:chemistry*; D005230:Fatty Acids, Nonesterified / Q000302:isolation & purification*; D008055:Lipids / Q000737:chemistry; D058086:Microalgae / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D056804:Biofuels; D005230:Fatty Acids, Nonesterified; D008055:Lipids; D013501:Surface-Active Agents
keywords: Biodiesel; Chlorella vulgaris; Esterification; Free fatty acid extraction; Sodium dodecyl benzene sulfonate