title: Wightianines A-E, dihydro-β-agarofuran sesquiterpenes from Parnassia wightiana, and their antifungal and insecticidal activities.
abstract: Five new sesquiterpene polyol esters with a dihydro-β-agarofuran skeleton, designated as wightianines A-E (1-5), besides two known compounds, were isolated from the methanolic extract of the whole plant of the traditional herbal medicine Parnassia wightiana Wall. The structures of the isolated compounds were elucidated on the basis of spectroscopic analyses, including two-dimensional nuclear magnetic resonance techniques (correlation spectroscopy, heteronuclear multiple-quantum coherence, nuclear Overhauser effect spectrometry, and heteronuclear multiple-bond correlation) and electronic circular dichroism studies. The antifungal and insecticidal activities of five compounds were evaluated against several plant pathogenic fungi and armyworm larvae (Mythimna separata Walker). Among the test metabolites, compounds 2 and 7 both exhibited potent antifungal activity against the phytopathogenic fungus Cytospora sp. with minimum inhibitory concentration values of 0.78 μg/mL, which are equal to the two positive controls, hymexazol and carbendazim. However, no insecticidal activity of the test compounds was observed in the present study. Compounds 2 and 7 could be promising leads for developing new fungicides against agriculturally important fungus Cytospora sp.
authors.affiliation: College of Forestry, and §Shaanxi Engineering Center of Bioresource Chemistry and Sustainable Utilization, College of Science, Northwest A&F University , Yangling, Shaanxi 712100, People's Republic of China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Wang
authors.forename: Cheng-Chen
authors.initials: CC
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Nusrat
authors.initials: N
authors.lastname: Shafiq
authors.forename: Gennaro
authors.initials: G
authors.lastname: Pescitelli
authors.forename: Deng-Wu
authors.initials: DW
authors.lastname: Li
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Gao
mesh_terms: D000818:Animals; D029753:Celastraceae / Q000737:chemistry*; D002942:Circular Dichroism; D047309:Flavones / Q000737:chemistry / Q000494:pharmacology*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects; D007306:Insecticides; D007814:Larva; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D009036:Moths; D010936:Plant Extracts / Q000737:chemistry; D004718:Saccharomycetales / Q000187:drug effects; D012717:Sesquiterpenes / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D047309:Flavones; D005659:Fungicides, Industrial; D007306:Insecticides; D010936:Plant Extracts; D012717:Sesquiterpenes; C498950:wightianin; C079978:dihydroagarofuran