title: Expression pattern of heme oxygenase 1 gene and hypoxic adaptation in chicken embryos.
abstract: Heme oxygenase 1 (HO-1), a rate-limiting enzyme of heme catabolism, has a crucial role of cytoprotective functions under hypoxia. The objective of the present study was to investigate potential differences in protective effect of HO-1 gene on chicken (Gallus gallus) embryo lung during late incubation. At embryonic day (D) D16, D18, D19, and D20 of incubation, the expression of HO-1 in the lungs of chicken embryos (Tibet and Shouguang chickens) incubated in normoxic (21% O2) and hypoxic (13% O2) conditions was measured. SNPs were screened within 5'-flanking region and coding regions with PCR-sequencing and the genotype of the SNPs was determined with PCR-RFLP in Tibet, Chahua and Shouguang chicken populations. In conclusion, the Tibet chicken had higher HO-1 expression on D19 under hypoxic incubation and had two SNPs with different frequency distributions from other chicken breeds, which might be a way that the Tibet chicken had hereditary adaptation to hypoxia during embryonic development.
authors.affiliation: National Engineering Laboratory For Animal Breeding, China Agricultural University, Beijing 100193, China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Gou
authors.affiliation: National Engineering Laboratory For Animal Breeding, China Agricultural University, Beijing 100193, China.
authors.forename: Junfei
authors.initials: J
authors.lastname: Peng
authors.affiliation: School of Biological Science and Medical Engineering, Beijing University of Aeronautics & Astronautics, Beijing 100191, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.affiliation: National Engineering Laboratory For Animal Breeding, China Agricultural University, Beijing 100193, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory For Animal Breeding, China Agricultural University, Beijing 100193, China. Electronic address: zhanghao827@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory For Animal Breeding, China Agricultural University, Beijing 100193, China.
authors.forename: Changxin
authors.initials: C
authors.lastname: Wu
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D002642:Chick Embryo; D005838:Genotype; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D000860:Hypoxia / Q000196:embryology / Q000378:metabolism*; D008168:Lung / Q000196:embryology / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide
chemical_list: D051547:Heme Oxygenase-1
keywords: Expression pattern; HO-1; Hypoxic adaptation; Tibet chicken