title: [Activity of glial cells in trigeminal nervous system in rats with experimental pulpitis].
abstract: OBJECTIVE
  To observe the activity change of astrocyte in related nucleus caused by acute pulpitis in rats.
  
  
  METHODS
  Rat acute pulpitis model was induced by lipopolysaccharides (LPS). And, according to processing time, a total of 30 rats were divided into 5 groups of control, 6, 12, 24 and 48 h. Immunohistochemistry and Western blot were employed to detect the dynamic expression of glial fibrillary acidic protein (GFAP) in spinal nucleus of trigeminal nerve (Vc).
  
  
  RESULTS
  The relative gray value of ipsilateral Vc GFAP expression in experimental groups was 153 ± 11 at 12 h. And it significantly increased versus the control group (100 ± 4)(P < 0.05). The relative gray value of GFAP was 198 ± 12 at 24 h and was 231 ± 9 at 48 h. And they significantly increased versus the control group (both P < 0.01). Their expressions were time-dependent.
  
  
  CONCLUSIONS
  In this acute pulpitis model, activated glial cells are probably involved in the processes of pulpitis and hyperalgesia.
authors.affiliation: Department of Stomatology, Chinese PLA General Hospital & Postgraduate Military Medical School, Beijing 100853, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Gu
authors.affiliation: Department of Stomatology, Chinese PLA General Hospital & Postgraduate Military Medical School, Beijing 100853, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Stomatology, Chinese PLA General Hospital & Postgraduate Military Medical School, Beijing 100853, China. Email: liuhcpla@gmail.com.
authors.forename: Hongchen
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000473:pathology*; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006930:Hyperalgesia / Q000139:chemically induced / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D011671:Pulpitis / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014276:Trigeminal Nerve / Q000473:pathology*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D008070:Lipopolysaccharides