title: First llama (Lama glama) pregnancy obtained after in vitro fertilization and in vitro culture of gametes from live animals.
abstract: The aim of this study was to evaluate the developmental competence and pregnancy rate of llama hatched blastocysts produced in vitro using gametes from live animals and two different culture conditions. Fifteen adult females were superstimulated with 1500 IU of eCG, eleven (73%) responded to the treatment and were used as oocyte donors. Follicular aspiration was conducted by flank laparotomy. Semen collections were performed under general anesthesia by electroejaculation of the male. Sixty-six COCs were recovered from 77 aspirated follicles (86% recovery) and were randomly placed in Fertil-TALP microdroplets with the sperm suspension (20 × 10(6)live spermatozoa/ml). After 24 h, they were placed in SOFaa medium supplemented with FCS and randomly assigned to one of two culture conditions. Culture condition 1 (CC1) consisted of 6 days of culture (n=28) and culture condition 2 (CC2) consisted of renewing the culture medium every 48 h (n=35). In CC1, the blastocyst rate was 36% (10/28) and the hatched blastocyst rate was 28% (8/28) whereas in CC2, the blastocyst rate was 34% (12/35) and the hatched blastocyst rate was 20% (7/35) (p>0.05). No pregnancies were obtained after embryo transfer (ET) of CC1 blastocysts (0/8) while one pregnancy was obtained (1/7) after transferring a hatched blastocyst from CC2. Forty-two days after the ET, the pregnancy was lost. This study represents the first report of a pregnancy in the llama after intrauterine transfer of embryos produced by in vitro fertilization using gametes from live animals.
authors.affiliation: Cátedra de Teriogenología Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Argentina. Electronic address: vickytrasorras@hotmail.com.
authors.forename: V
authors.initials: V
authors.lastname: Trasorras
authors.affiliation: Cátedra de Teriogenología Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Baca Castex
authors.affiliation: Cátedra de Teriogenología Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Alonso
authors.affiliation: Cátedra de Teriogenología Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Argentina; Cátedra de Física Biológica, Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires (UBA), Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Giuliano
authors.affiliation: Cátedra de Teriogenología Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Argentina.
authors.forename: R
authors.initials: R
authors.lastname: Santa Cruz
authors.affiliation: Cátedra de Teriogenología Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Arraztoa
authors.affiliation: Cátedra de Teriogenología Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Argentina.
authors.forename: G
authors.initials: G
authors.lastname: Chaves
authors.affiliation: Cátedra de Cirugía, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires (UBA), Argentina.
authors.forename: D
authors.initials: D
authors.lastname: Rodríguez
authors.affiliation: Cátedra de Teriogenología Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Argentina.
authors.forename: D
authors.initials: D
authors.lastname: Neild
authors.affiliation: Cátedra de Teriogenología Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Miragaya
mesh_terms: D000818:Animals; D002161:Camelids, New World / Q000502:physiology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D046149:Embryo Culture Techniques / Q000662:veterinary; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D008297:Male; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D012661:Semen / Q000166:cytology / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology; D020858:Tissue and Organ Harvesting / Q000379:methods / Q000662:veterinary
chemical_list: D006063:Chorionic Gonadotropin
keywords: Embryo transfer; IVC; IVF; Llama; Pregnancy; SOF