title: Involvement of RbAp48 in erythroid differentiation of murine erythroleukemia cells induced by sodium butyrate.
abstract: Normal mammalian terminal erythroid differentiation is a precisely regulated process during which the progenitor cells execute particular programs to form a mature erythrocytic phenotype. In the present study, it was found that RbAp48, a histone-binding protein associated with retinoblastoma protein, was upregulated during terminal erythroid maturationin vivoandin vitro. This indicated that RbAp48, at least in part, participated in the regulation of murine erythropoiesis. Following sodium butyrate (SB) induction, murine erythroleukemia (MEL) cells began to re-enter erythroid differentiation and the ratio of differentiated cells reached ~80% at 72 h. The erythroid maturation-related mRNA expression of α-globin, β-globin and glycophorin A (GPA) was increased markedly, which indicated that SB induced MEL differentiation. During MEL differentiation, the RbAp48 level showed a 1.5-fold increase at 72 h, and the globin transcription factor (GATA)-1 level was also upregulated in the early stage of differentiation. By contrast, the c-Myc level was gradually downregulated in MEL differentiation. Using an immunofluorescence assay, the results of the study directly showed that the average fluorescence intensity of RbAp48 in each cell reached an almost 1.7-fold increase at 72 and 96 h. This was consistent with the western blot results of RbAp48 during MEL differentiation. In addition, reduced expression of RbAp48 by RNA inference decreased SB-induced MEL differentiation by ~20%, indicating that a high level of RbAp48 was essential for MEL differentiation. Taken together, these results established a functional link between RbAp48 and erythroid differentiation.
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Wu-Lin
authors.initials: WL
authors.lastname: Qi
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Cao
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Jiang-Jiang
authors.initials: JJ
authors.lastname: Hu
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Jian-You
authors.initials: JY
authors.lastname: Xue
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Sang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Ya-Juan
authors.initials: YJ
authors.lastname: Zheng
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Fu-Kun
authors.initials: FK
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Shi-Fu
authors.initials: SF
authors.lastname: Zhang
keywords: RbAp48; erythroid differentiation; murine erythroleukemia cell; sodium butyrate