title: Growth and replication of infectious bursal disease virus in the DF-1 cell line and chicken embryo fibroblasts.
abstract: Infectious bursal disease virus (IBDV) causes a highly contagious disease in young chicks and leads to significant economic losses in the poultry industry. To determine a suitable cell line for IBDV infection, replication, and growth kinetics of the virus, DF-1 cells and chicken embryo fibroblasts (CEF) were used. The population doubling per day (Pd/D) was found to be higher in DF-1 as compared to CEF cells. A suitable time of infection (TOI) was established for increased production of virus and greater infectivity titers. The DF-1 and CEF cells were found to be susceptible to infection by producing marked cytopathic effects (CPEs), and the growth curves of IBDV in DF-1 and CEF cells were evaluated by infectivity assay using tissue culture infectious dose (TCID50). The cytopathic effects of the virus in DF-1 and CEF cells were found to be similar, but higher viral titers were detected in the DF-1 cells as compared to CEF. Thus the DF-1 cell line had a higher growth potential and infectivity, which will be of advantage in vaccine production.
authors.affiliation: Department of Environmental and Herbal Science, Tamil University, Thanjavur, Tamil Nadu 613005, India.
authors.forename: Kaliyaperumal
authors.initials: K
authors.lastname: Rekha
authors.affiliation: Department of Environmental and Herbal Science, Tamil University, Thanjavur, Tamil Nadu 613005, India.
authors.forename: Chandran
authors.initials: C
authors.lastname: Sivasubramanian
authors.affiliation: Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 143701, Republic of Korea.
authors.forename: Ill-Min
authors.initials: IM
authors.lastname: Chung
authors.affiliation: Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 143701, Republic of Korea.
authors.forename: Muthu
authors.initials: M
authors.lastname: Thiruvengadam
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000235:genetics / Q000662:veterinary / Q000821:virology*; D002460:Cell Line; D002642:Chick Embryo / Q000821:virology; D002645:Chickens / Q000821:virology; D004261:DNA Replication / Q000235:genetics; D005347:Fibroblasts / Q000821:virology; D007243:Infectious bursal disease virus / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity