title: Lignan formation in hairy root cultures of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter).
abstract: A hairy root line of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter) was obtained upon transformation with Agrobacterium rhizogenes strain ATCC15834. Elicitation of this line with silver nitrate, sucrose, methyl jasmonate and yeast extract at various concentrations in most cases resulted in a stimulation of lignan biosynthesis. Through elicitation with 6% sucrose the roots accumulated the pharmacologically active lignans leoligin and 5-methoxy-leoligin at levels of 0.0678% and 0.0372%, respectively, without significant growth inhibition. These lignan levels were comparable to those found in intact roots of cultivated Edelweiss. The biotechnological production of leoligin could be an attractive option for the continuous, field culture-independent production of the valuable secondary metabolites leoligin and 5-methoxy-leoligin.
authors.affiliation: Department of Pharmacognosy, University of Vienna, Althanstr. 14, A-1090 Vienna, Austria. Electronic address: christoph.wawrosch@univie.ac.at.
authors.forename: Christoph
authors.initials: C
authors.lastname: Wawrosch
authors.affiliation: Institute of Pharmacy/Pharmacognosy, University of Innsbruck, Josef-Moeller-Haus, Innrain 52c, A-6020 Innsbruck, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwaiger
authors.affiliation: Institute of Pharmacy/Pharmacognosy, University of Innsbruck, Josef-Moeller-Haus, Innrain 52c, A-6020 Innsbruck, Austria.
authors.forename: Hermann
authors.initials: H
authors.lastname: Stuppner
authors.affiliation: Department of Pharmacognosy, University of Vienna, Althanstr. 14, A-1090 Vienna, Austria.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Kopp
mesh_terms: D000085:Acetates; D060054:Agrobacterium / Q000502:physiology; D019659:Asteraceae / Q000378:metabolism* / Q000382:microbiology; D046508:Culture Techniques; D003517:Cyclopentanes; D017705:Lignans / Q000378:metabolism*; D054883:Oxylipins; D018517:Plant Roots / Q000378:metabolism* / Q000382:microbiology
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D017705:Lignans; D054883:Oxylipins; C542788:leoligin; C072239:methyl jasmonate
keywords: 5-Methoxy-leoligin; Agrobacterium rhizogenes; Edelweiss; Elicitor; Hairy roots; Leoligin; Leontopodium nivale ssp. alpinum; Lignan