title: Isolation of Leptospira interrogans Hardjoprajitno from vaginal fluid of a clinically healthy ewe suggests potential for venereal transmission.
abstract: A total of 15 adult ewes from one flock known to be seroreactive for leptospirosis was studied. Urine and vaginal fluid were collected from each animal to test for the presence of leptospires using bacterial culture and conventional PCR methods. One pure culture of Leptospira sp. was obtained from the vaginal fluid sample of a non-pregnant ewe. The isolate was characterized by DNA sequencing of the rrs and secY genes, variable-number of tandem-repeats (VNTR) analysis and serogrouping, and the isolate was typed as Leptospira interrogans serogroup Sejroe serovar Hardjo type Hardjoprajitno. This report indicates the presence of viable Leptospira in the vaginal fluid of a ewe, suggesting the potential for venereal transmission of leptospires in sheep.
authors.affiliation: Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niterói, RJ, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Director
authors.affiliation: Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niterói, RJ, Brazil.
authors.forename: B
authors.initials: B
authors.lastname: Penna
authors.affiliation: Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niterói, RJ, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Hamond
authors.affiliation: Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niterói, RJ, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Loureiro
authors.affiliation: Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niterói, RJ, Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Martins
authors.affiliation: Recombinant Technology Laboratory, Bio-Manguinhos, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Av. Brasil, 4365 - Manguinhos, Rio de Janeiro, RJ 21040-360, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Medeiros
authors.affiliation: Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niterói, RJ, Brazil.
authors.forename: W
authors.initials: W
authors.lastname: Lilenbaum
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D012634:Bodily Secretions / Q000382:microbiology*; D005260:Female; D007921:Leptospira interrogans / Q000302:isolation & purification*; D007922:Leptospirosis / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D017422:Sequence Analysis, DNA; D012749:Sexually Transmitted Diseases / Q000382:microbiology / Q000662:veterinary; D012756:Sheep / Q000382:microbiology*; D012757:Sheep Diseases / Q000382:microbiology*; D014621:Vagina / Q000382:microbiology*