title: Effects of pre-irradiation and SDF-1 suppression on the progression of murine astrocytoma cells grown in different stromal beds.
abstract: OBJECTIVE
  To examine whether brain tumors grown in pre-irradiated (PreIR) thigh have a similar tumor bed effect (TBE) as in PreIR brain tissue.
  
  
  METHODS
  Tumor growth delay and immunohistochemical (IHC) staining for CD31, an endothelial surface marker, and PIMO, a hypoxia marker, were used to study the TBE of a murine astrocytoma, ALTS1C1, or a stromal-derived factor-1 (SDF-1) gene-silenced astrocytoma, ALTS1C1-SDFkd, growing in different PreIR stroma beds.
  
  
  RESULTS
  ALTS1C1 tumors growing in both PreIR brain and PreIR thigh had reduced microvascular density (MVD) and more chronic hypoxia, but tumor growth delay was only seen in PreIR brain tissue. In contrast, ALTS1C1-SDFkd tumors showed tumor growth delay in PreIR thigh, with little effect in PreIR brain tissue.
  
  
  CONCLUSIONS
  This study cautions that both the tumor and the nature of the PreIR stromal bed are important when using pre-irradiation as a model of recurrent brain tumors after radiation therapy.
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing-Hua University , Hsinchu , Taiwan.
authors.forename: Ching-Fang
authors.initials: CF
authors.lastname: Yu
authors.forename: Chi-Min
authors.initials: CM
authors.lastname: Lin
authors.forename: Shu-Chi
authors.initials: SC
authors.lastname: Wang
authors.forename: Fang-Hsin
authors.initials: FH
authors.lastname: Chen
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Hong
authors.forename: Chien-Sheng
authors.initials: CS
authors.lastname: Tsai
authors.forename: Ying-Chieh
authors.initials: YC
authors.lastname: Yang
authors.forename: Chi-Shiun
authors.initials: CS
authors.lastname: Chiang
mesh_terms: D000818:Animals; D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology* / Q000532:radiotherapy; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000532:radiotherapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic; D054377:Chemokine CXCL12 / Q000172:deficiency* / Q000235:genetics*; D020868:Gene Silencing; D051379:Mice; D008810:Mice, Inbred C57BL; D017154:Stromal Cells / Q000473:pathology; D013848:Thigh / Q000473:pathology
chemical_list: D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse
keywords: Glioma; SDF-1; radiation therapy