title: Evidence for peroxidase activity in Caralluma umbellata.
abstract: Vast applications of peroxidases create an increasing demand to characterize peroxidases from new sources with more applicability potential. The aim of the present study was to check the presence of peroxidase activity from Caralluma umbellata. This is the first report on the C. umbellata peroxidase (CUP). The presence of peroxidase was revealed by the histochemical analysis of the stem sections, zymographic studies, and in vitro peroxidase activity assay using various reducing substrates viz., 2, 2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), guaiacol, o-dianisidine, and ferulic acid. The band pattern in zymogram confirms that CUP has a molecular weight less than that of horseradish peroxidase (44 kDa). Comparative evaluation of peroxidase activity of CUP with respect to horseradish peroxidase (HRP) indicates that CUP catalyzes ABTS and ferulic acid in a similar pattern as HRP but with guaiacol, the extent of catalysis shown by CUP over HRP is high. The standard inhibitors sodium azide and sodium meta bisulphite inhibited CUP activity in a dose dependent manner.
authors.affiliation: Department of Biotechnology, JSS Technical Institutions Campus, Mysore, Sri Jayachamarajendra College of Engineering, Karnataka, 570 006, India.
authors.forename: Raghu Ram
authors.initials: RR
authors.lastname: Achar
authors.forename: B K
authors.initials: BK
authors.lastname: Venkatesh
authors.forename: P
authors.initials: P
authors.lastname: Sharanappa
authors.forename: B S
authors.initials: BS
authors.lastname: Priya
authors.forename: S Nanjunda
authors.initials: SN
authors.lastname: Swamy
mesh_terms: D029062:Apocynaceae / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D055162:Biocatalysis; D003373:Coumaric Acids / Q000737:chemistry / Q000378:metabolism; D006139:Guaiacol / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D010544:Peroxidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D003373:Coumaric Acids; D010940:Plant Proteins; D006139:Guaiacol; C004999:ferulic acid; D010544:Peroxidases