title: Isolation and purification of a polysaccharide from the caterpillar medicinal mushroom Cordyceps militaris (Ascomycetes) fruit bodies and its immunomodulation of RAW 264.7 macrophages.
abstract: A novel polysaccharide (CP2-S) was purified from Cordyceps militaris fruit bodies by hot water extraction, ethanol precipitation, DEAE-Sepharose Fast Flow and Sephacryl S-400 high-resolution chromatography. The polysaccharide had a molecular weight of 5.938 × 10(6) g/mol and was mainly composed of glucose. CP2-S had carbohydrate content estimated to be 100% using the phenol-sulfuric acid method. Immunostimulating experiments in vitro indicated that CP2-S could stimulate nitric oxide production, phagocytosis, respiratory burst activity, and secretion of interleukin-1β and interleukin-2 of macrophages, suggesting that this water-soluble polysaccharide from the fruit body of C. militaris is a natural immunostimulating polysaccharide with potential for further application.
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungi Processing.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Insitute of Edible Fungi,Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization, Shanghai Key Laboratory of Agricultural Genetics and Breeding, China.
authors.forename: Qingjiu
authors.initials: Q
authors.lastname: Tang
authors.affiliation: National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Insitute of Edible Fungi,Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization, Shanghai Key Laboratory of Agricultural Genetics and Breeding, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Eco-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Gao
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Zhaolong
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D032661:Cordyceps / Q000737:chemistry* / Q000254:growth & development; D048690:Fruiting Bodies, Fungal / Q000737:chemistry / Q000254:growth & development; D007155:Immunologic Factors / Q000302:isolation & purification* / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D007155:Immunologic Factors; D053583:Interleukin-1beta; D007376:Interleukin-2; D011134:Polysaccharides