title: In vitro activation of NAD-dependent alcohol dehydrogenases by Nudix hydrolases is more widespread than assumed.
abstract: In the Gram-positive methylotroph Bacillus methanolicus, methanol oxidation is catalyzed by an NAD-dependent methanol dehydrogenase (Mdh) that belongs to the type III alcohol dehydrogenase (Adh) family. It was previously shown that the in vitro activity of B. methanolicus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase. Here we show that this feature is not unique, but more widespread among type III Adhs in combination with Act or other Act-like Nudix hydrolases. In addition, we studied the effect of site directed mutations in the predicted active site of Mdh and two other type III Adhs with regard to activity and activation by Act.
authors.affiliation: Institute of Microbiology, ETH Zurich, Switzerland.
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Ochsner
authors.affiliation: Institute of Microbiology, ETH Zurich, Switzerland.
authors.forename: Jonas E N
authors.initials: JE
authors.lastname: Müller
authors.affiliation: Institute of Microbiology, ETH Zurich, Switzerland.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Mora
authors.affiliation: Institute of Microbiology, ETH Zurich, Switzerland. Electronic address: vorholt@micro.biol.ethz.ch.
authors.forename: Julia A
authors.initials: JA
authors.lastname: Vorholt
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology*; D020134:Catalytic Domain; D004789:Enzyme Activation; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009243:NAD / Q000378:metabolism*; D011755:Pyrophosphatases / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D000096964:Nudix Hydrolases
chemical_list: D000429:Alcohol Oxidoreductases; D009243:NAD; D011755:Pyrophosphatases; D000096964:Nudix Hydrolases; C019641:alcohol dehydrogenase (acceptor)
keywords: Alcohol dehydrogenase; Bacillus methanolicus; Methylotrophy