title: Isolation of West Nile virus from urine samples of patients with acute infection.
abstract: This study demonstrated that West Nile virus (WNV) excreted in the urine of patients with acute infection can be isolated in cell cultures. In addition, the protocols for WNV isolation from urine samples were standardized, and factors that may affect the efficiency of WNV isolation were identified.
authors.affiliation: Department of Molecular Medicine, University of Padua, Padua, Italy Microbiology and Virology Unit, Padua University Hospital, Padua, Italy luisa.barzon@unipd.it.
authors.forename: Luisa
authors.identifier: http://orcid.org/0000-0003-0720-8456
authors.initials: L
authors.lastname: Barzon
authors.affiliation: Microbiology and Virology Unit, Padua University Hospital, Padua, Italy.
authors.forename: Monia
authors.initials: M
authors.lastname: Pacenti
authors.affiliation: Department of Molecular Medicine, University of Padua, Padua, Italy Microbiology and Virology Unit, Padua University Hospital, Padua, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Franchin
authors.affiliation: Department of Molecular Medicine, University of Padua, Padua, Italy Microbiology and Virology Unit, Padua University Hospital, Padua, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Squarzon
authors.affiliation: Microbiology and Virology Unit, Padua University Hospital, Padua, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sinigaglia
authors.affiliation: Department of Immunology, Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Ulbert
authors.affiliation: Microbiology and Virology Unit, Padua University Hospital, Padua, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Cusinato
authors.affiliation: Department of Molecular Medicine, University of Padua, Padua, Italy Microbiology and Virology Unit, Padua University Hospital, Padua, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Palù
mesh_terms: D003955:Diagnostic Tests, Routine / Q000379:methods / Q000592:standards; D006801:Humans; D014556:Urine / Q000821:virology*; D014776:Virus Cultivation / Q000379:methods / Q000592:standards; D014901:West Nile Fever / Q000821:virology*; D014902:West Nile virus / Q000302:isolation & purification*