title: JAK2, STAT3 and SOCS3 gene expression in women with and without breast cancer.
abstract: BACKGROUND
  Breast cancer is a disease that arises from the accumulation of alterations in the genome of cells that make up the mammary gland. The Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) signaling pathway has been reported to participate in the development of breast cancer and is activated by adipocytokines such as leptin, which are elevated in obesity. In contrast, alterations in suppressor of cytokine signaling 3 (SOCS3) gene expression have been found in patients with decreased breast cancer metastasis.
  
  
  OBJECTIVE
  The current study sought to identify whether JAK2, STAT3 and SOCS3 gene expression is associated with body mass index (BMI) and breast cancer.
  
  
  METHODS
  This was a cross-sectional prospective study. JAK2, STAT3 and SOCS3 gene expression levels were determined using RT-qPCR from the biopsies of 26 patients with breast cancer and 43 patients with benign breast lesions. We compared the expression of these genes, relative to the housekeeping genes, ACTB and GAPDH, against BMI, clinical stage and immunohistochemistry.
  
  
  RESULTS
  STAT3 gene expression was increased in breast cancer patients (p≤0.001; AUC=0.65; AUC 95% CI: 0.5-0.8), and SOCS3 expression was decreased in obese patients with benign breast lesions (p≤0.001; AUC=0.51; AUC 95% CI: 0.36-0.65). With regard to the clinical stage, there were significant differences in STAT3 gene expression between stage II and III (p≤0.011) and stage II and IV (p≤0.033) breast cancers. Among all women, there was a positive correlation between JAK2 and STAT3 expression (R=0.493, p=0.000). In addition, breast cancers that were negative for HER2 were associated with JAK2 and SOCS3 (R=0.645, p=0.003).
  
  
  CONCLUSIONS
  High levels of STAT3 expression were associated with early stages of breast cancer development and patients in the control group with obesity showed higher expression of SOCS3 regarding overweight.
authors.affiliation: Molecular Biology Laboratory, Medical Research Center (CICMED), Autonomous University of the State of Mexico (UAEMex), Toluca, México C.P. 50130, Mexico; Toxicology Laboratory, Pharmacy Department, Faculty of Chemistry, UAEMex, Toluca, México C.P. 50120, Mexico. Electronic address: jonnathangsb@yahoo.com.mx.
authors.forename: Jonnathan Guadalupe
authors.initials: JG
authors.lastname: Santillán-Benítez
authors.affiliation: Molecular Biology Laboratory, Medical Research Center (CICMED), Autonomous University of the State of Mexico (UAEMex), Toluca, México C.P. 50130, Mexico. Electronic address: mezh_74@yahoo.com.
authors.forename: Hugo
authors.initials: H
authors.lastname: Mendieta-Zerón
authors.affiliation: Toxicology Laboratory, Pharmacy Department, Faculty of Chemistry, UAEMex, Toluca, México C.P. 50120, Mexico. Electronic address: lmgomezo@uaemex.mx.
authors.forename: Leobardo Manuel
authors.initials: LM
authors.lastname: Gómez-Oliván
authors.affiliation: Toxicology Laboratory, Pharmacy Department, Faculty of Chemistry, UAEMex, Toluca, México C.P. 50120, Mexico. Electronic address: aoq_27@yahoo.com.mx.
authors.forename: Angel
authors.initials: A
authors.lastname: Ordóñez Quiroz
authors.affiliation: Maternal-Perinatal Hospital "Mónica Pretelini" (HMPMP), Toluca, México, C.P. 50130, Mexico. Electronic address: juantorresmd@yahoo.com.mx.
authors.forename: Juan Jueventino
authors.initials: JJ
authors.lastname: Torres-Juárez
authors.affiliation: Maternal-Perinatal Hospital "Mónica Pretelini" (HMPMP), Toluca, México, C.P. 50130, Mexico. Electronic address: zagi_jm@hotmail.com.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: González-Bañales
mesh_terms: D000328:Adult; D015992:Body Mass Index; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011446:Prospective Studies; D050796:STAT3 Transcription Factor / Q000235:genetics*; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics*
chemical_list: C106644:SOCS3 protein, human; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; C507924:JAK2 protein, human; D053614:Janus Kinase 2
keywords: Body mass index (BMI); Breast cancer; JAK2; SOCS3; STAT3