title: Dendritic cells transduced with CPEB4 induced antitumor immune response.
abstract: Much evidence leads to the exploration of immunologic approaches for eliminating tumor cells. Cytoplasmic polyadenylation element binding protein 4 (CPEB4) is considered to be a novel therapeutical target for glioblastoma. In this study, we transduced DCs with CPEB4 to explore the immune response in vivo. We found that DCs transduced with recombinant adenovirus encoding CPEB4 could induce specific cytotoxic T lymphocytes (CTLs) to lyse glioma cells and augment the number of IFN-γ secreting T-cells in mice. In addition, the modified DCs could effectively protect mice from lethal challenges against glioma cells, reduce tumor growth and increase the mice life span. These results suggest that the DC transduced with CPEB4 may induce anti-tumor immunity against glioma cells and might be used as an efficient tumor vaccine in clinical applications.
authors.affiliation: Department of Orthopaedics, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.affiliation: Department of Urology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Nan
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Hanchao
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Chuangan
authors.initials: C
authors.lastname: Lin
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China. Electronic address: ytt83812280@sina.com.
authors.forename: Bangqing
authors.initials: B
authors.lastname: Yuan
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005910:Glioma / Q000628:therapy; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism
chemical_list: D019496:Cancer Vaccines; C477604:Cpeb4 protein, mouse; D016601:RNA-Binding Proteins; D007371:Interferon-gamma
keywords: Antitumor; CPEB4; Dendritic cells; Immune response