title: The relationship between clinicopathological features and expression of epithelial and mesenchymal markers in spontaneous canine mammary gland tumors.
abstract: It is known that epithelial mesenchymal transition (EMT) contributes to the acquisition of malignant property in human cancers. However, the role of EMT in canine tumors remains to be elucidated. To evaluate the correlation between expression levels of protein markers involved in EMT and clinicopathological characteristics in canine mammary gland tumors, immunohistochemistry using antibodies against ZO-1, E-cadherin, vimentin, N-cadherin and fibronectin was performed on 119 clinical tissue samples. Consequently, loss of ZO-1 and E-cadherin, and gain of vimentin and N-cadherin were more frequently observed in malignant tumors than in benign tumors. However, there was no correlation among expression of these molecules. Univariate and multivariate analysis identified that loss of E-cadherin independently had a low one-year survival rate (adjusted odds ratio: 2.3, P=0.02). These results suggested that EMT might relate to acquisition of malignancy, and additionally, E-cadherin was strongly correlated with malignant behavior in canine mammary gland tumors.
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Yoshida
authors.forename: Saori
authors.initials: S
authors.lastname: Yoshida
authors.forename: Nan
authors.initials: N
authors.lastname: Choisunirachon
authors.forename: Tomochika
authors.initials: T
authors.lastname: Saito
authors.forename: Kaori
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Kohei
authors.initials: K
authors.lastname: Saeki
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nishimura
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakagawa
mesh_terms: D000230:Adenocarcinoma / Q000145:classification / Q000378:metabolism / Q000662:veterinary*; D000236:Adenoma / Q000145:classification / Q000378:metabolism / Q000662:veterinary*; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D004283:Dog Diseases / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor