title: Effect of the organic loading rate on the production of polyhydroxyalkanoates in a multi-stage process aimed at the valorization of olive oil mill wastewater.
abstract: Mixed microbial culture polyhydroxyalkanoates (PHA) production has been investigated by using olive oil mill wastewater (OMW) as no-cost feedstock in a multi-stage process, also involving phenols removal and recovery. The selection of PHA-storing microorganisms occurred in a sequencing batch reactor (SBR), fed with dephenolized and fermented OMW and operated at different organic loading rates (OLR), ranging from 2.40 to 8.40gCOD/Ld. The optimal operating condition was observed at an OLR of 4.70gCOD/Ld, which showed the highest values of storage rate and yield (339±48mgCOD/gCODh and 0.56±0.05 COD/COD, respectively). The OLR applied to the SBR largely affected the performance of the PHA-accumulating reactor, which was fed through multiple pulsed additions of pretreated OMW. From an overall mass balance, involving all the stages of the process, an abatement of about 85% of the OMW initial COD (chemical oxygen demand) was estimated whereas the conversion of the influent COD into PHA was about 10% (or 22% by taking into account only the COD contained in the pretreated OMW, which is directly fed to the PHA production stages). Overall, polymer volumetric productivity (calculated from the combination of both the SBR and the accumulation reactor) accounted for 1.50gPHA/Ld.
authors.affiliation: Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Campanari
authors.affiliation: Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Francisca A
authors.initials: FA
authors.lastname: e Silva
authors.affiliation: Department of Civil, Chemical, Environmental and Materials Engineering, Alma Mater Studiorum, University of Bologna, Via Terracini 28, 40131 Bologna, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Bertin
authors.affiliation: Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. Electronic address: marianna.villano@uniroma1.it.
authors.forename: Marianna
authors.initials: M
authors.lastname: Villano
authors.affiliation: Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Majone
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors; D005285:Fermentation; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D013997:Time Factors; D062065:Wastewater / Q000737:chemistry*
chemical_list: D000069463:Olive Oil; D010938:Plant Oils; D054813:Polyhydroxyalkanoates; D062065:Wastewater
keywords: Mixed microbial culture; Olive oil mill wastewater; Polyhydroxyalkanoate production