title: Profiling lysine ubiquitination by selective enrichment of ubiquitin remnant-containing peptides.
abstract: Protein ubiquitination plays critical roles in many biological processes. However, functional studies of protein ubiquitination in eukaryotic cells are limited by the ability to identify protein ubiquitination sites. Unbiased high-throughput screening methods are necessary to discover novel ubiquitination sites that play important roles in cellular regulation. Here, we describe an immunopurification approach that enriches ubiquitin remnant-containing peptides to facilitate downstream mass spectrometry (MS) identification of lysine ubiquitination sites. This approach can be utilized to identify ubiquitination sites from proteins in a complex mixture.
authors.affiliation: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Xu
authors.forename: Alessia
authors.initials: A
authors.lastname: Deglincerti
authors.forename: Jeremy S
authors.initials: JS
authors.lastname: Paige
authors.forename: Samie R
authors.initials: SR
authors.lastname: Jaffrey
mesh_terms: D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D008239:Lysine / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D025801:Ubiquitin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D057149:Ubiquitinated Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D010455:Peptides; D025801:Ubiquitin; D057149:Ubiquitinated Proteins; D008239:Lysine