title: [Expression of peroxisome proliferators-activated receptor in glioma and its effect on the growth of human glioma cells].
abstract: OBJECTIVE
  To study the expression of peroxisome proliferators-activated receptor (PPAR) in human glioma tissue and its influence on tumor growth.
  
  
  METHODS
  Expression of PPAR mRNA in glioma tissue was determined by real-time reverse transcription polymerase chain reaction (RT-PCR). Subsequently, MTT (3-(4, 5)-dimethylthiahiazo(-z-y1)-3, 5-di-phenytetrazoliumromide) assay, flow cytometry, reactive oxygen species assay kit and Western blotting were used to assay U87 cells with agonist activity of PPAR.
  
  
  RESULTS
  The data demonstrated that the expression of PPAR in glioma was low and negatively correlated with its pathological grade. Activation of PPAR suppresses tumor cell proliferation, delays the cell cycle at G1 phrase, and induces apoptosis and accumulation of reactive oxygen species (ROS) in U87 cells.
  
  
  CONCLUSIONS
  The expression of PPAR mRNA in human glioma was low. PPAR protein plays a critical role in the progression of glioma via the PPAR signal pathway.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China. Email: yypl9@njmu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Wenkang
authors.initials: W
authors.lastname: Luan
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
authors.forename: Jiajia
authors.initials: J
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Dong
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Yongping
authors.initials: Y
authors.lastname: You
mesh_terms: D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015870:Gene Expression; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D047493:PPAR alpha / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D047493:PPAR alpha