title: Article expression, purification, and characterization of Cu/ZnSOD from Panax ginseng.
abstract: Superoxide dismutase (SOD) has a strong antioxidant effect, but the traditional SOD extraction method is not the most efficient method of SOD amplification. In this study, we report the cloning of the Cu/ZnSOD gene from Panax ginseng into a temperature-regulated expression plasmid, pBV220. Cu/ZnSOD inclusion bodies were expressed in E. coli at a high level. Then, the inclusion bodies were purified by ion-exchange chromatography and molecular sieve chromatography. Finally, we obtained stable SOD in the bacterial broth, with a protein content of 965 mg/L and enzyme specific activity of 9389.96 U/mg. These results provide a foundation for future studies on the antioxidant mechanisms of ginseng and the development and application of ginseng Cu/ZnSOD.
authors.affiliation: Department of Gastrointestinal Surgery, China-Japan Union Hospital, Jilin University, Changchun 130000, China. dyangel89@163.com.
authors.forename: Dayong
authors.initials: D
authors.lastname: Ding
authors.affiliation: Center of Chinese Medicine and Bio-Engineering Research, Changchun University of Chinese Medicine, Changchun 130117, China. lsc9668@163.com.
authors.forename: Shichao
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Rheumatoid Immune, First Hospital of Jilin University, Changchun 130000, China. caikexin5258@126.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Geriatrics, China-Japan Union Hospital, Jilin University, Changchun 130000, China. 18946726604@163.com.
authors.forename: Lihong
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Gastrointestinal Surgery, China-Japan Union Hospital, Jilin University, Changchun 130000, China. lwsm126030@126.com.
authors.forename: Jisheng
authors.initials: J
authors.lastname: Zhao
mesh_terms: D002852:Chromatography, Ion Exchange; D004926:Escherichia coli / Q000378:metabolism; D005894:Panax / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: D013482:Superoxide Dismutase