title: Chiral separation of 12 cathinone analogs by cyclodextrin-assisted capillary electrophoresis with UV and mass spectrometry detection.
abstract: In this study, a rapid chiral separation of 12 cathinones analogs has been developed and validated using cyclodextrin-assisted CE with UV and TOF-MS detection. Optimum separation was obtained on a 57.5 cm × 50 μm capillary using a buffer system consisting of 10 mM β-cyclodextrin (β-CD) in a 100 mM phosphate buffer for CE-UV, and 0.6% v/v highly sulfated-γ-cyclodextrin (HS-γ-CD) in a 50 mM phosphate buffer for CE-MS. In the CE-MS experiment, a partial filling technique was employed to ensure that a minimum amount of cyclodextrin entered the mass spectrometer. All analytes were separated within 18 min in the CE-UV separation and identified by TOF-MS. Ten compounds were enantiomerically separated using β-CD in the UV mode and an additional two more were enantiomerically separated using HS-γ-CD in the MS mode. Detection limits down to 1.0 ng/mL were obtained. The method was then applied to examine seized drugs.
authors.affiliation: Department of Chemistry, Florida International University, Miami, FL, USA; Department of Public Health and Community Medicine, Unit of Forensic Medicine, University of Verona, Verona, Italy; Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Merola
authors.forename: Hanzhuo
authors.initials: H
authors.lastname: Fu
authors.forename: Franco
authors.initials: F
authors.lastname: Tagliaro
authors.forename: Teodora
authors.initials: T
authors.lastname: Macchia
authors.forename: Bruce R
authors.initials: BR
authors.lastname: McCord
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D015198:Designer Drugs / Q000032:analysis / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D015198:Designer Drugs; D047392:beta-Cyclodextrins; C023665:cathinone
keywords: CE; Cathinone analogs; Chiral separation; Cyclodextrin; MS