title: [Effects of transforming growth factor-β1 gene silencing on the expression of vascular endothelial growth factor in human bladder cancer cell lines].
abstract: OBJECTIVE
  To employ RNA interference technology to silence transforming growth factor-β1 (TGF-β1) gene to examine the gene silencing effects of RNAi on the expression of vascular endothelial growth factor (VEGF) in human bladder cancer cell lines (EJ).
  
  
  METHODS
  The TGF-β1 gene-specific siRNA expression vector was constructed. And the most efficiently suppressed target sequences were screened through reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The samples were divided into 3 groups of EJ, control (TGF-β1) and recombinant plasmid (TGF-β1 siRNA expression vector). And the expression level of VEGF protein was detected by Western blot.
  
  
  RESULTS
  TGF-β1 gene-specific siRNA expression vector was constructed successfully. TGF-β1 relative mRNA expression was 0.92 ± 0.19 and the protein expression level (50 ± 6) pg/ml. The protein expression level of EJ group after transfection was (0.86 ± 0.18) pg/ml, control group (1.15 ± 0.29) pg/ml and recombinant plasmid group (0.45 ± 0.16) pg/ml(both P < 0.05).
  
  
  CONCLUSIONS
  An inhibition of TGF-β1 gene down-regulates the expression of VEGF. And TGF-β1 may regulate angiogenesis of bladder tumor through an induction of VEGF gene expression.
authors.affiliation: Department of Urology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Urology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, Chengdu 610072, China. Email: mingxingqiu@gmail.com.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Qiu
authors.affiliation: Department of Urology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Urology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Huang
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D053773:Transforming Growth Factor beta1 / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A