title: Effects of sample treatments on genome recovery via single-cell genomics.
abstract: Single-cell genomics is a powerful tool for accessing genetic information from uncultivated microorganisms. Methods of handling samples before single-cell genomic amplification may affect the quality of the genomes obtained. Using three bacterial strains we show that, compared to cryopreservation, lower-quality single-cell genomes are recovered when the sample is preserved in ethanol or if the sample undergoes fluorescence in situ hybridization, while sample preservation in paraformaldehyde renders it completely unsuitable for sequencing.
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Clingenpeel
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA, USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Schwientek
authors.affiliation: Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Philip
authors.initials: P
authors.lastname: Hugenholtz
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA, USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
mesh_terms: D015925:Cryopreservation; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence; D059010:Single-Cell Analysis