title: Role of the clinical pathology laboratory in the evaluation of endometrial carcinomas for Lynch syndrome.
abstract: Molecular diagnostic testing of endometrial carcinomas in the pathology laboratory has recently emerged as a key component of the clinical evaluation of Lynch syndrome in many centers. Testing modalities involve immunohistochemical and PCR-based analyses. This article outlines the routine application of these analyses, provides a practical guide for troubleshooting some of the common technical issues related to their performance, and reviews common pitfalls in their interpretation. Discrepancies between tissue testing and genetic testing results are discussed in the context of the current understanding of endometrial cancer biology. The merits of universal versus targeted tissue testing based on clinical patient history and histological tumor appearance are also addressed.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Ottawa, The Ottawa Hospital, Eastern Ontario Regional Laboratory, 501 Smyth Road, Ottawa, Ontario, Canada K1H 8L6. Electronic address: bdjordjevic@toh.on.ca.
authors.forename: Bojana
authors.initials: B
authors.lastname: Djordjevic
authors.affiliation: Department of Pathology, University of Texas M.D. Anderson Cancer Center, Box 85, 1515 Holcombe Blvd, Houston, Texas 77030.
authors.forename: Russell R
authors.initials: RR
authors.lastname: Broaddus
mesh_terms: D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000175:diagnosis* / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D005260:Female; D005821:Genetic Techniques; D006801:Humans; D007150:Immunohistochemistry; D010338:Pathology, Clinical / Q000379:methods*
keywords: Lynch Syndrome; endometrial cancer; molecular diagnostic testing