title: Raman and coherent anti-Stokes Raman scattering microscopy studies of changes in lipid content and composition in hormone-treated breast and prostate cancer cells.
abstract: Increasing interest in the role of lipids in cancer cell proliferation and resistance to drug therapies has motivated the need to develop better tools for cellular lipid analysis. Quantification of lipids in cells is typically done by destructive chromatography protocols that do not provide spatial information on lipid distribution and prevent dynamic live cell studies. Methods that allow the analysis of lipid content in live cells are therefore of great importance. Using micro-Raman spectroscopy and coherent anti-Stokes Raman scattering (CARS) microscopy, we generated a lipid profile for breast (T47D, MDA-MB-231) and prostate (LNCaP, PC3) cancer cells upon exposure to medroxyprogesterone acetate (MPA) and synthetic androgen R1881. Combining Raman spectra with CARS imaging, we can study the process of hormone-mediated lipogenesis. Our results show that hormone-treated cancer cells T47D and LNCaP have an increased number and size of intracellular lipid droplets and higher degree of saturation than untreated cells. MDA-MB-231 and PC3 cancer cells showed no significant changes upon treatment. Principal component analysis with linear discriminant analysis of the Raman spectra was able to differentiate between cancer cells that were treated with MPA, R1881, and untreated.
authors.affiliation: University of Colorado Denver, Department of Bioengineering, Anschutz Medical Campus, Mail Stop 8607, 12700 East 19th Avenue, Aurora, Colorado 80045.
authors.forename: Mariana C
authors.initials: MC
authors.lastname: Potcoava
authors.affiliation: University of Colorado Denver, Department of Bioengineering, Anschutz Medical Campus, Mail Stop 8607, 12700 East 19th Avenue, Aurora, Colorado 80045.
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Futia
authors.affiliation: University of Colorado Denver, Department of Bioengineering, Anschutz Medical Campus, Mail Stop 8607, 12700 East 19th Avenue, Aurora, Colorado 80045.
authors.forename: Jessica
authors.initials: J
authors.lastname: Aughenbaugh
authors.affiliation: University of Colorado Denver, Department of Pharmacology, Anschutz Medical Campus, Mail Stop 8303, 12801 East 17th Avenue, Aurora, Colorado 80045.
authors.forename: Isabel R
authors.initials: IR
authors.lastname: Schlaepfer
authors.affiliation: University of Colorado Denver, Department of Bioengineering, Anschutz Medical Campus, Mail Stop 8607, 12700 East 19th Avenue, Aurora, Colorado 80045.
authors.forename: Emily A
authors.initials: EA
authors.lastname: Gibson
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D066292:Lipid Droplets / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D008297:Male; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D015741:Metribolone / Q000494:pharmacology; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D008055:Lipids; D015741:Metribolone; D017258:Medroxyprogesterone Acetate