title: Unique lamina propria stromal cells imprint the functional phenotype of mucosal dendritic cells.
abstract: Mucosal dendritic cells (DCs) in the intestine acquire the unique capacity to produce retinoic acid (RA), a vitamin A metabolite that induces gut tropism and regulates the functional differentiation of the T cells they prime. Here, we identified a stromal cell (SC) population in the intestinal lamina propria (LP), which is capable of inducing RA production in DCs in a RA- and granulocyte-macrophage colony-stimulating factor (GM-CSF)-dependent fashion. Unlike DCs, LP SCs constitutively expressed the enzymatic machinery to produce RA even in the absence of dietary vitamin A, but were not able to do so in germ-free mice implying regulation by microbiota. Interestingly, DCs promoted GM-CSF production by the SCs indicating a two-way cross-talk between both cell types. Furthermore, RA-producing LP SCs and intestinal DCs localized closely in vivo suggesting that the interactions between both cell types might have an important role in the functional education of migratory DCs and therefore in the regulation of immune responses toward oral and commensal antigens.
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: I
authors.initials: I
authors.lastname: Vicente-Suarez
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: A
authors.initials: A
authors.lastname: Larange
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: C
authors.initials: C
authors.lastname: Reardon
authors.affiliation: Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: M
authors.initials: M
authors.lastname: Matho
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: S
authors.initials: S
authors.lastname: Feau
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: G
authors.initials: G
authors.lastname: Chodaczek
authors.affiliation: 1] Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA [2] Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, Korea.
authors.forename: Y
authors.initials: Y
authors.lastname: Park
authors.affiliation: 1] Division of Mucosal Barriology, International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan [2] Laboratory for immune regulation, Graduate School of Medicine, Chiba University, Chiba, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Obata
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: R
authors.initials: R
authors.lastname: Gold
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang-Zhu
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: C
authors.initials: C
authors.lastname: Lena
authors.affiliation: Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Zajonc
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: S P
authors.initials: SP
authors.lastname: Schoenberger
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: M
authors.initials: M
authors.lastname: Kronenberg
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
authors.forename: H
authors.initials: H
authors.lastname: Cheroutre
mesh_terms: D000818:Animals; D002450:Cell Communication; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D004032:Diet; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D018928:Immunity, Mucosal; D056747:Immunomodulation; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009092:Mucous Membrane / Q000276:immunology*; D017154:Stromal Cells / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014212:Tretinoin / Q000276:immunology / Q000378:metabolism*
chemical_list: D014212:Tretinoin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor