title: Docosahexaenoic acid content is significantly higher in ghrita prepared by traditional Ayurvedic method.
abstract: BACKGROUND
  Ghee (clarified butter) also known as ghrita, has been utilized for thousands of years in Ayurveda. Ghee is mostly prepared by traditional method in Indian households or by direct cream method at industry level. Ayurvedic classics mention that ghrita made from cow milk is superior. However, there is no scientific comparison available on preparation methods and essential fatty acids content of ghrita.
  
  
  OBJECTIVE
  To investigate fatty acid composition of ghrita prepared by traditional/Ayurvedic method and commercial method (direct cream method).
  
  
  METHODS
  Fatty Acid Methyl Esters (FAME) extracted from ghrita samples were analysed on Gas Chromatography (GC) Shimadzu B using capillary column BPX70 (0.32 mm*60 m, ID of 0.25 mm). The fatty acids in the samples were identified by comparing peaks with the external standard 68A (Nu-Chek-Prep, Inc.USA). Significant differences between the experimental groups were assessed by analysis of variance.
  
  
  RESULTS
  Distribution of fatty acids was compared in ghrita samples prepared by traditional method and direct cream method which is commercially used. Saturated fatty acids were predominant in both the groups. Mono unsaturated fatty acids and poly unsaturated fatty acids were in the range of 17-18% and 3-6% respectively. DHA content was significantly higher in ghee prepared by traditional method using curd starter fermentation.
  
  
  CONCLUSIONS
  The findings suggested that ghrita prepared by traditional ayurvedic methods contains higher amount of DHA; Omega-3 long-chain polyunsaturated fatty acids, which is a major component of retinal and brain tissues and remains important in prevention of various diseases.
authors.affiliation: Department of Biotechnology, Sinhgad College of Engineering, Pune, Maharashtra, India.
authors.forename: Kalpana S
authors.initials: KS
authors.lastname: Joshi
keywords: Ayurveda; Docosahexaenoic acid; Omega-3 fatty acids; ghrita; traditional method