title: DNA microarray analysis of labial salivary glands in IgG4-related disease: comparison with Sjögren's syndrome.
abstract: OBJECTIVE
  To compare gene expression in labial salivary glands (LSGs) from patients with IgG4-related disease with that in LSGs from patients with Sjögren's syndrome (SS).
  
  
  METHODS
  Gene expression was analyzed by DNA microarray in LSG samples from 5 patients with IgG4-related disease, 5 SS patients, and 3 healthy controls. Genes differentially expressed in IgG4-related disease and SS were identified, and gene annotation enrichment analysis of these differentially expressed genes was performed using Gene Ontology (GO) annotation. Validation of the results was performed by quantitative polymerase chain reaction (PCR) using LSG samples from 9 patients with IgG4-related disease, 10 SS patients, and 4 controls.
  
  
  RESULTS
  Gene expression patterns in patients with IgG4-related disease, SS patients, and healthy controls were quite different from each other in hierarchical clustering as well as in principal components analysis. In IgG4-related disease compared with SS, a total of 1,771 probe sets (corresponding to 1,321 genes) were identified as up-regulated, and 1,785 probe sets (corresponding to 1,320 genes) were identified as down-regulated (false discovery rate of <5%). GO term analysis indicated that the up-regulated set of differentially expressed genes in IgG4-related disease encoded proteins that function in cell proliferation, extracellular matrix organization, and organ development. PCR validated significantly higher expression of lactotransferrin in patients with IgG4-related disease than in SS patients (P < 0.05) and significantly higher expression of CCL18 in patients with IgG4-related disease than in SS patients and controls (P < 0.05).
  
  
  CONCLUSIONS
  The results clearly showed that the gene expression pattern in LSGs from patients with IgG4-related disease is different from that in LSGs from SS patients.
authors.affiliation: University of Tsukuba, Ibaraki, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Tsuboi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakai
authors.forename: Mana
authors.initials: M
authors.lastname: Iizuka
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Asashima
authors.forename: Chihiro
authors.initials: C
authors.lastname: Hagiya
authors.forename: Sayaka
authors.initials: S
authors.lastname: Tsuzuki
authors.forename: Tomoya
authors.initials: T
authors.lastname: Hirota
authors.forename: Haruka
authors.initials: H
authors.lastname: Miki
authors.forename: Shinya
authors.initials: S
authors.lastname: Hagiwara
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kondo
authors.forename: Akihiko
authors.initials: A
authors.lastname: Tanaka
authors.forename: Masafumi
authors.initials: M
authors.lastname: Moriyama
authors.forename: Isao
authors.initials: I
authors.lastname: Matsumoto
authors.forename: Seiji
authors.initials: S
authors.lastname: Nakamura
authors.forename: Toshio
authors.initials: T
authors.lastname: Yoshihara
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sumida
mesh_terms: D000328:Adult; D000368:Aged; D001327:Autoimmune Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007074:Immunoglobulin G; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012469:Salivary Glands / Q000378:metabolism* / Q000473:pathology; D012859:Sjogren's Syndrome / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D055815:Young Adult
chemical_list: D007074:Immunoglobulin G