title: Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells.
abstract: The aim of the present study was to investigate the effects of aloe-emodin (AE) on the radiosensitivity and differentiation of HeLa human cervical cancer cells. Cell proliferation was assessed in the HeLa cervical cancer cell line by a methylthiazolyldiphenyl-tetrazolium bromide assay. Radiosensitivity was determined by a colony‑forming assay. Flow cytometry was used for analysis of cell cycle distribution and apoptosis. The expression of γ-H2AX and cyclin B was assessed by western blotting. Alkaline phosphatase (ALP) activity was measured by an ALP activity kit. It was demonstrated that AE inhibited the proliferation of HeLa cells in a concentration- and time-dependent manner, induced G2/M and S phase cell cycle arrest and enhanced the radiosensitivity of HeLa cells. The combination of AE and radiation induced apoptosis, upregulated cyclin B and γ-H2AX expression and further improved ALP activity compared with treatment with AE or radiation alone. AE enhanced the radiosensitivity of HeLa human cervical cancer cells in vitro, inhibited the proliferation of HeLa cells, induced G2/M phase cell cycle arrest and, in combination with radiation, induced the apoptosis and improved the differentiation of HeLa cells.
authors.affiliation: Department of Radiotherapy, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Gynecology, The Tumor Hospital of Jilin, Changchun, Jilin 130021, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Radiotherapy, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Pengyu
authors.initials: P
authors.lastname: Chang
authors.affiliation: Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Radiotherapy, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Qu
mesh_terms: D000880:Anthraquinones / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002454:Cell Differentiation / Q000187:drug effects* / Q000528:radiation effects; D019926:Cyclin B / Q000378:metabolism; D005260:Female; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects / Q000528:radiation effects; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D000880:Anthraquinones; D019926:Cyclin B; D006657:Histones; C518327:aloe emodin