title: [Blueberry anthocyanins induce G2/M cell cycle arrest and apoptosis of oral cancer KB cells through down-regulation methylation of p53].
abstract: Blueberries are an excellent source of dietary polyphenols such as anthocyanins and phenolic acids. In this study, we investigated the ability of anthocyanins from the wild blueberries of Inner Mongolia to suppress the growth of the oral cancer cell line KB. The blueberry anthocyanins were extracted with methanol-containing 0.1% (v/v) hydrochloric acid. Fourteen unique anthocyanins were identified using high-performance liquid chromatography-mass spectrometry (HPLC-MS). The anticancer bioactivity of the extracts on KB cells was analyzed using methylthiazolyl-tetrazolium (MTT), flow cytometry (FCM) and immunocytochemistry. It was shown that the blueberry anthocyanins suppressed the proliferation of KB cells in a dose-dependent manner, as well as induced G2/M cell cycle arrest and apoptosis of oral cancer KB cells. Immunocytochemistry analysis showed that the expression of caspase-9 and cytochrome c were obviously increased after the anthocyanins treatment. Western blot analysis also indicated that the expression of p53 was increased. Methylation-specific PCR (MSP) showed that the amount of unmethylated p53 increased, indicating that the anthocyanins can down-regulate the methylation of p53.
authors.affiliation: Research Center of Molecular Biology, Inner Mongolia Medical University, Hohhot 010059, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Qi
authors.affiliation: Research Center of Molecular Biology, Inner Mongolia Medical University, Hohhot 010059, China.
authors.forename: Shaowei
authors.initials: S
authors.lastname: Li
authors.affiliation: Research Center of Molecular Biology, Inner Mongolia Medical University, Hohhot 010059, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Research Center of Molecular Biology, Inner Mongolia Medical University, Hohhot 010059, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D029798:Blueberry Plants / Q000737:chemistry*; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D006801:Humans; D007624:KB Cells; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects*; D008745:Methylation / Q000187:drug effects; D009062:Mouth Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D010936:Plant Extracts / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53