title: A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches.
abstract: A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction.
authors.affiliation: Centre for Microbial Ecology and Genomics (CMEG), Department of Genetics, University of Pretoria, South Africa.
authors.forename: Eoin
authors.initials: E
authors.lastname: Gunnigle
authors.affiliation: Centre for Microbial Ecology and Genomics (CMEG), Department of Genetics, University of Pretoria, South Africa.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Ramond
authors.affiliation: Centre for Microbial Ecology and Genomics (CMEG), Department of Genetics, University of Pretoria, South Africa.
authors.forename: Aline
authors.initials: A
authors.lastname: Frossard
authors.affiliation: Gobabeb Research and Training Centre, Walvis Bay, Namibia.
authors.forename: Mary
authors.initials: M
authors.lastname: Seeley
authors.affiliation: Centre for Microbial Ecology and Genomics (CMEG), Department of Genetics, University of Pretoria, South Africa. Electronic address: don.cowan@up.ac.za.
authors.forename: Don
authors.initials: D
authors.lastname: Cowan
mesh_terms: D005591:Chemical Fractionation / Q000379:methods; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D056186:Metagenomics; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D040901:Proteomics; D012313:RNA / Q000032:analysis / Q000302:isolation & purification*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D011506:Proteins; D012987:Soil; D012313:RNA; D004247:DNA
keywords: Co-extraction; Metaproteomics; Nucleic acids; Protein; T-RFLP