title: [Relationship between Line 1 methylation and clinical data of non-small cell lung cancer].
abstract: OBJECTIVE
  To explore the impact of Line-1 methylation on clinical features of non-small cell lung cancer and its connection with smoking and other living habits.
  
  
  METHODS
  Pyrosequencing was used to determine the extent of Line-1 methylation in cancer and adjacent tissues derived from 197 patients with primary non-small cell lung cancer. Non-conditional logistic regression analysis was performed to correlate the level of Line-1 methylation with clinical features and living habits of the patients.
  
  
  RESULTS
  Line-1 methylation for cancer tissue and adjacent tissue has measured 68.20±11.63 and 78.90±2.09, respectively (P < 0.01), and has been associated with TNM staging, smoking history and histopathological types.
  
  
  CONCLUSIONS
  Lung cancer tissue Line-1 methylation level is closely related with clinical features and smoking. There is also a correlation between histopathological types of lung cancer and relative hypomethylation of Line-1.
authors.affiliation: Environmental Epigenetics Laboratory, Department of Environmental Medicine, Faculty of Public Health, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, P.R. China. Emailjinedu@zju.edu.cn.
authors.forename: Xinneng
authors.initials: X
authors.lastname: Liu
authors.forename: Yongtang
authors.initials: Y
authors.lastname: Jin
authors.forename: Peiwei
authors.initials: P
authors.lastname: Xu
authors.forename: Chunmei
authors.initials: C
authors.lastname: Chen
authors.forename: Cong
authors.initials: C
authors.lastname: Tan
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D019175:DNA Methylation; D005260:Female; D006801:Humans; D020084:Long Interspersed Nucleotide Elements; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged