title: Chromosome analysis in embryos from young patients with previous parity.
abstract: This study included 173 young couples of proven fertility who had previously undergone preimplantation genetic screening for chromosomes X and Y for family balancing. Several months later, when the outcome of the pregnancies was already known, the blastomeres from the corresponding embryos transferred were reanalysed by fluorescence in-situ hybridization (FISH) for chromosomes 13, 16, 18, 21, 22 with the aim of investigating correlation with embryo viability and the level of FISH sensitivity (embryos confirmed to be euploid). According to the results, informative in 152 couples, the proportion of euploid embryos was significantly lower in 53 nonpregnant women when compared with 99 women with term pregnancy (49% versus 75% respectively, P < 0.001). In addition, in 21 nonpregnant patients, all embryos transferred were found to be chromosomally abnormal. The level of FISH sensitivity was calculated in the group of term pregnancies where the number of euploid embryos was expected to exceed or match with the number of babies born. The resulting false-negative rate was 4.0% per patient and 1.9% per embryo. These findings confirmed the limited prediction power of embryo morphology on implantation but also the relevance of chromosomal abnormalities in causing embryo demise.
authors.affiliation: The Farah Hospital, Amman, Jordan.
authors.forename: Z
authors.initials: Z
authors.lastname: Kilani
authors.affiliation: Reproductive Medicine Unit, SISMeR, Bologna, Italy.
authors.forename: Mc
authors.initials: M
authors.lastname: Magli
authors.affiliation: The Farah Hospital, Amman, Jordan.
authors.forename: E
authors.initials: E
authors.lastname: Qaddomi
authors.affiliation: Reproductive Medicine Unit, SISMeR, Bologna, Italy.
authors.forename: Ap
authors.initials: A
authors.lastname: Ferraretti
authors.affiliation: The Farah Hospital, Amman, Jordan.
authors.forename: M
authors.initials: M
authors.lastname: Shaban
authors.affiliation: Reproductive Medicine Unit, SISMeR, Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Crippa
authors.affiliation: The Farah Hospital, Amman, Jordan.
authors.forename: L
authors.initials: L
authors.lastname: Haj Hassan
authors.affiliation: Reproductive Medicine Unit, Obstetric Hospital, 2nd Floor, University College Hospital Huntley Street, London WC1 6AU, UK.
authors.forename: F
authors.initials: F
authors.lastname: Shenfield
authors.affiliation: Reproductive Medicine Unit, SISMeR, Bologna, Italy. Electronic address: luca.gianaroli@sismer.it.
authors.forename: L
authors.initials: L
authors.lastname: Gianaroli
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D010298:Parity; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*; D027724:Reproductive Techniques, Assisted; D012732:Sex Determination Analysis / Q000379:methods*; D012743:Sex Preselection / Q000379:methods*
keywords: aneuploidy; chromosomes; embryo mosaicism; fluorescence in-situ hybridization; implantation; preimplantation genetic screening