title: Molecular characterization of heat shock protein 70 (HSP 70) promoter in Japanese flounder (Paralichthys olivaceus), and the association of Pohsp70 SNPs with heat-resistant trait.
abstract: Ambient temperature is one of the major abiotic environmental factors determining the main parameters of fish vital activity. HSP70 plays an essential role in heat response. In this investigation, the promoter and structure of Paralichthys olivaceus hsp70 (Pohsp70) gene was cloned and predicted. 2558 bp upstream regulatory region of Pohsp70 was annotated with four potential promoter elements and four putative binding sites of transcription factors heat shock elements (HSE, nGAAn) in the upstream of the transcription start site. In addition, one intron with 454 bp in the 5'-noncoding region was found. Quantitative Real Time PCR analysis indicated that the transcript level of Pohsp70 was raised markedly after 1 h by heat shocked. Furthermore, 25 SNPs were identified in Pohsp70 by resequencing, seven of which was associated with heat resistance. In addition, two of the seven SNPs, namely SNP14 and SNP16, were observed in strong linkage disequilibrium. The haplotype with association analysis showed TAGGAG haplotype was more represented in heat susceptible group while (DEL/T) GAATA haplotype was more frequent in heat resistant group. The heat resistant SNPs and haplotype could be candidate markers potentially serving for selective breeding programs of Japanese flounder aimed at improving anti-stress and production.
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 266003 Qingdao, Shandong, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qi
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 266003 Qingdao, Shandong, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 266003 Qingdao, Shandong, China.
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 266003 Qingdao, Shandong, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 266003 Qingdao, Shandong, China.
authors.forename: Wenji
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 266003 Qingdao, Shandong, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 266003 Qingdao, Shandong, China. Electronic address: qzhang@ouc.edu.cn.
authors.forename: Quanqi
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001947:Breeding / Q000379:methods; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005432:Flounder / Q000235:genetics*; D040461:Gene Components; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006358:Hot Temperature; D015810:Linkage Disequilibrium; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018840:HSP70 Heat-Shock Proteins
keywords: Heat-resistant traits; Japanese flounder; SNPs; hsp70