title: Pistachio (Pistacia vera L.) gum: a potent inhibitor of reactive oxygen species.
abstract: In the present study, in order to evaluate antioxidant and radical scavenging properties of Pistachio gum (P-Gum), different bioanalytical methods such as DPPH(•) scavenging activity, DMPD(•+) radical scavenging activity, total antioxidant activity determination by ferric thiocyanate, reducing ability Fe(3+)-Fe(2+) transformation, Cuprac and FRAP assays, O2(•-) scavenging by riboflavin-methionine-illuminate system and ferrous ions (Fe(2+)) chelating activities by 2,2'-bipyridyl reagent were performed separately. P-Gum inhibited 54.2% linoleic acid peroxidation at 10 µg/ml concentration. On the other hand, BHA, BHT, α-tocopherol and trolox, pure antioxidant compounds, indicated inhibition of 80.3%, 73.5%, 36.2% and 72.0% on peroxidation of linoleic acid emulsion at the same concentration, respectively. In addition, all of sample had an effective DPPH(•), DMPD(•+) and O2(•-) scavenging, Fe(3+) reducing power by Fe(3+)-Fe(2+) transformation and FRAP assay, Cu(2+) reducing ability by Cuprac method and Fe(2+) chelating activities.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Atatürk University , Erzurum , Turkey .
authors.forename: M Hilal
authors.initials: MH
authors.lastname: Sehitoglu
authors.forename: Hatice
authors.initials: H
authors.lastname: Han
authors.forename: Pınar
authors.initials: P
authors.lastname: Kalin
authors.forename: İlhami
authors.initials: İ
authors.lastname: Gülçin
authors.forename: Ali
authors.initials: A
authors.lastname: Ozkan
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D005609:Free Radicals / Q000737:chemistry*; D007501:Iron / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010881:Piperidones / Q000737:chemistry; D027927:Pistacia / Q000737:chemistry*; D053149:Plant Gums / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000737:chemistry*; D013861:Thiocyanates / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D010881:Piperidones; D053149:Plant Gums; D017382:Reactive Oxygen Species; D013861:Thiocyanates; C491664:dimethylpiperidone; C103724:ferric thiocyanate; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron
keywords: Antioxidant activity; Pistachio (Pistacia vera) gum; metal chelating; radical scavenging