title: In-situ administration of dendritic cells following argon-helium cryosurgery enhances specific antiglioma immunity in mice.
abstract: Dendritic cells (DCs) are highly specialized antigen-presenting cells that play a key role in the activation of naive T cells. With an aim to explore whether in-situ administration of DCs following argon-helium cryosurgery could enhance specific antiglioma immunity in mice, we evaluated the validity of this approach in a murine subcutaneous GL261 glioma model. C57BL/6 mice models bearing subcutaneous GL261 glioma were established and then divided into four groups, namely, no-treatment group (n=14), DC group (n=14), cryosurgery group (n=15), and cryosurgery+DC group (n=15). Compared with the other groups, cryosurgery combined with DCs injection reduced tumor sizes and significantly prolonged survival. In addition, the combined treatment resulted in significantly increasing percentages of CD3, CD3CD4 cells, the ratio of CD3CD4/CD3CD8, and the level of serum interleukin-12 10 days after treatments. Furthermore, in the combined treatment group, Th1 cells were significantly higher than those in the other groups, and the splenic cytotoxic T lymphocyte of mice showed significantly increasing specific cytotoxicity against GL261 cells. These results indicated that in addition to the destruction of tumor, cryosurgery combined with DCs injection enhanced systemic antitumor immunity, suggesting the potential usefulness of the combined treatment in the clinical management of gliomas.
authors.affiliation: aDepartment of Neurosurgery, Zhujiang Hospital bInstitute of Neurosurgery, Key Laboratory on Brain Function Repair and Regeneration of Guangdong, Southern Medical University, Guangzhou, China cDepartment of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Chunnan
authors.initials: C
authors.lastname: Lin
authors.forename: Qifu
authors.initials: Q
authors.lastname: Wang
authors.forename: Guohui
authors.initials: G
authors.lastname: Lu
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Yin
authors.forename: Xiaozheng
authors.initials: X
authors.lastname: He
authors.forename: Hongchao
authors.initials: H
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Pan
authors.forename: Shizhong
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001128:Argon; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003131:Combined Modality Therapy; D003452:Cryosurgery / Q000379:methods*; D003713:Dendritic Cells / Q000637:transplantation*; D004195:Disease Models, Animal; D005260:Female; D005910:Glioma / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D006371:Helium; D018664:Interleukin-12 / Q000097:blood; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D011897:Random Allocation; D013154:Spleen / Q000276:immunology / Q000473:pathology; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D018664:Interleukin-12; D006371:Helium; D001128:Argon