title: Comparison of a PCR serotyping assay, Check&Trace assay for Salmonella, and Luminex Salmonella serotyping assay for the characterization of Salmonella enterica identified from fresh and naturally contaminated cilantro.
abstract: Salmonella enterica isolated from fresh cilantro samples collected through the USDA/AMS Microbiological Data Program (MDP) were used to compare a PCR serotyping assay against the Check&Trace assay and the Luminex (BioPlex) Salmonella serotyping assay. The study was conducted to evaluate the effectiveness of the three methods for serotyping Salmonella from both enrichment broth cultures and pure Salmonella cultures. In this investigation, Salmonella spp. serotyping was conducted using 24 h enrichment broth cultures and pure Salmonella cultures from cilantro samples, with the PCR serotyping assay. Conversely, the Check&Trace and Luminex for Salmonella assays required pure cultures for Salmonella serotyping. The cilantro samples contained S. enterica serovar Montevideo, Newport, Saintpaul, and Tennessee, identified by the PCR serotyping assay and Check&Trace for Salmonella, but the Luminex assay only identified two of the four serotypes of the cilantro samples. The anticipated impact from this study is that the PCR serotyping assay provides a time- and cost-effective means for screening, identifying and serotyping Salmonella using DNA extracted from 24 h enrichment cilantro samples.
authors.affiliation: U.S. Food and Drug Administration, Laurel, MD 20708, United States. Electronic address: junia.jean-gillesbeaubrun@fda.hhs.gov.
authors.forename: J
authors.initials: J
authors.lastname: Jean-Gilles Beaubrun
authors.affiliation: U.S. Food and Drug Administration, Laurel, MD 20708, United States.
authors.forename: L
authors.initials: L
authors.lastname: Ewing
authors.affiliation: U.S. Food and Drug Administration, Laurel, MD 20708, United States.
authors.forename: K
authors.initials: K
authors.lastname: Jarvis
authors.affiliation: U.S. Food and Drug Administration, Laurel, MD 20708, United States.
authors.forename: K
authors.initials: K
authors.lastname: Dudley
authors.affiliation: U.S. Food and Drug Administration, Laurel, MD 20708, United States; Oak Ridge Institute for Science and Technology, Oak Ridge, TN, United States.
authors.forename: C
authors.initials: C
authors.lastname: Grim
authors.affiliation: U.S. Food and Drug Administration, Laurel, MD 20708, United States.
authors.forename: G
authors.initials: G
authors.lastname: Gopinath
authors.affiliation: U.S. Food and Drug Administration, Laurel, MD 20708, United States; UMCP JIFSAN Program, 5201 Paint Branch Pkwy Patapsco Building Suite 2134, University of Maryland, College Park 20742-6730, United States.
authors.forename: M-L
authors.initials: ML
authors.lastname: Flamer
authors.affiliation: U.S. Food and Drug Administration, Laurel, MD 20708, United States; Frostburg State University, 101 Braddock Rd, Frostburg, MD 21532, United States.
authors.forename: W
authors.initials: W
authors.lastname: Auguste
authors.affiliation: U.S. Food and Drug Administration, Laurel, MD 20708, United States; Richard Montgomery High School, 250 Richard Montgomery Dr, Rockville, MD 20852, United States.
authors.forename: A
authors.initials: A
authors.lastname: Jayaram
authors.affiliation: U.S. Food and Drug Administration, Laurel, MD 20708, United States; Howard University, 2400 Sixth St NW, Washington, DC 20059, United States.
authors.forename: J
authors.initials: J
authors.lastname: Elmore
authors.affiliation: USDA/AMS Microbiological Data Program (MDP), 8609 Sudley Rd., Suite 206, Manassas, VA 20110, United States.
authors.forename: M
authors.initials: M
authors.lastname: Lamont
authors.affiliation: ORA/DFS/FERN, Rockville, MD 20857, United States.
authors.forename: T
authors.initials: T
authors.lastname: McGrath
authors.affiliation: U.S. Food and Drug Administration, Laurel, MD 20708, United States.
authors.forename: D E
authors.initials: DE
authors.lastname: Hanes
mesh_terms: D028526:Coriandrum / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012703:Serotyping / Q000295:instrumentation / Q000379:methods*; D014675:Vegetables / Q000382:microbiology*
chemical_list: D011933:Reagent Kits, Diagnostic
keywords: Cilantro; Molecular serotyping; Salmonella spp.