title: Expression patterns of chitinase and chitosanase produced from Bacillus cereus in suppression of phytopathogen.
abstract: Bacillus cereus MP-310 was incubated on various culture media substrates as LB, colloidal chitin, chitosan powder, and chitosan beads to investigate the concurrent expression patterns of chitinase and chitosanase isozymes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Chitinase activity increased rapidly with a maximum level after 6 days of incubation in CM-chitin medium. Major bands of chitinase isozymes were strongly expressed on SDS-PAGE in LB medium (four bands) and in colloidal chitin medium (five bands) after 6 days after incubation, and in chitosan powder medium (one band) and in chitosan beads medium (five bands) after 12 days after incubation. A major band of chitosanase isozymes was strongly expressed on SDS-PAGE in chitosan powder medium (one band) and in chitosan beads medium (one band) after 12 days of incubation.
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Dong-Jun
authors.initials: DJ
authors.lastname: Seo
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Ji-Hae
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Yong-Su
authors.initials: YS
authors.lastname: Song
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Ro-Dong
authors.initials: RD
authors.lastname: Park
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: woojung@chonnam.ac.kr.
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Jung
mesh_terms: D001409:Bacillus cereus / Q000201:enzymology* / Q000254:growth & development; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis*; D048271:Chitosan / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006026:Glycoside Hydrolases / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D002686:Chitin; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase; D002688:Chitinases
keywords: Bacillus cereus; Chitinase activity; Chitosanase activity; Isozyme