title: Copper and selenium: auxiliary measure to control infection by Haemonchus contortus in lambs.
abstract: The aim of this study was to evaluate the effects of selenium and copper on oxidative stress and its performance in lambs experimentally infected with Haemonchus contortus. Twenty-eight five-months old lambs were experimentally infected by the oral route with 5000 third-stage infective larvae and allocated into four groups, i.e., untreated animals, animals treated intramuscularly with sodium selenite (0.2 mg kg(-1)), animals treated subcutaneously with copper (3.5 mg kg(-1)), and animals treated with sodium selenite (IM; 0.2 mg kg(-1)) and copper (SC; 3.5 mg kg(-1)). These animals received oat hay (Avena sativa) and commercial concentrate, totaling 15% of crude protein, 30% being derived from oat hay and 70% of the concentrate. Lipid peroxidation, antioxidant enzymes, eggs per gram of feces (EPG) and body weight were assessed on the day of infection and after 20, 40, 60 and 80 days post-infection. The number of H. contortus adults was assessed at the end of the experiment. The selenium associated or not with copper reduced the effects of oxidative stress caused by infection. The groups supplemented with copper had increased body weight, and the combination of these two minerals reduced the EPG and number of H. contortus adults in lambs. The use of selenium associated with copper may help the control of infection by H. contortus.
authors.affiliation: Laboratório de Endocrinologia e Metabologia Animal (LEMA), Universidade Federal de Santa Maria, Avenida Roraima, 1000, Bairro Camobi, 97105-900 Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Marta Lizandra do Rêgo
authors.initials: ML
authors.lastname: Leal
authors.affiliation: Laboratório de Endocrinologia e Metabologia Animal (LEMA), Universidade Federal de Santa Maria, Avenida Roraima, 1000, Bairro Camobi, 97105-900 Santa Maria, Rio Grande do Sul, Brazil. Electronic address: felipe.pivoto@gmail.com.
authors.forename: Felipe Lamberti
authors.initials: FL
authors.lastname: Pivoto
authors.affiliation: Laboratório de Endocrinologia e Metabologia Animal (LEMA), Universidade Federal de Santa Maria, Avenida Roraima, 1000, Bairro Camobi, 97105-900 Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Guilherme Costa
authors.initials: GC
authors.lastname: Fausto
authors.affiliation: Laboratório de Endocrinologia e Metabologia Animal (LEMA), Universidade Federal de Santa Maria, Avenida Roraima, 1000, Bairro Camobi, 97105-900 Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Adelina Rodrigues
authors.initials: AR
authors.lastname: Aires
authors.affiliation: Laboratório de Endocrinologia e Metabologia Animal (LEMA), Universidade Federal de Santa Maria, Avenida Roraima, 1000, Bairro Camobi, 97105-900 Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Thirssa Helena
authors.initials: TH
authors.lastname: Grando
authors.affiliation: Laboratório de Bioquímica Toxicologica, Universidade Federal de Santa Maria, Brazil.
authors.forename: Daniel Henrique
authors.initials: DH
authors.lastname: Roos
authors.affiliation: Laboratório de Bioquímica Toxicologica, Universidade Federal de Santa Maria, Brazil.
authors.forename: Jéssie Haigert
authors.initials: JH
authors.lastname: Sudati
authors.affiliation: Laboratório de Bioquímica Toxicologica, Universidade Federal de Santa Maria, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Wagner
authors.affiliation: Laboratório de Analises Clínica Veterinária (LACVET), Universidade Federal de Santa Maria, Brazil.
authors.forename: Márcio Machado
authors.initials: MM
authors.lastname: Costa
authors.affiliation: Laboratório de Doenças Parasitárias, Universidade Federal do Parana, Rua dos Funcionários, 1540, Bairro Juvevê, 80035-050 Curitiba, Paraná, Brazil.
authors.forename: Marcelo Beltrão
authors.initials: MB
authors.lastname: Molento
authors.affiliation: Laboratório de Bioquímica Toxicologica, Universidade Federal de Santa Maria, Brazil.
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: da Rocha
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D002374:Catalase / Q000097:blood; D003300:Copper / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D005243:Feces / Q000469:parasitology; D005979:Glutathione Peroxidase / Q000097:blood; D006188:Haemonchiasis / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D006400:Hematocrit / Q000662:veterinary; D007121:Immunocompetence / Q000187:drug effects; D007273:Injections, Intramuscular / Q000662:veterinary; D007279:Injections, Subcutaneous / Q000662:veterinary; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010270:Parasite Egg Count / Q000662:veterinary; D064586:Selenic Acid / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D015430:Weight Gain
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper; D002374:Catalase; D005979:Glutathione Peroxidase; D064586:Selenic Acid
keywords: Antioxidant; Gastrointestinal nematode; Oxidative stress; Trace element