title: Microfluidic Channels on Nanopatterned Substrates: Monitoring Protein Binding to Lipid Bilayers with Surface-Enhanced Raman Spectroscopy.
abstract: We used Surface Enhanced Raman Spectroscopy (SERS) to detect binding events between streptavidin and biotinylated lipid bilayers. The binding events took place at the surface between microfluidic channels and anodized aluminum oxide (AAO) with the latter serving as substrates. The bilayers were incorporated in the substrate pores. It was revealed that non-bound molecules were easily washed away and that large suspended cells (Salmonella enterica) are less likely to interfere with the monitoring process: when focusing to the lower surface of the channel, one may resolve mostly the bound molecules.
authors.affiliation: Electronic Imaging Center, New Jersey Institute of Technology, Newark, New Jersey, 07102.
authors.forename: Amrita
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: Electrical and Computer Engineering Department, New Jersey Institute of Technology, Newark, New Jersey, 07102.
authors.forename: R
authors.initials: R
authors.lastname: Perez-Castillejos
authors.affiliation: Department of Biology, Texas State University, San Marcos, Texas, 78666.
authors.forename: D
authors.initials: D
authors.lastname: Hahn
authors.affiliation: Department of Chemistry, North Carolina State University, 2620 Yarbrough Dr., Raleigh, North Carolina, 27695-8204.
authors.forename: Alex I
authors.initials: AI
authors.lastname: Smirnov
authors.affiliation: Electronic Imaging Center, New Jersey Institute of Technology, Newark, New Jersey, 07102.
authors.forename: H
authors.initials: H
authors.lastname: Grebel