title: Ability of horse anti-Tityus discrepans F(ab')2 ELISA assay to recognize Tityus discrepans venom toxins.
abstract: Anti-Tityus discrepans F(ab')2 ELISA recognition of T. discrepans toxins was measured with regression analysis and its slope called ELISA recognition value (ERv). Fractions containing toxins affecting mammal macrophages or Na(+)-channels have Ervs >19. Toxins affecting potassium channels or insect NaV channels have ERvs <10. Fractions including curarizing or antineoplasic peptides had ERvs <1. Erv increases in proportion to mammalian toxin toxicity rather than to toxin molecular mass.
authors.affiliation: Laboratory on Cellular Neuropharmacology, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Apdo. 20632, 1020A Caracas, Miranda, Venezuela.
authors.forename: P
authors.initials: P
authors.lastname: Díaz
authors.affiliation: Laboratory on Cellular Neuropharmacology, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Apdo. 20632, 1020A Caracas, Miranda, Venezuela. Electronic address: gdsuze@ivic.gob.ve.
authors.forename: G
authors.initials: G
authors.lastname: D'Suze
authors.affiliation: Laboratory on Cellular Neuropharmacology, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Apdo. 20632, 1020A Caracas, Miranda, Venezuela.
authors.forename: C
authors.initials: C
authors.lastname: Sevcik
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006736:Horses; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D008970:Molecular Weight; D012604:Scorpion Venoms / Q000032:analysis* / Q000276:immunology
chemical_list: D007140:Immunoglobulin Fab Fragments; D012604:Scorpion Venoms
keywords: Anti-Tityus F(ab')(2); ELISA recognition value; Scorpion venom; Tityus discrepans