title: Sedimentation field flow fractionation monitoring of in vitro enrichment in cancer stem cells by specific serum-free culture medium.
abstract: The development of methods to enrich cell populations for cancer stem cells (CSC) is urgently needed to help understand tumor progression, therapeutic escape and to evaluate new drugs, in particular for colorectal cancer (CRC). In this work, we describe the in vitro use of OncoMiD for colon, a CRC-specific primary cell culture medium, to enrich CRC cell lines in CSC. Sedimentation field flow fractionation (SdFFF) was used to monitor the evolution of subpopulations composition. In these models, medium induced a loss of adherence properties associated with a balance between proliferation and apoptosis rates and, more important, an increased expression of relevant CSC markers, leading to specific SdFFF elution profile changes.
authors.affiliation: Université de Limoges, Institut 145 GEIST, EA 3842 "Homéostasie cellulaire et pathologies", Faculté de Médecine, 2 rue du Dr Marcland, 87025 Limoges Cedex, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Mélin
authors.affiliation: Université de Limoges, Institut 145 GEIST, EA 3842 "Homéostasie cellulaire et pathologies", Faculté de Médecine, 2 rue du Dr Marcland, 87025 Limoges Cedex, France; CHU de Limoges, Service de chirurgie digestive générale et endocrinienne, 2 rue Martin Luther King, 87042 Limoges Cedex, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Perraud
authors.affiliation: Oncomedics, 1 Avenue d'Ester, 87069 Limoges, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Bounaix Morand du Puch
authors.affiliation: Oncomedics, 1 Avenue d'Ester, 87069 Limoges, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Loum
authors.affiliation: Oncomedics, 1 Avenue d'Ester, 87069 Limoges, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Giraud
authors.affiliation: Université de Limoges, Institut 145 GEIST, EA 3842 "Homéostasie cellulaire et pathologies", Faculté de Médecine, 2 rue du Dr Marcland, 87025 Limoges Cedex, France; Université de Limoges, Institut 145 GEIST, EA 3842 "Homéostasie cellulaire et pathologies", Faculté de Pharmacie, Laboratoire de Chimie Analytique et Bromatologie, 87025 Limoges Cedex, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Cardot
authors.affiliation: Université de Limoges, Institut 145 GEIST, EA 3842 "Homéostasie cellulaire et pathologies", Faculté de Médecine, 2 rue du Dr Marcland, 87025 Limoges Cedex, France.
authors.forename: Marie-Odile
authors.initials: MO
authors.lastname: Jauberteau
authors.affiliation: Oncomedics, 1 Avenue d'Ester, 87069 Limoges, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Lautrette
authors.affiliation: Université de Limoges, Institut 145 GEIST, EA 3842 "Homéostasie cellulaire et pathologies", Faculté de Médecine, 2 rue du Dr Marcland, 87025 Limoges Cedex, France; Université de Limoges, Institut 145 GEIST, EA 3842 "Homéostasie cellulaire et pathologies", Faculté de Pharmacie, Laboratoire de Chimie Analytique et Bromatologie, 87025 Limoges Cedex, France. Electronic address: serge.battu@unilim.fr.
authors.forename: Serge
authors.initials: S
authors.lastname: Battu
authors.affiliation: Université de Limoges, Institut 145 GEIST, EA 3842 "Homéostasie cellulaire et pathologies", Faculté de Médecine, 2 rue du Dr Marcland, 87025 Limoges Cedex, France; CHU de Limoges, Service de chirurgie digestive générale et endocrinienne, 2 rue Martin Luther King, 87042 Limoges Cedex, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Mathonnet
mesh_terms: D017209:Apoptosis; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002469:Cell Separation / Q000295:instrumentation*; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D003470:Culture Media / Q000378:metabolism*; D031521:Fractionation, Field Flow / Q000295:instrumentation*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000473:pathology
chemical_list: D003470:Culture Media
keywords: Cancer stem cells; Cell enrichment; Colorectal cancer; Defined medium; Sedimentation field flow fractionation