title: Polymorphic microsatellite loci isolated from Cervus unicolor (Cervidae) show inbreeding in a domesticated population of Taiwan Sambar deer.
abstract: Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci but one, CU18, deviated from Hardy-Weinberg equilibrium due to excessive homozygosity in these domesticated broodstocks, reflecting inbreeding. These microsatellite loci will be useful, not only for assessment of population structure and genetic variability, but also for conservation of wild deer populations in Taiwan.
authors.affiliation: Department of Life Sciences, Cheng Kung University, Tainan, Taiwan lin0429@mail.tlri.gov.tw.
authors.forename: D Y
authors.initials: DY
authors.lastname: Lin
authors.affiliation: Department of Life Sciences, Cheng Kung University, Tainan, Taiwan.
authors.forename: T Y
authors.initials: TY
authors.lastname: Chiang
authors.affiliation: Kinmen National Park, Jinning Shiang, Kinmen, Taiwan.
authors.forename: C C
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Department of Life Sciences, Cheng Kung University, Tainan, Taiwan.
authors.forename: H D
authors.initials: HD
authors.lastname: Lin
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Chung Hwa University of Medical Technology, Rende, Tainan, Taiwan.
authors.forename: S J
authors.initials: SJ
authors.lastname: Tzeng
authors.affiliation: Kaohsiung Animal Propagation Station, COA-LRI, Pingtung, Taiwan.
authors.forename: S R
authors.initials: SR
authors.lastname: Kang
authors.affiliation: Department of Life Sciences, Cheng Kung University, Tainan, Taiwan.
authors.forename: H M
authors.initials: HM
authors.lastname: Sung
authors.affiliation: Division of Breeding and Genetics, COA-LRI, Muchang, Xinhua, Tainan, Taiwan.
authors.forename: M C
authors.initials: MC
authors.lastname: Wu
mesh_terms: D000483:Alleles; D000818:Animals; D000829:Animals, Domestic / Q000235:genetics; D003670:Deer / Q000235:genetics*; D005828:Genetics, Population; D007178:Inbreeding; D018895:Microsatellite Repeats / Q000235:genetics*; D013624:Taiwan