title: Rapid molecular identification of human taeniid cestodes by pyrosequencing approach.
abstract: Taenia saginata, T. solium, and T. asiatica are causative agents of taeniasis in humans. The difficulty of morphological identification of human taeniids can lead to misdiagnosis or confusion. To overcome this problem, several molecular methods have been developed, but use of these tends to be time-consuming. Here, a rapid and high-throughput pyrosequencing approach was developed for the identification of three human taeniids originating from various countries. Primers targeting the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of the three Taenia species were designed. Variations in a 26-nucleotide target region were used for identification. The reproducibility and accuracy of the pyrosequencing technology was confirmed by Sanger sequencing. This technique will be a valuable tool to distinguish between sympatric human taeniids that occur in Thailand, Asia and Pacific countries. This method could potentially be used for the molecular identification of the taeniid species that might be associated with suspicious cysts and lesions, or cyst residues in humans or livestock at the slaughterhouse.
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand.
authors.forename: Tongjit
authors.initials: T
authors.lastname: Thanchomnang
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani, Thailand.
authors.forename: Chairat
authors.initials: C
authors.lastname: Tantrawatpan
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Pewpan M
authors.initials: PM
authors.lastname: Intapan
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Oranuch
authors.initials: O
authors.lastname: Sanpool
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Thasala, Nakhon Si Thammarat, Thailand.
authors.forename: Penchom
authors.initials: P
authors.lastname: Janwan
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Viraphong
authors.initials: V
authors.lastname: Lulitanond
authors.affiliation: Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand.
authors.forename: Somjintana
authors.initials: S
authors.lastname: Tourtip
authors.affiliation: Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasaki
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Wanchai
authors.initials: W
authors.lastname: Maleewong
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000138:chemical synthesis; D018509:DNA, Helminth / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015203:Reproducibility of Results; D013621:Taenia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D041202:Taenia saginata / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D041201:Taenia solium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV