title: Varicella zoster virus DNA does not accumulate in infected human neurons.
abstract: Varicella zoster virus (VZV) is an exclusively human neurotropic alphaherpesvirus. It is unclear why human neurons infected in vitro with VZV at low multiplicity of infection do not exhibit a cytopathic effect (CPE) even though all VZV genes are transcribed, VZV proteins from all kinetic classes are translated and minimal infectious virus is produced. Here, we show that the lack of VZV-induced CPE correlates with the low abundance of viral DNA.
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, Aurora, CO, USA.
authors.forename: Nicholas L
authors.initials: NL
authors.lastname: Baird
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, Aurora, CO, USA.
authors.forename: Jacqueline L
authors.initials: JL
authors.lastname: Bowlin
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, Aurora, CO, USA.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Yu
authors.affiliation: Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia; Center for Proteomics, Department of Histology and Embryology, University of Rijeka, Rijeka, Croatia.
authors.forename: Stipan
authors.initials: S
authors.lastname: Jonjić
authors.affiliation: Max von Pettenkofer Institut, Ludwig-Maximilians Universität München, München, Germany; Division of Pathway Medicine, University of Edinburgh, Edinburgh, United Kingdom.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Haas
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, Aurora, CO, USA; Department of Microbiology, University of Colorado School of Medicine, Aurora, CO, USA.
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Cohrs
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, Aurora, CO, USA; Department of Microbiology, University of Colorado School of Medicine, Aurora, CO, USA. Electronic address: don.gilden@ucdenver.edu.
authors.forename: Don
authors.initials: D
authors.lastname: Gilden
mesh_terms: D002460:Cell Line; D004279:DNA, Viral / Q000378:metabolism*; D014645:Herpesvirus 3, Human / Q000378:metabolism*; D006801:Humans; D009474:Neurons / Q000821:virology*; D010948:Viral Plaque Assay
chemical_list: D004279:DNA, Viral
keywords: Human neurons; Tissue culture; VZV DNA