title: Engineered 3D bioimplants using elastomeric scaffold, self-assembling peptide hydrogel, and adipose tissue-derived progenitor cells for cardiac regeneration.
abstract: Contractile restoration of myocardial scars remains a challenge with important clinical implications. Here, a combination of porous elastomeric membrane, peptide hydrogel, and subcutaneous adipose tissue-derived progenitor cells (subATDPCs) was designed and evaluated as a bioimplant for cardiac regeneration in a mouse model of myocardial infarction. SubATDPCs were doubly transduced with lentiviral vectors to express bioluminescent-fluorescent reporters driven by constitutively active, cardiac tissue-specific promoters. Cells were seeded into an engineered bioimplant consisting of a scaffold (polycaprolactone methacryloyloxyethyl ester) filled with a peptide hydrogel (PuraMatrix™), and transplanted to cover injured myocardium. Bioluminescence and fluorescence quantifications showed de novo and progressive increases in promoter expression in bioactive implant-treated animals. The bioactive implant was well adapted to the heart, and fully functional vessels traversed the myocardium-bioactive implant interface. Treatment translated into a detectable positive effect on cardiac function, as revealed by echocardiography. Thus, this novel implant is a promising construct for supporting myocardial regeneration.
authors.affiliation: Heart Failure and Cardiac Regeneration (ICREC) Research Program, Health Research Institute Germans Trias i Pujol (IGTP), Cardiology Service, Hospital Universitari Germans Trias i Pujol Badalona, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Soler-Botija
authors.affiliation: Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill Genetic Medicine Building, Chapel Hill NC, USA.
authors.forename: Juli R
authors.initials: JR
authors.lastname: Bagó
authors.affiliation: Heart Failure and Cardiac Regeneration (ICREC) Research Program, Health Research Institute Germans Trias i Pujol (IGTP), Cardiology Service, Hospital Universitari Germans Trias i Pujol Badalona, Spain.
authors.forename: Aida
authors.initials: A
authors.lastname: Llucià-Valldeperas
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València Valencia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Vallés-Lluch
authors.affiliation: Department of Bioengineering, IQS-School of Engineering, Ramon Llull University Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Castells-Sala
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València Valencia, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Martínez-Ramos
authors.affiliation: Department of Bioengineering, IQS-School of Engineering, Ramon Llull University Barcelona, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Fernández-Muiños
authors.affiliation: Department of Cardiovascular Surgery, Pompidou Hospital Paris, France.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Chachques
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València Valencia, Spain.
authors.forename: Manuel Monleón
authors.initials: MM
authors.lastname: Pradas
authors.affiliation: Department of Bioengineering, IQS-School of Engineering, Ramon Llull University Barcelona, Spain.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Semino
authors.affiliation: Heart Failure and Cardiac Regeneration (ICREC) Research Program, Health Research Institute Germans Trias i Pujol (IGTP), Cardiology Service, Hospital Universitari Germans Trias i Pujol Badalona, Spain ; Department of Medicine, Autonomous University Barcelona Spain.
authors.forename: Antoni
authors.initials: A
authors.lastname: Bayes-Genis
keywords: Cardiac regeneration; RECATABI; elastomeric membrane; self-assembling peptide hydrogel; subcutaneous ATDPCs