title: Active vitamin D possesses beneficial effects on the interaction between muscle and bone.
abstract: Vitamin D deficiency and advanced glycation end products (AGEs) are suggested to be involved in the pathogenesis of osteoporosis and sarcopenia. However, the effects of vitamin D and AGEs on myogenesis and the interaction between muscle and bone remains still unclear. We previously showed that osteoglycin (OGN) is secreted from myoblasts and stimulates osteoblastic differentiation, suggesting that it plays important roles in the interaction between muscle and bone. The aim of this study is thus to examine the effects of vitamin D and AGEs on myoblastic differentiation of C2C12 cells and osteoblastic differentiation of osteoblastic MC3T3-E1 cells through OGN expression. 1α,25-dihydroxyvitamin D3 (1,25D) and eldecalcitol, an active vitamin D analog, induced the expression of MyoD, myogenin and OGN, and these effects were abolished by vitamin D receptor (VDR) suppression by siRNA in C2C12 cells. Moreover, conditioned medium from 1,25D-pretreated C2C12 cells stimulated the expression of type 1 collagen and alkaline phosphatase in MC3T3-E1 cells, compared to control medium from 1,25D-untreated C2C12 cells. In contrast, conditioned medium from VDR-suppressed and 1,25D-pretreated C2C12 cells showed no effects. AGE2 and AGE3 suppressed the expression of MyoD, myogenin and OGN in C2C12 cells. Moreover, 1,25D blunted the AGEs' effects. In conclusion, these findings showed for the first time that active vitamin D plays important roles in myogenesis and muscle-induced osteoblastogenesis through OGN expression. Active vitamin D treatment may rescue the AGEs-induced sarcopenia as well as - suppressed osteoblastic differentiation via OGN expression in myoblasts.
authors.affiliation: Internal Medicine 1, Shimane University Faculty of Medicine, Izumo, Japan. Electronic address: ken1nai@med.shimane-u.ac.jp.
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Internal Medicine 1, Shimane University Faculty of Medicine, Izumo, Japan. Electronic address: ippei.k@med.shimane-u.ac.jp.
authors.forename: Ippei
authors.initials: I
authors.lastname: Kanazawa
authors.affiliation: Internal Medicine 1, Shimane University Faculty of Medicine, Izumo, Japan. Electronic address: yamaguch@med.shimane-u.ac.jp.
authors.forename: Toru
authors.initials: T
authors.lastname: Yamaguchi
authors.affiliation: Department of Laboratory Medicine, Shimane University Faculty of Medicine, Izumo, Japan. Electronic address: syano@med.shimane-u.ac.jp.
authors.forename: Shozo
authors.initials: S
authors.lastname: Yano
authors.affiliation: Department of Physiology and Regenerative Medicine, Kinki University Faculty of Medicine, Osakasayama, Japan. Electronic address: hkaji@med.kindai.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kaji
authors.affiliation: Internal Medicine 1, Shimane University Faculty of Medicine, Izumo, Japan. Electronic address: sugimoto@med.shimane-u.ac.jp.
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Sugimoto
mesh_terms: D016475:3T3 Cells; D000818:Animals; D050071:Bone Density Conservation Agents / Q000008:administration & dosage; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D017127:Glycation End Products, Advanced / Q000008:administration & dosage*; D051379:Mice; D032342:Muscle Cells; D024510:Muscle Development / Q000187:drug effects / Q000502:physiology; D032446:Myoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D014807:Vitamin D / Q000008:administration & dosage*
chemical_list: D050071:Bone Density Conservation Agents; D017127:Glycation End Products, Advanced; D014807:Vitamin D
keywords: Advanced glycation end products; Eldecalcitol; Muscle; Osteoglycin; Vitamin D