title: Gastric emptying is involved in Lactobacillus colonisation in mouse stomach.
abstract: Lactobacilli are indigenous microbes of the stomach of rodents, with much lower numbers being present in mice fed a purified diet than in those fed a non-purified diet. We postulated that gastric emptying (GE) is responsible for the different colonisation levels of lactobacilli and tested this hypothesis in the present study. BALB/cCr Slc mice were fed either a non-purified diet or a purified diet for 2 weeks. The number of gastric tissue-associated lactobacilli was lower in mice fed the purified diet than in those fed the non-purified diet. GE, estimated by measuring the food recovered from the stomach, was higher in mice fed the purified diet than in those fed the non-purified diet and correlated negatively with the number of lactobacilli. Mice fed the non-purified diet exhibited lower GE rates even when lactobacilli were eliminated by ampicillin administration through the drinking-water, suggesting that GE is the cause but not the consequence of different Lactobacillus colonisation levels. The plasma concentrations of acylated ghrelin, a gastric hormone that promotes GE, were higher in mice fed the purified diet than in those fed the non-purified diet. There was a negative correlation between GE and the number of lactobacilli in mice fed the non-purified diet, the purified diet, and the purified diet supplemented with sugarbeet fibre (200 g/kg diet) or carboxymethyl cellulose (40 g/kg diet). We propose that a higher GE rate contributes, at least in part, to lower gastric colonisation levels of lactobacilli in mice fed a purified diet.
authors.affiliation: Graduate School of Agriculture, Hokkaido University,Sapporo060-8589,Japan.
authors.forename: Yuraporn
authors.initials: Y
authors.lastname: Sahasakul
authors.affiliation: Graduate School of Life Science, Hokkaido University,Sapporo060-8589,Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Takemura
authors.affiliation: Laboratory of Food Biochemistry, Research Faculty of Agriculture, Hokkaido University,Kita-9, Nishi-9, Kita-ku,Sapporo060-8589,Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Sonoyama
mesh_terms: D000667:Ampicillin / Q000008:administration & dosage; D000818:Animals; D058491:Bacterial Load; D004032:Diet; D005260:Female; D005746:Gastric Emptying / Q000502:physiology*; D054439:Ghrelin / Q000097:blood / Q000235:genetics; D007778:Lactobacillus / Q000254:growth & development*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis; D013047:Specific Pathogen-Free Organisms; D013270:Stomach / Q000382:microbiology*
chemical_list: D054439:Ghrelin; D012333:RNA, Messenger; D000667:Ampicillin