title: Point-of-care multiplexed assays of nucleic acids using microcapillary-based loop-mediated isothermal amplification.
abstract: This report demonstrates a straightforward, robust, multiplexed and point-of-care microcapillary-based loop-mediated isothermal amplification (cLAMP) for assaying nucleic acids. This assay integrates capillaries (glass or plastic) to introduce and house sample/reagents, segments of water droplets to prevent contamination, pocket warmers to provide heat, and a hand-held flashlight for a visual readout of the fluorescent signal. The cLAMP system allows the simultaneous detection of two RNA targets of human immunodeficiency virus (HIV) from multiple plasma samples, and achieves a high sensitivity of two copies of standard plasmid. As few nucleic acid detection methods can be wholly independent of external power supply and equipment, our cLAMP holds great promise for point-of-care applications in resource-poor settings.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.forename: Yulei
authors.initials: Y
authors.lastname: Liu
authors.forename: Xingjie
authors.initials: X
authors.lastname: Ma
authors.forename: Shangjin
authors.initials: S
authors.lastname: Cui
authors.forename: Liying
authors.initials: L
authors.lastname: Ma
authors.forename: Jianzhong Jeff
authors.initials: JJ
authors.lastname: Xi
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D004560:Electricity; D004867:Equipment Design; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D015658:HIV Infections / Q000175:diagnosis / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D012367:RNA, Viral; C007740:fluorexon