title: Multiple interactions of the intrinsically disordered region between the helicase and nuclease domains of the archaeal Hef protein.
abstract: Hef is an archaeal protein that probably functions mainly in stalled replication fork repair. The presence of an unstructured region was predicted between the two distinct domains of the Hef protein. We analyzed the interdomain region of Thermococcus kodakarensis Hef and demonstrated its disordered structure by CD, NMR, and high speed atomic force microscopy (AFM). To investigate the functions of this intrinsically disordered region (IDR), we screened for proteins interacting with the IDR of Hef by a yeast two-hybrid method, and 10 candidate proteins were obtained. We found that PCNA1 and a RecJ-like protein specifically bind to the IDR in vitro. These results suggested that the Hef protein interacts with several different proteins that work together in the pathways downstream from stalled replication fork repair by converting the IDR structure depending on the partner protein.
authors.affiliation: From the Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, and Faculty of Agriculture, Kyushu University, Fukuoka 812-8581.
authors.forename: Sonoko
authors.initials: S
authors.lastname: Ishino
authors.affiliation: From the Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, and Faculty of Agriculture, Kyushu University, Fukuoka 812-8581.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamagami
authors.affiliation: From the Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, and Faculty of Agriculture, Kyushu University, Fukuoka 812-8581.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kitamura
authors.affiliation: the Bio-AFM Frontier Research Center and Department of Physics, College of Science and Engineering, Kanazawa University, Kanazawa 920-1192, and.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kodera
authors.affiliation: the Bio-AFM Frontier Research Center and Department of Physics, College of Science and Engineering, Kanazawa University, Kanazawa 920-1192, and.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mori
authors.affiliation: the Bio-AFM Frontier Research Center and Department of Physics, College of Science and Engineering, Kanazawa University, Kanazawa 920-1192, and.
authors.forename: Shyogo
authors.initials: S
authors.lastname: Sugiyama
authors.affiliation: the Bio-AFM Frontier Research Center and Department of Physics, College of Science and Engineering, Kanazawa University, Kanazawa 920-1192, and.
authors.forename: Toshio
authors.initials: T
authors.lastname: Ando
authors.affiliation: the Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya 464-8601, Japan.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Goda
authors.affiliation: the Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya 464-8601, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tenno
authors.affiliation: the Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya 464-8601, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Hiroaki
authors.affiliation: From the Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, and Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, ishino@agr.kyushu-u.ac.jp.
authors.forename: Yoshizumi
authors.initials: Y
authors.lastname: Ishino
mesh_terms: D019843:Archaeal Proteins / Q000378:metabolism*; D001483:Base Sequence; D002942:Circular Dichroism; D004265:DNA Helicases / Q000378:metabolism*; D017931:DNA Primers; D004260:DNA Repair; D004720:Endonucleases / Q000378:metabolism*; D064267:Intrinsically Disordered Proteins / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D019906:Nuclear Magnetic Resonance, Biomolecular; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D019712:Thermococcus / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D019843:Archaeal Proteins; D017931:DNA Primers; D064267:Intrinsically Disordered Proteins; D004720:Endonucleases; D004265:DNA Helicases
keywords: Archaea; DNA Repair; DNA Replication; Extremophiles; Fancni Anemia; Intrinsically Disordered Protein; Protein-Protein Interaction; Stalled Replication Fork