title: F-box protein RcyA controls turnover of the kinesin-7 motor KipA in Aspergillus nidulans.
abstract: Fungal filamentous growth depends on continuous membrane insertion at the tip, the delivery of membrane-bound positional markers, and the secretion of enzymes for cell wall biosynthesis. This is achieved through exocytosis. At the same time, polarized growth requires membrane and protein recycling through endocytosis. Endocytic vesicles are thought to enter the protein degradation pathway or recycle their content to the cell surface. In Saccharomyces cerevisiae, the Rcy1 F-box protein is involved in the recycling process of a v-SNARE protein. We identified a Rcy1 orthologue, RcyA, in the filamentous fungus Aspergillus nidulans as a protein interacting with the KipA kinesin-7 motor protein in a yeast two-hybrid screen. The interaction was confirmed through bimolecular fluorescence complementation. RcyA possesses an F-box domain at the N terminus and a prenylation (CaaX) motif at the C terminus. RcyA shows also similarity to Sec10, a component of the exocyst complex. The RcyA protein localized to the hyphal tip and forming septa, likely through transportation on secretory vesicles and partially on early endosomes, but independently of KipA. Deletion of rcyA did not cause severe morphological changes but caused partial defects in the recycling of the SynA v-SNARE protein and the positioning of the cell end markers TeaA and TeaR. In addition, deletion of rcyA led to increased concentrations of the KipA protein, whereas the transcript concentration was unaffected. These results suggest that RcyA probably labels KipA for degradation and thereby controls the protein amount of KipA.
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Applied Biosciences, Dept. of Microbiology, Karlsruhe, Germany.
authors.forename: Saturnino
authors.initials: S
authors.lastname: Herrero
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Applied Biosciences, Dept. of Microbiology, Karlsruhe, Germany University of Tsukuba, Faculty of Life and Environmental Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Takeshita
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Applied Biosciences, Dept. of Microbiology, Karlsruhe, Germany reinhard.fischer@KIT.edu.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Fischer
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology*; D044783:F-Box Proteins / Q000502:physiology*; D015870:Gene Expression; D016547:Kinesins / Q000378:metabolism*; D021381:Protein Transport; D059748:Proteolysis; D020798:Two-Hybrid System Techniques; D054875:Ubiquitination
chemical_list: D044783:F-Box Proteins; D016547:Kinesins