title: [Genetic characterization of echovirus 6 isolated from meningitis and encephalitis cases in Shandong Province, China].
abstract: To analyze the genetic characteristics of echovirus 6 (E6) isolated from meningitis and encephalitis cases in Shandong Province, China, we collected cerebrospinal fluid samples from meningitis and encephalitis cases in Shandong Province from 2007 to 2012 for virus isolation. Viral RNAs were extracted from positive isolates, and complete VP1 coding regions were amplified by RT-PCR and sequenced. Homology comparison and phylogenetic analysis were performed. Six isolates were identified as E6 by microneutralization assay and molecular typing. The homology analysis showed that the six isolates had 78. 6%-99. 8% nucleotide and 95. 5%-100. 0% amino acid identities with each other, as well as 76. 9%-78. 4% nucleotide and 92. 3%-95. 1% amino acid identities with the prototype strain (D' Amori). The phylogenetic analysis based on the integrated VP1 sequences indicated that all Shandong E6 isolates could be separated into four clusters, designated as A, B, C, and D. The six E6 isolates belonged to clusters A, B, and D. Our study reveals high genetic differences between Shandong E6 isolates and suggests different transmission lineages of E6 co-circulated in Shandong Province.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Lin
authors.forename: Ze-Xin
authors.initials: ZX
authors.lastname: Tao
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Song
authors.forename: Su-Ting
authors.initials: ST
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Ji
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Ai-Qiang
authors.initials: AQ
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004458:Echovirus 6, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004660:Encephalitis / Q000453:epidemiology / Q000821:virology*; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007223:Infant; D008297:Male; D008581:Meningitis / Q000453:epidemiology / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins