title: Omics-based interpretation of synergism in a soil-derived cellulose-degrading microbial community.
abstract: Reaching a comprehensive understanding of how nature solves the problem of degrading recalcitrant biomass may eventually allow development of more efficient biorefining processes. Here we interpret genomic and proteomic information generated from a cellulolytic microbial consortium (termed F1RT) enriched from soil. Analyses of reconstructed bacterial draft genomes from all seven uncultured phylotypes in F1RT indicate that its constituent microbes cooperate in both cellulose-degrading and other important metabolic processes. Support for cellulolytic inter-species cooperation came from the discovery of F1RT microbes that encode and express complimentary enzymatic inventories that include both extracellular cellulosomes and secreted free-enzyme systems. Metabolic reconstruction of the seven F1RT phylotypes predicted a wider genomic rationale as to how this particular community functions as well as possible reasons as to why biomass conversion in nature relies on a structured and cooperative microbial community.
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA [3].
authors.forename: Yizhuang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: 1] Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, 1432 NORWAY [2].
authors.forename: Phillip B
authors.initials: PB
authors.lastname: Pope
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Shaochun
authors.initials: S
authors.lastname: Li
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Bo
authors.initials: B
authors.lastname: Wen
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Fengji
authors.initials: F
authors.lastname: Tan
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Shu
authors.initials: S
authors.lastname: Cheng
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Yang
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Lei
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Su
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Chengran
authors.initials: C
authors.lastname: Zhou
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Microbial Resources, The Institute of Microbiology, Chinese Academy of Sciences. Beijing 100101, CHINA.
authors.forename: Xiuzhu
authors.initials: X
authors.lastname: Dong
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Tao
authors.initials: T
authors.lastname: Jin
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yang
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Gengyun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Huangming
authors.initials: H
authors.lastname: Yang
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA [3] State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, CHINA.
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, 1432 NORWAY.
authors.forename: Vincent G H
authors.initials: VG
authors.lastname: Eijsink
authors.affiliation: 1] BGI-Shenzhen, Shenzhen 518083, CHINA [2] Department of Biology, University of Copenhagen, Copenhagen, DENMARK [3] King Abdulaziz University, Jeddah, SAUDI ARABIA [4] Macau University of Science and Technology, Macau, CHINA.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018533:Biomass; D002482:Cellulose / Q000378:metabolism*; D044603:Cellulosomes / Q000235:genetics / Q000378:metabolism; D016000:Cluster Analysis; D023281:Genomics / Q000379:methods*; D059013:Microbial Consortia; D010802:Phylogeny; D040901:Proteomics / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D002482:Cellulose