title: Isolation and identification of an endophytic fungus Pezicula sp. in Forsythia viridissima and its secondary metabolites.
abstract: In a survey of endophytic fungal biodiversity, an antimicrobial endophytic isolate zjwcf069 was obtained from twigs of Forsythia viridissima, Zhejiang Province, Southeast China. Zjwcf069 was then identified as Pezicula sp. through combination of morphological and phylogenetic analysis based on ITS-rDNA. Zjwcf069 here represented the first endophytic fungus in Pezicula isolated from host F. viridissima. From the fermentation broth, four compounds were obtained through silica gel column chromatography and Sephadex LH-20 under the guide of bioassay. Their structures were elucidated by spectroscopic analysis as mellein (1), ramulosin (2), butanedioic acid (3), and 4-methoxy-1(3H)-isobenzofuranone (4). Compound 4 here stood for the very first time as natural product from microbes. In vitro antifungal assay showed that compound 1 displayed growth inhibition against 9 plant pathogenic fungi, especially Botrytis cinerea and Fulvia fulva with EC50 values below 50 μg/mL. Endophytic fungi in medicinal plants were good resources for bioactive secondary metabolites.
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Jiaying
authors.initials: J
authors.lastname: Wang
authors.forename: Guoping
authors.initials: G
authors.lastname: Wang
authors.forename: Yalei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Biqiang
authors.initials: B
authors.lastname: Zheng
authors.forename: Chulong
authors.initials: C
authors.lastname: Zhang
authors.forename: Liwei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001203:Ascomycota / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002681:China; D060026:Endophytes / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification; D019143:Evolution, Molecular; D005285:Fermentation; D031657:Forsythia / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009793:Ochratoxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010802:Phylogeny; D064210:Secondary Metabolism; D017422:Sequence Analysis, DNA; D019802:Succinic Acid / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D001688:Biological Products; D009793:Ochratoxins; D019802:Succinic Acid; C120538:ochracin