title: In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide.
abstract: Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.
authors.affiliation: Centre for Fine Particles Processing and Nanotechnologies, Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Belgrade, Serbia.
authors.forename: Nenad L
authors.initials: NL
authors.lastname: Ignjatović
authors.affiliation: Faculty of Dentistry, Department of Biomaterials, University of Oslo, Oslo, Norway.
authors.forename: Petar
authors.initials: P
authors.lastname: Ninkov
authors.affiliation: Faculty of Dentistry, Department of Biomaterials, University of Oslo, Oslo, Norway.
authors.forename: Roya
authors.initials: R
authors.lastname: Sabetrasekh
authors.affiliation: Faculty of Dentistry, Department of Biomaterials, University of Oslo, Oslo, Norway.
authors.forename: Staale Petter
authors.initials: SP
authors.lastname: Lyngstadaas
authors.affiliation: Centre for Fine Particles Processing and Nanotechnologies, Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Belgrade, Serbia.
authors.forename: Dragan P
authors.initials: DP
authors.lastname: Uskoković
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008911:Minocycline / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011098:Polyglactin 910 / Q000737:chemistry*; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects; D000078304:Tigecycline
chemical_list: D000900:Anti-Bacterial Agents; D002130:Calcium Phosphates; D003692:Delayed-Action Preparations; D011098:Polyglactin 910; D000078304:Tigecycline; C020243:calcium phosphate; D008911:Minocycline
keywords: Calcium-phosphate; multifunctional; nano drug delivery; poly-DL-lactide-co-glycolide