title: [Effect of Yishen Daluo Decoction on the expression of PLP, Olig1, and Olig2 in mice with experimental autoimmune encephalomyelitis].
abstract: OBJECTIVE
  To study the effect of Yishen Daluo Decoction (YDD) on the expression of protein lipoprotein (PLP), oligodendrocyte transcription factor 1 (Olig 1), and oligodendrocyte transcription factor 2 (Olig2) in mice with experimental autoimmune encephalomyelitis (EAE).
  
  
  METHODS
  Totally 40 mice were randomly divided into 4 groups, i.e., the normal group, the model group, the Chinese medicine (CM) group, and the Western medicine (WM) group, 10 mice in each group. Each mouse in the model, CM, and WM groups was subcutaneously injected with 200 microL antigen emulsion (containing 150 micro g PLP139 -151 and 400 micro g H37RA) in two parts at the upper abdomen on the first day. 100 microLBordetella pertussis juice (containing 0. 6 x 10(6) Bordetella pertussis) was injected by caudal vein on the first and the third day. On the 7th day after modeling, each mouse in the normal group and the model group was intragastrically given normal saline (0. 1 mL/10 g). YDD (0. 2 g crude drug/10 g) was intragastrically given to mice in the CM group, and prednisone (0. 039 mg/10 g) was intragastrically given to mice in the WM group. All mice were intervened for 54 days. Changes of PLP, Olig1, and Olig2 in the brain tissue of EAE mice were detected by Western blot. Results The levels of PLP and Olig2 in the brain tissue of the model group were less than those of the normal group (P <0.05). Compared with the model group, the levels of PLP, Olig1, and Olig2 in the brain tissue increased in the CM group (P <0.05); the levels of PLP and Olig2 in the brain tissue increased in the WM group (P <0.05). Compared with the WM group, the level of Olig1 in the brain tissue increased in the CM group (P <0.05).
  
  
  CONCLUSIONS
  YDD could enhance remyelination by elevating the levels of Olig1 and Olig2 in the brain tissue of EAE mice.
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Lou
authors.forename: Ling-qun
authors.initials: LQ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D001921:Brain; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D015870:Gene Expression; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D000076104:Oligodendrocyte Transcription Factor 2; D014157:Transcription Factors
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004365:Drugs, Chinese Herbal; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D014157:Transcription Factors; C494786:Olig1 protein, mouse; C512773:Olig2 protein, mouse; C525740:yishen