title: Covalent, sequence-specific attachment of long DNA molecules to a surface using DNA-templated click chemistry.
abstract: We present a novel method that covalently and sequence-specifically attaches long DNA molecules to a surface that is compatible with high-resolution atomic force microscopy (AFM) imaging. Surfaces prepared with this approach are ideally suited for performing biophysical experiments on single DNA molecules.
authors.affiliation: Chemistry and Chemical Biology, School of Natural Sciences, University of California, 5200 N. Lake Rd., Merced, CA 95343, USA. tao.ye@ucmerced.edu.
authors.forename: Gary R
authors.initials: GR
authors.lastname: Abel
authors.forename: Blessing Huynh
authors.initials: BH
authors.lastname: Cao
authors.forename: Jason E
authors.initials: JE
authors.lastname: Hein
authors.forename: Tao
authors.initials: T
authors.lastname: Ye
mesh_terms: D020029:Base Pairing; D057930:Click Chemistry / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D013499:Surface Properties
chemical_list: D003300:Copper; D004247:DNA