title: Genotoxicity and activation of cellular defenses in transplanted zebra mussels Dreissena polymorpha along the Seine river.
abstract: The aim of the present study was to confirm the relevance of studying DNA adduct formation in a field study. In that context, freshwater mussels Dreissena polymorpha, collected in a reference station, were transplanted in different sites with a pollution gradient. After one and two months, mussels were collected and DNA adduct formation was analyzed using the (32)P post labelling technique on both gills and digestive glands. In addition, the expression of genes involved in the detoxification system (catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), HSP70, aryl hydrocarbon receptor (AHR), P glycoprotein (PgP), metallothionein (MT)) was assessed by RT-PCR. DNA adducts were observed at amount comparable to data from literature. Increase of DNA adducts after two months of transplantation could be correlated with strong modulation of gene expression implicated in detoxification processes. Indeed, PgP and HSP70 gene expressions were similarly induced in gills and digestive glands while SOD and CAT expressions were down regulated in both tissues. AHR, GST and MT genes were differently regulated depending upon the tissue studied and the level of contamination in the different sites. We demonstrated that mussels transplanted in the different stations with pollution gradient were able to biotransform PAHs, assessed by DNA adduct formation and the high decrease of detoxification genes. Specific DNA adducts pattern obtained after one and two month mussel transplantations demonstrated the relevance of DNA adduct as biomarker of environmental pollution.
authors.affiliation: Cemagref, Unité de Recherche Hydrosystèmes et Bioprocédés, 1 rue Pierre-Gilles de Gennes, CS10030-92761 Antony, France. Electronic address: amelie.chatel@uco.fr.
authors.forename: Amélie
authors.initials: A
authors.lastname: Châtel
authors.affiliation: Université de Toulouse, INPT/ENSAT, Laboratoire de Génie Chimie, UMR-CNRS 5503, Département Bioprocédés et systèmes microbiens, 1 avenue agrobiopôle, 31320 Auzeville-Tolosane, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Faucet-Marquis
authors.affiliation: Cemagref, Unité de Recherche Hydrosystèmes et Bioprocédés, 1 rue Pierre-Gilles de Gennes, CS10030-92761 Antony, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Gourlay-Francé
authors.affiliation: Université de Toulouse, INPT/ENSAT, Laboratoire de Génie Chimie, UMR-CNRS 5503, Département Bioprocédés et systèmes microbiens, 1 avenue agrobiopôle, 31320 Auzeville-Tolosane, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Pfohl-Leszkowicz
authors.affiliation: Cemagref, Unité de Recherche Hydrosystèmes et Bioprocédés, 1 rue Pierre-Gilles de Gennes, CS10030-92761 Antony, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Vincent-Hubert
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D018736:DNA Adducts / Q000378:metabolism*; D004249:DNA Damage; D049875:Dreissena / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005880:Gills / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics; D045483:Rivers; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; D018336:Receptors, Aryl Hydrocarbon; D014874:Water Pollutants, Chemical; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
keywords: Biomarker; DNA adducts; Detoxification; Dreissena polymorpha; Pollution; RT-PCR