title: Synthesis and characterization of reduced graphene oxide supported gold nanoparticles-poly(pyrrole-co-pyrrolepropylic acid) nanocomposite-based electrochemical biosensor.
abstract: A conducting poly(pyrrole-co-pyrrolepropylic acid) copolymer nanocomposite film (AuNP-PPy-PPa) incorporating gold nanoparticles (AuNP) was electrochemically grown using a single step procedure over electrochemically reduced graphene oxide (RGO) flakes deposited on a silane-modified indium-tin-oxide (ITO) glass plate. The RGO support base provided excellent mechanical and chemical stability to the polymer nanocomposite matrix. The porous nanostructure of AuNP-PPy-PPa/RGO provided a huge accessible area to disperse AuNP, and it avoided metallic agglomeration within the polymer matrix. The AuNP-PPy-PPa/RGO was characterized by high-resolution transmission electron microscopy (HRTEM), contact angle measurements, Fourier transform infrared spectroscopy (FTIR), and electrochemical techniques. The pendant carboxyl group of AuNP-PPy-PPa/RGO was covalently bonded with myoglobin protein antibody, Ab-Mb, for the construction of a bioelectrode. Electrochemical impedance spectroscopy technique was used for the characterization of the bioelectrode and as an impedimetric biosensor for the detection of human cardiac biomarker, Ag-cMb. The bioelectrode exhibited a linear impedimetric response to Ag-cMb in the range of 10 ng mL(-1) to 1 μg mL(-1), in phosphate-buffered solution (PBS) (pH 7.4, 0.1 M KCl) with a sensitivity of 92.13 Ω cm(2) per decade.
authors.affiliation: CSIR-National Physical Laboratory, Dr. K.S. Krishnan Road, New Delhi, 110012, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Puri
authors.forename: Asad
authors.initials: A
authors.lastname: Niazi
authors.forename: Avanish K
authors.initials: AK
authors.lastname: Srivastava
authors.lastname: Rajesh
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques; D017097:Electric Impedance; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D009211:Myoglobin / Q000302:isolation & purification* / Q000378:metabolism; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D000906:Antibodies; D009211:Myoglobin; D011758:Pyrroles; D006046:Gold; D006108:Graphite