title: Genome-wide identification and functional prediction of novel and drought-responsive lincRNAs in Populus trichocarpa.
abstract: Protein-coding genes are considered to be a dominant component of the eukaryotic transcriptome; however, many studies have shown that intergenic, non-coding transcripts also play an important role. Long intergenic non-coding RNAs (lincRNAs) were found to play a vital role in human and Arabidopsis. However, lincRNAs and their regulatory roles remain poorly characterized in woody plants, especially Populus trichocarpa (P. trichocarpa). A large set of Populus RNA-Seq data were examined with high sequencing depth under control and drought conditions and a total of 2542 lincRNA candidates were identified. In total, 51 lincRNAs and 20 lincRNAs were identified as putative targets and target mimics of known Populus miRNAs, respectively. A total of 504 lincRNAs were found to be drought responsive, eight of which were confirmed by RT-qPCR. These findings provide a comprehensive view of Populus lincRNAs, which will enable in-depth functional analysis.
authors.affiliation: College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, mailbox 69, No. 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Shuai
authors.affiliation: College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, mailbox 69, No. 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liang
authors.affiliation: College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, mailbox 69, No. 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China.
authors.forename: Sha
authors.initials: S
authors.lastname: Tang
authors.affiliation: College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, mailbox 69, No. 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China.
authors.forename: Zhoujia
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, mailbox 69, No. 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China.
authors.forename: Chu-Yu
authors.initials: CY
authors.lastname: Ye
authors.affiliation: College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, mailbox 69, No. 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, mailbox 69, No. 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China xiaxl@bjfu.edu.cn yinwl@bjfu.edu.cn.
authors.forename: Xinli
authors.initials: X
authors.lastname: Xia
authors.affiliation: College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, mailbox 69, No. 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China xiaxl@bjfu.edu.cn yinwl@bjfu.edu.cn.
authors.forename: Weilun
authors.initials: W
authors.lastname: Yin
mesh_terms: D001483:Base Sequence; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics; D032107:Populus / Q000235:genetics* / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D062085:RNA, Long Noncoding
keywords: Drought; Populus trichocarpa; lincRNA; miRNA; non-coding RNA; target mimicry.