title: Identification and authentication of Rosa species through development of species-specific SCAR marker(s).
abstract: Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the influence of environmental factors, which is why a reliable fingerprinting method was developed to overcome this problem. The development of molecular markers will enable the identification of Rosa species. In the present study, randomly amplified polymorphic DNA (RAPD) analysis was done on four Rosa species, Rosa gruss-an-teplitz (Surkha), Rosa bourboniana, Rosa centifolia, and Rosa damascena. A polymorphic RAPD fragment of 391 bp was detected in R. bourboniana, which was cloned, purified, sequenced, and used to design a pair of species-specific sequence-characterized amplified region (SCAR) primers (forward and reverse). These SCAR primers were used to amplify the specific regions of the rose genome. These PCR amplifications with specific primers are less sensitive to reaction conditions, and due to their high reproducibility, these species-specific SCAR primers can be used for marker-assisted selection and identification of Rosa species.
authors.affiliation: Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan imranagrarian3@gmail.com.
authors.forename: K M I
authors.initials: KM
authors.lastname: Bashir
authors.affiliation: Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan.
authors.forename: F S
authors.initials: FS
authors.lastname: Awan
authors.affiliation: Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan.
authors.forename: I A
authors.initials: IA
authors.lastname: Khan
authors.affiliation: Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan.
authors.forename: A I
authors.initials: AI
authors.lastname: Khan
authors.affiliation: Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan.
authors.forename: M
authors.initials: M
authors.lastname: Usman
mesh_terms: D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D027826:Rosa / Q000235:genetics*; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018744:DNA, Plant