title: Meniscal repair in vivo using human chondrocyte-seeded PLGA mesh scaffold pretreated with platelet-rich plasma.
abstract: The objective of this study was to test the hypothesis that platelet-rich plasma (PRP) pretreatment on a poly-lactic-co-glycolic acid (PLGA) mesh scaffold enhances the healing capacity of the meniscus with human chondrocyte-seeded scaffolds in vivo, even when the seeded number of cells was reduced from 10 million to one million. A flexible PLGA mesh scaffold was pretreated with PRP using a centrifugal technique. One million human articular chondrocytes were seeded onto the scaffold by dynamic oscillation. After 7 days, scaffolds were placed between human meniscal discs and were implanted subcutaneously in nude mice for 6 weeks (n = 16/group). Fluorescence microscopy demonstrated uniform attachment of the chondrocytes throughout the scaffolds 24 h following seeding. Cell attachment analysis revealed a significantly increased number of chondrocytes on PRP-pretreated than non-treated scaffolds (p < 0.05). Field emission scanning electron microscopy revealed chondrocytes attached to the PRP-pretreated scaffolds interconnecting their cellular processes with the fibrin network at 24 h and day 7 of culture. Of the 16 constructs containing PRP-pretreated scaffolds implanted in mice, six menisci healed completely, nine healed incompletely and one did not heal. Histological results from the 16 control constructs containing non-treated scaffolds revealed that none had healed completely, four healed incompletely and 12 did not heal. The histological outcome between the groups was significantly different (p < 0.05). These findings suggest that human articular chondrocytes on PRP-pretreated PLGA mesh scaffolds demonstrate increased cell attachment and enhance the healing capacity of meniscus with a reduced number of seeding cells in a meniscal repair mouse model. Copyright © 2014 John Wiley & Sons, Ltd.
authors.affiliation: Department of Orthopaedic Surgery, Seoul National University College of Medicine, Korea.
authors.forename: Hong Suk
authors.initials: HS
authors.lastname: Kwak
authors.affiliation: Department of Orthopaedic Surgery, Seoul National University College of Medicine, Korea.
authors.forename: Jinwoo
authors.initials: J
authors.lastname: Nam
authors.affiliation: Department of Orthopaedic Surgery, Seoul National University College of Medicine, Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Orthopaedic Surgery, Seoul National University College of Medicine, Korea.|Medical Research Centre, Seoul National University, Korea.
authors.forename: Hee Joong
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Orthopaedic Surgery, Seoul National University College of Medicine, Korea.
authors.forename: Jeong Joon
authors.initials: JJ
authors.lastname: Yoo
mesh_terms: D000327:Adsorption; D000368:Aged; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002358:Cartilage, Articular / Q000166:cytology; D019902:Chondrocytes / Q000166:cytology*; D005260:Female; D005337:Fibrin / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D000072600:Meniscus / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D053657:Platelet-Rich Plasma; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014184:Transplantation, Homologous; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005337:Fibrin
keywords: PLGA scaffold; cell delivery; human chondrocyte; meniscal repair; platelet-rich plasma; tissue engineering