title: Establishment of NOD/SCID mouse model of central nervous system leukemia.
abstract: In the present study, we successfully established a NOD/SCID mouse model of central nervous system leukemia by injection of acute monocytic leukemia cell line SHI-1 cells into the lateral ventricle. Immunohistochemistry was used to detect human leukocyte common antigen in brain slices. Nested PCR assay was used to detect MLL/AF6 fusion gene expression. After injection, the condition of the mice gradually progressed to cachexia and death (median survival time, 25 days). Leukemic cells were identified in the lung, bone marrow, and lymph node of one mouse. Brain tissue sections showed invasion into the subdural space, pia mater, arachnoid, along the Virchow-Robin space and into the deep brain parenchyma. In summary, a central nervous system leukemia (CNSL) model was established in NOD/SCID mice.
authors.affiliation: Department of Pediatrics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, P.R. China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, P.R. China.
authors.forename: Pinggan
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, P.R. China.
authors.forename: Zhanwen
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Pediatrics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Meng
authors.affiliation: Department of Pediatrics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, P.R. China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Pediatrics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, P.R. China.
authors.forename: Jianpei
authors.initials: J
authors.lastname: Fang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016543:Central Nervous System Neoplasms / Q000401:mortality / Q000473:pathology*; D004195:Disease Models, Animal; D007942:Leukemia, Experimental / Q000473:pathology*; D007948:Leukemia, Monocytic, Acute / Q000473:pathology; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D051788:Myeloid-Lymphoid Leukemia Protein / Q000378:metabolism; D015514:Oncogene Proteins, Fusion / Q000378:metabolism; D016019:Survival Analysis
chemical_list: C447400:MLL-AF6 fusion protein, human; D015514:Oncogene Proteins, Fusion; D051788:Myeloid-Lymphoid Leukemia Protein