title: Toward a better follow-up of ovarian recovery in young women after chemotherapy with a hypersensitive antimüllerian hormone assay.
abstract: OBJECTIVE
  To evaluate the utility of a hypersensitive assay for measuring low antimüllerian hormone (AMH) levels in young cancer patients during the ovarian recovery phase of their chemotherapy.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  Academic medical center.
  
  
  METHODS
  Fifty-eight samples drawn at least 3 months after the end of chemotherapy in 30 women having either breast cancer (n=13) or hematologic malignancies (n=17) were selected to constitute two equally size groups: amenorrhea (n=30 samples) or spontaneous cycle (n=28 samples).
  
  
  METHODS
  None.
  
  
  METHODS
  Serum AMH levels were measured by a conventional AMH ELISA (EIA AMH/MIS) and a hypersensitive ELISA (PicoAMH, AnshLabs) on the same sample.
  
  
  RESULTS
  Using a conventional assay, serum AMH was detectable (≥3 pmol/L) in 6.7% and in 10.7% of the samples corresponding to amenorrheic or cycling patients, respectively (nonsignificant). By contrast, with PicoAMH, serum AMH was detectable (≥0.07 pmol/L) in 71.4% of the samples from cycling women vs. 16.7% of the samples from amenorrheic patients. Multivariate regression analysis showed that among putative contributors, only the menstrual status (r=0.307) and serum FSH level (r=-0.546) were independently correlated to a detectable serum AMH with the picoAMH assay exclusively.
  
  
  CONCLUSIONS
  The picoAMH assay, allowing measurement of very low AMH concentrations in human serum, should refine postchemotherapy ovarian follow-up in young women.
authors.affiliation: Service de Gynécologie Endocrinienne et Médecine de la Reproduction, Hôpital Jeanne de Flandre, Centre Hospitalier Régional Universitaire, Lille, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Decanter
authors.affiliation: Service de Gynécologie Endocrinienne et Médecine de la Reproduction, Hôpital Jeanne de Flandre, Centre Hospitalier Régional Universitaire, Lille, France.
authors.forename: Maeliss
authors.initials: M
authors.lastname: Peigne
authors.affiliation: Département de sénologie, Centre Oscar Lambret, Lille, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Mailliez
authors.affiliation: Service des maladies du sang, Hôpital Huriez, Centre Hospitalier Régional Universitaire, Lille, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Morschhauser
authors.affiliation: Laboratoire de Biochimie & Hormonologie, Centre de Biologie Pathologie, Centre Hospitalier Régional Universitaire, Lille, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Dassonneville
authors.affiliation: Service de Gynécologie Endocrinienne et Médecine de la Reproduction, Hôpital Jeanne de Flandre, Centre Hospitalier Régional Universitaire, Lille, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Dewailly
authors.affiliation: Laboratoire de Biochimie & Hormonologie, Centre de Biologie Pathologie, Centre Hospitalier Régional Universitaire, Lille, France. Electronic address: pascal.pigny@chru-lille.fr.
authors.forename: Pascal
authors.initials: P
authors.lastname: Pigny
mesh_terms: D000046:Academic Medical Centers; D000293:Adolescent; D000328:Adult; D000568:Amenorrhea / Q000097:blood / Q000139:chemically induced* / Q000503:physiopathology; D054304:Anti-Mullerian Hormone / Q000097:blood*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000009:adverse effects*; D015415:Biomarkers / Q000097:blood; D016009:Chi-Square Distribution; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D043373:Follicle Stimulating Hormone, Human / Q000097:blood; D006801:Humans; D008598:Menstruation; D015999:Multivariate Analysis; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D011237:Predictive Value of Tests; D020127:Recovery of Function; D012189:Retrospective Studies; D013997:Time Factors; D055815:Young Adult
chemical_list: D015415:Biomarkers; D043373:Follicle Stimulating Hormone, Human; D054304:Anti-Mullerian Hormone
keywords: AMH; cancer; chemotherapy; follow-up; ovarian follicles