title: Overexpression of histone deacetylases in cancer cells is controlled by interplay of transcription factors and epigenetic modulators.
abstract: Histone deacetylases (HDACs) that deacetylate histone and nonhistone proteins play crucial roles in a variety of cellular processes. The overexpression of HDACs is reported in many cancer types and is directly linked to accelerated cell proliferation and survival. However, little is known about how HDAC expression is regulated in cancer cells. In this study, we found that HDAC1 and HDAC2 promoters are regulated through collaborative binding of transcription factors Sp1/Sp3 and epigenetic modulators, including histone H3K4 methyltransferase SET1 and histone acetyltransferase p300, whose levels are also elevated in colon cancer cell lines and patient samples. Interestingly, Sp1 and Sp3 differentially regulate HDAC1 and HDAC2 promoter activity. In addition, Sp1/Sp3 recruits SET1 and p300 to the promoters. SET1 knockdown (KD) results in a loss of the H3K4 trimethylation mark at the promoters, as well as destabilizes p300 at the promoters. Conversely, p300 also influences SET1 recruitment and H3K4me3 level, indicating a crosstalk between p300 and SET1. Further, SET1 KD reduces Sp1 binding to the HDAC1 promoter through the increase of Sp1 acetylation. These results indicate that interactions among transcription factors and epigenetic modulators orchestrate the activation of HDAC1 and HDAC2 promoter activity in colon cancer cells.
authors.affiliation: Department of Anatomy and Cell Biology and.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA.
authors.forename: Tal
authors.initials: T
authors.lastname: Salz
authors.affiliation: Department of Anatomy and Cell Biology and.
authors.forename: Maria
authors.initials: M
authors.lastname: Zajac-Kaye
authors.affiliation: Department of Anatomy and Cell Biology and.
authors.forename: Daiqing
authors.initials: D
authors.lastname: Liao
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA qiuy@ufl.edu sumingh@ufl.edu.
authors.forename: Suming
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Anatomy and Cell Biology and qiuy@ufl.edu sumingh@ufl.edu.
authors.forename: Yi
authors.initials: Y
authors.lastname: Qiu
mesh_terms: D003110:Colonic Neoplasms / Q000378:metabolism*; D044127:Epigenesis, Genetic; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D019073:HT29 Cells; D056284:Histone Deacetylase 1 / Q000235:genetics / Q000378:metabolism*; D056464:Histone Deacetylase 2 / Q000235:genetics / Q000378:metabolism*; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D011401:Promoter Regions, Genetic; D050880:p300-CBP Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D011495:Histone-Lysine N-Methyltransferase; C526068:Setd1A protein, human; D050880:p300-CBP Transcription Factors; C110550:HDAC1 protein, human; C533423:HDAC2 protein, human; D056284:Histone Deacetylase 1; D056464:Histone Deacetylase 2
keywords: SET1; Sp1; Sp3; p300