title: Expression of claudin-3 in the esophagus and larynx of rat reflux model.
abstract: OBJECTIVE
  The aim of this study was to investigate the association between laryngeal expression of claudin-3 and laryngopharyngeal reflux (LPR) in a rat reflux model.
  
  
  METHODS
  Eight Wistar rats were divided into two groups. Four rats underwent total esophageal myectomy to induce reflux, and the remainder underwent a sham operation as a control. All animals were sacrificed 12 weeks after surgery to perform tissue histology and Western blot analysis.
  
  
  RESULTS
  Lymphocyte infiltration increased significantly in the study group in both esophageal and laryngeal samples (P=0.001, 0.002, respectively). Both esophageal and laryngeal expressions of claudin-3 were significantly lower in the study group when compared with that in the control group (P=0.045, 0.037, respectively).
  
  
  CONCLUSIONS
  The results of this study suggest that a decrease in claudin-3 could be a sensitive indicator of reflux laryngitis in rats.
authors.affiliation: Department of Otorhinolaryngology, EYE and ENT Hospital of Fudan University, China.
authors.forename: Xiao Bing
authors.initials: XB
authors.lastname: Xu
authors.affiliation: Department of Otorhinolaryngology, EYE and ENT Hospital of Fudan University, China. Electronic address: hu_ying_xxb_@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Research Center, EYE and ENT Hospital of Fudan University, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Research Center, EYE and ENT Hospital of Fudan University, China.
authors.forename: Chui Jin
authors.initials: CJ
authors.lastname: Lai
authors.affiliation: Department of Otorhinolaryngology, EYE and ENT Hospital of Fudan University, China.
authors.forename: Tian Yu
authors.initials: TY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D062465:Claudin-3 / Q000378:metabolism*; D004195:Disease Models, Animal; D004941:Esophagitis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D004947:Esophagus / Q000378:metabolism* / Q000473:pathology; D005764:Gastroesophageal Reflux / Q000150:complications / Q000378:metabolism / Q000473:pathology; D007827:Laryngitis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D057045:Laryngopharyngeal Reflux / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D007830:Larynx / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D062465:Claudin-3; C568124:Cldn3 protein, rat
keywords: Claudin-3; Gastroesophageal reflux disease; Laryngopharyngeal reflux