title: Immunomodulatory effects ofPotentilla indicaandDendrophthoe pentandraon mice splenocytes and thymocytes.
abstract: Immunomodulators are agents that are able to stimulate or inhibit the immune response. The leaf extracts fromPotentilla indicaandDendrophthoe pentandrawere analyzedin vitrofor immunomodulatory activity and an MTT colorimetric assay was conducted to determine the proliferation of mice splenocytes and thymocytes. A bromodeoxyuridine assay was performed to analyze DNA synthesis and the Trypan blue exclusion method was conducted to evaluate the changes in total cell population. The results indicated that treatment withP. indicaandD. pentandraproduced a time- and dose-dependent increase in cell viability and proliferation. Following 72 h of treatment withP. indicaandD. pentandra, thymocyte proliferation was augmented by 18 and 41%, respectively and splenocyte proliferation increased by 35 and 42%, respectively, when compared with untreated cells. The present study demonstrated that these extracts may act as potential immunostimulants and, thus, represent an alternative source of immunomodulatory compounds for the treatment of human immune-mediated diseases.
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia.
authors.forename: Hui Ying
authors.initials: HY
authors.lastname: Ang
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia.
authors.forename: Tamilselvan
authors.initials: T
authors.lastname: Subramani
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
authors.forename: Swee Keong
authors.initials: SK
authors.lastname: Yeap
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
authors.forename: Abdul Rahman
authors.initials: AR
authors.lastname: Omar
authors.affiliation: The University of Nottingham Malaysia Campus, Semenyih, Selangor 43500, Malaysia.
authors.forename: Wan Yang
authors.initials: WY
authors.lastname: Ho
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia.
authors.forename: Mohd Puad
authors.initials: MP
authors.lastname: Abdullah
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia.
authors.forename: Noorjahan Banu
authors.initials: NB
authors.lastname: Alitheen
keywords: MTT; Rosaceae; bromodeoxyuridine assay; immunomodulatory; mistletoe; spleen; thymus