title: [Characteristics of hydroxyapatite/PMMA nanocomposites for provisional restoration and its biocompatibility with human gingival fibroblasts].
abstract: OBJECTIVE
  To formulate hydroxyapatite (HA)/polymethyl methacrylate (PMMA) composites with improved cytocompatibility for provisional restoration.
  
  
  METHODS
  Nanocomposites with 20 wt%, 30 wt%, 40 wt%, and 50 wt% HA/PMMA (H/P) were developed and examined using X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). Human gingival fibroblasts were cultured on those HA/PMMA discs and investigated by fluorescent staining on 24 h and MTT assay at 1 d, 3 d, 5 d and 7 d.
  
  
  RESULTS
  Chemical integration of HA/PMMA interface was confirmed by XPS. Typical fusiform cells with adhesion spots were detected on 40 wt% and 50 wt% H/P discs. MTT results showed insignificant differences in cell growth between 40 wt% H/P and pure titanium (Ti, P > 0.05), while the other H/P discs showed significantly lower cell growth than pure Ti (P < 0.05).
  
  
  CONCLUSIONS
  40 wt% H/P might be a promising candidate for provisional dental implant restoration and for esthetic gingival contour.
authors.forename: Jing-chao
authors.initials: JC
authors.lastname: Zhang
authors.forename: An-chun
authors.initials: AC
authors.lastname: Mo
authors.forename: Ji-dong
authors.initials: JD
authors.lastname: Li
authors.forename: Xue-jiang
authors.initials: XJ
authors.lastname: Wang
authors.forename: Yu-bao
authors.initials: YB
authors.lastname: Li
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D017886:Durapatite; D005347:Fibroblasts; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D053761:Nanocomposites; D019904:Polymethyl Methacrylate; D014025:Titanium
chemical_list: D001672:Biocompatible Materials; D019904:Polymethyl Methacrylate; D017886:Durapatite; D014025:Titanium