title: New generation of bioorthogonally applicable fluorogenic dyes with visible excitations and large Stokes shifts.
abstract: Synthesis of a set of new, azide bearing, biorthogonally applicable fluorogenic dyes with large Stokes shifts is presented herein. To assess the fluorogenic performance of these new dyes we have labeled a genetically modulated, cyclooctyne-bearing protein in lysate medium. Studies showed that the labels produce specific signal with minimal background fluorescence. We also provide theoretical insights into the design of such fluorogenic labels.
authors.affiliation: "Lendület" Chemical Biology Research Group, Research Centre for Natural Sciences, Institute of Organic Chemistry, Hungarian Academy of Sciences , Magyar tudósok krt. 2., H-1117 Budapest, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Herner
authors.forename: Gemma
authors.initials: G
authors.lastname: Estrada Girona
authors.forename: Ivana
authors.initials: I
authors.lastname: Nikić
authors.forename: Mihály
authors.initials: M
authors.lastname: Kállay
authors.forename: Edward A
authors.initials: EA
authors.lastname: Lemke
authors.forename: Péter
authors.initials: P
authors.lastname: Kele
mesh_terms: D001386:Azides / Q000737:chemistry*; D034242:Cyclooctanes / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D058619:Maltose-Binding Proteins / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D014230:Triazoles / Q000737:chemistry*
chemical_list: D001386:Azides; D034242:Cyclooctanes; D005456:Fluorescent Dyes; D058619:Maltose-Binding Proteins; D014230:Triazoles