title: Development and bioevaluation of nanofibers with blood-derived growth factors for dermal wound healing.
abstract: The aim of our work was to produce a modern nanomaterial with incorporated blood-derived growth factors, produced by electrospinning, applicable in treatment of chronic wounds. Platelet-rich plasma was chosen as a natural source of growth factors. Results showed that platelet-rich plasma stimulates keratinocyte and fibroblast cell growth in vitro. Its optimal concentration in growth medium was 2% (v/v) for both types of skin cells, while higher concentrations caused alterations in cell morphology, with reduced cell mobility and proliferation. In the next step hydrophilic nanofibers loaded with platelet-rich plasma were produced from chitosan and poly(ethylene oxide), using electrospinning. The morphology of nanofibers was stable in aqueous conditions for 72 h. It was shown that electrospinning does not adversely affect the biological activity of platelet-rich plasma. The effects of nanofibers with incorporated platelet-rich plasma on cell proliferation, survival, morphology and mobility were examined. Nanofibers limited cell mobility, changed morphology and stimulated cell proliferation. Despite of the small amount of blood-derived growth factors introduced in cell culture via platelet-rich plasma-loaded nanofibers, such nanofibrillar support significantly induced cell proliferation, indicating synergistic effect of nanotopography and incorporated growth factors. The overall results confirm favorable in vitro properties of produced nanofibers, indicating their high potential as a nanomaterial suitable for delivery of platelet-rich plasma in wound healing applications.
authors.affiliation: University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia.
authors.forename: Valentina
authors.initials: V
authors.lastname: Bertoncelj
authors.affiliation: University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia.
authors.forename: Jan
authors.initials: J
authors.lastname: Pelipenko
authors.affiliation: University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia.
authors.forename: Julijana
authors.initials: J
authors.lastname: Kristl
authors.affiliation: University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia; Blood Transfusion Centre of Slovenia, Ljubljana, Slovenia; Celica d.o.o., Ljubljana, Slovenia.
authors.forename: Matjaž
authors.initials: M
authors.lastname: Jeras
authors.affiliation: Blood Transfusion Centre of Slovenia, Ljubljana, Slovenia.
authors.forename: Marko
authors.initials: M
authors.lastname: Cukjati
authors.affiliation: University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia. Electronic address: petra.kocbek@ffa.uni-lj.si.
authors.forename: Petra
authors.initials: P
authors.lastname: Kocbek
mesh_terms: D000279:Administration, Cutaneous; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000008:administration & dosage; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000008:administration & dosage; D011108:Polymers / Q000737:chemistry; D012867:Skin / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D011108:Polymers; D011092:Polyethylene Glycols; D048271:Chitosan
keywords: Electrospinning; Fibroblasts; Keratinocytes; Nanotechnology; Platelet-rich plasma; Polymer nanofibers; Wound healing