title: Proteomics of neuropathic pain: proteins and signaling pathways affected in a rat model.
abstract: The myriad proteins may be involved in the mechanisms underlying the development and maintenance of neuropathic pain, an extremely disabling condition that originates from pathology of the nervous system. To address the mechanisms, we here analyzed proteins and cellular networks in the dorsal spinal cord mediating pain processing in a well-established rat model of neuropathic pain induced by spinal nerve ligation (SNL). Labeling-based proteomic methods together with high-resolution mass spectrometry for proteome analysis were applied. 38 proteins including synapsin 1 and microtubule-associated protein 2 were identified as differently expressed in the SNL group. Pathway analysis suggests that maladaptive changes in the levels of these proteins may contribute to abnormal synaptic transmission and neuronal intracellular signaling underlying the onset and development of neuropathic pain.
authors.affiliation: Analytical Chemistry, Department of Chemistry - BMC and SciLifeLab and ‡Molecular Neuropsychopharmacology, Department of Pharmaceutical Biosciences, Uppsala University , P.O. Box 599, Husargatan 3, 75124 Uppsala, Sweden.
authors.forename: Ping
authors.initials: P
authors.lastname: Sui
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Watanabe
authors.forename: Michael H
authors.initials: MH
authors.lastname: Ossipov
authors.forename: Georgy
authors.initials: G
authors.lastname: Bakalkin
authors.forename: Konstantin
authors.initials: K
authors.lastname: Artemenko
authors.forename: Jonas
authors.initials: J
authors.lastname: Bergquist
mesh_terms: D000818:Animals; D006930:Hyperalgesia / Q000378:metabolism; D013058:Mass Spectrometry; D009437:Neuralgia / Q000378:metabolism*; D025341:Principal Component Analysis; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism*; D013127:Spinal Nerves / Q000293:injuries*
chemical_list: D020543:Proteome