title: Virus particle assembly into crystalline domains enabled by the coffee ring effect.
abstract: Tobacco mosaic virus particles can be rapidly assembled into 3D-domains by capillary flow-driven alignment at the triple contact-line of an evaporating droplet. Virus particles of ∼150 Å diameter can be resolved within individual domains at the outer rim of the "coffee-ring" type residue by atomic force microscopy. The crystalline domains can also be probed by X-ray microdiffraction techniques. Both techniques reveal that the rod-like virus particles are oriented parallel to the rim. We further demonstrate the feasibility of collection of hk0 reflection intensities in GISAXS geometry and show it allows calculating a low-resolution electron density projection along the rod axis.
authors.affiliation: European Synchrotron Radiation Facility, CS 40220, F-38043 Grenoble Cedex 9, France. riekel@esrf.fr.
authors.forename: Ronald
authors.initials: R
authors.lastname: Gebhardt
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Teulon
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Pellequer
authors.forename: Manfred
authors.initials: M
authors.lastname: Burghammer
authors.forename: Jacques-Philippe
authors.initials: JP
authors.lastname: Colletier
authors.forename: Christian
authors.initials: C
authors.lastname: Riekel
mesh_terms: D003460:Crystallization; D018625:Microscopy, Atomic Force; D014027:Tobacco Mosaic Virus / Q000737:chemistry*; D014771:Virion / Q000737:chemistry*; D014961:X-Ray Diffraction