title: Visualization of clathrin-mediated endocytosis in live Drosophila egg chambers.
abstract: In oviparous animals, clathrin-dependent endocytosis is often critical to stockpile a necessary supply of yolk within the maturing oocyte, which enables subsequent embryonic development. In the physically linked chains of maturing egg chambers within the Drosophila melanogaster ovary, a distinct, morphologically discernable subset undergoes a massive burst clathrin-mediated endocytosis to accumulate yolk in a process termed vitellogenesis. Here, we describe how to prepare isolated ovaries to follow endocytosis, and detail approaches to follow live uptake of soluble reporters into vitellogenic Drosophila egg chambers.
authors.affiliation: Department of Cell Biology, University of Pittsburgh School of Medicine, 3500 Terrace Street, 15261, Pittsburgh, PA, USA.
authors.forename: Anupma
authors.initials: A
authors.lastname: Jha
authors.forename: Linton M
authors.initials: LM
authors.lastname: Traub
mesh_terms: D000818:Animals; D002966:Clathrin / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology*; D005260:Female; D008853:Microscopy / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D010063:Ovum / Q000166:cytology* / Q000502:physiology*
chemical_list: D002966:Clathrin