title: Anti-adipogenic activity of the naturally occurring phenanthroindolizidine alkaloid antofine via direct suppression of PPARγ expression.
abstract: Antofine (ANTF) is a phenanthroindolizidine alkaloid isolated from the root of Cynanchum paniculatum Kitagawa (Asclepiadaceae), which is used as an herbal remedy for pain and inflammation. ANTF also possesses antiviral and antitumorigenic activities. In this study, we investigated the role of ANTF in adipogenesis. Chronic ABTF administration suppressed adipocyte differentiation and marker expression in a dose-dependent manner. Furthermore, acute administration of ANTF at early stages of differentiation process inhibited lipid droplet formation and adipogenic gene expression. ANTF Treatment decreased expression of PPARγ protein, a master transcription factor in the regulation of adipocyte differentiation, leading to a suppression of aP2 promoter activity. These results suggest that ANTF exerts potent anti-adipogenic effects via direct suppression of PPARγ protein expression, with consequent downregulation of adipogenic gene expression.
authors.affiliation: College of Pharmacy and Graduate school of Pharmaceutical Sciences, Global Top5 Research Program, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Korea, (phone: +82-2-32774369; fax: +82-2-32773760).
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Jang
authors.forename: Hyo Kyeong
authors.initials: HK
authors.lastname: Kim
authors.forename: Hana
authors.initials: H
authors.lastname: Jeong
authors.forename: Ye Seul
authors.initials: YS
authors.lastname: Lee
authors.forename: Mi Gyeong
authors.initials: MG
authors.lastname: Jeong
authors.forename: Su Jung
authors.initials: SJ
authors.lastname: Bae
authors.forename: Sanghee
authors.initials: S
authors.lastname: Kim
authors.forename: Sang Kook
authors.initials: SK
authors.lastname: Lee
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Hwang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D029062:Apocynaceae / Q000737:chemistry; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D066292:Lipid Droplets / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D047495:PPAR gamma / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D010618:Phenanthrolines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D001688:Biological Products; D007211:Indoles; D047495:PPAR gamma; D010618:Phenanthrolines; C473343:antofine
keywords: Adipocyte differentiation; Alkaloids; Anit-adipogenic activity; Antofine; PPARγ; Phenanthroindolizidine alkaloids