title: Milk oligosaccharides over time of lactation from different dog breeds.
abstract: The partnership of humans and dogs goes back to over 10'000 years, yet relatively little is known about a dog's first extra-uterine nutrition particularly when it comes to milk oligosaccharides. We set out to identify and quantify milk oligosaccharides over the course of lactation from different dog breeds (Labrador retriever, Schnauzer and 3 Alaskan husky crossbreeds). To this end, 2 different chromatographic methods with fluorescence and mass spectrometry detection were developed and one was validated for quantification. Besides lactose and lactose-sulphate, we identified 2 different trisaccharides composed of 3 hexose units, 3'sialyllactose (3'SL), 6'sialyllactose (6'SL), 2'fucosyllactose (2'FL), and a tetrasaccharide composed of 2 hexoses, an N-acetylhexosamine and a deoxyhexose. 3'SL was present at the highest levels in milk of all dog breeds starting at around 7.5 g/L and dropping to about 1.5 g/L in the first 10 days of lactation. 6'SL was about 10 times less abundant and 2'FL and the tetrasaccharide had rather varying levels in the milk of the different breeds with the tetrasaccharide only detectable in the Alaskan husky crossbreeds. The longitudinal and quantitative data of milk oligosaccharides from different dog breeds are an important basis to further our understanding on their specific biological roles and also on the specific nutritional requirements of lactating puppies.
authors.affiliation: Nestle Research Center, Nestec S.A., Lausanne, Switzerland.
authors.forename: Shirin
authors.initials: S
authors.lastname: Macias Rostami
authors.affiliation: Nestle Research Center, Nestec S.A., Lausanne, Switzerland.
authors.forename: Thierry
authors.initials: T
authors.lastname: Bénet
authors.affiliation: Nestle Purina Research, Nestec S.A., St. Louis, Missouri, United States of America.
authors.forename: Julie
authors.initials: J
authors.lastname: Spears
authors.affiliation: Nestle Purina Research, Nestec S.A., St. Louis, Missouri, United States of America.
authors.forename: Arleigh
authors.initials: A
authors.lastname: Reynolds
authors.affiliation: Nestle Purina Research, Nestec S.A., St. Louis, Missouri, United States of America.
authors.forename: Ebenezer
authors.initials: E
authors.lastname: Satyaraj
authors.affiliation: Nestle Research Center, Nestec S.A., Lausanne, Switzerland.
authors.forename: Norbert
authors.initials: N
authors.lastname: Sprenger
authors.affiliation: Nestle Research Center, Nestec S.A., Lausanne, Switzerland.
authors.forename: Sean
authors.initials: S
authors.lastname: Austin
mesh_terms: D000818:Animals; D001947:Breeding; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005260:Female; D007774:Lactation / Q000378:metabolism; D007785:Lactose / Q000032:analysis / Q000378:metabolism; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis* / Q000378:metabolism; D013045:Species Specificity; D013997:Time Factors
chemical_list: D009844:Oligosaccharides; D007785:Lactose