title: Colon cancer bears overexpression of OTUB1.
abstract: OBJECTIVE
  OTUB1 is a member of deubiquitinating enzymes; however, its expression and function in colon cancer are still unclear. The present study aimed at investigating the expression of OTUB1 in colon cancer and the relationship between the expression and some clinicopathologic parameters.
  
  
  METHODS
  Immunohistochemistry and quantitative real-time PCR were carried out in selected colon cancer and normal mucosa tissues.
  
  
  RESULTS
  The expression of OTUB1 protein in the colon cancer was significantly higher than normal mucosa, and the OTUB1 mRNA in colon cancer was also 3.15-fold higher than the normal mucosa. The higher expression of OTUB1 in colon cancer was related with tumor size, differentiation and lymph node metastasis.
  
  
  CONCLUSIONS
  OTUB1 may play an important role in colon cancer development and metastasis.
authors.affiliation: Department of Pathology, Qingdao University Medical College, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pathology, Qingdao Municipal Hospital, China.
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Jiang
authors.affiliation: Department of Pathology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, China. Electronic address: qdwangjigang@hotmail.com.
authors.forename: Ji-Gang
authors.initials: JG
authors.lastname: Wang
authors.affiliation: Department of Pathology, Qingdao Municipal Hospital, China. Electronic address: chenhua62@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
mesh_terms: D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D000072017:Deubiquitinating Enzymes; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D000072017:Deubiquitinating Enzymes; C538826:OTUB1 protein, human; D003546:Cysteine Endopeptidases
keywords: Colon cancer; Immunohistochemistry; OTUB1; Quantitative real-time PCR