title: Identification and characterization of Mini1, a gene regulating rice shoot development.
abstract: The aerial parts of higher plants are generated from the shoot apical meristem (SAM). In this study, we isolated a small rice (Oryza sativa L.) mutant that showed premature termination of shoot development and was named mini rice 1 (mini1). The mutant was first isolated from a japonica cultivar Zhonghua11 (ZH11) subjected to ethyl methanesulfonate (EMS) treatment. With bulked segregant analysis (BSA) and map-based cloning method, Mini1 gene was finally fine-mapped to an interval of 48.6 kb on chromosome 9. Sequence analyses revealed a single base substitution from G to A was found in the region, which resulted in an amino acid change from Gly to Asp. The candidate gene Os09g0363900 was predicted to encode a putative adhesion of calyx edges protein ACE (putative HOTHEAD precursor) and genetic complementation experiment confirmed the identity of Mini1. Os09g0363900 contains glucose-methanol-choline (GMC) oxidoreductase and NAD(P)-binding Rossmann-like domain, and exhibits high similarity to Arabidopsis HOTHEAD (HTH). Expression analysis indicated Mini1 was highly expressed in young shoots but lowly in roots and the expression level of most genes involved in auxin biosynthesis and signal transduction were reduced in mutant. We conclude that Mini1 plays an important role in maintaining SAM activity and promoting shoot development in rice.
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, 359 Tiyuchang Road, Hangzhou, 310006, China; College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Fang
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Haiping
authors.initials: H
authors.lastname: Yu
authors.forename: Zhenyuan
authors.initials: Z
authors.lastname: Shi
authors.forename: Guosheng
authors.initials: G
authors.lastname: Xiong
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Dali
authors.initials: D
authors.lastname: Zeng
authors.forename: Guangheng
authors.initials: G
authors.lastname: Zhang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Gao
authors.forename: Guojun
authors.initials: G
authors.lastname: Dong
authors.forename: Meixian
authors.initials: M
authors.lastname: Yan
authors.forename: Longbiao
authors.initials: L
authors.lastname: Guo
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
mesh_terms: D000595:Amino Acid Sequence; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D056726:Genetic Association Studies; D005816:Genetic Complementation Test; D007210:Indoleacetic Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D020161:Physical Chromosome Mapping; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics* / Q000254:growth & development*; D030821:Plants, Genetically Modified; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D015398:Signal Transduction / Q000235:genetics; D059467:Transcriptome / Q000235:genetics
chemical_list: D007210:Indoleacetic Acids; D010940:Plant Proteins
keywords: HOTHEAD; mini; rice; shoot development