title: An emerging water contaminant, semicarbazide, exerts an anti-estrogenic effect in zebrafish (Danio rerio).
abstract: To determine the endocrine disrupting effect of semicarbazide, an emerging water contaminant, the changes in transcript levels of hepatic estrogen-response genes including vitellogenin-1 (vtg-1), estrogen receptor α (ERα), and estrogen receptor β (ERβ) were measured in male and female zebrafish exposed to semicarbazide with or without exogenous 17β-estradiol (E2). Exposure of male zebrafish to semicarbazide for 96 h or 28 days resulted in no significant induction in hepatic vtg-1, ERα, or ERβ mRNA expression, indicating that semicarbazide has no estrogenic effect. However, a remarkable anti-estrogenic effect of semicarbazide was demonstrated: semicarbazide treatment of female zebrafish for 96 h and 28 days resulted in significant decreases in transcript levels of vtg-1, ERα, and ERβ, as well as decreases in the gonadosomatic index level after 28 days. Moreover, semicarbazide exposure significantly inhibited the induction of vtg-1, ERα and ERβ mRNA by E2 when male zebrafish were co-exposed for 28 days.
authors.affiliation: Marine Life Science College, Ocean University of China, 5 Yushan Road, Qingdao, 266003, Shandong, People's Republic of China.
authors.forename: Su
authors.initials: S
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
authors.forename: Xiaona
authors.initials: X
authors.lastname: Zhang
authors.forename: Linlin
authors.initials: L
authors.lastname: Guo
authors.forename: Shaoguo
authors.initials: S
authors.lastname: Ru
mesh_terms: D000818:Animals; D052244:Endocrine Disruptors / Q000378:metabolism / Q000633:toxicity*; D004958:Estradiol / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism; D005260:Female; D008099:Liver / Q000378:metabolism; D008297:Male; D011960:Receptors, Estrogen / Q000378:metabolism*; D012663:Semicarbazides / Q000633:toxicity*; D014819:Vitellogenins / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D004967:Estrogens; D011960:Receptors, Estrogen; D012663:Semicarbazides; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; C010059:carbamylhydrazine; D004958:Estradiol