title: Genotoxicological response of the common carp (Cyprinus carpio) exposed to spring water in Tlaxcala, México.
abstract: This study evaluated the genotoxic impact of anthropic activities in Huactzinco Spring, using Cyprinus carpio as a biomonitor. In situ and in vivo experimental designs were compared by means of simultaneous 2-week exposures. The water from the spring generated mean micronuclei frequency values (108.6 ± 32 MN/1,000) and DNA fragmentation values (143.4 ± 35 au) which were statistically higher than those for the negative control (10.9 ± 6 MN/1,000 and 67.6 ± 23 au). The in situ and in vivo experiments supported one another. The comet assay proved to be the most sensitive test, with an EC50 value (11.4 % ± 3.4 %) being less than that determined for the micronuclei test (54.8 % ± 3.2 %). The results of this study confirm the usefulness of C. carpio as an environmental contamination biomonitor, and suggest that Huactzinco Spring water constitutes a latent risk to human health and the environment.
authors.affiliation: Centro de Investigación en Genética y Ambiente, Universidad Autónoma de Tlaxcala, Km 10.5 Autopista San Martín-Tlaxcala, CP 90120, Ixtacuixtla, Tlax., Mexico, mirosgn@yahoo.com.mx.
authors.forename: Edelmira
authors.initials: E
authors.lastname: García-Nieto
authors.forename: Libertad
authors.initials: L
authors.lastname: Juárez-Santacruz
authors.forename: Elizabeth
authors.initials: E
authors.lastname: García-Gallegos
authors.forename: Joselin
authors.initials: J
authors.lastname: Tlalmis-Zempoalteca
authors.forename: Claudia
authors.initials: C
authors.lastname: Romo-Gómez
authors.forename: Arturo
authors.initials: A
authors.lastname: Torres-Dosal
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002347:Carps / Q000235:genetics / Q000378:metabolism*; D020552:Comet Assay; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods / Q000706:statistics & numerical data*; D006801:Humans; D008800:Mexico; D015162:Micronucleus Tests; D060985:Natural Springs / Q000737:chemistry*; D012621:Seasons; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical