title: Response of mice and ferrets to a monovalent influenza A (H7N9) split vaccine.
abstract: In early spring 2013, the emergence of the influenza A (H7N9) virus in humans in Eastern China raised concerns of a new influenza pandemic. Development of a safe and effective H7N9 influenza vaccine is urgently needed. To this end, we first synthesized the hemagglutinin (HA) and neuraminidase (NA) genes of the influenza A (H7N9) virus A/AnHui/1/2013. Using reverse genetics, we rescued a reassortant virus (H7N9/PR8) that contained the HA and NA genes from wild-type H7N9 and six genes encoding internal proteins from the A/Puerto Rico/8/34 (PR8) virus. Next, the pathogenicity of the reassortant virus was evaluated both in vivo and in vitro. We found that the virus was non-pathogenic in mice and was stable after serial passaging in eggs. Furthermore, we found that a monovalent influenza A (H7N9) split vaccine prepared from the virus was immunogenic in mice and ferrets. When given intramuscularly, the vaccine (two doses of at least 15-µg) completely protected mice from normally lethal wild-type H7N9 virus challenge. In summary, our H7N9 vaccine, developed over a short time, is a potential candidate for further clinical evaluation and human use.
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Hongjing
authors.initials: H
authors.lastname: Gu
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China; Department of Pathogenic Biology and Medical Immunology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Zhongpeng
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Liangyan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xing
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Chengcai
authors.initials: C
authors.lastname: Lai
authors.affiliation: 302 Military Hospital, Beijing, China.
authors.forename: Peirui
authors.initials: P
authors.lastname: Zhang
authors.affiliation: 302 Military Hospital, Beijing, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Li
authors.affiliation: 302 Military Hospital, Beijing, China.
authors.forename: Keming
authors.initials: K
authors.lastname: Zhang
authors.affiliation: 302 Military Hospital, Beijing, China.
authors.forename: Zhouhai
authors.initials: Z
authors.lastname: Wang
authors.affiliation: 302 Military Hospital, Beijing, China.
authors.forename: Shaogeng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China; 302 Military Hospital, Beijing, China.
authors.forename: Penghui
authors.initials: P
authors.lastname: Yang
mesh_terms: D000818:Animals; D002213:Capsid / Q000648:ultrastructure; D002645:Chickens; D002522:Chlorocebus aethiops; D005260:Female; D005289:Ferrets; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000235:genetics / Q000276:immunology* / Q000648:ultrastructure; D007252:Influenza Vaccines / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D008807:Mice, Inbred BALB C; D059386:Reverse Genetics; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D007252:Influenza Vaccines; D014613:Vaccines, Attenuated