title: A new flavonol glycoside from the medicinal halophyte Suaeda fruticosa.
abstract: A new flavonol glycoside, namely 3-(α-rhamnopyranosyl-(1 → 2)-[β-xylopyranosyl-(1 → 6)]-β-glucopyranosyloxy) isorhamnetin was reported from methanol extracts of aerial parts of Suaeda fruticosa for the first time. In this work, liquid chromatography coupled to atmospheric pressure chemical ionisation mass spectrometry, high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy were used to identify this new compound. Structure was elucidated on the basis of extensive spectroscopic analysis, including HSQC, HMBC and (1)H-(1)H COSY. Antioxidant potentialities of a pure compound were evaluated. The estimation of antioxidant capacities using oxygen radical absorbance capacity (ORAC method) and a cell based-assay (WS1) indicated that this new flavonol exhibited the highest antioxidant activities with an ORAC value of 5.0 ± 0.3 μmol Trolox/μmol and inhibited the tBH-induced oxidation of 2',7'-dichlorofluorescin with an IC50 value of 4.9 ± 0.6 μM.
authors.affiliation: a Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie à la Technopôle de Borj-Cédria (CBBC) , BP 901, 2050 Hammam-Lif , Tunisia.
authors.forename: Samia
authors.initials: S
authors.lastname: Oueslati
authors.forename: Riadh
authors.initials: R
authors.lastname: Ksouri
authors.forename: André
authors.initials: A
authors.lastname: Pichette
authors.forename: Serge
authors.initials: S
authors.lastname: Lavoie
authors.forename: Karl
authors.initials: K
authors.lastname: Girard-Lalancette
authors.forename: Vakhtang
authors.initials: V
authors.lastname: Mshvildadze
authors.forename: Chedly
authors.initials: C
authors.lastname: Abdelly
authors.forename: Jean
authors.initials: J
authors.lastname: Legault
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D019663:Chenopodiaceae / Q000737:chemistry*; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005452:Fluoresceins / Q000378:metabolism; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D010946:Plants, Medicinal / Q000737:chemistry*; D055051:Salt-Tolerant Plants / Q000737:chemistry*; D014416:Tunisia
chemical_list: C000592865:3-(alpha-rhamnopyranosyl-(1-2)-(beta-xylopyranosyl-(1-6))-beta-glucopyranosyloxy)isorhamnetin; D000975:Antioxidants; D044948:Flavonols; D005452:Fluoresceins; D006027:Glycosides; C037631:2',7'-dichlorofluorescein
keywords: Suaeda fruticosa; flavonol glycoside; high-performance liquid chromatography; mass spectrometry; nuclear magnetic resonance; oxygen radical absorbance capacity