title: Biogenic copper oxide nanoparticles synthesis using Tabernaemontana divaricate leaf extract and its antibacterial activity against urinary tract pathogen.
abstract: This investigation explains the biosynthesis and characterization of copper oxide nanoparticles from an Indian medicinal plant by an eco-friendly method. The main objective of this study is to synthesize copper oxide nanoparticles from Tabernaemontana divaricate leaves through a green chemistry approach. Highly stable, spherical copper oxide nanoparticles were synthesized by using 50% concentration of Tabernaemontana leaf extract. Formation of copper oxide nanoparticles have been characterized by UV-Vis absorption spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDX) and transmission electron microscopy (TEM) analysis. All the analyses revealed that copper oxide nanoparticles were 48±4nm in size. Functional groups and chemical composition of copper oxide were also confirmed. Antimicrobial activity of biogenic copper oxide nanoparticles were investigated and maximum zone of inhibition was found in 50μg/ml copper oxide nanoparticles against urinary tract pathogen (Escherichia coli).
authors.affiliation: School of Science and Engineering, Teesside University, Middlesbrough TS1 3BA, Tees Valley, UK; Department of Biotechnology, School of Life Sciences, Karpagam University, Eachanari post, Coimbatore 641 021, Tamil Nadu, India. Electronic address: rajeshwarishivaraj@gmail.com.
authors.forename: Rajeshwari
authors.initials: R
authors.lastname: Sivaraj
authors.affiliation: School of Science and Engineering, Teesside University, Middlesbrough TS1 3BA, Tees Valley, UK.
authors.forename: Pattanathu K S M
authors.initials: PK
authors.lastname: Rahman
authors.affiliation: Department of Biotechnology, School of Life Sciences, Karpagam University, Eachanari post, Coimbatore 641 021, Tamil Nadu, India.
authors.forename: P
authors.initials: P
authors.lastname: Rajiv
authors.affiliation: Department of Biotechnology, School of Life Sciences, Karpagam University, Eachanari post, Coimbatore 641 021, Tamil Nadu, India.
authors.forename: Hasna Abdul
authors.initials: HA
authors.lastname: Salam
authors.affiliation: Faculty of Chemistry, Government Arts College, Udumalpet 642 126, Tamil Nadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Venckatesh
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000188:drug therapy; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D030014:Tabernaemontana / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D003300:Copper; C030973:cupric oxide
keywords: Antibacterial activity; Copper oxide nanoparticles; FT-IR; SEM; TEM