title: Identification of transcription factors activated in thymic epithelial cells during embryonic thymus development.
abstract: Differentiation of many immune-related cells is controlled by the expression levels and the activation status of transcription factors (TFs). We here describe a method to identify candidate TFs activated during the development of thymic epithelial cells (TECs) in the embryo. RNAs are isolated from fetal thymic organ cultures of wild-type and mutant mice and are subsequently analyzed by using a combination of comprehensive expression analysis and in silico data analysis in order to predict the TFs that might be activated.
authors.affiliation: Division of Cellular and Molecular Biology, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokane-dai, Minato-ku, Tokyo, 108-8639, Japan, taishin@ims.u-tokyo.ac.jp.
authors.forename: Taishin
authors.initials: T
authors.lastname: Akiyama
authors.forename: Junwen
authors.initials: J
authors.lastname: Qin
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ohshima
authors.forename: Jun-ichiro
authors.initials: J
authors.lastname: Inoue
mesh_terms: D000818:Animals; D030561:Databases, Nucleic Acid; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D004867:Equipment Design; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D009924:Organ Culture Techniques / Q000295:instrumentation / Q000379:methods; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D013950:Thymus Gland / Q000196:embryology* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014157:Transcription Factors; D012313:RNA