title: Physicochemical parameters optimization, and purification of phycobiliproteins from the isolated Nostoc sp.
abstract: The present study investigated the effects of several physicochemical parameters on the improvement of phycobiliproteins (especially phycocyanin) synthesis in a newly isolated species of Nostoc sp. Standard BG11₀ medium was modified to enhance the biomass productivity in different photobioreactors. The initial pH of 8, light intensity of 40 μmol m(-2)s(-1), temperature of 35 °C, diurnal cycle of 16:8 h (light:dark regime), 75.48 μM Na₂CO₃ and 17.65 mM NaNO₃ were found most suitable for the phycobiliproteins synthesis. Cyanobacteria exhibited chromatic adaptation, causing overexpression of phycocyanin in red and phycoerythrin in green light. The maximum phycobiliproteins yield of 0.13 gg(-1) dry cell weight was obtained in green light. Phycocyanin was further purified using thin layer chromatography (TLC), anion exchange chromatography and SDS-PAGE (denaturing gel) electrophoresis.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Eldin M
authors.initials: EM
authors.lastname: Johnson
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Kanhaiya
authors.initials: K
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India. Electronic address: ddas.iitkgp@gmail.com.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Das
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors; D002254:Carbonates / Q000494:pharmacology; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D046937:Nostoc / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D052979:Phycobiliproteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002254:Carbonates; D003470:Culture Media; D052979:Phycobiliproteins; C005686:sodium carbonate
keywords: Cyanobacteria; Light; Phycocyanin; SDS–PAGE; pH