title: The rolC gene increases caffeoylquinic acid production in transformed artichoke cells.
abstract: Caffeoylquinic acids are found in artichokes, and they are currently considered important therapeutic or preventive agents for treating Alzheimer's disease and diabetes. We transformed artichoke [the cultivated cardoon or Cynara cardunculus var. altilis DC (Asteraceae)] with the rolC gene, which is a known inducer of secondary metabolism. High-performance liquid chromatography with UV and high-resolution mass spectrometry (HPLC-UV-HRMS) revealed that the predominant metabolites synthesized in the transgenic calli were 1,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and chlorogenic acid. The rolC-transformed calli contained 1.5% caffeoylquinic acids by dry weight. The overall production of these metabolites was three times higher than that of the corresponding control calli. The enhancing effect of rolC remained stable over long-term cultivation.
authors.affiliation: Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, 159 Stoletija Str., Vladivostok, 690022, Russia.
authors.forename: Y V
authors.initials: YV
authors.lastname: Vereshchagina
authors.forename: V P
authors.initials: VP
authors.lastname: Bulgakov
authors.forename: V P
authors.initials: VP
authors.lastname: Grigorchuk
authors.forename: V G
authors.initials: VG
authors.lastname: Rybin
authors.forename: G N
authors.initials: GN
authors.lastname: Veremeichik
authors.forename: G K
authors.initials: GK
authors.lastname: Tchernoded
authors.forename: T Y
authors.initials: TY
authors.lastname: Gorpenchenko
authors.forename: O G
authors.initials: OG
authors.lastname: Koren
authors.forename: N H T
authors.initials: NH
authors.lastname: Phan
authors.forename: N T
authors.initials: NT
authors.lastname: Minh
authors.forename: L T
authors.initials: LT
authors.lastname: Chau
authors.forename: Y N
authors.initials: YN
authors.lastname: Zhuravlev
mesh_terms: D002934:Cinnamates / Q000378:metabolism; D031943:Cynara scolymus / Q000166:cytology / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D002934:Cinnamates; D010940:Plant Proteins; C472707:caffeoylquinic acid; D011801:Quinic Acid; C100257:cynarine