title: Inducing the oxidative stress response in Escherichia coli improves the quality of a recombinant protein: magnesium chelatase ChlH.
abstract: The ∼150kDa ChlH subunit of magnesium chelatase from Oryza sativa, Hordeum vulgare and Chlamydomonas reinhardtii have been heterologously expressed in Escherichiacoli. The active soluble protein is found as both a multimeric and a monomeric form. The multimeric ChlH appears to be oxidatively damaged but monomer production is favoured in growth conditions that are known to cause an oxidative stress response in E.coli. Inducing an oxidative stress response may be of general utility to improve the quality of proteins expressed in E. coli. The similar responses of ChlH's from the three different species suggest that oligomerization of oxidatively damaged ChlH may have a functional role in the chloroplast, possibly as a signal of oxidative stress or damage.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, NSW 2109, Australia; Carlsberg Laboratory, Gamle Carlsberg Vej 10, 1799 Copenhagen V, Denmark.
authors.forename: André H
authors.initials: AH
authors.lastname: Müller
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, NSW 2109, Australia.
authors.forename: Artur
authors.initials: A
authors.lastname: Sawicki
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Shuaixiang
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, NSW 2109, Australia.
authors.forename: Shabnam Tarahi
authors.initials: ST
authors.lastname: Tabrizi
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Meizhong
authors.initials: M
authors.lastname: Luo
authors.affiliation: Carlsberg Laboratory, Gamle Carlsberg Vej 10, 1799 Copenhagen V, Denmark.
authors.forename: Mats
authors.initials: M
authors.lastname: Hansson
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, NSW 2109, Australia. Electronic address: robert.willows@mq.edu.au.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Willows
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000201:enzymology; D004926:Escherichia coli / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D001467:Hordeum / Q000201:enzymology; D008190:Lyases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D055503:Protein Multimerization / Q000502:physiology*; D021122:Protein Subunits / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D021122:Protein Subunits; D011994:Recombinant Proteins; D008190:Lyases; C086788:magnesium chelatase
keywords: Chelatase; Chlorophyll biosynthesis; Magnesium; Oxidative stress; Protoporphyrin