title: Development of hybrid organic-inorganic surface imprinted Mn-doped ZnS QDs and their application as a sensing material for target proteins.
abstract: Applying molecular imprinting techniques to the surface of functionalized quantum dots (QDs) allows the preparation of molecularly imprinted polymers (MIPs) with accessible, surface exposed binding sites and excellent optical properties. This paper demonstrates a new strategy for producing such hybrid organic-inorganic imprinted Mn-doped ZnS QDs for specific recognition of bovine hemoglobin. The technique provides surface grafting imprinting in aqueous solutions using amino modified Mn-doped ZnS QDs as supports, acrylamide and methacrylic acid as functional monomers, γ-methacryloxypropyl trimethoxy silane as the grafting agent, and bovine hemoglobin as a template. The amino propyl functional monomer layer directs the selective occurrence of imprinting polymerization at the QDs surface through copolymerization of grafting agents with functional monomers, but also acts as an assistive monomer to drive the template into the formed polymer shells to create effective recognition sites. Using MIP-QDs composites as a fluorescence sensing material, trace amounts of bovine hemoglobin are signaled with high selectivity by emission intensity changes of Mn-doped ZnS QDs, which is embedded into the imprinted polymers.
authors.affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China. Electronic address: jsutanlei@gmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Tan
authors.affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Huang
authors.affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
authors.forename: Rongfei
authors.initials: R
authors.lastname: Peng
authors.affiliation: Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Department of Chemistry, South China Normal University, Guangzhou 510006, China.
authors.forename: Youwen
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Guangdong Key Laboratory of Green Processing of Natural Products and Product Safety, School of Light Industry and Food Science, South China University of Technology, Guangzhou 510641, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D006454:Hemoglobins / Q000032:analysis*; D006456:Hemoglobinuria / Q000175:diagnosis / Q000652:urine*; D006801:Humans; D008345:Manganese / Q000737:chemistry*; D054802:Molecular Imprinting; D045663:Quantum Dots / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013440:Sulfides / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D006454:Hemoglobins; D013440:Sulfides; D017967:Zinc Compounds; D008345:Manganese; C031238:zinc sulfide
keywords: Fluorescence probes; Hybrid composite material; Molecularly imprinted; Quantum dots