title: Immune cell function assay does not identify biopsy-proven pediatric renal allograft rejection or infection.
abstract: Management of pediatric renal transplant patients involves multifactorial monitoring modalities to ensure allograft survival and prevent opportunistic infection secondary to immunosuppression. An ICFA, which utilizes CD4 T-cell production of ATP to assess immune system status, has been used to monitor transplant recipients and predict susceptibility of patients to rejection or infection. However, the validity of this assay to reflect immune status remains unanswered. In a two-yr retrospective study that included 31 pediatric renal transplant recipients, 42 patient blood samples were analyzed for immune cell function levels, creatinine, WBC (white blood cell) count, immunosuppressive drug levels, and viremia, concurrent with renal biopsy. T-cell ATP production as assessed by ICFA levels did not correlate with allograft rejection or with the presence or absence of viremia. ICFA levels did not correlate with serum creatinine or immunosuppressive drug levels, but did correlate with WBC count. The ICFA is unreliable in its ability to reflect immune system status in pediatric renal transplantation. Further investigation is necessary to develop methods that will accurately predict susceptibility of pediatric renal transplant recipients to allograft rejection and infection.
authors.affiliation: Department of Pediatrics, Lucile Packard Children's Hospital, Stanford University School of Medicine, Palo Alto, CA, USA; Department of Pediatrics, Kaiser Permanente Santa Clara Medical Center, Santa Clara, CA, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Ryan
authors.forename: A
authors.initials: A
authors.lastname: Chaudhuri
authors.forename: W
authors.initials: W
authors.lastname: Concepcion
authors.forename: P C
authors.initials: PC
authors.lastname: Grimm
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000293:Adolescent; D064591:Allografts; D001706:Biopsy; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis*; D006085:Graft Survival; D006801:Humans; D007165:Immunosuppression Therapy; D007166:Immunosuppressive Agents / Q000009:adverse effects; D007223:Infant; D007668:Kidney / Q000276:immunology; D016030:Kidney Transplantation; D007958:Leukocyte Count; D008297:Male; D009894:Opportunistic Infections / Q000175:diagnosis; D051437:Renal Insufficiency / Q000150:complications / Q000276:immunology / Q000601:surgery*; D012189:Retrospective Studies; D014766:Viremia / Q000097:blood; D055815:Young Adult
chemical_list: D007166:Immunosuppressive Agents; D000255:Adenosine Triphosphate
keywords: adenosine triphosphate; immune function; infection; kidney; pediatric; rejection; transplantation