title: In vitro and in vivo studies of BMP-2-loaded PCL-gelatin-BCP electrospun scaffolds.
abstract: To confirm the effect of recombinant human bone morphogenetic protein-2 (BMP-2) for bone regeneration, BMP-2-loaded polycaprolactone (PCL)-gelatin (Gel)-biphasic calcium phosphate (BCP) fibrous scaffolds were fabricated using the electrospinning method. The electrospinning process to incorporate BCP nanoparticles into the PCL-Gel scaffolds yielded an extracellular matrix-like microstructure that was a hybrid system composed of nano- and micro-sized fibers. BMP-2 was homogeneously loaded on the PCL-Gel-BCP scaffolds for enhanced induction of bone growth. BMP-2 was initially released at high levels, and then showed sustained release behavior for 31 days. Compared with the PCL-Gel-BCP scaffold, the BMP-2-loaded PCL-Gel-BCP scaffold showed improved cell proliferation and cell adhesion behavior. Both scaffold types were implanted in rat skull defects for 4 and 8 weeks to evaluate the biological response under physiological conditions. Remarkable bone regeneration was observed in the BMP-2/PCL-Gel-BCP group. These results suggest that BMP-2-loaded PCL-Gel-BCP scaffolds should be considered for potential bone tissue engineering applications.
authors.affiliation: 1 Department of Regenerative Medicine, College of Medicine, Soonchunhyang University , Cheonan, Republic of Korea.
authors.forename: Bo-Ram
authors.initials: BR
authors.lastname: Kim
authors.forename: Thuy Ba Linh
authors.initials: TB
authors.lastname: Nguyen
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Min
authors.forename: Byong-Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D001861:Bone Regeneration / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D008297:Male; D051379:Mice; D010012:Osteogenesis / Q000502:physiology; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin