title: Influence of curcumin on HOTAIR-mediated migration of human renal cell carcinoma cells.
abstract: BACKGROUND
  This study investigated the influence of curcumin on HOX transcript antisense RNA (HOTAIR)- mediated migration of cultured renal cell carcinoma (RCC) cells.
  
  
  METHODS
  Five RCC cell lines (769-P, 769-P-vector, 769-P-HOTAIR, 786-0, and Kert-3 ) were maintained in vitro. The expression of HOTAIR mRNA was determined by quantitative real-time PCR and cell migration was measured by transwell migration assay. The effects of different concentrations of curcumin (0 to 80 μmol/L) on cell proliferation was determined by the CCK-8 assay and influence of non-toxic levels (0 to 10 μM) on the migration of RCC cells was also determined.
  
  
  RESULTS
  Comparison of the 5 cell lines indicated a correlation between HOTAIR mRNA expression and cell migration. In particular, the migration of 769-P-HOTAIR cells was significantly higher than that of 769-P-vector cells. Curcumin at 2.5-10 μM had no evident toxicity against RCC cells, but inhibited cell migration in a concentration-dependent manner.
  
  
  CONCLUSIONS
  HOTAIR expression is correlated with the migration of RCC cells, and HOTAIR may be involved in the curcumin-induced inhibition of RCC metastasis.
authors.affiliation: The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, China E-mail : cr_ane@163.com.
authors.forename: Chang-Song
authors.initials: CS
authors.lastname: Pei
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Wu
authors.forename: Fan-Tian
authors.initials: FT
authors.lastname: Fan
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.forename: Cun-Si
authors.initials: CS
authors.lastname: Shen
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Pan
mesh_terms: D002292:Carcinoma, Renal Cell / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000188:drug therapy* / Q000473:pathology; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C561566:HOTAIR long untranslated RNA, human; D062085:RNA, Long Noncoding; D003474:Curcumin