title: Robust detection of tyrosine phosphatase activity by coupling chymotrypsin-assisted selective peptide cleavage and a graphene oxide-based fluorescent platform.
abstract: A versatile graphene oxide (GO)-based fluorescent assay is developed for the detection of protein tyrosine phosphatase activity by coupling with a chymotrypsin-assisted selective peptide cleavage reaction.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P. R. China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Wang
authors.forename: Chenghui
authors.initials: C
authors.lastname: Liu
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Fan
authors.forename: Yucong
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques; D002918:Chymotrypsin / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010087:Oxides / Q000737:chemistry*; D010446:Peptide Fragments / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D010087:Oxides; D010446:Peptide Fragments; D014443:Tyrosine; D006108:Graphite; D017027:Protein Tyrosine Phosphatases; D002918:Chymotrypsin