title: Role of Candida albicans-secreted aspartyl proteinases (Saps) in severe early childhood caries.
abstract: Candida albicans is strongly associated with severe early childhood caries (S-ECC). However, the roles of secreted aspartyl proteinases (Saps), an important virulence factor of C. albicans, in the progress of S-ECC are not clear. In our study, the Saps activities were evaluated by the yeast nitrogen base-bovine serum albumi (YNB-BSA) agar plate method and by the MTT method with bovine serum albumin (BSA) as the substrate. Genotypes of C. albicans and gene expression of Sap1-5 were evaluated. The relationships of Saps activities and genotypes with S-ECC were analyzed. The results showed that enzyme activities of Saps in the S-ECC group were significantly higher than those in the caries free (CF) group (p<0.05). Genotypes A, B and C were detected in the S-ECC group, and genotypes A and C were detected in the CF group. In the genotype A group, Saps activity in the S-ECC group was significantly different from that in the CF group (p<0.05). The gene expression level of Sap1 in the S-ECC group was significantly higher than that in the CF group (p=0.001), while Sap4 expression was significantly lower than that in the CF group (p=0.029). It can be concluded that Sap1-5 are the predominant proteinase genes expressed in C. albicans from dental biofilm and Sap1 may play an important role in the development of S-ECC.
authors.affiliation: Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, China. wendysums@hotmail.com.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Li
authors.affiliation: Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, China. yudsh@mail.sysu.edu.cn.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Yu
authors.affiliation: Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, China. gshuo@mail3.sysu.edu.cn.
authors.forename: Shuo
authors.initials: S
authors.lastname: Gao
authors.affiliation: Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, China. linjiach@mail.sysu.edu.cn.
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Lin
authors.affiliation: Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, China. chzhuoy@mail2.sysu.edu.cn.
authors.forename: Zhuoyu
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, China. zhaowei3@mail.sysu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000818:Animals; D057055:Aspartic Acid Proteases / Q000235:genetics / Q000378:metabolism*; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D002675:Child, Preschool; D003731:Dental Caries / Q000382:microbiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D012335:RNA, Ribosomal; C065039:RNA, ribosomal, 25S; D012710:Serum Albumin, Bovine; D057055:Aspartic Acid Proteases