title: Cryopreservation of epididymal sperm from ibexes (Capra pyrenaica) using short equilibration time with glycerol.
abstract: Two experiments were conducted to study the effect of shortening the equilibration time with the cryoprotectant glycerol before freezing epididymal sperm recovered postmortem from Iberian ibex. In the first experiment, the standard equilibration time of 3 hours was compared with 2 hours, and subjective sperm motility and quality of movement were greater (P < 0.05) in the latter group. In the second experiment, reducing the equilibration time from 2 hours to 15 minutes did not affect sperm motility (evaluated subjectively and objectively), viability, acrosomal integrity, or membrane functional integrity. In conclusion, shortening the equilibration time can be used as a technique to simplify the cryopreservation process and this provides practical advantages under field conditions.
authors.affiliation: Departamento de Reproducción Animal, SGIT-INIA, Madrid, Spain; Cnpq, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Pradiee
authors.affiliation: Departamento de Reproducción Animal, SGIT-INIA, Madrid, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Esteso
authors.affiliation: Departamento de Reproducción Animal, SGIT-INIA, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Castaño
authors.affiliation: Departamento de Reproducción Animal, SGIT-INIA, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Toledano-Díaz
authors.affiliation: Departamento de Reproducción Animal, SGIT-INIA, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: López-Sebastián
authors.affiliation: Departamento de Reproducción Animal, SGIT-INIA, Madrid, Spain. Electronic address: moreno@inia.es.
authors.forename: J
authors.initials: J
authors.lastname: Santiago-Moreno
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D004822:Epididymis / Q000166:cytology*; D005990:Glycerol; D006041:Goats / Q000502:physiology*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013997:Time Factors
chemical_list: D005990:Glycerol
keywords: Cryopreservation; Epididymal spermatozoa; Postmortem recovery