title: Association of CXCR1 polymorphisms with apoptosis, necrosis and concentration of milk neutrophils in early lactating dairy heifers.
abstract: Associations between polymorphisms in the candidate gene CXCR1, encoding the chemokine (C-X-C motif) receptor 1, and udder health have been identified before. In the present study, associations between the CXCR1 genotype (whole coding region) and apoptosis, necrosis, and concentration of milk polymorphonuclear neutrophilic leukocyte (PMNL) of 292 quarters belonging to 73 early lactating dairy heifers were studied. In uninfected quarters, % milk PMNL apoptosis was higher in c.980GG heifers [least squares means (LSM) 27%] compared to c.980AG heifers (LSM 16%), whereas in infected quarters, % milk PMNL apoptosis was higher in c.642GG heifers (LSM 29%) compared to c.642AG heifers (LSM 18%). Differences in milk PMNL concentration between infected and uninfected quarters were smaller in c.980AG heifers than in c.980GG heifers. An association between the CXCR1 genotype and necrosis of milk PMNL could not be demonstrated. Results indicate that CXCR1 polymorphisms influence viability and concentration of milk PMNL and provide a foundation for future research.
authors.affiliation: M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Belgium. Electronic address: Joren.Verbeke@UGent.be.
authors.forename: Joren
authors.initials: J
authors.lastname: Verbeke
authors.affiliation: M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Sofie
authors.initials: S
authors.lastname: Piepers
authors.affiliation: Animal Genetics Laboratory, Department of Nutrition, Genetics, and Ethology, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: Peelman
authors.affiliation: Animal Genetics Laboratory, Department of Nutrition, Genetics, and Ethology, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Mario
authors.initials: M
authors.lastname: Van Poucke
authors.affiliation: M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Sarne
authors.initials: S
authors.lastname: De Vliegher
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002417:Cattle; D002452:Cell Count; D002470:Cell Survival / Q000235:genetics; D003612:Dairying; D005260:Female; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics; D007774:Lactation / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000276:immunology; D008892:Milk / Q000166:cytology*; D009336:Necrosis / Q000235:genetics; D009504:Neutrophils / Q000473:pathology*; D011110:Polymorphism, Genetic / Q000235:genetics*; D023062:Receptors, Interleukin-8A / Q000235:genetics*
chemical_list: D023062:Receptors, Interleukin-8A
keywords: Apoptosis; CXCR1; Dairy heifer; Necrosis; Polymorphonuclear neutrophilic leukocyte