title: Influence of very short patch mismatch repair on SOS inducing lesions after aminoglycoside treatment in Escherichia coli.
abstract: Low concentrations of aminoglycosides induce the SOS response in Vibrio cholerae but not in Escherichia coli. In order to determine whether a specific factor present in E. coli prevents this induction, we developed a genetic screen where only SOS inducing mutants are viable. We identified the vsr gene coding for the Vsr protein of the very short patch mismatch repair (VSPR) pathway. The effect of mismatch repair (MMR) mutants was also studied. We propose that lesions formed upon aminoglycoside treatment are preferentially repaired by VSPR without SOS induction in E. coli and by MMR when VSPR is impaired.
authors.affiliation: Institut Pasteur, Unité Plasticité du Génome Bactérien, Département Génomes et Génétique, 25 rue du docteur Roux, 75015 Paris, France; CNRS, UMR3525, Paris, France. Electronic address: baharogl@pasteur.fr.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Baharoglu
authors.affiliation: Institut Pasteur, Unité Plasticité du Génome Bactérien, Département Génomes et Génétique, 25 rue du docteur Roux, 75015 Paris, France; CNRS, UMR3525, Paris, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Mazel
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D053843:DNA Mismatch Repair; D045643:DNA Repair Enzymes / Q000235:genetics / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D013014:SOS Response, Genetics
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D004706:Endodeoxyribonucleases; C000596250:Vsr protein, E coli; D045643:DNA Repair Enzymes
keywords: Antibiotics; DNA repair; SOS; Sub-MIC; VSPR