title: Genetically engineering Synechocystis sp. Pasteur Culture Collection 6803 for the sustainable production of the plant secondary metabolite p-coumaric acid.
abstract: p-Coumaric acid is the precursor of phenylpropanoids, which are plant secondary metabolites that are beneficial to human health. Tyrosine ammonia lyase catalyzes the production of p-coumaric acid from tyrosine. Because of their photosynthetic ability and biosynthetic versatility, cyanobacteria are promising candidates for the production of certain plant metabolites, including phenylpropanoids. Here, we produced p-coumaric acid in a strain of transgenic cyanobacterium Synechocystis sp. Pasteur Culture Collection 6803 (hereafter Synechocystis 6803). Whereas a strain of Synechocystis 6803 genetically engineered to express sam8, a tyrosine ammonia lyase gene from the actinomycete Saccharothrix espanaensis, accumulated little or no p-coumaric acid, a strain that both expressed sam8 and lacked slr1573, a native hypothetical gene shown here to encode a laccase that oxidizes polyphenols, produced ∼82.6 mg/L p-coumaric acid, which was readily purified from the growth medium.
authors.affiliation: Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR 72204; and.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR 72204; and.
authors.forename: Dan
authors.initials: D
authors.lastname: Cheng
authors.affiliation: Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR 72204; and.
authors.forename: Soumana
authors.initials: S
authors.lastname: Daddy
authors.affiliation: Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR 72204; andBiotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China qfhe@ualr.edu.
authors.forename: Qingfang
authors.initials: Q
authors.lastname: He
mesh_terms: D000642:Ammonia-Lyases / Q000737:chemistry / Q000235:genetics; D019149:Bioreactors; D053898:Biosynthetic Pathways / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000737:chemistry / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D018014:Gene Transfer Techniques; D005818:Genetic Engineering / Q000379:methods*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010957:Plasmids; D011422:Propionates; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046939:Synechocystis / Q000235:genetics* / Q000378:metabolism
chemical_list: D003373:Coumaric Acids; D017931:DNA Primers; D011422:Propionates; D000642:Ammonia-Lyases; C023208:L-tyrosine ammonia-lyase; C495469:p-coumaric acid