title: A simple optical configuration for cell tracking by dark-field microscopy.
abstract: We describe a simple optical configuration for dark-field microscopy at low magnification, realized with the use of standard microscope components. An inherent high contrast makes this method attractive for computer-assisted tracking and counting of microorganisms. We applied this setup for dark-field microscopy to measure the speed of migrating Dictyostelium amoebae.
authors.affiliation: Division of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10000 Zagreb, Croatia. Electronic address: vlatka.antolovic@irb.hr.
authors.forename: Vlatka
authors.initials: V
authors.lastname: Antolović
authors.affiliation: Division of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10000 Zagreb, Croatia. Electronic address: maja.marinovic@irb.hr.
authors.forename: Maja
authors.initials: M
authors.lastname: Marinović
authors.affiliation: Division of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10000 Zagreb, Croatia. Electronic address: vedrana.filic@irb.hr.
authors.forename: Vedrana
authors.initials: V
authors.lastname: Filić
authors.affiliation: Division of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10000 Zagreb, Croatia. Electronic address: iweber@irb.hr.
authors.forename: Igor
authors.initials: I
authors.lastname: Weber
mesh_terms: D002465:Cell Movement; D058948:Cell Tracking / Q000295:instrumentation / Q000379:methods*; D004023:Dictyostelium / Q000166:cytology*; D008853:Microscopy / Q000379:methods*
keywords: Annular aperture; Cell migration; Dark-field microscopy; Dictyostelium discoideum; Long-working-distance condenser; Tracking