title: Healing effect of sea buckthorn, olive oil, and their mixture on full-thickness burn wounds.
abstract: OBJECTIVE
  The purpose of this study is to evaluate the healing effect of silver sulfadiazine (SSD), sea buckthorn, olive oil, and 5% sea buckthorn and olive oil mixture on full-thickness burn wounds with respect to both gross and histopathologic features.
  
  
  METHODS
  Full-thickness burns were induced on 60 rats; the rats were then were divided into 5 groups and treated with sea buckthorn, olive oil, a 5% sea buckthorn/olive oil mixture, SSD, and normal saline (control). They were observed for 28 days, and the wounds' healing process was evaluated.
  
  
  RESULTS
  Wound contraction occurred faster in sea buckthorn, olive oil, and the sea buckthorn/olive oil mixture groups compared with the SSD and control groups. The volume of the exudates was controlled more effectively in wounds treated with the sea buckthorn/olive oil mixture. Purulent exudates were observed in the control group, but the others did not show infection. The group treated with sea buckthorn/olive oil mixture revealed more developed re-epithelialization with continuous basement membrane with a mature granulation tissue, whereas the SSD-treated group showed ulceration, necrosis, and immature granulation. The results show that sea buckthorn and olive oil individually are proper dressing for burn wounds and that they also show a synergetic effect when they are used together.
  
  
  CONCLUSIONS
  A sea buckthorn and olive oil mixture could be considered as an alternative dressing for full-thickness burns because of improved wound healing characteristics and antibacterial property.
authors.affiliation: Mitra Edraki, BSc, MSc, is an Instructor of Pediatric Nursing, Community-Based Psychiatric Care Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Armin Akbarzadeh is a medical student, Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran. Massood Hosseinzadeh, MD, is Assistant Professor of Pathology, Colorectal Research Center, Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran. Nader Tanideh, DVM, MPH, PhD, is Assistant Professor of Pharmacology, Stem Cell and Transgenic Technology Research Center & Pharmacology Department, Shiraz University of Medical Sciences, Shiraz, Iran. Alireza Salehi, MD, MPH, PhD, is Assistant Professor of Epidemiology, Research Center for Traditional Medicine and History of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Omid Koohi-Hosseinabadi, BSc, is a laboratory animal technician, Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Edraki
authors.forename: Armin
authors.initials: A
authors.lastname: Akbarzadeh
authors.forename: Massood
authors.initials: M
authors.lastname: Hosseinzadeh
authors.forename: Nader
authors.initials: N
authors.lastname: Tanideh
authors.forename: Alireza
authors.initials: A
authors.lastname: Salehi
authors.forename: Omid
authors.initials: O
authors.lastname: Koohi-Hosseinabadi
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001707:Biopsy, Needle; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D031281:Hippophae; D007150:Immunohistochemistry; D008297:Male; D000069463:Olive Oil; D064209:Phytochemicals / Q000494:pharmacology; D008517:Phytotherapy / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000069463:Olive Oil; D064209:Phytochemicals