title: Allicin protects rat cardiomyoblasts (H9c2 cells) from hydrogen peroxide-induced oxidative injury through inhibiting the generation of intracellular reactive oxygen species.
abstract: Oxidative stress is considered an important factor that promotes cell death in response to a variety of pathophysiological conditions. This study investigated the antioxidant properties of allicin, the principle ingredient of garlic, on preventing oxidative stress-induced injury. The antioxidant capacities of allicin were measured by using 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and hydrogen peroxide (H(2)O(2))-induced cell damage on H9c2 cardiomyoblasts. Allicin (0.3-10 μM) pre-incubation could concentration-dependently attenuate the intracellular reactive oxygen species (ROS) increase induced by H(2)O(2) on H9c2 cells. It could also protect H9c2 cells against H(2)O(2)-induced cell damage. However, the DPPH free radical scavenging activity of allicin was shown to be low. Therefore, it is believed that the protective effect of allicin on H9c2 cells could inhibit intracellular ROS production instead of scavenging extracellular H(2)O(2) or free radicals. For the observed protective effect on H9c2 cells, allicin might also be effective in reducing free radical-induced myocardial cell death in ischemic condition.
authors.affiliation: Department of Applied Biology and Chemical Technology, Food Safety and Technology Research Centre, The Hong Kong Polytechnic University , Hong Kong , China .
authors.forename: Jackie Yan-Yan
authors.initials: JY
authors.lastname: Chan
authors.forename: Hei-Tung
authors.initials: HT
authors.lastname: Tsui
authors.forename: Ivan Ying-Ming
authors.initials: IY
authors.lastname: Chung
authors.forename: Robbie Yat-Kan
authors.initials: RY
authors.lastname: Chan
authors.forename: Yiu-Wa
authors.initials: YW
authors.lastname: Kwan
authors.forename: Shun-Wan
authors.initials: SW
authors.lastname: Chan
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds; D002460:Cell Line; D004220:Disulfides; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D032386:Myoblasts, Cardiac / Q000187:drug effects*; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress; D010851:Picrates; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013441:Sulfinic Acids / Q000494:pharmacology*
chemical_list: D001713:Biphenyl Compounds; D004220:Disulfides; D016166:Free Radical Scavengers; D016877:Oxidants; D010851:Picrates; D017382:Reactive Oxygen Species; D013441:Sulfinic Acids; C006452:allicin; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Allicin; antioxidant; cardiovascular disease; health products; reactive oxygen species