title: Hydrogen-saturated saline protects intensive narrow band noise-induced hearing loss in guinea pigs through an antioxidant effect.
abstract: The purpose of the current study was to evaluate hydrogen-saturated saline protecting intensive narrow band noise-induced hearing loss. Guinea pigs were divided into three groups: hydrogen-saturated saline; normal saline; and control. For saline administration, the guinea pigs were given daily abdominal injections (1 ml/100 g) 3 days before and 1 h before narrow band noise exposure (2.5-3.5 kHz 130 dB SPL, 1 h). The guinea pigs in the control group received no treatment. The hearing function was assessed by the auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) recording. The changes of free radicals in the cochlea before noise exposure, and immediately and 7 days after noise exposure were also examined. By Scanning electron microscopy and succinate dehydrogenase staining, we found that pre-treatment with hydrogen-saturated saline significantly reduced noise-induced hair cell damage and hearing loss. We also found that the malondialdehyde, lipid peroxidation, and hydroxyl levels were significantly lower in the hydrogen-saturated saline group after noise trauma, indicating that hydrogen-saturated saline can decrease the amount of harmful free radicals caused by noise trauma. Our findings suggest that hydrogen-saturated saline is effective in preventing intensive narrow band noise-induced hearing loss through the antioxidant effect.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Wenzhou Medical College, Wenzhou, Zhejiang Province, China.
authors.forename: Longjun
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Daishi
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Lidong
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Mingbo
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Shiming
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Suoqiang
authors.initials: S
authors.lastname: Zhai
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001309:Auditory Threshold / Q000187:drug effects / Q000502:physiology; D003051:Cochlea / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology; D005260:Female; D006168:Guinea Pigs; D006317:Hearing Loss, Noise-Induced / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D006859:Hydrogen / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors / Q000378:metabolism; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000037:antagonists & inhibitors / Q000378:metabolism; D017084:Otoacoustic Emissions, Spontaneous / Q000502:physiology; D020011:Protective Agents / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D017665:Hydroxyl Radical; D012965:Sodium Chloride; D008315:Malondialdehyde; D006859:Hydrogen; D013385:Succinate Dehydrogenase