title: Processing bodies accumulate in human cytomegalovirus-infected cells and do not affect viral replication at high multiplicity of infection.
abstract: Translationally silenced mRNAs are recruited to two major classes of RNA granules in the cytoplasm, processing bodies (PBs) and stress granules (SGs). We show that PBs accumulated after human cytomegalovirus (HCMV) infection. PB assembly after HCMV infection was also detected in the presence of the protein synthesis inhibitor, cycloheximide, but required active RNA synthesis. UV-inactivated HCMV virions were sufficient to induce PB accumulation in HFF cells treated with cycloheximide. Viral IE1 RNA did not colocalize with PBs, and we could not detect an effect of PB accumulation on viral growth. These results may indicate that HCMV inhibits the colocalization of IE1 mRNA with PBs, preventing IE1 mRNA decay and translational inhibition.
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Seto
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan; Medicine and Molecular Science, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
authors.forename: Teruki
authors.initials: T
authors.lastname: Inoue
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakatani
authors.affiliation: Medicine and Molecular Science, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan. Electronic address: hisomura@gunma-u.ac.jp.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Isomura
mesh_terms: D000914:Antibodies, Viral; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000502:physiology*; D004279:DNA, Viral / Q000502:physiology; D005347:Fibroblasts / Q000821:virology*; D006801:Humans; D014779:Virus Replication / Q000502:physiology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral
keywords: Eukaryotic initiation factor; Human cytomegalovirus; MRNA decay; P-body; Stress granule; Translation; Translation initiation