title: Effect of EPH-ephrin signaling on the growth of human leukemia cells.
abstract: BACKGROUND
  Signaling induced by binding of erythropoietin-producing hepatoma-amplified sequence (EPH) receptors to their cell-surface ephrin ligands is implicated in hematopoiesis and growth of various cancer cells. However, the roles of EPH-ephrin signaling in leukemia have not been elucidated. We investigated the effects of EPHB4 and ephrin B2 on the growth of leukemia cells.
  
  
  METHODS
  Seven human leukemia cell lines were used to examine the effects of recombinant ephrin B2 and EPHB4 on cell proliferation by colorimetric WST-1 assay and colony assays; on protein tyrosine phosphorylation; and on mRNA expression by reverse transcription-polymerase chain reaction and microarray analysis.
  
  
  RESULTS
  In an erythroid leukemia-derived cell line AA, exogenous ephrin B2 induced proliferation and colony formation; in addition, it up-regulated protein tyrosine phosphorylation and the expression of growth-related genes such as FBJ murine osteosarcoma viral oncogene homolog B and v-src avian sarcoma viral oncogene homolog.
  
  
  CONCLUSIONS
  Growth-promoting effects of ephrin B2 were observed in an erythroid leukemia cell line, suggesting that the EPH-ephrin signaling may be involved in the pathology of leukemia.
authors.affiliation: Department of Laboratory Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Department of Laboratory Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Itoh
authors.affiliation: Department of Laboratory Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nara
authors.affiliation: Department of Laboratory Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan tohda.mlab@tmd.ac.jp.
authors.forename: Shuji
authors.initials: S
authors.lastname: Tohda
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D015153:Blotting, Western; D049109:Cell Proliferation; D036342:Ephrins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D036081:Receptors, Eph Family / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D036342:Ephrins; D012333:RNA, Messenger; D036081:Receptors, Eph Family
keywords: EPH receptor; ephrin; leukemia; notch