title: Activation of the TLR1/2 pathway induces the shaping of the immune response status of peripheral blood leukocytes.
abstract: Toll-like receptors (TLRs) play an essential role in the activation and regulation of the innate and adaptive immune responses through the recognition of specific components of pathogens. TLR1/2 on the cell surface plays an important role in defending against Gram-positive bacteria. The aim of the present study was to examine the expressional variation of immunomodulatory molecules in peripheral blood leukocytes (PBLs) treated with the TLR1/2 agonist, Pam3Cys. The quantitative polymerase chain reaction result showed dramatically increased expression of immune-related factors treated with Pam3Cys. Antibody-chip assays confirmed that activation of TLR1/2 could induce secretion of four important immune factors [interleukin (IL)-6, IL-8, macrophage inflammatory protein-1α and interferon-β). Western-blot analysis indicated the upregulation of three significant signal kinase proteins (phosphorylated signal transducer and activator of transcription 3, extracellular signal-related kinase and c-Jun N-terminal kinase 2). The study demonstrated that there were numerous molecules involved in the immune response of PBLs stimulated by the TLR1/2 ligand. Our future studies will focus on the mechanisms of these molecules in the TLR1/2 agonist-mediated immune response.
authors.affiliation: Department of Cardiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Laboratory of Pathology, Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
keywords: Toll-like receptor 1/2; agonist; immunomodulatory molecules; peripheral blood leukocyte