title: Determination of cephalomannine in rat plasma by gradient elution UPLC-MS/MS method.
abstract: A rapid, sensitive and selective ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of cephalomannine in rat plasma. Sample preparation was accomplished through a simple one-step deproteinization procedure with 0.2 mL of perchloric acid-methanol (1:9, v/v) to a 0.1 mL plasma sample. Plasma samples were separated by UPLC on an Acquity UPLC BEH C18 column using a mobile phase consisting of acetonitrile-0.1% formic acid in water with gradient elution. The total run time was 2.0 min and the elution of cephalomannine was at 1.60 min. The detection was performed on a triple quadrupole tandem mass spectrometer in the multiple reaction-monitoring (MRM) mode using the respective transitions m/z 832.8→264.1 for cephalomannine and m/z 812.6→286.0 for 10-DAT (internal standard), respectively. The calibration curve was linear over the range of 10-2,000 ng/mL with a lower limit of quantitation (LLOQ) of 10 ng/mL. Mean recovery of cephalomannine in plasma was in the range of 80.9-85.3%. Intra-day and inter-day precision were both <11.2%. This method was successfully applied in pharmacokinetic study after intravenous administration of 5.0mg/kg cephalomannine in rats.
authors.affiliation: Department of Oncology, Cancer Institute, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Xin-Shuai
authors.initials: XS
authors.lastname: Wang
authors.affiliation: Department of Oncology, Cancer Institute, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Jia-Chun
authors.initials: JC
authors.lastname: Sun
authors.affiliation: Department of Oncology, Cancer Institute, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Rui-Na
authors.initials: RN
authors.lastname: Yang
authors.affiliation: Department of Oncology, Cancer Institute, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Oncology, Cancer Institute, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Liang
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Ying-Fei
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Department of Oncology, Cancer Institute, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, PR China. Electronic address: gsg112258@163.com.
authors.forename: She-Gan
authors.initials: SG
authors.lastname: Gao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D043823:Taxoids / Q000097:blood*
chemical_list: D043823:Taxoids; C056348:cephalomannine
keywords: Cephalomannine; Pharmacokinetics; Rat plasma; UPLC–MS/MS