title: Artificial oxidative stress-tolerant Corynebacterium glutamicum.
abstract: We have reported a transcription profile of an adapted Corynebacterium glutamicum that showed enhanced oxidative stress resistance. To construct an artificial oxidative stress-resistant strain, gene clusters in the β-ketoadipate pathway, which were up-regulated in the adapted strain, were artificially expressed in the wild-type C. glutamicum. The wild-type strain was unable to grow under 2 mM H2O2 containing minimal medium, while the strains expressing pca gene clusters restored growth under the same medium, and the pcaHGBC expression showed the most significant effect among the gene clusters. The expressions of pca gene clusters also enabled the wild-type to increase its resistance against oxidative stressors, such as diamide and cumene hydroperoxide, as well as H2O2. The oxidative stress tolerance of the strain was correlated to the reactive oxygen species (ROS)-scavenging activity of the cell extract. The reason for the enhanced oxidative stress-resistance of C. glutamicum and its applications on the synthetic strain development are discussed.
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, Bucheon 420-743, Gyeonggi, Korea.
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, Bucheon 420-743, Gyeonggi, Korea.
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, Bucheon 420-743, Gyeonggi, Korea ; Present address: ST Pharm Co., Siheung 429-912, Gyeonggi, Korea.
authors.forename: Jiyoon
authors.initials: J
authors.lastname: Seo
authors.affiliation: Department of Biological Engineering, Inha University, Inchon 402-751, Korea.
authors.forename: Eung-Soo
authors.initials: ES
authors.lastname: Kim
authors.affiliation: Department of Biotechnology and Bioinformatics, Korea University, Jochiwon, Chungnam 339-700, Korea.
authors.forename: Heung-Shick
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, Bucheon 420-743, Gyeonggi, Korea.
authors.forename: Pil
authors.initials: P
authors.lastname: Kim
keywords: Corynebacterium glutamicum; Oxidative stress-tolerance; pca gene clusters; β-ketoadipate pathway