title: Low-dose recombinant human bone morphogenetic protein-2 to enhance the osteogenic potential of the Schneiderian membrane in the early healing phase: in vitro and in vivo studies.
abstract: OBJECTIVE
  The objectives of this study were to confirm the osteogenic potential of the Schneiderian membrane and to elucidate the early healing pattern of low-dose recombinant human bone morphogenetic protein-2 (rhBMP-2)-coated biphasic calcium phosphate (BCP).
  
  
  METHODS
  The osteogenic potential of the Schneiderian membrane and enhancement by rhBMP-2 were evaluated by in vitro analysis. RhBMP-2-coated BCP (experimental group) and BCP soaked with saline (control group) were applied to the maxillary sinus in rabbits. After 2 weeks, micro-computed tomographic and histometric analyses were performed.
  
  
  RESULTS
  Enhanced osteogenic potential was found when cells from the Schneiderian membrane were treated with rhBMP-2. Micro-computed tomographic analysis showed that the total augmented volume was significantly larger in the experimental group. Different healing patterns were observed in 3 regions, although the area of new bone did not differ significantly. Although more newly formed bone appeared, particularly along the Schneiderian membrane in the experimental group, the difference was not statistically significant.
  
  
  CONCLUSIONS
  RhBMP-2 enhanced the osteogenic potential of the Schneiderian membrane in vitro. However, low-dose rhBMP-2-coated BCP failed to exert a statistically significant effect in vivo, although it appeared to be effective in sinus augmentation specifically for the volumetric increase in the early phase.
authors.affiliation: Clinical Research Assistant Professor, Department of Periodontology, College of Dentistry, Yonsei University, Seoul, South Korea.
authors.forename: Min-Soo
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Graduate Student, Department of Periodontology, College of Dentistry, Yonsei University, Seoul, South Korea.
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Kwon
authors.affiliation: Clinical Assistant Professor, Department of Periodontology, College of Dentistry, Yonsei University, Seoul, South Korea.
authors.forename: Jung-Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Associate Professor, Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, South Korea.
authors.forename: Je Seon
authors.initials: JS
authors.lastname: Song
authors.affiliation: Professor, Department of Periodontology, College of Dentistry, Yonsei University, Seoul, South Korea.
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Associate Professor, Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, South Korea. Electronic address: drjew@yuhs.ac.
authors.forename: Ui-Won
authors.initials: UW
authors.lastname: Jung
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009297:Nasal Mucosa / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*; D055114:X-Ray Microtomography
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins