title: N-glycoprotein surfaceomes of four developmentally distinct mouse cell types.
abstract: OBJECTIVE
  Detailed knowledge of cell surface proteins present during early embryonic development remains limited for most cell lineages. Due to the relevance of cell surface proteins in their functional roles controlling cell signaling and their utility as accessible, nongenetic markers for cell identification and sorting, the goal of this study was to provide new information regarding the cell surface proteins present during early mouse embryonic development.
  
  
  METHODS
  Using the cell surface capture technology, the cell surface N-glycoproteomes of three cell lines and one in vitro differentiated cell type representing distinct cell fates and stages in mouse embryogenesis were assessed.
  
  
  RESULTS
  Altogether, more than 600 cell surface N-glycoproteins were identified represented by >5500 N-glycopeptides.
  
  
  CONCLUSIONS
  The development of new, informative cell surface markers for the reliable identification and isolation of functionally defined subsets of cells from early developmental stages will advance the use of stem cell technologies for mechanistic developmental studies, including disease modeling and drug discovery.
authors.affiliation: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Erin M
authors.initials: EM
authors.lastname: Kropp
authors.forename: Subarna
authors.initials: S
authors.lastname: Bhattacharya
authors.forename: Matthew
authors.initials: M
authors.lastname: Waas
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Chuppa
authors.forename: Anna-Katerina
authors.initials: AK
authors.lastname: Hadjantonakis
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Boheler
authors.forename: Rebekah L
authors.initials: RL
authors.lastname: Gundry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D047108:Embryonic Development; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D040901:Proteomics
chemical_list: D006023:Glycoproteins
keywords: Biomarkers; Cardiovascular; Glycoproteins; Glycoproteomics; Plasma membrane