title: Characterization of microsatellite markers developed from Prosopis rubriflora and Prosopis ruscifolia (Leguminosae - Mimosoideae), legume species that are used as models for genetic diversity studies in Chaquenian areas under anthropization in South America.
abstract: BACKGROUND
  Prosopis rubriflora and Prosopis ruscifolia are important species in the Chaquenian regions of Brazil. Because of the restriction and frequency of their physiognomy, they are excellent models for conservation genetics studies. The use of microsatellite markers (Simple Sequence Repeats, SSRs) has become increasingly important in recent years and has proven to be a powerful tool for both ecological and molecular studies.
  
  
  RESULTS
  In this study, we present the development and characterization of 10 new markers for P. rubriflora and 13 new markers for P. ruscifolia. The genotyping was performed using 40 P. rubriflora samples and 48 P. ruscifolia samples from the Chaquenian remnants in Brazil. The polymorphism information content (PIC) of the P. rubriflora markers ranged from 0.073 to 0.791, and no null alleles or deviation from Hardy-Weinberg equilibrium (HW) were detected. The PIC values for the P. ruscifolia markers ranged from 0.289 to 0.883, but a departure from HW and null alleles were detected for certain loci; however, this departure may have resulted from anthropic activities, such as the presence of livestock, which is very common in the remnant areas.
  
  
  CONCLUSIONS
  In this study, we describe novel SSR polymorphic markers that may be helpful in future genetic studies of P. rubriflora and P. ruscifolia.
authors.forename: Fábio M
authors.initials: FM
authors.lastname: Alves
authors.forename: Maria I
authors.initials: MI
authors.lastname: Zucchi
authors.forename: Ana M G
authors.initials: AM
authors.lastname: Azevedo-Tozzi
authors.forename: Angela L B
authors.initials: AL
authors.lastname: Sartori
authors.affiliation: Departamento de Biologia Vegetal (BV), Instituto de Biologia, Universidade Estadual de Campinas - UNICAMP, Cidade Universitária Zeferino Vaz, CP 6109, CEP 13083-970 Campinas, SP, Brazil. anete@unicamp.br.
authors.forename: Anete P
authors.initials: AP
authors.lastname: Souza
mesh_terms: D000483:Alleles; D001938:Brazil; D003247:Conservation of Natural Resources; D017931:DNA Primers; D056426:Genetic Loci; D005838:Genotype; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D029862:Prosopis / Q000145:classification / Q000235:genetics*
chemical_list: D017931:DNA Primers