title: [Effect of curcumin on radiosensitization of CNE-2 cells and its mechanism].
abstract: OBJECTIVE
  To investigate the effect of curcumin (Cur) on radiosensitivity of nasopharyngeal carcinoma cell CNE-2 and its mechanism.
  
  
  METHODS
  The effect of curcumin on radiosensitivity was determined by the clone formation assay. The cell survival curve was fitted by Graph prism 6. 0. The changes in cell cycle were analyzed by flow cytometry (FCM). The differential expression of long non-coding RNA was detected by gene chip technology. Part of differentially expressed genes was verified by Real-time PCR.
  
  
  RESULTS
  After 10 micro mol L-1 Cur had worked for 24 h, its sensitization enhancement ratio was 1. 03, indicating that low concentration of curcumin could increase the radiosensitivity of nasopharyngeal carcinoma cells; FCM displayed a significant increase of G2 phase cells and significant decrease of S phase cells in the Cur combined radiation group. In the Cur group, the GUCY2GP, H2BFXP, LINC00623 IncRNA were significantly up-regulated and ZRANB2-AS2 LOC100506835, FLJ36000 IncRNA were significantly down-regulated.
  
  
  CONCLUSIONS
  Cur has radiosensitizing effect on human nasopharyngeal carcinoma CNE-2 cells. Its mechanism may be related to the changes in the cell cycle distribution and the expression of long non-coding IncRNA.
authors.forename: Qi-Rui
authors.initials: QR
authors.lastname: Wang
authors.forename: Hao-Ning
authors.initials: HN
authors.lastname: Fan
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Yin
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Cai
authors.forename: Meng
authors.initials: M
authors.lastname: Shao
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Diao
authors.forename: Yuan-Liang
authors.initials: YL
authors.lastname: Liu
authors.forename: Xue-Gang
authors.initials: XG
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Tong
authors.forename: Qin
authors.initials: Q
authors.lastname: Fan
mesh_terms: D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003474:Curcumin / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D062085:RNA, Long Noncoding / Q000235:genetics; D011836:Radiation Tolerance / Q000187:drug effects*
chemical_list: D062085:RNA, Long Noncoding; D003474:Curcumin