title: MicroRNA-377 inhibited proliferation and invasion of human glioblastoma cells by directly targeting specificity protein 1.
abstract: BACKGROUND
  Increasing evidence has indicated that microRNAs (miRNAs) are strongly implicated in the initiation and progression of glioblastoma multiforme (GBM). Here, we identiﬁed a novel tumor suppressive miRNA, miR-377, and investigated its role and therapeutic effect for GBM.
  
  
  METHODS
  MiRNA global screening was performed on GBM patient samples and adjacent nontumor brain tissues. The expression of miR-377 was detected by real-time reverse-transcription PCR. The effects of miR-377 on GBM cell proliferation, cell cycle progression, invasion, and orthotopic tumorigenicity were investigated The therapeutic effect of miR-377 mimic was explored in a subcutaneous GBM model. Western blot and luciferase reporter assay were used to identify the direct and functional target of miR-377.
  
  
  RESULTS
  MiR-377 was markedly downregulated in human GBM tissues and cell lines. Overexpression of miR-377 dramatically inhibited cell growth both in culture and in orthotopic xenograft tumor models, blocked G1/S transition, and suppressed cell invasion in GBM cells. Importantly, introduction of miR-377 could strongly inhibit tumor growth in a subcutaneous GBM model. Subsequent investigation revealed that specificity protein 1 (Sp1) was a direct and functional target of miR-377 in GBM cells. Silencing of Sp1 recapitulated the antiproliferative and anti-invasive effects of miR-377, whereas restoring the Sp1 expression antagonized the tumor-suppressive function of miR-377. Finally, analysis of miR-377 and Sp1 levels in human GBM tissues revealed that miR-377 is inversely correlated with Sp1 expression.
  
  
  CONCLUSIONS
  These findings reveal that miR-377/Sp1 signaling that may be required for GBM development and may consequently serve as a therapeutic target for the treatment of GBM.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Hui
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Wanghao
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Zhengxin
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Jingmin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Wenting
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Shu-Yu
authors.initials: SY
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Shuying
authors.initials: S
authors.lastname: Shen
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Yaxuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Daru
authors.initials: D
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Shiguang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Yongping
authors.initials: Y
authors.lastname: You
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (R.Z., H.L., W.C., Z.C., Q.D., Y.Z., Y.Y., N.L., H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China (H-W.W.); State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes for Biomedical Sciences, Fudan University, Shanghai, China (Y.C., J.Y., D.L.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Beyster Center for Genomics of Psychiatric Diseases, Department of Psychiatry, University of California San Diego, La Jolla, California (W.W.); School of Radiation Medicine and Protection, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China (S-Y.Z.); Institute of Biochemistry, Zhejiang University, Hangzhou, China (S.S.); Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China (T.J.); Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, China (S.Z.); Chinese Glioma Cooperative Group (T.J., Y.Y., N.L., H.W.).
authors.forename: Huibo
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: C534063:MIRN377 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D016329:Sp1 Transcription Factor
keywords: Sp1; glioblastoma multiforme; invasion; miR-377; proliferation