title: Analysis of cell cycle shortening and developmental interruption in Dictyostelium discoideum allC RNAi mutants.
abstract: Dictyostelium discoideum allC RNAi mutant cells are motile and aggregate together, but do not undergo further morphological development. The relatively quick growth rate of allC RNAi mutants compared to wild-type D. discoideum results in a shortened mutant cell cycle. However, at present, little is known about the mechanism underlying this phenomenon. Here, we used semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), real-time quantitative RT-PCR, two-dimensional gel electrophoresis, and mass spectrometry/mass spectrometry to elucidate the phenomenon. We found significant downregulation of myosin II heavy chain, D. discoideum calcium-dependent cell adhesion molecule-1 (DdCAD-1) mRNA, DdCAD-1 protein, D. discoideum mRNA for 14-3-3 and 14-3-3 protein, and type A von Willebrand factor domain-containing protein mRNA in allC RNAi mutants. The results suggest that downregulation of the myosin II heavy chain could be one of key factors causing the developmental interruption and that downregulation of the 14-3-3 protein and the type A von Willebrand factor domain-containing protein mRNA plays an important role in shortening the cell cycle of allC RNAi mutants.
authors.affiliation: School of Life Science, East China Normal University, Shanghai, China.
authors.forename: D M
authors.initials: DM
authors.lastname: Xue
authors.affiliation: School of Life Science, East China Normal University, Shanghai, China lshou@bio.ecnu.edu.cn.
authors.forename: L S
authors.initials: LS
authors.lastname: Hou
authors.affiliation: School of Life Science, East China Normal University, Shanghai, China.
authors.forename: S R
authors.initials: SR
authors.lastname: Zhang
authors.affiliation: School of Life Science, East China Normal University, Shanghai, China.
authors.forename: N X
authors.initials: NX
authors.lastname: Chen
mesh_terms: D048948:14-3-3 Proteins / Q000096:biosynthesis / Q000235:genetics*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002449:Cell Aggregation / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D004023:Dictyostelium; D005786:Gene Expression Regulation / Q000235:genetics; D009154:Mutation; D024462:Myosin Type II / Q000096:biosynthesis; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D014841:von Willebrand Factor / Q000096:biosynthesis
chemical_list: D048948:14-3-3 Proteins; D002135:Calcium-Binding Proteins; D015815:Cell Adhesion Molecules; C100604:DdCAD-1 protein, Dictyostelium discoideum; D012333:RNA, Messenger; D014841:von Willebrand Factor; D024462:Myosin Type II