title: Innate immune response of Indian major carp, Labeo rohita infected with oomycete pathogen Aphanomyces invadans.
abstract: The fish pathogenic oomycete Aphanomyces invadans is the causative agent of epizootic ulcerative syndrome (EUS), a fish disease of international significance and reportable to the World Organisation for Animal Health. In spite of the current and potential impact of A. invadans infection on fisheries and aquaculture sectors of the world, very little is known about the host-A. invadans interactions. In the present study, following experimental infection with A. invadans in one of the Indian major carps, Labeo rohita, sequential changes in various innate immune parameters were monitored. The results indicated that at early stages of infection, no significant changes in any of the studied innate immune parameters were observed. However, at the advanced stages of infection from 6 to 12 days post infection (dpi), the respiratory burst and alternate complement activity were significantly higher whereas lysozyme, antiproteases and α-2 macroglobulin values were significantly lower than the control group and also from the infected group at earlier stages of infection. Since, the possibility of vaccination of fish against A. invadans appears remote due to difficulties in eliciting a specific antibody response, the information generated in the present study could be useful for developing strategies for improving resistance to A. invadans infection by stimulating the innate immunity through immunomodulation.
authors.affiliation: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, Uttar Pradesh, India.
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Yadav
authors.affiliation: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, Uttar Pradesh, India. Electronic address: pradhanpk1@gmail.com.
authors.forename: Pravata K
authors.initials: PK
authors.lastname: Pradhan
authors.affiliation: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, Uttar Pradesh, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Sood
authors.affiliation: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, Uttar Pradesh, India.
authors.forename: Dharmendra K
authors.initials: DK
authors.lastname: Chaudhary
authors.affiliation: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, Uttar Pradesh, India.
authors.forename: Dev K
authors.initials: DK
authors.lastname: Verma
authors.affiliation: ICAR Research Complex for North Eastern Hill Region, Lambucherra 799 210, Tripura, India.
authors.forename: Chandan
authors.initials: C
authors.lastname: Debnath
authors.affiliation: ICAR Research Complex for North Eastern Hill Region, Lambucherra 799 210, Tripura, India.
authors.forename: Lopamudra
authors.initials: L
authors.lastname: Sahoo
authors.affiliation: School of Environmental Biology, Awadesh Pratap Singh University, Rewa 486 003, Madhya Pradesh, India.
authors.forename: U K
authors.initials: UK
authors.lastname: Chauhan
authors.affiliation: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, Uttar Pradesh, India.
authors.forename: Peyush
authors.initials: P
authors.lastname: Punia
authors.affiliation: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, Uttar Pradesh, India.
authors.forename: Joy K
authors.initials: JK
authors.lastname: Jena
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D044744:Aphanomyces / Q000276:immunology*; D002347:Carps; D003170:Complement Pathway, Alternative / Q000276:immunology; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D007113:Immunity, Innate / Q000276:immunology*; D007239:Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D009113:Muramidase / Q000378:metabolism; D011480:Protease Inhibitors / Q000378:metabolism; D016897:Respiratory Burst / Q000276:immunology; D012709:Serum Albumin; D012712:Serum Globulins
chemical_list: D011480:Protease Inhibitors; D012709:Serum Albumin; D012712:Serum Globulins; D009113:Muramidase
keywords: Aphanomyces invadans; Innate immunity; Labeo rohita; Oomycete