title: Matrilin-3 as a putative effector of C-type natriuretic peptide signaling during TGF-β induced chondrogenic differentiation of mesenchymal stem cells.
abstract: C-type natriuretic peptide (CNP) signaling has been implicated as an important regulator of chondrogenic differentiation during endochondral bone development. This preliminary study further investigated the putative effectors and/or targets of CNP signaling in transforming growth factor (TGF)-β induced in vitro chondrogenic differentiation of mesenchymal stem cells (MSCs). Previously characterized human trabecular bone derived MSCs were induced either with only TGF-β1 or with a combination of TGF-β1 and CNP in micromass culture for 10 or 20 days. Genome wide gene expression profile changes in between these two groups were analyzed on day-10 or day-20 of culture. Results revealed that there were only 7 genes, whose expression change was fourfolds or higher in TGF-β1 and CNP fed group in comparison to only TGF-β1 fed group. The up-regulated genes included matrilin-3 (MATN3), engulfment and cell motility 1 (ELMO1), CD24, and DCN1, defective in cullin neddylation 1, domain containing 1 (DCUN1D1). The down-regulated genes, on the other hand, included LIM domain kinase 2 (LIMK2), Ewing sarcoma breakpoint region 1, and guanine nucleotide binding protein (G protein), gamma 12 (GNG12). The up-regulation of MATN3 was confirmed on the basis of RT-PCR. The known literature on both CNP signaling and MATN3 function in chondrogenesis match with each other and suggest MATN3 as a putative effector and/or target of CNP signaling during this process.
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering and Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada.
authors.forename: Mustafa Ege
authors.initials: ME
authors.lastname: Babadagli
authors.forename: Berna
authors.initials: B
authors.lastname: Tezcan
authors.forename: Seda Tasir
authors.initials: ST
authors.lastname: Yilmaz
authors.forename: A Cevik
authors.initials: AC
authors.lastname: Tufan
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism; D051927:CD24 Antigen / Q000235:genetics / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D020219:Chondrogenesis / Q000235:genetics; D016000:Cluster Analysis; D019295:Computational Biology; D015536:Down-Regulation; D044388:GTP-Binding Protein gamma Subunits / Q000235:genetics / Q000378:metabolism; D006801:Humans; D054461:Lim Kinases / Q000235:genetics / Q000378:metabolism; D064235:Matrilin Proteins / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D046228:Microarray Analysis; D020098:Natriuretic Peptide, C-Type / Q000494:pharmacology*; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051927:CD24 Antigen; C497889:CD24 protein, human; D002148:Calmodulin-Binding Proteins; C465214:ELMO1 protein, human; C500986:EWSR1 protein, human; D044388:GTP-Binding Protein gamma Subunits; D064235:Matrilin Proteins; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins; D053773:Transforming Growth Factor beta1; D020098:Natriuretic Peptide, C-Type; C102895:LIMK2 protein, human; D054461:Lim Kinases