title: Extraction and purification of polysaccharides from pine medicinal mushroom, Tricholoma matsutake (higher Basidiomycetes) fruit bodies.
abstract: Ultrasound-assisted extraction, purification, and characterization of antioxidant polysaccharides from Tricholoma matsutake were carried out. On the basis of the results of a single-factor test, three independent and main variables, including extraction time (X1: 130-170 s), ultrasonic power (X2: 340-380 W), and ratio of water to raw material (X3: 45-55 mL/g) were studied by Box-Behnken design. The optimum extraction conditions are as follows: extraction time 160 s, ultrasonic power 365 W, and ratio of water to raw material 53.5 mL/g. Under the optimized conditions, the yield of T. matsutake polysaccharides (TMP) was 7.97±0.31%. The crude TMP was purified by DEAE-cellulose 52 chromatography and Sephadex G-100 chromatography to afford two fractions, TMP-1 and TMP-2. The crude TMP contained 85.76% carbohydrates, 3.57% proteins, and 0.13% uronic acids. The constituent monosaccharides were predominantly glucose, galactose, mannose, xylose, fucose, and glucuronic acid residues.
authors.affiliation: Huaiyin Institute of Technology, School of Life Science and Chemical Engineering, Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration;Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaian 223300.
authors.forename: Qinghong
authors.initials: Q
authors.lastname: You
authors.affiliation: Huaiyin Institute of Technology, School of Life Science and Chemical Engineering, Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaian 223001, China; School of Food and Biological Engineering, Jiangsu University.
authors.forename: Xiulian
authors.initials: X
authors.lastname: Yin
authors.affiliation: Huaiyin Institute of Technology, School of Life Science and Chemical Engineering, Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaian 223001, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Huaiyin Institute of Technology, School of Life Science and Chemical Engineering, Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaian 223001, China.
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D055439:Tricholoma / Q000737:chemistry*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides