title: Effects of agmatine and resveratrol on RGC-5 cell behavior under light stimulation.
abstract: A light radiation causes dysfunction and death of retinal cells and leads to degeneration. Present study, investigated the light-induced cell dysfunction, and their activity. Further, the effects of agmatine and resveratrol on light-induced damage and these underlying photo-oxidative and protective mechanisms were monitored by real-time bio-impedance system. After light exposure retinal ganglion cells underwent death in a time dependent manner. During light exposure the cells elevate free radicals and Ca(2+), followed by nitric oxide (NO) and tumor necrosis factor-α (TNF-α), which can be facilitated to cell demise. The results revealed that these drugs can control the elevation of free radical, calcium gating, NO level, and increased TNF-α, which could diminish cell photo-damage. In summary, resveratrol helps more to rescue damaged cells compared to agmatine. The proposed system suggested mechanism could meet to identify the photo-toxic effects in retinal cells, and provides high throughput screening for early stages photo-damage.
authors.affiliation: Department of Bionanotechnology, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do 461-701, Republic of Korea. Electronic address: devasier@gmail.com.
authors.forename: Devasier
authors.initials: D
authors.lastname: Bennet
authors.affiliation: Department of Bionanotechnology, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do 461-701, Republic of Korea; Graduate Gachon Medical Research Institute, Gil Medical Center, Inchon 405-760, Republic of Korea. Electronic address: samkim@gachon.ac.kr.
authors.forename: Sanghyo
authors.initials: S
authors.lastname: Kim
mesh_terms: D000376:Agmatine / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D008027:Light; D009569:Nitric Oxide / Q000378:metabolism; D010775:Photic Stimulation; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000528:radiation effects*; D013267:Stilbenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D020011:Protective Agents; D017382:Reactive Oxygen Species; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D000376:Agmatine; D000077185:Resveratrol; D002118:Calcium
keywords: Impedance-based cell study; Photo-protective agents; Photo-toxicity; Reactive oxygen species; Tumor necrosis factor-α