title: Target cell extraction coupled with LC-MS/MS analysis for screening potential bioactive components in Ginkgo biloba extract with preventive effect against diabetic nephropathy.
abstract: A rapid and useful approach for screening potential bioactive components in Ginkgo biloba extract (GBE) with preventive effect against diabetic nephropathy (DN) was developed using mesangial cells extraction coupled with high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. Mesangial cells were first divided into two groups according to their treatments with high glucose or high glucose plus GBE. After incubation for 4, 8, 12, 16, 24 and 48 h, the cells were harvested and extracted with 40% acetic acid in water before LC-MS/MS analysis. Then, 19 compounds and five metabolites were found to selectively combine with mesangial cells. Notably, compounds including quercetin and rutin were identified or tentatively characterized according to the results of retention time and MS spectra, which is highly consistent with our previous reports that quercetin and rutin are potent protective agents against glomerulosclerosis in DN. Therefore, all these results indicate that target cell extraction coupled with LC-MS/MS analysis can be successfully applied for predicting the bioactive components in GBE with preventive effect against DN.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical College, Xuzhou, 221004, China; Key Laboratory of New Drug and Clinical Application, Xuzhou Medical College, Xuzhou, 221004, China.
authors.forename: Jing-ying
authors.initials: JY
authors.lastname: Qiu
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.forename: Xiao-xiao
authors.initials: XX
authors.lastname: Zheng
authors.forename: Xiang-lan
authors.initials: XL
authors.lastname: Jiang
authors.forename: Dong-zhi
authors.initials: DZ
authors.lastname: Yang
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Yu
authors.forename: Qian
authors.initials: Q
authors.lastname: Du
authors.forename: Dao-quan
authors.initials: DQ
authors.lastname: Tang
authors.forename: Xiao-xing
authors.initials: XX
authors.lastname: Yin
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D003928:Diabetic Nephropathies / Q000188:drug therapy; D020441:Ginkgo biloba / Q000737:chemistry*; D006801:Humans; D050527:Mesangial Cells / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010936:Plant Extracts
keywords: Ginkgo biloba extract; LC-MS/MS; bioactive components; target cell extraction