title: Yeast diversity associated to sediments and water from two Colombian artificial lakes.
abstract: In Colombia, knowledge of the yeast and yeast-like fungi community is limited because most studies have focused on species with clinical importance. Sediments and water represent important habitats for the study of yeast diversity, especially for yeast species with industrial, biotechnological, and bioremediation potential. The main purpose of this study was to identify and compare the diversity of yeast species associated with sediment and water samples from two artificial lakes in Universidad del Valle (Cali-Colombia). Yeast samplings were performed from fifteen sediment samples and ten water samples. Grouping of similar isolates was initially based on colony and cell morphology, which was then complemented by micro/mini satellite primed PCR banding pattern analysis by using GTG5 as single primer. A representative isolate for each group established was chosen for D1/D2 domain sequencing and identification. In general, the following yeast species were identified: Candida albicans, Candida diversa, Candida glabrata, Candida pseudolambica, Cryptococcus podzolicus, Cryptococcus rajasthanensis, Cryptococcus laurentii, Williopsis saturnus, Hanseniaspora thailandica, Hanseniaspora uvarum, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, Torulaspora delbrueckii, Torulaspora pretoriensis, Tricosporon jirovecii, Trichosporon laibachii and Yarrowia lypolitica. Two possible new species were also found, belonging to the Issatchenkia sp. and Bullera sp. genera. In conclusion, the lakes at the Universidad del Valle campus have significant differences in yeast diversity and species composition between them.
authors.affiliation: Grupo de Investigación en Biología Molecular de Microorganismos Departamento de Biología Universidad del Valle Cali Colombia.
authors.forename: L M
authors.initials: LM
authors.lastname: Silva-Bedoya
authors.affiliation: Grupo de Investigación en Biología Molecular de Microorganismos Departamento de Biología Universidad del Valle Cali Colombia ; Programa de Pós-Graduação em Biologia Celular e Molecular Centro de Biotecnologia Universidade Federal do Rio Grande do Sul Porto AlegreRS Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Ramírez-Castrillón
authors.affiliation: Grupo de Investigación en Biología Molecular de Microorganismos Departamento de Biología Universidad del Valle Cali Colombia ; Departmento de Biología Universidad del Valle Cali Colombia.
authors.forename: E
authors.initials: E
authors.lastname: Osorio-Cadavid
mesh_terms: D044822:Biodiversity; D003105:Colombia; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S
keywords: MSP-PCR fingerprinting; artificial lake; molecular identification; yeast diversity