title: Role of TGF-β1 in human colorectal cancer and effects after cantharidinate intervention.
abstract: Effects of transforming growth factor-beta (TGF-β) were investigated in human colorectal cancer, and the influence of cantharidinate in inhibiting TGF-β1 expression was explored. Relationships among TGF-β1 and sex, age, tumor size, tumor location, tumor stage were also analyzed. H and E and immunohistochemistry staining were employed to assess colorectal cancer and TGF-β1 expression, respectively. Then, HCT-116 CRC cells were randomly divided into four groups, controls, no serum-treated, chemotherapy and cantharidinate-treated. Immunohistochemistry and real-time PCR were employed to assess the expression of TGF-β1 in CRC cells. Our data showed that the expression of TGF-β1 might be associated with tumor size and tumor location (P<0.05). The expression of TGF-β1 in CRC groups was higher than in adjacent groups (P<0.05). In addition, the expression of TGF-β1 in cantharidinate-treated group was much lower than in CRC group (P<0.05). Taken together, these results suggest that TGF-β1 plays an important role in CRC development. Cantharidinate might inhibit the expression of TGF-β1 and control the development of colorectal cancer.
authors.affiliation: Department of Molecular Medicine, Jilin University, Changchun, China E-mail : gaohc12345@sina.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Hai-Mei
authors.initials: HM
authors.lastname: Gao
authors.forename: Xin
authors.initials: X
authors.lastname: Hua
authors.forename: Ze-Yuan
authors.initials: ZY
authors.lastname: Lu
authors.forename: Hai-Cheng
authors.initials: HC
authors.lastname: Gao
mesh_terms: D002193:Cantharidin / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D045325:HCT116 Cells; D006801:Humans; D009154:Mutation; D011897:Random Allocation; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004791:Enzyme Inhibitors; D016212:Transforming Growth Factor beta; D002193:Cantharidin