title: miR-544a promotes the invasion of lung cancer cells by targeting cadherina 1 in vitro.
abstract: OBJECTIVE
  To find out the effect of miR-544a on the invasion of lung cancer cells and to explore the underlying molecular mechanisms.
  
  
  METHODS
  Micro-ribonucleic acid (miRNA) expression in two different invasive lung cancer cell lines 95C (low invasive ability) and 95D (high invasive ability) was analyzed by miRNA microarray and real-time quantitative polymerase chain reaction (PCR); miR-544a mimic was transfected to 95C, and its invasion ability was detected by transwell migration assay; we predicted the candidate miRNA target genes by TargetScan (Whitehead Institute for Biomedical Research, Cambridge, MA, USA) software and verified the target genes by Western blot.
  
  
  RESULTS
  The expression of miR-544a was significantly increased in 95D in miRNA microarray and quantitative PCR tests (P<0.05). After being transfected with miR-544a mimic, the invasion ability of 95C was enhanced (P<0.01). Moreover, transfection with miR-544a inhibitor decreased the invasion ability of 95D (P<0.01). miR-544a possibly combined with CDH1 (E-cadherin) predicted by the TargetScan analysis. 95C with miR-544a mimic reduced the expression of CDH1 and improved the expression of vimentin, while 95D with miR-544a inhibitor improved the expression of CDH1 and reduced the expression of vimentin.
  
  
  CONCLUSIONS
  miR-544a can promote the invasion of non-small cell lung cancer by downregulation of CDH1 and upregulation of vimentin.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China ; Qingdao Women and Children Hospital, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Mo
authors.affiliation: Qingdao Women and Children Hospital, China.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Qingdao Women and Children Hospital, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liang
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Laboratory Medicine, Qingdao Municipal Hospital, Qingdao, People's Republic of China.
authors.forename: Huahui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, Qingdao Municipal Hospital, Qingdao, People's Republic of China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Xia
keywords: E-cadherin; EMT; NSCLC; microRNA; non-small cell lung cancer