title: Application of ISSR-PCR for rapid strain typing of Debaryomyces hansenii isolated from dry-cured Iberian ham.
abstract: Yeast populations of dry-cured Iberian ham isolated from seven industries in the province of Badajoz were characterized by ISSR-PCR using the (CAG)4 primer and PCR-RFLP of the ITS1-5.8S rRNA-ITS2 fragment, and identified by DNA sequencing. A total of 242 isolates were analyzed, indicating the primary species present was Debaryomyces hansenii at 80.9% of the isolates followed by Candida zeylanoides at 10.3% of the isolates. The remainders of isolates were identified as Yamadazyma triangularis, Sporobolomyces roseus, Meyerozyma guilliermondii, Rhodotorula slooffiae, and Cryptococcus victoriae. The ISSR-PCR method was a fast and reliable method which was able to discriminate species at a level comparable to restriction analyses of the ITS1-5.8S rRNA-ITS2 region. This method allowed for strain typing of D. hansenii, yielding 29 different PCR patterns within 196 isolates. Moreover, ISSR-PCR using the (CAG)4 primer indicated that this technique could be a promising tool for rapid discrimination of yeast starter cultures and spoilage species in dry-cured Iberian ham.
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suarez, 06007 Badajoz, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Gallardo
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suarez, 06007 Badajoz, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Ruiz-Moyano
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suarez, 06007 Badajoz, Spain. Electronic address: ahernandez@unex.es.
authors.forename: A
authors.initials: A
authors.lastname: Hernández
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suarez, 06007 Badajoz, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Benito
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suarez, 06007 Badajoz, Spain.
authors.forename: M G
authors.initials: MG
authors.lastname: Córdoba
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suarez, 06007 Badajoz, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Pérez-Nevado
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suarez, 06007 Badajoz, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martín
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D008461:Meat Products / Q000382:microbiology*; D016533:Mycological Typing Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal
keywords: Dry-cured Iberian ham; ISSR-PCR; Identification; RFLP of ITS-5.8S rRNA; Strain typing; Yeast