title: Effect of ferrocene-substituted porphyrin RL-91 on Candida albicans biofilm formation.
abstract: Ferrocene-substituted porphyrin RL-91 exhibits antifungal activity against opportune human pathogen Candida albicans. RL-91 efficiently inhibits growth of both planktonic C. albicans cells and cells within biofilms without photoactivation. The minimal inhibitory concentration for plankton form (PMIC) was established to be 100 μg/mL and the same concentration killed 80% of sessile cells in the mature biofilm (SMIC80). Furthermore PMIC of RL-91 efficiently prevents C. albicans biofilm formation. RL-91 is cytotoxic for human fibroblasts in vitro in concentration of 10 μg/mL, however it does not cause hemolysis in concentrations of up to 50 μg/mL. These findings open possibility for application of RL-91 as an antifungal agent for external antibiofilm treatment of medical devices as well as a scaffold for further development of porphyrin based systemic antifungals.
authors.affiliation: Friedrich-Alexander University Erlangen-Nuremberg, Department of Chemistry & Pharmacy, Egerlandstr. 1, 91058 Erlangen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Lippert
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11000 Belgrade, Serbia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Vojnovic
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski Trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Mitrovic
authors.affiliation: Friedrich-Alexander University Erlangen-Nuremberg, Dep. Chemistry & Pharmacy and Interdisciplinary Center for Molecular Materials, Henkestr. 42, 91054 Erlangen, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Jux
authors.affiliation: Friedrich-Alexander University Erlangen-Nuremberg, Department of Chemistry & Pharmacy, Egerlandstr. 1, 91058 Erlangen, Germany.
authors.forename: Ivana
authors.initials: I
authors.lastname: Ivanović-Burmazović
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11000 Belgrade, Serbia.
authors.forename: Branka
authors.initials: B
authors.lastname: Vasiljevic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11000 Belgrade, Serbia. Electronic address: nadastankovic@imgge.bg.ac.rs.
authors.forename: Nada
authors.initials: N
authors.lastname: Stankovic
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D005296:Ferrous Compounds / Q000737:chemistry / Q000494:pharmacology*; D000075163:Metallocenes; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D011166:Porphyrins / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000935:Antifungal Agents; D005296:Ferrous Compounds; D000075163:Metallocenes; D011166:Porphyrins; C000595765:RL-91 compound; C004998:ferrocene
keywords: Antifungal; Biofilm; Candida albicans; Ferrocene; Porphyrins