title: Secretory vesicles deliver Cdc42p to sites of polarized growth in S. cerevisiae.
abstract: The activation and localization of the Rho-family GTPase Cdc42p at one pole of a cell is necessary for maintaining an axis of polarized growth in many animal and fungal cells. How the asymmetric distribution of this key regulator of polarized morphogenesis is maintained is not fully understood, though divergent models have emerged from a congruence of multiple studies, including one that posits a role for polarized secretion. Here we show with S. cerevisiae that Cdc42p associates with secretory vesicles in vivo.
authors.affiliation: Department of Biology, University of Virginia, Charlottesville, Virginia, United States of America.
authors.forename: Shubha A
authors.initials: SA
authors.lastname: Dighe
authors.affiliation: Department of Biology, University of Virginia, Charlottesville, Virginia, United States of America; Department of Cell Biology, University of Virginia, Charlottesville, Virginia, United States of America.
authors.forename: Keith G
authors.initials: KG
authors.lastname: Kozminski
mesh_terms: D016764:Cell Polarity; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D015870:Gene Expression; D008854:Microscopy, Electron; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D022142:Secretory Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D010750:Phosphoproteins; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae