title: Reciprocal regulation of adipocyte and osteoblast differentiation of mesenchymal stem cells by Eupatorium japonicum prevents bone loss and adiposity increase in osteoporotic rats.
abstract: Pathological increases in adipogenic potential with decreases in osteogenic differentiation occur in osteoporotic bone marrow cells. Previous studies have shown that bioactive materials isolated from natural products can reciprocally regulate adipogenic and osteogenic fates of bone marrow cells. In this study, we showed that Eupatorium japonicum stem extracts (EJE) suppressed lipid accumulation and inhibited the expression of adipocyte markers in multipotent C3H10T1/2 and primary bone marrow cells. Conversely, EJE stimulated alkaline phosphatase activity and induced the expression of osteoblast markers in C3H10T1/2 and primary bone marrow cells. Daily oral administration of 50 mg/kg of EJE for 6 weeks to ovariectomized rats prevented body weight increase and bone mineral density decrease. Finally, activity-guided fractionation led to the identification of coumaric acid and coumaric acid methyl ester as bioactive anti-adipogenic and pro-osteogenic components in EJE. Taken together, our data indicate a promising possibility of E. japonicum as a functional food and as a therapeutic intervention for preventing osteoporosis and bone fractures.
authors.affiliation: 1 Department of Food Science and Biotechnology, Sungkyunkwan University , Suwon, Korea.
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Kim
authors.forename: Woo-Seok
authors.initials: WS
authors.lastname: Jang
authors.forename: In-Kyoung
authors.initials: IK
authors.lastname: Lee
authors.forename: Jong-Keun
authors.initials: JK
authors.lastname: Kim
authors.forename: Ki-Seung
authors.initials: KS
authors.lastname: Seong
authors.forename: Cho-Rong
authors.initials: CR
authors.lastname: Seo
authors.forename: No-Joon
authors.initials: NJ
authors.lastname: Song
authors.forename: Min-Hyuk
authors.initials: MH
authors.lastname: Bang
authors.forename: Young Min
authors.initials: YM
authors.lastname: Lee
authors.forename: Haeng Ran
authors.initials: HR
authors.lastname: Kim
authors.forename: Ki-Moon
authors.initials: KM
authors.lastname: Park
authors.forename: Kye Won
authors.initials: KW
authors.lastname: Park
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D050156:Adipogenesis / Q000187:drug effects; D050154:Adiposity / Q000187:drug effects; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001851:Bone Diseases, Metabolic / Q000209:etiology / Q000517:prevention & control*; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D003373:Coumaric Acids / Q000032:analysis / Q000494:pharmacology; D036602:Eupatorium / Q000737:chemistry*; D005260:Female; D050356:Lipid Metabolism / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010024:Osteoporosis / Q000150:complications / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003373:Coumaric Acids; D010936:Plant Extracts; C057918:4-coumaric acid methyl ester; D000469:Alkaline Phosphatase
keywords: C3H10T1/2; Eupatorium japonicum; bone marrow; coumaric acid; osteoporosis; ovariectomized rat