title: Dental implant surface treatments may modulate cytokine secretion in Porphyromonas gingivalis-stimulated human gingival fibroblasts: a comparative study.
abstract: Peri-implantitis is an inflammation that affects dental implants and can lead to implant loss. The aim of this study was to analyze the in vitro effect of different implant surface treatments on cytokine production by human gingival fibroblasts (HGFs) stimulated or not with Porphyromonas gingivalis lipopolysaccharide (PgLPS). Six different titanium implants were tested: turned, sandblasted, anodized, acid-etched, TiO2-blasted/acid-etched, and grit-blasted/acid-etched. HGFs were seeded with each implant in a 6-well plate and assayed before LPS treatment (-LPS) or after 36 h of LPS (+LPS) treatment. Protein concentrations were measured using a Pierce bicinchoninic acid (BCA) assay and cytokine secretions were analyzed using a multiplex cytokine array. Scanning electron microscopy was performed for sterile implants and after cell attachment. Protein levels were consistent across all implants indicating that cell growth was uniform (p > 0.05). Sandblasted and turned surfaces significantly increased the secretion of interleukin (IL)-6, -8, -10, MCP-1 and VEGF (p < 0.05) when compared with the other surfaces. PgLPS stimulus increased cytokine secretion in all tested surfaces. In conclusion, different implant surfaces had various effects on HGFs' cytokine secretion. The findings may provide insights into the progression of peri-implantitis.
authors.affiliation: Department of Preventive Dentistry, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Alexander
authors.initials: A
authors.lastname: Stavroullakis
authors.forename: Carlos
authors.initials: C
authors.lastname: Brito
authors.forename: Hong Yang
authors.initials: HY
authors.lastname: Chen
authors.forename: Elena
authors.initials: E
authors.lastname: Bajenova
authors.forename: Anuradha
authors.initials: A
authors.lastname: Prakki
authors.forename: Getulio
authors.initials: G
authors.lastname: Nogueira-Filho
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D015921:Dental Implants; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D005881:Gingiva / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry / Q000633:toxicity*; D016966:Porphyromonas gingivalis / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D016207:Cytokines; D015921:Dental Implants; D008070:Lipopolysaccharides
keywords: Porphyromonas gingivalis; cytokines; fibroblasts; peri-implantitis; titanium