title: The relationship between feed efficiency and the circadian profile of blood plasma analytes measured in beef heifers at different physiological stages.
abstract: The characterization of blood metabolite concentrations over the circadian period and across physiological stages is important for understanding the biological basis of feed efficiency, and may culminate in indirect methods for assessing feed efficiency. Hematological analyses for albumin, urea, creatine kinase, glutamate dehydrogenase, aspartate aminotransferase, carbon dioxide, and acetate were carried out in growing and gestating heifers. These measures were carried out in a sample of 36 Bos taurus crossed beef heifers held under the same husbandry conditions. Hourly blood samples were collected over a 24-h period on three separate sampling occasions, corresponding approximately to the yearling (and open), early-gestation and late-gestation stages. This design was used to determine variation throughout the day, effects due to physiological status and any associations with feed efficiency, as measured by residual feed intake. Blood analyte levels varied with time of day, with the most variation occurring between 0800 and 1600 h. There were also considerable differences in analyte levels across the three physiological stages; for example, creatine kinase was higher (P<0.05) in open heifers, followed by early- and late-gestation heifers. Feed efficiency was also associated with analyte abundance. In more feed-efficient open heifers, there were higher activities of creatine kinase (P<0.05) and aspartate aminotransferase (P<0.05), and lower concentrations of carbon dioxide (P<0.05). Furthermore, in late gestation, more efficient heifers had lower urea concentrations (P<0.05) and lower creatine kinase levels (P<0.05). Over the whole experimental period, carbon dioxide concentrations were numerically lower in more feed efficient heifers (P=0.079). Differences were also observed across physiological stages. For instance, open heifers had increased levels (P<0.05) of creatine kinase, aspartate aminotransferase, carbon dioxide than early and late pregnancy heifers. In essence, this study revealed relevant information about the metabolic profile in the context of feed efficiency and physiological stages. Further optimization of our approach, along with the evaluation of complementary analytes, will aid in the development of robust, indirect assessments of feed efficiency.
authors.affiliation: 1Department of Animal and Poultry Science,University of Guelph,50 Stone Road East - Building 70,Guelph,Ontario,CanadaN1G 2W1.
authors.forename: C V
authors.initials: CV
authors.lastname: Gonano
authors.affiliation: 1Department of Animal and Poultry Science,University of Guelph,50 Stone Road East - Building 70,Guelph,Ontario,CanadaN1G 2W1.
authors.forename: Y R
authors.initials: YR
authors.lastname: Montanholi
authors.affiliation: 1Department of Animal and Poultry Science,University of Guelph,50 Stone Road East - Building 70,Guelph,Ontario,CanadaN1G 2W1.
authors.forename: F S
authors.initials: FS
authors.lastname: Schenkel
authors.affiliation: 1Department of Animal and Poultry Science,University of Guelph,50 Stone Road East - Building 70,Guelph,Ontario,CanadaN1G 2W1.
authors.forename: B A
authors.initials: BA
authors.lastname: Smith
authors.affiliation: 1Department of Animal and Poultry Science,University of Guelph,50 Stone Road East - Building 70,Guelph,Ontario,CanadaN1G 2W1.
authors.forename: J P
authors.initials: JP
authors.lastname: Cant
authors.affiliation: 1Department of Animal and Poultry Science,University of Guelph,50 Stone Road East - Building 70,Guelph,Ontario,CanadaN1G 2W1.
authors.forename: S P
authors.initials: SP
authors.lastname: Miller
mesh_terms: D000085:Acetates / Q000097:blood; D000821:Animal Feed; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002245:Carbon Dioxide / Q000097:blood; D002417:Cattle / Q000502:physiology*; D057906:Circadian Clocks / Q000502:physiology*; D003402:Creatine Kinase / Q000097:blood; D004435:Eating; D005260:Female; D005969:Glutamate Dehydrogenase / Q000097:blood; D008099:Liver / Q000378:metabolism; D011247:Pregnancy; D012709:Serum Albumin / Q000032:analysis; D014508:Urea / Q000097:blood
chemical_list: D000085:Acetates; D012709:Serum Albumin; D002245:Carbon Dioxide; D014508:Urea; D005969:Glutamate Dehydrogenase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase