title: Induction of antibodies and T cell responses by a recombinant influenza virus carrying an HIV-1 TatΔ51-59 protein in mice.
abstract: Recombinant influenza viruses hold promise as vectors for vaccines to prevent transmission of mucosal pathogens. In this study, we generated a recombinant WSN/TatΔ(51-59) virus in which Tat protein lacking residues 51 to 59 of the basic domain was inserted into the N-terminus of the hemagglutinin (HA) of A/WSN/33 virus. The TatΔ(51-59) insertion into the viral HA caused a 2-log reduction in viral titers in cell culture, compared with the parental A/WSN/33 virus, and severely affected virus replication in vivo. Nevertheless, Tat-specific antibodies and T cell responses were elicited upon a single intranasal immunization of BALB/c mice with WSN/TatΔ(51-59) virus. Moreover, Tat-specific immune responses were also detected following vaccine administration via the vaginal route. These data provide further evidence that moderately large HIV antigens can be delivered by chimeric HA constructs and elicit specific immune responses, thus increasing the options for the potential use of recombinant influenza viruses, and their derivatives, for prophylactic and therapeutic vaccines.
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy ; Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Garulli
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Di Mario
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Stillitano
authors.affiliation: National AIDS Center, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Compagnoni
authors.affiliation: National AIDS Center, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Titti
authors.affiliation: National AIDS Center, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cafaro
authors.affiliation: National AIDS Center, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Ensoli
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA ; Division of Virology, Department of Microbiology and Immunology and International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan ; Infection-Induced Host Responses Project, Exploratory Research for Advanced Technology, Saitama 332-0012, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kawaoka
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors.forename: M R
authors.initials: MR
authors.lastname: Castrucci
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics*; D006388:Hemagglutinins / Q000235:genetics; D006801:Humans; D007111:Immunity, Cellular / Q000235:genetics; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology; D007251:Influenza, Human / Q000235:genetics / Q000821:virology; D051379:Mice; D009975:Orthomyxoviridae / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D000914:Antibodies, Viral; D006388:Hemagglutinins; D007252:Influenza Vaccines; D011994:Recombinant Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus