title: Inhibition of constitutive TNF production is associated with PACAP-mediated differentiation in PC12 cells.
abstract: The pituitary adenylate cyclase-activating polypeptide (PACAP) is a trophic neuropeptide that promotes cell survival and neuritogenesis in the central and peripheral nervous system. Our previous transcriptomic studies revealed the down-regulation of the cytokine tumor necrosis factor (TNF) during PACAP-induced PC12 cell differentiation. Here we show that TNF is constitutively expressed in PC12 cells in a manner dependent on NF-κB transcription factor, and that PACAP rapidly inhibits TNF expression and secretion. The inhibition occurs through suppression of RelB subunit of NF-κB, and is likely to prevent the deleterious effects of the cytokine on survival and neurite outgrowth during PC12 cell differentiation.
authors.affiliation: Inserm, U982, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication, Mont-Saint-Aignan, France; Institute for Research and Innovation in Biomedicine, Normandy University, University of Rouen, Mont-Saint-Aignan, France.
authors.forename: Destiny-Love
authors.initials: DL
authors.lastname: Manecka
authors.affiliation: CNRS, UPR 3212, INCI, Strasbourg, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Lelièvre
authors.affiliation: Inserm, U982, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication, Mont-Saint-Aignan, France; Institute for Research and Innovation in Biomedicine, Normandy University, University of Rouen, Mont-Saint-Aignan, France. Electronic address: youssef.anouar@univ-rouen.fr.
authors.forename: Youssef
authors.initials: Y
authors.lastname: Anouar
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D005786:Gene Expression Regulation; D006801:Humans; D016501:Neurites / Q000378:metabolism; D016716:PC12 Cells; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha
keywords: Apoptosis; Neurite outgrowth; PC12 cells; Pituitary adenylate cyclase-activating polypeptide; Tumor necrosis factor