title: Wearable multi-channel microelectrode membranes for elucidating electrophysiological phenotypes of injured myocardium.
abstract: Understanding the regenerative capacity of small vertebrate models has provided new insights into the plasticity of injured myocardium. Here, we demonstrate the application of flexible microelectrode arrays (MEAs) in elucidating electrophysiological phenotypes of zebrafish and neonatal mouse models of heart regeneration. The 4-electrode MEA membranes were designed to detect electrical signals in the aquatic environment. They were micro-fabricated to adhere to the non-planar body surface of zebrafish and neonatal mice. The acquired signals were processed to display an electrocardiogram (ECG) with high signal-to-noise-ratios, and were validated via the use of conventional micro-needle electrodes. The 4-channel MEA provided signal stability and spatial resolution, revealing the site-specific electrical injury currents such as ST-depression in response to ventricular cryo-injury. Thus, our polymer-based and wearable MEA membranes provided electrophysiological insights into long-term conduction phenotypes for small vertebral models of heart injury and regeneration with a translational implication for monitoring cardiac patients.
authors.affiliation: Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USA.
authors.forename: Hung
authors.initials: H
authors.lastname: Cao
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Zhang
authors.forename: Joyce
authors.initials: J
authors.lastname: Tai
authors.forename: Juhyun
authors.initials: J
authors.lastname: Lee
authors.forename: Ali
authors.initials: A
authors.lastname: Darehzereshki
authors.forename: Malcolm
authors.initials: M
authors.lastname: Bersohn
authors.forename: Ching-Ling
authors.initials: CL
authors.lastname: Lien
authors.forename: Neil C
authors.initials: NC
authors.lastname: Chi
authors.forename: Yu-Chong
authors.initials: YC
authors.lastname: Tai
authors.forename: Tzung K
authors.initials: TK
authors.lastname: Hsiai
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology; D004562:Electrocardiography; D004569:Electroencephalography; D055724:Electrophysiological Phenomena; D004594:Electrophysiology / Q000295:instrumentation*; D004867:Equipment Design; D006321:Heart / Q000502:physiology; D006329:Heart Conduction System / Q000502:physiology; D006335:Heart Injuries / Q000175:diagnosis / Q000473:pathology*; D051379:Mice; D008839:Microelectrodes; D018670:Monitoring, Ambulatory / Q000379:methods*; D009206:Myocardium / Q000473:pathology*; D010641:Phenotype; D011108:Polymers / Q000737:chemistry; D012038:Regeneration; D012640:Seizures / Q000473:pathology / Q000503:physiopathology; D059629:Signal-To-Noise Ratio; D015027:Zebrafish
chemical_list: D011108:Polymers