title: In vitro anti-proliferative effects on NB4 human leukemia cells and physicochemical screening of Pleurotus sp. (higher Basidiomycetes) mycelia from Cuba.
abstract: This study examined the phytochemical profile and the in vitro anti-proliferative effects of a hot water mycelial extract from the edible mushroom Pleurotus sp. on NB4 human leukemia cells. Flow-cytometry analyses were used to measure cell viability, cell cycle, and apoptosis in cells incubated 24 h with the extract at doses of 100 and 200 µg/mL. Pleurotus sp. extract reduced cell viability, particularly at the concentration of 200 µg/mL to 82% compared to control cells, and induced apoptosis demonstrated by an increase in the number of annexin V-FITC+ cells (25% at 200 µg/mL). The NB4 cells were arrested in the G2/M phase thus supporting a cell-cycle dependent anticancer mechanism. Although carbohydrates (76.8%, w/w) appear to be the most important antitumor compound, secondary metabolites-like phenolics would also contribute to the anti-proliferative activity. The results indicate that Pleurotus sp. mycelia obtained by submerged fermentation may be an interesting renewable resource for developing functional foods and new antitumor therapeutic agents.
authors.affiliation: Centre of Studies for Industrial Biotechnology (CEBI), Universidad de Oriente, Reparto Jimenez, Santiago de Cuba 5. CP 90 500, Cuba.
authors.forename: Humberto J
authors.initials: HJ
authors.lastname: Morris
authors.affiliation: Department of Biology, Faculty of Natural Sciences, Universidad de Oriente, Santiago de Cuba 5. CP 90 500, Cuba.
authors.forename: Edgar
authors.initials: E
authors.lastname: Hernández
authors.affiliation: Centre of Studies for Industrial Biotechnology (CEBI), Universidad de Oriente, Reparto Jimenez, Santiago de Cuba 5. CP 90 500, Cuba.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Llauradó
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain.
authors.forename: María Cristina
authors.initials: MC
authors.lastname: Tejedor
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Sancho
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Herraez
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Boyano-Adánez
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain.
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: García-Pérez
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Diez
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003462:Cuba; D006131:Growth Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000503:physiopathology*; D025282:Mycelium / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020076:Pleurotus / Q000737:chemistry*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D006131:Growth Inhibitors; D010936:Plant Extracts