title: [Diammonium glycyrrhizinate promotes aquaporin-5 in LPS-induced acute lung injury via inactivation of NF-kappaB].
abstract: OBJECTIVE
  To determine the therapeutic value and associated mechanism of diammonium glycyrrhizinate (DG) on the expression of AQP-5 in lipapolysacchairides (LPS)-induced acute lung injury in mice.
  
  
  METHODS
  Thirty male BALB/c mice were randomly divided into three groups equally: Control, LPS+DG and LPS. HE staining and lung injury score system were used to evaluate the pathological changes in the lung tissues. Wet to dry ratio (W/D) was used to measure the degree of lung edema. RT-PCR and Western blot were obtained to measure AQP-5 expression. Total NF-kappaB p65 and phospho-NF-kappaB p65 (p-NF-kappaB p65) were evaluated by Western blot.
  
  
  RESULTS
  After 3 days of LPS intratracheal injection, severe pathological changes, increased W/D, down-regulated AQP-5 expression and increased p-NF-kappaB p65/total NF-kappaB p65 were observed. Compared with mice in the LPS group, mice in the LPS+DG group had more significantly ameliorated pathological changes and increased W/ D, up-regulated AQP-5 expression, and reduced p-NF-kappaB p65/total NF-kappaB p65.
  
  
  CONCLUSIONS
  DG up-regulates AQP-5 in vivo, possibly resulting from inactivation of NF-kappaB.
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.forename: Tao
authors.initials: T
authors.lastname: Zhu
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Fu-qiang
authors.initials: FQ
authors.lastname: Wen
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism*; D000818:Animals; D051402:Aquaporin 5 / Q000378:metabolism*; D015536:Down-Regulation; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011654:Pulmonary Edema / Q000473:pathology; D051996:Transcription Factor RelA / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C496038:Aqp5 protein, mouse; D051402:Aquaporin 5; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; D019695:Glycyrrhizic Acid