title: Extended characterization of Corynebacterium pyruviciproducens based on clinical strains from Canada and Switzerland.
abstract: The species Corynebacterium pyruviciproducens was described in 2010 based on the features of a single strain. In this report, we describe the chemotaxonomic and phenotypic characteristics of 11 C. pyruviciproducens clinical strains isolated in Switzerland and Canada in comparison to the strain 06-17730(T). Heterogeneities within the type strain were found in the 16S rRNA gene and in biochemical markers. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification of this species could not be achieved since currently this bacterial species is not included in the corresponding database. Reliable identification is obtained only with sequence-based identification tools. Results of antimicrobial susceptibility testing of this species with an extended panel of antimicrobials are presented here for the first time.
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland daniel.goldenberger@usb.ch.
authors.forename: D
authors.initials: D
authors.lastname: Goldenberger
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors.forename: V
authors.initials: V
authors.lastname: Hinić
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Turan
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Schultheiss
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: A L
authors.initials: AL
authors.lastname: Pacheco
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Frei
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada University of Manitoba, Department of Medical Microbiology, Winnipeg, Manitoba, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Bernard
mesh_terms: D015373:Bacterial Typing Techniques; D002170:Canada; D016000:Cluster Analysis; D003352:Corynebacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D003354:Corynebacterium Infections / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013557:Switzerland
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S