title: Evaluation of CD52 positive sperms in subfertile human semen samples: Is there any relationship with main semen parameters?
abstract: BACKGROUND
  Sperm maturation and sperm membrane integration are the most important elements in male fertility. CD52 is one of the antigens. CD52 is a GPI (glycosylphosphatidylinositol) anchored that express on lymphocytes and epididymal cells. This antigen bind to sperm membrane during transition sperm from epididymal duct as well as its relationship with semenogelins in human seminal plasma. The aim of this study was to obtain any association between the percentage of CD52 positive sperms with main semen parameters such as percentage of motile sperms, percentage of sperm with normal morphology, and the presence of normal viscosity.
  
  
  METHODS
  Semen samples from subfertile men were analyzed, the samples totally were 45 that divided according to their motility into three groups, first one, more than 40%, second one 10-40%, and the third one under 10% total motility. Fifteen samples in each group were evaluated by semen analysis according to WHO 2010 guidelines for infertility laboratory. Sperms were washed by Ham's F-10 and immunostaining with the monoclonal antibody CAMPATH-1G and then analyzed by flow cytometry. We compared each of the groups based on their motility and the data were analyzed by SPSS 20.
  
  
  RESULTS
  Correlation between CD52 labeling and sperm motility was negatively significant, in the second group (r = -0.592, P = 0.020) and in the third group (r = -0.805, P = 0.00).
  
  
  CONCLUSIONS
  Our results showed that the correlation between CD52 labeling and sperm motility was negatively significant, but we did not observe any relation with other semen parameters, such as sperm normal morphology, sperm concentration, and semen viscosity.
authors.affiliation: Head of Infertility Laboratory, Shahid Beheshti Hospital, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Roshanak
authors.initials: R
authors.lastname: Aboutorabi
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mazani
authors.affiliation: Department of Physiology, School of Medicine, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Laleh
authors.initials: L
authors.lastname: Rafiee
keywords: CD52; infertility; sperm parameters