title: Characterisation and bioactivity of oosporein produced by endophytic fungus Cochliobolus kusanoi isolated from Nerium oleander L.
abstract: Bioactive compounds comprising secondary metabolites produced by endophytic fungi have wide applications in pharmacology and agriculture. Isolation, characterisation and evaluation of biological activities of secondary metabolites were carried out from Cochliobolus kusanoi an endophytic fungus of Nerium oleander L. The fungus was identified based on 18S rDNA sequence analysis. There are no reports available on the compounds of C.kusanoi hence, antimicrobial metabolite produced by this fungus was extracted and purified by fractionation using hexane, diethyl ether, dichloromethane, ethyl acetate and methanol. Out of all the solvent fractions, the methanol fraction exhibited better antimicrobial activity which was further purified and characterised as oosporein. Oosporein from C.kusanoi exhibited broad spectrum in vitro antimicrobial, antioxidant and cytotoxic activities. The characterisation and antioxidant activity of oosporein from C. kusanoi are reported for the first time.
authors.affiliation: a Department of Microbiology and Biotechnology , Bangalore University , Jnanabharathi Campus, Bangalore , Karnataka 560 056 , India.
authors.forename: Ramesha
authors.initials: R
authors.lastname: Alurappa
authors.forename: Madhusudhan Reddy Muthukurpalya
authors.initials: MR
authors.lastname: Bojegowda
authors.forename: Vijith
authors.initials: V
authors.lastname: Kumar
authors.forename: Naveen Kumar
authors.initials: NK
authors.lastname: Mallesh
authors.forename: Srinivas
authors.initials: S
authors.lastname: Chowdappa
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry*; D016227:Benzoquinones / Q000737:chemistry / Q000494:pharmacology*; D006224:Cricetinae; D004354:Drug Screening Assays, Antitumor; D005658:Fungi / Q000187:drug effects; D006801:Humans; D030007:Nerium / Q000382:microbiology*
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D016227:Benzoquinones; C008706:oosporein
keywords: Cochliobolus kusanoi; Nerium oleander L; anticancer activity; antimicrobial activity; antioxidant activity; oosporein