title: Expression and enzymatic activity of phenylalanine ammonia-lyase and p-coumarate 3-hydroxylase in mango (Mangifera indica 'Ataulfo') during ripening.
abstract: Phenylalanine ammonia lyase (PAL) and p-coumarate 3-hydroxylase (C3H) are key enzymes in the phenylpropanoid pathway. The relative expression of PAL and C3H was evaluated in mango fruit cultivar 'Ataulfo' in four ripening stages (RS1, RS2, RS3, and RS4) by quantitative polymerase chain reaction. In addition, enzyme activity of PAL and C3H was determined in mango fruits during ripening. The PAL levels were downregulated at the RS2 and RS3 stages, while C3H levels were upregulated in fruits only at RS3. The enzyme activity of PAL followed a pattern that was different from that of the PAL expression, thus suggesting regulation at several levels. For C3H, a regulation at the transcriptional level is suggested because a similar pattern was revealed by its activity and transcript level. In this study, the complexity of secondary metabolite biosynthesis regulation is emphasized because PAL and C3H enzymes are involved in the biosynthesis of several secondary metabolites that are active during all mango ripening stages.
authors.affiliation: Center of Research in Food & Development CIAD, Hermosillo, Sonora, Mexico.
authors.forename: H
authors.initials: H
authors.lastname: Palafox-Carlos
authors.affiliation: Center of Research in Food & Development CIAD, Hermosillo, Sonora, Mexico.
authors.forename: C A
authors.initials: CA
authors.lastname: Contreras-Vergara
authors.affiliation: Center of Research in Food & Development CIAD, Hermosillo, Sonora, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Muhlia-Almazán
authors.affiliation: Center of Research in Food & Development CIAD, Hermosillo, Sonora, Mexico.
authors.forename: M A
authors.initials: MA
authors.lastname: Islas-Osuna
authors.affiliation: Center of Research in Food & Development CIAD, Hermosillo, Sonora, Mexico gustavo@ciad.mx.
authors.forename: G A
authors.initials: GA
authors.lastname: González-Aguilar
mesh_terms: D003001:Cloning, Molecular; D005638:Fruit / Q000235:genetics / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant; D031022:Mangifera / Q000235:genetics* / Q000254:growth & development; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D010650:Phenylalanine Ammonia-Lyase / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D006899:Mixed Function Oxygenases; D010650:Phenylalanine Ammonia-Lyase