title: In silico study of potential autoimmune threats from rotavirus infection.
abstract: Rotavirus, the major cause of infantile nonbacterial diarrhea, was found to be associated with development of diabetes-associated auto-antibodies. In our study we tried to find out further potential autoimmune threats of this virus using bioinformatics approach. We took rotaviral proteins to study similarity with Homo sapiens proteome and found most conserved structural protein VP6 matches at two regions with ryanodine receptor, an autoimmune target associated with myasthenia gravis. Myasthenia gravis, a chronic neurodegenerative autoimmune disorder with no typical known reason, is characterized by fluctuating muscle weakness which is typically enhanced during muscular effort. Affected patients generate auto antibodies against mainly acetyl choline receptor and sarcoplasmic reticulum calcium-release channel protein ryanodine receptor. Further, we observed that two regions which matched with ryanodine receptor remain conserved in all circulating rotaviral strains and showed significant antigenecity with respect to myasthenia gravis associated HLA haplotypes. Overall, our study detected rotaviral VP6 as a potential threat for myasthenia gravis and enlighten an area of virus associated autoimmune research.
authors.affiliation: Department of Physics, Jadavpur University, Kolkata 700 032, India. Electronic address: tapati.sarkar@yahoo.com.
authors.forename: Tapati
authors.initials: T
authors.lastname: Sarkar
authors.affiliation: Department of Physics, Jadavpur University, Kolkata 700 032, India.
authors.forename: Sukhen
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Physics, Jadavpur University, Kolkata 700 032, India.
authors.forename: Papiya
authors.initials: P
authors.lastname: Nandy
authors.affiliation: Department of Virology, National Institute of Cholera and Enteric Diseases, Kolkata 700010, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Bhowmick
authors.affiliation: Centre for Interdisciplinary Research and Education, Kolkata 700032, India.
authors.forename: Ashesh
authors.initials: A
authors.lastname: Nandy
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry* / Q000276:immunology; D015551:Autoimmunity; D001665:Binding Sites; D002213:Capsid / Q000737:chemistry / Q000276:immunology; D036022:Capsid Proteins / Q000737:chemistry* / Q000276:immunology; D019295:Computational Biology; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D006680:HLA Antigens / Q000737:chemistry* / Q000276:immunology; D006239:Haplotypes; D006801:Humans; D008958:Models, Molecular; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D009157:Myasthenia Gravis / Q000150:complications / Q000276:immunology / Q000821:virology; D011485:Protein Binding; D012401:Rotavirus / Q000737:chemistry* / Q000276:immunology; D012400:Rotavirus Infections / Q000150:complications / Q000276:immunology / Q000821:virology; D019837:Ryanodine Receptor Calcium Release Channel / Q000737:chemistry* / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; D006680:HLA Antigens; D019837:Ryanodine Receptor Calcium Release Channel; C061332:VP6 protein, Rotavirus
keywords: Autoimmunity; Molecular mimicry; Myasthenia gravis; Rota virus