title: Effect of honey consumption on intestinal motility in male albino rats.
abstract: This study investigated the effects of honey on intestinal motility and transit using twenty (20) male albino rats of Wistar strain weighing 210-220g. The rats were randomly grouped into control and honey-fed (test) groups of ten (10) rats each. The control group was fed on normal rat chow ( Pfizer Company, Nigeria ) and water while the test group was fed on rat feed, water and honey ( 1 ml of honey to every 10 ml initial drinking water daily) for twenty two (22) weeks after which the rats were starved over night before the experiment and sacrificed by stunning. Laparatomy was immediately performed, proximal and distal portions of the intestine identified, cut and put in aerated tyrode solution. Cut sections of the ileum (2-3cm) were mounted on organ bath instrument for motility experiment with varying concentrations of acetylcholine and carbachol. Contractions were recorded as well as the intestinal transit in each group and lengths of intestine with total mean values calculated. Results obtained showed that honey significantly decreased (p&lt;0.01 ) intestinal transit in the test group (21.15±0.75 ) compared with the control group ( 35.96±1.15); decreased intestinal motility in the test group compared with the control and caused significant percentage reduction of intestinal motility with varied concentrations of acetylcholine and carbachol in the test group ( Ach-75.00±0.75%; Carbachol-79.00±0.28%) compared with the control group (Ach-62.00±0.39%; Carbachol-51.00±0.39%). In conclusion, unprocessed Nigerian honey decreased intestinal transit, caused intestinal smooth muscle inhibition and motility and reduced sensitivity of gastrointestinal tract to cholinergic agents.
authors.affiliation: Department of Physiology, College of Medicine and Health Sciences, Imo State University, Owerri. chinayo58@yahoo.ca.
authors.forename: E A
authors.initials: EA
authors.lastname: Alagwu
authors.forename: J N
authors.initials: JN
authors.lastname: Egwurugwu
authors.forename: R O
authors.initials: RO
authors.lastname: Nneli
authors.forename: F
authors.initials: F
authors.lastname: Oguike
authors.forename: E E
authors.initials: EE
authors.lastname: Osim
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D018679:Cholinergic Agonists / Q000494:pharmacology; D004032:Diet; D004305:Dose-Response Relationship, Drug; D018565:Food-Drug Interactions; D005769:Gastrointestinal Motility / Q000187:drug effects; D005772:Gastrointestinal Transit; D006722:Honey; D007082:Ileum / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D017208:Rats, Wistar
chemical_list: D018679:Cholinergic Agonists; D002217:Carbachol; D000109:Acetylcholine