title: Asymmetrical flow field-flow fractionation with multi-angle light scattering and quasi-elastic light scattering for characterization of polymersomes: comparison with classical techniques.
abstract: Polymersomes formed from amphiphilic block copolymers, such as poly(ethyleneoxide-b-ε-caprolactone) (PEO-b-PCL) or poly(ethyleneoxide-b-methylmethacrylate), were characterized by asymmetrical flow field-flow fractionation coupled with quasi-elastic light scattering (QELS), multi-angle light scattering (MALS), and refractive index detection, leading to the determination of their size, shape, and molecular weight. The method was cross-examined with more classical ones, like batch dynamic and static light scattering, electron microscopy, and atomic force microscopy. The results show good complementarities between all the techniques; asymmetrical flow field-flow fractionation being the most pertinent one when the sample exhibits several different types of population.
authors.affiliation: Université de Toulouse, UPS/CNRS, IMRCP, 118 route de Narbonne, 31062, Toulouse Cedex 9, France.
authors.forename: Ugo
authors.initials: U
authors.lastname: Till
authors.forename: Mireille
authors.initials: M
authors.lastname: Gaucher-Delmas
authors.forename: Pascale
authors.initials: P
authors.lastname: Saint-Aguet
authors.forename: Glenn
authors.initials: G
authors.lastname: Hamon
authors.forename: Jean-Daniel
authors.initials: JD
authors.lastname: Marty
authors.forename: Christophe
authors.initials: C
authors.lastname: Chassenieux
authors.forename: Bruno
authors.initials: B
authors.lastname: Payré
authors.forename: Dominique
authors.initials: D
authors.lastname: Goudounèche
authors.forename: Anne-Françoise
authors.initials: AF
authors.lastname: Mingotaud
authors.forename: Frédéric
authors.initials: F
authors.lastname: Violleau
mesh_terms: D004867:Equipment Design; D031521:Fractionation, Field Flow / Q000295:instrumentation*; D008027:Light; D020366:Methylmethacrylate / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D012542:Scattering, Radiation; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D011091:Polyesters; D013501:Surface-Active Agents; C458722:polyethylene oxide-polycaprolactone copolymer; D020366:Methylmethacrylate