title: Behavioral effect of dipeptide NGF mimetic GK-2 in an in vivo model of rat traumatic brain injury and its neuroprotective and regenerative properties in vitro.
abstract: A protective behavioral effect of a nerve growth factor dipeptide mimetic GK-2 in the model of open focal trauma of rat brain sensorimotor cortex and its antioxidative and regenerative properties in cultures of rat cerebellar granule cells and mouse embryonal spinal ganglion, respectively, were studied. Intraperitoneal injections of GK-2 (1 mg/kg) for 5 days daily after traumatic brain injury improved significantly motor function of limbs. Moreover, supplementation the incubation medium with GK-2 (0.5-1.5 mg/l) decreased neuronal death induced by H2O2 in cerebellar granule cell cultures and stimulated neurite outgrowth from cultured mouse embryonal spinal ganglia. Our results suggest that GK-2 exhibits pronounced positive behavioral effect in vivo as well as neuroprotective and regenerative effects in vitro, and that these neuroprotective properties probably associated with cell survival but not with cell differentiation pathway.
authors.affiliation: 1Research Center of Neurology, Russian Academy of Medical Sciences , Volokolamskoe Shosse 80, Moscow , Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Stelmashook
authors.forename: Elisaveta E
authors.initials: EE
authors.lastname: Genrikhs
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Novikova
authors.forename: Igor V
authors.initials: IV
authors.lastname: Barskov
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Gudasheva
authors.forename: Sergey B
authors.initials: SB
authors.lastname: Seredenin
authors.forename: Leonid G
authors.initials: LG
authors.lastname: Khaspekov
authors.forename: Nickolay K
authors.initials: NK
authors.lastname: Isaev
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001930:Brain Injuries / Q000150:complications / Q000188:drug therapy*; D002452:Cell Count; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D004151:Dipeptides / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005727:Ganglia, Spinal / Q000187:drug effects; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects*; D020932:Nerve Growth Factor / Q000494:pharmacology; D009422:Nervous System Diseases / Q000188:drug therapy* / Q000209:etiology; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004151:Dipeptides; D018696:Neuroprotective Agents; C572946:bis(N-succinyl-glutamyllysin)hexamethylenediamine; D020932:Nerve Growth Factor
keywords: GK-2; cerebellar granule cultures; dipeptide mimetic of nerve growth factor; neuroprotection; traumatic brain injury