title: DNA-length-dependent fluorescent sensing based on energy transfer in self-assembled multilayers.
abstract: In this paper, a novel DNA-length-dependent fluorescent sensor was constructed based on the fluorescence resonance energy transfer. In the self-assembled multilayers (Quartz/GO/PDDA/Tx-DNA/PDDA/ZnO@CdS), ZnO@CdS and graphene oxide(GO) were employed as an energy donor and an energy acceptor, respectively. Single-stranded Tx-DNA (x represents different chain length of DNA) and poly(diallydimethylammonium) chloride (PDDA) were used as a linker. In the presence of complementary Px-DNA, the formation of double-stranded DNA leads to a change in chain length and achieves the purpose of changing the distance between ZnO@CdS and GO. Thereby, it enhances the efficiency of energy transfer between ZnO@CdS and GO resulting in the quench of fluorescence of ZnO@CdS, and thus different length DNA sequence was detected.
authors.affiliation: College of Materials Science and Engineering, Huaqiao University, Xiamen 361021, China. Electronic address: sunxy@hqu.edu.cn.
authors.forename: Xiang-Ying
authors.initials: XY
authors.lastname: Sun
authors.affiliation: College of Materials Science and Engineering, Huaqiao University, Xiamen 361021, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: College of Materials Science and Engineering, Huaqiao University, Xiamen 361021, China.
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Sun
authors.affiliation: College of Materials Science and Engineering, Huaqiao University, Xiamen 361021, China.
authors.forename: Yaming
authors.initials: Y
authors.lastname: Yu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis*; D004867:Equipment Design; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation*; D006108:Graphite / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D010087:Oxides; D006108:Graphite; D004247:DNA
keywords: DNA; Fluorescence resonance energy transfer; Graphene oxide; Quantum dots; Self-assembled multilayers