title: Determination of zofenopril and its active metabolite in human plasma using high-performance liquid chromatography combined with a triple-quadruple tandem mass spectrometer.
abstract: A simple, selective and sensitive LC-MS-MS method has been developed and validated to simultaneously quantify zofenopril and its active metabolite zofenoprilat in human plasma, using diazepam as internal standard. 1,4-Dithiothreitol was used as a reducer to release and stabilize the thiol group of zofenoprilat from dimer and mixed forms with endogenous thiols in the treatment of plasma samples. After a liquid-liquid extraction with methyl tert-butyl ether under acidic conditions, the post-treatment samples were analyzed on an Agilent ZORBAX Eclipse XDB-C8 column interfaced with a triple-quadruple tandem mass spectrometer using positive electrospray ionization. A solution of methanol and 0.1% formic acid solution (85 : 15, v/v) was used as the isocratic mobile phase with a flow rate of 0.2 mL/min. The method was validated to demonstrate the specificity, lower limit of quantitation, accuracy and precision of measurements. The validated LC-MS-MS method has been successfully applied to study the pharmacokinetics of zofenopril calcium in healthy Chinese volunteers.
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, Center for Instrumental Analysis, China Pharmaceutical University, Ministry of Education, 24 Tongjia Lane, Nanjing, Jiangsu 210009, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, Center for Instrumental Analysis, China Pharmaceutical University, Ministry of Education, 24 Tongjia Lane, Nanjing, Jiangsu 210009, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, Center for Instrumental Analysis, China Pharmaceutical University, Ministry of Education, 24 Tongjia Lane, Nanjing, Jiangsu 210009, P. R. China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Luo
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, Center for Instrumental Analysis, China Pharmaceutical University, Ministry of Education, 24 Tongjia Lane, Nanjing, Jiangsu 210009, P. R. China State Key Laboratory of Natural Medicines China Pharmaceutical University, Nanjing, Jiangsu 210009, P. R. China zunjianzhangcpu@hotmail.com.
authors.forename: Zunjian
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Dermatology, Chinese Academy of Medical Sciences, Perking Union Medical Sciences, Perking Union Medical College, Nanjing 210042, P. R. China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Ma
mesh_terms: D000328:Adult; D002216:Captopril / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
chemical_list: C044958:zofenopril; D002216:Captopril