title: The changeable nervous system: studies on neuroplasticity in cerebellar cultures.
abstract: Circuit reorganization after injury was studied in a cerebellar culture model. When cerebellar cultures derived from newborn mice were exposed at explantation to a preparation of cytosine arabinoside that destroyed granule cells and oligodendrocytes and compromised astrocytes, Purkinje cells surviving in greater than usual numbers were unensheathed by astrocytic processes and received twice the control number of inhibitory axosomatic synapses. Purkinje cell axon collaterals sprouted and many of their terminals formed heterotypical synapses with other Purkinje cell dendritic spines. The resulting circuit reorganization preserved inhibition in the cerebellar cortex. Following this reorganization, replacement of the missing granule cells and glia was followed by a restitution of the normal circuitry. Most of these developmental and reconstructive changes were not dependent on neuronal activity, the major exception being inhibitory synaptogenesis. The full complement of inhibitory synapses did not develop in the absence of neuronal activity, which could be mitigated by application of exogenous TrkB receptor ligands. Inhibitory synaptogenesis could also be promoted by activity-induced release of endogenous TrkB receptor ligands or by antibody activation of the TrkB receptor.
authors.affiliation: Department of Neurology, Oregon Health & Science University, Portland, OR, USA. Electronic address: seilf@comcast.net.
authors.forename: Fredrick J
authors.initials: FJ
authors.lastname: Seil
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000502:physiology*; D006801:Humans; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000502:physiology*
keywords: Activity-dependent plasticity; Astrocytes; Cerebellar cultures; Circuit reorganization; Collateral sprouting; Heterotypical synapses; Inhibitory synaptogenesis; Purkinje cells; TrkB receptor