title: Expressing osteogenic growth peptide in the rabbit bone mesenchymal stem cells increased alkaline phosphatase activity and enhanced the collagen accumulation.
abstract: OBJECTIVE
  The multipotent mesenchymal stem cells (MSCs) were distributed in the bone marrow stroma, and could generate all of the different skeletal cell lineages. The osteogenic differentiation of MSCs, which is indicated by the increased alkaline phosphatase (ALP) activity and the enhanced accumulation of collagen, could be induced by a tetradecapeptide termed osteogenic growth peptide (OGP). It has been hypothesized that the OGP induces the osteogenic differentiation of MSCs probably through regulating the fibroblast growth factor signaling pathways. Although the chemically synthesized OGP was widely applied to study the osteogenic differentiation of MSCs, transferring and expressing OGP gene in target cells is more desirable, especially for gene therapy, given the advantages and convenience on the stable expression of OGP.
  
  
  METHODS
  In this study, we attempt to test the effect of OGP gene transfection; we constructed a eukaryotic expression vector, pcDNA3.1-OGP, which contained the OGP-coding DNA fragment. Subsequently, the vector was transfected into the rabbit MSCs.
  
  
  RESULTS
  A significant increase of ALP activity was detected in the supernatant of pcDNA3.1-OGP transfected MSCs, and the enhanced collagen accumulation, which was inferred by the increased hydroxyproline content and RT-PCR.
  
  
  CONCLUSIONS
  These results implied that transfecting the OGP-expressing vectors into MSCs might induce the osteogenic differentiation of MSCs.
authors.affiliation: The Second Hospital of Harbin Medical University, Harbin, China. songcenlv699@163.com.
authors.forename: G
authors.initials: G
authors.lastname: An
authors.forename: Z
authors.initials: Z
authors.lastname: Xue
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.forename: Q-K
authors.initials: QK
authors.lastname: Deng
authors.forename: Y-S
authors.initials: YS
authors.lastname: Wang
authors.forename: S-C
authors.initials: SC
authors.lastname: Lv
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism*; D006657:Histones / Q000096:biosynthesis* / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000201:enzymology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits; D014162:Transfection
chemical_list: D000199:Actins; D024042:Collagen Type I; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C074533:osteogenic growth peptide; D000469:Alkaline Phosphatase