title: Polypyrrole-coated electrospun poly(lactic acid) fibrous scaffold: effects of coating on electrical conductivity and neural cell growth.
abstract: Neuronal activities play critical roles in both neurogenesis and neural regeneration. In that sense, electrically conductive and biocompatible biomaterial scaffolds can be applied in various applications of neural tissue engineering. In this study, we fabricated a novel biomaterial for neural tissue engineering applications by coating electrospun poly(lactic acid) (PLA) nanofibers with a conducting polymer, polypyrole (PPy), via admicellar polymerization. Optimal conditions for polymerization and preparation of PPy-coated electrospun PLA nanofibers were obtained by comparing results from scanning electron microscopy, X-ray photoelectron spectrometer, and surface conductivity tests. In vitro cell culture experiments showed that PPy-coated electrospun PLA fibrous scaffold is not toxic. The scaffold could support attachment and migration of neural progenitor cells. Neurons derived from progenitor exhibited long neurite outgrowth under electrical stimulation. Our study concluded that PPy-coated electrospun PLA fibers had a good biocompatibility with neural progenitor cells and may serve as a promising material for controlling progenitor cell behaviors and enhancing neural repair.
authors.affiliation: a Center of Excellence on Petrochemical and Materials Technology (PETROMAT) , Soi Chula 12, Phyathai Rd., Pathumwan, Bangkok 10330 , Thailand.
authors.forename: Thitima
authors.initials: T
authors.lastname: Sudwilai
authors.forename: Jun Jye
authors.initials: JJ
authors.lastname: Ng
authors.forename: Chatikorn
authors.initials: C
authors.lastname: Boonkrai
authors.forename: Nipan
authors.initials: N
authors.lastname: Israsena
authors.forename: Surawut
authors.initials: S
authors.lastname: Chuangchote
authors.forename: Pitt
authors.initials: P
authors.lastname: Supaphol
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004553:Electric Conductivity; D006624:Hippocampus / Q000166:cytology; D019344:Lactic Acid / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects; D009474:Neurons / Q000166:cytology* / Q000187:drug effects*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D019344:Lactic Acid; C033616:poly(lactide)
keywords: electrospinning; nerve cell; poly(lactic acid); polypyrole; scaffold