title: Dynamics of cocaine- and amphetamine-regulated transcript containing cell changes in the adrenal glands of two kidney, one clip rats.
abstract: Taking into consideration the homeostatic disorders resulting from renal hypertension and the essential role of cocaine- and amphetamine-regulated transcript (CART) in maintaining homeostasis by regulating many functions of the body, the question arises as to what extent the renovascular hypertension affects the morphology and dynamics of changes of CART-containing cells in the adrenal glands. The aim of the present study was to examine the distribution, morphology, and dynamics of changes of CART-containing cells in the adrenal glands of "two kidney, one clip" (2K1C) renovascular hypertension model in rats. The studies were carried out on the adrenal glands of rats after 3, 14, 28, 42, and 91 days from the renal artery clipping procedure. To identify neuroendocrine cells, immunohistochemical reaction was performed with the use of a specific antibody against CART. It was revealed that renovascular hypertension causes changes in the endocrine cells containing CART in the adrenal glands of rats. The changes observed in the endocrine cells depend on the time when the rats with experimentally induced hypertension were examined. In the first period of hypertension, the number and immunoreactivity of CART-containing cells were decreased, while from the 28-day test, it significantly increased, as compared to the control rats. CART is relevant to the regulation of homeostasis in the cardiovascular system and seems to be involved in renovascular hypertension. The results of the present work open the possibility of new therapeutic perspectives for the treatment of arterial hypertension, since CART function is involved in their pathophysiology.
authors.affiliation: Department of Histology and Cytophysiology, Medical University, 15-222 Bialystok, Poland kasacka@umb.edu.pl.
authors.forename: Irena
authors.initials: I
authors.lastname: Kasacka
authors.affiliation: Department of Histology and Cytophysiology, Medical University, 15-222 Bialystok, Poland.
authors.forename: Zaneta
authors.initials: Z
authors.lastname: Piotrowska
authors.affiliation: Department of Nutrition and Food Assessment, Institute of Health Sciences, University of Natural Sciences and Humanities, 08-110 Siedlce, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Janiuk
authors.affiliation: 1 Clinical Department of General and Endocrine Surgery, University Clinical Hospital, 15-264 Bialystok, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Zbucki
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects* / Q000473:pathology; D000661:Amphetamine / Q000008:administration & dosage*; D000818:Animals; D003042:Cocaine / Q000008:administration & dosage*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006978:Hypertension, Renovascular / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D017208:Rats, Wistar; D013566:Sympathomimetics / Q000008:administration & dosage*; D013997:Time Factors; D014662:Vasoconstrictor Agents / Q000008:administration & dosage*; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: D009419:Nerve Tissue Proteins; D013566:Sympathomimetics; D014662:Vasoconstrictor Agents; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D000661:Amphetamine; D003042:Cocaine
keywords: Cocaine- and amphetamine-regulated transcript; adrenal glands; rat; renovascular hypertension