title: Cholesterol depletion induces ANTXR2-dependent activation of MMP-2 via ERK1/2 phosphorylation in neuroglioma U251 cell.
abstract: Cholesterol is a critical component of lipid rafts implicated in regulating multiple signal transduction. The anthrax toxin receptor 2 (ANTXR2) is a type I membrane protein acting as the second receptor for the anthrax toxin. In this study, we first investigated the association between cholesterol and ANTXR2. We provided the evidence that cholesterol depletion by methyl-beta-cyclodextrin (MβCD) promoted ANTXR2 expression in U251 neuroglioma cell, which was reversed by cholesterol supplement. MβCD-induced ANTXR2 up-regulation contributed to ERK1/2 phosphorylation, which was responsible for MT1-MMP and MMP-2 activation. Our data suggested that cellular cholesterol regulated ANTXR2-dependent activation of MMP-2 via ERK1/2 phosphorylation in neuroglioma U251 cell.
authors.affiliation: Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, PR China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Zou
authors.affiliation: Department of Clinical Laboratory, Zaozhuang Municipal Hospital, Zaozhuang, Shandong 277100, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ju
authors.affiliation: Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, PR China.
authors.forename: Peili
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, PR China. Electronic address: BingchangZhang2014@163.com.
authors.forename: Bingchang
authors.initials: B
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000378:metabolism*; D020868:Gene Silencing; D005910:Glioma / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020935:MAP Kinase Signaling System; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D010766:Phosphorylation; D018000:Receptors, Peptide / Q000235:genetics / Q000378:metabolism*
chemical_list: C447150:ANTXR2 protein, human; D018000:Receptors, Peptide; D002784:Cholesterol; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
keywords: ANTXR2; Cholesterol; ERK phosphorylation; MMP-2; MT1-MMP