title: Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts.
abstract: The present study provides a comprehensive data on the antioxidant, antimicrobial and neutrophil-modulating activities of extracts from six medicinal plants--blackberry (Rubus fruticosus) leaves, chokeberry (Aronia melanocarpa) leaves, hawthorn (Crataegus monogyna) leaves, lady's mantle (Alchemilla glabra) aerial parts, meadowsweet (Filipendula ulmaria) aerial parts and raspberry (Rubus idaeus) leaves. In order to analyze the antioxidant activity of the herbs, several methods (ORAC, TRAP, HORAC and inhibition of lipid peroxidation) were used. Blackberry leaves and meadowsweet extracts revealed the highest antioxidant activities via all methods. All extracts studied blocked almost completely the opsonized zymosan particle-activated ROS production by neutrophils from human whole blood. On the other hand, the effect of extracts on phorbol myristate acetate-activated ROS production was much milder and even nonsignificant in the case of chokeberry leaves. This latter result suggests that extracts (apart from their antioxidative activity) interfere with the signaling cascade of phagocyte activation upstream of the protein kinase C activation. The antimicrobial activity of the investigated extracts against 11 human pathogens was investigated using three different methods. Meadowsweet and blackberry leaves extracts had the highest antimicrobial effect and the lowest minimal inhibiting concentrations (MICs) against the microorganisms tested.
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Laboratory of Biologicaly Active Substances, Plovdiv, Bulgaria.
authors.forename: Petko
authors.initials: P
authors.lastname: Denev
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Laboratory of Biologicaly Active Substances, Plovdiv, Bulgaria.
authors.forename: Maria
authors.initials: M
authors.lastname: Kratchanova
authors.affiliation: Institute of Biophysics of the AS CR, Brno, Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Ciz
authors.affiliation: Institute of Biophysics of the AS CR, Brno, Czech Republic.
authors.forename: Antonin
authors.initials: A
authors.lastname: Lojek
authors.affiliation: Institute of Biophysics of the AS CR, Brno, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Vasicek
authors.affiliation: University of Food Technologies, Plovdiv, Bulgaria.
authors.forename: Denitsa
authors.initials: D
authors.lastname: Blazheva
authors.affiliation: University of Food Technologies, Plovdiv, Bulgaria.
authors.forename: Plamena
authors.initials: P
authors.lastname: Nedelcheva
authors.affiliation: Department of Animal Physiology and Immunology, Institute of Experimental Biology, Masaryk University, Brno, Czech Republic.
authors.forename: Libor
authors.initials: L
authors.lastname: Vojtek
authors.affiliation: Department of Animal Physiology and Immunology, Institute of Experimental Biology, Masaryk University, Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Hyrsl
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009895:Opsonin Proteins / Q000494:pharmacology; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D059808:Polyphenols / Q000302:isolation & purification / Q000494:pharmacology*; D061251:Primary Cell Culture; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D009895:Opsonin Proteins; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species; D013755:Tetradecanoylphorbol Acetate