title: Tissue inhibitor of metalloproteinase gene from pearl oyster Pinctada martensii participates in nacre formation.
abstract: Tissue inhibitors of metalloproteinases (TIMPs) are nature inhibitors of matrix metalloproteinases and play a vital role in the regulation of extracellular matrix turnover, tissue remodeling and bone formation. In this study, the molecular characterization of TIMP and its potential function in nacre formation was described in pearl oyster Pinctada martensii. The cDNA of TIMP gene in P. martensii (Pm-TIMP) was 901 bp long, containing a 5' untranslated region (UTR) of 51 bp, a 3' UTR of 169 bp, and an open reading fragment (ORF) of 681 bp encoding 226 amino acids with an estimated molecular mass of 23.37 kDa and a theoretical isoelectric point of 5.42; The predicted amino acid sequence had a signal peptide, 13 cysteine residues, a N-terminal domain and a C-terminal domain, similar to that from other species. Amino acid multiple alignment showed Pm-TIMP had the highest (41%) identity to that from Crassostrea gigas. Tissue expression analysis indicated Pm-TIMP was highly expressed in nacre formation related-tissues, including mantle and pearl sac. After decreasing Pm-TIMP gene expression by RNA interference (RNAi) technology in the mantle pallium, the inner nacreous layer of the shells showed a disordered growth. These results indicated that the obtained Pm-TIMP in this study participated in nacre formation.
authors.affiliation: Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang City, Guangdong 524025, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yan
authors.affiliation: Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang City, Guangdong 524025, China. Electronic address: jiaoyu1981@hotmail.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang City, Guangdong 524025, China. Electronic address: dengyw@gdou.edu.cn.
authors.forename: Yuewen
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang City, Guangdong 524025, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Du
authors.affiliation: Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang City, Guangdong 524025, China.
authors.forename: Ronglian
authors.initials: R
authors.lastname: Huang
authors.affiliation: Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang City, Guangdong 524025, China.
authors.forename: Qingheng
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang City, Guangdong 524025, China.
authors.forename: Weiyao
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D060734:Nacre / Q000502:physiology*; D009928:Organ Specificity / Q000502:physiology; D038081:Organogenesis / Q000502:physiology*; D049911:Pinctada / Q000502:physiology*; D013045:Species Specificity; D014018:Tissue Distribution; D019714:Tissue Inhibitor of Metalloproteinases / Q000145:classification / Q000235:genetics* / Q000378:metabolism*
chemical_list: D060734:Nacre; D019714:Tissue Inhibitor of Metalloproteinases
keywords: Nacre formation; Pinctada martensii; Tissue inhibitors of metalloproteinases (TIMPs)