title: Usefulness of broad-range PCR plus sequencing for the diagnosis of bacteremia due to a lung abscess.
abstract: The early detection and treatment of sepsis in patients is essential for a positive outcome. Microbiological analysis of blood cultures, as the gold standard for diagnosis, is rather slow. However, more rapid methods like PCR have become available recently and are being evaluated clinically. We present data from the monitoring of a patient with sepsis who was on anti-infective treatment. The patient was positive for Streptococcus pneumoniae by broad-range PCR and sequence analysis in a blood sample and resected lung tissue specimen, the latter embedded in paraffin, while blood culture diagnostics remained negative.
authors.affiliation: Department of Neurosurgery, Medical Centre Cologne-Merheim, Cologne, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Igressa
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, University Witten/Herdecke, Medical Centre Cologne-Merheim, Ostmerheimerstr. 200, D-51109 Cologne, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Defosse
authors.affiliation: Molzym GmbH & Co. KG, Bremen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Disqué
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, University Witten/Herdecke, Medical Centre Cologne-Merheim, Ostmerheimerstr. 200, D-51109 Cologne, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Wappler
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, University Witten/Herdecke, Medical Centre Cologne-Merheim, Ostmerheimerstr. 200, D-51109 Cologne, Germany. Electronic address: sakkas@kliniken-koeln.de.
authors.forename: S G
authors.initials: SG
authors.lastname: Sakka
mesh_terms: D000368:Aged; D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology*; D004269:DNA, Bacterial; D006801:Humans; D008169:Lung Abscess / Q000175:diagnosis* / Q000382:microbiology*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S; D013902:Radiography, Thoracic; D017422:Sequence Analysis, DNA; D014057:Tomography, X-Ray Computed
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Blood culture; Multiple trauma; Polymerase chain reaction; Sepsis