title: Inhibitory activity of the flower buds of Lonicera japonica Thunb. against histamine production and L-histidine decarboxylase in human keratinocytes.
abstract: In previous studies we found that anionic surfactants such as sodium laurate (SL) and/or sodium dodecylsulfate (SDS) exert actions on epidermal keratinocytes rather than mast cells to give rise of histamine production and skin itching through increasing the expression of the 53-kDa active form of L-histidine decarboxylase (HDC). In addition, with treatment of SL in a three-dimensional human keratinocyte culture, increases in both the 53-kDa HDC and histamine production are detected and thus this culture assay is applied to screen anti-itching materials from natural resources. In this study, the inhibitory activity of "Kin-gin-ka" (flower buds of Lonicera japonica Thunb., FLJ) against histamine production and expression of the active form of HDC were examined in this culture assay. FLJ is a well-known traditional Chinese medicine, being used to treat fevers, coughs and some infectious diseases. The result showed both FLJ and chlorogenic acid had inhibitory activities against the expression of 53-kDa HDC and histamine production. However, chlorogenic acid showed a weaker effect on histamine production than that of FLJ, suggesting that other chemical constituents besides chlorogenic acid could contribute to the inhibitory activities. Thus, a further chemical study of FLJ is now under investigation.
authors.affiliation: Fundamental Research Laboratories, Hoyu Co., Ltd., Roboku 1-12, Nagakute City Aichi 480-1136, Japan. yoshihiro_yinami@hoyu.co.jp.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Inami
authors.affiliation: Fundamental Research Laboratories, Hoyu Co., Ltd., Roboku 1-12, Nagakute City Aichi 480-1136, Japan. yuko_matsui@hoyu.co.jp.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Matsui
authors.affiliation: Fundamental Research Laboratories, Hoyu Co., Ltd., Roboku 1-12, Nagakute City Aichi 480-1136, Japan. tomoko_hoshino@hoyu.co.jp.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hoshino
authors.affiliation: Kampo Research Laboratories, Kracie Pharma., Ltd., Kanebo machi 3-1, Takaoka City Toyama 933-0856, Japan. murayama_chiaki@phm.kracie.co.jp.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Murayama
authors.affiliation: Kampo Research Laboratories, Kracie Pharma., Ltd., Kanebo machi 3-1, Takaoka City Toyama 933-0856, Japan. h_norimoto@phm.kracie.co.jp.
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Norimoto
mesh_terms: D002726:Chlorogenic Acid / Q000378:metabolism* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D006632:Histamine / Q000378:metabolism*; D006640:Histidine Decarboxylase / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism*; D007850:Lauric Acids / Q000494:pharmacology; D031239:Lonicera; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D007850:Lauric Acids; D010936:Plant Extracts; C030358:lauric acid; D002726:Chlorogenic Acid; D006632:Histamine; D006640:Histidine Decarboxylase