title: Synthesis of nucleobase-caged peptide nucleic acids having improved photochemical properties.
abstract: A nucleobase-caged peptide nucleic acid (PNA) having a (6-bromo-7-methoxycoumarin)-4-ylmethoxycarbonyl (Bmcmoc) caging group was newly synthesized. The Bmcmoc-caged PNAs were photolyzed to produce parent PNAs with a high photochemical efficiency. Introduction of a single Bmcmoc group was sufficient to suppress polymerase chain reaction (PCR) clamping activity and triplex invasion complex formation. Photo-mediated restoration of the PCR clamping activity was also demonstrated.
authors.affiliation: Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan. furuta@biomol.sci.toho-u.ac.jp.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hoshida
authors.forename: Jun
authors.initials: J
authors.lastname: Sakyo
authors.forename: Mariko
authors.initials: M
authors.lastname: Kishi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tanabe
authors.forename: Junichi
authors.initials: J
authors.lastname: Matsuura
authors.forename: Shingo
authors.initials: S
authors.lastname: Akiyama
authors.forename: Makiko
authors.initials: M
authors.lastname: Nakata
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Tanabe
authors.forename: Akinobu Z
authors.initials: AZ
authors.lastname: Suzuki
authors.forename: Soichiro
authors.initials: S
authors.lastname: Watanabe
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Furuta
mesh_terms: D003374:Coumarins / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D008027:Light; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D010782:Photolysis; D016133:Polymerase Chain Reaction; D011743:Pyrimidines / Q000737:chemistry*
chemical_list: D003374:Coumarins; D020135:Peptide Nucleic Acids; D011743:Pyrimidines