title: Arthrobacter bambusae sp. nov., isolated from soil of a bamboo grove.
abstract: A Gram-stain-positive, aerobic, motile by gliding, rod-shaped bacterial strain, THG-GM18(T), was isolated from soil of a bamboo grove. Strain THG-GM18(T) was able to grow in the presence of up to 6.0 % (w/v) NaCl, at 4-37 °C and at pH 7.0-10.0 in R2A medium. Based on 16S rRNA gene sequence similarity, strain THG-GM18(T) was closely related to species of the genus Arthrobacter. The most closely related strains to strain THG-GM18(T) are Arthrobacter ramosus CCM 1646(T) (98.5 % similarity), Arthrobacter nitroguajacolicus G2-1(T) (98.4 %), Arthrobacter nicotinovorans DSM 420(T) (98.2 %), Arthrobacter aurescens DSM 20116(T) (98.1 %) and Arthrobacter chlorophenolicus A6(T) (98.0 %). Strain THG-GM18(T) possessed chemotaxonomic properties consistent with those of members of the genus Arthrobacter, such as peptidoglycan type A3α (l-Lys-l-Ala-l-Thr-l-Ala), MK-9 as major menaquinone and anteiso- and iso-branched compounds (anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, unidentified phospholipids, unidentified aminolipids, an unidentified glycolipid and unidentified lipids. The G+C content of the genomic DNA was 61.0 mol%. The DNA-DNA relatedness values between strain THG-GM18(T) and its closest phylogenetic neighbours were below 26.0 %. The results of physiological and biochemical tests allowed the differentiation of strain THG-GM18(T) from species of the genus Arthrobacter with validly published names. Arthrobacter bambusae sp. nov. is the proposed name, and the type strain is THG-GM18(T) ( = KACC 17531(T) = JCM 19335(T)).
authors.affiliation: College of Life science, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: YongJin
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
authors.forename: MooChang
authors.initials: M
authors.lastname: Kook
authors.affiliation: College of Life science, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: Hien T T
authors.initials: HTT
authors.lastname: Ngo
authors.affiliation: College of Life science, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: Ki-Young
authors.initials: KY
authors.lastname: Kim
authors.affiliation: College of Life science, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Park
authors.affiliation: College of Life science, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: Gafurjon T
authors.initials: GT
authors.lastname: Mavlonov
authors.affiliation: College of Life science, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D001173:Arthrobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D031723:Bambusa / Q000382:microbiology*; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9