title: In vivo tracing uptake and elimination of organic pesticides in fish muscle.
abstract: Bioconcentration factors (BCFs) measured in the laboratory are important for characterizing the bioaccumulative properties of chemicals entering the environment, especially the potential persistent organic pollutants (POPs), which can pose serious adverse effects on ecosystem and human health. Traditional lethal analysis methods are time-consuming and sacrifice too many experimental animals. In the present study, in vivo solid-phase microextraction (SPME) was introduced to trace the uptake and elimination processes of pesticides in living fish. BCFs and elimination kinetic coefficients of the pesticides were recorded therein. Moreover, the metabolism of fenthion was also traced with in vivo SPME. The method was time-efficient and laborsaving. Much fewer experimental animals were sacrificed during the tracing. In general, this study opened up an opportunity to measure BCFs cheaply in laboratories for the registering of emerging POPs and inspecting of suspected POPs, as well as demonstrated the potential application of in vivo SPME in the study of toxicokinetics of pollutants.
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/KLGHEI of Environment and Energy Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University , Guangzhou, 510275 Guangdong, China.
authors.forename: Jianqiao
authors.initials: J
authors.lastname: Xu
authors.forename: Junpeng
authors.initials: J
authors.lastname: Luo
authors.forename: Jingwen
authors.initials: J
authors.lastname: Ruan
authors.forename: Fang
authors.initials: F
authors.lastname: Zhu
authors.forename: Tiangang
authors.initials: T
authors.lastname: Luan
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Ruifen
authors.initials: R
authors.lastname: Jiang
authors.forename: Gangfeng
authors.initials: G
authors.lastname: Ouyang
mesh_terms: D000818:Animals; D004784:Environmental Monitoring; D005284:Fenthion / Q000378:metabolism; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D009930:Organic Chemicals / Q000378:metabolism*; D010575:Pesticides / Q000378:metabolism*; D052617:Solid Phase Microextraction; D017210:Tilapia / Q000378:metabolism*
chemical_list: D006843:Hydrocarbons, Chlorinated; D009930:Organic Chemicals; D010575:Pesticides; D005284:Fenthion