title: MicroRNA-141 promotes the proliferation of non-small cell lung cancer cells by regulating expression of PHLPP1 and PHLPP2.
abstract: The dysregulation of microRNAs (miRNAs) is crucially implicated in the development of various cancers. In this study, we explored the biological role of miR-141 in non-small cell lung cancer (NSCLC). miR-141 expression was significantly up-regulated in NSCLC tissues, and its overexpression accelerated NSCLC cell proliferation in vitro and tumor growth in vivo. We subsequently identified the antagonists of PI3K/AKT signaling, PH domain leucine-rich-repeats protein phosphatase 1 (PHLPP1) and PHLPP2, as direct targets of miR-141. Re-introduction of PHLPP1 and PHLPP2 abrogated miR-141-induced proliferation of NSCLC cells. Together, the results of this study suggest that miR-141 and its targets PHLPP1 and PHLPP2 play critical roles in NSCLC tumorigenesis, and provide potential therapeutic targets for NSCLC treatment.
authors.affiliation: Department of Respiratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
authors.forename: Zhoufang
authors.initials: Z
authors.lastname: Mei
authors.affiliation: Department of Respiratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
authors.forename: Yanchao
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Respiratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Respiratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Respiratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Respiratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Qian
authors.affiliation: Department of Respiratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Respiratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China. Electronic address: zjjiefudan@163.com.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Jie
mesh_terms: D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C533895:MIRN141 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; C400601:PHLPP1 protein, human; C541572:PHLPP2 protein, human; D010749:Phosphoprotein Phosphatases
keywords: MicroRNA-141; Non-small cell lung cancer; PH domain leucine-rich-repeats protein phosphatase 1; PH domain leucine-rich-repeats protein phosphatase 2; Proliferation