title: Immobilization of Trichoderma harzianum α-amylase on treated wool: optimization and characterization.
abstract: α-Amylase from Trichoderma harzianum was covalently immobilized on activated wool by cyanuric chloride. Immobilized α-amylase exhibited 75% of its initial activity after 10 runs. The soluble and immobilized α-amylases exhibited maximum activity at pH values 6.0 and 6.5, respectively. The immobilized enzyme was more thermally stable than the soluble one. Various substrates were hydrolyzed by immobilized α-amylase with high efficiencies compared to those of soluble α-amylase. The inhibition of the immobilized α-amylase by metal ions was low as compared with soluble enzyme. On the basis of the results obtained, immobilized α-amylase could be employed in the saccharification of starch processing.
authors.affiliation: Biochemistry Department, Faculty of Science, King Abdulaziz University, 21589, Jeddah, Kingdom of Saudi Arabia. saleh38@hotmail.com.
authors.forename: Saleh A
authors.initials: SA
authors.lastname: Mohamed
authors.affiliation: Biochemistry Department, Faculty of Science, King Abdulaziz University, 21589, Jeddah, Kingdom of Saudi Arabia. jalalawlia@yahoo.com.
authors.forename: Jalaluddin A
authors.initials: JA
authors.lastname: Khan
authors.affiliation: Biochemistry Department, Faculty of Science, King Abdulaziz University, 21589, Jeddah, Kingdom of Saudi Arabia. al_baromar@hotmail.com.
authors.forename: Omar A M
authors.initials: OA
authors.lastname: Al-Bar
authors.affiliation: Chemistry Department, Faculty of Science, King Abdulaziz University, 21589, Jeddah, Kingdom of Saudi Arabia. elshishtawy@hotmail.com.
authors.forename: Reda M
authors.initials: RM
authors.lastname: El-Shishtawy
mesh_terms: D000818:Animals; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D014242:Trichoderma / Q000201:enzymology*; D014935:Wool / Q000737:chemistry*; D000516:alpha-Amylases / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D000516:alpha-Amylases