title: Expression of serum amyloid A4 in human trophoblast-like choriocarcinoma cell lines and human first trimester/term trophoblast cells.
abstract: Trophoblast invasion into uterine tissues represents a hallmark of first trimester placental development. As expression of serum amyloid A4 (SAA4) occurs in tumorigenic and invasive tissues we here investigated whether SAA4 is present in trophoblast-like human AC1-M59/Jeg-3 cells and trophoblast preparations of human first trimester and term placenta. SAA4 mRNA was expressed in non-stimulated and cytokine-treated AC1-M59/Jeg-3 cells. In purified trophoblast cells SAA4 mRNA expression was upregulated at weeks 10 and 12 of pregnancy. Western-blot and immunohistochemical staining of first trimester placental tissue revealed pronounced SAA4 expression in invasive trophoblast cells indicating a potential role of SAA4 during invasion.
authors.affiliation: Medical University of Graz, Institute of Molecular Biology and Biochemistry, Graz A-8010, Austria.
authors.forename: C
authors.initials: C
authors.lastname: Rossmann
authors.affiliation: Medical University of Graz, Institute of Cell Biology, Histology and Embryology, Graz, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Hammer
authors.affiliation: Medical University of Graz, Institute of Molecular Biology and Biochemistry, Graz A-8010, Austria.
authors.forename: C N
authors.initials: CN
authors.lastname: Koyani
authors.affiliation: Medical University of Graz, Institute of Molecular Biology and Biochemistry, Graz A-8010, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Kovacevic
authors.affiliation: Medical University of Graz, Institute of Cell Biology, Histology and Embryology, Graz, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Siwetz
authors.affiliation: Medical University of Graz, Department of Obstetrics and Gynecology, Graz, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Desoye
authors.affiliation: Placenta-Laboratory, Department of Obstetrics, University Hospital Jena, Jena, Germany.
authors.forename: T G
authors.initials: TG
authors.lastname: Poehlmann
authors.affiliation: Placenta-Laboratory, Department of Obstetrics, University Hospital Jena, Jena, Germany.
authors.forename: U R
authors.initials: UR
authors.lastname: Markert
authors.affiliation: Medical University of Graz, Institute of Cell Biology, Histology and Embryology, Graz, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Huppertz
authors.affiliation: Medical University of Graz, Institute of Molecular Biology and Biochemistry, Graz A-8010, Austria.
authors.forename: W
authors.initials: W
authors.lastname: Sattler
authors.affiliation: Medical University of Graz, Institute of Molecular Biology and Biochemistry, Graz A-8010, Austria. Electronic address: ernst.malle@medunigraz.at.
authors.forename: E
authors.initials: E
authors.lastname: Malle
mesh_terms: D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: C094771:SAA4 protein, human; D000685:Serum Amyloid A Protein
keywords: Cytokine; Invasion; Outgrowing extravillous trophoblast; Placenta; SAA4