title: Photodegradable hydrogels for capture, detection, and release of live cells.
abstract: Cells may be captured and released using a photodegradable hydrogel (photogel) functionalized with antibodies. Photogel substrates were used to first isolate human CD4 or CD8 T-cells from a heterogeneous cell suspension and then to release desired cells or groups of cells by UV-induced photodegradation. Flow cytometry analysis of the retrieved cells revealed approximately 95% purity of CD4 and CD8 T-cells, suggesting that this substrate had excellent specificity. To demonstrate the possibility of sorting cells according to their function, photogel substrates that were functionalized with anti-CD4 and anti-TNF-α antibodies were prepared. Single cells captured and stimulated on such substrates were identified by the fluorescence "halo" after immunofluorescent staining and could be retrieved by site-specific exposure to UV light through a microscope objective. Overall, it was demonstrated that functional photodegradable hydrogels enable the capture, analysis, and sorting of live cells.
authors.affiliation: Department of Biomedical Engineering, University of California Davis, One Shields Ave, Davis, CA 95616 (USA). dshin@ucdavis.edu.
authors.forename: Dong-Sik
authors.initials: DS
authors.lastname: Shin
authors.forename: Jungmok
authors.initials: J
authors.lastname: You
authors.forename: Ali
authors.initials: A
authors.lastname: Rahimian
authors.forename: Tam
authors.initials: T
authors.lastname: Vu
authors.forename: Christian
authors.initials: C
authors.lastname: Siltanen
authors.forename: Arshia
authors.initials: A
authors.lastname: Ehsanipour
authors.forename: Gulnaz
authors.initials: G
authors.lastname: Stybayeva
authors.forename: Julie
authors.initials: J
authors.lastname: Sutcliffe
authors.forename: Alexander
authors.initials: A
authors.lastname: Revzin
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D006801:Humans; D020100:Hydrogels; D055668:Photochemical Processes; D014466:Ultraviolet Rays
chemical_list: D020100:Hydrogels
keywords: antibodies; hydrogels; microarrays; micropatterning; single-cell detection