title: JK1 (FAM134B) represses cell migration in colon cancer: a functional study of a novel gene.
abstract: BACKGROUND
  JK1 is a novel cancer-related gene with unknown functional role in carcinogenesis. The aim of this study is to investigate the role of JK1 gene in carcinogenesis in an in vitro cell proliferation and migration analysis model.
  
  
  METHODS
  Small hairpin RNAs (shRNA) were designed to knock-down JK1 expression in colon cancer cell line (SW480) using transduction ready lentiviral particles. Cell proliferation and cell migration assays were performed on multiple extracellular matrices to investigate the cellular effects of JK1 in colon cancer cells. A non-cancer colonic epithelial cell line (FHC) was used to compare the expression of JK1 in cancer cell line.
  
  
  RESULTS
  JK1 knock-down did not affect cellular proliferation or survival in colon cancer. However, the manipulation increased cancer cell migration rates on collagen and fibronectin substrates.
  
  
  CONCLUSIONS
  JK1 was shown for the first time to have a functional role in the pathogenesis of colon cancer. The results imply that JK1 represses the capacity of cancer cells to migrate within their tissue. They also concurred with the previous findings of JK1 activity correlations with clinical and pathological features in colon cancer. The capacity may have utility as a means to prevent cancer cells forming metastases.
authors.affiliation: Cancer Molecular Pathology of Griffith Health Institute, Griffith University, Gold Coast, Queensland, Australia.
authors.forename: Kais
authors.initials: K
authors.lastname: Kasem
authors.affiliation: Cancer Molecular Pathology of Griffith Health Institute, Griffith University, Gold Coast, Queensland, Australia.
authors.forename: Emily
authors.initials: E
authors.lastname: Sullivan
authors.affiliation: Cancer Molecular Pathology of Griffith Health Institute, Griffith University, Gold Coast, Queensland, Australia.
authors.forename: Vinod
authors.initials: V
authors.lastname: Gopalan
authors.affiliation: Cancer Molecular Pathology of Griffith Health Institute, Griffith University, Gold Coast, Queensland, Australia.
authors.forename: Ali
authors.initials: A
authors.lastname: Salajegheh
authors.affiliation: Cancer Molecular Pathology of Griffith Health Institute, Griffith University, Gold Coast, Queensland, Australia.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Smith
authors.affiliation: Cancer Molecular Pathology of Griffith Health Institute, Griffith University, Gold Coast, Queensland, Australia. Electronic address: a.lam@griffith.edu.au.
authors.forename: Alfred K-Y
authors.initials: AK
authors.lastname: Lam
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C522691:RETREG1 protein, human; D034741:RNA, Small Interfering
keywords: Colon cancer; FAM134B; JK1; Migration