title: miR-365 promotes cutaneous squamous cell carcinoma (CSCC) through targeting nuclear factor I/B (NFIB).
abstract: Aberrant expression of microRNAs plays vital roles in tumor development and progression. As transcription factors (TFs) are the critical components of signaling cascades, specific targeting effects of microRNAs to specific TFs may determine the role of microRNAs in different cancers. In this study, we identified Nuclear Factor I/B (NFIB) as one of the targets of miR-365 which was previously verified as an onco-miR in cutaneous squamous cell carcinoma (CSCC). Down-regulation of NFIB was a general feature in both CSCC cell lines and tumors from patients which show drastically up-regulated miR-365 expression levels. The siRNA-based knockdown of NFIB mimic the carcinogenic transformation of normal cells by ectopically expression of miR-365 which indicates depletion of NFIB is necessary for miR-365 to exert its pro-carcinogenic function. NFIB may represent a functional barrier targeted by miR-365 to the development of CSCC. Further studies also discovered a conserved feedback regulatory circuitry formed by NFIB and miR-365 in CSCC development which may be potentially utilized as therapeutic target to improve the clinical CSCC treatment.
authors.affiliation: Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangdong, Guangzhou, People's Republic of China.
authors.forename: Meijuan
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangdong, Guangzhou, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangdong, Guangzhou, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangdong, Guangzhou, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangdong, Guangzhou, People's Republic of China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangdong, Guangzhou, People's Republic of China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangdong, Guangzhou, People's Republic of China.
authors.forename: Chengshan
authors.initials: C
authors.lastname: Ou
authors.affiliation: Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangdong, Guangzhou, People's Republic of China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Ding
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D051839:NFI Transcription Factors / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000235:genetics; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: C546819:MIRN365 microRNA, human; D035683:MicroRNAs; D051839:NFI Transcription Factors; C110754:NFIB protein, human; D034741:RNA, Small Interfering