title: Acute and delayed deferoxamine treatment attenuates long-term sequelae after germinal matrix hemorrhage in neonatal rats.
abstract: OBJECTIVE
  This study investigated if acute and delayed deferoxamine treatment attenuates long-term sequelae after germinal matrix hemorrhage (GMH).
  
  
  METHODS
  Bacterial collagenase (0.3 U) was infused intraparenchymally into the right hemispheric ganglionic eminence in P7 rat pups to induce GMH. GMH animals received either deferoxamine or vehicle twice a day for 7 consecutive days. Deferoxamine administration was initiated at either 1 hour or 72 hours post-GMH. Long-term neurocognitive deficits and motor coordination were assessed using Morris water maze, rotarod, and foot fault tests between day 21 to 28 post-GMH. At 28 days post-GMH, brain morphology was assessed and extracellular matrix protein (fibronectin and vitronectin) expression was determined.
  
  
  RESULTS
  Acute and delayed deferoxamine treatment improved long-term motor and cognitive function at 21 to 28 days post-GMH. Attenuated neurofunction was paralleled with improved overall brain morphology at 28 days post-GMH, reducing white matter loss, basal ganglia loss, posthemorrhagic ventricular dilation, and cortical loss. GMH resulted in significantly increased expression of fibronectin and vitronectin, which was reversed by acute and delayed deferoxamine treatment.
  
  
  CONCLUSIONS
  Acute and delayed deferoxamine administration ameliorated long-term sequelae after GMH.
authors.affiliation: From the Department of Physiology and Pharmacology, Loma Linda University School of Medicine, CA.
authors.forename: Damon
authors.initials: D
authors.lastname: Klebe
authors.affiliation: From the Department of Physiology and Pharmacology, Loma Linda University School of Medicine, CA.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Krafft
authors.affiliation: From the Department of Physiology and Pharmacology, Loma Linda University School of Medicine, CA.
authors.forename: Clotilde
authors.initials: C
authors.lastname: Hoffmann
authors.affiliation: From the Department of Physiology and Pharmacology, Loma Linda University School of Medicine, CA.
authors.forename: Tim
authors.initials: T
authors.lastname: Lekic
authors.affiliation: From the Department of Physiology and Pharmacology, Loma Linda University School of Medicine, CA.
authors.forename: Jerry J
authors.initials: JJ
authors.lastname: Flores
authors.affiliation: From the Department of Physiology and Pharmacology, Loma Linda University School of Medicine, CA.
authors.forename: William
authors.initials: W
authors.lastname: Rolland
authors.affiliation: From the Department of Physiology and Pharmacology, Loma Linda University School of Medicine, CA. johnzhang3910@yahoo.com.
authors.forename: John H
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects*; D003676:Deferoxamine / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D020300:Intracranial Hemorrhages / Q000188:drug therapy* / Q000503:physiopathology; D018782:Maze Learning / Q000187:drug effects* / Q000502:physiology; D009043:Motor Activity / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D013997:Time Factors
chemical_list: D003676:Deferoxamine
keywords: deferoxamine; extracellular matrix proteins