title: Effect of complement C1q expression on hepatic ischemia-reperfusion injury in rats.
abstract: The effect of the complement C1q expression on total hepatic ischemia-reperfusion (I/R) injury in rats was investigated. Sixty healthy male Sprague Dawley (SD) rats weighing 180-200 g were randomly divided into 5 groups: sham-operation group (S group, n=12); group of I/R for 1 h (I/R 1 h group, n=12); group of I/R for 3 h (I/R 3 h group, n=12); group of I/R for 6 h (I/R 6 h group, n=12); group of I/R for 24 h (I/R 24 h group, n=12). The hepatic I/R model of rats was established, and liver tissues were obtained 1 h, 3 h, 6 h and 24 h after hepatic I/R, respectively. Furthermore, the tissues were stained using hematoxylin-eosin, and the liver injuries of rats were observed using a microscope. The malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in liver tissue were determined. Real-time polymerase chain reaction (PCR) and Western blotting were used to detect the expression levels of C1q mRNA and protein, respectively. As compared with the S group, the histopathological changes in I/R 1 h-24 h groups were gradually aggravated with the extension of I/R time. As compared with the S group, SOD activity and MDA content in the I/R groups were reduced and increased respectively with the extension of I/R time (P<0.01). Furthermore, the C1q expression at mRNA and protein levels in the I/R groups (especially in the I/R 3 h group) was significantly higher than that in the S group (P<0.05). It is suggested that C1q expression may play a principal role in hepatic I/R injury, particularly at the early stage of perfusion.
authors.affiliation: Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Feng
authors.affiliation: Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China. kjj428@sohu.com.
authors.forename: Jian-Juan
authors.initials: JJ
authors.lastname: Ke
authors.affiliation: Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Rao
authors.affiliation: Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
authors.forename: Zong-Ze
authors.initials: ZZ
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
authors.forename: Yan-Lin
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015922:Complement C1q / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008099:Liver / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
chemical_list: D008315:Malondialdehyde; D015922:Complement C1q; D013482:Superoxide Dismutase