title: Effect of two different preparations of platelet-rich plasma on synoviocytes.
abstract: OBJECTIVE
  To analyse the modifications induced by two different platelet-rich plasma (PRP) preparations on osteoarthritis (OA) synoviocytes, by documenting changes in gene expression of factors involved in joint physiopathology.
  
  
  METHODS
  OA synoviocytes were cultured for 7 days in medium with different concentrations of either P-PRP (a pure platelet concentrate without leucocytes but with a limited number of platelets), L-PRP (a higher platelet concentrate containing leucocytes) or platelet-poor plasma (PPP). Gene expression of interleukin (IL)-1beta, IL-6, IL-8/CXCL8, tumour necrosis factor alpha, IL-10, IL-4, IL-13, metalloproteinase-13, tissue inhibitor of metalloproteinase (TIMP)-1, (TIMP)-3, (TIMP)-4, vascular endothelial growth factor, transforming growth factor beta1, fibroblast growth factor (FGF)-2, hepatocyte growth factor (HGF), hyaluronic acid (HA) synthases (HAS)-1, (HAS)-2, and (HAS)-3 was analysed by RT-PCR. HA production was determined in culture supernatants by ELISA.
  
  
  RESULTS
  IL-1β, IL-8 and FGF-2 were significantly induced by L-PRP compared to both P-PRP and PPP; HGF was down-modulated by L-PRP versus both P-PRP and PPP, and an inverse dose-response influence was shown for all preparations. Expression level of TIMP-4 was lower in the presence of L-PRP compared with P-PRP. HA production and HAS gene expression did not seem to be modulated by PRP.
  
  
  CONCLUSIONS
  L-PRP is able to sustain the up-regulation of proinflammatory factors, (IL-1beta, IL-8 and FGF-2), together with a down-modulation of HGF and TIMP-4 expression, two factors that have been recognized as anti-catabolic mediators in cartilage, thus supporting the need to further optimize the PRP preparations to be applied in clinical practice.
authors.affiliation: Laboratory of Immunorheumatology and Tissue Regeneration/RAMSES, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy, elisa.assirelli@ior.it.
authors.forename: Elisa
authors.initials: E
authors.lastname: Assirelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Filardo
authors.forename: Erminia
authors.initials: E
authors.lastname: Mariani
authors.forename: Elizaveta
authors.initials: E
authors.lastname: Kon
authors.forename: Alice
authors.initials: A
authors.lastname: Roffi
authors.forename: Franca
authors.initials: F
authors.lastname: Vaccaro
authors.forename: Maurilio
authors.initials: M
authors.lastname: Marcacci
authors.forename: Andrea
authors.initials: A
authors.lastname: Facchini
authors.forename: Lia
authors.initials: L
authors.lastname: Pulsatelli
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000276:immunology; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology; D008297:Male; D020370:Osteoarthritis, Knee / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D053657:Platelet-Rich Plasma / Q000276:immunology*; D013583:Synovial Membrane / Q000276:immunology* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D036341:Intercellular Signaling Peptides and Proteins