title: Effects of metoprolol and small intestine RNA on marrow-derived endothelial progenitor cells applied for autograft transplantation in heart disease.
abstract: OBJECTIVE
  The objective of this project was to improve the effect of EPC autograft transplantation and observe the tolerance of EPCs to I/R injury affected by metoprolol and small intestine RNA.
  
  
  METHODS
  We isolated bone marrow-derived EPCs and examined the effects of metoprolol and small intestine RNA on EPCs to ischemia at different time points after reperfusion. EPCs growth curve, secretion, apoptosis and mortality were also analyzed.
  
  
  RESULTS
  EPCs will be better protected if the blood can be recovered within 4 hours after ischemia for cardiac muscle cells and pretreatment of EPCs with metoprolol or small intestine RNA could protect and promote EPCs proliferation.
  
  
  CONCLUSIONS
  Our study demonstated that pretreatment of EPCs with metoprolol or small intestine RNA will increase the EPCs proliferation and may improve the EPCs autograft transplantation ability.
authors.affiliation: Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. chengkang2009@foxmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Cheng
authors.forename: M-Q
authors.initials: MQ
authors.lastname: Wei
authors.forename: G-L
authors.initials: GL
authors.lastname: Jia
authors.forename: H-C
authors.initials: HC
authors.lastname: Wang
authors.forename: R-H
authors.initials: RH
authors.lastname: Luan
authors.forename: W-Y
authors.initials: WY
authors.lastname: Guo
authors.forename: W-J
authors.initials: WJ
authors.lastname: Li
authors.forename: X-J
authors.initials: XJ
authors.lastname: Zong
authors.forename: X
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology*; D042783:Endothelial Cells / Q000166:cytology*; D007421:Intestine, Small / Q000737:chemistry; D045022:Lactate Dehydrogenases / Q000378:metabolism; D008297:Male; D008790:Metoprolol / Q000494:pharmacology*; D023421:Models, Animal; D009203:Myocardial Infarction / Q000628:therapy*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D012313:RNA / Q000494:pharmacology*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*; D013552:Swine; D013556:Swine, Miniature; D014182:Transplantation, Autologous
chemical_list: D009569:Nitric Oxide; D012313:RNA; D045022:Lactate Dehydrogenases; D052247:Nitric Oxide Synthase Type II; D008790:Metoprolol