title: Automated peptide mapping and protein-topographical annotation of proteomics data.
abstract: BACKGROUND
  In quantitative proteomics, peptide mapping is a valuable approach to combine positional quantitative information with topographical and domain information of proteins. Quantitative proteomic analysis of cell surface shedding is an exemplary application area of this approach.
  
  
  RESULTS
  We developed ImproViser ( http://www.improviser.uni-freiburg.de) for fully automated peptide mapping of quantitative proteomics data in the protXML data. The tool generates sortable and graphically annotated output, which can be easily shared with further users. As an exemplary application, we show its usage in the proteomic analysis of regulated intramembrane proteolysis.
  
  
  CONCLUSIONS
  ImproViser is the first tool to enable automated peptide mapping of the widely-used protXML format.
authors.forename: Pavankumar
authors.initials: P
authors.lastname: Videm
authors.forename: Deepika
authors.initials: D
authors.lastname: Gunasekaran
authors.forename: Bernd
authors.initials: B
authors.lastname: Schröder
authors.forename: Bettina
authors.initials: B
authors.lastname: Mayer
authors.forename: Martin L
authors.initials: ML
authors.lastname: Biniossek
authors.affiliation: Institute of Molecular Medicine and Cell Research, University of Freiburg, D-79104 Freiburg, Germany. oliver.schilling@mol-med.uni-freiburg.de.
authors.forename: Oliver
authors.initials: O
authors.lastname: Schilling
mesh_terms: D057205:Automation, Laboratory; D006801:Humans; D058977:Molecular Sequence Annotation / Q000379:methods*; D010449:Peptide Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics
chemical_list: D011506:Proteins