title: Development and validation of high liquid performance chromatography-tandem mass spectrometry method for simultaneous determination of geniposidic acid and aucubin in rat plasma for pharmacokinetic study after oral administration of Du-zhong tea extract.
abstract: A specific and sensitive high performance liquid chromatography coupled with tandem mass spectrometric (HPLC-MS/MS) method was developed and validated for the simultaneous determination of geniposidic acid and aucubin in rat plasma after oral administration of Du-zhong tea extract. The plasma samples were pretreated by protein precipitation with methanol and the chromatographic separation was performed on a Hypersil C18 column (4.6 mm×250 mm, 5 μm), using a gradient mobile phase system of water-methanol (0.05% formic acid). The detection was accomplished by multiple-reaction monitoring (MRM) scanning via electrospray ionization source operating in the negative ionization mode. The linear range was 1-1,000 ng/mL for geniposidic acid and 0.2-200 ng/mL for aucubin, respectively. The accuracy (relative error, R.E.%) were between -5.40 and 5.00%, while the intra-day and inter-day precisions were less than 7.95 and 7.87% for the two analytes, respectively. The method was fully validated for the sensitivity, selectivity, recovery, matrix effect and stability. Then this method was successfully applied to the pharmacokinetic study of geniposidic acid and aucubin after oral administration of Du-zhong tea extract to rats and the results indicated that this HPLC-MS/MS assay is a valuable method for the pharmacokinetic study of geniposidic acid and aucubin in rat plasma.
authors.affiliation: State Engineering Laboratory of Bio-Resources Eco-Utilization, Northeast Forestry University, No. 26 Hexing Road Xiangfang District, Harbin 150040, China; Engineering Research Center of Forestry Bio-preparation, Ministry of Education, Northeast Forestry University, No. 26 Hexing Road Xiangfang District, Harbin 150040, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, No. 26 Hexing Road Xiangfang District, Harbin 150040, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Heilongjiang University of Technology, No. 99 Hepingnan Road Jiguan District, Jixi 158100, China.
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Ma
authors.affiliation: State Engineering Laboratory of Bio-Resources Eco-Utilization, Northeast Forestry University, No. 26 Hexing Road Xiangfang District, Harbin 150040, China; Engineering Research Center of Forestry Bio-preparation, Ministry of Education, Northeast Forestry University, No. 26 Hexing Road Xiangfang District, Harbin 150040, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, No. 26 Hexing Road Xiangfang District, Harbin 150040, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Engineering Laboratory of Bio-Resources Eco-Utilization, Northeast Forestry University, No. 26 Hexing Road Xiangfang District, Harbin 150040, China; Engineering Research Center of Forestry Bio-preparation, Ministry of Education, Northeast Forestry University, No. 26 Hexing Road Xiangfang District, Harbin 150040, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, No. 26 Hexing Road Xiangfang District, Harbin 150040, China. Electronic address: zuyuangang@163.com.
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000493:pharmacokinetics; D031283:Eucommiaceae / Q000737:chemistry*; D057888:Iridoid Glucosides / Q000097:blood*; D008297:Male; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D057888:Iridoid Glucosides; C058966:geniposidic acid; C006650:aucubin
keywords: Aucubin; Du-zhong tea; Geniposidic acid; HPLC-MS/MS; Pharmacokinetics; Rat plasma