title: Changes of c-fos, malondialdehyde and lactate in brain tissue after global cerebral ischemia under different brain temperatures.
abstract: Under global cerebral ischemia, the effect of different brain temperature on cerebral ischemic injury was studied. Male Sprague-Dawley rats were divided into normothermic (37-38°C) ischemia, mild hypothermic (31-32°C) ischemia, hyperthermic (41-42°C) ischemia and sham-operated groups. Global cerebral ischemia was established using the Pulsinelli four-vessel occlusion model and brain temperature was maintained at defined level for 60 min after 20-min ischemia. The expression of c-fos protein and the levels of malondialdehyde (MDA) and lactate in brain regions were detected by immunochemistry and spectrophotometrical methods, respectively. C-fos positive neurons were found in the hippocampus and cerebral cortex after cerebral ischemia reperfusion. Mild hypothermia increased the expression of c-fos protein in both areas, whereas hyperthermia decreased the expression of c-fos protein in the hippocampus at 24 h reperfusion, and the cerebral cortex at 48 h reperfusion when compared to normothermic conditions. In normothermic, mild hypothermic and hyperthermic ischemia groups, the levels of MDA and lactate in brain tissue were increased at 24, 48 and 72 h reperfusion following 20-min ischemia as compared with the sham-operated group (P<0.01). The levels of MDA and lactate in mild hypothermic group were significantly lower than those in normothermic group (P<0.01). It is suggested that brain temperature influences the translation of the immunoreactive protein product of c-fos after global cerebral ischemia, and MDA and lactate are also affected by hypothermia and hyperthermia.
authors.affiliation: Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China. zhangh9@yahoo.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China.
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Xu
authors.affiliation: Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Yuan-Wu
authors.initials: YW
authors.lastname: Mei
authors.affiliation: Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors.forename: Jun-Jian
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China.
authors.forename: Shen-Xing
authors.initials: SX
authors.lastname: Murong
authors.affiliation: Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Sheng-Gang
authors.initials: SG
authors.lastname: Sun
authors.affiliation: Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: E-Tang
authors.initials: ET
authors.lastname: Tong
mesh_terms: D000818:Animals; D001831:Body Temperature; D001921:Brain / Q000098:blood supply / Q000378:metabolism* / Q000503:physiopathology; D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology; D006624:Hippocampus / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology; D007120:Immunochemistry; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D016760:Proto-Oncogene Proteins c-fos; D016159:Tumor Suppressor Protein p53; D019344:Lactic Acid; D008315:Malondialdehyde