title: Deep sequencing analysis of microRNA expression in porcine serum-induced hepatic fibrosis rats.
abstract: OBJECTIVE
  Recent studies have suggested miRNA dysregulation in liver tissue mediates the pathogenesis of various liver diseases especially liver fibrosis, but the microRNA changes during PS-induced hepatic fibrosis are still unknown. The purpose of this study was to screen the miRNA differences in rat liver fibrosis model and clarify the relationship of miRNAs with the development of PS-induced liver fibrosis.
  
  
  METHODS
  Two fibrotic and two normal liver tissues from 20 Sprague-Dawley rats were collected and sequenced. MiRNA profiling results and fibrosis-related genes were validated by quantitative real-time polymerase chain reaction (qRT-PCR) and bioinformatics was used to predict miRNA targets.
  
  
  RESULTS
  In total, 48 miRNAs were detected to be aberrantly expressed in fibrosis tissue compared to normal tissue. Further functional analysis of the deregulated miRNA targets revealed the miRNAs are involved in several biological functions and pathways. In addition, the expression level of miR-27a and miR-146b and fibrosis-related genes were significantly up-regulated by using qRT-PCR in fibrotic liver tissues when compared to the normal liver tissues.
  
  
  CONCLUSIONS
  PS-induced hepatic fibrosis results in up-regulation of the miR-27a and miR-146b in liver tissues, suggestingmiR-27a and miR-146b would be associated with the development of PS-induced liver fibrosis and be potential therapeutic targets during hepatic fibrosis.
authors.affiliation: Department of Infectious Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Shanfei
authors.initials: S
authors.lastname: Ge
authors.affiliation: BGI-tianjin, Tianjin, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Infectious Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
authors.affiliation: Bioinformatics Center, Beijing Genomics Institute, Shenzhen, Guangdong, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yi
authors.affiliation: BGI-tianjin, Tianjin, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008106:Liver Cirrhosis, Experimental / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA / Q000379:methods*; D044967:Serum; D013552:Swine; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs