title: A "turn on/off" scorpion biosensor targeting point mutation of SMN genes for diagnosis of spinal muscular atrophy.
abstract: A "turn on/off" biosensor for diagnosis of exon 7 of the SMN1 gene was developed by employing a "scorpion primer". This scorpion primer was based on the principle of fluorescence resonance energy transfer using a fluorophore, a blocker and a quencher. It was successfully applied to detect 10 volunteer samples, and not only to in vitro testing.
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan. shmewu@kmu.edu.tw.
authors.forename: Chung-An
authors.initials: CA
authors.lastname: Chen
authors.forename: Chun-Chi
authors.initials: CC
authors.lastname: Wang
authors.forename: Yen-Ling
authors.initials: YL
authors.lastname: Chen
authors.forename: Yuh-Jyh
authors.initials: YJ
authors.lastname: Jong
authors.forename: Shou-Mei
authors.initials: SM
authors.lastname: Wu
mesh_terms: D015374:Biosensing Techniques; D005091:Exons / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D009134:Muscular Atrophy, Spinal / Q000175:diagnosis* / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D055533:Survival of Motor Neuron 1 Protein / Q000235:genetics*
chemical_list: C526471:SMN1 protein, human; D055533:Survival of Motor Neuron 1 Protein