title: Development of a simplified and convenient assay for cell-mediated immunity to the mumps virus.
abstract: Because methods for measuring cell-mediated immunity (CMI) to the mumps virus are expensive, time-consuming, and technically demanding, the role of CMI in mumps virus infection remains unclear. To address this issue, we report here the development of a simplified method for measuring mumps virus-specific CMI that is suitable for use in diverse laboratory and clinical settings. A mumps vaccine was cultured with whole blood, and interferon (IFN)-γ released into the culture supernatant was measured using an enzyme-linked immunosorbent assay. IFN-γ production in blood from vaccinated subjects markedly increased in response to the vaccine and decreased before the antibody titer decreased in some cases, suggesting that this assay may be used as a simple surrogate method for measuring CMI specific for the mumps virus.
authors.affiliation: Department of Public Health, Hyogo College of Medicine, Nishinomiya, Japan.
authors.forename: Naruhito
authors.initials: N
authors.lastname: Otani
authors.affiliation: Department of Public Health, Hyogo College of Medicine, Nishinomiya, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shima
authors.affiliation: Department of Infection Control and Prevention, Hyogo College of Medicine, Nishinomiya, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakajima
authors.affiliation: Department of Infection Control and Prevention, Hyogo College of Medicine, Nishinomiya, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Takesue
authors.affiliation: Department of Microbiology, Hyogo College of Medicine, Nishinomiya, Japan. Electronic address: tmokuno@hyo-med.ac.jp.
authors.forename: Toshiomi
authors.initials: T
authors.lastname: Okuno
mesh_terms: D000293:Adolescent; D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects*; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009107:Mumps / Q000097:blood / Q000276:immunology*; D009108:Mumps Vaccine / Q000008:administration & dosage* / Q000276:immunology; D009109:Mumps virus / Q000276:immunology*
chemical_list: C573415:IFNG protein, human; D009108:Mumps Vaccine; D007371:Interferon-gamma
keywords: Cell-mediated immunity; Interferon-γ; Mumps vaccine; Mumps virus; Serum antibody