title: [Effects of thymosin alpha1 on immune effector molecules of mouse].
abstract: OBJECTIVE
  To analysis the effects of Talpha1 on the immune effector molecules in mouse immune system.
  
  
  METHODS
  Sixty five BABL/c mice were divided into four groups: CsA group (n=20), Talpha1 group (n= 20), CsA+Talpha1 group (n=20) and control group (n=5). In the 3 experimental groups, 10 mg/kg CsA, 400 microg/ kg Talpha1, 10 mg/kg CsA+400 microg/kg Talpha1 were respectively administrated by intraperitoneal injection daily. Luminex was performed for cytokine detection at 1 d, 7 d, 14 d, 21 d day after the above treatments. Lymphocyte culture was prepared with the mouse spleen suspension, and then treated with 0. 25 mg/mL CsA, 10 microg/mL Talpha1 or 0.25 mg/mL CsA+10 microg/mL Talpha1 in vitro, respectively. Three days later, OD values of each treated lymphocyte culture and several cytokines in the culture were measured.
  
  
  RESULTS
  Compared with other groups, CsA+Talpha1 group had significant lower IL-1alpha, IL-2, IL-6, IL-17, and significant higher IL-10 at 1 d, 7 d, 14 d, 21 d after the treatments (P < 0.05). Three days after the culture, OD value in the control group was significantly higher than that in Talppha1 group, CsA group, and CsA+ Talpha1 group (P < 0.05). IL-1alpa and IL-6 in the control group were significantly higher than those in the experiment groups (P < 0.05), while IL-10 in the control group was significantly lower than that in the experiment groups (P < 0.05). IL-2 and IL-17 were similar.
  
  
  CONCLUSIONS
  Talpha1 show regulatory effect on the immune effector molecules which could promote Th1 cells transforming to Th2 cells.
authors.forename: Wei-ye
authors.initials: WY
authors.lastname: Li
authors.forename: Hui-min
authors.initials: HM
authors.lastname: Lu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Hu
authors.forename: Zhao-da
authors.initials: ZD
authors.lastname: Zhang
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018417:Th1 Cells / Q000166:cytology*; D018418:Th2 Cells / Q000166:cytology*; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D016207:Cytokines; D013947:Thymosin; D000077596:Thymalfasin