title: Multidrug-resistant genes of aminoglycoside-modifying enzymes and 16S rRNA methylases in Acinetobacter baumannii strains.
abstract: We examined the distribution of genes of aminoglycoside-modifying enzymes and 16S rRNA methylases in multidrug-resistant Acinetobacter baumannii to explore the association of these genes with drug resistance. Strains isolated from clinical specimens were screened using an automatic microbial identification system, and 9 aminoglycoside-modifying enzyme and 6 16S rRNA methylase genes were analyzed using polymerase chain reaction and verified by DNA sequencing. Next, sequence alignment was carried out using the Chromas software and a susceptibility test was performed using the Kirby-Bauer disk diffusion method. Genes encoding aminoglycoside-modifying enzymes were detected in all 20 strains of multidrug-resistant A. baumannii. The positive rates of aac(3')-I, aac(6')-Ib, ant(3'')-I, and aph(3')-I were 90.0, 90.0, 85.0, and 35.0%, respectively. However, genes encoding 16S rRNA methylases were not positively detected in the 20 strains of multidrug-resistant A. baumannii. The resistance of multidrug-resistant A. baumannii may be associated with aminoglycoside-modifying enzyme genes but not with 16S rRNA methylase genes.
authors.affiliation: Department of Clinical Laboratory, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu Province, China.
authors.forename: J-T
authors.initials: JT
authors.lastname: Wen
authors.affiliation: Department of Clinical Laboratory, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu Province, China yanzhoucn@126.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Clinical Laboratory, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu Province, China.
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Clinical Laboratory, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu Province, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D000617:Aminoglycosides / Q000008:administration & dosage / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D008780:Methyltransferases / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase; D000123:Acetyltransferases; C045539:aminoglycoside N(3')-acetyltransferase