title: Cell Wall Polysaccharides of Candida albicans Induce Mast Cell Degranulation in the Gut.
abstract: We investigated Candida albicans-induced mast cell degranulation in vitro and in vivo. Cell wall fraction but not culture supernatant and cell membrane fraction prepared from hyphally grown C. albicans induced β-hexosaminidase release in RBL-2H3 cells. Cell wall mannan and soluble β-glucan fractions also induced β-hexosaminidase release. Histological examination of mouse forestomach showed that C. albicans gut colonization induces mast cell degranulation. However, intragastric administration of cell wall fraction failed to induce mast cell degranulation. We propose that cell wall polysaccharides are responsible for mast cell degranulation in the C. albicans-colonized gut.
authors.affiliation: Graduate School of Life Science, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Sakurai
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan ; Present address: Faculty of Medicine, Shimane University, Izumo, Shimane 693-8501, Japan.
authors.forename: Natsu
authors.initials: N
authors.lastname: Yamaguchi
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Sonoyama
keywords: Candida albicans; degranulation; mannan; mast cell; β-glucan