title: Microfluidics-assisted engineering of polymeric microcapsules with high encapsulation efficiency for protein drug delivery.
abstract: In this study, microfluidic technology was employed to develop protein formulations. The microcapsules were produced with a biphasic flow to create water-oil-water (W/O/W) double emulsion droplets with ultrathin shells. Optimized microcapsule formulations containing 1% (w/w) bovine serum albumin (BSA) in the inner phase were prepared with poly(vinyl alcohol), polycaprolactone and polyethylene glycol. All the particles were found to be intact and with a particle size of 23-47 μm. Furthermore, the particles were monodisperse, non-porous and stable up to 4 weeks. The encapsulation efficiency of BSA in the microcapsules was 84%. The microcapsules released 30% of their content within 168 h. This study demonstrates that microfluidics is a powerful technique for engineering formulations for therapeutic proteins.
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland; School of Engineering and Applied Science, Department of Physics, Harvard University, Cambridge, MA 02138, USA. Electronic address: jenni.pessi@helsinki.fi.
authors.forename: Jenni
authors.initials: J
authors.lastname: Pessi
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland.
authors.forename: Hélder A
authors.initials: HA
authors.lastname: Santos
authors.affiliation: School of Engineering and Applied Science, Department of Physics, Harvard University, Cambridge, MA 02138, USA; LECOM School of Pharmacy, Erie, PA 16509, USA.
authors.forename: Inna
authors.initials: I
authors.lastname: Miroshnyk
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland.
authors.lastname: JoukoYliruusi
authors.affiliation: School of Engineering and Applied Science, Department of Physics, Harvard University, Cambridge, MA 02138, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Weitz
authors.affiliation: School of Engineering and Applied Science, Department of Physics, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Sabiruddin
authors.initials: S
authors.lastname: Mirza
mesh_terms: D002214:Capsules; D004339:Drug Compounding / Q000379:methods*; D016503:Drug Delivery Systems; D004655:Emulsions; D044085:Microfluidics; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility
chemical_list: D002214:Capsules; D004655:Emulsions; D011108:Polymers; D012710:Serum Albumin, Bovine
keywords: Drug delivery; Ethyl acetate (PubChem CID 8857); Microcapsules; Microfluidics; Poly(vinyl alcohol) (PubChem CID 11199); Polycaprolactone; Polycaprolactone (PubChem CID 10401); Polyethylene glycol (PubChem CID 174); Therapeutic proteins