title: Downregulation of lipogenesis and fatty acid oxidation in the subcutaneous adipose tissue of morbidly obese women.
abstract: OBJECTIVE
  The aim of this study was to analyse the expression of crucial genes in fatty acid metabolism in visceral (VAT) and subcutaneous (SAT) adipose tissue samples from morbidly obese women.
  
  
  METHODS
  The VAT and SAT expression of key genes in 145 morbidly obese women (MO, BMI > 40 Kg/m(2) ) and 18 normal weight control women by RT-PCR and Western Blot was analyzed.
  
  
  RESULTS
  In SAT, the expression levels of the genes related to lipogenesis and fatty acid oxidation were significantly lower in MO than in controls. In VAT, most of the lipogenic genes studied had similar expression levels in MO and control cohort. Regarding inflammation, IL6 was significantly higher in MO in both tissues whereas TNFα mRNA expression was significantly higher only in VAT.
  
  
  CONCLUSIONS
  Our results indicate that in morbidly obese patients, lipogenesis and fatty acid oxidation are downregulated in SAT, whereas in VAT these pathways are almost unchanged. By contrast, inflammation is induced in both adipose tissues. It is hypothesized that, in this type of extreme obesity, SAT works to limit any further development of fat mass, decreasing the expression of lipogenic and FA oxidative genes whereas VAT depot might have lost this capability.
authors.affiliation: Grup GEMMAIR (AGAUR), Grup de Recerca en Medicina Aplicada Hospital Joan XXIII, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació de la Salut Pere Virgili (IISPV), Mallafré Guasch, 4, 43007, Tarragona, Spain; Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007, Tarragona, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Auguet
authors.forename: Esther
authors.initials: E
authors.lastname: Guiu-Jurado
authors.forename: Alba
authors.initials: A
authors.lastname: Berlanga
authors.forename: Ximena
authors.initials: X
authors.lastname: Terra
authors.forename: Salomé
authors.initials: S
authors.lastname: Martinez
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Porras
authors.forename: Andra
authors.initials: A
authors.lastname: Ceausu
authors.forename: Fátima
authors.initials: F
authors.lastname: Sabench
authors.forename: Mercé
authors.initials: M
authors.lastname: Hernandez
authors.forename: Carmen
authors.initials: C
authors.lastname: Aguilar
authors.forename: Joan Josep
authors.initials: JJ
authors.lastname: Sirvent
authors.forename: Daniel
authors.initials: D
authors.lastname: Del Castillo
authors.forename: Cristóbal
authors.initials: C
authors.lastname: Richart
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000328:Adult; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics*; D050155:Lipogenesis / Q000235:genetics*; D008875:Middle Aged; D009767:Obesity, Morbid / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D010084:Oxidation-Reduction; D050151:Subcutaneous Fat / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D014409:Tumor Necrosis Factor-alpha