title: Development and application of a quantitative fluorescence-based immunochromatographic assay for fumonisin b1 in maize.
abstract: A fluorescence-based immunochromatographic assay (ICA) for fumonisin B1 (FB1) that employs conjugates of fluorescent microspheres and monoclonal antibodies (FM-mAbs) as detection reporters is described. The ICA is based on the competitive reaction between FB1-bovine serum albumin (BSA; test line) and the target FB1 for binding to the FM-mAb conjugates. A limit of detection (LOD) for FB1 of 0.12 ng/mL was obtained, with an analytical working range of 0.25-2.0 ng/mL (corresponding to 250-2000 μg/kg in maize flour samples, according to the extraction procedure). The recoveries of the ICA to detect FB1 in maize samples ranged from 91.4 to 118.2%. A quantitative comparison of the fluorescence-based ICA and HPLC-MS/MS analysis of naturally contaminated maize samples indicated good agreement between the two methods (r(2) = 0.93). By replacing the target of interest, the FM-based ICA can easily be extended to other chemical contaminants and thus represents a versatile strategy for food safety analysis.
authors.affiliation: College of Veterinary Medicine, China Agricultural University , Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China.
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Chenglong
authors.initials: C
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Yu
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
authors.forename: Sarah
authors.initials: S
authors.lastname: De Saeger
mesh_terms: D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D037341:Fumonisins / Q000032:analysis*; D057230:Limit of Detection; D009183:Mycotoxins / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1