title: Gellan sulfate core platinum coil with tenascin-C promotes intra-aneurysmal organization in rats.
abstract: The aims of this study were to develop a new coil, gellan sulfate core platinum coil (GSCC), that delivers tenascin-C (TNC) to an aneurysm (GSCC-TNC) and to evaluate the effects on intra-aneurysmal organization. We performed in vitro adsorption tests of TNC to gellan sulfate (GS). GSCC-TNC was produced by immersing GSCC in TNC solution under the following conditions (TNC concentration 10, 50, or 100 μg/mL; TNC immersion time 15, 30, or 60 min) by monitoring intra-aneurysmal organization in a rat blind-ended aneurysm model. In addition, 20 rats randomly underwent implantation of a platinum coil or the GSCC-TNC produced under optimum conditions into an aneurysm, whose organization effects were compared in a blind fashion at 2 weeks post-surgery. GS demonstrated a high affinity to TNC in a dose-dependent fashion (affinity constant = 1.79 × 10(10) (M(-1))). GSCC immersed in 10 μg/mL of TNC solution for 30 and 60 min induced similar and better organization of aneurysmal cavity compared with that for 15 min (the ratio of the organized areas in an aneurysmal cavity-15 min, 27.2 ± 11.8 %; 30 min, 75.6 ± 11.9 %; 60 min, 82.6 ± 19.7 %, respectively) with the preservation of the aneurysmal wall structure, while higher TNC concentrations caused the destruction of the aneurysmal wall. GSCC-TNC produced under 10 μg/mL of TNC solution for 30 min showed a significantly better organization of aneurysms compared with bare platinum coils in rats. A newly developed coil, GSCC-TNC, may be effective for improving intra-aneurysmal organization after coil embolization.
authors.affiliation: Department of Neurosurgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Hamada
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Miura
authors.forename: Naoki
authors.initials: N
authors.lastname: Toma
authors.forename: Keiichi
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Kyoko
authors.initials: K
authors.lastname: Imanaka-Yoshida
authors.forename: Satoshi
authors.initials: S
authors.lastname: Matsushima
authors.forename: Toshimichi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Waro
authors.initials: W
authors.lastname: Taki
authors.forename: Hidenori
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004621:Embolization, Therapeutic / Q000295:instrumentation*; D002532:Intracranial Aneurysm / Q000473:pathology / Q000628:therapy*; D008297:Male; D010984:Platinum; D011134:Polysaccharides; D051381:Rats; D017207:Rats, Sprague-Dawley; D013463:Sulfuric Acid Esters; D019063:Tenascin / Q000008:administration & dosage* / Q000493:pharmacokinetics
chemical_list: D011134:Polysaccharides; D013463:Sulfuric Acid Esters; D019063:Tenascin; C470030:gellan sulfate; D010984:Platinum