title: miR-96/HBP1/Wnt/β-catenin regulatory circuitry promotes glioma growth.
abstract: We found that miR-96 is overexpressed in glioma, and its level inversely correlates with the survival of patients. The reduction in miR-96 abundance suppresses the proliferation and colony formation of glioma cells. The tumorigenicity of U-87 MG cells is reduced by miR-96 silencing. miR-96 contributes to the activation of Wnt/β-catenin pathway in glioma cells. HMG-box transcription factor 1 (HBP-1), a Wnt/β-catenin pathway inhibitor, is suppressed by miR-96. The reactivation of Wnt/β-catenin signaling causes an increase in the proliferation of glioma cells, and a decrease in miR-96 expression. On the other hand, HBP1 silencing promotes miR-96 expression. Collectively, miR-96 contributes to the progression of glioma by enhancing the activation of the Wnt/β-catenin pathway, and the miR-96/HBP1/Wnt/β-catenin regulatory circuitry promotes the proliferation of glioma cells.
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Yingbing
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Qi
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China. Electronic address: qyfycss@gmail.com.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Che
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D016019:Survival Analysis; D051153:Wnt Proteins / Q000378:metabolism*; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C541492:MIRN96 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D051153:Wnt Proteins; D051176:beta Catenin; C062189:histone DNA binding protein-1
keywords: Glioma; HMG-box transcription factor 1; Proliferation; Wnt/β-catenin; miR-96