title: Metabolite of tryptophan promoting changes in EEG signal and the oxidative status of the brain.
abstract: Tryptophan is an essential amino acid precursor of neurotransmitter serotonin and triptamine. During its metabolism, indole-3-acetic acid (IAA) is generated; this substance presents both antioxidant and prooxidant effects in different biological systems in addition to hipoglicemic effects. To date, electroencephalography (EEG) has been used to evaluate the temporal effect of several substances in neurotransmission. The goal of this study was to characterize the effect of IAA in the brain by analysing the EEG signal and evaluate the oxidative status by means of biochemical parameters. The EEG was acquired by using a noninvasive method, and the brain electric signal was analysed by advanced digital signal processing techniques to determinate the energy signal filtered in different band frequencies. Furthermore, the oxidative status of the brain was investigated by measuring the activity of antioxidant enzymes and lipid peroxidation as well as blood glucose rates of the animals treated with different doses of IAA. Our results showed the relationship of IAA administration with changes in EEG signals. The oxidative status of the brain was modified by IAA after 14 days of treatment.
authors.affiliation: Department of Biological Science, State University of Santa Cruz (UESC), Ilhéus, Bahia, Brazil; Laboratory of Biological Chemistry, Department of Basic Sciences, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo (USP), Pirassununga, São Paulo, Brazil.
authors.forename: Rosana
authors.initials: R
authors.lastname: Ferrari
authors.forename: Silvana Maria Picolli
authors.initials: SM
authors.lastname: Pugini
authors.forename: Aldo Ivan Cespedes
authors.initials: AI
authors.lastname: Arce
authors.forename: Ernane Jose Xavier
authors.initials: EJ
authors.lastname: Costa
authors.forename: Mariza Pires
authors.initials: MP
authors.lastname: de Melo
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004569:Electroencephalography; D005980:Glutathione Reductase / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D007210:Indoleacetic Acids; D008315:Malondialdehyde; C030737:indoleacetic acid; D014364:Tryptophan; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase
keywords: IAA; SOD; brain electrical activity; neurotransmission; oxidative stress