title: Trichoderma atroviride transcriptional regulator Xyr1 supports the induction of systemic resistance in plants.
abstract: As a result of a transcriptome-wide analysis of the ascomycete Trichoderma atroviride, mycoparasitism-related genes were identified; of these, 13 genes were further investigated for differential expression. In silico analysis of the upstream regulatory regions of these genes pointed to xylanase regulator 1 (Xyr1) as a putatively involved regulatory protein. Transcript analysis of the xyr1 gene of T. atroviride in confrontation with other fungi allowed us to determine that xyr1 levels increased during mycoparasitism. To gain knowledge about the precise role of Xyr1 in the mycoparasitic process, the corresponding gene was deleted from the T. atroviride genome. This resulted in strong reductions in the transcript levels of axe1 and swo1, which encode accessory cell wall-degrading enzymes considered relevant for mycoparasitism. We also analyzed the role of Xyr1 in the Trichoderma-Arabidopsis interaction, finding that the plant response elicited by T. atroviride is delayed if Xyr1 is missing in the fungus.
authors.affiliation: Department for Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria.
authors.forename: Barbara
authors.initials: B
authors.lastname: Reithner
authors.affiliation: Department for Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria.
authors.forename: Astrid R
authors.initials: AR
authors.lastname: Mach-Aigner
authors.affiliation: Research Area Fungal Development and Gene Expression, Laboratorio Nacional de Genómica para la Biodiversidad, Cinvestav Campus Guanajuato, Irapuato, Guanajuato, Mexico.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Herrera-Estrella
authors.affiliation: Department for Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria robert.mach@tuwien.ac.at.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Mach
mesh_terms: D017360:Arabidopsis / Q000276:immunology* / Q000382:microbiology*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*
chemical_list: D014157:Transcription Factors