title: Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy.
abstract: The actin cytoskeleton plays a fundamental role in controlling several steps during regulated exocytosis. Here, we describe a combination of procedures that are aimed at studying the dynamics and the mechanism of the actin cytoskeleton in the salivary glands of live rodents, a model for exocrine secretion. Our approach relies on intravital microscopy, an imaging technique that enables imaging biological events in live animals at a subcellular resolution, and it is complemented by the use of pharmacological agents and indirect immunofluorescence in the salivary tissue.
authors.affiliation: Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Dr. 303A, 20892-4340, Bethesda, MD, USA.
authors.forename: Oleg
authors.initials: O
authors.lastname: Milberg
authors.forename: Muhibullah
authors.initials: M
authors.lastname: Tora
authors.forename: Akiko
authors.initials: A
authors.lastname: Shitara
authors.forename: Taishin
authors.initials: T
authors.lastname: Takuma
authors.forename: Andrius
authors.initials: A
authors.lastname: Masedunskas
authors.forename: Roberto
authors.initials: R
authors.lastname: Weigert
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D005260:Female; D008297:Male; D051379:Mice; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence; D012469:Salivary Glands / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D022142:Secretory Vesicles / Q000378:metabolism
chemical_list: D000199:Actins