title: Rap2B promotes migration and invasion of human suprarenal epithelioma.
abstract: The aim of our study was to elucidate the role of Rap2B in the development of human suprarenal epithelioma and to investigate the effect of Rap2B on suprarenal epithelioma cells migration and invasion. We use tissue microarray and immunohistochemistry to evaluate Rap2B staining in 75 suprarenal epithelioma tissues and 75 tumor-adjacent normal renal tissues. And the expression of Rap2B protein in human suprarenal epithelioma cells and tissues was detected by western blot simultaneously. The role of Rap2B in suprarenal epithelioma cells migration and invasion was detected by using wound healing assay, cell migration assay, and matrigel invasion assay. After that, we performed western blot analysis and gelatin zymography to detect MMP-2 protein expression and enzyme activity. Our research showed that Rap2B expression was increased in tumor tissues compared with tumor-adjacent normal renal tissues. But no correlation was found between Rap2B expression and clinicopathological parameters. In addition, we found that Rap2B promoted the cell migration and invasion abilities, and Rap2B increased MMP-2 expression and enzyme activity in suprarenal epithelioma cells. Our data indicated that Rap2B expression is significantly increased in human suprarenal epithelioma and Rap2B can promote the cell migration and invasion abilities, which may provide a new target for the treatment of suprarenal epithelioma.
authors.affiliation: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002, Jiangsu, People's Republic of China.
authors.forename: Jie-Hui
authors.initials: JH
authors.lastname: Di
authors.forename: De-Bao
authors.initials: DB
authors.lastname: Qu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Lu
authors.forename: Lian-Tao
authors.initials: LT
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Cheng
authors.forename: Yong
authors.initials: Y
authors.lastname: Xin
authors.forename: Long-Zhen
authors.initials: LZ
authors.lastname: Zhang
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jun-Nian
authors.initials: JN
authors.lastname: Zheng
mesh_terms: D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D046888:Tissue Array Analysis; D020668:rap GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020778:Matrix Metalloproteinase 2; C065562:RAP2B protein, human; D020668:rap GTP-Binding Proteins