title: [Expression of CD133 in primary retroperitoneal leiomyosarcoma and its relationship with Ki-67].
abstract: OBJECTIVE
  To explore the correlation between CD133 and proliferative marker Ki-67 in primary retroperitoneal leiomyosarcoma and evaluate their prognostic values.
  
  
  METHODS
  A total of 50 primary retroperitoneal leiomyosarcoma samples were collected at our hospital from January 2000 to December 2012. There were 9 males and 41 females. All samples were analyzed for CD133 and Ki-67 protein expression by PV6000 immunohistochemistry. Their median age was 49 (27-75) years. All cases were successfully followed up.
  
  
  RESULTS
  CD133 expression was detected in 74.00% (37/50) of primary retroperitoneal leiomyosarcoma samples. The CD133-positive rates of histological grades I & II primary leiomyosarcoma were 7/13 and 76.70% (23/30) respectively. All grade III samples (n = 7) were CD133 positive. And the expression of CD133 had a positive correlation with tumor size, mitotic counts and histological grade (χ(2) = 4.925, 4.525, 10.080; P = 0.026, 0.033, 0.013). The survival time of CD133-positive patients with M (Q1, Q3) was 32 (17, 56) months versus 44 (26, 65) months for those negative ones. The expression of Ki-67 was detected in 84.00% (42/50) of primary retroperitoneal leiomyosarcoma samples and its expression showed a positive correlation with mitotic counts. The expressions of CD133 and Ki-67 were positively correlated in primary retroperitoneal leiomyosarcomas (P = 0.009). Log-rank test showed that positive expressions of CD133 and Ki-67 and 5-year patient survival rate were correlated (P = 0.021, 0.049). Multivariate analysis showed that CD133 and mitotic counts were independent prognostic indicators for primary retroperitoneal leiomyosarcomas (HR = 2.040, 2.422; P = 0.000, 0.018).
  
  
  CONCLUSIONS
  CD133 plays an important role in the progression of primary retroperitoneal leiomyosarcomas so that it may be used as a marker for patient prognosis. Combined detection of CD133 and Ki-67 has a prognostic value in patients with primary retroperitoneal leiomyosarcoma.
authors.affiliation: Departments of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yaoguang
authors.initials: Y
authors.lastname: She
authors.affiliation: Departments of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Liang
authors.affiliation: Departments of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Departments of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Departments of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Huang
authors.affiliation: Departments of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Departments of General Surgery, Chinese PLA General Hospital, Beijing 100853, China. Email: peiyuli301@163.com.
authors.forename: Peiyu
authors.initials: P
authors.lastname: Li
mesh_terms: D000071916:AC133 Antigen; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D007890:Leiomyosarcoma / Q000175:diagnosis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism*; D011379:Prognosis; D012186:Retroperitoneal Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D012189:Retrospective Studies
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D019394:Ki-67 Antigen; C000606023:PROM1 protein, human; D010455:Peptides