title: Liver cancer cells: targeting and prolonged-release drug carriers consisting of mesoporous silica nanoparticles and alginate microspheres.
abstract: A new microsphere consisting of inorganic mesoporous silica nanoparticles (MSNs) and organic alginate (denoted as MSN@Alg) was successfully synthesized by air-dynamic atomization and applied to the intracellular drug delivery systems (DDS) of liver cancer cells with sustained release and specific targeting properties. MSN@Alg microspheres have the advantages of MSN and alginate, where MSN provides a large surface area for high drug loading and alginate provides excellent biocompatibility and COOH functionality for specific targeting. Rhodamine 6G was used as a model drug, and the sustained release behavior of the rhodamine 6G-loaded MSN@Alg microspheres can be prolonged up to 20 days. For targeting therapy, the anticancer drug doxorubicin was loaded into MSN@Alg microspheres, and the (lysine)4-tyrosine-arginine-glycine-aspartic acid (K4YRGD) peptide was functionalized onto the surface of MSN@Alg for targeting liver cancer cells, hepatocellular carcinoma (HepG2). The results of the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and confocal laser scanning microscopy indicate that the MSN@Alg microspheres were successfully uptaken by HepG2 without apparent cytotoxicity. In addition, the intracellular drug delivery efficiency was greatly enhanced (ie, 3.5-fold) for the arginine-glycine-aspartic acid (RGD)-labeled, doxorubicin-loaded MSN@Alg drug delivery system compared with the non-RGD case. The synthesized MSN@Alg microspheres show great potential as drug vehicles with high biocompatibility, sustained release, and targeting features for future intracellular DDS.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Yu-Te
authors.initials: YT
authors.lastname: Liao
authors.affiliation: Department of Urology, Taipei Medical University-Shuang Ho Hospital, New Taipei City, Taiwan.
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Liu
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Jiashing
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan ; Division of Medical Engineering Research, National Health Research Institutes, Zhunan Township, Miaoli County, Taiwan.
authors.forename: Kevin C-W
authors.initials: KC
authors.lastname: Wu
mesh_terms: D000464:Alginates / Q000008:administration & dosage / Q000737:chemistry*; D000818:Animals; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000737:chemistry; D004058:Diffusion; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000737:chemistry; D056945:Hep G2 Cells; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D058990:Molecular Targeted Therapy / Q000379:methods*; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry*; D009842:Oligopeptides / Q000008:administration & dosage / Q000493:pharmacokinetics; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D016896:Treatment Outcome
chemical_list: D000464:Alginates; D003692:Delayed-Action Preparations; D006603:Hexuronic Acids; D053769:Nanocapsules; D009842:Oligopeptides; D012822:Silicon Dioxide; C047981:arginyl-glycyl-aspartic acid; D004317:Doxorubicin; D020723:Glucuronic Acid
keywords: alginate; atomization; mesoporous silica nanoparticles; sustained release; targeting therapy