title: Metabolomic analysis of amino acid and energy metabolism in rats supplemented with chlorogenic acid.
abstract: This study was conducted to investigate effects of chlorogenic acid (CGA) supplementation on serum and hepatic metabolomes in rats. Rats received daily intragastric administration of either CGA (60 mg/kg body weight) or distilled water (control) for 4 weeks. Growth performance, serum biochemical profiles, and hepatic morphology were measured. Additionally, serum and liver tissue extracts were analyzed for metabolomes by high-resolution (1)H nuclear magnetic resonance-based metabolomics and multivariate statistics. CGA did not affect rat growth performance, serum biochemical profiles, or hepatic morphology. However, supplementation with CGA decreased serum concentrations of lactate, pyruvate, succinate, citrate, β-hydroxybutyrate and acetoacetate, while increasing serum concentrations of glycine and hepatic concentrations of glutathione. These results suggest that CGA supplementation results in perturbation of energy and amino acid metabolism in rats. We suggest that glycine and glutathione in serum may be useful biomarkers for biological properties of CGA on nitrogen metabolism in vivo.
authors.affiliation: State Key Laboratory of Food Science and Technology, College of Life Science and Food Engineering, Nanchang University, Rm325, 235 Nanjing Street, Nanchang, 330047, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ruan
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Wen
authors.forename: Sheng
authors.initials: S
authors.lastname: Ding
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.forename: Peng
authors.initials: P
authors.lastname: Liao
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Deng
authors.forename: Houssein
authors.initials: H
authors.lastname: Assaad
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002726:Chlorogenic Acid / Q000494:pharmacology*; D019587:Dietary Supplements; D004734:Energy Metabolism / Q000187:drug effects*; D005260:Female; D055432:Metabolomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D000596:Amino Acids; D002726:Chlorogenic Acid