title: [Analysis of protein using mass spectrometry].
abstract: Recently, mass spectrometry has become a powerful tool in cancer research. Mass spectrometry represents the method that allows identification, quantification and characterization of proteins in bio-logical samples. Nowadays, it is mainly used for bio-marker discovery that can enable early detection of cancer. This article is focused on protein analysis by mass spectrometry. At first, mass spectrometry and its importance in proteomics are described. Subsequently ionization type and mass analyzers are discussed. This relates to the possibility of online or offline analysis connection with separation techniques, such as liquid chromatography and electrophoresis. Different approaches for preparing proteins and methods of analysis of bio-molecules using mass spectrometers are described. In addition, the possibility of mass spectrometric analyses of samples and data processing are discussed.
authors.forename: P
authors.initials: P
authors.lastname: Dvořáková
authors.forename: L
authors.initials: L
authors.lastname: Hernychova
authors.forename: B
authors.initials: B
authors.lastname: Vojtěšek
mesh_terms: D002853:Chromatography, Liquid; D004586:Electrophoresis; D006801:Humans; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000032:analysis; D009369:Neoplasms / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D040901:Proteomics
chemical_list: D009363:Neoplasm Proteins; D011506:Proteins