title: Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain.
abstract: The individual contribution of different progenitor subtypes towards the mature rodent cerebral cortex is not fully understood. Intermediate progenitor cells (IPCs) are key to understanding the regulation of neuronal number during cortical development and evolution, yet their exact contribution is much debated. Intermediate progenitors in the cortical subventricular zone are defined by expression of T-box brain-2 (Tbr2). In this study we demonstrate by using the Tbr2(Cre) mouse line and state-of-the-art cell lineage labeling techniques, that IPC derived cells contribute substantial proportions 67.5% of glutamatergic but not GABAergic or astrocytic cells to all cortical layers including the earliest generated subplate zone. We also describe the laminar dispersion of clonally derived cells from IPCs using a recently described clonal analysis tool (CLoNe) and show that pair-generated cells in different layers cluster closer (142.1 ± 76.8 μm) than unrelated cells (294.9 ± 105.4 μm). The clonal dispersion from individual Tbr2 positive intermediate progenitors contributes to increasing the cortical surface. Our study also describes extracortical contributions from Tbr2+ progenitors to the lateral olfactory tract and ventromedial hypothalamic nucleus.
authors.affiliation: Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
authors.forename: Navneet A
authors.initials: NA
authors.lastname: Vasistha
authors.affiliation: Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
authors.forename: Fernando
authors.initials: F
authors.lastname: García-Moreno
authors.affiliation: Oxford Centre for Industrial and Applied Mathematics (OCIAM), University of Oxford, 24-29 St Giles', Oxford, UK.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Arora
authors.affiliation: Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
authors.forename: Amanda F P
authors.initials: AF
authors.lastname: Cheung
authors.affiliation: Renal Department, University Medical Centre, Centre for Clinical Research, Breisacher Strasse 66, 79106 Freiburg, Germany BIOSS Centre of Biological Signalling Studies, Albert-Ludwigs-University, Freiburg, Germany.
authors.forename: Sebastian J
authors.initials: SJ
authors.lastname: Arnold
authors.affiliation: Sir William Dunn School of Pathology, Oxford, UK.
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Robertson
authors.affiliation: Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Molnár
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000502:physiology; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002540:Cerebral Cortex / Q000196:embryology* / Q000378:metabolism; D059330:GABAergic Neurons / Q000378:metabolism / Q000502:physiology; D020547:Lateral Ventricles / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D058953:Neural Stem Cells / Q000378:metabolism / Q000502:physiology*; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C491198:Eomes protein, mouse; D020825:T-Box Domain Proteins
keywords: CLoNe; Cerebral cortex; clonal analysis; fate-mapping; intermediate progenitors; subventricular zone