title: Pick1 modulates ephrinB1-induced junctional disassembly through an association with ephrinB1.
abstract: Members of the Eph family have been implicated in the formation of cell-cell boundaries, cell movement, and positioning during development in the context of cancer progression. De-regulation of this signaling system is linked to the promotion of more aggressive and metastatic tumor phenotypes in a large variety of human cancers, including breast, lung, and prostate cancer, melanoma, and leukemia. Thus, it is interesting to consider the case of cancer progression where de-regulation of the Eph/ephrin signaling system results in invasion and metastasis. Here, we present evidence that Pick1, one of the essential components of the adherens junction, recovers ephrinB1-induced cell-cell de-adhesion. Loss of Pick1 leads to dissociation of epithelial cells via disruption of the adherens junction, a phenotype similar to ephrinB1 overexpression. In addition, overexpressed ephrinB1-induced disruption of the adherens junction is rescued via binding to Pick1. These data indicate that Pick1 is involved in regulating the cell-cell junction in epithelial cells, and this may influence therapeutic strategy decisions with regards to cell adhesion molecules in metastatic disease.
authors.affiliation: ABRC, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Jungeun
authors.initials: J
authors.lastname: Son
authors.affiliation: Aquaculture Management Division, National Fisheries Research and Development Institute, Busan 619-705, South Korea.
authors.forename: Mi Seon
authors.initials: MS
authors.lastname: Park
authors.affiliation: ABRC, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Inji
authors.initials: I
authors.lastname: Park
authors.affiliation: ABRC, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Hyun-Kyung
authors.initials: HK
authors.lastname: Lee
authors.affiliation: ABRC, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Soo-Ho
authors.initials: SH
authors.lastname: Lee
authors.affiliation: ABRC, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Byungwoo
authors.initials: B
authors.lastname: Kang
authors.affiliation: Aquaculture Management Division, National Fisheries Research and Development Institute, Busan 619-705, South Korea.
authors.forename: Byung-Hwa
authors.initials: BH
authors.lastname: Min
authors.affiliation: ABRC, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Jaewoong
authors.initials: J
authors.lastname: Ryoo
authors.affiliation: ABRC, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Sanggyu
authors.initials: S
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Jong-Sup
authors.initials: JS
authors.lastname: Bae
authors.affiliation: Department of Pharmacology, College of Medicine, Kyungpook National University, Daegu 700-422, South Korea.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Anatomy, College of Medicine, Kyungpook National University, Daegu 700-422, South Korea.
authors.forename: Mae-Ja
authors.initials: MJ
authors.lastname: Park
authors.affiliation: ABRC, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, South Korea. Electronic address: leeh@knu.ac.kr.
authors.forename: Hyun-Shik
authors.initials: HS
authors.lastname: Lee
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002450:Cell Communication / Q000502:physiology*; D002478:Cells, Cultured; D047108:Embryonic Development / Q000502:physiology*; D036387:Ephrin-B1 / Q000378:metabolism*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D011485:Protein Binding; D014982:Xenopus laevis
chemical_list: D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D036387:Ephrin-B1; D009687:Nuclear Proteins; C498297:PICk1 protein, human
keywords: Cell–cell junctions; Ephrinb1; Metastatic diseases; Pick1; Xenopus