title: Methyl jasmonate affects phenolic metabolism and gene expression in blueberry (Vaccinium corymbosum).
abstract: Blueberry (Vaccinium corymbosum) is a fruit very much appreciated by consumers for its antioxidant potential and health-promoting traits. Its beneficial potential properties are mainly due to a high content of anthocyanins and their amount can change after elicitation with methyl jasmonate. The aim of this work is to evaluate the changes in expression of several genes, accumulation of phenolic compounds and alterations in antioxidant potential in two different blueberry cultivars ('Duke' and 'Blueray') in response to methyl jasmonate (0.1 mM). Results showed that 9 h after treatment, the expression of phenylalanine ammonium lyase, chalcone synthase and anthocyanidin synthase genes was stimulated more in the 'Blueray' variety. Among the phenols measured an increase was recorded also for epicatechin and anthocyanin concentrations. 'Duke' is a richer sourche of anthocyanins compared to 'Blueray', treatment with methyl jasmonate promoted in 'Blueray' an increase in pigments as well as in the antioxidant potential, especially in fully ripe berries, but treated 'Duke' berries had greater levels, which were not induced by methyl jasmonate treatment. In conclusion, methyl jasmonate was, in some cases, an effective elicitor of phenolic metabolism and gene expression in blueberry, though with different intensity between cultivars.
authors.affiliation: Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, Università degli Studi di Milano, Via Celoria 2, 20133, Milan, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Cocetta
authors.forename: Mara
authors.initials: M
authors.lastname: Rossoni
authors.forename: Claudio
authors.initials: C
authors.lastname: Gardana
authors.forename: Ilaria
authors.initials: I
authors.lastname: Mignani
authors.forename: Antonio
authors.initials: A
authors.lastname: Ferrante
authors.forename: Anna
authors.initials: A
authors.lastname: Spinardi
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000872:Anthocyanins / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism; D029798:Blueberry Plants / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis; D003517:Cyclopentanes / Q000494:pharmacology*; D044948:Flavonols / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D054883:Oxylipins / Q000494:pharmacology*; D010636:Phenols / Q000378:metabolism*; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D020005:Propanols / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013267:Stilbenes / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
chemical_list: D000085:Acetates; D000872:Anthocyanins; D001713:Biphenyl Compounds; D002241:Carbohydrates; D003517:Cyclopentanes; D044948:Flavonols; D016166:Free Radical Scavengers; D054883:Oxylipins; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D020005:Propanols; D012333:RNA, Messenger; D013267:Stilbenes; C439395:1-phenylpropanol; D013395:Sucrose; C072239:methyl jasmonate; C004931:1,1-diphenyl-2-picrylhydrazyl