title: Inhibitory effect of rape pollen supercritical CO2fluid extract against testosterone-induced benign prostatic hyperplasia in rats.
abstract: Benign prostatic hyperplasia (BPH) can lead to lower urinary tract symptoms. Rape pollen is an apicultural product that is composed of nutritionally valuable and biologically active substances. The aim of the present study was to investigate the protective effect of rape pollen supercritical CO2fluid extract (SFE-CO2) in BPH development using a testosterone-induced BPH rat model. BPH was induced in the experimental groups by daily subcutaneous injections of testosterone for a period of 30 days. Rape pollen SFE-CO2was administered daily by oral gavage concurrently with the testosterone injections. Animals were sacrificed at the scheduled termination and the prostates were weighed and subjected to histopathological examination. Testosterone, dihydrotestosterone (DHT), 5α-reductase and cyclooxygenase-2 (COX-2) levels were also measured. BPH-induced animals exhibited an increase in prostate weight with increased testosterone, DHT, 5α-reductase and COX-2 expression levels. However, rape pollen SFE-CO2treatment resulted in significant reductions in the prostate index and testosterone, DHT, 5α-reductase and COX-2 levels compared with those in BPH-induced animals. Histopathological examination also demonstrated that rape pollen SFE-CO2treatment suppressed testosterone-induced BPH. These observations indicate that rape pollen SFE-CO2inhibits the development of BPH in rats and these effects are closely associated with reductions in DHT, 5α-reductase and COX-2 levels. Therefore, the results of the present study clearly indicate that rape pollen SFE-CO2extract may be a useful agent in BPH treatment.
authors.affiliation: Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, P.R. China ; Key Laboratory of Women's Reproductive Health of Jiangxi Province, Jiangxi Maternal and Child Health Hospital, Nanchang, Jiangxi 330006, P.R. China.
authors.forename: Bi-Cheng
authors.initials: BC
authors.lastname: Yang
authors.affiliation: Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, P.R. China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Jin
authors.affiliation: Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, P.R. China.
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Yang
authors.affiliation: Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, P.R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.affiliation: Jiangxi Institute of Traditional Chinese Medicine, Nanchang, Jiangxi 330000, P.R. China.
authors.forename: Dan-Ming
authors.initials: DM
authors.lastname: Peng
keywords: 5α-reductase; benign prostatic hyperplasia; cyclooxygenase-2; rape pollen