title: Occurrence of Vibrio and Salmonella species in mussels (Mytilus galloprovincialis) collected along the Moroccan Atlantic coast.
abstract: This study reports the occurrence of different Vibrio and Salmonella species in 52 samples of Mytilus galloprovincialis collected from four sites along the Atlantic coast between Agadir and Essaouira (Anza, Cap Ghir, Imssouane and Essaouira). The level of Escherichia coli (E. coli) was also determined to evaluate the degree of microbial pollution in the investigated areas. In this study three methods were used : AFNOR NF EN ISO 6579 V08-013 for Salmonella spp., the provisional method routinely used by several laboratories (Institut Pasteur, Paris,…) for Vibrio cholerae and Vibrio parahaemolyticus in the seafood, and the most probable number method (MPN) using Norm ISO/TS 16649-3 (2005) for E. coli. The most frequently isolated Vibrios were Vibrio alginolyticus (90.4% of samples), followed by V. cholerae non O1 non O139 (15.4%) and V. parahaemolyticus (7.7%). Salmonella spp. was found in 15% of the samples. The number of E. coli ranged between 0.2/100 g and 1.8 10(3) /100 g of mussel soft tissues. This study indicates the potential sanitary risk associated with the presence of pathogenic bacteria in cultivated mussels in the two populous regions of southern Morocco, where shellfish production and maritime tourism are important to the local economy.
authors.affiliation: Faculty of Sciences, Laboratory of Biotechnology & Valorisation of Natural Resources, University Ibn Zohr, Agadir, Morocco.
authors.forename: Hasna
authors.initials: H
authors.lastname: Mannas
authors.affiliation: Faculty of Sciences, Laboratory of Biotechnology & Valorisation of Natural Resources, University Ibn Zohr, Agadir, Morocco.
authors.forename: Rachida
authors.initials: R
authors.lastname: Mimouni
authors.affiliation: Laboratory of Microbiology, National Health Security Food Office (ONSSA), Agadir, Morocco.
authors.forename: Noureddine
authors.initials: N
authors.lastname: Chaouqy
authors.affiliation: Faculty of Sciences, Laboratory of Biotechnology & Valorisation of Natural Resources, University Ibn Zohr, Agadir, Morocco.
authors.forename: Fatima
authors.initials: F
authors.lastname: Hamadi
authors.affiliation: Reader in Infection and Immunology Department of Biology and Biochemistry, University of Bath, BA2 7AY Bath, United Kingdom.
authors.forename: Jaime
authors.initials: J
authors.lastname: Martinez-Urtaza
keywords: Agadir; Escherichia coli; Essaouirra; Morocco; Mussels; Mytilus galloprovincialis; Salmonella; Vibrio