title: Characterization of Schizothorax prenanti cgnrhII gene: fasting affects cgnrhII expression.
abstract: In this study, the role of chicken gonadotropin-releasing hormone II (cgnrhII) in feeding regulation was investigated in Schizothorax prenanti. First, the full-length S. prenanti cgnrhII cDNA consisted of 693 bp with an open reading frame of 261 bp encoding a protein of 86 amino acids. Next, cgnrhII was widely expressed in the central and peripheral tissues. Last, there were significant changes in cgnrhII mRNA expression in the fasted group compared to the fed group in the S. prenanti hypothalamus during 24 h fasting (P < 0.05). Furthermore, the cgnrhII gene expression presented a significant decrease in the fasted group compared with the fed group (P < 0.05) on days 3, 5 and 7, after re-feeding, there was no significant changes in cgnrhII mRNA expression level between refed and fed group on day 9 (P > 0.05). Thus, the results suggest that cGnRH II expression is influenced by fasting and the gene may be involved in feeding regulation in S. prenanti.
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: D
authors.initials: D
authors.lastname: Yuan
authors.forename: C
authors.initials: C
authors.lastname: Zhou
authors.forename: F
authors.initials: F
authors.lastname: Lin
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: R
authors.initials: R
authors.lastname: Wei
authors.forename: Z
authors.initials: Z
authors.lastname: Xin
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: D
authors.initials: D
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Pu
authors.forename: Z
authors.initials: Z
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics; D004435:Eating / Q000235:genetics / Q000502:physiology*; D029941:Fish Proteins / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000235:genetics / Q000502:physiology; D007031:Hypothalamus / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone; C047717:LHRH, His(5)-Trp(7)-Tyr(8)-
keywords: distribution; feeding regulation; food deprivation