title: Biological characterization and pluripotent identification of sheep dermis-derived mesenchymal stem/progenitor cells.
abstract: Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were a multipotential stem cell population, which has potential applications in the tissue damage repair and skin transplant. Although a large number of studies deal with the human DMS/PCs self-renewal and regulation, however, the study of livestock-derived DMS/PCs has rarely been reported. Here, sheep DMS/PCs were isolated from one-month-old sheep embryos and studied at the cellular and molecular level. And then the DMS/PCs biological characteristics were analysed by RT-PCR and immunofluorescence. Experimental results showed that DMS/PCs could be expanded for 48 passages and the cells viability and hereditary character were steady. In addition, the DMS/PCs maker β -integrin, CD71, CD44, and CD73 were expressed positively through RT-PCR and immunofluorescence. Passage 3 DMS/PCs were successfully induced to differentiate into adipocytes, osteoblasts, chondrocytes, and neurocytes, respectively. The above results suggest that DMS/PCs not only have strong self-renewal capacity but also have the potential to differentiate into adipocytes, osteoblasts, chondrocytes, and neurocytes. The study provides theoretical basis and experimental evidence for potential clinical application.
authors.affiliation: Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Science, Beijing 100194, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cui
authors.affiliation: Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Science, Beijing 100194, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: He
authors.affiliation: Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Science, Beijing 100194, China.
authors.forename: Yabin
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Science, Beijing 100194, China.
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Science, Beijing 100194, China ; College of Pharmacy, Jiamusi University, Heilongjiang Province Key Laboratory of Biological Medicine Formulation, Jiamusi, Heilongjiang 154007, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Science, Beijing 100194, China.
authors.forename: Changli
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Science, Beijing 100194, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Guan
authors.affiliation: Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Science, Beijing 100194, China.
authors.forename: Xiangchen
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Science, Beijing 100194, China.
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Ma
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival; D019902:Chondrocytes / Q000166:cytology; D020405:Dermis / Q000166:cytology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology; D012756:Sheep