title: Shell extracts from the marine bivalve Pecten maximus regulate the synthesis of extracellular matrix in primary cultured human skin fibroblasts.
abstract: Mollusc shells are composed of more than 95% calcium carbonate and less than 5% of an organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. Previous studies have elucidated the biological activities of the shell matrices from bivalve molluscs on skin, especially on the expression of the extracellular matrix components of fibroblasts. In this work, we have investigated the potential biological activities of shell matrix components extracted from the shell of the scallop Pecten maximus on human fibroblasts in primary culture. Firstly, we demonstrated that shell matrix components had different effects on general cellular activities. Secondly, we have shown that the shell matrix components stimulate the synthesis of type I and III collagens, as well as that of sulphated GAGs. The increased expression of type I collagen is likely mediated by the recruitment of transactivating factors (Sp1, Sp3 and human c-Krox) in the -112/-61 bp COL1A1 promoter region. Finally, contrarily to what was obtained in previous works, we demonstrated that the scallop shell extracts have only a small effect on cell migration during in vitro wound tests and have no effect on cell proliferation. Thus, our research emphasizes the potential use of shell matrix of Pecten maximus for dermo-cosmetic applications.
authors.affiliation: UMR BOREA « Biologie des ORganismes et Ecosystèmes Aquatiques », MNHN, UPMC, UCBN, CNRS-7208, IRD-207, Université de Caen Basse-Normandie, IBFA, Campus 1, Science C, Caen cedex 5, France; Laboratoire Microenvironnement cellulaire et pathologies (MILPAT), EA 4652, SFR 146 ICORE, Université de Caen Basse-Normandie, Faculté de Médecine, CHU niveau 3, Caen cedex 5, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Latire
authors.affiliation: Laboratoire Microenvironnement cellulaire et pathologies (MILPAT), EA 4652, SFR 146 ICORE, Université de Caen Basse-Normandie, Faculté de Médecine, CHU niveau 3, Caen cedex 5, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Legendre
authors.affiliation: Laboratoire Microenvironnement cellulaire et pathologies (MILPAT), EA 4652, SFR 146 ICORE, Université de Caen Basse-Normandie, Faculté de Médecine, CHU niveau 3, Caen cedex 5, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bigot
authors.affiliation: Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules (ERRMECe), EA 1391, Institut des Matériaux, Université de Cergy-Pontoise, Cergy-Pontoise cedex, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Carduner
authors.affiliation: Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules (ERRMECe), EA 1391, Institut des Matériaux, Université de Cergy-Pontoise, Cergy-Pontoise cedex, France.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Kellouche
authors.affiliation: Laboratoire Microenvironnement cellulaire et pathologies (MILPAT), EA 4652, SFR 146 ICORE, Université de Caen Basse-Normandie, Faculté de Médecine, CHU niveau 3, Caen cedex 5, France.
authors.forename: Mouloud
authors.initials: M
authors.lastname: Bouyoucef
authors.affiliation: Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules (ERRMECe), EA 1391, Institut des Matériaux, Université de Cergy-Pontoise, Cergy-Pontoise cedex, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Carreiras
authors.affiliation: UMR 6282 CNRS "Biogéosciences", Université de Bourgogne, Dijon, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Marin
authors.affiliation: UMR BOREA « Biologie des ORganismes et Ecosystèmes Aquatiques », MNHN, UPMC, UCBN, CNRS-7208, IRD-207, Université de Caen Basse-Normandie, IBFA, Campus 1, Science C, Caen cedex 5, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Lebel
authors.affiliation: Laboratoire Microenvironnement cellulaire et pathologies (MILPAT), EA 4652, SFR 146 ICORE, Université de Caen Basse-Normandie, Faculté de Médecine, CHU niveau 3, Caen cedex 5, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Galéra
authors.affiliation: UMR BOREA « Biologie des ORganismes et Ecosystèmes Aquatiques », MNHN, UPMC, UCBN, CNRS-7208, IRD-207, Université de Caen Basse-Normandie, IBFA, Campus 1, Science C, Caen cedex 5, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Serpentini
mesh_terms: D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D005109:Extracellular Matrix / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D049910:Pecten / Q000737:chemistry*; D061251:Primary Cell Culture; D012867:Skin / Q000187:drug effects; D014020:Tissue Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D014020:Tissue Extracts