title: Tranilast, an orally active antiallergic compound, inhibits extracellular matrix production in human uterine leiomyoma and myometrial cells.
abstract: OBJECTIVE
  To determine the effect of tranilast (an antiallergic drug known to suppress fibrosis or to stabilize mast cells) on extracellular matrix production in human leiomyoma and myometrial cells.
  
  
  METHODS
  Laboratory study.
  
  
  METHODS
  University-affiliated laboratory.
  
  
  METHODS
  Seven premenopausal women who were admitted to the hospital for myomectomy or hysterectomy.
  
  
  METHODS
  Cells were treated with tranilast (300 μM) for 48 hours to measure extracellular matrix and activin-A expression by real-time reverse-transcription polymerase chain reaction and/or immunocytochemistry.
  
  
  METHODS
  The expression of fibronectin, collagen1A1, versican, and activin-A in myometrial and leiomyoma cells.
  
  
  RESULTS
  Tranilast decreased fibronectin, collagen 1A1, and versican messenger RNA (mRNA) expression in human primary leiomyoma cell culture. Similar results were found in an immortalized human leiomyoma cell line. Tranilast also decreased the mRNA expression of fibronectin, collagen 1A1, and versican in human primary myometrial cells. The reduced expression of fibronectin and collagen 1 were observed by immunocytochemistry as well. Tranilast also reduced profibrotic growth factor, activin-A mRNA expression in primary myometrial and leiomyoma cells.
  
  
  CONCLUSIONS
  Our results indicate that tranilast reduced fibronectin, collagen 1A1, versican, and activin-A expression in leiomyoma and myometrial cells, demonstrating its potential as an antifibrotic therapy for human leiomyomas.
authors.affiliation: Department of Experimental and Clinical Medicine, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy; Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi, Bangladesh.
authors.forename: Md Soriful
authors.initials: MS
authors.lastname: Islam
authors.affiliation: Department of Experimental and Clinical Medicine, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy.
authors.forename: Olga
authors.initials: O
authors.lastname: Protic
authors.affiliation: Department of Clinical Science, Polytechnic University of Marche, Ancona, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ciavattini
authors.affiliation: Department of Clinical Science, Polytechnic University of Marche, Ancona, Italy.
authors.forename: Stefano Raffaele
authors.initials: SR
authors.lastname: Giannubilo
authors.affiliation: Department of Clinical Science, Polytechnic University of Marche, Ancona, Italy.
authors.forename: Andrea Luigi
authors.initials: AL
authors.lastname: Tranquilli
authors.affiliation: Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
authors.forename: William H
authors.initials: WH
authors.lastname: Catherino
authors.affiliation: Department of Experimental and Clinical Medicine, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Castellucci
authors.affiliation: Department of Experimental and Clinical Medicine, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy; Department of Information Engineering, Polytechnic University of Marche, Ancona, Italy. Electronic address: p.ciarmela@univpm.it.
authors.forename: Pasquapina
authors.initials: P
authors.lastname: Ciarmela
mesh_terms: D028341:Activins / Q000378:metabolism; D000284:Administration, Oral; D000328:Adult; D018926:Anti-Allergic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D005355:Fibrosis; D006801:Humans; D007889:Leiomyoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D009215:Myometrium / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D053675:Versicans / Q000235:genetics / Q000378:metabolism; D062367:ortho-Aminobenzoates / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D018926:Anti-Allergic Agents; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D005353:Fibronectins; D012333:RNA, Messenger; C508322:VCAN protein, human; C422405:activin A; D062367:ortho-Aminobenzoates; D028341:Activins; D053675:Versicans; C012293:tranilast
keywords: Fibroid; activin-A; collagen 1A1; fibronectin; tranilast