title: Effect of BRCA1 immunohistochemical localizations on prognosis of patients with sporadic breast carcinomas.
abstract: Our purpose was to investigate the expression pattern of BRCA1 protein in sporadic breast carcinomas, as well as the clinicopathological and prognostic value of its subcellular localizations. Immunohistochemistry was performed on paraffin embedded tissue specimens from 111 sporadic, invasive breast carcinomas to detect the expression of the proteins BRCA1, ER, PR, erbB2, p53 and Ki67. BRCA1 protein was detected in the nuclei and the cytoplasm of the tumor cells. Nuclear BRCA1 immunoreactivity showed no relation with the classic clinicopathological markers and the expression of cerbB2, p53 and Ki67. Reduced expression of nuclear BRCA1 protein was found to exert an independent favorable impact on both the overall and relapse-free (RF) survival of the patients (p=0.019 and p=0.043, respectively). Cytoplasmic BRCA1 was associated with none of the classic histomorphological indices, except from the lymph node metastasis, with which its relation was found to be inverse (p=0.05), prolonging the RF survival of the patients (p=0.05). Our findings suggest that BRCA1 protein depicts different prognostic significance, according to its subcellular distribution. Nuclear detection of the protein was associated with a worse prognosis, while the cytoplasmic one was related to fewer recurrences as a result of fewer lymph node metastases.
authors.affiliation: 5th Department of Internal Medicine, Evagelismos Hospital, Athens, Greece.
authors.forename: Eleni
authors.initials: E
authors.lastname: Mylona
authors.affiliation: 1st Department of Pathology, Medical School, University of Athens, Athens, Greece.
authors.forename: Savvas
authors.initials: S
authors.lastname: Melissaris
authors.affiliation: 1st Department of Pathology, Medical School, University of Athens, Athens, Greece.
authors.forename: Alexandros
authors.initials: A
authors.lastname: Nomikos
authors.affiliation: 1st Department of Pathology, Medical School, University of Athens, Athens, Greece.
authors.forename: Irene
authors.initials: I
authors.lastname: Theohari
authors.affiliation: 1st Department of Pathology, Medical School, University of Athens, Athens, Greece.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Giannopoulou
authors.affiliation: 1st Department of Pathology, Medical School, University of Athens, Athens, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Tzelepis
authors.affiliation: 1st Department of Pathology, Medical School, University of Athens, Athens, Greece. Electronic address: lnako@med.uoa.gr.
authors.forename: Lydia
authors.initials: L
authors.lastname: Nakopoulou
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019313:BRCA1 Protein / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism
chemical_list: D019313:BRCA1 Protein; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone
keywords: BRCA1; Breast cancer; Prognosis; Sporadic; Survival