title: Suspected inflammatory cardiomyopathy. Prevalence of Borrelia burgdorferi in endomyocardial biopsies with positive serological evidence.
abstract: BACKGROUND
  Cardiac involvement in Lyme disease, caused by the tick-borne spirochete Borrelia burgdorferi, has been reported to occur in 0.3-4 % of infected patients in Europe. Cardiac manifestations may include conduction disturbances, and also myocarditis, pericarditis, and left ventricular dysfunction. We investigated the prevalence of B. burgdorferi DNA in endomyocardial biopsies from patients with suspected inflammatory heart disease and positive serology for B. burgdorferi.
  
  
  RESULTS
  In 64 patients, endomyocardial biopsies were taken after exclusion of coronary heart disease by coronary angiography, and investigated with polymerase chain reaction (PCR) for the presence of B. burgdorferi and cardiotropic viruses. B. burgdorferi DNA was not detected in any of the endomyocardial biopsies. Viruses, particularly parvovirus B19, were detected as infectious agents in 19 (30 %) patients.
  
  
  CONCLUSIONS
  The results of our study demonstrate that PCR analysis of endomyocardial biopsies from patients with suspected inflammatory heart disease, including individuals with dilated cardiomyopathy (DCM) and positive serology for B. burgdorferi, did not reveal the B. burgdorferi genome in any biopsy sample.
authors.affiliation: Department of Internal Medicine-Cardiology, Philipps-University Marburg, Baldingerstr., 35033, Marburg, Deutschland, karatoli@med.uni-marburg.de.
authors.forename: K
authors.initials: K
authors.lastname: Karatolios
authors.forename: B
authors.initials: B
authors.lastname: Maisch
authors.forename: S
authors.initials: S
authors.lastname: Pankuweit
mesh_terms: D025065:Borrelia burgdorferi / Q000302:isolation & purification; D009202:Cardiomyopathies / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D004716:Endometritis / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D005260:Female; D005858:Germany; D006801:Humans; D008193:Lyme Disease / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012307:Risk Factors; D012698:Serologic Tests