title: Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association.
abstract: Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1/POT1. We show that the fission yeast TPP1 ortholog, Tpz1, is SUMOylated. Tpz1 SUMOylation restricts telomere elongation and promotes Stn1/Ten1 telomere association, and a SUMO-Tpz1 fusion protein has increased affinity for Stn1. Our data suggest that SUMO inhibits telomerase through stimulation of Stn1/Ten1 action by Tpz1, highlighting the evolutionary conservation of the regulation of CST function by SUMOylation.
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK.
authors.forename: Mansi
authors.initials: M
authors.lastname: Garg
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK.
authors.forename: Resham L
authors.initials: RL
authors.lastname: Gurung
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK.
authors.forename: Sahar
authors.initials: S
authors.lastname: Mansoubi
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK.
authors.forename: Jubed O
authors.initials: JO
authors.lastname: Ahmed
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK.
authors.forename: Anoushka
authors.initials: A
authors.lastname: Davé
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK.
authors.forename: Felicity Z
authors.initials: FZ
authors.lastname: Watts
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK A.Bianchi@sussex.ac.uk.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bianchi
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D025842:SUMO-1 Protein / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism*; D000089804:Shelterin Complex; D058207:Sumoylation; D019098:Telomerase / Q000378:metabolism; D016615:Telomere / Q000378:metabolism*; D059505:Telomere Homeostasis; D034501:Telomere-Binding Proteins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D025842:SUMO-1 Protein; D029702:Schizosaccharomyces pombe Proteins; D000089804:Shelterin Complex; C523517:Stn1 protein, S pombe; D034501:Telomere-Binding Proteins; C529646:Tpz1 protein, S pombe; D019098:Telomerase
keywords: CST; SUMO; Stn1; Tpz1; telomeres