title: In vivo spectral and fluorescence microscopy comparison of microvascular function after treatment with OXi4503, Sunitinib and their combination in Caki-2 tumors.
abstract: Vascular targeting agents on their own have been shown to be insufficient for complete treatment of solid tumors, emphasizing the importance of studying the vascular effects of these drugs for their use with conventional therapies in the clinic. First-pass fluorescence imaging combined with hyperspectral imaging of hemoglobin saturation of microvessels in the murine dorsal window chamber model provides an easily implementable, low cost method to analyze tumor vascular response to these agents in real-time. In this study, the authors utilized these methods to spectroscopically demonstrate distinct vessel structure, blood flow and oxygenation changes in human Caki-2 renal cell carcinoma following treatment with OXi4503 alone, Sunitinib alone and both drugs together. We showed that treatment with OXi4503 plus Sunitinib destroyed existing tumor microvessels, inhibited blood vessel recovery and impaired Caki-2 tumor growth significantly more than either treatment alone.
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Biomedical Sciences Building, Gainesville, FL 32610, USA.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Lee
authors.affiliation: Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Cancer and Genetics Research Complex, Gainesville, FL 32610, USA.
authors.forename: Nikolett M
authors.initials: NM
authors.lastname: Biel
authors.affiliation: Ventana Medical Systems, Inc., Tucson, AZ 85755, USA.
authors.forename: Raymond T
authors.initials: RT
authors.lastname: Kozikowski
authors.affiliation: Department of Radiation Oncology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
authors.forename: Dietmar W
authors.initials: DW
authors.lastname: Siemann
authors.affiliation: Cancer Diagnosis Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, MD 20852, USA.
authors.forename: Brian S
authors.initials: BS
authors.lastname: Sorg
keywords: (100.2960) Image analysis; (170.0180) Microscopy; (170.2520) Fluorescence microscopy; (170.6280) Spectroscopy, fluorescence and luminescence