title: Brain proteomics of Anopheles gambiae.
abstract: Anopheles gambiae has a well-adapted system for host localization, feeding, and mating behavior, which are all governed by neuronal processes in the brain. However, there are no published reports characterizing the brain proteome to elucidate neuronal signaling mechanisms in the vector. To this end, a large-scale mapping of the brain proteome of An. gambiae was carried out using high resolution tandem mass spectrometry, revealing a repertoire of >1800 proteins, of which 15% could not be assigned any function. A large proportion of the identified proteins were predicted to be involved in diverse biological processes including metabolism, transport, protein synthesis, and olfaction. This study also led to the identification of 10 GPCR classes of proteins, which could govern sensory pathways in mosquitoes. Proteins involved in metabolic and neural processes, chromatin modeling, and synaptic vesicle transport associated with neuronal transmission were predominantly expressed in the brain. Proteogenomic analysis expanded our findings with the identification of 15 novel genes and 71 cases of gene refinements, a subset of which were validated by RT-PCR and sequencing. Overall, our study offers valuable insights into the brain physiology of the vector that could possibly open avenues for intervention strategies for malaria in the future.
authors.affiliation: 1 Institute of Bioinformatics , International Technology Park, Bangalore, Karnataka, India .
authors.forename: Sutopa B
authors.initials: SB
authors.lastname: Dwivedi
authors.forename: Babylakshmi
authors.initials: B
authors.lastname: Muthusamy
authors.forename: Praveen
authors.initials: P
authors.lastname: Kumar
authors.forename: Min-Sik
authors.initials: MS
authors.lastname: Kim
authors.forename: Raja Sekhar
authors.initials: RS
authors.lastname: Nirujogi
authors.forename: Derese
authors.initials: D
authors.lastname: Getnet
authors.forename: Priscilla
authors.initials: P
authors.lastname: Ahiakonu
authors.forename: Gourav
authors.initials: G
authors.lastname: De
authors.forename: Bipin
authors.initials: B
authors.lastname: Nair
authors.forename: Harsha
authors.initials: H
authors.lastname: Gowda
authors.forename: T S Keshava
authors.initials: TS
authors.lastname: Prasad
authors.forename: Nirbhay
authors.initials: N
authors.lastname: Kumar
authors.forename: Akhilesh
authors.initials: A
authors.lastname: Pandey
authors.forename: Mobolaji
authors.initials: M
authors.lastname: Okulate
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D019295:Computational Biology; D005260:Female; D023281:Genomics; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D016366:Open Reading Frames; D010455:Peptides; D014176:Protein Biosynthesis; D020543:Proteome; D040901:Proteomics / Q000379:methods; D016364:Reading Frames; D015203:Reproducibility of Results; D020506:Untranslated Regions
chemical_list: D019476:Insect Proteins; D010455:Peptides; D020543:Proteome; D020506:Untranslated Regions