title: [Quantitative mass spectrometry and its utilization in oncology].
abstract: Cancers are genetically and clinically very heterogeneous diseases; therefore, various proteomic studies have been trying to find bio-markers which can facilitate prognosis, diagnosis or treatment of these oncological diseases. The mass spectrometry is an effective tool for identification, quantitation, and characterization of biomolecules in the complex bio-logical samples. The first step suitable for selection of bio-markers called discovery proteomics provides a detailed analysis of the samples contributing to the identification of proteins, comparison of their presence in the samples, and selection of the convenient candidates for the prospective bio-markers. The next step of proteomics analysis is directed towards verification of chosen bio-markers with the approach called targeted proteomics. This technique evaluates presence and quantity of the proteins (biomarkers) in clinically precisely defined samples. This article focuses on the description of various approaches suitable for the quantitative analysis of the proteins connected with mass spectrometry.
authors.forename: L
authors.initials: L
authors.lastname: Hernychova
authors.forename: P
authors.initials: P
authors.lastname: Dvořáková
authors.forename: E
authors.initials: E
authors.lastname: Michalova
authors.forename: B
authors.initials: B
authors.lastname: Vojtěšek
mesh_terms: D015415:Biomarkers / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009369:Neoplasms / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics
chemical_list: D015415:Biomarkers; D011506:Proteins