title: Higher order structure characterization of protein therapeutics by hydrogen/deuterium exchange mass spectrometry.
abstract: Characterization of therapeutic drugs is a crucial step in drug development in the biopharmaceutical industry. Analysis of protein therapeutics is a challenging task because of the complexities associated with large molecular size and 3D structures. Recent advances in hydrogen/deuterium-exchange mass spectrometry (HDX-MS) have provided a means to assess higher-order structure of protein therapeutics in solution. In this review, the principles and procedures of HDX-MS for protein therapeutics characterization are presented, focusing on specific applications of epitope mapping for protein-protein interactions and higher-order structure comparison studies for conformational dynamics of protein therapeutics.
authors.affiliation: Bioanalytical and Discovery Analytical Sciences, Research and Development, Bristol-Myers Squibb, Route 206 and Province Line Road, Princeton, NJ, 08543, USA.
authors.forename: Richard Y-C
authors.initials: RY
authors.lastname: Huang
authors.forename: Guodong
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D041961:Deuterium Exchange Measurement / Q000379:methods*; D018604:Epitope Mapping / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins