title: Transplantation of embryonic and induced pluripotent stem cell-derived 3D retinal sheets into retinal degenerative mice.
abstract: In this article, we show that mouse embryonic stem cell- or induced pluripotent stem cell-derived 3D retinal tissue developed a structured outer nuclear layer (ONL) with complete inner and outer segments even in an advanced retinal degeneration model (rd1) that lacked ONL. We also observed host-graft synaptic connections by immunohistochemistry. This study provides a "proof of concept" for retinal sheet transplantation therapy for advanced retinal degenerative diseases.
authors.affiliation: Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan ; Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Juthaporn
authors.initials: J
authors.lastname: Assawachananont
authors.affiliation: Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Mandai
authors.affiliation: Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan ; Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Okamoto
authors.affiliation: Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Yamada
authors.affiliation: Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Mototsugu
authors.initials: M
authors.lastname: Eiraku
authors.affiliation: Electron Microscope Laboratory, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Yonemura
authors.affiliation: Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sasai
authors.affiliation: Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Masayo
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D053595:Embryonic Stem Cells / Q000166:cytology / Q000637:transplantation*; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000637:transplantation*; D007797:Laminin / Q000737:chemistry; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology; D011509:Proteoglycans / Q000737:chemistry; D018168:Receptors, Retinoic Acid / Q000037:antagonists & inhibitors; D012160:Retina / Q000378:metabolism / Q000473:pathology*; D012162:Retinal Degeneration / Q000628:therapy* / Q000662:veterinary; D014184:Transplantation, Homologous
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D018168:Receptors, Retinoic Acid; C059485:matrigel; D003094:Collagen