title: Differential expression of alpha-adrenoceptor subtypes in rat dorsal root ganglion after chronic constriction injury.
abstract: mRNAs of alpha-adrenoceptor (α-AR) subtypes are found in neurons in dorsal root ganglion (DRG) and change after peripheral nerve injury. In this study, the distribution of α-AR subtype proteins was studied in L5 DRG of normal rats and rats with chronic constriction injury of sciatic nerve (CCI). Using immunofluorescence technique, it was found that α1A-, α1B-, and α2A-AR proteins were expressed in large, medium, and small size neurons in normal DRG, and significantly increased in all size neurons 14 days after CCI. α1D- and α2C-AR was also expressed in all size neurons in normal DRG. However, α1D-AR was significantly increased and α2C-AR was decreased in small size neurons 14 days post CCI. α2B-AR neurons were not detectable in normal and CCI DRG. Co-expression of α1A- and α2A-AR in the same neuron was observed in normal DRG and increased post CCI. Collectively, these results indicated that there is distinct distribution of α-AR subtypes in DRG neurons, and the distribution and levels of expression of α-AR subtypes change differently after CCI. The up-regulation of α-AR subtypes in DRG neurons may play an important role in the process of generating and transmitting neuropathic pain.
authors.affiliation: Department of Physiology, Basic Medical School of Wuhan University, Wuhan, 430071, China.
authors.forename: Hong-Ju
authors.initials: HJ
authors.lastname: Cheng
authors.affiliation: Electrophysiological Laboratory, Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi, 832002, China.
authors.forename: Ke-Tao
authors.initials: KT
authors.lastname: Ma
authors.affiliation: Electrophysiological Laboratory, Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi, 832002, China. lili@shzu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Electrophysiological Laboratory, Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi, 832002, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Physiology, Basic Medical School of Wuhan University, Wuhan, 430071, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Physiology, Basic Medical School of Wuhan University, Wuhan, 430071, China.|Electrophysiological Laboratory, Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi, 832002, China.|Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Jun-Qiang
authors.initials: JQ
authors.lastname: Si
mesh_terms: D000818:Animals; D048429:Cell Size; D002908:Chronic Disease; D003251:Constriction, Pathologic; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000473:pathology; D008297:Male; D018613:Microscopy, Confocal; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010147:Pain Measurement / Q000379:methods; D017288:Pain Threshold; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D018341:Receptors, Adrenergic, alpha-2 / Q000378:metabolism*; D012584:Sciatic Nerve / Q000293:injuries* / Q000601:surgery
chemical_list: D020033:Protein Isoforms; D018340:Receptors, Adrenergic, alpha-1; D018341:Receptors, Adrenergic, alpha-2