title: Determination of 15 sedative residues in mutton by rapid resolution liquid chromatography-tandem mass spectrometry.
abstract: BACKGROUND
  The use of xenobiotic compounds in animal husbandry has given rise to consumer anxieties regarding residual risk and food safety. Thus, animal tissues have become main samples for residue analysis and food safety for sedatives. In this study, a rapid resolution liquid chromatography-tandem mass spectrometry (RRLC-MS/MS) method was established for the determination of 15 sedatives residues in mutton.
  
  
  RESULTS
  After enzymolysis, sedatives residues in mutton were extracted by ammonium hydroxide-acetonitrile (10:90, v/v) and determined by RRLC-MS/MS with quantification by standard curve method. The calibration curves showed good linearity within the concentrations of 0.5-50 µg kg(-1) with the correlation coefficients (r(2)) ranged from 0.9639 to 0.9984. The limits of detection (LODs) and quantification (LOQs) were 0.25-2.5 and 0.5-5 µg kg(-1), respectively. The average recoveries of spikes samples were in the ranges of 74.1-116.8% with relative standard deviations of intra- and inter-day ranged from 2.6% to 11.2% and from 2.1% to 11.4%, respectively.
  
  
  CONCLUSIONS
  This method is simple, sensitive and accurate in the determination of sedative residues.
authors.affiliation: Gansu Agricultural University/Gansu Key Laboratory of Herbivorous Animal Biotechnology, 730070, Lanzhou, China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Wei
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Luo
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Suli
authors.initials: S
authors.lastname: Fang
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D059022:Food Safety / Q000379:methods*; D006801:Humans; D006993:Hypnotics and Sedatives / Q000032:analysis*; D057230:Limit of Detection; D012756:Sheep; D053719:Tandem Mass Spectrometry / Q000379:methods*; D015262:Xenobiotics / Q000032:analysis*
chemical_list: D006993:Hypnotics and Sedatives; D015262:Xenobiotics
keywords: mutton; rapid resolution liquid chromatography-tandem mass spectrometry (RRLC-MS/MS); residues; sedatives