title: ins-7 Gene expression is partially regulated by the DAF-16/IIS signaling pathway in Caenorhabditis elegans under celecoxib intervention.
abstract: DAF-16 target genes are employed as reporters of the insulin/IGF-1 like signal pathway (IIS), and this is notably true when Caenorhabditis elegans (C. elegans) is used to study the action of anti-aging compounds on IIS activity. However, some of these genes may not be specific to DAF-16, even if their expression levels are altered when DAF-16 is activated. Celecoxib was reported to extend the lifespan of C. elegans through activation of DAF-16. Our results confirmed the function of celecoxib on aging; however, we found that the expression of ins-7, a DAF-16 target gene, was abnormally regulated by celecoxib. ins-7 plays an important role in regulating aging, and its expression is suppressed in C. elegans when DAF-16 is activated. However, we found that celecoxib upregulated the expression of ins-7 in contrast to its role in DAF-16 activation. Our subsequent analysis indicated that the expression level of ins-7 in C. elegans was negatively regulated by DAF-16 activity. Additionally, its expression was also positively regulated by DAF-16-independent mechanisms, at least following external pharmacological intervention. Our study suggests that ins-7 is not a specific target gene of DAF-16, and should not be chosen as a reporter for IIS activity. This conclusion is important in the study of INSs on aging in C. elegans, especially under the circumstance of drug intervention.
authors.affiliation: Sericulture & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, China.
authors.forename: Shanqing
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Sericulture & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, China.
authors.forename: Sentai
authors.initials: S
authors.lastname: Liao
authors.affiliation: Sericulture & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, China.
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Sericulture & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Qu
authors.affiliation: Sericulture & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D000068579:Celecoxib; D051858:Forkhead Transcription Factors; D005786:Gene Expression Regulation / Q000187:drug effects*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D008136:Longevity / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D036361:Peptide Hormones / Q000235:genetics* / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology*; D034622:RNA Interference / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013449:Sulfonamides / Q000494:pharmacology*; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D029742:Caenorhabditis elegans Proteins; D051858:Forkhead Transcription Factors; C525653:Ins-7 protein, C elegans; D007328:Insulin; C583955:PQM-1 protein, C elegans; D036361:Peptide Hormones; D011720:Pyrazoles; D013449:Sulfonamides; D015534:Trans-Activators; D014157:Transcription Factors; C108831:daf-16 protein, C elegans; D049452:Green Fluorescent Proteins; D007334:Insulin-Like Growth Factor I; D000068579:Celecoxib