title: Evaluation of the humoral immune response to human leukocyte antigens in Brazilian renal transplant candidates.
abstract: Pre-transplant sensitization to human leukocyte antigens (HLA) is a risk factor for graft failure. Studies of the immunological profile related to anti-HLA antibodies in Brazilian renal transplant candidates are few. In this study, we evaluated the humoral immune response to HLA antigens in 269 renal transplant candidates, in Paraná State, Brazil. The HLA typing was performed by the polymerase chain reaction sequence-specific oligonucleotide method (PCR-SSO) combined with Luminex technology, using an SSO-LABType commercial kit (One Lambda, Inc., Canoga Park, CA, USA). The percentages of panel-reactive antibodies (PRA) and the specificity of anti-HLA antibodies were determined using the LS1PRA and LS2PRA commercial kits (One Lambda, Inc.). The PRA-positive group consisted of 182 (67.7%) patients, and the PRA-negative group of 87 (32.3%) patients. The two groups differed significantly only with respect to gender. Females were the most sensitized. Among the 182 patients with PRA- positive, 62 (34.1%) were positive for class I and negative for class II, 39 (21.4%) were negative for class I and positive for class II, and 81 (44.5%) were positive for both classes I and II. The HLA-A*02, A*24, A*01, B*44, B*35, B*15, DRB1*11, DRB1*04 and DRB1*03 allele groups were the most frequent. The specificities of anti-HLA antibodies were more frequent: A34, B57, Cw15, Cw16, DR51, DQ8 and DP14. This study documented the profile of anti-HLA antibodies in patients with chronic renal failure who were on waiting lists for an organ in Paraná, and found high sensitization to HLA antigens in the samples.
authors.affiliation: Department of Basic Health Sciences, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Patricia Keiko
authors.initials: PK
authors.lastname: Saito
authors.affiliation: Department of Basic Health Sciences, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Roger Haruki
authors.initials: RH
authors.lastname: Yamakawa
authors.affiliation: Histogene Laboratory of Histocompatibility and Genetics, Maringá, Paraná, Brazil.
authors.forename: Erica Pereira
authors.initials: EP
authors.lastname: Aparecida
authors.affiliation: Department of Statistics, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Waldir Verissimo
authors.initials: WV
authors.lastname: da Silva Júnior
authors.affiliation: Department of Basic Health Sciences, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Sueli Donizete
authors.initials: SD
authors.lastname: Borelli
mesh_terms: D000328:Adult; D000368:Aged; D001938:Brazil / Q000453:epidemiology; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D056724:Immunity, Humoral; D007676:Kidney Failure, Chronic / Q000453:epidemiology / Q000276:immunology* / Q000628:therapy*; D016030:Kidney Transplantation / Q000706:statistics & numerical data; D008297:Male; D008875:Middle Aged; D014850:Waiting Lists
chemical_list: D006680:HLA Antigens