title: Variations in body weight, food intake and body composition after long-term high-fat diet feeding in C57BL/6J mice.
abstract: OBJECTIVE
  To investigate the variations in body weight, food intake, and body composition of both male and female C57BL/6J mice during a diet-induced obesity model with high-fat diet (HFD) feeding.
  
  
  METHODS
  Mice were individually housed and fed ad libitum either a low-fat diet (LFD, 10% calories from fat; n = 15 male, n = 15 female) or HFD (45% calories from fat; n = 277 male, n = 278 female) from 8 to 43 weeks of age. Body weight, food intake, and body composition were routinely measured.
  
  
  RESULTS
  Body weight was significantly increased with HFD (vs. LFD) in males from week 14 (P = 0.0221) and in females from week 27 (P = 0.0076). Fat mass and fat-free mass of all groups were significantly increased over time (all P < 0.0001), with a large variation observed in fat mass. Baseline fat mass, fat-free mass, and daily energy intake were significant predictors of future body weight for both sexes (P < 0.0001). Baseline fat mass was a significant predictor of future body fat (P < 0.0001).
  
  
  CONCLUSIONS
  Both males and females have large variations in fat mass, and this variability increases over time, while that of fat-free mass remains relatively stable. Sex differences exist in HFD responses and multivariate predicting models of body weight.
authors.affiliation: Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, Alabama, USA; Nutrition Obesity Research Center, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Yang
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Smith
authors.forename: Karen D
authors.initials: KD
authors.lastname: Keating
authors.forename: David B
authors.initials: DB
authors.lastname: Allison
authors.forename: Tim R
authors.initials: TR
authors.lastname: Nagy
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D001823:Body Composition; D001835:Body Weight / Q000502:physiology*; D059305:Diet, High-Fat; D004435:Eating; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015999:Multivariate Analysis; D013997:Time Factors