title: Campylobacter jejuni in commercial eggs.
abstract: This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health.
authors.affiliation: Laboratório de Biotecnologia Animal Aplicada Faculdade de Medicina Veterinária Universidade Federal de Uberlândia UberlândiaMG Brazil ; Instituto de Ciências Biomédicas Universidade Federal de Uberlândia UberlândiaMG Brazil.
authors.forename: Belchiolina Beatriz
authors.initials: BB
authors.lastname: Fonseca
authors.affiliation: Instituto de Ciências Biomédicas Universidade Federal de Uberlândia UberlândiaMG Brazil.
authors.forename: Marcelo Emílio
authors.initials: ME
authors.lastname: Beletti
authors.affiliation: Laboratório de Biotecnologia Animal Aplicada Faculdade de Medicina Veterinária Universidade Federal de Uberlândia UberlândiaMG Brazil.
authors.forename: Roberta Torres
authors.initials: RT
authors.lastname: de Melo
authors.affiliation: Laboratório de Biotecnologia Animal Aplicada Faculdade de Medicina Veterinária Universidade Federal de Uberlândia UberlândiaMG Brazil.
authors.forename: Eliane Pereira
authors.initials: EP
authors.lastname: Mendonça
authors.affiliation: Laboratório de Biotecnologia Animal Aplicada Faculdade de Medicina Veterinária Universidade Federal de Uberlândia UberlândiaMG Brazil.
authors.forename: Letícia Ríspoli
authors.initials: LR
authors.lastname: Coelho
authors.affiliation: Laboratório de Biotecnologia Animal Aplicada Faculdade de Medicina Veterinária Universidade Federal de Uberlândia UberlândiaMG Brazil.
authors.forename: Priscila Christen
authors.initials: PC
authors.lastname: Nalevaiko
authors.affiliation: Laboratório de Biotecnologia Animal Aplicada Faculdade de Medicina Veterinária Universidade Federal de Uberlândia UberlândiaMG Brazil.
authors.forename: Daise Aparecida
authors.initials: DA
authors.lastname: Rossi
mesh_terms: D000818:Animals; D016123:Campylobacter jejuni / Q000302:isolation & purification* / Q000502:physiology*; D004531:Eggs / Q000382:microbiology*; D006801:Humans; D050296:Microbial Viability
keywords: campylobacteriosis; commercial eggs; penetration