title: [Value of second-time invasive prenatal diagnosis in the current circumstances].
abstract: OBJECTIVE
  To assess the safety of repeated invasive prenatal diagnosis primarily due to failed culture of amniotic cells.
  
  
  METHODS
  Between January 2000 and October 2012, 167 cases required repeated invasive prenatal diagnosis among a total of 5304 amniocentesis cases. Clinical outcome and karyotypes were analyzed to calculate the rate of fetal loss.
  
  
  RESULTS
  For the 167 re-sampled cases, the indications have included failed amniocyte culture (121 cases), chromosome mosaicisms (23 cases), failed amniocentesis (21 cases), and request for confirmation (2 cases). No fetal loss has occurred. All samples were cultured successfully. Fourteen cases (8.38%) have been found with an abnormal karyotype. Four mosaic trisomic cases (2 mosaic trisomy 16, 1 mosaic trisomy 20, and 1 mosaic trisomy 8) were verified to be normal.
  
  
  CONCLUSIONS
  Repeated invasive prenatal diagnosis does not increase the rate of fetal loss. It can be recommended to cases with failed amniocyte culture. Caution should be undertaken when counseling prenatally detected mosaicism trisomies.
authors.affiliation: Prenatal Diagnosis Center, International Peace Maternal and Child Health Hospital, Shanghai Jiao Tong University, Shanghai 200030, P.R. China. Email: wyanlin@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Wang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Cheng
authors.forename: Jingbo
authors.initials: J
authors.lastname: Yu
authors.forename: Chunmin
authors.initials: C
authors.lastname: Liu
mesh_terms: D000328:Adult; D000649:Amniocentesis; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008875:Middle Aged; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D014314:Trisomy; D055815:Young Adult