title: Association of polymorphisms in the 5' untranslated region of RAD51 gene with risk of endometrial cancer in the Polish population.
abstract: OBJECTIVE
  Many of the studies have analyzed cell repair capabilities, following cancer development. The cellular reaction to DNA damaging agents can modulate the susceptibility to various tumors. This reaction is mainly determined by DNA repair efficacy which, in turn, may be influenced by the variability of DNA repair genes, expressed by their polymorphisms.
  
  
  METHODS
  This report describes studies of the distribution of genotypes and the frequency of alleles of the G135C (rs1801320) and G172T (rs1801321) RAD51 polymorphism in 630 paraffin-embedded samples of tumor tissue from patients with endometrial cancer. DNA from 630 normal endometrial tissues served as control. RAD51 polymorphisms were determined by PCR-RFLP.
  
  
  RESULTS
  In the present work, a relationship was identified between RAD51 G135C polymorphism and the incidence of endometrial cancer. Endometrial cancer patients had an overrepresentation of 135C allele. The 135C/C homozygous variant increased cancer risk. A tendency towards a decreased risk of endometrial cancer was observed with the occurrence of combined G135C-G172G genotype of RAD51 polymorphism. An association was confirmed between RAD51 G135C and G172T polymorphisms and endometrial cancer progression, assessed by the histological grades.
  
  
  CONCLUSIONS
  The results support the hypothesis that RAD51 G135C and G172T polymorphisms may be associated with endometrial cancer occurrence and/or progression.
authors.affiliation: Department of Obstetrics and Gynaecology, Regional Hospital in Kalisz, Kalisz, Poland.
authors.forename: Magdalena M
authors.initials: MM
authors.lastname: Michalska
authors.forename: Dariusz
authors.initials: D
authors.lastname: Samulak
authors.forename: Hanna
authors.initials: H
authors.lastname: Romanowicz
authors.forename: Beata
authors.initials: B
authors.lastname: Smolarz
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D016022:Case-Control Studies; D004260:DNA Repair / Q000235:genetics; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015994:Incidence; D008875:Middle Aged; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D051135:Rad51 Recombinase / Q000235:genetics* / Q000378:metabolism; D012307:Risk Factors; D044465:White People / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D004273:DNA, Neoplasm; C495153:RAD51 protein, human; D051135:Rad51 Recombinase