title: Biosurfactant produced by novel Pseudomonas sp. WJ6 with biodegradation of n-alkanes and polycyclic aromatic hydrocarbons.
abstract: Alkanes and polycyclic aromatic hydrocarbons (PAHs) have threatened the environment due to toxicity and poor bioavailability. Interest in degradation of these hazardous materials by biosurfactant-producing bacteria has been steadily increasing in recent years. In this work, a novel biosurfactant-producing Pseudomonas sp. WJ6 was isolated to degrade a wide range of n-alkanes and polycyclic aromatic hydrocarbons. Production of lipopeptide biosurfactant was observed in all biodegradable studies. These lipopeptides were purified and identified by C18 RP-HPLC system and electrospray ionization-mass spectrometry. Results of structural analysis showed that these lipopeptides generated from different hydrocarbons were classified to be surfactin, fengycin and lichenysin. Heavy-oil sludge washing experiments demonstrated that lipopeptides produced by Pseudomonas sp. WJ6 have 92.46% of heavy-oil washing efficiency. The obtained results indicate that this novel bacterial strain and its lipopeptides have great potentials in the environmental remediation and petroleum recovery.
authors.affiliation: Power Environmental Energy Research Institute, Covina, CA 91722, USA; Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Science, Beijing 100190, PR China; Institute of Porous Flow & Fluid Mechanics, Chinese Academy of Sciences, Langfang 065007, PR China. Electronic address: wenjie.hsia@gmail.com.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Xia
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Science, Beijing 100190, PR China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Du
authors.affiliation: Institute of Porous Flow & Fluid Mechanics, Chinese Academy of Sciences, Langfang 065007, PR China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Dong
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Science, Beijing 100190, PR China. Electronic address: fuyi.wang@iccas.ac.cn.
authors.forename: Fuyi
authors.initials: F
authors.lastname: Wang
authors.affiliation: Power Environmental Energy Research Institute, Covina, CA 91722, USA.
authors.forename: Panqing
authors.initials: P
authors.lastname: He
authors.affiliation: Power Environmental Energy Research Institute, Covina, CA 91722, USA. Electronic address: yongchun.tang@peeri.org.
authors.forename: YongChun
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000473:Alkanes / Q000378:metabolism*; D001673:Biodegradation, Environmental; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D000473:Alkanes; D011084:Polycyclic Aromatic Hydrocarbons; D013501:Surface-Active Agents
keywords: Aromatic hydrocarbons; Biodegradation; Lipopeptides; Pseudomonas; n-Alkane