title: Mesenchymal stem cells modulate release of matrix proteins from tendon surfaces in vitro: a potential beneficial therapeutic effect.
abstract: OBJECTIVE
  Injury of tendons contained within a synovial environment, such as joint, bursa or tendon sheath, frequently fails to heal and releases matrix proteins into the synovial fluid, driving inflammation. This study investigated the effectiveness of cells to seal tendon surfaces and provoke matrix synthesis as a possible effective injectable therapy.
  
  
  METHODS
  Equine flexor tendon explants were cultured overnight in suspensions of bone marrow and synovium-derived mesenchymal stems cells and, as controls, two sources of fibroblasts, derived from tendon and skin, which adhered to the explants. Release of the most abundant tendon extracellular matrix proteins into the media was assayed, along with specific matrix proteins synthesis by real-time PCR.
  
  
  RESULTS
  Release of extracellular matrix proteins was influenced by the coating cell type. Fibroblasts from skin and tendon appeared less capable of preventing the release of matrix proteins than mesenchymal stems cells.
  
  
  CONCLUSIONS
  The source of cell is an important consideration for cell therapy.
authors.affiliation: The Royal Veterinary College, Department of Clinical Sciences & Services, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire, AL9 7TA, UK.
authors.forename: Elaine R
authors.initials: ER
authors.lastname: Garvican
authors.forename: Jayesh
authors.initials: J
authors.lastname: Dudhia
authors.forename: Ana-Liz
authors.initials: AL
authors.lastname: Alves
authors.forename: Lucy E
authors.initials: LE
authors.lastname: Clements
authors.forename: Francois Du
authors.initials: FD
authors.lastname: Plessis
authors.forename: Roger K W
authors.initials: RK
authors.lastname: Smith
mesh_terms: D000818:Animals; D003470:Culture Media / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D006736:Horses; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D060888:Real-Time Polymerase Chain Reaction; D012044:Regression Analysis; D013582:Synovial Fluid / Q000378:metabolism*; D013708:Tendon Injuries / Q000378:metabolism* / Q000628:therapy; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media; D017931:DNA Primers; D016326:Extracellular Matrix Proteins
keywords: equine; mesenchymal stem cell; synovial fluid; tendon