title: Protein-poly(amino acid) complex precipitation for high-concentration protein formulation.
abstract: A method for concentration of protein solutions is required for high-dosage protein formulation. Here, we present a precipitation-redissolution method by poly(amino acid) for proteins, including therapeutic enzymes, antibodies, and hormones. The proteins were fully precipitated by the addition of poly-L-lysine or poly-L-glutamic acid at low ionic strength, after which precipitate was dissolved at physiological ionic strength. The activities and secondary structures of redissolved proteins, especially antibodies, were almost identical to the native state. The precipitation-redissolution method is a simple and rapid technique for concentration of protein formulations.
authors.affiliation: Faculty of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8573, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kurinomaru
authors.forename: Takuya
authors.initials: T
authors.lastname: Maruyama
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Izaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Handa
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Kimoto
authors.forename: Kentaro
authors.initials: K
authors.lastname: Shiraki
mesh_terms: D011232:Chemical Precipitation; D011099:Polyglutamic Acid / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012995:Solubility
chemical_list: D011506:Proteins; D011107:Polylysine; D011099:Polyglutamic Acid
keywords: complexation; dissolution; polyelectrolytes; precipitation; protein formulation; proteins