title: Protective effects of the combination of sodium ferulate and oxymatrine on cecal ligation and puncture-induced sepsis in mice.
abstract: The aim of this study was to investigate the effects of the combination of sodium ferulate (SF) and oxymatrine (OMT) on mice with cecal ligation and puncture (CLP)-induced sepsis. Swiss male mice were randomly divided into a control group, CLP group, three SF + OMT groups (3.1+6.9; 6.2+13.8 and 12.3+27.7 mg/kg), SF (6.2 mg/kg) group and OMT (13.8 mg/kg) group. Eight hours after the administration of the drugs, the survival rates and survival times of the animals were monitored. In addition, the lung wet/dry weight (W/D) ratio; alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) levels in the serum; the C-reactive protein (CRP), interleukin-6 (IL-6) and interferon-γ (IFN-γ) levels in the serum and lung and liver homogenates; and the malondialdehyde (MDA) and superoxidase dismutase (SOD) levels in the lung and liver homogenates were measured. The bacterial load in the serum was also studied. Following treatment with the combination of SF and OMT, the survival rate increased and the survival time was prolonged; CLP-induced increases in the lung W/D ratio and the levels of ALT, AST, LDH, CRP, IL-6, IFN-γ and MDA were significantly reduced; and the SOD activity levels were increased, compared with those of the untreated animals with CLP-induced sepsis. These results indicated that the combination of SF and OMT induced protective effects against CLP-induced lethal sepsis of mice. The possible mechanism of these effects may be associated with the alleviation of systemic inflammation and diminishment of oxidative injury.
authors.affiliation: School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China.
authors.forename: Mengxin
authors.initials: M
authors.lastname: Xu
authors.affiliation: School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Pei
authors.affiliation: School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China.
authors.forename: Songmei
authors.initials: S
authors.lastname: Sun
authors.affiliation: Beijing SL Pharmaceutical Co., Ltd., Beijing 100049, P.R. China.
authors.forename: Mingbo
authors.initials: M
authors.lastname: Xu
authors.affiliation: School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Liu
keywords: inflammation; oxymatrine sepsis; sodium ferulate