title: Analysis of circulating microRNAs that are specifically increased in hyperlipidemic and/or hyperglycemic sera.
abstract: MicroRNAs (miRNAs) are small non-coding RNA sequences that regulate gene expression post-transcriptionally by translation inhibition or mRNA degradation. The aim of the present study was to analyze serum miRNAs modulated by hyperlipidemia and/or hyperglycemia and to correlate them with biochemical parameters within lipid metabolism. Five selected circulating miRNAs (miR-125a-5p, miR-146a, miR-10a, miR-21 and miR-33a) were individually analyzed by TaqMan miRNA assays along with lipid and inflammation parameters in sera from 20 hyperlipidemic (HL) and/or hyperglycemic (HG) patients, and compared with data from five normolipidemic/normoglycemic subjects. Results showed: (1) the levels of all the analyzed circulating miRNA were increased in HL sera and correlated positively with sera's lipid and inflammatory parameters; (2) circulating miR-125a-5p and miR-146a levels were increased in HG and/or HL sera; (3) all selected miRNAs were detected in α-lipoprotein fraction from sera, and miR-33a was also present in β-lipoprotein fraction; (4) miRNA concentrations were increased in the α-lipoprotein fraction from HL sera. These data show a statistically significant correlation of the analyzed miRNA with increased lipids, specifically with α- and β-lipoproteins, and CRP and IL-1β levels in HL and/or HG sera, suggesting a contribution of these miRNAs to the atherosclerotic process.
authors.affiliation: Lipidomics Department, Institute of Cellular Biology and Pathology "Nicolae Simionescu", Romanian Academy, 8, B.P. Hasdeu Street, 050568, Bucharest, Romania.
authors.forename: Natalia
authors.initials: N
authors.lastname: Simionescu
authors.forename: Loredan S
authors.initials: LS
authors.lastname: Niculescu
authors.forename: Gabriela M
authors.initials: GM
authors.lastname: Sanda
authors.forename: Denisa
authors.initials: D
authors.lastname: Margina
authors.forename: Anca V
authors.initials: AV
authors.lastname: Sima
mesh_terms: D000328:Adult; D000368:Aged; D053299:Apolipoprotein B-100 / Q000097:blood; D001057:Apolipoproteins E / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D002097:C-Reactive Protein / Q000378:metabolism; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D005260:Female; D006801:Humans; D006943:Hyperglycemia / Q000097:blood*; D006949:Hyperlipidemias / Q000097:blood*; D053583:Interleukin-1beta / Q000097:blood; D016014:Linear Models; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood
chemical_list: D053299:Apolipoprotein B-100; D001057:Apolipoproteins E; D001786:Blood Glucose; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D053583:Interleukin-1beta; C547183:MIRN10 microRNA, human; C531888:MIRN125 microRNA, human; C542542:MIRN146 microRNA, human; C524940:MIRN21 microRNA, human; C550416:MIRN33a microRNA, human; D035683:MicroRNAs; D014280:Triglycerides; D002097:C-Reactive Protein