title: Chromatin immunoprecipitation protocol for mammalian cells.
abstract: The genomic approach (ChIP-seq) we introduce here is now a widely used powerful tool to explore protein-DNA interaction at genome-wide level in high resolution. This technology opens up the way to understand how local event mediated by protein-protein or protein-DNA interactions lead to the dynamic changes of overall chromosome structure and how variety of proteins make a regulatory network for the faithful execution of various chromosomal functions (i.e., transcription, replication, recombination, repair, and partition).
authors.affiliation: Laboratory of Genome Structure and Function, Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Komata
authors.forename: Yuki
authors.initials: Y
authors.lastname: Katou
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Nakato
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Shirahige
authors.forename: Masashige
authors.initials: M
authors.lastname: Bando
mesh_terms: D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans
chemical_list: D002843:Chromatin; D003432:Cross-Linking Reagents; D004247:DNA