title: Protein biomarkers for enhancement of radiation dose and injury assessment in nonhuman primate total-body irradiation model.
abstract: Development and validation of early-response radiation injury biomarkers are critical for effective triage and medical management of irradiated individuals. Plasma protein and haematological profiles were evaluated using multivariate linear-regression analysis to provide dose-response calibration curves for photon-radiation dose assessment in 30 rhesus macaques total-body-irradiated to 1-8.5 Gy with (60)Co gamma rays (0.55 Gy min(-1)). Equations for radiation dose received were established based on different combinations of protein biomarkers [i.e. C-reactive protein (CRP), serum amyloid A (SAA), interleukin 6 (IL-6) and Flt3 Ligand (Flt3L)] at samples collection time-points 6 h, 1, 2, 3, 4 and 7 d post-total-body irradiation. Dynamic changes in the levels of CRP, SAA, IL-6 and Flt3L may function as prognostic indicators of the time course and severity of acute radiation sickness (ARS). The combination of protein biomarkers provides greater accuracy for early radiation assessment than any one biomarker alone.
authors.affiliation: Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5693, USA natalia.ossetrova@usuhs.edu.
authors.forename: Natalia I
authors.initials: NI
authors.lastname: Ossetrova
authors.affiliation: Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5693, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Sandgren
authors.affiliation: Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5693, USA.
authors.forename: William F
authors.initials: WF
authors.lastname: Blakely
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D004307:Dose-Response Relationship, Radiation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005720:Gamma Rays; D008253:Macaca mulatta; D008297:Male; D020543:Proteome / Q000032:analysis* / Q000528:radiation effects*; D040901:Proteomics / Q000379:methods; D011832:Radiation Injuries / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D011874:Radiometry; D014916:Whole-Body Irradiation / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome