title: Interaction of fengycin with stratum corneum mimicking model membranes: a calorimetry study.
abstract: Based on its outstanding antifungal properties, it is reasonable to believe that fengycin might be efficient to topically treat localized dermatomycoses. Since most of the fungi species involved in the formation of those mycotic skin diseases colonize primarily the stratum corneum (SC), studying the interaction between fengycin and SC-mimicking lipid membranes is a primary step to determine the potential of fengycin to overcome the physical barrier of the skin. In this respect, multilamellar lipid vesicles (MLVs), with a lipid composition mimicking that of the SC, were prepared and characterized by differential scanning calorimetry (DSC). The critical micelle concentration (CMC) of fengycin was also assessed under skin conditions and found to be 1.2±0.1μM. The molecular interactions of fengycin with SC-mimicking MLVs were investigated by both DSC and isothermal titration calorimetry (ITC). Results showed that the interactions were considerably affected by changes in lipid phase behaviour. At 40°C and below, fengycin induced exothermic changes in the lipid structures suggesting that less-ordered lipid domains became more-ordered in presence of fengycin. At 60°C, clearly endothermic interaction enthalpies were observed, which could arise from the "melting" of remaining solid domains enriched in high melting lipids that without fengycin melt at higher temperatures.
authors.affiliation: Université de Liège, Gembloux Agro-Bio-Tech, Unité de Chimie Biologique Industrielle, Passage des Déportés, 2, B-5030 Gembloux, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Eeman
authors.affiliation: Physical Chemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Gerd
authors.initials: G
authors.lastname: Olofsson
authors.affiliation: Physical Chemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Emma
authors.initials: E
authors.lastname: Sparr
authors.affiliation: Université de Liège, Gembloux Agro-Bio-Tech, Laboratoire de Biophysique Moléculaire aux Interfaces, Passage des Déportés, 2, B-5030 Gembloux, Belgium.
authors.forename: Mehmet Nail
authors.initials: MN
authors.lastname: Nasir
authors.affiliation: Physical Chemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Tommy
authors.initials: T
authors.lastname: Nylander
authors.affiliation: Université de Liège, Gembloux Agro-Bio-Tech, Laboratoire de Biophysique Moléculaire aux Interfaces, Passage des Déportés, 2, B-5030 Gembloux, Belgium. Electronic address: Magali.Deleu@ulg.ac.be.
authors.forename: Magali
authors.initials: M
authors.lastname: Deleu
mesh_terms: D002152:Calorimetry, Differential Scanning / Q000379:methods*; D006358:Hot Temperature; D055666:Lipopeptides / Q000737:chemistry / Q000378:metabolism*; D008567:Membranes, Artificial; D008823:Micelles; D012867:Skin / Q000378:metabolism*; D013997:Time Factors
chemical_list: D055666:Lipopeptides; D008567:Membranes, Artificial; D008823:Micelles; C049972:fengycin
keywords: Critical micelle concentration; Differential scanning calorimetry; Isothermal titration calorimetry; Phase transition; Temperature effect