title: PSEN1 and PSEN2 gene expression in Alzheimer's disease brain: a new approach.
abstract: Presenilin 1 (PSEN1) and presenilin 2 (PSEN2) genes encode the major component of y-secretase, which is responsible for sequential proteolytic cleavages of amyloid precursor proteins and the subsequent formation of amyloid-β peptides. 150 RNA samples from the entorhinal cortex, auditory cortex and hippocampal regions of individuals with Alzheimer's disease (AD) and controls elderly subjects were analyzed with using real-time rtPCR. There were no differences between groups for PSEN1 expression. PSEN2 was significantly downregulated in the auditory cortex of AD patients when compared to controls and when compared to other brain regions of the patients. Alteration in PSEN2 expression may be a risk factor for AD.
authors.affiliation: Faculdade de Medicina de Marília, São Paulo, Brazil.
authors.forename: Roger
authors.initials: R
authors.lastname: Delabio
authors.affiliation: Universidades do Sagrado Coração, Bauru, São Paulo, Brazil.
authors.forename: Lucas
authors.initials: L
authors.lastname: Rasmussen
authors.affiliation: Faculdade de Medicina de Marília, São Paulo, Brazil.
authors.forename: Igor
authors.initials: I
authors.lastname: Mizumoto
authors.affiliation: Faculdade de Medicina de Marília, São Paulo, Brazil.
authors.forename: Gustavo-Arruda
authors.initials: GA
authors.lastname: Viani
authors.affiliation: Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Chen
authors.affiliation: Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: João
authors.initials: J
authors.lastname: Villares
authors.affiliation: Faculdade de Medicina de Marília, São Paulo, Brazil.
authors.forename: Isabela-Bazzo
authors.initials: IB
authors.lastname: Costa
authors.affiliation: The Brain Bank - Douglas Hospital Research Centre, Montreal, QC, Canada.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Turecki
authors.affiliation: Laboratório NeoGene, Faculdade de Medicina da UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Sandra Aparecido
authors.initials: SA
authors.lastname: Linde
authors.affiliation: Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Marilia Cardoso
authors.initials: MC
authors.lastname: Smith
authors.affiliation: Faculdade de Medicina de Marília, São Paulo, Brazil Universidades do Sagrado Coração, Bauru, São Paulo, Brazil.
authors.forename: Spencer-Luiz
authors.initials: SL
authors.lastname: Payão
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000473:pathology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D019751:Intramolecular Transferases / Q000378:metabolism; D008297:Male; D053764:Presenilin-1 / Q000235:genetics* / Q000378:metabolism; D053766:Presenilin-2 / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D053764:Presenilin-1; D053766:Presenilin-2; D019751:Intramolecular Transferases; C568454:marneral synthase, Arabidopsis
keywords: Alzheimer's disease; brain; gene expression; presenilin-1; presenilin-2; γ-secretase