title: Cyclooxygenase-2 expression in pigs infected experimentally with Mycoplasma hyopneumoniae.
abstract: Porcine enzootic pneumonia, primarily caused by Mycoplasma hyopneumoniae (Mh), is a contagious disease characterized by catarrhal bronchointerstitial pneumonia. Previous studies have evaluated immunohistochemically the distribution of Mh, different cellular populations and cytokines during Mh-induced pneumonia. Cyclooxygenase (COX)-2 is overexpressed during inflammatory responses by different cell types in the lung. The aim of this study was to elucidate the possible role of COX-2 in the pathogenesis of porcine enzootic pneumonia. COX-2 protein was detected by immunohistochemistry in formalin-fixed, paraffin wax-embedded lung tissues from 10 pigs infected experimentally with Mh. Ten pigs were inoculated intranasally with Mh and killed in pairs weekly from 1 to 5 weeks post inoculation. Three Mh-free pigs were taken as controls. Bronchial and bronchiolar epithelial cells, bronchial submucosal glands and a small number of macrophages in the bronchoalveolar exudate expressed COX-2. COX-2 protein was always associated with areas of pneumonia and expression was minimal in lungs from control pigs. These results suggest that COX-2 plays a role in the pathogenesis of Mh-infection.
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, 35413 Arucas, Gran Canaria, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Andrada
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, 35413 Arucas, Gran Canaria, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Quesada-Canales
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, 35413 Arucas, Gran Canaria, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Suárez-Bonnet
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, 35413 Arucas, Gran Canaria, Spain.
authors.forename: Y
authors.initials: Y
authors.lastname: Paz-Sánchez
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, 35413 Arucas, Gran Canaria, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Espinosa de Los Monteros
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, 35413 Arucas, Gran Canaria, Spain. Electronic address: frodriguez@dmor.ulpgc.es.
authors.forename: F
authors.initials: F
authors.lastname: Rodríguez
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000032:analysis / Q000096:biosynthesis*; D007150:Immunohistochemistry; D045705:Mycoplasma hyopneumoniae; D045729:Pneumonia of Swine, Mycoplasmal / Q000201:enzymology*; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000201:enzymology* / Q000473:pathology
chemical_list: D051546:Cyclooxygenase 2
keywords: Mycoplasma hyopneumoniae; cyclooxygenase-2; pig; pneumonia