title: Establishing biological reference intervals for novel platelet parameters (immature platelet fraction, high immature platelet fraction, platelet distribution width, platelet large cell ratio, platelet-X, plateletcrit, and platelet distribution width) and their correlations among each other.
abstract: OBJECTIVE
  This study aims to establish biological reference interval for novel platelet parameters.
  
  
  METHODS
  A total of 945 healthy individuals, age ranges from 18 to 64 years (881 males and 64 females) coming for voluntary blood donation from June to August 2012 (3 months) were enrolled after exclusion of rejection criteria.
  
  
  METHODS
  The samples were assayed by running in complete blood count + reticulocyte mode on the Sysmex XE-2100 hematology analyzer and the reference interval for the population was calculated using Clinical and Laboratory Standards Institute guidelines.
  
  
  METHODS
  Tests were performed using SPSS (Statistical Product and Service Solutions, developed by IBM corporation), version 13. Student t test and pearsons correlation analysis were also used.
  
  
  RESULTS
  The normal range for various parameters was platelet count: 150-520 × 10(3)/cu mm, immature platelet fraction (IPF): 0.3-8.7%, platelet distribution width (PDW): 8.3-25.0 fL, mean platelet volume (MPV): 8.6-15.5 fL, plateletcrit (PCT): 0.15-0.62%, high immature platelet fraction (H-IPF): 0.1-2.7%, platelet large cell ratio (P-LCR): 11.9-66.9% and platelet-X (PLT-X) (ch): 11.0-22.0. Negative correlation was observed between platelet count (r = -0.468 to r = -0.531; P < 0.001) and PCT (r = -0.080 to r = -0.235; P < 0.05 to P < 0.001) with IPF, PDW, MPV, H-IPF, P-LCR, and platelet-X. IPF/H-IPF showed a positive correlation among them and also with PDW, MPV, P-LCR, platelet-X (r = +0.662 to r = +0.925; P < 0.001).
  
  
  CONCLUSIONS
  These novel platelet parameters offer newer avenues in research and clinical use. Establishing biological reference interval for different platelet parameters would help determine true high and low values and help guide treatment decisions.
authors.affiliation: Department of Pathology, Lab Medicine and Transfusion Medicine, Medanta-The Medicity Hospital, Gurgaon, Haryana, India.
authors.forename: Ritesh
authors.initials: R
authors.lastname: Sachdev
authors.forename: Aseem K
authors.initials: AK
authors.lastname: Tiwari
authors.forename: Shalini
authors.initials: S
authors.lastname: Goel
authors.forename: Vimarsh
authors.initials: V
authors.lastname: Raina
authors.forename: Monisha
authors.initials: M
authors.lastname: Sethi
mesh_terms: D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology*; D005260:Female; D006403:Hematologic Tests / Q000592:standards*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D055815:Young Adult