title: Mitigating the effects of Xuebijing injection on hematopoietic cell injury induced by total body irradiation with γ rays by decreasing reactive oxygen species levels.
abstract: Hematopoietic injury is the most common side effect of radiotherapy. However, the methods available for the mitigating of radiation injury remain limited. Xuebijing injection (XBJ) is a traditional Chinese medicine used to treat sepsis in the clinic. In this study, we investigated the effects of XBJ on the survival rate in mice with hematopoietic injury induced by γ ray ionizing radiation (IR). Mice were intraperitoneally injected with XBJ daily for seven days after total body irradiation (TBI). Our results showed that XBJ (0.4 mL/kg) significantly increased 30-day survival rates in mice exposed to 7.5 Gy TBI. This effect may be attributable to improved preservation of white blood cells (WBCs) and hematopoietic cells, given that bone marrow (BM) cells from XBJ-treated mice produced more granulocyte-macrophage colony forming units (CFU-GM) than that in the 2 Gy/TBI group. XBJ also decreased the levels of reactive oxygen species (ROS) by increasing glutathione (GSH) and superoxide dismutase (SOD) levels in serum and attenuated the increased BM cell apoptosis caused by 2 Gy/TBI. In conclusion, these findings suggest that XBJ enhances the survival rate of irradiated mice and attenuates the effects of radiation on hematopoietic injury by decreasing ROS production in BM cells, indicating that XBJ may be a promising therapeutic candidate for reducing hematopoietic radiation injury.
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China. lideguan@irm-cams.ac.cn.
authors.forename: Deguan
authors.initials: D
authors.lastname: Li
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China. lulu@irm-cams.ac.cn.
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China. zhangjunling@irm-cams.ac.cn.
authors.forename: Junling
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China. wangxiaochun@irm-cams.ac.cn.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China. xyhandy@163.com.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China. why999@irm-cams.ac.cn.
authors.forename: Hongying
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Hematology and Oncology, the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. yxdtcm@sohu.com.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Hematology and Oncology, the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. shzhx0604@163.com.
authors.forename: Zhexin
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of Hematology and Oncology, Tianjin First Central Hospital, Tianjin 300192, China. zmfzmf@hotmail.com.
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China. fansaijun@irm-cams.ac.cn.
authors.forename: Saijun
authors.initials: S
authors.lastname: Fan
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China. aiminmeng@irm-cams.ac.cn.
authors.forename: Aimin
authors.initials: A
authors.lastname: Meng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005720:Gamma Rays; D005978:Glutathione / Q000097:blood; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood; D014916:Whole-Body Irradiation
chemical_list: D004365:Drugs, Chinese Herbal; D017382:Reactive Oxygen Species; C549376:Xuebijing; D013482:Superoxide Dismutase; D005978:Glutathione