title: The β-SiC nanowires (~100 nm) induce apoptosis via oxidative stress in mouse osteoblastic cell line MC3T3-E1.
abstract: Silicon carbide (SiC), a compound of silicon and carbon, with chemical formula SiC, the beta modification ( β-SiC), with a zinc blende crystal structure (similar to diamond), is formed at temperature below 1700°C. β-SiC will be the most suitable ceramic material for the future hard tissue replacement, such as bone and tooth. The in vitro cytotoxicity of β-SiC nanowires was investigated for the first time. Our results indicated that 100 nm long SiC nanowires could significantly induce the apoptosis in MC3T3-E1 cells, compared with 100 μm long SiC nanowires. And 100 nm long SiC nanowires increased oxidative stress in MC3T3-E1 cells, as determined by the concentrations of MDA (as a marker of lipid peroxidation) and 8-OHdG (indicator of oxidative DNA damage). Moreover, transmission electron microscopy (TEM) was performed to evaluate the morphological changes of MC3T3-E1 cells. After treatment with 100 nm long SiC nanowires, the mitochondria were swelled and disintegrated, and the production of ATP and the total oxygen uptake were also decreased significantly. Therefore, β-SiC nanowires may have limitations as medical material.
authors: School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China ; Department of Prosthodontics, School of Stomatology, Harbin Medical University, Harbin 150001, China.
authors: Weili
authors: W
authors: Xie
authors: Department of Prosthodontics, School of Stomatology, Harbin Medical University, Harbin 150001, China.
authors: Qi
authors: Q
authors: Xie
authors: Department of Prosthodontics, School of Stomatology, Harbin Medical University, Harbin 150001, China.
authors: Meishan
authors: M
authors: Jin
authors: School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China.
authors: Xiaoxiao
authors: X
authors: Huang
authors: School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China.
authors: Xiaodong
authors: X
authors: Zhang
authors: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Zhengkai
authors: Z
authors: Shao
authors: School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China ; School of Materials Science and Engineering, Harbin Institute of Technology at Weihai, Weihai 264209, China.
authors: Guangwu
authors: G
authors: Wen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D017554:Carbon Compounds, Inorganic / Q000009:adverse effects* / Q000494:pharmacology; D002460:Cell Line; D015227:Lipid Peroxidation / Q000187:drug effects*; D008422:Materials Testing / Q000379:methods; D051379:Mice; D053770:Nanowires / Q000009:adverse effects*; D010006:Osteoblasts / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D017655:Silicon Compounds / Q000009:adverse effects* / Q000494:pharmacology
chemical_list: D017554:Carbon Compounds, Inorganic; D017655:Silicon Compounds; C022088:silicon carbide