title: Research on a novel poly (vinyl alcohol)/lysine/vanillin wound dressing: Biocompatibility, bioactivity and antimicrobial activity.
abstract: Burn wound dressings have played significant roles in daily clinical practice. An "ideal" burn wound dressing is non-adhesion, absorbency and antimicrobial activity. However, such a dressing is currently not available. A novel composite hydrogel was based on poly (vinyl alcohol) (PVA) containing lysine (Lys) and vanillin (V) using freezing-thawing method. The properties of this hydrogel were characterized by environmental scanning electron microscope (ESEM), attenuated total reflectance fourier transform infrared spectroscopy (ATR-FTIR), tensile testing, differential scanning calorimetry (DSC) and water vapour transmission rate (WVTR). Then the antibacterial activity of PVA/Lys/V composite hydrogel was examined by bacteriolytic plate. In vivo experiment, a burn rat model was used to evaluate the histological analysis of this hydrogel. In results, the Schiff base formed in the three-phase system. It improved the tensile strength and crystallization of the PVA/Lys/V composite hydrogel. Meanwhile, this hydrogel showed excellent bactericidal activities to both gram-negative bacteria (E. coli) and gram-positive bacteria (S. aureus) due to the Schiff base. And the antibacterial activity toward gram-negative bacteria was better than another. On Day 7, 95-100% of the surface areas of PVA/Lys/V composite treated burns were covered with regenerating epidermis. And the new tissue and capillary vessel formed around the wounds after treatment. Therefore, it is suggested that treatment with PVA/Lys/V composite hydrogel will be effective also in patients with burns and other skin wounds.
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors: Gang
authors: G
authors: Zhou
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors: Ruhan
authors: R
authors: A
authors: The Airforce Armament Academy, Beijing 100191, China.
authors: Heng
authors: H
authors: Ge
authors: Nuclear and Radiation Safety Center, Ministry of Environmental Protection, Beijing 100082, China.
authors: Lin
authors: L
authors: Wang
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors: Meili
authors: M
authors: Liu
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors: Binbin
authors: B
authors: Wang
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors: Haisheng
authors: H
authors: Su
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors: Ming
authors: M
authors: Yan
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors: Yuan
authors: Y
authors: Xi
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China. Electronic address: yubofan@buaa.edu.cn.
authors: Yubo
authors: Y
authors: Fan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001458:Bandages; D001547:Benzaldehydes / Q000494:pharmacology*; D002056:Burns / Q000628:therapy*; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000187:drug effects; D020100:Hydrogels / Q000494:pharmacology*; D008239:Lysine / Q000494:pharmacology*; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D051381:Rats; D013211:Staphylococcus aureus / Q000187:drug effects; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001547:Benzaldehydes; D020100:Hydrogels; D011142:Polyvinyl Alcohol; C100058:vanillin; D008239:Lysine
keywords: Lysine; Poly(vinyl alcohol); Schiff base; Vanillin; Wound healing