title: Androglobin knockdown inhibits growth of glioma cell lines.
abstract: Globin family was famous for oxygen supply function of its members such as hemoglobin and myoglobin. With the progress of research, several members of this protein family have been proven to play roles in tumors including glioma. Androglobin (ADGB) is a recently identified member of globin family with very few studies about its function. In the present study, we show that ADGB plays an oncogene role in glioma. Lentiviral vector mediated ADGB knockdown inhibited the proliferation of glioma cell lines determined by MTT assay and colony formation assay. ADGB knockdown also increased the apoptosis of glioma cell line U251 assessed by flow cytometry. In addition, western blot showed that ADGB knockdown altered levels of several proteins related to proliferation, survival or apoptosis in U251 cells. These findings suggest ADGB is involved in the progression of glioma in vitro.
authors: The 2nd Department of Orthopaedics, 117 Hospital of PLA Hangzhou, China.
authors: Bo
authors: B
authors: Huang
authors: The 2nd Department of Orthopaedics, 117 Hospital of PLA Hangzhou, China.
authors: Yi-Sheng
authors: YS
authors: Lu
authors: Yi Ji Shan Hospital of Wan Nan Medical College Anhui, China.
authors: Xia
authors: X
authors: Li
authors: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
authors: Zhi-Chuan
authors: ZC
authors: Zhu
authors: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
authors: Kui
authors: K
authors: Li
authors: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
authors: Ji-Wei
authors: JW
authors: Liu
authors: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
authors: Jing
authors: J
authors: Zheng
authors: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
authors: Ze-Lan
authors: ZL
authors: Hu
mesh_terms: D017209:Apoptosis; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005914:Globins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
chemical_list: C000595297:ADGB protein, human; D002148:Calmodulin-Binding Proteins; D034741:RNA, Small Interfering; D005914:Globins
keywords: Androglobin; apoptosis; glioma; proliferation