title: Association of myeloid cells of triggering receptor-1 with left ventricular systolic dysfunction in BALB/c mice with sepsis.
abstract: OBJECTIVE
  To investigate the correlation between TREM-1 and LPS-induced left ventricular systolic dysfunction in BALB/c mice.
  
  
  METHODS
  Male BALB/c mice were randomly divided into 3 groups: LPS, LPS/TREM-1, and control groups which were injected intraperitoneally with 25 mg/kg LPS, 5 μg TREM-1mAb 1 h after LPS challenge, and sterilized normal saline, respectively. Left ventricular systolic function was monitored by echocardiography at 6 h, 12 h, and 24 h. Meanwhile, TNF- α , IL-1 β , and sTREM-1 in serum and myocardium were determined by ELISA or real-time PCR; at last left ventricles were taken for light microscopy examination.
  
  
  RESULTS
  FS and EF in LPS/mAbTREM-1 group, significantly declined compared with LPS and control group at 12 h, were accompanied with a markedly increase in serum IL-1 β (at 6 h) and sTREM-1 (at 12 h and 24 h) expression. Myocardium TNF- α (at 6 h and 24 h) and sTREM-1 (at 6 h) were significantly higher in LPS/mAbTrem-1-treated mice than in time-matched LPS-treated mice; meanwhile myocardium TNF- α mRNA were markedly increased in comparison with LPS-treated or saline-treated mice at 24 h. Besides, mAbTREM-1 aggravated LPS-induced myocardial damage was observed.
  
  
  CONCLUSIONS
  Our results suggest that TREM-1 is significantly associated with LPS-induced left ventricular systolic dysfunction in BALB/c mice.
authors: Department of Critical Care Medicine, Affiliated Hospital of Guangdong Medical College, No. 57 Southern Renmin Avenue, Zhanjiang, Guangdong 524023, China.
authors: Gaosheng
authors: 0000-0001-5268-1491
authors: G
authors: Zhou
authors: Department of Critical Care Medicine, Affiliated Hospital of Guangdong Medical College, No. 57 Southern Renmin Avenue, Zhanjiang, Guangdong 524023, China.
authors: Lijun
authors: L
authors: Ye
authors: Department of Critical Care Medicine, Affiliated Hospital of Guangdong Medical College, No. 57 Southern Renmin Avenue, Zhanjiang, Guangdong 524023, China.
authors: Liang
authors: L
authors: Zhang
authors: Department of Critical Care Medicine, Affiliated Hospital of Guangdong Medical College, No. 57 Southern Renmin Avenue, Zhanjiang, Guangdong 524023, China.
authors: Liangqing
authors: L
authors: Zhang
authors: Department of Critical Care Medicine, Affiliated Hospital of Guangdong Medical College, No. 57 Southern Renmin Avenue, Zhanjiang, Guangdong 524023, China.
authors: Yuanli
authors: Y
authors: Zhang
authors: Department of Critical Care Medicine, Affiliated Hospital of Guangdong Medical College, No. 57 Southern Renmin Avenue, Zhanjiang, Guangdong 524023, China.
authors: Liehua
authors: L
authors: Deng
authors: Department of Critical Care Medicine, Affiliated Hospital of Guangdong Medical College, No. 57 Southern Renmin Avenue, Zhanjiang, Guangdong 524023, China.
authors: Huaguo
authors: H
authors: Yao
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood; D004452:Echocardiography; D007249:Inflammation / Q000097:blood; D008070:Lipopolysaccharides / Q000737:chemistry*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D022423:Myeloid Cells / Q000166:cytology*; D009206:Myocardium / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism*; D018805:Sepsis / Q000097:blood*; D013997:Time Factors; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D018487:Ventricular Dysfunction, Left / Q000097:blood*
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C517262:TREM1 protein, mouse; D000073871:Triggering Receptor Expressed on Myeloid Cells-1