title: Interaction of S100A13 with C2 domain of receptor for advanced glycation end products (RAGE).
abstract: S100A13 is involved in several key biological functions like angiogenesis, tumor formation and cell apoptosis. It is a homodimeric protein that belongs to the S100 protein family. S100A13 is co-expressed with acidic fibroblast growth factor (FGF1) and interleukin-1α which are key angiogenesis inducers. The S100 proteins have been shown to be involved in several cellular functions such as calcium homeostasis, cell growth and differentiation dynamic of cytoskeleton. Its biological functions are mainly mediated through the receptor for advanced glycation end products (RAGE) signaling. RAGE is involved in inflammatory processes and is associated with diabetic complications, tumor outgrowth, and neurodegenerative disorders. RAGE induces cellular signaling upon binding of different ligands, such as S100 proteins, glycated proteins, and HMGB1. RAGE signaling is complex, and it depends on the cell type and concentration of the ligand. Molecular level interactions of RAGE and S100 proteins are useful to understand the RAGE signaling diversity. In this report we focus on the molecular level interactions of S100A13 and RAGE C2 domain. The binding between RAGE C2 and S100A13 is moderately strong (Kd~1.3μM). We have solved the solution structure of the S100A13-RAGE C2 complex and pronounce the interface regions in S100A13-RAGE C2 complex which are helpful for drug development of RAGE induced diseases.
authors: Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan. Electronic address: sandya10@gmail.com.
authors: Sandhya G
authors: SG
authors: Rani
authors: Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan. Electronic address: mohansepuri@gmail.com.
authors: Krishna Mohan
authors: KM
authors: Sepuru
authors: Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan. Electronic address: cyu.nthu@gmail.com.
authors: Chin
authors: C
authors: Yu
mesh_terms: D002151:Calorimetry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D011485:Protein Binding; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D009418:S100 Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D009418:S100 Proteins; C511523:S100A13 protein, human
keywords: Isothermal titration calorimetry; NMR spectroscopy; RAGE dimerization; Receptor for advanced glycation end products; S100A13