title: Efficiency of MY09/11 consensus PCR in the detection of multiple HPV infections.
abstract: Human papillomavirus (HPV) DNA testing has become an important component of cervical cancer screening programs. In this study, we aimed to evaluate the efficiency of MY09/11 consensus polymerase chain reaction (PCR) for the detection of multiple HPV infections. For this purpose, MY09/11 PCR was compared to an original TaqMan-based type-specific real-time PCR assay, which can detect 20 different HPV types. Of the 654 samples, 34.1% (223/654) were HPV DNA positive according to at least one method. The relative sensitivities of MY09/11 PCR and type-specific PCR were 80.7% (180/223) and 97.8% (218/223), respectively. In all, 352 different HPV isolates (66 low-risk and 286 high-risk or probable high-risk types) were identified in 218 samples, but 5 samples, which were positive by consensus PCR only, could not be genotyped. The distribution of the 286 high-risk or probable high-risk HPVs were as follows: 24.5% HPV-16, 8.4% HPV-52, 7.7% HPV-51, 6.3% HPV-39, 6.3% HPV-82, 5.6% HPV-35, 5.6% HPV-58, 5.6% HPV-66, 5.2% HPV-18, 5.2% HPV-68, and 19.6% the other 8 types. A single HPV type was detected in 57.3% (125/218) of the genotyped samples, and multiple HPV types were found in the remaining 42.7% (93/218). The false-negative rates of MY09/11 PCR were found to be 17.4% in single infections, 23.3% in multiple infections, and 34.6% in multiple infections that contained 3 or more HPV types, with the condition that the low-risk types HPV-6 and HPV-11 be considered as a monotype. These data suggest that broad-range PCR assays may lead to significant data loss and that type-specific PCR assays can provide accurate and reliable results during cervical cancer screening.
authors: Division of Medical Virology, Department of Medical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey. Electronic address: fsvirol@gmail.com.
authors: Fatih
authors: F
authors: Şahiner
authors: Division of Medical Virology, Department of Medical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey. Electronic address: akubar@hotmail.com.
authors: Ayhan
authors: A
authors: Kubar
authors: Department of Medical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey. Electronic address: rgumral@gata.edu.tr.
authors: Ramazan
authors: R
authors: Gümral
authors: Ankara Atatürk Training and Research Hospital, Yıldırım Beyazıt University, Ankara, Turkey. Electronic address: mardic@yahoo.com.
authors: Medine
authors: M
authors: Ardıç
authors: Department of Medical Pathology, Gulhane Military Medical Academy, Ankara, Turkey. Electronic address: nyigitsum@gmail.com.
authors: Nuri
authors: N
authors: Yiğit
authors: Division of Medical Virology, Department of Medical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey. Electronic address: ksener@gata.edu.tr.
authors: Kenan
authors: K
authors: Şener
authors: Department of Obstetrics and Gynecology, Gulhane Military Medical Academy, Ankara, Turkey. Electronic address: mdede@gata.edu.tr.
authors: Murat
authors: M
authors: Dede
authors: Department of Medical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey. Electronic address: myapar@gata.edu.tr.
authors: Mehmet
authors: M
authors: Yapar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002584:Cervix Uteri / Q000821:virology; D005260:Female; D061809:Human Papillomavirus DNA Tests / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D016133:Polymerase Chain Reaction; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000821:virology; D055815:Young Adult
keywords: Cervical smear; Genotyping; Mismatches; Type distribution