title: Investigating the structure of the factor B vWF-A domain/CD55 protein-protein complex using DEER spectroscopy: successes and pitfalls.
abstract: The electron paramagnetic resonance technique of double electron-electron resonance (DEER) was used to measure nanometre-scale distances between nitroxide spin labels attached to the complement regulatory protein CD55 (also known as decay accelerating factor) and the von Willebrand factor A (vWF-A) domain of factor B. Following a thorough assessment of the quality of the data, distances obtained from good-quality measurements are compared to predicted distances from a previously hypothesised model for the complex and are found to be incompatible. The success of using these distances as restraints in multi-body docking routines is presented critically.
authors: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK ; EaStCHEM School of Chemistry, University of Edinburgh, Edinburgh, UK.
authors: Janet E
authors: JE
authors: Lovett
authors: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
authors: Rachel J M
authors: RJ
authors: Abbott
authors: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
authors: Pietro
authors: P
authors: Roversi
authors: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
authors: Steven
authors: S
authors: Johnson
authors: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK ; Centre for Advanced Electron Spin Resonance, Inorganic Chemistry Laboratory, University of Oxford, Oxford, UK.
authors: Joseph J E
authors: JJ
authors: Caesar
authors: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK ; Centre for Advanced Electron Spin Resonance, Inorganic Chemistry Laboratory, University of Oxford, Oxford, UK.
authors: Marianna
authors: M
authors: Doria
authors: Laboratory for Physical Chemistry, ETH Zürich, Zürich, Switzerland.
authors: Gunnar
authors: G
authors: Jeschke
authors: Centre for Advanced Electron Spin Resonance, Inorganic Chemistry Laboratory, University of Oxford, Oxford, UK.
authors: Christiane R
authors: CR
authors: Timmel
authors: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
authors: Susan M
authors: SM
authors: Lea
keywords: CD55; DEER; decay acceleration; distance restraints; vWF-A