title: DNA methylation of HOXA10 in eutopic and ectopic endometrium.
abstract: OBJECTIVE
  Does the methylation status of the promoter region of the HOXA10 gene differ in eutopic and ectopic endometrium?
  
  
  CONCLUSIONS
  The eutopic endometrium in women with endometriosis is significantly more methylated when compared with controls.
  
  
  BACKGROUND
  Expression of the HOXA10 gene, which is important for successful implantation, is reduced in women affected by endometriosis.
  
  
  METHODS
  A pilot study was carried out including 18 women admitted for surgery for endometriosis-related pain (cases) and 12 women admitted for surgery because of non-endometriotic disease (control). Sample collection and analysis were performed between November 2010 and July 2013.
  
  
  METHODS
  Endometrial tissue (eutopic and ectopic) underwent sodium bisulfite DNA modification, PCR amplification of two regions of the HOXA10 promoter and pyrosequencing analysis.
  
  
  RESULTS
  The eutopic endometrium of women with endometriosis was significantly more methylated compared with endometrium from the control group (sequence 1: 8.68% in cases and 6.25% in the control group: P = 0.037, sequence 2: 11.89% in cases and 9.25% in the control group: P = 0.032). The eutopic endometrium was significantly more methylated than the ectopic tissue in patients with endometriosis (mean difference -3.6 sequence 1: P = 0.001 and -6.0 sequence 2: P = 0.0001).
  
  
  CONCLUSIONS
  The study had a limited sample size and the fertility status of the majority of patients in our study was unknown.
  
  
  CONCLUSIONS
  Our data regarding methylation state of the ectopic tissues contribute to a better etiopathologic understanding of endometriosis.
  
  
  BACKGROUND
  No external funding was either sought or obtained for this study. The authors have no conflicts of interests to declare.
authors: ISPO Cancer Prevention and Research Institute, Via Cosimo Il Vecchio 2, Florence 50139, Italy Department of Women's and Children's Health, Division of Obstetrics and Gynaecology, Karolinska Institutet/Karolinska University Hospital, Solna 17176, Sweden karinlouiseandersson@gmail.com.
authors: K L
authors: KL
authors: Andersson
authors: Department of Biomedical, Experimental and Clinical Sciences, Gynaecology and Obstetrics Unit, University of Florence, Florence 50134, Italy.
authors: C
authors: C
authors: Bussani
authors: Department of Biomedical, Experimental and Clinical Sciences, Gynaecology and Obstetrics Unit, University of Florence, Florence 50134, Italy.
authors: M
authors: M
authors: Fambrini
authors: Department of Biomedical, Experimental and Clinical Sciences, Gynaecology and Obstetrics Unit, University of Florence, Florence 50134, Italy.
authors: V
authors: V
authors: Polverino
authors: Department of Human Pathology and Oncology, University of Florence, Florence 50134, Italy.
authors: G L
authors: GL
authors: Taddei
authors: Department of Women's and Children's Health, Division of Obstetrics and Gynaecology, Karolinska Institutet/Karolinska University Hospital, Solna 17176, Sweden.
authors: K
authors: K
authors: Gemzell-Danielsson
authors: Department of Biomedical, Experimental and Clinical Sciences, Gynaecology and Obstetrics Unit, University of Florence, Florence 50134, Italy.
authors: G
authors: G
authors: Scarselli
mesh_terms: D000328:Adult; D019175:DNA Methylation; D004715:Endometriosis / Q000235:genetics*; D004717:Endometrium / Q000378:metabolism / Q000473:pathology*; D005260:Female; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010865:Pilot Projects
chemical_list: D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; C069634:HOXA10 protein, human
keywords: DNA methylation; HOXA10 gene; endometriosis