title: A sheath-less combined optical and impedance micro-cytometer.
abstract: We describe a sheath-less micro-cytometer that measures four different parameters, namely fluorescence, large angle side scatter and dual frequency electrical impedance (electrical volume and opacity). The cytometer was benchmarked using both size and fluorescent bead standards and demonstrates excellent size accuracy (CVs ≤ 2.1%), sensitivity and dynamic range (3.5 orders of magnitude) at sample flow rates of 80 μL per minute. The cytometer was evaluated by analysing human blood, and a four part differential leukocyte assay for accurate CD4+ T-cell enumeration was demonstrated. The integration of impedance, fluorescence and side scatter into a single miniature cytometer platform provides the core information content of a classical cytometer in a highly compact, simple, portable and low cost format.
authors: Faculty of Physical Sciences and Engineering, and Institute for Life Sciences, University of Southampton, Southampton, Hampshire SO17 1BJ, UK. hm@ecs.soton.ac.uk.
authors: Daniel
authors: D
authors: Spencer
authors: Gregor
authors: G
authors: Elliott
authors: Hywel
authors: H
authors: Morgan
mesh_terms: D001773:Blood Cells / Q000166:cytology; D017097:Electric Impedance; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D012542:Scattering, Radiation