title: Stability and folding of amphibian ribonuclease A superfamily members in comparison with mammalian homologues.
abstract: Comparative studies on homologous proteins can provide knowledge on how limited changes in the primary structure find their expression in large effects on catalytic activity, stability or the folding behavior. For more than half a century, members of the ribonuclease A superfamily have been the subject of a myriad of studies on protein folding and stability. Both the unfolding and refolding kinetics as well as the structure of several folding intermediates of ribonuclease A have been characterized in detail. Moreover, the RNA-degrading activity of these enzymes provides a basis for their cytotoxicity, which renders them potential tumor therapeutics. Because amphibian ribonuclease A homologues evade the human ribonuclease inhibitor, they emerged as particularly promising candidates. Interestingly, the amphibian ribonuclease A homologues investigated to date are more stable than the mammalian homologues. Nevertheless, despite the generation of numerous genetically engineered variants, knowledge of the folding of amphibian ribonuclease A homologues remains rather limited. An exception is onconase, a ribonuclease A homologue from Rana pipiens, which has been characterized in detail. This review summarizes the data on the unfolding and refolding kinetics and pathways, as well on the stability of amphibian ribonuclease A homologues compared with those of ribonuclease A, the best known member of this superfamily.
authors: Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Germany.
authors: Ulrich
authors: U
authors: Arnold
mesh_terms: D029845:Amphibian Proteins / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D004795:Enzyme Stability; D006801:Humans; D007700:Kinetics; D010084:Oxidation-Reduction; D017510:Protein Folding; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D044366:Transition Temperature
chemical_list: D029845:Amphibian Proteins; D012259:Ribonuclease, Pancreatic
keywords: cytotoxicity; disulfide bond; folding pathway; homologues; intermediate; kinetics; onconase; protein engineering; ribonuclease A superfamily; stability