title: Dose-Response Relationship Between Orally Administered Ammonium Perchlorate and Urine Perchlorate Concentrations in Rats: Possible Biomarker to Quantify Environmental Ammonium Perchlorate Exposure on Thyroid Homeostasis.
abstract: To evaluate the feasibility of urine perchlorate as a biomarker of ammonium perchlorate (AP) exposure and to explore the correlation between the thyroid function indicators and the perchlorate concentrations, a sensitive and selective ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) method was developed to detect perchlorate in urine samples. Rats were orally administrated with different doses of perchlorate. Serum free thyroxine (FT4), free triiodothyronine (FT3), and thyroid-stimulating hormone (TSH) were determined by radioimmunoassays. The results showed that a dose of AP up to 520 mg kg(-1) body weight induced a significant increase of TSH, with a decrease of FT4. Particularly, the levels of urine perchlorate increased dose-dependently on AP exposure from drinking water. The findings highlighted that urine perchlorate may be a useful biomarker for AP environmental exposure.
authors: a Institute of Biomedicine, Taihe Hospital , Hubei University of Medicine , Shiyan , People's Republic of China.
authors: Hong Xia
authors: HX
authors: Chen
authors: Miao Hong
authors: MH
authors: Ding
authors: Yong Gan
authors: YG
authors: Li
authors: Qin
authors: Q
authors: Liu
authors: Kai Liang
authors: KL
authors: Peng
mesh_terms: D000284:Administration, Oral; D000818:Animals; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure / Q000032:analysis*; D006706:Homeostasis / Q000187:drug effects; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010472:Perchlorates / Q000008:administration & dosage* / Q000633:toxicity / Q000652:urine*; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage* / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013961:Thyroid Gland / Q000187:drug effects*; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
chemical_list: D015415:Biomarkers; D010472:Perchlorates; D000644:Quaternary Ammonium Compounds; D014284:Triiodothyronine; D013972:Thyrotropin; D013974:Thyroxine; C494474:perchlorate; C053506:ammonium perchlorate
keywords: ammonium perchlorate; biomarker; thyroid hormone; urine perchlorate