title: The influence of shc proteins on the whole body energetic response to calorie restriction initiated in 3-month-old mice.
abstract: There is increasing evidence that Shc proteins play a role in energy metabolism, and we have previously reported that knockdown of Shc proteins influences the energetic response to acute (3 days) calorie restriction (CR) in 18-month-old mice. Whether Shc proteins play a role in the metabolic response to CR in younger mice has yet to be elucidated. Hence, we sought to determine the impact of 3 days and longer term (2 months) CR on energy expenditure (EE) and respiratory quotient (RQ) in 3 month-old Shc knockout (ShcKO) and wild-type (WT) mice. ShcKO mice decreased (P < 0.001) EE normalized for body weight (EEBW) by 3 days of CR, while no such change was observed in WT animals. However, both ShcKO and WT mice decreased (P < 0.001) EEBW at 2 months of CR and there were no differences in body weight between the ShcKO and WT mice at either 3 days or 2 months of CR. Consistent with increased fatty acid oxidation, only ShcKO mice maintained decreased (P < 0.001) 24 h RQ through 2 months of CR, suggesting that they were able to maintain increased fatty acid oxidation for a longer period of time than WT mice. These results indicate that Shc proteins may contribute to some of the acute energetic responses to CR.
authors: VM Molecular Biosciences, University of California, Davis, CA 95616, USA ; Cancer Center Division, University of Arizona, Tucson, AZ 85724, USA.
authors: Jennifer H
authors: JH
authors: Stern
authors: Department of Public Health Sciences, University of California, Davis, CA 95616, USA.
authors: Kyoungmi
authors: 0000-0002-8661-7508
authors: K
authors: Kim
authors: VM Molecular Biosciences, University of California, Davis, CA 95616, USA.
authors: Jon J
authors: JJ
authors: Ramsey