title: Polymorphism in microRNA-196a2 contributes to the risk of cardiovascular disease in type 2 diabetes patients.
abstract: OBJECTIVE
  To investigate the effect of the microRNA-196a2 gene polymorphism (rs11614913) on risk of cardiovascular disease in type 2 diabetes patients.
  
  
  METHODS
  We examined 920 patients with diabetes and 834 healthy controls. All subjects were genotyped for the miRNA-196a2 SNP by polymerase chain reaction (PCR) and restriction analysis.
  
  
  RESULTS
  The genotype distribution among controls and patients was in Hardy-Weinberg equilibrium (p=0.227 and 0.308, respectively). The frequency of the T allele was lower in patients than in controls (p=0.044). The odds ratio 0.66 (95% CI 0.54-0.79) suggests an association of the T allele with decreased risk of T2DM. For the main purpose of the study, T2DM patients were stratified into patients with CVD and those without it. The T allele and TT genotype were significantly more frequent in patients with CVD compared to those without CVD (p=0.013, p<0.001, respectively). The odds ratio for the T allele in the CVD+subgroup vs. CVD- was 1.76 (1.35-2.30), p<0.0001, mostly due to the overrepresentation of TT homozygotes. The highest risk of development of CVD was observed in the additive model for TT homozygotes (OR 3.33, 95% CI 2.05-5.42, p<0.0001).
  
  
  CONCLUSIONS
  Our findings suggest that miRNA-196a2 T/C polymorphism (rs11614913) is associated with an increased risk of CVD in type 2 diabetes patients. This provides further insights on pathogenesis of cardiovascular disease in type 2 diabetes patients.
authors: Department of Nephrology, Medical University of Lublin, Lublin, Poland. Electronic address: monika.buraczynska@umlub.pl.
authors: Monika
authors: M
authors: Buraczynska
authors: Department of Nephrology, Medical University of Lublin, Lublin, Poland.
authors: Pawel
authors: P
authors: Zukowski
authors: Department of Cardiology, Medical University of Lublin, Lublin, Poland.
authors: Piotr
authors: P
authors: Wacinski
authors: Department of Nephrology, Medical University of Lublin, Lublin, Poland.
authors: Katarzyna
authors: K
authors: Ksiazek
authors: Department of Nephrology, Medical University of Lublin, Lublin, Poland.
authors: Wojciech
authors: W
authors: Zaluska
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002318:Cardiovascular Diseases / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: C542543:MIRN196 microRNA, human; D035683:MicroRNAs
keywords: Cardiovascular disease; Genetics; Single nucleotide polymorphism; Type 2 diabetes; miRNAs