title: Effect of choline kinase inhibitor hexadecyltrimethylammonium bromide on Plasmodium falciparum gene expression.
abstract: Investigations on the fundamental of malaria parasite biology, such as invasion, growth cycle, metabolism and cell signalling have uncovered a number of potential antimalarial drug targets, including choline kinase, a key enzyme involved in the synthesis of phosphatidylcholine, an important component in parasite membrane compartment. The effect on gene expression of Plasmodium falciparum K1 strain following 72 hours exposure to 2 microM (IC50 concentration) of the choline kinase inhibitor, hexadecyltrimethylammonium bromide (HDTAB) was evaluated by DNA microarray analysis. Genes important in P. falciparum intraerythrocytic life cycle, such as invasion, cytoadherance and growth were among those affected by at least 2-fold changes in their expression levels compared with non HDTAB-treated control.
authors: Mohd Ridzuan Mohd Abd
authors: MR
authors: Razak
authors: Noor Rain
authors: NR
authors: Abdullah
authors: Rohaya
authors: R
authors: Chomel
authors: Rosilawati
authors: R
authors: Muhamad
authors: Zakiah
authors: Z
authors: Ismail
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology*; D002797:Choline Kinase / Q000037:antagonists & inhibitors; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics*; D040081:Protein Array Analysis; D015800:Protozoan Proteins / Q000235:genetics
chemical_list: D000981:Antiprotozoal Agents; D002593:Cetrimonium Compounds; D015800:Protozoan Proteins; D002797:Choline Kinase; D000077286:Cetrimonium