title: Characteristics of KPC-2-producing Klebsiella pneumoniae (ST258) clinical isolates from outbreaks in 2 Mexican medical centers.
abstract: The KPC-producing Klebsiella pneumoniae sequence type 258 (ST258) is an important pathogen widely spread in nosocomial infections. In this study, we identified the KPC-2-producing K. pneumoniae clinical isolates of 2 unrelated outbreaks that corresponded to pandemic strain ST258. The isolates showed high resistance to cephalosporins, carbapenems, quinolones, and colistin. The KPC-2-producing K. pneumoniae isolates were compared to the previously studied KPC-3-producing K. pneumoniae isolates from an outbreak in Mexico; they showed an unrelated pulsed-field gel electrophoresis fingerprinting pattern and a different plasmid profile. The KPC-2 carbapenemase gene was identified in two 230- and 270-kb non-conjugative plasmids; however, 1 isolate transferred the KPC-2 gene onto an 80-kb plasmid. These findings endorse the need of carrying out a continuous molecular epidemiological surveillance of carbapenem-resistant isolates in hospitals in Mexico.
authors: Departamento de Diagnóstico Epidemiológico, Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
authors: Ulises
authors: U
authors: Garza-Ramos
authors: Departamento de Diagnóstico Epidemiológico, Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
authors: Humberto
authors: H
authors: Barrios
authors: Departamento de Diagnóstico Epidemiológico, Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
authors: Fernando
authors: F
authors: Reyna-Flores
authors: Departamento de Diagnóstico Epidemiológico, Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
authors: Alejandro
authors: A
authors: Sánchez-Pérez
authors: Departamento de Diagnóstico Epidemiológico, Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
authors: Elsa
authors: E
authors: Tamayo-Legorreta
authors: Hospital Regional 1(o) Octubre ISSSTE, México, DF, Mexico.
authors: Alvaro
authors: A
authors: Ibarra-Pacheco
authors: Laboratorio de Vigilancia Epidemiológica, ISSSTE, México, DF, Mexico.
authors: Juana
authors: J
authors: Salazar-Salinas
authors: Departamento de Vigilancia y Control Epidemiológico del ISSSTE, México, DF, Mexico.
authors: Ricardo
authors: R
authors: Núñez-Ceballos
authors: Departamento de Diagnóstico Epidemiológico, Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico. Electronic address: jsilva@insp.mx.
authors: Jesus
authors: J
authors: Silva-Sanchez
mesh_terms: D000046:Academic Medical Centers; D003428:Cross Infection; D004196:Disease Outbreaks; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008800:Mexico / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: C491478:beta-lactamase KPC-2; D001618:beta-Lactamases
keywords: Carbapenemase; Enterobacteria; Imipenem