title: [Cloning and gene expression of acetyl-CoA C-acetyl transferase gene (AsAACT) from Aquilaria sinensis].
abstract: OBJECTIVE
  This study aimed to clone the acetyl-CoA C-acetyl transferase (AACT) gene from Aquilaria sinensis and analyze the bioinformatics and expression of the gene.
  
  
  METHODS
  One unique sequence containing partly AACT gene sequence was discovered in our previous transcriptome dataset of A. sinensis. AACT gene was cloned by RT-PCR and RACE strategy with the template of RNA extracted from A. sinensis stem. The bioinformatic analysis of this gene and its corresponding protein was performed. The AsAACT expression in calli was analyzed with GADPH gene as an internal control gene in wounded condition by qRT-PCR technique.
  
  
  RESULTS
  One unique sequence of AACT, named as AsAACT, was cloned from A. sinensis. The full length of AsAACT cDNA was containing a 1 236 bp ORF that encoded 411 amino acids. The result of qRT-PCR displayed that the highest expression level was at 4 h. which indicated that it was possibly involved in early-stage response to wound.
  
  
  CONCLUSIONS
  Cloning and analyzing AsAACT gene from A. sinensis provided basic information for study the function and expression regulation of AsAACT in terpenoid biosynthesis.
authors: Juan
authors: J
authors: Liu
authors: Yan-Hong
authors: YH
authors: Xu
authors: Yong
authors: Y
authors: Yang
authors: Liang
authors: L
authors: Liang
authors: Xiao-Min
authors: XM
authors: Han
authors: Zhi-Hui
authors: ZH
authors: Gao
authors: Zheng
authors: Z
authors: Zhang
authors: Yun
authors: Y
authors: Yang
authors: Jian-He
authors: JH
authors: Wei
mesh_terms: D000101:Acetyl-CoA C-Acetyltransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D029645:Thymelaeaceae / Q000201:enzymology* / Q000235:genetics*
chemical_list: D000101:Acetyl-CoA C-Acetyltransferase