title: Identification of water-conditioned Pseudomonas aeruginosa by Raman microspectroscopy on a single cell level.
abstract: The identification of Pseudomonas aeruginosa from samples of bottled natural mineral water by the analysis of subcultures is time consuming and other species of the authentic Pseudomonas group can be a problem. Therefore, this study aimed to investigate the influence of different aquatic environmental conditions (pH, mineral content) and growth phases on the cultivation-free differentiation between water-conditioned Pseudomonas spp. by applying Raman microspectroscopy. The final dataset was comprised of over 7500 single-cell Raman spectra, including the species Pseudomonas aeruginosa, P. fluorescens and P. putida, in order to prove the feasibility of the introduced approach. The collection of spectra was standardized by automated measurements of viable stained bacterial cells. The discrimination was influenced by the growth phase at the beginning of the water adaptation period and by the type of mineral water. Different combinations of the parameters were tested and they resulted in accuracies of up to 85% for the identification of P. aeruginosa from independent samples by applying chemometric analysis.
authors: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, D-07743 Jena, Germany; InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, D-07743 Jena, Germany.
authors: Anja
authors: A
authors: Silge
authors: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, D-07743 Jena, Germany; InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, D-07743 Jena, Germany.
authors: Wilm
authors: W
authors: Schumacher
authors: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, D-07743 Jena, Germany; InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, D-07743 Jena, Germany.
authors: Petra
authors: P
authors: Rösch
authors: Analytical Sciences Department, Nestlé Research Center, Vers-chez-les-blanc, 1000 Lausanne 26, Switzerland.
authors: Paulo A
authors: PA
authors: Da Costa Filho
authors: Food Safety & Quality Department, Nestlé Research Center, Vers-chez-les-blanc, 1000 Lausanne 26, Switzerland.
authors: Cédric
authors: C
authors: Gérard
authors: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, D-07743 Jena, Germany; Leibniz Institute of Photonic Technology, Albert-Einstein-Strasse 9, D-07702 Jena, Germany; InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, D-07743 Jena, Germany. Electronic address: juergen.popp@uni-jena.de.
authors: Jürgen
authors: J
authors: Popp
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000145:classification*; D011551:Pseudomonas fluorescens / Q000737:chemistry / Q000145:classification; D016958:Pseudomonas putida / Q000737:chemistry / Q000145:classification; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014871:Water Microbiology
keywords: Chemometrics; Cultivation-free identification; Natural mineral water; Pseudomonas aeruginosa; Raman microspectroscopy