title: Cloning and molecular characterization of scorpion Buthus martensi venom hyaluronidases: a novel full-length and diversiform noncoding isoforms.
abstract: Hyaluronidase is a common component of scorpion venom and has been considered as "spreading factor" that promotes a fast penetration of the venom in the anaphylactic reaction. In the current study, a novel full-length of hyaluronidase BmHYI and three noncoding isoforms of BmHYII, BmHYIII and BmHYIV were cloned by using a combined strategy based on peptide sequencing and Rapid Amplification of cDNA Ends (RACE). BmHYI has 410 amino acid residues containing the catalytic, positional and five potential N-glycosylation sites. The deduced protein sequence of BmHYI shares significant identity with venom hyaluronidases from bees and snakes. The phylogenetic analysis showed early divergence and independent evolution of BmHYI from other hyaluronidases. An extraordinarily high level of sequence similarity was detected among four sequences. But, BmHYII, BmHYIII and BmHYIV were short of stop-codon in the open reading frame and poly(A) signal in the 3' end.
authors: Basal Medical College of Nanyang Medical University, Nanyang, 473041 Henan Province, China. Electronic address: xiaxichao8336@163.com.
authors: Xichao
authors: X
authors: Xia
authors: Basal Medical College of Nanyang Medical University, Nanyang, 473041 Henan Province, China.
authors: Rongzhi
authors: R
authors: Liu
authors: Basal Medical College of Nanyang Medical University, Nanyang, 473041 Henan Province, China.
authors: Yi
authors: Y
authors: Li
authors: Basal Medical College of Nanyang Medical University, Nanyang, 473041 Henan Province, China.
authors: Shipeng
authors: S
authors: Xue
authors: Basal Medical College of Nanyang Medical University, Nanyang, 473041 Henan Province, China.
authors: Qingchun
authors: Q
authors: Liu
authors: Basal Medical College of Nanyang Medical University, Nanyang, 473041 Henan Province, China.
authors: Xiao
authors: X
authors: Jiang
authors: Basal Medical College of Nanyang Medical University, Nanyang, 473041 Henan Province, China.
authors: Wenjuan
authors: W
authors: Zhang
authors: Basal Medical College of Nanyang Medical University, Nanyang, 473041 Henan Province, China.
authors: Ke
authors: K
authors: Ding
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020134:Catalytic Domain; D003001:Cloning, Molecular; D006821:Hyaluronoglucosaminidase / Q000737:chemistry / Q000235:genetics*; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D012604:Scorpion Venoms / Q000737:chemistry / Q000235:genetics*; D012605:Scorpions / Q000201:enzymology* / Q000235:genetics
chemical_list: D007527:Isoenzymes; D012604:Scorpion Venoms; D006821:Hyaluronoglucosaminidase
keywords: Buthus martensi; Cloning and molecular characterization; Hyaluronidases; Noncoding isoforms