title: Evaluation of commercial polyclonal- and monoclonal-antibody-based immunohistochemical tests for 2 genotypes of Porcine circovirus type 2 and comparison with in-situ hybridization assays.
abstract: The objective of the present study was to evaluate polyclonal- and monoclonal-antibody-based immunohistochemical (IHC) tests for the detection of 2 genotypes of Porcine circovirus type 2 (PCV2), a and b, in formalin-fixed, paraffin-embedded lymph-node tissue from pigs with experimental or natural postweaning multisystemic wasting syndrome and to compare the IHC results with those of in-situ hybridization (ISH) assays. The ISH assays proved more sensitive than the IHC tests for the detection of PCV2a and PCV2b. According to these findings, polyclonal-antibody-based IHC testing is the most practical routine diagnostic method for the detection of PCV2 regardless of genotype because IHC testing is less technically complex than ISH testing. However, ISH assays are useful to differentiate between PCV2a and PCV2b in surveillance programs for the monitoring of PCV2 in swine herds.
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, 151-742, Seoul, Republic of Korea (Seo, Han, Oh, Kang, Park, Chae); College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-Gu, 130-701, Seoul, Republic of Korea (Joo, Kim); Department of Food and Nutrition, Chung-Ang University, Nae-Ri 72-1, Daedeok-Myeon, 456-756, Gyeonggi-Do, Republic of Korea (Lee).
authors: Hwi Won
authors: HW
authors: Seo
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, 151-742, Seoul, Republic of Korea (Seo, Han, Oh, Kang, Park, Chae); College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-Gu, 130-701, Seoul, Republic of Korea (Joo, Kim); Department of Food and Nutrition, Chung-Ang University, Nae-Ri 72-1, Daedeok-Myeon, 456-756, Gyeonggi-Do, Republic of Korea (Lee).
authors: Kiwon
authors: K
authors: Han
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, 151-742, Seoul, Republic of Korea (Seo, Han, Oh, Kang, Park, Chae); College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-Gu, 130-701, Seoul, Republic of Korea (Joo, Kim); Department of Food and Nutrition, Chung-Ang University, Nae-Ri 72-1, Daedeok-Myeon, 456-756, Gyeonggi-Do, Republic of Korea (Lee).
authors: Yeonsu
authors: Y
authors: Oh
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, 151-742, Seoul, Republic of Korea (Seo, Han, Oh, Kang, Park, Chae); College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-Gu, 130-701, Seoul, Republic of Korea (Joo, Kim); Department of Food and Nutrition, Chung-Ang University, Nae-Ri 72-1, Daedeok-Myeon, 456-756, Gyeonggi-Do, Republic of Korea (Lee).
authors: Ikjae
authors: I
authors: Kang
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, 151-742, Seoul, Republic of Korea (Seo, Han, Oh, Kang, Park, Chae); College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-Gu, 130-701, Seoul, Republic of Korea (Joo, Kim); Department of Food and Nutrition, Chung-Ang University, Nae-Ri 72-1, Daedeok-Myeon, 456-756, Gyeonggi-Do, Republic of Korea (Lee).
authors: Changhoon
authors: C
authors: Park
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, 151-742, Seoul, Republic of Korea (Seo, Han, Oh, Kang, Park, Chae); College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-Gu, 130-701, Seoul, Republic of Korea (Joo, Kim); Department of Food and Nutrition, Chung-Ang University, Nae-Ri 72-1, Daedeok-Myeon, 456-756, Gyeonggi-Do, Republic of Korea (Lee).
authors: Hye Eun
authors: HE
authors: Joo
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, 151-742, Seoul, Republic of Korea (Seo, Han, Oh, Kang, Park, Chae); College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-Gu, 130-701, Seoul, Republic of Korea (Joo, Kim); Department of Food and Nutrition, Chung-Ang University, Nae-Ri 72-1, Daedeok-Myeon, 456-756, Gyeonggi-Do, Republic of Korea (Lee).
authors: Sung-Hoon
authors: SH
authors: Kim
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, 151-742, Seoul, Republic of Korea (Seo, Han, Oh, Kang, Park, Chae); College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-Gu, 130-701, Seoul, Republic of Korea (Joo, Kim); Department of Food and Nutrition, Chung-Ang University, Nae-Ri 72-1, Daedeok-Myeon, 456-756, Gyeonggi-Do, Republic of Korea (Lee).
authors: Bog-Hieu
authors: BH
authors: Lee
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, 151-742, Seoul, Republic of Korea (Seo, Han, Oh, Kang, Park, Chae); College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-Gu, 130-701, Seoul, Republic of Korea (Joo, Kim); Department of Food and Nutrition, Chung-Ang University, Nae-Ri 72-1, Daedeok-Myeon, 456-756, Gyeonggi-Do, Republic of Korea (Lee).
authors: Chanhee
authors: C
authors: Chae
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D017925:Circovirus / Q000145:classification / Q000235:genetics*; D005557:Formaldehyde; D005838:Genotype; D007150:Immunohistochemistry / Q000662:veterinary*; D017403:In Situ Hybridization / Q000662:veterinary*; D016612:Paraffin Embedding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D016707:Tissue Fixation
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D005557:Formaldehyde