title: Effect of Achyranthes bidentata Blume on 3T3-L1 Adipogenesis and Rats Fed with a High-Fat Diet.
abstract: The present study investigated the antiobesity effect of Achyranthes bidentata Blume root water extract in a 3T3-L1 adipocyte differentiation model and rats fed with a high-fat diet. To investigate the effect of Achyranthes bidentata Blume on adipogenesis in vitro, differentiating 3T3-L1 cells in adipocyte-induction media were treated every two days with Achyranthes bidentata Blume at various concentrations (1 to 25  μ g/mL) for eight days. We found that Achyranthes bidentata Blume root inhibited 3T3-L1 adipocyte differentiation without affecting cell viability, and Western blot analysis revealed that phospho-Akt expression was markedly decreased, whereas there was no significant change in perilipin expression. Furthermore, administration of Achyranthes bidentata Blume root (0.5 g/kg body weight for six weeks) to rats fed with a high-fat diet significantly reduced body weight gain without affecting food intake, and the level of triglyceride was significantly decreased when compared to those in rats fed with only a high-fat diet. These results suggest that Achyranthes bidentata Blume root water extract could have a beneficial effect on inhibition of adipogenesis and controlling body weight in rats fed with a high-fat diet.
authors: Department of East-West Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea.
authors: Sang Deog
authors: SD
authors: Oh
authors: Department of Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin, Republic of Korea.
authors: Mihyun
authors: M
authors: Kim
authors: Department of East-West Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea ; Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors: Byung-Il
authors: BI
authors: Min
authors: Department of East-West Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea.
authors: Gi Soon
authors: GS
authors: Choi
authors: Acupuncture & Meridian Science Research Center, Kyung Hee University, Seoul, Republic of Korea ; Division of Homeostatic Development, National Institute for Physiological Sciences, Okazaki, Japan.
authors: Sun-Kwang
authors: SK
authors: Kim
authors: Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors: Hyunsu
authors: H
authors: Bae
authors: Department of Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin, Republic of Korea.
authors: Chulhun
authors: C
authors: Kang
authors: Department of Oriental Pediatrics, Kyung Hee Medical Center, Kyung Hee University, Seoul, Republic of Korea.
authors: Deok-Gon
authors: DG
authors: Kim
authors: Department of Family Medicine, CHA University School of Medicine, Seoul, Republic of Korea.
authors: Byoung-Jin
authors: 0000-0003-1733-5301
authors: BJ
authors: Park
authors: Department of East-West Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea ; Department of Integrative Medicine, CHA University School of Medicine, 442 Dosan-daero, Gangnam-gu, Seoul 135-948, Republic of Korea.
authors: Chang Keun
authors: CK
authors: Kim