title: Rapid and sensitive determination of levofloxacin in microsamples of human plasma by high-performance liquid chromatography and its application in a pharmacokinetic study.
abstract: A rapid, sensitive and simple high-performance liquid chromatographic assay with ultraviolet detection was developed for the quantification of levofloxacin in microsamples (100 μL) of human plasma. The extraction procedure included a protein precipitation technique and a short chromatographic running time (4.5 min). Analyses were carried out on a Symmetry C18 column using a mixture of acetonitrile and 0.01 m potassium dihydrogen aqueous solution (pH 3.4; 14:86 v/v) as mobile phase. The method provided specificity and was linear (r ≥ 0.9992) over the concentration range 0.1-12 µg/mL. The average absolute recovery was 93.59%. The intra- and inter-day coefficients of variation were <6%. Additionally, levofloxacin was stable in all evaluations. The usefulness of this method was demonstrated in a pharmacokinetic study of levofloxacin in healthy adult volunteers. The present method offers two main advantages: (a) the use of microsamples reduces the total volume of blood to be collected from patients; and (b) it provides a good cost-effectiveness ratio. It is concluded that the method is rapid, simple, sensitive, economical and suitable for the determination of levofloxacin in human plasma using a small volume of sample.
authors: Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, México City, México.
authors: José Carlos
authors: JC
authors: Aguilar-Carrasco
authors: Jessica
authors: J
authors: Hernández-Pineda
authors: Juan Miguel
authors: JM
authors: Jiménez-Andrade
authors: Francisco Javier
authors: FJ
authors: Flores-Murrieta
authors: Miriam Del Carmen
authors: Mdel C
authors: Carrasco-Portugal
authors: Jorge Skiold
authors: JS
authors: López-Canales
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D064704:Levofloxacin / Q000097:blood* / Q000493:pharmacokinetics*; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D064704:Levofloxacin
keywords: HPLC; human plasma; levofloxacin; microsamples; pharmacokinetics