title: Position-specific modification with imidazolyl group on10-23 DNAzyme realized catalytic activity enhancement.
abstract: Nucleoside analogues with imidazolyl and histidinyl groups were synthesized for site-specific modification on the catalytic core of 10-23 DNAzyme. The distinct position-dependent effect of imidazolyl group was observed. Positive effect at A9 position was always observed. The pH- and Mg(2+)-dependence of the imidazolyl-modified DNAzymes suggested that imidazolyl group in 10-23 DNAzyme probably plays a dual role, its hydrogen bonding ability and spacial occupation play the favorable influence on the catalytic conformation of the modified DNAzymes. This research demonstrated that the catalytic performance of DNAzymes could be enhanced by incorporation of additional functional groups. Chemical modification is a feasible approach toward more efficient DNAzymes for therapeutic and biotechnological applications.
authors: College of Life Sciences, Guizhou University, Guiyang 550025, China.
authors: Zhiwen
authors: Z
authors: Li
authors: School of Pharmacological Sciences, Guangxi Medical University, Nanning 530021, China.
authors: Yang
authors: Y
authors: Liu
authors: College of Life Sciences, Guizhou University, Guiyang 550025, China.
authors: Gaofeng
authors: G
authors: Liu
authors: College of Life Sciences, Guizhou University, Guiyang 550025, China.
authors: Junfei
authors: J
authors: Zhu
authors: School of Pharmacological Sciences, Guangxi Medical University, Nanning 530021, China; Beijing Institute of Pharmacology and Toxicology, Taiping Road 27, Beijing 100850, China.
authors: Zhibing
authors: Z
authors: Zheng
authors: College of Life Sciences, Guizhou University, Guiyang 550025, China.
authors: Ying
authors: Y
authors: Zhou
authors: College of Life Sciences, Guizhou University, Guiyang 550025, China; Beijing Institute of Pharmacology and Toxicology, Taiping Road 27, Beijing 100850, China. Electronic address: hejunlin@bmi.ac.cn.
authors: Junlin
authors: J
authors: He
mesh_terms: D002384:Catalysis; D002942:Circular Dichroism; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry*; D007700:Kinetics; D008274:Magnesium / Q000737:chemistry; D044366:Transition Temperature
chemical_list: D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D007093:Imidazoles; C402582:RNA-cleaving DNA 10-23; C029899:imidazole; D008274:Magnesium
keywords: 10–23 DNAzyme; Chemical modification; Functional nucleic acids; Imidazolyl group; Observed rate constant