title: Prompt bone marrow-derived mesenchymal stem cell therapy enables early porcine heart function recovery from acute myocardial infarction.
abstract: Impact of early bone marrow-derived mesenchymal stem cell (BMDMSC) implantation on left ventricular (LV) function after AMI was studied.Twelve mini-pigs were equally divided into placebo (AMI through left coronary artery ligation) and cell-treated groups [BMDMSCs (3.0 × 10(7)) implanted into infarct area (IA)] with myocardium harvested by post-AMI day 90. Six healthy animals served as controls.On post-AMI day 90, magnetic resonance imaging showed a lower LV ejection fraction but higher LV dimensions in the placebo group (P < 0.003) that also had increased IAs but reduced wall thickness (P < 0.005). Pro-apoptotic gene expressions (Bax, caspase-3) and apoptotic nucleus number in IAs and peri-IAs were highest in the placebo group (P < 0.001). Inflammatory biomarker expressions (MMP-9, oxidized protein, CD40+ cells) were highest, whereas those of angiogenesis (VEGF, CD31+ cells, SDF-1α, CXCR4) and myocardium-preservation (connexin43, troponin-I, cytochrome-C) were lowest in the placebo group (P < 0.01).BMDMSC implantation preserved LV function and alleviated remodeling at post-AMI day 90.
authors: Division of Cardiology, Department of Internal Medicine, Xiamen Chang Gung Memorial Hospital.
authors: Chang-Qing
authors: CQ
authors: Fan
authors: Steve
authors: S
authors: Leu
authors: Jiunn-Jye
authors: JJ
authors: Sheu
authors: Yen-Yi
authors: YY
authors: Zhen
authors: Tzu-Hsien
authors: TH
authors: Tsai
authors: Yung-Lung
authors: YL
authors: Chen
authors: Sheng-Ying
authors: SY
authors: Chung
authors: Han-Tan
authors: HT
authors: Chai
authors: Cheuk-Kwan
authors: CK
authors: Sun
authors: Jenq-Lin
authors: JL
authors: Yang
authors: Hsueh-Wen
authors: HW
authors: Chang
authors: Sheung-Fat
authors: SF
authors: Ko
authors: Hon-Kan
authors: HK
authors: Yip
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D004195:Disease Models, Animal; D006352:Heart Ventricles; D015552:Injections, Intralesional; D019028:Magnetic Resonance Imaging, Cine; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009203:Myocardial Infarction / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D020127:Recovery of Function; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors; D016896:Treatment Outcome; D019210:Troponin I / Q000378:metabolism; D016277:Ventricular Function, Left / Q000502:physiology*; D020257:Ventricular Remodeling / Q000502:physiology
chemical_list: D015415:Biomarkers; D019210:Troponin I