title: Platelet-rich plasma favors proliferation of canine adipose-derived mesenchymal stem cells in methacrylate-endcapped caprolactone porous scaffold niches.
abstract: Osteoarticular pathologies very often require an implementation therapy to favor regeneration processes of bone, cartilage and/or tendons. Clinical approaches performed on osteoarticular complications in dogs constitute an ideal model for human clinical translational applications. The adipose-derived mesenchymal stem cells (ASCs) have already been used to accelerate and facilitate the regenerative process. ASCs can be maintained in vitro and they can be differentiated to osteocytes or chondrocytes offering a good tool for cell replacement therapies in human and veterinary medicine. Although ACSs can be easily obtained from adipose tissue, the amplification process is usually performed by a time consuming process of successive passages. In this work, we use canine ASCs obtained by using a Bioreactor device under GMP cell culture conditions that produces a minimum of 30 million cells within 2 weeks. This method provides a rapid and aseptic method for production of sufficient stem cells with potential further use in clinical applications. We show that plasma rich in growth factors (PRGF) treatment positively contributes to viability and proliferation of canine ASCs into caprolactone 2-(methacryloyloxy) ethyl ester (CLMA) scaffolds. This biomaterial does not need additional modifications for cASCs attachment and proliferation. Here we propose a framework based on a combination of approaches that may contribute to increase the therapeutical capability of stem cells by the use of PRGF and compatible biomaterials for bone and connective tissue regeneration.
authors: Neuronal Regeneration Lab, Centro de Investigación Principe Felipe, València 46012, Spain. frodriguez@cipf.es.
authors: Francisco Javier
authors: FJ
authors: Rodríguez-Jiménez
authors: Neuronal Regeneration Lab, Centro de Investigación Principe Felipe, València 46012, Spain. teresavalsan@gmail.com.
authors: Teresa
authors: T
authors: Valdes-Sánchez
authors: Medicine and Surgery Department, CEU-Cardenal Herrera University, Moncada 46115, Spain. jcarrill@uch.ceu.es.
authors: José M
authors: JM
authors: Carrillo
authors: Medicine and Surgery Department, CEU-Cardenal Herrera University, Moncada 46115, Spain. mrubio@uch.ceu.es.
authors: Mónica
authors: M
authors: Rubio
authors: Centre for Biomaterials and Tissue Enginering, Universitat Politècnica de València, València E-46022, Spain. mmonleon@ter.upv.es.
authors: Manuel
authors: M
authors: Monleon-Prades
authors: Centre for Biomaterials and Tissue Enginering, Universitat Politècnica de València, València E-46022, Spain. dugarcru@ter.upv.es.
authors: Dunia Mercedes
authors: DM
authors: García-Cruz
authors: Fundación García Cugat, Barcelona 08006, Spain. montse.garcia@sportrauma.com.
authors: Montserrat
authors: M
authors: García
authors: Fundación García Cugat, Barcelona 08006, Spain. secretaria@sportrauma.com.
authors: Ramón
authors: R
authors: Cugat
authors: Neuronal Regeneration Lab, Centro de Investigación Principe Felipe, València 46012, Spain. vmorenom@cipf.es.
authors: Victoria
authors: V
authors: Moreno-Manzano