title: Serine protease inhibitor (SERPIN) B1 suppresses cell migration and invasion in glioma cells.
abstract: The serine protease inhibitor (SERPIN) B1 is expressed in numerous human tumors, but little is known regarding its role in the pathophysiology of glioma. In this paper, we report that SERPINB1 expression was down-regulated in high-grade human glioma tissue samples and glioblastoma cell lines. To investigate the role of SERPINB1 in glioma migration and invasion, we generated human glioma cell lines in which SERPINB1 was either overexpressed or depleted. Overexpression of SERPINB1 suppressed, while elimination of SERPINB1 promoted, the migration and invasion of glioma cells. SERPINB1 inhibited glioma migration and invasion probably by dampening the expression of matrix metalloproteinase-2 (MMP-2). Molecular data showed that the effect of SERPINB1 in glioma cells might be mediated via sustained inactivation of the phosphorylation of focal adhesion kinase (FAK) involved in the downregulation of the expressions of MMP-2. In a multivariate analysis, high SERPINB1 expression was showed to be associated with good prognosis in glioma. In conclusion, our data suggest that SERPINB1 negatively regulates glioma cell migration and invasion probably by abrogating the expression of MMP-2 and the activation of FAK. We suggest that SERPINB1 may offer the application in clinical medicine.
authors: Department of Neurosurgery, Fudan University Huashan Hospital, National Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Shanghai Medical College, Fudan University, Shanghai 200000, China.
authors: Gao
authors: G
authors: Huasong
authors: Department of Pathology, Subei People's Hospital, Yangzhou 225001, Jiangsu, China.
authors: Ding
authors: D
authors: Zongmei
authors: Department of Pathology, Subei People's Hospital, Yangzhou 225001, Jiangsu, China.
authors: Huang
authors: H
authors: Jianfeng
authors: Department of Pathology, Subei People's Hospital, Yangzhou 225001, Jiangsu, China.
authors: Qiu
authors: Q
authors: Xiaojun
authors: Department of Pathology, Subei People's Hospital, Yangzhou 225001, Jiangsu, China.
authors: Guo
authors: G
authors: Jun
authors: Department of Pathology, Subei People's Hospital, Yangzhou 225001, Jiangsu, China.
authors: Guan
authors: G
authors: Sun
authors: Department of Pathology, Medical College, Nantong University, Nantong 226001, Jiangsu, China. Electronic address: meimei19871001@sina.com.
authors: Wang
authors: W
authors: Donglin
authors: Department of Neurosurgery, Fudan University Huashan Hospital, National Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Shanghai Medical College, Fudan University, Shanghai 200000, China. Electronic address: fudan_jhzhu@126.com.
authors: Zhu
authors: Z
authors: Jianhong
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D005260:Female; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D005909:Glioblastoma / Q000378:metabolism; D005910:Glioma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D015843:Serpins / Q000378:metabolism*
chemical_list: D015843:Serpins; C075177:SERPINB1 protein, human; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D020778:Matrix Metalloproteinase 2
keywords: Glioma; Invasion; MMP-2; Migration; SERPINB1