title: Collagenase-3 expression in periapical lesions: an immunohistochemical study.
abstract: Collagenase-3 (matrix metalloproteinase-13) is a metalloproteinase (MMP) that is associated with bone lesions and exhibits variable expression patterns in odontogenic cysts; it may play a role in regulating focal proliferation and maturation of jaw cyst epithelium. We studied the localization, staining intensity and distribution of collagenase-3 in 13 periapical granulomas with epithelium, 16 periapical granulomas without epithelium and 10 radicular cysts using archived formalin fixed, paraffin embedded tissues. A monoclonal antibody against human collagenase-3 was used to evaluate its expression. Immunohistochemical staining intensities of collagenase-3 in all periapical lesions were (-), 4 (10%); (+), 1 (3%); (++), 22 (56%) and (+++), 12 (31%); differences were not statistically significant. Immunohistochemical distribution of collagenase-3 in epithelial cells was (-), 17 (44%); (+), 17 (44%); (++), 5 (13%); in fibroblasts it was (-), 8 (20%); (+), 23 (59%); (++), 8 (21%); in plasma cells it was (-), 7 (18%); (+), 22 (56%); (++), 10 (26%); in macrophages it was (-), 7 (18%); (+), and 15 (38%); and (++), 17 (44%). Statistically significant differences were found in epithelial cells (p = 0.00) and fibroblasts (p = 0.02), whereas differences were not statistically significant for plasma cells and macrophages. Collagenase-3 may play a role in the conversion of a periapical granuloma with epithelium to radicular cyst. MMP's influence not only epithelial rest cell migration, but also invasion of various stromal cells into granulomatous tissue.
authors: Himachal Institute of Dental Sciences , Paontasahib, HP , India.
authors: G
authors: G
authors: Bhalla
authors: M S
authors: MS
authors: Astekar
authors: G
authors: G
authors: Ramesh
authors: P
authors: P
authors: Kaur
authors: G V
authors: GV
authors: Sowmya
mesh_terms: D002465:Cell Movement / Q000502:physiology; D004848:Epithelium / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008264:Macrophages / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism*; D010484:Periapical Granuloma / Q000201:enzymology* / Q000473:pathology; D011842:Radicular Cyst / Q000201:enzymology* / Q000473:pathology
chemical_list: C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13
keywords: MMP-13; collagenase 3; periapical granuloma; periapical lesions; radicular cyst