title: [Experimental research on establishing a blood glucose fluctuation model in diabetic rats].
abstract: OBJECTIVE
  To establish a blood glucose fluctuation model in diabetic rats.
  
  
  METHODS
  Male SD rats were randomly divided into 2 groups, namely normal group and diabetes group. Rat model of diabetes was established by single intraperitoneal injection of streptozotocin (STZ) and then divided into sustained high blood glucose group and blood glucose fluctuation group. Rat model of blood glucose fluctuation was established by subcutaneous injection with regular insulin or gavaging of glucose twice daily in diabetic rats. The general condition, body weight, daily blood glucose levels of 5 different times, daily average blood glucose (MBG), standard deviation of daily average blood glucose (SDBG), the maximum amplitude of glycemic excursions (LAGE), fasting serum insulin (FINS) and pancreatic tissue pathology were observed.
  
  
  RESULTS
  Rats in blood glucose fluctuation group and sustained high blood glucose group developed symptoms of polyphagia, polyuria and polydipsia. Though significant differences in body weight were observed at different time (P<0.01), no significant differences were found among the three groups (P>0.05). After 6 weeks of blood glucose fluctuation, MBG, SDBG and LAGE in blood glucose fluctuation group and sustained high blood glucose group were increased significantly than those in normal group (P<0.01), the level of FINS decreased markedly (P<0.05). SDBG and LAGE in blood glucose fluctuation group were higher than those in sustained high blood glucose group (P<0.01). Islet of diabetic rat became atrophy, irregular shape, sparse distribution, and decreased in number, and the changes were more obvious in blood glucose fluctuation group.
  
  
  CONCLUSIONS
  Rat model of blood glucose fluctuation can be successfully established by subcutaneous injection of regular insulin of gavage of glucose twice daily in diabetic rats.
authors: Anhui University of Chinese Medicine, Hefei 230038, China.E-mail: anhuiwuyuanjie@126.com.
authors: Yuanjie
authors: Y
authors: Wu
authors: Yuanbo
authors: Y
authors: Wu
authors: Shuguo
authors: S
authors: Zheng
authors: Yufeng
authors: Y
authors: Wang
authors: Xiaoli
authors: X
authors: Liu
authors: Xiaojun
authors: X
authors: Zhang
authors: Mingan
authors: M
authors: Lv
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental; D005215:Fasting; D005947:Glucose / Q000008:administration & dosage; D007328:Insulin / Q000008:administration & dosage; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D007328:Insulin; D013311:Streptozocin; D005947:Glucose