title: Hemin offers neuroprotection through inducing exogenous neuroglobin in focal cerebral hypoxic-ischemia in rats.
abstract: OBJECTIVE
  To investigate the inducible effect of hemin on exogenous neuroglobin (Ngb) in focal cerebral hypoxic-ischemia in rats.
  
  
  METHODS
  125 healthy SD rats were randomly divided into five groups: sham-operation control group, operation group, hemin treatment group, exogenous Ngb treatment group, and hemin and exogenous Ngb joint treatment group. Twenty-four hours after focal cerebral hypoxic-ischemia, Ngb expression was evaluated by immunocytochemistry, RT-PCR, and western blot analyses, while the brain water content and infarct volume were examined.
  
  
  RESULTS
  Immunocytochemistry, RT-PCR, and western blot analyses showed more pronounced Ngb expression in the hemin and exogenous Ngb joint operation group than in the hemin or exogenous Ngb individual treatment groups, thus producing significant differences in brain water content and infarct volume (p<0.05).
  
  
  CONCLUSIONS
  Hemin may be beneficial in protecting against focal cerebral hypoxic-ischemia through inducing the expression of exogenous Ngb.
authors: Department of Neurosurgery, First Affiliated Hospital of Chongqing Medical University Chongqing, 400016, China.
authors: Xue
authors: X
authors: Song
authors: Department of Neurosurgery, First Affiliated Hospital of Chongqing Medical University Chongqing, 400016, China.
authors: Rui
authors: R
authors: Xu
authors: Department of Neurosurgery, First Affiliated Hospital of Chongqing Medical University Chongqing, 400016, China.
authors: Fei
authors: F
authors: Xie
authors: Department of Neurosurgery, First Affiliated Hospital of Chongqing Medical University Chongqing, 400016, China.
authors: Haiyuan
authors: H
authors: Zhu
authors: Department of Neurosurgery, First Affiliated Hospital of Chongqing Medical University Chongqing, 400016, China.
authors: Ji
authors: J
authors: Zhu
authors: Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School Boston, Massachusetts 02115, USA.
authors: Xin
authors: X
authors: Wang
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D001929:Brain Edema / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D019610:Cytoprotection; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics; D006427:Hemin / Q000494:pharmacology*; D020925:Hypoxia-Ischemia, Brain / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D020244:Infarction, Middle Cerebral Artery / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D000078787:Neuroglobin; D018696:Neuroprotective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D018696:Neuroprotective Agents; D012333:RNA, Messenger; D006427:Hemin; D005914:Globins
keywords: Hemin; focal cerebral hypoxic-ischemia; neuroglobin; neuroprotection