title: Broadly neutralizing antibody VRC01 prevents HIV-1 transmission from plasmacytoid dendritic cells to CD4 T lymphocytes.
abstract: Plasmacytoid dendritic cells (pDC) poorly replicate human immunodeficiency virus type 1 (HIV-1) but efficiently transfer HIV-1 to adjacent CD4 T lymphocytes. We found that coculture with T lymphocytes downregulates SAMHD1 expression, enhances HIV-1 replication, and increases pDC maturation and alpha interferon (IFN-α) secretion. HIV-1 transfer to T lymphocytes is inhibited by broadly neutralizing antibody VRC01 with efficiency similar to that of cell-free infection of T lymphocytes. Interestingly, prevention of HIV-1 transmission by VRC01 retains IFN-α secretion. These results emphasize the multiple functions of VRC01 in protection against HIV-1 acquisition.
authors: INSERM U1109, Fédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Université de Strasbourg, Strasbourg, France binsu.paris7@gmail.com c.moog@unistra.fr.
authors: Bin
authors: B
authors: Su
authors: INSERM U1110, Fédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Université de Strasbourg, Strasbourg, France.
authors: Alexandre
authors: A
authors: Lederle
authors: INSERM U1109, Fédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Université de Strasbourg, Strasbourg, France.
authors: Géraldine
authors: G
authors: Laumond
authors: INSERM U1109, Fédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Université de Strasbourg, Strasbourg, France.
authors: Camille
authors: C
authors: Ducloy
authors: INSERM U1109, Fédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Université de Strasbourg, Strasbourg, France.
authors: Sylvie
authors: S
authors: Schmidt
authors: INSERM U1109, Fédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Université de Strasbourg, Strasbourg, France.
authors: Thomas
authors: T
authors: Decoville
authors: INSERM U1109, Fédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Université de Strasbourg, Strasbourg, France binsu.paris7@gmail.com c.moog@unistra.fr.
authors: Christiane
authors: C
authors: Moog
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D057134:Antibodies, Neutralizing / Q000494:pharmacology*; D000914:Antibodies, Viral / Q000494:pharmacology*; D000080908:Broadly Neutralizing Antibodies; D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000821:virology*; D015483:HIV Antibodies / Q000494:pharmacology*; D015658:HIV Infections / Q000517:prevention & control / Q000821:virology*; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology*; D006801:Humans
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000080908:Broadly Neutralizing Antibodies; D015483:HIV Antibodies; C578595:VRC01 monoclonal antibody