title: Rhodovulum mangrovi sp. nov., a phototrophic alphaproteobacterium isolated from a mangrove forest sediment sample.
abstract: A novel Gram-staining-negative, purple non-sulfur bacterium, strain AK41(T), was isolated from a sediment sample collected from Coringa mangrove forest, Andhra Pradesh, India. A red-brownish-coloured culture was obtained on modified Pfennig medium after enrichment with 2 % NaCl and 0.3 % pyruvate under 2000 lx illumination. Individual cells were ovoid-rod-shaped and non-motile. Bacteriochlorophyll a and carotenoids of the spheroidene series were present as photosynthetic pigments. Strain AK41(T) was halophilic and grew photoheterotrophically with a number of organic compounds as carbon sources and electron donors. It was unable to grow photoautotrophically. It did not utilize sulfide or thiosulfate as electron donors. The fatty acids were found to be dominated by C16 : 0 and C18 : 1ω7c. Strain AK41(T) contained phosphatidylglycerol, phosphatidylethanolamine, an unknown aminolipid and four unknown lipids as polar lipids. Q-10 was the predominant respiratory quinone. The DNA G+C content of strain AK41(T) was 68.9 mol%. 16S rRNA gene sequence analysis indicated that strain AK41(T) was a member of the genus Rhodovulum and was closely related to Rhodovulum sulfidophilum, with 96.0 % similarity to the type strain; the 16S rRNA gene sequence similarity to the type strains of other species of the genus Rhodovulum was 93.9-95.8 %. Phylogenetic analyses indicated that strain AK41(T) clustered with the type strains of Rhodovulum marinum, Rdv. kholense, Rdv. sulfidophilum and Rdv. visakhapatnamense with sequence similarity of 95.9-96.2 %. Based on data from the current study, strain AK41(T) is proposed to represent a novel species of the genus Rhodovulum, for which the name Rhodovulum mangrovi sp. nov. is proposed. The type strain of Rhodovulum mangrovi is AK41(T) ( = MTCC 11825(T) = JCM 19220(T)).
authors: Microbial Type Culture Collection and Gene Bank, CSIR - Institute of Microbial Technology, Sector 39A, Chandigarh - 160 036, India.
authors: P
authors: P
authors: Nupur
authors: CSIR - National Institute of Oceanography, Regional Centre, 176 Lawsons Bay Colony, Visakhapatnam - 530 017, India.
authors: T N R
authors: TNR
authors: Srinivas
authors: Nippon Medical School, Department of Biology, Kosugi-cho, Nakahara, Kawasaki 211-0063, Japan.
authors: S
authors: S
authors: Takaichi
authors: Microbial Type Culture Collection and Gene Bank, CSIR - Institute of Microbial Technology, Sector 39A, Chandigarh - 160 036, India.
authors: P
authors: P
authors: Anil Kumar
mesh_terms: D032407:Avicennia / Q000382:microbiology; D015373:Bacterial Typing Techniques; D025541:Bacteriochlorophyll A / Q000737:chemistry; D001482:Base Composition; D002338:Carotenoids / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041903:Rhodovulum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D053833:Wetlands
chemical_list: D025541:Bacteriochlorophyll A; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C005637:spheroidene; D002338:Carotenoids; C025204:Ubiquinone Q2