title: Effects of C18 Fatty Acids on Intracellular Ca(2+) Mobilization and Histamine Release in RBL-2H3 Cells.
abstract: To investigate the underlying mechanisms of C18 fatty acids (stearic acid, oleic acid, linoleic acid and α-linolenic acid) on mast cells, we measured the effect of C18 fatty acids on intracellular Ca(2+) mobilization and histamine release in RBL-2H3 mast cells. Stearic acid rapidly increased initial peak of intracellular Ca(2+) mobilization, whereas linoleic acid and α-linolenic acid gradually increased this mobilization. In the absence of extracellular Ca(2+), stearic acid (100 µM) did not cause any increase of intracellular Ca(2+) mobilization. Both linoleic acid and α-linolenic acid increased intracellular Ca(2+) mobilization, but the increase was smaller than that in the presence of extracellular Ca(2+). These results suggest that C18 fatty acid-induced intracellular Ca(2+) mobilization is mainly dependent on extracellular Ca(2+) influx. Verapamil dose-dependently inhibited stearic acid-induced intracellular Ca(2+) mobilization, but did not affect both linoleic acid and α-linolenic acid-induced intracellular Ca(2+) mobilization. These data suggest that the underlying mechanism of stearic acid, linoleic acid and α-linolenic acid on intracellular Ca(2+) mobilization may differ. Linoleic acid and α-linolenic acid significantly increased histamine release. Linoleic acid (C18:2: ω-6)-induced intracellular Ca(2+) mobilization and histamine release were more prominent than α-linolenic acid (C18:3: ω-3). These data support the view that the intake of more α-linolenic acid than linoleic acid is useful in preventing inflammation.
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors: Myung Chul
authors: MC
authors: Kim
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors: Min Gyu
authors: MG
authors: Kim
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors: Young Soo
authors: YS
authors: Jo
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors: Ho Sun
authors: HS
authors: Song
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors: Tae In
authors: TI
authors: Eom
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors: Sang Soo
authors: SS
authors: Sim
keywords: C18 fatty acids; Ca2+ mobilization; Histamine release; PLC assay