title: Toxic effects of Microgramma vacciniifolia rhizome lectin on Artemia salina, human cells, and the schistosomiasis vector Biomphalaria glabrata.
abstract: The present study evaluated the toxicity of Microgramma vacciniifolia rhizome lectin (MvRL) to Artemia salina, human tumour cell lines (larynx epidermoid carcinoma Hep-2, NCI-H292 lung mucoepidermoid carcinoma, and chronic myelocytic leukaemia K562), and normal peripheral blood mononuclear cells (PBMCs), as well as to Biomphalaria glabrata embryos and adults. MvRL was toxic to A. salina (LC50=159.9 μg/mL), and exerted cytotoxic effects on NCI-H292 cells (IC50=25.23 μg/mL). The lectin (1-100 μg/mL) did not affect the viability of K562 and Hep-2 tumour cells, as well as of PBMCs. MvRL concentration of 1, 10, and 100 μg/mL promoted malformations (mainly exogastrulation) in 7.8%, 22.5%, and 27.7% of embryos, respectively, as well as delayed embryo development in 42.0%, 69.5%, and 54.7% of embryos, respectively. MvRL at a concentration of 100 μg/mL killed B. glabrata embryos (17.7%) and adults (25%). Further, MvRL damaged B. glabrata reproductive processes, which was evidenced by observations that snails exposed to the lectin (100 μg/mL) deposited fewer eggs than those in the control group, and approximately 40% of the deposited eggs exhibited malformations. Comparison of these results with that from A. salina assay indicates that MvRL is adulticidal at the concentration range which is toxic to environment. In conclusion, the cytotoxicity of MvRL on tumour cell and absence of toxicity to normal cell indicate its potential as chemotherapeutic drug. Also, the study revealed that the lectin is able to promote deleterious effects on B. glabrata embryos at environmentally safe concentrations.
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Recife, PE 50670-420, Brazil.
authors: Lidiane Pereira
authors: LP
authors: de Albuquerque
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Recife, PE 50670-420, Brazil.
authors: Emmanuel Viana
authors: EV
authors: Pontual
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Recife, PE 50670-420, Brazil.
authors: Giselly Maria de Sá
authors: GM
authors: Santana
authors: Departamento de Biofísica e Radiobiologia-CCB, Universidade Federal de Pernambuco, Recife, PE, Brazil.
authors: Luanna Ribeiro Santos
authors: LR
authors: Silva
authors: Departamento de Antibióticos-CCB, Universidade Federal de Pernambuco, Recife, PE, Brazil.
authors: Jaciana dos Santos
authors: Jdos S
authors: Aguiar
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Recife, PE 50670-420, Brazil.
authors: Luana Cassandra Breitenbach Barroso
authors: LC
authors: Coelho
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Recife, PE 50670-420, Brazil; Laboratório de Imunomodulação e Novas Abordagens Terapêuticas, Núcleo de Pesquisa em Inovação Terapêutica (NUPIT), Recife, PE, Brazil.
authors: Moacyr Jesus Barreto de Melo
authors: MJ
authors: Rêgo
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Recife, PE 50670-420, Brazil; Laboratório de Imunomodulação e Novas Abordagens Terapêuticas, Núcleo de Pesquisa em Inovação Terapêutica (NUPIT), Recife, PE, Brazil.
authors: Maira Galdino da Rocha
authors: MG
authors: Pitta
authors: Departamento de Antibióticos-CCB, Universidade Federal de Pernambuco, Recife, PE, Brazil.
authors: Teresinha Gonçalves
authors: TG
authors: da Silva
authors: Departamento de Biofísica e Radiobiologia-CCB, Universidade Federal de Pernambuco, Recife, PE, Brazil.
authors: Ana Maria Mendonça de Albuquerque
authors: AM
authors: Melo
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Recife, PE 50670-420, Brazil.
authors: Thiago Henrique
authors: TH
authors: Napoleão
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Recife, PE 50670-420, Brazil. Electronic address: ppaivaufpe@yahoo.com.br.
authors: Patrícia Maria Guedes
authors: PM
authors: Paiva
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000977:Antiparasitic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001156:Artemia / Q000187:drug effects*; D001700:Biomphalaria / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004199:Disease Vectors; D006801:Humans; D037102:Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D029621:Polypodiaceae / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*; D016019:Survival Analysis
chemical_list: D000970:Antineoplastic Agents; D000977:Antiparasitic Agents; D037102:Lectins
keywords: Antitumour activity; Rhizome lectin