title: Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins.
abstract: In this study, a recombinant modified vaccinia virus Ankara vector expressing a chimeric multi-antigen was obtained and evaluated as a candidate vaccine in homologous and heterologous prime-boost immunizations with a recombinant protein cocktail. The chimeric multi-antigen comprises immunodominant B and T cell regions of three Babesia bovis proteins. Humoral and cellular immune responses were evaluated in mice to compare the immunogenicity induced by different immunization schemes. The best vaccination scheme was achieved with a prime of protein cocktail and a boost with the recombinant virus. This scheme induced high level of specific IgG antibodies and secreted IFN and a high degree of activation of IFNγ(+) CD4(+) and CD8(+) specific T cells. This is the first report in which a novel vaccine candidate was constructed based on a rationally designed multi-antigen and evaluated in a prime-boost regime, optimizing the immune response necessary for protection against bovine babesiosis.
authors: Instituto de Biotecnología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, De Los Reseros y Dr. Nicolás Repetto s/no., 1686, Hurlingham, Buenos Aires, Argentina. Electronic address: jose@inta.gob.ar.
authors: José Manuel
authors: JM
authors: Jaramillo Ortiz
authors: Instituto de Biotecnología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, De Los Reseros y Dr. Nicolás Repetto s/no., 1686, Hurlingham, Buenos Aires, Argentina.
authors: María Paula
authors: MP
authors: Del Médico Zajac
authors: Instituto de Biotecnología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, De Los Reseros y Dr. Nicolás Repetto s/no., 1686, Hurlingham, Buenos Aires, Argentina.
authors: Flavia Adriana
authors: FA
authors: Zanetti
authors: Instituto de Biotecnología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, De Los Reseros y Dr. Nicolás Repetto s/no., 1686, Hurlingham, Buenos Aires, Argentina.
authors: María Paula
authors: MP
authors: Molinari
authors: Instituto de Biotecnología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, De Los Reseros y Dr. Nicolás Repetto s/no., 1686, Hurlingham, Buenos Aires, Argentina.
authors: María José
authors: MJ
authors: Gravisaco
authors: Instituto de Biotecnología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, De Los Reseros y Dr. Nicolás Repetto s/no., 1686, Hurlingham, Buenos Aires, Argentina.
authors: Gabriela
authors: G
authors: Calamante
authors: Instituto de Biotecnología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, De Los Reseros y Dr. Nicolás Repetto s/no., 1686, Hurlingham, Buenos Aires, Argentina.
authors: Silvina Elizabeth
authors: SE
authors: Wilkowsky
mesh_terms: D000818:Animals; D016793:Babesia bovis / Q000235:genetics / Q000276:immunology*; D001404:Babesiosis / Q000276:immunology / Q000517:prevention & control*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D002642:Chick Embryo; D006224:Cricetinae; D005347:Fibroblasts / Q000276:immunology; D005822:Genetic Vectors / Q000276:immunology; D007111:Immunity, Cellular; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016052:Protozoan Vaccines / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D013552:Swine; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D007074:Immunoglobulin G; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D007371:Interferon-gamma
keywords: Babesia bovis; Heterologous prime-boost; Modified vaccinia virus Ankara (MVA); Multiantigen; Recombinant proteins