title: hesperidin induces paraptosis like cell death in hepatoblastoma, HepG2 Cells: involvement of ERK1/2 MAPK [corrected].
abstract: Hesperidin, a natural flavonoid abundantly present in Citrus is known for its anti-cancer, anti-oxidant and anti-inflammatory properties. In this study we examined the effect of hesperidin on HepG2 cells. HepG2 cells treated with various concentration of hesperidin undergo a distinct type of programed cell death. Cytoplasmic vacuolization, mitochondria and endoplasmic reticulum swelling and uncondensed chromatin were observed in hesperidin treated cells. DNA electrophoresis show lack of DNA fragmentation and western blot analysis demonstrates lack of caspase activation and PARP cleavage. It was observed that hesperidin induced cell death is nonautophagic and also activate mitogen activated protein kinase ERK1/2. Taken together, the data indicate that hesperidin induces paraptosis like cell death in HepG2 cells with the activation of ERK1/2. Thus our finding suggests that hesperidin inducing paraptosis may offer an alternative tool in human liver carcinoma therapy.
authors: Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Gyeongsang National University, Gazwa, Jinju, Republic of Korea.
authors: Silvia
authors: S
authors: Yumnam
authors: Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Gyeongsang National University, Gazwa, Jinju, Republic of Korea.
authors: Hyeon Soo
authors: HS
authors: Park
authors: Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Gyeongsang National University, Gazwa, Jinju, Republic of Korea.
authors: Mun Ki
authors: MK
authors: Kim
authors: Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Gyeongsang National University, Gazwa, Jinju, Republic of Korea.
authors: Arulkumar
authors: A
authors: Nagappan
authors: Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Gyeongsang National University, Gazwa, Jinju, Republic of Korea.
authors: Gyeong Eun
authors: GE
authors: Hong
authors: Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Gyeongsang National University, Gazwa, Jinju, Republic of Korea.
authors: Ho Jeong
authors: HJ
authors: Lee
authors: Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongnam Regional Cancer Center, Gyeongsang National University Hospital, Jinju, Republic of Korea.
authors: Won Sup
authors: WS
authors: Lee
authors: Department of Nursing Science, International University of Korea, Jinju, Republic of Korea.
authors: Eun Hee
authors: EH
authors: Kim
authors: Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Gyeongsang National University, Gazwa, Jinju, Republic of Korea.
authors: Jae Hyeon
authors: JH
authors: Cho
authors: Department of Chemistry, Research Institute of Life Science, Gyeongsang National University, Jinju, Republic of Korea.
authors: Sung Chul
authors: SC
authors: Shin
authors: Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Gyeongsang National University, Gazwa, Jinju, Republic of Korea.
authors: Gon Sup
authors: GS
authors: Kim
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D016923:Cell Death; D004721:Endoplasmic Reticulum / Q000187:drug effects; D056945:Hep G2 Cells; D006569:Hesperidin / Q000494:pharmacology*; D006801:Humans; D008928:Mitochondria / Q000187:drug effects; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D006569:Hesperidin; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3