title: Evaluation of fungicides enestroburin and SYP1620 on their inhibitory activities to fungi and oomycetes and systemic translocation in plants.
abstract: Enestroburin and SYP1620 are newly developed strobilurin chemicals carrying fungicidal activity and need to be fully characterized in activities of anti-oomycete or anti-fungi, disease prevention and systemic translocation in planta. Their inhibitory activities were examined by amending the chemical in agar media, on which selected plant pathogens were grown and mycelial growth were measured. Effective concentrations for 50% inhibition (EC50) of mycelial growth were calculated to determine the level of fungicide sensitivity of the pathogen. Azoxystrobin was used as control. To examine the prevention and systemic translocation in plants, the fungicides were either sprayed on wheat leaves or dipped on wheat roots, which then were detected using high performance liquid chromatography. All the three fungicides inhibited mycelial growth of Sphacelotheca reiliana, Phytophthora infestans, Peronophythora litchi, and Magnaporthe oryzae, with EC50 values ranging from 0.02 to 2.84μg/ml; EC50 of SYP1620 was significantly lower than that of azoxystrobin and enestroburin on Valsa mali, Gaeumannomyces graminis, Alternaria solani, and Colletotrichun orbiculare. The three QoI fungicides showed strong inhibitory activities on spore germination against the 13 pathogens tested and were highly effective on biotrophic pathogens tested. Enestroburin and SYP1620 penetrated and spread in wheat leaves, but the penetration and translocation levels were lower compared to azoxystrobin. The three fungicides were all rapidly taken up by wheat roots and transported upwards, with greater fungicide concentrations in roots than in stems and leaves. The results indicate that enestroburin and SYP1620 are systemic fungicides that inhibit a broad spectrum of fungi and oomycetes.
authors: Department of Plant Pathology, China Agricultural University, Beijing, China.
authors: Pengfei
authors: P
authors: Liu
authors: Department of Plant Pathology, China Agricultural University, Beijing, China.
authors: Haiqiang
authors: H
authors: Wang
authors: Department of Plant Pathology, China Agricultural University, Beijing, China.
authors: Yuxin
authors: Y
authors: Zhou
authors: Department of Plant Pathology, China Agricultural University, Beijing, China.
authors: Qingxiao
authors: Q
authors: Meng
authors: State Key Laboratory of the Discovery and Development of Novel Pesticide, China Shenyang Research Institute of the Chemical Industry, Shenyang, China.
authors: Naiguo
authors: N
authors: Si
authors: School of Food and Agriculture, University of Maine, Orono, ME, USA.
authors: Jianjun J
authors: JJ
authors: Hao
authors: Department of Plant Pathology, China Agricultural University, Beijing, China. Electronic address: seedling@cau.edu.cn.
authors: Xili
authors: X
authors: Liu
mesh_terms: D001692:Biological Transport; D002851:Chromatography, High Pressure Liquid; D018553:Cucumis sativus / Q000378:metabolism / Q000382:microbiology; D005658:Fungi / Q000145:classification / Q000187:drug effects* / Q000502:physiology; D005659:Fungicides, Industrial / Q000378:metabolism / Q000494:pharmacology*; D008689:Methacrylates / Q000378:metabolism / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D025282:Mycelium / Q000187:drug effects* / Q000502:physiology; D009868:Oomycetes / Q000187:drug effects* / Q000502:physiology; D010091:Oximes / Q000494:pharmacology*; D010666:Phenylpropionates / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D018517:Plant Roots / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism / Q000494:pharmacology; D013045:Species Specificity; D013172:Spores, Fungal / Q000187:drug effects / Q000502:physiology; D000073739:Strobilurins; D014908:Triticum / Q000378:metabolism / Q000382:microbiology
chemical_list: D005659:Fungicides, Industrial; D008689:Methacrylates; D010091:Oximes; D010666:Phenylpropionates; D011743:Pyrimidines; D000073739:Strobilurins; C000595440:enestroburin; C087670:azoxystrobin
keywords: Inhibitory activity; Preventive activity; Quinone outer inhibitors; Systemic translocation