title: Molecular cloning and expression analysis of ferritin, heavy polypeptide 1 gene from duck (Anas platyrhynchos).
abstract: H-ferritin is a core subunit of the iron storage protein ferritin, and is related to the pathogenesis of malignant diseases. A differential expressed sequence tag of the ferritin, heavy polypeptide 1 gene (FTH1) was obtained from our previously constructed suppression subtractive cDNA library from 3-day-old ducklings challenged with duck hepatitis virus type I (DHV-1). The expression and function of FTH1 in immune defense against infection remains largely unknown in ducks. In this study, the full-length duFTH1 cDNA was obtained using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. It consisted of 153 basepairs (bp) 5'untranslated region (UTR), 183 bp 3'UTR, and 546 bp open reading frame that encodes a single protein of 181 amino acid residues. duFTH1 shares high similarity with FTH1 genes from other vertebrates. The amino acid sequence possesses the conserved domain of typical ferritin H subunits, including seven metal ligands in the ferroxidase center, one iron binding region signature, and a potential bio-mineralization residue (Thy(29)). Moreover, in agreement with a previously reported ferritin H subunit, we identified an iron response element in the 5'UTR. RT-PCR analyses revealed duFTH1 mRNA is widely expressed in various tissues. Real-time quantitative polymerase chain reaction analyses suggested that duFTH1 mRNA is significantly up-regulated in the liver after DHV-1 injection or polyriboinosinic polyribocytidylic acid (polyI:C) treatment, reaching a peak 4 h post-infection, and dropping progressively and returning to normal after 24 h. Our findings suggest that duFTH1 functions as an iron chelating protein subunit in duck and contributes to the innate immune responses against viral infections.
authors: Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Qi
authors: Q
authors: Xu
authors: Yang
authors: Y
authors: Chen
authors: Yang
authors: Y
authors: Zhang
authors: Yi Yu
authors: YY
authors: Tong
authors: Zheng Yang
authors: ZY
authors: Huang
authors: Wen Ming
authors: WM
authors: Zhao
authors: Xiu Jun
authors: XJ
authors: Duan
authors: Xiu
authors: X
authors: Li
authors: Guo Bin
authors: GB
authors: Chang
authors: Guo Hong
authors: GH
authors: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001052:Apoferritins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004372:Ducks / Q000235:genetics* / Q000821:virology; D015723:Gene Library; D004371:Hepatitis Virus, Duck / Q000302:isolation & purification; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy / Q000276:immunology; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000188:drug therapy / Q000276:immunology; D011070:Poly I-C / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D017422:Sequence Analysis, DNA; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D007502:Iron Chelating Agents; D012333:RNA, Messenger; D001052:Apoferritins; D007501:Iron; D011070:Poly I-C