title: An electrochemical ELISA-like immunosensor for miRNAs detection based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes.
abstract: We design an electrochemical immunosensor for miRNA detection, based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes. An original immunological approach is followed, using antibodies directed to DNA.RNA hybrids. An electrochemical ELISA-like amplification strategy was set up using a secondary antibody conjugated to horseradish peroxidase (HRP). Hydroquinone is oxidized into benzoquinone by the HRP/H2O2 catalytic system. In turn, benzoquinone is electroreduced into hydroquinone at the electrode. The catalytic reduction current is related to HRP amount immobilized on the surface, which itself is related to miRNA.DNA surface density on the electrode. This architecture, compared to classical optical detection, lowers the detection limit down to 10 fM. Two miRNAs were studied: miR-141 (a prostate biomarker) and miR-29b-1 (a lung cancer biomarker).
authors: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; USTH, University of Science and Technology of Hanoi, 18 Hoang Quoc Viet, Hanoi, Vietnam; School of Chemical Engineering, Hanoi University of Science and Technology, 1st Dai Co Viet Road, Hanoi, Vietnam.
authors: H V
authors: HV
authors: Tran
authors: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
authors: B
authors: B
authors: Piro
authors: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
authors: S
authors: S
authors: Reisberg
authors: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
authors: L
authors: L
authors: Huy Nguyen
authors: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
authors: T
authors: T
authors: Dung Nguyen
authors: Université Paris 11, INSERM U-1014, Hôpital Paul Brousse 94800 Villejuif, France.
authors: H T
authors: HT
authors: Duc
authors: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: mcpham@univ-paris-diderot.fr.
authors: M C
authors: MC
authors: Pham
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry
chemical_list: D035683:MicroRNAs; D037742:Nanotubes, Carbon; D010087:Oxides; D006046:Gold; D006108:Graphite
keywords: Carbon nanotubes; Electrochemical biosensor; Immunodetection; Label-free detection; Reduced graphene oxide; miRNA