title: Effect of diallyl disulfide on acute gastric mucosal damage induced by alcohol in rats.
abstract: This study investigated the gastroprotective effects of diallyl disulfide (DADS), a secondary organosulfur compound derived from garlic (Allium sativum L.) on experimental model of ethanol (EtOH)-induced gastric ulcer in rats. The antiulcerogenic activity of DADS was evaluated by gross/histopathological inspection, pro-inflammatory cytokines, and lipid peroxidation with antioxidant enzyme activities in the stomach. DADS (100 mg/kg) was administered by oral gavage 2 h prior to EtOH treatment (5 ml/kg). The animals were killed 1 h after receiving EtOH treatment. Pretreatment with DADS attenuated EtOH-induced gastric mucosal injury, as evidenced by decreased severity of hemorrhagic lesions and gastric ulcer index upon visual inspection. DADS also prevented histopathological alterations and gastric apoptotic changes caused by EtOH. An increase in tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase was observed in the gastric tissues of EtOH-treated rats that coincided with increased serum TNF-α and interleukin 6 levels. In contrast, DADS effectively suppressed production of pro-inflammatory mediators induced by EtOH. Furthermore, DADS prevented the formation of gastric malondialdehyde and the depletion of reduced glutathione content and restored antioxidant enzyme activities, such as catalase, glutathione peroxidase, and glutathione reductase in the gastric tissues of EtOH-treated rats. These results indicate that DADS prevents gastric mucosal damage induced by acute EtOH administration in rats and that the protective effects of DADS may be due to its potent antioxidant and anti-inflammatory activities.
authors: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors: I-C
authors: IC
authors: Lee
authors: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors: H-S
authors: HS
authors: Baek
authors: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors: S-H
authors: SH
authors: Kim
authors: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors: C
authors: C
authors: Moon
authors: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors: S-H
authors: SH
authors: Park
authors: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors: S-H
authors: SH
authors: Kim
authors: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Republic of Korea.
authors: I-S
authors: IS
authors: Shin
authors: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: S-C
authors: SC
authors: Park
authors: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea toxkim@jnu.ac.kr.
authors: J-C
authors: JC
authors: Kim
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D004220:Disulfides / Q000494:pharmacology / Q000627:therapeutic use*; D000431:Ethanol; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000097:blood / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
chemical_list: D000498:Allyl Compounds; D000897:Anti-Ulcer Agents; D004220:Disulfides; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol; C028009:diallyl disulfide; D002374:Catalase; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D005980:Glutathione Reductase; D005978:Glutathione
keywords: Ethanol; diallyl disulfide; gastric mucosal damages; oxidative stress