title: Cellular behavior as a dynamic field for exploring bone bioengineering: a closer look at cell-biomaterial interface.
abstract: Bone is a highly dynamic and specialized tissue, capable of regenerating itself spontaneously when afflicted by minor injuries. Nevertheless, when major lesions occur, it becomes necessary to use biomaterials, which are not only able to endure the cellular proliferation and migration, but also to substitute the original tissue or integrate itself to it. With the life expectancy growth, regenerative medicine has been gaining constant attention in the reconstructive field of dentistry and orthopedy. Focusing on broadening the therapeutic possibilities for the regeneration of injured organs, the development of biomaterials allied with the applicability of gene therapy and bone bioengineering has been receiving vast attention over the recent years. The progress of cellular and molecular biology techniques gave way to new-guided therapy possibilities. Supported by multidisciplinary activities, tissue engineering combines the interaction of physicists, chemists, biologists, engineers, biotechnologist, dentists and physicians with common goals: the search for materials that could promote and lead cell activity. A well-oriented combining of scaffolds, promoting factors, cells, together with gene therapy advances may open new avenues to bone healing in the near future. In this review, our target was to write a report bringing overall concepts on tissue bioengineering, with a special attention to decisive biological parameters for the development of biomaterials, as well as to discuss known intracellular signal transduction as a new manner to be explored within this field, aiming to predict in vitro the quality of the host cell/material and thus contributing with the development of regenerative medicine.
authors: Laboratório de Bioensaios e Dinâmica Celular, Depto. Química e Bioquímica, Instituto de Biociência, Universidade Estadual Paulista, UNESP, Campus Botucatu, Botucatu, SP, Brazil; Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy.
authors: Sara
authors: S
authors: Gemini-Piperni
authors: Excellion Biomedical Services, Petrópolis, RJ, Brazil.
authors: Esther Rieko
authors: ER
authors: Takamori
authors: Department of Oral Surgery, Fluminense Federal University, Niteroi, Brazil.
authors: Suelen Cristina
authors: SC
authors: Sartoretto
authors: Extracellular Matrix Biology and Cellular Interaction Group, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
authors: Katiúcia B S
authors: KB
authors: Paiva
authors: Instituto Nacional de Metrologia, Normalização e Qualidade Industrial (INMETRO), Diretoria de Programas (DIPRO)/Bioengenharia, Xerém, RJ, Brazil.
authors: José Mauro
authors: JM
authors: Granjeiro
authors: Department of Biological Sciences, Bauru Dental School, University of São Paulo (USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, SP 17012-901, Brazil.
authors: Rodrigo Cardoso
authors: RC
authors: de Oliveira
authors: Laboratório de Bioensaios e Dinâmica Celular, Depto. Química e Bioquímica, Instituto de Biociência, Universidade Estadual Paulista, UNESP, Campus Botucatu, Botucatu, SP, Brazil. Electronic address: wzambuzzi@ibb.unesp.br.
authors: Willian Fernando
authors: WF
authors: Zambuzzi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration / Q000502:physiology*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D011474:Prosthesis Design; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials
keywords: Bioengineering; Biomaterials; Bone; Cell behavior; Dentistry; Osteoblast; Regenerative medicine; Scaffolds; Signal transduction