title: Oleic Acid, deglycosylated vitamin D-binding protein, nitric oxide: a molecular triad made lethal to cancer.
abstract: BACKGROUND
  Oleic Acid (OA) has been shown to have anticancer properties mediated by interaction with proteins such as α-lactalbumin and lactoferrins. Therefore, we synthesized complexes of OA and Gc protein-derived macrophage activating factor (GcMAF) that inhibits per se cancer cell proliferation and metastatic potential. We hypothesised that OA-GcMAF complexes could exploit the anticancer properties of both OA and GcMAF in a synergistic manner. We postulated that the stimulating effects of GcMAF on macrophages might lead to release of nitric oxide (NO).
  
  
  METHODS
  Patients with advanced cancer were treated at the Immuno Biotech Treatment Centre with OA-GcMAF-based integrative immunotherapy in combination with a low-carbohydrate, high-protein diet, fermented milk products containing naturally-produced GcMAF, Vitamin D3, omega-3 fatty acids and low-dose acetylsalicylic acid.
  
  
  RESULTS
  Measuring the tumour by ultrasonographic techniques, we observed a decrease of tumour volume of about 25%.
  
  
  CONCLUSIONS
  These observations demonstrate that OA, GcMAF and NO can be properly combined and specifically delivered to advanced cancer patients with significant effects on immune system stimulation and tumour volume reduction avoiding harmful side-effects.
authors: Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, Italy Immuno Biotech Ltd, Guernsey, Channel Islands, UK.
authors: Marco
authors: M
authors: Ruggiero
authors: Macro Innovations Ltd, Cambridge, UK.
authors: Emma
authors: E
authors: Ward
authors: Macro Innovations Ltd, Cambridge, UK.
authors: Rodney
authors: R
authors: Smith
authors: Department of Experimental and Clinical Medicine, University of Firenze, Firenze, Italy jacopo.branca@libero.it.
authors: Jacopo J V
authors: JJ
authors: Branca
authors: Immuno Biotech Ltd, Guernsey, Channel Islands, UK.
authors: David
authors: D
authors: Noakes
authors: Department of Experimental and Clinical Medicine, University of Firenze, Firenze, Italy.
authors: Gabriele
authors: G
authors: Morucci
authors: Naturheilzentrum, Bayreuth, Germany.
authors: Margit
authors: M
authors: Taubmann
authors: Macro Innovations Ltd, Cambridge, UK.
authors: Lynda
authors: L
authors: Thyer
authors: Department of Experimental and Clinical Medicine, University of Firenze, Firenze, Italy.
authors: Stefania
authors: S
authors: Pacini
mesh_terms: D001241:Aspirin / Q000008:administration & dosage; D002762:Cholecalciferol / Q000008:administration & dosage; D003131:Combined Modality Therapy; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004044:Dietary Proteins / Q000008:administration & dosage; D004357:Drug Synergism; D005260:Female; D006801:Humans; D007167:Immunotherapy; D016215:Macrophage-Activating Factors / Q000008:administration & dosage* / Q000737:chemistry; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000178:diet therapy / Q000188:drug therapy / Q000378:metabolism / Q000628:therapy*; D009569:Nitric Oxide / Q000378:metabolism*; D019301:Oleic Acid / Q000008:administration & dosage* / Q000737:chemistry; D014809:Vitamin D-Binding Protein / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D016215:Macrophage-Activating Factors; D014809:Vitamin D-Binding Protein; C101908:vitamin D-binding protein-macrophage activating factor; D002762:Cholecalciferol; D019301:Oleic Acid; D009569:Nitric Oxide; D001241:Aspirin
keywords: Gc protein-derived macrophage activating factor; Oleic acid; human cancer; macrophages; nitric oxide; vitamin D