title: Regulation of plant stem cell quiescence by a brassinosteroid signaling module.
abstract: The quiescent center (QC) maintains the activity of the surrounding stem cells within the root stem cell niche, yet specific molecular players sustaining the low rate of QC cell division remain poorly understood. Here, we identified a R2R3-MYB transcription factor, BRAVO (BRASSINOSTEROIDS AT VASCULAR AND ORGANIZING CENTER), acting as a cell-specific repressor of QC divisions in the primary root of Arabidopsis. Ectopic BRAVO expression restricts overall root growth and ceases root regeneration upon damage of the stem cells, demonstrating the role of BRAVO in counteracting Brassinosteroid (BR)-mediated cell division in the QC cells. Interestingly, BR-regulated transcription factor BES1 (BRI1-EMS SUPRESSOR 1) directly represses and physically interacts with BRAVO in vivo, creating a switch that modulates QC divisions at the root stem cell niche. Together, our results define a mechanism for BR-mediated regulation of stem cell quiescence in plants.
authors: Department of Molecular Genetics, Center for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain.
authors: Josep
authors: J
authors: Vilarrasa-Blasi
authors: Department of Molecular Genetics, Center for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain.
authors: Mary-Paz
authors: MP
authors: González-García
authors: Departament d'Estructura i Constituents de la Matèria, Facultat de Física, Universitat de Barcelona, 08028 Barcelona, Spain.
authors: David
authors: D
authors: Frigola
authors: Department of Molecular Genetics, Center for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain.
authors: Norma
authors: N
authors: Fàbregas
authors: Department of Molecular Genetics, Center for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain.
authors: Konstantinos G
authors: KG
authors: Alexiou
authors: Biomedical Genomic Group, Research Unit on Biomedical Informatics (GRIB), Barcelona Biomedical Research park (PRBB), Dr. Aiguader 88, 08003 Barcelona, Spain.
authors: Nuria
authors: N
authors: López-Bigas
authors: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, 31326 Castanet-Tolosan, France; CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, 31326 Castanet-Tolosan, France.
authors: Susana
authors: S
authors: Rivas
authors: Fédération de Recherche 3450, Plateforme Imagerie, Pôle de Biotechnologie Végétale, 31320 Castanet-Tolosan, France.
authors: Alain
authors: A
authors: Jauneau
authors: Center for Organismal Studies (COS), University of Heidelberg, 69120 Heidelberg, Germany.
authors: Jan U
authors: JU
authors: Lohmann
authors: Department of Biology, HHMI and Duke Center for Systems Biology, Duke University, Durham, NC 27708, USA.
authors: Philip N
authors: PN
authors: Benfey
authors: Departament d'Estructura i Constituents de la Matèria, Facultat de Física, Universitat de Barcelona, 08028 Barcelona, Spain.
authors: Marta
authors: M
authors: Ibañes
authors: Department of Molecular Genetics, Center for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain. Electronic address: ana.cano@cragenomica.es.
authors: Ana I
authors: AI
authors: Caño-Delgado
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D060406:Brassinosteroids / Q000494:pharmacology*; D002455:Cell Division; D049109:Cell Proliferation; D047369:Chromatin Immunoprecipitation; D004797:Enzyme-Linked Immunosorbent Assay; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D047468:Immunoprecipitation; D008962:Models, Theoretical; D009154:Mutation / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D055153:Stem Cell Niche / Q000187:drug effects*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D015415:Biomarkers; D060406:Brassinosteroids; D010937:Plant Growth Regulators; D012333:RNA, Messenger