title: Development of an integrated reporting system for verifying hemolysis, icterus, and lipemia in clinical chemistry results.
abstract: BACKGROUND
  Hemolysis, icterus, and lipemia (HIL) cause preanalytical interference and vary unpredictably with different analytical equipments and measurement methods. We developed an integrated reporting system for verifying HIL status in order to identify the extent of interference by HIL on clinical chemistry results.
  
  
  METHODS
  HIL interference data from 30 chemical analytes were provided by the manufacturers and were used to generate a table of clinically relevant interference values that indicated the extent of bias at specific index values (alert index values). The HIL results generated by the Vista 1500 system (Siemens Healthcare Diagnostics, USA), Advia 2400 system (Siemens Healthcare Diagnostics), and Modular DPE system (Roche Diagnostics, Switzerland) were analyzed and displayed on physicians' personal computers.
  
  
  RESULTS
  Analytes 11 and 29 among the 30 chemical analytes were affected by interference due to hemolysis, when measured using the Vista and Modular systems, respectively. The hemolysis alert indices for the Vista and Modular systems were 0.1-25.8% and 0.1-64.7%, respectively. The alert indices for icterus and lipemia were <1.4% and 0.7% in the Vista system and 0.7% and 1.0% in the Modular system, respectively.
  
  
  CONCLUSIONS
  The HIL alert index values for chemical analytes varied depending on the chemistry analyzer. This integrated HIL reporting system provides an effective screening tool for verifying specimen quality with regard to HIL and simplifies the laboratory workflow.
authors: Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, Korea.
authors: Dong Hoon
authors: DH
authors: Shin
authors: Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Juwon
authors: J
authors: Kim
authors: Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea. ; Department of Medical Information Development, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Young
authors: Y
authors: Uh
authors: Department of Medical Information Development, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Se Il
authors: SI
authors: Lee
authors: Department of Medical Information Development, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Dong Min
authors: DM
authors: Seo
authors: Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Kab Seung
authors: KS
authors: Kim
authors: Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Jae Yun
authors: JY
authors: Jang
authors: Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Man Hee
authors: MH
authors: Lee
authors: Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Kwang Ro
authors: KR
authors: Yoon
authors: Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Kap Jun
authors: KJ
authors: Yoon
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D006949:Hyperlipidemias / Q000378:metabolism / Q000473:pathology*; D007565:Jaundice / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D006454:Hemoglobins
keywords: Hemolysis; Icterus; Lipemia; Preanalytical interference