title: Ascorbic acid protects against anxiogenic-like effect induced by methylmercury in zebrafish: action on the serotonergic system.
abstract: To evaluate the protector effect of ascorbic acid (AA) against anxiogenic-like effect induced by methylmercury (MeHg) exposure, adult zebrafish were treated with AA (2 mg g(-1), intraperitoneal [i.p.]) before MeHg administration (1.0 μg g(-1), i.p.). Groups were tested for the light/dark preference as a behavioral model of anxiety, and the content of serotonin and its oxidized metabolite tryptamine-4,5-dione (T-4,5-D) in the brain was determined by high-performance liquid chromatography. MeHg has produced a marked anxiogenic profile in both tests, and this effect was accompanied by a decrease in the extracellular levels of serotonin, and an increase in the extracellular levels of T-4,5-D. Added to this, a marked increase in the formation of a marker of oxidative stress accompanied these parameters. Interestingly, the anxiogenic-like effect and biochemical alterations induced by MeHg were blocked by pretreatment with AA. These results for the first time demonstrated the potential protector action of AA in neurobehavioral and neurochemical alterations induced by methylmecury exposure demonstrating that zebrafish model could be used as an important tool for testing substances with neuroprotector actions.
authors: 1 Laboratory of Neuroendocrinology, Institute of Biological Sciences, Federal University of Para , Belém, Pará, Brazil .
authors: Bruna
authors: B
authors: Puty
authors: Caio
authors: C
authors: Maximino
authors: Alódia
authors: A
authors: Brasil
authors: Waldo Lucas Luz
authors: WL
authors: da Silva
authors: Amauri
authors: A
authors: Gouveia
authors: Karen Renata Matos
authors: KR
authors: Oliveira
authors: Evander de Jesus Oliveira
authors: Ede J
authors: Batista
authors: Maria Elena
authors: ME
authors: Crespo-Lopez
authors: Fernando Allan F
authors: FA
authors: Rocha
authors: Anderson Manoel
authors: AM
authors: Herculano
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001007:Anxiety / Q000139:chemically induced*; D001205:Ascorbic Acid / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004785:Environmental Pollutants / Q000633:toxicity*; D045563:Indolequinones / Q000378:metabolism; D008767:Methylmercury Compounds / Q000633:toxicity*; D012701:Serotonin / Q000378:metabolism; D014363:Tryptamines / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D004785:Environmental Pollutants; D045563:Indolequinones; D008767:Methylmercury Compounds; D014363:Tryptamines; C060213:tryptamine-4,5-dione; D012701:Serotonin; D001205:Ascorbic Acid; C004925:methylmercuric chloride