title: Expression of p53 and caspase-8 in lens epithelial cells of diabetic cataract.
abstract: OBJECTIVE
  To determine expression of apoptotic factors p53 and caspase-8 in human lens epithelial cells (LECs) of cataract patients with or without diabetic retinopathy (DR), the duration of diabetes mellitus (DM), and the level of glycated hemoglobin (hemoglobin A1c [HbA1c]).
  
  
  METHODS
  St. Mary's Hospital, Catholic University of Korea, Seoul, South Korea.
  
  
  METHODS
  Randomized prospective study.
  
  
  METHODS
  The LECs were isolated during cataract surgery. The isolated samples were classified into 4 groups as follows: patients without DM (Group 1), patients with DM but not DR (Group 2), diabetic patients with nonproliferative DR (Group 3), and diabetic patients with proliferative DR (Group 4). To explore the mechanism of apoptosis, the expressions of p53 and caspase-8 were measured by immunohistochemical staining and compared with the data according to the duration of DM, HbA1c levels, and severity of DR.
  
  
  RESULTS
  All groups comprised 15 eyes. The expressions of P53 and caspase-8 were higher in Groups 2, 3, and 4 than in Group 1 (P<.001). The expressions were statistically significantly increased with a longer duration of DM, higher HbA1c levels, and advanced DR.
  
  
  CONCLUSIONS
  The expressions of P53 and caspase-8 were strong in patients with DM and advanced DR. Knowledge of these relationships may lead to a better understanding of the development of diabetic cataract.
  
  
  BACKGROUND
  No author has a financial or proprietary interest in any material or method mentioned.
authors: From the Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital (Lim, Joo, Kim), and St. Paul's Hospital (Chung), College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 137-701, South Korea.
authors: Sung A
authors: SA
authors: Lim
authors: From the Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital (Lim, Joo, Kim), and St. Paul's Hospital (Chung), College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 137-701, South Korea.
authors: Choun-Ki
authors: CK
authors: Joo
authors: From the Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital (Lim, Joo, Kim), and St. Paul's Hospital (Chung), College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 137-701, South Korea.
authors: Man Soo
authors: MS
authors: Kim
authors: From the Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital (Lim, Joo, Kim), and St. Paul's Hospital (Chung), College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 137-701, South Korea. Electronic address: eyedoc@catholic.ac.kr.
authors: Sung Kun
authors: SK
authors: Chung
mesh_terms: D000368:Aged; D017209:Apoptosis; D001786:Blood Glucose / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism*; D002386:Cataract / Q000378:metabolism* / Q000473:pathology; D003920:Diabetes Mellitus / Q000378:metabolism* / Q000473:pathology; D003930:Diabetic Retinopathy / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D019654:Lens Implantation, Intraocular; D007908:Lens, Crystalline / Q000473:pathology*; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D018918:Phacoemulsification; D011446:Prospective Studies; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D053181:Caspase 8; D006442:Glycated Hemoglobin; D016159:Tumor Suppressor Protein p53; C517652:hemoglobin A1c protein, human; C505530:CASP8 protein, human