title: Molecular epidemiology of NDM-1-producing Enterobacteriaceae and Acinetobacter baumannii isolates from Pakistan.
abstract: The molecular epidemiology of 66 NDM-producing isolates from 2 Pakistani hospitals was investigated, with their genetic relatedness determined using repetitive sequence-based PCR (Rep-PCR). PCR-based replicon typing and screening for antibiotic resistance genes encoding carbapenemases, other β-lactamases, and 16S methylases were also performed. Rep-PCR suggested a clonal spread of Enterobacter cloacae and Escherichia coli. A number of plasmid replicon types were identified, with the incompatibility A/C group (IncA/C) being the most common (78%). 16S methylase-encoding genes were coharbored in 81% of NDM-producing Enterobacteriaceae.
authors: University of Queensland, UQ Centre for Clinical Research, Herston, Queensland, Australia anna.sartor22@gmail.com.
authors: Anna L
authors: AL
authors: Sartor
authors: Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, United Kingdom.
authors: Muhammad W
authors: MW
authors: Raza
authors: Microbiology Department, Armed Forces Institute of Pathology, Rawalpindi, Pakistan.
authors: Shahid A
authors: SA
authors: Abbasi
authors: Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, United Kingdom.
authors: Kathryn M
authors: KM
authors: Day
authors: Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, United Kingdom.
authors: John D
authors: JD
authors: Perry
authors: University of Queensland, UQ Centre for Clinical Research, Herston, Queensland, Australia.
authors: David L
authors: DL
authors: Paterson
authors: University of Queensland, UQ Centre for Clinical Research, Herston, Queensland, Australia.
authors: Hanna E
authors: HE
authors: Sidjabat
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000560:Amdinocillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology; D003428:Cross Infection / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics; D000077726:Doripenem; D004755:Enterobacteriaceae / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D005578:Fosfomycin / Q000494:pharmacology; D006801:Humans; D000077731:Meropenem; D008780:Methyltransferases / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D010154:Pakistan; D013845:Thienamycins / Q000494:pharmacology; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004269:DNA, Bacterial; D013845:Thienamycins; D005578:Fosfomycin; D000077726:Doripenem; D008780:Methyltransferases; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; C063912:carbapenemase; D000077731:Meropenem; D000560:Amdinocillin