title: MiR-126-5p regulates osteolysis formation and stromal cell proliferation in giant cell tumor through inhibition of PTHrP.
abstract: Parathyroid hormone-related protein (PTHrP) has been identified to play a crucial role in osteolysis formation and stromal cell (GCTSC) proliferation in giant cell tumor (GCT). MiR-126-5p is an intronic miRNA identified as tumor suppressor in many tumors, but its role in GCT is poorly understood. We found that miR-126-5p was decreased in GCT and could directly regulate PTHrP expression. Furthermore, miR-126-5p could control osteoclast (OC) differentiation, GCTSC proliferation and osteolysis formation in GCT through negative regulation of PTHrP. Thus, these results suggest that miR-126-5p could directly target PTHrP and have a tumor suppressor function in GCT.
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Wang
authors: W
authors: Zhou
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Huabin
authors: H
authors: Yin
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Ting
authors: T
authors: Wang
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Tielong
authors: T
authors: Liu
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Zhenxi
authors: Z
authors: Li
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Wangjun
authors: W
authors: Yan
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Dianwen
authors: D
authors: Song
authors: Division of Rheumatology, Zhongda Hospital, Dongnan University, Nanjing, China.
authors: Haiyan
authors: H
authors: Chen
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Jia
authors: J
authors: Chen
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Wei
authors: W
authors: Xu
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China. Electronic address: cnspineyang@163.com.
authors: Xinghai
authors: X
authors: Yang
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China. Electronic address: eaglewzp@sina.com.
authors: Zhipeng
authors: Z
authors: Wu
authors: Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China. Electronic address: jianruxiao83@163.com.
authors: Jianru
authors: J
authors: Xiao
mesh_terms: D000818:Animals; D001483:Base Sequence; D001862:Bone Resorption / Q000473:pathology; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D004720:Endonucleases / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005818:Genetic Engineering; D005870:Giant Cell Tumors / Q000150:complications* / Q000235:genetics / Q000473:pathology*; D015726:Giant Cells / Q000378:metabolism / Q000473:pathology; D057809:HEK293 Cells; D006801:Humans; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010014:Osteolysis / Q000209:etiology* / Q000235:genetics / Q000473:pathology*; D044162:Parathyroid Hormone-Related Protein / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D044162:Parathyroid Hormone-Related Protein; D004720:Endonucleases
keywords: Giant cell tumor; Osteoclast differentiation; Osteolysis; PTHrP; miR-126-5p