title: [Assessment of cytotoxicity and biosafety of polyphenolic extracts from olive pits].
abstract: The olive tree is a source of bioactive compounds, both its fruit and its by-products. Some of its compounds have shown health benefits, being objective of this work the evaluation of biosafety in-vitro and in vivo of extracts of olive stones rich in polyphenols.
  
  
  METHODS
  He has been evaluated for cytotoxicity by addition of lyophilized extract dissolved in PBS(0-400 mg/l) to a culture of the cell line THP1-XBlue- CD14 and evaluation of cell viability by the reaction of reduction of resazurin by living cells. Biosecurity has been evaluated in zebrafish, incubating eggs fertilized in 0 to 100 mg/l extract for 24 to 72 hours and measuring parameters: a) lethal (dead embryos, coagulated eggs), b) sublethal (spontaneous movements, pigmentation, edemas) and c) teratogenic (malformations, retardation development).
  
  
  RESULTS
  Cytotoxicity (toxic effect when less than 75% viability) extract bones of olive in the cell line THP1- XBlue-CD14, is in concentrations higher than 50 mg/l, calculating a LD50 (dose lethality 50) more than 800 mg/l. The biosafety of zebrafish embryos exposed to concentrations of extract from 0-100 mg/l showed total viability at 24, 48 and 72 hours post fertilization (hpf), not observed mortality or appreciated embryos with sublethal effects, teratogenic, or advancement or delay in hatching. It can be concluded that the bones of olive extract is highly biosecured until at least 100 mg/l concentrations.
authors: Laboratorio de Biotecnología y Proyectos. Biopartner S.L.. ernesto.cortes@umh.es.
authors: C
authors: C
authors: Veciana Galindo
authors: Departamento Farmacología, Pediatría y Q. Orgánica. Universidad Miguel Hernández.. ernesto.cortes@umh.es.
authors: E
authors: E
authors: Cortés Castell
authors: Departamento de Medicina Clínica. Universidad Miguel Hernández..
authors: L
authors: L
authors: Torro Montell
authors: Departamento de Enfermería y Nutrición. Universidad de Alicante..
authors: E
authors: E
authors: Sirvent Segura
authors: Laboratorio de Biotecnología y Proyectos. Biopartner S.L..
authors: M M
authors: MM
authors: Rizo Baeza
authors: Laboratorio de Biotecnología y Proyectos. Biopartner S.L..
authors: V
authors: V
authors: Gil Guillén
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005612:Freeze Drying; D031658:Olea / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity; D059808:Polyphenols / Q000633:toxicity*; D012639:Seeds / Q000737:chemistry*; D015027:Zebrafish / Q000502:physiology*
chemical_list: D010936:Plant Extracts; D059808:Polyphenols