title: CCN1 enhances angiogenic potency of bone marrow transplantation in a rat model of hindlimb ischemia.
abstract: Implantation of autologous bone marrow mononuclear cells (BM-MNCs) has been performed in ischemic tissues, for stimulation of angiogenesis, but the limited number of BM-MNCs in patients with hindlimb ischemia disease may offset their overall therapeutic efficacy. CCN1 is a novel and essential regulator during angiogenesis. We evaluated whether CCN1 and BM-MNC are capable of promoting angiogenesis in hindlimb ischemia. In this study, we created the rat model of hindlimb ischemia, and then the rats were randomly divided into four groups: CCN1 infusion plus BM-MNC transplantation (CCN1 + BM-MNCs group), CCN1 infusion plus PBS injection (CCN1 group), vehicle infusion plus BM-MNC transplantation (BM-MNCs group) and vehicle infusion plus PBS injection (control group). The combination of CCN1 and BM-MNC therapy could increase blood perfusion, capillary/muscle fiber ratio and tissue oxygenation in ischemic hindlimb. Moreover, CCN1 could not only inhibit the apoptosis of BM-MNCs, but also enhance the adhesiveness of BM-MNCs to HUVEC. Taken together, CCN1 enhanced angiogenesis of BM-MNC transplantation, and combining CCN1 with BM-MNC transplantation is a useful alternative for ischemic limbs.
authors: Department of Vascular Surgery, Kunming General Hospital, Chengdu Military Command, Kunming, 650032, People's Republic of China, cunping_yin@163.com.
authors: Cunping
authors: C
authors: Yin
authors: Yuan
authors: Y
authors: Liang
authors: Shuguang
authors: S
authors: Guo
authors: Xingli
authors: X
authors: Zhou
authors: Xinghua
authors: X
authors: Pan
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002196:Capillaries / Q000378:metabolism; D002448:Cell Adhesion; D055515:Cysteine-Rich Protein 61 / Q000494:pharmacology*; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods*; D006614:Hindlimb / Q000473:pathology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007511:Ischemia / Q000235:genetics / Q000628:therapy*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: C501849:CCN1 protein, rat; D055515:Cysteine-Rich Protein 61; D011994:Recombinant Proteins