title: Cardiomyogenic differentiation potential of human endothelial progenitor cells isolated from patients with myocardial infarction.
abstract: OBJECTIVE
  Endothelial progenitor cells (EPCs) are known to play a beneficial role by promoting postnatal vasculogenesis in pathological events, such as ischemic heart disease and peripheral artery disease. However, little is known about the potential of EPCs to restore heart damage tissue. We compared the cardiac differentiation capacity of EPCs isolated from peripheral blood of patients with acute myocardial infarction (AMI) with EPCs obtained from umbilical cord blood (UCB).
  
  
  METHODS
  EPCs from both origins were isolated by density gradient centrifugation and characterized through the use of endothelial markers (UEA-1lectin, CD133 and KDR) and endothelial cell colony-forming unit assay. Cardiac differentiation capacity of EPCs was assessed by immunofluorescence and reverse transcriptase-polymerase chain reaction after 5-azacytidine (5-aza) induction.
  
  
  RESULTS
  No significant differences were observed between the number of endothelial cell colony-forming units in peripheral blood of patients with AMI and samples from UCB. Moreover, 5-aza induced the appearance of myotube-like structures and the positive expression of sarcomeric α-actinin, cardiac troponin I and T and desmin in a similar pattern for both cell sources, which indicates a comparable acquisition of a cardiac-like phenotype.
  
  
  CONCLUSIONS
  For the first time, we have compared, in vitro, the cardiomyogenic potential of EPCs derived from patients with AMI with UCB-derived EPCs. Our data indicate that EPCs obtained from both origins have similar plasticity and functions and suggest a potential therapeutic efficacy in cardiac cell therapy.
authors: Department of Health Sciences, University of Jaén, Jaén, Spain.
authors: Elena
authors: E
authors: López-Ruiz
authors: Department of Health Sciences, University of Jaén, Jaén, Spain; Biopathology and Medicine Regenerative Institute (IBIMER), University of Granada, Granada, Spain.
authors: Macarena
authors: M
authors: Perán
authors: Biopathology and Medicine Regenerative Institute (IBIMER), University of Granada, Granada, Spain; Braman Family Breast Cancer Institute, Sylvester Comprehensive Cancer Center, University of Miami, Miller School of Medicine, Miami, Florida, USA.
authors: Manuel
authors: M
authors: Picón-Ruiz
authors: Department of Oncology, Virgen de las Nieves, University Hospital, Granada, Spain; Biosanitary Institute of Granada (ibs.GRANADA), Hospitales Universitarios de Granada-Universidad de Granada, Granada, Spain.
authors: Maria Angel
authors: MA
authors: García
authors: Biopathology and Medicine Regenerative Institute (IBIMER), University of Granada, Granada, Spain; Department of Human Anatomy and Embryology, University of Granada, Granada, Spain.
authors: Esmeralda
authors: E
authors: Carrillo
authors: UGC Corazón, Hospital ClínicoUniversitarioVirgen de la Victoria de Málaga, IBIMA Instituto de Investigación Biomédica de Málaga, Universidad de Málaga, Spain.
authors: Manuel
authors: M
authors: Jiménez-Navarro
authors: Cord Blood Bank, Centro Regional de Transfusión Sanguínea, Málaga, Spain.
authors: M Carmen
authors: MC
authors: Hernández
authors: Cord Blood Bank, Centro Regional de Transfusión Sanguínea, Málaga, Spain.
authors: Isidro
authors: I
authors: Prat
authors: UGC Corazón, Hospital ClínicoUniversitarioVirgen de la Victoria de Málaga, IBIMA Instituto de Investigación Biomédica de Málaga, Universidad de Málaga, Spain.
authors: Eduardo
authors: E
authors: De Teresa
authors: Biopathology and Medicine Regenerative Institute (IBIMER), University of Granada, Granada, Spain; Biosanitary Institute of Granada (ibs.GRANADA), Hospitales Universitarios de Granada-Universidad de Granada, Granada, Spain; Department of Human Anatomy and Embryology, University of Granada, Granada, Spain. Electronic address: jmarchal@ugr.es.
authors: Juan Antonio
authors: JA
authors: Marchal
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D001374:Azacitidine / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001773:Blood Cells / Q000473:pathology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000502:physiology*; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D048091:Guided Tissue Regeneration / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy*; D032383:Myocytes, Cardiac / Q000502:physiology*; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D015415:Biomarkers; D001374:Azacitidine
keywords: acute myocardial infarction; cardiomyocyte differentiation; endothelial progenitor cells; umbilical cord blood