title: Analysis of S-locus and expression of S-alleles of self-compatible rapid-cycling Brassica oleracea 'TO1000DH3'.
abstract: Brassica oleracea is a strictly self-incompatible (SI) plant, but rapid-cycling B. oleracea 'TO1000DH3' is self-compatible (SC). Self-incompatibility in Brassicaceae is controlled by multiple alleles of the S-locus. Three S-locus genes, S-locus glycoprotein (SLG), S-locus receptor kinase (SRK) and S-locus protein 11 or S-locus cysteine-rich (SP11/SCR), have been reported to date, all of which are classified into class I and II. In this study, we investigated the molecular mechanism behind alterations of SI to SC in rapid-cycling B. olerace 'TO1000DH3'. Class I SRK were identified by genomic DNA PCR and PCR-RFLP analysis using SRK specific markers and found to be homozygous. Cloning and sequencing of class I SRK revealed a normal kinase domain without any S-domain/transmembrane domain. Moreover, S-locus sequencing analysis revealed only an SLG sequence, but no SP11/SCR. Expression analysis showed no SRK expression in the stigma, although other genes involved in the SI recognition reaction (SLG, MLPK, ARC1, THL) were found to have normal expression in the stigma. Taken together, the above results suggest that structural aberrations such as deletion of the SI recognition genes may be responsible for the breakdown of SI in rapid-cycling B. oleracea 'TO1000DH3'.
authors: Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam, 540-742, Republic of Korea.
authors: Jung
authors: J
authors: Hee-Jeong
authors: Ahmed
authors: A
authors: Nasar Uddin
authors: Park
authors: P
authors: Jong-In
authors: Thamilarasan
authors: T
authors: Senthil Kumar
authors: Kim
authors: K
authors: Hye-Ran
authors: Cho
authors: C
authors: Yong-Gu
authors: Nou
authors: N
authors: Ill-Sup
mesh_terms: D000483:Alleles; D001937:Brassica / Q000235:genetics*; D015870:Gene Expression; D023061:Gene Order; D017343:Genes, Plant; D006239:Haplotypes; D020161:Physical Chromosome Mapping; D054817:Pollination / Q000235:genetics; D040641:Quantitative Trait Loci