title: Preliminary investigation of the dissolution behavior, cytocompatibility, effects of fibrinogen conformation and platelet adhesion for radiopaque embolic particles.
abstract: Experimental embolic particles based on a novel zinc-silicate glass system have been biologically evaluated for potential consideration in transcatheter arterial embolization procedures. In addition to controlling the cytotoxicity and haemocompatibility for such embolic particles, its glass structure may mediate specific responses via dissolution in the physiological environment. In a 120 h in-vitro dissolution study, ion release levels for silicon (Si4+), sodium (Na+), calcium (Ca2+), zinc (Zn2+), titanium (Ti4+), lanthanum (La3+), strontium (Sr2+), and magnesium (Mg2+), were found to range from 0.04 to 5.41 ppm, 0.27-2.28 ppm, 2.32-8.47 ppm, 0.16-0.20 ppm, 0.12-2.15 ppm, 0.16-0.49 ppm and 0.01-0.12 ppm, respectively for the series of glass compositions evaluated. Initial release of Zn2+ (1.93-10.40 ppm) was only evident after 120 h. All compositions showed levels of cell viabilities ranging from 61.31 ± 4.33% to 153.7 ± 1.25% at 25%-100% serial extract dilutions. The conformational state of fibrinogen, known to induce thrombi, indicated that no changes were induced with respect of the materials dissolution by-products. Furthermore, the best-in-class experimental composition showed equivalency to contour PVA in terms of inducing platelet adhesion. The data generated here provides requisite evidence to continue to in-vivo pre-clinical evaluation using the best-in-class experimental composition evaluated.
authors: Department of Applied Oral Sciences, Dalhousie University, PO Box 15000, Halifax, NS B3H 4R2, Canada. sharonkehoe@dal.ca.
authors: Sharon
authors: S
authors: Kehoe
authors: Department of Biochemistry & Molecular Biology, Dalhousie University, NS, B3H 4R2, Canada. mltremblay@dal.ca.
authors: Marie-Laurence
authors: ML
authors: Tremblay
authors: Inamori School of Engineering, Alfred University, Alfred, NY 14802, USA. coughlan@alfred.edu.
authors: Aisling
authors: A
authors: Coughlan
authors: Faculty of Engineering & Architectural Science, Ryerson University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada. mtowler@ryerson.ca.
authors: Mark R
authors: MR
authors: Towler
authors: Department of Biochemistry & Molecular Biology, Dalhousie University, NS, B3H 4R2, Canada. jan.rainey@dal.ca.
authors: Jan K
authors: JK
authors: Rainey
authors: Department of Diagnostic Imaging and Interventional Radiology, QE II Health Sciences Centre, Dalhousie University, Halifax, NS B3H 4R2, Canada. rjabrahamresearch@gmail.com.
authors: Robert J
authors: RJ
authors: Abraham
authors: Department of Applied Oral Sciences, Dalhousie University, PO Box 15000, Halifax, NS B3H 4R2, Canada. d.boyd@dal.ca.
authors: Daniel
authors: D
authors: Boyd