title: MicroRNA-218 inhibits the proliferation of human choriocarcinoma JEG-3 cell line by targeting Fbxw8.
abstract: MicroRNAs (miRNAs) are endogenous 19-25 nucleotide noncoding single-stranded RNAs that regulate gene expression by blocking the translation or decreasing the stability of mRNAs. In this study, we showed that miR-218 expression levels were decreased while Fbxw8 expression levels were increased in human choriocarcinoma cell lines, and identified Fbxw8 as a novel direct target of miR-218. Overexpression of miR-218 inhibited cell growth arrest at G2/M phase, suppressed the protein levels of cyclin A and up-regulated the expression levels of p27 through decreasing the levels of Fbxw8. On the other hand, forced expression of Fbxw8 partly rescued the effect of miR-218 in the cells, attenuated cell proliferation decrease the percentage of cells at G2/M phase, induced cyclin A protein expression and suppressed the protein level of p27 through up-regulating the levels of Fbxw8. Taken together, these findings will shed light the role to mechanism of miR-218 in regulating JEG-3 cells proliferation via miR-218/Fbxw8 axis, and miR-218 may serve as a novel potential therapeutic target in human choriocarcinoma in the future.
authors: Department of Obstetric and Gynaecology, Xiangya Hospital, Xiangya Hospital of Central South University, Changsha, China.
authors: Dazun
authors: D
authors: Shi
authors: Department of Obstetric and Gynaecology, Xiangya Hospital, Xiangya Hospital of Central South University, Changsha, China.
authors: Zhihui
authors: Z
authors: Tan
authors: Department of Obstetric and Gynaecology, Xiangya Hospital, Xiangya Hospital of Central South University, Changsha, China.
authors: Rong
authors: R
authors: Lu
authors: Department of Obstetric and Gynaecology, Xiangya Hospital, Xiangya Hospital of Central South University, Changsha, China.
authors: Wenqing
authors: W
authors: Yang
authors: Department of Obstetric and Gynaecology, Xiangya Hospital, Xiangya Hospital of Central South University, Changsha, China. Electronic address: zhangyi5588@hotmail.com.
authors: Yi
authors: Y
authors: Zhang
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002822:Choriocarcinoma / Q000378:metabolism / Q000473:pathology*; D044783:F-Box Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D018797:Cell Cycle Proteins; D044783:F-Box Proteins; C526069:FBXW8 protein, human; C547471:MIRN218 microRNA, human; D035683:MicroRNAs
keywords: Fbxw8; JEG-3; Proliferation; miR-218