title: Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex.
abstract: A novel collagenolytic serine protease was identified and then purified (along with ficin) to apparent homogeneity from the latex of fig (Ficus carica, var. Brown Turkey) by two step chromatographic procedure using gel and covalent chromatography. The enzyme is a monomeric protein of molecular mass of 41 ± 9 kDa as estimated by analytical gel filtration chromatography. It is an acidic protein with a pI value of approximately 5 and optimal activity at pH 8.0-8.5 and temperature 60°C. The enzymatic activity was strongly inhibited by PMSF and Pefabloc SC, indicating that the enzyme is a serine protease. The enzyme showed specificity towards gelatin and collagen (215 GDU/mg and 24.8 CDU/mg, respectively) and non-specific protease activity (0.18 U/mg against casein). The enzyme was stable and retained full activity over a broad range of pH and temperature. The fig latex collagenolytic protease is potentially useful as a non-microbial enzyme with collagenolytic activity for various applications in the fields of biochemistry, biotechnology and medicine.
authors: University of Belgrade, Faculty of Chemistry, Department of Biochemistry, Studentski trg 12 - 16, 11000 Belgrade, Republic of Serbia.
authors: Brankica
authors: B
authors: Raskovic
authors: University of Belgrade, Faculty of Chemistry, Department of Biochemistry, Studentski trg 12 - 16, 11000 Belgrade, Republic of Serbia.
authors: Olga
authors: O
authors: Bozovic
authors: University of Belgrade, Faculty of Chemistry, Department of Biochemistry, Studentski trg 12 - 16, 11000 Belgrade, Republic of Serbia.
authors: Radivoje
authors: R
authors: Prodanovic
authors: University of Belgrade, Faculty of Chemistry, Department of Biochemistry, Studentski trg 12 - 16, 11000 Belgrade, Republic of Serbia.
authors: Vesna
authors: V
authors: Niketic
authors: University of Belgrade, Faculty of Chemistry, Department of Biochemistry, Studentski trg 12 - 16, 11000 Belgrade, Republic of Serbia. Electronic address: polovicn@chem.bg.ac.rs.
authors: Natalija
authors: N
authors: Polovic
mesh_terms: D002364:Caseins / Q000378:metabolism; D002850:Chromatography, Gel; D003094:Collagen / Q000378:metabolism*; D004795:Enzyme Stability; D030681:Ficus / Q000201:enzymology*; D005780:Gelatin / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007840:Latex / Q000737:chemistry*; D008970:Molecular Weight; D057057:Serine Proteases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D002364:Caseins; D007840:Latex; D015842:Serine Proteinase Inhibitors; D005780:Gelatin; D003094:Collagen; D057057:Serine Proteases
keywords: Collagenolytic activity; Enzyme purification; Fig latex; Gelatinolytic activity; Serine protease