title: Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes.
abstract: Silene vulgaris possesses ecotype-specific tolerance to high levels of copper in the soil. Although this was reported a few decades ago, little is known about this trait on a molecular level. The aim of this study was to analyze the transcription response to elevated copper concentrations in two S. vulgaris ecotypes originating from copper-contrasting soil types - copper-tolerant Lubietova and copper-sensitive Stranska skala. To reveal if plants are transcriptionally affected, we first analyzed the HMA7 gene, a known key player in copper metabolism. Based on BAC library screening, we identified a BAC clone containing a SvHMA7 sequence with all the structural properties specific for plant copper-transporting ATPases. The functionality of the gene was tested using heterologous complementation in yeast mutants. Analyses of SvHMA7 transcription patterns showed that both ecotypes studied up-regulated SvHMA7 transcription after the copper treatment. Our data are supported by analysis of appropriate reference genes based on RNA-Seq databases. To identify genes specifically involved in copper response in the studied ecotypes, we analyzed transcription profiles of genes coding Cu-transporting proteins and genes involved in the prevention of copper-induced oxidative stress in both ecotypes. Our data show that three genes (APx, POD and COPT5) differ in their transcription pattern between the ecotypes with constitutively increased transcription in Lubietova. Taken together, we have identified transcription differences between metallifferous and non-metalliferous ecotypes of S. vulgaris, and we have suggested candidate genes participating in metal tolerance in this species.
authors: Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic. Electronic address: baloun@ibp.cz.
authors: Jiri
authors: J
authors: Baloun
authors: Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic; Department of Plant Biology, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
authors: Eva
authors: E
authors: Nevrtalova
authors: Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic.
authors: Viera
authors: V
authors: Kovacova
authors: Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic.
authors: Vojtech
authors: V
authors: Hudzieczek
authors: Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic.
authors: Radim
authors: R
authors: Cegan
authors: Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic.
authors: Boris
authors: B
authors: Vyskot
authors: Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic; Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Slechtitelu 31, CZ-78371 Olomouc, Czech Republic.
authors: Roman
authors: R
authors: Hobza
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D000073840:Copper-Transporting ATPases; D030561:Databases, Nucleic Acid; D060146:Ecotype; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D009928:Organ Specificity; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics; D017423:Sequence Analysis, RNA; D029751:Silene / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D059467:Transcriptome
chemical_list: D027682:Cation Transport Proteins; D010940:Plant Proteins; D018749:RNA, Plant; D003300:Copper; D000251:Adenosine Triphosphatases; D000073840:Copper-Transporting ATPases
keywords: Copper; Genes coding ROS-eliminating and Cu-transporting proteins; RNA-Seq database; Tissue-specific transcription