title: Gut microbe analysis between hyperthyroid and healthy individuals.
abstract: Clinicians have long recognized that thyroid hormones have some effects on the gastrointestinal tract. This study aimed to investigate the gut microbiota in hyperthyroid and assess whether there are alterations in the diversity and similarity of gut microbiota in the hyperthyroid when compared with healthy individuals. PCR-denaturing gradient gel electrophoresis (DGGE) with universal primers targeting V3 region of the 16S rRNA gene was employed to characterize the overall intestinal microbiota composition, and some excised gel bands were cloned for sequencing. Enterobacteriaceae, Enterococcus, Bifidobacterium, Clostridium, and Lactobacillus genus were also enumerated by quantitative real-time PCR. A significant difference between hyperthyroid and healthy groups ((*) P < 0.05) was shown in DGGE profiles. And real-time PCR showed obvious decrease of Bifidobacterium and Lactobacillus ((*) P < 0.05), and increase of Enterococcus ((*) P < 0.05) in the hyperthyroid group. This study shows the characterization of gut microbiota in hyperthyroid.
authors: Department of Biotechnology, Dalian Medical University, 9 Western Section, Lvshun South Street, Dalian, People's Republic of China.
authors: Lei
authors: L
authors: Zhou
authors: Xinli
authors: X
authors: Li
authors: Ayaz
authors: A
authors: Ahmed
authors: Dachang
authors: D
authors: Wu
authors: Liang
authors: L
authors: Liu
authors: Juanjuan
authors: J
authors: Qiu
authors: Yao
authors: Y
authors: Yan
authors: Meilan
authors: M
authors: Jin
authors: Yi
authors: Y
authors: Xin
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D058491:Bacterial Load; D058448:Biota; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D041981:Gastrointestinal Tract / Q000382:microbiology*; D064368:Healthy Volunteers; D006801:Humans; D006980:Hyperthyroidism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S