title: Gold nanoparticle-packed microdisks for multiplex Raman labelling of cells.
abstract: Micro/nanoparticles containing densely packed gold nanoparticles (AuNPs) possess unique properties potentially useful for various biomedical applications. The micro/nanoparticles are conventionally produced by the bottom-up methods, which have limited capability for controlling the particle size, shape and structure. This article reports development of a top-down method that integrates layer-by-layer assembly and microcontact printing to fabricate disk-shaped microparticles named microdisks composed of densely packed AuNPs. This method allows precise control of not only the size, shape and structure of the microdisks but also the amount of the AuNPs in the microdisks. The microdisks can be loaded with different Raman reporters to generate characteristic surface-enhanced Raman scattering spectra under the near infrared excitation over a centimetre-scale lens-sample distance. Moreover, the microdisks can be attached to single live cells. This microdisk platform holds potential for multiplex Raman labelling of therapeutic cells for in vivo tracking of the cells.
authors: Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer Street, Tallahassee, Florida 32310, USA. guan@eng.fsu.edu.
authors: Peipei
authors: P
authors: Zhang
authors: Junfei
authors: J
authors: Xia
authors: Zhibin
authors: Z
authors: Wang
authors: Jingjiao
authors: J
authors: Guan
mesh_terms: D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008863:Microspheres; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011142:Polyvinyl Alcohol / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D006046:Gold; D011142:Polyvinyl Alcohol; D012825:Silicon