title: Immobilization as a strategy for improving enzyme properties-application to oxidoreductases.
abstract: The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properties of enzymes such as performance in organic solvents, pH tolerance, heat stability or the functional stability. Increasing the structural rigidity of the protein and stabilization of multimeric enzymes which prevents dissociation-related inactivation. In the last decade, several papers about immobilization methods have been published. In our work, we present a relation between the influence of immobilization on the improvement of the properties of selected oxidoreductases and their commercial value. We also present our view on the role that different immobilization methods play in the reduction of enzyme inhibition during biotechnological processes.
authors: University of Silesia in Katowice, Faculty of Biology and Environmental Protection, Department of Biochemistry, Jagiellonska 28, 40-032 Katowice, Poland. urszula.guzik@us.edu.pl.
authors: Urszula
authors: U
authors: Guzik
authors: University of Silesia in Katowice, Faculty of Biology and Environmental Protection, Department of Biochemistry, Jagiellonska 28, 40-032 Katowice, Poland. katarzyna.hupert-kocurek@us.edu.pl.
authors: Katarzyna
authors: K
authors: Hupert-Kocurek
authors: University of Silesia in Katowice, Faculty of Biology and Environmental Protection, Department of Biochemistry, Jagiellonska 28, 40-032 Katowice, Poland. danuta.wojcieszynska@us.edu.pl.
authors: Danuta
authors: D
authors: Wojcieszyńska
mesh_terms: D000818:Animals; D055162:Biocatalysis; D001673:Biodegradation, Environmental; D001709:Biotechnology; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006801:Humans; D010088:Oxidoreductases / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D010088:Oxidoreductases