title: Pharmacokinetic study of multiple active constituents from Kushen-Gancao Decoction after oral administration in rat by HPLC-MS/MS.
abstract: Kushen-Gancao Decoction (KGD) is a classic traditional Chinese herb combination in treating viral hepatitis and chronic liver diseases. This study aims to investigate the pharmacokinetic (PK) study of matrine (MT), oxymatrine (OMT), glycyrrhizic acid (GL) and glycyrrhetinic acid (GA) following oral administration of KGD in rats. A rapid, sensitive and reliable HPLC-MS/MS method was successfully developed for the simultaneous determination of MT, OMT, GL and GA in rat plasma. A Inertsil C18 analytical column was used with a gradient mobile phase system of methanol-ammonium acetate (5mM) with a flow rate of 0.5 mL/min. The analysis was performed on a positive and negative ionization electrospray mass spectrometer via multi reaction monitoring (MRM). Linear calibration curves were obtained for the following concentration range: 10-5000 ng/mL for MT, OMT and GL, 50-15,000 ng/mL for GA in rat plasma (R(2)>0.99). The lower limit of quantification (LLOQ) was 5 ng/mL (MT, OMT and GL) and 20 ng/mL (GA). The intra- and inter-day accuracies ranged from -7.91 to 9.10% and precisions (RSD) were within 15%. The analytes were found to be stable under short-term temperature conditions, post-preparative temperature conditions, and after three freeze-thaw cycles conditions. The validated method was successfully applied to a pharmacokinetic study in rats after oral administration of KGD.
authors: Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China.
authors: Qinhui
authors: Q
authors: Wang
authors: Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China.
authors: Lei
authors: L
authors: Shi
authors: Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China.
authors: Xiuling
authors: X
authors: Tang
authors: Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China.
authors: Qingwei
authors: Q
authors: Wang
authors: Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China.
authors: Xueliang
authors: X
authors: Dang
authors: Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China. Electronic address: QinHuiWangTDYY@Gmail.com.
authors: Yan
authors: Y
authors: Zhang
mesh_terms: D000284:Administration, Oral; D000470:Alkaloids / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D006034:Glycyrrhetinic Acid / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D019695:Glycyrrhizic Acid / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D011807:Quinolizines / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; D019695:Glycyrrhizic Acid; C037573:oxymatrine; D006034:Glycyrrhetinic Acid; D000093842:Matrines
keywords: Glycyrrhetinic acid; Glycyrrhizic acid; HPLC–MS/MS; Matrine; Oxymatrine; Pharmacokinetic