title: Vidas UP-enzyme-linked fluorescent immunoassay based on recombinant phage protein and fluorescence in situ hybridization as alternative methods for detection of Salmonella enterica serovars in meat.
abstract: Several methods for the rapid and specific detection of Salmonella spp. in meat have been described. This study was conducted to evaluate the capability of the VIDAS(®) UP (SPT [Salmonella Phage Technology]), an enzyme-linked fluorescent immunoassay method, and fluorescence in situ hybridization (FISH) to complement the International Organization for Standardization Method 6579 (ISO) in detecting Salmonella spp. from beef, pork, and poultry meat samples. The meat was inoculated with a mixture of Salmonella spp. on three levels of contamination. It was also checked that the tests did not produce cross-reactions with other Enterobacteriaceae rods. On the basis of the results, the relative specificity, relative accordance, and relative sensitivity of the method were determined. In meat samples, Vidas UP and FISH detection results were in substantial agreement with ISO, with relative specificity, accordance, and sensitivity rates of 90%, 96.3%, and 100%, respectively, for Vidas UP and 100%, 100%, and 99.4%, respectively, for FISH. This is the first report on the evaluation of both Vidas UP and FISH compared to ISO for the rapid detection of Salmonella enterica serovars in meat.
authors: Department of Industrial and Food Microbiology, Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn , Olsztyn, Poland .
authors: Anna
authors: A
authors: Zadernowska
authors: Wioleta
authors: W
authors: Chajęcka-Wierzchowska
authors: Lucyna
authors: L
authors: Kłębukowska
mesh_terms: D000818:Animals; D057205:Automation, Laboratory; D001435:Bacteriophages; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008460:Meat / Q000382:microbiology*; D011994:Recombinant Proteins; D015203:Reproducibility of Results; D019779:Salmonella enterica / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D013552:Swine; D014764:Viral Proteins
chemical_list: D011994:Recombinant Proteins; D014764:Viral Proteins