title: Human hematopoietic reconstitution and HLA-restricted responses in nonpermissive alymphoid mice.
abstract: We generated a new humanized mouse model to study HLA-restricted immune responses. For this purpose, we created unique murine hosts by enforcing the expression of human SIRPα by murine phagocytes in murine MHC-deficient HLA-transgenic alymphoid hosts, an approach that allowed the immune reconstitution of nonpermissive mice following injection of human hematopoietic stem cells. We showed that these mouse/human chimeras were able to generate HLA-restricted responses to immunization. These new humanized mice may offer attractive models to study immune responses to human diseases, such as HIV and EBV infections, as well as to assay new vaccine strategies.
authors: Unité de Biologie des Populations Lymphocytaires, Département d'Immunologie, Institut Pasteur, 75724 Paris, France; Centre National pour la Recherche Scientifique, Unité de Recherche Associée 1961, 75724 Paris, France;
authors: Malika
authors: M
authors: Serra-Hassoun
authors: Laboratoire de Pathogenèse des Virus de l'Hépatite B, Département de Virologie, Institut Pasteur, 75724 Paris, France; INSERM U845, 75724 Paris, France;
authors: Maryline
authors: M
authors: Bourgine
authors: Unit of Human Evolutionary Genetics, Department of Genome and Genetics, Institut Pasteur, 75724 Paris, France; and.
authors: Michele
authors: M
authors: Boniotto
authors: Unité de Biologie des Populations Lymphocytaires, Département d'Immunologie, Institut Pasteur, 75724 Paris, France; Centre National pour la Recherche Scientifique, Unité de Recherche Associée 1961, 75724 Paris, France;
authors: Julien
authors: J
authors: Berges
authors: Centre d'Ingénierie Génétique Murine, Institut Pasteur, 75724 Paris, France.
authors: Francina
authors: F
authors: Langa
authors: Laboratoire de Pathogenèse des Virus de l'Hépatite B, Département de Virologie, Institut Pasteur, 75724 Paris, France; INSERM U845, 75724 Paris, France;
authors: Marie-Louise
authors: ML
authors: Michel
authors: Unité de Biologie des Populations Lymphocytaires, Département d'Immunologie, Institut Pasteur, 75724 Paris, France; Centre National pour la Recherche Scientifique, Unité de Recherche Associée 1961, 75724 Paris, France;
authors: Antonio A
authors: AA
authors: Freitas
authors: Unité de Biologie des Populations Lymphocytaires, Département d'Immunologie, Institut Pasteur, 75724 Paris, France; Centre National pour la Recherche Scientifique, Unité de Recherche Associée 1961, 75724 Paris, France; sylvie.garcia@pasteur.fr.
authors: Sylvie
authors: S
authors: Garcia
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000943:Antigens, Differentiation / Q000008:administration & dosage / Q000097:blood / Q000235:genetics; D002470:Cell Survival / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006680:HLA Antigens / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D008822:Mice, Transgenic; D009924:Organ Culture Techniques; D011828:Radiation Chimera / Q000235:genetics / Q000276:immunology*; D011971:Receptors, Immunologic / Q000008:administration & dosage / Q000097:blood / Q000235:genetics
chemical_list: D000943:Antigens, Differentiation; D006680:HLA Antigens; D011971:Receptors, Immunologic; C501920:SIRPA protein, human