title: Periodontal ligament-derived cells for periodontal regeneration in animal models: a systematic review.
abstract: OBJECTIVE
  Implantation of periodontal ligament stem cells is emerging as a potential periodontal regenerative procedure. This systematic review considers the evidence from animal models investigating the use of periodontal ligament stem cells for successful periodontal regeneration.
  
  
  METHODS
  PubMed, Embase, MEDLINE and Google Scholar were searched to December 2013 for quantitative studies examining the outcome of implanting periodontal ligament stem cells into experimental periodontal defects in animals. Inclusion criteria were: implantation of periodontal ligament stem cells into surgically created periodontal defects for periodontal regeneration; animal models only; source of cells either human or animal; and published in English. We followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines.
  
  
  RESULTS
  From the literature search, 43 studies met the inclusion criteria. A wide variety of surgical defects were created in four species of animal (dog, rat, pig and sheep). Owing to wide variability in defect type, cell source and cell scaffold, no meta-analysis was possible. Outcome measures included new bone, new cementum and new connective tissue formation. In 70.5% of the results, statistically significant improvements of these measures was recorded.
  
  
  CONCLUSIONS
  These results are notable in that they indicate that irrespective of the defect type and animal model used, periodontal ligament stem cell implantation can be expected to result in a beneficial outcome for periodontal regeneration. It is recommended that there is sufficient evidence from preclinical animal studies to warrant moving to human studies to examine the efficacy, safety, feasibility (autologous vs. allogeneic transplantation) and delivery of periodontal ligament stem cells for periodontal regeneration.
authors: Colgate Australian Clinical Dental Research, School of Dentistry, University of Adelaide, Adelaide, South Australia, Australia.
authors: R
authors: R
authors: Bright
authors: K
authors: K
authors: Hynes
authors: S
authors: S
authors: Gronthos
authors: P M
authors: PM
authors: Bartold
mesh_terms: D000818:Animals; D025021:Cementogenesis / Q000502:physiology; D004195:Disease Models, Animal; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D006801:Humans; D010012:Osteogenesis / Q000502:physiology; D010510:Periodontal Diseases / Q000628:therapy; D010513:Periodontal Ligament / Q000166:cytology*; D012038:Regeneration / Q000502:physiology; D013234:Stem Cells / Q000502:physiology*
keywords: animal models; periodontal regeneration; stem cells; systematic review