title: [Effect of thymic stromal lymphopoietin on human bronchial epithelial permeability].
abstract: OBJECTIVE
  To investigate the effect of thymic stromal lymphopoietin (TSLP) on the permeablily of monolayer bronchial epithelial cells in vitro.
  
  
  METHODS
  Cultured human bronchial epithelial cell line 16HBE was exposed to 0.1 or 1 ng/ml TSLP for 0, 0.5, 6, 12, or 24 h, and the epithelial monolayer permeability was assessed by measuring transepithelial electrical resistance (TER), permeability to FITC-labeled dextran (FITC-DX) and expression of E-cadherin.
  
  
  RESULTS
  Compared with the control cells group, 16HBE cell monolayer showed significantly increased TER (P<0.001) and decreased FITC-DX fluorescence in the lower chamber (P<0.05) following exposure to 0.1 and 1 ng/ml TSLP, but these changes were not dose-dependent. Exposure to 0.1 ng/ml TSLP resulted in significantly increased expression of E-cadherin. The 16HBE monolayer exposed to 0.1 ng/ml TSLP for 24 h showed the most obvious increase of TER and E-cadherin expression (P<0.05); FITC-DX fluorescence level was markedly decreased after TSLP exposure for 12 h and 24 h (P<0.05), and the effect was more obvious in 12 h group.
  
  
  CONCLUSIONS
  TSLP can protect the barrier function of normal bronchial epithelial cells in vitro.
authors: Department of Respiratory and Critical Care Medicine, Chronic Airway Diseases Laboratory, Nanfang Hospitial, Southern Medical University, Guangzhou 510515, China.E-mail:articlewenjiali@126.com.
authors: Wenjia
authors: W
authors: Li
authors: Haijin
authors: H
authors: Zhao
authors: Hangming
authors: H
authors: Dong
authors: Fei
authors: F
authors: Zou
authors: Shaoxi
authors: S
authors: Cai
mesh_terms: D015703:Antigens, CD; D001980:Bronchi / Q000166:cytology*; D015820:Cadherins / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects*; D006801:Humans; D010539:Permeability; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D016207:Cytokines; D000094632:Thymic Stromal Lymphopoietin