title: Miniaturized bronchoscopy enables unilateral investigation, application, and sampling in mice.
abstract: Lung diseases, including pneumonia and asthma, are among the most prevalent human disorders, and murine models have been established to investigate their pathobiology and develop novel treatment approaches. Whereas bronchoscopy is valuable for diagnostic and therapeutic procedures in patients, no equivalent for small rodents has been established. Here, we introduce a miniaturized video-bronchoscopy system offering new opportunities in experimental lung research. With an outer diameter of 0.75 mm, it is possible to advance the optics into the main bronchi of mice. An irrigation channel allows bronchoalveolar lavage and unilateral application of substances to one lung. Even a unilateral infection is possible, enabling researchers to use the contralateral lung as internal control.
authors: 1 Institute of Medical Immunology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors: Claudia
authors: C
authors: Dames
authors: Levent
authors: L
authors: Akyüz
authors: Katrin
authors: K
authors: Reppe
authors: Christoph
authors: C
authors: Tabeling
authors: Kristina
authors: K
authors: Dietert
authors: Olivia
authors: O
authors: Kershaw
authors: Achim D
authors: AD
authors: Gruber
authors: Christian
authors: C
authors: Meisel
authors: Andreas
authors: A
authors: Meisel
authors: Martin
authors: M
authors: Witzenrath
authors: Odilo
authors: O
authors: Engel
mesh_terms: D000818:Animals; D020680:Bronchoscopes; D001999:Bronchoscopy / Q000379:methods; D004195:Disease Models, Animal; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D016215:Macrophage-Activating Factors / Q000494:pharmacology; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000276:immunology; D011018:Pneumonia, Pneumococcal / Q000175:diagnosis / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology
chemical_list: D016215:Macrophage-Activating Factors
keywords: bronchoscopy; mouse; unilateral application in lung