title: Oxidative stress is related to frailty, not to age or sex, in a geriatric population: lipid and protein oxidation as biomarkers of frailty.
abstract: OBJECTIVE
  To ascertain whether indicators of oxidative damage to lipids (malondialdehyde (MDA)) and proteins (protein carbonylation) are biomarkers of frailty, after adjusting for age, sex, and other possible confounders.
  
  
  METHODS
  Cross-sectional cohort study.
  
  
  METHODS
  Community.
  
  
  METHODS
  Toledo Study for Healthy Aging participants (N = 742, aged 65-95), classified as frail (n = 54), prefrail (n = 278) and nonfrail (n = 410) according to the Fried criteria.
  
  
  METHODS
  Blood plasma was obtained using centrifugation (1,500 G, 15 minutes) and immediately frozen at -80°C. Plasma lipid peroxidation was determined by measuring the MDA formed from lipoperoxides using high-performance liquid chromatography and protein carbonylation was measured using Western blot.
  
  
  RESULTS
  Age- and sex-adjusted levels of lipoperoxides (measured as MDA) and protein carbonylation in plasma proved to be related to frailty, even after including possible independent confounders.
  
  
  CONCLUSIONS
  Circulating oxidative damage biomarkers, such as MDA and protein carbonylation, are related to frailty and not to age or sex. These parameters may be considered as potential biomarkers of frailty in the context of a multidisciplinary health-promoting approach for older adults.
authors: Department of Physiotherapy, Faculty of Physiotherapy, University of Valencia, Valencia, Spain.
authors: Marta
authors: M
authors: Inglés
authors: Juan
authors: J
authors: Gambini
authors: Jose A
authors: JA
authors: Carnicero
authors: Francisco J
authors: FJ
authors: García-García
authors: Leocadio
authors: L
authors: Rodríguez-Mañas
authors: Gloria
authors: G
authors: Olaso-González
authors: Mar
authors: M
authors: Dromant
authors: Consuelo
authors: C
authors: Borrás
authors: Jose
authors: J
authors: Viña
mesh_terms: D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D005260:Female; D016330:Frail Elderly; D006801:Humans; D050356:Lipid Metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D011506:Proteins / Q000378:metabolism*; D012737:Sex Factors
chemical_list: D015415:Biomarkers; D011506:Proteins; D008315:Malondialdehyde
keywords: biomarkers; elderly; frailty; oxidative damage