title: Redox and nitric oxide homeostasis are affected in tomato (Solanum lycopersicum) roots under salinity-induced oxidative stress.
abstract: The nicotinamide adenine dinucleotide phosphate (NADPH) and reduced glutathione (GSH) molecules play important roles in the redox homeostasis of plant cells. Using tomato (Solanum lycopersicum) plants grown with 120mM NaCl, we studied the redox state of NADPH and GSH as well as ascorbate, nitric oxide (NO) and S-nitrosoglutathione (GSNO) content and the activity of the principal enzymes involved in the metabolism of these molecules in roots. Salinity caused a significant reduction in growth parameters and an increase in oxidative parameters such as lipid peroxidation and protein oxidation. Salinity also led to an overall decrease in the content of these redox molecules and in the enzymatic activities of the main NADPH-generating dehydrogenases, S-nitrosoglutathione reductase and catalase. However, NO content as well as gluthahione reductase and glutathione peroxidase activity increased under salinity stress. These findings indicate that salinity drastically affects redox and NO homeostasis in tomato roots. In our view, these molecules, which show the interaction between ROS and RNS metabolisms, could be excellent parameters for evaluating the physiological conditions of plants under adverse stress conditions.
authors: Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080 Granada, Spain; Faculty of Sciences of Tunisia, University Tunis El Manar, Tunis, Tunisia.
authors: Jamel
authors: J
authors: Manai
authors: Faculty of Sciences of Tunisia, University Tunis El Manar, Tunis, Tunisia.
authors: Houda
authors: H
authors: Gouia
authors: Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080 Granada, Spain. Electronic address: javier.corpas@eez.csic.es.
authors: Francisco J
authors: FJ
authors: Corpas
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D018551:Solanum lycopersicum / Q000378:metabolism* / Q000502:physiology; D009249:NADP / Q000378:metabolism; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010641:Phenotype; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D009569:Nitric Oxide; D012965:Sodium Chloride; D009249:NADP; D009252:NADPH Dehydrogenase; D005978:Glutathione; D001205:Ascorbic Acid
keywords: GSH; NADP-dehydrogenase; Nitric oxide; S-nitrosoglutathione; Salinity