title: Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4.
abstract: The small ubiquitin-like modifier (SUMO) can form polymeric chains that are important signals in cellular processes such as meiosis, genome maintenance and stress response. The SUMO-targeted ubiquitin ligase RNF4 engages with SUMO chains on linked substrates and catalyses their ubiquitination, which targets substrates for proteasomal degradation. Here we use a segmental labelling approach combined with solution nuclear magnetic resonance (NMR) spectroscopy and biochemical characterization to reveal how RNF4 manipulates the conformation of the SUMO chain, thereby facilitating optimal delivery of the distal SUMO domain for ubiquitin transfer.
authors: 1] Centre for Structural Biology, Department of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK [2].
authors: Yingqi
authors: Y
authors: Xu
authors: 1] Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK [2].
authors: Anna
authors: A
authors: Plechanovová
authors: Centre for Structural Biology, Department of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK.
authors: Peter
authors: P
authors: Simpson
authors: Centre for Structural Biology, Department of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK.
authors: Jan
authors: J
authors: Marchant
authors: Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.
authors: Orsolya
authors: O
authors: Leidecker
authors: Centre for Structural Biology, Department of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK.
authors: Sebastian
authors: S
authors: Kraatz
authors: Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.
authors: Ronald T
authors: RT
authors: Hay
authors: Centre for Structural Biology, Department of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK.
authors: Steve J
authors: SJ
authors: Matthews
mesh_terms: D020816:Amino Acid Motifs; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D025941:Protein Interaction Mapping; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D054875:Ubiquitination / Q000502:physiology*
chemical_list: D009687:Nuclear Proteins; C114070:RNF4 protein, human; C117909:SUMO2 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D014157:Transcription Factors; D046988:Proteasome Endopeptidase Complex