title: Prolonged nitric oxide exposure enhances anoikis resistance and migration through epithelial-mesenchymal transition and caveolin-1 upregulation.
abstract: Nitric oxide (NO) in tumor microenvironment may have a significant impact on metastatic behaviors of cancer. Noncytotoxic doses of NO enhanced anoikis resistance and migration in lung cancer H23 cells via an increase in lamellipodia, epithelial-mesenchymal transition (EMT) markers including vimentin and snail, and caveolin-1 (Cav-1). However, the induction of EMT was found in Cav-1-knock down cells treated with NO, suggesting that EMT was through Cav-1-independent pathway. These effects of NO were consistently observed in other lung cancer cells including H292 and H460 cells. These findings highlight the novel role of NO on EMT and metastatic behaviors of cancer cells.
authors: Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences and Cell-Based Drug and Health Products Development Research Unit, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
authors: Pithi
authors: P
authors: Chanvorachote
authors: Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences and Cell-Based Drug and Health Products Development Research Unit, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
authors: Varisa
authors: V
authors: Pongrakhananon
authors: Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences and Cell-Based Drug and Health Products Development Research Unit, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
authors: Preedakorn
authors: P
authors: Chunhacha
mesh_terms: D023102:Anoikis; D051242:Caveolin 1 / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009569:Nitric Oxide / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D009363:Neoplasm Proteins; D009569:Nitric Oxide