title: Antioxidant activity profiling by spectrophotometric methods of aqueous methanolic extracts of Helichrysum stoechas subsp. rupestre and Phagnalon saxatile subsp. saxatile.
abstract: OBJECTIVE
  The aqueous methanolic extracts of two plants from Algeria, Helichrysum stoechas subsp. rupestre and Phagnalon saxatile subsp. saxatile, were investigated for their antioxidant activity.
  
  
  METHODS
  Total phenolics, flavonoids, and tannins were determined by spectrophotometric techniques. In vitro antioxidant and radical scavenging profiling was determined by spectrophotometric methods, through: Total antioxidant capacity, and radical scavenging effects by the DPPH and ABTS methods, reducing and chelating power, and blanching inhibition of the β-carotene.
  
  
  RESULTS
  All of the extracts showed interesting antioxidant and radical scavenging activity. The highest contents in phenolics, tannins, and the highest total antioxidant capacity as gallic acid equivalents of 97.5 ± 0.33 mg GAE/g DW was obtained for the flowers of H. stoechas subsp. rupestre extract in the phosphomolybdenum assay. An extract of the leafy stems of P. saxatile subsp. saxatile revealed the highest content of flavonoids, and the highest antioxidant activity by the radical scavenging and β-carotene assays when compared with standards. The best activity was by the scavenging radical DPPH with an IC50 value of 5.65 ± 0.10 μg·mL(-1).
  
  
  CONCLUSIONS
  The studied medicinal plants could provide scientific evidence for some traditional uses in the treatment of diseases related to the production of reactive oxygen species (ROS) and oxidative stress.
authors: Laboratory of Natural Products, Department of Biology, Faculty of Sciences, Abou Bekr Belkaïd University, B. P 119, Tlemcen, 13000, Algeria. Electronic address: biofar23@yahoo.fr.
authors: Farah
authors: F
authors: Haddouchi
authors: Laboratory of Natural Products, Department of Biology, Faculty of Sciences, Abou Bekr Belkaïd University, B. P 119, Tlemcen, 13000, Algeria.
authors: Tarik Mohammed
authors: TM
authors: Chaouche
authors: Laboratory of Plant Adaptation to Abiotic Stresses, Biotechnologic Center in Borj-Cedria Technopol (CBBC), B. P 901, 2050 Hammam-Lif, Tunisia.
authors: Riadh
authors: R
authors: Ksouri
authors: Laboratory of Plant Adaptation to Abiotic Stresses, Biotechnologic Center in Borj-Cedria Technopol (CBBC), B. P 901, 2050 Hammam-Lif, Tunisia.
authors: Faten
authors: F
authors: Medini
authors: Department of Biotechnology, Faculty of Natural Science and Life, Abdelel Hamid Ibn Badiss University, Mostaganem, 27000, Algeria.
authors: Fatima Zohra
authors: FZ
authors: Sekkal
authors: Laboratory of Natural Products, Department of Biology, Faculty of Sciences, Abou Bekr Belkaïd University, B. P 119, Tlemcen, 13000, Algeria.
authors: Abdelhafid
authors: A
authors: Benmansour
mesh_terms: D000462:Algeria; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D052160:Benzothiazoles / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D036562:Helichrysum / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018514:Plant Structures / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods; D013451:Sulfonic Acids / Q000378:metabolism; D013634:Tannins / Q000032:analysis / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; D013634:Tannins; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activity; Helichrysum stoechas subsp. rupestre; Phagnalon saxatile subsp. saxatile; Phenolic compounds