title: Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage.
abstract: Chronic enteritis can produce an excess of reactive oxygen species resulting in cellular damage. Stanniocalcin-1(STC-1) reportedly possesses anti-oxidative activity, the aim of this study was to define more clearly the direct contribution of STC-1 to anti-oxidative stress in cattle. In this study, primary intestinal epithelial cells (IECs) were exposed to hydrogen peroxide (H2O2) for different time intervals to mimic chronic enteritis-induced cellular damage. Prior to treatment with 200 µM H2O2, the cells were transfected with a recombinant plasmid for 48 h to over-express STC-1. Acridine orange/ ethidium bromide (AO/EB) double staining and trypan blue exclusion assays were then performed to measure cell viability and apoptosis of the cells, respectively. The expression of STC-1 and apoptosis-related proteins in the cells was monitored by real-time PCR and Western blotting. The results indicated that both STC-1 mRNA and protein expression levels positively correlated with the duration of H2O2 treatment. H2O2 damaged the bovine IECs in a time-dependent manner, and this effect was attenuated by STC-1 over-expression. Furthermore, over- expression of STC-1 up-regulated Bcl-2 protein expression and slightly down-regulated caspase-3 production in the damaged cells. Findings from this study suggested that STC-1 plays a protective role in intestinal cells through an antioxidant mechanism.
authors: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors: Li-ming
authors: LM
authors: Wu
authors: Rui
authors: R
authors: Guo
authors: Lin
authors: L
authors: Hui
authors: Yong-gang
authors: YG
authors: Ye
authors: Jing-mei
authors: JM
authors: Xiang
authors: Chun-yun
authors: CY
authors: Wan
authors: Miao
authors: M
authors: Zou
authors: Rui
authors: R
authors: Ma
authors: Xiao-zhuan
authors: XZ
authors: Sun
authors: Shi-jin
authors: SJ
authors: Yang
authors: Ding-zong
authors: DZ
authors: Guo
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western / Q000662:veterinary; D053148:Caspase 3 / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism; D004751:Enteritis / Q000209:etiology / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D004847:Epithelial Cells / Q000378:metabolism; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D006023:Glycoproteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; C051795:teleocalcin; D006861:Hydrogen Peroxide; C505410:Casp3 protein, rat; D053148:Caspase 3
keywords: Bcl-2; chronic enteritis; oxidative damage; stanniocalcin-1