title: Antiproliferative evaluation of isofuranodiene on breast and prostate cancer cell lines.
abstract: The anticancer activity of isofuranodiene, extracted from Smyrnium olusatrum, was evaluated in human breast adenocarcinomas MDA-MB 231 and BT 474, and Caucasian prostate adenocarcinoma PC 3 cell lines by MTS assay. MTS assay showed a dose-dependent growth inhibition in the tumor cell lines after isofuranodiene treatment. The best antiproliferative activity of the isofuranodiene was found on PC 3 cells with an IC50 value of 29 μM, which was slightly less than the inhibition against the two breast adenocarcinoma cell lines with IC50 values of 59 and 55 μM on MDA-MB 231 and BT 474, respectively. Hoechst 33258 assay was performed in order to study the growth inhibition mechanism in prostate cancer cell line; the results indicate that isofuranodiene induces apoptosis. Overall, the understudy compound has a good anticancer activity especially towards the PC 3. On the contrary, it is less active on Chinese hamster ovary cells (CHO) and human embryonic kidney (HEK 293) appearing as a good candidate as a potential natural anticancer drug with low side effects.
authors: School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, Italy.
authors: Michela
authors: 0000-0002-8383-0813
authors: M
authors: Buccioni
authors: School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, Italy.
authors: Diego
authors: 0000-0002-6616-6885
authors: D
authors: Dal Ben
authors: School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, Italy.
authors: Catia
authors: C
authors: Lambertucci
authors: School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, Italy.
authors: Filippo
authors: F
authors: Maggi
authors: School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, Italy.
authors: Fabrizio
authors: F
authors: Papa
authors: School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, Italy.
authors: Ajiroghene
authors: 0000-0003-1852-6716
authors: A
authors: Thomas
authors: School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, Italy.
authors: Claudia
authors: C
authors: Santinelli
authors: School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, Italy.
authors: Gabriella
authors: 0000-0002-9856-9873
authors: G
authors: Marucci
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D005663:Furans / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D005663:Furans; C017897:isofuranodiene