title: Oxidative stress activates the transcription factors FoxO 1a and FoxO 3a in the hippocampus of rats exposed to low doses of ozone.
abstract: The exposure to low doses of ozone induces an oxidative stress state, which is involved in neurodegenerative diseases. Forkhead box O (FoxO) family of transcription factors are activated by oxidative signals and regulate cell proliferation and resistance to oxidative stress. Our aim was to study the effect of chronic exposure to ozone on the activation of FoxO 1a and FoxO 3a in the hippocampus of rats. Male Wistar rats were divided into six groups and exposed to 0.25 ppm of ozone for 0, 7, 15, 30, 60, and 90 days. After treatment, the groups were processed for western blotting and immunohistochemistry against FoxO 3a, Mn SOD, cyclin D2, FoxO 1a, and active caspase 3. We found that exposure to ozone increased the activation of FoxO 3a at 30 and 60 days and expression of Mn SOD at all treatment times. Additionally, increases in cyclin D2 from 7 to 90 days; FoxO 1a at 15, 30, and 60 days; and activate caspase 3 from 30 to 60 days of exposure were noted. The results indicate that ozone alters regulatory pathways related to both the antioxidant system and the cell cycle, inducing neuronal reentry into the cell cycle and apoptotic death.
authors: Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Apartado Postal No. 70250, 04510 Delegación Coyoacán, DF, Mexico.
authors: Nancy P
authors: NP
authors: Gómez-Crisóstomo
authors: Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Apartado Postal No. 70250, 04510 Delegación Coyoacán, DF, Mexico.
authors: Erika
authors: E
authors: Rodríguez Martínez
authors: Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Apartado Postal No. 70250, 04510 Delegación Coyoacán, DF, Mexico.
authors: Selva
authors: 0000-0002-5204-1416
authors: S
authors: Rivas-Arancibia
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D056742:Cyclin D2 / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins; D018384:Oxidative Stress / Q000187:drug effects*; D010126:Ozone / Q000008:administration & dosage / Q000494:pharmacology*; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D000071161:Forkhead Box Protein O1
chemical_list: D053148:Caspase 3; D056742:Cyclin D2; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D009419:Nerve Tissue Proteins; D010126:Ozone; D013482:Superoxide Dismutase; C499515:FOXO3 protein, rat; C074639:Foxo1 protein, rat; D000071161:Forkhead Box Protein O1