title: [Biomimetic mineralization of a single-layer reconstituted type I collagen model induced by sodium tripolyphosphate and polyacrylic acid].
abstract: OBJECTIVE
  To investigate the functions of sodium tripolyphosphate (STTP) and polyacrylic acid (PAA) in the process of collagen biomimetic mineralization. This would allow future applications to other collagen matrices such as bone collagen or 3-D collagen scaffolds.
  
  
  METHODS
  Glass cover slips and transmission electron microscopy (TEM) grids were coated with reconstituted typeIcollagen fibrils. Mineralization of the reconstituted collagens was demonstrated with scanning electron microscopy (SEM) and TEM using a Portland cement-containing resin composite and a phosphate-containing fluid in the presence of PAA and STTP. The rest were immersed in a biomimetic remineralization medium without PAA and/or STTP (control).
  
  
  RESULTS
  In the presence of PAA and STTP in the mineralization medium, intrafibrillar mineralization based on the non-classical crystallisation pathway could be identified. Mineral phases were evident within the collagen fibrils as early as 12 h after the initially-formed amorphous calcium phosphate nanoprecursors were transformed into apatite nanocrystals. Collagens at 72 h were heavily mineralized with periodically arranged intrafibrillar apatite platelets. Conversely, only large mineral spheres with no preferred association with collagen fibrils were observed in the absence of biomimetic analogues in the medium (control).
  
  
  CONCLUSIONS
  Intrafibrillar apatite deposition can be achieved via biomimetic mineralization system containing PAA and STTP when amorphous calcium phosphate precursor is stabilized.
authors: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
authors: Lisha
authors: L
authors: Gu
authors: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
authors: Sui
authors: S
authors: Mai
authors: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
authors: Yipin
authors: Y
authors: Qi
authors: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
authors: Qi
authors: Q
authors: Huang
authors: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China. Email: lingjq@mail.sysu.edu.cn.
authors: Junqi
authors: J
authors: Ling
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D001031:Apatites; D032701:Biomimetics; D001842:Bone and Bones; D002130:Calcium Phosphates; D003094:Collagen; D024042:Collagen Type I / Q000737:chemistry*; D003188:Composite Resins; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008903:Minerals; D010710:Phosphates; D011122:Polyphosphates / Q000737:chemistry*; D054457:Tissue Scaffolds
chemical_list: D000180:Acrylic Resins; D001031:Apatites; D002130:Calcium Phosphates; D024042:Collagen Type I; D003188:Composite Resins; D008903:Minerals; D010710:Phosphates; D011122:Polyphosphates; C519480:amorphous calcium phosphate; C006903:carbopol 940; D003094:Collagen; C005692:triphosphoric acid