title: Molecular characterisation of an endornavirus infecting the phytopathogen Sclerotinia sclerotiorum.
abstract: The complete sequence and genome organisation of an endornavirus from the phytopathogenic fungus Sclerotinia sclerotiorum isolate 11691 was described and the name Sclerotinia sclerotiorum endornavirus 1 (SsEV1/11691) proposed. The genome is 10,513 nucleotides (nts) long with a single open reading frame (ORF) that codes for a single polyprotein of 3459 amino acid (aa) residues. The polyprotein contains cysteine-rich region (CRR), viral methyltransferase (MTR), putative DEXDc, viral helicase (Hel), phytoreo_S7 (S7) and RNA-dependent RNA polymerase (RdRp) domains. The polyprotein and the conserved domains are phylogenetically related to endornaviruses. However, the coding strand of SsEV1/11691 does not contain a site-specific nick characteristic of most previously described endornaviruses. The elimination of SsEV1/11691 did not result in any significant changes in the host phenotype and virulence.
authors: School of Biological Sciences, The University of Auckland, PO Box 92019, Auckland 1010, New Zealand. Electronic address: mkha201@aucklanduni.ac.nz.
authors: Mahmoud E
authors: ME
authors: Khalifa
authors: School of Biological Sciences, The University of Auckland, PO Box 92019, Auckland 1010, New Zealand.
authors: Michael N
authors: MN
authors: Pearson
mesh_terms: D001203:Ascomycota / Q000821:virology*; D016000:Cluster Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D020815:Polyproteins / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins
keywords: Endornaviridae; Endornavirus; Mitovirus; Sclerotinia sclerotiorum; SsEV1; dsRNA