title: In vitro effects of Yunnan Baiyao on canine hemangiosarcoma cell lines.
abstract: Yunnan Baiyao is a Chinese herbal medicine that has been utilized for its anti-inflammatory, haemostatic, wound healing and pain relieving properties in people. It has been utilized in the veterinary profession to control bleeding in dogs with hemangiosarcoma (HSA) and has been anecdotally reported to prolong survival times in dogs with this neoplasm. This study evaluated the in vitro activity of Yunnan Baiyao against three canine HSA cell lines after treatment with increasing concentrations of Yunnan Baiyao (50, 100, 200, 400, 600 and 800 µg mL(-1) ) at 24, 48 and 72 h. Mean half maximum inhibitory concentration (IC50 ) at 72 h for DEN, Fitz, SB was 369.9, 275.9 and 325.3 µg mL(-1) , respectively. Caspase-3/7 activity increased in correlation with the IC50 in each cell line which was confirmed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, APO-BRDU Kit; BD Biosciences, San Jose, CA, USA) assay. VEGF in cell supernatant was also quantified. Overall, the study found that Yunnan Baiyao causes dose and time dependent HSA cell death through initiation of caspase-mediated apoptosis, which supports future studies involving Yunnan Baiyao.
authors: Department of Clinical Sciences, University of Florida, Gainesville, FL, USA.
authors: K A
authors: http://orcid.org/0000-0002-8590-6906
authors: KA
authors: Wirth
authors: Department of Clinical Sciences, University of Florida, Gainesville, FL, USA.
authors: K
authors: K
authors: Kow
authors: Department of Clinical Sciences, University of Florida, Gainesville, FL, USA.
authors: M E
authors: ME
authors: Salute
authors: Department of Clinical Sciences, University of Florida, Gainesville, FL, USA.
authors: N J
authors: NJ
authors: Bacon
authors: Department of Clinical Sciences, University of Florida, Gainesville, FL, USA.
authors: R J
authors: RJ
authors: Milner
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006394:Hemangiosarcoma / Q000188:drug therapy / Q000662:veterinary*; D020287:In Situ Nick-End Labeling; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D042461:Vascular Endothelial Growth Factor A; C530960:yunnan baiyao
keywords: Chinese herbal medicine; Yunnan Baiyao; canine hemangiosarcoma