title: Alkaline and alkaline peroxide pretreatments at mild temperature to enhance enzymatic hydrolysis of rice hulls and straw.
abstract: The current study explores alkaline and alkaline peroxide pretreatments in order to achieve a method to improve saccharification of agricultural residues for ethanol production. The effects of reagent concentration and pretreatment time at 30°C and atmospheric pressure on biomass dissolution after the pretreatment and enzymatic hydrolysis of the pretreated biomass were investigated. In fact, although all pretreatments tested improved enzymatic hydrolysis of native residues, the best results were not achieved for the highest biomass loss. The maximum conversions to reducing sugars in the hydrolysis stage of 77.5% and 92.6% were obtained for rice hulls and straw pretreated by alkaline peroxide (4%, 24h) and alkaline (1%, 48 h) methods, respectively. For both pretreated residues, the reduction to more than half the recommended enzyme loading allowed obtaining more than 94% the reducing sugars attained with the recommended dose.
authors: Departamento de Ingeniería Química, Instituto Superior Politécnico José Antonio Echeverría, Cujae, Ave. 114 No. 11901, Marianao 19390, Cuba. Electronic address: ecabrera@quimica.cujae.edu.cu.
authors: Emir
authors: E
authors: Cabrera
authors: Departamento de Ingeniería Química y Tecnología de Alimentos, Universidad de Cádiz, Campus Río San Pedro, s/n, Puerto Real 11510, Cádiz, Spain.
authors: María J
authors: MJ
authors: Muñoz
authors: Departamento de Ingeniería Química y Tecnología de Alimentos, Universidad de Cádiz, Campus Río San Pedro, s/n, Puerto Real 11510, Cádiz, Spain.
authors: Ricardo
authors: R
authors: Martín
authors: Departamento de Ingeniería Química y Tecnología de Alimentos, Universidad de Cádiz, Campus Río San Pedro, s/n, Puerto Real 11510, Cádiz, Spain.
authors: Ildefonso
authors: I
authors: Caro
authors: Departamento de Ingeniería Química, Instituto Superior Politécnico José Antonio Echeverría, Cujae, Ave. 114 No. 11901, Marianao 19390, Cuba.
authors: Caridad
authors: C
authors: Curbelo
authors: Departamento de Ingeniería Química y Tecnología de Alimentos, Universidad de Cádiz, Campus Río San Pedro, s/n, Puerto Real 11510, Cádiz, Spain.
authors: Ana B
authors: AB
authors: Díaz
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D002241:Carbohydrates / Q000032:analysis; D002480:Cellulase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006868:Hydrolysis / Q000187:drug effects; D012275:Oryza / Q000187:drug effects*; D012972:Sodium Hydroxide / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014866:Waste Products
chemical_list: D000468:Alkalies; D002241:Carbohydrates; D014866:Waste Products; D012972:Sodium Hydroxide; D006861:Hydrogen Peroxide; D002480:Cellulase
keywords: Alkaline peroxide pretreatment; Bioethanol; Enzymatic hydrolysis; Lignocellulosic residue; Sodium hydroxide pretreatment