title: miR-1 and miR-133b are differentially expressed in patients with recurrent prostate cancer.
abstract: Prostate cancer (PCa) is currently the most frequently diagnosed malignancy in the western countries. It is more prevalent in older men with 75% of the incident cases above 65 years old. After radical prostatectomy, approximately 30% of men develop clinical recurrence with elevated serum prostate-specific antigen levels. Therefore, it is important to unravel the molecular mechanisms underlying PCa progression to develop novel diagnostic/therapeutic approaches. In this study, it is aimed to compare the microRNA (miRNA) profile of recurrent and non-recurrent prostate tumor tissues to explore the possible involvement of miRNAs in PCa progression. Total RNA from 41 recurrent and 41 non-recurrent PCa tissue samples were used to investigate the miRNA signature in PCa specimens. First of all, 20 recurrent and 20 non-recurrent PCa samples were profiled using miRNA microarray chips. Of the differentially expressed miRNAs, miR-1, miR-133b and miR-145* were selected for further validation with qRT-PCR in a different set of 21 recurrent and 21 non-recurrent PCa samples. Data were statistically analyzed using two-sided Student's t-test, Pearson Correlation test, Receiver operating characteristic analysis. Our results demonstrated that miR-1 and mir-133b have been significantly downregulated in recurrent PCa specimens in comparison to non-recurrent PCa samples and have sufficient power to distinguish recurrent specimens from non-recurrent ones on their own. Here, we report that the relative expression of miR-1 and mir-133b have been significantly reduced in recurrent PCa specimens in comparison to non-recurrent PCa samples, which can serve as novel biomarkers for prediction of PCa progression.
authors: Department of Medical Genetics, Istanbul University, Cerrahpasa Medical School, Istanbul, Turkey; Molecular Biology and Genetics Department, Erzurum Technical University, Erzurum, Turkey.
authors: Omer Faruk
authors: OF
authors: Karatas
authors: Department of Medical Genetics, Istanbul University, Cerrahpasa Medical School, Istanbul, Turkey; Biruni University, Istanbul, Turkey.
authors: Esra
authors: E
authors: Guzel
authors: Department of Medical Genetics, Istanbul University, Cerrahpasa Medical School, Istanbul, Turkey.
authors: Ilknur
authors: I
authors: Suer
authors: Department of Medical Pathology, Yeditepe University, Istanbul, Turkey.
authors: Isin D
authors: ID
authors: Ekici
authors: Departmentof Urology, Goztepe Education and Research Hospital, Goztepe, Istanbul, Turkey.
authors: Turhan
authors: T
authors: Caskurlu
authors: Division of Biostatistics, Baylor College of Medicine, Houston, Texas, United States of America.
authors: Chad J
authors: CJ
authors: Creighton
authors: Department of Pathology & Immunology, Baylor College of Medicine, and Michael E. DeBakey VAMC, Houston, Texas, United States of America.
authors: Michael
authors: M
authors: Ittmann
authors: Department of Medical Genetics, Istanbul University, Cerrahpasa Medical School, Istanbul, Turkey; Biruni University, Istanbul, Turkey; Department of Pathology & Immunology, Baylor College of Medicine, and Michael E. DeBakey VAMC, Houston, Texas, United States of America.
authors: Mustafa
authors: M
authors: Ozen
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D015536:Down-Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D012008:Recurrence
chemical_list: D014408:Biomarkers, Tumor; C544547:MIRN1 microRNA, human; C548234:MIRN133 microRNA, human; D035683:MicroRNAs