title: Identification and transcript analysis of two glutamate decarboxylase genes, CsGAD1 and CsGAD2, reveal the strong relationship between CsGAD1 and citrate utilization in citrus fruit.
abstract: Glutamate decarboxylase (GAD, EC 4.1.1.15) has been suggested to be a key, regulatory point in the biosynthesis of γ-aminobutyrate (GABA) and in the utilization of citric acid through GABA shunt pathway. In this study we discovered two GAD genes, named as CsGAD1 and CsGAD2, in citrus genome database and then successfully cloned. Both CsGAD1 and CsGAD2 have a putative pyridoxal 5-phosphate binding domain in the middle region and a putative calmodulin-binding domain at the carboxyl terminus. Gene structure analysis showed that much difference exists in the size of exons and introns or in cis-regulatory elements in promoter region between the two GAD genes. Gene expression indicated that CsGAD1 transcript was predominantly expressed in flower and CsGAD2 transcript was predominantly expressed in fruit juice sacs; in the ripening fruit, CsGAD1 transcript level was at least 2-time higher than CsGAD2 transcript level. Moreover, CsGAD1 transcript level was increased significantly along with the increase of GAD activity and accompanied by a significant decrease of titratable acid (TA), suggesting that it is CsGAD1 rather than CsGAD2 plays a role in the citric acid utilization during fruit ripening. In addition, injection of abscisic acid and foliar spray of K2SO4 significantly increased the TA content of Satsuma mandarin, and significantly decreased GAD activity as well as CsGAD1 transcript, further suggesting the important role of CsGAD1 in the citrate utilization of citrus fruit.
authors: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors: Xiao
authors: X
authors: Liu
authors: Xiao-Mei
authors: XM
authors: Hu
authors: Long-Fei
authors: LF
authors: Jin
authors: Cai-Yun
authors: CY
authors: Shi
authors: Yong-Zhong
authors: YZ
authors: Liu
authors: Shu-Ang
authors: SA
authors: Peng
mesh_terms: D000595:Amino Acid Sequence; D019343:Citric Acid / Q000378:metabolism; D002957:Citrus / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005968:Glutamate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D019343:Citric Acid; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase