title: Synthesis of functionalized poly(ester carbonate) with laminin-derived peptide for promoting neurite outgrowth of PC12 cells.
abstract: Maleimide-functionalized poly(ester carbonate)s are synthesized by ring-opening copolymerization of furan-maleimide functionalized trimethylene carbonate (FMTMC) with L-lactide and a subsequent retro Diels-Alder reaction. The maleimide groups on poly(ester carbonate)s are amenable to Michael addition with thiol-containing molecules such as 3-mercapto-1-propanol, 2-aminoethanethiol hydrochloride, and mercaptoacetic acid under mild conditions, enabling the formation of biodegradable materials with various functional groups (e.g., hydroxyl, amine, and carboxyl). In particular, the maleimide-functionalized poly(ester carbonate) is clicked with a laminin-derived peptide CQAASIKVAV. In vitro culture of PC12 cells shows that the maleimide-functionalized polymers, especially the CQAASIKVAV-grafted one, could support cell proliferation and neurite outgrowth. The maleimide-functionalized poly(ester carbonate)s provide a versatile platform for diverse functionalization and have comprehensive potential in biomedical engineering.
authors: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, PR China.
authors: Dongming
authors: D
authors: Xing
authors: Lie
authors: L
authors: Ma
authors: Changyou
authors: C
authors: Gao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D057930:Click Chemistry; D061565:Cycloaddition Reaction; D004146:Dioxanes / Q000737:chemistry*; D005663:Furans / Q000737:chemistry; D006133:Growth Substances / Q000138:chemical synthesis* / Q000494:pharmacology; D007797:Laminin / Q000737:chemistry*; D008301:Maleimides / Q000737:chemistry; D008969:Molecular Sequence Data; D016501:Neurites / Q000187:drug effects* / Q000648:ultrastructure; D016716:PC12 Cells; D011091:Polyesters / Q000138:chemical synthesis* / Q000494:pharmacology; D051381:Rats
chemical_list: D004146:Dioxanes; D005663:Furans; D006133:Growth Substances; D007797:Laminin; D008301:Maleimides; D011091:Polyesters; C043592:maleimide; C443643:trimethylene carbonate; C091880:dilactide
keywords: PC12 cells; biomaterials; maleimide; neurite outgrowth; poly(ester carbonate)s