title: Molecular characterization of Indian sheeppox and goatpox viruses based on RPO30 and GPCR genes.
abstract: Sheeppox and goatpox are economically important diseases of small ruminants caused by sheeppox virus (SPPV) and goatpox virus (GTPV), respectively. Although SPPV and GTPV have host preference, some strains may infect both sheep and goats. As capripox viruses (SPPV, GTPV and LSDV) are antigenically related but genetically distinct, their differentiation requires analysis at molecular level. In the present study, RPO30 and GPCR genes of eight Indian SPPV and GTPV isolates were PCR amplified, cloned and sequences are genetically and phylogenetically analyzed. The RPO30 gene of SPPV and GTPV had lineage-specific signatures, and deletion of 21-nucleotide exclusively present in SPPV. Similarly, GPCR gene also had lineage-specific signatures for SPPV and GTPV. Phylogenetic analysis of capripox viruses based on RPO30 and GPCR genes revealed three distinct lineage-specific clusters as per their host origin. Our study supports that both RPO30 and GPCR genes could be used for differentiation of SPPV and GTPV as well as for molecular epidemiological studies. The study also highlights the distinct lineage specificities of the Indian SPPV and GTPV isolates including vaccine strains.
authors: Division of Virology, Indian Veterinary Research Institute, Mukteswar, 263 138, Uttarakhand, India.
authors: Ramasamy
authors: R
authors: Santhamani
authors: Revanaiah
authors: R
authors: Yogisharadhya
authors: Gnanavel
authors: G
authors: Venkatesan
authors: Sathish Bhadravati
authors: SB
authors: Shivachandra
authors: Awadh Bihari
authors: AB
authors: Pandey
authors: Muthannan Andavar
authors: MA
authors: Ramakrishnan
mesh_terms: D000818:Animals; D018151:Capripoxvirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D005814:Genes, Viral; D014644:Genetic Variation; D005838:Genotype; D006041:Goats; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012756:Sheep
chemical_list: D004279:DNA, Viral