title: Transgenic increase of Wnt7b in neural progenitor cells decreases expression of T-domain transcription factors and impairs neuronal differentiation.
abstract: Wnt/beta-catenin signaling plays an important role in neural development, instructing both progenitor cell division and differentiation. During early corticogenesis, Wnt7b is expressed in a restricted expression pattern in the ventricular zone progenitor cells. However, its influence on progenitor cell behavior has not been fully studied. We report that transgenic overexpression of Wnt7b in neural progenitor cells impairs neuronal differentiation and the development of forebrain structures at embryonic day 10.5 (E10.5). This was accompanied by a decreased expression of T-domain transcription factors Tbr1 and Tbr2, in both progenitor cells and post-mitotic neurons. However, proliferation, apoptosis and the overall proportion of pax6(+) neural progenitor cells were similar to wild-type litter mates. These results suggest that Wnt signaling may affect early neural progenitor differentiation by regulating the expression of pro-neural transcription factors.
authors: Neonatal Research Unit, Astrid Lindgren Children׳s Hospital, Q2:07, Karolinska Institutet, SE-171 76 Stockholm, Sweden. Electronic address: panos.papachristou@ki.se.
authors: Panagiotis
authors: P
authors: Papachristou
authors: Neonatal Research Unit, Astrid Lindgren Children׳s Hospital, Q2:07, Karolinska Institutet, SE-171 76 Stockholm, Sweden.
authors: Cecilia
authors: C
authors: Dyberg
authors: Neonatal Research Unit, Astrid Lindgren Children׳s Hospital, Q2:07, Karolinska Institutet, SE-171 76 Stockholm, Sweden.
authors: Maria
authors: M
authors: Lindqvist
authors: Neonatal Research Unit, Astrid Lindgren Children׳s Hospital, Q2:07, Karolinska Institutet, SE-171 76 Stockholm, Sweden.
authors: Zachi
authors: Z
authors: Horn
authors: Neonatal Research Unit, Astrid Lindgren Children׳s Hospital, Q2:07, Karolinska Institutet, SE-171 76 Stockholm, Sweden.
authors: Thomas
authors: T
authors: Ringstedt
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism; D003599:Cytoskeleton / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D015536:Down-Regulation; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005813:Genes, Synthetic; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008845:Microinjections; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000502:physiology; D064231:Nestin / Q000235:genetics; D058953:Neural Stem Cells / Q000378:metabolism; D054259:Neural Tube / Q000648:ultrastructure; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D016548:Prosencephalon / Q000196:embryology / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D020825:T-Box Domain Proteins / Q000096:biosynthesis* / Q000235:genetics; D014404:Tubulin / Q000032:analysis; D051153:Wnt Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015053:Zygote
chemical_list: D004268:DNA-Binding Proteins; C491198:Eomes protein, mouse; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D011518:Proto-Oncogene Proteins; D020825:T-Box Domain Proteins; C492271:Tbr1 protein, mouse; D014404:Tubulin; D051153:Wnt Proteins; C095937:Wnt7b protein, mouse
keywords: Early forebrain development; Nestin; Neural progenitor cell; Neuronal differentiation; T-domain transcription factor; Wnt signaling