title: Transmission line model analysis of transcription factors binding to oligoduplexes - differentiation of the effect of single nucleotide modifications.
abstract: Advanced impedance spectroscopy analysis based on the transmission line model (TLM) is explored as a novel QCM acoustic biosensing platform for the detection of the single point mutation effect on the binding of the transcription factors (TFs) to immobilized DNA oligoduplexes and the characterization of the protein-DNA mechanical properties.
authors: IBB-Institute for Biotechnology and Bioengineering, Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, 8005-139 Faro, Portugal. gferrei@ualg.pt.
authors: Rogério M M
authors: RM
authors: Rodrigues
authors: Jorge
authors: J
authors: de-Carvalho
authors: Silvia F
authors: SF
authors: Henriques
authors: Nuno P
authors: NP
authors: Mira
authors: Isabel
authors: I
authors: Sá-Correia
authors: Guilherme N M
authors: GN
authors: Ferreira
mesh_terms: D058266:Dielectric Spectroscopy / Q000379:methods*; D061925:Immobilized Nucleic Acids / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D017354:Point Mutation; D011485:Protein Binding; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: C439059:Haa1 protein, S cerevisiae; D061925:Immobilized Nucleic Acids; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors