title: Estimation of the hydrogen concentration in rat tissue using an airtight tube following the administration of hydrogen via various routes.
abstract: Hydrogen exerts beneficial effects in disease animal models of ischemia-reperfusion injury as well as inflammatory and neurological disease. Additionally, molecular hydrogen is useful for various novel medical and therapeutic applications in the clinical setting. In the present study, the hydrogen concentration in rat blood and tissue was estimated. Wistar rats were orally administered hydrogen super-rich water (HSRW), intraperitoneal and intravenous administration of hydrogen super-rich saline (HSRS), and inhalation of hydrogen gas. A new method for determining the hydrogen concentration was then applied using high-quality sensor gas chromatography, after which the specimen was prepared via tissue homogenization in airtight tubes. This method allowed for the sensitive and stable determination of the hydrogen concentration. The hydrogen concentration reached a peak at 5 minutes after oral and intraperitoneal administration, compared to 1 minute after intravenous administration. Following inhalation of hydrogen gas, the hydrogen concentration was found to be significantly increased at 30 minutes and maintained the same level thereafter. These results demonstrate that accurately determining the hydrogen concentration in rat blood and organ tissue is very useful and important for the application of various novel medical and therapeutic therapies using molecular hydrogen.
authors: 1] Division of Transplantation Immunology, National Research Institute for Child Health and Development [2] Preclinical Medical Institute, Gannan Medical University, Jiangxi, China [3].
authors: Chi
authors: C
authors: Liu
authors: 1] Division of Transplantation Immunology, National Research Institute for Child Health and Development [2] MiZ Co., Ltd., Kanagawa; Japan [3].
authors: Ryosuke
authors: R
authors: Kurokawa
authors: 1] Division of Transplantation Immunology, National Research Institute for Child Health and Development [2] AIDS Research Center, National Institute of Infectious Diseases.
authors: Masayuki
authors: M
authors: Fujino
authors: MiZ Co., Ltd., Kanagawa; Japan.
authors: Shinichi
authors: S
authors: Hirano
authors: MiZ Co., Ltd., Kanagawa; Japan.
authors: Bunpei
authors: B
authors: Sato
authors: Division of Transplantation Immunology, National Research Institute for Child Health and Development.
authors: Xiao-Kang
authors: XK
authors: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001774:Blood Chemical Analysis; D006859:Hydrogen / Q000008:administration & dosage* / Q000032:analysis* / Q000493:pharmacokinetics; D007274:Injections, Intraperitoneal; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000494:pharmacology*; D014018:Tissue Distribution
chemical_list: D012965:Sodium Chloride; D006859:Hydrogen