title: (1)H high resolution magic-angle coil spinning (HR-MACS) μNMR metabolic profiling of whole Saccharomyces cervisiae cells: a demonstrative study.
abstract: The low sensitivity and thus need for large sample volume is one of the major drawbacks of Nuclear Magnetic Resonance (NMR) spectroscopy. This is especially problematic for performing rich metabolic profiling of scarce samples such as whole cells or living organisms. This study evaluates a (1)H HR-MAS approach for metabolic profiling of small volumes (250 nl) of whole cells. We have applied an emerging micro-NMR technology, high-resolution magic-angle coil spinning (HR-MACS), to study whole Saccharomyces cervisiae cells. We find that high-resolution high-sensitivity spectra can be obtained with only 19 million cells and, as a demonstration of the metabolic profiling potential, we perform two independent metabolomics studies identifying the significant metabolites associated with osmotic stress and aging.
authors: CEA Saclay, DSM, IRAMIS, UMR CEA/CNRS 3299 - NIMBE, Laboratoire Structure et Dynamique par Résonance Magnétique Gif-sur-Yvette, France.
authors: Alan
authors: A
authors: Wong
authors: CEA Saclay, DSM, IRAMIS, UMR CEA/CNRS 3299 - NIMBE, Laboratoire Structure et Dynamique par Résonance Magnétique Gif-sur-Yvette, France.
authors: Céline
authors: C
authors: Boutin
authors: Department of Chemistry, University of York Heslington, York, UK.
authors: Pedro M
authors: PM
authors: Aguiar
keywords: HR-MACS; Metabolic Profiling; Saccharomyces cervisiae; cell growth; micro-NMR; osmotic stress