title: Phytotoxic and genotoxic effects of silver nanoparticles exposure on germinating wheat seedlings.
abstract: We investigated the effects of 1 and 10 mg L(-1) AgNPs on germinating Triticum aestivum L. seedlings. The exposure to 10 mg L(-1) AgNPs adversely affected the seedling growth and induced morphological modifications in root tip cells. TEM analysis suggests that the observed effects were due primarily to the release of Ag ions from AgNPs. To gain an increased understanding of the molecular response to AgNP exposure, we analyzed the genomic and proteomic changes induced by AgNPs in wheat seedlings. At the DNA level, we applied the AFLP technique and we found that both treatments did not induce any significant DNA polymorphisms. 2DE profiling of roots and shoots treated with 10 mg L(-1) of AgNPs revealed an altered expression of several proteins mainly involved in primary metabolism and cell defense.
authors: Dipartimento Biotecnologie e Scienze della Vita, Università degli Studi dell' Insubria, Via J.H. Dunant 3, 21100 Varese, Italy. Electronic address: candida.vannini@uninsubria.it.
authors: Candida
authors: C
authors: Vannini
authors: Dipartimento Biotecnologie e Scienze della Vita, Università degli Studi dell' Insubria, Via J.H. Dunant 3, 21100 Varese, Italy.
authors: Guido
authors: G
authors: Domingo
authors: Dipartimento Bioscienze, Università degli Studi di Milano, Via G. Celoria 26, 20133 Milano, Italy.
authors: Elisabetta
authors: E
authors: Onelli
authors: Dipartimento Biotecnologie e Bioscienze, Università degli Studi di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors: Fabrizio
authors: F
authors: De Mattia
authors: Dipartimento Biotecnologie e Bioscienze, Università degli Studi di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors: Ilaria
authors: I
authors: Bruni
authors: Dipartimento Biotecnologie e Scienze della Vita, Università degli Studi dell' Insubria, Via J.H. Dunant 3, 21100 Varese, Italy.
authors: Milena
authors: M
authors: Marsoni
authors: Dipartimento Biotecnologie e Scienze della Vita, Università degli Studi dell' Insubria, Via J.H. Dunant 3, 21100 Varese, Italy.
authors: Marcella
authors: M
authors: Bracale
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D004249:DNA Damage / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D018525:Germination / Q000187:drug effects; D013058:Mass Spectrometry; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000633:toxicity*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D040901:Proteomics; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D012834:Silver / Q000633:toxicity*; D013312:Stress, Physiological; D014908:Triticum / Q000187:drug effects* / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure
chemical_list: D012834:Silver
keywords: AFLP; Proteomics; Silver nanoparticles; TEM; Triticum aestivum