title: Study on the interaction between bovine serum albumin and 4'-azido-2'-deoxyfluoroarabinocytidine or analogs by spectroscopy and molecular modeling.
abstract: The binding of 4'-azido-2'-deoxyfluoroarabinocytidine (FNC) or analogs (cytidine and 5'-cytidylate monophosphate) to bovine serum albumin (BSA) was investigated by fluorescence, UV-vis absorption spectroscopy and molecular modeling. The three compounds quenched the intrinsic fluorescence of BSA and the results revealed the presence of static quenching mechanism. The positive ΔH and positive ΔS for the systems suggested that the hydrophobic forces stabilized the interaction between the compounds and protein. Results also showed that FNC was the weakest quencher.
authors: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou 450001, China. Electronic address: wangry@zzu.edu.cn.
authors: Ruiyong
authors: R
authors: Wang
authors: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou 450001, China.
authors: Xiaogai
authors: X
authors: Wang
authors: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou 450001, China.
authors: Zhigang
authors: Z
authors: Li
authors: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou 450001, China.
authors: Yuanzhe
authors: Y
authors: Xie
authors: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou 450001, China.
authors: Lingling
authors: L
authors: Yang
authors: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou 450001, China.
authors: Jie
authors: J
authors: Shi
authors: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou 450001, China. Electronic address: changjunbiao@zzu.edu.cn.
authors: Junbiao
authors: J
authors: Chang
mesh_terms: D000818:Animals; D002417:Cattle; D003562:Cytidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D004735:Energy Transfer; D007700:Kinetics; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: C000595802:4'-azido-2'-deoxyfluoroarabinocytidine; D003841:Deoxycytidine; D012710:Serum Albumin, Bovine; D003562:Cytidine
keywords: Analogs; Bovine serum albumin; FNC; Spectroscopy