title: IQPA: isothermal nucleic acid amplification-based immunoassay using DNAzyme as the reporter system.
abstract: Immunoassays are often coupled to peroxidase activity for antigen detection. Sensitivity and speed of detection has been increased by the advent of hybrid methods such as immuno-PCR (polymerase chain reaction). However, a more simplified immunoassay that retains both colorimetric peroxidase detection and effective DNA amplification in a setting closer to field application conditions has been nonexistent. Here we describe a method that successfully combines a competitive immunoassay with the new isothermal quadruplex-primed amplification (QPA) to generate excess quadruplex reporter molecules with intrinsic peroxidase DNAzyme activity.
authors: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang 11800, Malaysia.
authors: Qiuting
authors: Q
authors: Loh
authors: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang 11800, Malaysia.
authors: Noorsharmimi
authors: N
authors: Omar
authors: Department of Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Wildau 15745, Germany.
authors: Jörn
authors: J
authors: Glökler
authors: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang 11800, Malaysia. Electronic address: theamsoon@usm.my.
authors: Theam Soon
authors: TS
authors: Lim
mesh_terms: D004247:DNA / Q000032:analysis* / Q000378:metabolism; D021881:DNA, Catalytic / Q000378:metabolism*; D054856:G-Quadruplexes; D007118:Immunoassay / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques
chemical_list: D021881:DNA, Catalytic; D004247:DNA
keywords: Antibody–antigen detection; Biosensors; DNAzyme; Isothermal amplification