title: Interaction between Theileria orientalis 23-kDa piroplasm membrane protein and heparin.
abstract: The 23-kDa piroplasm membrane protein of Theileria orientalis (p23) is an immunogenic protein expressed during the intraerythrocytic stage of the parasite; its function, however, remains unclear. To evaluate the host factor or factors that interact with p23, we examined the binding of p23 to components of the host cell surface. Recombinant p23 protein of the Ikeda genotype failed to bind to bovine red blood cells or to peripheral blood mononuclear cells, but did bind to Madin-Darby Bovine Kidney (MDBK) cells. A glycoarray assay showed that recombinant p23 proteins from the three genotypes bound to heparin, indicating that p23 is a heparin-binding Theileria surface molecule. Further analysis of heparin-binding molecules is useful for understanding attachment and invasion of T. orientalis merozoites.
authors: Hitoshi
authors: H
authors: Takemae
authors: Tatsuki
authors: T
authors: Sugi
authors: Kyousuke
authors: K
authors: Kobayashi
authors: Fumi
authors: F
authors: Murakoshi
authors: Frances C
authors: FC
authors: Recuenco
authors: Akiko
authors: A
authors: Ishiwa
authors: Atsuko
authors: A
authors: Inomata
authors: Taisuke
authors: T
authors: Horimoto
authors: Naoaki
authors: N
authors: Yokoyama
authors: Kentaro
authors: K
authors: Kato
authors: Kentaro
authors: K
authors: Kato
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D005838:Genotype; D006493:Heparin / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016794:Theileria / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D009844:Oligosaccharides; D011994:Recombinant Proteins; D006493:Heparin