title: Microbial sequencing analyses suggest the presence of a fecal veneer on indoor climbing wall holds.
abstract: Artificial climbing walls represent a unique indoor environment in which humans interact closely with a variety of surface types. Climbing wall holds may mediate transmission of organisms between individuals, and yet there are no studies that identify microorganisms present on these surfaces. In the current study, the microorganisms found on climbing wall holds were characterized by analysis of amplified SSU rRNA gene sequences. In contrast to many other studies of built environments, the majority of microorganisms on holds were most closely related to microbes annotated as being recovered from environmental sources, such as soil, with human skin also representing an important source. Regional patterns were evident as rRNA gene sequences from the marine cyanobacterium Prochlorococcus were abundant in gyms found within 16 km of the ocean. Enterobacteriaceae were present on 100 % of holds surveyed, and the members detected are commonly associated with fecal matter.
authors: Department of Biology, Appalachian State University, Boone, NC, 28608, USA, brauersl@appstate.edu.
authors: S L
authors: SL
authors: Bräuer
authors: D
authors: D
authors: Vuono
authors: M J
authors: MJ
authors: Carmichael
authors: C
authors: C
authors: Pepe-Ranney
authors: A
authors: A
authors: Strom
authors: E
authors: E
authors: Rabinowitz
authors: D H
authors: DH
authors: Buckley
authors: S H
authors: SH
authors: Zinder
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D058448:Biota; D016000:Cluster Analysis; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013177:Sports
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S