title: Downregulation of uncoupling protein-2 by genipin exacerbates diabetes-induced kidney proximal tubular cells apoptosis.
abstract: Renal tubular epithelial cell injury is a major pathological event that contributes to the development of diabetic kidney disease (DKD). Uncoupling protein-2 (UCP2), a mitochondrial membrane protein, has been reported to participate in the regulation of reactive oxygen species (ROS) generation, which contributes to tubular cell apoptosis induced by hyperglycemia. In this study, we found that genipin, a UCP2 inhibitor, dramatically boosted oxidative stress, attenuated antioxidative capacity, and exacerbated cell apoptosis accompanied with caspase-3 activation in rat renal proximal tubular cells (NRK-52E) incubated with high glucose. The present study results suggest that manipulation of UCP2 could be important in the prevention of oxidative stress damage in renal tubular epithelial cells induced by hyperglycemia in vitro.
authors: Department of Nephrology, West China Hospital, Sichuan University , Chengdu , China and.
authors: Xiao-lei
authors: XL
authors: Chen
authors: Wan-Xin
authors: WX
authors: Tang
authors: Xiao-hong
authors: XH
authors: Tang
authors: Wei
authors: W
authors: Qin
authors: Meng
authors: M
authors: Gong
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology*; D002478:Cells, Cultured; D003928:Diabetic Nephropathies / Q000503:physiopathology*; D015536:Down-Regulation; D007473:Ion Channels / Q000502:physiology*; D039823:Iridoids / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000166:cytology*; D024101:Mitochondrial Proteins / Q000502:physiology*; D051381:Rats; D000071246:Uncoupling Protein 2
chemical_list: D007473:Ion Channels; D039823:Iridoids; D024101:Mitochondrial Proteins; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2; C007834:genipin
keywords: Diabetic kidney disease; genipin; oxidative stress; renal tubular cell; uncoupling protein-2