title: Tissue distribution and excretion study of neopanaxadiol in rats by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry.
abstract: Neopanaxadiol (NPD), a major ginsenoside in Panax ginseng C. A. Meyer (Araliaceae), was reported to have neuroprotective effect. In this study, a method of ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC/QTOF-MS) was developed and validated for quantitative analysis of NPD in tissues, urine and feces, using liquid-liquid extraction (LLE) to isolate NPD from different biological samples, and chromatographic separation was performed on an Agilent Zorbax Stable Bond C18 (2.1 × 50 mm, 1.8 µm) column with 0.1% formic acid in water and acetonitrile. All standard calibration curves were linear (all r(2) > 0.995) within the test range. After oral administration, NPD was extensively distributed to most of the tissues without long-term accumulation. The higher levels were observed in stomach and intestine, followed by kidney and liver. Approximately 64.56 ± 20.32% of administered dose in feces and 0.0233 ± 0.0356% in urine were found within 96 h, which indicated that the major elimination route was fecal excretion. This analytical method was applied to the study of NPD distribution and excretion in rats after oral intake for the first time. The results we found here are helpful for us to understand the pharmacological effects of NPD, as well as its toxicity.
authors: Department of Natural products Chemistry, College of Pharmacy, Jilin University, 1266 Fujin Road, Changchun, 130021, People's Republic of China.
authors: Cong
authors: C
authors: Geng
authors: Jian-yuan
authors: JY
authors: Yin
authors: Xiu-hua
authors: XH
authors: Yu
authors: Jing-yan
authors: JY
authors: Liu
authors: Yu-xia
authors: YX
authors: Yang
authors: De-ya
authors: DY
authors: Sun
authors: Qin
authors: Q
authors: Meng
authors: Zhong-lin
authors: ZL
authors: Wei
authors: Ji-hua
authors: JH
authors: Liu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces; D036145:Ginsenosides / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics*; D059625:Liquid-Liquid Extraction; D013058:Mass Spectrometry / Q000379:methods*; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D014018:Tissue Distribution
chemical_list: D036145:Ginsenosides; C000601507:dammar-20(22)-ene-3,12,25-triol
keywords: UPLC-QTOF/MS; distribution; excretion; ginsenoside; neopanaxadiol