title: Electrospray Quadrupole Travelling Wave Ion Mobility Time-of-Flight Mass Spectrometry for the Detection of Plasma Metabolome Changes Caused by Xanthohumol in Obese Zucker (fa/fa) Rats.
abstract: This study reports on the use of traveling wave ion mobility quadrupole time-of-flight (ToF) mass spectrometry for plasma metabolomics. Plasma metabolite profiles of obese Zucker fa/fa rats were obtained after the administration of different oral doses of Xanthohumol; a hop-derived dietary supplement. Liquid chromatography coupled data independent tandem mass spectrometry (LC-MSE) and LC-ion mobility spectrometry (IMS)-MSE acquisitions were conducted in both positive and negative modes using a Synapt G2 High Definition Mass Spectrometry (HDMS) instrument. This method provides identification of metabolite classes in rat plasma using parallel alternating low energy and high energy collision spectral acquisition modes. Data sets were analyzed using pattern recognition methods. Statistically significant (p < 0.05 and fold change (FC) threshold > 1.5) features were selected to identify the up-/down-regulated metabolite classes. Ion mobility data visualized using drift scope software provided a graphical read-out of differences in metabolite classes.
authors: Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA. samanthi.wickramasekara@oregonstate.edu.
authors: Samanthi I
authors: SI
authors: Wickramasekara
authors: Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA. zandkarf@onid.orst.edu.
authors: Fereshteh
authors: F
authors: Zandkarimi
authors: Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA. jeff.morre@oregonstate.edu.
authors: Jeff
authors: J
authors: Morré
authors: Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR 97331, USA. kirkwooj@onid.orst.edu.
authors: Jay
authors: J
authors: Kirkwood
authors: Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR 97331, USA. adrian.gombart@oregonstate.edu.
authors: LeeCole
authors: L
authors: Legette
authors: Department of Statistics, Oregon State University, Corvallis, OR 97331, USA. yuan.jiang@stat.oregonstate.edu.
authors: Yuan
authors: Y
authors: Jiang
authors: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA. adrian.gombart@oregonstate.edu.
authors: Adrian F
authors: AF
authors: Gombart
authors: Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR 97331, USA. adrian.gombart@oregonstate.edu.
authors: Jan F
authors: JF
authors: Stevens
authors: Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA. claudia.maier@oregonstate.edu.
authors: Claudia S
authors: CS
authors: Maier