title: CTL responses to DCs stimulated with leishmania antigens detected by DCs expressing Leishmania gp63.
abstract: BACKGROUND
  Leishmania is a pathogenic parasite which infects mononuclear cells in vertebrate hosts. Different strategies have been taken to develop immunity against Leishmania. DCs loaded with immunogenic antigen have resulted in different levels of Th1-type immune response and cytotoxic T lymphocytes (CTL) activity.
  
  
  OBJECTIVE
  To evaluate the potency of DCs primed with soluble Leishmania mexicana antigens (SLA) in developing CTL activity.
  
  
  METHODS
  DCs were loaded with SLA and injected to Balb/c mice. After two weeks the mice were sacrificed and their splenocytes were used as effector cells in a standard 4-hour cytotoxicity assay against DCs transfected with pcDNA3 containing L. mexicana gp63 gene.
  
  
  RESULTS
  Immunization of Balb/c mice with DCs loaded with SLA resulted in high levels of CTL activity against DCs transfected with pcDNA3 containing L. mexicana gp63 gene.
  
  
  CONCLUSIONS
  The results indicate a high potency for DCs primed with Leishmania antigens in inducing CTL activity, which can be used for developing an immunogenic vaccine against Leishmania.
authors: Department of Laboratory Sciences, School of Paraveterinary Sciences, Bu-Ali Sina University, Hamadan, Iran, e-mail: hrezvan@gmail.com.
authors: Hossein
authors: H
authors: Rezvan
authors: Ali
authors: A
authors: Khodadadi
authors: Selman A
authors: SA
authors: Ali
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression; D016130:Immunophenotyping; D007891:Leishmania / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology; D008666:Metalloendopeptidases / Q000235:genetics / Q000276:immunology*; D051379:Mice; D010641:Phenotype; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania