title: Coordinated expression of cyclin-dependent kinase-4 and its regulators in human oral tumors.
abstract: OBJECTIVE
  While aberrant expression of cyclin-dependent kinase-4 (CDK4) has been found in squamous cell carcinoma of the head and neck (SCCHN), the associations between CDK4 and its regulators, namely, cyclin D1, cyclin E, gankyrin, SEI1, and BMI1 in gene expression remain to be explored. Herein we investigated the mRNA profiles of these oncogenes and their interrelations in different oral lesion tissues.
  
  
  METHODS
  Thirty SCCHN specimens and patient-matched high at-risk mucosa (HARM) and 16 healthy control specimens were subjected to quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses.
  
  
  RESULTS
  The mRNA levels of CDK4, cyclin D1, gankyrin, SEI1, BMI1 were significantly elevated in both HARM and SCCHN (in comparison with control specimens), and statistically significant correlations were found among these markers in gene expression.
  
  
  CONCLUSIONS
  Up-regulation of CDK4 and its regulators takes place in oral cancer progression in a coordinate manner, and HARM and SCCHN share a similar molecular signature within the CDK4-pRB pathway.
authors: Department of Pharmacy, The Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, Columbus, OH, U.S.A Division of Pharmacy Practice and Administration, College of Pharmacy, The Ohio State University, Columbus, OH, U.S.A.
authors: Ming J
authors: MJ
authors: Poi
authors: Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH, U.S.A Comprehensive Cancer Center, The Ohio State University, Columbus, OH, U.S.A.
authors: Thomas J
authors: TJ
authors: Knobloch
authors: Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH, U.S.A.
authors: Marta T
authors: MT
authors: Sears
authors: Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH, U.S.A.
authors: Lana K
authors: LK
authors: Uhrig
authors: Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH, U.S.A College of Dentistry, The Ohio State University, Columbus, OH, U.S.A.
authors: Blake M
authors: BM
authors: Warner
authors: Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH, U.S.A Comprehensive Cancer Center, The Ohio State University, Columbus, OH, U.S.A Department of Otolaryngology-Head and Neck Surgery, The Ohio State University Wexner Medical Center, Columbus, OH, U.S.A.
authors: Christopher M
authors: CM
authors: Weghorst
authors: Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH, U.S.A Comprehensive Cancer Center, The Ohio State University, Columbus, OH, U.S.A li.225@osu.edu.
authors: Junan
authors: J
authors: Li
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D019927:Cyclin E / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D005260:Female; D006258:Head and Neck Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D048054:Mitogen-Activated Protein Kinase 7 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D025341:Principal Component Analysis; D046988:Proteasome Endopeptidase Complex / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077195:Squamous Cell Carcinoma of Head and Neck; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors; D015854:Up-Regulation
chemical_list: C530104:CCND1 protein, human; D019927:Cyclin E; D009687:Nuclear Proteins; C403591:PSMD10 protein, human; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C402464:SERTAD1 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D019938:Cyclin D1; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; C481581:MAPK7 protein, human; D048054:Mitogen-Activated Protein Kinase 7; D046988:Proteasome Endopeptidase Complex
keywords: Cyclin-dependent kinase 4; coordination; squamous cell carcinoma of the head and neck; transcription up-regulation