title: Effect of genetic factors on the association between coronary artery disease and PTPN22 polymorphism.
abstract: PTPN22 has been previously found associated with coronary artery disease (CAD). In the present note we have studied the effect of p53 codon 72, acid phosphatse locus 1 (ACP1) and adenosine deaminase (ADA) genetic polymorphism on the strength of association between PTPN22 and CAD. We have studied 133 non diabetic subjects with CAD, 122 non diabetic cardiovascular patients without CAD and 269 healthy blood donors. Informed written consent was obtained from all subjects and the study was approved by the Ethical Committee. A high significant association between PTPN22 and CAD is observed in carriers of *A allele of ACP1 with a higher proportion of *T allele carriers in non diabetic subjects with CAD as compared to controls and to non diabetic subjects with cardiovascular disease without CAD. A similar pattern is observed in carriers of *Pro allele of p53 codon 72 with a higher proportion of *T allele carriers in non diabetic subjects with CAD as compared to other groups. A highly significant association between PTPN22 and CAD is observed in carriers of ADA2 *2 allele with higher proportion of *T allele carriers in non diabetic subjects with CAD as compared to other group. There is a high significant correlation between the number of factors that contributes to increase the strength of association between PTPN22 *T and CAD and the proportion of *T carriers in CAD. ACP1, p53 codon 72 and ADA are involved in immune reaction and give an important additive contribution to the strength of association between PTPN22 and CAD. This study stresses the importance of the simultaneous analysis of multiple genes functionally related to a specific disease: the approach may give important hints to understand multifactorial disorders.
authors: Fulvia Gloria-Bottini, Patrizia Saccucci, Egidio Bottini, Department of Biomedicine and Prevention, School of Medicine, University of Rome Tor Vergata, 00133 Rome, Italy.
authors: Fulvia
authors: F
authors: Gloria-Bottini
authors: Fulvia Gloria-Bottini, Patrizia Saccucci, Egidio Bottini, Department of Biomedicine and Prevention, School of Medicine, University of Rome Tor Vergata, 00133 Rome, Italy.
authors: Patrizia
authors: P
authors: Saccucci
authors: Fulvia Gloria-Bottini, Patrizia Saccucci, Egidio Bottini, Department of Biomedicine and Prevention, School of Medicine, University of Rome Tor Vergata, 00133 Rome, Italy.
authors: Maria
authors: M
authors: Banci
authors: Fulvia Gloria-Bottini, Patrizia Saccucci, Egidio Bottini, Department of Biomedicine and Prevention, School of Medicine, University of Rome Tor Vergata, 00133 Rome, Italy.
authors: Paolo
authors: P
authors: Nardi
authors: Fulvia Gloria-Bottini, Patrizia Saccucci, Egidio Bottini, Department of Biomedicine and Prevention, School of Medicine, University of Rome Tor Vergata, 00133 Rome, Italy.
authors: Mattia
authors: M
authors: Scognamiglio
authors: Fulvia Gloria-Bottini, Patrizia Saccucci, Egidio Bottini, Department of Biomedicine and Prevention, School of Medicine, University of Rome Tor Vergata, 00133 Rome, Italy.
authors: Antonio
authors: A
authors: Pellegrino
authors: Fulvia Gloria-Bottini, Patrizia Saccucci, Egidio Bottini, Department of Biomedicine and Prevention, School of Medicine, University of Rome Tor Vergata, 00133 Rome, Italy.
authors: Egidio
authors: E
authors: Bottini
authors: Fulvia Gloria-Bottini, Patrizia Saccucci, Egidio Bottini, Department of Biomedicine and Prevention, School of Medicine, University of Rome Tor Vergata, 00133 Rome, Italy.
authors: Luigi
authors: L
authors: Chiariello
keywords: Acid phosphatse locus 1; Adenosine deaminase 2; Coronary artery disease; PTPN22; p53 codon 72