title: Multilocus genotyping of Giardia duodenalis in dairy cattle in Henan, China.
abstract: Giardia duodenalis is a common and widespread intestinal protozoan parasite of both humans and animals. Previous epidemiological and molecular studies have identified Giardia infections in different animals and humans, but only limited information is available about the occurrence and genotypes of Giardia in cattle in China. In this study, we determined the occurrence of giardiasis and genetically characterized G. duodenalis in dairy cattle in Henan Province, central China. The overall prevalence of G. duodenalis was 7.2% (128/1777) on microscopic analysis, with the highest infection rate (22.7%) in calves aged less than 1 month. G. duodenalis assemblages and subtypes were identified with multilocus genotyping based on the SSU rRNA, β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes. Two assemblages were detected in the successfully sequenced samples: assemblage A (n = 58), assemblage E (n = 21), with a mixed E and A assemblage (n = 2). Four novel subtypes of the gdh gene and seven of the bg gene were found among the G. duodenalis assemblage E isolates. Using the nomenclature for the multilocus genotype (MLG) model, nine novel multilocus genotypes E (MLGs E1-E9) and three MLGs A (a novel subtype AI, previously detected subtype AII-1, and a combination of both) were identified. MLG AII-1 identified in this study may be an important zoonotic subtype. The dairy cattle in Henan are a potential public health concern.
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. China; Department of Animal Science, Henan Vocational College of Agriculture, Zhongmu, Henan Province, P. R. China; College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China.
authors: Haiyan
authors: H
authors: Wang
authors: College of Veterinary Medicine, Northwest A&F University, Yangling, Shanxi Province, P. R. China.
authors: Guanghui
authors: G
authors: Zhao
authors: Department of Animal Science, Henan Vocational College of Agriculture, Zhongmu, Henan Province, P. R. China.
authors: Gongyi
authors: G
authors: Chen
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. China.
authors: Fuchun
authors: F
authors: Jian
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. China.
authors: Sumei
authors: S
authors: Zhang
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. China.
authors: Chao
authors: C
authors: Feng
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. China.
authors: Rongjun
authors: R
authors: Wang
authors: Department of Animal Science, Henan Vocational College of Agriculture, Zhongmu, Henan Province, P. R. China.
authors: Jinfeng
authors: J
authors: Zhu
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. China.
authors: Haiju
authors: H
authors: Dong
authors: Department of Animal Science, Henan Vocational College of Agriculture, Zhongmu, Henan Province, P. R. China.
authors: Jun
authors: J
authors: Hua
authors: College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China.
authors: Ming
authors: M
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. China.
authors: Longxian
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D002681:China / Q000453:epidemiology; D017125:Genes, Protozoan; D005838:Genotype; D005843:Geography; D016829:Giardia lamblia / Q000145:classification* / Q000235:genetics*; D005873:Giardiasis / Q000662:veterinary*; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D011110:Polymorphism, Genetic; D015995:Prevalence