title: Surface plasmon resonance-based infrared biosensor for cell studies with simultaneous control.
abstract: We report a label-free infrared surface plasmon biosensor with a double-chamber flow cell for continuous monitoring of morphological changes in cell culture exposed to various stimuli. In this technique, the monolayer of cultured cells is divided into two halves by a barrier, allowing the treatment of one half while the other serves as control. We demonstrate the advantages of this setup in test experiments that track kinetics of the IEC-18 cell layer response to variations in extracellular Ca2+ concentration. The sensitivity of the presented method was found to be an order of magnitude higher compared to the single-chamber biosensor.
authors: Hebrew University of Jerusalem, The Racah Institute of Physics, 91904 Jerusalem, Israel.
authors: Alexander
authors: A
authors: Zilbershtein
authors: Hebrew University of Jerusalem, School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, P.O. Box 12, 76100 Rehovot, Israel.
authors: Amir
authors: A
authors: Bein
authors: Hebrew University of Jerusalem, The Racah Institute of Physics, 91904 Jerusalem, Israel.
authors: Vladislav
authors: V
authors: Lirtsman
authors: Hebrew University of Jerusalem, School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, P.O. Box 12, 76100 Rehovot, Israel.
authors: Betty
authors: B
authors: Schwartz
authors: Hebrew University of Jerusalem, The Racah Institute of Physics, 91904 Jerusalem, Israel.
authors: Michael
authors: M
authors: Golosovsky
authors: Hebrew University of Jerusalem, The Racah Institute of Physics, 91904 Jerusalem, Israel.
authors: Dan
authors: D
authors: Davidov
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002118:Calcium / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D051381:Rats; D012031:Refractometry; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
chemical_list: D002118:Calcium