title: Quantitatively mapping cellular viscosity with detailed organelle information via a designed PET fluorescent probe.
abstract: Viscosity is a fundamental physical parameter that influences diffusion in biological processes. The distribution of intracellular viscosity is highly heterogeneous, and it is challenging to obtain a full map of cellular viscosity with detailed organelle information. In this work, we report 1 as the first fluorescent viscosity probe which is able to quantitatively map cellular viscosity with detailed organelle information based on the PET mechanism. This probe exhibited a significant ratiometric fluorescence intensity enhancement as solvent viscosity increases. The emission intensity increase was attributed to combined effects of the inhibition of PET due to restricted conformational access (favorable for FRET, but not for PET), and the decreased PET efficiency caused by viscosity-dependent twisted intramolecular charge transfer (TICT). A full map of subcellular viscosity was successfully constructed via fluorescent ratiometric detection and fluorescence lifetime imaging; it was found that lysosomal regions in a cell possess the highest viscosity, followed by mitochondrial regions.
authors: 1] Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China [2] State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China [3].
authors: Tianyu
authors: T
authors: Liu
authors: 1] Cavendish Laboratory, Department of Physics, University of Cambridge, UK [2].
authors: Xiaogang
authors: X
authors: Liu
authors: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK.
authors: David R
authors: DR
authors: Spring
authors: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors: Xuhong
authors: X
authors: Qian
authors: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China.
authors: Jingnan
authors: J
authors: Cui
authors: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Zhaochao
authors: Z
authors: Xu
mesh_terms: D000818:Animals; D002460:Cell Line; D018360:Crystallography, X-Ray; D003593:Cytoplasm / Q000737:chemistry*; D004579:Electron Transport; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D015394:Molecular Structure; D015388:Organelles / Q000737:chemistry*; D016716:PC12 Cells; D051381:Rats; D013050:Spectrometry, Fluorescence; D014783:Viscosity
chemical_list: D005456:Fluorescent Dyes