title: Hormonal dynamics during recovery from drought in two Eucalyptus globulus genotypes: from root to leaf.
abstract: Drought is a limiting environmental stress that represents a growing constraint to the forestry sector. Eucalyptus globulus is a widely planted coppice species, which capacity to cope with water deficit has already been described. However, the capacity of this species to recover is still poorly understood. In this study, we aimed to investigate the changes in abscisic acid (ABA), ABA-glucose ester (ABA-GE) and jasmonic acid (JA) content in leaves, xylem sap and roots of two genotypes (AL-10 and AL-18) during rewatering (2 h, 4 h, 24 h, and 168 h), after a drought stress period (0 h). We wished to clarify the role of these hormones in the recovery from drought and to determine whether these hormonal relations were related to specific genotype metabolisms. Our results showed that drought caused an increased in ABA and ABA-GE levels in all analysed plant parts, while JA content decreased in leaves, increased in xylem sap and did not change in roots. Some of these responses were genotype specific. During rewatering, ABA and ABA-GE content decreased in both genotypes and all plant parts, but at different time scales, and JA levels did not greatly change. Again, the genotypes responded differently. Altogether, our results characterised the response pattern of clone AL-10 as more responsive and defended that leaf should be used in preliminary screening methods of stress tolerance. The hormonal dynamics were related to the previously documented responses of these genotypes and sustain further physiological and molecular studies of water stress in this and other tree species.
authors: Department of Biology & CESAM - Centre for Environmental and Marine Studies, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
authors: Barbara
authors: B
authors: Correia
authors: Molecular Ecophysiology Lab., ITQB (Instituto de Tecnologia Química e Biológica), 2780-901 Oeiras, Portugal; Departament de Biologia Vegetal, Facultat de Biologia, University of Barcelona, Avinguda Diagonal 643, 08028 Barcelona, Spain.
authors: Marta
authors: M
authors: Pintó-Marijuan
authors: Department of Biology & CESAM - Centre for Environmental and Marine Studies, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
authors: Bruno B
authors: BB
authors: Castro
authors: Departament de Biologia Vegetal, Facultat de Biologia, University of Barcelona, Avinguda Diagonal 643, 08028 Barcelona, Spain.
authors: Ricard
authors: R
authors: Brossa
authors: Departament de Biologia Vegetal, Facultat de Biologia, University of Barcelona, Avinguda Diagonal 643, 08028 Barcelona, Spain.
authors: Marta
authors: M
authors: López-Carbonell
authors: Department of Biology & CESAM - Centre for Environmental and Marine Studies, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal. Electronic address: gpinto@ua.pt.
authors: Glória
authors: G
authors: Pinto
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D003681:Dehydration / Q000378:metabolism; D005052:Eucalyptus / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D005838:Genotype; D054883:Oxylipins / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid; D000040:Abscisic Acid
keywords: ABA; ABA-GE; JA; PCA; Rehydration; Water stress