title: Chemopreventive effects of rosmarinic acid on rat colon carcinogenesis.
abstract: Rosmarinic acid (RA) is a polyphenolic compound that shows a number of interesting biological activities, such as antiapoptotic, antifibrotic, antioxidant, hepatoprotective, antineurodegenerative, and anti-inflammatory properties. The aim of this study was to investigate the ability of RA to prevent 1,2-dimethylhydrazine (DMH)-induced primary DNA damage and aberrant crypt foci (ACF) in Wistar rat colon. The animals were treated by gavage with doses of 4, 8, and 16 mg/kg body weight/day. Next, the animals received a single subcutaneous injection of 40 mg/kg DMH and were killed 4 h later for the evaluation of DNA damage using the comet assay. In addition, two doses of 40 mg/kg DMH were administered weekly for 2 weeks and the animals were killed 2 weeks after the last injection for the evaluation of ACF formation in rat colon. The results showed that RA exerted no genotoxic/carcinogenic effects. Treatment with different doses of RA combined with DMH led to a significant reduction in the extent of DNA damage and in the frequency of ACF compared with animals treated with DMH alone. These findings suggest that RA reduces DNA damage and suppresses the formation of ACF.
authors: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque, Universitário, São Paulo, Brazil.
authors: Ricardo A
authors: RA
authors: Furtado
authors: Barthira R
authors: BR
authors: Oliveira
authors: Luciana R
authors: LR
authors: Silva
authors: Sabrina S
authors: SS
authors: Cleto
authors: Carla C
authors: CC
authors: Munari
authors: Wilson R
authors: WR
authors: Cunha
authors: Denise C
authors: DC
authors: Tavares
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D058739:Aberrant Crypt Foci / Q000139:chemically induced / Q000473:pathology*; D000230:Adenocarcinoma / Q000139:chemically induced / Q000473:pathology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D063646:Carcinogenesis / Q000139:chemically induced / Q000187:drug effects*; D002273:Carcinogens / Q000633:toxicity; D002934:Cinnamates / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology*; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics; D053630:Depsides / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000097123:Rosmarinic Acid
chemical_list: D019813:1,2-Dimethylhydrazine; D000975:Antioxidants; D002273:Carcinogens; D002934:Cinnamates; D053630:Depsides; D000097123:Rosmarinic Acid