title: Virulence, antibiotic resistance and biogenic amines of bacteriocinogenic lactococci and enterococci isolated from goat milk.
abstract: The present study aimed to investigate the virulence, antibiotic resistance and biogenic amine production in bacteriocinogenic lactococci and enterococci isolated from goat milk in order to evaluate their safety. Twenty-nine bacteriocinogenic lactic acid bacteria (LAB: 11 Lactococcus spp., and 18 Enterococcus spp.) isolated from raw goat milk were selected and subjected to PCR to identify gelE, cylA, hyl, asa1, esp, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc genes. The expression of virulence factors (gelatinase, hemolysis, lipase, DNAse, tyramine, histamine, putrescine) in different incubation temperatures was assessed by phenotypic methods, as well as the resistance to vancomycin, gentamicin, chloramphenicol, ampicillin and rifampicin (using Etest®). The tested isolates presented distinct combinations of virulence related genes, but not necessarily the expression of such factors. The relevance of identifying virulence-related genes in bacteriocinogenic LAB was highlighted, demanding for care in their usage as starter cultures or biopreservatives due to the possibility of horizontal gene transfer to other bacteria in food systems.
authors: Departamento de Veterinária, Universidade Federal de Viçosa, 36570 900 Viçosa, MG, Brazil.
authors: Luana Martins
authors: LM
authors: Perin
authors: Departamento de Veterinária, Universidade Federal de Viçosa, 36570 900 Viçosa, MG, Brazil.
authors: Rodrigo Otávio
authors: RO
authors: Miranda
authors: Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 05508 000 São Paulo, SP, Brazil.
authors: Svetoslav Dimitrov
authors: SD
authors: Todorov
authors: Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 05508 000 São Paulo, SP, Brazil.
authors: Bernadette Dora Gombossy de Melo
authors: BD
authors: Franco
authors: Departamento de Veterinária, Universidade Federal de Viçosa, 36570 900 Viçosa, MG, Brazil. Electronic address: nero@ufv.br.
authors: Luís Augusto
authors: LA
authors: Nero
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D016983:Enterococcus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D020869:Gene Expression Profiling; D006041:Goats; D017045:Lactococcus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001679:Biogenic Amines; D037521:Virulence Factors
keywords: Antibiotic resistance; Bacteriocins; Biogenic amines; Goat milk; Lactic acid bacteria; Virulence