title: A novel miR-219-SMC4-JAK2/Stat3 regulatory pathway in human hepatocellular carcinoma.
abstract: BACKGROUND
  To understand the involvement of structural maintenance of chromosome 4 (SMC4) in the development and progression of hepatocellular carcinoma (HCC).
  
  
  METHODS
  Real-time quantitative PCR and Western Blotting were applied to measure the expression of SMC4 in HCC samples and cell lines. The tumor-promoting effect of SMC4 was determined by WST-1, soft agar colony formation, cell motility and invasion assays. The SMC4 target signal pathway was identified by luciferase reporter and real-time quantitative PCR assays.
  
  
  RESULTS
  The upregulation of SMC4 was frequently detected in HCC samples and cell lines. Functional assays demonstrated that SMC4 could effectively promote tumor cell growth rate, colony formation in soft agar, wound-healing and invasion. Further studies showed that increased miR-219 levels caused a significant decrease in the SMC4 expression, and SMC4 inhibitor downregulated JAK2/Stat3 expression at both the mRNA and protein levels.
  
  
  CONCLUSIONS
  Our findings provide new insight into SMC4 function and the mechanisms of growth and invasion of HCC.
authors: Bo
authors: B
authors: Zhou
authors: Hongxu
authors: H
authors: Chen
authors: Dong
authors: D
authors: Wei
authors: Yi
authors: Y
authors: Kuang
authors: Xiaobiao
authors: X
authors: Zhao
authors: Guangyao
authors: G
authors: Li
authors: Jun
authors: J
authors: Xie
authors: Department of Hepatobiliary Surgery, Daping Hospital and Research Institute of Surgery, The Third Military Medical University, Chongqing, China. chenpingsyd@126.com.
authors: Ping
authors: P
authors: Chen
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D018450:Disease Progression; D056945:Hep G2 Cells; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection; D015854:Up-Regulation
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C578248:MIRN219 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C507924:JAK2 protein, human; D053614:Janus Kinase 2; D000251:Adenosine Triphosphatases; C580922:SMC4 protein, human