title: Heat shock protein 70 from Trichinella spiralis induces protective immunity in BALB/c mice by activating dendritic cells.
abstract: Trichinella spiralis heat shock protein 70 (Ts-Hsp70) is a protective antigen that induces partial protective immunity against T. spiralis infection in mice. To determine whether dendritic cells are involved in the mechanism responsible for the protection induced by Ts-Hsp70, mouse bone marrow-derived dendritic cells (DCs) were incubated with recombinant Ts-Hsp70 (rTs-Hsp70), and the DC-secreted cytokines and expressed surface markers were measured. The results demonstrated that rTs-Hsp70 activated DC maturation that was characterized by the secretion of IL-1β, IL-12p70, TNF-α, and IL-6 and the increased surface expression of CD11c, MHC II, CD40, CD80, and CD86. The rTs-Hsp70-activated DCs enabled the stimulation, proliferation and secretion of Th1/2 cytokines (i.e., INF-γ, IL-2, IL-4 and IL-6) in CD4(+) T cells from T. spiralis-infected mice. The mice that received rTs-Hsp70-activated DCs exhibited a 38.4% reduction in muscle larvae upon larval challenge with T. spiralis compared to the group that received PBS-incubated DCs. This partial protection was correlated with Th1 and Th2 mixed anti-Ts-Hsp70-specific immune responses that included high titers of total IgG, IgG1 and IgG2a and increased levels of Th1/2 cytokines (i.e., IFN-γ, IL-2, IL-4, IL-6). These results indicate that the rTs-Hsp70-induced protective immunity was mediated by the activation of the DCs and that rTs-Hsp70-loaded DCs could be an alternative vaccine approach against trichinellosis.
authors: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Lei
authors: L
authors: Fang
authors: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Liang
authors: L
authors: Sun
authors: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Jing
authors: J
authors: Yang
authors: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Yuan
authors: Y
authors: Gu
authors: Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, USA.
authors: Bin
authors: B
authors: Zhan
authors: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Jingjing
authors: J
authors: Huang
authors: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China. Electronic address: zhuxping@ccmu.edu.cn.
authors: Xinping
authors: X
authors: Zhu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000947:Antigens, Helminth / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000469:parasitology; D059208:Parasite Load; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D000947:Antigens, Helminth; D016207:Cytokines; D018840:HSP70 Heat-Shock Proteins; D000949:Histocompatibility Antigens Class II
keywords: Dendritic cells; Heat shock protein; Trichinella spiralis; Vaccine