title: Bioreducible cross-linked nanoshell enhances gene transfection of polycation/DNA polyplex in vivo.
abstract: In this study, we have prepared a self-cross-linking PEG-based branched polymer, which easily forms a bioreducible nanoshell around polyplexes of cationic polymer and DNA, simply via heating the polyplex dispersions in the presence of this self-cross-linking branched polymer. This nanoshell can prevent the polyplex from dissociation and aggregation in physiological fluids without inhibiting the electrostatic interactions between the polymer and DNA. Furthermore, glutathione (GSH) can act as a stimulus to open the nanoshell after it has entered the cell. The polyplexes coated with the bioreducible nanoshell show an obvious enhancement in gene transfection in vivo compared with bare polyplexes.
authors: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China , Jinzhai Road 96, Hefei 230026, Anhui, China.
authors: Ji-Gang
authors: JG
authors: Piao
authors: Sheng-Gang
authors: SG
authors: Ding
authors: Lu
authors: L
authors: Yang
authors: Chun-Yan
authors: CY
authors: Hong
authors: Ye-Zi
authors: YZ
authors: You
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D051379:Mice; D057145:Nanoshells / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D055672:Static Electricity; D014162:Transfection / Q000379:methods*
chemical_list: D011092:Polyethylene Glycols; D004247:DNA