title: Intracellular Zn(2+) signaling in the dentate gyrus is required for object recognition memory.
abstract: The role of perforant pathway-dentate granule cell synapses in cognitive behavior was examined focusing on synaptic Zn(2+) signaling in the dentate gyrus. Object recognition memory was transiently impaired when extracellular Zn(2+) levels were decreased by injection of clioquinol and N,N,N',N'-tetrakis-(2-pyridylmethyl) ethylendediamine. To pursue the effect of the loss and/or blockade of Zn(2+) signaling in dentate granule cells, ZnAF-2DA (100 pmol, 0.1 mM/1 µl), an intracellular Zn(2+) chelator, was locally injected into the dentate molecular layer of rats. ZnAF-2DA injection, which was estimated to chelate intracellular Zn(2+) signaling only in the dentate gyrus, affected object recognition memory 1 h after training without affecting intracellular Ca(2+) signaling in the dentate molecular layer. In vivo dentate gyrus long-term potentiation (LTP) was affected under the local perfusion of the recording region (the dentate granule cell layer) with 0.1 mM ZnAF-2DA, but not with 1-10 mM CaEDTA, an extracellular Zn(2+) chelator, suggesting that the blockade of intracellular Zn(2+) signaling in dentate granule cells affects dentate gyrus LTP. The present study demonstrates that intracellular Zn(2+) signaling in the dentate gyrus is required for object recognition memory, probably via dentate gyrus LTP expression.
authors: Department of Neurophysiology, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan; Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.
authors: Atsushi
authors: A
authors: Takeda
authors: Haruna
authors: H
authors: Tamano
authors: Taisuke
authors: T
authors: Ogawa
authors: Shunsuke
authors: S
authors: Takada
authors: Masatoshi
authors: M
authors: Nakamura
authors: Hiroaki
authors: H
authors: Fujii
authors: Masaki
authors: M
authors: Ando
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002491:Central Nervous System Agents / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D007464:Clioquinol / Q000494:pharmacology; D018891:Dentate Gyrus / Q000187:drug effects / Q000502:physiology*; D005110:Extracellular Space / Q000378:metabolism; D007477:Ions / Q000378:metabolism; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009483:Neuropsychological Tests; D017208:Rats, Wistar; D021641:Recognition, Psychology / Q000187:drug effects / Q000502:physiology*; D015398:Signal Transduction; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D046509:Tissue Culture Techniques; D015032:Zinc / Q000378:metabolism*
chemical_list: D002491:Central Nervous System Agents; D002614:Chelating Agents; D007477:Ions; D007464:Clioquinol; D015032:Zinc; D002118:Calcium
keywords: Zn2+ signaling; ZnAF-2DA; clioquinol; cognition; dentate gyrus; zinc chelator