title: Proteomic analysis of GPI-anchored membrane proteins.
abstract: Glycosyl-phosphatidyl-inositol-anchored proteins (GPI-APs) represent a subset of post-translationally modified proteins that are tethered to the outer leaflet of the plasma membrane via a C-terminal GPI anchor. GPI-APs are found in a variety of eukaryote species, from pathogenic microorganisms to humans. GPI-APs confer important cellular functions as receptors, enzymes and scaffolding molecules. Specific enzymes and detergent extraction methods combined with separation technologies and mass spectrometry permit proteomic analysis of GPI-APs from plasma membrane preparations to reveal cell-type specific surface molecules, candidate biomarkers and potential therapeutic targets.:
authors: Protein Research Group, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230, Odense M, Denmark.
authors: Hye Ryung
authors: HR
authors: Jung
authors: Protein Research Group, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230, Odense M, Denmark. Electronic address:jenseno@bmb.sdu.dk.
authors: Ole Nørregaard
authors: ON
authors: Jensen