title: A developmental hepatotoxicity study of dietary bisphenol A in Sparus aurata juveniles.
abstract: Previous studies in rats have indicated that a diet enriched with Bisphenol A adversely effects metabolism and reproductive success. In rats exposed to BPA by maternal gavage, alteration in the developmental programming, higher obesity rates and reproductive anomalies were induced. Starting with this evidence, the aim of this study was to provide important insights on the effects induced by a BPA enriched diet, on the reproductive physiology and metabolism of juvenile fish, simulating the scenario occurring when wild fish fed on prey contaminated with environmental BPA. Seabream was chosen as model, as it is one of the primary commercial species valued by consumers and these results could provide important findings on adverse effects that could be passed on to humans by eating contaminated fish. A novel method for measuring BPA in the food and water by affinity chromatography was developed. Analysis of signals involved in reproduction uncovered altered levels of vtg and Zp, clearly indicating the estrogenic effect of BPA. Similarly, BPA up-regulated catd and era gene expression. A noteworthy outcome from this study was the full length cloning of two vtg encoding proteins, namely vtgA and vtgB, which are differently modulated by BPA. Cyp1a1 and EROD activity were significantly downregulated, confirming the ability of estrogenic compounds to inhibit the detoxification process. GST activity was unaffected by BPA contamination, while CAT activity was down regulated. These results collectively confirm the estrogenic effect of BPA and provide additional characterization of novel vtg genes in Sparus aurata.
authors: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, 60131 Ancona, Italy.
authors: Francesca
authors: F
authors: Maradonna
authors: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, 60131 Ancona, Italy.
authors: Valentina
authors: V
authors: Nozzi
authors: Dipartimento di Biologia, Università di Padova, 35131 Padova, Italy.
authors: Luisa
authors: L
authors: Dalla Valle
authors: Dipartimento di Scienze della Terra, dell'Ambiente e della Vita, Università di Genova, 16132 Genova, Italy; INBB Consorzio Interuniversitario di Biosistemi e Biostrutture, 00136 Roma, Italy.
authors: Ilaria
authors: I
authors: Traversi
authors: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, 60131 Ancona, Italy.
authors: Giorgia
authors: G
authors: Gioacchini
authors: Dipartimento di Biologia, Università di Padova, 35131 Padova, Italy.
authors: Francesca
authors: F
authors: Benato
authors: Dipartimento di Biologia, Università di Padova, 35131 Padova, Italy.
authors: Elisa
authors: E
authors: Colletti
authors: INBB Consorzio Interuniversitario di Biosistemi e Biostrutture, 00136 Roma, Italy; Dipartimento di Chimica, Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Portici (NA), Italy.
authors: Pasquale
authors: P
authors: Gallo
authors: Dipartimento di Chimica, Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Portici (NA), Italy.
authors: Ilaria
authors: I
authors: Di Marco Pisciottano
authors: INBB Consorzio Interuniversitario di Biosistemi e Biostrutture, 00136 Roma, Italy.
authors: Damiano G
authors: DG
authors: Mita
authors: Department of Medicine, Medical University of South Carolina, Charleston, SC, USA; Computational Science Research Center Biomedical Informatics Research Center, San Diego State University, San Diego, CA, USA.
authors: Gary
authors: G
authors: Hardiman
authors: Dipartimento di Scienze della Terra, dell'Ambiente e della Vita, Università di Genova, 16132 Genova, Italy; INBB Consorzio Interuniversitario di Biosistemi e Biostrutture, 00136 Roma, Italy.
authors: Alberta
authors: A
authors: Mandich
authors: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, 60131 Ancona, Italy; INBB Consorzio Interuniversitario di Biosistemi e Biostrutture, 00136 Roma, Italy. Electronic address: o.carnevali@univpm.it.
authors: Oliana
authors: O
authors: Carnevali
mesh_terms: D000595:Amino Acid Sequence; D000821:Animal Feed / Q000009:adverse effects; D000818:Animals; D017756:Aquaculture; D001559:Benzhydryl Compounds / Q000008:administration & dosage / Q000633:toxicity*; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000662:veterinary*; D004305:Dose-Response Relationship, Drug; D004527:Egg Proteins / Q000235:genetics / Q000378:metabolism; D052244:Endocrine Disruptors / Q000008:administration & dosage / Q000633:toxicity*; D005393:Fish Diseases / Q000139:chemically induced* / Q000378:metabolism; D029941:Fish Proteins / Q000819:agonists / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005506:Food Contamination; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010636:Phenols / Q000008:administration & dosage / Q000633:toxicity*; D020033:Protein Isoforms / Q000819:agonists / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D021541:Sea Bream; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014819:Vitellogenins / Q000819:agonists / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000072216:Zona Pellucida Glycoproteins; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D015415:Biomarkers; D004527:Egg Proteins; D052244:Endocrine Disruptors; D029941:Fish Proteins; D008562:Membrane Glycoproteins; D010636:Phenols; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; D014819:Vitellogenins; D000072216:Zona Pellucida Glycoproteins; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: CAT; Diet; EROD; Endocrine disruptors; GST; Zp; catd; erα