title: Differentiation-stimulating potency of differentiated HL60 cells after drug treatment.
abstract: Differentiation therapy in the treatment of leukemia is often hampered by limitations on using certain pharmaceutical regents or on the required doses due to various reasons, such as drug-resistance and retinoic acid syndrome. To circumvent these problems, a strategy might be developed on the basis of the ability of drug-differentiated cells to stimulate differentiation in leukemia cells. Using the promyelocytic leukemia cell line HL60 as a cell model, we assessed the differentiation-stimulating potency of differentiated granulocytes and monocytes/macrophages after treatments with all-trans retinoic acid (ATRA) and 12-O-tetradecanoylphorbol-13-acetate (TPA), respectively. ATRA- and TPA-differentiated cells were able to stimulate differentiation in fresh HL60 cells, accompanied by inhibition on cell growth to various extents. The differentiated cells of the second generation, especially those originated from TPA treatment, were as potent as the drugs themselves in stimulating differentiation in fresh HL60 cells. On the basis of "differentiation induced by differentiated cells", we explored the feasibility of ex vivo therapy.
authors: Department of Chemical Biology, Peking University School of Pharmaceutical Sciences, 38, Xueyuan Road, Beijing 100191, PR China.
authors: Cong
authors: C
authors: Wang
authors: Department of Chemical Biology, Peking University School of Pharmaceutical Sciences, 38, Xueyuan Road, Beijing 100191, PR China.
authors: Qun
authors: Q
authors: Zhang
authors: Department of Chemical Biology, Peking University School of Pharmaceutical Sciences, 38, Xueyuan Road, Beijing 100191, PR China.
authors: Bao-Di
authors: BD
authors: Gou
authors: Department of Chemical Biology, Peking University School of Pharmaceutical Sciences, 38, Xueyuan Road, Beijing 100191, PR China. Electronic address: tlzhang@bjmu.edu.cn.
authors: Tian-Lan
authors: TL
authors: Zhang
authors: Department of Chemical Biology, Peking University School of Pharmaceutical Sciences, 38, Xueyuan Road, Beijing 100191, PR China.
authors: Kui
authors: K
authors: Wang
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D018920:Coculture Techniques; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate
keywords: Cell-induced differentiation; Drug-differentiated cell; Leukemia; Phorbol ester; Retinoic acid; Tetradecanoylphorbol acetate