title: Effect of growth factors and pro-inflammatory cytokines by the collagen biocomposite dressing material containing Macrotyloma uniflorum plant extract-In vivo wound healing.
abstract: Open burn wounds require proper dressings for faster healing and to prevent infection. In the present study, a wound dressing material in sheet form, containing fish scale collagen (FSC), physiologically clotted fibrin (PCF) and Macrotyloma uniflorum plant extract (MPE) was applied on the experimental wounds of rats. It was found that MPE accelerated wound healing, by suppressing the cyclooxygenase-2 (COX-2) and inducible nitric oxide synthases (iNOS) expressions thereby reduced inflammation. It has influenced the regulation of growth factors such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF) and transforming growth factor (TGF-β). The biocomposite sheet has enhanced collagen synthesis and down regulated the matrix metalloproteinases (MMPs), thereby helped faster healing of wounds. The results suggest that the incorporation of MPE played an important role in enhancing the wound healing rate. The FSC-PCF biosheet containing MPE may be further tried on the clinical wounds of small and large animals before application on to humans.
authors: Bio-Products Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, Tamilnadu, India.
authors: Thangavelu
authors: T
authors: Muthukumar
authors: Department of Biochemistry, School of Life Sciences, University of Madras, Guindy Campus, Chennai 600025, Tamilnadu, India.
authors: Kannan
authors: K
authors: Anbarasu
authors: Department of Biochemistry, School of Life Sciences, University of Madras, Guindy Campus, Chennai 600025, Tamilnadu, India.
authors: Dharmalingam
authors: D
authors: Prakash
authors: Bio-Products Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, Tamilnadu, India. Electronic address: sastrytp@hotmail.com.
authors: Thotapalli Parvathaleswara
authors: TP
authors: Sastry
mesh_terms: D000818:Animals; D001458:Bandages; D003094:Collagen / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D005399:Fishes; D020869:Gene Expression Profiling; D006595:Hexosamines / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017208:Rats, Wistar; D013718:Tensile Strength / Q000187:drug effects; D014574:Uronic Acids / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D016207:Cytokines; D006595:Hexosamines; D018836:Inflammation Mediators; D036341:Intercellular Signaling Peptides and Proteins; D010936:Plant Extracts; D012333:RNA, Messenger; D014574:Uronic Acids; D003094:Collagen; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D006909:Hydroxyproline
keywords: Collagen; Cyclooxygenase; Cytokine; Growth factors; Matrix metalloproteinase; Wound healing