title: LC/MS/MS determination and pharmacokinetic studies of six compounds in rat plasma following oral administration of the single and combined extracts of Eucommia ulmoides and Dipsacus asperoides.
abstract: OBJECTIVE
  To establish and apply a new LC/MS/MS method for the simultaneous, quantitative determination of six ingredients, aucubin (AU), geniposide (GP), geniposidic acid (GPA), pinoresinol diglucoside (PDG), secologanin (SLG), and loganin (LG) in single and combined extracts of Eucommia ulmoides and Dipsacus asperoides.
  
  
  METHODS
  Using the LC/MS/MS-ESI(-)-MRM mode to detect the six compounds, chromatographic separation was achieved on an Agilent Eclipse plus C18 column, and the mobile phase consisted of solvent A (CH3CN) and solvent B (H2O containing 0.01% CH3COOH V/V).
  
  
  RESULTS
  This method was successfully applied to quantify the six compounds in rat plasma after oral administration, and showed good precision, accuracy, reproducibility, and linear regression (r(2)>0.99).
  
  
  CONCLUSIONS
  The results showed that following the use of the two medicinal plants, for AU and GP, the values of Cmax markedly increased, and the values of cmax markedly decreased. It was found that the compatibility of the medicinal plants might affect their pharmacokinetic properties of their constituents.
authors: Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China.
authors: Yu-Xing
authors: YX
authors: Huang
authors: Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Er-Wei
authors: EW
authors: Liu
authors: Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Lei
authors: L
authors: Wang
authors: Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Yan
authors: Y
authors: Huo
authors: Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Qiang
authors: Q
authors: Wang
authors: Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Olajide
authors: O
authors: Olaleye
authors: Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Tao
authors: T
authors: Wang
authors: Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. Electronic address: gaoxiumei1984@hotmail.com.
authors: Xiu-Mei
authors: XM
authors: Gao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D049917:Dipsacaceae / Q000737:chemistry*; D004338:Drug Combinations; D031283:Eucommiaceae / Q000737:chemistry*; D005260:Female; D057888:Iridoid Glucosides / Q000097:blood / Q000493:pharmacokinetics; D039823:Iridoids / Q000097:blood / Q000493:pharmacokinetics*; D017705:Lignans / Q000097:blood / Q000493:pharmacokinetics*; D010936:Plant Extracts / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D004338:Drug Combinations; D057888:Iridoid Glucosides; D039823:Iridoids; D017705:Lignans; D010936:Plant Extracts; C013200:pinoresinol diglucoside
keywords: Compatibility; Dipsacus asperoides; Eucommia ulmoides; LC/MS/MS; Pharmacokinetics