title: Single-step selection of bivalent aptamers validated by comparison with SELEX using high-throughput sequencing.
abstract: The identification of nucleic acid aptamers would be advanced if they could be obtained after fewer rounds of selection and amplification. In this paper the identification of bivalent aptamers for thrombin by SELEX and single-step selection are compared using next generation sequencing and motif finding informatics. Results show that similar aptamers are identified by both methods. This is significant because it shows that next generation sequencing and motif finding informatics have the potential to simplify the selection of aptamers by avoiding multiple rounds of enzymatic transcription and amplification.
authors: Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom.
authors: Robert
authors: R
authors: Wilson
authors: Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom.
authors: Christian
authors: C
authors: Bourne
authors: Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom.
authors: Roy R
authors: RR
authors: Chaudhuri
authors: Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom.
authors: Richard
authors: R
authors: Gregory
authors: Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom.
authors: John
authors: J
authors: Kenny
authors: Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom.
authors: Andrew
authors: A
authors: Cossins
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D020134:Catalytic Domain; D019295:Computational Biology; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008958:Models, Molecular; D059372:Nucleotide Motifs; D052156:SELEX Aptamer Technique / Q000379:methods*; D017422:Sequence Analysis, DNA; D013917:Thrombin / Q000737:chemistry / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D013917:Thrombin