title: Diosmetin ameliorates the severity of cerulein-induced acute pancreatitis in mice by inhibiting the activation of the nuclear factor-κB.
abstract: Diosmetin (3', 5, 7-trihydroxy-4'-methoxyflavone), the aglycone part of the flavonoid glycosides diosmin occurs naturally in citrus fruit, was considered to exhibit anti-inflammatory and antioxidant properties. Our study aimed to investigate the effect of diosmetin in a murine model of cerulein-induced acute pancreatitis (AP). Experimental AP was induced in mice by seven intraperitoneal injection of cerulein (50 ug/kg) at hourly intervals. Diosmetin (100 mg/kg) or vehicle was pretreated 2 h before the first cerulein injection. After 6 h, 9 h, 12 h of the first cerulein injection, the severity of acute pancreatitis was evaluated biochemically and morphologically. Pretreatment with diosmetin significantly reduced serum levels of amylase and lipase; the histological injury; the secretion of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6; myeloperoxidase (MPO) activity, trypsinogen activation peptide (TAP) level, the expression of inducible nitric oxide synthase (iNOS); and the nuclear factor (NF)-κB activation in cerulein-induced AP. This study showed that administration of diosmetin demonstrated a beneficial effect on the course of cerulein-induced AP in mice. Therefore, diosmetin may become a new therapeutic agent in future clinical trials for treatment of AP.
authors: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors: Ge
authors: G
authors: Yu
authors: Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine 100 Haining Road, Hongkou District, Shanghai 200080, People's Republic of China.
authors: Rong
authors: R
authors: Wan
authors: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors: Guojian
authors: G
authors: Yin
authors: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors: Jie
authors: J
authors: Xiong
authors: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors: Yanling
authors: Y
authors: Hu
authors: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors: Miao
authors: M
authors: Xing
authors: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors: Xiaofeng
authors: X
authors: Cang
authors: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors: Yuting
authors: Y
authors: Fan
authors: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors: Wenqin
authors: W
authors: Xiao
authors: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors: Lei
authors: L
authors: Qiu
authors: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China ; Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine 100 Haining Road, Hongkou District, Shanghai 200080, People's Republic of China.
authors: Xingpeng
authors: X
authors: Wang
authors: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China ; Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine 100 Haining Road, Hongkou District, Shanghai 200080, People's Republic of China.
authors: Guoyong
authors: G
authors: Hu
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000208:Acute Disease; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002108:Ceruletide; D019610:Cytoprotection; D004195:Disease Models, Animal; D005419:Flavonoids / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D012720:Severity of Illness Index; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D005419:Flavonoids; D018836:Inflammation Mediators; D016328:NF-kappa B; D002108:Ceruletide; C039602:diosmetin
keywords: Acute pancreatitis; NF-κB; cytokine; diosmetin; iNOS; inflammation