title: ClonorESTdb: a comprehensive database for Clonorchis sinensis EST sequences.
abstract: BACKGROUND
  Clonorchiasis, which is primarily caused by liver fluke (Platyhelminthes), is a fatal infectious disease that is mainly associated with bile duct malignancy and the subsequent development of cholangiocarcinoma. Thus, a genomic approach now represents an important step to further our knowledge of biology and the pathology of these parasites. The results of expressed sequence tags (ESTs) sequencing need to be well organized into databases to provide an integrated set of tools and functional information.
  
  
  RESULTS
  Here, the ClonorESTdb database represents a collection of Clonorchis sinensis ESTs that is intended as a resource for parasite functional genomics. A total of 55,736 successful EST sequences, which are cleaned and clustered into non-redundant 13,305 C. sinensis assembled EST sequences (6,497 clusters and 6,808 singletons), were obtained from three in-house prepared cDNA libraries of C. sinensis at different developmental stages. The assembled consensus sequences were annotated using the BLAST algorithm or/and hmm against NCBI NR, UniProt, KEGG and InterProScan. The ClonorESTdb database provides functional annotation, their expression profiles, tandem repeats and putative single nucleotide polymorphisms with utility tools such as local BLAST search and text retrieval.
  
  
  CONCLUSIONS
  This resource enables the researcher to identify and compare expression signatures under different biological stages and promotes ongoing parasite drug and vaccine development and biological research.Database URL:http://pathod.cdc.go.kr/clonorestdb/.
authors: Dae-Won
authors: DW
authors: Kim
authors: Won Gi
authors: WG
authors: Yoo
authors: Sanghyun
authors: S
authors: Lee
authors: Myoung-Ro
authors: MR
authors: Lee
authors: Yu-Jung
authors: YJ
authors: Kim
authors: Shin-Hyeong
authors: SH
authors: Cho
authors: Division of Malaria and Parasitic Diseases, Centre for Immunology and Pathology, Korea National Institute of Health, Chungbuk 363-951, Republic of Korea. leewonja@gmail.com.
authors: Won-Ja
authors: WJ
authors: Lee
authors: Jung-Won
authors: JW
authors: Ju
mesh_terms: D000818:Animals; D003004:Clonorchis sinensis / Q000235:genetics* / Q000254:growth & development; D019295:Computational Biology / Q000379:methods; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015723:Gene Library; D023281:Genomics / Q000379:methods / Q000706:statistics & numerical data; D016247:Information Storage and Retrieval / Q000379:methods; D020407:Internet; D008018:Life Cycle Stages / Q000235:genetics; D020641:Polymorphism, Single Nucleotide