title: Biological effects of individually synthesized TNF-binding domain of variola virus CrmB protein.
abstract: The biological characteristics of a 17-kDa protein synthesized in bacterial cells, a TNF-binding domain (VARV-TNF-BP) of a 47-kDa variola virus CrmB protein (VARV-CrmB) consisting of TNF-binding and chemokine-binding domains, were studied. Removal of the C-terminal chemokine-binding domain from VARV-CrmB protein was inessential for the efficiency of its inhibition of TNF cytotoxicity towards L929 mouse fibroblast culture and for TNF-induced oxidative metabolic activity of mouse blood leukocytes. The results of this study could form the basis for further studies of VARV-TNF-BP mechanisms of activity for prospective use in practical medicine.
authors: Research Institute of Clinical Immunology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia, tsdon@mail.ru.
authors: D D
authors: DD
authors: Tsyrendorzhiev
authors: I A
authors: IA
authors: Orlovskaya
authors: S V
authors: SV
authors: Sennikov
authors: T V
authors: TV
authors: Tregubchak
authors: I P
authors: IP
authors: Gileva
authors: M D
authors: MD
authors: Tsyrendorzhieva
authors: S N
authors: SN
authors: Shchelkunov
mesh_terms: D000818:Animals; D002460:Cell Line; D051379:Mice; D010084:Oxidation-Reduction / Q000187:drug effects; D011485:Protein Binding; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism* / Q000633:toxicity; D012901:Variola virus / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D014764:Viral Proteins