title: Thymosin beta 10 correlates with lymph node metastases of papillary thyroid carcinoma.
abstract: BACKGROUND
  Thymosin beta 10 (TMSB10) has recently been recognized as being an important player in the metastatic cascade including tumor angiogenesis, invasion, and metastasis. However, a role for this protein in papillary thyroid carcinoma (PTC) has not yet been established.
  
  
  METHODS
  Real-time polymerase chain reaction was used to examine the expression of TMSB10 messenger RNA in 36 cases of thyroid tissue samples: normal thyroid, PTC without lymph node metastases (LNM) and PTC with LNM (n = 12 cases in each subgroup). For immunohistochemistry, 130 patients with PTC were selected during the period of 2004-2005, 91 with and 39 without LNM. Statistical analysis was applied to evaluate the correlation between TMSB10 expression and LNM of PTC.
  
  
  RESULTS
  By real-time polymerase chain reaction analysis, the expression of TMSB10 messenger RNA in normal thyroid tissue, PTC without LNM, and PTC with LNM tissue were significantly different (P < 0.0001). On immunohistochemistry analysis of 130 patients with PTC, in which 91 cases had cervical LNM and 69 cases had central neck LNM, high expression levels for TMSB10 were more common in patients with cervical LNM compared with patients without (81% versus 33%, P < 0.001). Similarly, high expression levels of TMSB10 were more common in patients with central neck LNM compared with those without (87.0% versus 44.3%, P < 0.001).
  
  
  CONCLUSIONS
  High expression levels of TMSB10 correlated with LNM in PTC, especially in the central neck region. Patients with PTC with low levels of TMSB10 expression may be unlikely to have central neck LNM and could therefore avoid prophylactic central neck dissection.
authors: Department of Head and Neck Surgery, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China; State Key Laboratory of Oncology in South China, Guangzhou, P. R. China; Collaborative Innovation Center for Cancer Medicine, Guangzhou, P. R. China.
authors: Xiao-Jun
authors: XJ
authors: Zhang
authors: Department of General Surgery, Jiangmen Central Hospital, Jiangmen, P. R. China.
authors: Yao-Rong
authors: YR
authors: Su
authors: Department of General Surgery, The Fifth People's Hospital of Shenzhen, Shenzhen, P. R. China.
authors: Dan
authors: D
authors: Liu
authors: Department of Thyroid Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, P.R. China.
authors: De-Bin
authors: DB
authors: Xu
authors: Laboratory of Oncology, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China; State Key Laboratory of Oncology in South China, Guangzhou, P. R. China; Collaborative Innovation Center for Cancer Medicine, Guangzhou, P. R. China.
authors: Mu-Sheng
authors: MS
authors: Zeng
authors: Department of Head and Neck Surgery, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China; State Key Laboratory of Oncology in South China, Guangzhou, P. R. China; Collaborative Innovation Center for Cancer Medicine, Guangzhou, P. R. China. Electronic address: chenwk@sysucc.org.cn.
authors: Wen-Kuan
authors: WK
authors: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D002291:Carcinoma, Papillary; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006044:Goiter, Nodular / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D013947:Thymosin / Q000235:genetics* / Q000378:metabolism; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D013947:Thymosin; C039302:thymosin beta(10)
keywords: Central neck dissection; Lymph node metastasis; Papillary thyroid carcinoma; Thymosin beta 10