title: Antiphospholipid antibodies in women undergoing in vitro fertilization treatment: clinical value of IgA anti-β2glycoprotein I antibodies determination.
abstract: Implantation failure could be related to antiphospholipid antibodies (aPL). We retrospectively analyzed the usefulness of aPL determination in women undergoing IVF. Conventional aPL of the antiphospholipid syndrome, lupus anticoagulant (LA), anticardiolipin antibodies (aCL), anti-β2glycoprotein I (a β2GPI) antibodies, and IgG and IgM isotypes as well as IgA isotype were analyzed in women presenting with at least two implantation failures after in vitro fertilization (IVF). In a population of 40 IVF patients, a total prevalence of 20% (8/40) of aPL was found, significantly different from that of the control population (100 healthy blood donors, P < 0.0005). Among the panels of aPL tested, a β2GPI IgA antibodies were the most prevalent (62.5% 5/8), significantly higher in IVF patients (12.5%, 5/40) than in controls (1%, 1/100) (P = 0.01). No difference according to the numbers of IVF attempts and success of embryo implantation was found between aPL positive and negative IVF patients. In contrast, no accomplished pregnancy with full-term live birth was observed in aPL positive IVF patients. Altogether our data led us to propose aPL assessment, in particular a β2GPI IgA antibodies, in support of IVF treated women. In a perspective way, an early aPL detection could be the basis for defining novel therapeutic strategy.
authors: Laboratoire d'Assistance Médicale à la Procréation, Hôpital de la Conception, 147 boulevard Baille, 13005 Marseille, France ; Laboratoire d'Histologie-Embryologie, UFR Médecine, Université Aix-Marseille, 127 boulevard Jean Moulin, Marseille, France.
authors: Odile
authors: O
authors: Paulmyer-Lacroix
authors: Laboratoire d'Immunologie, Hôpital de la Conception, 147 boulevard Baille, 13005 Marseille, France.
authors: Laura
authors: L
authors: Despierres
authors: Department of Gynecology, Obstetric and Reproductive Medicine, Gynepole, AP-HM La Conception University Hospital, 147 bd Baille, 13005 Marseille, France ; Aix Marseille Université, CNRS, IRD, Avignon Université, IMBE UMR 7263, 13397 Marseille, France ; FR CNRS 3098, ECCOREV, 13100 Aix-en-Provence, France.
authors: Blandine
authors: B
authors: Courbiere
authors: Laboratoire d'Immunologie, Hôpital de la Conception, 147 boulevard Baille, 13005 Marseille, France ; INSERM UMRS 1076, UFR Pharmacie, Université Aix-Marseille, 127 boulevard Jean Moulin, 13005 Marseille, France.
authors: Nathalie
authors: 0000-0003-3680-082X
authors: N
authors: Bardin
mesh_terms: D000328:Adult; D017152:Antibodies, Antiphospholipid / Q000097:blood*; D010064:Embryo Implantation; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D008297:Male; D011247:Pregnancy / Q000097:blood*; D011446:Prospective Studies; D012189:Retrospective Studies; D053482:beta 2-Glycoprotein I
chemical_list: D017152:Antibodies, Antiphospholipid; D007070:Immunoglobulin A; D053482:beta 2-Glycoprotein I