title: Serum CD10 is associated with liver metastasis in colorectal cancer.
abstract: BACKGROUND
  Colorectal cancer (CRC) is the third leading cause of cancer death in Japan. CD10 expression is closely associated with liver metastasis. In the present study, we explored the possibility of serum CD10 as a marker of liver metastasis in CRC.
  
  
  METHODS
  BALB/c mouse with subcutaneous tumor of syngeneic CT26 CRC cells were examined serum CD10. In 84 CRC patients and patients undergoing hemodialysis, serum CD10 was examined. CD10 concentration was measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  In a mouse subcutaneous tumor model, serum CD10 correlated with the weight of the tumors. Serum CD10 was examined in 84 patients with CRC. The serum levels of CD10 were higher in patients with more advanced cancer stages. Patients with liver metastasis showed the highest levels of serum CD10 among all patients. Importantly, patients with high serum CD10 levels had metachronous liver metastasis. Healthy volunteers showed low levels of CD10; however, serum CD10 levels in patients undergoing hemodialysis showed levels as high as those with stage II and III CRC. A cutoff of serum CD10 set to >1000 pg/mL showed 70% sensitivity and 93% specificity for liver metastasis in CRC. This cutoff included all cases of metachronous liver metastasis.
  
  
  CONCLUSIONS
  With the exclusion of mimicking factors, serum CD10 levels might serve as a useful marker of synchronous and metachronous liver metastasis in CRC.
authors: Department of Gastroenterological Surgery, Fukuoka University School of Medicine, Fukuoka, Japan.
authors: Takamitsu
authors: T
authors: Sasaki
authors: Department of Molecular Pathology, Nara Medical University, Kashihara, Japan. Electronic address: cooninh@zb4.so-net.ne.jp.
authors: Hiroki
authors: H
authors: Kuniyasu
authors: Department of Molecular Pathology, Nara Medical University, Kashihara, Japan.
authors: Yi
authors: Y
authors: Luo
authors: Department of Molecular Pathology, Nara Medical University, Kashihara, Japan.
authors: Rina
authors: R
authors: Fujiwara
authors: Department of Molecular Pathology, Nara Medical University, Kashihara, Japan.
authors: Misaho
authors: M
authors: Kitayoshi
authors: Department of Molecular Pathology, Nara Medical University, Kashihara, Japan.
authors: Eriko
authors: E
authors: Tanabe
authors: Department of Gastroenterological Surgery, Fukuoka University School of Medicine, Fukuoka, Japan.
authors: Daisuke
authors: D
authors: Kato
authors: Department of Gastroenterological Surgery, Fukuoka University School of Medicine, Fukuoka, Japan.
authors: Satoshi
authors: S
authors: Shinya
authors: Department of Molecular Pathology, Nara Medical University, Kashihara, Japan.
authors: Kiyomu
authors: K
authors: Fujii
authors: Department of Molecular Pathology, Nara Medical University, Kashihara, Japan.
authors: Hitoshi
authors: H
authors: Ohmori
authors: Department of Gastroenterological Surgery, Fukuoka University School of Medicine, Fukuoka, Japan.
authors: Yuichi
authors: Y
authors: Yamashita
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000097:blood* / Q000473:pathology*; D004195:Disease Models, Animal; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood* / Q000556:secondary*; D008807:Mice, Inbred BALB C; D009367:Neoplasm Staging; D015260:Neprilysin / Q000097:blood*; D047368:Tumor Burden
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; D015260:Neprilysin
keywords: CD10; Colorectal cancer; Liver metastasis; Serum marker