title: The regulation of HanA during heterocyst development in cyanobacterium Anabaena sp. PCC 7120.
abstract: In response to deprivation of combined nitrogen, the filamentous cyanobacterium Anabaena sp. strain PCC 7120 develops heterocyst, which is specifically involved in the nitrogen fixation. In this study, we focused on the regulation of HanA, a histone-like protein, in heterocyst development. Electrophoretic mobility shift assay results showed that NtcA, a global nitrogen regulator necessary for heterocyst differentiation, could bind to two NtcA-binding motifs in the hanA promoter region. qPCR results also showed that NtcA may regulate the expression of hanA. By using the hanA promoter-controlled gfp as a reporter gene and performing western blot we found that the amount of HanA in mature heterocysts was decreased gradually.
authors: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
authors: Jing-Jing
authors: JJ
authors: Lu
authors: Lei
authors: L
authors: Shi
authors: Wen-Li
authors: WL
authors: Chen
authors: Li
authors: L
authors: Wang
mesh_terms: D017033:Anabaena / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D009584:Nitrogen / Q000378:metabolism*; D009586:Nitrogen Fixation; D011401:Promoter Regions, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009584:Nitrogen