title: Improved performance of the PacBio SMRT technology for 16S rDNA sequencing.
abstract: Improved sequencing accuracy was obtained with 16S amplicons from environmental samples and a known pure culture when upgraded Pacific Biosciences (PacBio) hardware and enzymes were used for the single molecule, real-time (SMRT) sequencing platform. The new PacBio RS II system with P4/C2 chemistry, when used with previously constructed libraries (Mosher et al., 2013) surpassed the accuracy of Roche/454 pyrosequencing platform. With accurate read lengths of >1400 base pairs, the PacBio system opens up the possibility of identifying microorganisms to the species level in environmental samples.
authors: Stroud Water Research Center, Avondale, PA 19311, United States.
authors: Jennifer J
authors: JJ
authors: Mosher
authors: Pacific Biosciences, Menlo Park, CA 94025, United States.
authors: Brett
authors: B
authors: Bowman
authors: Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States.
authors: Erin L
authors: EL
authors: Bernberg
authors: Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States.
authors: Olga
authors: O
authors: Shevchenko
authors: Stroud Water Research Center, Avondale, PA 19311, United States.
authors: Jinjun
authors: J
authors: Kan
authors: Pacific Biosciences, Menlo Park, CA 94025, United States.
authors: Jonas
authors: J
authors: Korlach
authors: Stroud Water Research Center, Avondale, PA 19311, United States. Electronic address: lakaplan@stroudcenter.org.
authors: Louis A
authors: LA
authors: Kaplan
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D020592:Shewanella / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: 16S; PacBio; SMRT sequencing