title: Concepts in quantitative fluorescence microscopy.
abstract: In recent years, there has been an enormous increase in the publication of spatial and temporal measurements made on fluorescence microscopy digital images. Quantitative fluorescence microscopy is a powerful and important tool in biological research but is also an error-prone technique that requires careful attention to detail. In this chapter, we focus on general concepts that are critical to performing accurate and precise quantitative fluorescence microscopy measurements.
authors: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors: Jennifer C
authors: JC
authors: Waters
authors: Department of Cell and Tissue Biology, University of California, San Francisco, USA.
authors: Torsten
authors: T
authors: Wittmann
mesh_terms: D000465:Algorithms; D002478:Cells, Cultured; D007091:Image Processing, Computer-Assisted / Q000379:methods* / Q000592:standards; D008856:Microscopy, Fluorescence / Q000379:methods / Q000592:standards; D059629:Signal-To-Noise Ratio; D059010:Single-Cell Analysis / Q000379:methods / Q000592:standards
keywords: Fluorescence; Intensity; Measure; Microscopy; Quantify; Quantitate; Quantitation; Resolution; Sampling