title: Serum miR-9 as a prognostic biomarker in patients with osteosarcoma.
abstract: OBJECTIVE
  To quantify microRNA-9 (miR-9) concentrations in the serum of patients with osteosarcoma; to explore its relationship with clinicopathological characteristics and prognosis of osteosarcoma.
  
  
  METHODS
  Serum miR-9 was quantified via real-time reverse transcription-polymerase chain reaction in patients with osteosarcoma and healthy control subjects. Overall survival was evaluated using the Kaplan-Meier method.
  
  
  RESULTS
  Serum miR-9 was significantly upregulated in patients with osteosarcoma (n = 118) compared with healthy control subjects (n = 60); its upregulation was significantly associated with advanced tumour-node-metastasis stage, larger tumour size and presence of distant metastasis. Overall survival duration was significantly shorter in patients with relatively high miR-9 concentrations compared with those with relatively low miR-9 concentrations.
  
  
  CONCLUSIONS
  Serum miR-9 concentrations are significantly increased in patients with osteosarcoma compared with healthy controls. Upregulation of miR-9 is associated with tumour stage, size and metastasis. Serum miR-9 quantification may represent a useful diagnostic and prognostic marker of osteosarcoma.
authors: Department of Ultrasonography, China-Japan Union Hospital of Jilin University, Changchun, China.
authors: Dan
authors: D
authors: Fei
authors: Department of Ultrasonography, China-Japan Union Hospital of Jilin University, Changchun, China.
authors: Yang
authors: Y
authors: Li
authors: Department of Orthopaedics, China-Japan Union Hospital of Jilin University, Changchun, China.
authors: Dongxu
authors: D
authors: Zhao
authors: Department of Ultrasonography, China-Japan Union Hospital of Jilin University, Changchun, China.
authors: Kaijun
authors: K
authors: Zhao
authors: Department of Ultrasonography, China-Japan Union Hospital of Jilin University, Changchun, China.
authors: Lu
authors: L
authors: Dai
authors: Department of Orthopaedics, China-Japan Union Hospital of Jilin University, Changchun, China gaozhongli518@126.com.
authors: Zhongli
authors: Z
authors: Gao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics*; D012516:Osteosarcoma / Q000097:blood* / Q000235:genetics / Q000473:pathology*; D011379:Prognosis; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D005819:Genetic Markers; C541874:MIRN92 microRNA, human; D035683:MicroRNAs
keywords: MicroRNA-9; osteosarcoma; serum