title: Next-generation transcriptome characterization in three Nacella species (Patellogastropoda: Nacellidae) from South America and Antarctica.
abstract: The southern tip of South America and Antarctica are particularly interesting due to many genera and also species currently sharing between both areas. The genus Nacella (Patellogastropoda: Nacellidae) is distributed in different regions of South America and Antarctica living preferentially on rocks and boulders and grazing on algae, diatoms and bacterial films. We described the transcriptomes of three Nacella species, Nacella concinna (Strebel, 1908), inhabiting the Antarctic Peninsula; Nacella magallanica (Gmelin, 1791), from Patagonia and Nacella clypeater (Lesson, 1831), from central Chile. In total, we obtained over 20,000 contigs with an average length of 583bp. Homologous protein coding genes (PCGs) for mitochondrial genome of the three species were characterized and a database of molecular markers was also generated. This study represents the first publicly available report on pyrosequencing data for patellogastropod species, and provides an important comparative resource for studies in ecophysiology and evolutionary adaptation in marine invertebrate species.
authors: UPMC Univ Paris 06, UMR 7144, Equipe ABICE, Station Biologique de Roscoff, 29680 Roscoff, France; CNRS, UMR 7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, 29680 Roscoff, France. Electronic address: gonzalo.fuenzalida.delrio@gmail.com.
authors: Gonzalo
authors: G
authors: Fuenzalida
authors: Laboratorio de Ecología Molecular, Instituto de Ecología y Biodiversidad (IEB), Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras, 3425 Santiago, Chile. Electronic address: epoulin@uchile.cl.
authors: Elie
authors: E
authors: Poulin
authors: Laboratorio de Ecología Molecular, Instituto de Ecología y Biodiversidad (IEB), Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras, 3425 Santiago, Chile. Electronic address: omeuno01@hotmail.com.
authors: Claudio
authors: C
authors: Gonzalez-Wevar
authors: AUSTRAL-omics, Facultad de Ciencias, Universidad Austral de Chile, Isla Teja S/N, Casilla 567, Valdivia, Chile. Electronic address: cristian.molina.quiroz@gmail.com.
authors: Cristian
authors: C
authors: Molina
authors: Instituto de Ciencias Ambientales y Evolutivas, Facultad de Ciencias, Universidad Austral de Chile, Isla Teja S/N, Casilla 567, Valdivia, Chile. Electronic address: leylacardenas@uach.cl.
authors: Leyla
authors: L
authors: Cardenas
mesh_terms: D063147:Animal Distribution; D000818:Animals; D000864:Antarctic Regions; D001118:Argentina; D001483:Base Sequence; D002677:Chile; D020451:Contig Mapping; D017931:DNA Primers / Q000235:genetics; D020224:Expressed Sequence Tags / Q000378:metabolism; D049851:Gastropoda / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D059467:Transcriptome / Q000235:genetics*
chemical_list: D017931:DNA Primers
keywords: Antarctica; Comparative transcriptome; Patellogastropods; Pyrosequencing; South America