title: Fatal Cryptococcus gattii genotype AFLP6/VGII infection in a HIV-negative patient: case report and a literature review.
abstract: Cryptococcus gattii, a species belonging to the Cryptococcus complex which occurs endemically in tropical and subtropical regions, has been reported as a causative agent of cryptococcosis in healthy individuals. We report a case of meningitis in HIV-negative patient from Cuiaba, MT, in the Midwestern region of Brazil. Cryptococcus gattii AFLP6/VGII was isolated from cerebrospinal fluid and molecular typing was performed by URA5-RFLP. The in vitro susceptibility profile was determined using the standard method according to the document M27A3, CLSI 2008. C. gattii AFLP6/VGII was shown to be susceptible to the antifungals tested. Treatment with 0.8 mg/kg of amphotericin B was initiated; however, the patient died 2 days after the onset of therapy.
authors: Laboratório de Micologia, Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil.
authors: Olivia Cometti
authors: OC
authors: Favalessa
authors: Márcia dos Santos
authors: Mdos S
authors: Lázera
authors: Bodo
authors: B
authors: Wanke
authors: Luciana
authors: L
authors: Trilles
authors: Doracilde Terumi
authors: DT
authors: Takahara
authors: Tomoko
authors: T
authors: Tadano
authors: Luciana Basili
authors: LB
authors: Dias
authors: Ananda Castro
authors: AC
authors: Vieira
authors: Glaúcia Vanessa
authors: GV
authors: Novack
authors: Rosane Christine
authors: RC
authors: Hahn
mesh_terms: D000328:Adult; D000935:Antifungal Agents / Q000627:therapeutic use; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D003453:Cryptococcosis / Q000188:drug therapy / Q000382:microbiology*; D056285:Cryptococcus gattii / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D017809:Fatal Outcome; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008581:Meningitis / Q000188:drug therapy / Q000382:microbiology*; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D000935:Antifungal Agents
keywords: Cryptococcus gattii; Neurocryptococcosis; midwestern of Brazil; seronegative for HIV