title: MiR-133b regulates the expression of the Actin protein TAGLN2 during oocyte growth and maturation: a potential target for infertility therapy.
abstract: Infertility is an area of increasing in life science research. Although follicular maturation disorders and anovulation are the primary causations of infertility, its molecular mechanism is not well understood. Recent research has shown that microRNAs (miRNAs) might play an important role in the regulation of ovarian follicle development and maturation. In this study, the expression of miRNAs in metaphase I (MI) oocytes treated with or without insulin-like growth factor 1 (IGF-1) was observed by microRNA microarray analysis. Results show that 145 miRNAs were up-regulated and 200 miRNAs were down-regulated in MI oocytes after IGF-1 treatment. MiR-133b, which was up-regulated more than 30-fold, was chosen for further research. As a potential target of miR133b, transgelin 2 (TAGLN2) gene was down-regulated, at both transcription and translation levels, in miR-133b- over-expressed 293T cells, but TAGLN2 was up-regulated when the expression of miR-133b was inhibited. Furthermore, the expression level of TAGLN2 in the ovaries of 8-week- old mice was higher than that observed in 4-week-old mice. Immunofluorescence experiments showed that TAGLN2 was located in the cytoplasm. In general, our results indicate that miR-133b may play important roles in the growth and maturation of oocytes by regulating its potential target, TAGLN2, at both transcription and translation levels. Therefore, our research provides a potential new target for infertility therapy.
authors: Department of Pathology, University of South China, Hengyang, China; The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
authors: Guohong
authors: G
authors: Xiao
authors: The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors: Chenglai
authors: C
authors: Xia
authors: The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
authors: Jie
authors: J
authors: Yang
authors: The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
authors: Jianqiao
authors: J
authors: Liu
authors: The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
authors: Hongzi
authors: H
authors: Du
authors: The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
authors: Xiangjin
authors: X
authors: Kang
authors: The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
authors: Yuyi
authors: Y
authors: Lin
authors: The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
authors: Ronghua
authors: R
authors: Guan
authors: The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors: Pengke
authors: P
authors: Yan
authors: Department of Pathology, University of South China, Hengyang, China; Center for Life Science, Hunan University of Arts and Science, Changde, China.
authors: Shengsong
authors: S
authors: Tang
mesh_terms: D000818:Animals; D001665:Binding Sites; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D057809:HEK293 Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis / Q000235:genetics; D010053:Ovary / Q000378:metabolism; D021381:Protein Transport; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics
chemical_list: D035683:MicroRNAs; D008840:Microfilament Proteins; C517732:Mirn133 microRNA, mouse; D009124:Muscle Proteins; D012333:RNA, Messenger; C585385:Tagln2 protein, mouse