title: Analysis and Design of Stimulus Response Curves of E. coli.
abstract: Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.
authors: Systems Biotechnology, Technische Universität München, Boltzmannstr. 15, Garching b. München, Germany. a.kremling@tum.de.
authors: Andreas
authors: A
authors: Kremling
authors: University Osnabrück, Barbarastrasse 11, Osnabrück, Germany. anna.goehler@gmx.de.
authors: Anna
authors: A
authors: Goehler
authors: University Osnabrück, Barbarastrasse 11, Osnabrück, Germany. knut.jahreis@biologie.uni-osnabrueck.de.
authors: Knut
authors: K
authors: Jahreis
authors: Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany. markus.nees@mpi-magdeburg.mpg.de.
authors: Markus
authors: M
authors: Nees
authors: Systems Biotechnology, Technische Universität München, Boltzmannstr. 15, Garching b. München, Germany. auerbach@web.de.
authors: Benedikt
authors: B
authors: Auerbach
authors: Hans Knoell Institute, Beutenbergstrasse 11a, Jena, Germany. wolgang.schmidt-heck@hki-jena.de.
authors: Wolfgang
authors: W
authors: Schmidt-Heck
authors: Helmholtz Center for Infection Research, Inhoffenstr. 7, Braunschweig, Germany. koekpinar@iftc.uni-hannover.de.
authors: Oznur
authors: O
authors: Kökpinar
authors: Helmholtz Center for Infection Research, Inhoffenstr. 7, Braunschweig, Germany. Robert.Geffers@helmholtz-hzi.de.
authors: Robert
authors: R
authors: Geffers
authors: Helmholtz Center for Infection Research, Inhoffenstr. 7, Braunschweig, Germany. Ursula.Rinas@helmholtz-hzi.de.
authors: Ursula
authors: U
authors: Rinas
authors: Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany. katja.bettenbrock@mpi-magdeburg.mpg.de.
authors: Katja
authors: K
authors: Bettenbrock