title: Olecular mechanism underlying the myeloperoxidase induced apoptosis of HUVEC-12 cells.
abstract: OBJECTIVE
  This study aimed to investigate the molecular mechanism underlying the myeloperoxidase (MPO) induced apoptosis of human umbilical vein endothelial cells (HUVECs).
  
  
  METHODS
  HUVEC-12 cells were treated with myeloperoxidase at different concentrations (0.1 μ/ml, 0.2 μ/ml, 0.4 μ/ml and 0.6 μ/ml) and apoptotic cells were detected by flow cytometry. Then, cells were harvested for the detection of mRNA and protein expression of caspase-3 and Bax by reverse transcription PCR and Western blot assay, respectively.
  
  
  RESULTS
  When compared with negative control group, the apoptosis index in 0.2 μ/ml MPO group, 0.4 μ/ml MPO group and 0.6 μ/ml MPO group increased markedly (P<0.05). When compared with negative control group, the mRNA expression of caspase-3 in 0.6 μ/ml MPO group and positive control group increased dramatically (P<0.05). When compared with negative control group, the protein expression of pre-caspase-3 and activated caspase-3 elevated significantly in 0.4 μ/ml MPO group, 0.6 μ/ml MPO group and positive control group (P<0.05). When compared with negative control group, the mRNA and protein expression of Bax elevated dramatically in 0.4 μ/ml MPO group, 0.6 μ/ml MPO group and positive control group (P<0.05).
  
  
  CONCLUSIONS
  MPO at certain extents may induce the apoptosis of HUVEC-12. The MPO induced apoptosis of HUVEC-12 may be dependent on capase-3 signaling pathway, and Bax is also involved in the MPO induced apoptosis of HUVEC-12.
authors: Department of Cardiology, The Third Xiangya Hospital of Central South University Changsha, Hunan 410013, China.
authors: Mao
authors: M
authors: Ouyang
authors: Department of Cardiology, The Third Xiangya Hospital of Central South University Changsha, Hunan 410013, China.
authors: Hengdao
authors: H
authors: Liu
authors: Department of Cardiology, The Third Xiangya Hospital of Central South University Changsha, Hunan 410013, China.
authors: Kan
authors: K
authors: Yang
authors: Department of Cardiology, The Third Xiangya Hospital of Central South University Changsha, Hunan 410013, China.
authors: Weihong
authors: W
authors: Jiang
authors: Department of Cardiology, The Third Xiangya Hospital of Central South University Changsha, Hunan 410013, China.
authors: Qi
authors: Q
authors: Ding
authors: Department of Cardiology, The Third Xiangya Hospital of Central South University Changsha, Hunan 410013, China.
authors: Xunzhang
authors: X
authors: Yu
authors: Department of Cardiology, The Third Xiangya Hospital of Central South University Changsha, Hunan 410013, China.
authors: Wu
authors: W
authors: Chen
keywords: Bax; Myeloperoxidase; apoptosis; caspase-3; human umbilical vein endothelial cells