title: Clipping of predetermined fragments from the human genome by S1 nuclease-PNA combinations.
abstract: By combining S1 nuclease with two strands of pseudo-complementary peptide nucleic acid (pcPNA), the whole human genome was selectively cut at targeted sites, and desired fragments were clipped from the genome.
authors: Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8577 Japan. sumaoka@tara.tsukuba.ac.jp komiyama@tara.tsukuba.ac.jp.
authors: Xia
authors: X
authors: Li
authors: Satoshi
authors: S
authors: Muneoka
authors: Narumi
authors: N
authors: Shigi
authors: Jun
authors: J
authors: Sumaoka
authors: Makoto
authors: M
authors: Komiyama
mesh_terms: D000595:Amino Acid Sequence; D002875:Chromosomes / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000737:chemistry*; D016615:Telomere / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D005656:Fungal Proteins; D020135:Peptide Nucleic Acids; D004247:DNA; C526098:Endonuclease S1, Aspergillus; D015719:Single-Strand Specific DNA and RNA Endonucleases