title: Anesthetic agent-specific effects on synaptic inhibition.
abstract: BACKGROUND
  Anesthetics enhance γ-aminobutyric acid (GABA)-mediated inhibition in the central nervous system. Different agents have been shown to act on tonic versus synaptic GABA receptors to different degrees, but it remains unknown whether different forms of synaptic inhibition are also differentially engaged. With this in mind, we tested the hypothesis that different types of GABA-mediated synapses exhibit different anesthetic sensitivities. The present study compared effects produced by isoflurane, halothane, pentobarbital, thiopental, and propofol on paired-pulse GABAA receptor-mediated synaptic inhibition. Effects on glutamate-mediated facilitation were also studied.
  
  
  METHODS
  Synaptic responses were measured in rat hippocampal brain slices. Orthodromic paired-pulse stimulation was used to assess anesthetic effects on either glutamate-mediated excitatory inputs or GABA-mediated inhibitory inputs to CA1 neurons. Antidromic stimulation was used to assess anesthetic effects on CA1 background excitability. Agents were studied at equieffective concentrations for population spike depression to compare their relative degree of effect on synaptic inhibition.
  
  
  RESULTS
  Differing degrees of anesthetic effect on paired-pulse facilitation at excitatory glutamate synapses were evident, and blocking GABA inhibition revealed a previously unseen presynaptic action for pentobarbital. Although all 5 anesthetics depressed synaptically evoked excitation of CA1 neurons, the involvement of enhanced GABA-mediated inhibition differed considerably among agents. Single-pulse inhibition was enhanced by propofol, thiopental, and pentobarbital, but only marginally by halothane and isoflurane. In contrast, isoflurane enhanced paired-pulse inhibition strongly, as did thiopental, but propofol, pentobarbital, and halothane were less effective.
  
  
  CONCLUSIONS
  These observations support the idea that different GABA synapses use receptors with differing subunit compositions and that anesthetics exhibit differing degrees of selectivity for these receptors. The differing anesthetic sensitivities seen in the present study, at glutamate and GABA synapses, help explain the unique behavioral/clinical profiles produced by different classes of anesthetics and indicate that there are selective targets for new agent development.
authors: From the Department of Anesthesia, Stanford School of Medicine, Palo Alto, California.
authors: M Bruce
authors: MB
authors: MacIver
mesh_terms: D000776:Anesthesiology; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D001463:Barbiturates / Q000494:pharmacology; D001640:Bicuculline / Q000494:pharmacology; D056547:CA1 Region, Hippocampal / Q000187:drug effects; D003627:Data Interpretation, Statistical; D003712:Dendrites / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005071:Evoked Potentials / Q000187:drug effects; D018756:GABA Antagonists / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009433:Neural Inhibition / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D019999:Pharmaceutical Solutions; D010852:Picrotoxin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000187:drug effects; D013569:Synapses / Q000187:drug effects*
chemical_list: D000777:Anesthetics; D001463:Barbiturates; D001640:Bicuculline; D018756:GABA Antagonists; D019999:Pharmaceutical Solutions; D010852:Picrotoxin; D011963:Receptors, GABA-A