title: Treatment of MSCs with Wnt1a-conditioned medium activates DP cells and promotes hair follicle regrowth.
abstract: Hair loss (alopecia) is a common problem for people. The dermal papilla is the key signaling center that regulates hair growth and it engage in crosstalk with the microenvironment, including Wnt signaling and stem cells. In this study, we explored the effects of bone marrow mesenchymal stem cell overexpression of Wnt1a on mouse hair follicle regeneration. Wnt-CM accelerated hair follicle progression from telogen to anagen and enhanced the ALP expression in the DP area. Moreover, the hair induction-related genes were upregulated, as demonstrated by qRT-PCR. Wnt-CM treatment restored and increased DP cell expression of genes downregulated by dihydrotestosterone treatment, as demonstrated by qRT-PCR assays. Our study reveals that BM-MSC-generated Wnt1a promotes the DP's ability to induce hair cycling and regeneration.
authors: 1] Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China [2].
authors: Liang
authors: L
authors: Dong
authors: 1] Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China [2].
authors: Haojie
authors: H
authors: Hao
authors: 1] Department of Medical Administration,Chinese PLA General Hospital, Beijing 100853, China [2].
authors: Lei
authors: L
authors: Xia
authors: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors: Jiejie
authors: J
authors: Liu
authors: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors: Dongdong
authors: D
authors: Ti
authors: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors: Chuan
authors: C
authors: Tong
authors: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors: Qian
authors: Q
authors: Hou
authors: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors: Qingwang
authors: Q
authors: Han
authors: Central laboratory, Hainan branch of Chinese PLA General Hospital, Sanya, 572013, China.
authors: Yali
authors: Y
authors: Zhao
authors: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors: Huiling
authors: H
authors: Liu
authors: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors: Xiaobing
authors: X
authors: Fu
authors: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors: Weidong
authors: W
authors: Han
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology*; D020405:Dermis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D057809:HEK293 Cells; D006197:Hair / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018859:Hair Follicle / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051155:Wnt1 Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D051155:Wnt1 Protein