title: RNA interference as a tool for target validation.
abstract: RNA interference (RNAi) is a cellular phenomenon, aspects of which still remain partially unknown. Nevertheless, beyond its function in cellular defense or control of development, RNAi provides a powerful tool for the study of gene function and target validation. Here, we review some of the key technologies that have allowed RNAi to be used 'in vitro' and 'in vivo'. We summarize their main advantages and disadvantages, in the hope of creating a basic guideline for scientists needing to introduce RNAi as a tool for basic research.:
authors: Retrovirology Laboratory IrsiCaixa, Hospital Universitari Germans Trias i Pujol, 08916 Badalona, Spain.
authors: Eduardo
authors: E
authors: Pauls
authors: Retrovirology Laboratory IrsiCaixa, Hospital Universitari Germans Trias i Pujol, 08916 Badalona, Spain. Electronic address:jaeste@ns.hugtip.scs.es.
authors: José A
authors: JA
authors: Esté