title: A model of human nasal epithelial cells adapted for direct and repeated exposure to airborne pollutants.
abstract: Airway epithelium lining the nasal cavity plays a pivotal role in respiratory tract defense and protection mechanisms. Air pollution induces alterations linked to airway diseases such as asthma. Only very few in vitro studies to date have succeeded in reproducing physiological conditions relevant to cellular type and chronic atmospheric pollution exposure. We therefore, set up an in vitro model of human Airway Epithelial Cells of Nasal origin (hAECN) close to real human cell functionality, specifically adapted to study the biological effects of exposure to indoor gaseous pollution at the environmental level. hAECN were exposed under air-liquid interface, one, two, or three-times at 24 h intervals for 1 h, to air or formaldehyde (200 μg/m(3)), an indoor air gaseous pollutant. All experiments were ended at day 4, when both cellular viability and cytokine production were assessed. Optimal adherence and confluence of cells were obtained 96 h after cell seeding onto collagen IV-precoated insert. Direct and repeated exposure to formaldehyde did not produce any cellular damage or IL-6 production change, although weak lower IL-8 production was observed only after the third exposure. Our model is significantly better than previous ones due to cell type and the repeated exposure protocol.
authors: Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France; Agence de l'Environnement et de la Maîtrise de l'Energie, Angers, France. Electronic address: gaelle.bardet@etu.parisdescartes.fr.
authors: Gaëlle
authors: G
authors: Bardet
authors: Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France. Electronic address: sophie.achard@parisdescartes.fr.
authors: Sophie
authors: S
authors: Achard
authors: Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France. Electronic address: t_loret@orange.fr.
authors: Thomas
authors: T
authors: Loret
authors: Centre des Matériaux des Mines d'Alès, Ecole des Mines d'Alès, Pau, France. Electronic address: valerie.desauziers@mines-ales.fr.
authors: Valérie
authors: V
authors: Desauziers
authors: Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France. Electronic address: isabelle.momas@parisdescartes.fr.
authors: Isabelle
authors: I
authors: Momas
authors: Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France; AP-HP, Hôpital Bichat, Biochimie, Paris, France. Electronic address: nathalie.seta@parisdescartes.fr.
authors: Nathalie
authors: N
authors: Seta
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D002460:Cell Line; D003124:Colorimetry; D004396:Coloring Agents; D016207:Cytokines / Q000096:biosynthesis; D003584:Cytological Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects* / Q000648:ultrastructure; D005557:Formaldehyde / Q000633:toxicity; D006801:Humans; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009297:Nasal Mucosa / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D013778:Tetrazolium Salts
chemical_list: D000393:Air Pollutants; D004396:Coloring Agents; D016207:Cytokines; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D005557:Formaldehyde; D007770:L-Lactate Dehydrogenase
keywords: Air–liquid interface; In vitro; Repeated exposures; hAECN (human Airway Epithelial Cells of Nasal origin)