title: Influence of antibodies transferred by colostrum in the immune responses of calves to current foot-and-mouth disease vaccines.
abstract: Immunity to currently used oil-adjuvanted inactivated vaccines against foot-and-mouth disease virus (FMDV) has been studied in detail in adult animals; however, the influence of maternally derived antibodies transferred through colostrum (Mat-Abs) in the immune responses of vaccinated calves is less clear. Here, we report the anti-FMDV humoral responses elicited in calves with or without Mat-Abs that received one or two doses of the current tetravalent oil-adjuvanted commercial vaccine used in Argentina. Anti-FMDV (O1/Campos strain) antibodies (Abs) were evaluated by Liquid Phase Blocking ELISA (LPB-ELISA), virus neutralization test (VNT), isotype ELISA (IgG1, IgG2 and IgM) and avidity ELISA, to allow for the first time a more detailed description of the humoral responses elicited. Our results show that primary IgM responses to FMDV vaccination only became evident as Mat-Abs titers decreased. Likewise, prime and boost vaccination schedules, applied 35 days apart to groups of calves with high or low levels of Mat-Abs, showed that the levels of preexisting neutralizing Mat-Abs prevented the loss of total Abs measured by LPB-ELISA but negatively interfered with the induction of virus neutralizing responses. Altogether, these findings indicate that comprehensive serological characterization of immune responses generated after vaccination in calves may reveal important information on the actual effectiveness of vaccination strategies for young animals, particularly in endemic settings.
authors: INTA, Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Argentina.
authors: Danilo
authors: D
authors: Bucafusco
authors: INTA, Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Buenos Aires, Argentina.
authors: Sebastián
authors: S
authors: Di Giacomo
authors: INTA, Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Argentina.
authors: Juan
authors: J
authors: Pega
authors: Independent Veterinarian.
authors: María Sol
authors: MS
authors: Juncos
authors: INTA, Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Buenos Aires, Argentina.
authors: Juan Manuel
authors: JM
authors: Schammas
authors: INTA, Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Argentina.
authors: Mariano
authors: M
authors: Pérez-Filgueira
authors: INTA, Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Argentina. Electronic address: capozzo.alejandra@inta.gob.ar.
authors: Alejandra Victoria
authors: AV
authors: Capozzo
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D001118:Argentina; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control; D003126:Colostrum / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control; D056724:Immunity, Humoral; D007112:Immunity, Maternally-Acquired; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D009500:Neutralization Tests; D011247:Pregnancy; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014765:Viral Vaccines
keywords: Calves; Colostral immunity; Foot-and-mouth disease vaccines; Maternal antibodies; Serology