title: Carboxymethylation enhances the maturation-inducing activity in dendritic cells of polysaccharide from the seeds of Plantago asiatica L.
abstract: Carboxymethylation is a well-known modification process for polysaccharides. To evaluate the biological availability of carboxymethyl, polysaccharide from the seeds of Plantago asiatica L. (PLCP) was carboxymethylated (CM-PLCP) and the immunomodulatory activities of five CM-PLCPs of gradient degree of substitution (DS) from 0.40 to 0.62 were determined on dendritic cells (DCs) in vitro. Compared with DCs treated with PLCP, DCs treated with CM-PLCP of DS0.50, DS0.55, DS0.62, as well as CD86 and CD80, expressed higher levels of MHCII, CD86 and CD80 surface molecules. In addition, the secretion of IL-12p70 and the mRNA of CCR7 and CXCR4 chemokines were increased, while the endocytosis activities were inhibited. Correspondingly, stronger mixed lymphocyte reactions were induced by the DCs treated with the CM-PLCPs. The results showed that carboxymethylation modification of relevant high DS can enhance the DC maturation-inducing function of PLCP, indicating the potential application of carboxymethylated polysaccharide as an immunotherapeutic adjuvant.
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: Le-Ming
authors: LM
authors: Jiang
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: spnie@ncu.edu.cn.
authors: Shao-Ping
authors: SP
authors: Nie
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: Hua-Lu
authors: HL
authors: Zhou
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: Dan-Fei
authors: DF
authors: Huang
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: myxie@ncu.edu.cn.
authors: Ming-Yong
authors: MY
authors: Xie
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000502:physiology; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D007155:Immunologic Factors / Q000494:pharmacology*; D018664:Interleukin-12 / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D008807:Mice, Inbred BALB C; D010943:Plantago; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D054400:Receptors, CCR7 / Q000235:genetics; D019718:Receptors, CXCR4 / Q000235:genetics; D012639:Seeds
chemical_list: C514936:CXCR4 protein, mouse; C515371:Ccr7 protein, mouse; D007155:Immunologic Factors; D011134:Polysaccharides; D012333:RNA, Messenger; D054400:Receptors, CCR7; D019718:Receptors, CXCR4; D018664:Interleukin-12
keywords: Carboxymethylation; Dendritic cells; Plantago asiatica L.; Polysaccharide