title: Integrated miRNA and mRNA transcriptomes of porcine alveolar macrophages (PAM cells) identifies strain-specific miRNA molecular signatures associated with H-PRRSV and N-PRRSV infection.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is one of the most significant viral diseases in swine, which causes large economic losses to the swine industry worldwide. There is considerable strain variation in PRRSV and two examples of this are the highly virulent Chinese-type PRRSV (H-PRRSV) and the classical North American type PRRSV (N-PRRSV), both with different pathogenesis. These differences may be due in part to genetic and phenotypic differences in virus replication, but also interaction with the host cell. MicroRNAs (miRNAs) are crucial regulators of gene expression and play vital roles in virus and host interactions. However, the regulation role of miRNAs during PRRSV infection has not been systematically investigated. In order to better understand the differential regulation roles of cellular miRNAs in the host response to PRRSV, miRNA expression and a global mRNA transcriptome profile was determined in primary cells infected with either H-PRRSV or N-PRRSV as multiple time points during the viral lifecycle. miRNA-mRNA interactome networks were constructed by integrating the differentially expressed miRNAs and inversely correlated target mRNAs. Using gene ontology and pathway enrichment analyses, cellular pathways associated with deregulated miRNAs were identified, including immune response, phagosome, autophagy, lysosome, autolysis, apoptosis and cell cycle regulation. To our knowledge, this is the first global analysis of strain-specific host miRNA molecular signatures associated with H- and N-PRRSV infection by integrating miRNA and mRNA transcriptomes and provides a new perspective on the contribution of miRNAs to the pathogenesis of PRRSV infection.
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors: Peiqing
authors: P
authors: Cong
authors: Shuqi
authors: S
authors: Xiao
authors: Yaosheng
authors: Y
authors: Chen
authors: Liangliang
authors: L
authors: Wang
authors: Jintao
authors: J
authors: Gao
authors: Ming
authors: M
authors: Li
authors: Zuyong
authors: Z
authors: He
authors: Yunxue
authors: Y
authors: Guo
authors: Guangyin
authors: G
authors: Zhao
authors: Xiaoyu
authors: X
authors: Zhang
authors: Luxi
authors: L
authors: Chen
authors: Delin
authors: D
authors: Mo
authors: Xiaohong
authors: X
authors: Liu
mesh_terms: D000818:Animals; D016676:Macrophages, Alveolar / Q000166:cytology / Q000821:virology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D019318:Porcine Reproductive and Respiratory Syndrome / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA; D013552:Swine; D059467:Transcriptome; D014779:Virus Replication
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger