title: Oligonucleotide-templated reactions based on Peptide Nucleic Acid (PNA) probes: concept and biomedical applications.
abstract: Sensing technologies based on Peptide Nucleic Acids (PNAs) and oligonucleotide-templated chemistry are perfectly suited for biomedical applications (e.g., diagnosis, prognosis and stratification of diseases) and could compete well with more traditional amplification technologies using expensive dual-labelled oligonucleotide probes. PNAs can be easily synthesised and functionalised, are more stable and are more responsive to point-mutations than their DNA counterpart. For these reasons, fluorogenic PNAs represent an interesting alternative to DNA-based molecular beacons for sensing applications in a cell-free environment, where cellular uptake is not required.
authors: Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.
authors: Youngeun
authors: Y
authors: Choi
authors: Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.
authors: Gavin
authors: G
authors: Metcalf
authors: Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.
authors: Mazen Haj
authors: MH
authors: Sleiman
authors: Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.
authors: Douglas
authors: D
authors: Vair-Turnbull
authors: Department of Bioengineering, Imperial College London, London SW7 2AZ, UK. Electronic address: sladame@imperial.ac.uk.
authors: Sylvain
authors: S
authors: Ladame
mesh_terms: D020811:Biomedical Technology; D015342:DNA Probes / Q000737:chemistry; D005453:Fluorescence; D009841:Oligonucleotides / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D009841:Oligonucleotides; D020135:Peptide Nucleic Acids
keywords: Biomarkers; Cancer; Cell-free nucleic acids; Oligonucleotide templated reaction; Peptide Nucleic Acids