title: Wavelength-selective uncaging of oligonucleotides.
abstract: Caged compounds are light-sensitive molecules with temporarily inactivated biological function. The active compound is released upon irradiation, in which exact spatial and temporal control is accomplished. Beyond this inherently irreversible concept of triggering, the idea of multi-wavelength uncaging provides experiments with more complexity. This unit describes the syntheses of protected nucleoside phosphoramidites of caged dT(NpHP) [pHP = (p-hydroxyphenacyl)], dT(DEACM) {DEACM = [(7-diethylaminocoumarin-4-yl)methyl]} or a dC(NDBF) {NDBF = [1-(3-nitrodibenzofuran-1-yl)ethyl]} modification on the nucleobase, their incorporation in oligonucleotides, characterization, and their wavelength-selective uncaging up to four levels.
authors: Institute for Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Goethe-University Frankfurt, Frankfurt, Germany.
authors: Alexandre
authors: A
authors: Rodrigues-Correia
authors: Patrick
authors: P
authors: Seyfried
authors: Alexander
authors: A
authors: Heckel
mesh_terms: D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D055668:Photochemical Processes
chemical_list: D009841:Oligonucleotides
keywords: photolabile-protecting groups; quantum yields; selective photochemistry; uncaging