title: Impact of Hymenoptera venom allergy and the effects of specific venom immunotherapy on mast cell metabolites in sensitized children.
abstract: OBJECTIVE
  Mast cells (MC) are effector cells during severe systemic reactions (SR) to Hymenoptera stings. Venom specific immunotherapy (VIT) is the treatment of choice for prevention of SR to stings. Tryptase and prostaglandin D₂ metabolites (PGD₂) are the markers of MC activation. The study design was to 1. compare baseline values of serum tryptase concentration (BST) and PGD₂ metabolites in children with/without venom sensitization, 2. to evaluate an influence of rush VIT on MC markers in treated children.
  
  
  METHODS
  Sensitized group: 25 children with SR to Hymenoptera sting.
  
  
  METHODS
  19 healthy children. Active treatment: 5-day-rush-VIT. BST was evaluated by ImmunoCAP, PGD₂ metabolites in blood and urine by GC-NICI-MS.
  
  
  RESULTS
  The baseline blood levels of MC markers were significantly higher, while urinary concentration of 9α,11β-PGF₂ was significantly lower in the whole group of venom-sensitized children compared to controls. Severity of SR showed negative correlation with urinary PGD₂ metabolites, while positive with plasma 9α,11β-PGF₂ and BST concentration The highest sensitivity was obtained for plasma 9α,11β-PGF₂ whereas the highest specificity for urinary PGD-M.
  
  
  CONCLUSIONS
  In children with IgE-mediated SR to Hymenoptera stings, elevation of baseline values of PGD₂ metabolites in blood is accompanied by decreased excretion of its urinary metabolites. Assessment of stable PGD₂ metabolites might serve as an independent MC marker to identify allergic children. There is an association between urinary PGD₂ metabolites and severity of the SR to Hymenoptera stings.
authors: Chair of Pediatrics, Department of Pediatrics, Jagiellonian University Medical College, Cracow, Poland.
authors: Ewa
authors: E
authors: Cichocka-Jarosz
authors: Chair of Medicine, Department of Medicine, Jagiellonian University Medical College, Cracow, Poland.
authors: Marek
authors: M
authors: Sanak
authors: Chair of Medicine, Department of Medicine, Jagiellonian University Medical College, Cracow, Poland.
authors: Andrzej
authors: A
authors: Szczeklik
authors: Chair of Epidemiology and Preventive Medicine, Department of Medical Sociology, Jagiellonian University Medical College, Cracow, Poland.
authors: Piotr
authors: P
authors: Brzyski
authors: Chair of Pediatrics, Department of Pediatrics, Jagiellonian University Medical College, Cracow, Poland.
authors: Jacek J
authors: JJ
authors: Pietrzyk
mesh_terms: D000293:Adolescent; D000818:Animals; D001514:Bee Venoms / Q000009:adverse effects / Q000276:immunology / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood / Q000652:urine; D016022:Case-Control Studies; D002648:Child; D003888:Desensitization, Immunologic / Q000009:adverse effects; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006927:Hymenoptera / Q000737:chemistry; D007124:Immunoenzyme Techniques; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D011446:Prospective Studies; D015230:Prostaglandin D2 / Q000097:blood* / Q000652:urine*; D012016:Reference Values; D053802:Tryptases / Q000097:blood*; D014862:Wasp Venoms / Q000009:adverse effects / Q000276:immunology / Q000627:therapeutic use*
chemical_list: D001514:Bee Venoms; D015415:Biomarkers; D014862:Wasp Venoms; D053802:Tryptases; D015230:Prostaglandin D2