title: Rhizobium paranaense sp. nov., an effective N2-fixing symbiont of common bean (Phaseolus vulgaris L.) with broad geographical distribution in Brazil.
abstract: Nitrogen (N), the nutrient most required for plant growth, is key for good yield of agriculturally important crops. Common bean (Phaseolus vulgaris L.) can benefit from bacteria collectively called rhizobia, which are capable of fixing atmospheric nitrogen (N2) in root nodules and supplying it to the plant. Common bean is amongst the most promiscuous legume hosts; several described species, in addition to putative novel ones have been reported as able to nodulate this legume, although not always effectively in terms of fixing N2. In this study, we present data indicating that Brazilian strains PRF 35(T), PRF 54, CPAO 1135 and H 52, currently classified as Rhizobium tropici, represent a novel species symbiont of common bean. Morphological, physiological and biochemical properties differentiate these strains from other species of the genus Rhizobium, as do BOX-PCR profiles (less than 60 % similarity), multilocus sequence analysis with recA, gyrB and rpoA (less than 96.4 % sequence similarity), DNA-DNA hybridization (less than 50 % DNA-DNA relatedness), and average nucleotide identity of whole genomes (less than 92.8.%). The novel species is effective in nodulating and fixing N2 with P. vulgaris, Leucaena leucocephala and Leucaena esculenta. We propose the name Rhizobium paranaense sp. nov. for this novel taxon, with strain PRF 35(T) ( = CNPSo 120(T) = LMG 27577(T) = IPR-Pv 1249(T)) as the type strain.
authors: Universidade Estadual de Londrina, Dept. of Biochemistry and Biotechnology, C.P. 10.011, 86057-970, Londrina, Paraná, Brazil.|Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil.
authors: Rebeca Fuzinatto
authors: RF
authors: Dall'Agnol
authors: Universidade Estadual de Londrina, Dept. of Microbiology, C.P. 10.011, 86057-9970, Londrina, Paraná, Brazil.|Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil.
authors: Renan Augusto
authors: RA
authors: Ribeiro
authors: Universidade Estadual de Londrina, Dept. of Microbiology, C.P. 10.011, 86057-9970, Londrina, Paraná, Brazil.|Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil.
authors: Jakeline Renata Marçon
authors: JRM
authors: Delamuta
authors: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
authors: Ernesto
authors: E
authors: Ormeño-Orrillo
authors: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
authors: Marco Antonio
authors: MA
authors: Rogel
authors: IAPAR, C.P. 481, 86001-970, Londrina, Paraná, Brazil.
authors: Diva Souza
authors: DS
authors: Andrade
authors: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
authors: Esperanza
authors: E
authors: Martínez-Romero
authors: Universidade Estadual de Londrina, Dept. of Microbiology, C.P. 10.011, 86057-9970, Londrina, Paraná, Brazil.|Universidade Estadual de Londrina, Dept. of Biochemistry and Biotechnology, C.P. 10.011, 86057-970, Londrina, Paraná, Brazil.|Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil.
authors: Mariangela
authors: M
authors: Hungria
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D027805:Phaseolus / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D053204:Root Nodules, Plant / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S