title: Hijacking of host cellular functions by an intracellular parasite, the microsporidian Anncaliia algerae.
abstract: Intracellular pathogens including bacteria, viruses and protozoa hijack host cell functions to access nutrients and to bypass cellular defenses and immune responses. These strategies have been acquired through selective pressure and allowed pathogens to reach an appropriate cellular niche for their survival and growth. To get new insights on how parasites hijack host cellular functions, we developed a SILAC (Stable Isotope Labeling by Amino Acids in Cell culture) quantitative proteomics workflow. Our study focused on deciphering the cross-talk in a host-parasite association, involving human foreskin fibroblasts (HFF) and the microsporidia Anncaliia algerae, a fungus related parasite with an obligate intracellular lifestyle and a strong host dependency. The host-parasite cross-talk was analyzed at five post-infection times 1, 6, 12 and 24 hours post-infection (hpi) and 8 days post-infection (dpi). A significant up-regulation of four interferon-induced proteins with tetratricopeptide repeats IFIT1, IFIT2, IFIT3 and MX1 was observed at 8 dpi suggesting a type 1 interferon (IFN) host response. Quantitative alteration of host proteins involved in biological functions such as signaling (STAT1, Ras) and reduction of the translation activity (EIF3) confirmed a host type 1 IFN response. Interestingly, the SILAC approach also allowed the detection of 148 A. algerae proteins during the kinetics of infection. Among these proteins many are involved in parasite proliferation, and an over-representation of putative secreted effectors proteins was observed. Finally our survey also suggests that A. algerae could use a transposable element as a lure strategy to escape the host innate immune system.
authors: Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, Aubière, France.
authors: Johan
authors: J
authors: Panek
authors: Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, Aubière, France.
authors: Hicham
authors: H
authors: El Alaoui
authors: Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, Aubière, France.
authors: Anne
authors: A
authors: Mone
authors: Functional Proteomics Platform. UMR CNRS 5203, Montpellier, France.
authors: Serge
authors: S
authors: Urbach
authors: Functional Proteomics Platform. UMS CNRS 3426, Montpellier, France.
authors: Edith
authors: E
authors: Demettre
authors: Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, Aubière, France.
authors: Catherine
authors: C
authors: Texier
authors: INSERM, UMR1090 TAGC, Marseille, Marseille, France; Aix-Marseille Université, UMR1090 TAGC, Marseille, France; CNRS, Marseille, France.
authors: Christine
authors: C
authors: Brun
authors: INSERM, UMR1090 TAGC, Marseille, Marseille, France; Aix-Marseille Université, UMR1090 TAGC, Marseille, France.
authors: Andreas
authors: A
authors: Zanzoni
authors: Clermont Université, Université d'Auvergne, I.U.T., UFR Pharmacie, Clermont-Ferrand, France; Clermont Université, Université d'Auvergne, EA 4678, Conception, Ingénierie et Développement de l'Aliment et du Médicament, Clermont-Ferrand, France.
authors: Eric
authors: E
authors: Peyretaillade
authors: Clermont Université, Université d'Auvergne, I.U.T., UFR Pharmacie, Clermont-Ferrand, France; Clermont Université, Université d'Auvergne, EA 4678, Conception, Ingénierie et Développement de l'Aliment et du Médicament, Clermont-Ferrand, France.
authors: Nicolas
authors: N
authors: Parisot
authors: Université de Lyon, Université Lyon 1, CNRS, UMR5558, Laboratoire de Biométrie et Biologie Evolutive, Villeurbanne, France.
authors: Emmanuelle
authors: E
authors: Lerat
authors: Clermont Université, Université d'Auvergne, I.U.T., UFR Pharmacie, Clermont-Ferrand, France; Clermont Université, Université d'Auvergne, EA 4678, Conception, Ingénierie et Développement de l'Aliment et du Médicament, Clermont-Ferrand, France.
authors: Pierre
authors: P
authors: Peyret
authors: Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, Aubière, France.
authors: Frederic
authors: F
authors: Delbac
authors: Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, Aubière, France.
authors: David G
authors: DG
authors: Biron
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000469:parasitology; D005656:Fungal Proteins / Q000378:metabolism; D006790:Host-Parasite Interactions; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism / Q000469:parasitology*; D016814:Microsporidia / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020543:Proteome
chemical_list: D004251:DNA Transposable Elements; D005656:Fungal Proteins; D020543:Proteome