title: Pseudoruegeria sabulilitoris sp. nov., isolated from seashore sand.
abstract: A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and ovoid or rod-shaped bacterial strain, GJMS-35(T), was isolated from seashore sand at Geoje Island, South Korea. Strain GJMS-35(T) grew optimally at 28-30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJMS-35(T) clustered with type strains of species of the genus Pseudoruegeria, with which it exhibited 96.97-98.42 % 16S rRNA gene sequence similarity. Sequence similarities to the type strains of other recognized species were less than 96.39 %. Strain GJMS-35(T) contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain GJMS-35(T) were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified glycolipid, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain GJMS-35(T) was 64.1 mol% and its mean DNA-DNA relatedness values with type strains of three species of the genus Pseudoruegeria were 11-21 %. Its differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GJMS-35(T) is set apart from other species of the genus Pseudoruegeria. On the basis of the data presented, strain GJMS-35(T) is considered to represent a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria sabulilitoris sp. nov. is proposed. The type strain is GJMS-35(T) ( = KCTC 42111(T) = NBRC 110380(T)).
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Sooyeon
authors: S
authors: Park
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Yong-Taek
authors: YT
authors: Jung
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Sung-Min
authors: SM
authors: Won
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Jung-Hoon
authors: JH
authors: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012822:Silicon Dioxide; C025204:Ubiquinone Q2