title: Evaluation of total antioxidant status, total oxidant status and oxidative stress index in patients with alopecia areata.
abstract: OBJECTIVE
  In this study, we aimed to evaluate total oxidative stress and total antioxidant capacity in serum samples from patients with Alopesia Areata (AA) in our laboratory conditions.
  
  
  METHODS
  In this study, 46 subjects with AA (26 females, 20 males) and the control subjects of 36 (20 females, 16 males) age- and sex-matched healthy volunteers from our hospital staffs were enrolled (the mean age was 23.7 ± 11.0 years). Blood samples were obtained following an overnight fasting state, and were collected on ice at 4°C. The serum samples were separated from the cells by centrifugation at 3000 rpm for 15 min and were stored at -80°C and used for the analysis of the Total Antioxidant Status (TAS) and Total Oxidant Status (TOS).
  
  
  RESULTS
  Total Antioxidant Status (TAS) and Total Oxidant Status (TOS), Oxidative Stress Index (OSI) (TOS/TAS) levels of AA patients were 1.4777 ± 0.1986; 9.7490 ± 6.0445; 0.6593 ± 0.4069 respectively. TAS; TOS; OSİ (TOS/TAS) levels of controls were 1.4028 ± 0.1687; 9.4627 ± 4.2781; 0.6875 ± 0.3232 respectively. TAS, TOS and OSİ levels showed no significant difference between the control and AA group (p > 0.05).
  
  
  CONCLUSIONS
  Future studies about AA pathogenesis should be based not only on oxidant/antioxidant balance but also on several other factors. Because it was observed that the disease showed recurrence in different situations. Since the selection criteria of patients is affected from disease severity and environmental and genetical factors, multicentric studies with better sampled patient population and higher patient number is required.
authors: Department of Biochemistry, School of Medicine, Mustafa Kemal University 31000 Hatay, Turkey.
authors: Sedat
authors: S
authors: Motor
authors: Department of Biochemistry, School of Medicine, Mustafa Kemal University 31000 Hatay, Turkey.
authors: Sahin
authors: S
authors: Ozturk
authors: Department of Biochemistry, School of Medicine, Mustafa Kemal University 31000 Hatay, Turkey.
authors: Oguzhan
authors: O
authors: Ozcan
authors: Department of Biochemistry, School of Medicine, Mustafa Kemal University 31000 Hatay, Turkey.
authors: Ahmet Burak
authors: AB
authors: Gurpinar
authors: Department of Biochemistry, School of Medicine, Mustafa Kemal University 31000 Hatay, Turkey.
authors: Yesim
authors: Y
authors: Can
authors: Department of Biochemistry, School of Medicine, Mustafa Kemal University 31000 Hatay, Turkey.
authors: Rana
authors: R
authors: Yuksel
authors: Department of Dermatology, School of Medicine, Mustafa Kemal University 31000 Hatay, Turkey.
authors: Julide Zehra
authors: JZ
authors: Yenin
authors: Department of Dermatology, School of Medicine, Mustafa Kemal University 31000 Hatay, Turkey.
authors: Gamze
authors: G
authors: Seraslan
authors: Department of Biochemistry, chool of Medicine, Akdeniz University 07070 Antalya, Turkey.
authors: O Hasan
authors: OH
authors: Ozturk
keywords: Alopecia areata (AA); OSI; TAS; TOS; oxidative stress