title: Creating perfused functional vascular channels using 3D bio-printing technology.
abstract: We developed a methodology using 3D bio-printing technology to create a functional in vitro vascular channel with perfused open lumen using only cells and biological matrices. The fabricated vasculature has a tight, confluent endothelium lining, presenting barrier function for both plasma protein and high-molecular weight dextran molecule. The fluidic vascular channel is capable of supporting the viability of tissue up to 5 mm in distance at 5 million cells/mL density under the physiological flow condition. In static-cultured vascular channels, active angiogenic sprouting from the vessel surface was observed whereas physiological flow strongly suppressed this process. Gene expression analysis was reported in this study to show the potential of this vessel model in vascular biology research. The methods have great potential in vascularized tissue fabrication using 3D bio-printing technology as the vascular channel is simultaneously created while cells and matrix are printed around the channel in desired 3D patterns. It can also serve as a unique experimental tool for investigating fundamental mechanisms of vascular remodeling with extracellular matrix and maturation process under 3D flow condition.
authors: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA; Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA.
authors: Vivian K
authors: VK
authors: Lee
authors: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA; Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA.
authors: Diana Y
authors: DY
authors: Kim
authors: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA; Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA.
authors: Haygan
authors: H
authors: Ngo
authors: Department of Bio and Brain Engineering, KAIST, Daejeon, Republic of Korea.
authors: Young
authors: Y
authors: Lee
authors: Department of Biological Sciences, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA.
authors: Lan
authors: L
authors: Seo
authors: Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors: Seung-Schik
authors: SS
authors: Yoo
authors: Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA.
authors: Peter A
authors: PA
authors: Vincent
authors: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA; Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA. Electronic address: daig@rpi.edu.
authors: Guohao
authors: G
authors: Dai
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D003911:Dextrans / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D018919:Neovascularization, Physiologic; D010477:Perfusion; D066330:Printing, Three-Dimensional; D012313:RNA / Q000737:chemistry; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D003911:Dextrans; D020100:Hydrogels; D012313:RNA
keywords: 3D bio-printing; Hydrogel; Perfused vascularized tissue; Vascular channels