title: Stimuli-sensitive hydrogel based on N-isopropylacrylamide and itaconic acid for entrapment and controlled release of Candida rugosa lipase under mild conditions.
abstract: Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.
authors: Department of Forensics, The Academy of Criminalistic and Police Studies, Cara Dušana 196, 11080 Belgrade, Serbia.
authors: Nikola
authors: N
authors: Milašinović
authors: Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
authors: Zorica
authors: Z
authors: Knežević-Jugović
authors: Department of Organic Chemical Technology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
authors: Nedeljko
authors: N
authors: Milosavljević
authors: Department of Organic Chemical Technology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
authors: Marija
authors: M
authors: Lučić Škorić
authors: Department of Organic Chemical Technology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
authors: Jovanka
authors: J
authors: Filipović
authors: Department of Organic Chemical Technology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
authors: Melina
authors: M
authors: Kalagasidis Krušić
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D002175:Candida / Q000201:enzymology*; D003692:Delayed-Action Preparations; D055119:Elastic Modulus; D004800:Enzymes, Immobilized / Q000494:pharmacology*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000494:pharmacology*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013386:Succinates / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D003692:Delayed-Action Preparations; D004800:Enzymes, Immobilized; D013386:Succinates; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C067295:N-isopropylacrylamide; D008049:Lipase; C005229:itaconic acid