title: Changes in α7β1 integrin signaling after eccentric exercise in heat-shocked rat soleus.
abstract: BACKGROUND
  α7β1 integrin links the extracellular matrix to the focal adhesion (FA) in skeletal muscle and serves as a stabilizing and signal relayer. Heat shock (HS) induces expression of proteins that interact with the FA.
  
  
  METHODS
  Male Wistar rats were assigned to 1 of 3 groups: control (CON); eccentric exercise (EE); or EE+HS (HS). Soleus muscle was analyzed at 2 h and 48 h post-exercise.
  
  
  RESULTS
  The 120-kDa α7 integrin decreased in the EE and HS groups, and the 70-kDa peptide decreased in the EE group at 2 h post-exercise. Total expression of focal adhesion kinase (FAK) and RhoA were decreased in EE and HS at 2 h post-exercise. Expression of phosphorylated FAK(397) decreased in the EE group but not the HS group at 2 h post-exercise.
  
  
  CONCLUSIONS
  Long-duration EE may cause alterations in the FA in rat soleus muscle through the α7 integrin subunit and FAK.
authors: Applied Physiology Laboratory, Department of Health, Sport, and Exercise Sciences, University of Kansas, 101DJ Robinson Center, 1301 Sunnyside Avenue, Lawrence, Kansas, 66045, USA.
authors: Zachary A
authors: ZA
authors: Graham
authors: Chad D
authors: CD
authors: Touchberry
authors: Anisha A
authors: AA
authors: Gupte
authors: Gregory L
authors: GL
authors: Bomhoff
authors: Paige C
authors: PC
authors: Geiger
authors: Philip M
authors: PM
authors: Gallagher
mesh_terms: D000818:Animals; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D018869:Heat-Shock Response; D006358:Hot Temperature; D016023:Integrins / Q000378:metabolism*; D008297:Male; D023421:Models, Animal; D009068:Movement / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D016023:Integrins; D051416:Focal Adhesion Protein-Tyrosine Kinases; C082908:integrin alpha7beta1
keywords: FAK; costamere; eccentric exercise; heat shock; integrin