title: A comparative study of the bacterial community in denitrifying and traditional enhanced biological phosphorus removal processes.
abstract: Denitrifying phosphorus removal is an attractive wastewater treatment process due to its reduced carbon source demand and sludge minimization potential. Two lab-scale sequencing batch reactors (SBRs) were operated in alternating anaerobic-anoxic (A-A) or anaerobic-oxic (A-O) conditions to achieve denitrifying enhanced biological phosphate removal (EBPR) and traditional EBPR. No significant differences were observed in phosphorus removal efficiencies between A-A SBR and A-O SBR, with phosphorus removal rates being 87.9% and 89.0% respectively. The community structures in denitrifying and traditional EBPR processes were evaluated by high-throughput sequencing of the PCR-amplified partial 16S rRNA genes from each sludge. The results obtained showed that the bacterial community was more diverse in A-O sludge than in A-A sludge. Taxonomy and β-diversity analyses indicated that a significant shift occurred in the dominant microbial community in A-A sludge compared with the seed sludge during the whole acclimation phase, while a slight fluctuation was observed in the abundance of the major taxonomies in A-O sludge. One Dechloromonas-related OTU outside the 4 known Candidatus "Accumulibacter" clades was detected as the main OTU in A-A sludge at the stationary operation, while Candidatus "Accumulibacter" dominated in A-O sludge.
authors: Harbin Institute of Technology Shenzhen Graduate School.
authors: Xiao-Mei
authors: XM
authors: Lv
authors: Ming-Fei
authors: MF
authors: Shao
authors: Chao-Lin
authors: CL
authors: Li
authors: Ji
authors: J
authors: Li
authors: Xin-Lei
authors: XL
authors: Gao
authors: Fei-Yun
authors: FY
authors: Sun
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology; D058440:Denitrification; D052918:Environmental Restoration and Remediation / Q000379:methods*; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology
chemical_list: D012722:Sewage; D010758:Phosphorus; D010100:Oxygen