title: Avanti lipid tools: connecting lipids, technology, and cell biology.
abstract: Lipid research is challenging owing to the complexity and diversity of the lipidome. Here we review a set of experimental tools developed for the seasoned lipid researcher, as well as, those who are new to the field of lipid research. Novel tools for probing protein-lipid interactions, applications for lipid binding antibodies, enhanced systems for the cellular delivery of lipids, improved visualization of lipid membranes using gold-labeled lipids, and advances in mass spectrometric analysis techniques will be discussed. Because lipid mediators are known to participate in a host of signal transduction and trafficking pathways within the cell, a comprehensive lipid toolbox that aids the science of lipidomics research is essential to better understand the molecular mechanisms of interactions between cellular components. This article is part of a Special Issue entitled Tools to study lipid functions.
authors: Avanti Polar Lipids, Inc., 700 Industrial Park Drive, Alabaster, Al 35007, USA. Electronic address: kacee.sims@avantilipids.com.
authors: Kacee H
authors: KH
authors: Sims
authors: Avanti Polar Lipids, Inc., 700 Industrial Park Drive, Alabaster, Al 35007, USA. Electronic address: ewan.tytler@avantilipids.com.
authors: Ewan M
authors: EM
authors: Tytler
authors: Avanti Polar Lipids, Inc., 700 Industrial Park Drive, Alabaster, Al 35007, USA. Electronic address: john.tipton@durect.com.
authors: John
authors: J
authors: Tipton
authors: Avanti Polar Lipids, Inc., 700 Industrial Park Drive, Alabaster, Al 35007, USA. Electronic address: kasey.hill@avantilipids.com.
authors: Kasey L
authors: KL
authors: Hill
authors: Avanti Polar Lipids, Inc., 700 Industrial Park Drive, Alabaster, Al 35007, USA. Electronic address: steve.burgess@avantilipids.com.
authors: Stephen W
authors: SW
authors: Burgess
authors: Avanti Polar Lipids, Inc., 700 Industrial Park Drive, Alabaster, Al 35007, USA. Electronic address: walt.shaw@avantilipids.com.
authors: Walter A
authors: WA
authors: Shaw
mesh_terms: D000906:Antibodies / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D006046:Gold / Q000737:chemistry; D008055:Lipids / Q000502:physiology*; D008081:Liposomes; D013058:Mass Spectrometry
chemical_list: D000906:Antibodies; D008055:Lipids; D008081:Liposomes; D006046:Gold
keywords: Gold-conjugated lipids; Lipid toolbox; Lipid-binding antibodies; Lipidomics; Mass spectrometry; Protein–lipid interactions