title: Antioxidant capacity of follicular fluid from patients undergoing in vitro fertilization.
abstract: This study measured the antioxidant activity of follicular fluid (FF) in infertile patients and assessed its possible correlation between ovarian stimulation and pregnancy outcomes. Samples from 191 infertile patients undergoing in vitro fertilization-embryo transfer (IVF-ET) were determined by α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging, reducing power, superoxide radical scavenging, β-Carotene bleaching assay, ferrothiocyanate and thiobarbituric acid assays. The comparison between a positive IVF outcome and FF's antioxidant activity was also studied. The results showed FF had strong antioxidant activity, which equated to common antioxidants Vc and BHT (100 μg/mL). Patients with endometriosis had less efficient antioxidant activity in FF than that of patients with tubal occlusion or polycystic ovary syndrome. In conclusion, this study detected, for the first time, the antioxidant activity of FF from patients undergoing an IVF and the FF exhibited strong antioxidant activity.
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology Wuhan, People's Republic of China.
authors: Bo
authors: B
authors: Huang
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology Wuhan, People's Republic of China.
authors: Zhou
authors: Z
authors: Li
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology Wuhan, People's Republic of China.
authors: Jihui
authors: J
authors: Ai
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology Wuhan, People's Republic of China.
authors: Lixia
authors: L
authors: Zhu
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology Wuhan, People's Republic of China.
authors: Yufeng
authors: Y
authors: Li
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology Wuhan, People's Republic of China.
authors: Lei
authors: L
authors: Jin
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology Wuhan, People's Republic of China.
authors: Hanwang
authors: H
authors: Zhang
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D004624:Embryo Transfer; D004715:Endometriosis / Q000150:complications / Q000378:metabolism; D005184:Fallopian Tube Diseases / Q000150:complications / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry*; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D011085:Polycystic Ovary Syndrome / Q000150:complications / Q000378:metabolism; D011247:Pregnancy; D018873:Pregnancy Rate; D012307:Risk Factors; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D000975:Antioxidants
keywords: Antioxidant activity; endometriosis; follicular fluid; in vitro fertilization