title: Efficient strategy to generate a vectored duck enteritis virus delivering envelope of duck Tembusu virus.
abstract: Duck Tembusu virus (DTMUV) is a recently emerging pathogenic flavivirus that has resulted in a huge economic loss in the duck industry. However, no vaccine is currently available to control this pathogen. Consequently, a practical strategy to construct a vaccine against this pathogen should be determined. In this study, duck enteritis virus (DEV) was examined as a candidate vaccine vector to deliver the envelope (E) of DTMUV. A modified mini-F vector was inserted into the SORF3 and US2 gene junctions of the attenuated DEV vaccine strain C-KCE genome to generate an infectious bacterial artificial chromosome (BAC) of C-KCE (vBAC-C-KCE). The envelope (E) gene of DTMUV was inserted into the C-KCE genome through the mating-assisted genetically integrated cloning (MAGIC) strategy, resulting in the recombinant vector, pBAC-C-KCE-E. A bivalent vaccine C-KCE-E was generated by eliminating the BAC backbone. Immunofluorescence and western blot analysis results indicated that the E proteins were vigorously expressed in C-KCE-E-infected chicken embryo fibroblasts (CEFs). Duck experiments demonstrated that the insertion of the E gene did not alter the protective efficacy of C-KCE. Moreover, C-KCE-E-immunized ducks induced neutralization antibodies against DTMUV. These results demonstrated, for the first time, that recombinant C-KCE-E can serve as a potential bivalent vaccine against DEV and DTMUV.
authors: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultura University, Wuhan 430070, China. zz19841024@126.com.
authors: Zhong
authors: Z
authors: Zou
authors: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultura University, Wuhan 430070, China. 475589992@qq.com.
authors: Zhigang
authors: Z
authors: Liu
authors: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultura University, Wuhan 430070, China. jinmeilin@mail.hzau.edu.cn.
authors: Meilin
authors: M
authors: Jin
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002642:Chick Embryo; D004372:Ducks; D005416:Flavivirus / Q000235:genetics / Q000276:immunology*; D015870:Gene Expression; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics* / Q000276:immunology*; D007114:Immunization; D025922:Mardivirus / Q000235:genetics* / Q000276:immunology*; D011201:Poultry Diseases / Q000401:mortality / Q000517:prevention & control; D064166:Vaccine Potency; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014765:Viral Vaccines