title: Long-term in vivo assessment of bioengineered skin substitutes: a clinical study.
abstract: The aim of the study was an objective in vivo assessment of skin properties after reconstruction with two artificial dermal substitutes, Integra® and Hyalomatrix®. Twenty-seven patients underwent reconstruction of 36 skin-loss sites with full-thickness skin graft, split-thickness skin graft, Hyalomatrix® bioengineered skin substitute and sequential split-thickness skin graft and Integra® bioengineered skin substitute and sequential split-thickness skin graft. Objective assessments were carried out using three instrumental devices: Multi Probe Adapter System MPA; 22 MHz ultrasound skin scan; and Primos Pico for a three-dimensional (3D) skin scan. The skin parameters under study in our sample were: corneometry, transepidermal water loss, elastometry, colorimetry, skin thickness and 3D skin surface pattern. A skin reconstruction with Hyalomatrix seemed to most closely approach the hydration, transepidermal water loss and skin surface 3D pattern of normal skin. A skin reconstruction with Integra seemed to demonstrate the best skin colour feature and elastic properties. Although no statistically significant differences were observed, the descriptive analysis of the outcomes might suggest a better cell regulation, regenerated extracellular matrix and neoangiogenesis with the use of Hyalomatrix, and the formation of a more elastic regenerated dermis, with overall better physical, mechanical and optical properties, with the use of Integra.
authors: Department of Clinical Surgical Diagnostic and Paediatric Sciences, University of Pavia, Italy; Advanced Technologies for Regenerative Medicine and Inductive Surgery Research Centre, University of Pavia, Italy; Plastic and Reconstructive Surgery Unit, Salvatore Maugeri Research and Care Institute, Pavia, Italy.
authors: Giovanni
authors: G
authors: Nicoletti
authors: Federica
authors: F
authors: Brenta
authors: Mariella
authors: M
authors: Bleve
authors: Tommaso
authors: T
authors: Pellegatta
authors: Alberto
authors: A
authors: Malovini
authors: Angela
authors: A
authors: Faga
authors: Paola
authors: P
authors: Perugini
mesh_terms: D001705:Bioprosthesis; D004548:Elasticity; D005260:Female; D005500:Follow-Up Studies; D006085:Graft Survival; D006801:Humans; D008297:Male; D017768:Skin, Artificial
keywords: bioengineered skin; dermal substitute; skin graft; skin reconstruction; wound repair