title: Probing the binding of an endocrine disrupting compound-Bisphenol F to human serum albumin: insights into the interactions of harmful chemicals with functional biomacromolecules.
abstract: Bisphenol F (BPF) as an endocrine disrupting compounds (EDCs) pollutant in the environment poses a great threat to human health. To evaluate the toxicity of BPF at the protein level, the effects of BPF on human serum albumin (HSA) were investigated at three temperatures 283, 298, and 308 K by multiple spectroscopic techniques. The experimental results showed that BPF effectively quenched the intrinsic fluorescence of HSA via static quenching. The number of binding sites, the binding constant, the thermodynamic parameters and the binding subdomain were measured, and indicated that BPF could spontaneously bind with HSA on subdomain IIA through H-bond and van der Waals interactions. Furthermore, the conformation of HSA was demonstrably changed in the presence of BPF. The work provides accurate and full basic data for clarifying the binding mechanisms of BPF with HSA in vivo and is helpful for understanding its effect on protein function during its transportation and distribution in blood.
authors: College of Chemistry, Liaoning University, 66 Chongshan Middle Road, Shenyang 110036, People's Republic of China.
authors: Fang
authors: F
authors: Pan
authors: Liaoning Environmental Monitoring & Experiment Center, 30A-3 Shuangyuan Road, Shenyang 110161, People's Republic of China.
authors: Tianci
authors: T
authors: Xu
authors: College of Chemistry, Liaoning University, 66 Chongshan Middle Road, Shenyang 110036, People's Republic of China.
authors: Lijun
authors: L
authors: Yang
authors: College of Chemistry, Liaoning University, 66 Chongshan Middle Road, Shenyang 110036, People's Republic of China.
authors: Xiaoqing
authors: X
authors: Jiang
authors: College of Chemistry, Liaoning University, 66 Chongshan Middle Road, Shenyang 110036, People's Republic of China. Electronic address: zhanglei63@126.com.
authors: Lei
authors: L
authors: Zhang
mesh_terms: D001559:Benzhydryl Compounds / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002942:Circular Dichroism; D052244:Endocrine Disruptors / Q000737:chemistry / Q000378:metabolism*; D004735:Energy Transfer; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances / Q000378:metabolism*; D008968:Molecular Conformation; D015335:Molecular Probes / Q000378:metabolism; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D000098833:Bisphenol F Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D046911:Macromolecular Substances; D015335:Molecular Probes; D012709:Serum Albumin; C008745:4,4'-bisphenol F; D000098833:Bisphenol F Compounds
keywords: Bisphenol F; Human serum albumin; Interaction; Multi-spectroscopic techniques