title: Modified PEHPS medium as an alternative for the in vitro culture of Giardia lamblia.
abstract: Commercial culture media present interlot variations in biological activity. We have previously designed a homemade and economic culture medium, PEHPS medium, for the axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. Trophozoites of amoebae and trichomonads grow well in this medium. Furthermore, the medium is stable for several months when stored frozen or refrigerated. The objective of this work was to modify PEHPS medium to support the in vitro growth of Giardia lamblia. Inocula of 5 × 10(3) trophozoites/mL of G. lamblia were incubated at 36.5°C in modified PEHPS or TYI-S-33 medium. Then, the growths of the three Giardia strains in both media were compared. The logarithmic growth phase lasted 72 h; the mean yield of the strains ranged from 10.06 to 11.43 × 10(5) Giardia trophozoites/mL, and the range of duplication time in the three strains was from 5.67 to 6.06 in modified PEHPS medium. These growth characteristics were not significantly different from those obtained with TYI-S-33 medium. We conclude that modified PEHPS medium might be used for the axenic cultivation of G. lamblia.
authors: Laboratorio de Bioquímica y Fisiología Celular, División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano Del Seguro Social, Administración de Correo No. 4, Apartado Postal 020-E Colonia Independencia, 64720 Monterrey, NL, Mexico.
authors: Javier
authors: J
authors: Vargas-Villarreal
authors: Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Avenida Universidad s/n, Ciudad Universitaria, 66451 San Nicolás de los Garza, NL, Mexico.
authors: Benito D
authors: BD
authors: Mata-Cárdenas
authors: Laboratorio de Bioquímica y Fisiología Celular, División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano Del Seguro Social, Administración de Correo No. 4, Apartado Postal 020-E Colonia Independencia, 64720 Monterrey, NL, Mexico ; Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Avenida Pedro de Alba y Manuel L. Barragán s/n, Ciudad Universitaria, 66451 San Nicolás de los Garza, NL, Mexico.
authors: Magda E
authors: ME
authors: Hernández-García
authors: Laboratorio de Bioquímica y Fisiología Celular, División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano Del Seguro Social, Administración de Correo No. 4, Apartado Postal 020-E Colonia Independencia, 64720 Monterrey, NL, Mexico.
authors: Jesús N
authors: JN
authors: Garza-González
authors: Coordinador Auxiliar Medico, Instituto Mexicano Del Seguro Social, Delegación Nuevo León, Gregorio Torres de Quevedo No. 1950 Ote, 64000 Monterrey, NL, Mexico.
authors: Laura H
authors: LH
authors: De La Garza-Salinas
authors: Laboratorio de Bioquímica y Fisiología Celular, División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano Del Seguro Social, Administración de Correo No. 4, Apartado Postal 020-E Colonia Independencia, 64720 Monterrey, NL, Mexico.
authors: Francisco
authors: F
authors: González-Salazar
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology*; D016829:Giardia lamblia / Q000187:drug effects / Q000254:growth & development*; D013997:Time Factors; D053058:Trophozoites / Q000187:drug effects / Q000254:growth & development
chemical_list: D003470:Culture Media