title: Cross-validation of serial optical coherence scanning and diffusion tensor imaging: a study on neural fiber maps in human medulla oblongata.
abstract: We established a strategy to perform cross-validation of serial optical coherence scanner imaging (SOCS) and diffusion tensor imaging (DTI) on a postmortem human medulla. Following DTI, the sample was serially scanned by SOCS, which integrates a vibratome slicer and a multi-contrast optical coherence tomography rig for large-scale three-dimensional imaging at microscopic resolution. The DTI dataset was registered to the SOCS space. An average correlation coefficient of 0.9 was found between the co-registered fiber maps constructed by fractional anisotropy and retardance contrasts. Pixelwise comparison of fiber orientations demonstrated good agreement between the DTI and SOCS measures. Details of the comparison were studied in regions exhibiting a variety of fiber organizations. DTI estimated the preferential orientation of small fiber tracts; however, it didn't capture their complex patterns as SOCS did. In terms of resolution and imaging depth, SOCS and DTI complement each other, and open new avenues for cross-modality investigations of the brain.
authors: Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
authors: Hui
authors: H
authors: Wang
authors: Department of Industrial and Systems Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
authors: Junfeng
authors: J
authors: Zhu
authors: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA; Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors: Martin
authors: M
authors: Reuter
authors: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
authors: Louis N
authors: LN
authors: Vinke
authors: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
authors: Anastasia
authors: A
authors: Yendiki
authors: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
authors: David A
authors: DA
authors: Boas
authors: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA; Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors: Bruce
authors: B
authors: Fischl
authors: Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: akkin@umn.edu.
authors: Taner
authors: T
authors: Akkin
mesh_terms: D056324:Diffusion Tensor Imaging / Q000379:methods / Q000592:standards*; D006801:Humans; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology*; D008875:Middle Aged; D064847:Multimodal Imaging / Q000379:methods / Q000592:standards; D009413:Nerve Fibers, Myelinated / Q000473:pathology; D041623:Tomography, Optical Coherence / Q000379:methods / Q000592:standards*
keywords: Anisotropy; Connectome; Diffusion magnetic resonance imaging; Fiber orientation; Human brain; Optical coherence tomography; Polarization; Validation