title: Determination of inflammatory biomarkers by immunoaffinity capillary electrophoresis.
abstract: Advances in instrumentation and methodologies are urgently needed to achieve, rapid, simultaneous and sensitive determination of multiple substances found at a wide range of concentrations in biological fluids, tissues and cells. The application of immunoaffinity capillary electrophoresis in life sciences is already having an impact on the quantification of many biomarkers for diagnosis and monitoring the prognosis of diseases. This review explains how immunoaffinity capillary electrophoresis, the combination of highly selective antibody capture agents with the high resolving power of capillary electrophoresis, can provide highly specific assays leading to the selective isolation, concentration, separation and quantification of analytes of interest in complex biological matrices. In addition to a discussion of the technology, some applications of clinical and pharmaceutical relevance will be presented.:
authors: Bioanalytical Drug Metabolism, Johnson & Johnson Pharmaceutical, Research & Development, L.L.C., 1000 Route 202, P.O. Box 300, Raritan, NJ 08869, USA. Electronic address:nguzman@prdus.jnj.com.
authors: Norberto A
authors: NA
authors: Guzman
authors: Global Bioanalytical, GPCD, Johnson & Johnson Pharmaceutical, Research & Development, L.L.C., Welsh & McKean Roads, Spring House, PA 19477, USA.
authors: R John
authors: RJ
authors: Stubbs
authors: Ultramicro Analytical Immunochemistry, Division of Bioengineering and Physical Sciences, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA.
authors: Terry M
authors: TM
authors: Phillips