title: Autologous protein solution prepared from the blood of osteoarthritic patients contains an enhanced profile of anti-inflammatory cytokines and anabolic growth factors.
abstract: The objective of this clinical study was to test if blood from osteoarthritis (OA) patients (n = 105) could be processed by a device system to form an autologous protein solution (APS) with preferentially increased concentrations of anti-inflammatory cytokines compared to inflammatory cytokines. To address this objective, APS was prepared from patients exhibiting radiographic evidence of knee OA. Patient metrics were collected including: demographic information, medical history, medication records, and Knee Injury and Osteoarthritis Outcome Score (KOOS) surveys. Cytokine and growth factor concentrations in whole blood and APS were measured using enzyme-linked immunosorbent assays. Statistical analyses were used to identify relationships between OA patient metrics and cytokines. The results of this study indicated that anti-inflammatory cytokines were preferentially increased compared to inflammatory cytokines in APS from 98% of OA patients. APS contained high concentrations of anti-inflammatory proteins including 39,000 ± 20,000 pg/ml IL-1ra, 21,000 ± 5,000 pg/ml sIL-1RII, 2,100 ± 570 pg/ml sTNF-RI, and 4,200 ± 1,500 pg/ml sTNF-RII. Analysis of the 82 patient metrics indicated that no single patient metric was strongly correlated (R(2)  > 0.7) with the key cytokine concentrations in APS. Therefore, APS can be prepared from a broad range of OA patients.
authors: Biomet Biologics, Warsaw, Indiana.
authors: Krista
authors: K
authors: O'Shaughnessey
authors: Andrea
authors: A
authors: Matuska
authors: Jacy
authors: J
authors: Hoeppner
authors: Jack
authors: J
authors: Farr
authors: Mark
authors: M
authors: Klaassen
authors: Christopher
authors: C
authors: Kaeding
authors: Christian
authors: C
authors: Lattermann
authors: William
authors: W
authors: King
authors: Jennifer
authors: J
authors: Woodell-May
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001804:Blood Transfusion, Autologous; D016022:Case-Control Studies; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood* / Q000502:physiology; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000097:blood* / Q000473:pathology / Q000628:therapy; D015854:Up-Regulation / Q000502:physiology*; D055815:Young Adult
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D036341:Intercellular Signaling Peptides and Proteins
keywords: APS; IL-1; IRAP; TNFα; pain; platelet-rich plasma