title: Anti-inflammatory effects of polihexanide and polyethylene glycol in an in vitro study in chronic rhinosinusitis.
abstract: The majority of patients affected by chronic rhinosinusitis (CRS) suffer from eosinophilic infiltration. We hypothesised that polihexanide and polyethylene glycol as an antiseptic might alter the eosinophil-associated IL-5 and eotaxin-3 expression in CRS and also the expression of MMP-9, being involved in the tissue-remodelling in CRS. After obtaining samples from 10 CRS patients with (CRSwNP) and without nasal-polyposis (CRSsNP) and 2 patients with inverted-papilloma undergoing functional endoscopic sinus surgery (FESS), the expression of interleukin-5, eotaxin-3 and MMP-9 were evaluated by an ELISA assay with and without the tested agent. CRSwNP showed a significantly increased expression of IL-5. Polihexanide seems not to alter the attraction of eosinophils in patients with CRS via IL-5 expression. Also elevated levels of MMP-9 could not be reduced to normal values. But there exists statistically significant evidence that the self-amplification process of eosinophils via eotaxin-3 could be influenced by the administration of polihexanide.
authors: Department of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany richard.birk@medma.uni-heidelberg.de.
authors: Richard
authors: R
authors: Birk
authors: Department of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors: Judith
authors: J
authors: Grossbaier
authors: Department of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors: Christoph
authors: C
authors: Aderhold
authors: Department of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors: Jens
authors: J
authors: Stern-Sträter
authors: Department of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors: Karl
authors: K
authors: Hörmann
authors: Department of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors: Alexander
authors: A
authors: Sauter
authors: Department of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors: J Ulrich
authors: JU
authors: Sommer
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001645:Biguanides / Q000494:pharmacology*; D002478:Cells, Cultured; D000075906:Chemokine CCL26; D019742:Chemokines, CC / Q000378:metabolism; D002908:Chronic Disease; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015848:Interleukin-5 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009297:Nasal Mucosa / Q000187:drug effects / Q000378:metabolism; D009298:Nasal Polyps / Q000150:complications; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012220:Rhinitis / Q000150:complications / Q000378:metabolism*; D012852:Sinusitis / Q000150:complications / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D001645:Biguanides; C120429:CCL26 protein, human; D000075906:Chemokine CCL26; D019742:Chemokines, CC; D015848:Interleukin-5; C031233:polihexanide; D011092:Polyethylene Glycols; D020780:Matrix Metalloproteinase 9
keywords: Chronic rhinosinusitis; IL-5; MMP-9; anti-inflammatory; eotaxin-3; polihexanide; polyethylene glycol