title: Automated touch screen device for recording complex rodent behaviors.
abstract: BACKGROUND
  Monitoring mouse behavior is a critical step in the development of modern pharmacotherapies.
  
  
  METHODS
  Here we describe the application of a novel method that utilizes a touch display computer (tablet) and software to detect, record, and report fine motor behaviors. A consumer-grade tablet device is placed in the bottom of a specially made acrylic cage allowing the animal to walk on the device (MouseTrapp). We describe its application in open field (for general locomotor studies) which measures step lengths and velocity. The device can perform light-dark (anxiety) tests by illuminating half of the screen and keeping the other half darkened. A divider is built into the lid of the device allowing the animal free access to either side.
  
  
  RESULTS
  Treating mice with amphetamine and the delta opioid peptide receptor agonist SNC80 stimulated locomotor activity on the device. Amphetamine increased step velocity but not step length during its peak effect (40-70min after treatment), thus indicating detection of subtle amphetamine-induced effects. Animals showed a preference (74% of time spent) for the darkened half compared to the illuminated side.
  
  
  CONCLUSIONS
  Animals were videotaped within the chamber to compare quadrant crosses to detect motion on the device. The slope, duration and magnitude of quadrant crosses tightly correlated with overall locomotor activity as detected by MouseTrapp.
  
  
  CONCLUSIONS
  We suggest that modern touch display devices such as MouseTrapp will be an important step toward automation of behavioral analyses for characterizing phenotypes and drug effects.
authors: Neurolytical LLC, Ann Arbor, MI, United States; Department of Pharmacology, Ann Arbor, MI, United States; Department of Chemistry, University of Michigan, Ann Arbor, MI, United States.
authors: O S
authors: OS
authors: Mabrouk
authors: Department of Pharmacology, Ann Arbor, MI, United States.
authors: I J
authors: IJ
authors: Dripps
authors: Department of Pharmacology, Ann Arbor, MI, United States.
authors: S
authors: S
authors: Ramani
authors: Department of Pharmacology, Ann Arbor, MI, United States.
authors: C
authors: C
authors: Chang
authors: Department of Chemistry, University of Michigan, Ann Arbor, MI, United States.
authors: J L
authors: JL
authors: Han
authors: Chemical Biology Research Branch, National Institute on Drug Abuse and National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD, United States.
authors: K C
authors: KC
authors: Rice
authors: Department of Pharmacology, Ann Arbor, MI, United States. Electronic address: ejutkiew@umich.edu.
authors: E M
authors: EM
authors: Jutkiewicz
mesh_terms: D056044:Actigraphy / Q000295:instrumentation / Q000379:methods; D000661:Amphetamine / Q000494:pharmacology; D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D001007:Anxiety / Q000175:diagnosis / Q000503:physiopathology; D001522:Behavior, Animal; D001549:Benzamides / Q000494:pharmacology; D001696:Biomechanical Phenomena; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D005260:Female; D008297:Male; D008810:Mice, Inbred C57BL; D008838:Microcomputers; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D010363:Pattern Recognition, Automated / Q000379:methods*; D010879:Piperazines / Q000494:pharmacology; D017465:Receptors, Opioid, delta / Q000819:agonists / Q000378:metabolism; D012984:Software; D014110:Touch; D014584:User-Computer Interface
chemical_list: D000701:Analgesics, Opioid; D001549:Benzamides; D000697:Central Nervous System Stimulants; D010879:Piperazines; D017465:Receptors, Opioid, delta; C088464:4-(alpha-(4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl)-N,N-diethylbenzamide; D000661:Amphetamine
keywords: Anxiety; Behavior; Light–dark box; Motor function; Mouse; Movement; Open field; Technology