title: RNA-Seq-based transcriptome analysis of methicillin-resistant Staphylococcus aureus biofilm inhibition by ursolic acid and resveratrol.
abstract: Bacterial biofilms are particularly problematic since they become resistant to most available antibiotics. Hence, novel potential antagonists to inhibit biofilm formation are urgent. Here the influences of two natural products, ursolic acid and resveratrol, on biofilm of the clinical methicillin-resistant Staphylococcus aureus (MRSA) isolate were investigated using RNA-seq, and the differentially expressed genes were analyzed using Cuffdiff. The results showed that ursolic acid inhibition of biofilm formation may reduce amino acids metabolism and adhesins expression and resveratrol may disturb quorum sensing (QS) and the synthesis of surface proteins and capsular polysaccharides. In addition, the transcriptome analysis of resveratrol and the combination of resveratrol with vancomycin inhibition of established biofilm revealed that resveratrol would disturb the expression of genes related to QS, surface and secreted proteins, and capsular polysaccharides. These findings suggest that ursolic acid and resveratrol could be useful to be adjunct therapies for the treatment of MRSA biofilm-involved infections.
authors: 1] State Key Laboratory for Diagnosis and Treatment of Infectious Disease, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China [2].
authors: Nan
authors: N
authors: Qin
authors: 1] School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China [2].
authors: Xiaojuan
authors: X
authors: Tan
authors: 1] Department of Bioinformatics, State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China [2].
authors: Yinming
authors: Y
authors: Jiao
authors: 1] State Key Laboratory for Diagnosis and Treatment of Infectious Disease, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China [2] NGS Sequencing Department, Beijing Genomics Institute (BGI), Shenzhen 518083, China.
authors: Lin
authors: L
authors: Liu
authors: Clinical Laboratory Department of the First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors: Wangsheng
authors: W
authors: Zhao
authors: NGS Sequencing Department, Beijing Genomics Institute (BGI), Shenzhen 518083, China.
authors: Shuang
authors: S
authors: Yang
authors: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors: Aiqun
authors: A
authors: Jia
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D015415:Biomarkers / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000235:genetics* / Q000382:microbiology; D013267:Stilbenes / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*; D000097245:Ursolic Acid
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D001426:Bacterial Proteins; D015415:Biomarkers; D012329:RNA, Bacterial; D012333:RNA, Messenger; D000077185:Resveratrol; D013267:Stilbenes; D014315:Triterpenes; D000097245:Ursolic Acid