title: Evaluation of gene expression changes of serotonin receptors, 5-HT3AR and 5-HT2AR as main stress factors in breast cancer patients.
abstract: Breast cancer is a serious and potentially lethal multi-factor disease among 40-50 aged women in both developed and developing countries. Also, various studies have pointed to roles of neurotransmitters like serotonin in development of cancers, through action on various types of receptors. This study was conducted to evaluate serotonin receptor (5HT2AR and 5HT3AR) genes expression in peripheral blood mononuclear cells (PBMCs) of breast cancer patients in comparison with the healthy people and in the MCF7 cell line. Peripheral blood samples were obtained from 30 patients and 30 healthy individuals. Total RNA was extracted from PBMCs and MCF-7 cells. and 5HT2AR and 5HT3AR were detected by RT-PCR techniques. Finally, serotonin receptor gene expression variation in breast cancer patients and MCF-7 cells were determined by real time-PCR. This latter indicated significant promotion in expression of 5HT3AR and 5HT2AR in PBMCs in breast cancer patients but expression of 5HT2AR in the MCF-7 cell line was significantly decreased. In conclusion, after performing complimentary tests, determine of gene expression changes in serotonin receptors (5HT2AR and 5HT3AR) may be useful as a new approach in treatment of breast cancer based on use of antagonists.
authors: Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran E-mail : ghah@nigeb.ac.ir.
authors: Seyed Hesam
authors: SH
authors: Hejazi
authors: Ghasem
authors: G
authors: Ahangari
authors: Majid
authors: M
authors: Pornour
authors: Abdolkhaleagh
authors: A
authors: Deezagi
authors: Saeed
authors: S
authors: Aminzadeh
authors: Hamid Reza
authors: HR
authors: Ahmadkhaniha
authors: Mohamad Esmail
authors: ME
authors: Akbari
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D061986:MCF-7 Cells; D044402:Receptor, Serotonin, 5-HT2A / Q000235:genetics*; D044406:Receptors, Serotonin, 5-HT3 / Q000235:genetics*
chemical_list: D044402:Receptor, Serotonin, 5-HT2A; D044406:Receptors, Serotonin, 5-HT3