title: The WWOX gene inhibits the growth of U266 multiple myeloma cells by triggering the intrinsic apoptotic pathway.
abstract: The role of the WW domain-containing oxidoreductase (WWOX) gene in multiple types of solid human cancers has been documented extensively. However, the functional role of WWOX in human multiple myeloma has not yet been fully elucidated. The present study aimed to investigate the effects of exogenous WWOX expression on the biological properties of U266 multiple myeloma cells, as well as the possible molecular mechanisms involved. In vitro experiments revealed that exogenous WWOX cDNA transfection resulted in marked growth arrest and the induction of apoptosis in the U266 multiple myeloma cells, accompanied by the activation of the intrinsic apoptotic pathway. Our data provide evidence that WWOX also plays a role as a tumor suppressor gene in multiple myeloma, possibly by suppressing cell proliferation and promoting apoptosis by triggering the intrinsic apoptotic pathway.
authors: Department of Pathology, Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, P.R. China.
authors: Hongsheng
authors: H
authors: Zhang
authors: Department of Pathology, Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, P.R. China.
authors: Lingying
authors: L
authors: Kong
authors: Department of Pathology, Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, P.R. China.
authors: Zhaolei
authors: Z
authors: Cui
authors: Department of Pathology, Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, P.R. China.
authors: Wei
authors: W
authors: Du
authors: Department of Pathology, Fujian Provincial Hospital, Fuzhou, Fujian 350001, P.R. China.
authors: Yihui
authors: Y
authors: He
authors: Department of Pathology, Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, P.R. China.
authors: Zhi
authors: Z
authors: Yang
authors: Department of Pathology, Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, P.R. China.
authors: Li
authors: L
authors: Wang
authors: Department of Pathology, Fujian Provincial Hospital, Fuzhou, Fujian 350001, P.R. China.
authors: Xiaoyan
authors: X
authors: Chen
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology*; D010088:Oxidoreductases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014162:Transfection; D014410:Tumor Stem Cell Assay; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D000074584:WW Domain-Containing Oxidoreductase
chemical_list: D025521:Tumor Suppressor Proteins; D049452:Green Fluorescent Proteins; D010088:Oxidoreductases; D000074584:WW Domain-Containing Oxidoreductase; C486071:WWOX protein, human