title: Association study of childhood obesity with eight genetic variants recently identified by genome-wide association studies.
abstract: BACKGROUND
  Being overweight or obese is becoming increasingly common in low- and middle-income countries. The present study aimed to examine association of eight genetic variants with obesity and to estimate the cumulative effects of these variants in Chinese children.
  
  
  METHODS
  We conducted the case-control study in a total of 2,030 subjects. Genotyping of seven novel variants was performed with matrix-assisted laser desorption ionization time of flight mass spectrometry, while rs9939609 was genotyped with tetra-primer amplification refractory mutation system analysis.
  
  
  RESULTS
  The association of two fat mass and obesity-associated gene (FTO) single-nucleotide polymorphisms (SNPs; rs9939609 and rs62048402) with body mass index (BMI) or obesity reached nominal significance at P < 0.05. We found a cumulative effect of five SNPs on the risk of overweight and obesity (odds ratio (OR) = 1.197, 95% confidence interval (CI) = 1.068-1.342, P = 0.002). Subjects carrying 9 or more effect alleles had a 127% increased risk of overweight and obesity (OR = 2.270, 95% CI = 1.403-3.671, P = 0.001) compared with subjects who carry 6 or fewer effect alleles.
  
  
  CONCLUSIONS
  We confirmed two FTO SNPs (rs62048402 and rs9939609) had nominal significant effects on BMI or obesity. We identified the cumulative effect of five SNPs on risk of overweight and obesity. The results provided evidence for identifying genetic factors related to childhood obesity.
authors: Institute of Child and Adolescent Health, School of Public Health, Peking University, Beijing, China.
authors: Xiang-Rui
authors: XR
authors: Meng
authors: Institute of Child and Adolescent Health, School of Public Health, Peking University, Beijing, China.
authors: Jie-Yun
authors: JY
authors: Song
authors: Institute of Child and Adolescent Health, School of Public Health, Peking University, Beijing, China.
authors: Jun
authors: J
authors: Ma
authors: Institute of Child and Adolescent Health, School of Public Health, Peking University, Beijing, China.
authors: Fang-Hong
authors: FH
authors: Liu
authors: Institute of Child and Adolescent Health, School of Public Health, Peking University, Beijing, China.
authors: Xiao-Rui
authors: XR
authors: Shang
authors: Institute of Child and Adolescent Health, School of Public Health, Peking University, Beijing, China.
authors: Xu-Jun
authors: XJ
authors: Guo
authors: Institute of Child and Adolescent Health, School of Public Health, Peking University, Beijing, China.
authors: Hai-Jun
authors: HJ
authors: Wang
mesh_terms: D050154:Adiposity / Q000208:ethnology / Q000235:genetics; D000293:Adolescent; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; D044466:Asian People / Q000235:genetics; D015992:Body Mass Index; D016022:Case-Control Studies; D002648:Child; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D006801:Humans; D007438:Introns; D016015:Logistic Models; D008297:Male; D016017:Odds Ratio; D063766:Pediatric Obesity / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011506:Proteins / Q000235:genetics*; D012307:Risk Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011506:Proteins; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C526492:FTO protein, human