title: Immune cell imaging using multi-spectral optoacoustic tomography.
abstract: Multispectral optoacoustic tomography (MSOT) offers the potential to image in high-resolution cells tagged with optical labels. In contrast to single wavelength imaging, multispectral excitation and spectral unmixing can differentiate labeled moieties over tissue absorption in the absence of background measurements. This feature can enable longitudinal cellular biology studies well beyond the depths reached by optical microscopy. However, the relation between spectrally resolved fluorescently labeled cells and optoacoustic detection has not been systematically investigated. Herein, we measured titrations of fluorescently labeled cells and establish the optoacoustic signal generated by these cells as a function of cell number and across different cell types. We then assess the MSOT sensitivity to resolve cells implanted in animals.
authors: Stratis
authors: S
authors: Tzoumas
authors: Angelika
authors: A
authors: Zaremba
authors: Uwe
authors: U
authors: Klemm
authors: Antonio
authors: A
authors: Nunes
authors: Karin
authors: K
authors: Schaefer
authors: Vasilis
authors: V
authors: Ntziachristos
mesh_terms: D000818:Animals; D002232:Carbocyanines; D002460:Cell Line; D005456:Fluorescent Dyes; D006801:Humans; D007107:Immune System / Q000166:cytology*; D019169:Jurkat Cells; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000637:transplantation; D051379:Mice; D055620:Optical Phenomena; D019047:Phantoms, Imaging; D061088:Photoacoustic Techniques / Q000379:methods*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D041622:Tomography, Optical / Q000379:methods*
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C024286:3,3'-dioctadecylindocarbocyanine