title: Mycoparasitism studies of Trichoderma harzianum against Sclerotinia sclerotiorum: evaluation of antagonism and expression of cell wall-degrading enzymes genes.
abstract: Trichoderma spp. are known for their biocontrol activity against several plant pathogens. A specific isolate of Trichoderma harzianum, 303/02, has the potential to inhibit the growth of Sclerotinia sclerotiorum, an important agent involved in several crop diseases. In this study, the interaction between T. harzianum 303/02 and mycelia, sclerotia and apothecia of S. sclerotiorum was studied by scanning electron microscopy. RT-qPCR was used to examine the expression of 11 genes potentially involved in biocontrol. T. harzianum 303/02 parasitizes S. sclerotiorum by forming branches that coil around the hyphae. The fungus multiplied abundantly at the sclerotia and apothecia surface, forming a dense mycelium that penetrated the inner surface of these structures. The levels of gene expression varied according to the type of structure with which T. harzianum was interacting. The data also showed the presence of synergistic action between the cell-wall degrading enzymes.
authors: Department of Biochemistry and Molecular Biology, Federal University of Goiás (ICB), Goiânia, GO, 74001-970, Brazil.
authors: Rogério Fraga
authors: RF
authors: Troian
authors: Andrei Stecca
authors: AS
authors: Steindorff
authors: Marcelo Henrique Soller
authors: MH
authors: Ramada
authors: Walquiria
authors: W
authors: Arruda
authors: Cirano José
authors: CJ
authors: Ulhoa
mesh_terms: D001203:Ascomycota / Q000254:growth & development*; D002473:Cell Wall / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008855:Microscopy, Electron, Scanning; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
chemical_list: D005656:Fungal Proteins