title: Determination of saponins and flavonoids in ivy leaf extracts using HPLC-DAD.
abstract: A new method for the determination of six compounds, chlorogenic acid, rutin, nicotiflorin, hederacoside C, hederasaponin B and α-hederin, in ivy leaf extracts using high-performance liquid chromatography with diode array detector was developed. The chromatographic separation was performed on a YMC Hydrosphere C18 analytical column using a gradient elution of 0.1% phosphoric acid and acetonitrile. The method was validated in terms of specificity, linearity (r(2) > 0.9999), precision [relative standard deviation (RSD) < 0.36%] and accuracy (97.4-103.8%). The limit of detection and limit of quantification were <20.32 and 61.56 ng for all analytes, respectively. The tested compounds were found to be stable in the ivy leaf extract from 0 to 48 h, and the RSD value for each compound was <0.90%. The validated method was successfully applied to quantify all six compounds in a 30% ethanol ivy leaf extract and 13 ivy leaf extract products. The results showed that all the tested products satisfied the minimum requirement for the content of hederacoside C. However, there were some differences between the contents of other constituents.
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors: Miao
authors: M
authors: Yu
authors: Central R&D Center, Ahn gook Pharm., Seoul 152-848, Korea.
authors: Young June
authors: YJ
authors: Shin
authors: College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon 406-840, Korea.
authors: Nanyoung
authors: N
authors: Kim
authors: College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon 406-840, Korea.
authors: Guijae
authors: G
authors: Yoo
authors: College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon 406-840, Korea.
authors: SeonJu
authors: S
authors: Park
authors: College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon 406-840, Korea kimsh11@yonsei.ac.kr.
authors: Seung Hyun
authors: SH
authors: Kim
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D035981:Hedera / Q000737:chemistry*; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D012503:Saponins