title: EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells.
abstract: EphA2 is persistently overexpressed and functionally changed in numerous human cancers. However, to the best of our knowledge, the role that EphA2 plays in prostate cancer is not entirely clear. To investigate the roles of EphA2 in the development and progression of prostate cancer, the present study initially evaluated the roles of the EphA2 protein in LNCaP prostate cancer cells using recombinant plasmid, western blot analysis, flow cytometry, Matrigel invasion chamber and the cell counting kit-8 assay. An immunohistochemistry assay was also conducted to observe the effects of EphA2 in prostate cancer tissues. The results demonstrated that the LNCaP human prostate cancer cells that were transfected with pcDNA3.1(+) plasmid-mediated pcDNA3.1(+)-EphA2, markedly enhanced the cell growth and invasionin vitro. Additionally, EphA2 was overexpressed in prostate cancer specimens and the expression of EphA2 was significantly associated with Gleason grade, total prostate-specific antigen, advanced clinical stage and lymph node metastasis. Collectively, these results demonstrate that EphA2 is involved in malignant cell behavior and is a potential therapeutic target in human prostate cancer.
authors: Department of Urology, Zhongshan Hospital, Xiamen University, Xiamen, Fujian 361004, P.R. China.
authors: Peijie
authors: P
authors: Chen
authors: Department of Urology, Zhongshan Hospital, Xiamen University, Xiamen, Fujian 361004, P.R. China.
authors: Yan
authors: Y
authors: Huang
authors: Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, P.R. China.
authors: Bo
authors: B
authors: Zhang
authors: Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, P.R. China.
authors: Qiuquan
authors: Q
authors: Wang
authors: Department of Urology, Zhongshan Hospital, Xiamen University, Xiamen, Fujian 361004, P.R. China.
authors: Peiming
authors: P
authors: Bai
keywords: EphA2; LNCaP cell; cell proliferation; prostate cancer; tumor invasion