title: The Neurospora crassa CPS-1 polysaccharide synthase functions in cell wall biosynthesis.
abstract: The Neurospora crassa cps-1 gene encodes a polysaccharide synthase with homology to the Cryptococcus neoformans hyaluronic acid synthase Cps1p. Homologs of the cps-1 gene are found in the genomes of many fungi. Loss of CPS-1 results in a cell wall defect that affects all stages of the N. crassa life cycle, including vegetative growth, protoperithecia (female mating structure) development, and conidia (asexual spore) development. The cell wall of cps-1 deletion mutants is sensitive to cell wall perturbation reagents. Our results demonstrate that CPS-1 is required for the incorporation of cell wall proteins into the cell wall and plays a critical role in cell wall biogenesis. We found that the N. crassa cell wall is devoid of hyaluronic acid, and conclude that the polysaccharide produced by the CPS-1 is not hyaluronic acid.
authors: Dept. of Biological Sciences, SUNY, University at Buffalo, Buffalo, NY 14260, United States.
authors: Ci
authors: C
authors: Fu
authors: Dept. of Biological Sciences, SUNY, University at Buffalo, Buffalo, NY 14260, United States.
authors: Eleanor
authors: E
authors: Sokolow
authors: Dept. of Biological Sciences, SUNY, University at Buffalo, Buffalo, NY 14260, United States.
authors: Christopher B
authors: CB
authors: Rupert
authors: Dept. of Biological Sciences, SUNY, University at Buffalo, Buffalo, NY 14260, United States. Electronic address: free@buffalo.edu.
authors: Stephen J
authors: SJ
authors: Free
mesh_terms: D002473:Cell Wall / Q000378:metabolism*; D003455:Cryptococcus neoformans / Q000201:enzymology / Q000235:genetics; D017353:Gene Deletion; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D011134:Polysaccharides; D016695:Glycosyltransferases
keywords: Cell wall biogenesis; Cell wall glucan; Cell wall polysaccharide; Fungal cell wall; Neurospora crassa; Polysaccharide synthase