title: Overexpression of p300 correlates with poor prognosis in patients with cutaneous squamous cell carcinoma.
abstract: BACKGROUND
  It has been suggested that the p300 transcriptional coactivator participates in the regulation of a wide range of cell biological processes, and mutations in p300 have been identified in various cancers.
  
  
  OBJECTIVE
  To investigate p300 expression in cutaneous squamous cell carcinoma (cSCC) tissues and its effect on the outcome of patients with cSCC.
  
  
  METHODS
  Immunohistochemistry (IHC) was performed on a tissue microarray to investigate p300 expression levels in cSCC tissues. Receiver operating characteristic (ROC) curve analysis, Kaplan-Meier plots and a Cox proportional hazards regression model were used to analyse the data.
  
  
  RESULTS
  Based on the ROC curves, we defined the cut-off score for high p300 expression as > 55% of tumour cells positively stained. High expression of p300 was observed in 86 of 165 (52·1%) of the cSCC samples and six of 30 (20%) of the adjacent normal skin tissue samples (P < 0·001). High expression of p300 was positively correlated with lymph node metastasis (P = 0·006) and advanced clinical stage (P < 0·001). In univariate survival analysis, high expression of p300 was correlated with poor patient outcomes in terms of recurrence-free survival (P = 0·006) and overall survival (P < 0·001). Moreover, p300 expression was evaluated as an independent prognostic factor in a multivariate analysis (P = 0·004).
  
  
  CONCLUSIONS
  Our results indicate that high p300 expression is associated with aggressive features of cSCC and suggest that p300 expression, as examined by IHC, will be a promising biomarker for predicting clinical outcomes in patients with cSCC.
authors: Department of Dermatology, The First Affiliated Hospital, Sun Yat-Sen University, No. 58, Zhongshan Er Road, Guangzhou, Guangdong, 510080, China.
authors: M-K
authors: MK
authors: Chen
authors: M-Y
authors: MY
authors: Cai
authors: R-Z
authors: RZ
authors: Luo
authors: X
authors: X
authors: Tian
authors: Q-M
authors: QM
authors: Liao
authors: X-Y
authors: XY
authors: Zhang
authors: J-D
authors: JD
authors: Han
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D050881:E1A-Associated p300 Protein / Q000378:metabolism*; D004812:Epidemiologic Methods; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000401:mortality*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012878:Skin Neoplasms / Q000378:metabolism / Q000401:mortality* / Q000473:pathology; D046888:Tissue Array Analysis; D047368:Tumor Burden
chemical_list: D014408:Biomarkers, Tumor; D050881:E1A-Associated p300 Protein; C494360:EP300 protein, human