title: The protective role of myeloid-derived suppressor cells in concanavalin A-induced hepatic injury.
abstract: The mechanism underlying T cell-mediated fulminant hepatitis is not fully understood. In this study, we investigated whether myeloid derived suppressor cells (MDSCs) could prevent the concanavalin A (ConA)-induced hepatitis through suppressing T cell proliferation. We observed an increase in the frequencies of MDSCs in mouse spleen and liver at early stage of ConA treatment, implicating that the MDSCs might be involved in the initial resistance of mice against ConA-mediated inflammation. Subpopulation analysis showed that the MDSCs in liver of ConA-induced mice were mainly granulocytic MDSCs. Adoptive transfer of the bone marrow-derived MDSCs into ConA-treated mice showed that the MDSCs migrated into the liver and spleen where they suppressed T cell proliferation through ROS pathway. In addition, the frequencies of MDSCs in mice were also significantly increased by the treatment with immune suppressor glucocorticoids. Transfer of MDSCs into the regulatory T cell (Treg)-depleted mice showed that the protective effect of MDSCs on ConA-induced hepatitis is Treg-independent. In conclusion, our results demonstrate that MDSCs possess a direct protective role in T cell-mediated hepatitis, and increasing the frequency of MDSCs by either adoptive transfer or glucocorticoid treatment represents a potential cell-based therapeutic strategy for the acute inflammatory disease.
authors: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210093, China.
authors: Wenli
authors: W
authors: Diao
authors: Fangfang
authors: F
authors: Jin
authors: Bing
authors: B
authors: Wang
authors: Chen-Yu
authors: CY
authors: Zhang
authors: Jiangning
authors: J
authors: Chen
authors: Ke
authors: K
authors: Zen
authors: Limin
authors: L
authors: Li
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000276:immunology*; D039481:CD11b Antigen / Q000276:immunology / Q000378:metabolism; D002465:Cell Movement / Q000276:immunology; D049109:Cell Proliferation; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000276:immunology* / Q000517:prevention & control; D003208:Concanavalin A / Q000633:toxicity*; D003907:Dexamethasone / Q000494:pharmacology; D005434:Flow Cytometry; D005938:Glucocorticoids / Q000494:pharmacology; D008099:Liver / Q000276:immunology / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000008:administration & dosage / Q000633:toxicity; D022423:Myeloid Cells / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D019707:Receptors, Chemokine / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D039481:CD11b Antigen; D005938:Glucocorticoids; C503133:Gr-1 protein, mouse; D008934:Mitogens; D019707:Receptors, Chemokine; D003208:Concanavalin A; D003907:Dexamethasone