title: Association of MYO9B gene polymorphisms with inflammatory bowel disease in Chinese Han population.
abstract: OBJECTIVE
  To explore the association of MYO9B gene polymorphisms with clinical phenotypes and intestinal permeability of individuals with inflammatory bowel disease (IBD) in China.
  
  
  METHODS
  A total of 442 IBD patients and 402 healthy volunteers were genotyped for two single nucleotides (rs962917 and rs1545620) using the ligase detection reaction and polymerase chain reaction. Allelic and genotype frequency analyses were performed for the two groups. Intestinal permeability was evaluated using lactulose (L) and mannitol (M) excretion. The association of MYO9B gene polymorphisms with intestinal permeability between the normal and high intestinal permeability groups was analyzed.
  
  
  RESULTS
  Overall, there was no significant difference in the genotypic and allelic frequencies of MYO9B between IBD patients and controls. Although no association was found with ulcerative colitis in the comparison between the subgroups, the frequencies of rs962917 and rs1545620 were different in the Crohn's disease (CD) subgroup with ileocolitis (CC vs CT and TT, P = 0.014; and AA vs AC and CC, P = 0.022, respectively). rs1545620 variants appear to be the genetic susceptibility factor for perianal disease in CD patients (AA vs AC CC, P = 0.029). In addition, the L/M ratio was significantly higher in IBD patients than in controls (0.065 ± 0.013 vs 0.020 ± 0.002, P = 0.02), but no association was found between the MYO9B gene and the L/M ratio in IBD patients.
  
  
  CONCLUSIONS
  MYO9B gene polymorphisms may influence the sub-phenotypic expression of CD in China. No association between these MYO9B polymorphisms and intestinal permeability in IBD patients was found.
authors: Jing Hu, Qiao Mei, Jian Huang, Nai-Zhong Hu, Xiao-Chang Liu, Jian-Ming Xu, Department of Gastroenterology, the First Affiliated Hospital of Anhui Medical University, Key Laboratory of Gastroenterology of Anhui Province, Hefei 230022, Anhui Province, China.
authors: Jing
authors: J
authors: Hu
authors: Jing Hu, Qiao Mei, Jian Huang, Nai-Zhong Hu, Xiao-Chang Liu, Jian-Ming Xu, Department of Gastroenterology, the First Affiliated Hospital of Anhui Medical University, Key Laboratory of Gastroenterology of Anhui Province, Hefei 230022, Anhui Province, China.
authors: Qiao
authors: Q
authors: Mei
authors: Jing Hu, Qiao Mei, Jian Huang, Nai-Zhong Hu, Xiao-Chang Liu, Jian-Ming Xu, Department of Gastroenterology, the First Affiliated Hospital of Anhui Medical University, Key Laboratory of Gastroenterology of Anhui Province, Hefei 230022, Anhui Province, China.
authors: Jian
authors: J
authors: Huang
authors: Jing Hu, Qiao Mei, Jian Huang, Nai-Zhong Hu, Xiao-Chang Liu, Jian-Ming Xu, Department of Gastroenterology, the First Affiliated Hospital of Anhui Medical University, Key Laboratory of Gastroenterology of Anhui Province, Hefei 230022, Anhui Province, China.
authors: Nai-Zhong
authors: NZ
authors: Hu
authors: Jing Hu, Qiao Mei, Jian Huang, Nai-Zhong Hu, Xiao-Chang Liu, Jian-Ming Xu, Department of Gastroenterology, the First Affiliated Hospital of Anhui Medical University, Key Laboratory of Gastroenterology of Anhui Province, Hefei 230022, Anhui Province, China.
authors: Xiao-Chang
authors: XC
authors: Liu
authors: Jing Hu, Qiao Mei, Jian Huang, Nai-Zhong Hu, Xiao-Chang Liu, Jian-Ming Xu, Department of Gastroenterology, the First Affiliated Hospital of Anhui Medical University, Key Laboratory of Gastroenterology of Anhui Province, Hefei 230022, Anhui Province, China.
authors: Jian-Ming
authors: JM
authors: Xu
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D003093:Colitis, Ulcerative / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism; D003424:Crohn Disease / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009218:Myosins / Q000235:genetics* / Q000378:metabolism; D010539:Permeability; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: C113469:myosin IXB; D009218:Myosins
keywords: Crohn’s disease; Genetic susceptibility; Inflammatory bowel disease; Intestinal permeability; MYO9B; Ulcerative colitis