title: Fluid and highly curved model membranes on vertical nanowire arrays.
abstract: Sensing and manipulating living cells using vertical nanowire devices requires a complete understanding of cell behavior on these substrates. Changes in cell function and phenotype are often triggered by events taking place at the plasma membrane, the properties of which are influenced by local curvature. The nanowire topography can therefore be expected to greatly affect the cell membrane, emphasizing the importance of studying membranes on vertical nanowire arrays. Here, we used supported phospholipid bilayers as a model for biomembranes. We demonstrate the formation of fluid supported bilayers on vertical nanowire forests using self-assembly from vesicles in solution. The bilayers were found to follow the contours of the nanowires to form continuous and locally highly curved model membranes. Distinct from standard flat supported lipid bilayers, the high aspect ratio of the nanowires results in a large bilayer surface available for the immobilization and study of biomolecules. We used these bilayers to bind a membrane-anchored protein as well as tethered vesicles on the nanowire substrate. The nanowire-bilayer platform shown here can be expanded from fundamental studies of lipid membranes on controlled curvature substrates to the development of innovative membrane-based nanosensors.
authors: Division of Physical Chemistry, Department of Chemistry, Lund University , P.O. Box 124, SE-22100 Lund, Sweden.
authors: Aleksandra P
authors: AP
authors: Dabkowska
authors: Cassandra S
authors: CS
authors: Niman
authors: Gaëlle
authors: G
authors: Piret
authors: Henrik
authors: H
authors: Persson
authors: Hanna P
authors: HP
authors: Wacklin
authors: Heiner
authors: H
authors: Linke
authors: Christelle N
authors: CN
authors: Prinz
authors: Tommy
authors: T
authors: Nylander