title: Utilization of graphene electrode in transparent microwell arrays for high throughput cell trapping and lysis.
abstract: Here we present a high-throughput, transparent microfluidic device with embedded microwell arrays sandwiched between transparent electrodes made from graphene (at the bottom) and indium tin oxide (at the top) for dielectrophoretic cell trapping and electrical lysis. Graphene suppresses unwanted faradaic reaction effects on the cells and the medium that is typically observed in ITO based electrodes from application of DC field for electrical lysis. This is because graphene is more electrochemically inert than indium tin oxide (ITO) where ITO undergoes reduction-oxidation (redox) reaction in the presence of electrolyte in most standard cell media. This redox process also compromises ITO's electrical properties and optical transparency over multiple use. The presented microfluidic device shows high efficiency for cell trapping and lysis and an electrochemically stable behavior for long operational life.
authors: Department of Electrical and Computer Engineering, 161 College Avenue, Medford, MA 02155, USA.
authors: S Kabiri
authors: SK
authors: Ameri
authors: Department of Electrical and Computer Engineering, 161 College Avenue, Medford, MA 02155, USA.
authors: P K
authors: PK
authors: Singh
authors: Department of Electrical and Computer Engineering, 161 College Avenue, Medford, MA 02155, USA.
authors: S
authors: S
authors: Sonkusale
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D004912:Erythrocytes / Q000166:cytology* / Q000473:pathology; D006108:Graphite / Q000737:chemistry*; D006461:Hemolysis; D006801:Humans; D056656:Lab-On-A-Chip Devices; D010084:Oxidation-Reduction; D017971:Tin Compounds / Q000737:chemistry; D046888:Tissue Array Analysis / Q000295:instrumentation*
chemical_list: D017971:Tin Compounds; C109984:indium tin oxide; D006108:Graphite
keywords: Cell trapping; Dielectrophoresis; Graphene; Lab-on-chip; Lysis; Redox reaction