title: Hepatic transcriptional analysis in rats treated with Cassia occidentalis seed: involvement of oxidative stress and impairment in xenobiotic metabolism as a putative mechanism of toxicity.
abstract: The present study was undertaken to investigate the effect of Cassia occidentalis (CO) seeds on the transcriptional expression patterns of mRNAs in rat liver by microarray analysis. The results indicated that exposure of CO (0.5%) seeds in diet to rats differentially regulated 60 transcripts belonging to various metabolic pathways including, oxidative stress, xenobiotic metabolism, carbohydrate metabolism, cell cycle, apoptosis etc. The expression of AKT1, CAT, SOD1, CYP1A1, CYP2B1, TGF-β, BAX, CREB1, JNK1 and IL-6 were validated by the qRT-PCR. In addition, involvement of oxidative stress was observed due to marked depletion of glutathione, increase in lipid peroxidation and modulation of antioxidant enzymes in hepatic tissue of rats treated with 0.5-2.0% CO in diet. Furthermore, significant decrease in the levels of Phase 1 (EROD, MROD and PROD) and Phase 2 (QR and GST) enzymes following 0.5-2.0% CO exposure indicates the impairment of xenobiotic metabolism and possible accumulation of toxic ingredients of the seeds in liver. Overall, the study predicts the involvement of multiple pathways and related biomolecules in CO induced hepatotoxicity and the data may be useful in formulating strategies for therapeutic interventions of suspected CO poisoning study cases.
authors: Food, Drug and Chemical Toxicology Division, Council of Scientific and Industrial Research-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow,Uttar Pradesh, India; Department of Medical Elementology and Toxicology, Jamia Hamdard, New Delhi, India.
authors: Gati Krushna
authors: GK
authors: Panigrahi
authors: Food, Drug and Chemical Toxicology Division, Council of Scientific and Industrial Research-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow,Uttar Pradesh, India.
authors: Ashish
authors: A
authors: Yadav
authors: Developmental Toxicology Division, CSIR-IITR, Lucknow, Uttar Pradesh, India.
authors: Anuradha
authors: A
authors: Yadav
authors: Food, Drug and Chemical Toxicology Division, Council of Scientific and Industrial Research-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow,Uttar Pradesh, India.
authors: Kausar M
authors: KM
authors: Ansari
authors: Developmental Toxicology Division, CSIR-IITR, Lucknow, Uttar Pradesh, India.
authors: Rajnish K
authors: RK
authors: Chaturvedi
authors: Mangla Hospital and Research Centre, Bijnor, Uttar Pradesh, India.
authors: Vipin M
authors: VM
authors: Vashistha
authors: Department of Medical Elementology and Toxicology, Jamia Hamdard, New Delhi, India.
authors: S
authors: S
authors: Raisuddin
authors: Food, Drug and Chemical Toxicology Division, Council of Scientific and Industrial Research-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow,Uttar Pradesh, India. Electronic address: mditrc@rediffmail.com.
authors: Mukul
authors: M
authors: Das
mesh_terms: D000818:Animals; D002366:Cassia / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D007202:Indicators and Reagents; D007527:Isoenzymes / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D046228:Microarray Analysis; D006899:Mixed Function Oxygenases / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D012639:Seeds; D015398:Signal Transduction / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects*; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D007202:Indicators and Reagents; D007527:Isoenzymes; D015262:Xenobiotics; D008315:Malondialdehyde; D012313:RNA; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases
keywords: Cassia occidentalis seed; Children; Microarray; Oxidative stress; Xenobiotic metabolism