title: Factors influencing in vitro shoot regeneration from leaf segments of Chrysanthemum.
abstract: The objective of this research was to develop an efficient protocol for shoot regeneration from leaf segments of the Chrysanthemum cv. Vivid Scarlet by examining the effects of plant growth regulators, dark incubation period, gelling agents, and silver nitrate. The highest number of shoots per explant (12.3) was regenerated from leaf explants cultured on Murashige and Skoog (MS) medium supplemented with a combination of 1 mgL(-1) of 6-benzyladenine (BA) and 2 mgL(-1) of α-naphthaleneacetic acid (NAA) under light conditions without any initial dark period. Gelrite was the most effective gelling agent for shoot regeneration among those tested, whereas the presence of silver nitrate distinctly inhibited shoot regeneration. Superior plant growth and rooting was observed on a hormone-free MS medium solidified with Gelrite. Flow cytometry analysis revealed no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions. The established protocol was applicable to shoot regeneration for four out of six cultivars tested. This research will facilitate the genetic transformation and micropropagation of Chrysanthemum cultivars.
authors: Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: naingaunghtay2008@gmail.com.
authors: Aung Htay
authors: AH
authors: Naing
authors: Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Su Min
authors: SM
authors: Jeon
authors: Department of Ecological Environment, Kyungpook National University, Sangju 742-711, Republic of Korea.
authors: Jeung-Sul
authors: JS
authors: Han
authors: National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors: Sun Hyung
authors: SH
authors: Lim
authors: Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Ki Byung
authors: KB
authors: Lim
authors: Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: ckkim@knu.ac.kr.
authors: Chang Kil
authors: CK
authors: Kim
mesh_terms: D031202:Chrysanthemum / Q000254:growth & development*; D003624:Darkness; D005782:Gels; D005838:Genotype; D066298:In Vitro Techniques; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development*; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development*; D011003:Ploidies; D012038:Regeneration / Q000187:drug effects; D012835:Silver Nitrate / Q000494:pharmacology
chemical_list: D005782:Gels; D010937:Plant Growth Regulators; D012835:Silver Nitrate
keywords: Flow cytometry; Gelling agent; Ornamental plant; Plant growth regulator; Silver nitrate