title: Wogonin attenuates ovalbumin antigen-induced neutrophilic airway inflammation by inhibiting th17 differentiation.
abstract: Allergic airway inflammation is generally considered to be a Th2-type immune response. Recent studies, however, have demonstrated that Th17-type immune responses also play important roles in this process, particularly in the pathogenesis of neutrophilic airway inflammation, a hallmark of severe asthma. We scrutinized several Kampo extracts that reportedly exhibit anti-inflammatory activity by using in vitro differentiation system of human and mouse naïve T cells. We found that hange-shashin-to (HST) and oren-gedoku-to (OGT) possess inhibitory activity for Th17 responses in vitro. Indeed, wogonin and berberine, major components common to HST and OGT, exhibit Th17-inhibitory activities in both murine and human systems in vitro. We therefore evaluated whether wogonin suppresses OVA-induced neutrophilic airway inflammation in OVA TCR-transgenic DO11.10 mice. Consequently, oral administration of wogonin significantly improved OVA-induced neutrophilic airway inflammation. Wogonin suppressed the differentiation of naïve T cells to Th17 cells, while showing no effects on activated Th17 cells.
authors: Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, Japan.
authors: Rie
authors: R
authors: Takagi
authors: Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, Japan.
authors: Masaaki
authors: M
authors: Kawano
authors: Department of Respiratory Medicine, Saitama Medical University, Saitama 350-0495, Japan ; Allergy Center, Saitama Medical University, Saitama 350-0495, Japan.
authors: Kazuyuki
authors: K
authors: Nakagome
authors: Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, Japan.
authors: Kumiko
authors: K
authors: Hashimoto
authors: Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, Japan.
authors: Takehiro
authors: T
authors: Higashi
authors: Tsumura Research Laboratories, Tsumura & Co., Ibaraki 300-1192, Japan.
authors: Katsuya
authors: 0000-0001-6756-3260
authors: K
authors: Ohbuchi
authors: Tsumura Research Laboratories, Tsumura & Co., Ibaraki 300-1192, Japan.
authors: Atsushi
authors: 0000-0003-4392-912X
authors: A
authors: Kaneko
authors: Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, Japan ; Allergy Center, Saitama Medical University, Saitama 350-0495, Japan.
authors: Sho
authors: 0000-0003-4810-1065
authors: S
authors: Matsushita