title: Isolation and functional characterization of a novel rice constitutive promoter.
abstract: A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineering. In this report, we isolated a novel promoter, Oscon1 promoter (P OsCon1 ), from the 5' upstream region of a constitutively expressed rice gene OsDHAR1. In P OsCon1 ::GUS transgenic rice, we showed that P OsCon1 had a broad expression spectrum in all tested tissues. The expression of the promoter was further analyzed in comparison with the previously characterized strong constitutive promoters. P OsCon1 exhibited comparable activity to OsCc1, OsAct1 or ZmUbi promoters in most tissues, and more active than 35S promoter in roots, seeds, and calli. Further quantitative assays indicated that P OsCon1 activity was not affected by developmental stages or by environmental factors. Further, 5'-deletions analysis indicated that the distinct regions might contribute to the strong expression of P OsCon1 in different tissues. Overall, our results suggest that P OsCon1 is a novel constitutive promoter, which could potentially use in transgenic crop development.
authors: Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei, 230031, China.
authors: Juan
authors: J
authors: Li
authors: Rong-Fang
authors: RF
authors: Xu
authors: Rui-Ying
authors: RY
authors: Qin
authors: Hui
authors: H
authors: Ma
authors: Hao
authors: H
authors: Li
authors: Ying-Ping
authors: YP
authors: Zhang
authors: Li
authors: L
authors: Li
authors: Peng-Cheng
authors: PC
authors: Wei
authors: Jian-Bo
authors: JB
authors: Yang
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D010940:Plant Proteins