title: Identification of occult Fusobacterium nucleatum central nervous system infection by use of PCR-electrospray ionization mass spectrometry.
abstract: Anaerobic bacteria are often difficult to detect, especially after the initiation of antibiotics. We describe the application of PCR-electrospray ionization mass spectrometry (PCR/ESI-MS) using a sample of cerebrospinal fluid to identify an anaerobic Gram-negative bacillus, Fusobacterium nucleatum, in a patient with "culture-negative" meningitis and cerebral abscesses.
authors: Department of Medicine, Division of Infectious Disease and HIV Medicine, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio, USA.
authors: Sudha
authors: S
authors: Nagalingam
authors: Department of Medicine, Division of Infectious Disease and HIV Medicine, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio, USA.
authors: Michelle
authors: M
authors: Lisgaris
authors: Department of Medicine, Division of Infectious Disease and HIV Medicine, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio, USA.
authors: Benigno
authors: B
authors: Rodriguez
authors: Department of Pathology, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio, USA.
authors: Michael R
authors: MR
authors: Jacobs
authors: Department of Medicine, Division of Infectious Disease and HIV Medicine, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio, USA.
authors: Michael
authors: M
authors: Lederman
authors: Department of Medicine, Division of Infectious Disease and HIV Medicine, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio, USA.
authors: Robert A
authors: RA
authors: Salata
authors: Department of Medicine, Division of Infectious Disease and HIV Medicine, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio, USA Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
authors: Andrea M
authors: AM
authors: Hujer
authors: Infectious Diseases Pathology Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors: Atis
authors: A
authors: Muehlenbachs
authors: Infectious Diseases Pathology Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors: Marlene
authors: M
authors: DeLeon-Carnes
authors: Department of Medicine, University of Illinois School of Medicine, Peoria, Illinois, USA.
authors: John J
authors: JJ
authors: Farrell
authors: Ibis Biosciences, an Abbott Company, Carlsbad, California, USA.
authors: Rangarajan
authors: http://orcid.org/0000-0002-2951-6372
authors: R
authors: Sampath
authors: Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA Departments of Pharmacology, Microbiology and Molecular Biology, Case Western Reserve University, Cleveland, Ohio, USA Robert.bonomo@va.gov.
authors: Robert A
authors: RA
authors: Bonomo
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001706:Biopsy; D001922:Brain Abscess / Q000150:complications / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D005674:Fusobacterium Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D016967:Fusobacterium nucleatum / Q000302:isolation & purification*; D006257:Head / Q000000981:diagnostic imaging; D006651:Histocytochemistry; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D016920:Meningitis, Bacterial / Q000150:complications / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D008853:Microscopy; D016133:Polymerase Chain Reaction; D011859:Radiography; D021241:Spectrometry, Mass, Electrospray Ionization; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents