title: Mass spectrometry for proteomics-based investigation using the zebrafish vertebrate model system.
abstract: The zebrafish (Danio rerio) is frequently being used to investigate the genetics of human diseases as well as resulting pathologies. Ease of both forward and reverse genetic manipulation along with conservation of vertebrate organ systems and disease causing genes has made this system a popular model. Many techniques have been developed to manipulate the genome of zebrafish producing mutants in a vast array of genes. While genetic manipulation of zebrafish has progressed, proteomics have been under-utilized. This review highlights studies that have already been performed using proteomic techniques and as well as our initial proteomic work comparing changes to the proteome between the ascl1a-/- and WT intestine.
authors: Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY, 13699-5810, USA.
authors: Reshica
authors: R
authors: Baral
authors: Armand G
authors: AG
authors: Ngounou Wetie
authors: Costel C
authors: CC
authors: Darie
authors: Kenneth N
authors: KN
authors: Wallace
mesh_terms: D000818:Animals; D016678:Genome / Q000502:physiology*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D009154:Mutation; D040901:Proteomics / Q000379:methods*; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029961:Zebrafish Proteins