title: In vitro regeneration of Coelogyne nervosa A.Rich. and Eria pseudoclavicaulis Blatt., threatened orchids of Western Ghats, India.
abstract: The seeds of C. nervosa and E. pseudoclavicaulis were germinated asymbiotically on Knudson C (KC) and Schenk and Hildebrandt basal medium (SH). Growth regulators such as 2,4-Dichlorophenoxyacetic acid (2,4-D) individually and in combinations with benzyladenine (BA) and kinetin were used for callus induction from the protocorm like bodies. Coelogyne nervosa showed maximum (90%) callus induction in Knudson C medium supplemented with 2,4-D (2.26 microM) and Eria pseudoclavicaulis showed 60% callus induction in Schenk and Hildebrandt medium supplemented with 2,4-D (2.26 microM). Calli developed a route of production of protocorm-like bodies and eventually developed into plantlets on transfer to growth regulator free half strength basal medium. The well rooted plants were hardened successfully in the potting mixture containing coconut husk, charcoal, and brick pieces in the ratio 2:1:1.
authors: B Sahaya
authors: BS
authors: Shibu
authors: P Servin
authors: PS
authors: Wesley
authors: Moin
authors: M
authors: Sarmad
authors: B Chitra
authors: BC
authors: Devi
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D056727:Endangered Species; D007194:India; D029595:Orchidaceae / Q000166:cytology / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D012038:Regeneration / Q000187:drug effects
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators; D015084:2,4-Dichlorophenoxyacetic Acid