title: 1HNMR-based metabolomic profile of rats with experimental acute pancreatitis.
abstract: BACKGROUND
  Acute pancreatitis (AP) is a common inflammatory disease of the pancreas accompanied by serious metabolic disturbances. Nevertheless, the specific metabolic process of this disease is still unclear. Characterization of the metabolome may help identify biomarkers for AP. To identify potential biomarkers, this study therefore investigated the 1H-nuclear magnetic resonance (NMR)-based metabolomic profile of AP.
  
  
  METHODS
  Fourteen male adult Sprague-Dawley rats were randomized into two groups: the AP group, in which AP was induced by retrograde ductal infusion of 3.5% sodium taurocholate; and the sham operation group (SO), in which rats were infused with 0.9% saline. Blood samples were obtained 12 hours later and a 600 MHz superconducting NMR spectrometer was used to detect plasma metabolites. Principal components analysis (PCA) and partial least squares-discriminant analysis after orthogonal signal correction (OSC-PLS-DA) were used to analyze both longitudinal Eddy-delay (LED) and Carr-Purcell-Meiboom-Gill (CPMG) spectra.
  
  
  RESULTS
  Differences in plasma metabolites between the two groups were detected by PCA and PLS-DA of 1HNMR spectra. Compared with the SO group, plasma levels of lactate (δ 1.3, 1.34, 4.1), valine (δ 0.98, 1.02), succinic acid (δ 2.38), 3-hydroxybutyric acid (3-HB, δ 1.18), high density lipoprotein (HDL, δ 0.8), and unsaturated fatty acid (UFA, δ 2.78, 5.3) were elevated in the AP group, while levels of glycerol (δ 3.58, 3.66), choline (δ 3.22), trimethylamine oxide (TMAO, δ 3.26), glucose (δ 3-4), glycine (δ 3.54), very low density lipoprotein (VLDL, δ 1.34) and phosphatidylcholine (Ptd, δ 2.78) were decreased.
  
  
  CONCLUSIONS
  AP has a characteristic metabolic profile. Lactate, valine, succinic acid, 3-HB, HDL, UFA, glycerol, choline, TMAO, glucose, glycine, VLDL, and Ptd may be potential biomarkers of early stage AP.
authors: Juan
authors: J
authors: Li
authors: Xian-lin
authors: XL
authors: Zhao
authors: Yi-xia
authors: YX
authors: Liu
authors: Xiao-hang
authors: XH
authors: Peng
authors: Shi-feng
authors: SF
authors: Zhu
authors: Hui
authors: H
authors: Guo
authors: Yi-ling
authors: YL
authors: Liu
authors: Mei-hua
authors: MH
authors: Wan
authors: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China. wftang900@gmail.com.
authors: Wen-fu
authors: WF
authors: Tang
mesh_terms: D000818:Animals; D002756:Cholagogues and Choleretics; D016002:Discriminant Analysis; D016018:Least-Squares Analysis; D008297:Male; D055442:Metabolome; D010195:Pancreatitis / Q000097:blood* / Q000139:chemically induced; D025341:Principal Component Analysis; D066244:Proton Magnetic Resonance Spectroscopy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013656:Taurocholic Acid
chemical_list: D002756:Cholagogues and Choleretics; D013656:Taurocholic Acid