title: Combined DNA-RNA fluorescent in situ hybridization (FISH) to study X chromosome inactivation in differentiated female mouse embryonic stem cells.
abstract: Fluorescent in situ hybridization (FISH) is a molecular technique which enables the detection of nucleic acids in cells. DNA FISH is often used in cytogenetics and cancer diagnostics, and can detect aberrations of the genome, which often has important clinical implications. RNA FISH can be used to detect RNA molecules in cells and has provided important insights in regulation of gene expression. Combining DNA and RNA FISH within the same cell is technically challenging, as conditions suitable for DNA FISH might be too harsh for fragile, single stranded RNA molecules. We here present an easily applicable protocol which enables the combined, simultaneous detection of Xist RNA and DNA encoded by the X chromosomes. This combined DNA-RNA FISH protocol can likely be applied to other systems where both RNA and DNA need to be detected.
authors: Department of Reproduction and Development, Erasmus MC - University Medical Center; t.barakat@erasmusmc.nl.
authors: Tahsin Stefan
authors: TS
authors: Barakat
authors: Department of Reproduction and Development, Erasmus MC - University Medical Center.
authors: Joost
authors: J
authors: Gribnau
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D004247:DNA / Q000032:analysis*; D053595:Embryonic Stem Cells / Q000502:physiology*; D005260:Female; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D051379:Mice; D012313:RNA / Q000032:analysis*; D049951:X Chromosome Inactivation
chemical_list: D012313:RNA; D004247:DNA