title: Effects of celecoxib on proliferation and tenocytic differentiation of tendon-derived stem cells.
abstract: NSAIDs are often ingested to reduce the pain and improve regeneration of tendon after tendon injury. Although the effects of NSAIDs in tendon healing have been reported, the data and conclusions are not consistent. Recently, tendon-derived stem cells (TDSCs) have been isolated from tendon tissues and has been suggested involved in tendon repair. Our study aims to determine the effects of COX-2 inhibitor (celecoxib) on the proliferation and tenocytic differentiation of TDSCs. TDSCs were isolated from mice Achilles tendon and exposed to celecoxib. Cell proliferation rate was investigated at various concentrations (0.1, 1, 10 and 100 μg/ml) of celecoxib by using hemocytometer. The mRNA expression of tendon associated transcription factors, tendon associated collagens and tendon associated molecules were determined by reverse transcription-polymerase chain reaction. The protein expression of Collagen I, Collagen III, Scleraxis and Tenomodulin were determined by Western blotting. The results showed that celecoxib has no effects on TDSCs cell proliferation in various concentrations (p>0.05). The levels of most tendon associated transcription factors, tendon associated collagens and tendon associated molecules genes expression were significantly decreased in celecoxib (10 μg/ml) treated group (p<0.05). Collagen I, Collagen III, Scleraxis and Tenomodulin protein expression were also significantly decreased in celecoxib (10 μg/ml) treated group (p<0.05). In conclusion, celecoxib inhibits tenocytic differentiation of tendon-derived stem cells but has no effects on cell proliferation.
authors: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Kairui
authors: K
authors: Zhang
authors: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Sheng
authors: S
authors: Zhang
authors: Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.
authors: Qianqian
authors: Q
authors: Li
authors: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Department of Orthopaedics, 421 Hospital of PLA, Guangzhou 510318, China.
authors: Jun
authors: J
authors: Yang
authors: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Department of Orthopaedics, The First Affiliated Hospital to Guangzhou Medical University, Guangzhou 510120, China.
authors: Weiqiang
authors: W
authors: Dong
authors: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Shengnan
authors: S
authors: Wang
authors: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Yirong
authors: Y
authors: Cheng
authors: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Mohammed
authors: M
authors: Al-Qwbani
authors: Department of Orthopaedics, Subei People's Hospital of Jiangsu Province (Clinical Medical College of Yangzhou University), Yangzhou, Jiangsu Province 225001, China. Electronic address: 1780468505@qq.com.
authors: Qiang
authors: Q
authors: Wang
authors: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: carryzhang1985@live.com.
authors: Bin
authors: B
authors: Yu
mesh_terms: D000818:Animals; D000068579:Celecoxib; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D052246:Cyclooxygenase 2 Inhibitors / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D051379:Mice; D008810:Mice, Inbred C57BL; D011720:Pyrazoles / Q000008:administration & dosage*; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D013449:Sulfonamides / Q000008:administration & dosage*; D013710:Tendons / Q000166:cytology* / Q000502:physiology*
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D011720:Pyrazoles; D013449:Sulfonamides; D003094:Collagen; D000068579:Celecoxib
keywords: Celecoxib; Cell proliferation; Tendon-derived stem cells; Tenocytic differentiation