title: miR-182 targets CHL1 and controls tumor growth and invasion in papillary thyroid carcinoma.
abstract: In this study, we investigated the role and underlying mechanism of action of miR-182 in papillary thyroid carcinoma (PTC). Bioinformatics analysis revealed close homolog of LI (CHL1) as a potential target of miR-182. Upregulation of miR-182 was significantly correlated with CHL1 downregulation in human PTC tissues and cell lines. miR-182 suppressed the expression of CHL1 mRNA through direct targeting of the 3'-untranslated region (3'-UTR). Downregulation of miR-182 suppressed growth and invasion of PTC cells. Silencing of CHL1 counteracted the effects of miR-182 suppression, while its overexpression mimicked these effects. Our data collectively indicate that miR-182 in PTC promotes cell proliferation and invasion through direct suppression of CHL1, supporting the potential utility of miR-182 inhibition as a novel therapeutic strategy against PTC.
authors: Department of Endocrine, Shanghai Pudong New Area Gongli Hospital, Shanghai, China.
authors: Hongling
authors: H
authors: Zhu
authors: Department of Endocrine, The 118th Hospital of Chinese PLA, Wenzhou, Zhejiang, China.
authors: Jin
authors: J
authors: Fang
authors: Department of Endocrine, Shanghai Pudong New Area Gongli Hospital, Shanghai, China.
authors: Jichen
authors: J
authors: Zhang
authors: Department of Endocrine, Shanghai Pudong New Area Gongli Hospital, Shanghai, China.
authors: Zefei
authors: Z
authors: Zhao
authors: Department of Endocrine, Shanghai Pudong New Area Gongli Hospital, Shanghai, China.
authors: Lianyong
authors: L
authors: Liu
authors: Department of Endocrine, Shanghai Pudong New Area Gongli Hospital, Shanghai, China.
authors: Jingnan
authors: J
authors: Wang
authors: Department of Endocrine, Shanghai Pudong New Area Gongli Hospital, Shanghai, China.
authors: Qian
authors: Q
authors: Xi
authors: Department of Endocrine, Shanghai Pudong New Area Gongli Hospital, Shanghai, China. Electronic address: mjgugonglihos@yeah.net.
authors: Mingjun
authors: M
authors: Gu
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism*; D002291:Carcinoma, Papillary; D049109:Cell Proliferation; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D035683:MicroRNAs; C538984:Mirn182 microRNA, human
keywords: CHL1; Invasion; Papillary thyroid carcinoma; Proliferation; miR-182