title: Effects of interactions between progesterone and prostaglandin on uterine contractility in a perfused swine uterus model.
abstract: OBJECTIVE
  Uterine quiescence at the time of embryo transfer is a prerequisite for successful in vitro fertilization (IVF). This study assessed whether prostaglandin-induced contractions in the perfused swine uterus can be reduced by progesterone.
  
  
  METHODS
  Fifty-eight non-pregnant swine uteri were perfused using an established extracorporeal perfusion model. Intrauterine pressure changes during perfusion with prostaglandin (PG) administration (PGE1, PGE2, PGF2α) and progesterone (1 pg/ml, 10 pg/ml, 25 pg/ml, 50 pg/ml) were assessed using an intrauterine double-chip microcatheter.
  
  
  RESULTS
  The contraction-stimulating effect of PGs was clearly reduced by progesterone. Only PGE1 still triggered relevant contractions during continuous perfusion with progesterone solution, up to a concentration of 10 pg/ml. With PGE2 and PGF2α, a clear reduction of uterine contractility was observed even at at a progesterone concentration of 1 pg/ml.
  
  
  CONCLUSIONS
  The extracorporal perfusion model of swine uteri shows that PG-induced contractions can be reduced in a dose-dependent manner by progesterone.
authors: Department of Gynaecology, Erlangen University Hospital, Erlangen, Germany.
authors: Julian
authors: J
authors: Künzel
authors: Department of Gynaecology, Erlangen University Hospital, Erlangen, Germany.
authors: Klaudija
authors: K
authors: Geisler
authors: Department of Gynaecology, Erlangen University Hospital, Erlangen, Germany.
authors: Theodoros
authors: T
authors: Maltaris
authors: Department of Gynaecology, Erlangen University Hospital, Erlangen, Germany.
authors: Andreas
authors: A
authors: Müller
authors: Department of Gynaecology, Erlangen University Hospital, Erlangen, Germany.
authors: Inge
authors: I
authors: Hoffmann
authors: Department of Gynaecology, Erlangen University Hospital, Erlangen, Germany.
authors: Heike
authors: H
authors: Schneider
authors: Department of Gynaecology, Erlangen University Hospital, Erlangen, Germany.
authors: Matthias W
authors: MW
authors: Beckmann
authors: Department of Gynaecology, Erlangen University Hospital, Erlangen, Germany ralf.dittrich@uk-erlangen.de.
authors: Ralf
authors: R
authors: Dittrich
authors: Department of Gynaecology, Erlangen University Hospital, Erlangen, Germany.
authors: Patricia G
authors: PG
authors: Oppelt
mesh_terms: D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D007552:Isotonic Solutions; D010477:Perfusion; D011374:Progesterone / Q000378:metabolism* / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism* / Q000494:pharmacology; D013552:Swine; D014590:Uterine Contraction / Q000187:drug effects / Q000502:physiology*; D014599:Uterus / Q000187:drug effects / Q000502:physiology*
chemical_list: D007552:Isotonic Solutions; D011453:Prostaglandins; D011374:Progesterone
keywords: Swine uterus; in vitro fertilization; perfusion model; progesterone; prostaglandins