title: Comparative analysis of DNA polymorphisms and phylogenetic relationships among Syzygium cumini Skeels based on phenotypic characters and RAPD technique.
abstract: The Indian black berry (Syzygium cumini Skeels) has a great nutraceutical and medicinal properties. As in other fruit crops, the fruit characteristics are important attributes for differentiation were also determined for different accessions of S. cumini. The fruit weight, length, breadth, length: breadth ratio, pulp weight, pulp content, seed weight and pulp: seed ratio significantly varied in different accessions. Molecular characterization was carried out using PCR based RAPD technique. Out of 80 RAPD primers, only 18 primers produced stable polymorphisms that were used to examine the phylogenetic relationship. A sum of 207 loci were generated out of which 201 loci found polymorphic. The average genetic dissimilarity was 97 per cent among jamun accessions. The phylogenetic relationship was also determined by principal coordinates analysis (PCoA) that explained 46.95 per cent cumulative variance. The two-dimensional PCoA analysis showed grouping of the different accessions that were plotted into four sub-plots, representing clustering of accessions. The UPGMA (r = 0.967) and NJ (r = 0.987) dendrogram constructed based on the dissimilarity matrix revealed a good degree of fit with the cophenetic correlation value. The dendrogram grouped the accessions into three main clusters according to their eco-geographical regions which given useful insight into their phylogenetic relationships.
authors: Division of Crop Improvement and Biotechnology, Central Institute for Subtropical Horticulture, Rehmankhera, P.O. Kakori, Lucknow - 226 101 (U.P.), India.
authors: Jitendra P
authors: JP
authors: Singh
authors: Division of Crop Improvement and Biotechnology, Central Institute for Subtropical Horticulture, Rehmankhera, P.O. Kakori, Lucknow - 226 101 (U.P.), India.
authors: Ak
authors: A
authors: Singh
authors: Division of Crop Improvement and Biotechnology, Central Institute for Subtropical Horticulture, Rehmankhera, P.O. Kakori, Lucknow - 226 101 (U.P.), India.
authors: Anju
authors: A
authors: Bajpai
authors: Department of Bioengineering, Integral University, Kursi Road, Dasauli, Lucknow-226 026, Uttar Pradesh, India.
authors: Iffat Zareen
authors: IZ
authors: Ahmad
keywords: PCoA; RAPD; Syzygium cumini Skeels; UPGMA and NJ dendrogram; jaccard coefficients; phenotypic characters