title: The virion of Cafeteria roenbergensis virus (CroV) contains a complex suite of proteins for transcription and DNA repair.
abstract: Cafeteria roenbergensis virus (CroV) is a giant virus of the Mimiviridae family that infects the marine phagotrophic flagellate C. roenbergensis. CroV possesses a DNA genome of ~730 kilobase pairs that is predicted to encode 544 proteins. We analyzed the protein composition of purified CroV particles by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and identified 141 virion-associated CroV proteins and 60 host proteins. Data are available via ProteomeXchange with identifier PXD000993. Predicted functions could be assigned to 36% of the virion proteins, which include structural proteins as well as enzymes for transcription, DNA repair, redox reactions and protein modification. Homologs of 36 CroV virion proteins have previously been found in the virion of Acanthamoeba polyphaga mimivirus. The overlapping virion proteome of CroV and Mimivirus reveals a set of conserved virion protein functions that were presumably present in the last common ancestor of the Mimiviridae.
authors: Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada V6T 1Z4. Electronic address: mfischer@mpimf-heidelberg.mpg.de.
authors: Matthias G
authors: MG
authors: Fischer
authors: Department of Biochemistry & Molecular Biology and Centre for High-Throughput Biology, University of British Columbia, Vancouver, Canada V6T 1Z4.
authors: Isabelle
authors: I
authors: Kelly
authors: Department of Biochemistry & Molecular Biology and Centre for High-Throughput Biology, University of British Columbia, Vancouver, Canada V6T 1Z4.
authors: Leonard J
authors: LJ
authors: Foster
authors: Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada V6T 1Z4; Department of Botany, University of British Columbia, Vancouver, Canada V6T 1Z4; Department of Earth & Ocean Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4.
authors: Curtis A
authors: CA
authors: Suttle
mesh_terms: D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D004260:DNA Repair; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D056686:Mimiviridae / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs; D010084:Oxidation-Reduction; D011401:Promoter Regions, Genetic / Q000235:genetics; D020543:Proteome; D058009:Stramenopiles / Q000821:virology*; D053719:Tandem Mass Spectrometry; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D056224:Viral Structures; D014771:Virion / Q000235:genetics / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D020543:Proteome; D014764:Viral Proteins
keywords: Cafeteria roenbergensis virus; CroV; Giant virus; LC–MS/MS; Megavirales; Mimivirus; NCLDV; Proteome; Virion; Virophage