title: U-Bang-Haequi Tang: A Herbal Prescription that Prevents Acute Inflammation through Inhibition of NF-κB-Mediated Inducible Nitric Oxide Synthase.
abstract: Since antiquity, medical herbs have been prescribed for both treatment and preventative purposes. Herbal formulas are used to reduce toxicity as well as increase efficacy in traditional Korean medicine. U-bang-haequi tang (UBT) is a herbal prescription containing Arctii fructus and Forsythia suspensa as its main components and has treated many human diseases in traditional Korean medicine. This research investigated the effects of UBT against an acute phase of inflammation. For this, we measured induction of nitric oxide (NO) and related proteins in macrophage cell line stimulated by lipopolysaccharide (LPS). Further, paw swelling was measured in carrageenan-treated rats. Carrageenan significantly induced activation of inflammatory cells and increases in paw volume, whereas oral administration of 0.3 or 1 g/kg/day of UBT inhibited the acute inflammatory response. In RAW264.7 cells, UBT inhibited mRNA and protein expression levels of iNOS. UBT treatment also blocked elevation of NO production, nuclear translocation of NF-κB, phosphorylation of Iκ-Bα induced by LPS. Moreover, UBT treatment significantly blocked the phosphorylation of p38 and c-Jun NH2-terminal kinases by LPS. In conclusion, UBT prevented both acute inflammation in rats as well as LPS-induced NO and iNOS gene expression through inhibition of NF-κB in RAW264.7 cells.
authors: Department of Ophthalmology, Otolaryngology & Dermatology, Daegu Haany University, Daegu 706-828, Republic of Korea.
authors: Min
authors: M
authors: Hwangbo
authors: Medical Research Center for Globalization of Herbal Formulation, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea.
authors: Ji Yun
authors: JY
authors: Jung
authors: College of Pharmacy, Chosun University, Gwangju 501-759, Republic of Korea.
authors: Sung Hwan
authors: SH
authors: Ki
authors: Medical Research Center for Globalization of Herbal Formulation, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea.
authors: Sang Mi
authors: SM
authors: Park
authors: Medical Research Center for Globalization of Herbal Formulation, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea.
authors: Kyung Hwan
authors: KH
authors: Jegal
authors: Medical Research Center for Globalization of Herbal Formulation, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea.
authors: Il Je
authors: 0000-0001-6295-0608
authors: IJ
authors: Cho
authors: Medical Research Center for Globalization of Herbal Formulation, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea.
authors: Ju-Hee
authors: JH
authors: Lee
authors: Sunlin University, Pohang 791-712, Republic of Korea.
authors: Seung Ho
authors: SH
authors: Kang
authors: College of Oriental Medicine, Dongguk University, Gyeongju 780-714, Republic of Korea.
authors: Sun-Dong
authors: SD
authors: Park
authors: Medical Research Center for Globalization of Herbal Formulation, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea.
authors: Sae Kwang
authors: SK
authors: Ku
authors: Medical Research Center for Globalization of Herbal Formulation, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea.
authors: Sang Chan
authors: 0000-0003-0515-0764
authors: SC
authors: Kim
authors: Medical Research Center for Globalization of Herbal Formulation, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea ; Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, China.
authors: Rong Jie
authors: RJ
authors: Zhao
authors: Department of Ophthalmology, Otolaryngology & Dermatology, Daegu Haany University, Daegu 706-828, Republic of Korea.
authors: Seon Young
authors: SY
authors: Jee
authors: Medical Research Center for Globalization of Herbal Formulation, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea ; College of Oriental Medicine, Dongguk University, Gyeongju 780-714, Republic of Korea.
authors: Young Woo
authors: 0000-0002-3323-7106
authors: YW
authors: Kim