title: Use of the DBD-FISH technique for detecting DNA breakage in response to high doses of X-rays.
abstract: The aim of this study was to generate a dose-response curve using the DNA breakage detection-fluorescent in situ hybridization (DBD-FISH) test as a biomarker of initial genetic effects induced by high doses of X-rays. A dose-response curve was obtained by measuring the ex vivo responses to increasing doses (0-50 Gy) of X-rays in the peripheral blood lymphocytes of ten healthy donors. The overall dose-response curve was constructed using integrated density (ID; area × fluorescence intensity) as a measure of genetic damage induced by irradiation. The correlation coefficient was high (r = 0.934, b(0) = 10.408, and b(1) = 0.094). One-way ANOVA with the Student-Newman-Keuls test for multiple comparisons showed significant differences among the average ln ID values according to dose. Our results suggest the usefulness of the DBD-FISH technique for measuring intrinsic individual cellular radio sensitivity ex vivo.
authors: Department of Genetics, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, IMSS, C.P. 64720, Monterrey, NL, Mexico, elvacortes@cibinmty.net.
authors: Elva I
authors: EI
authors: Cortés-Gutiérrez
authors: Martha I
authors: MI
authors: Dávila-Rodríguez
authors: Ricardo M
authors: RM
authors: Cerda-Flores
authors: José Luis
authors: JL
authors: Fernández
authors: Carmen
authors: C
authors: López-Fernández
authors: Jaime
authors: J
authors: Gosálvez
mesh_terms: D000328:Adult; D053960:DNA Breaks / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects; D015203:Reproducibility of Results; D014965:X-Rays / Q000009:adverse effects; D055815:Young Adult