title: The miR-100-mediated pathway regulates apoptosis against virus infection in shrimp.
abstract: The process of programmed cell death process or apoptosis can be regulated by microRNAs, 21-25 nt short non-coding RNAs. However, a comprehensive view of apoptosis-associated miRNAs has not been intensively characterized. In this study, the shrimp miRNA microarray data showed that 199 miRNAs were involved in the regulation of apoptosis, among which 8 miRNAs were evolutionarily conserved in animals. The loss-of-function experiments in vivo in shrimp revealed that miR-100 was served as an anti-apoptosis miRNA through targeting the mRNA of trypsin gene. The results indicated that the silencing of miR-100 expression resulted in the increase of apoptotic activity of shrimp hemocytes and further led to the decreases of virus genome copies in shrimp and virus-infected shrimp mortality compared with the controls. The findings showed that miR-100-trypsin signaling pathway played an important role in the antiviral immunity by regulating apoptosis. Therefore, our study presented a novel miR-100-mediated pathway in the regulation of apoptosis.
authors: Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Lu
authors: L
authors: Yang
authors: Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Geng
authors: G
authors: Yang
authors: Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China. Electronic address: zhang_xiaobo@hotmail.com.
authors: Xiaobo
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D006434:Hemocytes / Q000378:metabolism / Q000821:virology; D007113:Immunity, Innate; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D033561:Penaeidae / Q000235:genetics / Q000276:immunology / Q000502:physiology* / Q000821:virology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Antiviral immunity; Apoptosis; Trypsin; WSSV; miRNA