title: DNA-based detection of mercury(II) ions through characteristic current signals in nanopores with high sensitivity and selectivity.
abstract: We report single-molecule detection of Hg(2+) by threading an Hg(2+)-mediated DNA duplex through α-hemolysin nanopores, which generates characteristic three-level current patterns and enables the unambiguous detection of Hg(2+). This strategy precludes any background interference and features high sensitivity and selectivity. Moreover, the platform could be readily integrated with aptamers and molecular beacons, offering extended possibilities for the construction of a new DNA-based nanopore sensing system.
authors: Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety.
authors: Tao
authors: T
authors: Zeng
authors: Ting
authors: T
authors: Li
authors: Yuru
authors: Y
authors: Li
authors: Lei
authors: L
authors: Liu
authors: Xingyong
authors: X
authors: Wang
authors: Quansheng
authors: Q
authors: Liu
authors: Yuliang
authors: Y
authors: Zhao
authors: Hai-Chen
authors: HC
authors: Wu
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006460:Hemolysin Proteins / Q000737:chemistry*; D007477:Ions; D008628:Mercury / Q000032:analysis*; D008670:Metals / Q000737:chemistry; D058608:Nanopores; D036103:Nanotechnology; D017422:Sequence Analysis, DNA
chemical_list: D006460:Hemolysin Proteins; D007477:Ions; D008670:Metals; D004247:DNA; D008628:Mercury