title: Three-day-old human unfertilized oocytes after in vitro fertilization/intracytoplasmic sperm injection can be activated by calcium ionophore a23187 or strontium chloride and develop to blastocysts.
abstract: Our objective was to observe the effectiveness of the calcium ionophore A23187 or strontium chloride on the activation and subsequent embryonic development of 3-day-old human unfertilized oocytes after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). A total of 279 3-day-old unfertilized oocytes after IVF or ICSI were randomized to be activated by the calcium ionophore A23187 (n=138) or strontium chloride (n=141). The activated oocytes were cultured in vitro for 3-5 days. Activation rate, pronucleus formation, cleavage rate, and developmental potential of parthenotes during culture were evaluated. A total of 170 unfertilized oocytes were activated; 65 developed to cleavage stage, 19 developed to greater than the eight-cell stage, and five blastocysts were obtained. The activation rate of the calcium ionophore A23187 group was higher than that of the strontium chloride group (75.4% and 46.8%, respectively; p<0.05); there was significant difference between two groups (p<0.05). Among the 44 cleaved oocytes in the calcium ionophore A23187 group, eight developed to the two- to four-cell stage, 17 developed to the five- to eight-cell stage, 15 developed to greater than the eight-cell stage, and four blastocysts were obtained. Among the 21 cleaved oocytes in the strontium chloride group, six developed to the two- to four- cell stage, 10 developed to the five- to eight-cell stage, four developed to greater than the eight-cell stage, and one blastocyst was obtained. Three-day-old unfertilized human oocytes after IVF or ICSI could be activated by the calcium ionophore A23187 or strontium chloride, and a small part of parthenogenetic embryos developed into blastocysts. The treatment with the calcium ionophore A23187 was better than that of strontium chloride in respect to the activation rate of 3-day-old unfertilized human oocytes after IVF or ICSI.
authors: Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University , Beijing, China .
authors: Ying
authors: Y
authors: Liu
authors: Xiao-Jie
authors: XJ
authors: Han
authors: Ming-Hui
authors: MH
authors: Liu
authors: Shu-Yu
authors: SY
authors: Wang
authors: Chan-Wei
authors: CW
authors: Jia
authors: Lan
authors: L
authors: Yu
authors: Guoqing
authors: G
authors: Ren
authors: Li
authors: L
authors: Wang
authors: Wei
authors: W
authors: Li
mesh_terms: D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology*; D061207:Calcium Ionophores / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D010312:Parthenogenesis / Q000187:drug effects*; D020554:Sperm Injections, Intracytoplasmic; D013324:Strontium / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D061207:Calcium Ionophores; D000001:Calcimycin; C025700:strontium chloride; D013324:Strontium