title: Anatomical, molecular and pathological consideration of the circumventricular organs.
abstract: OBJECTIVE
  Circumventricular organs (CVOs) are a diverse group of specialised structures characterized by peculiar vascular and position around the third and fourth ventricles of the brain. In humans, these organs are present during the fetal period and some become vestigial after birth. Some, such as the pineal gland (PG), subcommissural organ (SCO) and organum vasculosum of the lamina terminalis (OVLT), which are located around the third ventricle, might be the site of origin of periventricular tumours. In contrast to humans, CVOs are present in the adult rat and can be dissected by laser capture microdissection (LCM).
  
  
  METHODS
  In this study, we used LCM and microarrays to analyse the transcriptomes of three CVOs, the SCO, the subfornical organ (SFO) and the PG and the third ventricle ependyma of the adult rat, in order to better characterise these organs at the molecular level. Furthermore, an immunohistochemical study of Claudin-3 (CLDN3), a membrane protein involved in forming cellular tight junctions, was performed at the level of the SCO.
  
  
  RESULTS
  This study highlighted some potentially new or already described specific markers of these structures as Erbb2 and Col11a1 in ependyma, Epcam and CLDN3 in the SCO, Ren1 and Slc22a3 in the SFO and Tph, Anat and Asmt in the PG. Moreover, we found that CLDN3 expression was restricted to the apical pole of ependymocytes in the SCO.
authors: Service de neurochirurgie pédiatrique, hôpital Pierre-Wertheimer, 59, boulevard Pinel, 69677 Lyon, France; Inserm U1028, CNRS UMR5292, équipe neuro-oncologie et neuro-inflammation, centre de recherche en neurosciences de Lyon, 69372 Lyon, France. Electronic address: alexandru.szathmari@chu-lyon.fr.
authors: A
authors: A
authors: Szathmari
authors: Service de pathologie et de neuropathologie, centre de biologie et pathologie Est, groupement hospitalier Est, hospices civils de Lyon, 69677 Lyon, France; Inserm U1028, CNRS UMR5292, équipe neuro-oncologie et neuro-inflammation, centre de recherche en neurosciences de Lyon, 69372 Lyon, France.
authors: A
authors: A
authors: Jouvet
authors: Service de neurochirurgie pédiatrique, hôpital Pierre-Wertheimer, 59, boulevard Pinel, 69677 Lyon, France.
authors: C
authors: C
authors: Mottolese
authors: Inserm U1028, CNRS UMR5292, équipe neuro-oncologie et neuro-inflammation, centre de recherche en neurosciences de Lyon, 69372 Lyon, France.
authors: J
authors: J
authors: Champier
authors: Inserm U1028, CNRS UMR5292, équipe neuro-oncologie et neuro-inflammation, centre de recherche en neurosciences de Lyon, 69372 Lyon, France.
authors: M
authors: M
authors: Fèvre Montange
mesh_terms: D002552:Cerebral Ventricles / Q000033:anatomy & histology / Q000378:metabolism / Q000473:pathology*; D066280:Circumventricular Organs / Q000033:anatomy & histology / Q000378:metabolism / Q000473:pathology; D062465:Claudin-3 / Q000378:metabolism*; D006801:Humans; D010870:Pineal Gland / Q000378:metabolism*
chemical_list: C568122:CLDN3 protein, human; D062465:Claudin-3
keywords: Circumventricular organs; Laser microdissection; Microdissection laser; Organes circumventriculaires; Periventricular tumours; Pineal parenchymal tumours; Transcriptome; Tumeur du parenchyme pinéal; Tumeurs périventriculaires