title: Bimetallic Pd-Pt supported graphene promoted enzymatic redox cycling for ultrasensitive electrochemical quantification of microRNA from cell lysates.
abstract: The expression of microRNAs (miRNAs) is related to some cancer diseases. Recently, miRNAs have emerged as new candidate diagnostic and prognostic biomarkers for detecting a wide variety of cancers. Due to low levels, short sequences and high sequence homology among family members, the quantitative miRNA analysis is still a challenge. A novel electrochemical biosensor with triple signal amplification for the ultrasensitive detection of miRNA was developed based on phosphatase, redox-cycling amplification, a bimetallic Pd-Pt supported graphene functionalized screen-printed gold electrode, and two stem-loop structured DNAs as target capturers. The proposed biosensor is highly sensitive due to the enhanced electrochemical signal of Pd-Pt supported graphene and sufficiently selective to discriminate the target miRNA from homologous miRNAs in the presence of loop-stem structure probes with T4 DNA ligase. Therefore, this strategy provided a new and ultrasensitive platform for amplified detection and subsequent analysis of miRNA in biomedical research and clinical diagnosis.
authors: State Key Lab of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P.R.China. jjzhu@nju.edu.cn.
authors: Fang-Fang
authors: FF
authors: Cheng
authors: Jing-Jing
authors: JJ
authors: Zhang
authors: Ting-Ting
authors: TT
authors: He
authors: Jian-Jun
authors: JJ
authors: Shi
authors: E S
authors: ES
authors: Abdel-Halim
authors: Jun-Jie
authors: JJ
authors: Zhu
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D015342:DNA Probes / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D051379:Mice; D035683:MicroRNAs / Q000032:analysis*; D041681:NIH 3T3 Cells; D010084:Oxidation-Reduction; D010165:Palladium / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human; D010984:Platinum; D010165:Palladium; D006046:Gold; D006108:Graphite