title: Changes in store-operated calcium channels in rat bladders with detrusor overactivity.
abstract: OBJECTIVE
  To investigate the regulation of intracellular store-operated calcium channels (SOCCs) in detrusor overactivity (DO) during detrusor function changes in Sprague-Dawley rats.
  
  
  METHODS
  Sixty female Sprague-Dawley rats were randomized into control and DO groups. The contraction of the smooth muscle of the bladder was evaluated in vivo using smooth muscle strips. Changes in intracellular calcium ions were observed using confocal microscopy with preload fluo-4 AM, the SOCC agonist cyclopiazonic acid (CPA; 10 μM) and inhibitor SKF-96365 (10 μM). Cell currents were recorded with the whole-cell patch-clamp technique.
  
  
  RESULTS
  The in vitro frequencies of bladder smooth muscle contraction were significantly different (P <.05) between the DO and control groups, and the amplitudes were not significantly different (P >.05). The changes in intracellular calcium ions and current density were significantly different between the 2 groups (P <.05).
  
  
  CONCLUSIONS
  SOCCs were involved in DO and caused variations in muscle contraction.
authors: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors: Bin
authors: B
authors: Zhao
authors: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors: Xiao
authors: X
authors: Zhong
authors: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors: Xinyu
authors: X
authors: Bai
authors: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors: Qingqing
authors: Q
authors: Wang
authors: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors: Bo
authors: B
authors: Song
authors: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China. Electronic address: lilongk@hotmail.com.
authors: Longkun
authors: L
authors: Li
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D001743:Urinary Bladder / Q000378:metabolism*; D053201:Urinary Bladder, Overactive / Q000378:metabolism*
chemical_list: D015220:Calcium Channels