title: Madecassic acid inhibits the mouse colon cancer growth by inducing apoptosis and immunomodulation.
abstract: OBJECTIVE
  To investigate the antitumor effects of madecassic acid and to investigate the mechanism by which madecassic acid treatment functions in malignancies.
  
  
  METHODS
  Mouse colon CT26 cancer cells injected in mice subcutaneously and intraperitoneally were used to evaluate the tumor growth inhibition by madecassic acid administration. The immunomodulation, cell apoptosis and mitochondrial membrane potential change were evaluated by flow cytometry, cell immunostaining and JC-1 staining, respectively.
  
  
  RESULTS
  Madecassic acid inhibited tumor growth in tumor- bearing mice. CT26 cell apoptosis rate and of the cells from ascites was increased after madecassic acid treatment. Mitochondrial membrane potential in CT26 cells also decreased after madecassic acid treatment. CD4(+) and CD8(+) T- lymphocytes subpopulations increased, while the ratio of CD4(+)/ CD8(+) decreased in after madecassic acid administration.
  
  
  CONCLUSIONS
  Madecassic acid inhibits in vivo CT26 cell-induced tumor growth by facilitating cell apoptosis and increasing immune defense mechanisms.
authors: Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors: Hua
authors: H
authors: Zhang
authors: Ming
authors: M
authors: Zhang
authors: Yun
authors: Y
authors: Tao
authors: Ge
authors: G
authors: Wang
authors: Bing
authors: B
authors: Xia
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D016516:CD4-CD8 Ratio; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D007155:Immunologic Factors / Q000494:pharmacology*; D015847:Interleukin-4 / Q000096:biosynthesis; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D013601:T-Lymphocytes / Q000187:drug effects*; D014315:Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000970:Antineoplastic Agents; D007155:Immunologic Factors; D014315:Triterpenes; D015847:Interleukin-4; C001669:madecassic acid