title: Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin.
abstract: The ultradeformable archaeosomes (UDA, made of total polar archaeolipids (TPA) extracted from the extreme halophile archaea Halorubrum tebenquichense:soybean phosphatidylcholine (SPC):sodium cholate (NaChol), 3:3:1 w:w), are promising topical adjuvants showing high deformability, an essential property for intact skin penetration up to the viable epidermis/dermis. To gain insights on UDA structure, the interactions between TPA, SPC and the edge activator NaChol, were assessed by electrospray ionization mass spectroscopy (ESI-MS) and confocal fluorescence microscopy of giant unilamellar vesicles (GUV). The non covalent heterodimers NaChol-SPC, NaChol-phosphatidylglycerophosphate methyl ether (PGPMe), NaChol-sulfated diglycosyl diphytanyl-glycerol diether (SDGD5) and SPC-PGPMe detected in the gas phase by ESI-MS after direct infusion of UDA, together with the homogeneous partition of FASTDiO and DiIC18 in GUV suggested that in these proportions, lipids and NaChol were miscible. We propose therefore, a model where in UDA the SPC diluted sufficient enough in the rich PGPMe TPA, so as to the low lateral mobility of molecules (typical of rich in PGPMe bilayers) was no longer experienced. We also found that 50μm deep within in vitro human skin canyons, the fluorescence of Alexa fluor 647-ovalbumin in UDL was ∼1.5 folds higher than in UDA, indicating a potential steric hindrance of the voluminous structure of PGPMe UDA bilayer, to the penetration of a particulate cargo such as the 7nm diameter ovalbumin. According to these observations, a further reduction in PGPMe - a lipid playing no immune role - content could help to improve the performance of UDA as topical adjuvants.
authors: Instituto Ferreyra, INIMEC-CONICET, casilla de correo 389, 5000 Cordoba, Argentina.
authors: Dolores C
authors: DC
authors: Carrer
authors: Programa de Nanomedicinas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876 BXD Buenos Aires, Argentina.
authors: Leticia H
authors: LH
authors: Higa
authors: Programa de Nanomedicinas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876 BXD Buenos Aires, Argentina; Unidad Operativa Sistemas de Liberación Controlada, Centro de Investigación y Desarrollo en Química, Instituto Nacional de Tecnología Industrial (INTI), Av. General Paz 5445, B1650WAB Buenos Aires, Argentina.
authors: Maria Victoria Defain
authors: MV
authors: Tesoriero
authors: Programa de Nanomedicinas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876 BXD Buenos Aires, Argentina.
authors: Maria Jose
authors: MJ
authors: Morilla
authors: Programa de Nanomedicinas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876 BXD Buenos Aires, Argentina.
authors: Diana I
authors: DI
authors: Roncaglia
authors: Programa de Nanomedicinas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876 BXD Buenos Aires, Argentina. Electronic address: elromero@unq.edu.ar.
authors: Eder Lilia
authors: EL
authors: Romero
mesh_terms: D000279:Administration, Cutaneous; D000328:Adult; D000818:Animals; D002645:Chickens; D016503:Drug Delivery Systems; D005260:Female; D055352:Halorubrum / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008875:Middle Aged; D010047:Ovalbumin / Q000008:administration & dosage* / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects; D020358:Sodium Cholate / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D053835:Unilamellar Liposomes / Q000737:chemistry
chemical_list: D008055:Lipids; D008081:Liposomes; D053835:Unilamellar Liposomes; D010047:Ovalbumin; D020358:Sodium Cholate
keywords: Penetration; Skin; Ultradeformable archaeosomes