title: Genome sequence of Ensifer sp. TW10; a Tephrosia wallichii (Biyani) microsymbiont native to the Indian Thar Desert.
abstract: Ensifer sp. TW10 is a novel N2-fixing bacterium isolated from a root nodule of the perennial legume Tephrosia wallichii Graham (known locally as Biyani) found in the Great Indian (or Thar) desert, a large arid region in the northwestern part of the Indian subcontinent. Strain TW10 is a Gram-negative, rod shaped, aerobic, motile, non-spore forming, species of root nodule bacteria (RNB) that promiscuously nodulates legumes in Thar Desert alkaline soil. It is fast growing, acid-producing, and tolerates up to 2% NaCl and capable of growth at 40(o)C. In this report we describe for the first time the primary features of this Thar Desert soil saprophyte together with genome sequence information and annotation. The 6,802,256 bp genome has a GC content of 62% and is arranged into 57 scaffolds containing 6,470 protein-coding genes, 73 RNA genes and a single rRNA operon. This genome is one of 100 RNB genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
authors: BNF and Stress Biology Lab, Department of Botany, JNV University, Jodhpur, India.
authors: Nisha
authors: N
authors: Tak
authors: BNF and Stress Biology Lab, Department of Botany, JNV University, Jodhpur, India.
authors: Hukam S
authors: HS
authors: Gehlot
authors: BNF and Stress Biology Lab, Department of Botany, JNV University, Jodhpur, India.
authors: Muskan
authors: M
authors: Kaushik
authors: BNF and Stress Biology Lab, Department of Botany, JNV University, Jodhpur, India.
authors: Sunil
authors: S
authors: Choudhary
authors: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors: Ravi
authors: R
authors: Tiwari
authors: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors: Rui
authors: R
authors: Tian
authors: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors: Yvette
authors: Y
authors: Hill
authors: School of Life and Environmental Sciences, Faculty of Science, Engineering and Built Environment, Deakin University, Melbourne, Victoria, Australia.
authors: Lambert
authors: L
authors: Bräu
authors: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors: Lynne
authors: L
authors: Goodwin
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: James
authors: J
authors: Han
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Konstantinos
authors: K
authors: Liolios
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Marcel
authors: M
authors: Huntemann
authors: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors: Krishna
authors: K
authors: Palaniappan
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Amrita
authors: A
authors: Pati
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Konstantinos
authors: K
authors: Mavromatis
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Natalia
authors: N
authors: Ivanova
authors: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors: Victor
authors: V
authors: Markowitz
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Tanja
authors: T
authors: Woyke
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Nikos
authors: N
authors: Kyrpides
authors: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors: Wayne
authors: W
authors: Reeve
keywords: Alphaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria