title: Kinetics of HIV-1 CTL epitopes recognized by HLA I alleles in HIV-infected individuals at times near primary infection: the Provir/Latitude45 study.
abstract: In patients responding successfully to ART, the next therapeutic step is viral cure. An interesting strategy is antiviral vaccination, particularly involving CD8 T cell epitopes. However, attempts at vaccination are dependent on the immunogenetic background of individuals. The Provir/Latitude 45 project aims to investigate which CTL epitopes in proviral HIV-1 will be recognized by the immune system when HLA alleles are taken into consideration. A prior study (Papuchon et al, PLoS ONE 2013) showed that chronically-infected patients under successful ART exhibited variations of proviral CTL epitopes compared to a reference viral strain (HXB2) and that a generic vaccine may not be efficient. Here, we investigated viral and/or proviral CTL epitopes at different time points in recently infected individuals of the Canadian primary HIV infection cohort and assessed the affinity of these epitopes for HLA alleles during the study period. An analysis of the results confirms that it is not possible to fully predict which epitopes will be recognized by the HLA alleles of the patients if the reference sequences and epitopes are taken as the basis of simulation. Epitopes may be seen to vary in circulating RNA and proviral DNA. Despite this confirmation, the overall variability of the epitopes was low in these patients who are temporally close to primary infection.
authors: University Hospital of Bordeaux and CNRS UMR 5234, Bordeaux, France.
authors: Jennifer
authors: J
authors: Papuchon
authors: University Hospital of Bordeaux and CNRS UMR 5234, Bordeaux, France.
authors: Patricia
authors: P
authors: Pinson
authors: University Hospital of Bordeaux and CNRS UMR 5164, Bordeaux, France.
authors: Gwenda-Line
authors: GL
authors: Guidicelli
authors: University Hospital of Bordeaux and CNRS UMR 5234, Bordeaux, France.
authors: Pantxika
authors: P
authors: Bellecave
authors: Clinique médicale l'Actuel, Montréal, Canada.
authors: Réjean
authors: R
authors: Thomas
authors: Clinique médicale Opus, Montréal, Canada.
authors: Roger
authors: R
authors: LeBlanc
authors: University Hospital of Bordeaux and CNRS UMR 5234, Bordeaux, France.
authors: Sandrine
authors: S
authors: Reigadas
authors: University Hospital of Bordeaux and CNRS UMR 5164, Bordeaux, France.
authors: Jean-Luc
authors: JL
authors: Taupin
authors: Clinique Médicale du Quartier Latin, Montréal, Canada.
authors: Jean Guy
authors: JG
authors: Baril
authors: McGill University Health Centre, Montréal, Canada.
authors: Jean Pierre
authors: JP
authors: Routy
authors: McGill AIDS Centre, Montréal, Canada.
authors: Mark
authors: M
authors: Wainberg
authors: University Hospital of Bordeaux and CNRS UMR 5234, Bordeaux, France.
authors: Hervé
authors: H
authors: Fleury
mesh_terms: D000483:Alleles; D015331:Cohort Studies; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology; D015234:HLA-A Antigens / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D015234:HLA-A Antigens