title: Distinct metabolic pathways trigger adipocyte fat accumulation induced by high-carbohydrate and high-fat diets.
abstract: OBJECTIVE
  Several metabolic pathways may be associated with obesity development and may be differentially modulated by dietary constituents. The aim of this study was to access the adipocyte metabolic pathways that lead to lipid accumulation in fat cells of mice fed a high-carbohydrate or high-fat diet.
  
  
  METHODS
  Male Swiss mice ages 7 to 8 wk were fed a standard laboratory rodent diet-chow diet, a high-carbohydrate (HC) diet, or a high-fat (HF) diet for 8 wk.
  
  
  RESULTS
  Animals fed the HC diet exhibited a high lipogenesis rate and lipoprotein lipase activity even in a fasted state. Peroxisome proliferator-activated receptor gamma and sterol regulatory element-binding proteins-1 mRNAs were increased in adipose tissue. The HF diet did not promote the lipogenic pathways but attenuated lipolytic activity and glucose uptake in adipocytes.
  
  
  CONCLUSIONS
  The HC diet induces constitutive expression of lipogenic transcription factors during fasting, whereas the HF diet decreases lipolysis in adipocytes. Both pathways may contribute to increase fat stores and maintain an obese state.
authors: Department of Nutrition, Nursing School, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: Adaliene@gmail.com.
authors: Adaliene Versiani Matos
authors: AV
authors: Ferreira
authors: Department of Microbiology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Zélia
authors: Z
authors: Menezes-Garcia
authors: Department of Physiology and Biophysics, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Jonas Baeta
authors: JB
authors: Viana
authors: Department of Physiology and Biophysics, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Erica Guilhen
authors: EG
authors: Mário
authors: Department of Physiology and Biophysics, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Leida Maria
authors: LM
authors: Botion
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D004032:Diet / Q000009:adverse effects*; D059305:Diet, High-Fat; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D005215:Fasting; D005947:Glucose / Q000378:metabolism; D050155:Lipogenesis / Q000187:drug effects*; D008066:Lipolysis / Q000187:drug effects*; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D051379:Mice; D009765:Obesity / Q000209:etiology / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D004040:Dietary Carbohydrates; D004041:Dietary Fats; D047495:PPAR gamma; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D014157:Transcription Factors; D008071:Lipoprotein Lipase; D005947:Glucose
keywords: Adipocyte; Carbohydrate; Fat; Lipid accumulation; Metabolic pathway