title: In vivo interaction between the tobacco lectin and the core histone proteins.
abstract: Nictaba, a lectin accumulating in tobacco (Nicotiana tabacum) leaves treated with jasmonate, is considered to act as a signaling protein in the stress physiology of the plant. Immunolocalization studies revealed that Nictaba has a nucleocytoplasmic localization. In previous research, histones were identified as primary interaction partners for Nictaba. Here, the interaction between Nictaba and tobacco histones was scrutinized in vivo. Localization studies, performed in stably transformed Nicotiana benthamiana plants, confirmed the nucleocytoplasmic localization of the lectin and colocalization with the presumed binding partners in the nucleus. Furthermore, bimolecular fluorescence complementation (BiFC) assays confirmed the interaction in vivo. Since BiFC signals were also observed for a Nictaba mutant incapable of binding sugar moieties, this interaction may be mediated by alternative binding sites. The interaction of Nictaba with core histones possibly reflects a role of this stress inducible lectin in gene regulation or chromatin remodeling.
authors: Ghent University, Dept. of Molecular Biotechnology, Lab Biochemistry and Glycobiology, Coupure Links 653, B-9000 Gent, Belgium.
authors: Annelies
authors: A
authors: Delporte
authors: Antwerp University, Dept. of Veterinary Sciences, Lab Cell Biology and Histology, Groenenborgerlaan 171, 2020 Antwerpen, Belgium; Ghent University, Dept. of Molecular Biotechnology, Cell Systems and Imaging Research Group, Coupure Links 653, B-9000 Gent, Belgium; Center for Nano- and Biophotonics, Ghent University, Gent, Belgium.
authors: Winnok H
authors: WH
authors: De Vos
authors: Ghent University, Dept. of Molecular Biotechnology, Lab Biochemistry and Glycobiology, Coupure Links 653, B-9000 Gent, Belgium; Center for Nano- and Biophotonics, Ghent University, Gent, Belgium. Electronic address: elsjm.vandamme@ugent.be.
authors: Els J M
authors: EJ
authors: Van Damme
mesh_terms: D001665:Binding Sites; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D031541:Fluorescence Resonance Energy Transfer; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D037102:Lectins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D054883:Oxylipins / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D006657:Histones; D037102:Lectins; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C011006:jasmonic acid
keywords: BiFC; FRET; Histone; Interaction; Localization; Nictaba