title: Intermedin inhibits uptake of oxidized LDL via CD36 pathway in RAW264.7 cells.
abstract: Intermedin (IMD) exerts a potent function in preventing atherosclerosis, while the mechanism remains unclear. Here we investigated the potential molecular mechanism responsible for the protective function of IMD in preventing foam cell formation in RAW264.7 cells. In our present study, IMD significantly inhibited intracellular cholesterol accumulation. Additionally, IMD dose-dependently down-regulated CD36 expression, which was confirmed by real-time quantitative reverse transcription-PCR and Western blot analysis. Our data suggest that IMD could inhibit the formation of foam cells through, at least partly, a CD36-dependent mechanism. This study suggests that IMD may be a therapeutic candidate for treating atherosclerosis.
authors: Yong
authors: Y
authors: Wang
authors: Rui
authors: R
authors: Yang
authors: Xiaoni
authors: X
authors: Chen
authors: Xin
authors: X
authors: Zhang
authors: Sen
authors: S
authors: He
authors: Jiayue
authors: J
authors: Feng
authors: Shixi
authors: S
authors: Wan
authors: Si
authors: S
authors: Wang
authors: Xiaoping
authors: X
authors: Chen
mesh_terms: D000818:Animals; D001391:Azo Compounds; D015153:Blotting, Western; D018955:CD36 Antigens / Q000096:biosynthesis / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D008077:Lipoproteins, LDL / Q000378:metabolism*; D051379:Mice; D010084:Oxidation-Reduction; D036361:Peptide Hormones / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: C484330:ADM2 protein, human; D001391:Azo Compounds; D018955:CD36 Antigens; D017931:DNA Primers; D008077:Lipoproteins, LDL; D036361:Peptide Hormones; C011049:oil red O