title: Triterpenoid saponins from the roots of Clematis argentilucida.
abstract: Reinvestigation of the n-BuOH extract of the roots of Clematis argentilucida led to the isolation of a new ursane-type triterpenoid saponin 1 and a new taraxerane-type saponin 2, four known saponins 3-6 first isolated from the species, together with seven saponins 7-13 reported in the previous papers. The structures of saponins 1-6 were elucidated by extensive spectroscopic analysis and chemical evidences. The ursane-type and taraxerane-type triterpenoid saponins were obtained from genus Clematis for the first time, and the aglycone of saponin 1, 3β,28-dihydroxy-18αH-ursan-20-en was first encountered. The cytotoxicity of all the saponins was evaluated against human glioblastoma U251MG cell lines. The monodesmosidic saponins 1, 2 and 4-8 exhibited cytotoxic activity against the cells with IC50 values ranging from 6.95 to 38.51 μM.
authors: Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China; Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China.
authors: Mei
authors: M
authors: Zhao
authors: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
authors: Ning
authors: N
authors: Ma
authors: School of Traditional Chinese Medicines, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
authors: Feng
authors: F
authors: Qiu
authors: Research & Development Center of Biorational Pesticide, College of Plant Protection, Northwest A&F University, Yangling 712100, PR China.
authors: Xiangrong
authors: X
authors: Tian
authors: Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China.
authors: Yan
authors: Y
authors: Zhang
authors: Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China; Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China. Electronic address: tanghaifeng71@163.com.
authors: Haifeng
authors: H
authors: Tang
authors: Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China. Electronic address: lxylxywy@163.com.
authors: Xinyou
authors: X
authors: Liu
mesh_terms: D045744:Cell Line, Tumor; D039324:Clematis / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D012503:Saponins
keywords: Clematis argentilucida; Cytotoxicity; Ranunculaceae; Taraxerane-type; Triterpenoid saponin; Ursane-type