title: Differentiation induction of mouse cardiac stem cells into sinus node-like cells by co-culturing with sinus node.
abstract: Sinus nodal cells can generate a diastolic or "pacemaker" depolarization at the end of an action potential driving the membrane potential slowly up to the threshold for firing the next action potential. It has been proved that adult cardiac stem cells (CSCs) can differentiate into sinus nodal cells by demethylating agent. However, there is no report about adult CSCs-derived sinus nodal cells with pacemaker current (the funny current, I f). In this study, we isolated the mouse adult CSCs from mouse hearts by the method of tissue explants adherence. The expression of c-kit protein indicated the isolation of CSCs. Then we co-cultured mouse CSCs with mouse sinus node tissue to induce the differentiation of these CSCs into sinus node-like cells, which was proved by identifying the enhanced expression of marker proteins cTnI, cTnT and α-Actinin with Immunofluorescence staining. At the same time, with whole-cell patch-clamp we detected the I f current, which can be blocked by CsCl, in these differentiated cells. In conclusion, by confirming specific I f current in the induced node-like cells, our work shows a method inducing differentiation of CSCs into sinus node-like cells, which can provide helpful information for the further research on sick sinus syndrome.
authors: Department of Cardiothoracic, The Affiliated Hospital of Luzhou Medical College Sichuan, P. R. China.
authors: Yi-Bing
authors: YB
authors: Fang
authors: Department of Cardiothoracic, The Affiliated Hospital of Luzhou Medical College Sichuan, P. R. China.
authors: Xuan
authors: X
authors: Liu
authors: Department of Electrophysiology, Institute of Cardiovasology, Luzhou Medical College Sichuan, P. R. China.
authors: Jing
authors: J
authors: Wen
authors: Department of Cardiothoracic, The Affiliated Hospital of Luzhou Medical College Sichuan, P. R. China.
authors: Xiao-Jun
authors: XJ
authors: Tang
authors: Department of Cardiothoracic, The Affiliated Hospital of Luzhou Medical College Sichuan, P. R. China.
authors: Feng-Xu
authors: FX
authors: Yu
authors: Department of Cardiothoracic, The Affiliated Hospital of Luzhou Medical College Sichuan, P. R. China.
authors: Ming-Bin
authors: MB
authors: Deng
authors: Department of Cardiothoracic, The Affiliated Hospital of Luzhou Medical College Sichuan, P. R. China.
authors: Chang-Xue
authors: CX
authors: Wu
authors: Department of Cardiothoracic, The Affiliated Hospital of Luzhou Medical College Sichuan, P. R. China.
authors: Bin
authors: B
authors: Liao
mesh_terms: D000185:Actinin / Q000378:metabolism; D000818:Animals; D001683:Biological Clocks; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D018920:Coculture Techniques; D008564:Membrane Potentials; D051379:Mice; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D012849:Sinoatrial Node / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors; D046509:Tissue Culture Techniques; D019210:Troponin I / Q000378:metabolism; D020107:Troponin T / Q000378:metabolism
chemical_list: D015415:Biomarkers; D019210:Troponin I; D020107:Troponin T; D000185:Actinin; D019009:Proto-Oncogene Proteins c-kit
keywords: Cardiac stem cells; If current; co-culture; sinus node