title: [Soluble expression and activity evaluation of SDF-1/54R, a specific antagonist of CXCR7].
abstract: OBJECTIVE
  To construct a soluble prokaryotic expression vector of the CXCR7-specific antagonist SDF-1/54R and evaluate its activity.
  
  
  METHODS
  SDF-1/54r gene amplified by PCR was inserted into the soluble expression vector pET-41a+ engineered with GST fusion tag, and the recombinant vector was transformed into E. coli strain BL21 (DE3). After IPTG induction of E. coli, the expressed recombinant protein was purified with GST affinity chromatography purification system and confirmed by SDS-PAGE and Western blotting assay. The target protein SDF-1/54R was obtained after digestion of the purified product with enterokinase. Breast cancer MCF-7 cells with high expression of CXCR7 was treated with SDF-1/54R and the cell proliferation and metastasis was evaluated with MTT and chemotaxis assays.
  
  
  RESULTS
  The target protein SDF-1/54R obtained showed an obvious inhibitory effect on the proliferation and metastasis of MCF-7 cells as confirmed by MTT and chemotaxis assays.
  
  
  CONCLUSIONS
  SDF-1/54R is a good antagonist of CXCR7 and shows a potential value as an effective anti-cancer agent.
authors: Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.E-mail: yzcao1314@126.com.
authors: Yuanzhi
authors: Y
authors: Cao
authors: Feihua
authors: F
authors: Yang
authors: Weifeng
authors: W
authors: Ma
mesh_terms: D015153:Blotting, Western; D054377:Chemokine CXCL12 / Q000378:metabolism*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005822:Genetic Vectors; D006801:Humans; D016133:Polymerase Chain Reaction; D054387:Receptors, CXCR / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: C501896:ACKR3 protein, human; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D054387:Receptors, CXCR; D011994:Recombinant Proteins