title: Reversible mitochondrial DNA accumulation in nuclei of pluripotent stem cells.
abstract: According to the endosymbiotic hypothesis, the precursor of mitochondria invaded the precursor of eukaryotic cells, a process that began roughly 2 billion years ago. Since then, the majority of the genetic material translocated from the mitochondria to the nucleus, where now almost all mitochondrial proteins are expressed. Only a tiny amount of DNA remained in the mitochondria, known as mitochondrial DNA (mtDNA). In this study, we report that the transfer of mtDNA fragments to the nucleus of pluripotent stem cells is still ongoing. We show by in situ hybridization and agarose DNA two-dimensional gel technique that induced pluripotent stem (iPS) cells contain high levels of mtDNA in the nucleus. We found that a large proportion of the accumulated mtDNA sequences appear to be extrachromosomal. Accumulation of mtDNA in the nucleus is present not only in the iPS cells, but also in embryonic stem (ES) cells. However upon differentiation, the level of mtDNA in the nuclei of iPS and ES cells is substantially reduced. This reversible accumulation of mtDNA in the nucleus supports the notion that the nuclear copy number of mtDNA sequences may provide a novel mechanism by which chromosomal DNA is dynamically regulated in pluripotent stem cells.
authors: 1 Department of Cell Biology and Molecular Medicine, Rutgers Biomedical and Health Sciences , Newark, New Jersey.
authors: Joel S
authors: JS
authors: Schneider
authors: Xin
authors: X
authors: Cheng
authors: Qingshi
authors: Q
authors: Zhao
authors: Chingiz
authors: C
authors: Underbayev
authors: J Patrick
authors: JP
authors: Gonzalez
authors: Elizabeth S
authors: ES
authors: Raveche
authors: Diego
authors: D
authors: Fraidenraich
authors: Andreas S
authors: AS
authors: Ivessa
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002875:Chromosomes / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000378:metabolism; D018628:Gene Dosage / Q000235:genetics; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism*
chemical_list: D004272:DNA, Mitochondrial