title: Baicalin, a metabolite of baicalein with antiviral activity against dengue virus.
abstract: Baicalin, a flavonoid derived from Scutellaria baicalensis, is the main metabolite of baicalein released following administration in different animal models and human. We previously reported the antiviral activity of baicalein against dengue virus (DENV). Here, we examined the anti-DENV properties of baicalin in vitro, and described the inhibitory potentials of baicalin at different steps of DENV-2 (NGC strain) replication. Our in vitro antiviral experiments showed that baicalin inhibited virus replication at IC50 = 13.5 ± 0.08 μg/ml with SI = 21.5 following virus internalization by Vero cells. Baicalin exhibited virucidal activity against DENV-2 extracellular particles at IC50 = 8.74 ± 0.08 μg/ml and showed anti-adsorption effect with IC50 = 18.07 ± 0.2 μg/ml. Our findings showed that baicalin as the main metabolite of baicalein exerting in vitro anti-DENV activity. Further investigations on baicalein and baicalin to deduce its antiviral therapeutic effects are warranted.
authors: Tropical Infectious Disease Research and Education Center, Department of Medical Microbiology Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Ehsan
authors: E
authors: Moghaddam
authors: Tropical Infectious Disease Research and Education Center, Department of Medical Microbiology Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Boon-Teong
authors: BT
authors: Teoh
authors: Tropical Infectious Disease Research and Education Center, Department of Medical Microbiology Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Sing-Sin
authors: SS
authors: Sam
authors: Tropical Infectious Disease Research and Education Center, Department of Medical Microbiology Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Rafidah
authors: R
authors: Lani
authors: Tropical Infectious Disease Research and Education Center, Department of Medical Microbiology Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Pouya
authors: P
authors: Hassandarvish
authors: Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Zamri
authors: Z
authors: Chik
authors: Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli 350, Taiwan, ROC.
authors: Andrew
authors: A
authors: Yueh
authors: Tropical Infectious Disease Research and Education Center, Department of Medical Microbiology Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Sazaly
authors: S
authors: Abubakar
authors: Tropical Infectious Disease Research and Education Center, Department of Medical Microbiology Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Keivan
authors: K
authors: Zandi
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D003716:Dengue Virus / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D044950:Flavanones / Q000378:metabolism; D005419:Flavonoids / Q000008:administration & dosage* / Q000378:metabolism; D006801:Humans; D007928:Lethal Dose 50; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology*
chemical_list: D000998:Antiviral Agents; D044950:Flavanones; D005419:Flavonoids; C038044:baicalin; C006680:baicalein