title: Bacterial contamination of kale (Brassica oleracea Acephala) along the supply chain in Nairobi and its environment.
abstract: OBJECTIVE
  To assess the microbiological safety of kale (Brassica oleracea Acephala) produced from farms and those sold at the markets with special focus on coliforms, E.coli and Salmonella.
  
  
  METHODS
  A cross sectional study.
  
  
  METHODS
  Peri-Urban farms (in Athi River, Ngong and Wangige), wet markets (in Kawangware, Kangemi and Githurai), supermarkets and high-end specialty store both within Nairobi city.
  
  
  RESULTS
  Mean coliform count on vegetables from farms were 2.6 x 10(5) +/- 5.0 x 10(5) cfu/g while those from the wet markets were 4.6 x 10(6) +/- 9.1 x 10(6) cfu/g, supermarkets, 2.6 x 10(6) +/- 2.7 x 10(6) and high-end specialty store 4.7 x 10(5) +/- 8.9 x 10 (5). Coliform numbers obtained on kales from the wet markets and supermarkets were significantly higher (p < 0.05) compared to those from farms, while kale samples purchased from high-end specialty store had similar levels of coliform loads as those from the farms. E. coli prevalence in the wet markets, supermarkets and high-end specialty store were: 40, 20 and 20%, respectively. Salmonella was detected on 4.5 and 6.3% of samples collected from the farms in Wangige and wet market in Kawangware, respectively. Fecal coliforms in water used on farms (for irrigation) and in the markets (for washing the vegetables) exceeded levels recommended by World Health Organization (WHO) of 10(3) organisms per 100 milliliter while Salmonella was detected in 12.5% of washing water samples collected from Kangemi market.
  
  
  CONCLUSIONS
  Poor cultivation practices and poor handling of vegetables along the supply chain could increase the risk of pathogen contamination thus puting the health of the public at risk, therefore good agricultural and handling practices should be observed.
authors: E K
authors: EK
authors: Kutto
authors: M W
authors: MW
authors: Ngigi
authors: N
authors: N
authors: Karanja
authors: E
authors: E
authors: Kange'the
authors: L C
authors: LC
authors: Bebora
authors: C J
authors: CJ
authors: Lagerkvist
authors: P G
authors: PG
authors: Mbuthia
authors: L W
authors: LW
authors: Njagi
authors: J J
authors: JJ
authors: Okello
mesh_terms: D057914:Agricultural Irrigation; D001937:Brassica / Q000382:microbiology*; D003132:Commerce; D004926:Escherichia coli / Q000302:isolation & purification*; D005516:Food Microbiology; D007630:Kenya; D012475:Salmonella / Q000302:isolation & purification*; D014871:Water Microbiology