title: Effectiveness of antimicrobial peptide immobilization for preventing perioperative cornea implant-associated bacterial infection.
abstract: Titanium (Ti) is a promising candidate biomaterial for an artificial corneal skirt. Antimicrobial peptide (AMP) immobilization may improve the bactericidal effect of the Ti substrate. In this study, we tested the bactericidal efficacy of a functionalized Ti surface in a rabbit keratitis model. A corneal stromal pocket was created by a femtosecond laser. The Ti films were then inserted into the pocket, and Staphylococcus aureus or Pseudomonas aeruginosa was inoculated into the pocket above the implant films. The corneas with Ti-AMP implants were compared with the corneas implanted with unprotected Ti by slit lamp observation and anterior segment optical coherence tomography (AS-OCT). Inflammatory responses were evaluated by bacterium counting, hematoxylin-eosin staining, and immunostaining. There was a lower incidence and a lesser extent of infection on rabbit corneas with Ti-AMP implants than on those with unprotected Ti implants. The bactericidal effect of AMP against S. aureus was comparable to that of postoperative prophylactic antibiotic treatment; hence, SESB2V AMP bound to the Ti implant provided functional activity in vivo, but its efficacy was greater against S. aureus than against P. aeruginosa. This work suggests that SESB2V AMP can be successfully functionalized in a rabbit keratitis model to prevent perioperative corneal infection.
authors: Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore.
authors: Xiao Wei
authors: XW
authors: Tan
authors: Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore.
authors: Tze Wei
authors: TW
authors: Goh
authors: Antimicrobials Group, Singapore Eye Research Institute, Singapore.
authors: P
authors: P
authors: Saraswathi
authors: Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore.
authors: Chan Lwin
authors: CL
authors: Nyein
authors: Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore.
authors: Melina
authors: M
authors: Setiawan
authors: Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore.
authors: Andri
authors: A
authors: Riau
authors: Antimicrobials Group, Singapore Eye Research Institute, Singapore Duke-NUS Graduate Medical School, Singapore.
authors: R
authors: R
authors: Lakshminarayanan
authors: Antimicrobials Group, Singapore Eye Research Institute, Singapore Duke-NUS Graduate Medical School, Singapore.
authors: Shouping
authors: S
authors: Liu
authors: Yong Loo Lin School of Medicine, National University of Singapore, Singapore Singapore National Eye Centre, Singapore Duke-NUS Graduate Medical School, Singapore.
authors: Donald
authors: D
authors: Tan
authors: Antimicrobials Group, Singapore Eye Research Institute, Singapore Yong Loo Lin School of Medicine, National University of Singapore, Singapore Duke-NUS SRP Neuroscience and Behavioral Disorders, Singapore.
authors: Roger W
authors: RW
authors: Beuerman
authors: Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore Antimicrobials Group, Singapore Eye Research Institute, Singapore Singapore National Eye Centre, Singapore Duke-NUS Graduate Medical School, Singapore jodmehta@gmail.com.
authors: Jodhbir S
authors: JS
authors: Mehta
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage* / Q000627:therapeutic use; D016039:Corneal Transplantation / Q000009:adverse effects*; D011552:Pseudomonas Infections / Q000517:prevention & control*; D011817:Rabbits; D066167:Slit Lamp; D013203:Staphylococcal Infections / Q000517:prevention & control*; D013530:Surgical Wound Infection / Q000517:prevention & control*; D014025:Titanium; D041623:Tomography, Optical Coherence
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D014025:Titanium