title: Relationship between tumor necrosis factor-α release and granulocyte and monocyte adsorption to cellulose acetate beads.
abstract: Tumor necrosis factor-α, (TNF)-α, a proinflammatory cytokine, is produced by activated granulocytes and monocytes (GMs) and implicated as a major factor in inflammatory bowel disease (IBD) pathogenesis. Reduction of TNF-α should improve IBD pathology. GM adsorptive apheresis (GMA) is an effective therapy for inflammatory disorders including IBD. GM adsorption to cellulose acetate (CA) beads induces anti-inflammatory cytokine release, although these effects on TNF-α release are not clarified. We hypothesized that GMA may inhibit TNF-α release. The aim of the present study was to clarify the effects of GM adsorption to CA beads on TNF-α release in vitro. Peripheral blood was incubated with and without CA beads and TNF-α was measured. For comparison, TNF-α was measured in another lipopolysaccharide (LPS)-containing peripheral blood sample incubated similarly. The amount of TNF-α in blood samples incubated with CA beads was significantly higher than in those incubated without beads, although it was significantly lower than TNF-α incubated with LPS-containing sample without beads. The amount of TNF-α after incubation with CA beads positively correlated with GM adsorption ratio. GM adsorption to CA beads induced a small amount of TNF-α release. This is the first report on TNF-α release induced via GM adsorption stimuli. The biological effects of TNF-α release during GM adsorption need to be clarified.
authors: Department of Gastroenterology, Yamagata University Faculty of Medicine, Yamagata, Japan.
authors: Shoichi
authors: S
authors: Nishise
authors: Yasuhiko
authors: Y
authors: Abe
authors: Eiki
authors: E
authors: Nomura
authors: Takeshi
authors: T
authors: Sato
authors: Yu
authors: Y
authors: Sasaki
authors: Daisuke
authors: D
authors: Iwano
authors: Kazuya
authors: K
authors: Yoshizawa
authors: Makoto
authors: M
authors: Yagi
authors: Yuko
authors: Y
authors: Nishise
authors: Yoshiyuki
authors: Y
authors: Ueno
mesh_terms: D000327:Adsorption; D001781:Blood Component Removal / Q000379:methods*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009000:Monocytes / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; C005062:acetylcellulose; D002482:Cellulose
keywords: Adsorption; Apheresis; Granulocyte; Inflammatory bowel disease; Monocyte; Tumor necrosis factor-α