title: CRISPLD2 is a target of progesterone receptor and its expression is decreased in women with endometriosis.
abstract: Endometriosis, defined as the presence of endometrial cells outside of the uterine cavity, is a major cause of infertility and pelvic pain, afflicting more than 10% of reproductive age women. Endometriosis is a chronic inflammatory disease and lipopolysaccharide promotes the proliferation and invasion of endometriotic stromal cells. Cysteine-rich secretory protein LCCL domain-containing 2 (CRISPLD2) has high affinity for lipopolysaccharide and plays a critical role in defense against endotoxin shock. However, the function of CRISPLD2 has not been studied in endometriosis and uterine biology. Herein, we examined the expression of CRISPLD2 in endometrium from patients with and without endometriosis using immunohistochemistry. The expression of CRISPLD2 was higher in the secretory phase in human menstrual cycle compared to proliferative phase. The expression of CRISPLD2 was significantly decreased in the endometrium of women with endometriosis in the early secretory phase compared to women without endometriosis. The increase of CRISPLD2 expression at the early secretory and dysregulation of its expression in endometriosis suggest progesterone (P4) regulation of CRISPLD2. To investigate whether CRISPLD2 is regulated by P4, we examined the expression of the CRISPLD2 in the uteri of wild-type and progesterone receptor knock out (PRKO) mice. The expression of CRISPLD2 was significantly increased after P4 treatment in the wild-type mice. However, CRISPLD2 expression was significantly decreased in the (PRKO) mice treated with P4. During early pregnancy, the expression of CRISPLD2 was increased in decidua of implantation and post-implantation stages. CRISPLD2 levels were also increased in cultured human endometrial stromal cells during in vitro decidualization. These results suggest that the CRISPLD2 is a target of the progesterone receptor and may play an important role in pathogenesis of endometriosis.
authors: Department of Obstetrics, Gynecology & Reproductive Biology, Michigan State University, College of Human Medicine, Grand Rapids, Michigan, United States of America.
authors: Jung-Yoon
authors: JY
authors: Yoo
authors: Department of Obstetrics, Gynecology & Reproductive Biology, Michigan State University, College of Human Medicine, Grand Rapids, Michigan, United States of America; Department of Biotechnology and Bioinformatics, Korea University, Sejong, South Korea.
authors: Heesung
authors: H
authors: Shin
authors: Department of Obstetrics, Gynecology & Reproductive Biology, Michigan State University, College of Human Medicine, Grand Rapids, Michigan, United States of America.
authors: Tae Hoon
authors: TH
authors: Kim
authors: Department of Food Science and Technology, Korea National University of Transportation, Chungbuk, South Korea.
authors: Won-Seok
authors: WS
authors: Choi
authors: Department of Obstetrics, Gynecology & Reproductive Biology, Michigan State University, College of Human Medicine, Grand Rapids, Michigan, United States of America.
authors: Susan D
authors: SD
authors: Ferguson
authors: Department of Obstetrics, Gynecology & Reproductive Biology, Michigan State University, College of Human Medicine, Grand Rapids, Michigan, United States of America.
authors: Asgerally T
authors: AT
authors: Fazleabas
authors: Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill, North Carolina, United States of America.
authors: Steven L
authors: SL
authors: Young
authors: Department of Obstetrics and Gynecology, University Medical Group, Greenville Hospital System, Greenville, South Carolina, United States of America.
authors: Bruce A
authors: BA
authors: Lessey
authors: Department of Biotechnology and Bioinformatics, Korea University, Sejong, South Korea.
authors: Un-Hwan
authors: UH
authors: Ha
authors: Department of Obstetrics, Gynecology & Reproductive Biology, Michigan State University, College of Human Medicine, Grand Rapids, Michigan, United States of America.
authors: Jae-Wook
authors: JW
authors: Jeong
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D050835:Interferon Regulatory Factors / Q000235:genetics / Q000378:metabolism*; D008597:Menstrual Cycle / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008875:Middle Aged; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011980:Receptors, Progesterone / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014599:Uterus / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
chemical_list: C525599:CRISPLD2 protein, human; D015815:Cell Adhesion Molecules; D050835:Interferon Regulatory Factors; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D011374:Progesterone