title: Effect of solution and post-mortem time on mechanical and histological properties of liver during cold preservation.
abstract: BACKGROUND
  In liver transplantation, the donor and recipient are in different locations most of the time, and longer preservation periods are inevitable. Hence, the choice of the preservation solution and the duration of the preservation period are critical for the success of the transplant surgery.
  
  
  OBJECTIVE
  In this study, we examine the mechanical and histological properties of the bovine liver tissue stored in Lactated Ringer's (control), HTK and UW solutions as a function of preservation period.
  
  
  METHODS
  The mechanical experiments are conducted with a shear rheometer on cylindrical tissue samples extracted from 3 bovine livers and the change in viscoelastic material properties of the bovine liver is characterized using the fractional derivative Kelvin-Voigt Model. Also, the histological examinations are performed on the same liver samples under a light microscope.
  
  
  RESULTS
  The results show that the preservation solution and period have a significant effect on the mechanical and histological properties of the liver tissue. The storage and loss shear moduli, the number of the apoptotic cells, the collagen accumulation, and the sinusoidal dilatation increase, and the glycogen deposition decreases as the preservation period is longer.
  
  
  CONCLUSIONS
  Based on the statistical analyses, we observe that the liver tissue is preserved well in all three solutions for up to 11 h. After then, UW solution provides a better preservation up to 29 h. However, for preservation periods longer than 29 h, HTK is a more effective preservation solution based on the least amount of change in mechanical properties. On the other hand, the highest correlation between the mechanical and histological properties is observed for the liver samples preserved in UW solution.
authors: College of Engineering, Koc University, Istanbul, Turkey.
authors: Mehmet
authors: M
authors: Ayyildiz
authors: School of Medicine, Koc University, Istanbul, Turkey.
authors: Ranan Gulhan
authors: RG
authors: Aktas
authors: College of Engineering, Koc University, Istanbul, Turkey.
authors: Cagatay
authors: C
authors: Basdogan
mesh_terms: D000818:Animals; D002417:Cattle; D008099:Liver / Q000473:pathology*; D016031:Liver Transplantation / Q000379:methods*; D011309:Preservation, Biological; D012212:Rheology; D012996:Solutions
chemical_list: D012996:Solutions
keywords: Liver; histology; mechanical characterization; preservation; rheology; transplantation