title: Involvement of IL-17A in preventing the development of deep-seated candidiasis from oropharyngeal infection.
abstract: In this study we show that corticosteroid-treated Il17a(-/-) mice develop invasive candidiasis from oropharyngeal infection whereas WT mice do not. By using an established murine model of oral candidiasis we document the spatial and temporal progression of fungal infection. The histological analysis of tissues in Il17a(-/-) mice showed massive infiltration of the fungus in the stomach and alterations of the gastrointestinal tract segments. Both increased permeability and mucosal ulcerations of the intestinal barrier are seen to favor Candida albicans dissemination which was quantified both in kidney and liver where typical candidal abscesses were detected. Neutrophils from Il17a(-/-) were as capable of phagocytosing the fungus comparable to that of WT mice, however, they showed decreased candidacidal ability. Our data implies that IL-17A is crucial for preventing the passage from mucosal to disseminated candidiasis. As such, our model may be suitable to study the mechanisms favoring C. albicans translocation to internal organs.
authors: Internal Medicine Section, Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
authors: Paolo
authors: P
authors: Mosci
authors: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors: Elena
authors: E
authors: Gabrielli
authors: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors: Eugenio
authors: E
authors: Luciano
authors: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors: Stefano
authors: S
authors: Perito
authors: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors: Antonio
authors: A
authors: Cassone
authors: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors: Eva
authors: E
authors: Pericolini
authors: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy. Electronic address: anna.vecchiarelli@unipg.it.
authors: Anna
authors: A
authors: Vecchiarelli
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000276:immunology*; D058387:Candidemia / Q000276:immunology*; D002180:Candidiasis, Oral / Q000150:complications* / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology / Q000473:pathology; D020381:Interleukin-17 / Q000172:deficiency / Q000276:immunology*; D008100:Liver Abscess / Q000382:microbiology / Q000473:pathology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D020381:Interleukin-17
keywords: Bioluminescence; Candida albicans; Imaging; Longitudinal study; OPC; Real-time