title: Maleimidyl magnetic nanoplatform for facile molecular MRI.
abstract: In this study, we developed the maleimidyl magnetic nanoplatform, which enables functional targeting of a biomarker-specific moiety for molecular imaging via MRI. The maleimide group of the maleimidyl magnetic nanoplatform is conjugated with a thiol group without additional crosslinkers and side products. A physicochemical analysis was conducted to verify the effectiveness of the maleimidyl magnetic nanoplatform, and the existence of the maleimidyl group was investigated using the platform. To prepare biomarker-specific MRI probes, a thiolated aptamer and peptide were immobilized onto the maleimidyl group of the maleimidyl magnetic nanoplatform. The fabricated MRI probes were applied to four cancer cell lines: HT1080, MCF7, MKN45, and HEK293T. To investigate the potential of the molecular MRI probe, the target-biomarker specificity was confirmed without serious cytotoxicity, and in vivo MRI analysis using a xenograft mouse model was demonstrated. We believe these results will be useful for fabricating molecular MRI probes for the diagnosis of cancer.
authors: Department of Radiology, Yonsei University College of Medicine, Seoul, 120-752, Republic of Korea. Nanomedical National Core Research Center, Yonsei University, Seoul, 120-749, Republic of Korea.
authors: Dan
authors: D
authors: Heo
authors: Eugene
authors: E
authors: Lee
authors: Minhee
authors: M
authors: Ku
authors: Seungyeon
authors: S
authors: Hwang
authors: Bongjune
authors: B
authors: Kim
authors: Yeonji
authors: Y
authors: Park
authors: Young
authors: Y
authors: Han Lee
authors: Yong-Min
authors: YM
authors: Huh
authors: Seungjoo
authors: S
authors: Haam
authors: Jae-Ho
authors: JH
authors: Cheong
authors: Jaemoon
authors: J
authors: Yang
authors: Jin-Suck
authors: JS
authors: Suh
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D061986:MCF-7 Cells; D060328:Magnetic Phenomena; D008279:Magnetic Resonance Imaging / Q000295:instrumentation*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D008301:Maleimides / Q000138:chemical synthesis* / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D036103:Nanotechnology / Q000295:instrumentation*; D009369:Neoplasms / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D058185:Magnetite Nanoparticles; D008301:Maleimides