title: Stable isotope labeling by amino acids in cell culture (SILAC) for quantitative proteomics.
abstract: Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful approach for high-throughput quantitative proteomics. SILAC allows highly accurate protein quantitation through metabolic encoding of whole cell proteomes using stable isotope labeled amino acids. Since its introduction in 2002, SILAC has become increasingly popular. In this chapter we review the methodology and application of SILAC, with an emphasis on three research areas: dynamics of posttranslational modifications, protein-protein interactions, and protein turnover.
authors: Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 540 First Avenue, New York, NY, 10016, USA.
authors: Esthelle
authors: E
authors: Hoedt
authors: Guoan
authors: G
authors: Zhang
authors: Thomas A
authors: TA
authors: Neubert
mesh_terms: D018929:Cell Culture Techniques; D007553:Isotope Labeling / Q000379:methods*; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome