title: An unnatural amino acid based fluorescent probe for phenylalanine ammonia lyase.
abstract: A fluorescent probe (2a-LP) based on an unnatural amino acid (UAA) is developed for the detection of phenylalanine ammonia lyase (PAL). In the presence of PAL, 2a-LP is catalytically deaminated to ortho-amino-transcinnamic acid (o-a-CA), which shows a remarkable “off–on” fluorescence signal. Thus, the probe 2a-LP enables direct visualization of the PAL activity in tomato under UV illumination and has potential in vitro assays.
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, PR China. wpzhu@ecust.edu.cn yfxu@ecust.edu.cn.
authors: Zhenlin
authors: Z
authors: Tian
authors: Weiping
authors: W
authors: Zhu
authors: Yufang
authors: Y
authors: Xu
authors: Xuhong
authors: X
authors: Qian
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D055162:Biocatalysis; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D018551:Solanum lycopersicum / Q000201:enzymology; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D000596:Amino Acids; D005456:Fluorescent Dyes; D010650:Phenylalanine Ammonia-Lyase