title: Advanced silk material spun by a transgenic silkworm promotes cell proliferation for biomedical application.
abstract: Natural silk fiber spun by the silkworm Bombyx mori is widely used not only for textile materials, but also for biofunctional materials. In the present study, we genetically engineered an advanced silk material, named hSFSV, using a transgenic silkworm, in which the recombinant human acidic fibroblast growth factor (hFGF1) protein was specifically synthesized in the middle silk gland and secreted into the sericin layer to surround the silk fiber using our previously optimized sericin1 expression system. The content of the recombinant hFGF1 in the hSFSV silk was estimated to be approximate 0.07% of the cocoon shell weight. The mechanical properties of hSFSV raw silk fiber were enhanced slightly compared to those of the wild-type raw silk fiber, probably due to the presence of the recombinant of hFGF1 in the sericin layer. Remarkably, the hSFSV raw silk significantly stimulated the cell growth and proliferation of NIH/3T3 mouse embryonic fibroblast cells, suggesting that the mitogenic activity of recombinant hFGF1 was well maintained and functioned in the sericin layer of hSFSV raw silk. These results show that the genetically engineered raw silk hSFSV could be used directly as a fine biomedical material for mass application. In addition, the strategy whereby functional recombinant proteins are expressed in the sericin layer of silk might be used to create more genetically engineered silks with various biofunctions and applications.
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China.
authors: Feng
authors: F
authors: Wang
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China.
authors: Hanfu
authors: H
authors: Xu
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China.
authors: Yuancheng
authors: Y
authors: Wang
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China.
authors: Riyuan
authors: R
authors: Wang
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China.
authors: Lin
authors: L
authors: Yuan
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China.
authors: Huan
authors: H
authors: Ding
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China.
authors: Chunnuan
authors: C
authors: Song
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China.
authors: Sanyuan
authors: S
authors: Ma
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China.
authors: Zhixin
authors: Z
authors: Peng
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China.
authors: Zhangchuan
authors: Z
authors: Peng
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China.
authors: Ping
authors: P
authors: Zhao
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China; College of Biotechnology, Southwest University, Chongqing 400715, People's Republic of China. Electronic address: xiaqy@swu.edu.cn.
authors: Qingyou
authors: Q
authors: Xia
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001672:Biocompatible Materials / Q000737:chemistry*; D012831:Bombyx / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D016220:Fibroblast Growth Factor 1 / Q000235:genetics / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000378:metabolism; D047011:Silk / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D011994:Recombinant Proteins; D047011:Silk; D016220:Fibroblast Growth Factor 1
keywords: Biomedical material; Recombinant human acidic fibroblast growth factor; Silk; Transgenic silkworm