title: Expression of urokinase type plasminogen activator receptor (uPAR) in the bovine oviduct: Relationship with uPA effect on oviductal epithelial cells.
abstract: Urokinase type plasminogen activator (uPA) is an oviductal fluid component whose activity is regulated by binding to urokinase type plasminogen activator receptor (uPAR). In this study uPAR and uPA gene expression in bovine oviduct were evaluated and similar expression patterns for both uPAR and uPA mRNAs were observed during the estrous cycle. Immunolocalization of uPAR at the apical zone of epithelial cells suggests that uPA action would be focalized in the oviductal lumen, triggering intracellular signaling pathways. As uPAR expression was also observed in in vitro cultures of oviductal epithelial cells, the effect of uPA was explored using this culture model. Real-time RT-PCR demonstrated that c-fos expression in oviductal cell cultures increases under uPA stimulation. These results suggest that uPA/uPAR binding would be involved in signaling pathways that activate transcription factors and would regulate the synthesis of molecules concerned with the arrangement of a particular oviductal microenvironment.
authors: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT; Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Chacabuco 461, T4000ILI - San Miguel de Tucumán, Argentina.
authors: D C
authors: DC
authors: García
authors: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT; Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Chacabuco 461, T4000ILI - San Miguel de Tucumán, Argentina.
authors: D C
authors: DC
authors: Miceli
authors: Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Chacabuco 461, T4000ILI - San Miguel de Tucumán, Argentina.
authors: P A
authors: PA
authors: Valdecantos
authors: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT; Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Chacabuco 461, T4000ILI - San Miguel de Tucumán, Argentina.
authors: E V
authors: EV
authors: García
authors: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT; Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Chacabuco 461, T4000ILI - San Miguel de Tucumán, Argentina. Electronic address: emroldanolarte@fbqf.unt.edu.ar.
authors: M
authors: M
authors: Roldán-Olarte
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D004847:Epithelial Cells / Q000378:metabolism*; D030762:Estrous Cycle / Q000378:metabolism*; D005187:Fallopian Tubes / Q000166:cytology / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D009841:Oligonucleotides / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D055293:Receptors, Urokinase Plasminogen Activator / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
chemical_list: D009841:Oligonucleotides; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D055293:Receptors, Urokinase Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator
keywords: Bovine; Culture; Oviduct; c-fos; uPA; uPAR