title: Occurrence in Mexico, 1998-2008, of Vibrio cholerae CTX+ El Tor carrying an additional truncated CTX prophage.
abstract: The seventh cholera pandemic caused by Vibrio cholerae O1 El Tor (ET) has been superseded in Asia and Africa by altered ET possessing the cholera toxin (CTX) gene of classical (CL) biotype. The CL biotype of V. cholerae was isolated, along with prototypic and altered ET, during the 1991 cholera epidemic in Mexico and subsequently remained endemic until 1997. Microbiological, molecular, and phylogenetic analyses of clinical and environmental V. cholerae isolated in Mexico between 1998 and 2008 revealed important genetic events favoring predominance of ET over CL and altered ET. V. cholerae altered ET was predominant after 1991 but not after 2000. V. cholerae strains isolated between 2001 and 2003 and a majority isolated in 2004 lacked CTX prophage (Φ) genes encoding CTX subunits A and B and repeat sequence transcriptional regulators of ET and CL biotypes: i.e., CTXΦ(-). Most CTXΦ(-) V. cholerae isolated in Mexico between 2001 and 2003 also lacked toxin coregulated pili tcpA whereas some carried either tcpA(ET) or a variant tcpA with noticeable sequence dissimilarity from tcpA(CL). The tcpA variants were not detected in 2005 after CTXΦ(+) ET became dominant. All clinical and environmental V. cholerae O1 strains isolated during 2005-2008 in Mexico were CTXΦ(+) ET, carrying an additional truncated CTXΦ instead of RS1 satellite phage. Despite V. cholerae CTXΦ(-) ET exhibiting heterogeneity in pulsed-field gel electrophoresis patterns, CTXΦ(+) ET isolated during 2004-2008 displayed homogeneity and clonal relationship with V. cholerae ET N16961 and V. cholerae ET isolated in Peru.
authors: International Center for Diarrheal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka 1212, Bangladesh;
authors: Munirul
authors: M
authors: Alam
authors: International Center for Diarrheal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka 1212, Bangladesh;
authors: Shah Manzur
authors: SM
authors: Rashed
authors: International Center for Diarrheal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka 1212, Bangladesh;
authors: Shahnewaj Bin
authors: SB
authors: Mannan
authors: International Center for Diarrheal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka 1212, Bangladesh;
authors: Tarequl
authors: T
authors: Islam
authors: Centro de Investigación Científica y de Educación Superior de Ensenada, 22860 Ensenada, Baja California, Mexico;
authors: Marcial Leonardo
authors: ML
authors: Lizarraga-Partida
authors: Faculty of Medicine, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico;
authors: Gabriela
authors: G
authors: Delgado
authors: Faculty of Medicine, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico;
authors: Rosario
authors: R
authors: Morales-Espinosa
authors: Faculty of Medicine, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico;
authors: Jose Luis
authors: JL
authors: Mendez
authors: Faculty of Medicine, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico;
authors: Armando
authors: A
authors: Navarro
authors: National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan;
authors: Haruo
authors: H
authors: Watanabe
authors: National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan;
authors: Makoto
authors: M
authors: Ohnishi
authors: Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742;CosmosID, College Park, MD 20742;
authors: Nur A
authors: NA
authors: Hasan
authors: Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742;
authors: Anwar
authors: A
authors: Huq
authors: Johns Hopkins Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21205;
authors: R Bradley
authors: RB
authors: Sack
authors: Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742;CosmosID, College Park, MD 20742;Johns Hopkins Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21205;and Center for Bioinformatics and Computational Biology, University of Maryland Institute for Advanced Computer Studies, University of Maryland, College Park, MD 20742 rcolwell@umiacs.umd.edu.
authors: Rita R
authors: RR
authors: Colwell
authors: International Center for Diarrheal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka 1212, Bangladesh;
authors: Alejandro
authors: A
authors: Cravioto
mesh_terms: D016000:Cluster Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D039002:Prophages / Q000235:genetics*; D014734:Vibrio cholerae / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity