title: Dynamic effects of point source electroporation on the rat brain tissue.
abstract: In spite of aggressive therapy, existing treatments offer poor prognosis for glioblastoma multiforme due to tumor infiltration into the surrounding brain as well as poor blood-brain barrier penetration of most therapeutic agents. In this paper we present a novel approach for a minimally invasive treatment and a non-invasive response assessment methodology consisting of applying intracranial point-source electroporation and assessing treatment effect volumes using magnetic resonance imaging. Using a unique setup of a single intracranial electrode and an external surface electrode we treated rats' brains with various electroporation protocols and applied magnetic resonance imaging to study the dependence of the physiological effects on electroporation treatment parameters. The extent of blood-brain barrier disruption and later volumes of permanent brain tissue damage were found to correlate significantly with the treatment voltages (r(2)=0.99, p<0.001) and the number of treatment pulses (r(2)=0.94, p<0.002). Blood-brain barrier disruption depicted 3.2±0.3 times larger volumes than the final permanent damage volumes (p<0.0001). These results indicate that it may be beneficial to use more than one modality of electroporation when planning a treatment for brain tumors.
authors: The Advanced Technology Center, Sheba Medical Center, Ramat-Gan 52621, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
authors: Shirley
authors: S
authors: Sharabi
authors: The Advanced Technology Center, Sheba Medical Center, Ramat-Gan 52621, Israel.
authors: David
authors: D
authors: Last
authors: The Advanced Technology Center, Sheba Medical Center, Ramat-Gan 52621, Israel.
authors: David
authors: D
authors: Guez
authors: The Advanced Technology Center, Sheba Medical Center, Ramat-Gan 52621, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
authors: Dianne
authors: D
authors: Daniels
authors: Center for Bioengineering in the Service of Humanity and Society, School of Computer Science and Engineering, Hebrew University, Jerusalem, Israel; The Medical Imaging Department, Al Quds University, Abu Dis Jerusalem, Israel.
authors: Mohammad Ibrahim
authors: MI
authors: Hjouj
authors: The Advanced Technology Center, Sheba Medical Center, Ramat-Gan 52621, Israel.
authors: Sharona
authors: S
authors: Salomon
authors: Leviev Heart Center, Sheba Medical Center, Ramat-Gan 52621, Israel; Pinchas Borenstein Talpiot Medical Leadership Program, Sheba Medical Center, Ramat-Gan 52621, Israel.
authors: Elad
authors: E
authors: Maor
authors: The Advanced Technology Center, Sheba Medical Center, Ramat-Gan 52621, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel. Electronic address: yael.mardor@sheba.health.gov.il.
authors: Yael
authors: Y
authors: Mardor
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000473:pathology; D001921:Brain / Q000473:pathology*; D001932:Brain Neoplasms / Q000473:pathology / Q000628:therapy*; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D005909:Glioblastoma / Q000473:pathology / Q000628:therapy*; D008279:Magnetic Resonance Imaging; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
keywords: Blood brain barrier; Brain; Electroporation; MRI; Tumor