title: N-glycosylation modification of plant-derived virus-like particles: an application in vaccines.
abstract: Plants have been developed as an alternative system to mammalian cells for production of recombinant prophylactic or therapeutic proteins for human and animal use. Effective plant expression systems for recombinant proteins have been established with the optimal combination of gene expression regulatory elements and control of posttranslational processing of recombinant glycoproteins. In plant, virus-like particles (VLPs), viral "empty shells" which maintain the same structural characteristics of virions but are genome-free, are considered extremely promising as vaccine platforms and therapeutic delivery systems. Unlike microbial fermentation, plants are capable of carrying out N-glycosylation as a posttranslational modification of glycoproteins. Recent advances in the glycoengineering in plant allow human-like glycomodification and optimization of desired glycan structures for enhancing safety and functionality of recombinant pharmaceutical glycoproteins. In this review, the current plant-derived VLP approaches are focused, and N-glycosylation and its in planta modifications are discussed.
authors: Plant Systems Engineering Research Center, KRIBB, 125 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Hyun-Soon
authors: 0000-0002-9054-0689
authors: HS
authors: Kim
authors: Plant Systems Engineering Research Center, KRIBB, 125 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Jae-Heung
authors: JH
authors: Jeon
authors: Department of Medicine, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Kyung Jin
authors: KJ
authors: Lee
authors: Department of Medicine, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Kisung
authors: K
authors: Ko
mesh_terms: D000818:Animals; D015870:Gene Expression; D006023:Glycoproteins / Q000276:immunology; D006031:Glycosylation; D006801:Humans; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011499:Protein Processing, Post-Translational; D013678:Technology, Pharmaceutical / Q000379:methods*; D058425:Vaccines, Virus-Like Particle / Q000276:immunology*
chemical_list: D006023:Glycoproteins; D058425:Vaccines, Virus-Like Particle