title: Probiotic properties of Pediococcus strains isolated from jeotgals, salted and fermented Korean sea-food.
abstract: Three Pediococcus pentosaceus strains were isolated from jeotgals, salted and fermented Korean sea-foods, and their probiotic potentials were examined. After 2 h exposure to pH 3.0, P. pentosaceus F66 survived with the survival ratio of 32.6% followed by P. pentosaceus D56 (17.2%) and P. pentosaceus A24 (7.5%). P. pentosaceus F66 also survived better (26.6%) than P. pentosaceus A24 (13.7%) and P. pentosaceus D56 (5.8%) after 2 h exposure to 0.3% bile salts. Three strains grew slowly on MRS broth with 15% NaCl (w/v), reaching the OD600 values of 0.4-0.8 in 36 h. They adhered to Caco-2 cells (10.9-13.9 CFU/cell) with similar degree of adherence of a positive control, Lactobacillus rhamnosus GG (12.8 ± 0.5 CFU/cell). Three strains possess some desirable enzyme activities such as β-galactosidase, α-glucosidase, β-glucosidase, and N-acetyl-β-glucosidase. From these results, P. pentosaceus F66 seems qualified as a probiotic and can be utilized for fermented foods including jeotgals.
authors: Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Kang Wook
authors: KW
authors: Lee
authors: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Ji Yeong
authors: JY
authors: Park
authors: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Hyun Deok
authors: HD
authors: Sa
authors: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Ji Hee
authors: JH
authors: Jeong
authors: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Dong Eun
authors: DE
authors: Jin
authors: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Ho Jin
authors: HJ
authors: Heo
authors: Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea; Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: jeonghkm@gnu.ac.kr.
authors: Jeong Hwan
authors: JH
authors: Kim
mesh_terms: D001422:Bacterial Adhesion; D001647:Bile Acids and Salts / Q000378:metabolism; D018938:Caco-2 Cells; D004798:Enzymes / Q000032:analysis; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007723:Korea; D052201:Lacticaseibacillus rhamnosus; D050296:Microbial Viability / Q000187:drug effects; D010376:Pediococcus / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D019936:Probiotics / Q000494:pharmacology*; D017747:Seafood / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D001647:Bile Acids and Salts; D004798:Enzymes; D012965:Sodium Chloride
keywords: Aggregation ability; Jeotgal; Pediococcus pentosaceus; Probiotic; Stress tolerance