title: Inhibition of lung tumor growth in nude mice by siRNACD31targeting PECAM-1.
abstract: Small interfering RNA (siRNA) provides a promising therapeutic approach in the silencing of disease-causing genes. In the present study, the use of 2'-O-methyl-modified siRNA-cluster of differentiation 31 (siRNACD31), with cationic liposome RNA interference (RNAi)-mate as a carrier, effectively silenced the platelet endothelial cell molecule 1 (PECAM-1) gene of murine hemangioendothelioma cellsin vitro. In vivo, 2'-O-methyl-modified siRNACD31carried by RNAi-mate was successfully delivered, targeting the PECAM-1 gene in the vasculature of nude mouse lung carcinoma xenografts. The growth of the lung carcinoma xenografts was inhibited by the 2'-O-methyl-modified siRNACD31and RNAi-mate complexes, and the expression of the PECAM-1 protein was downregulated, with a simultaneous decrease in vascular endothelial growth factor (VEGF) protein in the lung carcinoma xenografts. 2'-O-methyl-modified siRNACD31-RNAi-mate complexes may provide a potential therapeutic strategy in lung carcinoma treatment. The effect of PECAM-1 on VEGF expression may possibly be attributed to the function of PECAM-1 signal transduction.
authors: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 32500, P.R. China.
authors: Jin-Sheng
authors: JS
authors: Ouyang
authors: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 32500, P.R. China.
authors: Yu-Ping
authors: YP
authors: Li
authors: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 32500, P.R. China.
authors: Cheng-Shui
authors: CS
authors: Chen
authors: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 32500, P.R. China.
authors: Jun-Jie
authors: JJ
authors: Chen
authors: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 32500, P.R. China.
authors: Tong-Ke
authors: TK
authors: Chen
authors: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 32500, P.R. China.
authors: Chang
authors: C
authors: Cai
authors: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 32500, P.R. China.
authors: Li
authors: L
authors: Yang
keywords: carcinoma; platelet endothelial adhesion molecule; small interfering RNA; tumor microenvironment; vascular endothelial growth factor