title: Chitosan coated copper and cadmium hexacyanocobaltate nanocubes as immunosensing probes for the construction of multiple analytes platform.
abstract: In this work, Cd3[Co(CN)6]2 and Cu3[Co(CN)6]2 (CdNCs and CuNCs) nanocubes were synthesized simply by a one-step process at room temperature in the presence of chitosan (CS). It was found that CdNCs and CuNCs produced obviously distinctive anodic peak currents at -0.7 V and -0.1 V (vs. Ag/AgCl), whose separation enabled differentiation between two analytes. They were used as novel electrochemical probes in multiplex electrochemical detection for carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) in a single run. The good performance of the new electrochemical probes was obtained. The linearity range was from 0.025 to 250 ng mL(-1) for both CEA and AFP. The detection limit of CEA was 0.0175 ng mL(-1) and that of AFP was 0.0109 ng mL(-1) at a signal-to-noise of 3. Analysis of clinical serum samples using this immunosensor was well consistent with the data determined by the enzyme-linked immunosorbent assay (ELISA). The novel electrochemical probes could be generally used in multiplex protein detections.
authors: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors: Zifeng
authors: Z
authors: Wang
authors: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors: Xia
authors: X
authors: Chen
authors: Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: mazhanfang@cnu.edu.cn.
authors: Zhanfang
authors: Z
authors: Ma
mesh_terms: D002104:Cadmium / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000097:blood*; D048271:Chitosan / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D002272:Carcinoembryonic Antigen; D000509:alpha-Fetoproteins; D002104:Cadmium; D003035:Cobalt; D003300:Copper; D048271:Chitosan
keywords: Alpha-fetoprotein; Carcinoembryonic antigen; Cd(3)[Co(CN)(6)](2); Cu(3)[Co(CN)(6)](2); Electrochemical immunosensor; nanocubes