title: Mutual regulation of TGF-β1, TβRII and ErbB receptors expression in human thyroid carcinomas.
abstract: The role of EGF and TGF-β1 in thyroid cancer is still not clearly defined. TGF-β1 inhibited the cellular growth and migration of follicular (FTC-133) and papillary (B-CPAP) thyroid carcinoma cell lines. Co-treatments of TGF-β1 and EGF inhibited proliferation in both cell lines, but displayed opposite effect on their migratory capability, leading to inhibition in B-CPAP and promotion in FTC-133 cells, by a MAPK-dependent mechanism. TGF-β1, TβRII and EGFR expressions were evaluated in benign and malignant thyroid tumors. Both positivity (51.7% and 60.0% and 80.0% in FA and PTC and FTC) and overexpression (60.0%, 77.7% and 75.0% in FA, PTC and FTC) of EGFR mRNA correlates with the aggressive tumor behavior. The moderate overexpression of TGF-β1 and TβRII mRNA in PTC tissues (61.5% and 62.5%, respectively), counteracted their high overexpression in FTC tissues (100% and 100%, respectively), while EGFR overexpression was similar in both carcinomas. Papillary carcinomas were positive to E-cadherin expression, while the follicular carcinomas lose E-cadherin staining. Our findings of TGF-β1/TβRII and EGFR overexpressions together with a loss of E-cadherin observed in human follicular thyroid carcinomas, and of increased migration ability MAPK-dependent after EGF/TGF-β1 treatments in the follicular thyroid carcinoma cell line, reinforced the hypothesis of a cross-talk between EGF and TGF-β1 systems in follicular thyroid carcinomas phenotype.
authors: Department of Experimental and Clinical Sciences, University 'G. d'Annunzio of Chieti-Pescara, Chieti, Italy; Center of Excellence on Aging, Ce.S.I., 'G. d׳Annunzio' University Foundation, Chieti, Italy. Electronic address: g.mincione@unich.it.
authors: Gabriella
authors: G
authors: Mincione
authors: Department of Experimental and Clinical Sciences, University 'G. d'Annunzio of Chieti-Pescara, Chieti, Italy.
authors: Chiara
authors: C
authors: Tarantelli
authors: Department of Experimental and Clinical Sciences, University 'G. d'Annunzio of Chieti-Pescara, Chieti, Italy; Center of Excellence on Aging, Ce.S.I., 'G. d׳Annunzio' University Foundation, Chieti, Italy.
authors: Giovina
authors: G
authors: Vianale
authors: Department of Experimental and Clinical Sciences, University 'G. d'Annunzio of Chieti-Pescara, Chieti, Italy.
authors: Maria Carmela
authors: MC
authors: Di Marcantonio
authors: Department of Experimental and Clinical Sciences, University 'G. d'Annunzio of Chieti-Pescara, Chieti, Italy; Unit of General and Laparoscopic Surgery, SS Annunziata Hospital, Chieti, Italy.
authors: Roberto
authors: R
authors: Cotellese
authors: Unit of General and Laparoscopic Surgery, SS Annunziata Hospital, Chieti, Italy.
authors: Franco
authors: F
authors: Francomano
authors: Department of Experimental and Clinical Sciences, University 'G. d'Annunzio of Chieti-Pescara, Chieti, Italy.
authors: Marta
authors: M
authors: Di Nicola
authors: Department of Experimental and Clinical Sciences, University 'G. d'Annunzio of Chieti-Pescara, Chieti, Italy.
authors: Erica
authors: E
authors: Costantini
authors: Unit of General and Laparoscopic Surgery, SS Annunziata Hospital, Chieti, Italy.
authors: Annadomenica
authors: A
authors: Cichella
authors: Department of Experimental and Clinical Sciences, University 'G. d'Annunzio of Chieti-Pescara, Chieti, Italy; Center of Excellence on Aging, Ce.S.I., 'G. d׳Annunzio' University Foundation, Chieti, Italy.
authors: Raffaella
authors: R
authors: Muraro
mesh_terms: D000328:Adult; D000368:Aged; D015820:Cadherins / Q000235:genetics; D002277:Carcinoma / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D004815:Epidermal Growth Factor / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*; D013964:Thyroid Neoplasms / Q000235:genetics*; D053773:Transforming Growth Factor beta1 / Q000235:genetics*; D055815:Young Adult
chemical_list: D015820:Cadherins; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D004815:Epidermal Growth Factor; C512478:EGFR protein, human; D066246:ErbB Receptors; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II
keywords: EGF; ErbB receptors; Human thyroid carcinoma; TGF-β1; TβRII