title: Cell-imprinted substrates act as an artificial niche for skin regeneration.
abstract: Bioinspired materials can mimic the stem cell environment and modulate stem cell differentiation and proliferation. In this study, biomimetic micro/nanoenvironments were fabricated by cell-imprinted substrates based on mature human keratinocyte morphological templates. The data obtained from atomic force microscopy and field emission scanning electron microscopy revealed that the keratinocyte-cell-imprinted poly(dimethylsiloxane) casting procedure could imitate the surface morphology of the plasma membrane, ranging from the nanoscale to the macroscale, which may provide the required topographical cell fingerprints to induce differentiation. Gene expression levels of the genes analyzed (involucrin, collagen type I, and keratin 10) together with protein expression data showed that human adipose-derived stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape and characteristics of keratinocytes. The observed morphology of the ADSCs grown on the keratinocyte casts was noticeably different from that of stem cells cultivated on the stem-cell-imprinted substrates. Since the shape and geometry of the nucleus could potentially alter the gene expression, we used molecular dynamics to probe the effect of the confining geometry on the chain arrangement of simulated chromatin fibers in the nuclei. The results obtained suggested that induction of mature cell shapes onto stem cells can influence nucleus deformation of the stem cells followed by regulation of target genes. This might pave the way for a reliable, efficient, and cheap approach of controlling stem cell differentiation toward skin cells for wound healing applications.
authors: Department of Medical Nanotechnology, School of Advanced Technologies in Medicine (SATiM), Tehran University of Medical Sciences , P.O. Box 14177-55469, Tehran, Iran.
authors: Omid
authors: O
authors: Mashinchian
authors: Shahin
authors: S
authors: Bonakdar
authors: Hossein
authors: H
authors: Taghinejad
authors: Vahid
authors: V
authors: Satarifard
authors: Maziar
authors: M
authors: Heidari
authors: Mohammad
authors: M
authors: Majidi
authors: Shahriar
authors: S
authors: Sharifi
authors: Afshin
authors: A
authors: Peirovi
authors: Samaneh
authors: S
authors: Saffar
authors: Mohammad
authors: M
authors: Taghinejad
authors: Mohammad
authors: M
authors: Abdolahad
authors: Shams
authors: S
authors: Mohajerzadeh
authors: Mohammad Ali
authors: MA
authors: Shokrgozar
authors: Seyed Mahdi
authors: SM
authors: Rezayat
authors: Mohammad R
authors: MR
authors: Ejtehadi
authors: Matthew J
authors: MJ
authors: Dalby
authors: Morteza
authors: M
authors: Mahmoudi
mesh_terms: D016475:3T3 Cells; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002469:Cell Separation; D048430:Cell Shape / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000494:pharmacology; D020869:Gene Expression Profiling; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008648:Mesoderm / Q000187:drug effects; D051379:Mice; D018625:Microscopy, Atomic Force; D056004:Molecular Dynamics Simulation; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration; D012828:Silicones / Q000494:pharmacology; D012867:Skin / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon