title: Biological role of Trichoderma harzianum-derived platelet-activating factor acetylhydrolase (PAF-AH) on stress response and antagonism.
abstract: We investigated the properties of platelet-activating factor acetylhydrolase (PAF-AH) derived from Trichoderma harzianum. The enzyme, comprised of 572 amino acids, shares high homology with PAF-AH proteins from T. koningii and other microbial species. The optimum enzymatic activity of PAF-AH occurred at pH 6 in the absence of Ca2+ and it localized in the cytoplasm, and we observed the upregulation of PAF-AH expression in response to carbon starvation and strong heat shock. Furthermore, PAF-AH knockout transformant growth occurred more slowly than wild type cells and over-expression strains grown in SM medium at 37°C and 42°C. In addition, PAF-AH expression significantly increased under a series of maize root induction assay. Eicosanoic acid and ergosterol levels decreased in the PAF-AH knockouts compared to wild type cells, as revealed by GC/MS analysis. We also determined stress responses mediated by PAF-AH were related to proteins HEX1, Cu/Zn superoxide dismutase, and cytochrome c. Finally, PAF-AH exhibited antagonistic activity against Rhizoctonia solani in plate confrontation assays. Our results indicate PAF-AH may play an important role in T. harzianum stress response and antagonism under diverse environmental conditions.
authors: Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
authors: Chuanjin
authors: C
authors: Yu
authors: Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
authors: Lili
authors: L
authors: Fan
authors: Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
authors: Qiong
authors: Q
authors: Wu
authors: Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
authors: Kehe
authors: K
authors: Fu
authors: Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
authors: Shigang
authors: S
authors: Gao
authors: Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
authors: Meng
authors: M
authors: Wang
authors: Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
authors: Jinxin
authors: J
authors: Gao
authors: Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
authors: Yaqian
authors: Y
authors: Li
authors: Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
authors: Jie
authors: J
authors: Chen
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D005227:Fatty Acids / Q000378:metabolism; D055786:Gene Knockout Techniques; D042541:Intracellular Space / Q000378:metabolism; D021381:Protein Transport; D012232:Rhizoctonia / Q000502:physiology; D013261:Sterols / Q000378:metabolism; D013312:Stress, Physiological; D014170:Transformation, Genetic; D014242:Trichoderma / Q000166:cytology / Q000201:enzymology* / Q000502:physiology*
chemical_list: D005227:Fatty Acids; D013261:Sterols; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase