title: Oxidized extracellular DNA suppresses nitric oxide production by endothelial NO synthase (eNOS) in human endothelial cells (HUVEC).
abstract: Circulating DNA from patients with cardiovascular diseases reduce the synthesis of NO in endothelial cells, which is probably related to oxidative modification of DNA. To test this hypothesis, HUVEC cells were cultured in the presence of DNA containing ~1 (nonoxidized DNA), 700, or 2100 8-oxodG/10(6) nucleosides. Nonoxidized DNA stimulated the synthesis of NO, which was associated with an increase in the expression of endothelial NO synthase. Oxidized NO decreased the amount of mRNA and protein for endothelial NO synthase, but increased the relative content of its low active form. These changes were accompanied by reduction of NO production. These findings suggest that oxidative modification of circulating extracellular DNA contributes to endothelial dysfunction manifested in suppression of NO production.
authors: Medical Genetic Research Center, Russian Academy of Medical Sciences, Moscow, Russia.
authors: S V
authors: SV
authors: Kostyuk
authors: A Yu
authors: AY
authors: Alekseeva
authors: M S
authors: MS
authors: Kon'kova
authors: K V
authors: KV
authors: Glebova
authors: T D
authors: TD
authors: Smirnova
authors: L V
authors: LV
authors: Kameneva
authors: V L
authors: VL
authors: Izhevskaya
authors: N N
authors: NN
authors: Veiko
mesh_terms: D002478:Cells, Cultured; D042783:Endothelial Cells / Q000201:enzymology*; D061307:Human Umbilical Vein Endothelial Cells / Q000201:enzymology*; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III