title: Genome-scale analysis of the high-efficient protein secretion system of Aspergillus oryzae.
abstract: BACKGROUND
  The koji mold, Aspergillus oryzae is widely used for the production of industrial enzymes due to its particularly high protein secretion capacity and ability to perform post-translational modifications. However, systemic analysis of its secretion system is lacking, generally due to the poorly annotated proteome.
  
  
  RESULTS
  Here we defined a functional protein secretory component list of A. oryzae using a previously reported secretory model of S. cerevisiae as scaffold. Additional secretory components were obtained by blast search with the functional components reported in other closely related fungal species such as Aspergillus nidulans and Aspergillus niger. To evaluate the defined component list, we performed transcriptome analysis on three α-amylase over-producing strains with varying levels of secretion capacities. Specifically, secretory components involved in the ER-associated processes (including components involved in the regulation of transport between ER and Golgi) were significantly up-regulated, with many of them never been identified for A. oryzae before. Furthermore, we defined a complete list of the putative A. oryzae secretome and monitored how it was affected by overproducing amylase.
  
  
  CONCLUSIONS
  In combination with the transcriptome data, the most complete secretory component list and the putative secretome, we improved the systemic understanding of the secretory machinery of A. oryzae in response to high levels of protein secretion. The roles of many newly predicted secretory components were experimentally validated and the enriched component list provides a better platform for driving more mechanistic studies of the protein secretory pathway in this industrially important fungus.
authors: Lifang
authors: L
authors: Liu
authors: Amir
authors: A
authors: Feizi
authors: Tobias
authors: T
authors: Österlund
authors: Carsten
authors: C
authors: Hjort
authors: Novo Nordisk Foundation Center for Biosustainability, Department of Chemical and Biologicl Engineering, Chalmers University of Technology, SE-41296 Göteborg, Sweden. nielsenj@chalmers.se.
authors: Jens
authors: J
authors: Nielsen
mesh_terms: D001236:Aspergillus oryzae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D005656:Fungal Proteins / Q000378:metabolism*; D016681:Genome, Fungal / Q000235:genetics; D023281:Genomics; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000096:biosynthesis
chemical_list: D005656:Fungal Proteins; D000516:alpha-Amylases