title: Does moxifloxacin alter oxidant status in the cornea? An experimental study.
abstract: OBJECTIVE
  In this experimental study, we investigated the possible effects of intracameral moxifloxacin on oxidative stress parameters and endothelial cell morphology in corneal tissue.
  
  
  METHODS
  In total, 30 rats were randomly assigned to three groups of 10 rats: the sham group (Group 1, n = 10); the control group (Group 2), where balanced salt solution (BSS) was administered at a dose of 0.01 cc (n = 10); and the treatment group (Group 3), where moxifloxacin was administered at a dose of 0.05 mg/0.01 cc (n = 10). Total antioxidant status (TAS) and total oxidant status (TOS) in corneal tissue and blood samples were measured and the oxidative stress index (OSI) was calculated. Also, corneal tissue histopathology was evaluated with caspase-3 and caspase-8 staining. Apoptotic activity was also evaluated.
  
  
  RESULTS
  In blood samples, TAS, TOS, and OSI levels were not statistically significantly different (all p > 0.05). Compared with the sham and control groups, TOS and OSI levels in cornea tissue were significantly different in the moxifloxacin group (all p < 0.05). However, compared with the control group, no statistically significant difference was found in the sham group (all p > 0.05). Compared with the sham and control groups, apoptotic activity was higher in the moxifloxacin group, in both immunohistochemical staining for caspase-3 and caspase-8.
  
  
  CONCLUSIONS
  Intracameral moxifloxacin injection seems to be safe systemically, but it may have toxic effects on corneal tissues, as suggested by oxidative stress parameters and a histopathological evaluation.
authors: Department of Ophthalmology .
authors: Ali
authors: A
authors: Akal
authors: Turgay
authors: T
authors: Ulas
authors: Tugba
authors: T
authors: Goncu
authors: Muhammet Emin
authors: ME
authors: Guldur
authors: Sezen
authors: S
authors: Kocarslan
authors: Abdullah
authors: A
authors: Taskin
authors: Emin
authors: E
authors: Savik
authors: Ufuk
authors: U
authors: Ozkan
authors: Emel Yigit
authors: EY
authors: Karakas
authors: Mete
authors: M
authors: Koksal
authors: Nurten
authors: N
authors: Aksoy
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism; D003315:Cornea / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D024841:Fluoroquinolones / Q000494:pharmacology*; D008297:Male; D000077266:Moxifloxacin; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D016877:Oxidants; D020169:Caspases; D000077266:Moxifloxacin
keywords: Apoptosis; cornea; intracameral moxifloxacin; oxidative stress