title: Production of the anti-inflammatory compound 6-O-palmitoyl-3-O-β-D-glucopyranosylcampesterol by Callus cultures of Lopezia racemosa Cav. (Onagraceae).
abstract: Lopezia racemosa Cav. is a plant used in Mexican traditional medicine to heal inflammatory diseases. From this plant we isolated the novel compound 6-O-palmitoyl- 3-O-β-D-glucopyranosylcampesterol (1) and 6-O-palmitoyl-3-O-β-D-glucopyranosyl-β-sitosterol (2), previously reported to have cytotoxic activity on several cancer cell lines. We evaluated the anti-inflammatory activity of 1 in vivo by mouse ear edema induced with 12-O-tetradecanoylphorbol-13-acetate (TPA) and 57.14% inhibition was observed. The aim of our study was to obtain callus cultures derived from this plant species with the ability to produce the compounds of interest. Callus cultures were initiated on MS basal medium amended with variable amounts of naphthaleneacetic acid (NAA), or 2,4-dichlorophenoxyacetic acid (2,4-D), combined or not with 6-benzylaminopurine (BAP). Ten treatments with these growth regulators were carried out, using in vitro germinated seedlings as source of three different explants: hypocotyl, stem node, and leaf. Highest yield of 1 was observed on callus derived from leaf explants growing in medium containing 1.0 mg/L 2,4-D and 0.5 mg/L BAP. Selected callus lines produced less 1 than wild plants but the in vitro cultured seedlings showed higher production. So we conclude that it could be attractive to further investigate their metabolic potential.
authors: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca CP 62209, Morelos, Mexico. robertsm76@gmail.com.
authors: Roberta
authors: R
authors: Salinas
authors: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca CP 62209, Morelos, Mexico. jesus.arellano@uaem.mx.
authors: Jesús
authors: J
authors: Arellano-García
authors: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca CP 62209, Morelos, Mexico. iperea@uaem.mx.
authors: Irene
authors: I
authors: Perea-Arango
authors: Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca CP 62209, Morelos, Mexico. lalvarez@uaem.mx.
authors: Laura
authors: L
authors: Alvarez
authors: Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca CP 62209, Morelos, Mexico. lgarduno@uaem.mx.
authors: María Luisa
authors: ML
authors: Garduño-Ramírez
authors: Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca CP 62209, Morelos, Mexico. smarquina21@hotmail.com.
authors: Silvia
authors: S
authors: Marquina
authors: Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Xochitepec CP 62790, Morelos, Mexico. azamilpa_2000@yahoo.com.mx.
authors: Alejandro
authors: A
authors: Zamilpa
authors: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca CP 62209, Morelos, Mexico. castillo@uaem.mx.
authors: Patricia
authors: P
authors: Castillo-España
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D002478:Cells, Cultured; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004423:Ear / Q000473:pathology; D004487:Edema / Q000188:drug therapy; D018525:Germination / Q000502:physiology; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D051379:Mice; D029594:Onagraceae / Q000378:metabolism*; D064209:Phytochemicals / Q000494:pharmacology; D010840:Phytosterols / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate
chemical_list: D000893:Anti-Inflammatory Agents; D064209:Phytochemicals; D010840:Phytosterols; D010936:Plant Extracts; C021273:campesterol; D002784:Cholesterol; D013755:Tetradecanoylphorbol Acetate