title: Genome sequence of Ensifer meliloti strain WSM1022; a highly effective microsymbiont of the model legume Medicago truncatula A17.
abstract: Ensifer meliloti WSM1022 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Medicago. WSM1022 was isolated in 1987 from a nodule recovered from the roots of the annual Medicago orbicularis growing on the Cyclades Island of Naxos in Greece. WSM1022 is highly effective at fixing nitrogen with M. truncatula and other annual species such as M. tornata and M. littoralis and is also highly effective with the perennial M. sativa (alfalfa or lucerne). In common with other characterized E. meliloti strains, WSM1022 will nodulate but fixes poorly with M. polymorpha and M. sphaerocarpos and does not nodulate M. murex. Here we describe the features of E. meliloti WSM1022, together with genome sequence information and its annotation. The 6,649,661 bp high-quality-draft genome is arranged into 121 scaffolds of 125 contigs containing 6,323 protein-coding genes and 75 RNA-only encoding genes, and is one of 100 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
authors: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors: Jason
authors: J
authors: Terpolilli
authors: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors: Yvette
authors: Y
authors: Hill
authors: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors: Rui
authors: R
authors: Tian
authors: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors: John
authors: J
authors: Howieson
authors: School of Life and Environmental Sciences, Deakin University, Victoria, Australia.
authors: Lambert
authors: L
authors: Bräu
authors: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors: Lynne
authors: L
authors: Goodwin
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: James
authors: J
authors: Han
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Konstantinos
authors: K
authors: Liolios
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Marcel
authors: M
authors: Huntemann
authors: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors: Amrita
authors: A
authors: Pati
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Tanja
authors: T
authors: Woyke
authors: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors: Konstantinos
authors: K
authors: Mavromatis
authors: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors: Victor
authors: V
authors: Markowitz
authors: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors: Natalia
authors: N
authors: Ivanova
authors: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors: Nikos
authors: N
authors: Kyrpides
authors: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors: Wayne
authors: W
authors: Reeve
keywords: Alphaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria