title: Increasing effect of Tangzhiqing formula on IRS-1-dependent PI3K/AKT signaling in muscle.
abstract: BACKGROUND
  Tangzhiqing fomula (TZQ-F), the mixture of Red Paeony root, Mulberry leaf, Lotus leaf, Danshen root and Hawthorn leaf, regulates the abnormal glucose and lipids in prediabetic patients. In this study, we focus on the mechanism of TZQ-F and its fractions on glucose metabolism.
  
  
  METHODS
  After orally administration of TZQ-F for 4 weeks in KK-Ay mice, we dissected out the liver and muscle, and employed PCR and western blotting to screening the PI3K/AKT pathway. The following PI3K/AKT signaling pathway were performed in L-6 myotube and HepG2 cells.
  
  
  RESULTS
  In the liver of KK-Ay mice, no significance was observed on PI3K, AKT and their phosphorylation between TZQ-F and controls , while, in the muscle, up-regulation of PI3K, AKT, Glycogen synthase (GYS) and their phosphorylation type, as well as GluT4, was deteced in TZQ-F. In HepG2 cells, TZQ-F increased IRS-2 by 10 folds, without interrupting AKT, IRS-1 and GluT4. In L-6 myotube cells, TZQ-F and its fractions treatment significantly increased IRS-1 and AKT at mRNA level.
  
  
  CONCLUSIONS
  TZQ-F prevents pre-diabetes through increasing effect on IRS-1-dependent PI3K/AKT signaling pathway in muscle.
authors: Jing
authors: J
authors: Gao
authors: Jian
authors: J
authors: Li
authors: Yating
authors: Y
authors: An
authors: Xuefeng
authors: X
authors: Liu
authors: Qian
authors: Q
authors: Qian
authors: Yanlin
authors: Y
authors: Wu
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China. zhwwxzh@263.net.
authors: Yi
authors: Y
authors: Zhang
authors: Tao
authors: T
authors: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D006006:Glycogen Synthase / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D055504:Insulin Receptor Substrate Proteins; D014280:Triglycerides; C583958:tangzhiqing; D006006:Glycogen Synthase; D019869:Phosphatidylinositol 3-Kinases