title: Formylchromone exhibits salubrious effects against nitrosodiethylamine mediated early hepatocellular carcinogenesis in rats.
abstract: The salubrious effects of 3-Formylchromone (3-FC) against nitrosodiethylamine (NDEA) mediated early hepatocellular carcinogenesis was investigated in vivo by this study. Male Wistar rats were administered three interspersed intraperitoneal injections of NDEA (200 mg/kg body weight) until sixth week, followed by, thrice a week oral dose of 3-FC (25 mg/kg body weight) from the seventh week to eleventh week. Oral supplementation of Wistar rats with 3-FC prevented the increase in serum marker enzymes (AST, ALT, LDH) and serum pre-neoplastic marker (γ-GT) induced by NDEA. Biochemical observations were found to be further correlated with histological studies, indicating the potential of 3-FC to mediate suppression of hepatic damage/pre-neoplastic lesions. Argyrophilic nucleolar organizer region (AgNOR) staining was done in histology sections to confirm the anti-proliferative potential of 3-FC against NDEA-induced early hepatocellular carcinogenesis. RT-PCR and immunoblot analysis was done to find the modulations in the gene transcript/protein level expression of pre-neoplastic marker (GST-pi), proliferation marker (PCNA), apoptotic mediators (PPARγ, NFκB-p65 and p53). 3-FC was found to favorably modulate the expressions of GST-pi, PCNA, PPARγ, NFκB-p65, p53 clearly confirming the anti-proliferative and apoptotic potential of 3-FC. Further, the apoptotic effect of 3-FC against NDEA-induced early hepatocellular carcinogenesis was confirmed by caspase-3 activity assay and DNA fragmentation analysis. Based on these findings, it is concluded that 3-FC possesses hepatoprotective, anti-pre-neoplastic, anti-proliferative and apoptosis inducing capability against NDEA-induced early hepatocellular carcinogenesis.
authors: Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur 613402, Tamil Nadu, India.
authors: M
authors: M
authors: Ilamathi
authors: Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur 613402, Tamil Nadu, India.
authors: S
authors: S
authors: Senthilkumar
authors: Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur 613402, Tamil Nadu, India; Central Animal Facility, SASTRA University, Thanjavur 613402, Tamil Nadu, India.
authors: P C
authors: PC
authors: Prabu
authors: Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur 613402, Tamil Nadu, India; Central Animal Facility, SASTRA University, Thanjavur 613402, Tamil Nadu, India.
authors: S
authors: S
authors: Panchapakesan
authors: Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur 613402, Tamil Nadu, India. Electronic address: sivaramakrishnan@scbt.sastra.edu.
authors: V
authors: V
authors: Sivaramakrishnan
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001219:Aspartate Aminotransferases / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced* / Q000188:drug therapy / Q000473:pathology; D002867:Chromones / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004052:Diethylnitrosamine / Q000378:metabolism / Q000633:toxicity; D051549:Glutathione S-Transferase pi / Q000235:genetics / Q000378:metabolism; D006651:Histocytochemistry; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000188:drug therapy / Q000473:pathology; D008297:Male; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000235:genetics; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D002867:Chromones; D047495:PPAR gamma; D018809:Proliferating Cell Nuclear Antigen; D051996:Transcription Factor RelA; C502480:formylchromone; D004052:Diethylnitrosamine; D012313:RNA; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D051549:Glutathione S-Transferase pi; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: 3-Formylchromone; AgNOR; Anti-proliferative; Apoptosis; Hepatoprotective; Pre-neoplastic lesions