title: LIM and SH3 domain protein 1 (LASP-1) overexpression was associated with aggressive phenotype and poor prognosis in clear cell renal cell cancer.
abstract: BACKGROUND
  LIM and SH3 protein 1 (LASP-1) is a specific focal adhesion protein that is known to be involved in numerous biological and pathological processes. LASP-1 overexpression has been described in several types of cancers, but its expression and role in clear cell renal cell cancer (ccRCC) remains unknown.
  
  
  METHODS
  Using immunohistochemistry, we analyzed LASP-1 protein expression in 216 clinicopathologically characterized ccRCC cases. We also examined LASP-1 expression in 20 paired ccRCC tissues and in 2 cell lines by real-time PCR and Western blot. Using RNA interference, we investigated the effects of LASP-1 depletion on tumor cell behavior in vitro. Statistical analyses were used to determine the associations between LASP-1 levels, tumor features and patient outcomes.
  
  
  RESULTS
  LASP-1 overexpression was observed in ccRCC tissues (P<0.0001) compared to adjuvant nontumorous tissues, and its expression levels were closely correlated with overall survival and recurrence-free survival (P = 0.044 and 0.006, respectively) in patients with ccRCC. RNA interference-mediated silencing of the LASP-1 gene in 786-0 ccRCC cells significantly inhibited cell migration.
  
  
  CONCLUSIONS
  The results of the present study indicate that LASP-1 may serve as a prognostic biomarker for ccRCC patients and may be a promising target for the treatment of ccRCC.
authors: Department of Urology Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors: Fan
authors: F
authors: Yang
authors: State Key Laboratory of Cancer Biology and Experimental Teaching Center of Basic Medicine, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors: Xingchun
authors: X
authors: Zhou
authors: Department of Urology Surgery, Peoples' Hospital of Shaanxi Province, Xi'an, Shaanxi, China.
authors: Shuangkuan
authors: S
authors: Du
authors: Department of Urology Surgery, Peoples' Hospital of Shaanxi Province, Xi'an, Shaanxi, China.
authors: Yongjun
authors: Y
authors: Zhao
authors: Department of Urology Surgery, Peoples' Hospital of Shaanxi Province, Xi'an, Shaanxi, China.
authors: Wei
authors: W
authors: Ren
authors: Department of Urology Surgery, Peoples' Hospital of Shaanxi Province, Xi'an, Shaanxi, China.
authors: Qian
authors: Q
authors: Deng
authors: Department of Urology Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors: Fuli
authors: F
authors: Wang
authors: Department of Urology Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors: Jianlin
authors: J
authors: Yuan
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D007680:Kidney Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009364:Neoplasm Recurrence, Local / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D003598:Cytoskeletal Proteins; C096353:LASP1 protein, human; D060588:LIM Domain Proteins; D012333:RNA, Messenger