title: Methionine deficiency reduces autophagy and accelerates death in intestinal epithelial cells infected with enterotoxigenic Escherichia coli.
abstract: Infections by enterotoxigenic Escherichia coli (ETEC) result in large economic losses to the swine industry worldwide. Dietary supplementation with amino acids has been considered as a potential mechanism to improve host defenses against infection. The goal of this study was to determine whether methionine deprivation alters ETEC interactions with porcine intestinal epithelial cells. IPEC-1 cells were cultured in media with or without L-methionine. Methionine deprivation resulted in enhanced ETEC adhesion and increased both the cytotoxicity and apoptotic responses of IPEC-1 cells infected with ETEC. Methionine deprivation inhibited IPEC-1 cell autophagic responses, suggesting that the increased cytotoxicity of ETEC to methionine-deprived IPEC-1 cells might be due to defects in autophagy.
authors: Key Lab Agro-ecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Changsha, Hunan, Peoples Republic of China.
authors: Yulong
authors: Y
authors: Tang
authors: Key Lab Agro-ecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Changsha, Hunan, Peoples Republic of China.
authors: Bie
authors: B
authors: Tan
authors: Key Lab Agro-ecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Changsha, Hunan, Peoples Republic of China. xx@isa.ac.cn.
authors: Xia
authors: X
authors: Xiong
authors: Key Lab Agro-ecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Changsha, Hunan, Peoples Republic of China.
authors: Fengna
authors: F
authors: Li
authors: Key Lab Agro-ecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Changsha, Hunan, Peoples Republic of China.
authors: Wenkai
authors: W
authors: Ren
authors: Key Lab Agro-ecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Changsha, Hunan, Peoples Republic of China.
authors: Xiangfeng
authors: X
authors: Kong
authors: Hunan New Wellful Co., Ltd, Changsha, 410001, Hunan, China.
authors: Wei
authors: W
authors: Qiu
authors: Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, 66506, USA.
authors: Philip R
authors: PR
authors: Hardwidge
authors: Key Lab Agro-ecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Changsha, Hunan, Peoples Republic of China. yinyulong@isa.ac.cn.
authors: Yulong
authors: Y
authors: Yin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy; D001422:Bacterial Adhesion / Q000187:drug effects; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D054307:Enterotoxigenic Escherichia coli / Q000472:pathogenicity*; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology / Q000473:pathology*; D004927:Escherichia coli Infections / Q000382:microbiology*; D007422:Intestines / Q000187:drug effects / Q000382:microbiology / Q000473:pathology*; D008715:Methionine / Q000172:deficiency* / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013552:Swine
chemical_list: D008715:Methionine
keywords: Apoptosis; Autophagy; Cytotoxicity; Enterotoxigenic Escherichia coli; IPEC-1; Methionine