title: Culture supernatants of different colon cancer cell lines induce specific phenotype switching and functional alteration of THP-1 cells.
abstract: We developed an in vitro model to evaluate the effect of products secreted from different colorectal cancer (CRC) cell lines on specific phenotypic switching and functional alterations in THP-1 cells. We co-cultured the human monocytic cell line, THP-1, or phorbol-12-myristate-13-acetate (PMA)-treated THP-1 cells, (THP-1p), with supernatants from either the HT-29 (Dukes' B), HCT-15 (Dukes' C), or Colo205 (Dukes' D) cell lines, and assessed the cells for macrophage differentiation. The surface marker and cytokine profiles suggested that secreted CRC factors differentiated THP-1 cells into a "mixed" M1/M2 phenotype, although HT-29 and Colo205 supernatants induced THP-1p cells into predominantly M1-like macrophages and M2-like macrophages, respectively. Further, all three CRC supernatants enhanced the phagocytic capacity and migration of THP-1 and THP-1p cells, altering their phenotype to a more M2-kind. Therefore, different CRC cell lines induced specific phenotype switching and functional polarization of THP-1 cells.
authors: Division of Hemato-oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Keelung & Chang Gung University, College of Medicine, Keelung, Taiwan.
authors: Tsung-Han
authors: TH
authors: Wu
authors: Division of Hemato-oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Keelung & Chang Gung University, College of Medicine, Keelung, Taiwan.
authors: Ying-Ying
authors: YY
authors: Li
authors: Division of Hemato-oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Keelung & Chang Gung University, College of Medicine, Keelung, Taiwan.
authors: Tai-Ling
authors: TL
authors: Wu
authors: Division of Hemato-oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Linkou & Chang Gung University, College of Medicine, Taiwan.
authors: John W-C
authors: JW
authors: Chang
authors: Division of Hemato-oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Linkou & Chang Gung University, College of Medicine, Taiwan.
authors: Wen-Chi
authors: WC
authors: Chou
authors: Institute of Basic Medicine, Medical College of Chang Gung University and Chang Gung Memorial Hospital, Linkou, Taiwan.
authors: Ling-Ling
authors: LL
authors: Hsieh
authors: Department of Pathology, Chang Gung Memorial Hospital, Keelung & Chang Gung University, College of Medicine, Keelung, Taiwan.
authors: Jim-Ray
authors: JR
authors: Chen
authors: Division of Hemato-oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Keelung & Chang Gung University, College of Medicine, Keelung, Taiwan. Electronic address: yehtyng@gmail.com.
authors: Kun-Yun
authors: KY
authors: Yeh
mesh_terms: D000954:Antigens, Surface / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis; D019073:HT29 Cells; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D010587:Phagocytosis / Q000276:immunology; D010641:Phenotype; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives
chemical_list: D000954:Antigens, Surface; D017077:Culture Media, Conditioned; D016207:Cytokines; C016658:phorbolol myristate acetate; D013755:Tetradecanoylphorbol Acetate
keywords: Colorectal cancer; Differentiation; Migration; Phagocytosis; Tumor-associated macrophages