title: [Proliferation and osteogenic differentiation of mesenchymal stem cells in hydrogels of human blood plasma].
abstract: BACKGROUND
  The use of mesenchymal stem cells in clinical practice has increased considerably in the last decade because they play a supporting role in the processes of tissue repair and regeneration, becoming the main tool of cell therapy for the treatment of diseases functionally affecting bone and cartilage tissue .
  
  
  OBJECTIVE
  To evaluate in vitro the proliferative and osteogenic differentiation ability of mesenchymal stem cells derived from human adipose tissue in a blood plasma hydrogel.
  
  
  METHODS
  Mesenchymal stem cells were obtained from human adipose tissue explants and characterized by flow cytometry. Their multipotentiality was demonstrated by their ability to differentiate to adipogenic and osteogenic lineages. Cell proliferation and osteogenic differentiation ability of the cells cultured in blood plasma hydrogels were also evaluated.
  
  
  RESULTS
  Mesenchymal stem cells derived from human adipose tissue growing in human blood plasma hydrogels showed a pattern of proliferation similar to that of the cells cultured in monolayer and also maintained their ability to differentiate to osteogenic lineage.
  
  
  CONCLUSIONS
  Human blood plasma hydrogels are a suitable support for proliferation and osteogenic differentiation of mesenchymal stem cells derived from human adipose tissue and provides a substrate that is autologous, biocompatible, reabsorbable, easy to use, potentially injectable and economic, which could be used as a successful strategy for the management and clinical application of cell therapy in regenerative medicine.
authors: Grupo de Investigación Biología de Células Madre, Universidad Nacional de Colombia, Bogotá, D.C, Colombia.
authors: Itali M
authors: IM
authors: Linero
authors: Grupo de Investigación Biología de Células Madre, Universidad Nacional de Colombia, Bogotá, D.C, Colombia.
authors: Adriana
authors: A
authors: Doncel
authors: Grupo de Investigación Biología de Células Madre, Universidad Nacional de Colombia, Bogotá, D.C, Colombia.
authors: Orlando
authors: O
authors: Chaparro
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D001769:Blood; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D020100:Hydrogels; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010012:Osteogenesis
chemical_list: D003470:Culture Media; D020100:Hydrogels