title: Temporospatial expression of fibulin-1 after acute spinal cord injury in rats.
abstract: OBJECTIVE
  Fibulin-1 is a matricellular protein that plays important roles in motility inhibition in a variety of cells and blocks the proliferation of cultured neural stem cells. The biological function of fibulin-1 in the spinal cord has not been fully elucidated.
  
  
  METHODS
  To clarify the expressions and possible functions of fibulin-1 in spinal cord injury (SCI), we performed an acute spinal cord contusion injury model in adult rats. Our work studied the temporospatial expression patterns of fibulin-1.
  
  
  RESULTS
  Western blot analysis revealed that fibulin-1 levels significantly increased 5 days after spinal cord contusion. Immunohistochemistry confirmed an increased number of fibulin-1 immunopositive cells about 2 mm from the lesion site. Moreover, double immunofluorescence labeling suggested that these changes were especially prominent in neurons and microglia.
  
  
  CONCLUSIONS
  These findings suggest that fibulin-1 may be involved in neuronal apoptosis and microglial activation after SCI.
authors: Guanhua
authors: G
authors: Xu
authors: Ying
authors: Y
authors: Cui
authors: Lingling
authors: L
authors: Wang
authors: Jinlong
authors: J
authors: Zhang
authors: Aiguo
authors: A
authors: Shen
authors: Weidong
authors: W
authors: Li
authors: Guofeng
authors: G
authors: Bao
authors: Yuyu
authors: Y
authors: Sun
authors: Zhiming
authors: Z
authors: Cui
mesh_terms: D000818:Animals; D017209:Apoptosis; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; C060820:fibulin
keywords: Fibulin-1; Glial proliferation; Neuron apoptosis; Rat; Spinal cord injury