title: Silver nanoparticles impair Peste des petits ruminants virus replication.
abstract: In the present study, we evaluated the antiviral efficacy of the silver nanoparticles (SNPs) against Peste des petits ruminants virus (PPRV), a prototype Morbillivirus. The leaf extract of the Argemone maxicana was used as a reducing agent for biological synthesis of the SNPs from silver nitrate. The SNPs were characterized using UV-vis absorption spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM). The TEM analysis revealed particle size of 5-30 nm and the XRD analysis revealed their characteristic silver structure. The treatment of Vero cells with the SNPs at a noncytotoxic concentration significantly inhibited PPRV replication in vitro. The time-course and virus step-specific assays showed that the SNPs impair PPRV replication at the level of virus entry. The TEM analysis showed that the SNPs interact with the virion surface as well with the virion core. However, this interaction has no direct virucidal effect, instead exerts a blocking effect on viral entry into the target cells. This is the first documented evidence indicating that the SNPs are capable of inhibiting a Morbillivirus replication in vitro.
authors: School of Biotechnology, Rajiv Gandhi Technical University, Airport Road, Bhopal, Madhya Pradesh 462036, India.
authors: Nitin
authors: N
authors: Khandelwal
authors: School of Biotechnology, Rajiv Gandhi Technical University, Airport Road, Bhopal, Madhya Pradesh 462036, India.
authors: Gurpreet
authors: G
authors: Kaur
authors: Virology Laboratory, Division of Animal Health, Central Institute for Research on Goats, Indian Council of Agricultural Research, Makhdoom, Mathura, Uttar Pradesh 281122, India.
authors: Kundan Kumar
authors: KK
authors: Chaubey
authors: School of Biotechnology, Rajiv Gandhi Technical University, Airport Road, Bhopal, Madhya Pradesh 462036, India.
authors: Pushpendra
authors: P
authors: Singh
authors: Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125004, India.
authors: Shalini
authors: S
authors: Sharma
authors: School of Biotechnology, Rajiv Gandhi Technical University, Airport Road, Bhopal, Madhya Pradesh 462036, India.
authors: Archana
authors: A
authors: Tiwari
authors: Virology Laboratory, Division of Animal Health, Central Institute for Research on Goats, Indian Council of Agricultural Research, Makhdoom, Mathura, Uttar Pradesh 281122, India.
authors: Shoor Vir
authors: SV
authors: Singh
authors: Virology Laboratory, Division of Animal Health, Central Institute for Research on Goats, Indian Council of Agricultural Research, Makhdoom, Mathura, Uttar Pradesh 281122, India. Electronic address: naveenkumar.icar@gmail.com.
authors: Naveen
authors: N
authors: Kumar
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D053768:Metal Nanoparticles / Q000737:chemistry*; D029021:Peste-des-Petits-Ruminants / Q000821:virology*; D018107:Peste-des-petits-ruminants virus / Q000187:drug effects* / Q000502:physiology; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D012834:Silver
keywords: Antiviral activity; PPR virus; Peste des petits ruminants; SNPs; Silver nanoparticles