title: Comparison of expression of secondary metabolite biosynthesis cluster genes in Aspergillus flavus, A. parasiticus, and A. oryzae.
abstract: Fifty six secondary metabolite biosynthesis gene clusters are predicted to be in the Aspergillus flavus genome. In spite of this, the biosyntheses of only seven metabolites, including the aflatoxins, kojic acid, cyclopiazonic acid and aflatrem, have been assigned to a particular gene cluster. We used RNA-seq to compare expression of secondary metabolite genes in gene clusters for the closely related fungi A. parasiticus, A. oryzae, and A. flavus S and L sclerotial morphotypes. The data help to refine the identification of probable functional gene clusters within these species. Our results suggest that A. flavus, a prevalent contaminant of maize, cottonseed, peanuts and tree nuts, is capable of producing metabolites which, besides aflatoxin, could be an underappreciated contributor to its toxicity.
authors: Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, New Orleans, LA 70124, USA. ehrlich8@gmail.com.
authors: Kenneth C
authors: KC
authors: Ehrlich
authors: Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, New Orleans, LA 70124, USA. brian.mack@ars.usda.gov.
authors: Brian M
authors: BM
authors: Mack
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis; D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D001230:Aspergillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001231:Aspergillus flavus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001236:Aspergillus oryzae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D018556:Crops, Agricultural / Q000382:microbiology; D005516:Food Microbiology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D009183:Mycotoxins / Q000096:biosynthesis*; D011042:Poisons / Q000378:metabolism*; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism; D012331:RNA, Fungal / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D064210:Secondary Metabolism; D013045:Species Specificity; D035165:Transcription Factors, General / Q000235:genetics / Q000378:metabolism
chemical_list: D000348:Aflatoxins; D005656:Fungal Proteins; D009183:Mycotoxins; D011042:Poisons; D012331:RNA, Fungal; D012333:RNA, Messenger; D035165:Transcription Factors, General; D048630:Polyketide Synthases; D019883:Alkyl and Aryl Transferases; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; C032409:tryptophan dimethylallyltransferase