title: Evaluation of the separation performance of polyvinylpyrrolidone as a virtual stationary phase for chromatographic NMR.
abstract: Polyvinylpyrrolidone (PVP) was used as a virtual stationary phase to separate p-xylene, benzyl alcohol, and p-methylphenol by the chromatographic NMR technique. The effects of concentration and weight-average molecular weight (Mw) of PVP, solvent viscosity, solvent polarity, and sample temperature on the resolution of these components were investigated. It was found that both higher PVP concentration and higher PVP Mw caused the increase of diffusion resolution for the three components. Moreover, the diffusion resolution did not change at viscosity-higher solvents. Moreover, the three components showed different resolution at different solvents. As temperature increased, the diffusion resolution between p-xylene and benzyl alcohol gradually increased, and the one between p-xylene and p-methylphenol slightly increased from 278 to 298 K and then decreased above 298 K. It was also found that the polarity of the analytes played an important role for the separation by affecting the diffusion coefficient.
authors: Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
authors: Shaohua
authors: S
authors: Huang
authors: Rui
authors: R
authors: Wu
authors: Zhengwu
authors: Z
authors: Bai
authors: Ying
authors: Y
authors: Yang
authors: Suying
authors: S
authors: Li
authors: Xiaowei
authors: X
authors: Dou
mesh_terms: D002251:Carbon Tetrachloride; D002845:Chromatography / Q000379:methods*; D004058:Diffusion; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008970:Molecular Weight; D011205:Povidone / Q000737:chemistry*; D013696:Temperature; D014783:Viscosity
chemical_list: D007202:Indicators and Reagents; D002251:Carbon Tetrachloride; D011205:Povidone
keywords: 1H; NMR; chromatographic NMR; diffusion resolution; polyvinylpyrrolidone; virtual stationary phase