title: Microbiota from Litopenaeus vannamei: digestive tract microbial community of Pacific white shrimp (Litopenaeus vannamei).
abstract: Bacteria capable of producing different extracellular enzymes of potential relevance in digestive processes were isolated from the stomach, hepatopancreas and intestine of Pacific white shrimp Litopenaeus vannamei. A total of 64 strains with proteolytic activity were isolated and grouped into 16 clusters based on morphological characteristics: 4 groups were isolated from the intestine; 5 from the hepatopancreas; and 7 from the stomach. Molecular methods (16S rRNA gene amplification and sequencing) and phenotypic criteria (Gram stain, catalase and oxidase tests, cell and colony morphology) were used to identify strains, which corresponded to Pseudoalteromonas and Vibrio genera. These genera are reported to form part of the digestive tract microbial community in shrimp. Both genera were isolated from all three tested tissues. One member of each morphologic group was selected for analysis of the presence of amylases, lipases/esterases and chitinases. Most of the strains had all the tested enzymes, indicating that the L. vannamei digestive tract microbiotic flora includes groups which have the potential to contribute to the degradation of dietary components.
authors: Unidad Multidisciplinaria de Docencia e Investigación (UMDI), Universidad Nacional Autónoma de México (UNAM), Puerto de Abrigo s/n, Sisal, Yucatan Mexico.
authors: Jaqueline Tuyub
authors: JT
authors: Tzuc
authors: Campus de Ingenierías y Ciencias Exactas, Universidad Autónoma de Yucatán. Facultad de Ingeniería Química, Periférico Norte Kilómetro 33.5, Tablaje Catastral 13615, Col. Chuburná de Hidalgo Inn., C.P. 97203 Mérida, Yucatán México.
authors: Diana Rendíz
authors: DR
authors: Escalante
authors: Campus de Ingenierías y Ciencias Exactas, Universidad Autónoma de Yucatán. Facultad de Ingeniería Química, Periférico Norte Kilómetro 33.5, Tablaje Catastral 13615, Col. Chuburná de Hidalgo Inn., C.P. 97203 Mérida, Yucatán México.
authors: Rafael
authors: R
authors: Rojas Herrera
authors: Unidad Multidisciplinaria de Docencia e Investigación (UMDI), Universidad Nacional Autónoma de México (UNAM), Puerto de Abrigo s/n, Sisal, Yucatan Mexico.
authors: Gabriela
authors: G
authors: Gaxiola Cortés
authors: Unidad Multidisciplinaria de Docencia e Investigación (UMDI), Universidad Nacional Autónoma de México (UNAM), Puerto de Abrigo s/n, Sisal, Yucatan Mexico.
authors: Maria Leticia Arena
authors: ML
authors: Ortiz
keywords: 16S; Digestion; Enzymatic activity; Microbiota; Shrimp