title: Establishment and characterization of new orthotopic and metastatic neuroblastoma models.
abstract: OBJECTIVE
  Treatment of metastatic neuroblastoma remains a challenge in pediatric oncology. Relevant preclinical models may improve exploration of oncogenesis and new therapies. We developed new orthotopic and metastatic models derived from stage 4 neuroblastoma.
  
  
  METHODS
  Orthotopic and systemic models were established in BalbC Rag2(-/-)gammaC(-/-) mice following adrenal and intravenous injection of luciferase-transfected IMR-32 and IGR-N91 cells, respectively.
  
  
  RESULTS
  All four models exhibited 100% tumor take rate. Metastatic spread of orthotopic IMR-32-Luc cells was observed mainly to the lung, liver and bone; that of IGR-N91-Luc cells to liver, spleen and adrenals. Interestingly, systemic IMR-32-Luc cells metastasized rather to the lung, liver and bone, and IGR-N91-Luc to liver, lung, spleen and adrenals. Feasibility of non-invasive, real-time antitumor response evaluation was validated in the systemic models.
  
  
  CONCLUSIONS
  These neuroblastoma models with distinct patterns of metastatic spread represent relevant tools for exploring local and metastatic tumor cell tropism, mechanisms of spread and evaluating new cancer therapeutics.
authors: Vectorology and Anticancer Therapeutics, UMR 8203, University Paris-Sud, Orsay, France CNRS, Orsay, Vectorology and Anticancer Therapeutics, UMR 8203, Orsay, France Gustave Roussy Institute, Vectorology and Anticancer Therapeutics, UMR 8203, Villejuif, France.
authors: Estelle
authors: E
authors: Daudigeos-Dubus
authors: Vectorology and Anticancer Therapeutics, UMR 8203, University Paris-Sud, Orsay, France CNRS, Orsay, Vectorology and Anticancer Therapeutics, UMR 8203, Orsay, France Gustave Roussy Institute, Vectorology and Anticancer Therapeutics, UMR 8203, Villejuif, France.
authors: Ludivine
authors: L
authors: LE Dret
authors: Imaging and Cytometry Platform, UMR 8081 IR4M, Gustave Roussy Institute, Villejuif, France.
authors: Valérie
authors: V
authors: Rouffiac
authors: Plateform of Preclinical Evaluation, Gustave Roussy Institute, Villejuif, France.
authors: Olivia
authors: O
authors: Bawa
authors: IR4M, UMR 8081, Paris-Sud University, CNRS, Orsay, France.
authors: Ingrid
authors: I
authors: Leguerney
authors: Plateform of Preclinical Evaluation, Gustave Roussy Institute, Villejuif, France.
authors: Paule
authors: P
authors: Opolon
authors: Vectorology and Anticancer Therapeutics, UMR 8203, University Paris-Sud, Orsay, France CNRS, Orsay, Vectorology and Anticancer Therapeutics, UMR 8203, Orsay, France Gustave Roussy Institute, Vectorology and Anticancer Therapeutics, UMR 8203, Villejuif, France.
authors: Gilles
authors: G
authors: Vassal
authors: Vectorology and Anticancer Therapeutics, UMR 8203, University Paris-Sud, Orsay, France CNRS, Orsay, Vectorology and Anticancer Therapeutics, UMR 8203, Orsay, France Gustave Roussy Institute, Vectorology and Anticancer Therapeutics, UMR 8203, Villejuif, France Department of Pediatric and Adolescent Oncology, Gustave Roussy Institute, Villejuif, France birgit.geoerger@gustaveroussy.fr.
authors: Birgit
authors: B
authors: Geoerger
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology; D001706:Biopsy; D002166:Camptothecin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D015870:Gene Expression; D017930:Genes, Reporter; D064593:Heterografts; D006801:Humans; D000077146:Irinotecan; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements / Q000379:methods; D051379:Mice; D018345:Mice, Knockout; D009362:Neoplasm Metastasis; D009447:Neuroblastoma / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000077146:Irinotecan; D008156:Luciferases; D002166:Camptothecin
keywords: Orthotopic and systemic human xenograft model; bioluminescence imaging; metastasis pattern; neuroblastoma