title: Yi qi qing re gao attenuates podocyte injury and inhibits vascular endothelial growth factor overexpression in puromycin aminonucleoside rat model.
abstract: Proteinuria is the hallmark of chronic kidney disease. Podocyte damage underlies the formation of proteinuria, and vascular endothelial growth factor (VEGF) functions as an autocrine/paracrine regulator. Yi Qi Qing Re Gao (YQQRG) has been used to treat proteinuria for more than two decades. The objective of this study was to investigate the protective effect and possible mechanisms of YQQRG on puromycin aminonucleoside (PAN) rat model. Eighty male Sprague-Dawley rats were randomized into sham group, PAN group, PAN + YQQRG group, and PAN + fosinopril group. Treatments were started 7 days before induction of nephrosis (a single intravenous injection of 40 mg/kg PAN) until day 15. 24 h urinary samples were collected on days 5, 9, and 14. The animals were sacrificed on days 3, 10, and 15, respectively. Blood samples and renal tissues were obtained for detection of biochemical and molecular biological parameters. YQQRG significantly reduced proteinuria, elevated serum albumin, and alleviated renal pathological lesions. YQQRG inhibited VEGF-A, nephrin, podocin, and CD2AP mRNA expression and elevated nephrin, podocin, and CD2AP protein levels starting on day 3. In conclusion, YQQRG attenuates podocyte injury in the rat PAN model through downregulation of VEGF-A and restoration of nephrin, podocin, and CD2AP protein expression.
authors: Department of Nephrology, Guang'anmen Hospital of China Academy of Traditional Chinese Medical Sciences, No. 5 Beixiange, Xicheng District, Beijing 100053, China.
authors: Yongli
authors: Y
authors: Zhan
authors: Department of Nephrology, Guang'anmen Hospital of China Academy of Traditional Chinese Medical Sciences, No. 5 Beixiange, Xicheng District, Beijing 100053, China.
authors: Liping
authors: L
authors: Yang
authors: Beijing University of Chinese Medicine, No. 11 Beisanhuan Donglu, Chaoyang District, Beijing 100029, China.
authors: Yumin
authors: Y
authors: Wen
authors: Beijing University of Chinese Medicine, No. 11 Beisanhuan Donglu, Chaoyang District, Beijing 100029, China.
authors: Huijie
authors: H
authors: Liu
authors: Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, No. 2 Yinghua Dongjie, Hepingli, Beijing 100029, China.
authors: Haojun
authors: H
authors: Zhang
authors: Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, No. 2 Yinghua Dongjie, Hepingli, Beijing 100029, China.
authors: Bin
authors: B
authors: Zhu
authors: Beijing University of Chinese Medicine, No. 11 Beisanhuan Donglu, Chaoyang District, Beijing 100029, China.
authors: Wenbing
authors: W
authors: Han
authors: Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, No. 2 Yinghua Dongjie, Hepingli, Beijing 100029, China.
authors: Yanting
authors: Y
authors: Gu
authors: Beijing University of Chinese Medicine, No. 11 Beisanhuan Donglu, Chaoyang District, Beijing 100029, China.
authors: Xueyan
authors: X
authors: Sun
authors: Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, No. 2 Yinghua Dongjie, Hepingli, Beijing 100029, China.
authors: Xi
authors: X
authors: Dong
authors: Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, No. 2 Yinghua Dongjie, Hepingli, Beijing 100029, China.
authors: Tingting
authors: T
authors: Zhao
authors: Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, No. 2 Yinghua Dongjie, Hepingli, Beijing 100029, China.
authors: Huixia
authors: H
authors: Ma
authors: Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, No. 2 Yinghua Dongjie, Hepingli, Beijing 100029, China.
authors: Ping
authors: P
authors: Li