title: Evaluation of soluble CD200 levels in type 2 diabetic foot and nephropathic patients: association with disease activity.
abstract: BACKGROUND
  CD200 (OX-2) is a novel immune-effective molecule, existing in a cell membrane-bound form, as well as in a soluble form in serum (s OX-2), which acts to regulate inflammatory and acquired immune responses.
  
  
  METHODS
  We planned this study to evaluate the sOX-2 levels of type 2 diabetic foot (group B), and compare it with that of healthy controls (group A). The patient group had the following values: DM period: 27.9±10.3 year [mean ±SD], HbA1c: 9.52±2.44% [mean ±SD].
  
  
  RESULTS
  Blood samples for sCD200 measurement were always taken in the morning between 8 and 10 A.M.. The results were reported as means of duplicate measurements. Concentrations of sOX-2 in the serum samples were quantified using an ELISA kit. Serum hs-CRP levels were measured using an hs-CRP assay kit. The sOX-2 level in group B was 173.8±3.1 and in group A was 70.52±1.2 [p<0.0001). In subgroup analysis of T2DM-DFI patients, we noticed that sOX-2 levels were higher in WGS (Wagner grading system) I and II patients than in WGS III and IV patients. The HbA1c, BUN, creatinine, hs-CRP levels, and sedimentation rates were higher in the patient group (p<0.0001, p<0.001, p<0.001, p<0.005, and p<0.0001, respectively).
  
  
  CONCLUSIONS
  We suggest that there are vascular, immunologic, and neurologic components in DFI, whereas autoimmune diseases and inflammatory skin disorders have only an immunologic component. This is possibly evidence of a pro-inflammatory effect seen in DFI as a vascular complication.
authors: Orthopaedics and Traumatology Clinic, Yozgat State Hospital, Yozgat, Turkey.
authors: Hasan Onur
authors: HO
authors: Arik
authors: Department of Internal Medicine, Allergy and Clinical Immunology Unit, Genomics Research Center, Academia Sinica, Taipei, Taiwan.
authors: Arzu Didem
authors: AD
authors: Yalcin
authors: Department of Laboratory Medicine and Pathology, Mayo Clinic in Jacksonville, Jacksonville, USA.
authors: Betul
authors: B
authors: Celik
authors: Department of Infectious Disease, Antalya Training and Reseach Hospital, Antalya, Turkey.
authors: Derya
authors: D
authors: Seyman
authors: Department of Plastic Surgery, Antalya Education and Research Hospital, Antalya, Turkey.
authors: Gulsum
authors: G
authors: Tetik
authors: Infection Disease Clinic, Tekirdag State Hospital, Tekirdag, Turkey.
authors: Bensu
authors: B
authors: Gursoy
authors: Department of Infection, Allergy and Clinical Immunology Unit, Tepecik Education and Research Hospital, Izmir, Turkey.
authors: Sukran
authors: S
authors: Kose
authors: Department of Medical Biochemistry, Faculty of Medicine, Akdeniz University., Antalya, Turkey.
authors: Saadet
authors: S
authors: Gumuslu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000097:blood*; D016022:Case-Control Studies; D017719:Diabetic Foot / Q000097:blood* / Q000473:pathology*; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000097:blood* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012995:Solubility
chemical_list: D015703:Antigens, CD; C042739:antigens, CD200