title: (99m)Tc-labeled porphyrin-lipid nanovesicles.
abstract: Porphyrin-lipid nanovesicles (PLN) have been developed with intrinsic capabilities as activatable multimodal photonic contrast agents. Radiolabeling of PLN encapsulating drugs could eventually be able to provide quantitative in vivo information for diagnosing and treating diseases. In this study, we developed (99m)Tc-labeled porphyrin-lipid nanovesicles ((99m)Tc-PLN) as a cargo-encapsulated formulation without significant impact on liposome integrity and encapsulation stability. 50 mM calcein was encapsulated into PLN by probe sonication. The size of the PLN was about 150 nm. The PLN were then reacted with (99m)Tc using SnCl2 dissolved in 1 mM HCl as a reducing agent and incubated for 10 min at 22 °C. The radiolabeling efficiency and stability of (99m)Tc-PLN were evaluated by instant thin-layer chromatography and low-pressure liquid chromatography (LPLC). (99m)Tc labeling was successful with a >92% labeling efficiency. LPLC showed that the liposomal elution peaks of the porphyrin-lipid and the calcein overlapped with the radioactivity elution peak of (99m)Tc-labeled PLN. The (99m)Tc-labeling procedure did not change the size of PLN. Encapsulated calcein remained inert inside PLN. Thus, this work lays out a simple and effective radiolabeling method using SnCl2 in HCl in the preparation of (99m)Tc-PLN.
authors: Radiopharmaceutical Laboratory, Division of Nuclear Medicine, Department of Radiology and Imaging Sciences, Warren G. Magnuson Clinical Center, National Institutes of Health , Bethesda, MD , USA and.
authors: Jae-Ho
authors: JH
authors: Lee
authors: Shuai
authors: S
authors: Shao
authors: Kenneth T
authors: KT
authors: Cheng
authors: Jonathan F
authors: JF
authors: Lovell
authors: Chang H
authors: CH
authors: Paik
mesh_terms: D008055:Lipids / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D013667:Technetium / Q000737:chemistry*
chemical_list: D008055:Lipids; D011166:Porphyrins; D013667:Technetium
keywords: 99mTc-labeled porphyrin–lipid nanovesicles; Drug-encapsulated liposome; nanotechnology; size exclusion analysis; sustained release