title: Negative predictive value of the chorionic villous sampling (CVS) in diagnosis of thalassemia in genetic laboratory of Dastgheib Hospital, Shiraz, Iran, 2012.
abstract: BACKGROUND
  Chorionic Villous Sampling (CVS) is a diagnostic method for determining genetic disorders. The present study aimed to determine the negative predictive value of the CVS in the diagnosis of major thalassemia in genetic laboratory of Dastgheib Hospital, Shiraz, Iran.
  
  
  METHODS
  The present research was an evaluation diagnostic test conducted on 372 records of embryos examined through CVS in the genetic lab in 2010 and definitely diagnosed by electrophoresis after birth in 2012. The sensitivity and positive predictive value of the test were assessed for minor thalassemia. The negative predictive value and the specificity of this test were determined, as well.
  
  
  RESULTS
  A total of 3 embryos (0.8%) were aborted due to testing. In this study, the sensitivity and specificity were 94.8% and 80.4%, respectively. Also, the negative predictive values for diagnosis of major and minor thalassemia were 100% and 89.2%, respectively. No relationships were found between the gestational age and the test results.
  
  
  CONCLUSIONS
  The results of this study showed that CVS genetic testing in genetic laboratory of Dastgheib Hospital was valid and had a high diagnostic value. Thus, minor couples can undergo this test with relative safety in order to prevent major thalassemia.
authors: School of Health, Shiraz University of Medical Sciences, Shiraz, Iran. mm59m@yahoo.com.
authors: Fariba
authors: F
authors: Ghahramani
authors: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: Yousef
authors: Y
authors: Alimohamadi
authors: Kermanshah University of Medical Sciences, Kermanshah, Iran, Abadan College of Medical Sciences and Health Services, Abadan, Iran.
authors: Mohammad
authors: M
authors: Mahboubi
authors: Shahid Dastgheib Hospital Genetic Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: Abdolreza
authors: A
authors: Afrasiabi
mesh_terms: D000755:Anemia, Sickle Cell / Q000175:diagnosis* / Q000235:genetics; D015193:Chorionic Villi Sampling; D005260:Female; D006801:Humans; D007492:Iran; D008297:Male; D011237:Predictive Value of Tests; D011247:Pregnancy; D012680:Sensitivity and Specificity; D013789:Thalassemia / Q000175:diagnosis / Q000235:genetics; D017086:beta-Thalassemia / Q000175:diagnosis* / Q000235:genetics