title: Conjugated-polymer-based red-emitting nanoparticles for two-photon excitation cell imaging with high contrast.
abstract: Two-photon fluorescence microscopy is a widely used noninvasive bioimaging technique because of unique advantages such as a large penetration depth and 3D mapping capability. Ideal two-photon fluorophores require large two-photon absorption cross sections and red emission with high quantum yields. Here we report red-emitting-dye-doped conjugated polymer nanoparticles that display high two-photon excitation brightness. In these nanoparticles, conjugated polymer (PFV) was chosen as a two-photon light-harvesting material, and red-emitting dyes (MgPc and Nile red) were chosen as the energy acceptors and red-emitting materials. Two-photon excitation fluorescence of MgPc and Nile red was enhanced by up to ∼53 and ∼240 times, respectively. We have successfully demonstrated the application of these conjugated polymer-based nanoparticles in two-photon excitation cancer cell imaging with an excellent contrast ratio. This concept could become a general approach to the preparation of two-photon excitation red-emitting materials for deep-tissue live-cell imaging with high contrast.
authors: Department of Chemistry, National University of Singapore , 3 Science Drive 3, Republic of Singapore 117543.
authors: Shuang
authors: S
authors: Li
authors: Xiaoqin
authors: X
authors: Shen
authors: Lin
authors: L
authors: Li
authors: Peiyan
authors: P
authors: Yuan
authors: Zhenping
authors: Z
authors: Guan
authors: Shao Q
authors: SQ
authors: Yao
authors: Qing-Hua
authors: QH
authors: Xu
mesh_terms: D003287:Contrast Media / Q000737:chemistry*; D003952:Diagnostic Imaging / Q000379:methods*; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D011108:Polymers