title: [Determination of single nucleotide mutations in the 23S rRNA gene of Helicobacter pylori related to clarithromycin resistance in a population from Cauca, Colombia].
abstract: BACKGROUND
  Antibiotic combination therapy for the eradication of Helicobacter pylori should be based on local resistance patterns.
  
  
  OBJECTIVE
  To d etermine the resistance of H. pylori to clarithromycin in a population from Cauca province, through the identification of mutations in the 23S rRNA gene in DNA from gastric biopsies.
  
  
  METHODS
  A total of 162 patients with functional dyspepsia were included in the study. The 23S rRNA gene and the DNA from 162 gastric specimens were amplified by PCR, and the mutation pattern was identified by direct sequencing.
  
  
  RESULTS
  The frequency of clarithromycin resistance was 4%. A2143G mutation was found in four patients (2.46%) and A2142G mutation was found in three patients (1.85%).
  
  
  CONCLUSIONS
  Our study shows that the most frequent genotype in H. pylori -positive specimens was A2143G, followed by A2142G. The observed resistance prevalence of H. pylori was low; thus, we consider that clarithromycin treatment is a valid option for H. pylori eradication in the study population.
authors: Grupo de Investigación en Genética Humana Aplicada, Facultad de Ciencias de la Salud, Universidad del Cauca, Popayán, Colombia.
authors: Claudia Patricia
authors: CP
authors: Acosta
authors: Fundación InnovaGen, Popayán, Colombia.
authors: Fabián Andrés
authors: FA
authors: Hurtado
authors: Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C, Colombia.
authors: Alba Alicia
authors: AA
authors: Trespalacios
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001706:Biopsy; D017291:Clarithromycin / Q000494:pharmacology*; D003105:Colombia / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005260:Female; D005756:Gastritis / Q000453:epidemiology / Q000382:microbiology* / Q000473:pathology; D005798:Genes, Bacterial; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D012329:RNA, Bacterial / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; C074689:VacA protein, Helicobacter pylori; D017291:Clarithromycin