title: Characterization of lake minnow Eupallasella percnurus semen in relation to sperm morphology, regulation of sperm motility and interpopulation diversity.
abstract: Sperm morphology and regulation of sperm motility of lake minnow Eupallasella percnurus, an endangered cyprinid, were investigated. Milt characteristics from two isolated populations of E. percnurus were compared to characterize the interpopulation diversity. Electron microscopic studies revealed that E. percnurus spermatozoa comprise simple, uniflagellate, anacrosomal aquasperm with species-specific features as an eccentrically located implantation of nuclear fossa and eccentric insertion of flagellum. Sperm motility was significantly inhibited by relatively low ion concentrations (150, 150 and 8 mM for NaCl, KCl and CaCl2 , respectively). Sperm maintained a high motility rate over a wide pH range (5.5-10.5), which may reflect adaptation to a highly variable environment. The two E. percnurus populations were markedly different in milt volume, sperm concentration, seminal plasma pH, sperm motility and beat cross frequency, which may result from genetic differences and environmental conditions.
authors: Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.
authors: G J
authors: GJ
authors: Dietrich
authors: A
authors: A
authors: Pecio
authors: J
authors: J
authors: Sikorska
authors: J
authors: J
authors: Wolnicki
authors: M
authors: M
authors: Słowińska
authors: P
authors: P
authors: Hliwa
authors: R
authors: R
authors: Kamiński
authors: A
authors: A
authors: Andronowska
authors: A
authors: A
authors: Ciereszko
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D060106:Lakes; D008297:Male; D008854:Microscopy, Electron; D012661:Semen / Q000737:chemistry*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure
keywords: Cyprinidae; isolated populations; sperm morphometry; sperm quality