title: MoxT toxin of Bacillus anthracis exhibits sequence specific ribonuclease activity.
abstract: MoxXT module of Bacillus anthracis encodes MoxX, a labile protein and MoxT, a ribonuclease. However, mechanism of cleavage of RNA by MoxT has not been explored till date. In the present study, we have demonstrated that MoxT is a sequence specific ribonuclease which recognizes UACAU sequence in ss RNA and cleaves between U and A. Moreover, cleavage of RNA requires 2' OH group of first residue, i.e. U of UACAU RNA sequence. An interesting finding which makes it distinct from the other MazF family toxins was also observed, i.e. its ability to cleave RNA in DNA-RNA hybrid.
authors: Laboratory of Genetic Engineering and Molecular Biology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India. Electronic address: shashikalaverma1@gmail.com.
authors: Shashikala
authors: S
authors: Verma
authors: Laboratory of Genetic Engineering and Molecular Biology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India. Electronic address: rakeshbhatnagar@jnu.ac.in.
authors: Rakesh
authors: R
authors: Bhatnagar
mesh_terms: D001408:Bacillus anthracis / Q000378:metabolism*; D001427:Bacterial Toxins / Q000737:chemistry*; D001483:Base Sequence; D059367:RNA Cleavage; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D012260:Ribonucleases / Q000737:chemistry*
chemical_list: D001427:Bacterial Toxins; D012329:RNA, Bacterial; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D012260:Ribonucleases
keywords: Bacillus anthracis; MoxT; RNA sequence; Ribonuclease