title: Near infrared spectroscopic (NIRS) analysis of drug-loading rate and particle size of risperidone microspheres by improved chemometric model.
abstract: Microspheres have been developed as drug carriers in controlled drug delivery systems for years. In our present study, near infrared spectroscopy (NIRS) is applied to analyze the particle size and drug loading rate in risperidone poly(d,l-lactide-co-glycolide) (PLGA) microspheres. Various batches of risperidone PLGA microspheres were designed and prepared successfully. The particle size and drug-loading rate of all the samples were determined by a laser diffraction particle size analyzer and high performance liquid chromatography (HPLC) system. Monte Carlo algorithm combined with partial least squares (MCPLS) method was applied to identify the outliers and choose the numbers of calibration set. Furthermore, a series of preprocessing methods were performed to remove signal noise in NIR spectra. Moving window PLS and radical basis function neural network (RBFNN) methods were employed to establish calibration model. Our data demonstrated that PLS-developed model was only suitable for drug loading analysis in risperidone PLGA microspheres. Comparatively, RBFNN-based predictive models possess better fitting quality, predictive effect, and stability for both drug loading rate and particle size analysis. The correlation coefficients of calibration set (Rc(2)) were 0.935 and 0.880, respectively. The performance of optimum RBFNN models was confirmed by independent verification test with 15 samples. Collectively, our method is successfully performed to monitor drug-loading rate and particle size during risperidone PLGA microspheres preparation.
authors: School of Life Sciences, Jilin University, No. 2699, Qianjin Avenue, Changchun, Jilin, China.
authors: Jia
authors: J
authors: Song
authors: School of Life Sciences, Jilin University, No. 2699, Qianjin Avenue, Changchun, Jilin, China.
authors: Jing
authors: J
authors: Xie
authors: School of Life Sciences, Jilin University, No. 2699, Qianjin Avenue, Changchun, Jilin, China.
authors: Chenliang
authors: C
authors: Li
authors: School of Life Sciences, Jilin University, No. 2699, Qianjin Avenue, Changchun, Jilin, China.
authors: Jia-Hui
authors: JH
authors: Lu
authors: School of Life Sciences, Jilin University, No. 2699, Qianjin Avenue, Changchun, Jilin, China.
authors: Qing-Fan
authors: QF
authors: Meng
authors: Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH, USA.
authors: Zhaogang
authors: Z
authors: Yang
authors: School of Life Sciences, Jilin University, No. 2699, Qianjin Avenue, Changchun, Jilin, China; Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH, USA.
authors: Robert J
authors: RJ
authors: Lee
authors: School of Life Sciences, Jilin University, No. 2699, Qianjin Avenue, Changchun, Jilin, China. Electronic address: jluwangdi@gmail.com.
authors: Di
authors: D
authors: Wang
authors: School of Life Sciences, Jilin University, No. 2699, Qianjin Avenue, Changchun, Jilin, China. Electronic address: tenglesheng@jlu.edu.cn.
authors: Le-Sheng
authors: LS
authors: Teng
mesh_terms: D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D019344:Lactic Acid / Q000737:chemistry; D016018:Least-Squares Analysis; D008863:Microspheres; D008962:Models, Theoretical; D016571:Neural Networks, Computer; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D018967:Risperidone / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared
chemical_list: D004337:Drug Carriers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D018967:Risperidone
keywords: Microspheres; Near infrared spectroscopy; Partial least squares; Radical basis function neural network; Risperidone