title: [Post-transcriptional regulation of dual-specificity phosphatase-1 by RNA-binding protein HuR T118 in heat shock].
abstract: OBJECTIVE
  To investigate the post-transcriptional regulation of dual-specificity phosphatase-1 (DUSP1) by the RNA- binding protein HuR in heat shock.
  
  
  METHODS
  The recombinant plasmids carrying wild-type (WT) HuR or its mutants at threonine 118 were constructed and transiently transfected into NIH 3T3 cells via liposome, and the changes in the expressions of DUSP1 mRNA and protein were detected by quantitative real-time PCR and Western blotting, respectively.
  
  
  RESULTS
  Heat shock caused significantly enhanced phosphorylation of HuR at the residue T118. In 3T3 cells transfected with the plasmids carrying wild-type HuR for its over-expression showed significantly up-regulated DUSP1 mRNA and protein expressions at 24 h after transfection. Over-expression of HuR(T118A) down-regulated DUSP1 mRNA and protein expressions in cells challenged with heat shock, while HuR(T118E) over-expression significantly increased DISP1 expression at both mRNA and protein levels. After heat shock, HuR(WT) translocated from the cell nucleus to the cytoplasm to form particles. HuR(T118E) was diffusely distributed in the cytoplasm before heat shock and formed particles after heat shock. HuR(T118A) did not undergo such translocation in response to heat shock challenge.
  
  
  CONCLUSIONS
  HuR regulates DUSP1 mRNA and protein expression at the post-transcriptional level to increase its expression after heat shock by enhancing the phosphorylation HuR T118.
authors: Department of Pathophysiology and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou 510515, China.E-mail: zchuanli123@163.com.
authors: Chuanli
authors: C
authors: Zhang
authors: Haihua
authors: H
authors: Luo
authors: Yong
authors: Y
authors: Jiang
mesh_terms: D000818:Animals; D002467:Cell Nucleus; D003593:Cytoplasm; D054638:Dual Specificity Phosphatase 1 / Q000235:genetics / Q000378:metabolism*; D051959:ELAV Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D018869:Heat-Shock Response; D006358:Hot Temperature; D051379:Mice; D041681:NIH 3T3 Cells; D010766:Phosphorylation; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D015854:Up-Regulation
chemical_list: D051959:ELAV Proteins; D012333:RNA, Messenger; D054638:Dual Specificity Phosphatase 1; C517020:Dusp1 protein, mouse