title: IL-4 receptor α in non-lipid rafts is the target molecule of strictinin in inhibiting STAT6 activation.
abstract: Strictinin has been shown to suppress interleukin (IL)-4-induced signal transducer and activator of transcription (STAT)-6 phosphorylation, which is a critical event for IgE class switching. However, it is unclear how strictinin inhibits STAT6 activation. Strictinin inhibited STAT6 phosphorylation by suppressing IL-4 receptor α (IL-4Rα) activation. Strictinin was bound to the cell surface and only localized in non-lipid raft fraction of the cells where IL-4Rα was also located. In addition, strictinin directly bound to IL-4Rα and inhibited binding of IL-4 to IL-4Rα. These results suggest that IL-4Rα locating in non-lipid raft region is a target molecule for strictinin in inhibiting STAT6 activation.
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Yoon Hee
authors: YH
authors: Kim
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Yu
authors: Y
authors: Ninomiya
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Shuya
authors: S
authors: Yamashita
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Motofumi
authors: M
authors: Kumazoe
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Yuhui
authors: Y
authors: Huang
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Kanami
authors: K
authors: Nakahara
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Yeong Seon
authors: YS
authors: Won
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Motoki
authors: M
authors: Murata
authors: Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka 812-8582, Japan.
authors: Yoshinori
authors: Y
authors: Fujimura
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Koji
authors: K
authors: Yamada
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan; Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka 812-8582, Japan; Food Functional Design Research Center, Kyushu University, Fukuoka 812-8581, Japan. Electronic address: tatibana@agr.kyushu-u.ac.jp.
authors: Hirofumi
authors: H
authors: Tachibana
mesh_terms: D000818:Animals; D002051:Burkitt Lymphoma; D006367:HeLa Cells; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D010636:Phenols / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D019948:Receptors, Interleukin-4 / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D050800:STAT6 Transcription Factor / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: C508598:IL4 protein, human; D010636:Phenols; D019948:Receptors, Interleukin-4; D050800:STAT6 Transcription Factor; C494101:STAT6 protein, human; C496786:strictinin; D015847:Interleukin-4
keywords: IgE; Interleukin-4 receptor; Lipid rafts; Molecular target; Strictinin