title: Development of sustained antimicrobial-release systems using poly(2-hydroxyethyl methacrylate)/trimethylolpropane trimethacrylate hydrogels.
abstract: Reconstructive materials with sustained antimicrobial effects could be useful for preventing infectious diseases in an environment containing indigenous bacteria or fungi such as the oral cavity. With the objective of applying a non-biodegradable hydrogel to resin-based materials as a reservoir for water-soluble antimicrobials, novel hydrogels consisting of 2-hydroxyethyl methacrylate (HEMA) and trimethylolpropane trimethacrylate (TMPT) were fabricated. Cetylpyridinium chloride (CPC) was loaded into five hydrogels comprising different ratios of HEMA/TMPT, and their ability to release as well as to be recharged with CPC was examined in vitro. A polyHEMA/TMPT hydrogel comprising 50% HEMA/50% TMPT could be effectively loaded and recharged with CPC by immersion into a CPC solution, demonstrating the longest release of CPC, above the concentration required to inhibit bacteria and fungi. The binding of CPC to the hydrogels was mainly through hydrophobic interaction. Loading of CPC into a hydrogel by mixing CPC powder with the HEMA/TMPT monomer before polymerization resulted in marked extension of the initial CPC-release period. The CPC-pre-mixed hydrogel was confirmed to exhibit antibacterial activity by agar diffusion tests. It is possible to achieve a sustained release system for antimicrobials by pre-mix loading and recharging CPC into a 50% HEMA/50% TMPT hydrogel.
authors: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors: Haruaki
authors: H
authors: Kitagawa
authors: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors: Kahoru
authors: K
authors: Takeda
authors: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors: Ranna
authors: R
authors: Kitagawa
authors: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors: Naomi
authors: N
authors: Izutani
authors: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors: Saeki
authors: S
authors: Miki
authors: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan; Department of Biomaterials Science, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors: Nanako
authors: N
authors: Hirose
authors: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors: Mikako
authors: M
authors: Hayashi
authors: Department of Biomaterials Science, Osaka University Graduate School of Dentistry, Osaka, Japan. Electronic address: imazato@dent.osaka-u.ac.jp.
authors: Satoshi
authors: S
authors: Imazato
mesh_terms: D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000254:growth & development*; D002176:Candida albicans / Q000254:growth & development*; D002594:Cetylpyridinium / Q000737:chemistry / Q000494:pharmacology; D003692:Delayed-Action Preparations; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology; D011409:Propylene Glycols / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000891:Anti-Infective Agents, Local; D003692:Delayed-Action Preparations; D020100:Hydrogels; D008689:Methacrylates; D011409:Propylene Glycols; C018163:trimethylolpropane; C005044:hydroxyethyl methacrylate; D002594:Cetylpyridinium
keywords: Antimicrobial; Drug release; Hydrogel; Resin-based material