title: Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis.
abstract: Gastric cancer is the second leading cause of cancer-related death worldwide. Herein, we investigated the role of transcription factor Yin Yang 1 (YY1), a multi-functional protein, in tumorigenesis of gastric cancer cells. Results showed that YY1 contributed to gastric carcinogenesis of SC-M1 cells including growth, viability, and abilities of colony formation, migration, invasion, and tumorsphere formation. Levels of pluripotency genes CD44, Oct4, SOX-2, and Nanog were also up-regulated by YY1 in SC-M1 cells. Additionally, the 3'-untranslated region (3'-UTR) of YY1 mRNA was the target of microRNA-34 (miR-34) family consisting of miR-34a, miR-34b, and miR-34c. Overexpression of miR-34 family suppressed carcinogenesis through down-regulation of YY1 in NUGC-3 gastric cancer cells scarcely expressing miR-34 family. Alternatively, knockdown of miR-34 family promoted tumorigenesis via up-regulation of YY1 in SC-M1 and AZ521 gastric cancer cells with higher levels of miR-34 family. The miR-34 family also affected tumorsphere ultra-structure and inhibited the xenografted tumor growth as well as lung metastasis of SC-M1 cells through YY1. Expressions of miR-34a and miR-34c in gastric cancer tissues of patients were lower than those in normal tissues. Taken together, these results suggest that miR-34 family-YY1 axis plays an important role in the control of gastric carcinogenesis.
authors: Institute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei , Taiwan. Department of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei , Taiwan.
authors: An-Ming
authors: AM
authors: Wang
authors: Department of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei , Taiwan. Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei , Taiwan. These authors contributed equally to this work.
authors: Tzu-Ting
authors: TT
authors: Huang
authors: Department of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei , Taiwan. These authors contributed equally to this work.
authors: Kai-Wen
authors: KW
authors: Hsu
authors: Department of Surgery, Taipei Veterans General Hospital, Taipei , Taiwan. Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei , Taiwan. These authors contributed equally to this work.
authors: Kuo-Hung
authors: KH
authors: Huang
authors: Department of Surgery, Taipei Veterans General Hospital, Taipei , Taiwan. Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei , Taiwan. These authors contributed equally to this work.
authors: Wen-Liang
authors: WL
authors: Fang
authors: Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei , Taiwan.
authors: Muh-Hwa
authors: MH
authors: Yang
authors: Department of Medicine, School of Medicine, National Yang-Ming University, Taipei , Taiwan. Department of Surgery, National Yang-Ming University Hospital, Yi-Lan, Taiwan.
authors: Su-Shun
authors: SS
authors: Lo
authors: Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei , Taiwan. Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei , Taiwan.
authors: Chin-Wen
authors: CW
authors: Chi
authors: Institute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei , Taiwan. Institute of Biochemistry and Molecular Biology, National Taiwan University College of Medicine, Taipei, Taiwan.
authors: Jing-Jer
authors: JJ
authors: Lin
authors: Department of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei , Taiwan. Genome Research Center, National Yang-Ming University, Taipei , Taiwan. Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.
authors: Tien-Shun
authors: TS
authors: Yeh
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism; D047368:Tumor Burden / Q000235:genetics; D023041:Xenograft Model Antitumor Assays / Q000379:methods; D050986:YY1 Transcription Factor / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D050986:YY1 Transcription Factor; C494698:YY1 protein, human