title: Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats.
abstract: Dietary effects of organic Se supplementation in the form of Se-enriched Agaricus bisporus mushroom on ileal mucosal permeability and antioxidant selenoenzymes status in heat induced oxidative stress in rats were evaluated. Acute heat stress (40 °C, 21% relative humidity, 90 min exposure) increased ileum baseline short circuit current (Isc; 2.40-fold) and epithelial conductance (Ge; 2.74-fold). Dietary supplementation with Se-enriched A. bisporus (1 µg Se/g feed) reduced (p < 0.05) ileum Isc and Ge during heat stress to 1.74 and 1.91 fold, respectively, indicating protection from heat stress-induced mucosal permeability increase. The expression of ileum glutathione peroxidase (GPx-) 1 and 2 mRNAs were up-regulated (p < 0.05) by 1.90 and 1.87-fold, respectively, for non-heat stress rats on the Se-enriched diet relative to the control. The interplay between heat stress and dietary Se is complex. For rats on the control diet, heat stress alone increased ileum expression of GPx-1 (2.33-fold) and GPx-2 (2.23-fold) relative to thermoneutral conditions. For rats on the Se-enriched diet, heat stress increased (p < 0.05) GPx-1 expression only. Rats on Se-enriched + α-tocopherol diet exhibited increased expression of both genes (p < 0.05). Thus, dietary Se-enriched A. bisporus protected against increase in ileum permeability and up-regulated GPx-1 and GPx-2 expression, selenoenzymes relevant to mitigating oxidative stress.
authors: Department of Agriculture and Food Systems, Melbourne School of Land & Environment, Building 142, Parkville Campus, The University of Melbourne, Parkville, Victoria 3010, Australia. t.maseko@student.unimelb.edu.au.
authors: Tebo
authors: T
authors: Maseko
authors: Department of Agriculture and Food Systems, Melbourne School of Land & Environment, Building 142, Parkville Campus, The University of Melbourne, Parkville, Victoria 3010, Australia. fdunshea@unimelb.edu.au.
authors: Frank Rowland
authors: FR
authors: Dunshea
authors: Department of Agriculture and Food Systems, Melbourne School of Land & Environment, Building 142, Parkville Campus, The University of Melbourne, Parkville, Victoria 3010, Australia. khowell@unimelb.edu.au.
authors: Kate
authors: K
authors: Howell
authors: Department of Anatomy & Neuroscience, Melbourne Medical School, Building 181, Parkville Campus, The University of Melbourne, Parkville, Victoria 3010, Australia. hcho@unimelb.edu.au.
authors: Hyun-Jung
authors: HJ
authors: Cho
authors: Department of Anatomy & Neuroscience, Melbourne Medical School, Building 181, Parkville Campus, The University of Melbourne, Parkville, Victoria 3010, Australia. leni.rivera@unimelb.edu.au.
authors: Leni Rose
authors: LR
authors: Rivera
authors: Department of Anatomy & Neuroscience, Melbourne Medical School, Building 181, Parkville Campus, The University of Melbourne, Parkville, Victoria 3010, Australia. j.furness@unimelb.edu.au.
authors: John Barton
authors: JB
authors: Furness
authors: Department of Agriculture and Food Systems, Melbourne School of Land & Environment, Building 142, Parkville Campus, The University of Melbourne, Parkville, Victoria 3010, Australia. ngkf@unimelb.edu.au.
authors: Ken
authors: K
authors: Ng
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature / Q000009:adverse effects; D007422:Intestines / Q000187:drug effects* / Q000502:physiology; D008297:Male; D018384:Oxidative Stress / Q000502:physiology; D010539:Permeability; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*; D015854:Up-Regulation; D000094765:Glutathione Peroxidase GPX1
chemical_list: D005979:Glutathione Peroxidase; C525844:glutathione peroxidase 2, rat; D012643:Selenium; D000094765:Glutathione Peroxidase GPX1