title: Recapitulation of the forward nuclear auxin response pathway in yeast.
abstract: Auxin influences nearly every aspect of plant biology through a simple signaling pathway; however, it remains unclear how much of the diversity in auxin effects is explained by variation in the core signaling components and which properties of these components may contribute to diversification in response dynamics. Here, we recapitulated the entire Arabidopsis thaliana forward nuclear auxin signal transduction pathway in Saccharomyces cerevisiae to test whether signaling module composition enables tuning of the dynamic response. Sensitivity analysis guided by a small mathematical model revealed the centrality of auxin/indole-3-acetic acid (Aux/IAA) transcriptional corepressors in controlling response dynamics and highlighted the strong influence of natural variation in Aux/IAA degradation rates on circuit performance. When the basic auxin response circuit was expanded to include multiple Aux/IAAs, we found that dominance relationships between coexpressed Aux/IAAs were sufficient to generate distinct response modules similar to those seen during plant development. Our work provides a new method for dissecting auxin signaling and demonstrates the key role of Aux/IAAs in tuning auxin response dynamics.
authors: Departments of Biology and.
authors: Edith
authors: E
authors: Pierre-Jerome
authors: Electrical Engineering, University of Washington, Seattle, WA 98195.
authors: Seunghee S
authors: SS
authors: Jang
authors: Electrical Engineering, University of Washington, Seattle, WA 98195.
authors: Kyle A
authors: KA
authors: Havens
authors: Departments of Biology and jn7@uw.edu klavins@uw.edu.
authors: Jennifer L
authors: JL
authors: Nemhauser
authors: Electrical Engineering, University of Washington, Seattle, WA 98195 jn7@uw.edu klavins@uw.edu.
authors: Eric
authors: E
authors: Klavins
mesh_terms: D017360:Arabidopsis / Q000378:metabolism / Q000502:physiology*; D005434:Flow Cytometry; D005822:Genetic Vectors / Q000235:genetics; D007210:Indoleacetic Acids / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D012441:Saccharomyces cerevisiae; D015398:Signal Transduction / Q000502:physiology*; D058615:Synthetic Biology
chemical_list: D007210:Indoleacetic Acids; C030737:indoleacetic acid
keywords: signaling dynamics; synthetic biology