title: Microevolution of Burkholderia pseudomallei during an acute infection.
abstract: We used whole-genome sequencing to evaluate 69 independent colonies of Burkholderia pseudomallei isolated from seven body sites of a patient with acute disseminated melioidosis. Fourteen closely related genotypes were found, providing evidence for the rapid in vivo diversification of B. pseudomallei after inoculation and systemic spread.
authors: Department of Tropical Medicine and Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand direk@tropmedres.ac.
authors: Direk
authors: D
authors: Limmathurotsakul
authors: The Wellcome Trust Sanger Institute, Cambridge, United Kingdom.
authors: Matthew T G
authors: MT
authors: Holden
authors: The Wellcome Trust Sanger Institute, Cambridge, United Kingdom.
authors: Paul
authors: P
authors: Coupland
authors: Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia.
authors: Erin P
authors: EP
authors: Price
authors: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors: Narisara
authors: N
authors: Chantratita
authors: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors: Vanaporn
authors: V
authors: Wuthiekanun
authors: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors: Premjit
authors: P
authors: Amornchai
authors: The Wellcome Trust Sanger Institute, Cambridge, United Kingdom.
authors: Julian
authors: http://orcid.org/0000-0002-7069-5958
authors: J
authors: Parkhill
authors: Department of Medicine, Cambridge University, Addenbrooke's Hospital, Cambridge, United Kingdom.
authors: Sharon J
authors: SJ
authors: Peacock
mesh_terms: D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D014644:Genetic Variation; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D008297:Male; D008554:Melioidosis / Q000382:microbiology*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial