title: Assessing morphokinetic parameters via time lapse microscopy (TLM) to predict euploidy: are aneuploidy risk classification models universal?
abstract: OBJECTIVE
  To determine if Aneuploidy Risk Classification Models are predictive of euploidy/aneuploidy amongst IVF facilities.
  
  
  METHODS
  We retrospectively applied key time lapse imaging events of embryos (Campbell et al.[5, 6]) to stratify embryos into 3 groups: low, medium and high risk of aneuploidy. The actual ploidy results (from array comparative genomic hybridization) were compared with expectations [5, 6]. Sources of variability in morphokinetic parameters were determined using Analysis of Variance (ANOVA).
  
  
  RESULTS
  The model failed to segregate euploid embryos from aneuploid embryos cultured at our facility. Further analysis indicated that the variability of embryos among patients was too great to allow selection of euploid embryos based on simple morphokinetic thresholds. Clinical selection of embryos based on morphokinetics alone is unlikely to identify euploid embryos accurately for transfer or yield higher rates of live delivery.
  
  
  CONCLUSIONS
  The use of non-invasive morphokinetics is unlikely to discriminate aneuploid from euploid embryos. Further, it does not approach the accuracy of preimplantation genetic screening with array comparative genomic hybridization.
authors: NYU Fertility Center, NYU Langone School of Medicine, 660 First Avenue, 5th floor, New York, NY, 10016, USA, yael.kramer@nyumc.org.
authors: Yael G
authors: YG
authors: Kramer
authors: Jason D
authors: JD
authors: Kofinas
authors: Katherine
authors: K
authors: Melzer
authors: Nicole
authors: N
authors: Noyes
authors: Caroline
authors: C
authors: McCaffrey
authors: Julia
authors: J
authors: Buldo-Licciardi
authors: David H
authors: DH
authors: McCulloh
authors: James A
authors: JA
authors: Grifo
mesh_terms: D000704:Analysis of Variance; D000782:Aneuploidy; D055028:Comparative Genomic Hybridization; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D019836:Preimplantation Diagnosis / Q000379:methods*; D012189:Retrospective Studies; D059008:Time-Lapse Imaging