title: The lack of evidence for correlation of pyruvate kinase M2 expression with tumor grade in non-small cell lung cancer.
abstract: BACKGROUND
  Over the last years, evidence has accumulated that an increased expression of pyruvate kinase M2 isozyme (PKM2) is related to neoplastic transformation as well that its plasma concentrations might be a marker of lung cancer progression.
  
  
  METHODS
  In the present manuscript an immunohistochemical technique was used to detect the expression of two pyruvate kinase isoforms: PKM1 (muscle isozyme of PK) and PKM2 as well Ki-67 antigen on paraffin sections of 218 cases of non-small cell lung cancer (NSCLC) of different histological types and grades of malignancy.
  
  
  RESULTS
  A significant correlation between expressions of both pyruvate kinase isoforms in all NSCLC types was found (r=0.42, p<0.0001). Expression levels of PKM1 and PKM2 were independent of the histological classification of the tumor and patients' clinicopathological data.
  
  
  CONCLUSIONS
  PKM2 and PKM1 have no value as predictive markers of NSCLC regardless of the histological type and grade of malignancy.
authors: Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland.
authors: Christopher
authors: C
authors: Kobierzycki
authors: Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland.
authors: Bartosz
authors: B
authors: Pula
authors: Department of Pulmonology and Pulmonary Tumours, Wroclaw Medical University, Wroclaw, Poland.
authors: Bozena
authors: B
authors: Werynska
authors: Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland.
authors: Aleksandra
authors: A
authors: Piotrowska
authors: Laboratory of Histopathology, Lower Silesian Centre of Pulmonary Diseases, Wroclaw, Poland.
authors: Beata
authors: B
authors: Muszczynska-Bernhard
authors: Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland Department of Physiotherapy, University School of Physical Education, Wroclaw, Poland.
authors: Piotr
authors: P
authors: Dziegiel
authors: Department of Animal Molecular Physiology, Institute of Experimental Biology, Wroclaw University, Wroclaw, Poland drakus@biol.uni.wroc.pl.
authors: Dariusz
authors: D
authors: Rakus
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology* / Q000473:pathology*; D002352:Carrier Proteins / Q000096:biosynthesis*; D003593:Cytoplasm / Q000201:enzymology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D008875:Middle Aged; D060787:Neoplasm Grading; D016612:Paraffin Embedding; D011379:Prognosis; D013963:Thyroid Hormones / Q000096:biosynthesis*; D000097810:Thyroid Hormone-Binding Proteins
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D019394:Ki-67 Antigen; D008565:Membrane Proteins; D013963:Thyroid Hormones; D000097810:Thyroid Hormone-Binding Proteins
keywords: Non-small cell lung cancer; immunohistochemistry; pyruvate kinase