title: Comparison of effects of inhibitors of viral and cellular protein kinases on human cytomegalovirus disruption of nuclear lamina and nuclear egress.
abstract: Human cytomegalovirus (HCMV) kinase UL97 is required for efficient nuclear lamina disruption during nuclear egress. However, cellular protein kinase C (PKC) has been implicated in this process in other systems. Comparing the effects of UL97 and cellular kinase inhibitors on HCMV nuclear egress confirms a role for UL97 in lamina disruption and nuclear egress. A pan-PKC inhibitor did not affect lamina disruption but did reduce the number of cytoplasmic capsids more than the number of nuclear capsids.
authors: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
authors: Mayuri
authors: M
authors: Sharma
authors: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA don_coen@hms.harvard.edu.
authors: Donald M
authors: DM
authors: Coen
mesh_terms: D002213:Capsid / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000821:virology*; D003587:Cytomegalovirus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D003586:Cytomegalovirus Infections / Q000201:enzymology* / Q000821:virology; D006801:Humans; D034881:Nuclear Lamina / Q000187:drug effects / Q000821:virology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D019065:Virus Assembly / Q000187:drug effects; D057074:Virus Release / Q000187:drug effects*
chemical_list: D047428:Protein Kinase Inhibitors; D017853:Phosphotransferases (Alcohol Group Acceptor); C075365:ganciclovir kinase; D011493:Protein Kinase C