title: Analysis of the epithelial damage produced by Entamoeba histolytica infection.
abstract: Entamoeba histolytica is the causative agent of human amoebiasis, a major cause of diarrhea and hepatic abscess in tropical countries. Infection is initiated by interaction of the pathogen with intestinal epithelial cells. This interaction leads to disruption of intercellular structures such as tight junctions (TJ). TJ ensure sealing of the epithelial layer to separate host tissue from gut lumen. Recent studies provide evidence that disruption of TJ by the parasitic protein EhCPADH112 is a prerequisite for E. histolytica invasion that is accompanied by epithelial barrier dysfunction. Thus, the analysis of molecular mechanisms involved in TJ disassembly during E. histolytica invasion is of paramount importance to improve our understanding of amoebiasis pathogenesis. This article presents an easy model that allows the assessment of initial host-pathogen interactions and the parasite invasion potential. Parameters to be analyzed include transepithelial electrical resistance, interaction of EhCPADH112 with epithelial surface receptors, changes in expression and localization of epithelial junctional markers and localization of parasite molecules within epithelial cells.
authors: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute; abigailbetanzos@gmail.com.
authors: Abigail
authors: A
authors: Betanzos
authors: Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute.
authors: Michael
authors: M
authors: Schnoor
authors: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute.
authors: Rosario
authors: R
authors: Javier-Reyna
authors: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute.
authors: Guillermina
authors: G
authors: García-Rivera
authors: Agency for Knowledge Commercialization, Center for Research and Advanced Studies of the National Polytechnic Institute.
authors: Cecilia
authors: C
authors: Bañuelos
authors: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute.
authors: Jonnatan
authors: J
authors: Pais-Morales
authors: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute; orozco.esther@gmail.com.
authors: Esther
authors: E
authors: Orozco
mesh_terms: D000818:Animals; D004285:Dogs; D004748:Entamoeba histolytica / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D004749:Entamoebiasis / Q000469:parasitology / Q000473:pathology*; D004847:Epithelial Cells / Q000469:parasitology* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D054884:Host-Pathogen Interactions; D061985:Madin Darby Canine Kidney Cells; D018613:Microscopy, Confocal / Q000379:methods; D011480:Protease Inhibitors / Q000494:pharmacology; D015800:Protozoan Proteins / Q000378:metabolism
chemical_list: D011480:Protease Inhibitors; D015800:Protozoan Proteins