title: Total phenolic and flavonoid contents, antioxidant and antimicrobial activities of Alnus glutinosa (L.) Gaertn., Alnus incana (L.) Moench and Alnus viridis (Chaix) DC. extracts.
abstract: The objective of this study was to determine total phenolic and flavonoid contents, antioxidant and antimicrobial activities of methanolic extracts from the leaves and barks of three Alnus species. The phenolic and flavonoid contents of extracts were determined spectrophotometrically using Folin-Ciocalteau and aluminium chloride methods, respectively. In addition, antioxidant activity of the extracts was determined using 1,1-diphenyl-2-picrylhydrazyl radical scavenging method. The antimicrobial activity was performed by disc diffusion assay against six reference bacterial strains including Gram-negative and Gram-positive bacteria and two fungal strains. Extract of Alnus viridis bark contained the highest amounts of total phenolics (780 mg CAT/g), while extract of A. viridis leaves had the highest amount of flavonoids (30.01 mg RUT/g). All extracts showed antioxidant activity higher than thymol, which was used as a positive probe. The largest diameters of inhibition zone (25 mm) were recorded with Bacillus subtilis 168 M and Staphylococcus aureus ATCC 6538.
authors: a Department of Biology , Faculty of Science, University of Sarajevo , Sarajevo , Bosnia and Herzegovina.
authors: Sabina
authors: S
authors: Dahija
authors: Jasmina
authors: J
authors: Cakar
authors: Danijela
authors: D
authors: Vidic
authors: Milka
authors: M
authors: Maksimović
authors: Adisa
authors: A
authors: Parić
mesh_terms: D029661:Alnus / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010851:Picrates / Q000494:pharmacology; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts; D018515:Plant Leaves / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C011471:folin; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Alnus; antibacterial activity; antifungal activity; antioxidant activity; flavonoids; phenolics