title: [Amphotericin B suppresses migration and invasion of esophageal carcinoma Eca109 cells in hypoxic microenvironment by down-regulating hypoxia-inducible factor-1α activity].
abstract: OBJECTIVE
  To investigate the effect of amphotericinB (AmB) on migration and invasion of esophageal carcinoma Eca109 cells exposed to hypoxia and explore the molecular mechanisms.
  
  
  METHODS
  Routinely cultured esophageal carcinoma Eca109 cells were treated with 0, 1.25, 2.5, or 5 µg/ml AmB in hypoxic condition (3% O2, 5% CO2, and 92% N2) for 24 h. The cell migration and invasion were assessed by cell scratch test and Transwell chamber assay, respectively. Real-time quantitative PCR and Western blotting were used to detect the mRNA and protein expressions of hypoxia-inducible factor-1α (HIF-1α), matrix metalloproteinase-2 (MMP-2), and E-cadherin in the cells, respectively.
  
  
  RESULTS
  Compared with the control cells, the cells treated with different doses of AmB showed attenuated ability of migration and invasion (P<0.05). AmB treatment resulted in significantly lowered mRNA and protein expressions of MMP-2 (P<0.05) and increased expressions of E-cadherin (P<0.05); the protein expression of HIF-1α decreased significantly in cells after AmB treatment (P<0.05) but its mRNA levels showed no significant changes (P>0.05).
  
  
  CONCLUSIONS
  AmB can suppress the migration and invasion of esophageal carcinoma Eca109 cells in hypoxic microenvironment possibly by regulating the expressions of HIF-1α, MMP-2 and E-cadherin.
authors: Department of Radiology and Oncology, Chongqing Medical University, Chongqing 400016, China.E-mail: hua_bujie@163.com.
authors: Qiangqiang
authors: Q
authors: Kang
authors: Min
authors: M
authors: Tang
authors: Yanli
authors: Y
authors: Hou
authors: Liqun
authors: L
authors: Duan
authors: Xingyue
authors: X
authors: Chen
authors: Jin
authors: J
authors: Shu
authors: Furong
authors: F
authors: Wu
authors: Ying
authors: Y
authors: Wang
authors: Shaolin
authors: S
authors: Li
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism*; D015687:Cell Hypoxia; D045744:Cell Line, Tumor / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D012333:RNA, Messenger
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D000666:Amphotericin B; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2