title: CD24 regulates stemness and the epithelial to mesenchymal transition through modulation of Notch1 mRNA stability by p38MAPK.
abstract: We report here that CD24 knockdown resulted in decreased expression of Notch1 in MCF-7 cells. CD24-downstream p38MAPK was shown to regulate Notch1 at the level of mRNA stability. We also found that CD24-mediated cell migration, invasion, mammosphere formation, and drug resistance was regulated by its downstream target Notch1. Together, our results indicate that CD24 may regulate the epithelial to mesenchymal transition and stemness through Notch1 signaling in breast cancer cells.
authors: Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea.
authors: Juyeon
authors: J
authors: Lim
authors: Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea.
authors: Kyung-min
authors: KM
authors: Lee
authors: Department of Molecular Biology, Sejong University, Seoul 143-747, Republic of Korea.
authors: Jaekyung
authors: J
authors: Shim
authors: Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea; Natural Science Institute, Hanyang University, Seoul 133-791, Republic of Korea. Electronic address: incheol@hanyang.ac.kr.
authors: Incheol
authors: I
authors: Shin
mesh_terms: D051927:CD24 Antigen / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition; D055785:Gene Knockdown Techniques; D006801:Humans; D061986:MCF-7 Cells; D014411:Neoplastic Stem Cells / Q000473:pathology*; D010641:Phenotype; D020871:RNA Stability; D012333:RNA, Messenger / Q000737:chemistry; D051881:Receptor, Notch1 / Q000235:genetics*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D051927:CD24 Antigen; D012333:RNA, Messenger; D051881:Receptor, Notch1; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Breast cancer; CD24; CSC; EMT; Notch1