title: Identification of Microbial Pathogens in Periodontal disease and Diabetic patients of South Indian Population.
abstract: Periodontitis have been referred to as the sixth complication of diabetes found in high prevalence among diabetic patients than among healthy controls. The aim of the present study was to examine the periodontal disease status among collected dental plaque samples. Chromosomal DNA was isolated and amplified by universal primers. The DNA was sequenced for bacterial confirmation and phylogenetic analysis performed for the evolutionary relationship with other known pathogens. No amplification products were observed in groups labeled non periodontal and non Diabetes (NP&ND) and non Periodontal and Diabetes (NP&D). But in the case of Periodontal and non Diabetes (P&ND) groups 22 amplified products were observed. In case of Periodontal and Diabetes (P&D), 32 amplified products were positive for microbes. Among the four microbial groups, Treponemadenticola, and Tannerella forsythia were found to be prevalent in P&D. The phylogenetic analysis of 16s rRNA of Treponemadenticola showed the relationship with other Treponema oral pathogen species and with the Spirochaetazuelaera. Tannerella forsythia shows its evolutionary relationship only with four oral pathogens (Macellibacteroidesfermentans, Porphyromadaceae bacterium, Parabacteroidesmeredae and Bacillus fosythus). Prevotellaintermedia also showed its evolutionary relationship only with Prevotella Spcs while Fusobacterium revealed close evolutionary relationship only with Porpiromonasgingivalis.
authors: Division of Animal Biotechnology, Department of Zoology, S. V. University, Tirupati-517502, Andhra Pradesh, India.
authors: Tikka
authors: T
authors: Chiranjeevi
authors: Division of Animal Biotechnology, Department of Zoology, S. V. University, Tirupati-517502, Andhra Pradesh, India.
authors: Osuru Hari
authors: OH
authors: Prasad
authors: Department of Biotechnology, SVIMS, Tirupati-517502, Andhra Pradesh, India.
authors: Uppu Venkateswara
authors: UV
authors: Prasad
authors: Department of Periodontology, Krishna Teja Dental College, Tirupati, Andhra Pradesh, India.
authors: Avula Kishor
authors: AK
authors: Kumar
authors: Division of Animal Biotechnology, Department of Zoology, S. V. University, Tirupati-517502, Andhra Pradesh, India.
authors: Veeraraghavulu Praveen
authors: VP
authors: Chakravarthi
authors: Division of Animal Biotechnology, Department of Zoology, S. V. University, Tirupati-517502, Andhra Pradesh, India.
authors: Paramala Balaji
authors: PB
authors: Rao
authors: Department of Biotechnology, SVIMS, Tirupati-517502, Andhra Pradesh, India.
authors: Potuguchi Venkata Gurunadha Krishna
authors: PV
authors: Sarma
authors: Department of Periodontology, Krishna Teja Dental College, Tirupati, Andhra Pradesh, India.
authors: Nagi Reddy Raveendra
authors: NR
authors: Reddy
authors: Division of Animal Biotechnology, Department of Zoology, S. V. University, Tirupati-517502, Andhra Pradesh, India.
authors: Matcha
authors: M
authors: Bhaskar
keywords: Diabetes; Microbial pathogens; PCR; Periodontal diseases