title: Increased survival of human free fat grafts with varying densities of human adipose-derived stem cells and platelet-rich plasma.
abstract: The high absorption rate of transplanted fat has limited the application of autogenous fat grafts in the clinical setting. Therefore, this study aimed to evaluate the effects of platelet-rich plasma (PRP) and adipose-derived stem cells (ASCs) on fat regeneration by investigating the impact of PRP and conditioned medium on the biological characteristics of ASCs. Fat grafts were prepared with ASCs at densities of 107/ml, 106/ml, 105/ml, 104/ml and 0/ml with and without PRP and injected subcutaneously into nude mice. Liquid overflow method, haematoxylin and eosin staining, and immunohistochemical analyses were used to examine the fat grafts. The residual fat volume of the 105/ml ASC + PRP group was significantly higher than that of other treatment conditions after 90 days. Furthermore, histological examination revealed that in 105/ml ASCs-treated grafts normal adipocyte area and capillary formation were increased dramatically compared with other treatment conditions. It is concluded that fat grafts consisting of PRP and 105/ml ASCs constitute an ideal transplant strategy, which may result in decreased absorption and accelerated fat regeneration. This simple and reliable method could provide a valuable and needed tool in plastic and reconstructive surgery. Copyright © 2014 John Wiley & Sons, Ltd.
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu, China.|Department of Oral and Maxillofacial Surgery, School of stomatology, Central South University, Changsha, China.
authors: Kun
authors: K
authors: Li
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu, China.|Department of Oral and Maxillofacial Surgery, School of stomatology, Central South University, Changsha, China.
authors: Feng
authors: F
authors: Li
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors: Jie
authors: J
authors: Li
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu, China.
authors: Hang
authors: H
authors: Wang
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu, China.
authors: Xiaohui
authors: X
authors: Zheng
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu, China.
authors: Jie
authors: J
authors: Long
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Department of Pedodontics, West China School of Stomatology, Sichuan University, Chengdu, China.
authors: Weihua
authors: W
authors: Guo
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu, China.|Department of Oral and Maxillofacial Surgery, School of stomatology, Central South University, Changsha, China.
authors: Weidong
authors: W
authors: Tian
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D017077:Culture Media, Conditioned / Q000737:chemistry; D006085:Graft Survival; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D019651:Plastic Surgery Procedures; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
chemical_list: D017077:Culture Media, Conditioned
keywords: adipose-derived stem cells; angiogenesis; fat regeneration; fat transfer; graft survival; platelet-rich plasma