title: Transitory improvement of articular cartilage characteristics after implantation of polylactide:polyglycolic acid (PLGA) scaffolds seeded with autologous mesenchymal stromal cells in a sheep model of critical-sized chondral defect.
abstract: Clinical translation of emerging technologies aiming at cartilage resurfacing is hindered by neither the appropriate scaffold design nor the optimal cell source having been defined. Here, critical-sized, chondral-only focal defects were created in sheep and treated with clinical-grade, co-polymeric poly-lactide:polyglycolic acid scaffolds either alone or seeded with 3.3 × 10(6) ± 0.4 × 10(6) autologous bone marrow-derived mesenchymal stromal cells and studied over 12 month follow-up. An untreated group was included for comparison. Second-look arthroscopy performed at 4 months post-treatment evidenced the generation of neocartilage of better quality in those defects treated with cells. However, macroscopic scores in the cell-treated group declined significantly from 7.5 ± 2.3 at 4 months to 3.1 ± 2.6 (p = 0.0098) at 12 months post-treatment, whereas the other two experimental groups remained unaltered during 4-12 month post-treatment. The effectiveness of the cell-based approach proposed in this study is thus restricted to between months 1 and 4 post-treatment.
authors: Divisió de Teràpies Avançades/XCELIA, Banc de Sang i Teixits, Edifici Dr. Frederic Duran i Jordà, Passeig Taulat, 116, 08005, Barcelona, Spain.
authors: M
authors: M
authors: Caminal
authors: X
authors: X
authors: Moll
authors: D
authors: D
authors: Codina
authors: R M
authors: RM
authors: Rabanal
authors: A
authors: A
authors: Morist
authors: J
authors: J
authors: Barrachina
authors: F
authors: F
authors: Garcia
authors: A
authors: A
authors: Pla
authors: J
authors: J
authors: Vives
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002357:Cartilage Diseases / Q000378:metabolism / Q000628:therapy*; D002358:Cartilage, Articular / Q000737:chemistry; D004195:Disease Models, Animal; D007719:Knee Joint / Q000473:pathology*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D012756:Sheep; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011100:Polyglycolic Acid; C033616:poly(lactide)