title: [Effect of sodium tanshinone II (A) sulfonate on Ang II -induced atrial fibroblast collagen synthesis and TGF-beta1 activation].
abstract: OBJECTIVE
  To observe the effect of sodium tanshinone II (A) sulfonate (STS) on Ang II -induced atrial fibroblast collagen synthesis and TGF-beta1 activation.
  
  
  METHODS
  Atrial fibroblasts of neonatal rats were cultured to determine the content of collagen protein. The original synthesis rate determined by the [3H]-proline incorporation method was taken as the index for myocardial fibrosis. The content of active TGF-beta1 and total TGF-beta1 in cell culture supernatants were tested and cultured by ELISA. The expression of thrombospondin-1 (TSP-1) was assessed by using Western blot.
  
  
  RESULTS
  Ang II could significantly increase the content of atrial fibroblast collagen and the collagen synthesis rate, the TSP-1 expression and the concentration of active TGF-beta1, without any obvious change in total TGF-beta1. After the STS treatment, all of the indexes, apart from total TGF-beta1, were obviously down-regulated.
  
  
  CONCLUSIONS
  STS could decrease the secretion of Ang II -induced atrial fibroblast collagen and the synthesis rate. Its mechanism is related to the inhibition of TSP-1/TGF-beta1 pathway.
authors: Le
authors: L
authors: Yang
authors: Xiao-Jing
authors: XJ
authors: Zou
authors: Zhao
authors: Z
authors: Yin
authors: Hong-Zhen
authors: HZ
authors: Hao
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006325:Heart Atria / Q000166:cytology*; D010616:Phenanthrenes / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D019700:Thrombospondin 1 / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D010616:Phenanthrenes; D019700:Thrombospondin 1; D053773:Transforming Growth Factor beta1; D000804:Angiotensin II; C024894:tanshinone II A sodium sulfonate; D003094:Collagen