title: Lipopolysaccharides isolated from Aeromonas salmonicida and Vibrio anguillarum show quantitative but not qualitative differences in inflammatory outcome in Sparus aurata (Gilthead seabream).
abstract: In fish, the defence system recognises pathogenic microorganisms via pathogen recognition receptors (PRRs) that sense particular structures of the pathogens; the so-called pathogen associated molecular patterns (PAMPs) such as bacterial lipopolysaccharides (LPSs). The result of the PAMP-PRR interactions leads to complex and orchestrated immune responses. In this study, Sparus aurata (Gilthead seabream) were intraperitoneally injected with purified lipopolysaccharide (LPS) from Aeromonas salmonicida (As)- and Vibrio anguillarum (Va) (1 mg*Kgfish(-1)), both Gram negative bacteria responsible for vibriosis and furunculosis respectively, therefore causing an impact upon marine fish cultures. Head-kidney, intestine, spleen, liver and blood samples were collected at 3, 6, 12 and 24 h post-injection. Plasma levels of cortisol, prostaglandins and lactate were measured and were significantly increased after As-LPS and Va-LPS treatment. Furthermore, tissue-specific differences of the gene regulatory patterns were evident for each LPS. When monocyte/macrophage cell cultures were challenged with As-LPS and Va-LPS, the pro-inflammatory cytokine mRNA abundances present a similar pattern of response. However, As-LPS always triggered a stronger response concerning TNFα, IL1β and cyclooxygenase-2 (COX2) mRNA abundance as well as PGE2 levels in the supernatant. Overall, the results indicate that specific LPSs do not activate different pro-inflammatory responses and that the observed gene expression pattern is tissue and concentration dependent.
authors: Institut de Biotecnologia i de Biomedicina, Universitat Autónoma de Barcelona, Bellaterra, Barcelona 08193, Spain; Institute of Aquaculture, University of Stirling, UK. Electronic address: sebastian.boltana@stir.ac.uk.
authors: S
authors: S
authors: Boltaña
authors: Department of Cell Biology, Physiology and Immunology, Universitat Autónoma de Barcelona, 08193 Barcelona, Spain.
authors: R
authors: R
authors: Tridico
authors: Institut de Biotecnologia i de Biomedicina, Universitat Autónoma de Barcelona, Bellaterra, Barcelona 08193, Spain; Department of Cell Biology, Physiology and Immunology, Universitat Autónoma de Barcelona, 08193 Barcelona, Spain.
authors: M
authors: M
authors: Teles
authors: Institut de Biotecnologia i de Biomedicina, Universitat Autónoma de Barcelona, Bellaterra, Barcelona 08193, Spain; Institute of Aquaculture, University of Stirling, UK.
authors: S
authors: S
authors: Mackenzie
authors: Department of Cell Biology, Physiology and Immunology, Universitat Autónoma de Barcelona, 08193 Barcelona, Spain.
authors: L
authors: L
authors: Tort
mesh_terms: D048409:Aeromonas salmonicida / Q000737:chemistry*; D000704:Analysis of Variance; D000818:Animals; D018929:Cell Culture Techniques; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005393:Fish Diseases / Q000139:chemically induced* / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D006854:Hydrocortisone / Q000097:blood; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000662:veterinary*; D019344:Lactic Acid / Q000097:blood; D008070:Lipopolysaccharides / Q000009:adverse effects* / Q000032:analysis; D011453:Prostaglandins / Q000097:blood; D060888:Real-Time Polymerase Chain Reaction; D021541:Sea Bream; D014733:Vibrio / Q000737:chemistry*
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D011453:Prostaglandins; D019344:Lactic Acid; D006854:Hydrocortisone
keywords: Defence system; Lipopolysaccharide; Sparus aurata