title: Expression of apoptosis mediators p53 and caspase 3, 7, and 9 in chronic rhinosinusitis with nasal polyposis.
abstract: BACKGROUND
  The causal factor for the perpetuation of the inflammatory process in chronic rhinosinusitis with nasal polyps (CRSwNPs) has been extensively studied. However, little is known about the influence of cell death in this disease. Thus, the molecular assessment of mechanisms involved in apoptosis might shed light on the pathogenesis of CRSwNPs. This study was designed to evaluate the gene expression of different apoptotic factors in patients with NPs compared with control patients.
  
  
  METHODS
  The mRNA expression of the apoptosis mediators caspase 3, 7, and 9 and of p53 protein was analyzed using quantitative reverse transcription-polymerase chain reaction in 25 NPs and 18 control samples.
  
  
  RESULTS
  We observed significantly lower expression of p53 and caspase 3 and 9 in patients with CRSwNPs compared with the controls, whereas caspase 7 expression was not significantly different from the controls.
  
  
  CONCLUSIONS
  The reduced expression of these apoptosis factors in CRSwNPs might be related to higher proliferation and the perpetuation of inflammatory cells hindering the control of the disease. A better understanding of the possible influence of apoptosis factors on CRSwNPs could provide rationale for future therapies.
authors: Department of Ophthalmology, Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
authors: Daniel Salgado
authors: DS
authors: Küpper
authors: Fabiana C P
authors: FC
authors: Valera
authors: Rafael
authors: R
authors: Malinsky
authors: Cristiane Maria
authors: CM
authors: Milanezi
authors: João S
authors: JS
authors: Silva
authors: Edwin
authors: E
authors: Tamashiro
authors: Wilma T
authors: WT
authors: Anselmo-Lima
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism*; D053179:Caspase 7 / Q000235:genetics / Q000378:metabolism*; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism*; D002908:Chronic Disease; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000175:diagnosis* / Q000276:immunology; D012220:Rhinitis / Q000175:diagnosis* / Q000276:immunology; D012852:Sinusitis / Q000175:diagnosis* / Q000276:immunology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D016159:Tumor Suppressor Protein p53; D053148:Caspase 3; D053179:Caspase 7; D053453:Caspase 9