title: Growth and differentiation factor 10 (Gdf10) is involved in Bergmann glial cell development under Shh regulation.
abstract: Growth differentiation factor 10 (Gdf10), also known as Bmp3b, is a member of the transforming growth factor (TGF)-ß superfamily. Gdf10 is expressed in Bergmann glial cells, which was investigated by single-cell transcriptional profiling (Koirala and Corfas, (2010) PLoS ONE 5: e9198). Here we provide a detailed characterization of Gdf10 expression from E14, the stage at which Gdf10 is expressed for the first time in the cerebellum, until P28. We detected Gdf10 expression in both germinal zones: in the ventricular zone (VZ) of the 4th ventricle as well as in the rhombic lip (RL). The VZ has been postulated to give rise to GABAergic neurons and glial cells, whereas the RL gives rise to glutamatergic neurons. Thus, it was very surprising to discover a gene that is expressed exclusively in glial cells and is not restricted to an expression in the VZ, but is also present in the RL. At postnatal stages Gdf10 was distributed equally in Bergmann glial cells of the cerebellum. Furthermore, we found Gdf10 to be regulated by Sonic hedgehog (Shh), which is secreted by Purkinje cells of the cerebellum. In the conditional Shh mutants, glial cells showed a reduced expression of Gdf10, whereas the expression of Nestin and Vimentin was unchanged. Thus, we show for the first time, that Gdf10, expressed in Bergmann glial cells, is affected by the loss of Shh as early as E18.5, suggesting a regulation of glial development by Shh.
authors: Instituto de Neurociencias de Alicante, Universidad Miguel Hernandez, Consejo Superior de Investigaciones Científicas (UMH-CSIC), E-03550, Alicante, Spain; Max-Delbrück-Center for Molecular Medicine, D-13125, Berlin, Germany.
authors: Nora
authors: N
authors: Mecklenburg
authors: Jesus E
authors: JE
authors: Martinez-Lopez
authors: Juan Antonio
authors: JA
authors: Moreno-Bravo
authors: Ariadna
authors: A
authors: Perez-Balaguer
authors: Eduardo
authors: E
authors: Puelles
authors: Salvador
authors: S
authors: Martinez
mesh_terms: D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism*; D055413:Growth Differentiation Factor 10 / Q000378:metabolism*; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D064231:Nestin / Q000378:metabolism; D009457:Neuroglia / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014746:Vimentin / Q000378:metabolism
chemical_list: C080330:En1 protein, mouse; C525784:Gdf10 protein, mouse; D055413:Growth Differentiation Factor 10; D053823:Hedgehog Proteins; D018398:Homeodomain Proteins; C577485:Nes protein, mouse; D064231:Nestin; D012333:RNA, Messenger; C465570:Shh protein, mouse; D014746:Vimentin
keywords: Bergmann glia; Bmp3b; Gdf10; Nestin; Shh; cerebellum