title: Identification and characterization of the spiruchostatin biosynthetic gene cluster enable yield improvement by overexpressing a transcriptional activator.
abstract: Spiruchostatins A and B are members of the FK228-family of natural products with potent histone deacetylase inhibitory activities and antineoplastic activities. However, their production in the wild-type strain of Pseudomonas sp. Q71576 is low. To improve the yield, the spiruchostatin biosynthetic gene cluster (spi) was first identified by rapid genome sequencing and characterized by genetic mutations. This spi gene cluster encodes a hybrid biosynthetic pathway similar to that encoded by the FK228 biosynthetic gene cluster (dep) in Chromobacterium violaceum No. 968. Each gene cluster contains a pathway regulatory gene (spiR vs. depR), but these two genes encode transcriptional activators of different classes. Overexpression of native spiR or heterologous depR in the wild-type strain of Pseudomonas sp. Q71576 resulted in 268 or 1,285 % increase of the combined titer of spiruchostatins A and B, respectively. RT-PCR analysis indicates that overexpression of heterologous depR upregulates the expression of native spiR.
authors: Department of Biological Sciences, University of Wisconsin-Milwaukee, PO Box 413, Milwaukee, WI, 53201, USA.
authors: Vishwakanth Y
authors: VY
authors: Potharla
authors: Cheng
authors: C
authors: Wang
authors: Yi-Qiang
authors: YQ
authors: Cheng
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways; D002861:Chromobacterium / Q000235:genetics / Q000378:metabolism*; D047630:Depsipeptides / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D011549:Pseudomonas / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D047630:Depsipeptides; D010456:Peptides, Cyclic; D015534:Trans-Activators; C529984:spiruchostatin B; C485419:spiruchostatin A