title: Semi-quantitative estimation of cellular SiO2 nanoparticles using flow cytometry combined with X-ray fluorescence measurements.
abstract: In this study, we have demonstrated feasibility of a semi-quantitative approach for the estimation of cellular SiO2 nanoparticles (NPs), which is based on the flow cytometry measurements of their normalized side scattering intensity. In order to improve our understanding on the quantitative aspects of cell-nanoparticle interactions, flow cytometry, transmission electron microscopy, and X-ray fluorescence experiments were carefully performed for the HeLa cells exposed to SiO2 NPs with different core diameters, hydrodynamic sizes, and surface charges. Based on the observed relationships among the experimental data, a semi-quantitative cellular SiO2 NPs estimation method from their normalized side scattering and core diameters was proposed, which can be applied for the determination of cellular SiO2 NPs within their size-dependent linear ranges.
authors: Department of Chemistry, College of Natural Sciences, Research Institute for Natural Sciences, Hanyang University, Seoul, 133-791, Republic of Korea.
authors: Seo Yeon
authors: SY
authors: Choi
authors: Nuri
authors: N
authors: Yang
authors: Soo Kyung
authors: SK
authors: Jeon
authors: Tae Hyun
authors: TH
authors: Yoon
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005434:Flow Cytometry / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000378:metabolism / Q000633:toxicity*; D012822:Silicon Dioxide / Q000378:metabolism*; D013052:Spectrometry, X-Ray Emission / Q000379:methods*
chemical_list: D012822:Silicon Dioxide
keywords: X-ray fluorescence; cellular SiO2 nanoparticles; flow cytometry; nanoparticle toxicity