title: Gene expression and metabolite changes during Tuber magnatum fruiting body storage.
abstract: The aim of this study was to investigate the impact of different 4 °C post-harvest storage periods on the quality of the white truffle Tuber magnatum. The expression of selected genes and the profiles of non-volatile metabolites have been analyzed. The up-regulation of genes related to cell wall metabolism and to a putative laccase points to cell wall modifications and browning events during cold storage. Time course RT-qPCR experiments have demonstrated that such transcription events probably depend on the ripening status, since this is delayed in partially ripe fruiting bodies. Changes in the concentrations of linoleate-derived metabolites occur during the first 3 days of considered cold storage, while the other metabolites, such as the amino acids, do not change. Taken together, the results demonstrate that complex molecular events occur in white truffles in the post-harvest period and before they are used as fresh products.
authors: Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Viale Mattioli 25, 10125, Torino, Italy.
authors: Elisa
authors: E
authors: Zampieri
authors: Flavia
authors: F
authors: Guzzo
authors: Mauro
authors: M
authors: Commisso
authors: Antonietta
authors: A
authors: Mello
authors: Paola
authors: P
authors: Bonfante
authors: Raffaella
authors: R
authors: Balestrini
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D048690:Fruiting Bodies, Fungal / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D055432:Metabolomics; D015999:Multivariate Analysis; D012331:RNA, Fungal / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D015854:Up-Regulation
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012331:RNA, Fungal