title: Label-free proteomic analysis of red blood cell membrane fractions from abdominal aortic aneurysm patients.
abstract: OBJECTIVE
  To test whether red blood cell (RBC) membrane composition is modified in abdominal aortic aneurysms (AAA) patients.
  
  
  METHODS
  RBC membrane extracts from AAA patients (aortic diameter >3 cm, n = 7) and control subjects (n = 4) were analyzed by a label-free quantitative MS-based strategy, using spectral count data. Additional validation was performed by western-blot.
  
  
  RESULTS
  Data analysis based on spectral count from MS/MS-based experiments provided us a signature of 39 proteins differentially expressed in RBC membranes between AAA and controls (changes equal/over 1.515-fold; p-values equal/lower 0.05). MS data revealed altered levels of structural membrane proteins (e.g. spectrins and ankyrin), components of the degradation machinery (proteasome subunits), and oxidative stress related proteins (e.g. catalase and peroxiredoxin-2) among others. Decreased catalase and peroxiredoxin-2 expression in RBC membrane of AAA patients compared to controls were further validated by Western blot, confirming the proteomic results.
  
  
  CONCLUSIONS
  RBCs membrane protein composition is altered in AAA patients, which could be involved in the pathological role of RBCs in aortic tissue and become potential targets to prevent AAA progression.
authors: Vascular Research Lab, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid.
authors: Roxana
authors: R
authors: Martinez-Pinna
authors: Elena
authors: E
authors: Burillo
authors: Julio
authors: J
authors: Madrigal-Matute
authors: Juan Antonio
authors: JA
authors: Lopez
authors: Emilio
authors: E
authors: Camafeita
authors: Monica Maria
authors: MM
authors: Torres-Fonseca
authors: Patricia
authors: P
authors: Llamas-Granda
authors: Jesus
authors: J
authors: Egido
authors: Jean-Baptiste
authors: JB
authors: Michel
authors: Luis Miguel
authors: LM
authors: Blanco-Colio
authors: Jose Luis
authors: JL
authors: Martin-Ventura
mesh_terms: D017544:Aortic Aneurysm, Abdominal / Q000097:blood* / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D040901:Proteomics / Q000379:methods*; D059467:Transcriptome
keywords: Abdominal aortic aneurysm; Red blood cells