title: Combination of therapy with 5-fluorouracil and cisplatin with electroporation in human ovarian carcinoma model in vitro.
abstract: High electric field, applied to plasma membrane, affects organization of the lipid molecules, generating transient hydrophilic electropores. The application of the cell membrane electroporation in combination with cytotoxic drugs could increase the drug transport into cells. This approach is known as electrochemotherapy (ECT). Our work shows new data concerning the influence of electrochemical reaction with cisplatin or with 5-fluorouracil (5-FU) on cancer ovarian cells resistant to standard therapy with cisplatin, in comparison to ECT effect on human primary fibroblasts. We investigated the effect of electroporation and electrochemotherapy with 5-FU and cisplatin on human ovarian clear-cell carcinoma cell line (OvBH-1) and epithelial ovarian carcinoma cell line (SKOV-3) - both resistant to cisplatin typically used in ovarian cancers. As control cells, human gingival fibroblasts (HGF's) from primary culture were used. Electropermeabilization efficiency was determined by FACS analysis with iodide propidium. Efficiency of electrochemotherapy was evaluated with viability assay. The cytotoxic effect was dependent on the electroporation parameters and on drug concentration. Electroporation alone only insignificantly decreased cells proliferation in OvBH-1 line; SKOV-3 line was more sensitive to the electrical field. Electrochemotherapy with cisplatin and 5-FU showed promising effects on both ovarian cell lines with recovery of normal cells revealed after 72 hours.
authors: Department of Medical Biochemistry, Wroclaw Medical University, 10, street Chalubinskiego, 50-368 Wroclaw, Poland.
authors: Jolanta
authors: J
authors: Saczko
authors: Department of Pathomorphology and Clinical Cytology, Wroclaw Medical University, 213, street Borowska, 50-556 Wroclaw, Poland.
authors: Iwona
authors: I
authors: Kamińska
authors: Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
authors: Malgorzata
authors: M
authors: Kotulska
authors: Department of Pathomorphology and Clinical Cytology, Wroclaw Medical University, 213, street Borowska, 50-556 Wroclaw, Poland.
authors: Julia
authors: J
authors: Bar
authors: Department of Medical Biochemistry, Wroclaw Medical University, 10, street Chalubinskiego, 50-368 Wroclaw, Poland.
authors: Anna
authors: A
authors: Choromańska
authors: Department of Medical Biochemistry, Wroclaw Medical University, 10, street Chalubinskiego, 50-368 Wroclaw, Poland.
authors: Nina
authors: N
authors: Rembiałkowska
authors: Department of Medical Biochemistry, Wroclaw Medical University, 10, street Chalubinskiego, 50-368 Wroclaw, Poland.
authors: Katarzyna
authors: K
authors: Bieżuńska-Kusiak
authors: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12, street Rudolf Weigl, 53-114 Wroclaw, Poland.
authors: Joanna
authors: J
authors: Rossowska
authors: Department of Dental Materials, Wroclaw Medical University, 26, street Krakowska, 50-425 Wroclaw, Poland.
authors: Danuta
authors: D
authors: Nowakowska
authors: Department of Medical Biochemistry, Wroclaw Medical University, 10, street Chalubinskiego, 50-368 Wroclaw, Poland. Electronic address: Julita.Kulbacka@umed.wroc.pl.
authors: Julita
authors: J
authors: Kulbacka
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology / Q000627:therapeutic use*; D016503:Drug Delivery Systems; D018274:Electroporation; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D005434:Flow Cytometry; D005472:Fluorouracil / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D020128:Inhibitory Concentration 50; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D002945:Cisplatin; D005472:Fluorouracil
keywords: 5-fluorouracil; Cisplatin; Electrochemotherapy; Electropermeabilization; Human fibroblasts; Ovarian carcinoma