title: Hypertrophy of ligamentum flavum in lumbar spine stenosis is associated with increased miR-155 level.
abstract: Hypertrophy of ligamentum flavum (LF) contributes to lumbar spinal stenosis (LSS) and is caused mainly by fibrosis. Recent data indicate that miR-155 plays a crucial role in the pathogenesis of different fibrotic diseases. This study aimed to test the hypothesis that miR-155 exerts effects on LF thickness by regulating collagen expression. We found that LF thickness and the expression of collagen I and, collagen III were higher in LF from LSS patients than in LF from lumbar disc herniation (LDH) patients (P < 0.01). The expression of miR-155 was significantly higher in LF from LSS group than in LF from LDH group (P < 0.01). miR-155 level was positively correlated with LF thickness (r = 0.958, P < 0.01), type I collagen level (r = 0.825, P < 0.01), and type III collagen level (r = 0.827, P < 0.01). miR-155 mimic increased mRNA and protein expression of collagen I and collagen III in fibroblasts isolated from LF, while miR-155 sponge decreased mRNA and protein expression of collagen I and III in fibroblasts. In conclusions, miR-155 is a fibrosis-associated miRNA and may play important role in the pathogenesis of LF hypertrophy.
authors: Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 1630 Dongfang Road, Shanghai 200127, China.
authors: Jianwei
authors: J
authors: Chen
authors: Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 1630 Dongfang Road, Shanghai 200127, China.
authors: Zude
authors: Z
authors: Liu
authors: Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 1630 Dongfang Road, Shanghai 200127, China.
authors: Guibin
authors: G
authors: Zhong
authors: Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 1630 Dongfang Road, Shanghai 200127, China.
authors: Lie
authors: L
authors: Qian
authors: Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 1630 Dongfang Road, Shanghai 200127, China.
authors: Zhanchun
authors: Z
authors: Li
authors: Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedics, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China.
authors: Zhiguang
authors: Z
authors: Qiao
authors: Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 1630 Dongfang Road, Shanghai 200127, China.
authors: Bin
authors: B
authors: Chen
authors: Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 1630 Dongfang Road, Shanghai 200127, China.
authors: Hantao
authors: H
authors: Wang
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism; D004549:Elastin / Q000378:metabolism; D005260:Female; D005355:Fibrosis; D015870:Gene Expression; D006801:Humans; D006984:Hypertrophy / Q000378:metabolism; D017843:Ligamentum Flavum / Q000378:metabolism / Q000473:pathology*; D008159:Lumbar Vertebrae / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D059748:Proteolysis; D013130:Spinal Stenosis / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D004549:Elastin