title: Development of a functional wound dressing composed of hyaluronic acid spongy sheet containing bioactive components: evaluation of wound healing potential in animal tests.
abstract: This study aimed to develop a novel wound dressing composed of hyaluronic acid (HA) spongy sheet containing bioactive components. The wound dressing prepared by the freeze-drying method has a two-layered structure: an upper layer composed of cross-linked high-molecular-weight HA (HMW-HA) and a lower layer composed of low-molecular-weight HA (LMW-HA) containing arginine (Arg), magnesium ascorbyl phosphate (vitamin C derivative: VC), and epidermal growth factor (EGF) (referred to as EGF-dressing). A wound dressing containing only Arg and VC was prepared in a similar manner (referred to as EGF-free-dressing). The potential of each wound dressing was evaluated in animal tests using Sprague Dawley (SD) rats and diabetic mice. In the first experiment, each wound dressing was applied to a full-thickness skin defect in the abdominal region of SD rats. Wound conditions after 1 week and 2 weeks of treatment were evaluated based on macroscopic and histological appearance. A commercially available non-woven alginate wound dressing (Alg-dressing) was used in a control group. Both EGF-free-dressing and EGF-dressing decreased wound size and promoted granulation tissue formation associated with angiogenesis more effectively when compared with Alg-dressing. In particular, EGF-dressing promoted re-epithelialization. In the second experiment, each wound dressing was applied to a full-thickness skin defect in the dorsal region of diabetic mice. Wound conditions after 1 week and 2 weeks of treatment were evaluated based on macroscopic and histological appearance. A commercially available Alg-dressing was used in a control group. Both EGF-free-dressing and EGF-dressing decreased wound size and promoted granulation tissue formation associated with angiogenesis more effectively when compared with Alg-dressing. These findings indicate that EGF-free-dressing and EGF-dressing have the potential for more effective wound healing when compared with Alg-dressing. In particular, EGF-dressing has a higher potential for wound healing when compared with EGF-free-dressing.
authors: a R&D Center for Artificial Skin , School of Allied Health Sciences, Kitasato University , 1-15-1 Kitasato, Minami-ku, Sagamihara , Kanagawa 252-0373 , Japan.
authors: Nahoko
authors: N
authors: Shimizu
authors: Daiki
authors: D
authors: Ishida
authors: Akiko
authors: A
authors: Yamamoto
authors: Misato
authors: M
authors: Kuroyanagi
authors: Yoshimitsu
authors: Y
authors: Kuroyanagi
mesh_terms: D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D051379:Mice; D051381:Rats; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D006820:Hyaluronic Acid
keywords: epidermal growth factor; epithelialization; hyaluronic acid; wound dressing; wound healing