title: In situ electrochemical assessment of cytotoxicity of chlorophenols in MCF-7 and HeLa cells.
abstract: An in situ electrochemical method was used to assess the cytotoxicity of chlorophenols using human breast cancer (MCF-7) and cervical carcinoma (HeLa) cells as models. On treatment with different chlorophenols, the electrochemical responses of the selected cells, resulting from the oxidation of guanine and xanthine in the cytoplasm, indicated the cell viability. In addition, the in situ in vitro electrochemical method was further compared with the traditional MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. Although similar cytotoxicity data were obtained from both methods, the effective concentrations of chlorophenols that inhibited 50% cell growth (EC50 values) from the electrochemical method were only slightly lower than those from the MTT assay. These results indicate that the in situ in vitro electrochemical method paves a simple, rapid, strongly responsive, and label-free way to the cytotoxicity assessment of different chlorophenol pollutants.
authors: State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, School of Environment, Northeast Normal University, Changchun 130024, China.
authors: Hongwei
authors: H
authors: Qin
authors: School of Stomatology, Jiamusi University, Jiamusi 154007, China.
authors: Jiguang
authors: J
authors: Liu
authors: State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, School of Environment, Northeast Normal University, Changchun 130024, China.
authors: Zeshi
authors: Z
authors: Zhang
authors: College of Chemistry and Pharmacy, Jiamusi University, Jiamusi 154007, China.
authors: Jinlian
authors: J
authors: Li
authors: College of Chemistry and Pharmacy, Jiamusi University, Jiamusi 154007, China.
authors: Guanggang
authors: G
authors: Gao
authors: State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, School of Environment, Northeast Normal University, Changchun 130024, China.
authors: Yuxin
authors: Y
authors: Yang
authors: State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, School of Environment, Northeast Normal University, Changchun 130024, China. Electronic address: yuanx@nenu.edu.cn.
authors: Xing
authors: X
authors: Yuan
authors: College of Chemistry and Pharmacy, Jiamusi University, Jiamusi 154007, China. Electronic address: wudmei67@163.com.
authors: Dongmei
authors: D
authors: Wu
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002733:Chlorophenols / Q000633:toxicity*; D003603:Cytotoxins / Q000633:toxicity*; D004563:Electrochemistry / Q000191:economics / Q000379:methods*; D004785:Environmental Pollutants / Q000633:toxicity*; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D012449:Safety; D013997:Time Factors; D018675:Toxicity Tests / Q000191:economics / Q000379:methods*
chemical_list: D002733:Chlorophenols; D003603:Cytotoxins; D004785:Environmental Pollutants
keywords: Chlorophenols; Cytotoxicity; Electrochemistry; In situ