title: Expression of sphingosine 1-phosphate 1-3 on penile cavernous tissue in hypertensive and normotensive rats.
abstract: OBJECTIVE
  To investigate the expression of sphingosine 1-phosphate 1-3 (S1P1-3) on penile cavernous tissues in hypertensive and normotensive rats and its association with erectile function.
  
  
  METHODS
  Ten 14-week-old male spontaneously hypertensive rats (SHRs) and 10 male age-matched normotensive Wistar-Kyoto rats were randomly designated as hypertensive rat group and normotensive control group, respectively. Mean blood pressure, serum testosterone, and maximum intracavernosal pressure/mean arterial pressure (ICPmax-to-MAP ratio) were measured. The expression of S1P1-3, endothelial nitric oxide synthase (eNOS), phosphorylated eNOS (P-eNOS), and rho-associated protein kinase 1-2 (ROCK1-2) was determined in the cavernous tissues of SHRs and Wistar-Kyoto rats by the immunohistochemical study and Western blot.
  
  
  RESULTS
  There was no significant difference in body weight and serum testosterone levels between the 2 groups. The MAP in the hypertensive rat group was significantly increased compared with the control group. After 3- and 5-V electrostimulation, the ICPmax-to-MAP ratio in the control group was significantly increased compared with the hypertensive rat group. The protein expression of eNOS, P-eNOS, and S1P1 was significantly higher in the control group than in the hypertensive rat group (P <.05). The protein expression of S1P2, S1P3, and ROCK1-2 was significantly lower in the normotensive control group than in the hypertensive rat group (P <.05).
  
  
  CONCLUSIONS
  Hypertension may impair the erectile function by means of downregulating the expression of S1P1 and upregulating the expression of S1P2-3 in cavernous tissues of SHRs.
authors: Department of Cardiovascular Diseases, Affiliated Hospital, Luzhou Medical College, Luzhou, China.
authors: Boyi
authors: B
authors: Wang
authors: Department of Vascular Surgery, Affiliated Hospital, Luzhou Medical College, Luzhou, China.
authors: Jun
authors: J
authors: Jiang
authors: Department of Cardiovascular Diseases, Affiliated Hospital, Luzhou Medical College, Luzhou, China.
authors: Zhongcai
authors: Z
authors: Fan
authors: Department of Urology, Affiliated Hospital, Luzhou Medical College, Luzhou, China. Electronic address: jiangrui@126.com.
authors: Rui
authors: R
authors: Jiang
authors: Department of Surgery, The University of Texas Medical School at Houston, Houston, TX.
authors: Run
authors: R
authors: Wang
authors: Department of Surgery, The University of Texas Medical School at Houston, Houston, TX.
authors: Haocheng
authors: H
authors: Lin
mesh_terms: D000818:Animals; D006973:Hypertension / Q000378:metabolism*; D008297:Male; D010413:Penis / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D049349:Receptors, Lysosphingolipid / Q000096:biosynthesis*
chemical_list: D049349:Receptors, Lysosphingolipid