title: Characterization of human uropathogenic ESBL-producing Escherichia coli in the Czech Republic: spread of CTX-M-27-producing strains in a university hospital.
abstract: OBJECTIVE
  The purpose of this study was to characterize the extended-spectrum β-lactamase (ESBL)-producing uropathogenic Escherichia coli (UPEC) strains isolated in the South Moravia region of the Czech Republic.
  
  
  RESULTS
  Out of 109 ESBL-producing UPEC isolates, the CTX-M-15-producing E. coli O25b-ST131 was detected in 55 (50.5%) and the CTX-M-27-producing E. coli O25b-ST131 in 40 isolates (36.7%). Most isolates were distributed among three pulsed-field gel electrophoresis clusters and were characterized by low variability relative to antibiotic resistance patterns, in E. coli phylogroups and by the prevalence of virulence and bacteriocin determinants. Despite this, 14 groups of identical isolates (comprising a total of 41 isolates) were identified when all tested parameters of E. coli were combined.
  
  
  CONCLUSIONS
  Since the occurrence of E. coli B2-O25b-ST131 CTX-M-27 was only recently described in Asia, the frequent isolation of this lineage among patients in South Moravia suggests an efficient transfer of this clone from Asian countries. The limited variability of detected parameters of ESBL-producing UPEC strains is consistent with a common origin of the analyzed isolates, in which there is an ongoing process of genetic diversification.
authors: 1 Department of Biology, Faculty of Medicine, Masaryk University , Brno, Czech Republic .
authors: Lenka
authors: L
authors: Micenková
authors: Petra
authors: P
authors: Sišková
authors: Juraj
authors: J
authors: Bosák
authors: Ivana
authors: I
authors: Jamborová
authors: Lenka
authors: L
authors: Cernohorská
authors: David
authors: D
authors: Smajs
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000453:epidemiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D006785:Hospitals, University; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D015995:Prevalence; D014774:Virulence / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C477098:CTX-M-27, E coli; D001618:beta-Lactamases