title: Association between cellular radiosensitivity and G1/G2 checkpoint proficiencies in human cholangiocarcinoma cell lines.
abstract: Cholangiocarcinoma is a destructive malignancy with a poor prognosis and lack of effective medical treatment. Radiotherapy is an alternative treatment for patients with unresectable cholangiocarcinoma. However, there are limited data on the radiation responsiveness of individual cholangiocarcinoma cells, which is a key factor that influences radiation treatment outcome. In this study, we found that cholangiocarcinoma cell lines differ remarkably in their radiosensitivity. The variation of radiosensitivity of cholangiocarcinoma cells correlates with their p53 status and existing G1 and/or G2 checkpoint defects. We also demonstrated the potential of checkpoint kinase Chk1/2 inhibition on the enhancement of the radiosensitivity of cholangiocarcinoma cells. Thus, this study provides useful information for predicting radiation response and provides evidence for the enchantment of radiotherapeutic efficiency by targeting checkpoint kinase Chk1/2 in some subpopulations of cholangiocarcinoma patients.
authors: Radiobiology Research Laboratory, Department of Radiation Technology, Faculty of Allied Health Science, Naresuan University, Phitsanulok 65000, Thailand.
authors: Arunee
authors: A
authors: Hematulin
authors: Independent Researcher, D-93051 Regensburg, Germany.
authors: Daniel
authors: D
authors: Sagan
authors: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Kanlayanee
authors: K
authors: Sawanyawisuth
authors: Department of Forensic Science, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Wunchana
authors: W
authors: Seubwai
authors: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Sopit
authors: S
authors: Wongkham
mesh_terms: D001650:Bile Duct Neoplasms / Q000532:radiotherapy*; D001653:Bile Ducts, Intrahepatic / Q000528:radiation effects; D045744:Cell Line, Tumor; D018281:Cholangiocarcinoma / Q000532:radiotherapy*; D004307:Dose-Response Relationship, Radiation; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D011836:Radiation Tolerance / Q000187:drug effects; D013876:Thiophenes / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014508:Urea / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: C532363:3-(carbamoylamino)-5-(3-fluorophenyl)-N-(3-piperidyl)thiophene-2-carboxamide; C495901:TP53 protein, human; D013876:Thiophenes; D016159:Tumor Suppressor Protein p53; D014508:Urea