title: Simultaneous determination of propofol and remifentanil in rat plasma by liquid chromatography-tandem mass spectrometry: application to preclinical pharmacokinetic drug-drug interaction analysis.
abstract: Propofol (Pro) is an ultra-short-acting hypnotic agent used for general anesthesia that has no analgesic properties. Remifentanil (Rem) is an ultra-short-acting opioid administered concomitantly as an analgesic with Pro. To evaluate the pharmacokinetic interactions between Pro and Rem, we developed and validated a method combining high-performance liquid chromatography with tandem mass spectrometry for simultaneous determination of Pro and Rem. The proposed method was successfully used to study the pharmacokinetic interactions of Pro and Rem coadministered to rats.
authors: Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8131, Japan.
authors: Mohamed A
authors: MA
authors: El Hamd
authors: Mitsuhiro
authors: M
authors: Wada
authors: Rie
authors: R
authors: Ikeda
authors: Shigeru
authors: S
authors: Kawakami
authors: Naotaka
authors: N
authors: Kuroda
authors: Kenichiro
authors: K
authors: Nakashima
mesh_terms: D000701:Analgesics, Opioid / Q000097:blood / Q000493:pharmacokinetics; D018686:Anesthetics, Intravenous / Q000097:blood / Q000493:pharmacokinetics; D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid / Q000379:methods; D004347:Drug Interactions; D006993:Hypnotics and Sedatives / Q000097:blood / Q000493:pharmacokinetics; D057230:Limit of Detection; D008297:Male; D010880:Piperidines / Q000097:blood* / Q000493:pharmacokinetics; D015742:Propofol / Q000097:blood* / Q000493:pharmacokinetics; D017208:Rats, Wistar; D000077208:Remifentanil; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000701:Analgesics, Opioid; D018686:Anesthetics, Intravenous; D006993:Hypnotics and Sedatives; D010880:Piperidines; D000077208:Remifentanil; D015742:Propofol
keywords: LC-MS/MS; pharmacokinetic interactions; propofol; rat plasma; remifentanil