title: Chemoselective reduction and self-immolation based FRET probes for detecting hydrogen sulfide in solution and in cells.
abstract: Hydrogen sulfide (H2S) has been regarded as the third gaseous transmitter. Based on the mechanism of chemoselective azido reduction and self-immolation, five fluorescence resonance energy transfer (FRET) probes for the detection of H2S were designed and synthesized. The effect of functional substitution of the self-immolative moiety on azido reduction and quinone-methide rearrangement were investigated. Their fluorescence responses and chemoselectivity for H2S detection were evaluated in solutions and in cells. This strategy may provide a general route for designing H2S probes with many commercially available FRET pairs.
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China.
authors: Bifeng
authors: B
authors: Chen
authors: Peng
authors: P
authors: Wang
authors: Qingqing
authors: Q
authors: Jin
authors: Xinjing
authors: X
authors: Tang
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D012996:Solutions
chemical_list: D005456:Fluorescent Dyes; D012996:Solutions; D006862:Hydrogen Sulfide