title: In vitro expression of Candida albicans alcohol dehydrogenase genes involved in acetaldehyde metabolism.
abstract: Alcohol consumption is a risk factor for oral cancer, possibly via its conversion to acetaldehyde, a known carcinogen. The oral commensal yeast Candida albicans may be one of the agents responsible for this conversion intra-orally. The alcohol dehydrogenase (Adh) family of enzymes are involved in acetaldehyde metabolism in yeast but, for C. albicans it is not known which family member is responsible for the conversion of ethanol to acetaldehyde. In this study we determined the expression of mRNAs from three C. albicans Adh genes (CaADH1, CaADH2 and CaCDH3) for cells grown in different culture media at different growth phases by Northern blot analysis and quantitative reverse transcription polymerase chain reaction. CaADH1 was constitutively expressed under all growth conditions but there was differential expression of CaADH2. CaADH3 expression was not detected. To investigate whether CaAdh1p or CaAdh2p can contribute to alcohol catabolism in C. albicans, each gene from the reference strain C. albicans SC5314 was expressed in Saccharomyces cerevisiae. Cell extracts from an CaAdh1p-expressing S. cerevisiae recombinant, but not an CaAdh2p-expressing recombinant, or an empty vector control strain, possessed ethanol-utilizing Adh activity above endogenous S. cerevisiae activity. Furthermore, expression of C. albicans Adh1p in a recombinant S. cerevisiae strain in which the endogenous ScADH2 gene (known to convert ethanol to acetaldehyde in this yeast) had been deleted, conferred an NAD-dependent ethanol-utilizing, and so acetaldehyde-producing, Adh activity. We conclude that CaAdh1p is the enzyme responsible for ethanol use under in vitro growth conditions, and may contribute to the intra-oral production of acetaldehyde.
authors: Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors: M M
authors: MM
authors: Bakri
authors: A M
authors: AM
authors: Rich
authors: R D
authors: RD
authors: Cannon
authors: A R
authors: AR
authors: Holmes
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism*; D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism*; D015152:Blotting, Northern; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D019295:Computational Biology; D003470:Culture Media; D000431:Ethanol / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D000438:Alcohols; D003470:Culture Media; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000079:Acetaldehyde
keywords: Candida albicans; acetaldehyde; alcohol dehydrogenase