title: Intracellular glycerol accumulation in light-limited Dunaliella tertiolecta culture is determined by partitioning of glycerol across the cell membrane.
abstract: Dunaliella accumulates intracellular glycerol to counterbalance the extracellular salinity. In N-limited chemostat cultures of D. tertiolecta, total glycerol production (sum of intracellular and extracellular) and intracellular glycerol content were proportional to the salinity of the culture medium. In the light-limited D. tertiolecta culture, total glycerol output (sum of intracellular and extracellular) was relatively constant at different salinities (0.5 and 2.0 M), while the intracellular glycerol content was proportional to the culture medium salinity, that is, the cells released less glycerol into the culture medium, rather than de novo synthesis of glycerol at high culture medium salinity. The study implies different regulatory mechanisms in the accumulation of intracellular glycerol in N-limited and light-limited D. tertiolecta in response to salinity.
authors: Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors: Daphne H P
authors: DH
authors: Ng
authors: Chin Seng
authors: CS
authors: Low
authors: Yvonne Y S
authors: YY
authors: Chow
authors: Yuan Kun
authors: YK
authors: Lee
mesh_terms: D001692:Biological Transport / Q000528:radiation effects*; D002462:Cell Membrane / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005990:Glycerol / Q000378:metabolism*; D008027:Light; D009584:Nitrogen / Q000378:metabolism; D009997:Osmotic Pressure; D054712:Salinity; D013312:Stress, Physiological; D016823:Volvocida / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects*
chemical_list: D003470:Culture Media; D009584:Nitrogen; D005990:Glycerol
keywords: bioremediation; microalgae; osmoregulation; photosynthesis