title: Silencing NPAS2 promotes cell growth and invasion in DLD-1 cells and correlated with poor prognosis of colorectal cancer.
abstract: Emerging evidences show that circadian rhythm disorder is an important factor of tumor initiation and development. Neuronal PAS domain protein2 (NPAS2), which is the largest circadian gene, has been proved to be a novel prognostic biomarker in breast cancer and non-Hodgkin's lymphoma. However, the potential functions of NPAS2 in colorectal cancer are still unknown. In our present study, we detected the mRNA expressions of NPAS2 in 108 CRC patients by RT-PCR, and found that NPAS2 expression was significantly down-regulated in tumor tissues than that in NATs. Clinicopathologic analysis revealed that low expression of NPAS2 was associated with the tumor size, TNM stage and tumor distance metastasis in colorectal cancer (p<0.05). Furthermore, we effectively down-regulated NPAS2 mRNA expression by transfecting RNA interfere fragments into DLD-1 cells, and our results in vitro demonstrated that silencing NPAS2 expression could promote cell proliferation, cell invasion and increase the wound healing ability (p<0.05). However, down-regulating NPAS2 expression did not influence the apoptotic rate in DLD-1 cells (p>0.05). In conclusion, our study suggested that NPAS2, functioned as a potential tumor suppressor gene, could serve as a promising target and potential prognostic indicator for colorectal cancer.
authors: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Xiaofeng
authors: X
authors: Xue
authors: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Fei
authors: F
authors: Liu
authors: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Ye
authors: Y
authors: Han
authors: Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Department of Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China.
authors: Pu
authors: P
authors: Li
authors: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Bin
authors: B
authors: Yuan
authors: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Xu
authors: X
authors: Wang
authors: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Yan
authors: Y
authors: Chen
authors: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Yuting
authors: Y
authors: Kuang
authors: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. Electronic address: strexboy@163.com.
authors: Qiaoming
authors: Q
authors: Zhi
authors: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. Electronic address: zhaohong600@sina.com.
authors: Hong
authors: H
authors: Zhao
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D020868:Gene Silencing; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C104264:NPAS2 protein, human
keywords: Circadian gene; Colorectal cancer; NPAS2