title: Chemiluminescence and Toluidine Blue as Diagnostic Tools for Detecting Early Stages of Oral Cancer: An invivo Study.
abstract: BACKGROUND
  There is a need for development and use of diagnostic aids that help the dental specialist more readily identify and assess Potentially Malignant Epithelial Lesions (PMELs) and Oral Squamous Cell Carcinoma (OSCC). This study was done to assess the value of two such commercially available tools: chemiluminescent light kit or ViziLite and 1% toluidine blue.
  
  
  OBJECTIVE
  a) To detect epithelial dysplastic changes using chemiluminescene (commercially available as ViziLite) and toluidine blue staining in PMELs and OSCC patients and compare the results obtained with histopathological examination. b) To determine whether these techniques can be used to detect early epithelial dysplastic changes in clinically normal appearing oral mucosa of high risk (with habits) patients.
  
  
  METHODS
  A total of 60 patients- 25 patients with PMELs, specifically oral leukoplakia, 10 patients with clinically diagnosed OSCC and 25 high risk patients with no clinically visible lesion, were screened with ViziLite and toluidine blue staining; followed by incisional biopsy.
  
  
  RESULTS
  Sensitivity and specificity of ViziLite were calculated to be 95.45% and 84.6% respectively. ViziLite detected early epithelial dysplastic changes in one high risk patient with clinically normal appearing oral mucosa. Sensitivity and specificity of toluidine blue were calculated to be 86.36% and 76.9% respectively.
  
  
  CONCLUSIONS
  ViziLite was relatively reliable in screening PMELs compared to toluidine blue, and was a useful chair side diagnostic aid.
authors: Senior Lecturer, Department of Oral Medicine and Radiology, Institute of Dental Sciences , Sehora, Jammu, India .
authors: Neha
authors: N
authors: Vashisht
authors: Professor and Head, Department of Oral Medicine and Radiology, Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India .
authors: A
authors: A
authors: Ravikiran
authors: Reader, Department of Oral Medicine and Radiology, Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India .
authors: Y
authors: Y
authors: Samatha
authors: Senior Lecturer, Department of Oral Medicine and Radiology, Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India .
authors: Purna Chandra
authors: PC
authors: Rao
authors: Senior Lecturer, Department of Oral Medicine and Radiology, Mamata Dental College , Khamam, Andhra Pradesh, India .
authors: Ravindra
authors: R
authors: Naik
authors: Assistant Professor, Department of Prosthodontics, Government Dental College , Shimla, India .
authors: Divy
authors: D
authors: Vashisht
keywords: Chemiluminescence; OSCC; PMELs; Toluidine blue; ViziLite