title: Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken: relevance of in vitro and ex vivo methods for strains characterization.
abstract: Based on the natural benefits of the indigenous microbiota, lactic acid bacteria (LAB) from poultry origin were isolated from hens and broilers intestine, and their probiotic potential was further studied. The tolerance to digestion, adhesion, capture of a mannose-binding lectin, absence of virulent factors and antibiotic resistances were studied. Different in vitro and ex vivo assays were performed to select tolerant and adherent strains because standardized protocols have not been defined. Fourteen strains highly tolerant to gastrointestinal digestion were genetically identified. Hydrophobic surfaces were not required for the bacterial adhesion and only nine strains adhered ex vivo to the intestinal mucosa. Three strains captured a lectin of the same specificity of Type-1 fimbriae. Virulence factors were absent but some strains evidenced multiple antibiotic resistances. These results provide bases for a future standardization of methods for the selection of probiotic strains intended to reinforce the microbiota of newly hatched chickens.
authors: Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, T4000ILC San Miguel de Tucumán, Argentina.
authors: Jaime D
authors: JD
authors: Babot
authors: Universidad Nacional de Tucumán, Ayacucho 471, T4000ILC San Miguel de Tucumán, Argentina.
authors: Eloy
authors: E
authors: Argañaraz-Martínez
authors: Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, T4000ILC San Miguel de Tucumán, Argentina.
authors: Lucila
authors: L
authors: Saavedra
authors: Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, T4000ILC San Miguel de Tucumán, Argentina; Universidad Nacional de Tucumán, Ayacucho 471, T4000ILC San Miguel de Tucumán, Argentina.
authors: María C
authors: MC
authors: Apella
authors: Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, T4000ILC San Miguel de Tucumán, Argentina; Universidad Nacional de Tucumán, Ayacucho 471, T4000ILC San Miguel de Tucumán, Argentina. Electronic address: apchaia@fbqf.unt.edu.ar.
authors: Adriana
authors: A
authors: Perez Chaia
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000382:microbiology*; D001422:Bacterial Adhesion / Q000235:genetics / Q000502:physiology; D001424:Bacterial Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary; D002645:Chickens / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics / Q000502:physiology; D005260:Female; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology; D066298:In Vitro Techniques; D007422:Intestines / Q000382:microbiology*; D007777:Lactobacillaceae / Q000235:genetics* / Q000502:physiology*; D064307:Microbiota / Q000235:genetics / Q000502:physiology; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control; D019936:Probiotics / Q000627:therapeutic use*; D012641:Selection, Genetic / Q000235:genetics*; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology
chemical_list: D037521:Virulence Factors
keywords: Lactic acid bacteria; Poultry; Probiotic; Safety; Selection protocols