title: Keratinocyte growth factor-2 is protective in lipopolysaccharide-induced acute lung injury in rats.
abstract: Keratinocyte growth factor-2 (KGF-2) plays a key role in lung development, but its role in acute lung injury has not been well characterized. Lipopolysaccharide instillation caused acute lung injury, which significantly elevated lung wet-to-dry weight ratio, protein and neutrophils in bronchoalveolar lavage fluid (BALF), inhibited surfactant protein A and C expression in lung tissue, and increased pathological injury. Pretreatment with KGF-2 improved the above lung injury parameters, partially restored surfactant protein A and C expression, and KGF-2 given 2-3 days before LPS challenge showed maximum lung injury improvement. Pretreatment with KGF-2 also markedly reduced the levels of TNF-α, MIP-2, IL-1β and IL-6 in BALF and the levels of IL-1β and IL-6 in lung tissue. Histological analysis showed there was increased proliferation of alveolar type II epithelial cells in lung parenchyma, which reached maximal 2 days after KGF-2 instillation. Intratracheal administration of KGF-2 attenuates lung injury induced by LPS, suggesting KGF-2 may be potent in the intervention of acute lung injury.
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
authors: Lin
authors: L
authors: Tong
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
authors: Jing
authors: J
authors: Bi
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
authors: Xiaodan
authors: X
authors: Zhu
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
authors: Guifang
authors: G
authors: Wang
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
authors: Jie
authors: J
authors: Liu
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
authors: Linyi
authors: L
authors: Rong
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
authors: Qin
authors: Q
authors: Wang
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
authors: Nuo
authors: N
authors: Xu
authors: Division of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
authors: Ming
authors: M
authors: Zhong
authors: Division of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
authors: Duming
authors: D
authors: Zhu
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China; Shanghai Public Health Clinical Center, Shanghai 201508, PR China; Zhongshan Hospital, Qingpu Branch, Fudan University, Shanghai 201700, PR China. Electronic address: ylsong70@gmail.com.
authors: Yuanlin
authors: Y
authors: Song
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China. Electronic address: bai.chunxue@zs-hospital.sh.cn.
authors: Chunxue
authors: C
authors: Bai
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D051526:Fibroblast Growth Factor 10 / Q000627:therapeutic use*; D019394:Ki-67 Antigen / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008168:Lung / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D009504:Neutrophils / Q000473:pathology; D010455:Peptides / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D016207:Cytokines; D051526:Fibroblast Growth Factor 10; D019394:Ki-67 Antigen; D008070:Lipopolysaccharides; D010455:Peptides; D012333:RNA, Messenger; C077063:Sftpc protein, rat
keywords: Acute lung injury; Epithelial cells; Keratinocyte growth factor-2; Lipopolysaccharide; Pulmonary surfactant proteins