title: EF1A interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication.
abstract: Transmissible gastroenteritis coronavirus (TGEV) is an enteropathogenic coronavirus that causes diarrhea in pigs, which is correlated with high morbidity and mortality in suckling piglets. Using the method of GST pull-down with the nucleocapsid (N), N protein was found to interact with swine testes (ST) cells elongation factor 1-alpha (EF1A), an essential component of the translational machinery with an important role in cells. In vitro and in virus-infected cells interaction was then confirmed by co-precipitation. Knockdown of EF1A impairs N protein proliferation and TGEV replication in host cell. It was demonstrated that EF1A plays a role in TGEV replication. The present study thus provides a protein-related information that should be useful for underlying mechanism of coronavirus replication.
authors: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors: Xin
authors: X
authors: Zhang
authors: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors: Hongyan
authors: H
authors: Shi
authors: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors: Jianfei
authors: J
authors: Chen
authors: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors: Da
authors: D
authors: Shi
authors: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors: Changlong
authors: C
authors: Li
authors: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: fengli_h@163.com.
authors: Li
authors: L
authors: Feng
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017934:Coronavirus / Q000378:metabolism*; D005761:Gastroenteritis, Transmissible, of Swine / Q000821:virology*; D005786:Gene Expression Regulation; D008297:Male; D019590:Nucleocapsid Proteins / Q000378:metabolism*; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D013737:Testis / Q000166:cytology / Q000378:metabolism; D005760:Transmissible gastroenteritis virus; D014779:Virus Replication / Q000502:physiology*
chemical_list: D019590:Nucleocapsid Proteins; D020648:Peptide Elongation Factor 1
keywords: EF1A; Nucleocapsid; Transmissible gastroenteritis coronavirus