title: Pearl shaped highly sensitive Mn3O4 nanocomposite interface for biosensor applications.
abstract: An electrochemical biosensor based on manganese oxide (Mn3O4) and chitosan (Cn) nanocomposite has been fabricated for fish freshness detection. The electrophoretic deposition of Mn3O4 nanoparticles (15-20 nm) with Cn has changed their morphological arrangement leading to pearl shaped of Mn3O4-Cn nanocomposite on indium tin oxide substrate. Size and morphology of nanocomposite have been confirmed by high resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results of electrochemical response reveal that this improved sensor has widest detection range of xanthine concentration from 1 to 500 µM and excellent sensitivity of 1.46 μA µM(-1) cm(-2). The fabricated XOx/Mn3O4-Cn/ITO biosensor can detect as low as 1.31 μM of xanthine and lower Km value of 0.018 μM confirming its superior affinity towards the nanocomposite film.
authors: Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India. Electronic address: kamilreza@gmail.com.
authors: K
authors: K
authors: Kamil Reza
authors: Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India.
authors: Nawab
authors: N
authors: Singh
authors: Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India.
authors: Surendra K
authors: SK
authors: Yadav
authors: Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India.
authors: Manish Kumar
authors: MK
authors: Singh
authors: Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India.
authors: A M
authors: AM
authors: Biradar
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D048271:Chitosan / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation; D004566:Electrodes; D004867:Equipment Design; D057230:Limit of Detection; D017895:Manganese Compounds / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry*; D014961:X-Ray Diffraction; D019820:Xanthine / Q000032:analysis*
chemical_list: D017895:Manganese Compounds; D010087:Oxides; D019820:Xanthine; C027424:manganese oxide; D048271:Chitosan
keywords: Electrochemical sensor; Fish freshness; Mn(3)O(4) nanocomposite; Xanthine oxidase