title: Evaluation of VITEK 2 for discriminating Trichosporon species: misidentification of Trichosporon non-T. asahii.
abstract: The VITEK 2 system was evaluated for the identification of 74 Trichosporon invasive and non-invasive clinical isolates, comparing its results with the IGS1 sequencing. The system correctly identified Trichosporon asahii but not non-T. asahii isolates, which represented nearly 50% of the invasive infections in our nosocomial setting.
authors: Laboratory of Medical Mycology (LIM-53) - Clinical Dermatology Division, Hospital das Clínicas da FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Brazil.
authors: Dulce Sachiko Yamamoto
authors: DS
authors: de Figueiredo
authors: Microbiology Section, Central Laboratory Division (LIM-03), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors: João Nobrega
authors: JN
authors: de Almeida
authors: Microbiology Section, Central Laboratory Division (LIM-03), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors: Adriana Lopes
authors: AL
authors: Motta
authors: Mycology Unit, Adolfo Lutz Institute, Public Health Reference Laboratory, Secretary of Health, São Paulo State, Brazil.
authors: Dulcilena Mattos
authors: DM
authors: Castro e Silva
authors: Mycology Unit, Adolfo Lutz Institute, Public Health Reference Laboratory, Secretary of Health, São Paulo State, Brazil.
authors: Maria Walderez
authors: MW
authors: Szeszs
authors: Laboratory of Medical Mycology (LIM-53) - Clinical Dermatology Division, Hospital das Clínicas da FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Brazil. Electronic address: gildamdn@usp.br.
authors: Gilda Maria Barbaro
authors: GM
authors: Del Negro
mesh_terms: D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D005838:Genotype; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods* / Q000592:standards*; D010802:Phylogeny; D015203:Reproducibility of Results; D014250:Trichosporon / Q000145:classification* / Q000235:genetics*; D060586:Trichosporonosis / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal
keywords: Misidentification; Sequencing; Trichosporon; VITEK 2