title: Anti-hypernociceptive effect of mangiferin in persistent and neuropathic pain models in rats.
abstract: The present study examines the possible effect of the glucosylxanthone mangiferin (MG) on pain-related behaviors in a tonic acute pain model (formalin test at 5%) and in a chronic constriction injury (CCI) model to clarify the underlying transient and long-term mechanisms. Acute administration of MG (10-100mg/kg, i.p.) reduced licking/biting exclusivity in the tonic phase of formalin test in a naloxone and yohimbine-sensitive manner. This effect was enhanced by a nonselective nitric oxide synthase (NOS) inhibitor (NG-monomethyl-L-arginine) and by a non-competitive N-methyl-D-aspartate (NMDA) antagonist (ketamine), but it was reversed by the NOS substrate (L-arginine). Pre-treatment with intrathecal yohimbine prevented the anti-hypernociceptive effect of systemic MG. Pre-treatment during 4 days before surgical and 3 days after CCI with MG (50mg/kg, i.p.) reduced mechanical hypernociception and decreased the signs of Wallerian degeneration (WD) of the sciatic nerve. MG improved the PC-12 cellular viability exposure to glutamate-mediated neuronal death, also involved in neuropathic pain. The findings of this study suggest that MG shows ability to decrease tonic pain in the formalin test. A transient activity of this xanthone on nociceptive pathways mediated by α2 adrenergic receptors in cooperation with the opioid system could be involved, at least in part, in this effect. Its neuroprotective effect by preventing WD in mononeuropathic rats could be implicated in the mechano-antihypernociceptive long term mechanisms.
authors: Laboratorio de Neurofarmacología, Centro de Investigación y Desarrollo de Medicamentos, Ave. 26 No. 1605, Nuevo Vedado, La Habana 10600, Cuba.
authors: Bárbara B
authors: BB
authors: Garrido-Suárez
authors: Departamento de Ciencias Farmacéuticas, Facultad de Ciencias, Edificio Ñ3, Universidad Católica del Norte, Angamos 0610, Antofagasta 1270709, Chile. Electronic address: gabinocl@yahoo.com.
authors: Gabino
authors: G
authors: Garrido
authors: Laboratorio de Neurofarmacología, Centro de Investigación y Desarrollo de Medicamentos, Ave. 26 No. 1605, Nuevo Vedado, La Habana 10600, Cuba.
authors: Marian
authors: M
authors: Castro-Labrada
authors: Laboratorio de Neurofarmacología, Centro de Investigación y Desarrollo de Medicamentos, Ave. 26 No. 1605, Nuevo Vedado, La Habana 10600, Cuba.
authors: Nelson
authors: N
authors: Merino
authors: Laboratorio de Neurofarmacología, Centro de Investigación y Desarrollo de Medicamentos, Ave. 26 No. 1605, Nuevo Vedado, La Habana 10600, Cuba.
authors: Odalys
authors: O
authors: Valdés
authors: Centro de Bioproductos Marinos, Loma y 37, Nuevo Vedado, La Habana 10300, Cuba.
authors: Idania
authors: I
authors: Rodeiro
authors: Centro de Bioproductos Marinos, Loma y 37, Nuevo Vedado, La Habana 10300, Cuba.
authors: Ivonnes
authors: I
authors: Hernández
authors: Department of Pharmacology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirão Preto, SP, Brazil.
authors: Jozi
authors: J
authors: Godoy-Figueiredo
authors: Department of Pharmacology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirão Preto, SP, Brazil.
authors: Sergio H
authors: SH
authors: Ferreira
authors: Laboratorio de Neurofarmacología, Centro de Investigación y Desarrollo de Medicamentos, Ave. 26 No. 1605, Nuevo Vedado, La Habana 10600, Cuba.
authors: René
authors: R
authors: Delgado-Hernández
mesh_terms: D000700:Analgesics / Q000627:therapeutic use*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004195:Disease Models, Animal; D008297:Male; D009437:Neuralgia / Q000188:drug therapy*; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D044004:Xanthones / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000700:Analgesics; D044004:Xanthones; C013592:mangiferin
keywords: Chronic constriction injury; Formalin test; Hyperalgesia; Mangiferin; Neuropathic pain