title: Packaging guest proteins into the encapsulin nanocompartment from Rhodococcus erythropolis N771.
abstract: The encapsulin nanocompartment from Rhodococcus erythropolis N771 (Reencapsulin) was expressed and purified in wild-type and C-terminally His-tagged forms. Negative-stained transmission electron microscopy, field-flow fractionation combined with multi-angle light scattering and dynamic light scattering analyses showed that 60 Reencapsulin monomers were assembled as a spherical particle with a diameter of 28 nm. Heterogeneous guest proteins such as EGFP and firefly luciferase were packaged into the internal cavity of the Reencapsulin nanocompartment by fusing the C-terminal 37-amino-acid sequence of the R. erythropolis N771 DypB peroxidase to the C-terminus. Reencapsulin has the potential to package target proteins in its internal cavity and/or display them on its external surface, making it a feasible carrier for nanotechnology applications.
authors: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors: Akio
authors: A
authors: Tamura
authors: Yosuke
authors: Y
authors: Fukutani
authors: Taku
authors: T
authors: Takami
authors: Motoko
authors: M
authors: Fujii
authors: Yuki
authors: Y
authors: Nakaguchi
authors: Yoshihiko
authors: Y
authors: Murakami
authors: Keiichi
authors: K
authors: Noguchi
authors: Masafumi
authors: M
authors: Yohda
authors: Masafumi
authors: M
authors: Odaka
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010544:Peroxidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055550:Protein Stability; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012240:Rhodococcus / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D010544:Peroxidases
keywords: DypB peroxidase; bacterial nanocompartment; encapsulin; nanobiotechnology; protein nanoparticle