title: Tissue extracellular matrix nanoparticle presentation in electrospun nanofibers.
abstract: Biomaterials derived from the decellularization of mature tissues retain biological and architectural features that profoundly influence cellular activity. However, the clinical utility of such materials remains limited as the shape and physical properties are difficult to control. In contrast, scaffolds based on synthetic polymers can be engineered to exhibit specific physical properties, yet often suffer from limited biological functionality. This study characterizes composite materials that present decellularized extracellular matrix (DECM) particles in combination with synthetic nanofibers and examines the ability of these materials to influence stem cell differentiation. Mechanical processing of decellularized tissues yielded particles with diameters ranging from 71 to 334 nm. Nanofiber scaffolds containing up to 10% DECM particles (wt/wt) derived from six different tissues were engineered and evaluated to confirm DECM particle incorporation and to measure bioactivity. Scaffolds containing bone, cartilage, and fat promoted osteogenesis at 1 and 3 weeks compared to controls. In contrast, spleen and lung DECM significantly reduced osteogenic outcomes compared to controls. These findings highlight the potential to incorporate appropriate source DECM nanoparticles within nanofiber composites to design a scaffold with bioactivity targeted to specific applications.
authors: Translational Tissue Engineering Center and Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD 21287, USA ; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21287, USA.
authors: Matt
authors: M
authors: Gibson
authors: Translational Tissue Engineering Center and Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD 21287, USA ; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21287, USA.
authors: Vince
authors: 0000-0002-8274-1173
authors: V
authors: Beachley
authors: Translational Tissue Engineering Center and Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD 21287, USA ; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21287, USA.
authors: Jeannine
authors: 0000-0001-6354-5436
authors: J
authors: Coburn
authors: Translational Tissue Engineering Center and Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD 21287, USA ; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21287, USA.
authors: Pierre Alain
authors: 0000-0002-2808-6112
authors: PA
authors: Bandinelli
authors: Translational Tissue Engineering Center and Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD 21287, USA ; Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21287, USA.
authors: Hai-Quan
authors: HQ
authors: Mao
authors: Translational Tissue Engineering Center and Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD 21287, USA ; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21287, USA.
authors: Jennifer
authors: J
authors: Elisseeff
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D015415:Biomarkers / Q000378:metabolism; D002450:Cell Communication; D048430:Cell Shape; D002470:Cell Survival; D005109:Extracellular Matrix / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014867:Water / Q000737:chemistry
chemical_list: D015415:Biomarkers; D014867:Water