title: The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.).
abstract: We present the first report of a virus infecting the subtropical tree crop yerba mate (Ilex paraguariensis St. Hil.). Total RNA purification, followed by next-generation sequencing, transcripts assembly and annotation, resulted in the identification of a new endornavirus species infecting yerba mate. The complete sequence of the linear dsRNA viral genome is 13,954-nt long, contains a single 13,743 nt ORF, and presents a 149 nt 5'UTR and a 61 nt 3'UTR. The predicted ORF encodes a 4,581 aa polypeptide with a UDP-glucose glycosyl-transferase, a capsular polysaccharide synthesis protein, and a RNA-dependent RNA polymerase domain. The name yerba mate endornavirus is proposed for the identified virus. Due to the intriguing peculiarities of this virus family, and the complete lack of the yerba mate virus literature, we consider that the information reported here will be helpful in leading to a new and needed attention to this important topic and crop.
authors: Instituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), X5020ICA, Córdoba, Argentina, hdebat@correo.inta.gov.ar.
authors: Humberto J
authors: HJ
authors: Debat
authors: Mauro
authors: M
authors: Grabiele
authors: Patricia M
authors: PM
authors: Aguilera
authors: Rosana
authors: R
authors: Bubillo
authors: Pedro D
authors: PD
authors: Zapata
authors: Dardo A
authors: DA
authors: Marti
authors: Daniel A
authors: DA
authors: Ducasse
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D016000:Cluster Analysis; D016679:Genome, Viral; D030019:Ilex paraguariensis / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010942:Plant Viruses / Q000235:genetics* / Q000302:isolation & purification; D020815:Polyproteins / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D020815:Polyproteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral