title: Self-assembled DNA nanostructures prepared by rolling circle amplification for the delivery of siRNA conjugates.
abstract: Inspired by the isothermal enzymatic process of rolling circle amplification (RCA) of DNA strands, we have developed a system to achieve more than a 200-fold increase in the synthesis of DNA nanostructures using a single-stranded circular DNA template. The amplified DNA nanostructures have shown efficient delivery of folic acid (FA) conjugated siRNAs into KB cells with a dose dependent gene silencing.
authors: College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea. hyukjin@ewha.ac.kr.
authors: Cheol Am
authors: CA
authors: Hong
authors: Bora
authors: B
authors: Jang
authors: Eun Hye
authors: EH
authors: Jeong
authors: Hansaem
authors: H
authors: Jeong
authors: Hyukjin
authors: H
authors: Lee
mesh_terms: D002460:Cell Line; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005492:Folic Acid / Q000737:chemistry; D006801:Humans; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D004247:DNA; D005492:Folic Acid