title: Interaction of hydrophobic components in female urine before and after childbirth with P-glycoprotein in vitro.
abstract: The first urine in the morning (total 15 samples) and whole day urine (total 4 days, 17 samples) were collected from a young healthy woman during the pregnancy and lactation period, to examine the possible interactions of urine components (methanol extracts) with P-glycoprotein (P-gp) and multidrug resistance-associated proteins (MRPs). The interaction was evaluated by measuring the intracellular accumulation of rhodamine123, a P-gp substrate, in LLC-GA5-COL150 cells, or calcein, an MRP substrate, in Caco-2 cells in the absence and presence of urine components. Four first urine samples out of 12 collected before childbirth and one sample out of three collected after childbirth suppressed P-gp function significantly. The effect of pregnancy and lactation on P-gp inhibitory potencies of urine components was not observed. The whole day urine samples showed a clear circadian rhythm, in which three first urine samples in the morning out of four showed greater P-gp inhibitory potencies than other daytime samples. Interaction of urine components with MRPs was not detected. In conclusion, the concentration of endogenous P-gp inhibitor(s) was higher in the first urine in the morning, showing a clear circadian rhythm. Normal pregnancy and lactation appeared not to significantly affect the P-gp inhibitory potencies of urine components.
authors: T
authors: T
authors: Yokooji
authors: Y
authors: Y
authors: Kameda
authors: M
authors: M
authors: Utsumi
authors: N
authors: N
authors: Mori
authors: T
authors: T
authors: Murakami
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics / Q000378:metabolism; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000652:urine*; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis; D000328:Adult; D015153:Blotting, Western; D018938:Caco-2 Cells; D002460:Cell Line; D002940:Circadian Rhythm; D005260:Female; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes; D006801:Humans; D007774:Lactation / Q000502:physiology; D049590:Postpartum Period / Q000652:urine*; D011247:Pregnancy; D020112:Rhodamine 123; D055815:Young Adult
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D005452:Fluoresceins; D005456:Fluorescent Dyes; D020112:Rhodamine 123; C007740:fluorexon