title: DNA Vaccination Techniques.
abstract: Multiple sclerosis (MS) is the most common inflammatory, demyelinating, and neurodegenerative disorder of the central nervous system (CNS) in humans. Although the etiology of MS remains unknown, several lines of evidence support the notion that autoimmunity against components of the myelin sheath plays a major role in susceptibility to and development of the disease. At present, there are no approved MS therapies aimed specifically toward downregulating antigen-specific autoreactive immune cells. One antigen-specific approach that appears promising for the treatment of MS is DNA vaccination. This technique has demonstrated efficacy in clinical trials while maintaining safety.Here, we describe the generation of DNA vaccines containing immunologically relevant antigens of MS. Moreover, we present a detailed protocol for the prophylactic and therapeutic administration of DNA vaccines via intramuscular injection targeting on the development of experimental autoimmune encephalomyelitis (EAE), an animal model resembling MS.
authors: Department of Neurology-Neuroimmunology, Centre d'Esclerosi Múltiple de Catalunya, Cemcat, Institut de Recerca Vall d'Hebron, Hospital Universitari Vall d´Hebron (HUVH), Barcelona, Spain, nicolas.fissolo@vhir.org.
authors: Nicolás
authors: N
authors: Fissolo
authors: Xavier
authors: X
authors: Montalban
authors: Manuel
authors: M
authors: Comabella
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000276:immunology; D001921:Brain / Q000276:immunology; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control; D006801:Humans; D007111:Immunity, Cellular; D051379:Mice; D009103:Multiple Sclerosis / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control; D009186:Myelin Sheath / Q000276:immunology; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*
chemical_list: D001324:Autoantigens; D016207:Cytokines; D019444:Vaccines, DNA