title: Renal ischaemia reperfusion injury: a mouse model of injury and regeneration.
abstract: Renal ischaemia reperfusion injury (IRI) is a common cause of acute kidney injury (AKI) in patients and occlusion of renal blood flow is unavoidable during renal transplantation. Experimental models that accurately and reproducibly recapitulate renal IRI are crucial in dissecting the pathophysiology of AKI and the development of novel therapeutic agents. Presented here is a mouse model of renal IRI that results in reproducible AKI. This is achieved by a midline laparotomy approach for the surgery with one incision allowing both a right nephrectomy that provides control tissue and clamping of the left renal pedicle to induce ischaemia of the left kidney. By careful monitoring of the clamp position and body temperature during the period of ischaemia this model achieves reproducible functional and structural injury. Mice sacrificed 24 hr following surgery demonstrate loss of renal function with elevation of the serum or plasma creatinine level as well as structural kidney damage with acute tubular necrosis evident. Renal function improves and the acute tissue injury resolves during the course of 7 days following renal IRI such that this model may be used to study renal regeneration. This model of renal IRI has been utilized to study the molecular and cellular pathophysiology of AKI as well as analysis of the subsequent renal regeneration.
authors: MRC Centre for Inflammation Research, University of Edinburgh; E.E.Hesketh@sms.ed.ac.uk.
authors: Emily E
authors: EE
authors: Hesketh
authors: MRC Centre for Inflammation Research, University of Edinburgh.
authors: Alicja
authors: A
authors: Czopek
authors: MRC Centre for Inflammation Research, University of Edinburgh.
authors: Michael
authors: M
authors: Clay
authors: MRC Centre for Inflammation Research, University of Edinburgh.
authors: Gary
authors: G
authors: Borthwick
authors: MRC Centre for Inflammation Research, University of Edinburgh.
authors: David
authors: D
authors: Ferenbach
authors: MRC Centre for Inflammation Research, University of Edinburgh.
authors: David
authors: D
authors: Kluth
authors: MRC Centre for Inflammation Research, University of Edinburgh.
authors: Jeremy
authors: J
authors: Hughes
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007511:Ischemia; D007668:Kidney / Q000098:blood supply*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015427:Reperfusion Injury