title: Activity of tea tree (Melaleuca alternifolia) essential oil against L3 larvae of Anisakis simplex.
abstract: Nematicidal activity of Melaleuca alternifolia essential oil, commonly known as tea tree oil (TTO), was assayed in vitro against L3 larvae of Anisakis simplex. The results showed a mortality of 100% for concentrations between 7 and 10 μL/mL after 48 h of incubation, obtaining an LD50 value of 4.53 μL/mL after 24 hours and 4.27 μL/mL after 48 hours. Concentration-dependent inhibition of acetylcholinesterase was observed for tea tree essential oil showing inhibition values of 100% at 100 μL/mL. This fact suggests that TTO may act as an AChE inhibitor. Terpinen-4-ol was discarded as main larvicide compound as it did not show larvicidal or anticholinesterase activity. The data obtained suggest that the essential oil of Melaleuca alternifolia may have a great therapeutic potential for the treatment of human anisakiasis.
authors: Facultad de Ciencias de la Salud, Universidad San Jorge, Campus Universitario Villanueva de Gállego, Autovia A-23 Zaragoza, Huesca, Km 299, 50.830 Villanueva de Gállego, Zaragoza, Spain.
authors: Carlota
authors: 0000-0003-3982-2282
authors: C
authors: Gómez-Rincón
authors: Facultad de Ciencias de la Salud, Universidad San Jorge, Campus Universitario Villanueva de Gállego, Autovia A-23 Zaragoza, Huesca, Km 299, 50.830 Villanueva de Gállego, Zaragoza, Spain.
authors: Elisa
authors: E
authors: Langa
authors: Facultad de Ciencias de la Salud, Universidad San Jorge, Campus Universitario Villanueva de Gállego, Autovia A-23 Zaragoza, Huesca, Km 299, 50.830 Villanueva de Gállego, Zaragoza, Spain.
authors: Paula
authors: P
authors: Murillo
authors: Facultad de Ciencias de la Salud, Universidad San Jorge, Campus Universitario Villanueva de Gállego, Autovia A-23 Zaragoza, Huesca, Km 299, 50.830 Villanueva de Gállego, Zaragoza, Spain.
authors: Marta Sofía
authors: 0000-0002-2231-7565
authors: MS
authors: Valero
authors: Facultad de Ciencias de la Salud, Universidad San Jorge, Campus Universitario Villanueva de Gállego, Autovia A-23 Zaragoza, Huesca, Km 299, 50.830 Villanueva de Gállego, Zaragoza, Spain.
authors: César
authors: 0000-0001-7783-6672
authors: C
authors: Berzosa
authors: Facultad de Ciencias de la Salud, Universidad San Jorge, Campus Universitario Villanueva de Gállego, Autovia A-23 Zaragoza, Huesca, Km 299, 50.830 Villanueva de Gállego, Zaragoza, Spain.
authors: Víctor
authors: 0000-0001-6969-1055
authors: V
authors: López
mesh_terms: D000818:Animals; D017163:Anisakis / Q000254:growth & development*; D000871:Anthelmintics / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007814:Larva / Q000254:growth & development; D031650:Melaleuca / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000871:Anthelmintics; D009822:Oils, Volatile