title: Protective efficacy of ursodeoxycholic acid nanoparticles in animal model of inflammatory bowel disease.
abstract: Abstract Context: Ursodeoxycholic acid (UDCA) exerts dose-dependent chemoprevention in colonic inflammation. Polycationic UDCA nanoparticles (UNPs) are envisaged for solubility enhancement and site directed drug delivery.
  
  
  OBJECTIVE
  The objective was to study the ameliorative efficacy of UNPs through localized delivery of the drug.
  
  
  METHODS
  UNPs were prepared through nanoprecipitation technique. Particle size, morphology, in vitro drug release and in vivo protective efficacy in inflammatory bowel disease (IBD) of these nanoparticles were studied.
  
  
  CONCLUSIONS
  The average particle size was around 100 nm, and the average drug encapsulation was about 99%. In vitro drug release study shows optimal drug release in simulated colonic fluid. The lowering of tissue nitric oxide, malondialdehyde, myeloperoxidase and histology of the colon tissue supported the protective efficacy of the nanoparticles.
  
  
  CONCLUSIONS
  This study presents the improved efficacy of UNPs in animal model of IBD due to complete release of drug at the desired site of action.
authors: Department of Chemical Technology, Division of Pharmaceuticals and Fine Chemical Technology, University College of Science and Technology, University of Calcutta , Kolkata , West Bengal , India.
authors: Amit Kumar
authors: AK
authors: De
authors: Santanu
authors: S
authors: Sana
authors: Sriparna
authors: S
authors: Datta
authors: Arup
authors: A
authors: Mukherjee
mesh_terms: D000818:Animals; D002756:Cholagogues and Choleretics / Q000737:chemistry / Q000494:pharmacology; D003106:Colon / Q000378:metabolism* / Q000473:pathology; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D009569:Nitric Oxide / Q000378:metabolism; D010316:Particle Size; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014580:Ursodeoxycholic Acid / Q000737:chemistry / Q000494:pharmacology
chemical_list: D002756:Cholagogues and Choleretics; D004337:Drug Carriers; D009569:Nitric Oxide; D008315:Malondialdehyde; D014580:Ursodeoxycholic Acid; D009195:Peroxidase
keywords: 2; 4; 6-trinitrobenzenesulfonic acid; Eudragit RS100 and RL100; factorial design; nanoprecipitation