title: Potential and limitations of genetic manipulation in animals.
abstract: Over the last decade, sequencing and characterisation of the mouse genome has been accompanied by unparalleled advances in functional genomics. In the context of drug action, we analyse the strengths and limitations of classical mutagenesis and gene targeting techniques, as well as alternative approaches such as chemical mutagenesis, gene trap, recombineering, transposon-mediated mutagenesis, chromosomal engineering, viral transgenesis and RNA interference. This review also focuses on the emerging importance of genetic manipulation in other species and related logistical issues of experimental work using mutants.:
authors: Molecular & Cellular Therapeutics and Research Institute, Royal College of Surgeons in Ireland, St. Stephen's Green, Dublin 2, Ireland.
authors: Gerard J
authors: GJ
authors: O'Sullivan
authors: Molecular & Cellular Therapeutics and Research Institute, Royal College of Surgeons in Ireland, St. Stephen's Green, Dublin 2, Ireland.
authors: Colm M
authors: CM
authors: O'Tuathaigh
authors: Molecular & Cellular Therapeutics and Research Institute, Royal College of Surgeons in Ireland, St. Stephen's Green, Dublin 2, Ireland.
authors: Jeremiah J
authors: JJ
authors: Clifford
authors: Molecular & Cellular Therapeutics and Research Institute, Royal College of Surgeons in Ireland, St. Stephen's Green, Dublin 2, Ireland.
authors: Gillian F
authors: GF
authors: O'Meara
authors: Molecular & Cellular Therapeutics and Research Institute, Royal College of Surgeons in Ireland, St. Stephen's Green, Dublin 2, Ireland.
authors: David T
authors: DT
authors: Croke
authors: Molecular & Cellular Therapeutics and Research Institute, Royal College of Surgeons in Ireland, St. Stephen's Green, Dublin 2, Ireland. Electronic address:jwadding@rcsi.ie.
authors: John L
authors: JL
authors: Waddington