title: Reversal of the β-oxidation cycle in Saccharomyces cerevisiae for production of fuels and chemicals.
abstract: Functionally reversing the β-oxidation cycle represents an efficient and versatile strategy for synthesis of a wide variety of fuels and chemicals. However, due to the compartmentalization of cellular metabolisms, reversing the β-oxidation cycle in eukaryotic systems remains elusive. Here, we report the first successful reversal of the β-oxidation cycle in Saccharomyces cerevisiae, an important cell factory for large-scale production of fuels and chemicals. After extensive gene cloning and enzyme activity assays, a reversed β-oxidation pathway was functionally constructed in the yeast cytosol, which led to the synthesis of n-butanol, medium-chain fatty acids (MCFAs), and medium-chain fatty acid ethyl esters (MCFAEEs). The resultant recombinant strain provides a new broadly applicable platform for synthesis of fuels and chemicals in S. cerevisiae.
authors: †Department of Chemical and Biomolecular Engineering, ‡Energy Biosciences Institute, Institute for Genomic Biology, §Departments of Chemistry, Biochemistry, and Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
authors: Jiazhang
authors: J
authors: Lian
authors: †Department of Chemical and Biomolecular Engineering, ‡Energy Biosciences Institute, Institute for Genomic Biology, §Departments of Chemistry, Biochemistry, and Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
authors: Huimin
authors: H
authors: Zhao
mesh_terms: D020001:1-Butanol / Q000032:analysis / Q000378:metabolism; D056804:Biofuels; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D010084:Oxidation-Reduction; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D056804:Biofuels; D004798:Enzymes; D005227:Fatty Acids; D011994:Recombinant Proteins; D020001:1-Butanol
keywords: advanced biofuels; fatty acid; synthetic biology; yeast; β-oxidation