title: Three-dimensional super-resolution and localization of dense clusters of single molecules.
abstract: When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to overlap, existing solutions to determine the number of molecules present and their precise three-dimensional locations can tolerate little to no overlap. We propose a localization scheme that can identify several overlapping molecule images while maintaining high localization precision. A solution to this problem involving matched optical and digital techniques, as here proposed, can substantially increase the allowable labeling density and accelerate the data collection time of single-molecule localization microscopy by more than one order of magnitude.
authors: Department of Electrical, Computer, and Energy Engineering, University of Colorado at Boulder UCB 425, Boulder, Colorado 80309, USA.
authors: Anthony
authors: A
authors: Barsic
authors: Department of Electrical, Computer, and Energy Engineering, University of Colorado at Boulder UCB 425, Boulder, Colorado 80309, USA.
authors: Ginni
authors: G
authors: Grover
authors: Department of Electrical, Computer, and Energy Engineering, University of Colorado at Boulder UCB 425, Boulder, Colorado 80309, USA.
authors: Rafael
authors: R
authors: Piestun