title: γ-Dodecelactone production from safflower oil via 10-hydroxy-12(Z)-octadecenoic acid intermediate by whole cells of Candida boidinii and Stenotrophomonas nitritireducens.
abstract: Candida boidinii was selected as a γ-dodecelactone producer because of the highest production of γ-dodecelactone from 10-hydroxy-12(Z)-octadecenoic acid among the 11 yeast strains tested. Under the reaction conditions of pH 5.5 and 25 °C with 5 g/L 10-hydroxy-12(Z)-octadecenoic acid and 30 g/L cells, whole C. boidinii cells produced 2.1 g/L γ-dodecelactone from 5 g/L 10-hydroxy-12(Z)-octadecenoic acid after 6 h, with a conversion yield of 64% (mol/mol) and a volumetric productivity of 350 mg/L/h. The production of γ-dodecelactone from safflower oil was performed by lipase hydrolysis reaction and two-step whole-cell biotransformation using Stenotrophomonas nitritireducens and C. boidinii. γ-Dodecelactone at 1.88 g/L was produced from 7.5 g/L safflower oil via 5 g/L 10-hydroxy-12(Z)-octadecenoic acid intermediate by these reactions after 8 h of reaction time, with a volumetric productivity of 235 mg/L/h and a conversion yield of 25% (w/w). To the best of the authors' knowledge, this is the highest volumetric productivity and conversion yield reported to date for the production of γ-lactone from natural oils.
authors: Department of Bioscience and Biotechnology, Konkuk University , Seoul 143-701, Republic of Korea.
authors: Ye-Seul
authors: YS
authors: Jo
authors: Jung-Ung
authors: JU
authors: An
authors: Deok-Kun
authors: DK
authors: Oh
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D002175:Candida / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism; D009829:Oleic Acids / Q000378:metabolism*; D012450:Safflower Oil / Q000378:metabolism*; D020588:Stenotrophomonas / Q000378:metabolism*; D013696:Temperature
chemical_list: D009829:Oleic Acids; D012450:Safflower Oil; C040430:10-hydroxy-12-octadecenoic acid; D008049:Lipase; D015107:4-Butyrolactone; C517611:gamma-dodecalactone