title: Polyphenol-rich strawberry extract protects human dermal fibroblasts against hydrogen peroxide oxidative damage and improves mitochondrial functionality.
abstract: Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging.
authors: Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, Via Ranieri 65, Ancona 60131, Italy. f.giampieri@univpm.it.
authors: Francesca
authors: F
authors: Giampieri
authors: Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, Via Ranieri 65, Ancona 60131, Italy. j.m.alvarez@univpm.it.
authors: José M
authors: JM
authors: Alvarez-Suarez
authors: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona 60131, Italy. l.mazzoni@univpm.it.
authors: Luca
authors: L
authors: Mazzoni
authors: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona 60131, Italy. tamara.forbe@gmail.com.
authors: Tamara Y
authors: TY
authors: Forbes-Hernandez
authors: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona 60131, Italy. m.gasparrini@univpm.it.
authors: Massimiliano
authors: M
authors: Gasparrini
authors: Grupo de Investigación en Polifenoles (GIP-USAL), Faculty of Pharmacy, Salamanca University, Campus Miguel de Unamuno, Salamanca E37007, Spain. paramas@usal.es.
authors: Ana M
authors: AM
authors: Gonzàlez-Paramàs
authors: Grupo de Investigación en Polifenoles (GIP-USAL), Faculty of Pharmacy, Salamanca University, Campus Miguel de Unamuno, Salamanca E37007, Spain. csb@usal.es.
authors: Celestino
authors: C
authors: Santos-Buelga
authors: Department of Physiology, Institute of Nutrition and Food Technology ''José Mataix", Biomedical Research Centre, University of Granada, Granada 18100, Spain. jlquiles@ugr.es.
authors: José L
authors: JL
authors: Quiles
authors: Dipartimento di Scienze Biomediche e Sanità Pubblica, Facoltà di Medicina, Università Politecnica delle Marche Via Ranieri 65, Ancona 60131, Italy. s.bompadre@univpm.it.
authors: Stefano
authors: S
authors: Bompadre
authors: Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, Via Ranieri 65, Ancona 60131, Italy. b.mezzetti@univpm.it.
authors: Bruno
authors: B
authors: Mezzetti
authors: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona 60131, Italy. m.a.battino@univpm.it.
authors: Maurizio
authors: M
authors: Battino
mesh_terms: D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000032:analysis; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D020405:Dermis / Q000166:cytology / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005419:Flavonoids / Q000032:analysis; D031985:Fragaria / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects* / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D019800:Phenol / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology; D019207:beta Carotene / Q000032:analysis
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D005419:Flavonoids; D009840:Oligomycins; D010936:Plant Extracts; D019207:beta Carotene; D019800:Phenol; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid