title: [Methicillin-sensitive Staphylococcus aureus isolates related to USA300 clone: Origin of community-genotype MRSA in Colombia?].
abstract: BACKGROUND
  USA300 is a genetic lineage found both in methicillin-resistant (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) isolates. In Colombia, hospital and community MRSA infections are caused by a USA300-related community genotype MRSA (CG-MRSA) clone. The genetic origin of this clone is unknown yet.
  
  
  OBJECTIVE
  To identify and characterize methicillin-resistant (MRSA) and methicillin-sensitive S. aureus (MSSA) isolates in order to improve the information about the origin of the CG-MRSA isolates in Colombia.
  
  
  METHODS
  USA300-related MSSA isolates were detected and characterized from a study of 184 S. aureus isolates (90 MRSA and 94 MSSA) recovered from infections. The genetic relatedness of the isolates was established by means of pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and protein A gene typification ( spa typing).
  
  
  RESULTS
  Among 184 isolates, 27 (14.7%) showed molecular characteristics and genetic relationship with the USA300 clone, of which 18 were MRSA and nine were MSSA. All USA300-related MRSA harbored Staphylococcal cassette chromosome mec (SCC mec ) IVc (3.1.2). In the MSSA isolates, SCC mec remnants or att B duplicate sites were not detected.
  
  
  CONCLUSIONS
  In Colombia, the CG-MRSA isolates probably originated in the dissemination of an USA300-related MSSA clone which later acquired SCC mec IVc.
authors: Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, D.C, Colombia.
authors: Javier Antonio
authors: JA
authors: Escobar-Pérez
authors: Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, D.C, Colombia.
authors: Betsy Esperanza
authors: BE
authors: Castro
authors: Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, D.C, Colombia.
authors: Ricaurte Alejandro
authors: RA
authors: Márquez-Ortiz
authors: Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, D.C, Colombia.
authors: Sebastián
authors: S
authors: Gaines
authors: Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, D.C, Colombia.
authors: Bibiana
authors: B
authors: Chavarro
authors: Grupo de Microbiología, Dirección de Investigación en Salud Pública, Subdirección de Investigación Científica y Tecnológica, Instituto Nacional de Salud, Bogotá, D.C, Colombia.
authors: Jaime
authors: J
authors: Moreno
authors: Grupo para el Control de la Resistencia Bacteriana en Bogotá, Universidad Nacional de Colombia, Bogotá, D.C, Colombia.
authors: Aura Lucía
authors: AL
authors: Leal
authors: I3 Institute, Faculty of Science, University of Technology Sydney.
authors: Natasha
authors: N
authors: Vanegas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002677:Chile; D002999:Clone Cells; D003105:Colombia / Q000453:epidemiology; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D008875:Middle Aged; D046915:Penicillin-Binding Proteins; D010802:Phylogeny; D011446:Prospective Studies; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D014481:United States; D014774:Virulence / Q000235:genetics; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus