title: Kinetin inhibits apoptosis of aging spleen cells induced by D-galactose in rats.
abstract: Kinetin (Kn) is a cytokinin growth factor that exerts several anti-aging and antioxidant effects on cells and organs. To investigate the mechanism underlying apoptotic events in aging cells induced by D-galactose (D-gal), we examined the effect of Kn delivered via nuchal subcutaneous injection on D-gal-induced aging and apoptosis in rats. Our results showed that interleukin (IL)-2 levels and mitochondrial membrane potential (ΔΨm) were decreased by Kn in aging rats while IL-6 production and apoptosis increased. In addition, the expression of anti-apoptotic Bcl-2 was low while that of Bax was high in the aging group. After treated with Kn, compared with aging group, there showed obvious difference in Kn group with elevated IL-2, proliferation index, Bcl-2, ΔΨm and decreased IL-6 and Bax in splenic lymphocyte. Based on these results, we concluded that Kn can effectively protect the rat spleen from aging, apoptosis, and atrophy.
authors: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
authors: Mengyun
authors: M
authors: Li
authors: Wuqing
authors: W
authors: Ouyang
authors: Xiaoli
authors: X
authors: Wu
authors: Yin
authors: Y
authors: Zheng
authors: Yunpeng
authors: Y
authors: Wei
authors: Lei
authors: L
authors: An
mesh_terms: D000375:Aging / Q000187:drug effects / Q000502:physiology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005690:Galactose / Q000494:pharmacology*; D015850:Interleukin-6 / Q000502:physiology; D007378:Interleukins / Q000502:physiology; D007701:Kinetin / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000502:physiology; D051381:Rats; D013154:Spleen / Q000166:cytology* / Q000187:drug effects / Q000502:physiology
chemical_list: D015850:Interleukin-6; D007378:Interleukins; D007701:Kinetin; D005690:Galactose
keywords: apoptosis; immunity; kinetin; mitochondrial membrane potential; spleen