title: [Determination of skimmin, scopolin and umbelliferone in Tibetan medicine Saussurea hieracioides by HPLC].
abstract: This study is aimed to establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of skimmin, scopolin and umbelliferone in Saussurea hieracioides. Samples were analyzed on a Wondasil C18-WR column (4.6 mm x 250 mm, 5 microm) with methanol (A) and water containing 0.1% phosphate (B) as mobile phases for gradient elution at a flow rate of 1.0 mL x min(-1). The detection wavelength and column temperature were set at 325 nm and 35 degrees C, respectively, and the sample size was 10 microL. The results showed that skimmin, scopolin and umbelliferone were simultaneously achieved within 40 min under the above conditions. A good linearity was observed in the range of 0.18-5.6 microg (r = 1.000 0), 0.060-1.8 microg (r = 0.999 9), 0.032-0.97 microg (r = 0.999 8) for skimmin, scopolin and umbelliferone, respectively, with the average recoveries of 99.16% (RSD = 0.41%), 100.3% (RSD = 0.79%), 102.2% (RSD = 0.87%). The method is simple, accurate and reproducible and can be used for the quality control of S. hieracioides.
authors: Rong
authors: R
authors: Tan
authors: Yu-Jie
authors: YJ
authors: Wang
authors: Yu-Xin
authors: YX
authors: Zhang
authors: Li-Shi
authors: LS
authors: Zhou
authors: Er
authors: E
authors: Tan
authors: A
authors: A
authors: Ping
authors: Yi
authors: Y
authors: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D038861:Medicine, Tibetan Traditional; D015203:Reproducibility of Results; D030441:Saussurea / Q000737:chemistry*; D014468:Umbelliferones / Q000032:analysis*
chemical_list: D003374:Coumarins; D005960:Glucosides; D014468:Umbelliferones; C417572:scopolin; C031477:7-hydroxycoumarin; C581268:skimmin