title: The antibiotic and membrane-damaging activities of cellobiose lipids and sophorose lipids.
abstract: Antibiotic activity was compared for Cryptococcus humicola cellobiose lipids, the mixture of 2,3,4-О-triacetyl-β-D-glucopyranosyl-(1→4)-(6-О-acetyl-β-D-glucopyranosyl-(1→16)-2,16-dihydroxyhexodecanoic acid and 2,3,4-О-triacetyl-β-D-glucopyranosyl-(1→4)-(6-О-acetyl-β-D-glucopyranosyl-(1→16)-2,17,18-trihydroxyoctotodecanoic acid, and the commercial sophorose lipid mixture of a mono-acetylated acidic sophorose lipid and a di-acetylated acidic sophorose lipid, both containing the C18:1 fatty acid residue. The MIC values of cellobiose lipids were 0.005 and 0.04 mg/mL for Filobasidiella neoformans and Candida tropicalis, respectively. The MIC values of sophorose lipids were 1 and 15 mg/mL for F. neoformans and C. tropicalis, respectively. MIC values for some bacteria were in the range of 10-30 mg/mL for both glycolipid preparations. Both sophorose lipids and cellobiose lipids displayed a membrane-damaging activity against F. neoformans. The treatment with these glycolipids reduces the content of ATP in the cells of test cultures and results in their staining with ethidium bromide.
authors: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences.
authors: Ekaterina
authors: E
authors: Kulakovskaya
authors: Boris
authors: B
authors: Baskunov
authors: Anton
authors: A
authors: Zvonarev
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D041022:Candida tropicalis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects*; D002475:Cellobiose / Q000737:chemistry / Q000494:pharmacology*; D003455:Cryptococcus neoformans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D024881:Drug Resistance, Bacterial; D025141:Drug Resistance, Fungal; D005936:Glucans / Q000737:chemistry / Q000494:pharmacology*; D008055:Lipids / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D004338:Drug Combinations; D005936:Glucans; D008055:Lipids; D002475:Cellobiose; D000255:Adenosine Triphosphate; C003328:sophorose