title: Rapid assessment of oxygen transfer impact for Corynebacterium glutamicum.
abstract: Oxygen supply is crucial in industrial application of microbial systems, such as Corynebacterium glutamicum, but oxygen transfer is often neglected in early strain characterizations, typically done under aerobic conditions. In this work, a new procedure for oxygen transfer screening is presented, assessing the impact of maximum oxygen transfer conditions (OTRmax) within microtiter plate-based cultivation for enhanced throughput. Oxygen-dependent growth and productivity were characterized for C. glutamicum ATCC13032 and C. glutamicum DM1933 (lysine producer). Biomass and lysine product yield are affected at OTRmax below 14 mmol L(-1) h(-1) in a standardized batch process, but not by further increase of OTRmax above this threshold value indicating a reasonable tradeoff between power input and oxygen transfer capacity OTRmax. The described oxygen transfer screening allows comparative determination of metabolic robustness against oxygen transfer limitation and serves identification of potential problems or opportunities later created during scale-up.
authors: Forschungszentrum Jülich GmbH, Institute of Bio- and Geosciences, IBG-1: Biotechnology, 52425, Jülich, Germany.
authors: Friedrich
authors: F
authors: Käß
authors: Arjun
authors: A
authors: Prasad
authors: Jana
authors: J
authors: Tillack
authors: Matthias
authors: M
authors: Moch
authors: Heiner
authors: H
authors: Giese
authors: Jochen
authors: J
authors: Büchs
authors: Wolfgang
authors: W
authors: Wiechert
authors: Marco
authors: M
authors: Oldiges
mesh_terms: D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002138:Calibration; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D005285:Fermentation; D005740:Gases; D008239:Lysine / Q000737:chemistry*; D009862:Online Systems; D010100:Oxygen / Q000737:chemistry*
chemical_list: D005740:Gases; D008239:Lysine; D010100:Oxygen