title: Increase in gene-transcript levels as indicators of up-regulation of the unfolded protein response in spontaneous canine tumors.
abstract: The unfolded protein response (UPR), a conserved cellular response to stressors such as hypoxia and nutrient deprivation, is associated with angiogenesis and metastasis in tumor cells. This article discusses a pilot study conducted to determine whether components of the UPR could be identified in spontaneous canine tumors and whether they were up-regulated within tumor tissue compared with adjacent normal tissue. Tissue samples of various spontaneous canine neoplasms were taken from 13 dogs shortly after surgical excision or euthanasia; control samples were taken from adjacent normal tissue. RNA purification and real-time quantitative reverse-transcription polymerase chain reaction were done to measure the expression of 4 genes associated with the UPR (HERP, CHOP, GRP78, and XBP1s). The results indicated that UPR gene expression can be identified in spontaneous canine tumors and that the UPR is up-regulated, as indicated by significantly increased expression of CHOP and GRP78 within the tumor.
authors: Department of Small Animal Clinical Sciences (Elliot, MacDonald-Dickinson, Linn), Department of Veterinary Pathology (Simko), and Department of Veterinary Microbiology (Misra) Western College of Veterinary Medicine, 52 Campus Road, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B5.
authors: Kirsten
authors: K
authors: Elliot
authors: Department of Small Animal Clinical Sciences (Elliot, MacDonald-Dickinson, Linn), Department of Veterinary Pathology (Simko), and Department of Veterinary Microbiology (Misra) Western College of Veterinary Medicine, 52 Campus Road, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B5.
authors: Valerie
authors: V
authors: MacDonald-Dickinson
authors: Department of Small Animal Clinical Sciences (Elliot, MacDonald-Dickinson, Linn), Department of Veterinary Pathology (Simko), and Department of Veterinary Microbiology (Misra) Western College of Veterinary Medicine, 52 Campus Road, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B5.
authors: Kathleen
authors: K
authors: Linn
authors: Department of Small Animal Clinical Sciences (Elliot, MacDonald-Dickinson, Linn), Department of Veterinary Pathology (Simko), and Department of Veterinary Microbiology (Misra) Western College of Veterinary Medicine, 52 Campus Road, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B5.
authors: Elemir
authors: E
authors: Simko
authors: Department of Small Animal Clinical Sciences (Elliot, MacDonald-Dickinson, Linn), Department of Veterinary Pathology (Simko), and Department of Veterinary Microbiology (Misra) Western College of Veterinary Medicine, 52 Campus Road, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B5.
authors: Vikram
authors: V
authors: Misra
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology / Q000601:surgery / Q000662:veterinary*; D010865:Pilot Projects; D012313:RNA / Q000235:genetics / Q000378:metabolism; D056811:Unfolded Protein Response / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D009363:Neoplasm Proteins; D012313:RNA