title: Transcriptional response to vancomycin in a highly vancomycin-resistant Streptomyces coelicolor mutant.
abstract: OBJECTIVE
  The main objective of this study is to understand the mechanism of vancomycin resistance in a Streptomyces coelicolor disrupted mutant highly resistant to vancomycin.
  
  
  METHODS
  Different techniques have been performed in the study including gene disruption, primer extension, antibiotic susceptibility tests, electron microscopy, confocal microscopy, cell wall analysis and microarrays.
  
  
  RESULTS
  During the phenotypical characterization of mutant strains affected in phosphate-regulated genes of unknown function, we found that the S. coelicolor SCO2594 disrupted mutant was highly resistant to vancomycin and had other phenotypic alterations such as antibiotic overproduction, impaired growth and reduction of phosphate cell wall content. Transcriptomic studies with this mutant indicated a relationship between vancomycin resistance and cell wall stress.
  
  
  CONCLUSIONS
  We identified a S. coelicolor mutant highly resistant to vancomycin in both high and low phosphate media. In addition to Van proteins, others such as WhiB or SigE appear to be involved in this regulatory mechanism.
authors: Instituto de Biotecnología de León (INBIOTEC), Avda. Real 1, 24006 León, Spain.
authors: Fernando
authors: F
authors: Santos-Beneit
authors: Lorena T
authors: LT
authors: Fernández-Martínez
authors: Antonio
authors: A
authors: Rodríguez-García
authors: Antonio
authors: A
authors: Rodríguez García
authors: Seomara
authors: S
authors: Martín-Martín
authors: María
authors: M
authors: Ordóñez-Robles
authors: Paula
authors: P
authors: Yagüe
authors: Angel
authors: A
authors: Manteca
authors: Juan F
authors: JF
authors: Martín
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002473:Cell Wall / Q000235:genetics / Q000473:pathology*; D003428:Cross Infection / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010710:Phosphates / Q000737:chemistry; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000187:drug effects* / Q000235:genetics*; D013312:Stress, Physiological / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010710:Phosphates; D012808:Sigma Factor; D014157:Transcription Factors; C111259:sigE protein, Bacteria; C081543:whiB protein, Streptomyces; D014640:Vancomycin
keywords: PhoP; SCO2594; Streptomyces ; cell wall; glycopeptides; phosphate; teicoplanin; vancomycin