title: Stable mixed hematopoietic chimerism permits tolerance of vascularized composite allografts across a full major histocompatibility mismatch in swine.
abstract: This study tested the hypothesis that vascularized composite allografts (VCA) could be accepted in a robust model of hematopoietic chimerism by injecting allogeneic bone marrow cells (BMC) into swine fetuses. Outbred Yorkshire sows and boars were screened to ensure the absence of the major histocompatibility (MHC) allele SLA(cc) of inbred MGH miniature swine and then mated. Bone marrow harvested from an SLA(cc) swine donor was T-cell depleted and injected intravenously into the fetuses between days 50-55 of gestation. After birth, the piglets were studied with flow cytometry to detect donor cells and mixed lymphocyte reactions (MLR) and cell-mediated lympholysis (CML) assays to assess their response to donor. Donor-matched VCAs from SLA(cc) donors were performed on four chimeric and two nonchimeric swine. The results showed donor cell engraftment and multilineage macrochimerism after the in utero transplantation of adult BMC, and chimeric animals were unresponsive to donor antigens in vitro. Both control VCAs were rejected by 21 days and were alloreactive. Chimeric animals accepted the VCAs and never developed antidonor antibodies or alloreactivity to donor. These results confirm that the intravascular, in utero transplantation of adult BMC leads to donor cell chimerism and donor-specific tolerance of VCAs across a full MHC barrier in this animal model.
authors: Division of Plastic Surgery, University of Colorado, School of Medicine, Denver, CO, USA.
authors: David W
authors: DW
authors: Mathes
authors: Mario G
authors: MG
authors: Solari
authors: Guy Scott
authors: GS
authors: Gazelle
authors: Peter E M
authors: PE
authors: Butler
authors: Anette
authors: A
authors: Wu
authors: Adam
authors: A
authors: Nazzal
authors: Gunnlauger P
authors: GP
authors: Nielsen
authors: Christene A
authors: CA
authors: Huang
authors: David H
authors: DH
authors: Sachs
authors: Wei Ping Andrew
authors: WP
authors: Lee
authors: Mark A
authors: MA
authors: Randolph
mesh_terms: D064591:Allografts; D000818:Animals; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000379:methods; D064595:Composite Tissue Allografts / Q000098:blood supply* / Q000637:transplantation; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006084:Graft Rejection; D006085:Graft Survival; D006648:Histocompatibility; D007108:Immune Tolerance / Q000502:physiology; D007959:Lymphocyte Culture Test, Mixed; D011247:Pregnancy; D011270:Pregnancy, Animal; D011897:Random Allocation; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D016038:Skin Transplantation / Q000009:adverse effects / Q000379:methods; D013552:Swine; D013556:Swine, Miniature; D018183:Transplantation Chimera / Q000276:immunology*; D023001:Transplantation Tolerance / Q000502:physiology*
keywords: bone marrow; chimera; fetal; swine; vascularized composite allograft