title: Analysis of cross-reactive neutralizing antibodies in human HFMD serum with an EV71 pseudovirus-based assay.
abstract: Hand, foot and mouth disease, associated with enterovirus 71 (EV71) infections, has recently become an important public health issue throughout the world. Serum neutralizing antibodies are major indicators of EV71 infection and protective immunity. However, the potential for cross-reactivity of neutralizing antibodies for different EV71 genotypes and subgenotypes is unclear. Here we measured the cross-reactive neutralizing antibody titers against EV71 of different genotypes or subgenotypes in sera collected from EV71-infected children and vaccine-inoculated children in a phase III clinical trial (ClinicalTrials.gov Identifier: NCT01636245) using a new pseudovirus-based neutralization assay. Antibodies induced by EV71-C4a were cross-reactive for different EV71 genotypes, demonstrating that C4a is a good candidate strain for an EV71 vaccine. Our study also demonstrated that this new assay is practical for analyses of clinical samples from epidemiological and vaccine studies.
authors: School of Life Sciences, Jilin University, Changchun, P. R. China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, P. R. China.
authors: Huafei
authors: H
authors: Zhang
authors: School of Life Sciences, Jilin University, Changchun, P. R. China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, P. R. China.
authors: Dong
authors: D
authors: An
authors: School of Life Sciences, Jilin University, Changchun, P. R. China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, P. R. China.
authors: Wei
authors: W
authors: Liu
authors: National Institutes for Food and Drug Control, Beijing, P. R. China.
authors: Qunying
authors: Q
authors: Mao
authors: School of Life Sciences, Jilin University, Changchun, P. R. China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, P. R. China.
authors: Jun
authors: J
authors: Jin
authors: School of Life Sciences, Jilin University, Changchun, P. R. China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, P. R. China.
authors: Lin
authors: L
authors: Xu
authors: School of Life Sciences, Jilin University, Changchun, P. R. China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, P. R. China.
authors: Shiyang
authors: S
authors: Sun
authors: School of Life Sciences, Jilin University, Changchun, P. R. China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, P. R. China.
authors: Liping
authors: L
authors: Jiang
authors: School of Life Sciences, Jilin University, Changchun, P. R. China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, P. R. China.
authors: Xiaojun
authors: X
authors: Li
authors: National Institutes for Food and Drug Control, Beijing, P. R. China.
authors: Jie
authors: J
authors: Shao
authors: Henan Provincial Center for Disease Control and Prevention, Zhengzhou, P. R. China.
authors: Hongxia
authors: H
authors: Ma
authors: Henan Provincial Center for Disease Control and Prevention, Zhengzhou, P. R. China.
authors: Xueyong
authors: X
authors: Huang
authors: Department of Pediatrics, the First Hospital of Jilin University, Changchun, P. R. China.
authors: Shijie
authors: S
authors: Guo
authors: Department of Pediatrics, the First Hospital of Jilin University, Changchun, P. R. China.
authors: Haiying
authors: H
authors: Chen
authors: National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Science, Xiamen University, Xiamen, P. R. China.
authors: Tong
authors: T
authors: Cheng
authors: National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Science, Xiamen University, Xiamen, P. R. China.
authors: Lisheng
authors: L
authors: Yang
authors: School of Life Sciences, Jilin University, Changchun, P. R. China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, P. R. China; Key Laboratory for Molecular Enzymology & Engineering, Ministry of Education, Jilin University, Changchun, P. R. China.
authors: Weiheng
authors: W
authors: Su
authors: School of Life Sciences, Jilin University, Changchun, P. R. China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, P. R. China; Key Laboratory for Molecular Enzymology & Engineering, Ministry of Education, Jilin University, Changchun, P. R. China.
authors: Wei
authors: W
authors: Kong
authors: National Institutes for Food and Drug Control, Beijing, P. R. China.
authors: Zhenglun
authors: Z
authors: Liang
authors: School of Life Sciences, Jilin University, Changchun, P. R. China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, P. R. China; Key Laboratory for Molecular Enzymology & Engineering, Ministry of Education, Jilin University, Changchun, P. R. China.
authors: Chunlai
authors: C
authors: Jiang
mesh_terms: D057134:Antibodies, Neutralizing / Q000097:blood* / Q000276:immunology*; D002675:Child, Preschool; D003429:Cross Reactions; D029821:Enterovirus A, Human / Q000235:genetics / Q000502:physiology*; D005838:Genotype; D057809:HEK293 Cells; D006232:Hand, Foot and Mouth Disease / Q000097:blood* / Q000276:immunology / Q000821:virology*; D006801:Humans; D049409:Luciferases, Firefly / Q000235:genetics; D009500:Neutralization Tests; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D014765:Viral Vaccines; D049409:Luciferases, Firefly