title: Possible role of nitric oxide in hepatic injury secondary to renal ischemia-reperfusion (I/R) injury.
abstract: Hepatic injury secondary to renal I/R injury has been documented in several studies. This study aimed to investigate the role of NO in hepatic injury secondary to renal I/R in rat model. Sprague-Dawley rats (n = 48) were divided into 4 equal groups; sham-operated, I/R injury group (45 min of bilateral renal ischemia), L-arginine group (I/R with 300 mg/kg L-arginine, 20 min before ischemia), L-NAME group (I/R with 50 mg/kg L-NAME, 20 min before ischemia). L-NAME (NO synthase inhibitor) caused significant elevation in serum creatinine, BUN, liver enzymes, liver histopathological damage score (p ≤ 0.05) and MDA production (p ≤ 0.001); on the other hand significantly decreased NO and GSH levels (p ≤ 0.05). L-arginine significantly decreased serum creatinine, BUN and GSH (p ≤ 0.05) and caused significant elevation in liver enzymes and NO (p ≤ 0.05), and also in MDA levels (p ≤ 0.001) in liver tissues. We conclude that endogenous NO might have protective effect against hepatic injury induced by renal I/R injury and inhibition of this endogenous NO by L-NAME or exogenous administration of NO (by L-arginine) might be harmful.
authors: Medical Physiology Department, Mansoura Faculty of Medicine, Mansoura, Egypt. menhag@mans.edu.eg.
authors: Abdelaziz M
authors: AM
authors: Hussein
authors: Hazem K
authors: HK
authors: Khaled
authors: Mohamed O
authors: MO
authors: Seisa
authors: Azza
authors: A
authors: Baiomy
authors: Mie A
authors: MA
authors: Mohamed
authors: Dina
authors: D
authors: Eltantawy
authors: Amel A
authors: AA
authors: Mahmoud
authors: Hussein A
authors: HA
authors: Sheashaa
authors: Mohamed A
authors: MA
authors: Sobh
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002374:Catalase / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000293:injuries* / Q000473:pathology; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications*
chemical_list: D009569:Nitric Oxide; D003404:Creatinine; D002374:Catalase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione