title: Detection and spread of oxa-48-producing Klebsiella oxytoca isolates in Istanbul, Turkey.
abstract: Five OXA-48 producing Klebsiella oxytoca strains isolated in April-July 2010 were analyzed. Antibiotic susceptibility tests were performed using disc diffusion method and VITEK 2 system. Carbapenemase activity was investigated using the Modified Hodge test. Beta-lactamase genes were detected by PCR and blaOXA-48 was sequenced. Genetic relatedness between K. oxytoca isolates was investigated by pulse-field gel electrophoresis (PFGE). Carbapenemase activity was detected in 5 isolates by Modified Hodge test. Although all strains were resistant to ertapenem and imipenem, only one strain was also resistant to meropenem. BlaOXA-48 in 4 isolates harbored 2 or 3 other ESBL types, namely, blaTEM, blaSHV, blaCTX-M, or blaVEB. PFGE revealed 3 different pulso-types among the K. oxytoca isolates. The presence of OXA-48 carbapenemase in other species of clinical isolates should also be considered.
authors: Hasan
authors: H
authors: Nazik
authors: Selda
authors: S
authors: Aydin
authors: Rüveyda
authors: R
authors: Albayrak
authors: Esma A
authors: EA
authors: Bilgi
authors: Ismail
authors: I
authors: Yildiz
authors: Nuray
authors: N
authors: Kuvat
authors: Fatih M
authors: FM
authors: Kelesoglu
authors: Fatih M
authors: FM
authors: Kelesoglu
authors: Nagehan
authors: N
authors: Pakaştiçali
authors: Fadime
authors: F
authors: Yilmaz
authors: Betigül
authors: B
authors: Ongen
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D007223:Infant; D007710:Klebsiella Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission*; D041121:Klebsiella oxytoca / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D016896:Treatment Outcome; D014421:Turkey
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems