title: Overexpression of IL-9 induced by STAT6 activation promotes the pathogenesis of chronic lymphocytic leukemia.
abstract: Chronic lymphocytic leukemia (CLL) is a common leukemia in adults, but its pathogenesis is still poorly understood. Recently, extensive evidence suggests that the malignant cells of CLL patients secrete a range of cytoprotective cytokines including interleukin-4 (IL-4). IL-4 induced the rapid phosphorylation(p) and activation of the signal transducer and activator of transcription (STAT)-6 transcription factor in CLL cells in vitro. Interleukin-9 (IL-9) is not expressed by Th2 and Th9 cells in the absence of STAT6 expression. To elucidate whether there was a function link between IL-9 and STAT6 in CLL, MEC-1 cells were analyzed using RT-PCR, and western blot. Interestingly, when added with recombinant human IL-4 (rIL-4) in culturing MEC-1 cells, expressions of p-STAT6 and IL-9 in MEC-1 cells increased at a time-dependent manner and their expressions could be inhibited by STAT6 inhibitor. Our data indicated that the upregulation of IL-9 induced by pSTAT6 may be involved in the pathogenesis of CLL.
authors: Department of Hematology, Provincial Hospital Affiliated to Shandong University Jinan, Shandong, China.
authors: Na
authors: N
authors: Chen
authors: Department of Hematology, Provincial Hospital Affiliated to Shandong University Jinan, Shandong, China.
authors: Kang
authors: K
authors: Lu
authors: Department of Hematology, Provincial Hospital Affiliated to Shandong University Jinan, Shandong, China.
authors: Peipei
authors: P
authors: Li
authors: Department of Hematology, Provincial Hospital Affiliated to Shandong University Jinan, Shandong, China.
authors: Xiao
authors: X
authors: Lv
authors: Department of Hematology, Provincial Hospital Affiliated to Shandong University Jinan, Shandong, China ; Institute of Diagnostics, School of Medicine, Shandong University Jinan, Shandong, China.
authors: Xin
authors: X
authors: Wang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D016906:Interleukin-9 / Q000235:genetics / Q000378:metabolism*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D050800:STAT6 Transcription Factor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation
chemical_list: C508598:IL4 protein, human; C508607:IL9 protein, human; D016906:Interleukin-9; D012333:RNA, Messenger; D011994:Recombinant Proteins; D050800:STAT6 Transcription Factor; C494101:STAT6 protein, human; D015847:Interleukin-4
keywords: chronic lymphocytic leukemia; pSTAT6; prognosis