title: Effects of supra-zero storage on human ovarian cortex prior to vitrification-warming.
abstract: This study investigated the effects of supra-zero storage on ovarian cortex of 12 premenopausal patients before cryopreservation. Fresh ovarian tissue (control) was either vitrified immediately or stored at 4°C for 24 h or 48 h before vitrification. This study assessed malondialdehyde release during storage and the capacity to synthesize oestradiol in culture, follicle morphology and the proportion of apoptotic follicles subsequent to vitrification-warming. The malondialdehyde concentration in the storage medium increased significantly in a time-dependent manner (P = 0.029). Oestradiol release during tissue culture decreased statistically significantly in both storage groups compared with the immediate vitrification group (P = 0.043). The proportion of high-quality follicles was significantly different between the fresh group and the two storage groups (24 h, P < 0.01; 48 h, P < 0.001). The proportion of apoptotic follicles was significantly different between the fresh and immediate vitrification groups (P < 0.05). The 24-h and 48-h groups were not different compared with the other groups. This study provides evidence that storage at supra-zero conditions has detrimental effects on the human ovarian cortex. Storage duration should therefore be limited to a minimum to prevent potential damage.
authors: Department of Gynecological Endocrinology and Reproductive Medicine, University Hospital of Schleswig-Holstein, Ratzeburger Allee 160, 23538 Lübeck, Germany. Electronic address: silke.klocke@web.de.
authors: Silke
authors: S
authors: Klocke
authors: Department of Gynecological Endocrinology and Reproductive Medicine, University Hospital of Schleswig-Holstein, Ratzeburger Allee 160, 23538 Lübeck, Germany.
authors: Claudia
authors: C
authors: Tappehorn
authors: Department of Gynecological Endocrinology and Reproductive Medicine, University Hospital of Schleswig-Holstein, Ratzeburger Allee 160, 23538 Lübeck, Germany.
authors: Georg
authors: G
authors: Griesinger
mesh_terms: D015925:Cryopreservation; D003470:Culture Media; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D010053:Ovary / Q000378:metabolism / Q000502:physiology*; D011247:Pregnancy; D013696:Temperature; D058989:Vitrification
chemical_list: D003470:Culture Media; D004958:Estradiol; D008315:Malondialdehyde
keywords: human ovarian cortex; malondialdehyde; oestradiol; transportation; vitrification