title: Frequency of CD4+CXCR5+ TFH cells in patients with hepatitis b virus-associated membranous nephropathy.
abstract: The frequency of different subsets of CD4(+)CXCR5(+) TFH cells and serum cytokine levels were analyzed in a total of 14 patients with newly diagnosed hepatitis B virus-associated membranous nephropathy (HBV-MN), 12 individuals with immune-tolerant HBV infection (HBV-IT) and 12 healthy controls (HC). Serum cytokine levels were measured before and 10-12 weeks after treatment. Significantly higher frequency of CD4(+)CXCR5(+), CD4(+)CXCR5(+)ICOS(+) and CD4(+)CXCR5(+)PD-1(+) TFH cells, and higher serum levels of IL-17A, IFN-γ, IL-2, IL-10, IL-4 and IL-21 were detected in HBV-MN patients compared to the HC. The percentage of CD4(+)CXCR5(+) TFH cells and serum IL-21 level in HBV-MN patients were also higher than the HBV-IT. The percentage of CD4(+)CXCR5(+) TFH cell was negatively correlated with the value of eGFR, and the percentage of CD4(+)CXCR5(+)ICOS(+) TFH cells was positively correlated with the 24-h urinary protein concentration. Notably, the percentage of CD4(+)CXCR5(+)PD-1(+) TFH cells was positively correlated with serum IL-21 level and 24-h urinary protein concentration. Treatment with prednisone or/and immunosuppressive drugs significantly reduced the frequency of CD4(+)CXCR5(+), CD4(+)CXCR5(+)PD-1(+) TFH cells and serum IL-21 level, but increased IL-4 and IL-10 levels in the patients. CD4(+)CXCR5(+) TFH cells, especially CD4(+)CXCR5(+)PD-1(+) TFH cells may participate in the pathogenesis of HBV-MN.
authors: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital of Jilin University, Changchun, 130032, China.
authors: Yong
authors: Y
authors: Liu
authors: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital of Jilin University, Changchun, 130032, China.
authors: Pingwei
authors: P
authors: Zhao
authors: Department of Nephrology, The First Hospital of Jilin University, Changchun 130021, China.
authors: Zhihui
authors: Z
authors: Qu
authors: Department of Medical Laboratory Sciences, Haramaya University, Dire Dawa, Ethiopia.
authors: Desalegn Admassu
authors: DA
authors: Ayana
authors: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital of Jilin University, Changchun, 130032, China. Electronic address: yanfangjiang@hotmail.com.
authors: Yanfang
authors: Y
authors: Jiang
mesh_terms: D000328:Adult; D015704:CD4 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D015433:Glomerulonephritis, Membranous / Q000188:drug therapy / Q000209:etiology / Q000276:immunology*; D006509:Hepatitis B / Q000150:complications / Q000188:drug therapy / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology*; D006801:Humans; D007108:Immune Tolerance; D016130:Immunophenotyping; D007166:Immunosuppressive Agents / Q000008:administration & dosage; D060889:Inducible T-Cell Co-Stimulator Protein / Q000378:metabolism; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D011241:Prednisone / Q000008:administration & dosage; D061026:Programmed Cell Death 1 Receptor / Q000378:metabolism; D054380:Receptors, CXCR5 / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D055815:Young Adult
chemical_list: D015704:CD4 Antigens; D016207:Cytokines; C556692:ICOS protein, human; D007166:Immunosuppressive Agents; D060889:Inducible T-Cell Co-Stimulator Protein; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; D054380:Receptors, CXCR5; D011241:Prednisone
keywords: HBV-MN; IL-21; IL-4; TFH cell