title: Exposure to submicron particles (PM1.0) from diesel exhaust and pollen allergens of human lung epithelial cells induces morphological changes of mitochondria tonifilaments and rough endoplasmic reticulum.
abstract: In recent literature, little has been said regarding the morphological changes that occur in lung cells after treatment with particles and nanoparticles. Using an in vitro model of type-II lung epithelium (A549), we studied the effects of submicron particles (PM1.0), Parietaria officinalis (ALL), and PM1.0 + ALL together. To date several biochemical effects have been described, instead few data exist in literature regarding morphological events following these treatments, in particular we focused on the morphological changes and distribution of mitochondria, tonifilaments and rough endoplasmic reticulum, using a transmission electron microscopic (TEM) approach. After exposure to PM1.0 particles (PM1.0), Parietaria officinalis as allergen, and PM1.0 with P. officinalis, changes in the cytoplasmic area were observed, such as damage to mitochondria and morphological alterations of the tonifilaments and rough endoplasmic reticulum. The data obtained strongly support the hypothesis that cells in contact with submicron particles (PM1.0), or P. officinalis, undergo alteration of their metabolism.
authors: Department of Cardiothoracic and Respiratory Sciences, Second University of Naples, Naples, Italy.
authors: Gennaro
authors: G
authors: Mazzarella
authors: Section of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, Second University of Naples, Naples, Italy.
authors: Angela
authors: A
authors: Lucariello
authors: Department of Health Sciences, Faculty of Medicine and Surgery, University of Molise, Campobasso, Italy.
authors: Andrea
authors: A
authors: Bianco
authors: Department of Cardiothoracic and Respiratory Sciences, Second University of Naples, Naples, Italy.
authors: Cecilia
authors: C
authors: Calabrese
authors: Section of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, Second University of Naples, Naples, Italy.
authors: Theodoros
authors: T
authors: Thanassoulas
authors: Section of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, Second University of Naples, Naples, Italy.
authors: Leonilde
authors: L
authors: Savarese
authors: Section of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, Second University of Naples, Naples, Italy.
authors: Angelamaria
authors: A
authors: Fiumarella
authors: Section of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, Second University of Naples, Naples, Italy.
authors: Vincenzo
authors: V
authors: Esposito
authors: Section of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, Second University of Naples, Naples, Italy Antonio.deluca@unina2.it.
authors: Antonio
authors: A
authors: DE Luca
mesh_terms: D000485:Allergens / Q000009:adverse effects* / Q000633:toxicity; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018870:Endoplasmic Reticulum, Rough / Q000648:ultrastructure; D004847:Epithelial Cells / Q000187:drug effects* / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D008928:Mitochondria / Q000648:ultrastructure; D052638:Particulate Matter / Q000633:toxicity*; D011058:Pollen / Q000009:adverse effects* / Q000633:toxicity; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D000485:Allergens; D052638:Particulate Matter; D001335:Vehicle Emissions
keywords: A549 cell line; Human epithelial airway model; TEM; particles; submicron PM