title: 30μm spatial resolution protein MALDI MSI: In-depth comparison of five sample preparation protocols applied to human healthy and atherosclerotic arteries.
abstract: Tissue preparation is the key to a successful MALDI mass spectrometry imaging experiment. A number of different tissue preparations methods have recently been reported for increased sensitivity and/or high spatial resolution analysis. In order to better benchmark these methods in terms of the information content and their suitability for analyzing small tissues containing small but distinct regions, we have performed an extensive comparison using technical and biological repeats as well as a fully randomized measuring sequence. We then demonstrate how the optimized tissue preparation method enables 30μm spatial resolution analysis of proteins from atherosclerotic arterial tissues, revealing proteins specific to the intima and media layers.
authors: Department of Immunology, IIS-Fundacion Jimenez Diaz, Madrid, Spain.
authors: Marta
authors: M
authors: Martin-Lorenzo
authors: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
authors: Benjamin
authors: B
authors: Balluff
authors: Department of Immunology, IIS-Fundacion Jimenez Diaz, Madrid, Spain.
authors: Aroa
authors: A
authors: Sanz-Maroto
authors: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
authors: René J M
authors: RJ
authors: van Zeijl
authors: Department of Immunology, IIS-Fundacion Jimenez Diaz, Madrid, Spain; Department of Biochemistry and Molecular Biology I, Universidad Complutense, Madrid, Spain.
authors: Fernando
authors: F
authors: Vivanco
authors: Department of Immunology, IIS-Fundacion Jimenez Diaz, Madrid, Spain.
authors: Gloria
authors: G
authors: Alvarez-Llamas
authors: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: L.A.Mcdonnell@lumc.nl.
authors: Liam A
authors: LA
authors: McDonnell
mesh_terms: D001158:Arteries / Q000378:metabolism / Q000473:pathology; D050197:Atherosclerosis / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D057054:Molecular Imaging / Q000379:methods*; D009124:Muscle Proteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D017539:Tunica Intima / Q000378:metabolism* / Q000473:pathology; D017540:Tunica Media / Q000378:metabolism* / Q000473:pathology
chemical_list: D009124:Muscle Proteins
keywords: Atherosclerosis; High spatial resolution; MALDI mass spectrometry imaging; Sample preparation