title: Identification of polyphenols from antiviral Chamaecrista nictitans extract using high-resolution LC-ESI-MS/MS.
abstract: Chamaecrista nictitans (L) extract possesses antiviral properties; it acts against the herpes simplex virus, and this may be attributed to its constituent phenolics. Here, high-resolution LC-ESI-MS/MS is used to identify the phenolic components of the most potent fraction of the extract. The fraction is a complex mixture rich in oligomeric proanthocyanidins with a high content of monohydroxyphenol moieties ((epi)fisetinidol, (epi)afzelechin and (epi)guibourtinidol) and A-type linkages, uncommon in other proanthocyanidin-rich phenolic extracts, such as those from grape seeds or pine bark. As monohydroxyphenolic structures and A-type linkages have been related to antiviral effects, particularly through the inhibition of late transcription, we suggest that the fraction of C. nictitans extract exerts its action through a particularly effective combination of proanthocyanidins that include these two structural features.
authors: Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), Jordi Girona 18-26, 08034, Barcelona, Spain.
authors: María Luisa
authors: ML
authors: Mateos-Martín
authors: Elisabet
authors: E
authors: Fuguet
authors: Allan
authors: A
authors: Jiménez-Ardón
authors: Libia
authors: L
authors: Herrero-Uribe
authors: Giselle
authors: G
authors: Tamayo-Castillo
authors: Josep Lluís
authors: JL
authors: Torres
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry*; D031288:Chamaecrista / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D002853:Chromatography, Liquid; D004279:DNA, Viral / Q000737:chemistry; D047309:Flavones / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D016166:Free Radical Scavengers; D006561:Herpes Simplex / Q000188:drug therapy / Q000517:prevention & control; D006801:Humans; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013329:Structure-Activity Relationship; D053719:Tandem Mass Spectrometry
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D047309:Flavones; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D044945:Proanthocyanidins; C013221:proanthocyanidin; C510796:afzelechin