title: Second-harmonic generation imaging of cancer.
abstract: The last 30 years has seen great advances in optical microscopy with the introduction of sophisticated fluorescence-based imaging methods such as confocal and multiphoton laser scanning microscopy. There is increasing interest in using these methods to quantitatively examine sources of intrinsic biological contrast including autofluorescent endogenous proteins and light interactions such as second-harmonic generation (SHG) in collagen. In particular, SHG-based microscopy has become a widely used quantitative modality for imaging noncentrosymmetric proteins such as collagen in a diverse range of tissues. Due to the underlying physical origin of the SHG signal, it is highly sensitive to collagen fibril/fiber structure and, importantly, to collagen-associated changes that occur in diseases such as cancer, fibrosis, and connective tissue disorders. An overview of SHG physics background and technologies is presented with a focused review on applications of SHG primarily as applied to cancer.
authors: Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison, USA.
authors: Adib
authors: A
authors: Keikhosravi
authors: Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison, USA.
authors: Jeremy S
authors: JS
authors: Bredfeldt
authors: Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison, USA.
authors: Abdul Kader
authors: AK
authors: Sagar
authors: Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison, USA.
authors: Kevin W
authors: KW
authors: Eliceiri
mesh_terms: D000818:Animals; D005260:Female; D024022:Fibrillar Collagens / Q000648:ultrastructure; D005454:Fluorescence Polarization; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D061848:Optical Imaging / Q000379:methods; D010051:Ovarian Neoplasms / Q000175:diagnosis*; D012878:Skin Neoplasms / Q000175:diagnosis*
chemical_list: D024022:Fibrillar Collagens
keywords: Cancer; Cells; Imaging; Imaging biomarkers; Second-harmonic generation; Tumor