title: Antepartal insulin-like growth factor concentrations indicating differences in the metabolic adaptive capacity of dairy cows.
abstract: Cows with different Insulin-like Growth Factor-I (IGF-I) concentrations showed comparable expression levels of hepatic growth hormone receptor (GHR). Suppressor of cytokine signaling 2 (SOCS2), could be responsible for additional inhibition of the GHR signal cascade. The aims were to monitor cows with high or low antepartal IGF-I concentrations (IGF-I(high) or IGF-I(low)), evaluate the interrelationships of endocrine endpoints, and measure hepatic SOCS2 expression. Dairy cows (n = 20) were selected (240 to 254 days after artificial insemination (AI)). Blood samples were drawn daily (day -17 until calving) and IGF-I, GH, insulin, thyroid hormones, estradiol, and progesterone concentrations were measured. Liver biopsies were taken (day 264 ± 1 after AI and postpartum) to measure mRNA expression (IGF-I, IGFBP-2, IGFBP-3, IGFBP-4, acid labile subunit (ALS), SOCS2, deiodinase1, GHR1A). IGF-I concentrations in the two groups were different (p < 0.0001). However, GH concentrations and GHR1A mRNA expression were comparable (p > 0.05). Thyroxine levels and ALS expression were higher in the IGF-I(high) cows compared to IGF-I(low) cows. Estradiol concentration tended to be greater in the IGF-I(low) group (p = 0.06). It was hypothesized that low IGF-I levels are associated with enhanced SOCS2 expression although this could not be decisively confirmed by the present study.
authors: Clinic for Cattle, University of Veterinary Medicine, 30173 Hannover, Germany.marion.piechotta@tiho-hannover.de.
authors: Marion
authors: M
authors: Piechotta
authors: Lars
authors: L
authors: Holzhausen
authors: Marcelo Gil
authors: MG
authors: Araujo
authors: Maike
authors: M
authors: Heppelmann
authors: Anja
authors: A
authors: Sipka
authors: Chistiane
authors: C
authors: Pfarrer
authors: Hans-Joachim
authors: HJ
authors: Schuberth
authors: Heinrich
authors: H
authors: Bollwein
mesh_terms: D000818:Animals; D002417:Cattle; D004958:Estradiol / Q000097:blood; D005260:Female; D013006:Growth Hormone / Q000097:blood; D007328:Insulin / Q000097:blood; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000032:analysis; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000032:analysis; D018974:Insulin-Like Growth Factor Binding Protein 4 / Q000032:analysis; D007334:Insulin-Like Growth Factor I / Q000032:analysis* / Q000502:physiology; D008099:Liver / Q000737:chemistry; D011247:Pregnancy / Q000378:metabolism / Q000502:physiology; D011270:Pregnancy, Animal / Q000378:metabolism* / Q000502:physiology; D011374:Progesterone / Q000097:blood; D050826:Suppressor of Cytokine Signaling Proteins / Q000032:analysis; D013963:Thyroid Hormones / Q000097:blood
chemical_list: D007328:Insulin; D018971:Insulin-Like Growth Factor Binding Protein 2; D018972:Insulin-Like Growth Factor Binding Protein 3; D018974:Insulin-Like Growth Factor Binding Protein 4; D050826:Suppressor of Cytokine Signaling Proteins; D013963:Thyroid Hormones; D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone
keywords: cattle; growth hormone; insulin-like growth factor I; metabolism; suppressor of cytokine signaling