title: Essential enrichment strategies in phosphoproteomics.
abstract: Reversible protein phosphorylation ranks among the most important post-translational modifications that occur in the cell. Therefore, the characterization of phosphorylation events is a highly relevant albeit challenging task. Significant progress surrounding the biochemical analysis of reversible protein phosphorylation in the past 2 years has led to the development of several new techniques to isolate or enrich phosphopeptides, particularly in large-scale analyses. This review gives an overview of the most frequently used phosphopeptide enrichment methods.:
authors: Department of Biomolecular Mass Spectrometry, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.
authors: Martijn W H
authors: MW
authors: Pinkse
authors: Department of Biomolecular Mass Spectrometry, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands. Electronic address:a.j.r.heck@chem.uu.nl.
authors: Albert J R
authors: AJ
authors: Heck