title: The influence of vitamin-rich diet on the extent of lipoperoxidation in brain of mice during an acute post-insulin hypoglycaemia.
abstract: Antioxidatives are widely used and recommended in common clinical praxis, even though they may have negative impact on our health under some circumstances (i.e. N-acetylcysteine, vitamin E, risk of lung cancer etc.). Our aim was to evaluate the role of exogenous scavengers in prevention of induced oxidative stress in rodents. Male ICR mice were used and acute hypoglycaemia was induced with insulin. The mice were randomized into eight experimental groups, either pretreated by vitamin C or vitamin E or combinations with respective vehicles. Total malondialdehyde (MDA), superoxide dismutase (SOD), and selenium-dependent glutathione peroxidase (GSHPx) activity were measured in brain tissue samples. ANOVA with a post-hoc Duncan or Turkey׳s tests were used for statistical evaluation. A statistically significant increase in brain MDA was observed after insulin-induced severe hypoglycaemia relative to normoglycaemia. Animals pretreated with vitamins, both in monotherapy and in combination (both P<0.05), had significantly lower MDA values compared with animals without pretreatment. Importantly, significant differences were also observed after combination of vitamin C and E in GSHPx and SOD (both P<0.05).
authors: Department of Pharmacology, 3rd Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague 10, Czech Republic.
authors: Jitka
authors: J
authors: Patockova
authors: Department of Pharmacology, 3rd Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague 10, Czech Republic; Department of Pharmacology, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic. Electronic address: jiri.sliva@lf3.cuni.cz.
authors: Jiri
authors: J
authors: Sliva
authors: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Petr
authors: P
authors: Marhol
authors: Department of Chemistry, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
authors: Jirina
authors: J
authors: Crkovska
authors: Department of Chemistry, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
authors: Stanislav
authors: S
authors: Stipek
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D001786:Blood Glucose / Q000032:analysis; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D019587:Dietary Supplements; D004359:Drug Therapy, Combination; D005979:Glutathione Peroxidase / Q000378:metabolism; D007003:Hypoglycemia / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D007328:Insulin; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008813:Mice, Inbred ICR; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D014810:Vitamin E; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid
keywords: Glutathione peroxidase; Hypoglycaemia; Lipoperoxidation; Malondialdehyde; Malondialdehyde (PubChem CID 10964); Superoxide dismutase; Vitamin C; Vitamin E