title: Real-time 3D particle manipulation visualized using volume holographic gratings.
abstract: Holographic optical tweezers (HOTs) extend optical trapping into three dimensions. Volume imaging then becomes a concern as trapped objects are easily moved out of focus of the imaging objective lens. Here we demonstrate a three-dimensional real-time interactive optical trapping, manipulating, and imaging system based on HOTs incorporated with volume holographic microscope. Intensity information about the trapped objects at multiple depths can be captured in a single measurement. This method is compatible with most imaging modes such as bright-field and fluorescence.
authors: Zhi
authors: Z
authors: Chen
authors: Wensheng
authors: W
authors: Chen
authors: Hsin-yu
authors: HY
authors: Lu
authors: Yves
authors: Y
authors: Chevallier
authors: Nanguang
authors: N
authors: Chen
authors: George
authors: G
authors: Barbastathis
authors: Yuan
authors: Y
authors: Luo
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D052898:Optical Tweezers; D012031:Refractometry / Q000295:instrumentation*