title: A novel nanogel-based fluorescent probe for ratiometric detection of intracellular pH values.
abstract: A new nanogel indicator (NGI) for ratiometric fluorescent detection of intracellular pH values has been reported. In aqueous solution, the NGI can be used for ratiometric sensing of pH changes with a large hypsochromic shift of 100 nm in the green-red region and reversible. Furthermore, the NGI has been used to calibrate the cytosol pH value in living cells.
authors: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. gqyang@iccas.ac.cn shayuli@iccas.ac.cn.
authors: Lixia
authors: L
authors: Cao
authors: Xiaoyan
authors: X
authors: Li
authors: Shuangqing
authors: S
authors: Wang
authors: Shayu
authors: S
authors: Li
authors: Yi
authors: Y
authors: Li
authors: Guoqiang
authors: G
authors: Yang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002477:Cells / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D051379:Mice; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D011874:Radiometry / Q000379:methods; D012996:Solutions
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D000080385:Nanogels; D012996:Solutions; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine