title: Characterization of dengue virus serotype 4 infection in Jakarta, Indonesia.
abstract: Dengue hemorrhagic fever has become a worldwide health issue. Heterologous infection by different serotypes may lead to severe forms of dengue infection and even death. In a cohort study in Jakarta from 2009 to 2010 with inclusion criteria of adults with fever of less than 48 hours, 72% were confirmed dengue infection from a total of suspected 190 dengue patients. Using RT-PCR, 16 patients were infected with DENV-4 with or without co-infection with other serotype(s). Dengue fever (DF) patients (73%) were infected with DENV-4 alone, while mixed infections were present in more severe clinical manifestations of DHF grade I and II. The nucleotide sequences of envelope (E) protein gene of 3 isolates of DENV-4 showed that they belonged to genotype II. There were 50 nucleotide substitutions, but only 3 amino acid changes were found at positions 130, 233 and 455, present in E protein isolated from DHF grade II patient.
authors: Beti Ernawati
authors: BE
authors: Dewi
authors: Leonard
authors: L
authors: Naiggolan
authors: Dwi Hilda
authors: DH
authors: Putri
authors: Novia
authors: N
authors: Rachmayanti
authors: Sarah
authors: S
authors: Albar
authors: Nadia Tita
authors: NT
authors: Indriastuti
authors: Wellyzar
authors: W
authors: Sjamsuridzal
authors: T Mirawati
authors: TM
authors: Sudiro
mesh_terms: D001483:Base Sequence; D060085:Coinfection; D003715:Dengue / Q000453:epidemiology* / Q000821:virology*; D003716:Dengue Virus / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D008297:Male; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping