title: Type II collagen and gelatin from silvertip shark (Carcharhinus albimarginatus) cartilage: isolation, purification, physicochemical and antioxidant properties.
abstract: Type II acid soluble collagen (CIIA), pepsin soluble collagen (CIIP) and type II gelatin (GII) were isolated from silvertip shark (Carcharhinus albimarginatus) cartilage and examined for their physicochemical and antioxidant properties. GII had a higher hydroxyproline content (173 mg/g) than the collagens and cartilage. CIIA, CIIP and GII were composed of two identical α1 and β chains and were characterized as type II. Amino acid analysis of CIIA, CIIP and GII indicated imino acid contents of 150, 156 and 153 amino acid residues per 1000 residues, respectively. Differing Fourier transform infrared (FTIR) spectra of CIIA, CIIP and GII were observed, which suggested that the isolation process affected the secondary structure and molecular order of collagen, particularly the triple-helical structure. The denaturation temperature of GII (32.5 °C) was higher than that of CIIA and CIIP. The antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl radicals and the reducing power of CIIP was greater than that of CIIA and GII. SEM microstructure of the collagens depicted a porous, fibrillary and multi-layered structure. Accordingly, the physicochemical and antioxidant properties of type II collagens (CIIA, CIIP) and GII isolated from shark cartilage were found to be suitable for biomedical applications.
authors: Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. srijeevithan@gmail.com.
authors: Elango
authors: E
authors: Jeevithan
authors: Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. bbao@shou.edu.cn.
authors: Bin
authors: B
authors: Bao
authors: Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. bruce.bu@unilever.com.
authors: Yongshi
authors: Y
authors: Bu
authors: Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. yzhou0724@163.com.
authors: Yu
authors: Y
authors: Zhou
authors: Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. bobozhao123@163.com.
authors: Qingbo
authors: Q
authors: Zhao
authors: Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. whwu@shou.edu.cn.
authors: Wenhui
authors: W
authors: Wu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002356:Cartilage / Q000737:chemistry*; D002942:Circular Dichroism; D024043:Collagen Type II / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D017433:Protein Structure, Secondary; D012754:Sharks / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity
chemical_list: D000975:Antioxidants; D024043:Collagen Type II; D005780:Gelatin