title: MicroRNA signature for HER2-positive breast and gastric cancer.
abstract: The molecular mechanism for aggressive clinical behaviour related to v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2) amplification is not fully-understood. In particular, little is known about microRNAs in the human epidermal growth factor receptor 2 (HER2) signaling network.
  
  
  
  Using microRNA microarray, the microRNA profiles of 16 HER2-positive breast carcinomas were compared with those of five luminal-type breast carcinomas. Additionally, two frozen, ERBB2-amplified gastric carcinomas were compared with their adjacent normal tissue samples. MicroRNAs that were differentially expressed according to the HER2 status in breast and gastric carcinomas were identified as the HER2 microRNA signature.
  
  
  
  MiR-337 and miR-302f were commonly overexpressed in HER2-postive breast and gastric cancer. MiR-139 and miR-129 were commonly underexpressed in HER2-positive breast and gastric cancer. A concordant pattern of microRNA expression was noted between discovery sets and the majority of candidate microRNAs (two out of three) in three validation sets.
  
  
  
  Our study identified novel microRNAs that were differentially expressed according to the HER2 status across different tumor types.
authors: National Cancer Center, Goyang, Republic of Korea.
authors: Han Sung
authors: HS
authors: Kang
authors: National Cancer Center, Goyang, Republic of Korea.
authors: Sang-Geun
authors: SG
authors: Jang
authors: Keimyung University DongSan Medical Center, Daegu, Republic of Korea.
authors: Sun Young
authors: SY
authors: Kwon
authors: Asan Medical Center, Seoul, Republic of Korea.
authors: Young Soo
authors: YS
authors: Park
authors: Laboratory of Cancer Biology and Genetics, National Cancer Institute, Bethesda, MD, U.S.A.
authors: Jeffrey E
authors: JE
authors: Green
authors: National Cancer Center, Goyang, Republic of Korea jungsro@ncc.re.kr hkim@ncc.re.kr.
authors: Hark Kyun
authors: HK
authors: Kim
authors: National Cancer Center, Goyang, Republic of Korea jungsro@ncc.re.kr hkim@ncc.re.kr.
authors: Jungsil
authors: J
authors: Ro
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000201:enzymology* / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D025341:Principal Component Analysis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human
keywords: HER2; breast cancer; gastric cancer; miR-129; miR-139