title: Docetaxel loaded chitosan nanoparticles: formulation, characterization and cytotoxicity studies.
abstract: The primary objective of the present investigation was to explore biodegradable chitosan as a polymeric material for formulating docetaxel nanoparticles (DTX-NPs) to be used as a delivery system for breast cancer treatment. Docetaxel loaded chitosan nanoparticles were formulated by water-in-oil nanoemulsion system and characterized in terms of particle size, zeta potential, polydispersity index, drug entrapment efficiency (EE), loading capacity (LC), scanning electron microscopy (SEM), in vitro release study and drug release kinetics. Further, to evaluate the potential anticancer efficacy of docetaxel loaded chitosan nanoparticulate system, in vitro cytotoxicity studies on human breast cancer cell line (MDA-MB-231) were carried out. The morphological studies revealed the spherical shape of docetaxel loaded chitosan nanoparticles having an average size of 170.1±5.42-227.6±7.87nm, polydispersity index in the range of 0.215±0.041-0.378±0.059 and zeta potential between 28.3 and 31.4mV. Nanoparticles exhibited 65-76% of drug entrapment and 8-12% loading capacity releasing about 68-83% of the drug within 12h following Higuchi's square-root kinetics. An increase of 20% MDA-MB-231 cell line growth inhibition was determined by docetaxel loaded chitosan nanoparticles with respect to the free drug after 72h incubation.
authors: Department of Pharmaceutics, Chandigarh College of Pharmacy, Mohali 140 110, India. Electronic address: ankitjain505@yahoo.in.
authors: Ankit
authors: A
authors: Jain
authors: Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar 125 001, India.
authors: Kanika
authors: K
authors: Thakur
authors: Department of Pharmaceutics, Chandigarh College of Pharmacy, Mohali 140 110, India.
authors: Preeti
authors: P
authors: Kush
authors: Department of Pharmaceutics, Chandigarh College of Pharmacy, Mohali 140 110, India.
authors: Upendra K
authors: UK
authors: Jain
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000737:chemistry*; D000077143:Docetaxel; D004337:Drug Carriers / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry*; D017830:Octoxynol / Q000737:chemistry; D009821:Oils / Q000737:chemistry; D010316:Particle Size; D013501:Surface-Active Agents / Q000737:chemistry; D043823:Taxoids / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D009821:Oils; D013501:Surface-Active Agents; D043823:Taxoids; D014867:Water; D000077143:Docetaxel; D017830:Octoxynol; D048271:Chitosan; D005976:Glutaral
keywords: Chitosan; Docetaxel; Nanoemulsion; Nanoparticles