title: Role of cytokine gene polymorphisms in gastric cancer risk in Chile.
abstract: OBJECTIVE
  To assess the role of pro- and anti-inflammatory polymorphisms in gastric cancer susceptibility.
  
  
  METHODS
  We genotyped 12 polymorphisms in eight cytokine genes (Interleukin-1β -IL1B-, IL8, IL17A, IL17F, IL32, tumor necrosis factor-α -TNF-, IL1RN, IL10) in a case-control study of 147 patients with gastric cancer and 172 controls.
  
  
  RESULTS
  Single polymorphism analysis revealed an association between the IL10 -592C>A single nucleotide polymorphism and cases with moderately- or well-differentiated tumors [AA vs. GG, odds ratio (OR)=3.01; 95% confidence interval (CI)=1.08-8.50]. We further analyzed gene-gene interactions using a combined attribute network implemented in multifactor dimensionality reduction software. The analysis revealed an interaction between IL8 -251A>T and IL32 rs28372698 SNPs among cases with moderately- or well-differentiated tumors. Homozygosity for both IL8 -251T and IL32 T alleles increases the odds for developing gastric cancer up to 2.63-fold (OR=2.63; 95% CI=1.15-6.03). This association was higher compared to the homozygosity for the IL8-251 T allele alone (OR=1.11; 95% CI=0.51-2.43) or the IL32 T allele alone (OR=1.21; 95% CI=0.54-2.72).
  
  
  CONCLUSIONS
  These findings suggest that IL10 -592C>A increases the odds for developing gastric cancer. An interaction between IL8 -251A>T and IL32 rs28372698 SNPs is also proposed.
authors: Human Genetics Program, Institute of Biomedical Sciences, School of Medicine, University of Chile, Santiago, Chile pgonzalez@med.uchile.cl.
authors: Patricio
authors: P
authors: Gonzalez-Hormazabal
authors: Department of Gastroenterology, University of Chile Clinical Hospital, Santiago, Chile Department of Surgery, University of Chile Clinical Hospital, Santiago, Chile.
authors: Maher
authors: M
authors: Musleh
authors: Department of Surgery, School of Medicine at Eastern Campus, University of Chile, Santiago, Chile.
authors: Marco
authors: M
authors: Bustamante
authors: Department of Surgery, San Juan de Dios Hospital, Santiago, Chile.
authors: Juan
authors: J
authors: Stambuk
authors: Department of Gastroenterology, University of Chile Clinical Hospital, Santiago, Chile.
authors: Susana
authors: S
authors: Escandar
authors: Department of Gastroenterology, University of Chile Clinical Hospital, Santiago, Chile Department of Surgery, University of Chile Clinical Hospital, Santiago, Chile.
authors: Hector
authors: H
authors: Valladares
authors: Department of Gastroenterology, University of Chile Clinical Hospital, Santiago, Chile Department of Surgery, University of Chile Clinical Hospital, Santiago, Chile.
authors: Enrique
authors: E
authors: Lanzarini
authors: Department of Surgery, Barros Luco Hospital, Santiago, Chile.
authors: Hector
authors: H
authors: Chiong
authors: Department of Surgery, University of Chile Clinical Hospital, Santiago, Chile.
authors: Jorge
authors: J
authors: Rojas
authors: Human Genetics Program, Institute of Biomedical Sciences, School of Medicine, University of Chile, Santiago, Chile.
authors: V Gonzalo
authors: VG
authors: Castro
authors: Human Genetics Program, Institute of Biomedical Sciences, School of Medicine, University of Chile, Santiago, Chile.
authors: Cristian
authors: C
authors: Rubio-Reyes
authors: Human Genetics Program, Institute of Biomedical Sciences, School of Medicine, University of Chile, Santiago, Chile.
authors: Lilian
authors: L
authors: Jara
authors: Department of Gastroenterology, University of Chile Clinical Hospital, Santiago, Chile.
authors: Zoltan
authors: Z
authors: Berger
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D016022:Case-Control Studies; D002677:Chile; D016207:Cytokines / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007378:Interleukins / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000235:genetics* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D016207:Cytokines; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha
keywords: Gastric cancer; SNP; cytokine; interaction; interleukin; polymorphisms