title: Chemical constituents of Meconopsis horridula and their simultaneous quantification by high-performance liquid chromatography coupled with tandem mass spectrometry.
abstract: Meconopsis horridula Hook.f. Thoms has been used as a traditional Tibetan medicine to clear away heat, relieve pain, and mobilize static blood. In this study, a reliable method based on high-performance liquid chromatography with diode array detection and electrospray ionization quadrupole time-of-flight tandem mass spectrometry was established for the identification of components in this herb. A total of 40 compounds (including 17 flavonoids, 15 alkaloids, and eight phenylpropanoids) were identified or tentatively identified. Among them, 17 components were identified in the herb for the first time. Compound 39 appears to be a novel compound, which is confirmed as 3-(kaempferol-8-yl)-2,3-epoxyflavanone by NMR spectroscopy and mass spectrometry. Moreover, seven major constituents were simultaneously quantified by the developed high-performance liquid chromatography with tandem triple-quadrupole mass spectrometry method. The quantitative method was validated and quality parameters were established. The study provides a comprehensive approach for understanding this herbal medicine.
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, China.
authors: Jiajia
authors: J
authors: Liu
authors: Haimei
authors: H
authors: Wu
authors: Feng
authors: F
authors: Zheng
authors: Wenyuan
authors: W
authors: Liu
authors: Feng
authors: F
authors: Feng
authors: Ning
authors: N
authors: Xie
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D015394:Molecular Structure; D028101:Papaveraceae / Q000737:chemistry*; D010666:Phenylpropionates / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D000470:Alkaloids; D005419:Flavonoids; D010666:Phenylpropionates
keywords: Chemical constituents; High-performance liquid chromatography; Meconopsis horridula; Simultaneous quantification; Tandem mass spectrometry