title: [Effect of spermatozoa from different sources on normal fertilization of oocytes and embryo quality and development in intracytoplasmic sperm injection cycles].
abstract: OBJECTIVE
  To evaluate the impact of spermatozoa from different sources on normal fertilization of oocytes, embryo quality and embryo developmental potential in intracytoplasmic sperm injection (ICSI) cycles.
  
  
  METHODS
  A retrospective analysis was conducted among 197 patients undergoing ICSI cycles in our center. The patients were classified into 3 groups according to the sources of semen, namely ejaculated spermatozoa group (n=102), percutaneous epididymal sperm aspiration (PESA) group (n=68), and testicular sperm aspiration (TESA) group (n=27). The ejaculated spermatozoa group was further classified into oligoasthenoteratozoospermia (n=67) and cryptozoospermia (n=35) subgroups. The normal fertilization, high-quality embryo, implantation and clinical pregnancy rates were compared among the groups; the rate of high-quality blastocyst formation in in-vitro culture of non-top quality embryos was also observed.
  
  
  RESULTS
  The patients with PESA showed significantly higher normal fertilization rate (75.6%) than those in oligoasthenoteratozoospermia (64.8%), cryptozoospermia (62.1%), and TESA (61.6%) groups (P<0.05). No significant differences were found in the high-quality embryo, implantation, and clinical pregnancy rates among the groups (P>0.05). The rate of high-quality blastocyst formation in the in-vitro culture of non-top quality embryos was also comparable among the groups (P>0.05).
  
  
  CONCLUSIONS
  Although spermatozoa obtained with by PESA is associated with a higher normal fertilization rate, the sources of spermatozoa do not significantly affect the embryonic quality and developmental potential in ICSI cycles.
authors: Center of Reproductive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: xie.duo18@gmail.com.
authors: Duo
authors: D
authors: Xie
authors: Zhuolin
authors: Z
authors: Qiu
authors: Chen
authors: C
authors: Luo
authors: Qingjun
authors: Q
authors: Chu
authors: Song
authors: S
authors: Quan
mesh_terms: D053627:Asthenozoospermia; D010064:Embryo Implantation; D047108:Embryonic Development; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D009845:Oligospermia; D009865:Oocytes; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D012661:Semen; D020554:Sperm Injections, Intracytoplasmic; D053625:Sperm Retrieval; D013094:Spermatozoa