title: Identification of dirty necrosis in colorectal carcinoma based on multiphoton microscopy.
abstract: Dirty necrosis within glandular lumina is often considered as a characteristic of colorectal carcinomas (CRCs) that is a diagnostically useful feature of CRCs with DNA microsatellite instability (MSI). Multiphoton microscopy (MPM), which is based on the second-harmonic generation and two-photon excited fluorescence signals, was used to identify dirty necrosis. Our results demonstrated that MPM has the ability to exhibit the microstructure of dirty necrosis and the signal intensity as well as an emission spectrum that can help to differentiate dirty necrosis from cancer cells. These findings indicate that MPM may be helpful in distinguishing MSI colorectal carcinoma via the identification of dirty necrosis.
authors: Fujian Normal University, Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fuzhou 350007, China.
authors: Lianhuang
authors: L
authors: Li
authors: Fujian Medical University, The Affiliated Union Hospital, Department of Colorectal Surgery, Fuzhou 350001, China.
authors: Weizhong
authors: W
authors: Jiang
authors: Fujian Medical University, The Affiliated Union Hospital, Department of Pathology, Fuzhou 350001, China.
authors: Yinghong
authors: Y
authors: Yang
authors: Fujian Medical University, The Affiliated Union Hospital, Department of Colorectal Surgery, Fuzhou 350001, China.
authors: Zhifen
authors: Z
authors: Chen
authors: Fujian Medical University, The Affiliated Union Hospital, Department of Pathology, Fuzhou 350001, China.
authors: Changyin
authors: C
authors: Feng
authors: Fujian Normal University, Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fuzhou 350007, China.
authors: Hongsheng
authors: H
authors: Li
authors: Fujian Medical University, The Affiliated Union Hospital, Department of Colorectal Surgery, Fuzhou 350001, China.
authors: Guoxian
authors: G
authors: Guan
authors: Fujian Normal University, Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fuzhou 350007, China.
authors: Jianxin
authors: J
authors: Chen
mesh_terms: D003094:Collagen / Q000737:chemistry; D003110:Colonic Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D004548:Elasticity; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology; D007834:Lasers; D036641:Microscopy, Fluorescence, Multiphoton; D009336:Necrosis; D012004:Rectal Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D012007:Rectum / Q000473:pathology; D015203:Reproducibility of Results
chemical_list: D003094:Collagen