title: Application of fractal and grey level co-occurrence matrix analysis in evaluation of brain corpus callosum and cingulum architecture.
abstract: This aim of this study was to assess the discriminatory value of fractal and grey level co-occurrence matrix (GLCM) analysis methods in standard microscopy analysis of two histologically similar brain white mass regions that have different nerve fiber orientation. A total of 160 digital micrographs of thionine-stained rat brain white mass were acquired using a Pro-MicroScan DEM-200 instrument. Eighty micrographs from the anterior corpus callosum and eighty from the anterior cingulum areas of the brain were analyzed. The micrographs were evaluated using the National Institutes of Health ImageJ software and its plugins. For each micrograph, seven parameters were calculated: angular second moment, inverse difference moment, GLCM contrast, GLCM correlation, GLCM variance, fractal dimension, and lacunarity. Using the Receiver operating characteristic analysis, the highest discriminatory value was determined for inverse difference moment (IDM) (area under the receiver operating characteristic (ROC) curve equaled 0.925, and for the criterion IDM≤0.610 the sensitivity and specificity were 82.5 and 87.5%, respectively). Most of the other parameters also showed good sensitivity and specificity. The results indicate that GLCM and fractal analysis methods, when applied together in brain histology analysis, are highly capable of discriminating white mass structures that have different axonal orientation.
authors: 1Institute of Medical Physiology,School of Medicine,University of Belgrade,Visegradska 26/II,11129,Belgrade,Serbia.
authors: Igor
authors: I
authors: Pantic
authors: 2Institute of Physiology and Biochemistry, Faculty of Biology,University of Belgrade,Studentski trg 3,11000,Belgrade,Serbia.
authors: Sanja
authors: S
authors: Dacic
authors: 1Institute of Medical Physiology,School of Medicine,University of Belgrade,Visegradska 26/II,11129,Belgrade,Serbia.
authors: Predrag
authors: P
authors: Brkic
authors: 3Department of Neurobiology,Institute for Biological Research "Sinisa Stankovic",University of Belgrade,Boulevard Despot Stefan 142,11060 Belgrade,Serbia.
authors: Irena
authors: I
authors: Lavrnja
authors: 4Institute of Histology,School of Medicine,University of Belgrade,Visegradska 26/II,11129,Belgrade,Serbia.
authors: Senka
authors: S
authors: Pantic
authors: 1Institute of Medical Physiology,School of Medicine,University of Belgrade,Visegradska 26/II,11129,Belgrade,Serbia.
authors: Tomislav
authors: T
authors: Jovanovic
authors: 3Department of Neurobiology,Institute for Biological Research "Sinisa Stankovic",University of Belgrade,Boulevard Despot Stefan 142,11060 Belgrade,Serbia.
authors: Sanja
authors: S
authors: Pekovic
mesh_terms: D000818:Animals; D003337:Corpus Callosum / Q000033:anatomy & histology*; D006179:Gyrus Cinguli / Q000033:anatomy & histology*; D006651:Histocytochemistry / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D012372:ROC Curve; D051381:Rats; D012680:Sensitivity and Specificity