title: A role for peroxisome proliferator-activated receptor gamma in resveratrol-induced colon cancer cell apoptosis.
abstract: METHODS
  Resveratrol may function as a chemopreventive agent. A recent clinical study demonstrates a reduction in tumor cell proliferation in colorectal patients receiving repeated oral ingestion of resveratrol. However, gaps remain in our knowledge of the molecular mechanisms by which resveratrol exerts its chemopreventive effect. We have previously demonstrated that resveratrol induces apoptosis in colon cancer cells and that resveratrol can sensitize chemoresistant colon cancer cells to various drugs. Based on its ability to activate peroxisome proliferator-activated receptor gamma (PPARγ) in colon cancer cells, we sought to determine the implication of this nuclear transcription factor in resveratrol-induced apoptosis.
  
  
  RESULTS
  Transient transfection of cancer cells with a dominant-negative PPARγ mutant or treatment with a PPARγ antagonist (GW9662) reversed the inhibitory effect of resveratrol. Moreover, GW9662 prevented disruption of the cell cycle induced by resveratrol and consequently abrogated resveratrol-induced apoptosis. Tumor cell death was potentiated by combining resveratrol with rosiglitazone, a PPARγ agonist.
  
  
  CONCLUSIONS
  The results show that PPARγ plays a role in resveratrol-induced apoptosis of colon carcinoma cells. The combination of resveratrol with a PPARγ agonist could be a promising pharmacological approach for treatment of colorectal cancer.
authors: Université de Bourgogne, Dijon, France; Centre de Recherche INSERM U866 - Equipe Chimiothérapie, Métabolisme Lipidique et Réponse Immunitaire Antitumorale, Dijon, France.
authors: Virginie
authors: V
authors: Aires
authors: Bertrand
authors: B
authors: Brassart
authors: Annie
authors: A
authors: Carlier
authors: Alessandra
authors: A
authors: Scagliarini
authors: Stéphane
authors: S
authors: Mandard
authors: Emeric
authors: E
authors: Limagne
authors: Eric
authors: E
authors: Solary
authors: Laurent
authors: L
authors: Martiny
authors: Michel
authors: M
authors: Tarpin
authors: Dominique
authors: D
authors: Delmas
mesh_terms: D000813:Anilides / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D047495:PPAR gamma / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000077185:Resveratrol; D000077154:Rosiglitazone; D059807:S Phase Cell Cycle Checkpoints / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D047495:PPAR gamma; D013267:Stilbenes; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D000077185:Resveratrol
keywords: Apoptosis; Cell proliferation; Colon cancer; PPAR; Polyphenols; Resveratrol