title: A copper-induced metallothionein gene from Exopalaemon carinicauda and its response to heavy metal ions.
abstract: A full-length copper-induced metallothionein (EcMT-Cu) cDNA was obtained from Exopalaemon carinicauda (Holthuis) and it contained a 198 bp open reading frame that encoded a peptide with 65 amino acid residues. Twenty-one cysteines were found in deduced amino acid sequence and the cysteine (Cys)-rich characteristic was also reported in different types of metallothioneins from other species. EcMT-Cu mRNA expression profile showed that it is the hepatopancreas specific gene. The expression of EcMT-Cu was extremely different when shrimp were exposed to seawater containing 50 μM CuSO4 or 2.5 μM CdCl2. The expression of EcMT-Cu in shrimp was significantly up-regulated at 12 and 24 h after exposure to CuSO4, however, its expression was not induced compared to that of pretreatment (p>0.05) when shrimp were exposed to CdCl2. The transcript of EcMT-Cu was found to be extremely low at gastrula and nauplius stage and expression of EcMT-Cu could be detected from egg protozoa stage.
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China. Electronic address: zhangjiquan@qdio.ac.cn.
authors: Jiquan
authors: J
authors: Zhang
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; University of Chinese Academy of Sciences, Beijing, 100039 China.
authors: Jing
authors: J
authors: Wang
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; University of Chinese Academy of Sciences, Beijing, 100039 China.
authors: Tianshu
authors: T
authors: Gui
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
authors: Zheng
authors: Z
authors: Sun
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
authors: Jianhai
authors: J
authors: Xiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007477:Ions / Q000378:metabolism* / Q000494:pharmacology; D008668:Metallothionein / Q000737:chemistry / Q000235:genetics*; D019216:Metals, Heavy / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D033541:Palaemonidae / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D007477:Ions; D019216:Metals, Heavy; C007121:copper thionein; D003300:Copper; D008668:Metallothionein
keywords: Developmental stage; E. carinicauda; Metallothionein