title: Cytopathic effect of Human cosavirus (HCoSV) on primary cell cultures of human embryonic lung MRC5.
abstract: Human cosaviruses (HCoSVs) are newly discovered viruses in Picornaviridae family. Until now, most published studies reported HCoSV detection using molecular techniques and genetic characterization of the virus. Nevertheless, no laboratory has yet reported the replication of these viruses in cultured cell lines. In the present work, the propagation of HCoSV strains isolated from human fecal specimens in MRC5 cell line and their induced cytopathic effects (CPE) was studied. The first sign of virus growth was observed 24-48h after inoculation. The cells rounded up and clumped together rapidly; empty areas became visible and, on the third day of CPE, a remarkable decrease in live cells was observed. This represents the first report on in vitro model of HCoSV replication which opens up opportunities for future investigations of these new viruses.
authors: Laboratory of Clinical Virology, WHO Regional Reference Laboratory on Poliomyelitis and Measles, Institut Pasteur de Tunis, Tunisia. Electronic address: dor_rare@yahoo.fr.
authors: Dorra
authors: D
authors: Rezig
authors: Laboratory of Clinical Virology, WHO Regional Reference Laboratory on Poliomyelitis and Measles, Institut Pasteur de Tunis, Tunisia.
authors: Henda
authors: H
authors: Touzi
authors: Laboratory of Clinical Virology, WHO Regional Reference Laboratory on Poliomyelitis and Measles, Institut Pasteur de Tunis, Tunisia.
authors: Zina
authors: Z
authors: Meddeb
authors: Laboratory of Clinical Virology, WHO Regional Reference Laboratory on Poliomyelitis and Measles, Institut Pasteur de Tunis, Tunisia.
authors: Henda
authors: H
authors: Triki
mesh_terms: D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D010849:Picornaviridae / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D014776:Virus Cultivation
keywords: Cell culture; Cytopathic effect; Human cosavirus; MRC5; Picornaviridae