title: Structural insights into the stabilization of active, tetrameric DszC by its C-terminus.
abstract: Dibenzothiophene (DBT) is a typical sulfur-containing compound found in fossil fuels. This compound and its derivatives are resistant to the hydrodesulfurization method often used in industry, but they are susceptible to enzymatic desulfurization via the 4S pathway, which is a well-studied biochemical pathway consisting of four enzymes. DBT monooxygenase (DszC) from Rhodococcus erythropolis is involved in the first step of the 4S pathway. We determined the crystal structure of DszC, which reveals that, in contrast to several homologous proteins, the C-terminus (410-417) of DszC participates in the stabilization of the substrate-binding pocket. Analytical ultracentrifugation analysis and enzymatic assays confirmed that the C-terminus is important for the stabilization of the active conformation of the substrate-binding pocket and the tetrameric state. Therefore, the C-terminus of DszC plays a significant role in the catalytic activity of this enzyme.
authors: College of Life Sciences, Nankai University, Tianjin, China.
authors: Liang
authors: L
authors: Zhang
authors: Xiaolu
authors: X
authors: Duan
authors: Daming
authors: D
authors: Zhou
authors: Zhe
authors: Z
authors: Dong
authors: Kaihua
authors: K
authors: Ji
authors: Wuyi
authors: W
authors: Meng
authors: Guoqiang
authors: G
authors: Li
authors: Xin
authors: X
authors: Li
authors: Haitao
authors: H
authors: Yang
authors: Ting
authors: T
authors: Ma
authors: Zihe
authors: Z
authors: Rao
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D030562:Databases, Protein; D004795:Enzyme Stability; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012240:Rhodococcus / Q000201:enzymology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013876:Thiophenes / Q000378:metabolism*; D014461:Ultracentrifugation
chemical_list: D001426:Bacterial Proteins; D050505:Mutant Proteins; D011994:Recombinant Proteins; D013876:Thiophenes; D010088:Oxidoreductases; C090100:dibenzothiophene monooxygenase; C016366:dibenzothiophene
keywords: C-terminus; crystal structure; enzyme mechanism; monooxygenase; tetramerization