title: The effect of platelet-rich plasma on the differentiation of synovium-derived mesenchymal stem cells.
abstract: Platelet-rich plasma (PRP), the plasma portion of blood with a high platelet concentration, has been reported to be helpful in stem cell chondrogenesis due to large amount of growth factors it contains. Here, we examined the influence of PRP on the differentiation of synovium-derived stem cells (SDSCs) and also evaluated if PRP alone was sufficient to induce SDSCs differentiation. First, the cell proliferation in various differentiation media was analyzed using the MTT assay and it was significantly higher in groups cultured with media that contained PRP. Then, We performed Safranin-O staining and type I, II, and X collagen immunohistochemistry (chondrogenesis), von Kossa staining (osteogenesis), and Oil Red O staining (adipogenesis). The staining was most prominent in groups cultured with optimized differentiation media without PRP in all three lineages of differentiation. The mRNA expression levels of typical differentiation markers were also analyzed using reverse transcription quantitative polymerase chain reaction. Although, culture in optimized differentiation media increased the mRNA expression of the typical differentiation marker genes, they were significantly reduced when cultured in the media supplemented with PRP. PRP has negative effects on SDSC differentiation in all three differentiation lineages and PRP alone does not induce SDSC differentiation.
authors: Department of Orthopaedic Surgery, Hallym University Sacred Heart Hospital, Anyang, Korea.
authors: Joon Kyu
authors: JK
authors: Lee
authors: Sahnghoon
authors: S
authors: Lee
authors: Sun Ae
authors: SA
authors: Han
authors: Sang Cheol
authors: SC
authors: Seong
authors: Myung Chul
authors: MC
authors: Lee
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008875:Middle Aged; D010011:Osteocytes / Q000166:cytology* / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013583:Synovial Membrane / Q000166:cytology* / Q000502:physiology
chemical_list: D015415:Biomarkers
keywords: chondrogenesis; mesenchymal stem cell; platelet-rich plasma; synovium-derived stem cell