title: Detoxification of acid pretreated spruce hydrolysates with ferrous sulfate and hydrogen peroxide improves enzymatic hydrolysis and fermentation.
abstract: The aim of the present work was to investigate whether a detoxification method already in use during waste water treatment could be functional also for ethanol production based on lignocellulosic substrates. Chemical conditioning of spruce hydrolysate with hydrogen peroxide (H₂O₂) and ferrous sulfate (FeSO₄) was shown to be an efficient strategy to remove significant amounts of inhibitory compounds and, simultaneously, to enhance the enzymatic hydrolysis and fermentability of the substrates. Without treatment, the hydrolysates were hardly fermentable with maximum ethanol concentration below 0.4 g/l. In contrast, treatment by 2.5 mM FeSO₄ and 150 mM H₂O₂ yielded a maximum ethanol concentration of 8.3 g/l.
authors: Technical Chemistry, Department of Chemistry, Chemical-Biological Centre, Umeå University, SE-901 87 Umeå, Sweden; Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Gothenburg, Sweden.
authors: Venkata Prabhakar
authors: VP
authors: Soudham
authors: School of Engineering, University of Borås, SE-501 90 Borås, Sweden.
authors: Tomas
authors: T
authors: Brandberg
authors: Technical Chemistry, Department of Chemistry, Chemical-Biological Centre, Umeå University, SE-901 87 Umeå, Sweden; Industrial Chemistry and Reaction Engineering, Process Chemistry Centre, Department of Chemical Engineering, Åbo Akademi University, FI-20500 Åbo-Turku, Finland.
authors: Jyri-Pekka
authors: JP
authors: Mikkola
authors: Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Gothenburg, Sweden. Electronic address: christer.larsson@chalmers.se.
authors: Christer
authors: C
authors: Larsson
mesh_terms: D056804:Biofuels; D003246:Conservation of Energy Resources; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005296:Ferrous Compounds / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D028222:Picea / Q000737:chemistry*
chemical_list: D056804:Biofuels; D005296:Ferrous Compounds; C036909:lignocellulose; C020748:ferrous sulfate; D000431:Ethanol; D008031:Lignin; D006861:Hydrogen Peroxide
keywords: Detoxification; Enzymatic hydrolysis; Fenton’s reaction; Fermentation; Lignocellulose hydrolysate