title: Expression and knockdown of the PEPC1 gene affect carbon flux in the biosynthesis of triacylglycerols by the green alga Chlamydomonas reinhardtii.
abstract: The regulation of lipid biosynthesis is important in photosynthetic eukaryotic cells. This regulation is facilitated by the direct synthesis of fatty acids and triacylglycerol (TAG), and by other controls of the main carbon metabolic pathway. In this study, knockdown of the mRNA expression of the Chlamydomonas phosphoenolpyruvate carboxylase isoform 1 (CrPEPC1) gene by RNA interference increased TAG level by 20 % but decreased PEPC activities in the corresponding transgenic algae by 39-50 %. The decrease in CrPEPC1 expression increased the expression of TAG biosynthesis-related genes, such as acyl-CoA:diacylglycerol acyltransferase and phosphatidate phosphatase. Conversely, CrPEPC1 over-expression decreased TAG level by 37 % and increased PEPC activities by 157-184 %. These observations suggest that the lipid content of algal cells can be controlled by regulating the CrPEPC1 gene.
authors: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China.
authors: Xiaodong
authors: X
authors: Deng
authors: Jiajia
authors: J
authors: Cai
authors: Yajun
authors: Y
authors: Li
authors: Xiaowen
authors: X
authors: Fei
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology; D055785:Gene Knockdown Techniques / Q000379:methods*; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D034622:RNA Interference; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014280:Triglycerides; D010730:Phosphoenolpyruvate Carboxylase