title: Ratiometric imaging of pH probes.
abstract: Measurement of intracellular pH can be readily accomplished using tools and methods described in this chapter. We present a discussion of technical considerations of various ratiometric pH-sensitive probes including dyes and genetically encoded sensors. These probes can be used to measure pH across physical scales from macroscopic whole-mount tissues down to organelles and subcellular domains. We describe protocols for loading pH-sensitive probes into single cells or tissues and discuss ratiometric image acquisition and analysis.
authors: Department of Cell and Tissue Biology, University of California, San Francisco, California, USA.
authors: Bree K
authors: BK
authors: Grillo-Hill
authors: Department of Cell and Tissue Biology, University of California, San Francisco, California, USA.
authors: Bradley A
authors: BA
authors: Webb
authors: Department of Cell and Tissue Biology, University of California, San Francisco, California, USA.
authors: Diane L
authors: DL
authors: Barber
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards; D002460:Cell Line; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D012015:Reference Standards; D059010:Single-Cell Analysis
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins
keywords: Drosophila melanogaster; Genetically encoded pH biosensor; In situ imaging; Live-cell imaging; Quantitative imaging; Ratiometric imaging; Whole-mount tissue imaging; pH measurement; pH-sensitive dyes