title: Colorimetric detection of dichlorvos using polydiacetylene vesicles with acetylcholinesterase and cationic surfactants.
abstract: Widespread use of dichlorvos in agriculture has posed serious concern for food and water contamination. A new colorimetric method for the detection of dichlorvos based on polydiacethylene and acetylcholinesterase inhibition is developed. The blue-to-red color transition of poly(10,12-pentacosadynoic acid) vesicles can be induced by myristoylcholine which is enzymatically hydrolyzed by acetylcholinesterase to myristic acid and choline to prevent the color transition. In the presence of dichlorvos, the hydrolytic activity of the enzyme is inhibited that the blue-to-red color transition is restored with a linear correlation to the dichlorvos concentration. Using UV-vis absorption spectrometer, the limit of dichlorvos detection is 6.7 ppb. A naked eye detection of 50 ppb dichlorvos is achievable by using dimiristoylphosphatidylcholine to the diacetylene mixed lipid vesicles.
authors: Program of Petrochemical and Polymer Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Program of Chemistry, Faculty of Science and Technology, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280, Thailand.
authors: Rungnapa
authors: R
authors: Pimsen
authors: Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science and Nanotec-CU Center of Excellent on Food and Agriculture, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Akachai
authors: A
authors: Khumsri
authors: Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science and Nanotec-CU Center of Excellent on Food and Agriculture, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Sumrit
authors: S
authors: Wacharasindhu
authors: National Nanotechology Center, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors: Gamolwan
authors: G
authors: Tumcharern
authors: Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science and Nanotec-CU Center of Excellent on Food and Agriculture, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: mongkol.s@chula.ac.th.
authors: Mongkol
authors: M
authors: Sukwattanasinitt
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods; D002412:Cations / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000378:metabolism; D003124:Colorimetry / Q000379:methods*; D004006:Dichlorvos / Q000032:analysis* / Q000378:metabolism; D057230:Limit of Detection; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D002412:Cations; D002800:Cholinesterase Inhibitors; D000078789:Polyacetylene Polymer; D011108:Polymers; D013501:Surface-Active Agents; D053279:Polyynes; C082361:polydiacetylene; D004006:Dichlorvos; D000110:Acetylcholinesterase
keywords: Acetycholinesterase inhibitor; Colorimetric sensor; Conjugated polymer; Dichlorvos; Organophosphate; Polydiacetylene