title: Value of microarray allergen assay in the management of eosinophilic oesophagitis.
abstract: BACKGROUND
  Eosinophilic oesophagitis (EoE) is a disorder characterised by oesophageal dysfunction and, histologically, by eosinophilic inflammation. Although treatment, which includes dilatations, oral corticosteroids and restrictive diets, is often effective, choosing the foods to be eliminated from the diet is difficult.
  
  
  OBJECTIVE
  Component resolved diagnostic by microarray allergen assay may be useful in detecting allergens that might be involved in the inflammatory process.
  
  
  METHODS
  We studied 67 patients with EoE, diagnosed clinically and histologically by endoscopic biopsy. CRD analysis with microarray technology was carried out in the 67 EoE patients, 50 patients with pollen allergy without digestive symptoms, and 50 healthy controls.
  
  
  RESULTS
  Allergies were not detected by microarray in only seven of the 67 patients with EoE. Controls with pollen allergy showed sensitisation to different groups of pollen proteins without significant differences. In EoE patients with response to some allergens, the predominant allergens were grasses group 1 and, in particular, nCyn d 1 (Cynodon dactylon) or Bermuda grass pollen in 59.5%, followed by lipid transfer proteins (LTP) of peach (19.40%), hazelnut (17.91%) and Artemisia (19.40%).
  
  
  CONCLUSIONS
  In patients with EoE, sensitisation to plant foods and pollen is important. The proteins most frequently involved are nCyn d 1 and lipid transfer proteins, hazelnuts and walnuts. After one year of an array-guided exclusion diet and pollen-specific immunotherapy in the case of high levels of response, patients with EoE showed preliminary significant improvements.
authors: Allergy Unit, University Hospital Rio Hortega, Valladolid, Spain. Electronic address: aliciaarmentia@gmail.com.
authors: A
authors: A
authors: Armentia
authors: Paediatric Service, Hospital Universitario Río Hortega, Valladolid, Spain.
authors: S
authors: S
authors: Martín
authors: Gastroenterology Service, Hospital Universitario Río Hortega, Valladolid, Spain.
authors: J
authors: J
authors: Barrio
authors: Allergy Unit, University Hospital Rio Hortega, Valladolid, Spain.
authors: B
authors: B
authors: Martín
authors: Allergy Unit, University Hospital Rio Hortega, Valladolid, Spain.
authors: J C
authors: JC
authors: García
authors: Allergy Unit, University Hospital Rio Hortega, Valladolid, Spain.
authors: J M
authors: JM
authors: Vega
authors: Allergy Unit, University Hospital Rio Hortega, Valladolid, Spain.
authors: A
authors: A
authors: Sánchez
authors: Gastroenterology Service, Hospital Universitario Río Hortega, Valladolid, Spain.
authors: P
authors: P
authors: Fernández
authors: Immunology Department, University of Valladolid, School of Medicine, Valladolid, Spain.
authors: A
authors: A
authors: Corell
mesh_terms: D000293:Adolescent; D000328:Adult; D052179:Antigens, Plant / Q000276:immunology; D019306:Artemisia / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology; D031211:Corylus / Q000276:immunology; D031744:Cynodon / Q000276:immunology; D003888:Desensitization, Immunologic; D057765:Eosinophilic Esophagitis / Q000175:diagnosis* / Q000178:diet therapy; D005247:Feeding Behavior; D005260:Female; D006801:Humans; D008297:Male; D046228:Microarray Analysis / Q000379:methods*; D010940:Plant Proteins / Q000276:immunology; D011058:Pollen / Q000276:immunology; D027861:Prunus / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis* / Q000276:immunology / Q000628:therapy*; D055815:Young Adult
chemical_list: D052179:Antigens, Plant; D002352:Carrier Proteins; C550205:Cyn d 1 allergen, Bermuda grass; D010940:Plant Proteins; C511920:lipid transfer proteins, plant
keywords: Eosinophilic oesophagitis; Microarrays; Molecular analysis