title: [Giardia duodenalis genotypes found in the Instituto Colombiano de Bienestar Familiar day care centers and dogs in Ibagué, Colombia].
abstract: BACKGROUND
  Eight Giardia duodenalis genotypes (A-H) have been described to date. Genotypes A and B have been isolated from humans and a wide range of mammals; however, genotypes C-H have shown greater host specificity.
  
  
  OBJECTIVE
  Identifying G. duodenalis genotypes from cysts in faeces obtained from children attending the Instituto Colombiano de Bienestar Familiar (ICBF) day care centres and from dogs in Ibagué by PCR-RFLP targeting both the b -giardin and glutamate dehydrogenase genes.
  
  
  METHODS
  Cysts from G. duodenalis positive samples were concentrated, DNA was extracted and the b -giardin and glutamate dehydrogenase genes were analysed by PCR-RFLP. The MHOM/CO/04/G40 strain was used as positive control (this was obtained from the Grupo de Parasitología at the Instituto Nacional de Salud ).
  
  
  RESULTS
  Of the total human samples, 11/23 (48%) were genotyped as A and 12/23 (52%) as B; PCR-RFLP revealed that four canine samples were genotypes C and D, these being host-specific.
  
  
  CONCLUSIONS
  Only genotypes associated with human infection (AII, BIII and BIV) were found in the children and host-specific genotypes were observed in canines (C and D). No interaction could be established between animal and human transmission cycles due to the small canine sample size and as the former did not come into contact with children attending ICBF day-care centres.
authors: Facultad de Medicina Veterinaria y Zootecnia, Universidad del Tolima, Ibagué, Colombia.
authors: Victoria
authors: V
authors: Rodríguez
authors: Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Departamento de Biología, Universidad del Tolima, Ibagué, Colombia.
authors: Oneida
authors: O
authors: Espinosa
authors: Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Departamento de Biología, Universidad del Tolima, Ibagué, Colombia.
authors: Julio César
authors: JC
authors: Carranza
authors: Grupo de Parasitología, Subdirección de Investigación Científica y Tecnológica, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C, Colombia.
authors: Sofía
authors: S
authors: Duque
authors: Grupo de Parasitología, Subdirección de Investigación Científica y Tecnológica, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C, Colombia.
authors: Adriana
authors: A
authors: Arévalo
authors: Departamento de Matemáticas y Estadística, Facultad de Ciencias, Universidad del Tolima, Ibagué, Colombia.
authors: Jairo Alfonso
authors: JA
authors: Clavijo
authors: Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Departamento de Biología, Universidad del Tolima, Ibagué, Colombia.
authors: Daniel Alfonso
authors: DA
authors: Urrea
authors: Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Departamento de Biología, Universidad del Tolima, Ibagué, Colombia.
authors: Gustavo Adolfo
authors: GA
authors: Vallejo
mesh_terms: D000328:Adult; D000818:Animals; D002656:Child Day Care Centers; D002675:Child, Preschool; D003105:Colombia / Q000453:epidemiology; D003598:Cytoskeletal Proteins / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D005260:Female; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology* / Q000662:veterinary; D005969:Glutamate Dehydrogenase / Q000235:genetics; D006801:Humans; D007223:Infant; D008297:Male; D033661:Oocysts; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics; D015047:Zoonoses
chemical_list: D003598:Cytoskeletal Proteins; D015800:Protozoan Proteins; C038207:giardin protein, Giardia lamblia; D005969:Glutamate Dehydrogenase