title: Proteomic analyses of the time course responses of mice infected with Brucella abortus 544 reveal immunogenic antigens.
abstract: Brucellosis is a major zoonotic disease caused by pathogens of the genus Brucella. The eradication of brucellosis in domestic animals, associated with the prevention of human infection, can be attained through accurate diagnosis. However, the conventional serological diagnosis of brucellosis has limitations, particularly in detecting the infection period. Accordingly, the aim of this study was to determine reliable immunogenic proteins to detect Brucella abortus infection according to time course responses to aid in the appropriate management of this disease. Proteomic identification through two-dimensional electrophoresis (2DE), followed by immunoblotting, revealed 13, 24, and 55 immunodominant B. abortus 544 proteins that were reactive to sera from experimentally infected mice at early (10 days), middle (30 days), and late (60 days) infection periods, respectively. After excluding several spots reactive to sera from Yersinia enterocolitica O:9-infected and noninfected mice, 17 of the 67 immunodominant proteins were identified through MALDI-TOF MS. Consequently, the identified proteins showed time course-dependent immunogenicity against Brucella infection. Thus, the results of this study suggest that the production of immunogenic proteins during infection periods improves the diagnosis and discovery of vaccine candidates.
authors: Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Korea.
authors: Jin Ju
authors: JJ
authors: Lee
authors: Hannah Leah
authors: HL
authors: Simborio
authors: Alisha Wehdnesday Bernardo
authors: AW
authors: Reyes
authors: Dae Geun
authors: DG
authors: Kim
authors: Huynh Tan
authors: HT
authors: Hop
authors: Wongi
authors: W
authors: Min
authors: Moon
authors: M
authors: Her
authors: Suk Chan
authors: SC
authors: Jung
authors: Han Sang
authors: HS
authors: Yoo
authors: Suk
authors: S
authors: Kim
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology*; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015151:Immunoblotting; D008807:Mice, Inbred BALB C; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D015008:Yersinia enterocolitica
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D020543:Proteome
keywords: Brucella abortus; immunogenic proteins; infection period; serodiagnosis