title: Combination of a novel photosensitizer DTPP with 650 nm laser results in efficient apoptosis, arresting cell cycle and cytoskeleton protein changes in lung cancer A549 cells.
abstract: Photodynamic therapy (PDT) using photosensitized reaction to produce cytotoxicity was used for cancer therapy in recent years. To study the effectiveness of PDT mediated by a novel photosensitizer (PS), DTPP 5-(4'-(2″-dicarboxymethylamino)acetamidophenyl)-10, 15, 20-triphenylporphyrin, on lung cancer A549 cell lines in vitro, DTPP was employed in different concentrations (2, 4, 6, 8, 10, 12, 15, 20, 25, and 30 μg/ml) and combined with 650 nm laser of different power densities (0.6, 1.2, 2.4, 4.8, 7.2, and 9.6 J/cm(2)) that resulted in obvious inhibition of cell proliferation and apoptosis. Results showed that cell survival rates have a dependent relationship with time and PS concentrations and no significant cytotoxicity was induced by DTPP itself. Apoptosis and cell cycle S arrest were observed; cytoskeleton morphologic observation revealed collapse, sparkling, and shrunken shapes. Apoptosis-related protein caspase-3 overexpression was detected while caspase-9, bcl-2, and cytoskeleton protein beta-catenin were in low levels of expression than the control. Cleavage of beta-catenin by caspase-3 or other proteases from the lysosome might be the main reason for the cytoskeleton collapse as beta-tubulin and actin were at a stable level 12 h after PDT. This paper gives a better understanding of the effectiveness of DTPP-mediated PDT in lung cancer A549 cells both with regard to dosimetry and apoptosis changes.
authors: Laboratory of Laser Medicine, Institute of Biomedical Engineering, Academy of Medical Science and Peking Union Medical College, Tianjin, 300192, China, dr.wangh@outlook.com.
authors: H
authors: H
authors: Wang
authors: H M
authors: HM
authors: Zhang
authors: H J
authors: HJ
authors: Yin
authors: M Q
authors: MQ
authors: Wei
authors: H
authors: H
authors: Sha
authors: T J
authors: TJ
authors: Liu
authors: Y X
authors: YX
authors: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects / Q000528:radiation effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007834:Lasers; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy*; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000627:therapeutic use*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology / Q000627:therapeutic use*; D016196:S Phase / Q000187:drug effects / Q000528:radiation effects
chemical_list: D003598:Cytoskeletal Proteins; D009943:Organophosphorus Compounds; D017319:Photosensitizing Agents; C014475:DTPP; D053148:Caspase 3