title: Determination of starch lysophospholipids in rice using liquid chromatography-mass spectrometry (LC-MS).
abstract: Acquiring a complete understanding of rice starch lysophospholipids (LPLs), their biosynthetic pathways and genetic diversity, and the influence of genotype by environment interactions has been hampered by the lack of efficient high-throughput extraction and analysis methods. It was hypothesized a single-step aqueous n-propanol extraction combined with liquid chromatography-mass spectrometry (LC-MS) could be employed to analyze starch LPLs in white rice. The investigation found different grinding methods showed little effect on the final LPL detected, and a simple single-step extraction with 75% n-propanol (8 mL/0.15 g) heated at 100 °C for 2 h was as effective as an onerous multistep extraction method. A LC-MS method was optimized to simultaneously quantify 10 major LPLs in rice starch within 15 min. This method enables total and individual starch LPL analysis of a large number of rice samples at little cost. This approach could be applied to starch LPLs in other cereals.
authors: Southern Cross Plant Science, Southern Cross University , Lismore, NSW 2480, Australia.
authors: Lei
authors: L
authors: Liu
authors: Chuan
authors: C
authors: Tong
authors: Jinsong
authors: J
authors: Bao
authors: Daniel L E
authors: DL
authors: Waters
authors: Terry J
authors: TJ
authors: Rose
authors: Graham J
authors: GJ
authors: King
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D008246:Lysophospholipids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D012275:Oryza / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013213:Starch / Q000737:chemistry*
chemical_list: D008246:Lysophospholipids; D013213:Starch