title: Comparative Analysis of Human Epidermal and Peripheral Blood γδ T Cell Cytokine Profiles.
abstract: BACKGROUND
  Human epidermal γδ T cells are known to play crucial roles in the defense and homeostasis of the skin. However, their precise mechanism of action in skin inflammation remains less clear.
  
  
  OBJECTIVE
  In this study, we analyzed the cytokine expression profile of human epidermal γδ T cells and compared it to that of peripheral blood γδ T cells to investigate the specific activity of epidermal γδ T cells in modulating skin inflammation.
  
  
  METHODS
  We isolated γδ T cells from epidermal tissue or peripheral blood obtained from healthy volunteers. Isolated γδ T cells were stimulated using immobilized anti-CD3 antibody and interleukin-2 plus phytohaemagglutinin, and were then analyzed using a cytokine array kit.
  
  
  RESULTS
  Both epidermal and peripheral blood γδ T cells produced comparable levels of granulocyte-macrophage colony-stimulating factor, I-309, interferon-γ, macrophage migration inhibitory factor, macrophage inflammatory protein-1α, and chemokine (C-C) ligand 5. The epidermal γδ T cells produced significantly higher levels of interleukin-4, -8, -13, and macrophage inflammatory protein-1β than the peripheral blood γδ T cells did. Notably, the epidermal γδ T cells produced several hundred-fold higher levels of interleukin-13 than interleukin-4.
  
  
  CONCLUSIONS
  These results suggest that the epidermal γδ T cells have a stronger potential to participate in the Th2-type response than the peripheral blood γδ T cells do. Furthermore, epidermal γδ T cells might play an important role in the pathogenesis of Th2-dominant skin diseases because of their active production of interleukin-13.
authors: Laboratory of Immunology, College of Pharmacy, Dankook University, Cheonan, Korea.
authors: Kwangmi
authors: K
authors: Kim
authors: Bioscience Research Institute, Amorepacific Corporation R&D Center, Yongin, Korea.
authors: Jiyeon
authors: J
authors: Han
authors: Bioscience Research Institute, Amorepacific Corporation R&D Center, Yongin, Korea.
authors: Tae Ryong
authors: TR
authors: Lee
authors: Bioscience Research Institute, Amorepacific Corporation R&D Center, Yongin, Korea.
authors: Dong Wook
authors: DW
authors: Shin
authors: Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul, Korea. ; Institute of Human-Environment Interface Biology, Medical Research Center, Seoul National University, Seoul, Korea.
authors: Hak
authors: H
authors: Chang
authors: Institute of Human-Environment Interface Biology, Medical Research Center, Seoul National University, Seoul, Korea. ; Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea.
authors: A-Ri
authors: AR
authors: Cho
authors: Institute of Human-Environment Interface Biology, Medical Research Center, Seoul National University, Seoul, Korea. ; Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea.
authors: Soon Jin
authors: SJ
authors: Choi
authors: Institute of Human-Environment Interface Biology, Medical Research Center, Seoul National University, Seoul, Korea. ; Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea.
authors: Seong Jin
authors: SJ
authors: Jo
authors: Institute of Human-Environment Interface Biology, Medical Research Center, Seoul National University, Seoul, Korea. ; Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea.
authors: Ohsang
authors: O
authors: Kwon
keywords: Epidermis; Interleukin-13; Interleukin-4; γδ T cells