title: Molecular identification of necrophagous muscidae and sarcophagidae fly species collected in Korea by mitochondrial cytochrome C oxidase subunit I nucleotide sequences.
abstract: Identification of insect species is an important task in forensic entomology. For more convenient species identification, the nucleotide sequences of cytochrome c oxidase subunit I (COI) gene have been widely utilized. We analyzed full-length COI nucleotide sequences of 10 Muscidae and 6 Sarcophagidae fly species collected in Korea. After DNA extraction from collected flies, PCR amplification and automatic sequencing of the whole COI sequence were performed. Obtained sequences were analyzed for a phylogenetic tree and a distance matrix. Our data showed very low intraspecific sequence distances and species-level monophylies. However, sequence comparison with previously reported sequences revealed a few inconsistencies or paraphylies requiring further investigation. To the best of our knowledge, this study is the first report of COI nucleotide sequences from Hydrotaea occulta, Muscina angustifrons, Muscina pascuorum, Ophyra leucostoma, Sarcophaga haemorrhoidalis, Sarcophaga harpax, and Phaonia aureola.
authors: Department of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of Korea ; DaejeonDaejeon Institute of National Forensic Service, 1524 Yuseongdae-ro, Yuseong-gu, Daejeon 305-348, Republic of Korea.
authors: Yu-Hoon
authors: 0000-0001-7291-132X
authors: YH
authors: Kim
authors: Department of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of Korea.
authors: Sang Eon
authors: 0000-0003-4165-8119
authors: SE
authors: Shin
authors: Department of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of Korea.
authors: Chan Seon
authors: CS
authors: Ham
authors: Department of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of Korea.
authors: Seong Yoon
authors: SY
authors: Kim
authors: Department of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of Korea.
authors: Kwang Soo
authors: KS
authors: Ko
authors: Department of Science Education, Chinju National University of Education, 3 Jinyangho-ro 369 gil, Jinju 660-756, Republic of Korea.
authors: Tae-Ho
authors: TH
authors: Jo
authors: Department of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of Korea.
authors: Gi Hoon
authors: GH
authors: Son
authors: Department of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of Korea.
authors: Seong Hwan
authors: 0000-0003-2096-2664
authors: SH
authors: Park
authors: Department of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of Korea.
authors: Juck-Joon
authors: 0000-0002-2207-4233
authors: JJ
authors: Hwang
mesh_terms: D000818:Animals; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D009117:Muscidae / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D056910:Republic of Korea; D058493:Sarcophagidae / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D003576:Electron Transport Complex IV