title: Genomic mapping of cAMP receptor protein (CRP Mt) in Mycobacterium tuberculosis: relation to transcriptional start sites and the role of CRPMt as a transcription factor.
abstract: Chromatin immunoprecipitation identified 191 binding sites of Mycobacterium tuberculosis cAMP receptor protein (CRP(Mt)) at endogenous expression levels using a specific α-CRP(Mt) antibody. Under these native conditions an equal distribution between intragenic and intergenic locations was observed. CRP(Mt) binding overlapped a palindromic consensus sequence. Analysis by RNA sequencing revealed widespread changes in transcriptional profile in a mutant strain lacking CRP(Mt) during exponential growth, and in response to nutrient starvation. Differential expression of genes with a CRP(Mt)-binding site represented only a minor portion of this transcriptional reprogramming with ∼ 19% of those representing transcriptional regulators potentially controlled by CRP(Mt). The subset of genes that are differentially expressed in the deletion mutant under both culture conditions conformed to a pattern resembling canonical CRP regulation in Escherichia coli, with binding close to the transcriptional start site associated with repression and upstream binding with activation. CRP(Mt) can function as a classical transcription factor in M. tuberculosis, though this occurs at only a subset of CRP(Mt)-binding sites.
authors: Division of Mycobacterial Research, MRC National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK.
authors: Christina
authors: C
authors: Kahramanoglou
authors: Division of Mycobacterial Research, MRC National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK tcortes@nimr.mrc.ac.uk.
authors: Teresa
authors: T
authors: Cortes
authors: Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India.
authors: Nishad
authors: N
authors: Matange
authors: Division of Mycobacterial Research, MRC National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK.
authors: Debbie M
authors: DM
authors: Hunt
authors: Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India.
authors: Sandhya S
authors: SS
authors: Visweswariah
authors: Division of Mycobacterial Research, MRC National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK Centre for Molecular Bacteriology and Infection, Imperial College London, SW7 2AZ, UK.
authors: Douglas B
authors: DB
authors: Young
authors: Division of Mycobacterial Research, MRC National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK.
authors: Roger S
authors: RS
authors: Buxton
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D002874:Chromosome Mapping; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein