title: Donor verification using Short Tandem Repeat (STR) analysis directly from blood collected in PAXgene RNA tubes.
abstract: Biorepository processing includes nucleic acid extractions in batch mode from a large number of blood samples from many different donors. Handling such a large number of biospecimens presents the challenge of ensuring that samples are not switched or mislabeled during processing. One approach for confirming donor identity from DNA samples is the use of multiplexed fluorescent PCR for detecting Short Tandem Repeat (STR) allelic-size polymorphisms for a set of common autosomal loci. While donor identity of DNA extracted directly from blood collected in standard tubes containing anticoagulants can be easily verified by generating STR profiles, RNA from blood collected in PAXgene Blood RNA tubes (PAXgene RNA tubes) is depleted of DNA and is not amenable to STR fingerprinting for donor identity verification. We investigated the feasibility of isolating DNA directly from blood collected in PAXgene RNA tubes for use as template for STR DNA fingerprinting for blood donor identity verification. We determined that DNA extraction can be performed manually with the QIAamp DNA Blood Minikit or on the QIAxtractor instrument with minimal pre-processing protocol additions, and that DNA isolated from blood collected in PAXgene RNA tubes is of sufficient quantity and quality for successful STR fingerprint analysis. Adaptation of quality assurance methods such as the PAXgene RNA tube DNA extraction/STR fingerprinting assay described here is a good practice that ensures that biobanking collections provide scientists with high quality, donor-verified biomaterial.
authors: Molecular Biology Group, Coriell Institute for Medical Research , Camden, New Jersey.
authors: Victoria R
authors: VR
authors: Kelly
authors: Susan P
authors: SP
authors: Jones
authors: Holly L
authors: HL
authors: Sammartino
authors: Dennis Ian S
authors: DI
authors: Arocena
authors: Steven J
authors: SJ
authors: Madore
mesh_terms: D018070:Biological Specimen Banks; D001782:Blood Donors; D001800:Blood Specimen Collection / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000097:blood / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D006801:Humans; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction
chemical_list: D004247:DNA