title: Fast multipoint immobilized MOF bioreactor.
abstract: An enzyme-NBD@MOF bioreactor with exemplary proteolytic performance, even after successive reuse and storage, was produced through a novel, rapid and simple multipoint immobilization technique without chemical modification of the solid support. Enzyme loading and distribution could be directly monitored from the fluorescence emission of the bioreactor. The dye molecular dimension plays a role in its overall performance.
authors: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li, 320 (Taiwan).
authors: Wan-Ling
authors: WL
authors: Liu
authors: Cheng-You
authors: CY
authors: Wu
authors: Chien-Yu
authors: CY
authors: Chen
authors: Brenda
authors: B
authors: Singco
authors: Chia-Her
authors: CH
authors: Lin
authors: Hsi-Ya
authors: HY
authors: Huang
mesh_terms: D000818:Animals; D019149:Bioreactors; D002417:Cattle; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D007700:Kinetics; D009942:Organometallic Compounds / Q000737:chemistry*; D059748:Proteolysis; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014357:Trypsin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D012710:Serum Albumin, Bovine; D014357:Trypsin
keywords: biocatalysis; bioreactors; enzymes; immobilization; metal-organic frameworks