title: A simple agar plate preparation for effective transfer of Ureaplasma colonies onto nitrocellulose membranes for colony immunoblotting.
abstract: A simple method for preparing agar plates is presented, which allows an efficient transfer of Ureaplasma colonies to nitrocellulose membranes for subsequent immunological detection. This simple and reproducible procedure was used to demonstrate antigenic variation in the phase-variable mba-locus of Ureaplasma parvum serovar 3.
authors: Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Austria. Electronic address: carl-ulrich.zimmerman@vetmeduni.ac.at.
authors: Carl-Ulrich R
authors: CU
authors: Zimmerman
authors: Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Austria.
authors: Thomas
authors: T
authors: Stiedl
authors: Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Austria.
authors: Joachim
authors: J
authors: Spergser
authors: Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Austria.
authors: Renate
authors: R
authors: Rosengarten
mesh_terms: D000940:Antigenic Variation; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology; D015151:Immunoblotting / Q000295:instrumentation / Q000379:methods*; D014509:Ureaplasma / Q000737:chemistry* / Q000254:growth & development / Q000276:immunology
chemical_list: D001426:Bacterial Proteins
keywords: Colony immunoblot; Colony morphology; Colony size; Hemadsorption; Multiple banded antigen; Ureaplasma