title: Inhibitory effect of fucosylated chondroitin sulfate from the sea cucumber Acaudina molpadioides on adipogenesis is dependent on Wnt/β-catenin pathway.
abstract: The fucosylated chondroitin sulfate was isolated from the sea cucumber Acaudina molpadioides (Am-CHS) and its effect on the adipogenesis was investigated in vitro. Results showed that Am-CHS exhibited a marked anti-adipogenic effect. The TG contents of cells treated with Am-CHS from the early stage, mid stage and later stage were decreased by 31.76%, 19.76% and 13.85%, respectively. Wnt/β-catenin pathway acts as a negative regulator of adipogenesis. It is showed that Am-CHS enhanced the expression of the Wnt/β-catenin related factors, namely Wnt10b, β-catenin, Fz and LRP, causing more cytoplasmic β-catenin translocated into the nuclear to inhibit the expression of PPARγ and C/EBPα. SREBP-1c promotes the adipocyte differentiation by enhancing the activity of PPARγ. Reverse transcriptase-polymerase chain reaction assay and western blotting results revealed that the expressions of SREBP-1c and its transcriptional product FAS were inhibited by Am-CHS. Further study showed that Am-CHS also suppressed the PPARγ and C/EBPα expression. These results indicate that the anti-adipogenic activity of Am-CHS may exhibited by activating the Wnt/β-catenin pathway and down-regulating the SREBP-1c expression. To further verify this conclusion, in vivo experiments were performed. Results revealed that Am-CHS reduced the body adipose rate compared to high-fat-high sucrose diet (HFSD)-fed mice. Western blotting results showed that Am-CHS increased the expression of β-catenin, a negative regulator in adipogenesis, and decreased that of SREBP-1c, PPARγ and C/EBPα, the positive regulators of adipogenesis in the adipose tissue compared to the HFSD-fed group. These results further confirmed the anti-adipogenic activity of Am-CHS.
authors: College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, China.
authors: Hui
authors: H
authors: Xu
authors: College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, China.
authors: Jingfeng
authors: J
authors: Wang
authors: College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, China.
authors: Xin
authors: X
authors: Zhang
authors: College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, China.
authors: Zhaojie
authors: Z
authors: Li
authors: College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, China.
authors: Yuming
authors: Y
authors: Wang
authors: College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, China. Electronic address: xuech@ouc.edu.cn.
authors: Changhu
authors: C
authors: Xue
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D053222:Fas Ligand Protein / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D047495:PPAR gamma / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012616:Sea Cucumbers / Q000737:chemistry*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics; D051153:Wnt Proteins / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D053222:Fas Ligand Protein; D047495:PPAR gamma; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D051153:Wnt Proteins; D051176:beta Catenin; C517150:fucosylated chondroitin sulfate; D002809:Chondroitin Sulfates
keywords: Adipogenesis; CCAAT/enhancer binding protein-α (C/EBPα); Fucosylated chondroitin sulfate; Peroxisome proliferator-activated receptor-γ (PPARγ); Sterol regulatory element-binding protein (SREBP-1c); Wnt/β-catenin pathway