title: Hydrogen-rich water reestablishes ROS homeostasis but exerts differential effects on anthocyanin synthesis in two varieties of radish sprouts under UV-A irradiation.
abstract: The aims of the study were to investigate whether hydrogen gas (H2) was involved in regulation of anthocyanin biosynthesis in two contrasting radish (Raphanus sativus L.) varieties (low [LA] and high [HA] level of anthocyanin) under UV irradiation. The results showed that hydrogen-rich water (HRW) significantly blocked the UV-A-induced increase of H2O2 and O2(•-) accumulation, and enhanced the UV-A-induced increase of superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities in LA and HA. Furthermore, UV-A-induced increase of anthocyanin and total phenols was further enhanced only in HA sprouts cotreated with HRW. LC-MS/MS analysis showed that five anthocyanidins existed in HA sprouts, but only two in LA sprouts. Meanwhile, the cyanidin was the most abundant anthocyanidin in HA, and the cyanidin was 2-fold higher cotreated with HRW than UV-A. Molecular analyses showed that the anthocyanin biosynthesis-related genes were upregulated significantly in both HA (in particular) and LA sprouts treated with HRW plus UV-A. These data imply that HRW reestablishes reactive oxygen species homeostasis in both LA and HA, but exerts different effects on anthocyanin accumulation between them under UV-A.
authors: College of Life Sciences, Nanjing Agricultural University , Nanjing 210095, Jiangsu, P. R. China.
authors: Nana
authors: N
authors: Su
authors: Qi
authors: Q
authors: Wu
authors: Yuanyuan
authors: Y
authors: Liu
authors: Jiangtao
authors: J
authors: Cai
authors: Wenbiao
authors: W
authors: Shen
authors: Kai
authors: K
authors: Xia
authors: Jin
authors: J
authors: Cui
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D006706:Homeostasis; D006859:Hydrogen / Q000032:analysis / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism; D031224:Raphanus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays; D014867:Water / Q000032:analysis
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014867:Water; D006859:Hydrogen; D060387:Ascorbate Peroxidases; D013482:Superoxide Dismutase