title: Selective binding of single-stranded DNA-binding proteins onto DNA molecules adsorbed on single-walled carbon nanotubes.
abstract: Single-stranded DNA-binding (SSB) proteins were treated with hybrids of DNA and single-walled carbon nanotubes (SWNTs) to examine the biological function of the DNA molecules adsorbed on the SWNT surface. When single-stranded DNA (ssDNA) was used for the hybridization, significant binding of the SSB molecules to the ssDNA-SWNT hybrids was observed by using atomic force microscopy (AFM) and agarose gel electrophoresis. When double-stranded DNA (dsDNA) was used, the SSB molecules did not bind to the dsDNA-SWNT hybrids in most of the conditions that we evaluated. A specifically modified electrophoresis procedure was used to monitor the locations of the DNA, SSB, and SWNT molecules. Our results clearly showed that ssDNA/dsDNA molecules on the SWNT surfaces retained their single-stranded/double-stranded structures.
authors: Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan.
authors: Daisuke
authors: D
authors: Nii
authors: Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan.
authors: Takuya
authors: T
authors: Hayashida
authors: Cellular and Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan; Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors: Yuuki
authors: Y
authors: Yamaguchi
authors: Cellular and Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors: Shukuko
authors: S
authors: Ikawa
authors: Cellular and Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors: Takehiko
authors: T
authors: Shibata
authors: Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan. Electronic address: meicun2006@163.com.
authors: Kazuo
authors: K
authors: Umemura
mesh_terms: D000327:Adsorption; D000818:Animals; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D018625:Microscopy, Atomic Force; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D012474:Salmon
chemical_list: D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D037742:Nanotubes, Carbon
keywords: Agarose gel electrophoresis; Atomic force microscopy; Carbon nanotube; Molecular recognition; Single-stranded DNA binding protein