title: Inhibitory effect of allyl isothiocyanate on platelet aggregation.
abstract: Allyl isothiocyanate (AITC) is one of the major components of mustard. The present study for the first time attempted to evaluate the effect of AITC on platelet aggregation. In the in vitro study using platelet-rich plasma (PRP) from rats and humans, AITC at concentrations of 100 and 300 μM significantly inhibited platelet aggregation induced by collagen, thrombin, ADP, and arachidonic acid. AITC also attenuated thromboxane A2 production and ATP release in rat and human PRP. AITC elicited inhibitory effects on cellular Ca(2+) increase and platelet shape change in rat PRP. AITC further showed inhibitory effects on the phosphorylation of PKCδ, p38, ERK, and Akt in rat PRP. In the rat ex vivo study, 1 and 3 mg/kg (po) of AITC showed significant inhibitory effect on platelet aggregation. Furthermore, AITC showed a protective effect in thromboembolism attack model in mouse. These results suggest that AITC has remarkable antiplatelet effects and maybe a therapeutic potential for the prevention of aberrant platelet activation-related disorders.
authors: College of Pharmacy and ‡College of Pharmacy, Research Institute of Pharmaceutical Sciences and Technology, Ajou University , Suwon 443-749, Republic of Korea.
authors: Do-Seop
authors: DS
authors: Lee
authors: Tae-Ho
authors: TH
authors: Kim
authors: Yi-Sook
authors: YS
authors: Jung
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D010766:Phosphorylation; D010974:Platelet Aggregation / Q000187:drug effects*; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013928:Thromboxane A2 / Q000096:biosynthesis
chemical_list: D017879:Isothiocyanates; D013928:Thromboxane A2; D000255:Adenosine Triphosphate; C004471:allyl isothiocyanate; D011494:Protein Kinases; D002118:Calcium