title: Validation of an LC-MS/MS method for the quantitative determination of a novel fungicide: pyraoxystrobin in rat plasma and its application to a toxicokinetics study.
abstract: A simple, sensitive, and reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed for determination of pyraoxystrobin in rat plasma and applied to a toxicokinetics study. The separation was performed by gradient elution on a Luna 5 μ C18 (2) 100 Å column (50 × 4.6 mm I.D., 5 μm) with mobile phase: water (0.1 % formic acid, v/v)/acetonitrile (0.1 % formic acid, v/v), followed by quantification with a mass detector in multiple reaction monitoring (MRM) mode using ESI as an interface. The calibration curve was linear over a concentration range of 1.00-200 ng/mL. The recovery for pyraoxystrobin ranged from 101.4 to 108.2 %. The intraday bias and precision ranged from -9.3 to 8.1 % and from 0.7 to 8.4 %, respectively, and the interday bias and precision ranged from -0.3 to 4.0 % and from 4.4 to 7.2 %, respectively. The toxicokinetics of pyraoxystrobin after single 100 and 1,000 mg/kg oral doses were studied in rats.
authors: Department of Analytical Chemistry, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China, linlihong@sinochem.com.
authors: Lihong
authors: L
authors: Lin
authors: Wei
authors: W
authors: Yu
authors: Yanan
authors: Y
authors: Pang
authors: Changsong
authors: C
authors: Sun
authors: Qinghe
authors: Q
authors: Meng
authors: Xiaolei
authors: X
authors: Li
authors: Xingjie
authors: X
authors: Guo
mesh_terms: D000179:Acrylates / Q000097:blood* / Q000737:chemistry; D000284:Administration, Oral; D000818:Animals; D000935:Antifungal Agents / Q000097:blood* / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D011720:Pyrazoles / Q000097:blood* / Q000737:chemistry; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D066007:Toxicokinetics
chemical_list: C000590225:2-(2-((3-(4-chlorophenyl)-1-methyl-1H-pyrazol-5-yloxy)methyl)phenyl)-3-methoxyacrylate; D000179:Acrylates; D000935:Antifungal Agents; D011720:Pyrazoles