title: Copy number changes of 4-gene set may predict early relapse in advanced epithelial ovarian cancer after initial platinum-paclitaxel chemotherapy.
abstract: For advanced epithelial ovarian cancer (EOC), time to recurrence (TTR) is an important indicator to gauge the therapeutic efficacy of postoperative adjuvant chemotherapy. Our objective was to determine the genes that could potentially distinguish patients with short versus long TTR after initial administration of platinum-paclitaxel combination chemotherapy in advanced EOC. Tumor samples of 159 patients were obtained during the primary cytoreduction. Array comparative genomic hybridization (CGH) was carried with genomic DNA from 17 EOC samples (8 with TTR > 15 months and 9 with TTR ≤ 6 months) to screen candidate gene set, copy-number changes (CNC) of which were significantly different between early and late relapse cases. Seventeen candidate genes were identified by array CGH. The analysis of consistency between real-time PCR and array CGH revealed that 4 genes displayed consistent results, namely GSTT1, ISG20L1, STARD5 and FREM1. In a 142-case validation set, CNC of 4 candidate genes was evaluated and verified by real-time PCR. Sixty five point five percent of the patients were correctly divided into early (TTR ≤ 10 months) and late (TTR > 10 months) recurrent group by CNC of the 4 genes using discriminant analysis. The results showed that CNC of 4-gene set could potentially determine early (TTR ≤ 10 months) or late relapse (TTR > 10 months) after initial platinum-paclitaxel combination chemotherapy in advanced EOC.
authors: Department of Gynecological Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Ning
authors: N
authors: Li
authors: Department of Gynecological Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Jin-Lin
authors: JL
authors: Hou
authors: State Key Laboratory of Molecular Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Zhi-Zhou
authors: ZZ
authors: Shi
authors: Department of Gynecological Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Xiao-Guang
authors: XG
authors: Li
authors: Department of Gynecological Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Nan
authors: N
authors: Li
authors: Department of Gynecological Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Yang-Chun
authors: YC
authors: Sun
authors: State Key Laboratory of Molecular Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Xin
authors: X
authors: Xu
authors: State Key Laboratory of Molecular Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Yan
authors: Y
authors: Cai
authors: Department of Pathology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Xun
authors: X
authors: Zhang
authors: Department of Etiology and Carcinogenesis, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Kai-Tai
authors: KT
authors: Zhang
authors: State Key Laboratory of Molecular Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Ming-Rong
authors: MR
authors: Wang
authors: Department of Gynecological Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors: Ling-Ying
authors: LY
authors: Wu
keywords: Epithelial ovarian cancer; array comparative genomic hybridization; paclitaxel; platinum; recurrence