title: Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos.
abstract: The AP-1 family of transcriptional activators plays pivotal roles in regulating a wide range of biological processes from the immune response to tumorigenesis. Determining the roles of specific AP-1 dimers in cells, however, has remained challenging because common molecular biology techniques are unable to distinguish between the role of, for example, cJun/cJun homodimers versus cJun/cFos heterodimers. Here we used SELEX (systematic evolution of ligands by exponential enrichment) to identify and characterize DNA aptamers that are >100-fold more specific for binding cJun/cJun compared to cJun/cFos, setting the foundation to investigate the biological functions of different AP-1 dimer compositions.
authors: Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado, United States of America.
authors: Ryan D
authors: RD
authors: Walters
authors: Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado, United States of America.
authors: David T
authors: DT
authors: McSwiggen
authors: Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado, United States of America.
authors: James A
authors: JA
authors: Goodrich
authors: Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado, United States of America.
authors: Jennifer F
authors: JF
authors: Kugel
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001665:Binding Sites; D002460:Cell Line; D017930:Genes, Reporter; D007376:Interleukin-2 / Q000235:genetics; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D055503:Protein Multimerization; D016760:Proto-Oncogene Proteins c-fos / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D052156:SELEX Aptamer Technique; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D007376:Interleukin-2; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1