title: Stability studies of binding and functional anti-vaccine antibodies.
abstract: BACKGROUND
  As most vaccines exert their protective capacity by eliciting pathogen-specific antibodies, antibody assays assessing immunogenicity of vaccines in development should be well characterized. Part of the validation of immunogenicity assays for vaccines is the study of stability of antibodies in serum. Materials & methods: Stability of antibodies in human serum was assessed by circumsporozoite-binding IgG ELISA designed for assessing the immunogenicity of a malaria vaccine under development, adenovirus neutralization assay, designed to assess neutralizing antibodies against adenovirus and commercially available test kits for hepatitis A and B.
  
  
  RESULTS
  Stability studies indicated stability of serum-binding IgG antibodies and serum-neutralizing antibodies in: long-term storage below -65°C and -20°C; short-term storage; multiple freeze/thaw rounds; during shipment; and during heat inactivation.
  
  
  CONCLUSIONS
  RESULTS have shown the stability of both binding and functional polyclonal antibodies in human serum under stable storage and common usage circumstances.
authors: Crucell Holland BV, Archimedesweg 4-6, PO Box 2048, 2301 CA Leiden, The Netherlands.
authors: Jenny
authors: J
authors: Hendriks
authors: Coby
authors: C
authors: Stals
authors: Amanda
authors: A
authors: Versteilen
authors: Bregje
authors: B
authors: Mommaas
authors: Mariëlle
authors: M
authors: Verhoeven
authors: Felicia
authors: F
authors: Tirion
authors: Mariska Ter
authors: MT
authors: Haak
authors: Wolf
authors: W
authors: Ribbens
authors: Marije
authors: M
authors: Bosch
authors: Martijn
authors: M
authors: Trommel
authors: Stefan
authors: S
authors: Kostense
mesh_terms: D000256:Adenoviridae / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005615:Freezing; D030041:Hepatitis A virus / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D055550:Protein Stability; D015800:Protozoan Proteins / Q000276:immunology; D012044:Regression Analysis; D013696:Temperature; D013997:Time Factors; D014612:Vaccines / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D015800:Protozoan Proteins; D014612:Vaccines; C041595:circumsporozoite protein, Protozoan