title: The surface protein Lmo1941 with LysM domain influences cell wall structure and susceptibility of Listeria monocytogenes to cephalosporins.
abstract: Listeria monocytogenes is a Gram-positive bacterium causing rare but dangerous cases of disease in humans and animals. The β-lactams penicillin G and ampicillin are the antibiotics of choice in the treatment of listeriosis. Recently, lmo1941, encoding a surface protein of L. monocytogenes with unknown function, was identified as a gene transcriptionally upregulated under penicillin G pressure. In this study, the effect of lmo1941 knockout on the susceptibility of L. monocytogenes to β-lactams was examined. Deletion mutant in lmo1941 was constructed and subjected to studies, which revealed that the deletion of lmo1941 had no effect on susceptibility and tolerance to penicillin G and ampicillin but resulted, however, in increased susceptibility of L. monocytogenes to several cephalosporins. Subsequently, the potential effect of lmo1941 mutation on the cell wall of L. monocytogenes was investigated. The analysis revealed quantitative changes in the muropeptide profile of peptidoglycan and a decrease in density of the high-density zone of cell wall of the mutant strain. Both these changes were observed in cells taken from the stationary phase. These results indicate that the surface protein Lmo1941 affects peptidoglycan composition and cell wall structure of L. monocytogenes in the stationary phase of growth.
authors: Faculty of Biology, Department of Applied Microbiology, Institute of Microbiology, University of Warsaw, Warsaw, Poland.
authors: Agata
authors: A
authors: Krawczyk-Balska
authors: Dorota
authors: D
authors: Korsak
authors: Magdalena
authors: M
authors: Popowska
mesh_terms: D000667:Ampicillin / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry*; D002511:Cephalosporins / Q000494:pharmacology*; D055786:Gene Knockout Techniques; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D010400:Penicillin G / Q000494:pharmacology; D010455:Peptides / Q000032:analysis; D010457:Peptidoglycan / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002511:Cephalosporins; D008565:Membrane Proteins; D010455:Peptides; D010457:Peptidoglycan; D000667:Ampicillin; D010400:Penicillin G
keywords: peptidoglycan; susceptibility to β-lactams; tolerance to β-lactams