title: Hepatoprotective and antioxidant effect of Bauhinia hookeri extract against carbon tetrachloride-induced hepatotoxicity in mice and characterization of its bioactive compounds by HPLC-PDA-ESI-MS/MS.
abstract: The hepatoprotective and antioxidant activity of Bauhinia hookeri ethanol extract (BHE) against CCl4-induced liver injury was investigated in mice. BHE was administered (500 and 1000 mg/kg/day) along with CCl4 for 6 weeks. The hepatic marker enzymes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were determined in the serum. The antioxidant parameters: glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST), and malondialdehyde (MDA) were estimated in the liver homogenate. BHE treatment significantly inhibited the CCl4-induced increase in ALT (44 and 64%), AST (36 and 46%), ALP (28 and 42%), and MDA (39 and 51%) levels at the tested doses, respectively. Moreover, BHE treatment markedly increased the activity of antioxidant parameters GSH, GPx, GR, GST, and SOD. Histological observations confirmed the strong hepatoprotective activity. These results suggest that a dietary supplement of BHE could exert a beneficial effect against oxidative stress and various liver diseases by enhancing the antioxidant defense status, reducing lipid peroxidation, and protecting against the pathological changes of the liver. The hepatoprotective activity of BHE is mediated, at least in part, by the antioxidant effect of its constituents. The active constituents of BHE were identified by HPLC-PDA-ESI/MS/MS.
authors: Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo 11566, Egypt.
authors: Eman
authors: E
authors: Al-Sayed
authors: Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku, 20014 Turku, Finland.
authors: Olli
authors: 0000-0001-7630-9061
authors: O
authors: Martiskainen
authors: Department of Pharmacology, Theodor Bilharz Research Institute, Giza 12411, Egypt.
authors: Sayed H
authors: SH
authors: Seif el-Din
authors: Department of Pharmacology, Theodor Bilharz Research Institute, Giza 12411, Egypt.
authors: Abdel-Nasser A
authors: AN
authors: Sabra
authors: Department of Pathology, School of Medicine, Stanford University, Stanford, CA 94305, USA.
authors: Olfat A
authors: OA
authors: Hammam
authors: Department of Pharmacology, Theodor Bilharz Research Institute, Giza 12411, Egypt.
authors: Naglaa M
authors: NM
authors: El-Lakkany
authors: Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt ; Center for Emerging Infectious Diseases, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan.
authors: Mohamed M
authors: MM
authors: Abdel-Daim
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D036481:Bauhinia / Q000737:chemistry; D002251:Carbon Tetrachloride / Q000633:toxicity; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D015227:Lipid Peroxidation; D051379:Mice; D018384:Oxidative Stress; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D002251:Carbon Tetrachloride