title: Aberrant SATB1 expression is associated with Epstein-Barr virus infection, metastasis and survival in human nasopharyngeal cells and endemic nasopharyngeal carcinoma.
abstract: Special AT-rich sequence-binding protein 1 (SATB1) has been identified as a key factor in the progression of some cancers, functioning as a global genome organizer and chromatin regulator. We examined the levels of SATB1 mRNA expression in NPC cell lines 5-8F (high metastasis) and 6-10B (low metastasis) and immortalized human nasopharyngeal epithelial cells NP69-SV40T by quantitative real-time PCR. We also examined the protein expression levels of SATB1 in 72 cases of nasopharyngeal carcinoma (NPC) tissues and 30 cases of normal nasopharyngeal (NNP) tissues by immunohistochemistry, and then assessed the correlations between SATB1 expression and clinicopathological factors. The expression level of SATB1 mRNA in 5-8F was much higher than those in 6-10B and NP69-SV40T (P<0.05). The expression level of SATB1 mRNA in 6-10B was higher than in NP69-SV40T, but the difference was not statistically significant (P>0.05). The positive expression rates of SATB1 protein in NPC (38/72, 52.8%) were significantly higher than in NNP (4/30, 13.3%) (P<0.05). SATB1 protein levels in NPC were not associated with gender, age, and T stage (P>0.05), but positively correlated with the titers of EBVCA-IgA, metastasis (N and M stage), recurrence, and survival (P<0.05). Multivariate analysis showed that the overexpression of SATB1 protein is an independent prognostic factor for NPC. The expression levels of SATB1 were obviously upregulated in primary NPC tissues and human NPC cell lines. Therefore, SATB1 may be a valuable predictor in assessing the metastasis, recurrence, and prognosis of NPC.
authors: Department of Otolaryngology-Head and Neck Surgery, Zhongshan Hospital, Xiamen University Xiamen, Fujian 361004, China ; Department of Otolaryngology-Head and Neck Surgery, Union School of Clinical Medicine, Fujian Medical University Fuzhou, Fujian 350001, China.
authors: Yan-Fei
authors: YF
authors: Deng
authors: Department of Pathology, Zhongshan Hospital, Xiamen University Xiamen, Fujian 361004, China.
authors: Dong-Ni
authors: DN
authors: Zhou
authors: Department of Otolaryngology-Head and Neck Surgery, Union School of Clinical Medicine, Fujian Medical University Fuzhou, Fujian 350001, China.
authors: Zhi-Yong
authors: ZY
authors: Pan
authors: Department of Pathology, Zhongshan Hospital, Xiamen University Xiamen, Fujian 361004, China.
authors: Ping
authors: P
authors: Yin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001706:Biopsy; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000556:secondary / Q000821:virology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D018572:Disease-Free Survival; D019353:Endemic Diseases; D020031:Epstein-Barr Virus Infections / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000821:virology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D015999:Multivariate Analysis; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000821:virology; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D036961:Matrix Attachment Region Binding Proteins; D012333:RNA, Messenger; C076189:SATB1 protein, human
keywords: Nasopharyngeal carcinoma; SATB1; immunohistochemistry; prognosis; quantitative real-time PCR