title: Two-step phase-shifting fluorescence incoherent holographic microscopy.
abstract: Fluorescence holographic microscope (FINCHSCOPE) is a motionless fluorescence holographic imaging technique based on Fresnel incoherent correlation holography (FINCH) that shows promise in reconstructing three-dimensional fluorescence images of biological specimens with three holograms. We report a developing two-step phase-shifting method that reduces the required number of holograms from three to two. Using this method, we resolved microscopic fluorescent beads that were three-dimensionally distributed at different depths with two interferograms captured by a CCD camera. The method enables the FINCHSCOPE to work in conjunction with the frame-straddling technique and significantly enhance imaging speed.
authors: Clemson University, Department of Bioengineering and COMSET, Clemson, South Carolina 29634.
authors: Wan
authors: W
authors: Qin
authors: Clemson University, Department of Bioengineering and COMSET, Clemson, South Carolina 29634.
authors: Xiaoqi
authors: X
authors: Yang
authors: Shenzhen University, Institute of Optoelectronics, Shenzhen, Guangdong 518060, China.
authors: Yingying
authors: Y
authors: Li
authors: Shenzhen University, Institute of Optoelectronics, Shenzhen, Guangdong 518060, China.
authors: Xiang
authors: X
authors: Peng
authors: Clemson University, Department of Bioengineering and COMSET, Clemson, South Carolina 29634.
authors: Hai
authors: H
authors: Yao
authors: Tianjin University, State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin 300072, China.
authors: Xinghua
authors: X
authors: Qu
authors: Clemson University, Department of Bioengineering and COMSET, Clemson, South Carolina 29634.
authors: Bruce Z
authors: BZ
authors: Gao
mesh_terms: D005583:Fourier Analysis; D006696:Holography / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007368:Interferometry / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D012815:Signal Processing, Computer-Assisted
chemical_list: D011108:Polymers