title: Hydrogen peroxide production and mitochondrial dysfunction contribute to the fusaric acid-induced programmed cell death in tobacco cells.
abstract: Fusaric acid (FA), a non-specific toxin produced mainly by Fusarium spp., can cause programmed cell death (PCD) in tobacco suspension cells. The mechanism underlying the FA-induced PCD was not well understood. In this study, we analyzed the roles of hydrogen peroxide (H2O2) and mitochondrial function in the FA-induced PCD. Tobacco suspension cells were treated with 100 μM FA and then analyzed for H2O2 accumulation and mitochondrial functions. Here we demonstrate that cells undergoing FA-induced PCD exhibited H2O2 production, lipid peroxidation, and a decrease of the catalase and ascorbate peroxidase activities. Pre-treatment of tobacco suspension cells with antioxidant ascorbic acid and NADPH oxidase inhibitor diphenyl iodonium significantly reduced the rate of FA-induced cell death as well as the caspase-3-like protease activity. Moreover, FA treatment of tobacco cells decreased the mitochondrial membrane potential and ATP content. Oligomycin and cyclosporine A, inhibitors of the mitochondrial ATP synthase and the mitochondrial permeability transition pore, respectively, could also reduce the rate of FA-induced cell death significantly. Taken together, the results presented in this paper demonstrate that H2O2 accumulation and mitochondrial dysfunction are the crucial events during the FA-induced PCD in tobacco suspension cells.
authors: Department of Plant Pathology, Shandong Agricultural University, Taian 271018, Shandong Province, China.
authors: Jiao
authors: J
authors: Jiao
authors: Department of Plant Pathology, Shandong Agricultural University, Taian 271018, Shandong Province, China.
authors: Ling
authors: L
authors: Sun
authors: Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui Province, China.
authors: Benguo
authors: B
authors: Zhou
authors: Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui Province, China.
authors: Zhengliang
authors: Z
authors: Gao
authors: Department of Plant Pathology, Shandong Agricultural University, Taian 271018, Shandong Province, China.
authors: Yu
authors: Y
authors: Hao
authors: Department of Plant Pathology, Shandong Agricultural University, Taian 271018, Shandong Province, China.
authors: Xiaoping
authors: X
authors: Zhu
authors: Department of Plant Pathology, Shandong Agricultural University, Taian 271018, Shandong Province, China. Electronic address: liangyc@sdau.edu.cn.
authors: Yuancun
authors: Y
authors: Liang
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology; D005669:Fusaric Acid / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D009840:Oligomycins / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D009840:Oligomycins; D010940:Plant Proteins; D016572:Cyclosporine; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D002374:Catalase; D053148:Caspase 3; D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases; D005669:Fusaric Acid
keywords: Fusaric acid; Hydrogen peroxide; Mitochondrion; Programmed cell death; Tobacco suspension cells