title: Maternally acquired genotoxic Escherichia coli alters offspring's intestinal homeostasis.
abstract: The neonatal gut is rapidly colonized by a newly dominant group of commensal Escherichia coli strains among which a large proportion produces a genotoxin called colibactin. In order to analyze the short- and long-term effects resulting from such evolution, we developed a rat model mimicking the natural transmission of E. coli from mothers to neonates. Genotoxic and non-genotoxic E. coli strains were equally transmitted to the offspring and stably colonized the gut across generations. DNA damage was only detected in neonates colonized with genotoxic E. coli strains. Signs of genotoxic stress such as anaphase bridges, higher occurrence of crypt fission and accelerated renewal of the mature epithelium were detected at adulthood. In addition, we observed alterations of secretory cell populations and gut epithelial barrier. Our findings illustrate how critical is the genotype of E. coli strains acquired at birth for gut homeostasis at adulthood.
authors: INRA; USC 1360; Toulouse, France; Inserm; UMR1043; Toulouse, France; CNRS; UMR5282; Toulouse, France; Université de Toulouse; UPS; Toulouse, France; Neuro-gastroenterologie & Nutrition; UMR Toxalim INRA/ENVT 1331; Toulouse, France.
authors: Delphine
authors: D
authors: Payros
authors: INRA; USC 1360; Toulouse, France; Inserm; UMR1043; Toulouse, France; CNRS; UMR5282; Toulouse, France; Université de Toulouse; UPS; Toulouse, France.
authors: Thomas
authors: T
authors: Secher
authors: INRA; USC 1360; Toulouse, France; Inserm; UMR1043; Toulouse, France; CNRS; UMR5282; Toulouse, France; Université de Toulouse; UPS; Toulouse, France.
authors: Michèle
authors: M
authors: Boury
authors: INRA; USC 1360; Toulouse, France; Inserm; UMR1043; Toulouse, France; CNRS; UMR5282; Toulouse, France; Université de Toulouse; UPS; Toulouse, France; CHU Toulouse; Hôpital Purpan; Service de bactériologie-Hygiène; Toulouse, France.
authors: Camille
authors: C
authors: Brehin
authors: Neuro-gastroenterologie & Nutrition; UMR Toxalim INRA/ENVT 1331; Toulouse, France.
authors: Sandrine
authors: S
authors: Ménard
authors: Neuro-gastroenterologie & Nutrition; UMR Toxalim INRA/ENVT 1331; Toulouse, France.
authors: Christel
authors: C
authors: Salvador-Cartier
authors: INRA; USC 1360; Toulouse, France; Inserm; UMR1043; Toulouse, France; CNRS; UMR5282; Toulouse, France; Université de Toulouse; UPS; Toulouse, France.
authors: Gabriel
authors: G
authors: Cuevas-Ramos
authors: INRA; USC 1360; Toulouse, France; Inserm; UMR1043; Toulouse, France; CNRS; UMR5282; Toulouse, France; Université de Toulouse; UPS; Toulouse, France.
authors: Claude
authors: C
authors: Watrin
authors: Université de Picardie Jules Verne; EA 4666 UFR de médecine d'Amiens, France.
authors: Ingrid
authors: I
authors: Marcq
authors: INRA; USC 1360; Toulouse, France; Inserm; UMR1043; Toulouse, France; CNRS; UMR5282; Toulouse, France; Université de Toulouse; UPS; Toulouse, France.
authors: Jean-Philippe
authors: JP
authors: Nougayrède
authors: INRA; USC 1360; Toulouse, France; Inserm; UMR1043; Toulouse, France; CNRS; UMR5282; Toulouse, France; Université de Toulouse; UPS; Toulouse, France; CHU Toulouse; Hôpital Purpan; Service de bactériologie-Hygiène; Toulouse, France.
authors: Damien
authors: D
authors: Dubois
authors: CHU Limoge; Service de Pédiatrie; Limoges, France.
authors: Antoine
authors: A
authors: Bedu
authors: Université de Limoges UMR-S1092; Limoges, France; Inserm U1092; Limoges, France.
authors: Fabien
authors: F
authors: Garnier
authors: Inserm UMR-S 722; Univ Paris Diderot; PRES Sorbonne Cité; Paris, France.
authors: Olivier
authors: O
authors: Clermont
authors: Inserm UMR-S 722; Univ Paris Diderot; PRES Sorbonne Cité; Paris, France.
authors: Erick
authors: E
authors: Denamur
authors: INRA USC 1235; Inserm U1060; CarMeN laboratory; Villeurbanne, France.
authors: Pascale
authors: P
authors: Plaisancié
authors: Neuro-gastroenterologie & Nutrition; UMR Toxalim INRA/ENVT 1331; Toulouse, France.
authors: Vassilia
authors: V
authors: Theodorou
authors: Neuro-gastroenterologie & Nutrition; UMR Toxalim INRA/ENVT 1331; Toulouse, France.
authors: Jean
authors: J
authors: Fioramonti
authors: INRA; USC 1360; Toulouse, France; Inserm; UMR1043; Toulouse, France; CNRS; UMR5282; Toulouse, France; Université de Toulouse; UPS; Toulouse, France; Neuro-gastroenterologie & Nutrition; UMR Toxalim INRA/ENVT 1331; Toulouse, France.
authors: Maïwenn
authors: M
authors: Olier
authors: INRA; USC 1360; Toulouse, France; Inserm; UMR1043; Toulouse, France; CNRS; UMR5282; Toulouse, France; Université de Toulouse; UPS; Toulouse, France; CHU Toulouse; Hôpital Purpan; Service de bactériologie-Hygiène; Toulouse, France.
authors: Eric
authors: E
authors: Oswald
mesh_terms: D000818:Animals; D004249:DNA Damage / Q000187:drug effects*; D004926:Escherichia coli / Q000378:metabolism*; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D023421:Models, Animal; D010455:Peptides / Q000378:metabolism*; D061065:Polyketides / Q000378:metabolism*; D011247:Pregnancy; D017208:Rats, Wistar
chemical_list: D010455:Peptides; D061065:Polyketides; C569566:colibactin
keywords: Escherichia coli; colibactin; epithelial differentiation; epithelial proliferation; genotoxicity; gut; intestinal barrier; neonate