title: Effect of dietary supplementation with flaxseed oil or vitamin E on sheep experimentally infected with Fasciola hepatica.
abstract: The effects of two diets supplemented with flaxseed oil (FO) or vitamin E (VE) were studied in sheep infected with Fasciola hepatica. The control group (CO), not supplemented, had higher weight gain and faecal egg count (P < 0.05) at the end of the study. At necropsy, the number (71.2 ± 26.5) and size of flukes were lower in the FO group (P < 0.01), probably due to the higher levels of white blood cells and lymphocytes (P < 0.01). This group also had higher red blood cell and haematocrit values (P < 0.01). The CO group had more severe hypoproteinaemia and hypoalbuminaemia, due to having the highest fluke burden (123.0 ± 35.2), and also the highest IgG1 titre (P < 0.01). The diet did not affect production or gene expression levels of IFN-γ and IL-4. On the other hand, the supplementation with VE led to a reduction in adult fluke burden (97.7 ± 39.9) and lower lipid oxidation in the liver (P < 0.001).
authors: Departamento de Sanidad Animal, Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de León, 24071 León, Spain.
authors: J M
authors: JM
authors: Martínez-Pérez
authors: Departamento de Sanidad Animal, Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de León, 24071 León, Spain.
authors: D
authors: D
authors: Robles-Pérez
authors: Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, 24346 Grulleros (León), Spain.
authors: J
authors: J
authors: Benavides
authors: Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, 24346 Grulleros (León), Spain.
authors: L
authors: L
authors: Morán
authors: Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, 24346 Grulleros (León), Spain.
authors: S
authors: S
authors: Andrés
authors: Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, 24346 Grulleros (León), Spain.
authors: F J
authors: FJ
authors: Giráldez
authors: Departamento de Sanidad Animal, Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de León, 24071 León, Spain; Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, 24346 Grulleros (León), Spain.
authors: F A
authors: FA
authors: Rojo-Vázquez
authors: Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, 24346 Grulleros (León), Spain. Electronic address: mmarva@unileon.es.
authors: M
authors: M
authors: Martínez-Valladares
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001824:Body Constitution / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D017931:DNA Primers / Q000235:genetics; D019587:Dietary Supplements; D005210:Fasciola hepatica / Q000187:drug effects*; D005211:Fascioliasis / Q000097:blood / Q000178:diet therapy / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008043:Linseed Oil / Q000008:administration & dosage*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010270:Parasite Egg Count / Q000662:veterinary; D060888:Real-Time Polymerase Chain Reaction; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000178:diet therapy* / Q000469:parasitology*; D034561:Sheep, Domestic; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E / Q000008:administration & dosage*
chemical_list: D017931:DNA Primers; D007074:Immunoglobulin G; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D015847:Interleukin-4; D008043:Linseed Oil; D007371:Interferon-gamma
keywords: Fasciola hepatica; Flaxseed oil; Immune system; Sheep; Vitamin E