title: Optimization of fed-batch enzymatic hydrolysis from alkali-pretreated sugarcane bagasse for high-concentration sugar production.
abstract: Fed-batch enzymatic hydrolysis process from alkali-pretreated sugarcane bagasse was investigated to increase solids loading, produce high-concentration fermentable sugar and finally to reduce the cost of the production process. The optimal initial solids loading, feeding time and quantities were examined. The hydrolysis system was initiated with 12% (w/v) solids loading in flasks, where 7% fresh solids were fed consecutively at 6h, 12h, 24h to get a final solids loading of 33%. All the requested cellulase loading (10 FPU/g substrate) was added completely at the beginning of hydrolysis reaction. After 120 h of hydrolysis, the maximal concentrations of cellobiose, glucose and xylose obtained were 9.376 g/L, 129.50 g/L, 56.03 g/L, respectively. The final total glucan conversion rate attained to 60% from this fed-batch process.
authors: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Yueshu
authors: Y
authors: Gao
authors: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors: Jingliang
authors: J
authors: Xu
authors: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China. Electronic address: yuanzh@ms.giec.ac.cn.
authors: Zhenhong
authors: Z
authors: Yuan
authors: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors: Yu
authors: Y
authors: Zhang
authors: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors: Yunyun
authors: Y
authors: Liu
authors: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors: Cuiyi
authors: C
authors: Liang
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D002241:Carbohydrates / Q000096:biosynthesis*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000494:pharmacology*; D005936:Glucans / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis / Q000187:drug effects; D013997:Time Factors; D014990:Xylans / Q000032:analysis; D014994:Xylose / Q000032:analysis
chemical_list: D000468:Alkalies; D002241:Carbohydrates; D005936:Glucans; D014990:Xylans; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D002480:Cellulase; D005947:Glucose
keywords: Enzymatic hydrolysis; Fed-batch process; High-concentration sugars; Sugarcane bagasse