title: Ectopic over-expression of peroxisomal ascorbate peroxidase (SbpAPX) gene confers salt stress tolerance in transgenic peanut (Arachis hypogaea).
abstract: Peroxisomal ascorbate peroxidase gene (SbpAPX) of an extreme halophyte Salicornia brachiata imparts abiotic stress endurance and plays a key role in the protection against oxidative stress. The cloned SbpAPX gene was transformed to local variety of peanut and about 100 transgenic plants were developed using optimized in vitro regeneration and Agrobacterium mediated genetic transformation method. The T0 transgenic plants were confirmed for the gene integration; grown under controlled condition in containment green house facility; seeds were harvested and T1 plants were raised. Transgenic plants (T1) were further confirmed by PCR using gene specific primers and histochemical GUS assay. About 40 transgenic plants (T1) were selected randomly and subjected for salt stress tolerance study. Transgenic plants remained green however non-transgenic plants showed bleaching and yellowish leaves under salt stress conditions. Under stress condition, transgenic plants continued normal growth and completed their life cycle. Transgenic peanut plants exhibited adequate tolerance under salt stress condition and thus could be explored for the cultivation in salt affected areas for the sustainable agriculture.
authors: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar, 364 002 Gujarat, India. Electronic address: nsnahar@gmail.com.
authors: Natwar
authors: N
authors: Singh
authors: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar, 364 002 Gujarat, India. Electronic address: avinash@csmcri.org.
authors: Avinash
authors: A
authors: Mishra
authors: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar, 364 002 Gujarat, India. Electronic address: bjha@csmcri.org.
authors: Bhavanath
authors: B
authors: Jha
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D060054:Agrobacterium / Q000235:genetics; D010367:Arachis / Q000235:genetics / Q000502:physiology*; D060387:Ascorbate Peroxidases / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D020675:Peroxisomes / Q000201:enzymology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology; D016133:Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology*; D014170:Transformation, Genetic
chemical_list: D017931:DNA Primers; D012965:Sodium Chloride; D060387:Ascorbate Peroxidases
keywords: Groundnut; Halophyte; Salinity; Transformation; Transgenic plants