title: Implantation of inferior vena cava interposition graft in mouse model.
abstract: Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. The long-term clinical results showed excellent patency rates, however, with significant incidence of stenosis. To investigate the cellular and molecular mechanisms of vascular neotissue formation and prevent stenosis development in tissue engineered vascular grafts (TEVGs), we developed a mouse model of the graft with approximately 1 mm internal diameter. First, the TEVGs were assembled from biodegradable tubular scaffolds fabricated from a polyglycolic acid nonwoven felt mesh coated with ε-caprolactone and L-lactide copolymer. The scaffolds were then placed in a lyophilizer, vacuumed for 24 hr, and stored in a desiccator until cell seeding. Second, bone marrow was collected from donor mice and mononuclear cells were isolated by density gradient centrifugation. Third, approximately one million cells were seeded on a scaffold and incubated O/N. Finally, the seeded scaffolds were then implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. The implanted grafts demonstrated excellent patency (>90%) without evidence of thromboembolic complications or aneurysmal formation. This murine model will aid us in understanding and quantifying the cellular and molecular mechanisms of neotissue formation in the TEVG.
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital; yongung.lee@nationwidechildrens.org.
authors: Yong-Ung
authors: YU
authors: Lee
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Tai
authors: T
authors: Yi
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Shuhei
authors: S
authors: Tara
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Avione Y
authors: AY
authors: Lee
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Narutoshi
authors: N
authors: Hibino
authors: Department of Cardiothoracic Surgery, Nationwide Children's Hospital.
authors: Toshiharu
authors: T
authors: Shinoka
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital; Pediatric Surgery, Nationwide Children's Hospital.
authors: Christopher K
authors: CK
authors: Breuer
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D001854:Bone Marrow Cells / Q000166:cytology; D005260:Female; D006085:Graft Survival / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D011091:Polyesters; D011100:Polyglycolic Acid; D054457:Tissue Scaffolds; D014682:Vena Cava, Inferior / Q000166:cytology / Q000601:surgery*
chemical_list: D011091:Polyesters; D011100:Polyglycolic Acid; C062968:lactide-caprolactone copolymer