title: Polymeric vector-mediated gene transfection of MSCs for dual bioluminescent and MRI tracking in vivo.
abstract: MSC's transplantation is a promising cell-based therapy for injuries in regenerative medicine, and in vivo visualization of transplanted MSCs with noninvasive technique is essential for the tracking of cell infusion and homing. A new cationic polymer, poly(ethylene glycol)-block-poly(l-aspartic acid)-grafted polyethylenimine functionalized with superparamagnetic iron oxide nanoparticles (PAI/SPION), was constructed as a magnetic resonance imaging (MRI)-visible non-viral vector for the delivery of plasmids DNA (pDNA) encoding for luciferase and red fluorescence protein (RFP) as reporter genes into MSCs. As a result, the MSCs were labeled with SPION and reporter genes. The PAI/SPION complexes exhibited high transfection efficiency in transferring pDNA into MSCs, which resulted in efficient luciferase and RFP co-expression. Furthermore, the complexes did not significantly affect the viability and multilineage differentiation capacity of MSCs. After the labeled MSCs were transplanted into the rats with acute liver injury via the superior mesenteric vein (SMV) injection, the migration behavior and organ-specific accumulation of the cells could be effectively monitored using the in vivo imaging system (IVIS) and MRI, respectively. The immunohistochemical analysis further confirmed that the transplanted MSCs were predominantly distributed in the liver parenchyma. Our results indicate that the PAI/SPION is a MRI-visible gene delivery agent which can effectively label MSCs to provide the basis for bimodal bioluminescence and MRI tracking in vivo.
authors: Molecular Imaging Lab, Department of Radiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Interventional Radiology Institute, Sun Yat-sen University, Guangzhou 510630, China.
authors: Chun
authors: C
authors: Wu
authors: PCFM Lab of Ministry of Education, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors: Jingguo
authors: J
authors: Li
authors: Molecular Imaging Lab, Department of Radiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Interventional Radiology Institute, Sun Yat-sen University, Guangzhou 510630, China.
authors: Pengfei
authors: P
authors: Pang
authors: Molecular Imaging Lab, Department of Radiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors: Jingjing
authors: J
authors: Liu
authors: Molecular Imaging Lab, Department of Radiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Interventional Radiology Institute, Sun Yat-sen University, Guangzhou 510630, China.
authors: Kangshun
authors: K
authors: Zhu
authors: Molecular Imaging Lab, Department of Radiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors: Dan
authors: D
authors: Li
authors: PCFM Lab of Ministry of Education, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors: Du
authors: D
authors: Cheng
authors: Molecular Imaging Lab, Department of Radiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Interventional Radiology Institute, Sun Yat-sen University, Guangzhou 510630, China.
authors: Junwei
authors: J
authors: Chen
authors: PCFM Lab of Ministry of Education, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: shuaixt@mail.sysu.edu.cn.
authors: Xintao
authors: X
authors: Shuai
authors: Molecular Imaging Lab, Department of Radiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Interventional Radiology Institute, Sun Yat-sen University, Guangzhou 510630, China. Electronic address: shanhong@mail.sysu.edu.cn.
authors: Hong
authors: H
authors: Shan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002412:Cations; D002454:Cell Differentiation; D002465:Cell Movement; D003911:Dextrans / Q000737:chemistry; D005260:Female; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D007150:Immunohistochemistry; D008099:Liver / Q000293:injuries; D049449:Luminescence; D008164:Luminescent Proteins / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053768:Metal Nanoparticles; D010957:Plasmids / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection / Q000379:methods*; D000097573:Red Fluorescent Protein
chemical_list: D001672:Biocompatible Materials; D002412:Cations; D003911:Dextrans; D008164:Luminescent Proteins; D058185:Magnetite Nanoparticles; D000097573:Red Fluorescent Protein; C065507:ferumoxides
keywords: Acute liver injury; Bioluminescence; Magnetic resonance imaging; Mesenchymal stem cells; Reporter gene