title: Development of loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of ostrich meat.
abstract: Animal species identification is one of the primary duties of official food control. Since ostrich meat is difficult to be differentiated macroscopically from beef, therefore new analytical methods are needed. To enforce labeling regulations for the authentication of ostrich meat, it might be of importance to develop and evaluate a rapid and reliable assay. In the present study, a loop-mediated isothermal amplification (LAMP) assay based on the cytochrome b gene of the mitochondrial DNA of the species Struthio camelus was developed. The LAMP assay was used in combination with a real-time fluorometer. The developed system allowed the detection of 0.01% ostrich meat products. In parallel, a direct swab method without nucleic acid extraction using the HYPLEX LPTV buffer was also evaluated. This rapid processing method allowed detection of ostrich meat without major incubation steps. In summary, the LAMP assay had excellent sensitivity and specificity for detecting ostrich meat and could provide a sampling-to-result identification-time of 15 to 20 minutes.
authors: Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Hannover, Germany.
authors: Amir
authors: A
authors: Abdulmawjood
authors: Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Hannover, Germany.
authors: Nils
authors: N
authors: Grabowski
authors: Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Hannover, Germany.
authors: Svenja
authors: S
authors: Fohler
authors: Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Hannover, Germany.
authors: Sophie
authors: S
authors: Kittler
authors: Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Hannover, Germany.
authors: Helga
authors: H
authors: Nagengast
authors: Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Hannover, Germany.
authors: Guenter
authors: G
authors: Klein
mesh_terms: D000818:Animals; D045303:Cytochromes b / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D063427:Food Quality; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010802:Phylogeny; D020310:Struthioniformes / Q000145:classification / Q000235:genetics*; D013997:Time Factors
chemical_list: D004272:DNA, Mitochondrial; D045303:Cytochromes b