title: [Oleanolic acid synergizes with cyclosporine A to prolong renal allograft survival in rats].
abstract: OBJECTIVE
  To investigate the synergistic effect of oleanolic acid (OA) and cyclosporine A (CsA) on the survival of renal allografts in rats.
  
  
  METHODS
  Renal allograft transplantation was performed using BN rats as donors and LEW rats as recipients. Forty male LEW rats were randomized into 4 equal groups for interventions with DMSO-PBS (control), OA, CsA, or CsA+OA, starting from 1 day before transplantation. Serum creatinine levels were regularly examined, and the survival of rats were recorded. On day 5 after transplantation, CD4(+) and CD8(+) T-cell infiltration in the renal grafts was analyzed by immunohistochemistry; the concentrations of the proinflammatory cytokines (IL-1β, IFN-γ, IL-2, IL-4, and IL-17), anti-inflammatory cytokine IL-10 and chemokines (IP-10, MCP-1, MIP, and Mig) were analyzed with Luminex; the T-cell phenotypes (IFN-γ, IL-10, IL-4, and IL-17) were analyzed using ELISpot.
  
  
  RESULTS
  In OA+CsA group, renal allograft survival was markedly prolonged and CD4(+) and CD8(+) T cell infiltration in the graft significantly decreased as compared to other groups. A significant decrease in IL-2 was observed in OA group and OA+CsA group, especially the latter. Compared with the control group, all the 3 treated groups showed significantly decreased IL-1β, IP-10 and MCP-1, increased IL-10 levels, decreased percentages of T cells secreting IFN-γ, IL-4 and IL-17, and increased percentage of T cells secreting IL-10. The increments of serum IL-10 level and T cell percentage were more prominent in OA+CsA group than in the other two intervention groups.
  
  
  CONCLUSIONS
  OA and CsA synergistically ameliorate renal graft rejection and inflammation and promote allograft survival and function in rats.
authors: Center of Kidney Transplantation, Second Affiliated Hospital of University of South China, Hengyang 421001, China.E-mail: qianyihaojn@sina.com.
authors: Kun
authors: K
authors: Qian
authors: Wenting
authors: W
authors: Liao
authors: Jianjun
authors: J
authors: Li
authors: Hongtao
authors: H
authors: Jiang
authors: Hao
authors: H
authors: Zhou
authors: Jianhua
authors: J
authors: Long
authors: Guoqing
authors: G
authors: Qin
authors: Yi
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004357:Drug Synergism; D006085:Graft Survival; D007668:Kidney / Q000187:drug effects; D016030:Kidney Transplantation; D008297:Male; D009828:Oleanolic Acid / Q000494:pharmacology*; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000166:cytology; D014184:Transplantation, Homologous
chemical_list: D016207:Cytokines; D009828:Oleanolic Acid; D016572:Cyclosporine