title: Spectroscopic study on the interaction between naphthalimide-polyamine conjugates and DNA.
abstract: The interaction of naphthalimide-polyamine conjugates with herring sperm DNA was studied by UV/vis absorption and fluorescent spectra under physiological conditions (pH=7.4). The observed spectral quenching of compounds by DNA and the displacement of EB from DNA-EB complex by compounds indicated that these naphthalimide-polyamine conjugates could intercalate into the DNA base pairs. The UV test also showed that these compounds caused the conformational alteration of DNA. Further caloric fluorescent tests revealed that the quenching mechanism was a static type, which Ksv of 1-DNA, 2-DNA and 1-DNA-EB, 2-DNA-EB 3-DNA-EB was 1.208×10(4), 7.792×10(3) and 1.712×10(4), 1.287×10(4), 2.874×10(4), respectively, at room temperature. The obtained quenching constant, binding constant and thermodynamic parameters suggested that binding strength was associated with substituted groups on naphthalene backbone, and the type of interaction force included mainly hydrogen bonding and weak van der Waals. The binding process was mainly driven by hydrogen bond and van der Waals. Additionally, the effect of NaCl on compounds-DNA interaction provided further evidence that their interaction modes were dependent on substituted groups.
authors: Institute of Chemical Biology, Henan University, Kaifeng 475004, China. Electronic address: tzynew@sohu.com.
authors: Zhiyong
authors: Z
authors: Tian
authors: Institute of Chemical Biology, Henan University, Kaifeng 475004, China.
authors: Zhonghua
authors: Z
authors: Zhao
authors: Institute of Chemical Biology, Henan University, Kaifeng 475004, China.
authors: Fenglei
authors: F
authors: Zang
authors: Institute of Chemical Biology, Henan University, Kaifeng 475004, China.
authors: Yueqiao
authors: Y
authors: Wang
authors: The Key Laboratory of Natural Medicine and Immuno-Engineering, Henan University, Kaifeng 475004, China. Electronic address: wcjsxq@henu.edu.cn.
authors: Chaojie
authors: C
authors: Wang
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry*; D005399:Fishes; D006860:Hydrogen Bonding; D008297:Male; D053644:Naphthalimides / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013094:Spermatozoa / Q000378:metabolism; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: D053644:Naphthalimides; D011073:Polyamines; D004247:DNA
keywords: DNA; Naphthalimides; Polyamine conjugates; Spectroscopic methods