title: Chronic lymphocytic leukaemia cells become both activated and immunosuppressive following interaction with CD3 and CD28 stimulated PBMC.
abstract: Chronic lymphocytic leukaemia (CLL) is associated with immunosuppression. The activation of CLL cells induced by interaction with other cell types, particularly activated T-cells, within the tumour micro-environment is thought to be important for CLL progression. However it is unclear whether activated CLL cells (CLL(Act)) have immunosuppressive capacity. We report that co-culture of CLL cells with normal PBMC in the context of CD3/CD28 T-cell activation generates CLL(Act) with increased CD38 expression that are capable of suppressing the proliferative responses of both CD4+ and CD8+ T-cells. The suppression required cell contact but did not involve induction of T-cell apoptosis.
authors: Haematology Research Group, Christchurch Hospital, Christchurch, New Zealand; Pathology Department, University of Otago, Christchurch, New Zealand. Electronic address: barry.hock@otago.ac.nz.
authors: B D
authors: BD
authors: Hock
authors: Haematology Department, Christchurch Hospital, Christchurch, New Zealand; Pathology Department, University of Otago, Christchurch, New Zealand.
authors: S A
authors: SA
authors: Macpherson
authors: Haematology Department, Christchurch Hospital, Christchurch, New Zealand; Pathology Department, University of Otago, Christchurch, New Zealand.
authors: L J
authors: LJ
authors: Fernyhough
authors: Haematology Research Group, Christchurch Hospital, Christchurch, New Zealand; Pathology Department, University of Otago, Christchurch, New Zealand.
authors: J L
authors: JL
authors: McKenzie
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D049109:Cell Proliferation; D018920:Coculture Techniques; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged
chemical_list: D018106:CD28 Antigens; D017252:CD3 Complex
keywords: Activation; Chronic lymphocytic leukaemia; Immunosuppression; Micro-environment; T-cell