title: Spatio-temporal mapping of variation potentials in leaves of Helianthus annuus L. seedlings in situ using multi-electrode array.
abstract: Damaging thermal stimuli trigger long-lasting variation potentials (VPs) in higher plants. Owing to limitations in conventional plant electrophysiological recording techniques, recorded signals are composed of signals originating from all of the cells that are connected to an electrode. This limitation does not enable detailed spatio-temporal distributions of transmission and electrical activities in plants to be visualised. Multi-electrode array (MEA) enables the recording and imaging of dynamic spatio-temporal electrical activities in higher plants. Here, we used an 8 × 8 MEA with a polar distance of 450 μm to measure electrical activities from numerous cells simultaneously. The mapping of the data that were recorded from the MEA revealed the transfer mode of the thermally induced VPs in the leaves of Helianthus annuus L. seedlings in situ. These results suggest that MEA can enable recordings with high spatio-temporal resolution that facilitate the determination of the bioelectrical response mode of higher plants under stress.
authors: College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.
authors: Dong-Jie
authors: DJ
authors: Zhao
authors: College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.
authors: Zhong-Yi
authors: ZY
authors: Wang
authors: College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.
authors: Lan
authors: L
authors: Huang
authors: Bio-gene Technology Ltd., Beijing 100124, China.
authors: Yong-Peng
authors: YP
authors: Jia
authors: Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA.
authors: John Q
authors: JQ
authors: Leng
mesh_terms: D002121:Calcium Channel Blockers / Q000494:pharmacology; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006368:Helianthus / Q000166:cytology / Q000502:physiology*; D007811:Lanthanum / Q000494:pharmacology; D059828:Plant Cells / Q000187:drug effects / Q000502:physiology*; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D026902:Potassium Channel Blockers / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism; D015203:Reproducibility of Results; D036226:Seedlings / Q000166:cytology / Q000502:physiology*; D013696:Temperature; D019789:Tetraethylammonium / Q000494:pharmacology; D013997:Time Factors; D014638:Vanadates / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D026902:Potassium Channel Blockers; C028521:lanthanum chloride; D014638:Vanadates; D019789:Tetraethylammonium; D007811:Lanthanum; D006180:Proton-Translocating ATPases