title: Dehydroabietic acid isolated from Commiphora opobalsamum causes endothelium-dependent relaxation of pulmonary artery via PI3K/Akt-eNOS signaling pathway.
abstract: Commiphora opobalsamum is a Traditional Chinese Medicine used to treat traumatic injury, mainly by relaxing blood vessels. In this study, two diterpenes, dehydroabietic acid (DA) and sandaracopimaric acid (SA) were obtained from it by a bioassay-guided approach using isolated rat pulmonary artery rings. The structures of the two compounds were elucidated by spectroscopic methods (IR, 1H- and 13C-NMR, HR-ESI-MS). Both DA and SA reduced the contraction of phenylephrine-induced pulmonary arteries in a concentration-dependent manner, and endothelium contributed greatly to the vasodilatory effect of DA. This effect of DA was attenuated by NG-Nitro-L-arginine methyl ester (L-NAME, an eNOS inhibitor). Meanwhile, DA increased nitric oxide (NO) production, along with the increase of phosphorylation level of eNOS and Akt in endothelial cells. LY294002 (a PI3K inhibitor) could reverse this effect, which suggested the endothelial PI3K/Akt pathway involved in the mechanism underlying DA-induced relaxation of pulmonary artery. This work provided evidence of vasorelaxant substances in Commiphora opobalsamum and validated that PI3K/Akt-eNOS pathway was associated with DA-induced pulmonary artery vasodilation.
authors: College of Pharmacy, Harbin Medical University-Daqing, Daqing 163319, Heilongjiang, China. gaowenyan1987@126.com.
authors: Wenyan
authors: W
authors: Gao
authors: College of Pharmacy, Harbin Medical University-Daqing, Daqing 163319, Heilongjiang, China. remembertry@163.com.
authors: Xiaoyan
authors: X
authors: Dong
authors: College of Pharmacy, Harbin University of Commerce, Harbin 150028, Heilongjiang, China. xienan2002@126.com.
authors: Nan
authors: N
authors: Xie
authors: College of Pharmacy, Harbin Medical University-Daqing, Daqing 163319, Heilongjiang, China. zhouchunlan490326@163.com.
authors: Chunlan
authors: C
authors: Zhou
authors: College of Pharmacy, Harbin Medical University-Daqing, Daqing 163319, Heilongjiang, China. fyh198306@126.com.
authors: Yuhua
authors: Y
authors: Fan
authors: College of Pharmacy, Harbin Medical University-Daqing, Daqing 163319, Heilongjiang, China. cgy20050101@126.com.
authors: Guoyou
authors: G
authors: Chen
authors: College of Pharmacy, Harbin Medical University-Daqing, Daqing 163319, Heilongjiang, China. wymingming@163.com.
authors: Yanming
authors: Y
authors: Wang
authors: College of Pharmacy, Harbin Medical University-Daqing, Daqing 163319, Heilongjiang, China. genwy12345@126.com.
authors: Taiming
authors: T
authors: Wei
authors: College of Pharmacy, Harbin Medical University-Daqing, Daqing 163319, Heilongjiang, China. dalingzhu2000@163.com.
authors: Daling
authors: D
authors: Zhu
mesh_terms: D045784:Abietanes / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002867:Chromones / Q000494:pharmacology; D029744:Commiphora / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D042783:Endothelial Cells / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D008516:Medicine, Chinese Traditional; D009025:Morpholines / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011651:Pulmonary Artery / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D045784:Abietanes; D002867:Chromones; D004224:Diterpenes; D004791:Enzyme Inhibitors; D009025:Morpholines; D000081082:Phosphoinositide-3 Kinase Inhibitors; C013913:dehydroabietic acid; D009569:Nitric Oxide; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D058539:Phosphatidylinositol 3-Kinase; D051057:Proto-Oncogene Proteins c-akt; C082072:sandaracopimaric acid; D019331:NG-Nitroarginine Methyl Ester