title: DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.
abstract: Morbidity and mortality of prostate cancer (PCa) have increased in recent years worldwide. Currently existing methods for diagnosis and treatment do not make the situation improve, especially for hormone refractory prostate cancer (HRPC). The lack of molecular probes for PCa hindered the early diagnosis of metastasis and accurate staging for PCa. In this work, we have developed a new aptamer probe Wy-5a against PCa cell line PC-3 by cell-SELEX technique. Wy-5a shows high specificity to the target cells with dissociation constants in the nanomolar range, and does not recognize other tested PCa cell lines and other tested tumor cell lines. The staining of clinical tissue sections with fluorescent dye labeled Wy-5a shows that sections from high risk group with metastasis exhibited stronger fluorescence and sections from Benign Prostatic Hyperplasia (BPH) did not exhibit notable fluorescence, which suggests that aptamer Wy-5a may bind to protein related to the progression of PCa. The high affinity and specificity of Wy-5a makes this aptamer hold potential for application in diagnosis and target therapy of PCa.
authors: Department of Urology, the third affiliated hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
authors: Yuanyuan
authors: Y
authors: Wang
authors: Department of Urology, the third affiliated hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
authors: Yun
authors: Y
authors: Luo
authors: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
authors: Tao
authors: T
authors: Bing
authors: Department of Urology, the third affiliated hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
authors: Zheng
authors: Z
authors: Chen
authors: Department of Urology, the third affiliated hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
authors: Minhua
authors: M
authors: Lu
authors: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
authors: Nan
authors: N
authors: Zhang
authors: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
authors: Dihua
authors: D
authors: Shangguan
authors: Department of Urology, the third affiliated hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
authors: Xin
authors: X
authors: Gao
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide