title: The mitochondrial complex I of trypanosomatids--an overview of current knowledge.
abstract: The contribution of trypanosomatid mitochondrial complex I for energy transduction has long been debated. Herein, we summarize current knowledge on the composition and relevance of this enzyme. Bioinformatic and proteomic analyses allowed the identification of many conserved and trypanosomatid-specific subunits of NADH:ubiquinone oxidoreductase, revealing a multifunctional enzyme capable of performing bioenergetic activities and possibly, also of functioning in fatty acid metabolism. A multimeric structure organized in 5 domains of more than 2 MDa is predicted, in contrast to the 1 MDa described for mammalian complex I. The relevance of mitochondrial complex I within the Trypanosomatidae family is quite diverse with its NADH oxidation activity being dispensable for both procyclic and bloodstream Trypanosoma brucei, whereas in Phytomonas serpens the enzyme is the only respiratory complex able to sustain membrane potential. Aside from complex I, trypanosomatid mitochondria contain a type II NADH dehydrogenase and a NADH-dependent fumarate reductase as alternative electron entry points into the respiratory chain and thus, some trypanosomatids may have bypassed the need for complex I. The involvement of each of these enzymes in the maintenance of the mitochondrial redox balance in trypanosomatids is still an open question and requires further investigation.
authors: IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180, Porto, Portugal, mduarte@ibmc.up.pt.
authors: Margarida
authors: M
authors: Duarte
authors: Ana M
authors: AM
authors: Tomás
mesh_terms: D042967:Electron Transport Complex I / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D015800:Protozoan Proteins / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000201:enzymology*
chemical_list: D024101:Mitochondrial Proteins; D015800:Protozoan Proteins; D009243:NAD; C033727:fumarate reductase complex; D013385:Succinate Dehydrogenase; D042967:Electron Transport Complex I