title: Generation of CD44 gene-deficient mouse derived induced pluripotent stem cells: CD44 gene-deficient iPSCs.
abstract: Induced pluripotent stem cells (iPSCs) show good promise for the treatment of defects caused by numerous genetic diseases. Herein, we successfully generated CD44 gene-deficient iPSCs using Oct4, Sox2, Klf4, and vitamin C. The generated iPSCs displayed a characteristic morphology similar to the well-characterized embryonic stem cells. Alkaline phosphatase, cell surface (SSEA1, NANOG, and OCT4), and pluripotency markers were expressed at high levels in these cells. The iPSCs formed teratomas in vivo and supported full-term development of constructed porcine embryos by inter-species nuclear transplantation. Importantly, incubation with trichostatin A increased the efficiency of iPSCs generation by increasing the histone acetylation levels. Moreover, more iPSCs colonies appeared following cell passaging during colony picking, thus increasing the effectiveness of iPSCs selection. Thus, our work provides essential stem cell materials for the treatment of genetic diseases and proposes a novel strategy to enhance the efficiency of induced reprogramming.
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Higher Education Mega Center, Guangzhou, 510006, People's Republic of China, gactu@163.com.
authors: Zhenwei
authors: Z
authors: Song
authors: Qianqian
authors: Q
authors: Ji
authors: Haijing
authors: H
authors: Zhao
authors: Yu
authors: Y
authors: Nie
authors: Zuyong
authors: Z
authors: He
authors: Yaosheng
authors: Y
authors: Chen
authors: Peiqing
authors: P
authors: Cong
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology; D018960:Hyaluronan Receptors / Q000235:genetics*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D000090062:Kruppel-Like Factor 4; D051379:Mice; D053652:Nuclear Transfer Techniques; D013552:Swine
chemical_list: C476399:Cd44 protein, mouse; D018960:Hyaluronan Receptors; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4