title: Genome-wide screen for serum microRNA expression profile in mfat-1 transgenic mice.
abstract: n-3 Polyunsaturated fatty acids (n-3 PUFAs) contribute to preventing many types of diseases, including cancer; however, a high n-6 polyunsaturated fatty acids (n-6 PUFAs) intake in modern diets has the opposite effect. Previously, we developed a transgenic mouse model that expresses a gene, fat-1, encoding an n-3 fatty acid desaturase, which converts n-6 PUFAs to n-3 PUFAs in vivo. MicroRNAs (miRNAs) in serum are stable, reproducible, and consistent among individuals of the same species and serve as potential biomarkers for the detection of cancers and other diseases. Employing illumina sequencing, we analyzed all the serum miRNAs in wild-type and mfat-1 transgenic mice. Using quantitative real-time PCR (RT-qPCR), we identified 12 miRNAs that were highly expressed in mfat-1 mice. Pathway analysis of targets regulated by these miRNAs revealed a significant number of genes involved in the development of cancer, including phosphatidylinositol 3-kinase (PI3K), mitogen-activated protein kinases (MAPK), and mammalian target of rapamycin (mTOR), which suggested a relationship between n-3 PUFAs and cancer prevention.
authors: State Key Laboratory of Reproductive Medicine, Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu, 210029, China.
authors: Zhuo
authors: Z
authors: Gao
authors: Yan
authors: Y
authors: Wang
authors: Zijian
authors: Z
authors: Ren
authors: Qiankun
authors: Q
authors: Li
authors: Ying
authors: Y
authors: Wang
authors: Yifan
authors: Y
authors: Dai
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics*; D043371:Fatty Acids, Omega-6 / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000097:blood*; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D015820:Cadherins; D043371:Fatty Acids, Omega-6; D035683:MicroRNAs; C502406:fat1 protein, mouse