title: Hemocompatibility and cytocompatibility of the hirudin-modified silk fibroin.
abstract: Hirudin (Hir), a thrombin direct inhibitor, was used to modify a polyethylene glycol diglycidyl ether (PEG-DE) crosslinked regenerated silk fibroin (SF) material to improve hemocompatibility. Hemolysis characteristics, platelet adhesion, platelet activity, and plasma recalcification time were investigated using absorption spectrometry, scanning electron microscopy, MTT analysis, and the time counting method. Hirudin could be grafted evenly to the silk fibroin, and the modified material was resistant to hemolysis at ratios of less than 0.5%. Scanning electron microscopy and MTT results showed that platelet adhesion and aggregation activity decreased after modificaton with trace amounts of hirudin, compared with PEG-DE crosslinked and ethanol-treated silk fibroin film. Plasma recalcification of PEG-DE crosslinked silk fibroin film was slower than with ethanol-treated material, and this increased slightly after hirudin modification. Furthermore, L929, HAVSMC, and HUVEC cells adhered to the modified material, grew well, and possessed high proliferation activity on SF/Hir blend films. This study suggests that hirudin could improve the anticoagulation properties of regenerated silk fibroin materials.
authors: National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, People's Republic of China, College of Textile and Clothing Engineering, Soochow University, Suzhou, People's Republic of China; College of Light Industry and Textile, Qiqihar University, Qiqihar, People's Republic of China.
authors: Dan
authors: D
authors: Sun
authors: Yunxia
authors: Y
authors: Hao
authors: Gaoqiang
authors: G
authors: Yang
authors: Jiannan
authors: J
authors: Wang
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D004853:Epoxy Resins / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D005349:Fibroins / Q000187:drug effects / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006629:Hirudins / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects; D010949:Plasma / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects; D011138:Polytetrafluoroethylene / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D004853:Epoxy Resins; D006629:Hirudins; C482288:fibroin, silkworm; C035364:Quetol 651; D011138:Polytetrafluoroethylene; D004247:DNA; D005349:Fibroins
keywords: PEG-DE; cytocompatibility; hemocompatibility; hirudin; silk fibroin