title: Light sheet microscopy.
abstract: This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an alternative to confocal microscopy due to several superior features such as high-speed full-frame acquisition, minimal phototoxicity, and multiview sample rotation. Based on our experience over the last 10 years, we summarize the key concepts in light sheet microscopy, typical implementations, and successful applications. In particular, sample mounting for long time-lapse imaging and the resulting challenges in data processing are discussed in detail.
authors: Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany.
authors: Michael
authors: M
authors: Weber
authors: Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany.
authors: Michaela
authors: M
authors: Mickoleit
authors: Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany.
authors: Jan
authors: J
authors: Huisken
mesh_terms: D000818:Animals; D006801:Humans; D007089:Image Enhancement; D021621:Imaging, Three-Dimensional / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D059008:Time-Lapse Imaging / Q000379:methods
keywords: Developmental biology; Fluorescence microscopy; Image processing; Light sheet microscopy; Mounting; Selective plane illumination microscopy; Three-dimensional imaging; Ultramicroscopy; Zebrafish