title: Influence of Egr-1 in cardiac tissue-derived mesenchymal stem cells in response to glucose variations.
abstract: Mesenchymal stem cells (MSCs) represent a promising cell population for cell therapy and regenerative medicine applications. However, how variations in glucose are perceived by MSC pool is still unclear. Since, glucose metabolism is cell type and tissue dependent, this must be considered when MSCs are derived from alternative sources such as the heart. The zinc finger transcription factor Egr-1 is an important early response gene, likely to play a key role in the glucose-induced response. Our aim was to investigate how short-term changes in in vitro glucose concentrations affect multipotent cardiac tissue-derived MSCs (cMSCs) in a mouse model of Egr-1 KO (Egr-1(-/-)). Results showed that loss of Egr-1 does not significantly influence cMSC proliferation. In contrast, responses to glucose variations were observed in wt but not in Egr-1(-/-) cMSCs by clonogenic assay. Phenotype analysis by RT-PCR showed that cMSCs Egr-1(-/-) lost the ability to regulate the glucose transporters GLUT-1 and GLUT-4 and, as expected, the Egr-1 target genes VEGF, TGF β -1, and p300. Acetylated protein levels of H3 histone were impaired in Egr-1(-/-) compared to wt cMSCs. We propose that Egr-1 acts as immediate glucose biological sensor in cMSCs after a short period of stimuli, likely inducing epigenetic modifications.
authors: Department of Medical-Surgical Sciences and Biotechnologies, Faculty of Pharmacy and Medicine, University "Sapienza", Corso della Repubblica 79, 04100 Latina, Italy.
authors: Daniela
authors: 0000-0003-2397-1718
authors: D
authors: Bastianelli
authors: Department of Medical-Surgical Sciences and Biotechnologies, Faculty of Pharmacy and Medicine, University "Sapienza", Corso della Repubblica 79, 04100 Latina, Italy ; Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161 Rome, Italy.
authors: Camilla
authors: C
authors: Siciliano
authors: Department of Medical-Surgical Sciences and Biotechnologies, Faculty of Pharmacy and Medicine, University "Sapienza", Corso della Repubblica 79, 04100 Latina, Italy.
authors: Rosa
authors: 0000-0001-6749-4496
authors: R
authors: Puca
authors: Department of Medical-Surgical Sciences and Biotechnologies, Faculty of Pharmacy and Medicine, University "Sapienza", Corso della Repubblica 79, 04100 Latina, Italy.
authors: Andrea
authors: 0000-0002-3403-1492
authors: A
authors: Coccia
authors: School of Medicine, Aston University, Aston Triangle, Birmingham B4 7ET, UK.
authors: Colin
authors: 0000-0002-0274-819X
authors: C
authors: Murdoch
authors: Department of Medical-Surgical Sciences and Biotechnologies, Faculty of Pharmacy and Medicine, University "Sapienza", Corso della Repubblica 79, 04100 Latina, Italy.
authors: Antonella
authors: A
authors: Bordin
authors: Department of Medical-Surgical Sciences and Biotechnologies, Faculty of Pharmacy and Medicine, University "Sapienza", Corso della Repubblica 79, 04100 Latina, Italy.
authors: Giorgio
authors: G
authors: Mangino
authors: Laboratory of Vascular Biology and Regenerative Medicine, Centro Cardiologico Monzino, IRCCS, Via C. Parea 4, 20138 Milan, Italy ; Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Via Francesco Sforza 35, 20122 Milan, Italy.
authors: Giulio
authors: G
authors: Pompilio
authors: Department of Medical-Surgical Sciences and Biotechnologies, Faculty of Pharmacy and Medicine, University "Sapienza", Corso della Repubblica 79, 04100 Latina, Italy.
authors: Antonella
authors: A
authors: Calogero
authors: Department of Medical-Surgical Sciences and Biotechnologies, Faculty of Pharmacy and Medicine, University "Sapienza", Corso della Repubblica 79, 04100 Latina, Italy.
authors: Elena
authors: E
authors: De Falco
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D051766:Early Growth Response Protein 1 / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D013549:Sweetening Agents / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D013549:Sweetening Agents; D005947:Glucose