title: Surface-modified P(HEMA-co-MAA) nanogel carriers for oral vaccine delivery: design, characterization, and in vitro targeting evaluation.
abstract: Oral drug delivery is a route of choice for vaccine administration because of its noninvasive nature and thus efforts have focused on efficient delivery of vaccine antigens to mucosal sites. An effective oral vaccine delivery system must protect the antigen from degradation upon mucosal delivery, penetrate mucosal barriers, and control the release of the antigen and costimulatory and immunomodulatory agents to specific immune cells (i.e., APCs). In this paper, mannan-modified pH-responsive P(HEMA-co-MAA) nanogels were synthesized and assessed as carriers for oral vaccination. The nanogels showed pH-sensitive properties, entrapping and protecting the loaded cargo at low pH values, and triggered protein release after switching to intestinal pH values. Surface decoration with mannan as carbohydrate moieties resulted in enhanced internalization by macrophages as well as increasing the expression of relevant costimulatory molecules. These findings indicate that mannan-modified P(HEMA-co-MAA) nanogels are a promising approach to a more efficacious oral vaccination regimen.
authors: Department of Biomedical Engineering, §Department of Chemical Engineering, and ∥Division of Pharmaceutics, University of Texas at Austin , 1 University Station, C0800, Austin, Texas 78712-0238, United States.
authors: Matilde
authors: M
authors: Durán-Lobato
authors: Brenda
authors: B
authors: Carrillo-Conde
authors: Yasmine
authors: Y
authors: Khairandish
authors: Nicholas A
authors: NA
authors: Peppas
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D005782:Gels; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008351:Mannans / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D049329:Nanostructures / Q000737:chemistry; D013499:Surface Properties; D014612:Vaccines / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D005782:Gels; D008351:Mannans; D008689:Methacrylates; D014612:Vaccines; C005044:hydroxyethyl methacrylate