title: Multiresidue analysis of 22 sulfonamides and their metabolites in animal tissues using quick, easy, cheap, effective, rugged, and safe extraction and high resolution mass spectrometry (hybrid linear ion trap-Orbitrap).
abstract: A new high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) method was developed for a simultaneous multi-residue analysis of 22 sulfonamides (SAs) and their metabolites in edible animal (pig, beef, sheep and chicken) tissues. Sample preparation was optimized on the basis of the "QuEChERS" protocol. The analytes were identified using their LC retention times and accurate mass; the identification was further confirmed by multi-stage high mass accuracy (<5ppm) mass spectrometry. The performance of the method was evaluated according to the EU guidelines for the validation of screening methods for the analysis of veterinary drugs residues. Acceptable values were obtained for: linearity (R(2)<0.99), limit of detection (LOD, 3-26μg/kg), limit of quantification (LOQ, 11-88μg/kg), accuracy (recovery 88-112%), intra- and inter-day precision 1-14 and 1-17%, respectively, decision limit (CCα) and detection capability (CCβ) around the maximum residue limits (MRL) of SAs (100μg/kg). The method was validated by analysis of a reference material FAPAS-02188 "Pig kidney" with ǀ Z-scoreǀ<0.63. The method was applied to various matrices (kidney, liver, muscle) originated from pig, beef, sheep, and chicken) allowing the simultaneous quantification of target sulfonamides at concentration levels above the MRL/2 and the identification of untargeted compounds such as N(4)-acetyl metabolites using multi-stage high mass accuracy mass spectrometry.
authors: CNRSL, Lebanese Atomic Energy Commission (LAEC), Laboratory for Analysis of Organic Compound (LAOC), Beirut, Lebanon; CNRS/UPPA, Laboratory of Bio-Inorganic Analytical and Environmental Chemistry (LCABIE), UMR5254, Hélioparc, 2, Av. President Angot, 64053 Pau, France.
authors: H
authors: H
authors: Abdallah
authors: CNRS/UPPA, Laboratory of Bio-Inorganic Analytical and Environmental Chemistry (LCABIE), UMR5254, Hélioparc, 2, Av. President Angot, 64053 Pau, France.
authors: C
authors: C
authors: Arnaudguilhem
authors: CNRSL, Lebanese Atomic Energy Commission (LAEC), Laboratory for Analysis of Organic Compound (LAOC), Beirut, Lebanon; Laboratory of Analysis of Organic Compounds (509), Faculty of Sciences I, Lebanese University, Hadath, Beirut, Lebanon. Electronic address: fjaber@cnrs.edu.lb.
authors: F
authors: F
authors: Jaber
authors: CNRS/UPPA, Laboratory of Bio-Inorganic Analytical and Environmental Chemistry (LCABIE), UMR5254, Hélioparc, 2, Av. President Angot, 64053 Pau, France; Department of Chemistry, Warsaw University of Technology, ul. Noakowskiego 3, 00-664 Warsaw, Poland.
authors: R
authors: R
authors: Lobinski
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D015203:Reproducibility of Results; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D013449:Sulfonamides
keywords: Animal tissues; Metabolites; Orbitrap; QuEChERS; Sulfonamide antibiotics; Validation