title: Identification of early diagnostic antigens from Spirometra erinaceieuropaei sparganum soluble proteins using immunoproteomics.
abstract: In order to identify early specific diagnostic antigens of Spirometra erinaceieuropaei (syn. S. erinacei or S. mansoni) sparganum, soluble proteins were analyzed by two-dimensional electrophoresis (2-DE) and western blotting probed with immune sera from infected mice at 14 days post-infection. From a total of approximately 462 proteins spots mainly distributed in pI range of 5-6.6 and with molecular mass of 25-48 kDa, 6 immuno-reactive protein spots with molecular mass of 31.8-38.3 kDa were characterized by MALDI-TOF/TOF-MS. Three proteins were identified as S. erinaceieuropaei cysteine protease, Toxoplasma gondii hypothetical protein and Pecten spp actin, while the remaining were unidentified. The cysteine protease from S. erinaceieuropaei soluble proteins recognized by early infection sera might be developed as diagnostic reagent for early detection of sparganosis.
authors: Dan Dan
authors: DD
authors: Hu
authors: Jing
authors: J
authors: Cui
authors: Di
authors: D
authors: Xiao
authors: Li
authors: L
authors: Wang
authors: Li Na
authors: LN
authors: Liu
authors: Ruo Dan
authors: RD
authors: Liu
authors: Jian Zhong
authors: JZ
authors: Zhang
authors: Zhong Quan
authors: ZQ
authors: Wang
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D015801:Helminth Proteins / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007162:Immunoproteins / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D040901:Proteomics / Q000379:methods*; D013031:Sparganosis / Q000175:diagnosis* / Q000276:immunology*; D013032:Sparganum / Q000276:immunology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera; D007162:Immunoproteins