title: Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells.
abstract: A new setup for direct microspectroscopic monitoring of singlet oxygen ((1)O2) has been developed in our laboratory using a novel near-infrared sensitive InGaAs 2D-array detector. An imaging spectrograph has been inserted in front of the 2D-array detector, which allows us to acquire spectral images where one dimension is spatial and the other is spectral. The work presents a detailed examination of sensitivity and noise characteristics of the setup and its ability to detect (1)O2. The (1)O2 phosphorescence-based images and near-infrared luminescence spectral images recorded from single TMPyP-containing fibroblast cells reflecting spectral changes during irradiation are demonstrated. The introduction of spectral images addresses the issue of a potential spectral overlap of (1)O2 phosphorescence with near-infrared-extended luminescence of the photosensitizer and provides a powerful tool for distinguishing and separating them, which can be applied to any photosensitizer manifesting near-infrared luminescence.
authors: Charles University in Prague, Faculty of Mathematics and Physics, Department of Chemical Physics and Optics, Ke Karlovu 3, 121 16 Praha 2, The Czech Republic. marasolc@centrum.cz.
authors: Marek
authors: M
authors: Scholz
authors: Roman
authors: R
authors: Dědic
authors: Jan
authors: J
authors: Valenta
authors: Thomas
authors: T
authors: Breitenbach
authors: Jan
authors: J
authors: Hála
mesh_terms: D016475:3T3 Cells; D000818:Animals; D003199:Computer Systems; D005347:Fibroblasts / Q000378:metabolism; D049449:Luminescence; D051379:Mice; D015591:Microspectrophotometry / Q000295:instrumentation / Q000379:methods*; D055668:Photochemical Processes; D017319:Photosensitizing Agents; D011166:Porphyrins; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*; D026082:Singlet Oxygen / Q000378:metabolism*
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins; D026082:Singlet Oxygen; C021096:tetra(4-N-methylpyridyl)porphine