title: Microinjection free delivery of miRNA inhibitor into zygotes.
abstract: The development of gene delivery systems into embryos is challenging due to technical difficulties, delivery efficiency and toxicity. Here, we developed an organic compound (VisuFect)-mediated gene delivery system for zygotes. The VisuFect, which is hydrophilic and Cy5.5-labeled, was conjugated with poly(A) oligo (VFA). The VFA into CHO cells showed clathrin-mediated internalization and no toxicity. The VFA successfully penetrated through the zona pellucida of fertilized eggs of various species including pigs, zebrafish, drosophilas and mice. The experiment with VisuFect-mediated delivery of the miR34c inhibitor showed similar results with direct microinjection of the miR34c inhibitor by suppressing the development of zygotes up to the blastocyst stage. Noticeable features of the VisuFect will provide great benefits for further studies on gene function in sperms and embryos.
authors: 1] Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea [2].
authors: Jin Young
authors: JY
authors: Joo
authors: 1] Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea [2].
authors: Jonghwan
authors: J
authors: Lee
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea.
authors: Hae Young
authors: HY
authors: Ko
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea.
authors: Yong Seung
authors: YS
authors: Lee
authors: College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors: Do-Hwan
authors: DH
authors: Lim
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea.
authors: Eun-Young
authors: EY
authors: Kim
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea.
authors: Sujeong
authors: S
authors: Cho
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea.
authors: Ki-Sung
authors: KS
authors: Hong
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea.
authors: Jung Jae
authors: JJ
authors: Ko
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea.
authors: Suman
authors: S
authors: Lee
authors: College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors: Young Sik
authors: YS
authors: Lee
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea.
authors: Youngsok
authors: Y
authors: Choi
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea.
authors: Kyung-Ah
authors: KA
authors: Lee
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul 130-081, Republic of Korea.
authors: Soonhag
authors: S
authors: Kim
mesh_terms: D000818:Animals; D016466:CHO Cells; D002232:Carbocyanines / Q000737:chemistry; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004330:Drosophila; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D008845:Microinjections; D018613:Microscopy, Confocal; D009841:Oligonucleotides / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics*; D009930:Organic Chemicals / Q000737:chemistry; D011061:Poly A / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D013552:Swine; D059008:Time-Lapse Imaging / Q000379:methods; D014162:Transfection / Q000379:methods*; D015027:Zebrafish; D015053:Zygote / Q000254:growth & development / Q000378:metabolism*
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D035683:MicroRNAs; D009841:Oligonucleotides; D009930:Organic Chemicals; D011061:Poly A