title: Cloning and expression of floral organ development-related genes in herbaceous peony (Paeonia lactiflora Pall.).
abstract: Herbaceous peony (Paeonia lactiflora Pall.) is an important ornamental plant that has different flower types. However, the molecular mechanism underlying its floral organ development has not been fully investigated. This study isolated six floral organ development-related genes in P. lactiflora, namely, APETALA1 (PlAP1), APETALA2 (PlAP2), APETALA3-1 (PlAP3-1), APETALA3-2 (PlAP3-2), PISTILLATA (PlPI) and SEPALLATA3 (PlSEP3). The expression patterns of these genes were also investigated in the three cultivars 'Hangshao', 'Xiangyangqihua' and 'Dafugui'. Furthermore, gene expression during floral development was also analyzed in different organs. The results showed that PlAP1 was mainly expressed in the sepals, and PlAP2 was mainly expressed in the carpels and sepals. PlAP3-2 and PlPI had the highest expression levels in the stamens, followed by the petals. The expression levels of PlAP3-1 (from highest to lowest) were in the following order: petals, stamens, carpels and sepals. PlSEP3 was mainly expressed in sepals and carpels. With the depth of stamen petaloidy, the expression levels of PlAP1, PlAP2 and PlSEP3 increased, whereas those of PlAP3-1, PlAP3-2 and PlPI decreased, which showed that PlAP1 mainly determined sepals and petals of P. lactiflora. The PlAP2 not only determined the sepals and petals, and it participated in carpel formation. PlAP3-1, PlAP3-2 and PlPI mainly determined stamens and petals. PlSEP3 determined the identities of sepals and petals. This study would help determine the molecular mechanism underlying floral organ development in P. lactiflora.
authors: Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, People's Republic of China.
authors: Jintao
authors: J
authors: Ge
authors: Daqiu
authors: D
authors: Zhao
authors: Chenxia
authors: C
authors: Han
authors: Jing
authors: J
authors: Wang
authors: Zhaojun
authors: Z
authors: Hao
authors: Jun
authors: J
authors: Tao
mesh_terms: D003001:Cloning, Molecular; D035264:Flowers / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D009928:Organ Specificity / Q000235:genetics; D029596:Paeonia / Q000235:genetics*; D010641:Phenotype