title: Thermoresponsive double network micropillared hydrogels for controlled cell release.
abstract: Thermoresponsive poly(N-isopropylacrylamide) hydrogels (PNIPAAm) have been widely used for controlled cell detachment. In this study, cell release is enhanced via deswelling with a two-pronged approach combining a double network (DN) design and micropatterning. PNIPAAm hydrogels are prepared as DNs comprised of a tightly crosslinked 1st network and a loosely crosslinked 2nd network. Moreover, the PNIPAAm DN hydrogels are prepared as both planar 1.5 mm-thick slabs as well as micropillar arrays (≈200 μm pillar diameter). Compared to the corresponding conventional single network (SN) hydrogels, DN hydrogels exhibit enhanced thermosensitivity and cell release efficiency, particularly for the micropillar arrays.
authors: Department of Biomedical Engineering, Texas A&M University, 5030 Emerging Technologies Building, College Station, TX, 77843, USA.
authors: Ruochong
authors: R
authors: Fei
authors: Huijie
authors: H
authors: Hou
authors: Dany
authors: D
authors: Munoz-Pinto
authors: Arum
authors: A
authors: Han
authors: Mariah S
authors: MS
authors: Hahn
authors: Melissa A
authors: MA
authors: Grunlan
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism; D003692:Delayed-Action Preparations / Q000737:chemistry; D006358:Hot Temperature; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice
chemical_list: D000180:Acrylic Resins; D003692:Delayed-Action Preparations; D020100:Hydrogels; C052970:poly-N-isopropylacrylamide
keywords: cell release; double network hydrogels; micropatterning; photopolymerization; thermoresponsive