title: Effect of stem cell application on Asherman syndrome, an experimental rat model.
abstract: OBJECTIVE
  We evaluate the effect of stem cells to induce endometrial proliferation and angiogenesis on Asherman Syndrome (AS).
  
  
  METHODS
  The experimental study was performed in stemcell research laboratory. Forty Wistar-Albino rats were divided according to groups. In group1 (n = 10) to establish the model; trichloroacetic acid was injected to right uterine horn. Two weeks later, intrauterine synechia was confirmed. In group2 (n = 10), 2 weeks later, 2 × 106 mesenchymal stem cells (MSC) were injected into right uterine horn followed by three intraperitoneal injections of MSCs. In group3 (n = 10), daily oral estrogen was initiated on the second week. In group4 (n = 10), MSC injections and oral estrogen was given together. The amount of fibrosis, vascularisation, inflammation and immunohistochemical staining with vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA) and Ki-67 were evaluated in the uterine tissues.
  
  
  RESULTS
  In all treatment groups; fibrosis decreased but vascularisation and immunhistohemical stainings increased in the experimental side. The amount of fibrosis, vascularisation, Ki-67 and PCNA scores were similar between group2 and 3. In group4, comparing to group2, less fibrosis but more Ki-67, PCNA and VEGF staining was observed.
  
  
  CONCLUSIONS
  Stem cells, when added to estrogen, are a highly effective alternative to induce regeneration of endometrium in Asherman Syndrome therapy.
authors: Kastamonu School of Medicine, Hacettepe University, Süleyman bey sokak 29/10, Maltepe, Ankara, 06420, Turkey, sevtapkilic@gmail.com.
authors: Sevtap
authors: S
authors: Kilic
authors: Beril
authors: B
authors: Yuksel
authors: F
authors: F
authors: Pinarli
authors: A
authors: A
authors: Albayrak
authors: B
authors: B
authors: Boztok
authors: T
authors: T
authors: Delibasi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005355:Fibrosis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D006175:Gynatresia / Q000378:metabolism / Q000473:pathology*; D007124:Immunoenzyme Techniques; D007249:Inflammation / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D018919:Neovascularization, Physiologic; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015415:Biomarkers