title: Cloning, expression, purification, antiserum preparation and its characteristics of the truncated UL6 protein of herpes simplex virus 1.
abstract: The herpes simplex virus 1 (HSV-1) portal protein UL6 is important for HSV-1 replication, however, its precise functions in the virus life cycle are poorly understood. As we known, a relatively important tool for disclosing these functions is the antiserum specifically detecting UL6 in the HSV-1-infected cell. To this end, a recombinant protein consisting of C-terminal 297-676 amino acids of UL6 fused to His-tag was expressed in E. coli and purified from inclusion body by the Ni(2+)-NTA affinity chromatography under denaturing conditions, which was then refolded and used for the preparation of antiserum in rabbit. As results, western blot and immunofluorescence assay showed that this antiserum could specifically detect the purified truncated UL6 as well as native UL6 in the HSV-1 infected cells, indicating that the prepared antiserum could serve as a valuable tool for further exploring the functions of UL6.
authors: Department of Pathogenic Biology and Immunology, Guangzhou Medical University, 195 Dongfeng Xi Road, Yuexiu, Guangzhou, 510182, Guangdong, People's Republic of China.
authors: Meili
authors: M
authors: Li
authors: Wei
authors: W
authors: Cui
authors: Chuncong
authors: C
authors: Mo
authors: Jinlin
authors: J
authors: Wang
authors: Zhiyao
authors: Z
authors: Zhao
authors: Mingsheng
authors: M
authors: Cai
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000276:immunology; D007106:Immune Sera; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D007106:Immune Sera; D011994:Recombinant Proteins; D014764:Viral Proteins; C069185:DNA cleavage and packaging proteins, Herpesvirus