title: Micelle dynamic simulation and physicochemical characterization of biorelevant media to reflect gastrointestinal environment in fasted and fed states.
abstract: The characterization of biorelevant media simulating the upper part of the gastrointestinal tract in the fasted and fed states was investigated by classical determination of physicochemical parameters such as pH, osmolality, surface tension and results were compared to in vivo physiological data. Incorporation of fatty material, in order to better simulate the influence of high fat meal was also performed. Stability and characterization of this medium was studied and compared to classical FeSSIF. Micelle characterization and computer dynamic simulation were performed in order to understand the interaction between lecithin and taurocholate and possible interactions between mixed micelle and drugs. The addition of NaTc, lecithin, and/or fatty materials has no influence on pH and osmolality, whereas the presence of fatty material modifies the surface tension. Values of FaSSIF and FeSSIF are in accordance with in vivo parameters and the presence of micelles can simulate the gastrointestinal environment. Modelization of micelles by computer simulation led to a model of mixed micelles in which structures of NaTc interact either by their hydrophilic or hydrophobic phase to give a bilayer stable model in which the lecithin molecule can insert its long carbon chain. The micelle structure is stable and can enhance dissolution of hydrophobic molecules by hydrophobic interaction with the numerous hydrophobic spaces available in the multilayer hydrophilic/hydrophobic layer.
authors: Clermont-Université, Université d'Auvergne, EA4678, Conception ingénierie et développement de l'aliment et du médicament, Clermont-Ferrand, France.
authors: XiaoYu
authors: X
authors: Xie
authors: Clermont-Université, Université d'Auvergne, EA4678, Conception ingénierie et développement de l'aliment et du médicament, Clermont-Ferrand, France.
authors: Jean-Michel
authors: JM
authors: Cardot
authors: Clermont-Université, Université d'Auvergne, EA4678, Conception ingénierie et développement de l'aliment et du médicament, Clermont-Ferrand, France.
authors: Ghislain
authors: G
authors: Garrait
authors: Clermont Université, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, Aubière, France.
authors: Vincent
authors: V
authors: Thery
authors: Clermont-Université, Université d'Auvergne, EA4678, Conception ingénierie et développement de l'aliment et du médicament, Clermont-Ferrand, France.
authors: Mohamed
authors: M
authors: El-Hajji
authors: Clermont-Université, Université d'Auvergne, EA4678, Conception ingénierie et développement de l'aliment et du médicament, Clermont-Ferrand, France. Electronic address: eric.beyssac@udamail.fr.
authors: Eric
authors: E
authors: Beyssac
mesh_terms: D003198:Computer Simulation; D005215:Fasting; D041981:Gastrointestinal Tract / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D013500:Surface Tension
chemical_list: D008823:Micelles
keywords: Biorelevant media; Dissolution; Drug/micelle interaction; Dynamic simulation; Fatty material; Micelle