title: Modulation of dendritic cell function by Trichomonas vaginalis-derived secretory products.
abstract: Trichomoniasis caused by the parasitic protozoan Trichomonas vaginalis is the most common sexually transmitted disease in the world. Dendritic cells are antigen presenting cells that initiate immune responses by directing the activation and differentiation of naïve T cells. In this study, we analyzed the effect of Trichomonas vaginalis-derived Secretory Products on the differentiation and function of dendritic cells. Differentiation of bone marrow-derived dendritic cells in the presence of T. vaginalis-derived Secretory Products resulted in inhibition of lipopolysaccharide-induced maturation of dendritic cells, down-regulation of IL-12, and up-regulation of IL-10. The protein components of T. vaginalis-derived Secretory Products were shown to be responsible for altered function of bone marrow- derived dendritic cells. Chromatin immunoprecipitation assay demonstrated that IL-12 expression was regulated at the chromatin level in T. vaginalis-derived Secretory Productstreated dendritic cells. Our results demonstrated that T. vaginalis- derived Secretory Products modulate the maturation and cytokine production of dendritic cells leading to immune tolerance.
authors: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Min-Ji
authors: MJ
authors: Song
authors: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Jong-Joo
authors: JJ
authors: Lee
authors: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Young Hee
authors: YH
authors: Nam
authors: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Tae-Gyun
authors: TG
authors: Kim
authors: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Youn Wook
authors: YW
authors: Chung
authors: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Mikyoung
authors: M
authors: Kim
authors: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Ye-Eun
authors: YE
authors: Choi
authors: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Myeong Heon
authors: MH
authors: Shin
authors: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Hyoung-Pyo
authors: HP
authors: Kim
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D047369:Chromatin Immunoprecipitation; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D019286:Endopeptidase K / Q000378:metabolism; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D016753:Interleukin-10; D018664:Interleukin-12; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D014246:Trichomonas vaginalis / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000953:Antigens, Protozoan; D008070:Lipopolysaccharides; D016753:Interleukin-10; D018664:Interleukin-12; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D019286:Endopeptidase K