title: The roles of JK-1 (FAM134B) expressions in colorectal cancer.
abstract: The aims of the present study are to investigate the clinicopathological correlations of JK-1(FAM134B) expression and its relationship to carcinogenesis in a colorectal adenoma-adenocarcinoma model. JK-1(FAM134B) protein expression was studied in a colon cancer cell line by Western blot and immunocytochemistry. JK-1(FAM134B) expression profiles at mRNA and protein levels were investigated in cancer tissues from 236 patients with colorectal adenocarcinoma and 32 patients with colorectal adenoma using real-time polymerase chain reaction and immunohistochemistry. The findings were then correlated with the clinicopathological features of these tumours. JK-1(FAM134B) protein was demonstrated in the colon cancer cells by Western blot. The protein was located in the nuclei of the tumour cells at both cellular and tissue levels. In colorectal adenocarcinomas, lower levels of JK-1(FAM134B) protein expression were associated with younger age (p=0.032), larger tumour size (p=0.004), advanced cancer stages (p=0.016) and higher rates of cancer recurrence (p=0.04). Also, lower levels of JK-1(FAM134B) mRNA expression were associated with advanced cancer stages (p=0.02) and presence of lymphovascular invasion (p=0.014). Higher JK-1(FAM134B) mRNA and protein expression levels were identified in adenomas and non-neoplastic mucosae, compared to carcinomas (p=0.005). To conclude, JK-1(FAM134B) mRNA expression and JK1 (FAM134B) protein levels varied with the different stages of progression of colorectal tumours. The expression levels of the gene were associated with clinicopathological features in patients with colorectal adenocarcinoma suggesting that JK-1(FAM134B) gene has roles in controlling some steps in the development of the invasive phenotypes from colorectal adenoma to early staged as well as advanced staged colorectal adenocarcinomas.
authors: Cancer Molecular Pathology, School of Medicine and Griffith Health Institute, Griffith Medical School, Griffith University, Gold Coast Campus, Gold Coast, Queensland 4222, Australia.
authors: Kais
authors: K
authors: Kasem
authors: Cancer Molecular Pathology, School of Medicine and Griffith Health Institute, Griffith Medical School, Griffith University, Gold Coast Campus, Gold Coast, Queensland 4222, Australia.
authors: Vinod
authors: V
authors: Gopalan
authors: Cancer Molecular Pathology, School of Medicine and Griffith Health Institute, Griffith Medical School, Griffith University, Gold Coast Campus, Gold Coast, Queensland 4222, Australia.
authors: Ali
authors: A
authors: Salajegheh
authors: Department of Surgery, Gold Coast University Hospital, Gold Coast, Queensland, Australia.
authors: Cu-Tai
authors: CT
authors: Lu
authors: Cancer Molecular Pathology, School of Medicine and Griffith Health Institute, Griffith Medical School, Griffith University, Gold Coast Campus, Gold Coast, Queensland 4222, Australia.
authors: Robert A
authors: RA
authors: Smith
authors: Cancer Molecular Pathology, School of Medicine and Griffith Health Institute, Griffith Medical School, Griffith University, Gold Coast Campus, Gold Coast, Queensland 4222, Australia. Electronic address: a.lam@griffith.edu.au.
authors: Alfred K-Y
authors: AK
authors: Lam
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000236:Adenoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D015331:Cohort Studies; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions; D015996:Survival Rate; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C522691:RETREG1 protein, human; D012333:RNA, Messenger
keywords: Adenocarcinoma; Adenoma; Colorectal cancer; Expression; FAM134B; JK-1