title: An acyl-SAM analog as an affinity ligand for identifying quorum sensing signal synthases.
abstract: N-Acylhomoserine lactones (AHLs) are quorum sensing signals produced by Gram-negative bacteria. We here report the affinity purification of AHL synthases using beads conjugated with an enzyme inhibitor, which was designed based on the catalytic intermediate acyl-SAM.
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. kai@biochem.osakafu-u.ac.jp.
authors: Kenji
authors: K
authors: Kai
authors: Hiroki
authors: H
authors: Fujii
authors: Rui
authors: R
authors: Ikenaka
authors: Mitsugu
authors: M
authors: Akagawa
authors: Hideo
authors: H
authors: Hayashi
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology; D007700:Kinetics; D008025:Ligases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D012436:S-Adenosylmethionine / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D054742:Acyl-Butyrolactones; D004791:Enzyme Inhibitors; D012436:S-Adenosylmethionine; D008025:Ligases; C449816:N-acylhomoserine lactone synthase