title: Functional properties of a novel hybrid antimicrobial peptide NS: potent antitumor activity and efficient plasmid delivery.
abstract: Antimicrobial peptides have been widely recognized as potential candidates for treating tumor, especially for defending against multidrug-resistant cells. Previously, based on the structure of substance P, we have designed a novel class of hybrid antimicrobial peptide NS, which possesses potent antimicrobial activity against a broad spectrum of bacterial pathogens. In this study, we evaluated its cytotoxicity to tumor cells and studied the possible mechanism of action. We showed that NS could efficiently kill tumor cells by rapidly disrupting the tumor cell membrane and inhibiting the DNA synthesis. In addition, we also found that NS could efficiently deliver plasmids into cells and exhibit high transfection efficiency after the introduction of a stearyl moiety to its N-terminus, like many reported cell-penetrating peptides. Taken together, this study revealed the potential multiple functions of NS, providing fundamental support for further therapeutic application as potential antitumor agent.
authors: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China; School of Pharmacy, Lanzhou University, Lanzhou, 730000, China.
authors: Yun
authors: Y
authors: Zhang
authors: Jingjing
authors: J
authors: Song
authors: Wei
authors: W
authors: Zhang
authors: Ranran
authors: R
authors: Liang
authors: Yinyun
authors: Y
authors: Ma
authors: Li
authors: L
authors: Zhang
authors: Xiaojin
authors: X
authors: Wei
authors: Jingman
authors: J
authors: Ni
authors: Rui
authors: R
authors: Wang
mesh_terms: D000215:Acylation; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000648:ultrastructure; D019556:COS Cells; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D004261:DNA Replication / Q000187:drug effects; D005260:Female; D015316:Genetic Therapy / Q000379:methods; D005909:Glioblastoma / Q000188:drug therapy* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D010957:Plasmids / Q000378:metabolism* / Q000627:therapeutic use; D021382:Protein Sorting Signals; D021381:Protein Transport; D011994:Recombinant Proteins / Q000494:pharmacology; D013373:Substance P / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D014162:Transfection / Q000379:methods; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D013373:Substance P
keywords: DNA interaction; NS; antitumor activity; membrane disruption; plasmid transfection