title: Critical assessment of human antibody generation in humanized mouse models.
abstract: Immunodeficient mice reconstituted with human hematopoietic stem cells provide a small-animal model for the study of development and function of human hematopoietic cells in vivo. However, in the current models, the immune response, and especially the humoral response by the human immune cells is far from optimal. The B cells found in these mice exhibit an immature and abnormal phenotype correlating with a reduced capacity to produce antigen-specific affinity matured antibodies upon infection or immunization. Herein, we review the current state of knowledge of development, function and antibody production of human B cells and discuss the obstacles for the improvement of these models.
authors: AIMM Therapeutics, Meibergdreef 59, 1105 BA Amsterdam Zuidoost, Netherlands; Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 BA Amsterdam Zuidoost, Netherlands. Electronic address: jvillaudy@aimmtherapeutics.com.
authors: Julien
authors: J
authors: Villaudy
authors: AIMM Therapeutics, Meibergdreef 59, 1105 BA Amsterdam Zuidoost, Netherlands. Electronic address: RSchotte@aimmtherapeutics.com.
authors: Remko
authors: R
authors: Schotte
authors: AXENIS, Institut Pasteur, Centre Francois Jacob, 28, rue du Dr. Roux, 75015 Paris, France. Electronic address: nicolas.legrand@pasteur.fr.
authors: Nicolas
authors: N
authors: Legrand
authors: AIMM Therapeutics, Meibergdreef 59, 1105 BA Amsterdam Zuidoost, Netherlands; Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 BA Amsterdam Zuidoost, Netherlands. Electronic address: hspits@aimmtherapeutics.com.
authors: Hergen
authors: H
authors: Spits
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D006410:Hematopoiesis / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D064593:Heterografts; D006801:Humans; D051379:Mice; D018448:Models, Immunological
chemical_list: D000906:Antibodies
keywords: Antibody; B cell; Human Immune System mouse; Humoral immune response