title: Effect of Alocasia indica tuber extract on reducing hepatotoxicity and liver apoptosis in alcohol intoxicated rats.
abstract: The possible protective role of ethanolic extract of A. indica tuber (EEAIT) in hepatotoxicity and apoptosis of liver caused by alcohol in rats was investigated. Treatment of rats with alcohol (3 g ethanol per kg body weight per day for 15 days intraperitoneally) produced marked elevation of liver biomarkers such as serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (γ-GT), and total bilirubin levels which were reduced by EEAIT in a dose-dependent manner. Furthermore, EEAIT improved antioxidant status (MDA, NO, and GSH) and preserved hepatic cell architecture. Simultaneous supplementation with EEAIT significantly restored hepatic catalase (CAT) and superoxide dismutase (SOD) activity levels towards normal. The studies with biochemical markers were strongly supported by the histopathological evaluation of the liver tissue. EEAIT also attenuated apoptosis and necrosis features of liver cell found in immunohistochemical evaluation. HPLC analysis of the extract showed the presence of three major peaks of which peak 2 (RT: 33.33 min) contains the highest area (%) and UV spectrum analysis identified it as flavonoids. It is therefore suggested that EEAIT can provide a definite protective effect against chronic hepatic injury caused by alcohol in rats, which may mainly be associated with its antioxidative effect.
authors: CSIR-Indian Institute of Chemical Biology, Drug Development and Biotechnology, 4 Raja S. C. Mullick Road, Kolkata 700032, India.
authors: Swagata
authors: 0000-0002-7965-4589
authors: S
authors: Pal
authors: Serampore College, Department of Physiology, Hooghly, West Bengal 712201, India.
authors: Ankita
authors: 0000-0002-7204-1413
authors: A
authors: Bhattacharjee
authors: Serampore College, Department of Physiology, Hooghly, West Bengal 712201, India.
authors: Sandip
authors: 0000-0003-4176-3496
authors: S
authors: Mukherjee
authors: Serampore College, Department of Physiology, Hooghly, West Bengal 712201, India.
authors: Koushik
authors: K
authors: Bhattacharya
authors: CSIR-Indian Institute of Chemical Biology, Drug Development and Biotechnology, 4 Raja S. C. Mullick Road, Kolkata 700032, India.
authors: Soumya
authors: S
authors: Mukherjee
authors: CSIR-Indian Institute of Chemical Biology, Drug Development and Biotechnology, 4 Raja S. C. Mullick Road, Kolkata 700032, India ; Drug Development Diagnostics and Biotechnology Division, CSIR-Indian Institute of Chemical Biology, Government of India, 4 Raja S. C. Mullick Road, Kolkata, West Bengal 700032, India.
authors: Suman
authors: 0000-0002-2034-2900
authors: S
authors: Khowala
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D031055:Alocasia / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001663:Bilirubin / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000009:adverse effects*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007150:Immunohistochemistry; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D017093:Liver Failure / Q000139:chemically induced / Q000188:drug therapy*; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D009569:Nitric Oxide; D000431:Ethanol; D002374:Catalase; D013482:Superoxide Dismutase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D001663:Bilirubin