title: Gene expression profile in delay graft function: inflammatory markers are associated with recipient and donor risk factors.
abstract: BACKGROUND
  Delayed graft function (DGF) remains an important problem after kidney transplantation and reduced long-term graft survival of the transplanted organ. The aim of the present study was to determine if the development of DGF was associated with a specific pattern of inflammatory gene expression in expanded criteria of deceased donor kidney transplantation. Also, we explored the presence of correlations between DGF risk factors and the profile that was found.
  
  
  METHODS
  Seven days after kidney transplant, a cDNA microarray was performed on biopsies of graft from patients with and without DGF. Data was confirmed by real-time PCR. Correlations were performed between inflammatory gene expression and clinical risk factors.
  
  
  RESULTS
  From a total of 84 genes analyzed, 58 genes were upregulated while only 1 gene was downregulated in patients with DGF compared with no DGF (P = 0.01). The most relevant genes fold changes observed was IFNA1, IL-10, IL-1F7, IL-1R1, HMOX-1, and TGF-β. The results were confirmed for IFNA1, IL-1R1, HMOX-1 and TGF-β. A correlation was observed between TGF-β, donor age, and preablation creatinine, but not body mass index (BMI). Also, TGF-β showed an association with recipient age, while IFNA1 correlated with recipient BMI. Furthermore, TGF-β, IFNA1 and HMOX-1 correlated with several posttransplant kidney function markers, such as diuresis, ultrasound Doppler, and glycemia.
  
  
  CONCLUSIONS
  Overall, the present study shows that DGF is associated with inflammatory markers, which are correlated with donor and recipient DGF risk factors.
authors: CEFYBO-School of Medicine, University of Buenos Aires, Paraguay 2155, 16th Floor, C1121ABG Buenos Aires, Argentina.
authors: Diego
authors: D
authors: Guerrieri
authors: Instituto de Nefrología de Buenos Aires, Cabello 3889, C1425APQ Buenos Aires, Argentina.
authors: Luis
authors: 0000-0001-7686-409X
authors: L
authors: Re
authors: Instituto de Nefrología de Buenos Aires, Cabello 3889, C1425APQ Buenos Aires, Argentina.
authors: Jorgelina
authors: J
authors: Petroni
authors: CEFYBO-School of Medicine, University of Buenos Aires, Paraguay 2155, 16th Floor, C1121ABG Buenos Aires, Argentina.
authors: Nella
authors: N
authors: Ambrosi
authors: Instituto de Nefrología de Buenos Aires, Cabello 3889, C1425APQ Buenos Aires, Argentina.
authors: Roxana E
authors: RE
authors: Pilotti
authors: CEFYBO-School of Medicine, University of Buenos Aires, Paraguay 2155, 16th Floor, C1121ABG Buenos Aires, Argentina.
authors: H Eduardo
authors: HE
authors: Chuluyan
authors: Instituto de Nefrología de Buenos Aires, Cabello 3889, C1425APQ Buenos Aires, Argentina.
authors: Domingo
authors: D
authors: Casadei
authors: GADOR S.A., Darwin 429, C1414CUH Buenos Aires, Argentina.
authors: Claudio
authors: 0000-0001-5163-5872
authors: C
authors: Incardona
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D015992:Body Mass Index; D018076:DNA, Complementary / Q000378:metabolism; D051799:Delayed Graft Function / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D051437:Renal Insufficiency / Q000235:genetics / Q000628:therapy; D012307:Risk Factors; D014019:Tissue Donors; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary