title: Durable diagnosis of seminal vesicle and sexual gland diseases using the nano optical sensor thin film Sm-doxycycline complex.
abstract: A new method in which a nano optical sensor for diagnosis of different diseases of seminal vesicle and sexual gland was prepared. The working principle of the method depends on the determination of the fructose concentration in semen of different patients by using nano optical sensor thin film Sm-doxycycline doped in sol-gel matrix. The assay is based on the quenching of the characteristic emission bands of Sm(3+) present in silica doped Sm-doxycycline nanooptode thin film by different fructose concentrations in acetonitrile at λex = 400 nm. This method was optimized for parameters, such as, solvent effect, operational stability, shelf life and interference parameters. Good and reproducible linearity (1 × 10(-9) - 5.0 × 10(-5) mol L(-1)) with a detection limit of 9.0 × 10(-10) mol L(-1) and quantification limit of detection (LOQ) 2.7 × 10(-9) mol L(-1) were obtained. Seminal fructose determination in different patient samples after appropriate dilutions confirmed the reliability of this technique. The method was successfully applied for routine fructose monitoring in human semen samples of different cases such as; obstructive and non-obstructive azoospermia, inflammation of male accessory glands, atrophy of seminal vesicle, congenital vas deferens and retrograde ejaculation.
authors: Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo 11566, Egypt. Electronic address: Mohamed_sam@yahoo.com.
authors: M S
authors: MS
authors: Attia
authors: Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo 11566, Egypt.
authors: A O
authors: AO
authors: Youssef
authors: Laboratory Department-New Al-Kasr-EL-Aini Teaching Hospital-Faculty of Medicine-Cairo University, Cairo 25114, Egypt.
authors: R H
authors: RH
authors: El-Sherif
mesh_terms: D004318:Doxycycline / Q000737:chemistry*; D005832:Genital Diseases, Male / Q000175:diagnosis*; D006801:Humans; D008297:Male; D036103:Nanotechnology / Q000379:methods*; D055096:Optical Devices; D010087:Oxides / Q000737:chemistry*; D012493:Samarium / Q000737:chemistry*; D012669:Seminal Vesicles / Q000737:chemistry*
chemical_list: D010087:Oxides; D012493:Samarium; C120592:samarium oxide; D004318:Doxycycline
keywords: Luminescence intensity; Nano optical sensor thin flim; Quenching; Seminal fructose; Seminal vesicle; Sm-doxycycline