title: Energy-regulated molecules maintain young status in the trophocytes and fat cells of old queen honeybees.
abstract: Queen honeybees (Apis mellifera) have much longer lifespans than worker bees. Energy-regulated molecules in the trophocytes and fat cells of workers during aging have been determined, but are unknown in queen bees. In the present study, energy-regulated molecules were evaluated in the trophocytes and fat cells of young and old queen bees. Adenosine monophosphate-activated protein kinase α2 (AMPK-α2), phosphorylated AMPK-α2 (pAMPK-α2), and cAMP-specific phosphodiesterases activity increased with aging. The pAMPK-α2/AMPK-α2 ratio and AMPK activity; adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) concentrations; the ADP/ATP ratio and the AMP/ATP ratio; the cyclic adenosine monophosphate concentration; forkhead box protein O expression; Silent information regulator T1 (SirT1) expression and activity; and peroxisome proliferator-activated receptor-α (PPAR-α) expression were not significantly different between young and old queen bees. These results show that energy-regulated molecules maintain a youthful status in the trophocytes and fat cells of queen bees during aging. These cells seem to have longevity-promoting mechanisms and may clarify the secret of longevity in queen bees.
authors: Department of Biomedical Sciences, College of Medicine, Chang Gung University, 259, Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, 333, Taiwan, hsu@mail.cgu.edu.tw.
authors: Chin-Yuan
authors: CY
authors: Hsu
authors: Ting-Hui
authors: TH
authors: Hu
mesh_terms: D000263:Adenylate Kinase / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001516:Bees / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004734:Energy Metabolism; D047493:PPAR alpha / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism
chemical_list: D047493:PPAR alpha; D000242:Cyclic AMP; D000263:Adenylate Kinase; D010727:Phosphoric Diester Hydrolases