title: Profiling of mitochondrial proteome in wheat roots.
abstract: Mitochondria are important organelles for cellular respiration within the eukaryotic cell and have many important functions including vitamin synthesis, amino acid metabolism and photorespiration. To investigate the mitochondrial proteome of the roots of wheat seedlings, a systematic and targeted analysis were carried out on the mitochondrial proteome from 15 day-old wheat seedling root material. Mitochondria were isolated by Percoll gradient centrifugation; and extracted proteins were disassociated and analyzed by Tricine SDS-PAGE couple to LTQ-FTICR mass spectrometry. From the isolated the sample, 184 proteins were identified which is composed of 140 proteins as mitochondria and 44 proteins as other subcellular proteins that are predicted by the freeware sub-cellular predictor. The identified proteins in mitochondria were functionally classified into 12 classes using the ProtFun 2.2 servers based on biological processes. Proteins were shown to be involved in amino acid biosynthesis (17.1%), biosynthesis of cofactors (6.4%), cell envelope (11.4%), central intermediary metabolism (10%), energy metabolism (20%), fatty acid metabolism (0.7%), purines and pyrimidines (5.7%), regulatory functions (0.7%), replication and transcription (1.4%), translation (22.1%), transport and binding (1.4%), and unknown (2.8%). These results indicate that many of the protein components present and functions of identifying proteins are common to other profiles of mitochondrial proteins performed to date. These results are provided the extensive and noble clues, to our knowledge, of mitochondrial proteins from wheat roots.
authors: Department of Crop Science, Chungbuk National University, 410 Seongbong-ro, Heungdeok-gu, Cheongju, Chungbuk, 361-763, Korea.
authors: Da-Eun
authors: DE
authors: Kim
authors: Swapan Kumar
authors: SK
authors: Roy
authors: Abu Hena Mostafa
authors: AH
authors: Kamal
authors: Kun
authors: K
authors: Cho
authors: Soo Jeong
authors: SJ
authors: Kwon
authors: Seong-Woo
authors: SW
authors: Cho
authors: Chul-Soo
authors: CS
authors: Park
authors: Jong-Soon
authors: JS
authors: Choi
authors: Setsuko
authors: S
authors: Komatsu
authors: Moon-Soon
authors: MS
authors: Lee
authors: Sun-Hee
authors: SH
authors: Woo
mesh_terms: D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling; D013058:Mass Spectrometry; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
chemical_list: D024101:Mitochondrial Proteins; D010940:Plant Proteins; D020543:Proteome