title: Prostaglandin E2 does not attenuate adrenergic-induced cardiac contractile response.
abstract: Systemic inflammation may contribute to heart failure. PGE2 was recently suggested to mediate inflammation-induced impairment of cardiac function by desensitizing the murine heart to isoprenaline. Given the magnitude of the reported effect and the potential relevance, we sought to reproduce it in the human heart. Human trabeculae were prepared from the right atrial tissue obtained during heart surgery and from the right ventricle of two explanted human failing hearts. Muscle strips were electrically driven and isometric force development was measured. PGE2 was given at a single concentration (0.1 μM). Norepinephrine was used to activate β1-adrenoceptors, epinephrine to activate β2-adrenoceptors in atrial trabeculae. Isoprenaline was used in ventricular tissue. All patients were in sinus rhythm. Murine ventricular strips were used for comparison and stimulated with isoprenaline. The pharmacological activity of the PGE2 batch was confirmed by assessing concentration-dependent vasoconstriction in murine aorta. We used atrial and ventricular trabeculae from humans. Exposure to PGE2 (15 min) did not affect contractility when compared to time-matched controls. PGE2 neither altered the sensitivity or efficacy of β1- or β2-adrenoceptor-mediated stimulation of force in human atrial or in ventricular trabeculae for nonselective β1- or β2-adrenoceptor-stimulation. Surprisingly, PGE2 also did not affect -logEC50 values or maximum catecholamine-stimulated force in ventricular strips from mice, whereas it induced vasoconstriction in aortic rings with an -logEC50 of 5.0 (n = 6). Our data do not support a role for PGE2 in regulating catecholamine inotropy, neither in mice nor in humans.
authors: Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany.
authors: Simon
authors: S
authors: Pecha
authors: Eileen
authors: E
authors: Mudersbach
authors: Klaus-Dieter
authors: KD
authors: Söhren
authors: Samer
authors: S
authors: Hakmi
authors: Hermann
authors: H
authors: Reichenspurner
authors: Thomas
authors: T
authors: Eschenhagen
authors: Torsten
authors: T
authors: Christ
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000368:Aged; D000818:Animals; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4 / Q000502:physiology; D015232:Dinoprostone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009200:Myocardial Contraction / Q000187:drug effects* / Q000502:physiology; D009638:Norepinephrine / Q000494:pharmacology*; D009924:Organ Culture Techniques
chemical_list: D000316:Adrenergic alpha-Agonists; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; D015232:Dinoprostone; D009638:Norepinephrine