title: Sorbitol counteracts high hydrostatic pressure-induced denaturation of inulin fructotransferase.
abstract: Inulin fructotransferase (IFTase), a novel hydrolase for inulin, was exposed to high hydrostatic pressure (HHP) at 400 and 600 MPa for 15 min in the presence or absence of sorbitol. Sorbitol protected the enzyme against HHP-induced activity loss. The relative residual activity increased nearly 3.1- and 3.8-fold in the presence of 3 mol/L sorbitol under 400 MPa and 600 MPa for 15 min, respectively. Circular dichroism results indicated that the original chaotic unfolding conformation of the enzyme under HHP shifted toward more ordered and impact with 3 mol/L sorbitol. Fluorescence and UV spectra results suggested that sorbitol prevented partially the unfolding of the enzyme and stabilized the conformation under high pressure. These results might be attributed to the binding of sorbitol on the surface of IFTase to rearrange and strengthen intra- and intermolecular hydrogen bonds.
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, PR China.
authors: Yungao
authors: Y
authors: Li
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, PR China.
authors: Ming
authors: M
authors: Miao
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, PR China.
authors: Miao
authors: M
authors: Liu
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, PR China. Electronic address: bjiang@jiangnan.edu.cn.
authors: Bo
authors: B
authors: Jiang
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, PR China.
authors: Tao
authors: T
authors: Zhang
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, PR China.
authors: Xiangyin
authors: X
authors: Chen
mesh_terms: D004789:Enzyme Activation / Q000187:drug effects; D006602:Hexosyltransferases / Q000737:chemistry* / Q000378:metabolism*; D006874:Hydrostatic Pressure; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects*; D013012:Sorbitol / Q000494:pharmacology*; D014783:Viscosity / Q000187:drug effects
chemical_list: D013012:Sorbitol; D006602:Hexosyltransferases; C021450:inulin fructotransferase (depolymerizing)
keywords: Conformation; High hydrostatic pressure; Inulin fructotransferase; Sorbitol; Stabilization