title: Enzymatic synthesis and purification of a defined RIG-I ligand.
abstract: Receptor-based nucleic acid sensing constitutes one of the most fundamental mechanisms of our innate immune system to sense viral infection. RIG-I is a cytosolic RNA helicase that senses the presence of 5' triphosphate RNA species, a common feature of many negative strand RNA viruses. We here describe a protocol to enzymatically synthesize and to purify a defined RIG-I ligand that can be used to study RIG-I activation in vitro and in vivo.
authors: Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, Sigmund-Freud-Strasse 25, 53127, Bonn, Germany.
authors: Marion
authors: M
authors: Goldeck
authors: Martin
authors: M
authors: Schlee
authors: Gunther
authors: G
authors: Hartmann
authors: Veit
authors: V
authors: Hornung
mesh_terms: D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism
chemical_list: D012367:RNA, Viral; D053487:DEAD-box RNA Helicases