title: Single-particle tracking for studying nanomaterial dynamics: applications and fundamentals in drug delivery.
abstract: Many macromolecular therapeutics could potentially treat genetic disorders and cancer. They have, however, not yet reached the clinical stage owing to a lack of suitable carriers that can bring the therapeutics from the administration site to the subcellular site in target cells. One of the reasons that is hindering the development of such carriers is the limited knowledge of their transport dynamics and intracellular processing. Single-particle tracking (SPT) microscopy, thanks to its single molecule sensitivity and its broad applicability, has found its entry in the field of drug delivery to get an answer to these questions. This review aims to introduce the fundamentals of SPT to the drug delivery community and highlight the most recent discoveries obtained with SPT in the field of drug delivery.
authors: Laboratory of General Biochemistry & Physical Pharmacy, Ghent University, Ghent, Belgium.
authors: Elisa
authors: E
authors: Zagato
authors: Katrien
authors: K
authors: Forier
authors: Thomas
authors: T
authors: Martens
authors: Kristiaan
authors: K
authors: Neyts
authors: Jo
authors: J
authors: Demeester
authors: Stefaan
authors: S
authors: De Smedt
authors: Katrien
authors: K
authors: Remaut
authors: Kevin
authors: K
authors: Braeckmans
mesh_terms: D000818:Animals; D001692:Biological Transport; D004337:Drug Carriers / Q000008:administration & dosage / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D053758:Nanoparticles / Q000008:administration & dosage / Q000032:analysis*
chemical_list: D004337:Drug Carriers
keywords: diffusion; drug delivery; fluorescence microscopy; nanoparticles; single-particle tracking; transport