title: Immune modulating capability of two exopolysaccharide-producing Bifidobacterium strains in a Wistar rat model.
abstract: Fermented dairy products are the usual carriers for the delivery of probiotics to humans, Bifidobacterium and Lactobacillus being the most frequently used bacteria. In this work, the strains Bifidobacterium animalis subsp. lactis IPLA R1 and Bifidobacterium longum IPLA E44 were tested for their capability to modulate immune response and the insulin-dependent glucose homeostasis using male Wistar rats fed with a standard diet. Three intervention groups were fed daily for 24 days with 10% skimmed milk, or with 10(9) cfu of the corresponding strain suspended in the same vehicle. A significant increase of the suppressor-regulatory TGF- β cytokine occurred with both strains in comparison with a control (no intervention) group of rats; the highest levels were reached in rats fed IPLA R1. This strain presented an immune protective profile, as it was able to reduce the production of the proinflammatory IL-6. Moreover, phosphorylated Akt kinase decreased in gastroctemius muscle of rats fed the strain IPLA R1, without affecting the glucose, insulin, and HOMA index in blood, or levels of Glut-4 located in the membrane of muscle and adipose tissue cells. Therefore, the strain B. animalis subsp. lactis IPLA R1 is a probiotic candidate to be tested in mild grade inflammation animal models.
authors: Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias-Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, Villaviciosa, 33300 Asturias, Spain.
authors: Nuria
authors: 0000-0003-1435-7628
authors: N
authors: Salazar
authors: Department of Functional Biology, Immunology Area, University of Oviedo, C/Julián Clavería s/n, Oviedo, 33006 Asturias, Spain.
authors: Patricia
authors: P
authors: López
authors: Department of Functional Biology, Physiology Area, University of Oviedo, C/Julián Clavería s/n, Oviedo, 33006 Asturias, Spain.
authors: Pablo
authors: 0000-0001-7062-1855
authors: P
authors: Garrido
authors: Department of Functional Biology, Physiology Area, University of Oviedo, C/Julián Clavería s/n, Oviedo, 33006 Asturias, Spain.
authors: Javier
authors: J
authors: Moran
authors: Department of Functional Biology, Physiology Area, University of Oviedo, C/Julián Clavería s/n, Oviedo, 33006 Asturias, Spain.
authors: Estefanía
authors: E
authors: Cabello
authors: Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias-Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, Villaviciosa, 33300 Asturias, Spain.
authors: Miguel
authors: 0000-0002-0192-901X
authors: M
authors: Gueimonde
authors: Department of Functional Biology, Immunology Area, University of Oviedo, C/Julián Clavería s/n, Oviedo, 33006 Asturias, Spain.
authors: Ana
authors: A
authors: Suárez
authors: Department of Functional Biology, Physiology Area, University of Oviedo, C/Julián Clavería s/n, Oviedo, 33006 Asturias, Spain.
authors: Celestino
authors: C
authors: González
authors: Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias-Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, Villaviciosa, 33300 Asturias, Spain.
authors: Clara G
authors: CG
authors: de los Reyes-Gavilán
authors: Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias-Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, Villaviciosa, 33300 Asturias, Spain.
authors: Patricia
authors: 0000-0001-6158-9320
authors: P
authors: Ruas-Madiedo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001644:Bifidobacterium / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D005285:Fermentation; D006801:Humans; D007155:Immunologic Factors / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008892:Milk / Q000382:microbiology*; D023421:Models, Animal; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D019936:Probiotics / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D015850:Interleukin-6; D011135:Polysaccharides, Bacterial