title: Prospective evaluation of whole genome microRNA expression profiling in childhood acute lymphoblastic leukemia.
abstract: Dysregulation of microRNA (miRNA) expression contributes to the pathogenesis of several clinical conditions. The aim of this study is to evaluate the associations between miRNAs and childhood acute lymphoblastic leukemia (ALL) to discover their role in the course of the disease. Forty-three children with ALL and 14 age-matched healthy controls were included in the study. MicroRNA microarray expression profiling was used for peripheral blood and bone marrow samples. Aberrant miRNA expressions associated with the diagnosis and outcome were prospectively evaluated. Confirmation analysis was performed by real time RT-PCR. miR-128, miR-146a, miR-155, miR-181a, and miR-195 were significantly dysregulated in ALL patients at day 0. Following a six-month treatment period, the change in miRNA levels was determined by real time RT-PCR and expression of miR-146a, miR-155, miR-181a, and miR-195 significantly decreased. To conclude, these miRNAs not only may be used as biomarkers in diagnosis of ALL and monitoring the disease but also provide new insights into the potential roles of them in leukemogenesis.
authors: Department of Pediatrics, Faculty of Medicine, Ege University, 35100 Izmir, Turkey.
authors: Muhterem
authors: M
authors: Duyu
authors: Department of Medical Genetics, Faculty of Medicine, Ege University, Bornova, 35100 Izmir, Turkey.
authors: Burak
authors: B
authors: Durmaz
authors: Department of Medical Biology, Faculty of Medicine, Ege University, 35100 Izmir, Turkey.
authors: Cumhur
authors: C
authors: Gunduz
authors: Department of Pediatric Hematology, Behcet Uz Children's Hospital, 35210 Izmir, Turkey.
authors: Canan
authors: C
authors: Vergin
authors: Department of Pediatrics, Faculty of Medicine, Ege University, 35100 Izmir, Turkey.
authors: Deniz
authors: D
authors: Yilmaz Karapinar
authors: Department of Pediatrics, Faculty of Medicine, Ege University, 35100 Izmir, Turkey.
authors: Serap
authors: S
authors: Aksoylar
authors: Department of Pediatrics, Faculty of Medicine, Ege University, 35100 Izmir, Turkey.
authors: Kaan
authors: K
authors: Kavakli
authors: Department of Pediatrics, Faculty of Medicine, Ege University, 35100 Izmir, Turkey.
authors: Nazan
authors: N
authors: Cetingul
authors: Department of Pediatrics, Faculty of Medicine, Dokuz Eylul University, 35340 Izmir, Turkey.
authors: Gulersu
authors: G
authors: Irken
authors: Department of Pediatric Hematology, Behcet Uz Children's Hospital, 35210 Izmir, Turkey.
authors: Yontem
authors: Y
authors: Yaman
authors: Department of Pediatrics, Faculty of Medicine, Ege University, 35100 Izmir, Turkey.
authors: Ferda
authors: F
authors: Ozkinay
authors: Department of Pediatrics, Faculty of Medicine, Ege University, 35100 Izmir, Turkey.
authors: Ozgur
authors: O
authors: Cogulu
mesh_terms: D000293:Adolescent; D063646:Carcinogenesis / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology
chemical_list: D035683:MicroRNAs