title: Specific ligands for classical swine fever virus screened from landscape phage display library.
abstract: Classical swine fever (CSF) is a devastating infectious disease caused by classical swine fever virus (CSFV). The screening of CSFV-specific ligands is of great significance for diagnosis and treatment of CSF. Affinity selection from random peptide libraries is an efficient approach to discover ligands with high stability and specificity. Here, we screened phage ligands for the CSFV E2 protein from f8/8 landscape phage display library by biopanning and obtained four phage clones specific for the E2 protein of CSFV. Viral blocking assays indicated that the phage clone displaying the octapeptide sequence DRATSSNA remarkably inhibited the CSFV replication in PK-15 cells at a titer of 10(10) transduction units, as evidenced by significantly decreased viral RNA copies and viral titers. The phage-displayed E2-binding peptides have the potential to be developed as antivirals for CSF.
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China; The Laboratory for Biosensing, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors: Long
authors: L
authors: Yin
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China.
authors: Yuzi
authors: Y
authors: Luo
authors: The Laboratory for Biosensing, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China.
authors: Bo
authors: B
authors: Liang
authors: The Laboratory for Biosensing, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors: Fei
authors: F
authors: Wang
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China.
authors: Min
authors: M
authors: Du
authors: Department of Pathobiology, Auburn University, 269 Greene Hall, Auburn, AL 36849-5519, USA. Electronic address: petreva@auburn.edu.
authors: Valery A
authors: VA
authors: Petrenko
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China. Electronic address: huajiqiu@hvri.ac.cn.
authors: Hua-Ji
authors: HJ
authors: Qiu
authors: The Laboratory for Biosensing, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China. Electronic address: liuah@qibebt.ac.cn.
authors: Aihua
authors: A
authors: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism; D006691:Classical Swine Fever / Q000821:virology; D006692:Classical Swine Fever Virus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D004353:Drug Evaluation, Preclinical; D008024:Ligands; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding; D013552:Swine; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus
keywords: Antiviral activity; Classical swine fever virus; E2 protein; Landscape phage display library; Phage display; Specific ligand