title: Cytocompatibility and antibacterial properties of capping materials.
abstract: The aim of this study was to evaluate and compare the antimicrobial activity and cytocompatibility of six different pulp-capping materials: Dycal (Dentsply), Calcicur (Voco), Calcimol LC (Voco), TheraCal LC (Bisco), MTA Angelus (Angelus), and Biodentine (Septodont). To evaluate antimicrobial activity, materials were challenged in vitro with Streptococcus mutans, Streptococcus salivarius, and Streptococcus sanguis in the agar disc diffusion test. Cytocompatibility of the assayed materials towards rat MDPC-23 cells was evaluated at different times by both MTT and apoptosis assays. Results significantly differed among the different materials tested. Both bacterial growth inhibition halos and cytocompatibility performances were significantly different among materials with different composition. MTA-based products showed lower cytotoxicity and valuable antibacterial activity, different from calcium hydroxide-based materials, which exhibited not only higher antibacterial activity but also higher cytotoxicity.
authors: Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Policlinico "San Matteo", Piazzale Golgi 3, 27100 Pavia, Italy.
authors: Claudio
authors: 0000-0002-9606-6930
authors: C
authors: Poggio
authors: Research Unit on Implant Infections, Rizzoli Orthopedic Institute, DIMES of the University of Bologna Via di Barbiano 1/10, 40136 Bologna, Italy.
authors: Carla Renata
authors: CR
authors: Arciola
authors: Department of Brain and Behavioral Sciences, University of Pavia, Via Bassi 21, 27100 Pavia, Italy.
authors: Riccardo
authors: 0000-0003-4539-6670
authors: R
authors: Beltrami
authors: Department of Molecular Medicine, Center for Tissue Engineering (CIT), INSTM UdR of Pavia, University of Pavia, Viale Taramelli 3/b, 27100 Pavia, Italy ; Department of Occupational Medicine, Ergonomy and Disability, Laboratory of Nanotechnology, Salvatore Maugeri Foundation, IRCCS, Via S. Boezio 28, 27100 Pavia, Italy.
authors: Annachiara
authors: A
authors: Monaco
authors: Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Policlinico "San Matteo", Piazzale Golgi 3, 27100 Pavia, Italy.
authors: Alberto
authors: 0000-0002-6134-454X
authors: A
authors: Dagna
authors: Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Policlinico "San Matteo", Piazzale Golgi 3, 27100 Pavia, Italy.
authors: Marco
authors: M
authors: Lombardini
authors: Department of Molecular Medicine, Center for Tissue Engineering (CIT), INSTM UdR of Pavia, University of Pavia, Viale Taramelli 3/b, 27100 Pavia, Italy ; Department of Occupational Medicine, Ergonomy and Disability, Laboratory of Nanotechnology, Salvatore Maugeri Foundation, IRCCS, Via S. Boezio 28, 27100 Pavia, Italy.
authors: Livia
authors: 0000-0003-1181-3632
authors: L
authors: Visai
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001729:Bismuth / Q000009:adverse effects / Q000494:pharmacology; D017610:Calcium Compounds / Q000009:adverse effects / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000009:adverse effects / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003738:Dental Cements / Q000009:adverse effects / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008903:Minerals / Q000009:adverse effects / Q000494:pharmacology; D010087:Oxides / Q000009:adverse effects / Q000494:pharmacology; D051381:Rats; D017640:Silicates / Q000009:adverse effects / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects; D013298:Streptococcus sanguis / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; C559663:Calcicur; D017610:Calcium Compounds; D003738:Dental Cements; C475480:MTA-Angelus; D008903:Minerals; D010087:Oxides; D017640:Silicates; C506393:tricalcium silicate; C025702:Dycal; D002126:Calcium Hydroxide; D001729:Bismuth