title: The tumor suppressor role of miR-124 in osteosarcoma.
abstract: MicroRNAs have crucial roles in development and progression of human cancers, including osteosarcoma. Recent studies have shown that miR-124 was down-regulated in many cancers; however, the role of miR-124 in osteosarcoma development is unknown. In this study, we demonstrate that expression of miR-124 is significantly downregulated in osteosarcoma tissues and cell lines, compared to the adjacent tissues. The expression of miR-124 in the metastases osteosarcoma tissues was lower than that in non- metastases tissues. We identified and confirmed Rac1 as a novel, direct target of miR-124 using prediction algorithms and luciferase reporter gene assays. Overexpression of miR-124 suppressed Rac1 protein expression and attenuated cell proliferation, migration, and invasion and induced apoptosis in MG-63 and U2OS in vitro. Moreover, overexpression of Rac1 in miR-124-transfected osteosarcoma cells effectively rescued the inhibition of cell invasion caused by miR-124. Therefore, our results demonstrate that miR-124 is a tumor suppressor miRNA and suggest that this miRNA could be a potential target for the treatment of osteosarcoma in future.
authors: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang Prov., China.
authors: Shuo
authors: S
authors: Geng
authors: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang Prov., China.
authors: Xiaojun
authors: X
authors: Zhang
authors: Department of gynaecology and obstetrics, the First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang Prov., China.
authors: Jie
authors: J
authors: Chen
authors: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang Prov., China.
authors: Xing
authors: X
authors: Liu
authors: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang Prov., China.
authors: Hepeng
authors: H
authors: Zhang
authors: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang Prov., China.
authors: Xiaoyan
authors: X
authors: Xu
authors: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang Prov., China.
authors: Yan
authors: Y
authors: Ma
authors: Department of Pharmacology, Harbin Medical University, Harbin, Heilongjiang Prov., China.
authors: Baoxin
authors: B
authors: Li
authors: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang Prov., China.
authors: Yunqi
authors: Y
authors: Zhang
authors: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang Prov., China.
authors: Zhenggang
authors: Z
authors: Bi
authors: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang Prov., China.
authors: Chenglin
authors: C
authors: Yang
mesh_terms: D000293:Adolescent; D017209:Apoptosis; D045744:Cell Line, Tumor; D002648:Child; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009362:Neoplasm Metastasis; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C509207:MIRN124 microRNA, human; D035683:MicroRNAs; C107953:RAC1 protein, human; D020830:rac1 GTP-Binding Protein