title: Voltage-gated sodium channels were differentially expressed in human normal prostate, benign prostatic hyperplasia and prostate cancer cells.
abstract: Voltage-gated sodium channels (VGSCs) are expressed not only in excitable cells but also in numerous metastatic cells, particularly in certain types of cancer cells. In some types of cancer, including prostate cancer, the expression of VGSCs is associated with cancer migration, invasion and metastasisin vivo. However, the detailed expression profiles of VGSC α subunits in normal human prostate, in prostatic hyperplasia and prostatic cancer remain controversial. In the present study, quantitative polymerase chain reaction was used to systematically detect all subtypes of VGSC α subunits in normal human prostate, benign prostatic hyperplasia (BPH) and prostate cancer cells. The expression profile of VGSC α subunits was observed to differ between these cell types. Nav1.5 was the major isoform expressed in normal human prostate tissue, while Nav1.5 and Nav1.2 were the predominant isoforms in BPH tissue. However, in PC-3 and LNCaP cells, two typical prostate cancer cell lines, Nav1.6 and Nav1.7 were abundantly expressed. By comparing the relative expression levels of Nav1.5, Nav1.6 and Nav1.7 in these cells, the mRNA levels of Nav1.6 and Nav1.7 were identified to be 6- to 27-fold higher in PC-3 and LNCaP cells than in either normal or BPH samples (P<0.05); however, Nav1.5 mRNA levels were relatively lower compared with those of Nav1.6 or Nav1.7 in all cells analyzed. To confirm whether Nav1.6 and Nav1.7 expression in cancer cells was functional, a patch-clamp technique was used to record whole-cell currents. A tetrodotoxin-sensitive sodium current was successfully recorded in PC-3 cells, but not in LNCaP cells. It was concluded that although all types of VGSC α subunits exhibited low expression levels in normal prostate and BPH cells, both Nav1.6 and Nav1.7 were significantly upregulated in the prostate cancer cell lines, suggesting these subtypes may be potential diagnostic markers and therapeutic targets for certain types of prostate cancer in humans.
authors: Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors: Bin
authors: B
authors: Shan
authors: Department of Surgery, The Affiliated Hospital of Hebei Science and Technology University, Shijiazhuang, Hebei 050018, P.R. China.
authors: Mei
authors: M
authors: Dong
authors: Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors: He
authors: H
authors: Tang
authors: Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors: Na
authors: N
authors: Wang
authors: Department of Urology, The First Hospital of Shijiazhuang, Shijiazhuang, Hebei 050011, P.R. China.
authors: Jin
authors: J
authors: Zhang
authors: Department of Hepatobiliary Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors: Changqing
authors: C
authors: Yan
authors: Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors: Xiaocui
authors: X
authors: Jiao
authors: Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors: Hailin
authors: H
authors: Zhang
authors: Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors: Chuan
authors: C
authors: Wang
keywords: benign prostatic hyperplasia; cancer; mRNA; prostate; voltage-gated sodium channel