title: Mesenchymal stem cells alleviate experimental asthma by inducing polarization of alveolar macrophages.
abstract: The reparative and immunoregulatory properties of mesenchymal stromal cells (MSCs) have made them attractive candidates for cellular therapy. However, the underlying mechanism of the effects of transplanted MSCs on allergic asthma remains elusive. Here, we show that administration of MSCs isolated from human bone marrow provoked a pronounced polarization in alveolar macrophages to M2 subtypes, rather than induced an increase in the total macrophage number, and efficiently inhibited hallmark features of asthma, including airway hyperresponsiveness and eosinophilic accumulation. Moreover, transforming growth factor beta (TGF-β) signaling pathway appeared to mediate the effects of MSCs on macrophage polarization and subsequently the inhibition of hallmark features of asthma. Inhibition of TGF-β signaling was sufficient to inhibit the macrophage polarization in response to MSCs and consequently reserved the inhibitory effects of macrophage polarization on hallmark features of asthma. Collectively, our data demonstrate that human MSCs have immunosuppressive activity on asthma, which is mediated by TGF-β-signaling-dependent alveolar macrophage polarization.
authors: Department of Respiratory, Shanghai 10th People's Hospital-Tongji University School of Medicine, 301 Middle Yanchang Rd, Shanghai, 200072, China, xiaolian_song14@163.com.
authors: Xiaolian
authors: X
authors: Song
authors: Shuanshuan
authors: S
authors: Xie
authors: Kun
authors: K
authors: Lu
authors: Changhui
authors: C
authors: Wang
mesh_terms: D000818:Animals; D001249:Asthma / Q000517:prevention & control / Q000628:therapy*; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation / Q000276:immunology; D016764:Cell Polarity / Q000276:immunology*; D002478:Cells, Cultured; D004804:Eosinophils / Q000276:immunology; D006801:Humans; D007166:Immunosuppressive Agents; D016676:Macrophages, Alveolar / Q000276:immunology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010047:Ovalbumin; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014183:Transplantation, Heterologous
chemical_list: D007166:Immunosuppressive Agents; D016212:Transforming Growth Factor beta; D010047:Ovalbumin