title: Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP).
abstract: The interaction of a newly synthesized antitumor complex cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP) with DNA was investigated in vitro through a number of techniques including comet assay, immunoprecipitation, and immunolocalization of certain nucleolar proteins (the upstream binding factor (UBF) and fibrillarin) involved in DNA transcription, rRNA processing, and ribosomal assembly. The results showed that RAP binds to the DNA of two cell lines (H4 and Hs-683) causing a delay in cell proliferation rate leading to a number of cellular modifications. These modifications include DNA-damage assessed by the single cell gel electrophoresis method (comet assay) and variation in the expression of nucleolar proteins; UBF was more abundant in RAP treated cells, this was explained by the high affinity of this protein to DNA modified by RAP. On the other hand, fibrillarin was found in less quantities in RAP treated cells which was explained by a de-regulation of the ribosomal machinery caused by RAP.
authors: Fatima Azzahra
authors: FA
authors: Delmani
authors: José
authors: J
authors: Torreblanca
authors: Javier
authors: J
authors: Moreno
authors: Gregorio
authors: G
authors: García-Herdugo
authors: Rosario
authors: R
authors: Vilaplana
authors: Francisco
authors: F
authors: González-Víltchez
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004249:DNA Damage; D004353:Drug Evaluation, Preclinical; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D009942:Organometallic Compounds / Q000494:pharmacology*; D021381:Protein Transport / Q000187:drug effects; D012323:RNA Processing, Post-Transcriptional / Q000187:drug effects; D014018:Tissue Distribution / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D009687:Nuclear Proteins; D009942:Organometallic Compounds; C544015:dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetatoruthenium(III)