title: Anti-EGFR antibody conjugated organic-inorganic hybrid lipid nanovesicles selectively target tumor cells.
abstract: Chemical conjugation of anti-epidermal growth factor receptor monoclonal antibodies (anti-EGFR mAbs) to organic-inorganic hybrid liposomal immunocerasomes via maleimide-thiol coupling chemistry is explored as a mechanism for selectively targeting cancer cells. The cellular uptake and internalization of immunocerasomes are investigated in A431 cells that express an abnormally high level of EGFR, DU145 cells that overexpress EGFR, and HL-60 cells that are used as a negative control. The internalization study reveals a strong correlation between the receptor-mediated endocytosis of immunocerasomes and the membrane expression of EGFR. Further, free anti-EGFR mAbs and immunocerasomes conjugated with anti-EGFR mAbs at nanomolar doses display similar anti-proliferative effects on A431 cells. Additionally, serum proteins greatly reduce the cellular uptake of cerasomes that is mediated by non-specific receptors, but have no adverse effects on the specific EGFR-mediated delivery of immunocerasomes to A431 cells.
authors: Department of Aerospace and Mechanical Engineering, the University of Arizona, Tucson, AZ 85721, USA.
authors: Siu Ling
authors: SL
authors: Leung
authors: Department of Aerospace and Mechanical Engineering, the University of Arizona, Tucson, AZ 85721, USA; Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, People's Republic of China.
authors: Zhengbao
authors: Z
authors: Zha
authors: Biomedical Engineering and Bio5 Institute, The University of Arizona, Tucson, AZ 85721, USA.
authors: Celine
authors: C
authors: Cohn
authors: Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, People's Republic of China.
authors: Zhifei
authors: Z
authors: Dai
authors: Department of Aerospace and Mechanical Engineering, the University of Arizona, Tucson, AZ 85721, USA; Biomedical Engineering and Bio5 Institute, The University of Arizona, Tucson, AZ 85721, USA. Electronic address: xwu@arizona.edu.
authors: Xiaoyi
authors: X
authors: Wu
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004705:Endocytosis / Q000187:drug effects; D066246:ErbB Receptors / Q000276:immunology*; D006801:Humans; D007287:Inorganic Chemicals / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D007287:Inorganic Chemicals; D008055:Lipids; D009930:Organic Chemicals; D053835:Unilamellar Liposomes; D066246:ErbB Receptors
keywords: Anti-EGFR antibody; Cancer cells; Organic–inorganic hybrid lipid nanovesicles; Selective targeting