title: Optical-resolution photoacoustic microscopy for volumetric and spectral analysis of histological and immunochemical samples.
abstract: Optical-resolution photoacoustic microscopy (OR-PAM) is an imaging modality with superb penetration depth and excellent absorption contrast. Here we demonstrate, for the first time, that this technique can advance quantitative analysis of conventional chromogenic histochemistry. Because OR-PAM can quantify the absorption contrast at different wavelengths, it is feasible to spectrally resolve the specific biomolecules involved in a staining color. Furthermore, the tomographic capability of OR-PAM allows for noninvasive volumetric imaging of a thick sample without microtoming it. By immunostaining the sample with different chromogenic agents, we further demonstrated the ability of OR-PAM to resolve different types of cells in a coculture sample with imaging depths up to 1 mm. Taken together, the integration of OR-PAM with (immuno)histochemistry offers a simple and versatile technique with broad applications in cell biology, pathology, tissue engineering, and related biomedical studies.
authors: The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332 (USA).
authors: Yu Shrike
authors: YS
authors: Zhang
authors: Junjie
authors: J
authors: Yao
authors: Chi
authors: C
authors: Zhang
authors: Lei
authors: L
authors: Li
authors: Lihong V
authors: LV
authors: Wang
authors: Younan
authors: Y
authors: Xia
mesh_terms: D008853:Microscopy / Q000379:methods*; D061088:Photoacoustic Techniques; D013056:Spectrophotometry, Ultraviolet
keywords: biomedical imaging; cell biology; histochemistry; photoacoustic microscopy; tissue engineering