title: [Preparation and characterization of genipin-crosslinked silk fibroin/chitosan controlled-release microspheres].
abstract: OBJECTIVE
  To investigate the property of genipin-crosslinked silk fibroin(SF)/chitosan(CS) microspheres for slow releasing of bovine serum albumin (BSA).
  
  
  METHODS
  BSA-loaded genipin-crosslinked SF/CS microspheres were prepared by emulsion cross-linking technique. The micropheres were observed for surface morphology and size distribution under scanning electron microscope (SEM), and X-ray diffractometry (XRD) and fourier transform infrared spectroscopy (FTIR) were used to analyze their structural characteristics. BCA method was used for determining the drug entrapment, loading rate and cumulative drug release in 21 days.
  
  
  RESULTS
  The microspheres were spherical and showed a smooth surface with an average diameter of 7.84∓0.97 µm. The drug entrapment efficiency of the microspheres was (50.16∓4.32)% with a drug loading ratio of (1.25∓0.11)% and a cumulative release of the total drug of (75.2∓2.53)% in 21 days.
  
  
  CONCLUSIONS
  Genipin-crosslinked SF/CS microspheres have a high drug entrapment efficiency and possess good capacity of sustained drug release.
authors: Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou 510220, China.E-mail: spiritsealove@126.com.
authors: Manwen
authors: M
authors: Ye
authors: Shuguang
authors: S
authors: Zeng
authors: Wenfeng
authors: W
authors: Gao
authors: Mingdeng
authors: M
authors: Rong
authors: Zehong
authors: Z
authors: Guo
authors: Yong
authors: Y
authors: Shi
authors: Huiyong
authors: H
authors: Gan
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004655:Emulsions; D005349:Fibroins / Q000737:chemistry*; D039823:Iridoids / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction; D014965:X-Rays
chemical_list: D003692:Delayed-Action Preparations; D004655:Emulsions; D039823:Iridoids; D005349:Fibroins; D048271:Chitosan; C007834:genipin