title: Tanshinones extend chronological lifespan in budding yeast Saccharomyces cerevisiae.
abstract: Natural products with anti-aging property have drawn great attention recently but examples of such compounds are exceedingly scarce. By applying a high-throughput assay based on yeast chronological lifespan measurement, we screened the anti-aging activity of 144 botanical materials and found that dried roots of Salvia miltiorrhiza Bunge have significant anti-aging activity. Tanshinones isolated from the plant including cryptotanshione, tanshinone I, and tanshinone IIa, are the active components. Among them, cryptotanshinone can greatly extend the budding yeast Saccharomyces cerevisiae chronological lifespan (up to 2.5 times) in a dose- and the-time-of-addition-dependent manner at nanomolar concentrations without disruption of cell growth. We demonstrate that cryptotanshinone prolong chronological lifespan via a nutrient-dependent regime, especially essential amino acid sensing, and three conserved protein kinases Tor1, Sch9, and Gcn2 are required for cryptotanshinone-induced lifespan extension. In addition, cryptotanshinone significantly increases the lifespan of SOD2-deleted mutants. Altogether, those data suggest that cryptotanshinone might be involved in the regulation of, Tor1, Sch9, Gcn2, and Sod2, these highly conserved longevity proteins modulated by nutrients from yeast to humans.
authors: Food Science and Technology Programme, Department of Chemistry, National University of Singapore, Science Drive 3, Singapore, 117543, Republic of Singapore.
authors: Ziyun
authors: Z
authors: Wu
authors: Lixia
authors: L
authors: Song
authors: Shao Quan
authors: SQ
authors: Liu
authors: Dejian
authors: D
authors: Huang
mesh_terms: D045784:Abietanes / Q000302:isolation & purification / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D017353:Gene Deletion; D006801:Humans; D050296:Microbial Viability / Q000187:drug effects*; D010616:Phenanthrenes / Q000302:isolation & purification / Q000378:metabolism*; D064209:Phytochemicals / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots; D011494:Protein Kinases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000502:physiology*; D027544:Salvia / Q000737:chemistry; D013482:Superoxide Dismutase / Q000172:deficiency; D013997:Time Factors; D000099156:Superoxide Dismutase 2
chemical_list: D045784:Abietanes; D000596:Amino Acids; D010616:Phenanthrenes; D064209:Phytochemicals; D011494:Protein Kinases; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2; C021751:tanshinone; C037886:cryptotanshinone