title: Characterisation of kisspeptin system genes in an ovoviviparous teleost: Sebastes schlegeli.
abstract: Kisspeptins are neuropeptides that play important roles in the reproduction and the onset of puberty in vertebrate by activating their receptor, Kissr. In the present study, we first isolated kiss1 and kissr4 genes from an ovoviviparous fish, the black rockfish (Sebastes schlegeli) by homologue cloning. Phylogenetic analysis indicated that the kiss and kissr of S. schlegeli belonged to kiss1 and kissr4 respectively. Quantitative real-time PCR analysis showed that the kissr4 was expressed mainly in the brain and testis, while the kiss1 was expressed predominantly in the heart of both sexes. As for the different gonadal maturation stages the kiss1 showed different expression patterns in different tissues. During the early development stage, expression levels of the ligand and receptor genes showed similar increasing trends. The promoter region of kissr4 contained several putative transcription factor (TF) binding sites which may have the function of regulating kisspeptin system gene expression, providing potential targets for future in-depth investigation. These results together confirmed that the kisspeptin system in S. schlegeli may be involved in reproduction and other activities. Furthermore, our study laid the groundwork for further learning about the evolution and function of kisspeptin system in fish even vertebrate.
authors: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors: Huayu
authors: H
authors: Song
authors: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors: Yan
authors: Y
authors: He
authors: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors: Liman
authors: L
authors: Ma
authors: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors: Xiaosu
authors: X
authors: Zhou
authors: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors: Xiumei
authors: X
authors: Liu
authors: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China. Electronic address: qijie@ouc.edu.cn.
authors: Jie
authors: J
authors: Qi
authors: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China. Electronic address: qzhang@ouc.edu.cn.
authors: Quanqi
authors: Q
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D005260:Female; D005399:Fishes / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006066:Gonads / Q000378:metabolism*; D006801:Humans; D059648:Kisspeptins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D052285:Ovoviviparity / Q000502:physiology*; D010802:Phylogeny; D012098:Reproduction / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D012725:Sexual Behavior; D012741:Sexual Maturation
chemical_list: D059648:Kisspeptins
keywords: Expression pattern; Kisspeptin receptor; Promoter analysis; Sebastes schlegeli; kiss1