title: Targeting synaptic pathology with a novel affinity mass spectrometry approach.
abstract: We report a novel strategy for studying synaptic pathology by concurrently measuring levels of four SNARE complex proteins from individual brain tissue samples. This method combines affinity purification and mass spectrometry and can be applied directly for studies of SNARE complex proteins in multiple species or modified to target other key elements in neuronal function. We use the technique to demonstrate altered levels of presynaptic proteins in Alzheimer disease patients and prion-infected mice.
authors: From the ‡Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, S43180 Mölndal, Sweden; ann.brinkmalm@neuro.gu.se.
authors: Ann
authors: A
authors: Brinkmalm
authors: From the ‡Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, S43180 Mölndal, Sweden;
authors: Gunnar
authors: G
authors: Brinkmalm
authors: ¶Department of Psychiatry, University of British Columbia, Vancouver V6H3Z6, British Columbia, Canada;
authors: William G
authors: WG
authors: Honer
authors: ‖MRC Toxicology Unit, Hodgkin Building, University of Leicester, LE19HN Leicester, UK;
authors: Julie A
authors: JA
authors: Moreno
authors: From the ‡Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, S43180 Mölndal, Sweden;
authors: Joel
authors: J
authors: Jakobsson
authors: ‖MRC Toxicology Unit, Hodgkin Building, University of Leicester, LE19HN Leicester, UK;
authors: Giovanna R
authors: GR
authors: Mallucci
authors: From the ‡Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, S43180 Mölndal, Sweden; **UCL Institute of Neurology, Queen Square WC1N3BG, London, UK.
authors: Henrik
authors: H
authors: Zetterberg
authors: From the ‡Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, S43180 Mölndal, Sweden;
authors: Kaj
authors: K
authors: Blennow
authors: From the ‡Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, S43180 Mölndal, Sweden;
authors: Annika
authors: A
authors: Öhrfelt
mesh_terms: D000375:Aging / Q000378:metabolism / Q000502:physiology*; D000544:Alzheimer Disease / Q000378:metabolism / Q000503:physiopathology*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D004195:Disease Models, Animal; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D017096:Prion Diseases / Q000378:metabolism / Q000503:physiopathology / Q000662:veterinary; D040901:Proteomics / Q000379:methods; D050600:SNARE Proteins / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism* / Q000473:pathology
chemical_list: D050600:SNARE Proteins