title: Carbon nanotubes functionalized electrospun nanofibers formed 3D electrode enables highly strong ECL of peroxydisulfate and its application in immunoassay.
abstract: A new biosensing platform based on electrospun carbon nanotubes nanofibers (CNTs@PNFs) composite, which enabled strong electrochemiluminescent emission of peroxydisulfate, was firstly developed for immunoassay with favorable analytical performances, and then was utilized to evaluate the interaction between antibody and antigen in vitro. Moreover, the obvious ECL image of peroxydisulfate on the prepared sensing platform was firstly recorded in this report. In order to expand the application of peroxydisulfate ECL, the specific recognization biomolecules, α-fetoprotein (AFP) antibody was bound to the functionalized film via electrostatic interaction for fabricating label-free ECL immunosensor to detect α-AFP. Based on the ECL change resulting from the specific immunoreaction between antigen and antibody, the quantitative analysis for AFP with wide dynamic response in the range from 0.1 pg mL(-1) to 160 ng mL(-1) was realized. And the limit of detection was estimated to be 0.09 pg mL(-1). Therefore, the flexible sensing platform not only acted as the sensitized sensing element, but also offered a suitable carrier for immobilization of biological recognition elements with low-toxicity and eco-friendliness, which opened a promising approach to developing further electrospun nanofiber based amplified ECL biosensor with favorable analytical performances.
authors: College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350108, Fujian, China. Electronic address: dhong@fjnu.edu.cn.
authors: Hong
authors: H
authors: Dai
authors: College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350108, Fujian, China.
authors: Guifang
authors: G
authors: Xu
authors: College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350108, Fujian, China.
authors: Shupei
authors: S
authors: Zhang
authors: College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350108, Fujian, China.
authors: Lingshan
authors: L
authors: Gong
authors: College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350108, Fujian, China.
authors: Xiuhua
authors: X
authors: Li
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou 350002, Fujian, China.
authors: Caiping
authors: C
authors: Yang
authors: College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350108, Fujian, China; Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou 350002, Fujian, China.
authors: Yanyu
authors: Y
authors: Lin
authors: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, Fujian, China. Electronic address: cjh_huaxue@126.com.
authors: Jinghua
authors: J
authors: Chen
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou 350002, Fujian, China.
authors: Guonan
authors: G
authors: Chen
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D004566:Electrodes; D004867:Equipment Design; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D013431:Sulfates / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D037742:Nanotubes, Carbon; D013431:Sulfates; D000509:alpha-Fetoproteins
keywords: Electrospun nanofibers; Immunoassay; Peroxydisulfate