title: Oxytocin improves follicular reserve in a cisplatin-induced gonadotoxicity model in rats.
abstract: Cisplatin (CP), an antitumor agent, has been shown to cause ovarian injury and dysfunction in both animal and human studies. The present study was conducted to investigate the protective effect of oxytocin (OT) on CP-induced ovarian toxicity in rats. Twenty-one adult female rats were included in the study. Fourteen rats were administered intraperitoneally CP (2 mg/kg/day) twice a week for 5 weeks. Control group (n = 7) did not receive any treatment. Following treatment, CP-received rats were randomly divided into two groups and treated with either saline (1 mL/kg/day, n = 7) or OT (160 μg/kg/day, n = 7) for 5 weeks. Then, ovarian toxicity and effects of OT were evaluated by histomorphological and biochemical analysis. Our findings revealed a significant reduction in the number of follicles at each grade in saline-treated group. AMH level was significantly lower in saline group compared to control (P < 0.0005). OT treatment significantly attenuated CP toxicity in ovaries and increased AMH levels compared to saline group (P < 0.005). Also, administration of OT lessened lipid peroxidation and prevented glutathione depletion in CP-treated rats (P < 0.05). These results indicated that OT could lessen the CP-induced ovarian damage and improve follicular reserve by preventing oxidative damage.
authors: Department of Physiology, Ege University School of Medicine, 35100 Izmir, Turkey.
authors: Oytun
authors: O
authors: Erbaş
authors: Department of Obstetrics and Gynecology, Ege University School of Medicine, 35100 Izmir, Turkey.
authors: Levent
authors: L
authors: Akman
authors: Department of Histology and Embryology, Ege University School of Medicine, 35100 Izmir, Turkey.
authors: Altuğ
authors: A
authors: Yavaşoğlu
authors: Department of Obstetrics and Gynecology, Ege University School of Medicine, 35100 Izmir, Turkey.
authors: Mustafa Cosan
authors: MC
authors: Terek
authors: Division of Medical Oncology, Tepecik Educational and Training Hospital, 35100 Izmir, Turkey.
authors: Tülay
authors: T
authors: Akman
authors: Department of Physiology, Ege University School of Medicine, 35100 Izmir, Turkey.
authors: Dilek
authors: 0000-0002-4505-0939
authors: D
authors: Taskiran
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002945:Cisplatin / Q000009:adverse effects*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006066:Gonads / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D006080:Ovarian Follicle / Q000187:drug effects* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D010121:Oxytocin / Q000008:administration & dosage*; D051381:Rats
chemical_list: D000975:Antioxidants; D010121:Oxytocin; D005978:Glutathione; D002945:Cisplatin