title: [The spread of KPC-3 Klebsiella pneumoniae in hospitals in Bogotá over a three-year period (2008-2010)].
abstract: Introduction : One of the major worldwide public health problems today are the infections caused by carbapenem-resistant Enterobacteriaceae (CRE), among which carbapenem-resistant Klebsiella pneumoniae (CRKP), constitutes one of the most common pathogens causing nosocomial infection.
  
  
  OBJECTIVE
  This study was aimed at describing the dissemination of KPC-3 enzyme-producing Klebsiella pneumoniae in clinical isolates from hospitals in Bogotá.
  
  
  METHODS
  Eighty-two CRKP isolates collected from 10 hospitals in Bogotá from 2008-2010 were analysed; disk diffusion and microdilution were used for phenotypic detection of enzymes and PCR for genotyping. Automated and manual methods were used for determining profiles for antimicrobial susceptibility testing (AST) with 13 agents. PFGE was used for obtaining the isolates´ genetic relationship.
  
  
  RESULTS
  This study gives an overview of CRKP patterns in 10 hospitals in Bogota which were found to present resistance to multiple antibiotic families. The CRKPs were grouped in different clones, each having different subtypes, and were spread in the 10 hospitals over the three-year period (2008-2010).
  
  
  CONCLUSIONS
  The dissemination of KPC-3-producing Klebsiella pneumoniae nosocomial isolates in Bogota highlights the need for strengthening epidemiological surveillance against this type of microorganism and the development of specific priority activities for preventing and controlling such infection.
authors: Grupo de Resistencia Bacteriana de Bogotá (GREBO), Bogotá, D.C, Colombia.
authors: Edna Catering
authors: EC
authors: Rodríguez
authors: Grupo de Resistencia Bacteriana de Bogotá (GREBO), Bogotá, D.C, Colombia.
authors: Sandra Yamile
authors: SY
authors: Saavedra
authors: Grupo de Resistencia Bacteriana de Bogotá (GREBO), Bogotá, D.C, Colombia.
authors: Aura Lucía
authors: AL
authors: Leal
authors: Hospital Universitario San Ignacio, Bogotá, D.C, Colombia.
authors: Carlos
authors: C
authors: Álvarez
authors: Hospital El Tunal, Bogotá, D.C, Colombia.
authors: Narda
authors: N
authors: Olarte
authors: Hospital El Tunal, Bogotá, D.C, Colombia.
authors: Alberto
authors: A
authors: Valderrama
authors: Instituto Nacional de Cancerología, Bogotá, D.C, Colombia.
authors: Sonia Isabel
authors: SI
authors: Cuervo
authors: Instituto de Genética Molecular, Universidad El Bosque, Bogotá, D.C, Colombia.
authors: Javier
authors: J
authors: Escobar
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques; D002999:Clone Cells; D003105:Colombia / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006786:Hospitals, Urban / Q000706:statistics & numerical data; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests / Q000379:methods; D011159:Population Surveillance; D062606:Tertiary Care Centers / Q000706:statistics & numerical data; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C495220:beta-lactamase KPC-3, Klebsiella pneumoniae