title: Protective effects of polyphenols-enriched extract from Huangshan Maofeng green tea against CCl4-induced liver injury in mice.
abstract: The study was to characterize the polyphenolic composition, antioxidant properties, and hepatoprotective effects of a polyphenols-enriched extract (HMTP) from Huangshan Maofeng green tea. HPLC analysis showed that three predominantly polyphenolic compounds present in HMTP were epigallocatechin (271.2 μg/mg extract), rutin (239.3 μg/mg) and epicatechin (89.3 μg/mg). HMTP was shown to exhibit strong scavenging activities against DPPH, O2(-), and OH, and ferric-reducing antioxidant power in vitro. Administration of HMTP at 200, 400 and 800 mg/kg bw in mice prior to CCl4 injury significantly decreased the CCl4-induced elevation of serum ALT, AST and ALP activities, and prevented an increase in hepatic MDA levels (p<0.05). Mice with HMTP pretreatment displayed a better profile of hepatosomatic index and the improved GSH-Px and SOD activities in the liver, relative to CCl4-intoxicated mice. Liver pathological observation also confirmed the protection on CCl4-caused histological alteration, suggesting that HMTP has potential to be explored as valuable hepatoprotective function food.
authors: School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
authors: Yanmang
authors: Y
authors: Cui
authors: College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
authors: Xingbin
authors: X
authors: Yang
authors: College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
authors: Xinshan
authors: X
authors: Lu
authors: School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
authors: Jinwen
authors: J
authors: Chen
authors: School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China. Electronic address: yanzhao@fmmu.edu.cn.
authors: Yan
authors: Y
authors: Zhao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride / Q000633:toxicity; D002392:Catechin / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D002851:Chromatography, High Pressure Liquid; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D012431:Rutin / Q000737:chemistry / Q000494:pharmacology; D013662:Tea / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D013662:Tea; D012431:Rutin; D002392:Catechin; D002251:Carbon Tetrachloride; C057580:gallocatechol
keywords: Antioxidant activity; HPLC; Hepatoprotective effects; Huangshan Maofeng green tea; Polyphenols