title: Allogeneic DCG promote lung NK cell activation and antitumor effect after invariant NKT cell activation.
abstract: OBJECTIVE
  We aimed to investigate whether allogeneic dendritic cells pulsed with alpha-galactosylceramide (DCG) treatment induces activation of Natural Killer T (NKT) cells.
  
  
  METHODS
  C57BL/6 and BALB/c mice were injected with syngeneic and allogeneic DCG. We then examined NK and NKT cell activity in the lung and spleen and antitumor effect in various lung tumor metastatic models.
  
  
  RESULTS
  While splenic NKT activity was suppressed after allogeneic DCG treatment, allogeneic DCG treatment induced similar antitumor effect as well as lung NKT and NK cell activity compared to syngeneic DCG treatment. Furthermore, allogeneic DCG treatment prolonged survival in B16F10, LLC, Pan02, MethA, and CT26 tumor models in two strains of mice.
  
  
  CONCLUSIONS
  This allogeneic DCG treatment could be substituted for syngeneic DCG treatment. The results obtained in the present study suggest that allogeneic DCG treatment may provide a new approach in cancer immunotherapy with NKT cells.
authors: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: Hiroshi
authors: H
authors: Hasegawa
authors: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan kiyama@med.kobe-u.ac.jp.
authors: Kimihiro
authors: K
authors: Yamashita
authors: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: Dai
authors: D
authors: Otubo
authors: Laboratory for Immunotherapy, RIKEN Center for integrative Medical Science (IMS-RCAI), Yokohama, Japan.
authors: Shin-Ichiro
authors: S
authors: Fujii
authors: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: Takashi
authors: T
authors: Kamigaki
authors: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: Daisuke
authors: D
authors: Kuroda
authors: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: Yoshihiro
authors: Y
authors: Kakeji
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000276:immunology; D005260:Female; D005699:Galactosylceramides / Q000276:immunology / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D008175:Lung Neoplasms / Q000188:drug therapy / Q000276:immunology / Q000556:secondary; D008213:Lymphocyte Activation / Q000187:drug effects; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy* / Q000276:immunology; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000187:drug effects* / Q000276:immunology*
chemical_list: D005699:Galactosylceramides; C493154:alpha-galactosylceramide; D007371:Interferon-gamma
keywords: NK cells; NKT cells; allogeneic cells; dendritic cells; tumor immunity