title: The expression of S100P increases and promotes cellular proliferation by increasing nuclear translocation of β-catenin in endometrial cancer.
abstract: There is increasing evidence suggesting that S100P has a significant role in cancer, and is associated with poor clinical outcomes. The expression of S100P mRNA and protein in endometrial cancer and normal endometrium tissues was detected by real-time quantitative RT-PCR and immunohistochemistry. Moreover, we reduced the expression of S100P in HEC-1A and Ishikawa endometrial cancer cell lines by siRNA transfection. Based on the reduced S100P mRNA expression, we measured the effects of S100P on cellular proliferation by the cell-counting kit-8. Nuclear β-catenin protein level was detected by western blotting. Cyclin D1 and c-myc mRNA expression regulated by β-catenin was detected by real-time quantitative RT-PCR. We found that the expression of S100P mRNA and protein increased in endometrial cancer tissues compared with the normal endometrium. Local S100P expression progressively increased from pathologic differenciation grade 1 to 3. After reducing the S100P expression, the cellular proliferation ability, nuclear β-catenin protein level, cyclin D1 and c-myc mRNA levels reduced. It indicated that S100P could promote cell proliferation by increasing nuclear translocation of β-catenin. The expression of S100P mRNA and protein in endometrial cancer significantly increased and is associated with pathologic differenciation grade. S100P may promote endometrial cell proliferation by increasing nuclear translocation of β-catenin.
authors: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong Province, China.
authors: Luyan
authors: L
authors: Guo
authors: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong Province, China.
authors: Shuqin
authors: S
authors: Chen
authors: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong Province, China.
authors: Hongye
authors: H
authors: Jiang
authors: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong Province, China.
authors: Jiaming
authors: J
authors: Huang
authors: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong Province, China.
authors: Wenyan
authors: W
authors: Jin
authors: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong Province, China.
authors: Shuzhong
authors: S
authors: Yao
mesh_terms: D021581:Active Transport, Cell Nucleus; D000328:Adult; D000368:Aged; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009367:Neoplasm Staging; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D015854:Up-Regulation; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: C530104:CCND1 protein, human; C495270:CTNNB1 protein, human; D002135:Calcium-Binding Proteins; C489427:MYC protein, human; D009363:Neoplasm Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C103003:S100P protein, human; D051176:beta Catenin; D019938:Cyclin D1
keywords: S100P; c-myc; cyclinD1; endometrial cancer; proliferation; β-catenin