title: Gene expression in snapbeans exposed to ozone and protected by ethylenediurea.
abstract: Ethylenediurea (EDU) is the most common chemical used to prevent ozone (O3) injury on vegetation. Despite considerable research, its mode of action remains elusive and gene expression has not been studied. Transcripts of major antioxidant enzymes (catalase, glutathione reductase, glutathione peroxidase) were measured for the first time in a model plant (Phaseolus vulgaris cv S156) after short-term O3 exposure (0 or 90 ppb, 5 h/d, 4 days) and a single spray with EDU (0 or 300 ppm). Visible, physiological and biochemical parameters were assessed as indices of O3-induced stress. In O3-exposed EDU-protected plants, levels of transcript, enzyme activity, H2O2 accumulation, gas exchange and foliar visible injury were similar to those in control plants. These results suggest that EDU may halt the O3-induced ROS generation within 24 h from the exposure, and thus the downstream cascade mechanisms leading to increased H2O2 production, impaired gas exchange, and occurrence of leaf lesions.
authors: Institute of Sustainable Plant Protection, National Council of Research, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy. Electronic address: elena.paoletti@cnr.it.
authors: E
authors: E
authors: Paoletti
authors: Department of Agricultural, Ambient and Agro-Food Sciences, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
authors: A
authors: A
authors: Castagna
authors: Department of Chemistry, Biology and Biotechnology, University of Perugia, Borgo XX Giugno 74, I-06121 Perugia, Italy.
authors: L
authors: L
authors: Ederli
authors: Department of Chemistry, Biology and Biotechnology, University of Perugia, Borgo XX Giugno 74, I-06121 Perugia, Italy.
authors: S
authors: S
authors: Pasqualini
authors: Department of Agricultural, Ambient and Agro-Food Sciences, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
authors: A
authors: A
authors: Ranieri
authors: Department of Plant, Soil and Insect Sciences, University of Massachusetts, Amherst, MA, USA.
authors: W J
authors: WJ
authors: Manning
mesh_terms: D002374:Catalase / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005979:Glutathione Peroxidase / Q000235:genetics; D005980:Glutathione Reductase / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism; D010126:Ozone / Q000378:metabolism*; D027805:Phaseolus / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D010671:Phenylurea Compounds / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics; D020011:Protective Agents / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D010671:Phenylurea Compounds; D010940:Plant Proteins; D020011:Protective Agents; C032766:ethylene diurea; D010126:Ozone; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase
keywords: Bean; EDU; Gene expression; Gene transcription; Ground-level ozone; Phaseolus vulgaris