title: Mapping the calcification of bovine pericardium in rat model by enhanced micro-computed tomography.
abstract: The calcification initiation and progression of bioprosthetic heart valve were investigated in a rat model by enhanced micro-computed tomography, together with histologic study and scanning electron microscope analysis. The implantation data at early stage showed apparent dendritic patterns in the radiographic images for the glutaraldehyde-treated bovine pericardium and this dendritic pattern was verified to be associated with the vessel distribution in the tissue. Histologic study and scanning electron microscope analysis both indicated that the calcium deposits in the pericardium vessels regions were more grievous than those scattered in the collagen fibers in the first two weeks after implantation. Subsequently, calcification spreaded and the entire sample was severely calcified in 60 days.
authors: College of Materials Science and Engineering, Chongqing University, Chongqing 400044, China.
authors: Jing
authors: J
authors: Liu
authors: College of Materials Science and Engineering, Chongqing University, Chongqing 400044, China.
authors: Shengping
authors: S
authors: Zhong
authors: Testing Department of Biomaterials and Tissue Engineering Products, Chinese National Institutes for Food and Drug Control, Beijing 100050, China.
authors: Hualin
authors: H
authors: Lan
authors: Beijing Heart Lung Blood-Vessel Medical Center, Beijing Anzhen Hospital, Beijing University of Medical Science, Beijing 100029, China.
authors: Xu
authors: X
authors: Meng
authors: Beijing Heart Lung Blood-Vessel Medical Center, Beijing Anzhen Hospital, Beijing University of Medical Science, Beijing 100029, China.
authors: Haibo
authors: H
authors: Zhang
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, China.
authors: Yubo
authors: Y
authors: Fan
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, China.
authors: Yuxing
authors: Y
authors: Wang
authors: Testing Department of Biomaterials and Tissue Engineering Products, Chinese National Institutes for Food and Drug Control, Beijing 100050, China.
authors: Chunren
authors: C
authors: Wang
authors: Testing Department of Biomaterials and Tissue Engineering Products, Chinese National Institutes for Food and Drug Control, Beijing 100050, China. Electronic address: wangzx@nicpbp.org.cn.
authors: Zhaoxu
authors: Z
authors: Wang
mesh_terms: D000818:Animals; D001705:Bioprosthesis / Q000009:adverse effects*; D002114:Calcinosis / Q000000981:diagnostic imaging / Q000209:etiology* / Q000473:pathology; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002417:Cattle; D006350:Heart Valve Prosthesis / Q000009:adverse effects*; D008297:Male; D010496:Pericardium / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D017208:Rats, Wistar; D055114:X-Ray Microtomography
chemical_list: D002118:Calcium
keywords: Bioprosthetic heart valve; Bovine pericardium; Calcification; Micro-computed tomography (micro-CT)