title: Bacterial overexpression of recombinant heteroscorpine-1 (rHS-1), a toxin from Heterometrus laoticus scorpion venom: trends for antibacterial application and antivenom production.
abstract: Heteroscorpine-1 (HS-1) was identified as a member of the scorpine family. HS-1 shows insecticidal activities, exhibiting a low median lethal dose (LD50) in mealworm (Tenebrio molitor L.) and inhibitory activities against Bacillus subtilis, Klebsiella pneumoniae, and Pseudomonas aeruginosa. In this study, a recombinant HS-1 (rHS-1) was produced by overexpression in E. coli. A large yield of product was obtained. The structure of purified rHS-1 was confirmed through mass spectrometry. Both anti-crude venom and anti-rHS-1 antibodies specifically recognized rHS-1, suggesting its structural similarity. Reactivated rHS-1 caused roughening and blebbing of bacterial cell surfaces. It showed higher activity than that of pre-refolded protein. Antisera raised against a partially purified and mis- or unfolded peptide can inhibit relevant bioactivity.
authors: Department of Biochemistry, Faculty of Science, Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Khon Kaen University, Khon Kaen, 40002, Thailand.
authors: Nunthawun
authors: N
authors: Uawonggul
authors: Sophida
authors: S
authors: Sukprasert
authors: Paroonkorn
authors: P
authors: Incamnoi
authors: Rina
authors: R
authors: Patramanon
authors: Sompong
authors: S
authors: Thammasirirak
authors: Sutthidech
authors: S
authors: Preecharram
authors: Wandee
authors: W
authors: Bunyatratchata
authors: Buabarn
authors: B
authors: Kuaprasert
authors: Jureerut
authors: J
authors: Daduang
authors: Sakda
authors: S
authors: Daduang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D057134:Antibodies, Neutralizing / Q000276:immunology; D000997:Antivenins; D003429:Cross Reactions; D007306:Insecticides; D058849:Protein Refolding; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D012604:Scorpion Venoms / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D013712:Tenebrio
chemical_list: D000900:Anti-Bacterial Agents; D057134:Antibodies, Neutralizing; D000997:Antivenins; D007306:Insecticides; D011994:Recombinant Proteins; D012604:Scorpion Venoms; C517468:heteroscorpine-1, Heterometrus laoticus