title: Cloning and characterization of low-molecular-weight glutenin subunit alleles from Chinese wheat landraces (Triticum aestivum L.).
abstract: Low-molecular-weight glutenin subunits (LMW-GS) are of great importance in processing quality and participate in the formation of polymers in wheat. In this study, eight new LMW-GS alleles were isolated from Chinese wheat landraces (Triticum aestivum L.) and designated as Glu-A3-1a, Glu-A3-1b, Glu-B3-1a, Glu-B3-1b, Glu-B3-1c, Glu-D3-1a, Glu-D3-1b, and Glu-D3-1c, which were located at the Glu-A3, Glu-B3, and Glu-D3 loci, respectively. Based on the proteins encoded, the number of deduced amino acids of Glu-B3 alleles was approximately 50 more than those of Glu-A3 and Glu-D3 alleles. The first cysteine of Glu-A3 and Glu-D3 alleles was located at the N-terminal domain, while that of Glu-B3 alleles was found in the repetitive domain, which may lead to the different functioning in forming disulfide bonds. All the eight genes were LMW-m types and the new allele of Glu-B3-1a which had nine cysteine residues may be the desirable LMW-GS gene for improving bread-making quality.
authors: School of Agronomy, Anhui Agricultural University, Hefei 230036, China ; Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036, China.
authors: Hongqi
authors: 0000-0002-7431-3566
authors: H
authors: Si
authors: School of Agronomy, Anhui Agricultural University, Hefei 230036, China.
authors: Manli
authors: M
authors: Zhao
authors: School of Agronomy, Anhui Agricultural University, Hefei 230036, China.
authors: Xin
authors: X
authors: Zhang
authors: School of Agronomy, Anhui Agricultural University, Hefei 230036, China.
authors: Guoliang
authors: G
authors: Yao
authors: School of Agronomy, Anhui Agricultural University, Hefei 230036, China ; Biology Department, Saint Mary's University, Halifax, NS, Canada B3H 3C3.
authors: Genlou
authors: G
authors: Sun
authors: School of Agronomy, Anhui Agricultural University, Hefei 230036, China ; Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036, China.
authors: Chuanxi
authors: 0000-0003-1042-036X
authors: C
authors: Ma
mesh_terms: D000483:Alleles; D005983:Glutens / Q000737:chemistry / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics* / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D005983:Glutens; C037302:glutenin