title: Improved high sensitivity analysis of polyphenols and their metabolites by nano-liquid chromatography-mass spectrometry.
abstract: This study was conducted to assess the value of a high resolution, high mass accuracy time-of-flight analyzer in combination with nanoliquid chromatography for the analysis of polyphenols and their metabolites. The goal was to create a method that utilizes small volumes of biological fluids and provides a significant improvement in sensitivity compared with existing methods. Accordingly, nanoLC-MS and nanoLC-pseudo-multiple reaction monitoring (MRM) methods were developed that had a lower limit of quantification of 0.5 nM for several polyphenols and were linear over 2-3 orders of magnitude (R(2)>0.999). Using urine samples, the ability to observe and quantify polyphenols in such a complex biological fluid depended on much narrower mass windows (0.050 amu or less) on a TOF analyzer than those used on a quadrupole analyzer (0.7 amu). Although a greater selectivity was possible with the low mass resolution of a triple quadrupole instrument using the MRM approach, for the daidzein metabolite O-DMA, a chromatographically resolvable second peak could only be substantially reduced by using a 0.01 amu mass window. The advantage of a TOF analyzer for product ion data is that the whole MSMS spectrum is collected at high mass accuracy and MRM experiments are conducted in silico after the analysis.
authors: The Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA.
authors: Landon
authors: L
authors: Wilson
authors: The Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA.
authors: Ali
authors: A
authors: Arabshahi
authors: AB Sciex, Concord, Ontario, Canada.
authors: Brigitte
authors: B
authors: Simons
authors: Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA; The Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA; The O'Brien Acute Kidney Injury Center, University of Alabama at Birmingham, Birmingham, AL, USA.
authors: Jeevan K
authors: JK
authors: Prasain
authors: Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA; The Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA; The O'Brien Acute Kidney Injury Center, University of Alabama at Birmingham, Birmingham, AL, USA. Electronic address: sbarnes@uab.edu.
authors: Stephen
authors: S
authors: Barnes
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D036103:Nanotechnology / Q000379:methods*; D059808:Polyphenols / Q000032:analysis* / Q000097:blood / Q000378:metabolism* / Q000652:urine
chemical_list: D059808:Polyphenols
keywords: Mass accuracy; Nanoliquid chromatography–mass spectrometry; Polyphenols; Quantification; Sample volumes; Sensitivity