title: Performance and microbial community profiles in an anaerobic reactor treating with simulated PTA wastewater: from mesophilic to thermophilic temperature.
abstract: Performance and microbial community profiles in a hybrid anaerobic reactor treating synthetic PTA wastewater (contained the major pollutants terephthalate and benzoate) were studied over 220 days from 33 °C to 52 °C. Results indicated that PTA treatment process was highly sensitive to temperature variations in terms of COD removal. Operation at 37 °C showed the best performance as well as the most diverse microbial community revealed by 16S rRNA gene clone library and T-RFLP (terminal restriction fragment length polymorphism). Finally, the anaerobic process achieved a total COD removal of 77.4%, 91.9%, 87.4% and 66.1% at 33, 37, 43 and 52 °C. While the corresponding TA removal were 77.6%, 94.0%, 89.1% and 60.8%, respectively. Sequence analyses revealed acetoclastic Methanosaeta was preponderant at 37 °C, while hydrogenotrophic genera including Methanobrevibacter and Methanofollis were more abundant at other temperatures. For bacterial community, 16 classes were identified. The largely existent Syntrophorhabdus members (belonging to δ-Proteobacteria) at 37 °C was likely to play an important role in mesophilic anaerobic wastewater treatment system contained terephthalate. Meanwhile, β-Proteobacteria seemed to be favored in an anaerobic system higher than 43 °C.
authors: School of Municipal and Environmental Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, China. Electronic address: xkli312@163.com.
authors: Xiang-kun
authors: XK
authors: Li
authors: School of Municipal and Environmental Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, China.
authors: Kai-li
authors: KL
authors: Ma
authors: School of Municipal and Environmental Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, China.
authors: Ling-wei
authors: LW
authors: Meng
authors: School of Municipal and Environmental Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, China.
authors: Jie
authors: J
authors: Zhang
authors: School of Municipal and Environmental Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, China. Electronic address: hitwk@sina.com.
authors: Ke
authors: K
authors: Wang
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010795:Phthalic Acids / Q000378:metabolism*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D062065:Wastewater / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D010795:Phthalic Acids; D012336:RNA, Ribosomal, 16S; D062065:Wastewater; D014874:Water Pollutants, Chemical; C011363:terephthalic acid
keywords: Microbial community; PTA wastewater; Phylogenetic analysis; T-RFLP; Temperature influence