title: In vitro antibiofilm activity of bioactive glass S53P4.
abstract: OBJECTIVE
  This work aimed to investigate the ability of different formulations of bioactive glass (BAG)-S53P4 to interfere with bacterial biofilm produced on prosthetic material by methicillin-resistant Staphylococcus aureus and multi-drug-resistant Pseudomonas aeruginosa.
  
  
  METHODS
  Antibiofilm activity of three formulations of bioglass was assessed at different time points through two different analyses: Crystal Violet and confocal laser scanning microscopy assays.
  
  
  RESULTS
  Significant differences in the whole biofilm were observed between BAG-S53P4-treated and control samples, while no marked changes in antibiofilm activity were observed among the tested formulations. Data from colorimetric assay were confirmed by confocal laser scanning microscopy analysis, which evidenced the significant reduction in biomass and a decrease of total cell volume when both S. aureus and P. aeruginosa biofilms were treated with BAG-S53P4.
  
  
  CONCLUSIONS
  BAG-S53P4 can be considered as an excellent adjuvant in the treatment of prosthetic infections related to biofilm.
authors: Laboratory of Clinical Chemistry & Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161 Milan, Italy.
authors: Lorenzo
authors: L
authors: Drago
authors: Christian
authors: C
authors: Vassena
authors: Simone
authors: S
authors: Fenu
authors: Elena
authors: E
authors: De Vecchi
authors: Valentina
authors: V
authors: Signori
authors: Raffaele
authors: R
authors: De Francesco
authors: Carlo Luca
authors: CL
authors: Romanò
mesh_terms: D000900:Anti-Bacterial Agents; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D018533:Biomass; D024901:Drug Resistance, Multiple, Bacterial; D005898:Glass / Q000737:chemistry*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D019736:Prostheses and Implants / Q000382:microbiology*; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000517:prevention & control; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000517:prevention & control
chemical_list: D000900:Anti-Bacterial Agents; C403922:bioactive glass S53P4
keywords: bacterial biofilm; bioactive glass; confocal laser scanning microscopy; prosthetic joint infections; prosthetic materials