title: Anti-hepatoma effect of safrole from Cinnamomum longepaniculatum leaf essential oil in vitro.
abstract: The aim of this study was to study the anti-hepatoma effect of safrole and elucidate its molecular mechanism, the human hepatoma BEL-7402 cells were incubated with various concentrations (40, 80, 160, 320 and 640 μg/ml) of safrole and the cell proliferation and apoptosis were evaluated. The results showed that both the cell proliferation determined by 3-(4,5-dimethyl-thiazolyl-2)-2,5-diphenyl tetrazolium brominde (MTT) assay and cell colony determined by soft agar assay were significantly suppressed by safrole in a dose-time-dependent manner. Characteristic morphological and biochemical changes associated with apoptosis, including cells shrinkage, deformation and vacuolization of mitochondria, nuclear chromatin condensation and fragmentation, formation of apoptotic bodies were observed when treated with safrole for 24 h and 48 h. Cell cycle changes evaluated by flow cytometry analysis showed that the safrole could induce accumulation of cells arrested at G1 and S phases of the cell cycle. These results demonstrated that safrole is potent anti-hepatoma agent and the underlying mechanism may be attributed to suppress tumor cell growth by inducing cell apoptosis.
authors: College of Veterinary Medicine, Sichuan Agricultural University Ya-an, 625014, China.
authors: Xu
authors: X
authors: Song
authors: College of Veterinary Medicine, Sichuan Agricultural University Ya-an, 625014, China.
authors: Zhongqiong
authors: Z
authors: Yin
authors: College of Veterinary Medicine, Sichuan Agricultural University Ya-an, 625014, China.
authors: Kuichuan
authors: K
authors: Ye
authors: Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education, Yibin University Yibin, Sichuan 644000, China.
authors: Qin
authors: Q
authors: Wei
authors: College of Veterinary Medicine, Sichuan Agricultural University Ya-an, 625014, China.
authors: Renrong
authors: R
authors: Jia
authors: Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education, Yibin University Yibin, Sichuan 644000, China.
authors: Lijun
authors: L
authors: Zhou
authors: Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education, Yibin University Yibin, Sichuan 644000, China.
authors: Yonghua
authors: Y
authors: Du
authors: College of Veterinary Medicine, Sichuan Agricultural University Ya-an, 625014, China.
authors: Jiao
authors: J
authors: Xu
authors: College of Veterinary Medicine, Sichuan Agricultural University Ya-an, 625014, China.
authors: Xiaoxia
authors: X
authors: Liang
authors: College of Veterinary Medicine, Sichuan Agricultural University Ya-an, 625014, China.
authors: Changliang
authors: C
authors: He
authors: College of Veterinary Medicine, Sichuan Agricultural University Ya-an, 625014, China.
authors: Gang
authors: G
authors: Shu
authors: College of Veterinary Medicine, Sichuan Agricultural University Ya-an, 625014, China.
authors: Lizi
authors: L
authors: Yin
authors: College of Veterinary Medicine, Sichuan Agricultural University Ya-an, 625014, China.
authors: Cheng
authors: C
authors: Lv
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D027424:Cinnamomum / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008928:Mitochondria / Q000187:drug effects / Q000473:pathology; D009822:Oils, Volatile / Q000737:chemistry*; D008517:Phytotherapy; D018515:Plant Leaves; D010938:Plant Oils / Q000737:chemistry*; D010946:Plants, Medicinal; D059807:S Phase Cell Cycle Checkpoints / Q000187:drug effects; D012451:Safrole / Q000302:isolation & purification / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D009822:Oils, Volatile; D010938:Plant Oils; D012451:Safrole
keywords: Cinnamomum longepaniculatum; Safrole; apoptosis; cell proliferation; human hepatoma