title: Significant association of Interleukin-10 genotypes and oral cancer susceptibility in Taiwan.
abstract: Interleukin-10 (IL10) is an immunosuppressive cytokine which may facilitate carcinogenesis by down-regulating interferon-gamma production and supporting tumor escape from the immune response. Polymorphisms within the promoter of IL10 gene may not only contribute to differential IL10 expression levels among individuals but also to oral cancer susceptibility. In this hospital-based study, the association of IL10 A-1082G (rs1800896), T-819C (rs3021097), and A-592C (rs1800872) polymorphisms with oral cancer risk were examined. A total of 788 cases with oral cancer risk and 956 controls were genotypes and analyzed by polymerase chain reaction and restriction fragment length polymorphism. The results showed that there were significant differential distributions among oral cancer cases and controls in the genotypic (p=6.29×10(-11)) and allelic (p=2.80×10(-13)) frequencies of IL10 A-1082G. Individuals who carried the AG or GG genotype for IL10 A-1082G had a 1.90- and 3.27-fold higher risk, respectively, of developing nasopharyngeal carcinoma compared to those who carried AA genotype (95% confidence interval=1.51-2.39 and 1.95-5.47). None of the other two polymorphisms investigated appear to affect cancer risk. In gene-lifestyle interaction analysis, we provide first evidence showing of an obvious joint effect of IL10 A-1082G genotype with individual smoking and areca chewing habits on nasopharyngeal carcinoma risk. The AG and GG genotypes of IL10 A-1082G, together with smoking and areca chewing habits, synergistically contribute to individual susceptibility for oral cancer.
authors: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, R.O.C. Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Chia-Wen
authors: CW
authors: Tsai
authors: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Wen-Shin
authors: WS
authors: Chang
authors: Department of Clinical Nutrition, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Kuo-Cheng
authors: KC
authors: Lin
authors: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. Department of Otolaryngology, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Liang-Chun
authors: LC
authors: Shih
authors: Department of Otolaryngology, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Ming-Hsui
authors: MH
authors: Tsai
authors: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Chieh-Lun
authors: CL
authors: Hsiao
authors: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Mei-Due
authors: MD
authors: Yang
authors: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. datian@mail.cmuh.org.tw artbau2@gmail.com.
authors: Cheng-Chieh
authors: CC
authors: Lin
authors: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, R.O.C. Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C. datian@mail.cmuh.org.tw artbau2@gmail.com.
authors: Da-Tian
authors: DT
authors: Bau
mesh_terms: D002277:Carcinoma; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000276:immunology; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000276:immunology; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D013624:Taiwan
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10
keywords: Carcinogenesis; IL10; oral cancer; polymorphism