title: [Determination of ochratoxin A in human urine by HPLC-FLD after cleaned-up by molecularly imprinted polymer solid phase extraction column].
abstract: A method was developed for the determination of ochratoxin A (OTA) in human urine by HPLC-FLD after molecularly imprinted polymer solid phase extraction (MIP-SPE) column. After the pH being adjusted to 2.5 with 0.1 mol x L(-1) HC1, sample was cleaned up with MIP-SPE column for ochratoxin A, the analyte was analyzed by high performance liquid chromatography coupled with fluorescence detection (HPLC-FLD), and finally all the positive results were confirmed by LC-MS/MS. Recoveries from urine samples spiked with OTA at levels ranging from 2 to 20 ng x mL(-1) were 90.6%-101.9%, and RSDs were 0.1%-1.6%. Sixty-five volunteers living in Beijing took part in the study, of which 5 were found containing OTA in their urine and the highest value was 0.091 ng x mL(-1). The MIP-SPE column was firstly applied to purify and concentrate OTA in human urine, this method is simple, rapid and reliable and can be used to determine the contents of OTA in human urine.
authors: Li-Wei
authors: LW
authors: Xie
authors: Xiang-Sheng
authors: XS
authors: Zhao
authors: Wei-Jun
authors: WJ
authors: Kong
authors: Yu-Ting
authors: YT
authors: Wang
authors: Yi-Chen
authors: YC
authors: Hu
authors: Zhen
authors: Z
authors: Ou-Yang
authors: Mei-Hua
authors: MH
authors: Yang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D054802:Molecular Imprinting; D009793:Ochratoxins / Q000652:urine*; D011108:Polymers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction
chemical_list: D009793:Ochratoxins; D011108:Polymers; C025589:ochratoxin A