title: Biofuel cells based on direct enzyme-electrode contacts using PQQ-dependent glucose dehydrogenase/bilirubin oxidase and modified carbon nanotube materials.
abstract: Two types of carbon nanotube electrodes (1) buckypaper (BP) and (2) vertically aligned carbon nanotubes (vaCNT) have been used for elaboration of glucose/O2 enzymatic fuel cells exploiting direct electron transfer. For the anode pyrroloquinoline quinone dependent glucose dehydrogenase ((PQQ)GDH) has been immobilized on [poly(3-aminobenzoic acid-co-2-methoxyaniline-5-sulfonic acid), PABMSA]-modified electrodes. For the cathode bilirubin oxidase (BOD) has been immobilized on PQQ-modified electrodes. PABMSA and PQQ act as promoter for enzyme bioelectrocatalysis. The voltammetric characterization of each electrode shows current densities in the range of 0.7-1.3 mA/cm(2). The BP-based fuel cell exhibits maximal power density of about 107 µW/cm(2) (at 490 mV). The vaCNT-based fuel cell achieves a maximal power density of 122 µW/cm(2) (at 540 mV). Even after three days and several runs of load a power density over 110 µW/cm(2) is retained with the second system (10mM glucose). Due to a better power exhibition and an enhanced stability of the vaCNT-based fuel cells they have been studied in human serum samples and a maximal power density of 41 µW/cm(2) (390 mV) can be achieved.
authors: Biosystems Technology, Technical University of Applied Sciences, 15745 Wildau, Germany.
authors: V
authors: V
authors: Scherbahn
authors: Biosystems Technology, Technical University of Applied Sciences, 15745 Wildau, Germany.
authors: M T
authors: MT
authors: Putze
authors: Institute for Thin Film and Microsensoric Technology, 14513 Teltow, Germany.
authors: B
authors: B
authors: Dietzel
authors: Technical University Darmstadt, Eduard-Zintl-Institute for Inorganic and Physical Chemistry, 64287 Darmstadt, Germany.
authors: T
authors: T
authors: Heinlein
authors: Technical University Darmstadt, Eduard-Zintl-Institute for Inorganic and Physical Chemistry, 64287 Darmstadt, Germany.
authors: J J
authors: JJ
authors: Schneider
authors: Biosystems Technology, Technical University of Applied Sciences, 15745 Wildau, Germany. Electronic address: flisdat@th-wildau.de.
authors: F
authors: F
authors: Lisdat
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000201:enzymology*; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D015374:Biosensing Techniques / Q000295:instrumentation; D060026:Endophytes / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000648:ultrastructure; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000378:metabolism*; D045542:PQQ Cofactor / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D045542:PQQ Cofactor; D042843:Glucose 1-Dehydrogenase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D005947:Glucose
keywords: Bilirubin oxidase; Buckypaper; Enzymatic fuel cell; PQQ-dependent glucose dehydrogenase; Vertically aligned carbon nanotubes