title: Liquid chromatography with diode array detection and multivariate curve resolution for the selective and sensitive quantification of estrogens in natural waters.
abstract: Following the green analytical chemistry principles, an efficient strategy involving second-order data provided by liquid chromatography (LC) with diode array detection (DAD) was applied for the simultaneous determination of estriol, 17β-estradiol, 17α-ethinylestradiol and estrone in natural water samples. After a simple pre-concentration step, LC-DAD matrix data were rapidly obtained (in less than 5 min) with a chromatographic system operating isocratically. Applying a second-order calibration algorithm based on multivariate curve resolution with alternating least-squares (MCR-ALS), successful resolution was achieved in the presence of sample constituents that strongly coelute with the analytes. The flexibility of this multivariate model allowed the quantification of the four estrogens in tap, mineral, underground and river water samples. Limits of detection in the range between 3 and 13 ng L(-1), and relative prediction errors from 2 to 11% were achieved.
authors: Instituto de Química Rosario (CONICET-UNR), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531 (2000), Rosario, Argentina.
authors: Rocío L
authors: RL
authors: Pérez
authors: Instituto de Química Rosario (CONICET-UNR), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531 (2000), Rosario, Argentina. Electronic address: escandar@iquir-conicet.gov.ar.
authors: Graciela M
authors: GM
authors: Escandar
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D060766:Drinking Water / Q000737:chemistry*; D004967:Estrogens / Q000032:analysis*; D060587:Groundwater / Q000737:chemistry*; D008900:Mineral Waters / Q000032:analysis*; D045483:Rivers / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D060766:Drinking Water; D004967:Estrogens; D008900:Mineral Waters; D014874:Water Pollutants, Chemical
keywords: Diode array detection; Estrogens; Liquid chromatography; Multivariate curve resolution; Natural waters