title: [Effect of activating Toll-like receptor 4 on renin-angiotensin system in 3T3-L1 adipose cells].
abstract: OBJECTIVE
  To investigate the role of Toll-like receptor 4 (TLR4) signaling pathway in the activation of rennin- angiotensin system (RAS) in adipose cells.
  
  
  METHODS
  3T3-L1 cells induced with isobutylmethylxanthine, insulin and dexamethasone to differentiate into adipocytes were stimulated by LPS with or without irbesartan pretreatment. The expression levels of TLR4, angiotensinogen (AGT) and angiotensin II receptor type 1 (ATlR) mRNA in 3T3-L1 cells was determined by RT-PCR, and their protein expressions were detected with Western-blotting. Immunofluorescence double staining was used to observe the translocation of NF-κB p65 subunit in the cells.
  
  
  RESULTS
  Stimulation with LPS dose- and time-dependently increased the mRNA and protein expressions of TLR4, AGT and AT1R. LPS exposure resulted in enhanced translocation of NF-κB p65 subunit in the adipose cells, which was attenuated by irbesartan pretreatment.
  
  
  CONCLUSIONS
  Activation of TLR4 signaling pathway may trigger the activation of local RAS in adipose cells.
authors: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.E-mail: realbabe@163.com.
authors: Jinhua
authors: J
authors: Luo
authors: Jia
authors: J
authors: Sun
authors: Dehong
authors: D
authors: Cai
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000808:Angiotensinogen / Q000378:metabolism; D000818:Animals; D001713:Biphenyl Compounds / Q000494:pharmacology; D000077405:Irbesartan; D051379:Mice; D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism; D012084:Renin-Angiotensin System; D015398:Signal Transduction; D013777:Tetrazoles / Q000494:pharmacology; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D001713:Biphenyl Compounds; D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; C498144:Rela protein, mouse; D013777:Tetrazoles; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D051996:Transcription Factor RelA; D000808:Angiotensinogen; D000077405:Irbesartan