title: Evaluation of ebselen supplementation on cryopreservation medium in human semen.
abstract: BACKGROUND
  An effect of cryopreservation on human sperm is sublethal cryodamage, in which cell viability post-thaw is lost more rapidly at later times than in fresh cells.
  
  
  OBJECTIVE
  This study examined whether the addition of an antioxidant to cryopreservation medium could improve the post-thaw parameters and evaluation of sperm chromatin quality of cryopreserved human spermatozoa from men with normal semen parameters.
  
  
  METHODS
  Semen samples (n=35) were collected by masturbation and assessed following WHO standards. Individual samples were classified as two portions. One portion (n=10) was for elucidate the concentration of ebselen.Then the samples(n=25) were divided in to 5groups.The first aliquot remained fresh.The second aliquots was mixed with cryopreservation medium.The third aliquots were mixed with cryopreservation medium containing solvent of ebselen.The forth and fifth aliquots were mixed with cryopreservation medium containing 1.25 and 2.5 µm of ebselen.Samples were frozen and thawed samples were assessed for sperm parameters.Three-way ANOVA Multivariate measures were used to assess. According to this assesment the differences are observed in existent groups in post-thaw count, motility index, vitality staining, and morphology and DNA fragmentation.
  
  
  RESULTS
  After freezing the media containing of ebselen, DNA fragmentation is significantly different in comparison with control group. ebselen with 1.25 µm dose was significantly associated with post-thaw DNA fragmentation (p=0.047). Similarly ebselen with 2.5 µm dose was significantly associated with post-thaw DNA fragmentation (p=0.038). But other parameters were not altered.
  
  
  CONCLUSIONS
  These results suggest that the addition of ebselen to cryopreservation medium doesnot improve post-thaw parameters and DNA fragmentation of sperm.
authors: Kashan Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors: Zohreh
authors: Z
authors: Khodayari Naeini
authors: Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors: Hassan
authors: H
authors: Hassani Bafrani
authors: Kashan Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors: Hossein
authors: H
authors: Nikzad
keywords: Cryopreservation; DNA fragmentation; Ebselen; Human sperm