title: A case of cervical cancer expressed three mRNA variant of Hyaluronan-mediated motility receptor.
abstract: Cervical cancer is the second malignancy in Mexico, little is known about the prognostic factors associated with this disease. Several cellular components are important in their transformation and progression. Alternative mRNA splice is an important mechanism for generating protein diversity, nevertheless, in cancer unknown mRNA diversity is expressed. Hyaluronan-mediated motility receptor (HMMR, RHAMM, CD168) is a family member of proteins, hyaluronan acid dependent, and has been associated with different malignant processes such as: angiogenesis, cell invasiveness, proliferation, metastasis and poor outcome in some tumors. In the present study we identified expression of HMMR in cervical cancer by means of RT-PCR and sequencing. Our results indicate co-expression of two HMMR variants in all samples, and one case expressed three alternative HMMR splice transcripts. These results showed the heterogeneity of mRNA transcripts of HMMR that could express in cancer and the expression of HMMR could be marker of malignancy in CC.
authors: Genomic Oncology Laboratory, Medical Research Unit in Oncologic Diseases, Oncology Hospital, National Medical Center Siglo XXI, IMSS México, D.F., México.
authors: Vanessa
authors: V
authors: Villegas-Ruíz
authors: Genomic Oncology Laboratory, Medical Research Unit in Oncologic Diseases, Oncology Hospital, National Medical Center Siglo XXI, IMSS México, D.F., México.
authors: Mauricio
authors: M
authors: Salcedo
authors: Department of Medical Genomics and Environmental Toxicology, Biomedical Research Institute, UNAM México, D.F., México ; Biochemistry Unit, National Institute of Medical Sciences and Nutrition "Salvador Zubirán" México, D.F., México.
authors: Alejandro
authors: A
authors: Zentella-Dehesa
authors: Biochemistry Unit, National Institute of Medical Sciences and Nutrition "Salvador Zubirán" México, D.F., México.
authors: Edén V Montes
authors: EV
authors: de Oca
authors: Department of Oncology, General Hospital of México O. D México, D.F., México.
authors: Edgar
authors: E
authors: Román-Basaure
authors: Department of Pathology, National Medical Center Siglo XXI, IMSS México, D.F., México.
authors: Alejandra
authors: A
authors: Mantilla-Morales
authors: Experimental Oncology Laboratory, Department of Research, National Institute of Pediatrics México, D.F., México.
authors: Víctor M
authors: VM
authors: Dávila-Borja
authors: Experimental Oncology Laboratory, Department of Research, National Institute of Pediatrics México, D.F., México.
authors: Sergio
authors: S
authors: Juárez-Méndez
mesh_terms: D017398:Alternative Splicing; D001483:Base Sequence; D005091:Exons; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D014644:Genetic Variation; D006367:HeLa Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D016326:Extracellular Matrix Proteins; D018960:Hyaluronan Receptors; D012333:RNA, Messenger; C098946:hyaluronan-mediated motility receptor
keywords: Cervical cancer; HMMR; alternative splicing; expression