title: Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics.
abstract: We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport.
authors: Department of Electrical and Computer Engineering, USA. wshih@uh.edu.
authors: Ji
authors: J
authors: Qi
authors: Jianbo
authors: J
authors: Zeng
authors: Fusheng
authors: F
authors: Zhao
authors: Steven Hsesheng
authors: SH
authors: Lin
authors: Balakrishnan
authors: B
authors: Raja
authors: Ulrich
authors: U
authors: Strych
authors: Richard C
authors: RC
authors: Willson
authors: Wei-Chuan
authors: WC
authors: Shih
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis / Q000378:metabolism; D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004058:Diffusion; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization; D016062:Porosity; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D020349:Surface Plasmon Resonance
chemical_list: D002232:Carbocyanines; D004247:DNA; D004277:DNA, Single-Stranded; D006046:Gold; D018719:Erb-b2 Receptor Tyrosine Kinases; C089187:cyanine dye 3; C508053:ERBB2 protein, human