title: The use of cholinesterase as potential biomarker: In vitro characterization in the polychaete Capitella teleta.
abstract: The ecological relevance of polychaetes coupled with their easy culture and maintenance in the laboratory, has led them to become increasingly used in marine ecotoxicological studies, raising the need to validate frequently applied monitoring tools at various biological levels. The present study was aimed to characterize the cholinesterases (ChE) activity in the polychaete Capitella teleta, using three substrates (acetylthiocholine iodide, propionylthiocholine iodide, and S-butyrylthiocholine iodide) and four known inhibitors (eserine hemisulfate, BW284c51, iso-OMPA and chlorpyrifos-oxon). Results showed that most of the measured cholinesterase activity was acetylcholinesterase (AChE). Inhibition of enzyme kinetic experiments denoted that sensitivity of C. teleta's ChE to the organophosphorous metabolite chlorpyrifos-oxon (IC₅₀=60.72 nM) was analogous to some fish species. This study highlights the relevance of ChE characterization before its use as a biomarker in ecotoxicology and biomonitoring studies.
authors: Environmental Chemistry Department, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain; Marine Resources Research Group, ESTM & GIRM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal.
authors: Isa D L
authors: ID
authors: Gomes
authors: Marine Resources Research Group, ESTM & GIRM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal.
authors: Marco F L
authors: MF
authors: Lemos
authors: CESAM & Dept. Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
authors: Amadeu M V M
authors: AM
authors: Soares
authors: Environmental Chemistry Department, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
authors: Carlos
authors: C
authors: Barata
authors: CESAM & Dept. Biology, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: mpfaria@ua.pt.
authors: Melissa
authors: M
authors: Faria
mesh_terms: D000122:Acetylthiocholine / Q000031:analogs & derivatives / Q000032:analysis; D000818:Animals; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide / Q000032:analysis; D015415:Biomarkers / Q000378:metabolism*; D002092:Butyrylthiocholine / Q000032:analysis; D004390:Chlorpyrifos / Q000031:analogs & derivatives / Q000032:analysis; D002800:Cholinesterase Inhibitors; D002802:Cholinesterases / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods; D020128:Inhibitory Concentration 50; D010830:Physostigmine / Q000032:analysis; D011077:Polychaeta / Q000187:drug effects* / Q000378:metabolism; D013431:Sulfates / Q000032:analysis; D013770:Tetraisopropylpyrophosphamide / Q000032:analysis; D013860:Thiocholine / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D015415:Biomarkers; D002800:Cholinesterase Inhibitors; D013431:Sulfates; C543539:acetylthiocholine iodide; C009063:propionylthiocholine; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide; D000122:Acetylthiocholine; D002092:Butyrylthiocholine; D013770:Tetraisopropylpyrophosphamide; C009618:O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate; D013860:Thiocholine; D010830:Physostigmine; D002802:Cholinesterases; D004390:Chlorpyrifos
keywords: Acetylcholinesterase; Capitela teleta; Insecticide; Marine; Organophosphorous; Sediment