title: New construction of an animal model for the orthotopic transplantation of an ovarian tumor.
abstract: A new technique has successfully established the non-obese diabetic/severely combined immunodeficiency (NOD/SCID) mouse model of ovarian cancer. Under 4% chloral hydrate (0.1 mL/g dose) anesthesia, female mice were inoculated with tumor-cell suspension. The expression rate of OVCAR3 to CA125 was assessed using flow cytometry. The inoculated site was hand palpated and the signs and symptoms related to tumor growth were observed with the naked eye. The allophycocyanin (APC) indirectly labeled mouse-antihuman CA125 and fluorescein isothiocyanate (FITC)-labeled anti-mouse MHC Class I molecule (H-2K(d)/H-2D(d)) were observed using a confocal laser scanning microscope. The animal model of ovarian cancer constructed using this method can more directly reflect the characteristics of cancer cells. It provides reliable experimental results and presents a technical platform for the research of ovarian cancer stem cells.
authors: Affiliated Hospital Of HeBei University, Baoding City, HeBei Province, China.
authors: Hui
authors: H
authors: Zhang
authors: Affiliated Hospital Of HeBei University, Baoding City, HeBei Province, China.
authors: Xinping
authors: X
authors: Gao
authors: The Third Hospital Of Baoding/Baoding Cancer Hospital, Baoding City, HeBei Province, China.
authors: Yongan
authors: Y
authors: Yang
authors: Affiliated Hospital Of HeBei University, Baoding City, HeBei Province, China.
authors: Weiming
authors: W
authors: Wang
authors: Affiliated Hospital Of HeBei University, Baoding City, HeBei Province, China.
authors: Jin
authors: J
authors: Liu
authors: Affiliated Hospital Of HeBei University, Baoding City, HeBei Province, China.
authors: Yijuan
authors: Y
authors: Liang
authors: Affiliated Hospital Of HeBei University, Baoding City, HeBei Province, China.
authors: Hongli
authors: H
authors: Wu
authors: Affiliated Hospital Of HeBei University, Baoding City, HeBei Province, China.
authors: Jinjin
authors: J
authors: Qin
authors: Affiliated Hospital Of HeBei University, Baoding City, HeBei Province, China.
authors: Kun
authors: K
authors: Pan
authors: Department of Obstetrics and Gynecology, ZhuJiang Hospital of Southem Medical University, Guangzhou City, Guangdong Province, China.
authors: Yifeng
authors: Y
authors: Wang
authors: Affiliated Hospital Of HeBei University, Baoding City, HeBei Province, China.
authors: Junrong
authors: J
authors: Shi
authors: Affiliated Hospital Of HeBei University, Baoding City, HeBei Province, China.
authors: Youju
authors: Y
authors: Ma
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology; D010051:Ovarian Neoplasms / Q000473:pathology*
keywords: Animal model; Orthotopic transplantation; Ovarian cancer; Tumor