title: [Celecoxib antagonizes the cytotoxic effect of carboplatin in human esophageal cancer cells].
abstract: OBJECTIVE
  To explore the antagonizing effect of celecoxib against the cytotoxicity of carboplatin in human esophageal cancer cells.
  
  
  METHODS
  The cell viability of cisplatin-resistant cell line EC109/CDDP and its parental cell line EC109 exposed to carboplatin alone or carboplatin plus celecoxib was determined by MTT assay. The expression of CTR1, caspase-3 activation and PARP cleavage in the exposed cells were examined by Western blotting. Caspase-3 activity and cell apoptosis after the exposure were detected with Caspase-3/7 assay and flow cytometry, respectively. The effect of celecoxib on carboplatin accumulation in the cells was measured using inductively coupled plasma mass spectrometry (ICP-MS).
  
  
  RESULTS
  Celecoxib treatment significantly increased the IC50 of carboplatin, suppressed carboplatin-induced caspase-3 and PARP cleavage and caspase-3 activity in EC109 and EC109/CDDP cells. Celecoxib also inhibited carboplatin-induced apoptosis and suppressed intracellular carboplatin accumulation in both cell lines. A combined exposure to celecoxib and carboplatin did not cause significant changes in the protein expression of CTR1.
  
  
  CONCLUSIONS
  Celecoxib antagonizes the cytotoxic effect of carboplatin and inhibits carboplatin-induced apoptosis in human esophageal cancer cells by reducing intracellular carboplatin accumulation.
authors: School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.E-mail: 727654480@qq.com.
authors: Lili
authors: L
authors: Shi
authors: Desheng
authors: D
authors: Zhong
authors: Chunping
authors: C
authors: Gu
authors: Le
authors: L
authors: Yu
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D016190:Carboplatin / Q000037:antagonists & inhibitors*; D053148:Caspase 3 / Q000378:metabolism; D000068579:Celecoxib; D045744:Cell Line, Tumor / Q000187:drug effects; D002470:Cell Survival; D004347:Drug Interactions; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D011720:Pyrazoles / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*
chemical_list: D011720:Pyrazoles; D013449:Sulfonamides; D016190:Carboplatin; C505407:CASP3 protein, human; D053148:Caspase 3; D000068579:Celecoxib