title: Photophysical and calorimetric investigation on the structural reorganization of poly(A) by phenothiazinium dyes azure A and azure B.
abstract: Poly(A) has significant relevance to mRNA stability, protein synthesis and cancer biology. The ability of two phenothiazinium dyes azure A (AA) and azure B (AB) to bind single-stranded poly(A) was studied by spectroscopic and calorimetric techniques. Strong binding of the dyes and the higher affinity of AA over AB were ascertained from absorbance and fluorescence experiments. Significant perturbation of the circular dichroism spectrum of poly(A) in the presence of these molecules with formation of induced CD bands in the 300-700 nm region was observed. Strong emission polarization of the bound dyes and strong energy transfer from the adenine base pairs of poly(A) suggested intercalative binding to poly(A). Intercalative binding was confirmed from fluorescence quenching experiments and was predominantly entropy driven as evidenced from isothermal titration calorimetry data. The negative values of heat capacity indicated involvement of hydrophobic forces and enthalpy-entropy compensation suggested noncovalent interactions in the complexation for both the dyes. Poly(A) formed a self-assembled structure on the binding of both the dyes that was more favored under higher salt conditions. New insights in terms of spectroscopic and thermodynamic aspects into the self-structure formation of poly(A) by two new phenothiazinium dyes that may lead to structural and functional damage of mRNA are revealed from these studies.
authors: Biophysical Chemistry Laboratory, Chemisry Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700 032, India. gskumar@iicb.res.in gsk.iicb@gmail.com.
authors: Puja
authors: P
authors: Paul
authors: Gopinatha Suresh
authors: GS
authors: Kumar
mesh_terms: D001399:Azure Stains / Q000737:chemistry / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D004735:Energy Transfer; D005454:Fluorescence Polarization; D006801:Humans; D015394:Molecular Structure; D009690:Nucleic Acid Conformation / Q000187:drug effects / Q000528:radiation effects; D055668:Photochemical Processes; D011061:Poly A / Q000737:chemistry* / Q000528:radiation effects*; D020871:RNA Stability / Q000187:drug effects / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: D001399:Azure Stains; D011061:Poly A; C025819:Azure B; C025818:Azure A