title: Serum microRNA expression signatures identified from genome-wide microRNA profiling serve as novel noninvasive biomarkers for diagnosis and recurrence of bladder cancer.
abstract: Recent advantages of serum microRNAs (miRNAs) open a new realm of possibilities for noninvasive diagnosis and prognosis of bladder cancer (BC). The aim of our study was to identify serum miRNA expression signatures in patients with BC and establish new models for the diagnosis of BC and recurrence prediction. We performed genome-wide serum miRNA analysis by Miseq sequencing followed by evaluations in the training and validation sets with reverse transcription quantitative real-time PCR assays from serum samples of 250 patients with BC and 240 controls. A six-miRNA panel (miR-152, miR-148b-3p, miR-3187-3p, miR-15b-5p, miR-27a-3p and miR-30a-5p) for the diagnosis of BC was finally developed by multivariate logistic regression model with an area under the receiver operating characteristic curve of 0.899. The corresponding sensitivities of this panel for Ta, T1 and T2-T4 were 90.00, 84.85 and 89.36%, significantly higher than those of urine cytology, which were 13.33, 30.30 and 44.68%, respectively (all at p<0.001). In addition, Kaplan-Meier analysis showed that patients with nonmuscle-invasive BC (NMIBC) with high miR-152 level and low miR-3187-3p level had worse recurrence-free survival (p=0.023 and 0.043, respectively). In multivariate Cox regression analysis, miR-152 was independently associated with tumor recurrence of NMIBC (p=0.028). Our results suggested that a serum miRNA signature may have considerable clinical value in diagnosing BC. Furthermore, expression level of serum miR-152 could provide information on the recurrence risk of NMIBC.
authors: Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shandong Province, China.
authors: Xiumei
authors: X
authors: Jiang
authors: Lutao
authors: L
authors: Du
authors: Lili
authors: L
authors: Wang
authors: Juan
authors: J
authors: Li
authors: Yimin
authors: Y
authors: Liu
authors: Guixi
authors: G
authors: Zheng
authors: Ailin
authors: A
authors: Qu
authors: Xin
authors: X
authors: Zhang
authors: Hongwei
authors: H
authors: Pan
authors: Yongmei
authors: Y
authors: Yang
authors: Chuanxin
authors: C
authors: Wang
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D015894:Genome, Human; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000097:blood* / Q000235:genetics / Q000401:mortality; D016016:Proportional Hazards Models; D012372:ROC Curve; D059467:Transcriptome; D001749:Urinary Bladder Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000401:mortality
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: bladder cancer; diagnosis; recurrence; serum microRNAs