title: Nanocrystalline diamond surfaces for adhesion and growth of primary neurons, conflicting results and rational explanation.
abstract: Using a variety of proliferating cell types, it was shown that the surface of nanocrystalline diamond (NCD) provides a permissive substrate for cell adhesion and development without the need of complex chemical functionalization prior to cell seeding. In an extensive series of experiments we found that, unlike proliferating cells, post-mitotic primary neurons do not adhere to bare NCD surfaces when cultured in defined medium. These observations raise questions on the potential use of bare NCD as an interfacing layer for neuronal devices. Nevertheless, we also found that classical chemical functionalization methods render the "hostile" bare NCD surfaces with adhesive properties that match those of classically functionalized substrates used extensively in biomedical research and applications. Based on the results, we propose a mechanism that accounts for the conflicting results; which on one hand claim that un-functionalized NCD provides a permissive substrate for cell adhesion and growth, while other reports demonstrate the opposite.
authors: Department of Neurobiology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem Jerusalem, Israel.
authors: Silviya M
authors: SM
authors: Ojovan
authors: Institute for Materials Research in MicroElectronics - Interuniversity Micro Electronics Centre, Hasselt University Antwerp, Belgium.
authors: Matthew
authors: M
authors: McDonald
authors: Mathew
authors: M
authors: McDonald
authors: Department of Neurobiology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem Jerusalem, Israel.
authors: Noha
authors: N
authors: Rabieh
authors: Department of Neurobiology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem Jerusalem, Israel ; The Harvy M. Kruger Family Center for Nanoscience, The Hebrew University of Jerusalem Jerusalem, Israel.
authors: Nava
authors: N
authors: Shmuel
authors: Department of Neurobiology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem Jerusalem, Israel.
authors: Hadas
authors: H
authors: Erez
authors: Institute for Materials Research in MicroElectronics - Interuniversity Micro Electronics Centre, Hasselt University Antwerp, Belgium.
authors: Milos
authors: M
authors: Nesladek
authors: Department of Neurobiology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem Jerusalem, Israel ; The Harvy M. Kruger Family Center for Nanoscience, The Hebrew University of Jerusalem Jerusalem, Israel.
authors: Micha E
authors: ME
authors: Spira
keywords: calcium imaging; cell adhesion; cultured neurons; nanocrystalline diamonds; network connectivity; poly-d-lysine