title: Newborn mice exposed prenatally to bisphenol A show hyperactivity and defective neocortical development.
abstract: The central nervous system is especially susceptible to toxic insults during development. Prenatal administration of bisphenol A (BPA) induces histologic anomalies in the dorsal telencephalon of the embryo. Whether these anomalies affect the morphogenesis and maturation of neuronal function of the newborn neocortex, however, is unknown. To evaluate the neurodevelopmental and behavioral effects of prenatal BPA exposure at 20 and 200μg/kg/day in newborn mice, we performed a detailed histologic analysis of the neocortex and tested for the presence of behavioral abnormalities in newborn mice prenatally exposed to BPA using our newly developed behavioral test. Observations of newborn mice prenatally exposed to BPA revealed abnormal neuronal distribution and layer formation, hypoplasia of layer 6b, and abnormal dopaminergic neuronal projections in the neocortex. Further, the newborn mice exhibited hyperactivity. These findings suggest that prenatal BPA exposure induces neurobehavioral toxicity associated with abnormal dopaminergic neuronal projections, and abnormal corticogenesis and lamination. Histologic and behavioral analyses of newborn mice are considered useful for assessing the neurodevelopmental and behavioral toxicity of chemicals.
authors: Department of Anatomy, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan. Electronic address: komada@dpc.agu.ac.jp.
authors: Munekazu
authors: M
authors: Komada
authors: Department of Life Science, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan. Electronic address: S6a2k9i@gmail.com.
authors: Saki
authors: S
authors: Itoh
authors: Department of Life Science, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan. Electronic address: tpriku11@yahoo.co.jp.
authors: Kota
authors: K
authors: Kawachi
authors: Department of Life Science, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan. Electronic address: kagawa@life.kindai.ac.jp.
authors: Nao
authors: N
authors: Kagawa
authors: Department of Anatomy, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan. Electronic address: yayoi@dpc.agu.ac.jp.
authors: Yayoi
authors: Y
authors: Ikeda
authors: Department of Life Science, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan. Electronic address: tnagao@life.kindai.ac.jp.
authors: Tetsuji
authors: T
authors: Nagao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal / Q000187:drug effects; D001559:Benzhydryl Compounds / Q000633:toxicity*; D059290:Dopaminergic Neurons / Q000187:drug effects; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D006948:Hyperkinesis / Q000139:chemically induced*; D008431:Maternal-Fetal Exchange; D051379:Mice; D008813:Mice, Inbred ICR; D019579:Neocortex / Q000002:abnormalities / Q000187:drug effects*; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Corticogenesis; Neurodevelopmental toxicity; Neuronal projection; Newborn activity