title: Evaluation of vascular grafts based on polyvinyl alcohol cryogels.
abstract: The present study designed and developed blood vessel substitutes (BVSs) composed of polyvinyl alcohol (PVA) cryogels. The in vitro results demonstrated that the coating of the polymer with lyophilized decellularized vascular matrix (DVM) greatly enhanced the adhesion of human umbilical vein endothelial cells (HUVECs). However, when PVA̸DVM BVSs were implanted into the abdominal aorta of Sprague‑Dawley rats, DVM was identified as a highly thrombogenic surface resulting in the mortality of all animals 3‑4 days after surgery. By contrast, all rats implanted with PVA survived and were sacrificed after 12 months. The luminal surface of the explanted grafts was completely covered by endothelial cells and the inner diameter was similar to that of the original vessel. In conclusion, the present study indicated that PVA may be considered as a promising biomaterial for the fabrication of artificial vessels.
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua 35131, Italy.
authors: Maria Teresa
authors: MT
authors: Conconi
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua 35131, Italy.
authors: Luca
authors: L
authors: Borgio
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua 35131, Italy.
authors: Rosa
authors: R
authors: Di Liddo
authors: Department of Plastic and Reconstructive Surgery, University of Padua, Padua 35131, Italy.
authors: Leonardo
authors: L
authors: Sartore
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua 35131, Italy.
authors: Daniele
authors: D
authors: Dalzoppo
authors: Department of Neuroradiology, Hospital of Rovigo, Rovigo 45100, Italy.
authors: Pietro
authors: P
authors: Amistà
authors: Foundation for Biology and Regenerative Medicine, TES‑Tissue Engineering and Signaling (ONLUS), Padua 35131, Italy.
authors: Silvano
authors: S
authors: Lora
authors: Foundation for Biology and Regenerative Medicine, TES‑Tissue Engineering and Signaling (ONLUS), Padua 35131, Italy.
authors: Pier Paolo
authors: PP
authors: Parnigotto
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua 35131, Italy.
authors: Claudio
authors: C
authors: Grandi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D049109:Cell Proliferation; D059985:Cryogels / Q000737:chemistry*; D004730:Endothelium, Vascular / Q000166:cytology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D059985:Cryogels; D011142:Polyvinyl Alcohol