title: Modification and evaluation of Brucella broth based Campylobacter jejuni transport medium.
abstract: Reliable transport of Campylobacter jejuni isolates is critical to microbial epidemiology research, especially in developing countries without a good temperature control mailing system. Various factors, including oxygen, temperature, transport medium composition, could affect the survival of C. jejuni. In this study, the protective effects of different ingredients in C. jejuni transport media at 4 °C and 25 °C and under aerobic condition were quantitatively evaluated respectively. The results showed that enriched medium, supplementation with 5% blood and being kept at 4 °C could improve the viability of different C. jejuni strains during transport. In addition, supplementation with 25 mmol/L L-fucose in Wang's transport medium could significantly improve the survival of C. jejuni at both 4 °C and 25 °C. To the best of our knowledge, this is the first report to evaluate the protective effect of L-fucose in enriched C. jejuni transport medium which is feasible in developing countries without an effective cold chain mailing system. These data will be good reference for C. jejuni transport medium improvement in future.
authors: Key Laboratory of Food Safety Risk Assessment, National Health and Family Planning Commission, China National Center for Food Safety Risk Assessment, Beijing 100021, China; National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors: Yao
authors: Y
authors: Bai
authors: National Institutes for Food and Drug Control, Beijing 100050, China.
authors: Sheng Hui
authors: SH
authors: Cui
authors: National Institutes for Food and Drug Control, Beijing 100050, China.
authors: Xiao
authors: X
authors: Xu
authors: Key Laboratory of Food Safety Risk Assessment, National Health and Family Planning Commission, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors: Feng Qin
authors: FQ
authors: Li
mesh_terms: D001431:Bacteriological Techniques; D016123:Campylobacter jejuni; D003470:Culture Media
chemical_list: D003470:Culture Media