title: A method suitable for DNA extraction from humus-rich soil.
abstract: A rapid and convenient method for extracting DNA from soil is presented. Soil DNA is extracted by direct cell lysis in the presence of EDTA, SDS, phenol, chloroform and isoamyl alcohol (3-methyl-1-butanol) followed by precipitation with 2-propanol. The extracted DNA is purified by modified DNA purification kit and DNA gel extraction kit. With this method, DNA extracted from humus-rich dark brown forest soil was free from humic substances and, therefore, could be used for efficient PCR amplification and restriction digestion. In contrast, DNA sample extracted with the traditional CTAB-based method had lower yield and purity, and no DNA could be extracted from the same soil sample with a commonly-used commercial soil DNA isolation kit. In addition, this method is time-saving and convenient, providing an efficient choice especially for DNA extraction from humus-rich soils.
authors: College of Biosciences and Biotechnology, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, 110161, People's Republic of China.
authors: Tianjin
authors: T
authors: Miao
authors: Song
authors: S
authors: Gao
authors: Shengwei
authors: S
authors: Jiang
authors: Guoshi
authors: G
authors: Kan
authors: Pengju
authors: P
authors: Liu
authors: Xianming
authors: X
authors: Wu
authors: Yingfeng
authors: Y
authors: An
authors: Shuo
authors: S
authors: Yao
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D006812:Humic Substances / Q000382:microbiology*; D054892:Metagenome / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D006812:Humic Substances