title: A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats.
abstract: OBJECTIVE
  To investigate the therapeutic effects of a novel fluid resuscitation protocol (early fluid resuscitation plus 2% hydrogen inhalation) on acute kidney injury during septic shock induced by lipopolysaccharide in rats.
  
  
  METHODS
  Sixty male Wistar rats were randomly divided into four groups (n = 15 per group): control group (C), septic shock group (S), septic shock with early fluid resuscitation group (R), and septic shock with early fluid resuscitation plus 2% hydrogen inhalation group (R+R+H2). The rats were ventilated, and a 2% hydrogen mixture was used in Group R+H2. Lipopolysaccharide (10 mg/kg) was administered to establish the septic shock model in rats and fluid resuscitation was performed in Groups R and R+R+H2.
  
  
  RESULTS
  Fluid resuscitation with 2% hydrogen inhalation decreased serum creatinine, blood urea nitrogen, and neutrophil gelatinase-associated lipocalin. It also reduced oxidative stress injury and decreased renal tumor necrosis factor-α and interleukin-6 levels compared with fluid resuscitation alone.
  
  
  CONCLUSIONS
  Early fluid resuscitation plus 2% hydrogen inhalation provided more protection against AKI during septic shock.
authors: Department of Emergency, First Affiliated Hospital of China Medical University Shenyang, China.
authors: Wei
authors: W
authors: Liu
authors: Department of Emergency, First Affiliated Hospital of China Medical University Shenyang, China.
authors: Xue-Song
authors: XS
authors: Dong
authors: Department of Emergency, First Affiliated Hospital of China Medical University Shenyang, China.
authors: Yu-Qiang
authors: YQ
authors: Sun
authors: Department of Emergency, First Affiliated Hospital of China Medical University Shenyang, China.
authors: Zhi
authors: Z
authors: Liu
keywords: Septic shock; acute kidney injury; fluid resuscitation; hydrogen inhalation; inflammation; oxidative stress