title: Stem-loop DNA-assisted silicon nanowires-based biochemical sensors with ultra-high sensitivity, specificity, and multiplexing capability.
abstract: A class of stem-loop DNA-assisted silicon nanowires (SiNWs)-based fluorescent biosensor is presented in this report. Significantly, the sensor enables rapid and sensitive detection of DNA targets with a concentration as low as 1 pM. Moreover, the large planar surface of SiNWs facilitates simultaneous assembly with different DNA strands, which is favorable for multiplexed DNA detection. On the other hand, the SiNWs-based sensor is highly efficacious for detecting heavy metal ions. Mercury ions (Hg(2+)) of low concentrations (e.g., 5 pM) are readily identified from its mixture with over 10 kinds of interfering metal ions, even in real water samples. Given that SiNWs can be fabricated in a facile, reproducible and low-cost manner, this kind of SiNWs-based high-performance sensor is expected to be a practical analytical tool for a variety of biological and environment-protection applications.
authors: Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, China. yaohe@suda.edu.cn.
authors: Juan
authors: J
authors: Xie
authors: Xiangxu
authors: X
authors: Jiang
authors: Yiling
authors: Y
authors: Zhong
authors: Yimei
authors: Y
authors: Lu
authors: Siyi
authors: S
authors: Wang
authors: Xinpan
authors: X
authors: Wei
authors: Yuanyuan
authors: Y
authors: Su
authors: Yao
authors: Y
authors: He
mesh_terms: D002138:Calibration; D004396:Coloring Agents / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D016147:Genes, Tumor Suppressor; D006801:Humans; D007477:Ions; D007700:Kinetics; D008628:Mercury / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008670:Metals / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D053770:Nanowires / Q000737:chemistry*; D009369:Neoplasms / Q000628:therapy; D009930:Organic Chemicals / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004396:Coloring Agents; D007477:Ions; D008670:Metals; D009930:Organic Chemicals; D004247:DNA; D008628:Mercury; D012825:Silicon