title: Fluorescence detection of DNA hybridization based on the aggregation-induced emission of a perylene-functionalized polymer.
abstract: A perylene-functionalized polycation was synthesized by quaternization of poly(4-vinylpyridine) with bromomethyl-perylene and methyl iodide, which exhibited a unique aggregation-induced emission (AIE) effect. The synthesized polycation and polyanion ssDNA could form a complex probe to detect DNA hybridization. Upon adding noncomplementary ssDNA, the fluorescence of the complex probe increased due to the AIE effect; upon adding complementary ssDNA, the fluorescence intensity changed little due to the combined effects of AIE and duplex-quenching resulting from the intercalation of perylene into the duplex.
authors: School of Materials Science and Engineering, University of Science and Technology Beijing , Beijing 100083, China.
authors: Guojie
authors: G
authors: Wang
authors: Ruichen
authors: R
authors: Zhang
authors: Chen
authors: C
authors: Xu
authors: Ruyi
authors: R
authors: Zhou
authors: Jie
authors: J
authors: Dong
authors: Huitao
authors: H
authors: Bai
authors: Xiaowei
authors: X
authors: Zhan
mesh_terms: D015342:DNA Probes / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000032:analysis*; D005453:Fluorescence; D009693:Nucleic Acid Hybridization / Q000379:methods; D010569:Perylene / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D011145:Polyvinyls; C019535:poly(4-vinylpyridine); D010569:Perylene