title: Neonatal vaccination with plasmid DNA encoding Cyn d 1 effectively prevents allergic responses in mice.
abstract: BACKGROUND
  Allergic rhinitis is one of the most common atopic disorders in children. There is no available method to prevent airway sensitization in newborns except allergen avoidance. Recombinant DNA plasmids encoding allergens have been proven to activate Th1 but attenuate Th2-deviated allergic responses in adult animal studies. However, their preventive effects are not presumptive in neonates because of their immature immune function. The aim of this study was to examine the potential preventive effect of a DNA vaccine encoding grass pollen allergen Cyn d 1 on allergic reaction to grass pollen in neonatal mice.
  
  
  METHODS
  Recombinant plasmid Cyn d 1 (pCyn d 1) vaccine was constructed by insertion of Cyn d 1 cDNA into the vector pcDNA3. Neonatal BALB/c mice received the vaccine once on the 3rd day of life or a second dose 2 days later. Control mice received PBS only. Mice were sensitized twice with recombinant Cyn d 1 and alum beginning at 7 weeks of age. Serum antibody responses and cytokine profiles of spleen cells were examined.
  
  
  RESULTS
  Neonatal injection with pCyn d 1 vaccine resulted in IgG2a responses and production of interferon gamma in spleen cells. Vaccination with pCyn d 1 also reduced specific IgE responses and spleen cell secretion of IL-4.
  
  
  CONCLUSIONS
  This study shows the prophylactic effects of DNA vaccine encoding Bermuda grass pollen allergen Cyn d 1 on specific IgE responses in neonatal mice.
authors: Department of Pediatrics, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
authors: Ching-Feng
authors: CF
authors: Huang
authors: Chia-Chao
authors: CC
authors: Wu
authors: Der-Ming
authors: DM
authors: Chu
authors: Zo-Nan
authors: ZN
authors: Chang
authors: Fung-Lin
authors: FL
authors: Chue
authors: Ho-Jen
authors: HJ
authors: Peng
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D052179:Antigens, Plant / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D006109:Poaceae; D011058:Pollen / Q000009:adverse effects / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
chemical_list: D052179:Antigens, Plant; C550205:Cyn d 1 allergen, Bermuda grass; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma