title: Vimentin mediates uptake of C3 exoenzyme.
abstract: Clostridium botulinum C3 exoenzyme (C3) selectively inactivates RhoA/B/C GTPases by ADP-ribosylation. Based on this substrate specificity C3 is a well-established tool in cell biology. C3 is taken up by eukaryotic cells although lacking an uptake and translocation domain. Based on different approaches vimentin was identified as membranous C3-interaction partner by mass spectrometry. Vimentin in fact was partly localized at the outer surface of hippocampal HT22 cells and J744A.1 macrophages. Domain analysis identified the rod domain as binding partner of C3. Vimentin was also involved in uptake of C3 as shown by knock down of vimentin in HT22 and J774A.1 cells. The involvement of vimentin in uptake of C3 was further supported by the findings that the vimentin disruptor acrylamide blocked uptake of C3. Vimentin is not only a major organizing element of the intermediate filament network but is also involved in both binding and uptake of C3 exoenzyme.
authors: Institute of Toxicology, Hannover Medical School, Charité-Universitätsmedizin Berlin, Germany.
authors: Astrid
authors: A
authors: Rohrbeck
authors: Institute of Toxicology, Hannover Medical School, Charité-Universitätsmedizin Berlin, Germany.
authors: Anke
authors: A
authors: Schröder
authors: Institute of Toxicology, Hannover Medical School, Charité-Universitätsmedizin Berlin, Germany.
authors: Sandra
authors: S
authors: Hagemann
authors: Institute of Toxicology, Hannover Medical School, Charité-Universitätsmedizin Berlin, Germany.
authors: Andreas
authors: A
authors: Pich
authors: Center for Anatomy, Functional Cell Biology, Charité-Universitätsmedizin Berlin, Germany.
authors: Markus
authors: M
authors: Höltje
authors: Center for Anatomy, Functional Cell Biology, Charité-Universitätsmedizin Berlin, Germany.
authors: Gudrun
authors: G
authors: Ahnert-Hilger
authors: Institute of Toxicology, Hannover Medical School, Charité-Universitätsmedizin Berlin, Germany.
authors: Ingo
authors: I
authors: Just
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001905:Botulinum Toxins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D051379:Mice; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D011994:Recombinant Proteins; D014746:Vimentin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D014746:Vimentin; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins