title: Gamma-Irradiated Influenza A Virus Provides Adjuvant Activity to a Co-Administered Poorly Immunogenic SFV Vaccine in Mice.
abstract: Many currently available inactivated vaccines require "adjuvants" to maximize the protective immune responses generated against the antigens of interest. Recent studies in mice with gamma-irradiated influenza A virus (γ-FLU) have shown its superior efficacy compared to other forms of inactivated FLU vaccines and its ability to induce both potent interferon type-I (IFN-I) responses and the IFN-I-associated partial lymphocyte activation. Commonly, IFN-I responses induced by adjuvants, combined in vaccine preparations, have been shown to effectively enhance the immunogenicity of the antigens of interest. Therefore, we investigated the potential adjuvant activity of γ-FLU and the possible effect on antibody responses against co-administrated antigens, using gamma-irradiated Semliki Forest virus (γ-SFV) as the experimental vaccine in mice. Our data show that co-vaccination with γ-FLU and γ-SFV resulted in enhanced SFV-specific antibody responses in terms of increased titers by sixfold and greater neutralization efficacy, when compared to vaccination with γ-SFV alone. This study provides promising evidence related to the possible use of γ-FLU as an adjuvant to poorly immunogenic vaccines without compromising the vaccine efficacy of γ-FLU.
authors: Vaccine Research Laboratory, School of Molecular and Biomedical Science, Centre for Molecular Pathology, The University of Adelaide , Adelaide, SA , Australia.
authors: Rachelle
authors: R
authors: Babb
authors: Vaccine Research Laboratory, School of Molecular and Biomedical Science, Centre for Molecular Pathology, The University of Adelaide , Adelaide, SA , Australia.
authors: Jennifer
authors: J
authors: Chan
authors: Vaccine Research Laboratory, School of Molecular and Biomedical Science, Centre for Molecular Pathology, The University of Adelaide , Adelaide, SA , Australia.
authors: Jasmine E
authors: JE
authors: Khairat
authors: Department of Immunology, The John Curtin School of Medical Research, Australian National University , Canberra, ACT , Australia.
authors: Yoichi
authors: Y
authors: Furuya
authors: Vaccine Research Laboratory, School of Molecular and Biomedical Science, Centre for Molecular Pathology, The University of Adelaide , Adelaide, SA , Australia.
authors: Mohammed
authors: M
authors: Alsharifi
keywords: adjuvants; antibodies; combined vaccine; gamma-irradiation; influenza vaccines