title: Human β-defensin-3 inhibits migration of colon cancer cells via downregulation of metastasis-associated 1 family, member 2 expression.
abstract: The innate immune system plays an important role as the first line of defense against many types of microbes. Accumulating reports suggest that human β-defensins (hBDs) are expressed by and have certain roles in some cancer cells. In this study, we investigated the roles of hBD-3 in colon cancer cells. The expression of hBD-3 was examined by reverse transcriptase-polymerase chain reaction analysis of colon cancer cell lines and immunohistochemical staining of colon cancer tissues. The effect of hBD-3 on proliferation of colon cancer was assessed using the MTT assay and a real-time cell analyzer, and the effect of hBD-3 on the migration of colon cancer cells was also examined. The results showed that hBD-3 is not expressed in colon cancer cells but is produced by tumor-infiltrating monocytes. Migration of colon cancer cells was significantly inhibited by hBD-3 in a dose-dependent manner, although proliferation of colon cancer cells was not affected by administration of hBD-3. Moreover, reduced expression of metastasis-associated 1 family, member 2 (MTA2) mRNA in colon cancer cells was associated with exposure to hBD-3. In conclusion, progression of colon cancer was inhibited by hBD-3 in a paracrine fashion. Therefore, hBD-3 may be a potent new agent for treating colon cancer.
authors: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Satoko
authors: S
authors: Uraki
authors: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Kazushi
authors: K
authors: Sugimoto
authors: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Katsuya
authors: K
authors: Shiraki
authors: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Masahiko
authors: M
authors: Tameda
authors: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Yuji
authors: Y
authors: Inagaki
authors: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Suguru
authors: S
authors: Ogura
authors: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Chika
authors: C
authors: Kasai
authors: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Keiichiro
authors: K
authors: Nojiri
authors: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Misao
authors: M
authors: Yoneda
authors: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Norihiko
authors: N
authors: Yamamoto
authors: Department of Gastroenterology, Mie University School of Medicine, Tsu, Japan.
authors: Yoshiyuki
authors: Y
authors: Takei
authors: Department of Molecular and Laboratory Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Tsutomu
authors: T
authors: Nobori
authors: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
authors: Masaaki
authors: M
authors: Ito
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D019073:HT29 Cells; D006655:Histone Deacetylases / Q000235:genetics*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics*; D019899:Paracrine Communication; D012333:RNA, Messenger / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D023083:beta-Defensins / Q000235:genetics* / Q000378:metabolism*
chemical_list: C422950:DEFB103A protein, human; D012333:RNA, Messenger; D012097:Repressor Proteins; D023083:beta-Defensins; C120917:MTA2 protein, human; D006655:Histone Deacetylases