title: Quantitation of human parvovirus B19 DNA in erythema infectiosum and aplastic crisis.
abstract: Several publications concerning the methods of real-time PCR for human parvovirus B19 (B19V) have appeared and some case reports mention B19V DNA loads. However, no large-scale study quantitating levels of B19V DNA in common or representative B19V manifestations such as erythema infectiosum and aplastic crisis has been performed. Consequently, using the TaqMan PCR assay, the B19V load in a large sample of subjects with erythema infectiosum or aplastic crisis was quantitated. Sixty-five subjects in the acute phase of erythema infectiosum were involved, and in addition 22 serum samples from seven subjects with B19V-associated aplastic crisis complicating chronic hemolytic anemia were also analyzed. In the acute phase of erythema infectiosum the median B19V DNA load in the serum samples from the acute phase of erythema infectiosum was 7.63 × 10(5)  genomes/ml, (range from 4.48 × 10(3) to 8.31 × 10(6)  genomes/ml). The serum B19V DNA load during the acute phase of aplastic crisis complicating chronic hemolytic anemia was extremely high, that is 10(10) -10(13)  genomes/ml, and decreased gradually to around 10(5) genomes/ml over 1-2 months. Although all subjects followed an almost uniform and typical clinical course of erythema infectiosum, there was a large individual variation of B19V DNA loads, that is differences of over 1,000 times. Extremely high B19V loads were observed in subjects with aplastic crisis. This study is the first large scale report of studies of the B19V DNA loads in subjects with erythema infectiosum and aplastic crisis, the most common and significant clinical manifestations by B19V infections.
authors: Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Aki
authors: A
authors: Ishikawa
authors: Yuko
authors: Y
authors: Yoto
authors: Takeshi
authors: T
authors: Tsugawa
authors: Hiroyuki
authors: H
authors: Tsutsumi
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000097:blood*; D016731:Erythema Infectiosum / Q000150:complications / Q000821:virology*; D005260:Female; D006801:Humans; D008297:Male; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D044967:Serum / Q000821:virology; D019562:Viral Load; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: aplastic crisis; erythema infectiosum; hemolytic anemia; human parvovirus B19; real-time PCR