title: Molecular regulation of organic anion transporting polypeptide 1A2 (OATP1A2)by taurocholic acid in Bewo Cells.
abstract: To characterize the mechanisms of action of taurocholic acid(TCA) and farnesoid X receptor(FXR) on organic anion transporting polypeptide 1A2(OATP1A2) expression in placental Bewo cell line. Quantitative real-time PCR and Western blots were used to detect OATP1A2 in Bewo cells cultured with TCA and pcDNA3.1(+)-hFXR transfected Bewo cells after incubation with 2 mM TCA for 48 hours. TCA(0.02 mM) induced the mRNA and protein expression of OATP1A2 by 3 and 1.6 fold (p<0.05), respectively, while 0.2 and 2 mM TCA induced mRNA and protein expression by 1.5 and 1.3 fold, respectively. The concentration of TCA was negatively correlated with OATP1A2 gene expression (P<0.05). In pcDNA3.1(+)-hFXR transfected Bewo cells with 2 mM TCA demonstrated a 2-3 fold increase in OATP1A2 over controls (P<0.05). TCA is one of the regulation factors for OATP1A2 in the Bewo cell line. A low dose of TCA can induce fetal membrane expression of OATP1A2. This may present a physiological or compensatory mechanism of the placenta, while the high dose of TCA may produce a pathological or pathogenic mechanism. Farnesoid X receptor may act in synergy with TCA to increase the expression of OATP1A2. This may be a treatment strategy for fetal cholestasis.
authors: West China Women&apos;s & Children&apos;s Hospital, Sichuan University Department of gynecology & obstetrics Chengdu China.
authors: X H
authors: XH
authors: Yang
authors: West China Women&apos;s & Children&apos;s Hospital, Sichuan University Department of gynecology & obstetrics Chengdu China.
authors: S Y
authors: SY
authors: Liu
authors: West China Women&apos;s & Children&apos;s Hospital, Sichuan University Department of gynecology & obstetrics Chengdu China judyyang626&commat;gmail.com.
authors: A Y
authors: AY
authors: Xing
mesh_terms: D002460:Cell Line; D003902:Detergents / Q000737:chemistry / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D027361:Organic Anion Transporters / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D013656:Taurocholic Acid / Q000737:chemistry* / Q000494:pharmacology; D000099150:Receptor, Farnesoid X-Activated
chemical_list: D003902:Detergents; D027361:Organic Anion Transporters; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D013656:Taurocholic Acid; D000099150:Receptor, Farnesoid X-Activated; C512988:SLCO1A2 protein, human