title: Effect of FGF10 monoclonal antibody on psoriasis-like model in guinea pigs.
abstract: OBJECTIVE
  To investigate the therapeutical effect of topical application of FGF10 monoclonal antibody on the guinea pig model with psoriasis.
  
  
  METHODS
  Blank group, model group, hydrocortisone butyrate treatment group and high-dose (0.188 mg/ml), middle-dose (0.094 mg/ml) and low-dose (0.063 mg/ml) FGF10 antibody group were set, respectively. After two-week treatment, pathological changes of psoriasis-like models were observed by HE staining, and the difference in VEGF and PCNA expression levels among different groups was observed by immunohistochemical staining.
  
  
  RESULTS
  All the test indicators of each treatment group were lower than those of the model group, and there was a significant difference (P<0.05). The inflammatory cell count of the high-dose FGF10 antibody group was not statistically different from those of the blank group (t=0.77, P=0.443), and the counts of the rest treatment groups were significantly higher than those of the blank group and the high-dose FGF10 antibody group (P<0.05). The epidermal thickness of each FGF10 antibody treatment group was significantly higher than that of hydrocortisone butyrate treatment group (P<0.05), while no statistical difference was found in the epidermal thickness among the FGF10 antibody treatment groups (P>0.05). FGF10 monoclonal antibodies can reduce the PCNA and VEGF expression in psoriasis-like model of guinea pig's ear.
  
  
  CONCLUSIONS
  FGF10 monoclonal antibodies can affect keratinocyte proliferation and division and can also significantly inhibit the inflammatory response in the psoriasis model. Meanwhile, FGF10 monoclonal antibodies can produce a therapeutic effect on psoriatic lesions by inhibiting the abnormal epidermis cell proliferation and neovascularization of the dermis in the psoriasis model.
authors: Department of Dermatology, The Second Hospital of Jilin University Changchun 130041, China.
authors: Jian-Xin
authors: JX
authors: Xia
authors: Department of Dermatology, The Second Hospital of Jilin University Changchun 130041, China.
authors: Xiang-Lin
authors: XL
authors: Mei
authors: Department of Dermatology, The Second Hospital of Jilin University Changchun 130041, China.
authors: Wen-Jing
authors: WJ
authors: Zhu
authors: Department of Dermatology, The Second Hospital of Jilin University Changchun 130041, China.
authors: Xue
authors: X
authors: Li
authors: Department of Dermatology, The Second Hospital of Jilin University Changchun 130041, China.
authors: Xian-Hua
authors: XH
authors: Jin
authors: Department of Dermatology, The Second Hospital of Jilin University Changchun 130041, China.
authors: Yan
authors: Y
authors: Mou
authors: Department of Dermatology, The Second Hospital of Jilin University Changchun 130041, China.
authors: Kai
authors: K
authors: Yu
authors: Department of Dermatology, The Second Hospital of Jilin University Changchun 130041, China.
authors: Yi-Yu
authors: YY
authors: Wang
authors: Department of Dermatology, The Second Hospital of Jilin University Changchun 130041, China.
authors: Fu-Qiu
authors: FQ
authors: Li
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D003879:Dermatologic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D004817:Epidermis / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051526:Fibroblast Growth Factor 10 / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D006168:Guinea Pigs; D006854:Hydrocortisone / Q000031:analogs & derivatives / Q000494:pharmacology; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011565:Psoriasis / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D003879:Dermatologic Agents; D051526:Fibroblast Growth Factor 10; D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D006854:Hydrocortisone
keywords: FGF10; FGF10 monoclonal antibodies; PCNA; VEGF; psoriasis