title: Low-dose ethanol aggravates allergic dermatitis in mice.
abstract: Alcohol injures dendritic cells and suppresses cellular immunity, while some evidence indicates that drinking alcohol aggravates allergic asthma. This study investigated the effect of low doses of ethanol in enhancing allergic reactions in the skin of mice. Liquid food containing alcohol was administered to conventional NC/Nga mice to induce alcoholic hepatic steatosis, and spontaneous dermatitis was evaluated. BALB/c mice were administered approximately 1 g/kg body weight of ethanol by gavage, and contact hypersensitivity (CHS) or active cutaneous anaphylaxis (ACA) was induced. Spleens were collected 24 h after the elicitation of CHS and mRNA expressions of interferon (IFN)-γ, interleukin (IL)-4, IL-6, IL-10, and IL-18 were measured by quantitative RT-PCR. Alcohol-containing diet exaggerated spontaneous dermatitis in conventional NC/Nga mice and contact hypersensitivity in BALB/c mice. Ethanol administered by gavage for 5 days enhanced contact hypersensitivity in BALB/c mice. Ethanol administration with gavage also enhanced ACA of BALB/c mice. Ethanol did not affect mRNA expression of IFN-γ and IL-4, but did enhance IL-6, IL-10, and IL-18 mRNA expression. Histological evaluation revealed an absence of hepatic steatosis in mice administered ethanol by gavage for 5 days. In ethanol-administered mice, inflamed areas presented as lesions or a local extreme accumulation of mononuclear cells in the epidermis. These findings suggest that ethanol enhances the expression of inflammatory cytokines independently from T helper (Th)1/Th2 cytokine phenotypes, causing abnormalities in the epidermis resulting in exacerbated allergic reactivity.
authors: Laboratory of Toxicology, Faculty of Pharmacy, Osaka Ohtani University, 3-11-1 Nishikiori-kita, Tondabayashi, Osaka 584-8540, Japan. Electronic address: sakazato@osaka-ohtani.ac.jp.
authors: Fumitoshi
authors: F
authors: Sakazaki
authors: Department of Public Health & Preventive Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, 45-1 Nagaotoge-cho, Hirakata, Osaka 573-0101, Japan.
authors: Hirofumi
authors: H
authors: Ogino
authors: Department of Public Health & Preventive Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, 45-1 Nagaotoge-cho, Hirakata, Osaka 573-0101, Japan.
authors: Tomohiro
authors: T
authors: Arakawa
authors: Department of Public Health & Preventive Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, 45-1 Nagaotoge-cho, Hirakata, Osaka 573-0101, Japan.
authors: Tomofumi
authors: T
authors: Okuno
authors: Department of Public Health & Preventive Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, 45-1 Nagaotoge-cho, Hirakata, Osaka 573-0101, Japan.
authors: Hitoshi
authors: H
authors: Ueno
mesh_terms: D000707:Anaphylaxis / Q000276:immunology; D000818:Animals; D003876:Dermatitis, Atopic / Q000139:chemically induced*; D003877:Dermatitis, Contact / Q000209:etiology*; D004032:Diet; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D007371:Interferon-gamma; D016753:Interleukin-10; D015847:Interleukin-4; D015850:Interleukin-6; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology
chemical_list: D015850:Interleukin-6; D016753:Interleukin-10; D015847:Interleukin-4; D000431:Ethanol; D007371:Interferon-gamma
keywords: Alcohol; Allergy; Dermatitis; Ethanol