title: Protecting effect of caffeine against vinblastine (an anticancer drug) induced genotoxicity in mice.
abstract: Vinblastine a DNA non-intercalating agent has wide application against several human neoplasms, and found to cause cytogenotoxicity. In this study, clastogenotoxicity of vinblastine (1.5 mg/kg b w) and its prevention by caffeine at different doses (25, 50 and 100 mg/kg b w) administered intraperitoneally was assessed in in vivo mice. It was found that micronucleus level had decreased significantly (up to 28.8%) in 100 mg caffeine treated group at 30 h post treatment. However, it did not exhibit protective effect against chromosomal aberration in spaermatogonial cells at 24 h post treatment. The frequencies of aberrant primary spermatocytes had decreased significantly in 25 and 100 mg caffeine at 4th week of post treatment. Similarly, in 100 mg of caffeine administered, abnormal sperm level had reduced (4.01%) significantly at 8th week post treatment. Thus, caffeine decreased the vinblastine induced chromosomal aberrations and mitotic index in bone marrow cells. In conclusion, this study shows that caffeine exerts protective effect against vinblastin induced cytogenotoxicity. Further studies on molecular mechanism are interesting in order to develop it as an effective drug in cancer chemotherapy.
authors: Department of Applied Zoology, Kuvempu University , Shankaraghatta, Karnataka , India .
authors: Prakash
authors: P
authors: Geriyol
authors: Hosetti Basaling
authors: HB
authors: Basavanneppa
authors: Bhadrapura Lakkappa
authors: BL
authors: Dhananjaya
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000633:toxicity*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D002110:Caffeine / Q000008:administration & dosage / Q000494:pharmacology*; D002869:Chromosome Aberrations / Q000139:chemically induced; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D008940:Mitotic Index; D013090:Spermatocytes / Q000187:drug effects / Q000473:pathology; D014747:Vinblastine / Q000633:toxicity*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002110:Caffeine; D014747:Vinblastine
keywords: Antigenotoxicity; caffeine; chromosomal aberration; cytogenotoxicity; mice; mitotic index; sperm abnormality; vinblastine sulphate