title: Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells.
abstract: Fatty acid synthase (FAS) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. Sea buckthorn has long been used to treat a variety of maladies. Here, we investigated the inhibitory effect of sea buckthorn procyanidins (SBPs) isolated from the seeds of sea buckthorn on FAS and FAS overexpressed human breast cancer MDA-MB-231 cells. The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. We found that SBP potently inhibited the activity of FAS with a half-inhibitory concentration (IC50) value of 0.087 μg/ml. 3-4,5-Dimethylthiazol-2-yl-2,3-diphenyl tetrazolium bromide (MTT) assay was used to test the cell viability. SBP reduced MDA-MB-231 cell viability with an IC50 value of 37.5 μg/ml. Hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that SBP induced MDA-MB-231 cell apoptosis. SBP inhibited intracellular FAS activity with a dose-dependent manner. In addition, sodium palmitate could rescue the cell apoptosis induced by SBP. These results showed that SBP was a promising FAS inhibitor which could induce the apoptosis of MDA-MB-231 cells via inhibiting FAS. These findings suggested that SBP might be useful for preventing or treating breast cancer.
authors: College of Life Sciences, University of Chinese Academy of Sciences, No. 19A Yuquan Road, 100049, Beijing, China.
authors: Yi
authors: Y
authors: Wang
authors: Fangyuan
authors: F
authors: Nie
authors: Jian
authors: J
authors: Ouyang
authors: Xiaoyan
authors: X
authors: Wang
authors: Xiaofeng
authors: X
authors: Ma
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000201:enzymology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D064429:Fatty Acid Synthases / Q000037:antagonists & inhibitors* / Q000187:drug effects; D005434:Flow Cytometry; D031281:Hippophae / Q000737:chemistry; D006801:Humans; D008517:Phytotherapy / Q000379:methods*; D044945:Proanthocyanidins / Q000494:pharmacology*; D012639:Seeds
chemical_list: D044945:Proanthocyanidins; D064429:Fatty Acid Synthases