title: Metagenomic search strategies for interactions among plants and multiple microbes.
abstract: Plants harbor multiple microbes. Metagenomics can facilitate understanding of the significance, for the plant, of the microbes, and of the interactions among them. However, current approaches to metagenomic analysis of plants are computationally time consuming. Efforts to speed the discovery process include improvement of computational speed, condensing the sequencing reads into smaller datasets before BLAST searches, simplifying the target database of BLAST searches, and flipping the roles of metagenomic and reference datasets. The latter is exemplified by the e-probe diagnostic nucleic acid analysis approach originally devised for improving analysis during plant quarantine.
authors: Department of Biochemistry and Molecular Biology, Oklahoma State University Stillwater, OK, USA.
authors: Ulrich
authors: U
authors: Melcher
authors: Department of Biochemistry and Molecular Biology, Oklahoma State University Stillwater, OK, USA.
authors: Ruchi
authors: R
authors: Verma
authors: Foreign Disease-Weed Science Research Unit, United States Department of Agriculture - Agricultural Research Service Fort Detrick, MD, USA.
authors: William L
authors: WL
authors: Schneider
keywords: BLAST; EDNA; databases; e-probes; microbial consortia; taxonomic classification