title: Preclinical antitumor activity of the diindolylmethane formulation in xenograft mouse model of prostate cancer.
abstract: OBJECTIVE
  Preclinical study of the specific anticancer pharmacological activity of the formulation containing active substance 3,3ʹ-diindolylmethane (DIM), cod liver oil, polysorbate 80 and α-tocopherol acetate (vitamin E), in vivo in a xenograft animal model of LNCaP.
  
  
  METHODS
  The DIM, cod liver oil, polysorbate 80 and α-tocopherol acetate (vitamin E) formulation was intragastrically administered to BALB/c-nude (nu/nu) mice during 33 days post inoculation at the dose of 133 mg/kg/day. Antitumor activity of the test drug was estimated by the rate of tumor growth inhibition (T/C% - treated versus control), dividing the tumor volumes from treatment groups with the control groups.
  
  
  RESULTS
  Statistically significant tumor xenograft regressions have been shown in group which received the DIM, cod liver oil, polysorbate 80 and α-tocopherol acetate (vitamin E) on the 37(th) day of observation post inoculation. The highest antitumor activity was achieved on the 39(th) day (T/C = 16,8%). Therapeutic effect lasts for 6 days after the end of therapy period.
  
  
  CONCLUSIONS
  Our findings demonstrate inhibitory effect of the formulation on tumor development in the xenograft animal model due to the tumor growth rate reduction.
authors: Peoples' Friendship University of Russia, Moscow 117198, Russia.
authors: V I
authors: VI
authors: Kiselev
authors: Peoples' Friendship University of Russia, Moscow 117198, Russia.
authors: V M
authors: VM
authors: Drukh
authors: ZAO "MiraxBioPharma", Moscow 121248, Russia.
authors: E L
authors: EL
authors: Muyzhnek
authors: Moscow State Medical Stomatological University (MGMSU), Moscow 127473, Russia.
authors: I N
authors: IN
authors: Kuznetsov
authors: Peoples' Friendship University of Russia, Moscow 117198, Russia.
authors: O I
authors: OI
authors: Pchelintseva
authors: National Research Centre (NRC "Kurchatov Institute"), Moscow 123182, Russia.
authors: M A
authors: MA
authors: Paltsev
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D006801:Humans; D007211:Indoles / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D007211:Indoles; C016392:3,3'-diindolylmethane