title: Tityus serrulatus scorpion venom improves survival and lung inflammation in lethal sepsis induced by CLP in mice.
abstract: Tityus serrulatus venom (Tsv) modifies the behavior of immune cells and induces the production of inflammatory and anti-inflammatory cytokines; such action may interfere with physiological or pathological states. Because sepsis is characterized as an inflammatory disorder, the aim of present study was to investigate the effect of a non-lethal dose of Tsv in mice submitted to a polymicrobial infection by cecal ligation and puncture (CLP) model. The parameters evaluated were survival index, cellularity on lymphoid organs, peritoneal cavity and brochoalveolar space, production of IL-10, IL-12, IL-6, TNF-α, IFN-γ and MCP-1, pulmonary inflammation and oxidative burst. The results demonstrated that in sharp contrast to CLP group in which sepsis was lethal in a 24 h period all mice pretreated with Tsv survived even 60 h after CLP. Lung inflammation, another hallmark of CLP group, was also dramatically down regulated in Tsv/CLP group. Despite pretreatment with Tsv did not reduce the inflammatory serum cytokines when compared to CLP group; there was an increase in IL-10. In conclusion, subcutaneous Tsv administration 6 h before CLP was able to control the harmful effects of sepsis (lethality and lung inflammation). We suggest that both systemic IL-10 and oxidative burst are involved in this effect.
authors: Laboratório de Imunofisiologia, Departamento de Patologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal do Maranhão, São Luís, MA, Brazil.
authors: Márcia C G
authors: MC
authors: Maciel
authors: Laboratório de Imunofisiologia, Departamento de Patologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal do Maranhão, São Luís, MA, Brazil.
authors: Eder M S
authors: EM
authors: Fialho
authors: Laboratório de Imunofisiologia, Departamento de Patologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal do Maranhão, São Luís, MA, Brazil.
authors: Rosane N M
authors: RN
authors: Guerra
authors: FIOCRUZ, Centro de Pesquisas Gonçalo Moniz, Salvador, BA, Brazil.
authors: Valéria M
authors: VM
authors: Borges
authors: Laboratório de Imunofarmacologia, Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil.
authors: Fábio H
authors: FH
authors: Kwasniewski
authors: Laboratório de Imunofisiologia, Departamento de Patologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal do Maranhão, São Luís, MA, Brazil. Electronic address: nascimentofrf@yahoo.com.br.
authors: Flávia R F
authors: FR
authors: Nascimento
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood; D008297:Male; D051379:Mice; D011014:Pneumonia / Q000188:drug therapy*; D020011:Protective Agents / Q000627:therapeutic use*; D012604:Scorpion Venoms / Q000627:therapeutic use*; D018805:Sepsis / Q000188:drug therapy*
chemical_list: D016207:Cytokines; D020011:Protective Agents; D012604:Scorpion Venoms
keywords: CLP; Cytokines; Inflammation; Sepsis; Tityus serrulatus; Venom