title: Comparative Chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357).
abstract: Aspergillus oryzae and A. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. Bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. In this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of A. oryzae (RIB40) and A. flavus (NRRL 3357). Surprisingly, the overall chemical profiles of the two strains were mostly very different across 15 growth conditions. Contrary to previous studies we found the aflatrem precursor 13-desoxypaxilline to be a major metabolite from A. oryzae under certain growth conditions. For the first time, we additionally report A. oryzae to produce parasiticolide A and two new analogues hereof, along with four new alkaloids related to the A. flavus metabolites ditryptophenalines and miyakamides. Generally the secondary metabolite capability of A. oryzae presents several novel end products likely to result from the domestication process from A. flavus.
authors: Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Søltofts Plads B221, DK-2800 Kgs. Lyngby, Denmark. christian.rank@gmail.com.
authors: Christian
authors: C
authors: Rank
authors: Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Søltofts Plads B221, DK-2800 Kgs. Lyngby, Denmark. marlk@bio.dtu.dk.
authors: Marie Louise
authors: ML
authors: Klejnstrup
authors: Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Søltofts Plads B221, DK-2800 Kgs. Lyngby, Denmark. lmape@bio.dtu.dk.
authors: Lene Maj
authors: LM
authors: Petersen
authors: Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Søltofts Plads B221, DK-2800 Kgs. Lyngby, Denmark. sarakildgaard@hotmail.com.
authors: Sara
authors: S
authors: Kildgaard
authors: Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Søltofts Plads B221, DK-2800 Kgs. Lyngby, Denmark. jcf@bio.dtu.dk.
authors: Jens Christian
authors: JC
authors: Frisvad
authors: Department of Chemistry, Technical University of Denmark, Kemitorvet B201, DK-2800 Kgs. Lyngby, Denmark. chg@kemi.dtu.dk.
authors: Charlotte
authors: C
authors: Held Gotfredsen
authors: Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Søltofts Plads B221, DK-2800 Kgs. Lyngby, Denmark. tol@bio.dtu.dk.
authors: Thomas
authors: T
authors: Ostenfeld Larsen