title: Hepatoprotective effects of Mimic of Manganese superoxide dismutase against carbon tetrachloride-induced hepatic injury.
abstract: The aim of this study was to investigate the protective effects of Mimic of Manganese superoxide dismutase (MnSODm) against carbon tetrachloride (CCl(4))-induced hepatic injury in mice. Bifendate or MnSODm was intragastrically administered per day for 7 days. On the 8th day, all mice except the normal group were given 0.5% CCl(4)/peanut oil to induce hepatic injury model by intraperitoneal injection. Mice were sacrificed 24 h after CCl(4) treatment. Compared with the CCl(4) group, MnSODm significantly decreased the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the serum and increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the serum and liver. Moreover, the contents of hepatic and serum malondialdehyde (MDA) and nitric oxide (NO) with inducible nitric oxide synthase (iNOS) activities were reduced. Histological findings also confirmed the antihepatotoxic characterization. In addition, MnSODm inhibited the pro-inflammatory mediators, such as prostaglandin E(2) (PGE(2)), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Further investigation showed that the inhibitory effect of MnSODm on the pro-inflammatory cytokines was associated with the down-regulation of nuclear factor-kappa B (NF-κB). In brief, our results show that the protective effect of MnSODm against CCl(4)-induced hepatic injury may rely on its ability to reduce oxidative stress and suppress inflammatory responses.
authors: Department of Pharmacy, Gansu Provincial Hospital, Lanzhou 730000, Gansu, China.
authors: Yan-Hong
authors: YH
authors: Wang
authors: Division of Oncology, Gansu Provincial Hospital, Lanzhou 730000, Gansu, China.
authors: Xiang-Jiu
authors: XJ
authors: Xu
authors: Division of Oncology, Gansu Provincial Hospital, Lanzhou 730000, Gansu, China. Electronic address: lhl862010@126.com.
authors: Hong-Ling
authors: HL
authors: Li
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D040761:Biomimetic Materials / Q000008:administration & dosage* / Q000737:chemistry; D001713:Biphenyl Compounds / Q000008:administration & dosage*; D002251:Carbon Tetrachloride / Q000008:administration & dosage; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000276:immunology*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006576:Heterocyclic Compounds, 4 or More Rings / Q000008:administration & dosage / Q000737:chemistry; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001713:Biphenyl Compounds; D006576:Heterocyclic Compounds, 4 or More Rings; D018836:Inflammation Mediators; D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C465548:bifendate; D009569:Nitric Oxide; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Carbon tetrachloride; Mice; Mimic of Manganese superoxide dismutase; Nuclear factor-kappa B; Oxidative stress; Pro-inflammatory mediators