title: Antigenicity and conformational changes of β-lactoglobulin by dynamic high pressure microfluidization combining with glycation treatment.
abstract: The combined effect of previous dynamic high-pressure microfluidization treatment (40, 80, 120, and 160MPa) and subsequent glycation with galacto-oligosaccharides (GOS) on the antigenicity of β-lactoglobulin (β-LG) was investigated. The antigenicity of β-LG-GOS decreased at relatively low pressure (≤120MPa). Surface sulfhydryl group content of β-LG-GOS increased and surface hydrophobicity of β-LG-GOS decreased. Additionally, protein unfolding in β-LG-GOS samples was reflected by quenching of fluorescence intensity, the red-shift of fluorescence spectra, decreased UV absorption, and circular dichroism analysis, indicating tertiary and secondary structural changes of β-LG. The conformational changes may contribute to the alteration of antigenicity.
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: Junzhen
authors: J
authors: Zhong
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: Yue
authors: Y
authors: Tu
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: liuwei@ncu.edu.cn.
authors: Wei
authors: W
authors: Liu
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: Yujia
authors: Y
authors: Xu
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: Chengmei
authors: C
authors: Liu
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: Ruyan
authors: R
authors: Dun
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D005453:Fluorescence; D006031:Glycosylation; D057927:Hydrophobic and Hydrophilic Interactions; D007782:Lactoglobulins / Q000737:chemistry*; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry; D011312:Pressure; D011487:Protein Conformation
chemical_list: D007782:Lactoglobulins; D009844:Oligosaccharides
keywords: antigenicity; conformational change; dynamic high-pressure microfluidization combined with glycation; β-lactoglobulin