title: Cytokine effects on cell viability and death of prostate carcinoma cells.
abstract: We analyzed the effects of IL-13, IFN- γ , and IL-1 β on cell viability and death of LNCaP and PC-3 cells and major signaling pathways involved in these effects. Significant increase of LNCaP cell death (apoptotic and necrotic) and increased levels of active caspase 3 were observed in cells treated with inhibitors of ERK 1/2 (UO126) and p38 (SB203580) prior to IL-1 β treatment in comparison to cells treated with UO126, SB203580, or IL-1 β alone. Significant increase of LNCaP but not PC-3 cell death was detected after treatment with LY-294002 (inhibitor of phosphatidylinositol 3-kinase). No significant increase of LNCaP and PC-3 cell death was observed after treatment with SP600125 (inhibitor of JNK), SB203580 (inhibitor of p38), UO126 (inhibitor of ERK 1/2), or BAY 11-7082 (inhibitor of NF- κ B). Reduced c-FLIPL expression was observed in LNCaP cells treated with LY-294002. The significant potentiation of LNCaP cell death by inhibition of ERK 1/2, p38, and PI3-K pathways may provide a rationale for therapeutic approach in androgen-dependent prostate cancer.
authors: Department of Anatomy-Histology-Embryology, Medical School, University of Ioannina, 45110 Ioannina, Greece.
authors: Georgios
authors: 0000-0002-1645-2875
authors: G
authors: Chondrogiannis
authors: Department of Anatomy-Histology-Embryology, Medical School, University of Ioannina, 45110 Ioannina, Greece.
authors: Michalis
authors: 0000-0001-5296-9962
authors: M
authors: Kastamoulas
authors: Department of Anatomy-Histology-Embryology, Medical School, University of Ioannina, 45110 Ioannina, Greece.
authors: Panagiotis
authors: P
authors: Kanavaros
authors: Laboratory of Hematology, University Hospital of Ioannina, 45500 Ioannina, Greece.
authors: Georgios
authors: G
authors: Vartholomatos
authors: Department of Pathology, Medical School, University of Ioannina, 45110 Ioannina, Greece.
authors: Maria
authors: M
authors: Bai
authors: Department of Urology, Medical School, University of Ioannina, 45110 Ioannina, Greece.
authors: Dimitrios
authors: D
authors: Baltogiannis
authors: Department of Urology, Medical School, University of Ioannina, 45110 Ioannina, Greece.
authors: Nikolaos
authors: 0000-0003-1528-4029
authors: N
authors: Sofikitis
authors: Department of Anatomy, Medical School, University of Thessaly, 44110 Larisa, Greece.
authors: Dimitrios
authors: D
authors: Arvanitis
authors: Department of Anatomy-Histology-Embryology, Medical School, University of Ioannina, 45110 Ioannina, Greece.
authors: Vasiliki
authors: 0000-0002-6130-0061
authors: V
authors: Galani
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011419:Propidium / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000473:pathology*; D013194:Staining and Labeling; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D017304:Annexin A5; D016207:Cytokines; D016328:NF-kappa B; D011419:Propidium; D019869:Phosphatidylinositol 3-Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases