title: Enhancing effect of glucose microspheres in the viability of human mesenchymal stem cell suspensions for clinical administration.
abstract: OBJECTIVE
  A critical limiting factor of cell therapy is the short life of the stem cells. In this study, glucose containing alginate microspheres were developed and characterized to provide a sustained release system prolonging the viability of human mesenchymal stem cells (hMSCs) in a suspension for clinical application.
  
  
  METHODS
  The glucose microspheres were satisfactorily elaborated with alginate by emulsification/internal gelation method. Particle size was evaluated by light diffraction and optical microscopy. Shape and surface texture by scanning electron microscopy (SEM). Zeta potential, infrared spectra and release studies were also conducted. Also, rheological properties and stability of hMSCs suspensions with microspheres were tested. The viability of hMSCs was determined by trypan blue dye exclusion staining.
  
  
  RESULTS
  Microspheres of 86.62 μm, spherical shaped and -32.54 mV zeta potential with excellent stability, good encapsulation efficiency and providing an exponential release of glucose were obtained. hMSCs had better survival rate when they were packed with glucose microspheres. Microspheres maintained the aseptic conditions of the cell suspension without rheological, morphological or immunophenotypic disturbances on hMSCs.
  
  
  CONCLUSIONS
  Developed microspheres were able to enhance the functionality of hMSC suspension. This strategy could be broadly applied to various therapeutic approaches in which prolonged viability of cells is necessary.
authors: Andalusian Centre for Molecular Biology and Regenerative Medicine (CABIMER), Seville, Spain.
authors: Patricia
authors: P
authors: Gálvez
authors: Maria J
authors: MJ
authors: Martín
authors: Ana C
authors: AC
authors: Calpena
authors: Juan A
authors: JA
authors: Tamayo
authors: Maria A
authors: MA
authors: Ruiz
authors: Beatriz
authors: B
authors: Clares
mesh_terms: D000464:Alginates; D002470:Cell Survival / Q000187:drug effects; D004339:Drug Compounding; D004563:Electrochemistry; D004655:Emulsions; D005782:Gels; D005947:Glucose / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000276:immunology; D008863:Microspheres; D010316:Particle Size; D012212:Rheology; D013234:Stem Cells / Q000187:drug effects* / Q000276:immunology; D013242:Sterilization; D013535:Suspensions
chemical_list: D000464:Alginates; D004655:Emulsions; D005782:Gels; D013535:Suspensions; D005947:Glucose