title: In vivo monitoring of caspase activation using a fluorescence resonance energy transfer-based fluorescent probe.
abstract: Caspases, which constitute a family of cysteine proteases, are highly conserved in multicellular organisms and function as a central player in apoptosis. The detection of apoptosis is intrinsically difficult because dying cells are rapidly removed from tissues by phagocytosis. Thus, the development of a method for detecting caspase activation is critical for the in vivo study of apoptosis. In this chapter, we describe a genetically encoded fluorescent probe for live imaging of caspase activation.
authors: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan; PRESTO, JST, Tokyo, Japan. Electronic address: bunbun@mol.f.u-tokyo.ac.jp.
authors: Yoshifumi
authors: Y
authors: Yamaguchi
authors: Laboratory for Histogenetic Dynamics, RIKEN CDB, Kobe, Japan.
authors: Erina
authors: E
authors: Kuranaga
authors: Stowers Institute for Medical Research, Kansas, Missouri, USA.
authors: Yu-Ichiro
authors: Y
authors: Nakajima
authors: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
authors: Akiko
authors: A
authors: Koto
authors: PRESTO, JST, Tokyo, Japan; Department of Physiology, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
authors: Kiwamu
authors: K
authors: Takemoto
authors: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan; CREST, JST, Tokyo, Japan.
authors: Masayuki
authors: M
authors: Miura
mesh_terms: D000818:Animals; D020169:Caspases / Q000378:metabolism*; D004330:Drosophila / Q000201:enzymology / Q000254:growth & development; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods; D005260:Female; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis* / Q000378:metabolism; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D020169:Caspases
keywords: Apoptosis; FRET probe; caspase; drosophila; live-imaging; mouse