title: Water Soluble Components of 'Osteocare' Promote Cell Proliferation, Differentiation, and Matrix Mineralization in Human Osteoblast-Like SaOS-2 Cells.
abstract: Osteocare, a herbal formulation, has been found to be very effective in bone mineralization and support of the microstructure of bone tissue. The water-soluble components of Osteocare (WSCO) induced osteogenic activity in human osteoblast-like SaOS-2 cells. The addition of WSCO (100 μg/ml) to SaOS-2 cells was effective in increasing the cell proliferation by 41.49% and DNA content by 1.9-fold. WSCO increased matrix mineralization in SaOS-2 cells by increased alkaline phosphatase levels and calcium-rich deposits as observed by Alizarin red staining. WSCO markedly increased mRNA expression for osteopontin (OPN), osteocalcin (OCN), type I collagen (Col I) in SaOS-2 cells, and it down-regulated IL-6 mRNA levels in SaOS-2 cells. The present study showed that WSCO plays an important role in osteoblastic bone formation through enhanced activities of ALP, Col I, bone matrix proteins such as OPN and OCN, down-regulation of cytokines like IL-6, as well as promoting mineralization in SaOS-2 cells.
authors: Department of Cell Biology, Research and Development, The Himalaya Drug Company, Bangalore-562 162, India.
authors: Sandeep R
authors: SR
authors: Varma
authors: Department of Phytochemistry, Research and Development, The Himalaya Drug Company, Bangalore-562 162, India.
authors: L M
authors: LM
authors: Sharath Kumar
authors: Department of Cell Biology, Research and Development, The Himalaya Drug Company, Bangalore-562 162, India.
authors: Satyakumar
authors: S
authors: Vidyashankar
authors: Medical Services and Clinical Trials, Research and Development, The Himalaya Drug Company, Bangalore-562 162, India.
authors: Pralhad Sadashiv
authors: PS
authors: Patki
keywords: ALP activity; Cell proliferation; Col-I; IL-6; Mineralization; OCN; OPN; Osteocare