title: Subnormothermic machine perfusion for preservation of porcine kidneys in a donation after circulatory death model.
abstract: Machine perfusion for preservation led to compelling success for the outcome of renal transplantation. Further refinements of methods to decrease preservation injury remain an issue of high interest. This study investigates functional and morphological aspects of kidneys preserved by subnormothermic (20 °C) machine perfusion (SNTM) compared with oxygenated hypothermic machine perfusion (HMPox) and cold storage (CS) in a donation after circulatory death (DCD) model. After 30 min of warm ischaemia, porcine kidneys were randomly assigned to preservation for 7 h by CS, HMPox or SNTM. Afterwards, kidneys were reperfused for 2 h with autologous blood in vitro for assessment of function and integrity. Application of SNTM for preservation led to significantly higher blood flow and urine output compared with both other groups. SNTM led to a twofold increased creatinine clearance compared with HMPox and 10-fold increased creatinine clearance compared with CS. Structural integrity was best preserved by SNTM. In conclusion, this is the first study on SNTM for kidneys from DCD donors. SNTM seems to be a promising preservation method with the potential to improve functional parameters of kidneys during reperfusion.
authors: Department of General, Visceral and Transplantation Surgery, University Hospital Essen, Essen, Germany.
authors: Dieter P
authors: DP
authors: Hoyer
authors: Anja
authors: A
authors: Gallinat
authors: Sandra
authors: S
authors: Swoboda
authors: Jeremias
authors: J
authors: Wohlschläger
authors: Ursula
authors: U
authors: Rauen
authors: Andreas
authors: A
authors: Paul
authors: Thomas
authors: T
authors: Minor
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015925:Cryopreservation / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D007150:Immunohistochemistry; D007668:Kidney / Q000473:pathology* / Q000601:surgery; D016030:Kidney Transplantation / Q000379:methods*; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000379:methods; D011180:Postmortem Changes; D011897:Random Allocation; D012680:Sensitivity and Specificity; D012769:Shock; D013552:Swine
keywords: donation after circulatory death; kidney; machine perfusion; subnormothermia; temperature