title: Insertional hypermutation in mineral oil-induced plasmacytomas.
abstract: Unless stimulated by a chronic inflammatory agent, such as mineral oil, plasma cell tumors are rare in young BALB/c mice. This raises the questions: What do inflammatory tissues provide to promote mutagenesis? And what is the nature of mutagenesis? We determined that mineral oil-induced plasmacytomas produce large amounts of endogenous retroelements--ecotropic and polytropic murine leukemia virus and intracisternal A particles. Therefore, plasmacytoma formation might occur, in part, by de novo insertion of these retroelements, induced or helped by the inflammation. We recovered up to ten de novo insertions in a single plasmacytoma, mostly in genes with common retroviral integration sites. Additional integrations accompany tumor evolution from a solid tumor through several generations in cell culture. The high frequency of de novo integrations into cancer genes suggests that endogenous retroelements are coresponsible for plasmacytoma formation and progression in BALB/c mice.
authors: Department of Microbiology and Immunology, University of California, San Francisco, CA, USA.
authors: Gero
authors: G
authors: Knittel
authors: Mirjam
authors: M
authors: Metzner
authors: Gabriele
authors: G
authors: Beck-Engeser
authors: Ada
authors: A
authors: Kan
authors: Tomasz
authors: T
authors: Ahrends
authors: Dan
authors: D
authors: Eilat
authors: Konrad
authors: K
authors: Huppi
authors: Matthias
authors: M
authors: Wabl
mesh_terms: D000818:Animals; D002460:Cell Line; D004643:Emollients / Q000009:adverse effects* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008899:Mineral Oil / Q000009:adverse effects* / Q000494:pharmacology; D016254:Mutagenesis, Insertional / Q000187:drug effects / Q000276:immunology; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000473:pathology; D010954:Plasmacytoma / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000473:pathology; D018626:Retroelements
chemical_list: D004643:Emollients; D018626:Retroelements; D008899:Mineral Oil
keywords: Endogenous retroelements; Inflammation; Mutagenesis; Plasmacytomas; Tumor evolution