title: Development of multiplex PCR for rapid identification of four Salmonella serovars most commonly isolated in Japan.
abstract: More than 2,500 serovars of Salmonella species have been reported to date. A multiplex-PCR method was developed and evaluated for discriminating the four Salmonella enterica subsp enterica serovars, namely, S. Enteritidis, S. Typhimurium, S. Thompson and S. Infantis, most commonly isolated in Japan. Twenty-two serovars of 84 Salmonella strains and 7 species of non-Salmonella strains were evaluated using primer pairs specific for the detection of Salmonella spp. Multiplex PCR generated, with 100% specificity, the expected amplicon of 333, 413, 551 and 658 bp of S. Enteritidis, S. Infantis, S. Typhimurium, and S. Thompson, respectively, while an additional non-specific amplicon (about 1,000 bp) was observed for S. Infantis, but it had no practical impact in the bacterial detection. This multiplex PCR assay can be applied to identify and discriminate clinically significant strains of Salmonella serovars rapidly and accurately without the need for serological examination.
authors: Rika
authors: R
authors: Shimizu
authors: Kayo
authors: K
authors: Osawa
authors: Katsumi
authors: K
authors: Shigemura
authors: Hiroyuki
authors: H
authors: Yoshida
authors: Miki
authors: M
authors: Fujiwara
authors: Yoshio
authors: Y
authors: Iijima
authors: Masato
authors: M
authors: Fujisawa
authors: Toshiro
authors: T
authors: Shirakawa
mesh_terms: D004587:Electrophoresis, Agar Gel; D006801:Humans; D007564:Japan / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000235:genetics / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA