title: A static pressure sensitive receptor APJ promote H9c2 cardiomyocyte hypertrophy  via PI3K-autophagy pathway.
abstract: This study is designed to investigate whether APJ receptor acts as a sensor in static pressure-induced cardiomyocyte hypertrophy and to investigate the mechanism of PI3K-autophagy pathway. The left ventricular hypertrophy rat model was established by coarctation of abdominal aorta. H9c2 rat cardiomyocytes were cultured in the presence of static pressure which was given by a custom-made pressure incubator. The results revealed that the expression of apelin/APJ system, PI3K, Akt and their phosphorylation were significantly increased in the operation group. Static pressure up-regulated the APJ expression, PI3K phosphorylation, Akt phosphorylation, LC3-II/I and beclin-1 expression in cardiomyocytes. APJ shRNA pGPU6/Neo-rat-399, PI3K inhibitor LY294002, Akt inhibitor 1701-1 blocked the up-regulation of APJ, PI3K phosphorylation, Akt phosphorylation, LC3-II/I and beclin-1 expression, respectively. Moreover, static pressure increased the diameter, volume, protein content of cells, and these could be reversed when the cells were treated with pGPU6/Neo-rat-399, LY294002, and autophagy inhibitor 3-methyladenine, respectively. These results suggested that static pressure up-regulates APJ expression to promote cardiomyocyte hypertrophy by a PI3K-autophagy pathway.
authors: Feng
authors: F
authors: Xie
authors: Wei
authors: W
authors: Liu
authors: Fen
authors: F
authors: Feng
authors: Xin
authors: X
authors: Li
authors: Li
authors: L
authors: Yang
authors: Deguan
authors: D
authors: Lv
authors: Xuping
authors: X
authors: Qin
authors: Lanfang
authors: L
authors: Li
authors: Linxi
authors: L
authors: Chen
mesh_terms: D000818:Animals; D000075244:Apelin Receptors; D001343:Autophagy; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D032383:Myocytes, Cardiac / Q000201:enzymology / Q000473:pathology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000502:physiology*
chemical_list: D000075244:Apelin Receptors; C491284:Aplnr protein, rat; D017931:DNA Primers; D043562:Receptors, G-Protein-Coupled; D019869:Phosphatidylinositol 3-Kinases