title: Chlamydia muridarum infection associated host MicroRNAs in the murine genital tract and contribution to generation of host immune response.
abstract: OBJECTIVE
  Chlamydia trachomatis (CT) is the leading sexually transmitted bacterial infection in humans and is associated with reproductive tract damage. However, little is known about the involvement and regulation of microRNAs (miRs) in genital CT.
  
  
  METHODS
  We analyzed miRs in the genital tract (GT) following C. muridarum (murine strain of CT) challenge of wild type (WT) and CD4(+) T-cell deficient (CD4(-/-)) C57BL/6 mice at days 6 and 12 post-challenge.
  
  
  RESULTS
  At day 6, miRs significantly downregulated in the lower GT were miR-125b-5p, -16, -214, -23b, -135a, -182, -183, -30c, and -30e while -146 and -451 were significantly upregulated, profiles not exhibited at day 12 post-bacterial challenge. Significant differences in miR-125b-5p (+5.06-fold change), -135a (+4.9), -183 (+7.9), and -182 (+3.2) were observed in C. muridarum-infected CD4(-/-) compared to WT mice. In silico prediction and mass spectrometry revealed regulation of miR-135a and -182 and associated proteins, that is, heat-shock protein B1 and alpha-2HS-glycoprotein.
  
  
  CONCLUSIONS
  This study provides evidence on regulation of miRs following genital chlamydial infection suggesting a role in pathogenesis and host immunity.
authors: South Texas Center for Emerging Infectious Diseases and Center of Excellence in Infection Genomics, University of Texas at San Antonio, San Antonio, TX, USA.
authors: Rishein
authors: R
authors: Gupta
authors: Tanvi
authors: T
authors: Arkatkar
authors: Jieh-Juen
authors: JJ
authors: Yu
authors: Shradha
authors: S
authors: Wali
authors: William E
authors: WE
authors: Haskins
authors: James P
authors: JP
authors: Chambers
authors: Ashlesh K
authors: AK
authors: Murthy
authors: Sazaly Abu
authors: SA
authors: Bakar
authors: M Neal
authors: MN
authors: Guentzel
authors: Bernard P
authors: BP
authors: Arulanandam
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D058491:Bacterial Load; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002690:Chlamydia Infections / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D023502:Chlamydia muridarum; D010450:Endopeptidases / Q000276:immunology; D005260:Female; D005836:Genitalia, Female / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D006367:HeLa Cells; D006801:Humans; D007114:Immunization; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D035683:MicroRNAs; D010450:Endopeptidases; C425923:CPA factor
keywords: Chlamydia muridarum; host responses; microRNAs; murine genital tract