title: Effects of cigarette smoke extract on human airway smooth muscle cells in COPD.
abstract: We hypothesised that the response to cigarette smoke in airway smooth muscle (ASM) cells from smokers with chronic obstructive pulmonary disease (COPD) would be intrinsically different from smokers without COPD, producing greater pro-inflammatory mediators and factors relating to airway remodelling. ASM cells were obtained from smokers with or without COPD, and then stimulated with cigarette smoke extract (CSE) or transforming growth factor-β1. The production of chemokines and matrix metalloproteinases (MMPs) were measured by ELISA, and the deposition of collagens by extracellular matrix ELISA. The effects of CSE on cell attachment and wound healing were measured by toluidine blue attachment and cell tracker green wound healing assays. CSE increased the release of CXCL8 and CXCL1 from human ASM cells, and cells from smokers with COPD produced more CSE-induced CXCL1. The production of MMP-1, -3 and -10, and the deposition of collagen VIII alpha 1 (COL8A1) were increased by CSE, especially in the COPD group which had higher production of MMP-1 and deposition of COL8A1. CSE decreased ASM cell attachment and wound healing in the COPD group only. ASM cells from smokers with COPD were more sensitive to CSE stimulation, which may explain, in part, why some smokers develop COPD.
authors: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Shapingba, Chongqing, China Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia.
authors: Ling
authors: L
authors: Chen
authors: Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia Discipline of Pharmacology, The University of Sydney, Sydney, NSW, Australia.
authors: Qi
authors: Q
authors: Ge
authors: Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia Central Clinical School, The University of Sydney, Sydney, NSW, Australia.
authors: Gavin
authors: G
authors: Tjin
authors: Respiratory Research Group, Faculty of Pharmacy, The University of Sydney, Sydney, NSW, Australia.
authors: Hatem
authors: H
authors: Alkhouri
authors: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Shapingba, Chongqing, China Institute of Biomedical Engineering and Health Science, Changzhou University, Changzhou, Jiangsu, China.
authors: Linghong
authors: L
authors: Deng
authors: Dept of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
authors: Corry-Anke
authors: CA
authors: Brandsma
authors: Thoracic Medicine, Imperial College London, National Heart and Lung Institute, London, UK.
authors: Ian
authors: I
authors: Adcock
authors: Dept of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
authors: Wim
authors: W
authors: Timens
authors: Dept of Pulmonology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
authors: Dirkje
authors: D
authors: Postma
authors: Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia Discipline of Pharmacology, The University of Sydney, Sydney, NSW, Australia Central Clinical School, The University of Sydney, Sydney, NSW, Australia.
authors: Janette K
authors: JK
authors: Burgess
authors: Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia Discipline of Pharmacology, The University of Sydney, Sydney, NSW, Australia.
authors: Judith L
authors: JL
authors: Black
authors: Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia School of Medical and Molecular Biosciences, University of Technology Sydney, Sydney, NSW, Australia brian.oliver@uts.edu.au.
authors: Brian G G
authors: BG
authors: Oliver
mesh_terms: D056151:Airway Remodeling; D001980:Bronchi / Q000187:drug effects*; D002460:Cell Line; D002465:Cell Movement; D002470:Cell Survival; D024102:Collagen Type VIII / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000378:metabolism; D005452:Fluoresceins / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D029424:Pulmonary Disease, Chronic Obstructive / Q000209:etiology* / Q000503:physiopathology; D060888:Real-Time Polymerase Chain Reaction; D012137:Respiratory System / Q000187:drug effects; D012907:Smoking / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects; D014048:Tolonium Chloride / Q000737:chemistry; D018808:Transcription Factor AP-1 / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014945:Wound Healing
chemical_list: D024102:Collagen Type VIII; D005452:Fluoresceins; D018808:Transcription Factor AP-1; D053773:Transforming Growth Factor beta1; C069306:5-chloromethylfluorescein; D014048:Tolonium Chloride; D020782:Matrix Metalloproteinases; D020781:Matrix Metalloproteinase 1