title: Effect of wine processing and acute blood stasis on the serum pharmacochemistry of rhubarb: a possible explanation for processing mechanism.
abstract: As a specific item mentioned in traditional Chinese medicine theory, processing can fulfill different requirements of therapies. Crude and wine-processed rhubarbs are used as drastic and mild laxatives, respectively. In this study, a practical method based on ultra-fast liquid chromatography coupled with diode-array detection and ion trap time-of-flight mass spectrometry was developed to screen and analyze multiple absorbed bioactive components and metabolites in the serum of both normal and acute blood stasis rats after oral administration of crude or wine-processed rhubarbs. A total of 16 compounds, mainly including phase II metabolites, were tentatively identified. Possible explanations for the processing-induced changes in pharmacological effects of traditional Chinese medicines were first explored at serum pharmacochemistry level.
authors: Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Nanjing, China.
authors: Min
authors: M
authors: Wang
authors: Jinfeng
authors: J
authors: Fu
authors: Mengying
authors: M
authors: Lv
authors: Yuan
authors: Y
authors: Tian
authors: Fengguo
authors: F
authors: Xu
authors: Rui
authors: R
authors: Song
authors: Zunjian
authors: Z
authors: Zhang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001774:Blood Chemical Analysis; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis* / Q000494:pharmacology*; D008297:Male; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D051381:Rats; D017207:Rats, Sprague-Dawley; D012250:Rheum / Q000737:chemistry* / Q000378:metabolism*; D013997:Time Factors; D014920:Wine / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Herb processing; Mass spectrometry; Rhubarb; Serum pharmacochemistry