title: Three-dimensional tracking of Rab5- and Rab7-associated infection process of influenza virus.
abstract: Three-dimensional (3D) single-particle tracking (SPT) techniques have been widely reported. However, the 3D SPT technique remains poorly used for solving actual biological problems. In this work, a quantum dots (QDs)-based single-particle tracking technique is utilized to explore the Rab5- and Rab7-associated infection behaviors of influenza virus in three dimensions with a set of easily-attained equipment by the fast and accurate centroid method for 3D SPT. The experimental results indicate that Rab5 protein takes part in the virus infection process from the cell periphery to the perinuclear region, while Rab7 protein is mainly involved in the intermittent and confined movements of the virus in the perinuclear region. Evidently, the transition process of the virus-containing vesicles from early to late endosomes might occur during the intermittent movement in the perinuclear region. These findings reveal distinct dynamic behaviors of Rab5- and Rab7-positive endosomes in the course of the intracellular transport of viruses. This work is helpful in understanding the intracellular transport of cargoes.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology and Wuhan Institute of Biotechnology, Wuhan University, Wuhan, 430072, P. R. China.
authors: Shu-Lin
authors: SL
authors: Liu
authors: Qiu-Mei
authors: QM
authors: Wu
authors: Li-Juan
authors: LJ
authors: Zhang
authors: Zhi-Gang
authors: ZG
authors: Wang
authors: En-Ze
authors: EZ
authors: Sun
authors: Zhi-Ling
authors: ZL
authors: Zhang
authors: Dai-Wen
authors: DW
authors: Pang
mesh_terms: D000818:Animals; D004285:Dogs; D006801:Humans; D007251:Influenza, Human / Q000517:prevention & control*; D061985:Madin Darby Canine Kidney Cells; D020691:rab GTP-Binding Proteins / Q000502:physiology*; D020696:rab5 GTP-Binding Proteins / Q000502:physiology*; D000090802:rab7 GTP-Binding Proteins
chemical_list: D000090802:rab7 GTP-Binding Proteins; C000716463:rab7 GTP-binding proteins, human; D020691:rab GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins
keywords: Rab5; Rab7; influenza virus; quantum dots; three-dimensional single-particle tracking