title: Glutathione S-transferase A1 (GSTA1) release, an early indicator of acute hepatic injury in mice.
abstract: Three acute hepatic injury models (a CCl4-induced model, APAP-induced model and ethanol-induced model) in mice were used to study the importance of GSTA1 in acute hepatic injury by comparison with a standard enzyme marker, alanine aminotransferase (ALT). GSTA1 release was demonstrated to be an earlier and more sensitive indicator of hepatotoxicity than was ALT. Significant increases in GSTA1 were detected at 2 h after CCl4 exposure, while ALT was undetected at this time. GSTA1 was also a more sensitive indicator of hepatotoxicity than ALT after 6 h. In the APAP and ethanol models, GSTA1 was markedly increased earlier than ALT, at 2 h post exposure. The release of GSTA1 was significantly increased at a dose of 12.5 mg/kg (CCl4 model), 100 mg/kg (APAP model) and 10 ml/kg (ethanol model), the lowest exposure concentration for each model. In contrast, AST release was not statistically significant. These results suggest that GSTA1 can be detected at low concentrations during the early stages of acute hepatic injury and that GSTA1 is a more sensitive and more accurate indicator than ALT.
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China. Electronic address: dongnongyaoli@126.com.
authors: Fangping
authors: F
authors: Liu
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors: Yuexia
authors: Y
authors: Lin
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors: Zhi
authors: Z
authors: Li
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors: Xin
authors: X
authors: Ma
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors: Qing
authors: Q
authors: Han
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors: Yingshu
authors: Y
authors: Liu
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors: Qiong
authors: Q
authors: Zhou
authors: Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, PR China.
authors: Jingli
authors: J
authors: Liu
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors: Rui
authors: R
authors: Li
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors: Jichang
authors: J
authors: Li
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors: Li
authors: L
authors: Gao
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology*; D005982:Glutathione Transferase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D051379:Mice
chemical_list: D007527:Isoenzymes; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha
keywords: Acute hepatic injury; Dose–response; GSTA1; Indicator; Time–response