title: Functional divergence in gastrointestinal microbiota in physically-separated genetically identical mice.
abstract: Despite the fundamental contribution of the gut microbiota to host physiology, the extent of its variation in genetically-identical animals used in research is not known. We report significant divergence in both the composition and metabolism of gut microbiota in genetically-identical adult C57BL/6 mice housed in separate controlled units within a single commercial production facility. The reported divergence in gut microbiota has the potential to confound experimental studies using mammalian models.
authors: 1] Immunity, Infection, and Inflammation Program, Mater Research Institute - University of Queensland, Translational Research Institute, Woolloongabba, Australia [2] King's College London, Institute of Pharmaceutical Science, London, SE1 9NH, UK [3] SAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Bedford Park, Adelaide, Australia.
authors: G B
authors: GB
authors: Rogers
authors: King's College London, Institute of Pharmaceutical Science, London, SE1 9NH, UK.
authors: J
authors: J
authors: Kozlowska
authors: King's College London, Institute of Pharmaceutical Science, London, SE1 9NH, UK.
authors: J
authors: J
authors: Keeble
authors: Charles River UK, Manston Rd. Margate, Kent CT9 4LT UK.
authors: K
authors: K
authors: Metcalfe
authors: Charles River UK, Manston Rd. Margate, Kent CT9 4LT UK.
authors: M
authors: M
authors: Fao
authors: Molecular Research MR DNA, Shallowater, TX 79363, USA.
authors: S E
authors: SE
authors: Dowd
authors: King's College London, Institute of Pharmaceutical Science, London, SE1 9NH, UK.
authors: A J
authors: AJ
authors: Mason
authors: Immunity, Infection, and Inflammation Program, Mater Research Institute - University of Queensland, Translational Research Institute, Woolloongabba, Australia.
authors: M A
authors: MA
authors: McGuckin
authors: King's College London, Institute of Pharmaceutical Science, London, SE1 9NH, UK.
authors: K D
authors: KD
authors: Bruce
mesh_terms: D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development; D044822:Biodiversity; D016000:Cluster Analysis; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology*; D014644:Genetic Variation; D055442:Metabolome; D055432:Metabolomics / Q000145:classification / Q000379:methods; D008810:Mice, Inbred C57BL; D064307:Microbiota / Q000235:genetics*; D066244:Proton Magnetic Resonance Spectroscopy; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012934:Social Isolation
chemical_list: D012336:RNA, Ribosomal, 16S