title: Enhanced enrichment of prostate cancer stem-like cells with miniaturized 3D culture in liquid core-hydrogel shell microcapsules.
abstract: Cancer stem-like cells (CSCs) are rare subpopulations of cancer cells that are reported to be responsible for cancer resistance and metastasis associated with conventional cancer therapies. Therefore, effective enrichment/culture of CSCs is of importance to both the understanding and treatment of cancer. However, it usually takes approximately 10 days for the widely used conventional approach to enrich CSCs through the formation of CSC-containing aggregates. Here we report the time can be shortened to 2 days while obtaining prostate CSC-containing aggregates with better quality based on the expression of surface receptor markers, dye exclusion, gene and protein expression, and in vivo tumorigenicity. This is achieved by encapsulating and culturing human prostate cancer cells in the miniaturized 3D liquid core of microcapsules with an alginate hydrogel shell. The miniaturized 3D culture in core-shell microcapsules is an effective strategy for enriching/culturing CSCs in vitro to facilitate cancer research and therapy development.
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA.|Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
authors: Wei
authors: W
authors: Rao
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA.|Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
authors: Shuting
authors: S
authors: Zhao
authors: Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.|Division of Hematology, The Ohio State University, Columbus, OH 43210, USA.
authors: Jianhua
authors: J
authors: Yu
authors: Department of Cancer Biology, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
authors: Xiongbin
authors: X
authors: Lu
authors: Division of Genitourinary Pathology, The Ohio State University, Columbus, OH 43210, USA.
authors: Debra L
authors: DL
authors: Zynger
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA.|Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.|Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
authors: Xiaoming
authors: X
authors: He
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002214:Capsules; D063646:Carcinogenesis / Q000187:drug effects / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D007150:Immunohistochemistry; D007700:Kinetics; D008297:Male; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology*; D010455:Peptides / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D058985:Side-Population Cells / Q000166:cytology / Q000187:drug effects; D018874:Spheroids, Cellular / Q000187:drug effects / Q000473:pathology
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D002214:Capsules; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D010455:Peptides; D011956:Receptors, Cell Surface; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: Alginate; Cancer stem-like cells; Hydrogel; Microcapsule; Miniaturized 3D culture