title: Intelligent layered nanoflare: "lab-on-a-nanoparticle" for multiple DNA logic gate operations and efficient intracellular delivery.
abstract: DNA strand displacement cascades have been engineered to construct various fascinating DNA circuits. However, biological applications are limited by the insufficient cellular internalization of naked DNA structures, as well as the separated multicomponent feature. In this work, these problems are addressed by the development of a novel DNA nanodevice, termed intelligent layered nanoflare, which integrates DNA computing at the nanoscale, via the self-assembly of DNA flares on a single gold nanoparticle. As a "lab-on-a-nanoparticle", the intelligent layered nanoflare could be engineered to perform a variety of Boolean logic gate operations, including three basic logic gates, one three-input AND gate, and two complex logic operations, in a digital non-leaky way. In addition, the layered nanoflare can serve as a programmable strategy to sequentially tune the size of nanoparticles, as well as a new fingerprint spectrum technique for intelligent multiplex biosensing. More importantly, the nanoflare developed here can also act as a single entity for intracellular DNA logic gate delivery, without the need of commercial transfection agents or other auxiliary carriers. By incorporating DNA circuits on nanoparticles, the presented layered nanoflare will broaden the applications of DNA circuits in biological systems, and facilitate the development of DNA nanotechnology.
authors: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, P.R. China. xbzhang@hnu.cn tan@chem.ufl.edu.
authors: Bin
authors: B
authors: Yang
authors: Xiao-Bing
authors: XB
authors: Zhang
authors: Li-Ping
authors: LP
authors: Kang
authors: Zhi-Mei
authors: ZM
authors: Huang
authors: Guo-Li
authors: GL
authors: Shen
authors: Ru-Qin
authors: RQ
authors: Yu
authors: Weihong
authors: W
authors: Tan
mesh_terms: D000465:Algorithms; D015374:Biosensing Techniques; D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D053761:Nanocomposites / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*
chemical_list: D006046:Gold; D004247:DNA