title: Evolutionarily conserved transcription factor Apontic controls the G1/S progression by inducing cyclin E during eye development.
abstract: During Drosophila eye development, differentiation initiates in the posterior region of the eye disk and progresses anteriorly as a wave marked by the morphogenetic furrow (MF), which demarcates the boundary between anterior undifferentiated cells and posterior differentiated photoreceptors. However, the mechanism underlying the regulation of gene expression immediately before the onset of differentiation remains unclear. Here, we show that Apontic (Apt), which is an evolutionarily conserved transcription factor, is expressed in the differentiating cells posterior to the MF. Moreover, it directly induces the expression of cyclin E and is also required for the G1-to-S phase transition, which is known to be essential for the initiation of cell differentiation at the MF. These observations identify a pathway crucial for eye development, governed by a mechanism in which Cyclin E promotes the G1-to-S phase transition when regulated by Apt.
authors: Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China;Center for Information Biology and liuqingxin@sdau.edu.cn takashi.gojobori@kaust.edu.sa.
authors: Qing-Xin
authors: QX
authors: Liu
authors: Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China;
authors: Xian-Feng
authors: XF
authors: Wang
authors: Center for Information Biology and.
authors: Kazuho
authors: K
authors: Ikeo
authors: Department of Developmental Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan;
authors: Susumu
authors: S
authors: Hirose
authors: Department of Cell Biology, Biozentrum, University of Basel, 4056 Basel, Switzerland; liuqingxin@sdau.edu.cn takashi.gojobori@kaust.edu.sa.
authors: Walter J
authors: WJ
authors: Gehring
authors: Center for Information Biology andComputational Bioscience Research Center, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia; andDepartment of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan, Republic of China liuqingxin@sdau.edu.cn takashi.gojobori@kaust.edu.sa.
authors: Takashi
authors: T
authors: Gojobori
mesh_terms: D000818:Animals; D019927:Cyclin E / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000502:physiology; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins / Q000378:metabolism* / Q000502:physiology; D024202:Electrophoretic Mobility Shift Assay; D005123:Eye / Q000196:embryology*; D059585:G1 Phase Cell Cycle Checkpoints / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D009841:Oligonucleotides / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000378:metabolism* / Q000502:physiology
chemical_list: D019927:Cyclin E; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009841:Oligonucleotides; D014157:Transcription Factors; C108529:apt protein, Drosophila