title: Retinol status and expression of retinol-related proteins in methionine-choline deficient rats.
abstract: Retinol and its derivative, retinoic acid, have pleiotropic functions including vision, immunity, hematopoiesis, reproduction, cell differentiation/growth, and development. Non-alcoholic fatty liver disease (NAFLD) is one of the most common diseases in developed countries and encompasses a broad spectrum of forms, ranging from steatosis to steatohepatitis, which develops further to cirrhosis. Retinol status has an important role in liver homeostasis. The purpose of this study was to evaluate the retinol status and expression of retinol-related proteins, including enzymes and binding proteins, in methionine-choline deficient (MCD) rats as a model of NAFLD. We examined retinol levels in the plasma and liver and gene expression for β-carotene 15,15'-monooxygenase (BCMO), lecithIn: retinol acyltransferase (LRAT), aldehyde dehydrogenase 1A1 (ALDH1A1), ALDH1A2, and cellular retinol binding protein (CRBP)-I in MCD rats. The plasma retinol levels in MCD rats were lower than those in the controls, whereas hepatic retinol levels in MCD rats were higher. BCMO expression in the intestine and liver in MCD rats was lower, whereas that in the testes and the kidneys was higher than in control rats. Expression of LRAT, CRBP-I, ALDH1A1, and ALDH1A2 in the liver of MCD rats was also higher. Altered expression of retinol-related proteins may affect retinol status in NAFLD.
authors: Department of Pediatrics, Osaka Medical College.
authors: Hiroshi
authors: H
authors: Miyazaki
authors: Kimitaka
authors: K
authors: Takitani
authors: Maki
authors: M
authors: Koh
authors: Akiko
authors: A
authors: Inoue
authors: Kanta
authors: K
authors: Kishi
authors: Hiroshi
authors: H
authors: Tamai
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000818:Animals; D002796:Choline Deficiency / Q000150:complications; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000172:deficiency; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology / Q000378:metabolism*; D017208:Rats, Wistar; D054840:Retinol-Binding Proteins, Cellular / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D014801:Vitamin A / Q000097:blood / Q000378:metabolism*; D050559:beta-Carotene 15,15'-Monooxygenase / Q000378:metabolism*
chemical_list: D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A; D008715:Methionine; D050559:beta-Carotene 15,15'-Monooxygenase; D000444:Aldehyde Dehydrogenase; D000217:Acyltransferases; C056942:lecithin-retinol acyltransferase