title: CD8+HLA-DR+ T cells are increased in patients with severe aplastic anemia.
abstract: The aim of the present study was to investigate the number and function of CD8+HLA-DR+ cells, which are considered to be activated cytotoxic T lymphocytes (CTLs), in peripheral blood to further examine the pathogenesis of severe aplastic anemia (SAA). Thirty-eight patients with SAA were included in the present study. Patients were screened for paroxysmal nocturnal hemoglobinuria by flow cytometry using anti-CD55 and anti-CD59 antibodies. The number of CD8+HLA-DR+ T cells was measured by three-color flow cytometry using anti-CD8-peridinin chlorophyll, anti-CD3-fluorescein isothiocyanate (FITC) and anti-HLA-DR-FITC antibodies. The expression of perforin, granzyme B, tumor necrosis factor-β (TNF-β) and FasL in CD8+HLA-DR+ T cells was detected by flow cytometry with the appropriate monoclonal antibodies. Total RNA was prepared from purified CD8+HLA-DR+ cells of healthy controls and SAA patients, and then polymerase chain reaction (PCR) was performed. Apoptosis of CD8+HLA-DR+ cells was detected by flow cytometry following staining with Annexin V. The proportion of CD8+HLA-DR+ T cells was analyzed by flow cytometry in peripheral blood and was identified to be significantly higher in untreated SAA than in remission patients and in the controls. The expression of perforin, granzyme B, TNF-β and FasL in CD8+HLA-DR+ T cells was analyzed by flow cytometry and PCR, which revealed increased expression in the untreated SAA group compared with that in the control group. Furthermore, the apoptosis of CD3- bone marrow cells from normal individuals was enhanced following co-culture with CD8+HLA-DR+ T cells from untreated SAA patients. In conclusion, the present study demonstrated that CD8+HLA-DR+ T cells may contribute to bone marrow failure in SAA.
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Limin
authors: L
authors: Xing
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Chunyan
authors: C
authors: Liu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Rong
authors: R
authors: Fu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Huaquan
authors: H
authors: Wang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Jun
authors: J
authors: Wang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Xiao
authors: X
authors: Liu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Le
authors: L
authors: Feng
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Lijuan
authors: L
authors: Li
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Hui
authors: H
authors: Liu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Honglei
authors: H
authors: Wang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Tian
authors: T
authors: Zhang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors: Zonghong
authors: Z
authors: Shao
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000276:immunology* / Q000473:pathology; D017209:Apoptosis / Q000502:physiology; D001854:Bone Marrow Cells / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D016022:Case-Control Studies; D002648:Child; D018920:Coculture Techniques; D053222:Fas Ligand Protein / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D053804:Granzymes / Q000235:genetics / Q000378:metabolism; D006684:HLA-DR Antigens / Q000378:metabolism*; D064368:Healthy Volunteers; D006801:Humans; D008233:Lymphotoxin-alpha / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D054353:Perforin / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D055815:Young Adult
chemical_list: C505661:FASLG protein, human; D053222:Fas Ligand Protein; D006684:HLA-DR Antigens; D008233:Lymphotoxin-alpha; D012333:RNA, Messenger; D054353:Perforin; D053804:Granzymes