title: Enhancement of enzymatic saccharification of Eucalyptus globulus: steam explosion versus steam treatment.
abstract: Steam explosion and steam pre-treatment have proved capable of enhancing enzymatic saccharification of lignocellulosic materials. However, until now, these methods had not been compared under the same operational conditions and using the same raw material. Both pre-treatments lead to increased yields in the saccharification of Eucalyptus globulus; but results have been better with steam pre-treatments, despite the more accessible surface of exploded samples. The reason for this finding could be enzymatic inhibition: steam explosion causes a more extensive extraction of hemicelluloses and releases a greater amount of degradation products which can inhibit enzymatic action. Enzymatic inhibition is also dependent on the amount and chemical structure of lignin, which was also a contributing factor to the lower enzymatic yields obtained with the most severe pre-treatment. Thus, the highest yields (46.7% glucose and 73.4% xylose yields) were obtained after two cycle of steam treatment, of 5 and 3 min, at 183°C.
authors: INIA-CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra de la Coruña Km 7.5, Madrid 28040, Spain. Electronic address: martin.raquel@inia.es.
authors: Raquel
authors: R
authors: Martin-Sampedro
authors: INIA-CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra de la Coruña Km 7.5, Madrid 28040, Spain.
authors: Esteban
authors: E
authors: Revilla
authors: INIA-CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra de la Coruña Km 7.5, Madrid 28040, Spain.
authors: Juan C
authors: JC
authors: Villar
authors: INIA-CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra de la Coruña Km 7.5, Madrid 28040, Spain.
authors: Maria E
authors: ME
authors: Eugenio
mesh_terms: D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000378:metabolism*; D005052:Eucalyptus / Q000378:metabolism*; D005947:Glucose / Q000032:analysis; D008031:Lignin / Q000302:isolation & purification; D013227:Steam; D014990:Xylans / Q000302:isolation & purification; D014994:Xylose / Q000032:analysis
chemical_list: D013227:Steam; D014990:Xylans; D008031:Lignin; D014994:Xylose; D002480:Cellulase; D005947:Glucose
keywords: Autohydrolysis; Enzymatic saccharification; Eucalyptus globulus; Steam explosion