title: Engineering interlocking DNA rings with weak physical interactions.
abstract: Catenanes are intriguing molecular assemblies for engineering unique molecular devices. The resident rings of a catenane are expected to execute unhindered rotation around each other, and to do so, they must have weak physical interactions with each other. Due to sequence programmability, DNA has become a popular material for nanoscale object engineering. However, current DNA catenanes, particularly in the single-stranded (ss) form, are synthesized through the formation of a linking duplex, which makes them less ideal as mobile elements for molecular machines. Herein we adopt a random library approach to engineer ssDNA [2] catenanes (two interlocked DNA rings) without a linking duplex. Results from DNA hybridization, double-stranded catenane synthesis and rolling circle amplification experiments signify that representative catenanes have weak physical interactions and are capable of operating as independent units. Our findings lay the foundation for exploring free-functioning interlocked DNA rings for the design of elaborate nanoscale machines based on DNA.
authors: 1] Department of Biochemistry and Biomedical Sciences, Michael G. DeGroote Institute of Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1 [2] Department of Chemistry and Chemical Biology, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1 [3] Cancer Metastasis Alert and Prevention Center, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China [4].
authors: Zai-Sheng
authors: ZS
authors: Wu
authors: 1] Department of Biochemistry and Biomedical Sciences, Michael G. DeGroote Institute of Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1 [2] Department of Chemistry and Chemical Biology, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1 [3].
authors: Zhifa
authors: Z
authors: Shen
authors: 1] Department of Biochemistry and Biomedical Sciences, Michael G. DeGroote Institute of Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1 [2] Department of Chemistry and Chemical Biology, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1.
authors: Kha
authors: K
authors: Tram
authors: 1] Department of Biochemistry and Biomedical Sciences, Michael G. DeGroote Institute of Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1 [2] Department of Chemistry and Chemical Biology, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1.
authors: Yingfu
authors: Y
authors: Li
mesh_terms: D043864:DNA, Catenated / Q000138:chemical synthesis* / Q000737:chemistry; D004277:DNA, Single-Stranded; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D043864:DNA, Catenated; D004277:DNA, Single-Stranded