title: Rapid aptasensor capable of simply diagnosing prostate cancer.
abstract: Using guanine (G)-rich DNA aptamer-conjugated 6-carboxyfluorescein (6-FAM) capable of rapidly capturing prostate specific antigen (PSA) in human serum, cost-effective and simple biosensor with guanine chemiluminescence detection was developed for early diagnosis of prostate cancer. Free G-rich DNA aptamer-conjugated 6-FAM emits bright light in guanine chemiluminescence reaction based on the principle of chemiluminescent resonance energy transfer (CRET). However, G-rich DNA aptamer-conjugated 6-FAM bound with PSA cannot emit light because PSA acts as a strong interference in CRET between 6-FAM and high-energy intermediate formed from the reaction of 3,4,5-trimethoxylphenylglyoxal (TMPG) and guanine of G-rich DNA aptamer. A chemiluminescent biosensor, developed using the different properties of G-rich DNA aptamer-conjugated 6-FAM in the absence and presence of PSA in guanine chemiluminescence reaction, was able to quantify trace levels of PSA in human serum within 30 min without time-consuming and complicated procedures (e.g., multiple incubation and washings) required for conventional immunoassays operated with expensive and intractable antibodies. The limit of detection of chemiluminescent biosensor having a wide linear dynamic range (1.9-125 ng/ml) was 1.0 ng/ml. The excellent correlation (R=0.985) between chemiluminescent biosensor and conventional enzyme immunoassay indicates that the accurate, precise, and rapid chemiluminescent biosensor can be applied as a new method for early diagnosis of prostate cancer.
authors: Luminescent MD, LLC, Hagerstown, MD 21742, United States; Thomas Jefferson High School for Science and Technology, Alexandria, VA 22312, United States.
authors: Timothy
authors: T
authors: Cha
authors: Luminescent MD, LLC, Hagerstown, MD 21742, United States; McLean High School, McLean, VA 22101, United States.
authors: Sandy
authors: S
authors: Cho
authors: Department of Wood Science & Forest Products, Virginia Polytechnic Institute & State University, Blacksburg, VA 24061, United States.
authors: Young Teck
authors: YT
authors: Kim
authors: Luminescent MD, LLC, Hagerstown, MD 21742, United States. Electronic address: jhlee@luminescentmd.com.
authors: Ji Hoon
authors: JH
authors: Lee
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D004735:Energy Transfer; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000191:economics / Q000379:methods; D008297:Male; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis
chemical_list: D052157:Aptamers, Nucleotide; D005452:Fluoresceins; D005456:Fluorescent Dyes; C024098:6-carboxyfluorescein; D006147:Guanine; D017430:Prostate-Specific Antigen
keywords: 3,4,5-trimethoxylphenylglyoxal (TMPG); Chemiluminescent resonance energy transfer (CRET); Diagnosis; G-rich DNA aptamer; Guanine chemiluminescence; Prostate specific antigen (PSA)