title: Phase separation in mixtures of ovalbumin and konjac glucomannan: physicochemical and microscopic investigations.
abstract: The phase behavior and microstructure of ovalbumin (OVA)/konjac glucomannan (KGM) mixtures were studied at pH 7.0. Phase diagrams were established by centrifugation and visual observation. Micro-phase separation of the OVA/KGM mixtures was quantified by measuring the turbidity. The microstructures of the phase separated mixtures were studied by measuring rheological property and confocal laser scanning microscopy (CLSM). The phase behavior of OVA/KGM mixtures appeared to be one single phase or two separated phases depending on the content of OVA and KGM. OVA had a pronounced effect on turbidity of OVA/KGM mixtures. The particle size of mixtures increased with increasing OVA and KGM concentration, which was the largest (119.1 μm) at 0.25 wt.% KGM and 5 wt.% OVA. The G' and G″ cross-over at a mixture of 0.20 wt.% KGM and 4 wt.% OVA demonstrated the buildup of microstructure during phase separation. The association of OVA aggregates could be observed under CLSM.
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, China.
authors: Bin
authors: B
authors: Zhou
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, China.
authors: Jinjin
authors: J
authors: Liu
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, China.
authors: Ting
authors: T
authors: Ye
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, China.
authors: Yuntao
authors: Y
authors: Wang
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, China.
authors: Wei
authors: W
authors: Xu
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, China. Electronic address: libinfood@mail.hzau.edu.cn.
authors: Bin
authors: B
authors: Li
mesh_terms: D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D010047:Ovalbumin / Q000737:chemistry* / Q000302:isolation & purification; D010316:Particle Size; D012212:Rheology; D014783:Viscosity
chemical_list: D008351:Mannans; C022901:(1-6)-alpha-glucomannan; D010047:Ovalbumin
keywords: Aggregate; Confocal laser scanning microscopy; Konjac glucomannan; Ovalbumin; Phase separation