title: Inhibitory effect of nuclear factor-κB decoy oligodeoxynucleotide on liver fibrosis through regulation of the epithelial-mesenchymal transition.
abstract: The epithelial-mesenchymal transition (EMT) has been recognized to occur during embryonic development, fibrosis, and tumor metastasis. Nuclear factor (NF)-κB plays a central role in mediating the inflammation and wound-healing responses during liver fibrogenesis. However, the involvement of NF-κB during EMT in liver cells remains unidentified. To develop a therapeutic approach to EMT during liver fibrosis, we examined the inhibition of transcription factor NF-κB, using a decoy oligodeoxynucleotide (ODN) strategy in liver fibrosis in vitro and in vivo. NF-κB decoy ODN contains consensus binding sequences of the NF-κB-binding site. NF-κB decoy ODN effectively suppresses transforming growth factor-β(1)-induced EMT in AML12 murine hepatocytes. Liver fibrosis induced by CCl(4) administration was suppressed by NF-κB decoy ODN. Furthermore, NF-κB decoy ODN was shown to inhibit the EMT process in fibrotic liver in vivo. This study demonstrates the feasibility of NF-κB decoy ODN treatment for preventing liver fibrosis via EMT processes.
authors: 1 Department of Pathology, Catholic University of Daegu , College of Medicine, 3056-6 Daemyung 4-dong, Nam-gu, Daegu 705-718, Republic of Korea.
authors: Kyung-Hyun
authors: KH
authors: Kim
authors: Woo-Ram
authors: WR
authors: Lee
authors: Yu-Na
authors: YN
authors: Kang
authors: Young-Chae
authors: YC
authors: Chang
authors: Kwan-Kyu
authors: KK
authors: Park
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D004247:DNA / Q000008:administration & dosage / Q000494:pharmacology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011485:Protein Binding; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000502:physiology
chemical_list: D016328:NF-kappa B; D053773:Transforming Growth Factor beta1; D004247:DNA