title: Collagen nanofibers facilitated presynaptic maturation in differentiated neurons from spinal-cord-derived neural stem cells through MAPK/ERK1/2-Synapsin I signaling pathway.
abstract: Neural stem cells (NSCs) are deemed to be a potential cell therapy for brain and spinal cord reconstruction and regeneration following injury. In this study, we investigated the role of nanofibrous scaffolds on NSCs-derived neurons in the formation of neural networks. Miniature excitatory postsynaptic currents (mEPSCs) were recorded using the whole-cell patch clamp recording method after the spinal cord-derived NSCs were differentiated into neurons and cultured in vitro for 10-14 days. It was observed that the frequency of mEPSCs in the differentiated neurons cultured on both randomly oriented and aligned collagen nanofibrous scaffolds was higher than that on the collagen-coated control and can be inhibited by an ERK inhibitor (PD98059), indicating that the collagen nanofibers affected the maturation of the synapses from presynaptic sites via the MAPK/ERK1/2 pathway. In addition, both of the collagen nanofibers increased the phosphorylation of Synapsin I and facilitated the interaction of p-ERK1/2 and p-Synapsin I. All these results suggested that the collagen nanofibrous scaffolds contributed to the presynaptic maturation via the ERK1/2-Synapsin I signaling pathway.
authors: Department of Neurobiology and Beijing Institute for Brain Disorders, School of Basic Medical Sciences, Capital Medical University , Beijing 100069, PR China.
authors: Yanling
authors: Y
authors: Yin
authors: Peng
authors: P
authors: Huang
authors: Zhu
authors: Z
authors: Han
authors: Guojun
authors: G
authors: Wei
authors: Changwei
authors: C
authors: Zhou
authors: Jian
authors: J
authors: Wen
authors: Bo
authors: B
authors: Su
authors: Xiaoqin
authors: X
authors: Wang
authors: Yansong
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D058953:Neural Stem Cells / Q000187:drug effects* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D019635:Neurosurgical Procedures; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects; D016704:Synapsins / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D005419:Flavonoids; D016704:Synapsins; D003094:Collagen; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one