title: Fabrication of polyion complex vesicles with enhanced salt and temperature resistance and their potential applications as enzymatic nanoreactors.
abstract: Integrating catalytic functions into polymeric vesicles through enzyme entrapment is appealing for bioreactor fabrication, yet there are critical issues regarding the regulation of solute transport through membranes and enzyme loading without denaturation. Polyion complex vesicles (PICsomes) with semipermeable membranes and the propensity to form in water can overcome these issues; however, cross-linking is required for sufficient physiological stability. Herein, we report the first successful fabrication of non-cross-linked PICsomes with sufficient stability at physiological salinity and temperature by tuning the hydrophobicity of the aliphatic side chains in the pendant group of the constituent polyelectrolytes. Dynamic light scattering and transmission electron microscopy revealed that the intervesicular fusion and disintegration of the PICsomes was prevented and a narrow distribution was maintained at physiological salinity and temperatures. Furthermore, their application as enzymatic nanoreactors was verified even in the presence of proteases. As such, the potential utility of the PICsomes in biomedical fields was established.
authors: Graduate School of Engineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors: Sayan
authors: S
authors: Chuanoi
authors: Yasutaka
authors: Y
authors: Anraku
authors: Mao
authors: M
authors: Hori
authors: Akihiro
authors: A
authors: Kishimura
authors: Kazunori
authors: K
authors: Kataoka
mesh_terms: D055598:Chemical Phenomena; D003185:Complement C8 / Q000737:chemistry; D004798:Enzymes / Q000737:chemistry; D007477:Ions / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry*; D013696:Temperature
chemical_list: D003185:Complement C8; D004798:Enzymes; D007477:Ions; D011108:Polymers; D012965:Sodium Chloride