title: PELP1 suppression inhibits colorectal cancer through c-Src downregulation.
abstract: Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1), a coregulator of estrogen receptors alpha and beta, is a potential protooncogene implicated in several human cancers, including sexual hormone-responsive or sexual hormone-nonresponsive cancers. However, the functions of PELP1 in colorectal cancer remain unclear. In this study, western blot and bioinformatics revealed that PELP1 expression was higher in several colorectal cancer cell lines than in immortalized normal colorectal epithelium. PELP1 silencing by short hairpin RNA promoted the senescence and inhibited the proliferation, colony formation, migration, invasion, and xenograft tumor formation of the CRC cell line HT-29. Moreover, PELP1 silencing was accompanied by c-Src downregulation. c-Src upregulation partly alleviated the damage in HT-29 malignant behavior induced by PELP1 RNA interference. In conclusion, PELP1 exhibits an oncogenic function in colorectal cancer through c-Src upregulation.
authors: Hubei Province Key Laboratory on Cardiovascular, Cerebrovascular, and Metabolic Disorders, Hubei University of Science and Technology, Xianning 437100, China ; The Basic Medical School, Hubei University of Science and Technology, Xianning 437100, China.
authors: Zhifeng
authors: Z
authors: Ning
authors: School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, China.
authors: Youzhi
authors: Y
authors: Zhang
authors: Wuhan Blood Center, Wuhan 430030, China.
authors: Hanwei
authors: H
authors: Chen
authors: Hubei Province Key Laboratory on Cardiovascular, Cerebrovascular, and Metabolic Disorders, Hubei University of Science and Technology, Xianning 437100, China.
authors: Jiliang
authors: J
authors: Wu
authors: The Basic Medical School, Hubei University of Science and Technology, Xianning 437100, China.
authors: Tieshan
authors: T
authors: Song
authors: The Basic Medical School, Hubei University of Science and Technology, Xianning 437100, China.
authors: Qian
authors: Q
authors: Wu
authors: The Basic Medical School, Hubei University of Science and Technology, Xianning 437100, China.
authors: Fuxing
authors: F
authors: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D056970:Co-Repressor Proteins / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D019295:Computational Biology; D015536:Down-Regulation; D006801:Humans; D008819:Mice, Nude; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation; D023041:Xenograft Model Antitumor Assays
chemical_list: D056970:Co-Repressor Proteins; C438485:PELP1 protein, human; D034741:RNA, Small Interfering; D014157:Transcription Factors; D016392:Proto-Oncogene Proteins pp60(c-src)