title: The effect of extra virgin olive oil and soybean on DNA, cytogenicity and some antioxidant enzymes in rats.
abstract: We investigated the effect of extra virgin (EV) olive oil and genetically modified (GM) soybean on DNA, cytogenicity and some antioxidant enzymes in rodents. Forty adult male albino rats were used in this study and divided into four groups. The control group of rodents was fed basal ration only. The second group was given basal ration mixed with EV olive oil (30%). The third group was fed basal ration mixed with GM (15%), and the fourth group survived on a combination of EV olive oil, GM and the basal ration for 65 consecutive days. On day 65, blood samples were collected from each rat for antioxidant enzyme analysis. In the group fed on basal ration mixed with GM soyabean (15%), there was a significant increase in serum level of lipid peroxidation, while glutathione transferase decreased significantly. Interestingly, GM soyabean increased not only the percentage of micronucleated polychromatic erythrocytes (MPCE), but also the ratio of polychromatic erythrocytes to normochromatic erythrocytes (PEC/NEC); however, the amount of DNA and NCE were significantly decreased. Importantly, the combination of EV olive oil and GM soyabean significantly altered the tested parameters towards normal levels. This may suggest an important role for EV olive oil on rodents' organs and warrants further investigation in humans.
authors: Department of Clinical Nutrition, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia. Thanaelkholy@hotmail.com.
authors: Thanaa A
authors: TA
authors: El-Kholy
authors: School of Medicine, University of Southampton, Southampton SO16 6YD, UK. abuhilal9@gmail.com.
authors: Mohammad
authors: M
authors: Abu Hilal
authors: Experimental Surgery Unit, KFMRC (King Fahd Medical Research Center), Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia. hatimalabbadi@yahoo.com.
authors: Hatim Ali
authors: HA
authors: Al-Abbadi
authors: Faculty of Medicine, Umm Al Qura University, Makkah 21955, Saudi Arabia. abdulhalims@hotmail.com.
authors: Abdulhalim Salim
authors: AS
authors: Serafi
authors: Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia. aalghamdi@kau.edu.sa.
authors: Ahmad K
authors: AK
authors: Al-Ghamdi
authors: Pharmacology Unit, Biochemistry, Toxicology and Food deficiency Department, Animal Health Research Institute, Dokki, Gizza, Egypt. dr.hanan_mss@yahoo.com.
authors: Hanan M
authors: HM
authors: Sobhy
authors: Surgical Registrar, University Hospital Southampton, Southampton SO16 6YD, UK. johnrcr@hotmail.com.
authors: John R C
authors: JR
authors: Richardson
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004032:Diet; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max
chemical_list: D000975:Antioxidants; D000069463:Olive Oil; D010938:Plant Oils; D004247:DNA