title: The effect of maternal nicotine on basement membrane collagen IV of brain microvessels changes in neonatal Balb/C mice.
abstract: BACKGROUND
  Nicotine can pass through placental blood barrier and accumulate in the developing organs of fetus. Also, entering the breast milk, nicotine can have an effect on the neonates. Investigations have showed that collagen IV is one of the most important micro vessels basement membrane components.
  
  
  OBJECTIVE
  In this study, the effect of maternal nicotine exposure in pre and postnatal periods on collagen IV in microvessels of neonatal Balb/C mice brain cortex was studied by immunohistochemistry technique.
  
  
  METHODS
  24 pregnant Balb/C mice were divided in to 4 groups (6 mice in each group): two experimental and 2 control groups. The mothers in the 1(st) experimental group were injected 3 mg/kg nicotine intrapritoneally from the 5(th) day of pregnancy to parturition daily and in 2(nd) experimental group the same procedure was repeated to the 10(th) day after parturition (lactation). The control groups received the same volume of normal saline during the same time. 10 days after delivery, the brain tissues of newborns were isolated. Then, prepared blocks from fixed brain were cut serially for immunohistochemical assay.
  
  
  RESULTS
  The findings of the present study indicated that collagen IV reaction in microvessels basement membrane in the first experimental group increased significantly compared to the first control group (p=0.002). In addition, collagen IV reaction in microvessels basement membrane in the 2(nd) experimental group increased significantly compared to the 2(nd) control group (p=0.002). However, no significant difference was observed between the two experimental groups.
  
  
  CONCLUSIONS
  These results suggested that maternal nicotine exposure during prenatal period may increase basement membrane collagen IV expression. Also, nicotine increases in maternal breast milk has no effect on basement membrane collagen IV expression.
authors: Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors: Somayyeh Sadat
authors: SS
authors: Tahajjodi
authors: Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors: Maryam
authors: M
authors: Amerion
authors: Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors: Nasser
authors: N
authors: Mahdavi Shahri
authors: Department of Anatomy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Mehdi
authors: M
authors: Jalali
authors: Department of Anatomy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Mohammad Reza
authors: MR
authors: Nikravesh
keywords: Basement membrane; Brain microvessels; Collagen type IV; Nicotine