title: MicroRNA124 regulate cell growth of prostate cancer cells by targeting iASPP.
abstract: Protein phosphatase 1, regulatory subunit 13 like PPP1R13L, also coined iASPP, was found high expression in prostate cancer tissues and cell lines. In previous research, in vitro and in vivo RNAi mediated by artificial lentiviral shRNAs which proved that suppression of iASPP decrease the proliferation of cancer cells. Endogenous interference RNAs, microRNAs play key roles in cell proliferation by post-transcriptional regulation of gene expression. Natural base pair matched microRNA for iASPP is mir124, which was found high expression in growth factorloss prostate cancer cell lines. In this study we examined effect of mir124 upon iASPP and proliferation of prostate cells in vitro with lentiviral infection and use artificial shRNA as control. In vitro reporter assay confirmed that mir124 binding the 3'UTR of iASPP and suppress mRNA expression. Lentivirus mediated mir124 expression decreased the proliferation and viability of PC3 while endogenous iASPP were knocked down.
authors: Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University Guangzhou 510006, P. R. China ; Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630, P. R. China.
authors: Jun
authors: J
authors: Chen
authors: Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630, P. R. China.
authors: Hengjun
authors: H
authors: Xiao
authors: Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630, P. R. China.
authors: Zhansen
authors: Z
authors: Huang
authors: Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630, P. R. China.
authors: Zhiming
authors: Z
authors: Hu
authors: Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630, P. R. China.
authors: Tao
authors: T
authors: Qi
authors: Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630, P. R. China.
authors: Bin
authors: B
authors: Zhang
authors: Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630, P. R. China.
authors: Xin
authors: X
authors: Tao
authors: Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University Guangzhou 510006, P. R. China.
authors: Song-Hao
authors: SH
authors: Liu
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D016086:Lentivirus / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D047908:Intracellular Signaling Peptides and Proteins; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; C119612:PPP1R13L protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012097:Repressor Proteins
keywords: Mir124; cell growth; iASPP; prostate cancer