title: Misidentification of Candida guilliermondii as C. famata among strains isolated from blood cultures by the VITEK 2 system.
abstract: Introduction. The aim of this study was to differentiate between Candida famata and Candida guilliermondii correctly by using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and gene sequencing. Methods. Twenty-eight Candida strains from blood cultures that had been identified as C. famata (N = 25), C. famata/C. guilliermondii (N = 2), and C. guilliermondii (N = 1) by the VITEK 2 system using the YST ID card were included. We identified these strains by MALDI-TOF MS and gene sequencing using the 28S rRNA and ITS genes and compared the results with those obtained by the VITEK 2 system. Results. All 28 isolates were finally identified as C. guilliermondii. Sequencing analysis of the 28S rRNA gene showed 99.80%-100% similarity with C. guilliermondii for all 28 strains. The ITS gene sequencing of the strains showed 98.34%-100% homology with C. guilliermondii. By MALDI-TOF, we could correctly identify 21 (75%) of 28 C. guilliermondii isolates. Conclusion. We should suspect misidentification when C. famata is reported by the VITEK 2 system, and we always should keep in mind the possibility of misidentification of any organism when an uncommon species is reported.
authors: Department of Laboratory Medicine, Inje University College of Medicine, Busan 614-735, Republic of Korea ; Paik Institute for Clinical Research, Inje University College of Medicine, Busan 614-735, Republic of Korea.
authors: Si Hyun
authors: SH
authors: Kim
authors: Department of Laboratory Medicine, Inje University College of Medicine, Busan 614-735, Republic of Korea ; Paik Institute for Clinical Research, Inje University College of Medicine, Busan 614-735, Republic of Korea.
authors: Jeong Hwan
authors: 0000-0003-3960-6969
authors: JH
authors: Shin
authors: Department of Internal Medicine, Pusan National University School of Medicine, Busan 602-739, Republic of Korea ; Biomedical Research Institute, Pusan National University School of Medicine, Busan 602-739, Republic of Korea.
authors: Jeong Ha
authors: JH
authors: Mok
authors: Department of Laboratory Medicine, Pusan National University School of Medicine, Busan 602-739, Republic of Korea.
authors: Shine Young
authors: SY
authors: Kim
authors: Department of Laboratory Medicine, Inje University College of Medicine, Busan 614-735, Republic of Korea.
authors: Sae Am
authors: SA
authors: Song
authors: Department of Laboratory Medicine, Inje University College of Medicine, Busan 614-735, Republic of Korea.
authors: Hye Ran
authors: HR
authors: Kim
authors: Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea.
authors: Joong-Ki
authors: JK
authors: Kook
authors: Korean Collection for Type Cultures, Biological Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors: Young-Hyo
authors: YH
authors: Chang
authors: Department of Dental Hygiene, College of Medical and Life Science, Silla University, Busan 617-736, Republic of Korea.
authors: Il Kwon
authors: IK
authors: Bae
authors: Department of Internal Medicine, Pusan National University School of Medicine, Busan 602-739, Republic of Korea ; Biomedical Research Institute, Pusan National University School of Medicine, Busan 602-739, Republic of Korea ; Department of Internal Medicine, Pusan National University Hospital, 179, Gudeok-ro, Seo-gu, Busan 602-739, Republic of Korea.
authors: Kwangha
authors: 0000-0001-9878-201X
authors: K
authors: Lee
mesh_terms: D000465:Algorithms; D002175:Candida / Q000145:classification* / Q000235:genetics; D002177:Candidiasis / Q000097:blood / Q000382:microbiology*; D019411:Clinical Laboratory Techniques; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D003951:Diagnostic Errors; D006801:Humans; D016533:Mycological Typing Techniques; D010641:Phenotype; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S