title: bta-miR-29b attenuates apoptosis by directly targeting caspase-7 and NAIF1 and suppresses bovine viral diarrhea virus replication in MDBK cells.
abstract: MicroRNAs (miRNAs) are small, endogenous, noncoding RNA molecules that serve as powerful regulators of multiple cellular processes, including apoptosis, differentiation, growth, and proliferation. Bovine viral diarrhea virus (BVDV) contributes significantly to health-related economic losses in the beef and dairy industries. Although BVDV-induced apoptosis correlates with increased intracellular viral RNA accumulation and with bta-miR-29b (miR-29b) expression upregulation in Madin-Darby bovine kidney (MDBK) cells infected with BVDV strain NADL, the role of miR-29b in regulating BVDV-infection-related apoptosis remains unexplored. Here, we report that miR-29b serves as a new miRNA regulating apoptosis. We showed that miR-29b target sequences were present in the 3' untranslated regions of 2 key apoptosis regulators mRNAs, cysteine aspartases-7 (caspase-7) and nuclear apoptosis-inducing factor 1 (NAIF1). Indeed, upon miRNA overexpression, both mRNA and protein levels of caspase-7 and NAIF1 were decreased. We further found that miR-29b attenuated apoptosis by directly regulating intracellular levels of caspase-7 and NAIF1. Moreover, apoptosis blockage by miR-29b was rescued upon co-infection of MDBK cells with lentiviruses expressing caspase-7 and NAIF1. Importantly, miR-29b decreased BVDV NADL envelope glycoprotein E1 mRNA levels and suppressed viral replication. These studies advance our understanding of the mechanisms of miRNAs in mediating the cells combating viral infections.
authors: a College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, People's Republic of China.
authors: Qiang
authors: Q
authors: Fu
authors: Huijun
authors: H
authors: Shi
authors: Mengting
authors: M
authors: Shi
authors: Luping
authors: L
authors: Meng
authors: Hui
authors: H
authors: Zhang
authors: Yan
authors: Y
authors: Ren
authors: Fei
authors: F
authors: Guo
authors: Bin
authors: B
authors: Jia
authors: Pengyan
authors: P
authors: Wang
authors: Wei
authors: W
authors: Ni
authors: Chuangfu
authors: C
authors: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D051033:Apoptosis Inducing Factor / Q000235:genetics* / Q000378:metabolism; D053179:Caspase 7 / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000502:physiology; D012367:RNA, Viral / Q000235:genetics; D015854:Up-Regulation; D014779:Virus Replication / Q000235:genetics*
chemical_list: D051033:Apoptosis Inducing Factor; D035683:MicroRNAs; D009687:Nuclear Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D053179:Caspase 7
keywords: NAIF1; apoptose; apoptosis; bovine viral diarrhea virus; caspase-7; miR-29b; virus de la diarrhée virale bovine