title: Caco-2 replace or refine?
abstract: Multiple screening techniques have been developed to gain simplicity and rapidness in prediction of human intestinal permeability. The most extensively used method for years has been the Caco-2 cell monolayers. Are the less time- and resource-consuming artificial membranes and computational-based predictions on their way to replace the Caco-2 cells? The importance of mechanistic approaches and correct interpretation of the data using the techniques available is compared and discussed in this article.:
authors: DMPK and Bioanalytical Chemistry, AstraZeneca R&D, S-431 83 Mölndal, Sweden. Electronic address:anna-lena.ungell@astrazeneca.com.
authors: Anna-Lena B
authors: AL
authors: Ungell