title: [Lack of association between the S447X variant of the lipoprotein lipase gene and plasma lipids. A preliminary study].
abstract: The increase in lipid plasma values is an important cardiovascular risk factor. Lipoprotein lipase (LPL) plays an important role in the lipoprotein metabolism and metabolic and genetic factors may influence its levels and functions. The S447X variant of the lipoprotein lipase gene is associated with changes in plasma lipids in different populations. The objective of this research was to analyze the S447X variant of the LPL gene and its relation with plasma lipids of individuals in Zulia state, Venezuela. With this purpose, we studied 75 individuals (34 men and 41 women) between 20 and 60 years of age. Each subject had a medical history which included family history, anthropometric characteristics, nutritional status evaluation and biochemical tests. Genomic DNA was extracted for the molecular study and the polymerase chain reaction was used, followed by enzyme digestion, for restriction fragments length polymorphisms using the Hinf I enzyme. The individuals studied had normal levels of blood glucose, triglycerides, total cholesterol and low density lipoproteins (LDL-C) and slightly decreased levels of high density lipoproteins (HDL-C). The genotypic distribution of the LPL gene S447X variant in the studied population was 90.6% for the homozygous genotype SS447 and 9.4% for the heterozygote SX447. The genotype 447XX was not identified. The population was found in Hardy Weinberg genetic equilibrium. No association between the S447X polymorphism of lipoprotein lipase gene and plasma lipids was observed.
authors: Mariana
authors: M
authors: Zambrano Morales
authors: Erika
authors: E
authors: Fernández Salgado
authors: Ligia
authors: L
authors: Balzán Urdaneta
authors: Neila
authors: N
authors: Labastidas
authors: José
authors: J
authors: Aranguren-Méndez
authors: Lissette
authors: L
authors: Connell
authors: Tania
authors: T
authors: Molero Paredes
authors: Alicia
authors: A
authors: Rojas
authors: Amelia
authors: A
authors: Panunzio
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D004252:DNA Mutational Analysis; D005260:Female; D005838:Genotype; D006801:Humans; D007328:Insulin / Q000032:analysis; D008055:Lipids / Q000097:blood*; D008071:Lipoprotein Lipase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D014687:Venezuela; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D008071:Lipoprotein Lipase