title: [Antitumor efficacy of the recombinant Newcastle disease virus rNDV-IL15 on melanoma models].
abstract: In order to enhance the antitumor efficacy of recombinant Newcastle disease virus, rNDV-IL15 was rescued in this study. Recombinant plasmid prNDV-IL15 was constructed, and BHK21 cells were transfected with the recombinant plasmid. Finally, the recombinant Newcastle disease virus rNDV-IL15 was successfully rescued. The growth curves of these two recombinant viruses were determined. Murine melanoma B16F10 cells were infected with rNDV-IL15 at MOI of 0.1, and the expression level of IL15 in the supernatant was detected by ELISA. The antitumor efficacy of rNDV-IL15 and rNDV was compared in vitro and in vivo. Results showed that prNDV-IL15 was constructed and recombinant virus rNDV-IL15 was successfully rescued. The growth curve of rNDV-IL15 showed that the growth of rNDV-IL15 had not been changed after insertion of IL15 gene. Results showed that there was high level of IL15 expression in the supernatant of rNDV-IL5-infected B16F10 cells (1 044.3 +/- 27.7 ng x mL(-1)). rNDV-IL15 and rNDV significantly inhibited the growth of B16F10 cells in vitro in a time-dependent manner. However, there was no significant difference between them. In animal experiments, rNDV-IL15 efficiently suppressed tumor growth in vivo when compared with rNDV, and the difference was statistically significant. The results suggested that rNDV-IL15 is a more effective antitumor agent.
authors: Ze-Shan
authors: ZS
authors: Niu
authors: Fu-Liang
authors: FL
authors: Bai
authors: Tian
authors: T
authors: Sun
authors: Hui
authors: H
authors: Tian
authors: Jie-Chao
authors: JC
authors: Yin
authors: Hong-Wei
authors: HW
authors: Cao
authors: Dan
authors: D
authors: Yu
authors: Gui-You
authors: GY
authors: Tian
authors: Yun-Zhou
authors: YZ
authors: Wu
authors: De-Shan
authors: DS
authors: Li
authors: Gui-Ping
authors: GP
authors: Ren
mesh_terms: D000818:Animals; D001835:Body Weight; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002642:Chick Embryo; D003602:Cytotoxicity, Immunologic; D005260:Female; D015316:Genetic Therapy; D019409:Interleukin-15 / Q000235:genetics* / Q000378:metabolism; D008546:Melanoma, Experimental / Q000473:pathology* / Q000628:therapy; D051379:Mice; D009368:Neoplasm Transplantation; D009522:Newcastle disease virus / Q000235:genetics*; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D047368:Tumor Burden
chemical_list: D019409:Interleukin-15; D011994:Recombinant Proteins