title: High prevalence of blaCTX-M-1/IncI1/ST3 and blaCMY-2/IncI1/ST2 plasmids in healthy urban dogs in France.
abstract: In the community, close contacts between humans and dogs may promote the transfer of extended-spectrum beta-lactamase/plasmidic AmpC cephalosporinase (ESBL/pAmpC) genes. Large-scale prevalence studies on ESBL/pAmpC carriage in dogs are rare, and data on ESBL/pAmpC plasmids are even more limited. Here, a considerable rate of 18.5% ESBL/pAmpC carriers was found among 368 unrelated healthy dogs in Paris, France. This prevalence is much higher than the one found in healthy humans in the same city (6%) but close to that recently reported in dogs in China (24.5%). All isolates were identified as Escherichia coli, except one Salmonella enterica and one Klebsiella pneumoniae isolate. The sequence type 131 (ST131) clone was rare (2/73 isolates). Interestingly, two plasmids (blaCTX-M-1/IncI1/ST3 and blaCMY-2/IncI1/ST2) were unexpectedly highly predominant, raising the question of their successful spread. Considering that CTX-M-1 was recently found to be equally as abundant as CTX-M-15 in healthy Parisian subjects, the question of dogs being a CTX-M-1 reservoir for humans is open. Such a high prevalence of the blaCMY-2/IncI1/ST2 plasmid may result from the use of cephalexin in veterinary medicine, as previously demonstrated experimentally. In all, our study points out healthy urban dogs as a potential source of ESBL/pAmpC genes that can further disseminate to the human community.
authors: Agence Nationale de Sécurité Sanitaire, Unité Antibiorésistance et Virulence Bactériennes, Lyon, France marisa.haenni@anses.fr.
authors: Marisa
authors: M
authors: Haenni
authors: Agence Nationale de Sécurité Sanitaire, Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
authors: Estelle
authors: E
authors: Saras
authors: Agence Nationale de Sécurité Sanitaire, Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
authors: Véronique
authors: V
authors: Métayer
authors: Vebio, Laboratoire d'Analyses Vétérinaires, Arcueil, France.
authors: Christine
authors: C
authors: Médaille
authors: Agence Nationale de Sécurité Sanitaire, Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
authors: Jean-Yves
authors: JY
authors: Madec
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D002510:Cephalosporinase / Q000235:genetics; D002681:China; D004285:Dogs; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests / Q000379:methods; D010297:Paris; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification*; D015995:Prevalence; D019779:Salmonella enterica / Q000235:genetics / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002510:Cephalosporinase; C055184:beta-lactamase TEM-3; C048795:AmpC beta-lactamases; D001618:beta-Lactamases