title: Optimization of direct currents to enhance dentine bonding of simplified one-step adhesive.
abstract: The aim of this study was to investigate the effects of different direct current intensities on dentine bonding effectiveness of Clearfil S(3) Bond and on cell viability of human dental pulp cells (HDPCs). Thirty-five-third molars were sectioned and ground to provide flat surfaces. Clearfil S(3) Bond was applied under different current conditions for 30 s and then resin composite was built up. Specimens were processed for microtensile bond strength (µTBS) testing and for nanoleakage investigation using scanning electron microscopy. Primary HDPCs isolated from premolars were stimulated with different intensities of electric current for 30 s. Then, cell viability was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Specimens bonded with application of electrical current intensities of 50, 60, 70, and 90 µA exhibited a significant increase in immediate µTBS compared with all other groups. Bonded interfaces prepared using electrically assisted current application showed reduced interfacial nanoleakage upon scanning electron microscopy. Electric current application, from 20 to 70 µA, had no effect on the viability of HDPCs. This study provides further evidence for its future clinical use.
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors: Hongfei
authors: H
authors: Chen
authors: Dongjie
authors: D
authors: Fu
authors: Hongye
authors: H
authors: Yang
authors: Yinchen
authors: Y
authors: Liu
authors: Yanyu
authors: Y
authors: Huang
authors: Cui
authors: C
authors: Huang
mesh_terms: D000293:Adolescent; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004396:Coloring Agents; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D003763:Dental Leakage / Q000145:classification; D003764:Dental Materials / Q000737:chemistry; D003782:Dental Pulp / Q000166:cytology; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004558:Electric Stimulation; D004560:Electricity; D055664:Electrochemical Techniques; D006801:Humans; D008855:Microscopy, Electron, Scanning; D019279:Resin Cements / Q000737:chemistry*; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: C505694:Clearfil S3 Bond; D004396:Coloring Agents; D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; D019279:Resin Cements; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue
keywords: bonding effectiveness; cell viability; dentine bonding; electric-assisted device; human dental pulp cells