title: Positive cross talk between FOXL2 and antimüllerian hormone regulates ovarian reserve.
abstract: OBJECTIVE
  To demonstrate interregulation between FOXL2 and antimüllerian hormone (AMH) in ovarian folliculogenesis.
  
  
  METHODS
  Cell culture and animal study.
  
  
  METHODS
  University research laboratory.
  
  
  METHODS
  Five-week-old B6C3F1 mice.
  
  
  METHODS
  Molecular analysis and in vivo mouse experiment were performed to demonstrate that AMH is a target gene of FOXL2 in the ovary.
  
  
  METHODS
  To determine whether FOXL2 transactivates AMH, luciferase reporter assay, electrophoretic mobility shift assay, and chromatin immuniprecipitation were conducted. Using an in vivo nucleic acid delivery system, the expression of AMH and/or FOXL2 was modulated in the mouse, and the ovaries were histologically analyzed.
  
  
  RESULTS
  AMH is an endogenous target gene of FOXL2. In contrast, mutated FOXL2s found in premature ovarian failure patients were defective in their ability to activate AMH transcription in human granulosa cells. In vivo mouse gene delivery experiments revealed that Amh-knockdown accelerated follicle growth; however, the acceleration was prevented by ectopic expression of FOXL2.
  
  
  CONCLUSIONS
  AMH and FOXL2 collaboratively work to reserve ovarian follicles.
authors: Department of Life Science, Chung-Ang University, Seoul, South Korea.
authors: Mira
authors: M
authors: Park
authors: Department of Obstetrics and Gynecology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
authors: Dae-Shik
authors: DS
authors: Suh
authors: Department of Life Science, Chung-Ang University, Seoul, South Korea.
authors: Kangseok
authors: K
authors: Lee
authors: School of Pharmacy, Chung-Ang University, Seoul, South Korea. Electronic address: jeehyeon@cau.ac.kr.
authors: Jeehyeon
authors: J
authors: Bae
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000235:genetics / Q000502:physiology*; D002452:Cell Count; D002478:Cells, Cultured; D005260:Female; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D016649:Primary Ovarian Insufficiency / Q000378:metabolism
chemical_list: D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors; C498893:Foxl2 protein, mouse; D054304:Anti-Mullerian Hormone
keywords: Folliculogenesis; ovarian reserve; transactivation