title: Somatostatin regulates tight junction proteins expression in colitis mice.
abstract: Tight junction plays a critical role in intestinal defence. The alteration and perturbation of tight junction proteins could induce intestine barrier damage, and lead to the malabsorption of electrolytes and water. Previous studies had showed that colonic infection and inflammation could lead to the alteration of tight junction function, and somatostatin could protect intestinal epithelia. Thus, this study could explore that whether somatostatin could regulate tight junction in colitis mice. Colitis mice with diarrhea were induced by Citrobacter rodentium (CR) and Dextran sulfate sodium (DSS). In CR infected model, cladudin-1 and claudin-3 expression significantly decreased compared with the control mice (P<0.05); after octreotide treatment, claudin-1 and claudin-3 expression significantly increased compared with untreated CR infected mice (P<0.05). In DSS colitis model, occludin and claudin-3 expression significantly decreased compared with the control mice (P<0.05); and octreotide treatment could only significantly upregulate claudin-3 expression compared with untreated DSS colitis mice (P<0.05). To testify our results in vivo, we repeated the models in caco-2 cells by exposed with enteropathogenic Escherichia coli (E. Coli) and Tumor necrosis factor α (TNF-α). The results in vitro were consistent with in vivo study. The results suggested that somatostatin play a role in intestinal barrier protection by modulating tight junction proteins expression.
authors: Department of Gastroenterology, West China Hospital of Sichuan University GuoXue Street 37, Chengdu, China.
authors: Xiao
authors: X
authors: Li
authors: Department of Gastroenterology, West China Hospital of Sichuan University GuoXue Street 37, Chengdu, China.
authors: Qian
authors: Q
authors: Wang
authors: University of Arizona Health Science Center Tucson, Arizona.
authors: Hua
authors: H
authors: Xu
authors: Department of Gastroenterology, West China Hospital of Sichuan University GuoXue Street 37, Chengdu, China.
authors: Liping
authors: L
authors: Tao
authors: Department of Gastroenterology, West China Hospital of Sichuan University GuoXue Street 37, Chengdu, China.
authors: Jing
authors: J
authors: Lu
authors: Department of Gastroenterology, West China Hospital of Sichuan University GuoXue Street 37, Chengdu, China.
authors: Lin
authors: L
authors: Cai
authors: Department of Gastroenterology, West China Hospital of Sichuan University GuoXue Street 37, Chengdu, China.
authors: Chunhui
authors: C
authors: Wang
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D044082:Citrobacter rodentium / Q000472:pathogenicity; D062445:Claudin-1 / Q000378:metabolism; D062465:Claudin-3 / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D016264:Dextran Sulfate; D003967:Diarrhea / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000472:pathogenicity; D005260:Female; D005765:Gastrointestinal Agents / Q000494:pharmacology*; D006801:Humans; D008810:Mice, Inbred C57BL; D015282:Octreotide / Q000494:pharmacology*; D013004:Somatostatin / Q000031:analogs & derivatives / Q000494:pharmacology*; D062725:Tight Junction Proteins / Q000378:metabolism*; D019108:Tight Junctions / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D062445:Claudin-1; D062465:Claudin-3; C568113:Cldn1 protein, mouse; C568123:Cldn3 protein, mouse; D005765:Gastrointestinal Agents; D062725:Tight Junction Proteins; D014409:Tumor Necrosis Factor-alpha; D013004:Somatostatin; D016264:Dextran Sulfate; D015282:Octreotide
keywords: IBD; Tight junction; claudins; infectious colitis; occludin; somatostatin