title: Age-dependent association of mannose-binding lectin polymorphisms with the development of pulmonary tuberculosis in Viet Nam.
abstract: Mannose-binding lectin (MBL) binds to pathogens and induces complement-mediated opsonophagocytosis. Although the association between MBL2 polymorphisms and tuberculosis (TB) has been studied in various populations, the results are controversial. We explored the stages of TB associated with MBL2 polymorphisms. X/Y (rs7096206) and A/B (rs1800450) were genotyped in 765 new patients with active pulmonary TB without HIV infection and 556 controls in Hanoi, Viet Nam. The MBL2 nucleotide sequences were further analyzed, and plasma MBL levels were measured in 109 apparently healthy healthcare workers and 65 patients with TB. Latent TB infection (LTBI) was detected by interferon-gamma release assay (IGRA). The YA/YA diplotype, which exhibited high plasma MBL levels, was associated with protection against active TB in younger patients (mean age = 32)≦ 45 years old (odds ratio, 0.61; 95% confidence interval, 0.46-0.80). The resistant diplotype was less frequently found in the younger patients at diagnosis (P = 0.0021). MBL2 diplotype frequencies and plasma MBL levels were not significantly different between the IGRA-positive and -negative groups. MBL2 YA/YA exhibited a protective role against the development of TB in younger patients, whereas the MBL2 genotype and MBL levels were not associated with LTBI. High MBL levels may protect against the early development of pulmonary TB after infection.
authors: Department of Pathophysiology and Host Defense, The Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo 204-8533, Japan; National Center for Global Health and Medicine, Tokyo 162-8655, Japan.
authors: Minako
authors: M
authors: Hijikata
authors: Department of Pathophysiology and Host Defense, The Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo 204-8533, Japan.
authors: Ikumi
authors: I
authors: Matsushita
authors: NCGM-BMH Medical Collaboration Center, Hanoi, Viet Nam.
authors: Nguyen Thi Le
authors: NT
authors: Hang
authors: Department of Mycobacterium Reference and Research, The Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo 204-8533, Japan.
authors: Shinji
authors: S
authors: Maeda
authors: Hanoi Lung Hospital, Hanoi, Viet Nam.
authors: Pham Huu
authors: PH
authors: Thuong
authors: Department of Biochemistry, Hematology and Blood Transfusion, Hanoi Lung Hospital, Hanoi, Viet Nam.
authors: Do Bang
authors: do B
authors: Tam
authors: Department of Clinical Study and Informatics, Center for Clinical Sciences, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.
authors: Takuro
authors: T
authors: Shimbo
authors: National Center for Global Health and Medicine, Tokyo 162-8655, Japan.
authors: Shinsaku
authors: S
authors: Sakurada
authors: Hanoi Lung Hospital, Hanoi, Viet Nam.
authors: Vu Cao
authors: VC
authors: Cuong
authors: Hanoi Department of Health, Hanoi, Viet Nam.
authors: Luu Thi
authors: LT
authors: Lien
authors: Department of Pathophysiology and Host Defense, The Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo 204-8533, Japan; National Center for Global Health and Medicine, Tokyo 162-8655, Japan. Electronic address: nkeicho-tky@umin.ac.jp.
authors: Naoto
authors: N
authors: Keicho
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D006239:Haplotypes; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D055985:Latent Tuberculosis / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics* / Q000276:immunology; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014397:Tuberculosis, Pulmonary / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D014744:Vietnam
chemical_list: C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin; D007371:Interferon-gamma
keywords: Genetic association; Latent tuberculosis infection; Mannose-binding lectin; Polymorphism; Pulmonary tuberculosis