title: Morphological changes of cerebral vessels and expression patterns of MMP-2 and MMP-9 on cerebrovascular wall of alcoholic rats.
abstract: Alcohol abuse increases the incidence of cerebral accidents, which correlates with cerebrovascular structural changes. The present study was designed to observe the cerebrovascular remodeling of drinking rats with light microscopy and transmission electron microscopy (TEM). Short-term alcohol administration induced apparent amplification of perivascular spaces around small vessels in brain tissue, while long-term administration caused pathological changes of basilar arteries (BAs), including endothelial exfoliation, inner elastic lamina (IEL) fragmentation and thickening of tunica media and adventitia. In addition, the relationship between cerebrovascular remodeling and MMP-2 and MMP-9 synthesized by endothelial cells and vascular smooth muscle cells was explored by immunohistochemistry. The two protein expression in cerebral vessels changed dynamically, peaking at 1-2 weeks after treatment, and decreasing as treatment continued. These results suggest that MMP-2 and MMP-9 may play a significant role in blood-brain barrier disruption after alcohol abuse. But the chronic changes of cerebral arteries resulted from drinking are not coincident with time course of MMP-2 and MMP-9 expression in situ.
authors: Institute of Basic Medicine, Hebei Medical University Shijiazhuang 050017, China.
authors: Qian
authors: Q
authors: Qi
authors: Institute of Basic Medicine, Hebei Medical University Shijiazhuang 050017, China.
authors: Xia
authors: X
authors: Liu
authors: Institute of Basic Medicine, Hebei Medical University Shijiazhuang 050017, China.
authors: Guozhong
authors: G
authors: Zhang
authors: Institute of Basic Medicine, Hebei Medical University Shijiazhuang 050017, China.
authors: Wenjing
authors: W
authors: He
authors: Institute of Basic Medicine, Hebei Medical University Shijiazhuang 050017, China.
authors: Rufei
authors: R
authors: Ma
authors: Institute of Basic Medicine, Hebei Medical University Shijiazhuang 050017, China.
authors: Bin
authors: B
authors: Cong
authors: Institute of Basic Medicine, Hebei Medical University Shijiazhuang 050017, China.
authors: Yingmin
authors: Y
authors: Li
mesh_terms: D000428:Alcohol Drinking; D000437:Alcoholism / Q000150:complications*; D000818:Animals; D001488:Basilar Artery / Q000201:enzymology / Q000648:ultrastructure; D001794:Blood Pressure; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000201:enzymology / Q000648:ultrastructure; D002478:Cells, Cultured; D002561:Cerebrovascular Disorders / Q000201:enzymology / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000201:enzymology / Q000648:ultrastructure; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000201:enzymology / Q000648:ultrastructure; D032389:Myocytes, Smooth Muscle / Q000201:enzymology / Q000648:ultrastructure; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D066253:Vascular Remodeling
chemical_list: C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat
keywords: Alcohol abuse; MMP-2; MMP-9; basilar artery; remodeling