title: Snail plays an oncogenic role in glioblastoma by promoting epithelial mesenchymal transition.
abstract: BACKGROUND
  The factors affecting glioblastoma progression are of great clinical importance since dismal outcomes have been observed for glioblastoma patients. The Snail gene is known to coordinate the regulation of tumor progression in diverse tumors through induction of epithelial mesenchymal transition (EMT); however, its role in glioblastoma is still uncertain. Therefore, we aimed to further define its role in vitro.
  
  
  RESULTS
  The small interfering RNA (siRNA) technique was employed to knock down Snail expression in three glioblastoma cell lines (KNS42, U87, and U373). Specific inhibition of Snail expression increased E-cadherin expression but decreased vimentin expression in all cell lines. In addition, inhibition of the expression of Snail significantly reduced the proliferation, viability, invasion, and migration of glioblastoma cells as well as increased the number of cells in the G1 phase.
  
  
  CONCLUSIONS
  Knockdown of Snail suppresses the proliferation, viability, migration, and invasion of cells as well as inhibits cell cycle progression by promoting EMT induction. The findings suggest that expression of this gene facilitates glioblastoma progression. Therefore, these results indicate the clinical significance of Snail for use as a potential therapeutic target for glioblastoma.
authors: Department of Pathology, College of Medicine, Seoul National University Hospital Seoul, Korea.
authors: Jae Kyung
authors: JK
authors: Myung
authors: Division of Pediatric Neurosurgery, Pediatric Clinical Neuroscience Center, Seoul National University Children's Hospital Seoul, Korea.
authors: Seung Ah
authors: SA
authors: Choi
authors: Division of Pediatric Neurosurgery, Pediatric Clinical Neuroscience Center, Seoul National University Children's Hospital Seoul, Korea.
authors: Seung-Ki
authors: SK
authors: Kim
authors: Division of Pediatric Neurosurgery, Pediatric Clinical Neuroscience Center, Seoul National University Children's Hospital Seoul, Korea.
authors: Kyu-Chang
authors: KC
authors: Wang
authors: Department of Pathology, College of Medicine, Seoul National University Hospital Seoul, Korea ; Neuroscience Institute, College of Medicine, Seoul National University Seoul, Korea.
authors: Sung-Hye
authors: SH
authors: Park
mesh_terms: D015703:Antigens, CD; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D018450:Disease Progression; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness; D009857:Oncogenes; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D000071250:Snail Family Transcription Factors; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D012333:RNA, Messenger; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D014746:Vimentin
keywords: Epithelial mesenchymal transition (EMT); Snail; glioblastoma; small interfering RNA (siRNA)