title: [Experimental research in vitro of TK/GCV system for osteosarcoma MG-63 cell damage].
abstract: OBJECTIVE
  To study the killing effects of the liposome-mediated thymidine kinase (TK)/ganciclovir (GCV) system on MG-63 osteosarcoma (OS) cells and its bystander effects.
  
  
  METHODS
  Liposome-mediated TK gene transfected into MG-63 OS cells, the efficiency of transfection was analyzed by flow cytometry and observed under inverted fluorescence microscope. Non-transfected osteosarcoma MG-63 cells were divided into three groups,in the experimental group 1 transfected TK/GCV cells cultured in solutiona liquid mixture by supernatant by 1/10,1/7,1/5,1/2 ratio to original broth; in the experimental group 2 transfected cells cultured in solutiona liquid mixture of supernatant filtered through 0.22 microm filter by 1/10,1/7, 1/5, 1/2 ratio to original broth, in control group the transfection cells cultured in original culture solution. Cell growth inhibition rate and osteosarcoma cell sensitivity to TK/GCV system were measured by MTT assay in each group.
  
  
  RESULTS
  The TK gene was transfected into MG-63 OS cells successfully by liposome-mediated, flow cytometry instrument detection TK gene transfection cell transfection efficiency can reach 75.5%. Six days later the MTT assay showed that in the experimental group 1 inhibition rate of all concentration ratio of the mixed culture fluid were statistically significant as compared with the control group (P < 0.05), and in the experimental group 2 that of the 1/10 and 1/7 of concentration ratio of mixed culture medium was not statistically significant as compared with the control group (P > 0.05). TK gene transfected MG-63 cells increased with the the GCV concentration,the cell apoptosis rate increased.
  
  
  CONCLUSIONS
  The experiment demonstrated that the MG-63 OS cells are sensitive to the liposome-mediated TK/GCV system and bystander effects are significant.
authors: Hua-Dong
authors: HD
authors: Zhang
authors: Zhi
authors: Z
authors: Lu
authors: Yi
authors: Y
authors: Feng
authors: Xiao-Li
authors: XL
authors: Liu
authors: Hui-Ming
authors: HM
authors: Hou
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology; D024201:Bystander Effect / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D015774:Ganciclovir / Q000633:toxicity*; D006801:Humans; D012516:Osteosarcoma / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology; D013937:Thymidine Kinase / Q000235:genetics / Q000378:metabolism* / Q000633:toxicity
chemical_list: D013937:Thymidine Kinase; C092717:thymidine kinase 1; D015774:Ganciclovir