title: Impact of wines and wine constituents on cyclooxygenase-1, cyclooxygenase-2, and 5-lipoxygenase catalytic activity.
abstract: Cyclooxygenases and lipoxygenases are proinflammatory enzymes; the former affects platelet aggregation, vasoconstriction, vasodilatation and later the development of atherosclerosis. Red wines from Georgia and central and western Europe inhibited cyclooxygenase-1 (COX-1) activity in the range of 63-94%, cyclooxygenase-2 (COX-2) activity in the range of 20-44% (tested at a concentration of 5 mL/L), and 5-lipoxygenase (5-LOX) activity in the range of 72-84% (at a concentration of 18.87 mL/L). White wines inhibited 5-LOX in the range of 41-68% at a concentration of 18.87 mL/L and did not inhibit COX-1 and COX-2. Piceatannol (IC50 = 0.76 μM) was identified as a strong inhibitor of 5-LOX followed by luteolin (IC50 = 2.25 μM), quercetin (IC50 = 3.29 μM), and myricetin (IC50 = 4.02 μM). trans-Resveratrol was identified as an inhibitor of COX-1 (IC50 = 2.27 μM) and COX-2 (IC50 = 3.40 μM). Red wine as a complex mixture is a powerful inhibitor of COX-1, COX-2, and 5-LOX, the enzymes involved in eicosanoid biosynthetic pathway.
authors: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, Rozvojova 263, 165 02 Prague 6-Lysolaje, Czech Republic ; Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamycka 129, 165 21 Prague 6-Suchdol, Czech Republic.
authors: Zsofia
authors: 0000-0001-9936-9518
authors: Z
authors: Kutil
authors: Computer-Aided Molecular Design Group, Institute of Pharmacy/Pharmaceutical Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), Innrain 80/82, 6020 Innsbruck, Austria.
authors: Veronika
authors: V
authors: Temml
authors: Institute of Horticulture, Viticulture and Oenology, Agricultural University of Georgia, David Aghmashenebeli Alley13-th Km, 0159 Tbilisi, Georgia.
authors: David
authors: D
authors: Maghradze
authors: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, Rozvojova 263, 165 02 Prague 6-Lysolaje, Czech Republic.
authors: Marie
authors: 0000-0002-1146-6354
authors: M
authors: Pribylova
authors: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, Rozvojova 263, 165 02 Prague 6-Lysolaje, Czech Republic.
authors: Marcela
authors: 0000-0002-6664-6870
authors: M
authors: Dvorakova
authors: Computer-Aided Molecular Design Group, Institute of Pharmacy/Pharmaceutical Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), Innrain 80/82, 6020 Innsbruck, Austria.
authors: Daniela
authors: 0000-0003-3300-6989
authors: D
authors: Schuster
authors: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, Rozvojova 263, 165 02 Prague 6-Lysolaje, Czech Republic.
authors: Tomas
authors: T
authors: Vanek
authors: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, Rozvojova 263, 165 02 Prague 6-Lysolaje, Czech Republic.
authors: Premysl
authors: 0000-0003-0364-2247
authors: P
authors: Landa
mesh_terms: D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D002384:Catalysis; D051545:Cyclooxygenase 1 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D011794:Quercetin / Q000494:pharmacology; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology; D014920:Wine
chemical_list: D016861:Cyclooxygenase Inhibitors; D005419:Flavonoids; D013267:Stilbenes; C040015:myricetin; D011794:Quercetin; D001094:Arachidonate 5-Lipoxygenase; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D000077185:Resveratrol