title: An anthocyanin-rich strawberry extract protects against oxidative stress damage and improves mitochondrial functionality in human dermal fibroblasts exposed to an oxidizing agent.
abstract: This study investigates the protective effect of the Sveva strawberry polyphenol-rich extract on human dermal fibroblasts against AAPH-induced oxidative stress. The HPLC-DAD/ESI-MS analysis was used for evaluating the phenolic composition of the fruits. Sveva strawberry presented a high anthocyanin content (639.79 mg per kg fresh fruit), representing ∼86.08% of the total phenolic content, with Pg-3-glc as the most abundant representative (611.18 mg per kg fresh fruit). Only one ellagitannin (agrimoniin) was identified, while two quercetins, three kaempherol derivates, and three ellagic acid derivatives were detected and quantified. Strawberry pre-treatment (0.5 mg ml(-1)) markedly increased human dermal fibroblast viability, with a significant reduction of apoptotic and dead cells, and suppressed AAPH-induced ROS generation, after only 30 minutes of incubation with the oxidizing agent, and lipid peroxidation, against a range of AAPH concentrations tested. Notably, the strawberry extract also improved the mitochondrial functionality: the basal respiratory performance after treatment was ∼1.59-fold higher compared to control cells, while pre-treatment with strawberry extract before oxidative damage increased ∼2.70-fold compared to stressed cells. Our results confirm that the strawberry possesses antioxidant properties, and may be useful for the prevention of free radical-induced skin damage.
authors: Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, Via Ranieri 65, 60131, Ancona, Italy.
authors: Francesca
authors: F
authors: Giampieri
authors: José M
authors: JM
authors: Alvarez-Suarez
authors: Luca
authors: L
authors: Mazzoni
authors: Tamara Y
authors: TY
authors: Forbes-Hernandez
authors: Massimiliano
authors: M
authors: Gasparrini
authors: Ana M
authors: AM
authors: Gonzàlez-Paramàs
authors: Celestino
authors: C
authors: Santos-Buelga
authors: Josè L
authors: JL
authors: Quiles
authors: Stefano
authors: S
authors: Bompadre
authors: Bruno
authors: B
authors: Mezzetti
authors: Maurizio
authors: M
authors: Battino
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D002460:Cell Line; D004610:Ellagic Acid / Q000032:analysis / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D031985:Fragaria / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D006801:Humans; D047348:Hydrolyzable Tannins / Q000032:analysis / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects*; D016877:Oxidants / Q000009:adverse effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology; D011794:Quercetin / Q000032:analysis / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019207:beta Carotene / Q000032:analysis / Q000494:pharmacology
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D047348:Hydrolyzable Tannins; D016877:Oxidants; D010936:Plant Extracts; D059808:Polyphenols; D020011:Protective Agents; D017382:Reactive Oxygen Species; C013515:ellagitannin; D019207:beta Carotene; D004610:Ellagic Acid; D011794:Quercetin