title: Effect of synthetic antimicrobial peptides on Naegleria fowleri trophozoites.
abstract: We evaluated the effect of tritrpticin, lactoferrin, killer decapeptide and scrambled peptide in vitro against Naegleria fowleri trophozoites compared with amphotericin B. Tritrpticin (100 microg/ml) caused apoptosis of N. fowleri trophozoites (2x10(5) cells/ml), while lactoferrin, killer decapeptide and scrambled peptide did not. On Gormori trichrome staining, tritrpticin affected the elasticity of the surface membrane and reduced the size of the nuclei of N. fowleri trophozoites. The ultrastructure surface membrane and food cup formation of the trophozoites were 100% inhibited. These results are consistent with inhibition of the nfa1, Mp2CL5 of the treated trophozoite, which plays a role in food cup formation. Tritrpticin 100 microg/ml was not toxic against SK-N-MC cells. Our findings suggest tritrpticin has activity against the surface membrane and nfa1 and Mp2CL5 of N. fowleri trophozoites and could be developed as a potential therapeutic agent.
authors: Supathra
authors: S
authors: Tiewcharoen
authors: Watchara
authors: W
authors: Phurttikul
authors: Jundee
authors: J
authors: Rabablert
authors: Prasert
authors: P
authors: Auewarakul
authors: Sittiruk
authors: S
authors: Roytrakul
authors: Pruksawan
authors: P
authors: Chetanachan
authors: Thassanant
authors: T
authors: Atithep
authors: Virach
authors: V
authors: Junnu
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000913:Antibodies, Protozoan; D017209:Apoptosis / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D018920:Coculture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D055646:Killer Factors, Yeast / Q000494:pharmacology; D007781:Lactoferrin / Q000494:pharmacology; D008565:Membrane Proteins / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D016848:Naegleria fowleri / Q000187:drug effects*; D009447:Neuroblastoma / Q000473:pathology; D009842:Oligopeptides / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins; D013194:Staining and Labeling; D053058:Trophozoites / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D000890:Anti-Infective Agents; D000913:Antibodies, Protozoan; C524619:KP killer peptide; D055646:Killer Factors, Yeast; D008565:Membrane Proteins; D009842:Oligopeptides; D010446:Peptide Fragments; D015800:Protozoan Proteins; C402895:tritrpticin; D007781:Lactoferrin