title: Polyoxygenated 24,28-epoxyergosterols inhibiting the proliferation of glioma cells from sea anemone Anthopleura midori.
abstract: Eleven sterols (1-11) and one carotenoid (12) were isolated and identified from sea anemone Anthopleura midori. Compounds 1-6 are rare polyoxygenated ergosterols with a 24,28-epoxy moiety. The structures of these epoxyergosterols were determined by NMR and HRESIMS analyses as well as their chemical-physical properties. Epoxyergosterols 1 and 2 were found to be new natural products and 3-6 are new compounds. Bioactive assay showed that compounds 1, 2, 3, 5, 7, 8, 11, and 12 inhibited the proliferation of rat glioma C6 and human glioma U251 cells with IC50 in a range of 2.41-80.45 μM. Further investigation suggested that 1 and 3 induced apoptosis in glioma cells and 1 blocked U251 cells at the G0/G1 phase.
authors: Ocean College, Zhejiang University, Hangzhou 310058, China.
authors: Siran
authors: S
authors: Yu
authors: Ocean College, Zhejiang University, Hangzhou 310058, China.
authors: Xuewei
authors: X
authors: Ye
authors: Ocean College, Zhejiang University, Hangzhou 310058, China.
authors: Lu
authors: L
authors: Chen
authors: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: xylian@zju.edu.cn.
authors: Xiao-Yuan
authors: XY
authors: Lian
authors: Ocean College, Zhejiang University, Hangzhou 310058, China. Electronic address: zzhang88@zju.edu.cn.
authors: Zhizhen
authors: Z
authors: Zhang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004875:Ergosterol / Q000737:chemistry* / Q000494:pharmacology*; D005910:Glioma / Q000473:pathology*; D006801:Humans; D010100:Oxygen / Q000737:chemistry*; D051381:Rats; D012615:Sea Anemones / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D010100:Oxygen; D004875:Ergosterol
keywords: Anthopleura midori; Epoxyergosterols; Glioma cells; Proliferation inhibition; Sea anemone