title: Real-time PCR detection of Brucella spp. DNA in lesions and viscera of bovine carcasses.
abstract: This study reports a real-time PCR assay for the detection of Brucella spp. associated with the FTA® Elute method in lesions observed during sanitary inspections in beef slaughter. Of the total 276 samples, 78 (28.3%) tested positive and 198 (71.7%) negative for Brucella spp. The real-time PCR technique associated with the FTA® Elute method proved to be an important tool for the diagnosis, judgment about and disposal of carcasses and viscera of slaughtered animals.
authors: Centro de Pesquisa em Alimentos, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: mcsmarilia@gmail.com.
authors: Marília Cristina
authors: MC
authors: Sola
authors: Centro de Pesquisa em Alimentos, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors: Eurione A G
authors: EA
authors: da Veiga Jardim
authors: Serviço de Inspeção Federal, Ministério da Agricultura, Pecuária e Abastecimento - MAPA, Goiás, Brazil.
authors: Marcius Ribeiro
authors: MR
authors: de Freitas
authors: Centro de Pesquisa em Alimentos, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors: Albenones José
authors: AJ
authors: de Mesquita
mesh_terms: D000818:Animals; D002002:Brucella / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics*; D008460:Meat / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014781:Viscera / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial
keywords: Assessment of carcasses and viscera; Brucellosis; FTA® Elute card