title: A bacteria deriving peptide modified dendrigraft poly-l-lysines (DGL) self-assembling nanoplatform for targeted gene delivery.
abstract: Achieving effective gene therapy for glioma depends on gene delivery systems. The gene delivery system should be able to cross the blood-brain barrier (BBB) and further target glioma at its early stage. Active brain tumor targeted delivery can be achieved using the "Trojan horse" technology, which involves either endogenous ligands or extraneous substances that can recognize and bind to specific receptors in target sites. This method facilitates receptor-mediated endocytosis to cross the BBB and enter into glioma cells. Dendrigraft poly-l-lysines (DGLs), which are novel nonviral gene vectors, are conjugated to a peptide (sequence: EPRNEEK) derived from Streptococcus pneumonia, a pathogen causing meningitis. This process yields peptide-modified nanoparticles (NPs) after DNA loading. Cellular uptake and in vivo imaging results indicate that EPRNEEK peptide-modified NPs have a better brain tumor targeted effect compared with a pentapeptide derived from endogenous laminin after intravenous injection. The mechanism of this effect is further explored in the present study. Besides, EPRNEEK peptide-modified NPs also exhibited a prolonged median survival time. In conclusion, the EPRNEEK peptide-modified DGL NPs exhibit potential as a nonviral platform for efficient, noninvasive, and safe brain glioma dual-targeted gene delivery.
authors: Key Laboratory of Smart Drug Delivery, Ministry of Education, School of Pharmacy, Fudan University , No. 826 Zhangheng Road, Shanghai 201203, People's Republic of China.
authors: Yang
authors: Y
authors: Liu
authors: Xi
authors: X
authors: He
authors: Yuyang
authors: Y
authors: Kuang
authors: Sai
authors: S
authors: An
authors: Chenyu
authors: C
authors: Wang
authors: Yubo
authors: Y
authors: Guo
authors: Haojun
authors: H
authors: Ma
authors: Jinning
authors: J
authors: Lou
authors: Chen
authors: C
authors: Jiang
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D006801:Humans; D008239:Lysine / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013296:Streptococcus pneumoniae / Q000737:chemistry*
chemical_list: D010455:Peptides; D008239:Lysine
keywords: brain-targeted; gene delivery; laminin receptor; nanoparticles; tumor-targeted