title: Fine physical and genetic mapping of powdery mildew resistance gene MlIW172 originating from wild emmer (Triticum dicoccoides).
abstract: Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important wheat diseases in the world. In this study, a single dominant powdery mildew resistance gene MlIW172 was identified in the IW172 wild emmer accession and mapped to the distal region of chromosome arm 7AL (bin7AL-16-0.86-0.90) via molecular marker analysis. MlIW172 was closely linked with the RFLP probe Xpsr680-derived STS marker Xmag2185 and the EST markers BE405531 and BE637476. This suggested that MlIW172 might be allelic to the Pm1 locus or a new locus closely linked to Pm1. By screening genomic BAC library of durum wheat cv. Langdon and 7AL-specific BAC library of hexaploid wheat cv. Chinese Spring, and after analyzing genome scaffolds of Triticum urartu containing the marker sequences, additional markers were developed to construct a fine genetic linkage map on the MlIW172 locus region and to delineate the resistance gene within a 0.48 cM interval. Comparative genetics analyses using ESTs and RFLP probe sequences flanking the MlIW172 region against other grass species revealed a general co-linearity in this region with the orthologous genomic regions of rice chromosome 6, Brachypodium chromosome 1, and sorghum chromosome 10. However, orthologous resistance gene-like RGA sequences were only present in wheat and Brachypodium. The BAC contigs and sequence scaffolds that we have developed provide a framework for the physical mapping and map-based cloning of MlIW172.
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Shuhong
authors: S
authors: Ouyang
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Dong
authors: D
authors: Zhang
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China; Agriculture University of Beijing, Beijing, China.
authors: Jun
authors: J
authors: Han
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Xiaojie
authors: X
authors: Zhao
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Yu
authors: Y
authors: Cui
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China; Maize Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing, China.
authors: Wei
authors: W
authors: Song
authors: USDA-ARS West Regional Research Center, Albany, California, United States of America.
authors: Naxin
authors: N
authors: Huo
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Yong
authors: Y
authors: Liang
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Jingzhong
authors: J
authors: Xie
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Zhenzhong
authors: Z
authors: Wang
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Qiuhong
authors: Q
authors: Wu
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Yong-Xing
authors: YX
authors: Chen
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Ping
authors: P
authors: Lu
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: De-Yun
authors: DY
authors: Zhang
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Lili
authors: L
authors: Wang
authors: State Key Laboratory of Plant Cell and Chromosome Engineering, Institutes of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors: Hua
authors: H
authors: Sun
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Tsomin
authors: T
authors: Yang
authors: Murdoch University, Perth, Western Australia, Australia.
authors: Gabriel
authors: G
authors: Keeble-Gagnere
authors: Murdoch University, Perth, Western Australia, Australia.
authors: Rudi
authors: R
authors: Appels
authors: Institute of Experimental Botany, Centre of Plant Structural and Functional Genomics, Olomouc, Czech Republic.
authors: Jaroslav
authors: J
authors: Doležel
authors: State Key Laboratory of Plant Cell and Chromosome Engineering, Institutes of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors: Hong-Qing
authors: HQ
authors: Ling
authors: Department of Plant Sciences, University of California, Davis, Davis, California, United States of America.
authors: Mingcheng
authors: M
authors: Luo
authors: USDA-ARS West Regional Research Center, Albany, California, United States of America.
authors: Yongqiang
authors: Y
authors: Gu
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Qixin
authors: Q
authors: Sun
authors: State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, China.
authors: Zhiyong
authors: Z
authors: Liu
mesh_terms: D001203:Ascomycota; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D011123:Polyploidy; D014908:Triticum / Q000235:genetics* / Q000382:microbiology