title: Thermodynamic evaluation of immobilized cellulose tris(3,5-dichlorophenylcarbamate) as a stationary phase for liquid chromatographic separation of darunavir enantiomers.
abstract: Liquid chromatographic separation of darunavir (DRV) enantiomers was studied on Chiralpak IC column containing immobilized cellulose tris(3,5-dichlorophenylcarbamate) using a variety of mobile phase solvents at different temperatures. The separations were accomplished under normal phase conditions using different compositions of n-hexane, organic modifier (2-propanol, ethanol or 1-propanol) and diethyl amine (0.1%) as mobile phase solvents. The effect of volume and nature of organic modifier and column temperature on retention, separation and resolution were studied. Van't Hoff plots (ln k' vs 1/T) were drawn from the chromatographic retention data to calculate to apparent thermodynamic parameters and explain the interactions between the DRV enantiomers and cellulose tris(3,5-dichlorophenylcarbamate) immobilized on silica.
authors: Analytical Chemistry Division, D 215, Discovery Laboratory, Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500 607, India rnrao55@yahoo.com rnrao@iict.res.in.
authors: R
authors: R
authors: Nageswara Rao
authors: Analytical Chemistry Division, D 215, Discovery Laboratory, Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500 607, India.
authors: K
authors: K
authors: Nagesh Kumar
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D000069454:Darunavir; D048448:Phenylcarbamates / Q000737:chemistry*; D020005:Propanols / Q000737:chemistry; D013237:Stereoisomerism; D013449:Sulfonamides / Q000737:chemistry* / Q000302:isolation & purification*; D013816:Thermodynamics
chemical_list: D048448:Phenylcarbamates; D020005:Propanols; D013449:Sulfonamides; C420325:cellulose tris(3,5-dichlorophenylcarbamate); D002482:Cellulose; D000069454:Darunavir