title: [Establishment of a hepatic fibrosis model induced by diethylnitrosamine in zebrafish].
abstract: OBJECTIVE
  To establish a zebrafish model of liver fibrosis via diethylnitrosamine (DEN)-induced liver injury.
  
  
  METHODS
  A total of 120 wild-type 3-month-old zebrafish were randomly divided into DEN-treated group and control group. The survival rate and behavioral changes of each group were observed. After treatment with DEN for 2, 4, and 6 weeks, liver index was measured, and liver fibrosis was evaluated with HE staining, Gomori staining and Sirius red staining.
  
  
  RESULTS
  No obvious behavioral change was observed in DEN-treated group during the experiment. Compared with that in control group, the liver index of zebrafish in DEN-treated group showed no significantly changes at the time points of observation. Proliferation of reticulate fibers was found in 30% of zebrafish treated with DEN for 4 weeks, and the rate increased to 80% at 6 weeks when reticulate fibers and collagen fibers actively proliferated to result in fiber collapse and formation of fibrotic nodules.
  
  
  CONCLUSIONS
  A stable zebrafish liver fibrosis model was successfully established by inducing liver damage to facilitate studies of the pathogenesis of liver fibrosis and screening therapeutic drugs.
authors: Department of Infectious Diseases and Hepatology Unit, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: wangkunyuan@sina.cn.
authors: Kunyuan
authors: K
authors: Wang
authors: Li
authors: L
authors: Liu
authors: Wencong
authors: W
authors: Dai
authors: Xiaohui
authors: X
authors: Chen
authors: Xinchun
authors: X
authors: Zheng
authors: Jinlin
authors: J
authors: Hou
mesh_terms: D000818:Animals; D004052:Diethylnitrosamine; D004195:Disease Models, Animal; D008103:Liver Cirrhosis / Q000139:chemically induced*; D015027:Zebrafish
chemical_list: D004052:Diethylnitrosamine