title: Dynamics of T-cell IFN-γ and miR-29a expression during active pulmonary tuberculosis.
abstract: IFN-γ is crucial for protection against Mycobacterium tuberculosis. miR-29 was recently shown to non-redundantly inhibit IFN-γ. Here, we investigated IFN-γ and miR-29a expression dynamics of CD4(+) T cells from patients during active tuberculosis (TB) (n = 32) and in household contacts who were latently M. tuberculosis infected (n = 19) from Ghana. Whereas M. tuberculosis-specific IFN-γ expression was similar during TB chemotherapy, superantigen stimulation indicated generally impaired IFN-γ expression in TB patients. No interdependency between miR-29a and IFN-γ expression of T cells was observed. However, miR-29a was differentially expressed in T cells during chemotherapy. We concluded that differential miR-29a expression in active TB was not causative for impaired IFN-γ expression.
authors: Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana School of Medical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
authors: Anthony
authors: A
authors: Afum-Adjei Awuah
authors: Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, Duesseldorf 40225, Germany.
authors: Bianca
authors: B
authors: Ueberberg
authors: Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana School of Medical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
authors: Ellis
authors: E
authors: Owusu-Dabo
authors: School of Medical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
authors: Margaret
authors: M
authors: Frempong
authors: Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, Duesseldorf 40225, Germany Department of Immunology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg 20359, Germany marc.jacobsen@med.uni-duesseldorf.de.
authors: Marc
authors: M
authors: Jacobsen
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D060085:Coinfection; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011446:Prospective Studies; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D014397:Tuberculosis, Pulmonary / Q000235:genetics* / Q000276:immunology*; D055815:Young Adult
chemical_list: C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D007371:Interferon-gamma
keywords: IFN-γ; T cell; TB; miR-29a