title: Tumour angiogenesis and repulsive guidance molecule b: a role in HGF- and BMP-7-mediated angiogenesis.
abstract: Hepatocyte growth factor (HGF) is a key growth factor linked to promoting cancer progression and angiogenesis. The present study identifies repulsive guidance molecule b (RGMb), a bone morphogenetic protein (BMP) co-receptor, as a gene whose expression is regulated by HGF and explores the potential of RGMb to contribute to the process of angiogenesis. Microarray analysis was used to identify HGF responsive genes in HECV endothelial cells, identifying RGMb. RGMb was subsequently targeted using a ribozyme transgene system and its role in angiogenesis assessed using in vitro and in vivo assays. The importance of RGMb in pro-angiogenic responses to HGF and BMP-7 was also assessed. Microarray analysis identified RGMb as a gene upregulated as a result of HGF treatment. Knockdown of RGMb, in HECV cells, had minimal effects on tubule formation, brought about a general, although non-significant increase in cell growth and enhanced cell migration. Similarly, no significant effect of RGMb knockdown was found in vivo using a co-inoculation angiogenesis model. Knockdown of RGMb was, however, found to reduce the responsiveness of HECV cells to HGF treatment and particularly to BMP-7 treatment in regard to the enhanced migratory and tubule formation brought about by these treatments in vitro. Our results indicate that RGMb expression can be influenced by HGF treatment. Whilst this molecule appears to have minimal impact on angiogenic traits individually, it demonstrates an involvement in propagating pro-angiogenic effects of HGF and particularly BMP-7 and thus, may play a role in regulating angiogenic responses to HGF and BMP-7.
authors: Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, UK.
authors: Andrew J
authors: AJ
authors: Sanders
authors: Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, UK.
authors: Lin
authors: L
authors: Ye
authors: Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, UK.
authors: Jin
authors: J
authors: Li
authors: Section of Oncology and Palliative Medicine, Cardiff University School of Medicine, Cardiff, UK.
authors: Malcolm D
authors: MD
authors: Mason
authors: Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, UK.
authors: Wen G
authors: WG
authors: Jiang
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000378:metabolism*; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D042783:Endothelial Cells / Q000378:metabolism*; D058851:GPI-Linked Proteins; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017228:Hepatocyte Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000473:pathology*; D009374:Neoplasms, Experimental; D009389:Neovascularization, Pathologic / Q000378:metabolism; D018919:Neovascularization, Physiologic; D009419:Nerve Tissue Proteins / Q000378:metabolism
chemical_list: D055419:Bone Morphogenetic Protein 7; D015816:Cell Adhesion Molecules, Neuronal; D058851:GPI-Linked Proteins; D009419:Nerve Tissue Proteins; C560433:RGMB protein, human; C483497:Rgmb protein, mouse; D017228:Hepatocyte Growth Factor