title: Multi-modal molecular diffuse optical tomography system for small animal imaging.
abstract: A multi-modal optical imaging system for quantitative 3D bioluminescence and functional diffuse imaging is presented, which has no moving parts and uses mirrors to provide multi-view tomographic data for image reconstruction. It is demonstrated that through the use of trans-illuminated spectral near infrared measurements and spectrally constrained tomographic reconstruction, recovered concentrations of absorbing agents can be used as prior knowledge for bioluminescence imaging within the visible spectrum. Additionally, the first use of a recently developed multi-view optical surface capture technique is shown and its application to model-based image reconstruction and free-space light modelling is demonstrated. The benefits of model-based tomographic image recovery as compared to 2D planar imaging are highlighted in a number of scenarios where the internal luminescence source is not visible or is confounding in 2D images. The results presented show that the luminescence tomographic imaging method produces 3D reconstructions of individual light sources within a mouse-sized solid phantom that are accurately localised to within 1.5mm for a range of target locations and depths indicating sensitivity and accurate imaging throughout the phantom volume. Additionally the total reconstructed luminescence source intensity is consistent to within 15% which is a dramatic improvement upon standard bioluminescence imaging. Finally, results from a heterogeneous phantom with an absorbing anomaly are presented demonstrating the use and benefits of a multi-view, spectrally constrained coupled imaging system that provides accurate 3D luminescence images.
authors: Physical Science of Imaging in the Biomedical Sciences Doctoral Training Centre, College of Engineering and Physical Sciences, University of Birmingham, UK ; School of Computer Science, College of Engineering and Physical Sciences, University of Birmingham, UK.
authors: James A
authors: JA
authors: Guggenheim
authors: Physical Science of Imaging in the Biomedical Sciences Doctoral Training Centre, College of Engineering and Physical Sciences, University of Birmingham, UK ; School of Computer Science, College of Engineering and Physical Sciences, University of Birmingham, UK.
authors: Hector R A
authors: HR
authors: Basevi
authors: School of Immunity and Infection, College of Medicine and Dentistry, University of Birmingham, UK.
authors: Jon
authors: J
authors: Frampton
authors: School of Computer Science, College of Engineering and Physical Sciences, University of Birmingham, UK.
authors: Iain B
authors: IB
authors: Styles
authors: Physical Science of Imaging in the Biomedical Sciences Doctoral Training Centre, College of Engineering and Physical Sciences, University of Birmingham, UK ; School of Computer Science, College of Engineering and Physical Sciences, University of Birmingham, UK.
authors: Hamid
authors: H
authors: Dehghani
keywords: Bioluminescence Imaging; Bioluminescence Tomography; Diffuse Optical Tomography; Image Reconstruction; Imaging Systems; Molecular Imaging; Multimodality; Small Animal Imaging; Surface Capture