title: Proteomic analysis of Populus × euramericana (clone I-214) roots to identify key factors involved in zinc stress response.
abstract: Contamination of soil and water by heavy metals has become a widespread problem; environmental pollution by high zinc (Zn) concentration occurs frequently. Although poplar (Populus spp.) has been identified as suitable for phytoremediation approaches, its response to high Zn concentrations are still not clearly understood. For this reason, we investigated the effects of Zn in Populus×euramericana clone I-214 roots by proteomic analysis. Comparative experiments were conducted on rooted woody cuttings grown in nutrient solutions containing 1mM (treatment) or 1μM (control) Zn concentrations. A gel-based proteomic approach coupled with morphological and chemical analysis was used to identify differentially represented proteins in treated roots and to investigate the effect of Zn treatment on the poplar root system. Data shows that Zn was accumulated preferentially in roots, that the antioxidant system, the carbohydrate/energy and amino acid metabolisms were the main pathways modulated by Zn excess, and that mitochondria and vacuoles were the cellular organelles predominately affected by Zn stress. A coordination between cell death and proliferation/growth seems to occur under this condition to counteract the Zn-induced damage.
authors: BioLabs, Institute of Life Sciences, Scuola Superiore Sant'Anna, 56127 Pisa, Italy.
authors: Stefania
authors: S
authors: Romeo
authors: Dipartimento di Bioscienze e Territorio, University of Molise, 86090 Pesche, IS, Italy.
authors: Dalila
authors: D
authors: Trupiano
authors: BioLabs, Institute of Life Sciences, Scuola Superiore Sant'Anna, 56127 Pisa, Italy.
authors: Andrea
authors: A
authors: Ariani
authors: Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Napoli, Italy.
authors: Giovanni
authors: G
authors: Renzone
authors: Dipartimento di Bioscienze e Territorio, University of Molise, 86090 Pesche, IS, Italy.
authors: Gabriella S
authors: GS
authors: Scippa
authors: Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Napoli, Italy.
authors: Andrea
authors: A
authors: Scaloni
authors: BioLabs, Institute of Life Sciences, Scuola Superiore Sant'Anna, 56127 Pisa, Italy. Electronic address: luca.sebastiani@sssup.it.
authors: Luca
authors: L
authors: Sebastiani
mesh_terms: D003433:Crosses, Genetic; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism / Q000187:drug effects; D016166:Free Radical Scavengers / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D018547:Plant Stems / Q000187:drug effects / Q000378:metabolism; D032107:Populus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013312:Stress, Physiological / Q000187:drug effects*; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism; D015032:Zinc / Q000633:toxicity*
chemical_list: D016166:Free Radical Scavengers; D010940:Plant Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D015032:Zinc
keywords: Mitochondrial homeostasis; Poplar; Root proliferation; Vacuolar dysfunction; Zn