title: Biocompatible mesoporous silica-coated superparamagnetic manganese ferrite nanoparticles for targeted drug delivery and MR imaging applications.
abstract: Multifunctional mesoporous silica-coated superparamagnetic manganese ferrite (MnFe2O4) nanoparticles (M-MSN) were synthesized and evaluated for targeted drug delivery and magnetic resonance imaging (MRI) applications. MnFe2O4 nanoparticles were prepared by solvothermal route and were silica-coated by surface silylation using sol-gel reactions. Subsequently, silylation was done using (3-aminopropyl)triethoxysilane in presence of a surfactant (CTAB), followed by selective etching of the surfactant molecules that resulted in amine-functionalized superparamagnetic nanoparticles (NH2-MSN). Further modification of the surface of the NH2-MSN with targeting (folate) or fluorescent (RITC) molecules resulted in M-MSN. The formation of the M-MSN was proved by several characterization techniques viz. XRD, XPS, HRTEM, FESEM, VSM, BET surface area measurement, FTIR, and UV-Vis spectroscopy. The M-MSN were loaded with anticancer drug Doxorubicin and the efficacy of the DOX loaded M-MSN was evaluated through in vitro cytotoxicity, fluorescence microscopy, and apoptosis studies. The in vivo biocompatibility of the M-MSN was demonstrated in a mice-model system. Moreover, the M-MSN also acted as superior MRI contrast agent owing to a high magnetization value as well as superparamagnetic behavior at room temperature. These folate-conjugated nanoparticles (FA-MSN) exhibited stronger T2-weighted MRI contrast towards HeLa cells as compared to the nanoparticles without folate conjugation, justifying their potential importance in MRI based diagnosis of cancer. Such M-MSN with a magnetic core required for MRI imaging, a porous shell for carrying drug molecules, a targeting moeity for cancer cell specificity and a fluorescent molecule for imaging, all integrated into a single system, may potentially serve as an excellent material in biomedical applications.
authors: Department of Chemistry, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors: Banalata
authors: B
authors: Sahoo
authors: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors: K Sanjana P
authors: KS
authors: Devi
authors: Department of Chemistry, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors: Sujan
authors: S
authors: Dutta
authors: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors: Tapas K
authors: TK
authors: Maiti
authors: Department of Chemistry, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors: Panchanan
authors: P
authors: Pramanik
authors: Department of Chemistry, Indian Institute of Technology Kharagpur, West Bengal 721302, India. Electronic address: dibakar@chem.iitkgp.ernet.in.
authors: Dibakar
authors: D
authors: Dhara
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D017895:Manganese Compounds / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000903:Antibiotics, Antineoplastic; D020099:Coated Materials, Biocompatible; D005290:Ferric Compounds; D017895:Manganese Compounds; C551151:manganese ferrite; D012822:Silicon Dioxide; D004317:Doxorubicin
keywords: Cancer specificity; Doxorubicin; Fluorescence imaging; Folic acid; Mesoporous silica; Multifunctional nanoparticles