title: A novel fluorescent "turn-on" chemosensor for nanomolar detection of Fe(III) from aqueous solution and its application in living cells imaging.
abstract: An electronically active and spectral sensitive fluorescent "turn-on" chemosensor (BTP-1) based on the benzo-thiazolo-pyrimidine unit was designed and synthesized for the highly selective and sensitive detection of Fe(3+) from aqueous medium. With Fe(3+), the sensor BTP-1 showed a remarkable fluorescence enhancement at 554 nm (λex = 314 nm) due to the inhibition of photo-induced electron transfer. The sensor formed a host-guest complex in 1:1 stoichiometry with the detection limit down to 0.74 nM. Further, the sensor was successfully utilized for the qualitative and quantitative intracellular detection of Fe(3+) in two liver cell lines i.e., HepG2 cells (human hepatocellular liver carcinoma cell line) and HL-7701 cells (human normal liver cell line) by a confocal imaging technique.
authors: School of Chemical Sciences, North Maharashtra University, P. B. No. 80, Jalgaon 425001, MS, India.
authors: Jitendra
authors: J
authors: Nandre
authors: School of Chemical Sciences, North Maharashtra University, P. B. No. 80, Jalgaon 425001, MS, India.
authors: Samadhan
authors: S
authors: Patil
authors: School of Chemical Sciences, North Maharashtra University, P. B. No. 80, Jalgaon 425001, MS, India.
authors: Vijay
authors: V
authors: Patil
authors: Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
authors: Fabiao
authors: F
authors: Yu
authors: Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. Electronic address: lxchen@yic.ac.cn.
authors: Lingxin
authors: L
authors: Chen
authors: Department of Applied Chemistry, S.V. National Institute Technology, Surat 395007, Gujrat, India.
authors: Suban
authors: S
authors: Sahoo
authors: Department of Chemistry, University of Hull, Cottingham Road, Hull HU6 7RX, UK.
authors: Timothy
authors: T
authors: Prior
authors: Department of Chemistry, University of Hull, Cottingham Road, Hull HU6 7RX, UK.
authors: Carl
authors: C
authors: Redshaw
authors: School of Chemical Sciences, North Maharashtra University, P. B. No. 80, Jalgaon 425001, MS, India. Electronic address: mahulikarpp@rediffmail.com.
authors: Pramod
authors: P
authors: Mahulikar
authors: School of Chemical Sciences, North Maharashtra University, P. B. No. 80, Jalgaon 425001, MS, India. Electronic address: udpatil.nmu@gmail.com.
authors: Umesh
authors: U
authors: Patil
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D005290:Ferric Compounds / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D007501:Iron / Q000032:analysis*; D057230:Limit of Detection; D008099:Liver / Q000166:cytology*; D008856:Microscopy, Fluorescence / Q000379:methods; D061848:Optical Imaging / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D005456:Fluorescent Dyes; D013844:Thiazoles; C039201:thiazolo(3,2-a)perimidine; D007501:Iron
keywords: Benzo-thiazolo-pyrimidine; Fe(3+); Fluorescent “turn-on” sensor; ICT; Live cells imaging.; PET