title: Effect of chronic coffee consumption on weight gain and glycaemia in a mouse model of obesity and type 2 diabetes.
abstract: OBJECTIVE
  Epidemiological evidence shows that chronic coffee consumption in humans is correlated with a lower incidence of type 2 diabetes mellitus. For the experimental exploration of the underlying mechanisms, this effect needs to be replicated in an animal model of type 2 diabetes with a short lifespan.
  
  
  METHODS
  Male C57BL/6 mice consumed regular coffee or water ad libitum and the development of obesity and diabetes caused by high-fat diet (55% lipids, HFD) was observed from week 10 on for 35 weeks in comparison with mice feeding on a defined normal diet (9% lipids, ND).
  
  
  RESULTS
  The massive weight gain in HFD mice was dose-dependently retarded (P=0.034), the moderate weight gain in ND mice was abolished (P<0.001) by coffee consumption, probably because of a lower feeding efficiency. The consumption of fluid (water or coffee) was significantly diminished by HFD (P<0.001), resulting in a higher coffee exposure of ND mice. On week 21 intraperitoneal glucose tolerance tests (IPGTT) showed a dose-dependent faster decline of elevated glucose levels in coffee-consuming HFD mice (P=0.016), but not in ND mice. Remarkably, a spontaneous decrease in non-fasting glycaemia occurred after week 21 in all treatment groups (P<0.001). On week 39 the IPGTT showed diminished peak of glucose levels in coffee-consuming HFD mice (P<0.05). HFD mice were hyperinsulinaemic and had significantly (P<0.001) enlarged islets. Coffee consumption did not affect islet size or parameters of beta-cell apoptosis, proliferation and insulin granule content.
  
  
  CONCLUSIONS
  Coffee consumption retarded weight gain and improved glucose tolerance in a mouse model of type 2 diabetes and corresponding controls. This gives rise to the expectation that further insight into the mechanism of the diabetes-preventive effect of coffee consumption in humans may be gained by this approach.
authors: Institute of Pharmacology and Toxicology, University of Braunschweig, Braunschweig, Germany.
authors: I
authors: I
authors: Rustenbeck
authors: Institute of Pharmacology and Toxicology, University of Braunschweig, Braunschweig, Germany.
authors: V
authors: V
authors: Lier-Glaubitz
authors: Institute of Pharmacology and Toxicology, University of Braunschweig, Braunschweig, Germany.
authors: M
authors: M
authors: Willenborg
authors: Institute of Psychology, University of Braunschweig, Braunschweig, Germany.
authors: F
authors: F
authors: Eggert
authors: Institute of Food Chemistry, University of Braunschweig, Braunschweig, Germany.
authors: U
authors: U
authors: Engelhardt
authors: Department of Anatomy, Hannover Medical School, Hannover, Germany.
authors: A
authors: A
authors: Jörns