title: Measurement of mitochondrial membrane potential in leukocyte suspension by fluorescent spectroscopy.
abstract: A method for evaluating mitochondrial membrane potential in isolated leukocyte suspension with the use of sensitive fl uorescent cation-active dye 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine (JC-1) and spectrophotometry is described. JC-1 monomer rapidly penetrates through mitochondrial membrane of living cell and form aggregations characterized by red fl uorescence (λ = 585 nm). In case of mitochondrial membrane depolarization (early sign of apoptosis), JC-1 is not accumulated in the mitochondria and is present in the cytoplasm as a monomer characterized by green spectral fl uorescence (λ = 510 nm). The method can be used for evaluation of the function of living cells and mechanisms regulating energy metabolism by evaluating the mitochondrial membrane potential.
authors: Research Institute of Medical Climatology and Rehabilitative Treatment, Vladivostok Affiliated Department of Far-Eastern Research Center of Respiration Physiology and Pathology, Siberian Division of the Russian Academy of Medical Sciences, Vladivostok, Russia.
authors: E G
authors: EG
authors: Lobanova
authors: E V
authors: EV
authors: Kondrat'eva
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000502:physiology*; D008875:Middle Aged; D013050:Spectrometry, Fluorescence / Q000379:methods*