title: Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
abstract: Chromatin regulatory proteins affect diverse developmental and environmental response pathways via their influence on nuclear processes such as the regulation of gene expression. Through a genome-wide genetic screen, we implicate a novel protein called X-chromosome-associated protein 5 (Xap5) in chromatin regulation. We show that Xap5 is a chromatin-associated protein acting in a similar manner as the histone variant H2A.Z to suppress expression of antisense and repeat element transcripts throughout the fission yeast genome. Xap5 is highly conserved across eukaryotes, and a plant homolog rescues xap5 mutant yeast. We propose that Xap5 likely functions as a chromatin regulator in diverse organisms.
authors: Department of Plant Biology, College of Biological Sciences University of California, Davis, CA, USA.
authors: Shajahan
authors: S
authors: Anver
authors: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA, USA.
authors: Assen
authors: A
authors: Roguev
authors: Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.
authors: Martin
authors: M
authors: Zofall
authors: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA, USA.
authors: Nevan J
authors: NJ
authors: Krogan
authors: Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.
authors: Shiv I S
authors: SI
authors: Grewal
authors: Department of Plant Biology, College of Biological Sciences University of California, Davis, CA, USA slharmer@ucdavis.edu.
authors: Stacey L
authors: SL
authors: Harmer
mesh_terms: D016375:Antisense Elements (Genetics); D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006657:Histones / Q000502:physiology*; D011485:Protein Binding; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000502:physiology*; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D016375:Antisense Elements (Genetics); D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D006657:Histones; C091242:PHT1 protein, S pombe; D012331:RNA, Fungal; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins; C000596329:Xap5 protein, S pombe
keywords: aberrant transcripts; chromatin modification; long terminal repeats; transcriptional repression; transposable elements