title: Inactivation of Aggregatibacter actinomycetemcomitans by two different modalities of photodynamic therapy using Toluidine blue O or Radachlorin as photosensitizers: an in vitro study.
abstract: Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) is one of the periodontopathogens strongly associated with aggressive periodontitis. The aim of this investigation was to compare the effect of laser and light-emitting diode on the photodynamic inactivation of A. actinomycetemcomitans. Eighty-four samples of bacterial suspensions (200 μL) were prepared and divided in seven groups: control group (no treatment), laser group (indium-gallium-aluminum-phosphate laser with wavelength of 662 ± 0.1 nm, energy density of 6 j/cm(2), and irradiation time of 34 s), light-emitting diode (LED) group (wavelength 625-635 nm, energy density 6 j/cm(2), time of irradiation 30 s), Toluidine blue O (TBO) group (0.1 mg/mL), Radachlorin group (0.1 %), Radachlorin + laser group (after pre-irradiation time of 10 min, laser was irradiated), and TBO + LED group (after preirradiation time of 10 min, LED was irradiated). Then, 100 μL of each sample was cultured in brain heart infusion (BHI) plates and incubated for 48-72 h in microaerophilic atmosphere for colony counting. Application of Radachlorin + laser resulted in a significant decrease in the concentration of A. actinomycetemcomitans (P values <0.05). Photodynamic therapy with laser + Radachlorin was more effective than that of LED + TBO in suppression of this microorganism (P value <0.05). Within the limits of this study, it can be concluded that photodynamic inactivation using laser and Radachlorin was more effective than that of LED and TBO in eradication of A. actinomycetemcomitans.
authors: Laser Research Center of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors: Neda
authors: N
authors: Moslemi
authors: Pardis
authors: P
authors: Soleiman-Zadeh Azar
authors: Abbas
authors: A
authors: Bahador
authors: Nina
authors: N
authors: Rouzmeh
authors: Nasim
authors: N
authors: Chiniforush
authors: Mojgan
authors: M
authors: Paknejad
authors: Reza
authors: R
authors: Fekrazad
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects / Q000502:physiology*; D004338:Drug Combinations; D006801:Humans; D053844:Lasers, Solid-State; D008826:Microbial Sensitivity Tests; D016871:Pasteurellaceae Infections / Q000188:drug therapy; D010518:Periodontitis / Q000188:drug therapy / Q000382:microbiology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000494:pharmacology*; D014048:Tolonium Chloride / Q000494:pharmacology*
chemical_list: D004338:Drug Combinations; D017319:Photosensitizing Agents; D011166:Porphyrins; C000593241:bremachlorin; D014048:Tolonium Chloride