title: Sensitivities, specificities, and predictive values of microbiological culture techniques for the diagnosis of prosthetic joint infection.
abstract: BACKGROUND
  Identifying the microorganism in a prosthetic joint infection is the key to appropriately targeting antimicrobial treatment. Despite the availability of various techniques, no single test is considered the definitive gold standard.
  
  
  OBJECTIVE
  Our aim was to determine the sensitivity, specificity, and positive/negative predictive values for a variety of culture techniques.
  
  
  METHODS
  We performed a retrospective case series of 219 patients undergoing revision surgery of their hip or knee replacement between May 2004 and February 2013. The patients were classified as either infected or noninfected according to criteria set out by the Musculoskeletal Infection Society. The number and type of samples taken intraoperatively varied between cases but included tissue samples and fluid sent in either blood culture vials or sterile containers.
  
  
  RESULTS
  The highest sensitivity was found with blood culture vials (0.85) compared to fluid in sterile containers (0.26) and tissues samples (0.32). Blood culture vials also had a better specificity and positive and negative predictive values profile.
  
  
  CONCLUSIONS
  We conclude that, of the techniques studied, fluid in blood culture vials had the best profile for the correct identification of microorganisms and advocate its use.
authors: University Hospital Coventry & Warwickshire, Clifford Bridge Road, Coventry CV2 2DX, UK.
authors: Robert W
authors: RW
authors: Jordan
authors: University of Warwick, Coventry CV4 7AL, UK.
authors: Nicholas A
authors: NA
authors: Smith
authors: University Hospital Coventry & Warwickshire, Clifford Bridge Road, Coventry CV2 2DX, UK.
authors: Adnan
authors: 0000-0002-2408-5307
authors: A
authors: Saithna
authors: University of Warwick, Coventry CV4 7AL, UK.
authors: Andrew P
authors: AP
authors: Sprowson
authors: University Hospital Coventry & Warwickshire, Clifford Bridge Road, Coventry CV2 2DX, UK.
authors: Pedro
authors: 0000-0003-3858-1050
authors: P
authors: Foguet
mesh_terms: D001424:Bacterial Infections / Q000097:blood* / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology*; D001431:Bacteriological Techniques / Q000379:methods; D005260:Female; D006801:Humans; D007595:Joint Prosthesis / Q000382:microbiology*; D008297:Male; D012189:Retrospective Studies