title: Relationship between interleukin-1 type 1 and 2 receptor gene polymorphisms and the expression level of membrane-bound receptors.
abstract: The biological activity of the multifunctional cytokine interleukin-1 (IL-1) is mediated by its receptors. The aim of this study was to determine if an association exists between single nucleotide polymorphisms (SNPs) in the IL-1 type 1 and 2 receptor genes (IL1R1 and IL1R2) and the expression level of membrane-bound IL1Rs on subpopulations of mononuclear cells or serum levels of soluble IL-1 receptors. It was observed that healthy individuals with the genotype TT in SNP rs2234650:C>T had a lower percentage of intact CD14(+) monocytes expressing IL1R1 on their surface. The SNP rs4141134:T>C in IL1R2 has also been associated with the percentage of intact CD3(+) T cells expressing IL1R2. Furthermore, individuals carrying the CC allele of SNP rs4141134:T>C and the TT allele of SNP rs2071008:T>G in IL1R2 had a lower density of IL1R2s on the surface of CD14(+) monocytes in lipopolysaccharide (LPS)-stimulated PBMC cultures. In summary, this study demonstrated that IL-1 receptor gene polymorphisms could be one of the factors influencing the expression of membrane-bound IL-1 receptors (IL1R) on immunocompetent cells.
authors: F F
authors: FF
authors: Vasilyev
authors: A N
authors: AN
authors: Silkov
authors: S V
authors: SV
authors: Sennikov
mesh_terms: D000328:Adult; D002462:Cell Membrane / Q000378:metabolism*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D053573:Receptors, Interleukin-1 Type I / Q000097:blood* / Q000235:genetics*; D053574:Receptors, Interleukin-1 Type II / Q000097:blood* / Q000235:genetics*; D055815:Young Adult
chemical_list: C508590:IL1R1 protein, human; C508592:IL1R2 protein, human; D008070:Lipopolysaccharides; D053573:Receptors, Interleukin-1 Type I; D053574:Receptors, Interleukin-1 Type II