title: Changes in the bacterial community of soybean rhizospheres during growth in the field.
abstract: Highly diverse communities of bacteria inhabiting soybean rhizospheres play pivotal roles in plant growth and crop production; however, little is known about the changes that occur in these communities during growth. We used both culture-dependent physiological profiling and culture independent DNA-based approaches to characterize the bacterial communities of the soybean rhizosphere during growth in the field. The physiological properties of the bacterial communities were analyzed by a community-level substrate utilization assay with BioLog Eco plates, and the composition of the communities was assessed by gene pyrosequencing. Higher metabolic capabilities were found in rhizosphere soil than in bulk soil during all stages of the BioLog assay. Pyrosequencing analysis revealed that differences between the bacterial communities of rhizosphere and bulk soils at the phylum level; i.e., Proteobacteria were increased, while Acidobacteria and Firmicutes were decreased in rhizosphere soil during growth. Analysis of operational taxonomic units showed that the bacterial communities of the rhizosphere changed significantly during growth, with a higher abundance of potential plant growth promoting rhizobacteria, including Bacillus, Bradyrhizobium, and Rhizobium, in a stage-specific manner. These findings demonstrated that rhizosphere bacterial communities were changed during soybean growth in the field.
authors: Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto, Japan.
authors: Akifumi
authors: A
authors: Sugiyama
authors: Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto, Japan.
authors: Yoshikatsu
authors: Y
authors: Ueda
authors: Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto, Japan.
authors: Takahiro
authors: T
authors: Zushi
authors: Faculty of Bioenvironmental Science, Kyoto Gakuen University, Kameoka, Kyoto, Japan.
authors: Hisabumi
authors: H
authors: Takase
authors: Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto, Japan.
authors: Kazufumi
authors: K
authors: Yazaki
mesh_terms: D064307:Microbiota; D010802:Phylogeny; D025341:Principal Component Analysis; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013025:Glycine max / Q000254:growth & development* / Q000382:microbiology*