title: Association of vitamin E with rapid thawing on goat semen.
abstract: The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws and cooled at 5°C for 1 hour. Freezing was performed in liquid nitrogen vapor (-155°C) during 15 minutes. Then, the straws were immersed in liquid nitrogen (-196°C). Straws were thawed at 38°C/60 seconds or at 60°C/7 seconds with immediate sperm analysis. Hypoosmotic swelling test was performed adding a 20 μL aliquot of thawed semen to 1 mL of hypoosmotic solution (100 mOsm · Kg(-1)) followed by incubation during 60 minutes in water bath (38°C). Vitamin E did not affect any studied parameters (P > 0.05). Nevertheless, defrosting rate of 60°C/7 seconds improved sperm membrane functional integrity (P < 0.05). Current knowledge about goat semen cryopreservation is not sufficient to ensure high post-thawing recovery rates; thus, this study brings important data about using antioxidants and different thawing rates on cryopreservation process.
authors: Department of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil ; Laboratory of Physiology and Animal Reproduction, Department of Animal Science, Universidade Federal de Viçosa, P. H. Rolfs Avenue, 36570-900 Viçosa, MG, Brazil.
authors: Jurandy Mauro
authors: JM
authors: Penitente-Filho
authors: Department of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil.
authors: Fabrício Albani
authors: FA
authors: Oliveira
authors: Department of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil.
authors: Carolina Rodriguez
authors: CR
authors: Jimenez
authors: Department of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil.
authors: Erly
authors: 0000-0002-4947-4093
authors: E
authors: Carrascal
authors: Department of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil.
authors: Júlio César Oliveira
authors: JC
authors: Dias
authors: Department of Veterinary, Federal University of Viçosa, P. H. Rolfs Avenue, 36570-900 Viçosa, MG, Brazil.
authors: Gisele Dias
authors: GD
authors: Oliveira
authors: Federal University of Espírito Santo, University Campus, 29500-000 Alegre, ES, Brazil.
authors: Renata Gomes
authors: RG
authors: Silveira
authors: Department of Veterinary, Federal University of Viçosa, P. H. Rolfs Avenue, 36570-900 Viçosa, MG, Brazil.
authors: Camila Oliveira
authors: CO
authors: Silveira
authors: Department of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil.
authors: Ciro Alexandre Alves
authors: CA
authors: Torres
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D006041:Goats; D008297:Male; D012661:Semen / Q000737:chemistry*; D014810:Vitamin E / Q000737:chemistry*
chemical_list: D014810:Vitamin E