title: Cord blood transplantation is associated with rapid B-cell neogenesis compared with BM transplantation.
abstract: Hematopoietic cell transplantation (HCT) is used for treatment of hematopoietic diseases. Assessment of T- and B-cell reconstitution after HCT is crucial because poor immune recovery has a major effect on the clinical course. In this study, we retrospectively analyzed T-cell receptor excision circles (TRECs) as well as signal and coding joint kappa-deleting recombination excision circles (sjKRECs and cjKRECs, respectively) as markers of newly produced lymphocytes in 133 patients (56 primary immunodeficient and 77 malignant cases, median (range): 12 (0-62) years old). We analyzed the kinetics of TREC and KREC recovery and determined the factors that contributed to better immune recovery. KRECs became positive earlier than TRECs and increased thereafter. Younger recipient age had a favorable effect on recovery of sjKRECs and cjKRECs. Compared with BM and peripheral blood, our data suggested that cord blood (CB) provided rapid B-cell recovery. CB also provided better B-cell neogenesis in adult HCT recipients. Chronic GVHD was associated with low TRECs, but not increased sjKRECs/cjKRECs. Finally, positive sjKRECs 1 month after HCT were associated with fewer infectious episodes. Monitoring of TRECs and KRECs may serve as a useful tool for assessment of immune reconstitution post HCT.
authors: Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, Tokyo, Japan.
authors: K
authors: K
authors: Nakatani
authors: Department of Pediatrics, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: K
authors: K
authors: Imai
authors: Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, Tokyo, Japan.
authors: M
authors: M
authors: Shigeno
authors: Department of Preventive Medicine and Public Health, National Defense Medical College, Saitama, Japan.
authors: H
authors: H
authors: Sato
authors: Department of Pediatrics, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: M
authors: M
authors: Tezuka
authors: Department of Pediatrics, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: T
authors: T
authors: Okawa
authors: Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, Tokyo, Japan.
authors: N
authors: N
authors: Mitsuiki
authors: Department of Pediatrics, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: T
authors: T
authors: Isoda
authors: Department of Pediatrics, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: D
authors: D
authors: Tomizawa
authors: Department of Pediatrics, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: M
authors: M
authors: Takagi
authors: Department of Pediatrics, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: M
authors: M
authors: Nagasawa
authors: Department of Blood Transfusion, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: M
authors: M
authors: Kajiwara
authors: Department of Hematology, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: M
authors: M
authors: Yamamoto
authors: Department of Hematology, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: A
authors: A
authors: Arai
authors: Department of Hematology, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: O
authors: O
authors: Miura
authors: Department of Pediatrics, National Defense Medical College, Saitama, Japan.
authors: C
authors: C
authors: Kamae
authors: Department of Pediatrics, National Defense Medical College, Saitama, Japan.
authors: N
authors: N
authors: Nakagawa
authors: Department of Pediatrics, National Defense Medical College, Saitama, Japan.
authors: K
authors: K
authors: Honma
authors: Department of Pediatrics, National Defense Medical College, Saitama, Japan.
authors: S
authors: S
authors: Nonoyama
authors: 1] Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, Tokyo, Japan [2] Department of Pediatrics, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: S
authors: S
authors: Mizutani
authors: 1] Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, Tokyo, Japan [2] Department of Pediatrics, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
authors: T
authors: T
authors: Morio
mesh_terms: D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005312:Fetal Blood / Q000637:transplantation*; D006402:Hematologic Diseases / Q000276:immunology / Q000628:therapy*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000097:blood / Q000276:immunology; D012189:Retrospective Studies; D019172:Transplantation Conditioning / Q000379:methods; D055815:Young Adult
chemical_list: D011948:Receptors, Antigen, T-Cell