title: Photocrosslinked ultrathin anionic polysaccharide supports for accelerated growth of human mesenchymal stem cells.
abstract: OBJECTIVE
  Properties of cell culture supports obtained from ultrathin multilayer films containing anionic natural polysaccharides (PSacs) and a synthetic polycation were studied.
  
  
  METHODS
  Supports were prepared via a layer-by-layer (LbL) self-assembly deposition method. Polymers used were: heparin (Hep), chondroitin sulphate (CS), hyaluronic acid (HA), and ι-carrageenan (Car) as polyanions, and diazoresin (DR) as a polycation. PSac layers were crosslinked with DR layers by irradiation with UV light absorbed by DR resin.
  
  
  RESULTS
  DR/PSac films are very efficient cell culture growth supports as found from experiments with human mesenchymal stem cells (hMSCs). Irradiation of the films resulted in changing zeta potential of outermost layers of both DR and PSac to more negative values, and in increased film hydrophobicity, as found from the contact angle measurements. Photocrosslinking of the supports led to their increased stability.
  
  
  CONCLUSIONS
  The supports allow for obtaining intact cell monolayers faster than when typical polystyrene tissue culture plates are used. Moreover, these monolayers spontaneously detach permitting formation of new cell layers on these surfaces relatively early during culture, compared to cells cultured on commonly used tissue culture plastic.
authors: Faculty of Chemistry, Jagiellonian University, 30-387, Kraków, Poland.
authors: A
authors: A
authors: Mikulska
authors: J
authors: J
authors: Filipowska
authors: A M
authors: AM
authors: Osyczka
authors: M
authors: M
authors: Szuwarzyński
authors: M
authors: M
authors: Nowakowska
authors: K
authors: K
authors: Szczubiałka
mesh_terms: D001391:Azo Compounds / Q000138:chemical synthesis / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D058428:Silica Gel / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D001391:Azo Compounds; D001672:Biocompatible Materials; D000071228:Polyelectrolytes; D011108:Polymers; D011134:Polysaccharides; D058428:Silica Gel; C504851:diazoresin