title: Differential transcriptome analysis of the common shrimp Crangon crangon: special focus on the nuclear receptors and RNAi-related genes.
abstract: The decapod Crangon crangon is one of the most valuable European fisheries commodities. Despite its economic importance, little sequence data is available for this shrimp species. In this paper, we report the transcriptome sequencing for five different stages of C. crangon (early embryo, late embryo, larva, female adults and male adults) and the annotation and stage-specific expression analysis of nuclear receptors (NRs) and RNA interference (RNAi)-related genes. The NRs are transcription factors that play an essential role in growth, development, cell differentiation, molting/metamorphosis and reproduction, while the RNAi-related genes are very important for internal gene expression regulation and in antiviral defense. We discovered a NR in the female C. crangon which is either a very rapidly evolved homolog of HR10, or a novel NR altogether. This new NR could act as a biological marker for sex determination as it is not expressed in male adults. Most RNAi-related genes were present in C. crangon, proving that the requirements for successful RNAi is present in this decapod shrimp. RNAi-based applications in Crangon such as its use in functional genomics or as antiviral therapeutics could become very important in the near future.
authors: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
authors: Olivier
authors: O
authors: Christiaens
authors: Institute for Agricultural and Fisheries Research (ILVO), Animal Sciences Unit, Fisheries, Ankerstraat 1, 8400 Ostend, Belgium.
authors: Daan
authors: D
authors: Delbare
authors: Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium.
authors: Christophe
authors: C
authors: Van Neste
authors: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
authors: Kaat
authors: K
authors: Cappelle
authors: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
authors: Na
authors: N
authors: Yu
authors: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium; Institute for Agricultural and Fisheries Research (ILVO), Animal Sciences Unit, Fisheries, Ankerstraat 1, 8400 Ostend, Belgium.
authors: Ruben
authors: R
authors: De Wilde
authors: Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium.
authors: Filip
authors: F
authors: Van Nieuwerburgh
authors: Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium.
authors: Dieter
authors: D
authors: Deforce
authors: Institute for Agricultural and Fisheries Research (ILVO), Animal Sciences Unit, Fisheries, Ankerstraat 1, 8400 Ostend, Belgium.
authors: Kris
authors: K
authors: Cooreman
authors: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium. Electronic address: guy.smagghe@ugent.be.
authors: Guy
authors: G
authors: Smagghe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D033501:Crangonidae / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D034622:RNA Interference / Q000502:physiology*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D018160:Receptors, Cytoplasmic and Nuclear
keywords: Crangon crangon; Crustacea; Decapoda; Nuclear receptors; RNAi-related genes; Transcriptomics