title: Near-infrared dye bound albumin with separated imaging and therapy wavelength channels for imaging-guided photothermal therapy.
abstract: Development of theranostic agent for imaging-guided photothermal therapy has been of great interest in the field of nanomedicine. However, if fluorescent imaging and photothermal ablation are conducted with the same wavelength of light, the requirements of the agent's quantum yield (QY) for imaging and therapy are controversial. In this work, our synthesized near-infrared dye, IR825, is bound with human serum albumin (HSA), forming a HSA-IR825 complex with greatly enhanced fluorescence under 600 nm excitation by as much as 100 folds compared to that of free IR825, together with a rather high absorbance but low fluorescence QY at 808 nm. Since high QY that is required for fluorescence imaging would result in reduced photothermal conversion efficiency, the unique optical behavior of HSA-IR825 enables imaging and photothermal therapy at separated wavelengths both with optimized performances. We thus use HSA-IR825 for imaging-guided photothermal therapy in an animal tumor model. As revealed by in vivo fluorescence imaging, HSA-IR825 upon intravenous injection shows high tumor uptake likely owing to the enhanced permeability and retention effect, together with low levels of retentions in other organs. While HSA is an abundant protein in human serum, IR825 is able to be excreted by renal excretion as evidenced by high-performance liquid chromatography (HPLC). In vivo tumor treatment experiment is finally carried out with HSA-IR825, achieving 100% of tumor ablation in mice using a rather low dose of IR825. Our work presents a safe, simple, yet imageable photothermal nanoprobe, promising for future clinical translation in cancer treatment.
authors: Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu 215123, China.
authors: Qian
authors: Q
authors: Chen
authors: Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu 215123, China.
authors: Chao
authors: C
authors: Wang
authors: Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu 215123, China.
authors: Zhixiong
authors: Z
authors: Zhan
authors: Department of Polymer Science and Engineering, Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors: Weiwei
authors: W
authors: He
authors: Department of Polymer Science and Engineering, Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors: Zhenping
authors: Z
authors: Cheng
authors: Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu 215123, China. Electronic address: yyli@suda.edu.cn.
authors: Youyong
authors: Y
authors: Li
authors: Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu 215123, China. Electronic address: zliu@suda.edu.cn.
authors: Zhuang
authors: Z
authors: Liu
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000818:Animals; D001565:Benzoates / Q000737:chemistry*; D002417:Cattle; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005021:Ethylamines / Q000737:chemistry; D005260:Female; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D007211:Indoles / Q000737:chemistry*; D007668:Kidney / Q000187:drug effects; D007834:Lasers; D008027:Light; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000628:therapy*; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D019265:Spectroscopy, Near-Infrared
chemical_list: D000418:Albumins; D001565:Benzoates; D004396:Coloring Agents; D005021:Ethylamines; C000601126:IR825 dye; D007211:Indoles; D012709:Serum Albumin; C016162:triethylamine
keywords: Fluorescent imaging; Human serum albumin; NIR dye; Photothermal therapy; Tumor metastasis