title: [Effects of ceruloplasmin/PTEN on histone modifications induced by silica].
abstract: OBJECTIVE
  To explore the roles of ceruloplasmin (Cp) in histone modifications in human embryonic lung fibroblasts (HELFs) induced silica and the effects of phosphatase and tension homolog deleted on chromosome ten (PTEN) in this process.
  
  
  METHODS
  HELFs were exposed to different concentrations of silica (50, 100, 200 microg/ ml) or Cp (10, 20, 30 microg/ml) for 24 h, and the level of protein lysine acetylation, of histone H2, H3 and H4 acetylation and histone H3 methylation were checked by werstern blot assay. HELFs and cells transfected with PTEN shRNA (PT) were treated with 200 microg/ml silica for 1 h, then added 30 microg/ml Cp for 24 h. Acetylation levels of protein lysine, histone H2, H3, H4 and methylation level of histone H3 were detected by werstern blot assay.
  
  
  RESULTS
  Silica could induce the high level of protein lysine acetylation and acetyl-histone H2B (lys5/12), acetyl-histone H3 (lys9/14), acetyl-histone H4 (lys12) and the low level of methyl-histone (arg2), which could be reversed by Cp, in no exception for acetyl-histone H2B (lys5/12). Cp couldn't reverse histone modifications induced by silica when inhibited PTEN.
  
  
  CONCLUSIONS
  Cp could reverse silica-induced the change of histone acetylation and histone methylation, and PTEN involved in this process.
authors: Xia'nan
authors: X
authors: Zhang
authors: Yuegang
authors: Y
authors: Li
authors: Xiaowei
authors: X
authors: Jia
authors: Bingci
authors: B
authors: Liu
authors: Meng
authors: M
authors: Ye
mesh_terms: D000107:Acetylation; D002570:Ceruloplasmin; D005347:Fibroblasts; D006657:Histones; D006801:Humans; D008239:Lysine; D008745:Methylation; D010084:Oxidation-Reduction; D051059:PTEN Phosphohydrolase; D011506:Proteins; D012822:Silicon Dioxide / Q000494:pharmacology*
chemical_list: D006657:Histones; D011506:Proteins; D012822:Silicon Dioxide; D002570:Ceruloplasmin; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D008239:Lysine