title: [Identification of Peucedani Radix and its adulterants by DNA barcoding technique].
abstract: OBJECTIVE
  To identify Peucedani Radix and its adulterants using DNA barcoding technique.
  
  
  METHODS
  Total genomic DNA was isolated from Peucedani Radix and its adulterants. Nuclear DNA ITS2 sequences were amplified and purified PCR products were sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner V3.0. The Kimura 2-Parameter(K2P) distances were calculated using software MEGA 4. 0. Identification analyses were performed using BLAST1, Nearest Distance and Neighbor-Joining (NJ) methods, and the secondary structure of the ITS2 sequence differences between species were analyzed.
  
  
  RESULTS
  Different samples of Peucedani Radix were gathered together and distinguished from its adulterants by NJ tree. The ITS2 secondary structure showed that Peucedani Radix could be differentiated obviously from its adulterants.
  
  
  CONCLUSIONS
  ITS2 sequence is able to identify Peucedani Radix and its adulterants correctly, which provides a scientific basis for fast and accurate identification of the herb.
authors: Yong-xing
authors: YX
authors: Xiong
authors: Lan
authors: L
authors: Wu
authors: Yi-mei
authors: YM
authors: Liu
authors: Ke-li
authors: KL
authors: Chen
authors: Yong-xing
authors: YX
authors: Xiong
authors: Lan
authors: L
authors: Wu
authors: Yi-mei
authors: YM
authors: Liu
authors: Ke-li
authors: KL
authors: Chen
mesh_terms: D019661:Apiaceae / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D058893:DNA Barcoding, Taxonomic; D018744:DNA, Plant / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D004340:Drug Contamination; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction; D011786:Quality Control; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer