title: Site-directed RNA editing with antagomir deaminases--a tool to study protein and RNA function.
abstract: RNA-guided machineries perfectly satisfy the demand for rationally programmable tools that manipulate gene function inside the cell. Over the last ten years, various natural machineries have been harnessed, with RNA interference being among the most prominent examples. It is now time to tackle the engineering of novel RNA-guided tools not provided by nature. In this respect, we highlight RNA-guided site-directed RNA editing as a new concept for the manipulation of RNA and protein function. In contrast to currently available techniques, RNA editing allows for the introduction of selected point mutations into the transcriptome without the need for genomic manipulation. In particular, the approach described using chemically stabilized, antagomir-like guideRNAs may offer advantages over others, such as specificity and circumvention of immunogenicity. These new tools have significant potential for the advancement of both basic science and medicinal application, especially in the treatment of genetic diseases.
authors: Interfaculty Institute of Biochemistry, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen (Germany) http://www.ifib.uni-tuebingen.de/research/stafforst.html.
authors: Paul
authors: P
authors: Vogel
authors: Thorsten
authors: T
authors: Stafforst
mesh_terms: D000818:Animals; D006801:Humans; D016297:Mutagenesis, Site-Directed; D009700:Nucleoside Deaminases / Q000737:chemistry* / Q000494:pharmacology; D015202:Protein Engineering; D011506:Proteins / Q000187:drug effects* / Q000502:physiology; D012313:RNA / Q000187:drug effects* / Q000502:physiology; D017393:RNA Editing / Q000187:drug effects*
chemical_list: D011506:Proteins; D012313:RNA; D009700:Nucleoside Deaminases
keywords: RNA; bioengineering; editing; genetic diseases; nucleic acid analogues