title: Dual role of host Par2 in a murine model of spontaneous metastatic B16 melanoma.
abstract: OBJECTIVE
  We investigated differences of metastatic spread of normal proteinase-activated receptor-2 (Par2+/+) melanoma B16 in Par2-/- (knock-out) animals compared to C57Bl6 mice.
  
  
  METHODS
  Nine knock-out mice B6.Cg-F2rl1tm1Mslb/J (Par2-/-) and nine C57Bl6/J controls were subcutaneously inoculated with B16 melanoma tissue cells. Twelve days after inoculation, all primary tumors were removed. Survival and metastatic spread was followed for up to 100 days after primary tumor extirpation.
  
  
  RESULTS
  Excised primary tumors were on average larger in Par2-/- mice (360 mm3 vs. 221 mm3 in C57Bl6/J). Distant spontaneous metastases developed in only 3 of 9 of Par2-/- mice in comparison to 6 of 9 controls. The average survival time was 84 days in Par2-/- animals compared to 63 days in C57Bl6/J mice.
  
  
  CONCLUSIONS
  Host Par2 melanoma model contributes to the limitation of local cancer progression in one area, while on the other hand is important for enhancing distant metastatic spread.
authors: Department of Pathology and Molecular Medicine, Thomayer Hospital, Prague, Czech Republic Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Tomas
authors: T
authors: Olejar
authors: Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic CancerTech s.r.o., Pruhonek, Prague, Czech Republic.
authors: David
authors: D
authors: Vetvicka
authors: Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors: Marie
authors: M
authors: Zadinova
authors: Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors: Pavla
authors: P
authors: Pouckova
authors: Department of Software Engineering, Faculty of Nuclear Science and Physical Engineering, Czech Technical University, Prague, Czech Republic.
authors: Jaromir
authors: J
authors: Kukal
authors: Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Petr
authors: P
authors: Jezek
authors: Department of Pathology and Molecular Medicine, Thomayer Hospital, Prague, Czech Republic Department of Pathology, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic radoslav.matej@ftn.cz.
authors: Radoslav
authors: R
authors: Matej
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D008297:Male; D008546:Melanoma, Experimental / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D044464:Receptor, PAR-2 / Q000235:genetics* / Q000378:metabolism
chemical_list: D044464:Receptor, PAR-2
keywords: PAR2; melanoma; metastasis; murine model