title: Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism.
abstract: Although type 1 cannabinoid receptors (CB1Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors (CB2Rs) in neurons is still somewhat controversial. Taking advantage of newly designed CB1R and CB2R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of CB1Rs and CB2Rs, as well as CB1R-CB2R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of CB1Rs, CB2Rs and CB1R-CB2R heteromers was found in dyskinetic animals, mimicking the observed reduction in CB1R and CB2R mRNA expression levels. The fact that chronic levodopa treatment disrupted CB1R-CB2R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers.
authors: Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain.
authors: Salvador
authors: S
authors: Sierra
authors: Natasha
authors: N
authors: Luquin
authors: Alberto J
authors: AJ
authors: Rico
authors: Virginia
authors: V
authors: Gómez-Bautista
authors: Elvira
authors: E
authors: Roda
authors: Iria G
authors: IG
authors: Dopeso-Reyes
authors: Alfonso
authors: A
authors: Vázquez
authors: Eva
authors: E
authors: Martínez-Pinilla
authors: José L
authors: JL
authors: Labandeira-García
authors: Rafael
authors: R
authors: Franco
authors: José L
authors: JL
authors: Lanciego
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism*; D002186:Cannabinoids / Q000378:metabolism; D007980:Levodopa / Q000378:metabolism; D008251:Macaca; D008297:Male; D009474:Neurons / Q000378:metabolism*; D020734:Parkinsonian Disorders; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism*; D043885:Receptor, Cannabinoid, CB2 / Q000378:metabolism*
chemical_list: D002186:Cannabinoids; C571431:Cnr2 protein, mouse; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2; D007980:Levodopa