title: Expression patterns of miR-221 and its target Caspase-3 in different cancer cell lines.
abstract: Caspases are important initiators and most well-known finishers of apoptosis. By changing the death propagation homeostatic equilibrium, their different expression patterns might trigger the progression of hazardous diseases like cancer. miR-221 is an oncogenic miRNA. It is known to have both anti-angiogenic and angiogenic effect. The aim of this work was to compare the expression levels of miR-221 and its target caspase-3 in different cancer cell lines and to find out a relationship between these two. We also tried to establish a prominent relationship between miR-221 and its role in apoptosis by studying their expression levels. Our results indicate that expression of caspase-3 is quite lower as compared to miR-221 expression in all of the selected cancer cell lines. As a result, we conclude that miR-221 may have a crucial role in repressing the expression of caspase-3 which may contribute to a lower apoptotic rate, thus supporting the selection of more aggressive cancer cells. To our knowledge, this is the first study related to the expression levels of caspase-3 and miR-221 in different cell lines at the same time. We expect that our study might pave the way for better understanding the role of miR-221 in apoptotic regulation of caspase-3.
authors: Department of Medical Biology, Faculty of Medicine, Gaziantep University, Şehitkamil, 27310, Gaziantep, Turkey, sercanergun@msn.com.
authors: Sercan
authors: S
authors: Ergun
authors: Kaifee
authors: K
authors: Arman
authors: Ebru
authors: E
authors: Temiz
authors: Ibrahim
authors: I
authors: Bozgeyik
authors: Önder
authors: Ö
authors: Yumrutaş
authors: Muhammad
authors: M
authors: Safdar
authors: Hasan
authors: H
authors: Dağlı
authors: Ahmet
authors: A
authors: Arslan
authors: Serdar
authors: S
authors: Oztuzcu
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D053148:Caspase 3; D035683:MicroRNAs; C525709:MIR221, human; C505407:CASP3 protein, human