title: Obesity favors apolipoprotein E- and C-III-containing high density lipoprotein subfractions associated with risk of heart disease.
abstract: Human HDLs have highly heterogeneous composition. Plasma concentrations of HDL with apoC-III and of apoE in HDL predict higher incidence of coronary heart disease (CHD). The concentrations of HDL-apoA-I containing apoE, apoC-III, or both and their distribution across HDL sizes are unknown. We studied 20 normal weight and 20 obese subjects matched by age, gender, and race. Plasma HDL was separated by sequential immunoaffinity chromatography (anti-apoA-I, anti-apoC-III, anti-apoE), followed by nondenaturing-gel electrophoresis. Mean HDL-cholesterol concentrations in normal weight and obese subjects were 65 and 50 mg/dl (P = 0.009), and total apoA-I concentrations were 119 and 118 mg/dl, respectively. HDL without apoE or apoC-III was the most prevalent HDL type representing 89% of apoA-I concentration in normal weight and 77% in obese (P = 0.01) individuals; HDL with apoE-only was 5% versus 8% (P = 0.1); HDL with apoC-III-only was 4% versus 10% (P = 0.009); and HDL with apoE and apoC-III was 1.5% versus 4.6% (P = 0.004). Concentrations of apoE and apoC-III in HDL were 1.5-2× higher in obese subjects (P ≤ 0.004). HDL with apoE or apoC-III occurred in all sizes among groups. Obese subjects had higher prevalence of HDL containing apoE or apoC-III, subfractions associated with CHD, whereas normal weight subjects had higher prevalence of HDL without apoE or apoC-III, subfractions with protective association against CHD.
authors: Department of Nutrition, Harvard School of Public Health, Boston, MA.
authors: Beatriz
authors: B
authors: Talayero
authors: Department of Nutrition, Harvard School of Public Health, Boston, MA.
authors: Liyun
authors: L
authors: Wang
authors: Department of Nutrition, Harvard School of Public Health, Boston, MA.
authors: Jeremy
authors: J
authors: Furtado
authors: Channing Division of Network Medicine, Boston, MA.
authors: Vincent J
authors: VJ
authors: Carey
authors: Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA.
authors: George A
authors: GA
authors: Bray
authors: Department of Nutrition, Harvard School of Public Health, Boston, MA.
authors: Frank M
authors: FM
authors: Sacks
mesh_terms: D000328:Adult; D000368:Aged; D053305:Apolipoprotein C-III / Q000097:blood*; D001057:Apolipoproteins E / Q000097:blood*; D005260:Female; D006331:Heart Diseases / Q000097:blood*; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood*
chemical_list: D053305:Apolipoprotein C-III; D001057:Apolipoproteins E; D008075:Lipoproteins, HDL
keywords: apolipoprotein A-I; coronary heart disease; high density lipoprotein size