title: Polymorphism of Interleukin-6 is not associated with the presence or absence of high HPV E6/E7.
abstract: The present study evaluated the frequency of the polymorphism of Interleukin-6 (IL6) in women positive for E6/E7 Human Papillomavirus (HPV) (n=152) and women negative for HPV (n=238), 390 women in total. Material for analysis was obtained at the Federal University of São Paulo. Interleukin-6 polymorphism was detected by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and analyzed in 3% agarose gel.
  
  
  RESULTS
  No significant associations between the frequency of the polymorphism of IL6 in patients expressing E6 and E7 with HPV-positive and -negative reactions were found. There was no statistically significant difference between the case and control group for genotype distribution (p=0.280).
  
  
  CONCLUSIONS
  Genotypic analysis showed a striking similarity of IL6 polymorphisms in both cases and controls. The allelic distribution in cases and controls for G and C of IL6 were very similar (p=0.186), which could point to similar IL6 functionality for both groups.
authors: Molecular Gynecology Laboratory, Gynecology Department, Federal University of São Paulo, São Paulo, Brazil crcinti@hotmail.com.
authors: Claudia Regina Cinti
authors: CR
authors: Porto
authors: Molecular Gynecology Laboratory, Gynecology Department, Federal University of São Paulo, São Paulo, Brazil.
authors: João Paulo Ferreira
authors: JP
authors: De Oliveira Kleine
authors: Molecular Oncology Research Center, Barretos Cancer Hospital, Pio XII Foundation, Barretos, Brazil Laboratory of Medical Investigation 14, Faculty of Medicine, University of São Paulo, São Paulo, Brazil Life and Health Sciences Research Institute, School of Health Sciences, University of Minho, Braga, Portugal Government Associate Laboratory, Braga Guimarães, Portugal.
authors: Adhemar
authors: A
authors: Longatto Filho
authors: Molecular Gynecology Laboratory, Gynecology Department, Federal University of São Paulo, São Paulo, Brazil Molecular Oncology Research Center, Barretos Cancer Hospital, Pio XII Foundation, Barretos, Brazil.
authors: Ismael Dale Cotrim Guerreiro
authors: ID
authors: Da Silva
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000821:virology; D005260:Female; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis*; D027383:Papillomaviridae / Q000378:metabolism; D030361:Papillomavirus Infections / Q000235:genetics* / Q000821:virology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic
chemical_list: D015850:Interleukin-6; D009856:Oncogene Proteins, Viral
keywords: E6/E7; Gene polymorphism; HPV; IL6