title: Enrichment of anodic biofilm inoculated with anaerobic or aerobic sludge in single chambered air-cathode microbial fuel cells.
abstract: Aerobic sludge after anaerobic pretreatment and anaerobic sludge were separately used as inoculum to start up air-cathode single-chamber MFCs. Aerobic sludge-inoculated MFCs arrived at 0.27 V with a maximum power density of 5.79 W m(-3), while anaerobic sludge-inoculated MFCs reached 0.21 V with 3.66 W m(-3). Microbial analysis with DGGE profiling and high-throughput sequencing indicated that aerobic sludge contained more diverse bacterial populations than anaerobic sludge. Nitrospira species dominated in aerobic sludge, while anaerobic sludge was dominated by Desulfurella and Acidithiobacillus species. Microbial community structure and composition in anodic biofilms enriched, respectively from aerobic and anaerobic sludges tended gradually to be similar. Potentially exoelectrogenic Geobacter and Anaeromusa species, biofilm-forming Zoogloea and Acinetobacter species were abundant in both anodic biofilms. This study indicated that aerobic sludge performed better for MFCs startup, and the enrichment of anodic microbial consortium with different inocula but same substrate resulted in uniformity of functional microbial communities.
authors: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors: Chongyang
authors: C
authors: Gao
authors: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: waj0578@hit.edu.cn.
authors: Aijie
authors: A
authors: Wang
authors: Department of Civil and Environmental Engineering, Codiga Resource Recovery Center, Center for Sustainable Development & Global Competitiveness, Stanford University, Stanford, CA 94305-4020, USA.
authors: Wei-Min
authors: WM
authors: Wu
authors: Key Laboratory of Marine Environment and Ecology (Ocean University of China), Ministry of Education, Qingdao 266100, China.
authors: Yalin
authors: Y
authors: Yin
authors: Key Laboratory of Marine Environment and Ecology (Ocean University of China), Ministry of Education, Qingdao 266100, China.
authors: Yang-Guo
authors: YG
authors: Zhao
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001419:Bacteria / Q000235:genetics / Q000254:growth & development; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D018441:Biofilms; D004275:DNA, Ribosomal / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D004560:Electricity; D004566:Electrodes; D008969:Molecular Sequence Data; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology*; D013997:Time Factors
chemical_list: D004275:DNA, Ribosomal; D012722:Sewage
keywords: Anodic microbial community; Inoculated sludge; Microbial fuel cell; Startup