title: Proteome changes induced by laser in diabetic retinopathy.
abstract: BACKGROUND
  Diabetic macular oedema (DMO) is the commonest cause of vision loss in people with diabetes. Laser photocoagulation can be effective in the treatment of DMO; however, its mechanism of action is still poorly understood. A better understanding of these mechanisms may allow the development of therapeutic approaches that could avoid the deleterious adverse events associated with photocoagulation.
  
  
  METHODS
  We have used proteomic techniques to identify the protein changes induced by threshold intensity retinal laser treatment in a rodent model of diabetic retinopathy. Retinae were obtained from diabetic Dark Agouti rats 8 weeks following laser treatment. Extracted proteins from lasered and non-lasered diabetic retinae were separated and compared using two-dimensional gel electrophoresis.
  
  
  RESULTS
  Image analysis revealed 24 protein spots with decreased expression after laser treatment and 9 spots with increased expression. On lasered retinal gels, four spots were uniquely expressed, with eight unique spots on non-lasered gels. Twenty-two protein spots of interest were identified using matrix-assisted desorption ionization-mass spectrometry with database matching. Following laser, Wnt-5 beta, LEK-1, GADPH, claudin-12 and calretinin were significantly down-regulated in expression.
  
  
  CONCLUSIONS
  The present study provides a proteomic insight into the underlying biological basis for the therapeutic effects of laser for DMO. We provide further evidence of the involvement of Wnt pathway signalling in the neural retina in DMO, and for up to 2 months following laser treatment. Changes in LEK-1 and claudin-12 may have effector roles, and changes in glyceraldehyde-3-phosphate dehydrogenase and calretinin may reflect the altered retinal microenvironment resulting from laser treatment.
authors: Save Sight Institute, Discipline of Clinical Ophthalmology, University of Sydney, Sydney, New South Wales, Australia; Australian School of Advanced Medicine, Macquarie University, Sydney, New South Wales, Australia.
authors: Godfrey J
authors: GJ
authors: Quin
authors: Brian
authors: B
authors: Lyons
authors: Alice C L
authors: AC
authors: Len
authors: Michele C
authors: MC
authors: Madigan
authors: Mark C
authors: MC
authors: Gillies
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000601:surgery*; D003930:Diabetic Retinopathy / Q000378:metabolism / Q000601:surgery*; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000378:metabolism*; D017075:Laser Coagulation / Q000379:methods*; D008297:Male; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D060449:Wnt Signaling Pathway / Q000502:physiology*
chemical_list: D005136:Eye Proteins; D020543:Proteome
keywords: Wnt-signalling pathway; diabetic macular oedema; diabetic retinopathy; laser photocoagulation