title: Involvement of upregulated SYF2 in Schwann cell differentiation and migration after sciatic nerve crush.
abstract: SYF2 is a putative homolog of human p29 in Saccharomyces cerevisiae. It seems to be involved in pre-mRNA splicing and cell cycle progression. Disruption of SYF2 leads to reduced α-tubulin expression and delayed nerve system development in zebrafish. Due to the potential of SYF2 in modulating microtubule dynamics in nervous system, we investigated the spatiotemporal expression of SYF2 in a rat sciatic nerve crush (SNC) model. We found that SNC resulted in a significant upregulation of SYF2 from 3 days to 1 week and subsequently returned to the normal level at 4 weeks. At its peak expression, SYF2 distributed predominantly in Schwann cells. In addition, upregulation of SYF2 was approximately in parallel with Oct-6, and numerous Schwann cells expressing SYF2 were Oct-6 positive. In vitro, we observed enhanced expression of SYF2 during the process of cyclic adenosine monophosphate (cAMP)-induced Schwann cell differentiation. SYF2-specific siRNA-transfected Schwann cells did not show significant morphological change in the process of Schwann cell differentiation. Also, we found shorter and disorganized microtubule structure and a decreased migration in SYF2-specific siRNA-transfected Schwann cells. Together, these findings indicated that the upregulation of SYF2 was associated with Schwann cell differentiation and migration following sciatic nerve crush.
authors: Department of Orthopaedics, Affiliated Jiangyin Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors: Zhengming
authors: Z
authors: Zhou
authors: Yang
authors: Y
authors: Liu
authors: Xiaoke
authors: X
authors: Nie
authors: Jianhua
authors: J
authors: Cao
authors: Xiaojian
authors: X
authors: Zhu
authors: Li
authors: L
authors: Yao
authors: Weidong
authors: W
authors: Zhang
authors: Jiang
authors: J
authors: Yu
authors: Gang
authors: G
authors: Wu
authors: Yonghua
authors: Y
authors: Liu
authors: Huiguang
authors: H
authors: Yang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D039481:CD11b Antigen / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D008954:Models, Biological; D009409:Nerve Crush; D009687:Nuclear Proteins / Q000378:metabolism*; D050816:Octamer Transcription Factor-6 / Q000378:metabolism; D010641:Phenotype; D034741:RNA, Small Interfering / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D012584:Sciatic Nerve / Q000473:pathology*; D014404:Tubulin / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D015415:Biomarkers; D039481:CD11b Antigen; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; C000598030:SYF2 protein, rat; D014404:Tubulin; D050816:Octamer Transcription Factor-6; D000242:Cyclic AMP