title: Characterization of a single cell of Chlorella in a microfluidic channel using amperometric electrode arrays.
abstract: Electrochemical characteristics of O2 and/or mediators secreted by a single cell of Chlorella fusea were analyzed by using amperometric measurements on microelectrodes embedded in a microfluidic device. A single cell was trapped in a microfluidic channel, which simplifies the mass transfer phenomenon, i.e., one-dimensional distribution of solutes in the channel. Such amperometric measurements allowed us to obtain more refined data in a localized space and to understand photosynthetic behavior of algae at the single cell level. In addition, the concentration of a photosynthetic mediator, p-benzoquinone, was numerically calculated by using the finite element method.
authors: Department of Fiber System Engineering, Dankook University, 152 Jukjeon-ro, Suji-gu, Yongin-si, Gyeonggi-do, South Korea.
authors: Young Seok
authors: YS
authors: Song
authors: Seoung Jai
authors: SJ
authors: Bai
mesh_terms: D016227:Benzoquinones / Q000032:analysis; D001709:Biotechnology / Q000295:instrumentation*; D002708:Chlorella / Q000737:chemistry / Q000166:cytology*; D003198:Computer Simulation; D004867:Equipment Design; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010100:Oxygen / Q000032:analysis; D010788:Photosynthesis; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods
chemical_list: D016227:Benzoquinones; C004532:quinone; D010100:Oxygen