title: Rationales and approaches for studying metabolism in eukaryotic microalgae.
abstract: The generation of efficient production strains is essential for the use of eukaryotic microalgae for biofuel production. Systems biology approaches including metabolite profiling on promising microalgal strains, will provide a better understanding of their metabolic networks, which is crucial for metabolic engineering efforts. Chlamydomonas reinhardtii represents a suited model system for this purpose. We give an overview to genetically amenable microalgal strains with the potential for biofuel production and provide a critical review of currently used protocols for metabolite profiling on Chlamydomonas. We provide our own experimental data to underpin the validity of the conclusions drawn.
authors: Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, D-14476 Potsdam-Golm, Germany. veyel@mpimp-golm.mpg.de.
authors: Daniel
authors: D
authors: Veyel
authors: Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, D-14476 Potsdam-Golm, Germany. erban@mpimp-golm.mpg.de.
authors: Alexander
authors: A
authors: Erban
authors: Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, D-14476 Potsdam-Golm, Germany. fehrle@mpimp-golm.mpg.de.
authors: Ines
authors: I
authors: Fehrle
authors: Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, D-14476 Potsdam-Golm, Germany. kopka@mpimp-golm.mpg.de.
authors: Joachim
authors: J
authors: Kopka
authors: Molecular Biotechnology & Systems Biology, Technical University of Kaiserslautern, Paul-Ehrlich-Str. 23, D-67663 Kaiserslautern, Germany. Schroda@bio.uni-kl.de.
authors: Michael
authors: M
authors: Schroda