title: Analysis of nucleic acid-induced nonimmune cell death in vitro.
abstract: Foreign nucleic acids are recognized by germ-line-encoded receptors expressed in immune and nonimmune cells. Activation of the nucleic acid-specific pattern recognition receptors by foreign nucleic acid promotes production of inflammatory cytokines (mostly type I IFNs) and at the later stage leads to cell death. Here, we describe reliable and simple methods to quantify cell death caused by nucleic acid recognition. Additionally, we report two different methods to discriminate between two cell death modalities: apoptosis and necrosis.
authors: Nephrologisches Zentrum, Medizinische Klinik und Poliklinik IV, Klinikum der Universität München, Ludwig-Maximilians-University Munich, Schillerstr. 42, 80336, Munich, Germany.
authors: Simone
authors: S
authors: Romoli
authors: Adriana
authors: A
authors: Migliorini
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D066298:In Vitro Techniques; D051379:Mice; D009696:Nucleic Acids / Q000494:pharmacology*; D051192:Receptors, Pattern Recognition / Q000378:metabolism
chemical_list: D009696:Nucleic Acids; D051192:Receptors, Pattern Recognition