title: Direct disk diffusion susceptibility testing from respiratory tract specimens: focus on Pseudomonas aeruginosa.
abstract: The aim of this study was to evaluate the application of direct disk diffusion (DDD) testing to respiratory tract specimens for the prediction of the antimicrobial susceptibility profile. DDD was performed on 144 specimens containing P. aeruginosa and the disk diffusion test was used as reference method. Agreement with the reference method was 77.8% for amikacin, 69.4% for cefepime, 86.1% for levofloxacin, 87.5% for meropenem, and 62.5% for piperacillin/tazobactam. Very major errors were observed for all agents, except levofloxacin. Our study showed that DDD results are inaccurate and may lead to errors in early decision-making regarding antibiotic therapy for lower respiratory tract infections.
authors: Hospital Mãe de Deus, Porto Alegre, Brazil; Centro de Pesquisa Experimental, Laboratório de Pesquisa em Resistência Bacteriana, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Santa Cecília, Porto Alegre, RS 90035-903, Brazil. Electronic address: leandro.reus@gmail.com.
authors: Leandro Reus Rodrigues
authors: LR
authors: Perez
authors: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Brazil.
authors: Ana Lúcia Peixoto de
authors: AL
authors: Freitas
authors: Centro de Pesquisa Experimental, Laboratório de Pesquisa em Resistência Bacteriana, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Santa Cecília, Porto Alegre, RS 90035-903, Brazil; Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Brazil.
authors: Afonso Luís
authors: AL
authors: Barth
authors: Hospital Mãe de Deus, Porto Alegre, Brazil; Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, Brazil.
authors: Cícero Armídio Gomes
authors: CA
authors: Dias
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012137:Respiratory System / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Direct susceptibility testing; Disk diffusion; Pseudomonas aeruginosa; Ventilator-associated pneumonia