title: Leucine-rich repeat-containing G-protein-coupled Receptor 5 marks short-term hematopoietic stem and progenitor cells during mouse embryonic development.
abstract: Lgr5 is a marker for proliferating stem cells in adult intestine, stomach, and hair follicle. However, Lgr5 is not expressed in adult hematopoietic stem and progenitor cells (HSPCs). Whether Lgr5 is expressed in the embryonic and fetal HSPCs that undergo rapid proliferation is unknown. Here we report the detection of Lgr5 expression in HSPCs in the aorta-gonad-mesonephros (AGM) and fetal liver. We also found that a portion of Lgr5(+) cells expressed the Runx1 gene that is critical for the ontogeny of HSPCs. A small portion of Lgr5(+) cells also expressed HSPC surface markers c-Kit and CD34 in AGM or CD41 in fetal liver. Furthermore, the majority of Lgr5(+) cells expressed Ki67, indicating their proliferating state. Transplantation of fetal liver-derived Lgr5-GFP(+) cells (E12.5) demonstrated that Lgr5-GFP(+) cells were able to reconstitute myeloid and lymphoid lineages in adult recipients, but the engraftment was short-term (4-8 weeks) and 20-fold lower compared with the Lgr5-GFP(-) control. Our data show that Lgr5-expressing cells mark short-term hematopoietic stem and progenitor cells, consistent with the role of Lgr5 in supporting HSPCs rapid proliferation during embryonic and fetal development.
authors: From the Department of Histology and Embryology, Harbin Medical University, Harbin 150086, China, the Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors: Donghua
authors: D
authors: Liu
authors: the Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors: Xi C
authors: XC
authors: He
authors: the Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors: Pengxu
authors: P
authors: Qian
authors: the Institute of Medical Biology, Immunos 138648, Singapore.
authors: Nick
authors: N
authors: Barker
authors: the Stowers Institute for Medical Research, Kansas City, Missouri 64110, the Departments of Anatomy and Cell Biology and.
authors: Paul A
authors: PA
authors: Trainor
authors: the Hubrecht Institute, Utrecht 3584CT, The Netherlands, and.
authors: Hans
authors: H
authors: Clevers
authors: From the Department of Histology and Embryology, Harbin Medical University, Harbin 150086, China, liuhw_11@126.com.
authors: Huiwen
authors: H
authors: Liu
authors: the Stowers Institute for Medical Research, Kansas City, Missouri 64110, Pathology, University of Kansas Medical Center, Kansas City, Kansas 66160 lil@stowers.org.
authors: Linheng
authors: L
authors: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D047108:Embryonic Development; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007930:Leucine / Q000737:chemistry*; D008099:Liver / Q000196:embryology / Q000378:metabolism; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D043562:Receptors, G-Protein-Coupled; D007930:Leucine
keywords: Embryo; Hematopoiesis; Hematopoietic Stem Cells; Lgr5; Progenitor Cells; Proliferation; Stem Cells; Transplantation