title: A partially methylated mannogalactan from hybrid mushroom pfle 1p: purification, structural characterization, and study of immunoactivation.
abstract: A new water-soluble heteropolysaccharide (PS-II) with apparent molecular weight ∼1.65×10(5) Da, was isolated from the fruiting bodies of hybrid mushroom pfle 1p by hot aqueous extraction. It is composed of d-mannose, d-galactose, and 3-O-Me-d-galactose in a molar ratio of 1.0:0.99:1.1. The structural investigation of PS-II has been carried out using acid hydrolysis, methylation analysis, periodate oxidation study, and 1D/2D NMR experiments. Based on the results of these experiments, it was established that PS-II contained a main chain of (1→6) linked α-d-galactopyranosyl residues, one of which was substituted at C-2 by a terminal mannopyranosyl residue and also methylated at C-3 position. This heteropolysaccharide (PS-II) exhibited macrophage activation by NO production as well as in vitro splenocyte and thymocyte stimulation.
authors: Department of Chemistry & Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors: Prasenjit
authors: P
authors: Maity
authors: Department of Chemistry & Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors: Manabendra
authors: M
authors: Pattanayak
authors: Department of Chemistry & Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors: Saikat
authors: S
authors: Maity
authors: Department of Chemistry & Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors: Ashis K
authors: AK
authors: Nandi
authors: Department of Chemistry & Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors: Ipsita K
authors: IK
authors: Sen
authors: Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur 721302, West Bengal, India.
authors: Birendra
authors: B
authors: Behera
authors: Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur 721302, West Bengal, India.
authors: Tapas K
authors: TK
authors: Maiti
authors: Division of Plant Biology, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata 700054, West Bengal, India.
authors: Pijush
authors: P
authors: Mallick
authors: Division of Plant Biology, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata 700054, West Bengal, India.
authors: Samir R
authors: SR
authors: Sikdar
authors: Department of Chemistry & Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India. Electronic address: sirajul_1999@yahoo.com.
authors: Syed S
authors: SS
authors: Islam
mesh_terms: D002240:Carbohydrate Sequence; D002678:Chimera / Q000235:genetics; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005690:Galactose / Q000737:chemistry; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008358:Mannose / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009000:Monocytes / Q000166:cytology / Q000187:drug effects; D020076:Pleurotus / Q000737:chemistry* / Q000235:genetics; D020942:Shiitake Mushrooms / Q000737:chemistry* / Q000235:genetics; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects
chemical_list: D062610:Fungal Polysaccharides; D005685:Galactans; D008358:Mannose; D005690:Galactose
keywords: Heteropolysaccharide; Hybrid mushroom; Immunostimulation; NMR analysis