title: Mapping of optimal CD8 T cell epitopes.
abstract: Defining the optimal epitope of a CD8 T cell response towards a certain antigen is a multistep procedure that requires the performance of peptide truncation design, ELISPOT peptide titration assays, and assessing the HLA class I restriction of the defined epitope via intracellular cytokine staining assays with B cell lines and epitope-specific CD8 T cell lines.
authors: Department of infectious diseases, Medizinische Klinik und Poliklinik IV, Klinikum der Universität München, Campus Innenstadt, Pettenkoferstraße 8a, 80336, Munich, Germany.
authors: Julia
authors: J
authors: Roider
authors: Thomas
authors: T
authors: Vollbrecht
authors: Rika
authors: R
authors: Draenert
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D058501:Enzyme-Linked Immunospot Assay; D018604:Epitope Mapping / Q000379:methods*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006801:Humans
chemical_list: D018984:Epitopes, T-Lymphocyte