title: Ex uno plures: clonal reinforcement drives evolution of a simple microbial community.
abstract: A major goal of genetics is to define the relationship between phenotype and genotype, while a major goal of ecology is to identify the rules that govern community assembly. Achieving these goals by analyzing natural systems can be difficult, as selective pressures create dynamic fitness landscapes that vary in both space and time. Laboratory experimental evolution offers the benefit of controlling variables that shape fitness landscapes, helping to achieve both goals. We previously showed that a clonal population of E. coli experimentally evolved under continuous glucose limitation gives rise to a genetically diverse community consisting of one clone, CV103, that best scavenges but incompletely utilizes the limiting resource, and others, CV101 and CV116, that consume its overflow metabolites. Because this community can be disassembled and reassembled, and involves cooperative interactions that are stable over time, its genetic diversity is sustained by clonal reinforcement rather than by clonal interference. To understand the genetic factors that produce this outcome, and to illuminate the community's underlying physiology, we sequenced the genomes of ancestral and evolved clones. We identified ancestral mutations in intermediary metabolism that may have predisposed the evolution of metabolic interdependence. Phylogenetic reconstruction indicates that the lineages that gave rise to this community diverged early, as CV103 shares only one Single Nucleotide Polymorphism with the other evolved clones. Underlying CV103's phenotype we identified a set of mutations that likely enhance glucose scavenging and maintain redox balance, but may do so at the expense of carbon excreted in overflow metabolites. Because these overflow metabolites serve as growth substrates that are differentially accessible to the other community members, and because the scavenging lineage shares only one SNP with these other clones, we conclude that this lineage likely served as an "engine" generating diversity by creating new metabolic niches, but not the occupants themselves.
authors: Division of Biological Sciences, The University of Montana, Missoula, Montana, United States of America.
authors: Margie
authors: M
authors: Kinnersley
authors: Department of Genetics, Stanford University School of Medicine, Stanford, California, United States of America.
authors: Jared
authors: J
authors: Wenger
authors: Division of Biological Sciences, The University of Montana, Missoula, Montana, United States of America.
authors: Evgueny
authors: E
authors: Kroll
authors: Departments of Molecular, Cellular and Developmental Biology and Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Julian
authors: J
authors: Adams
authors: Department of Genetics, Stanford University School of Medicine, Stanford, California, United States of America.
authors: Gavin
authors: G
authors: Sherlock
authors: Division of Biological Sciences, The University of Montana, Missoula, Montana, United States of America.
authors: Frank
authors: F
authors: Rosenzweig
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D018389:Codon, Nonsense / Q000235:genetics; D019020:Directed Molecular Evolution; D004926:Escherichia coli / Q000235:genetics*; D014644:Genetic Variation; D016680:Genome, Bacterial; D005947:Glucose / Q000235:genetics / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D012641:Selection, Genetic
chemical_list: D018389:Codon, Nonsense; D005947:Glucose; D005990:Glycerol