title: Laser-induced breakdown spectroscopy: a new approach for nanoparticle's mapping and quantification in organ tissue.
abstract: Emission spectroscopy of laser-induced plasma was applied to elemental analysis of biological samples. Laser-induced breakdown spectroscopy (LIBS) performed on thin sections of rodent tissues: kidneys and tumor, allows the detection of inorganic elements such as (i) Na, Ca, Cu, Mg, P, and Fe, naturally present in the body and (ii) Si and Gd, detected after the injection of gadolinium-based nanoparticles. The animals were euthanized 1 to 24 hr after intravenous injection of particles. A two-dimensional scan of the sample, performed using a motorized micrometric 3D-stage, allowed the infrared laser beam exploring the surface with a lateral resolution less than 100 μm. Quantitative chemical images of Gd element inside the organ were obtained with sub-mM sensitivity. LIBS offers a simple and robust method to study the distribution of inorganic materials without any specific labeling. Moreover, the compatibility of the setup with standard optical microscopy emphasizes its potential to provide multiple images of the same biological tissue with different types of response: elemental, molecular, or cellular.
authors: ILM-FENNEC UMR 5306, CNRS - Université Lyon 1; lucie.sancey@univ-lyon1.fr.
authors: Lucie
authors: L
authors: Sancey
authors: ILM-PUBLI UMR 5306, CNRS - Université Lyon 1.
authors: Vincent
authors: V
authors: Motto-Ros
authors: ILM-FENNEC UMR 5306, CNRS - Université Lyon 1.
authors: Shady
authors: S
authors: Kotb
authors: ILM-SOPRANO UMR 5306, CNRS - Université Lyon 1.
authors: Xiaochun
authors: X
authors: Wang
authors: ILM-FENNEC UMR 5306, CNRS - Université Lyon 1.
authors: François
authors: F
authors: Lux
authors: ILM-SOPRANO UMR 5306, CNRS - Université Lyon 1.
authors: Gérard
authors: G
authors: Panczer
authors: ILM-PUBLI UMR 5306, CNRS - Université Lyon 1.
authors: Jin
authors: J
authors: Yu
authors: ILM-FENNEC UMR 5306, CNRS - Université Lyon 1.
authors: Olivier
authors: O
authors: Tillement
mesh_terms: D000818:Animals; D005682:Gadolinium / Q000032:analysis / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry; D007834:Lasers; D053768:Metal Nanoparticles / Q000032:analysis / Q000737:chemistry*; D051379:Mice; D009374:Neoplasms, Experimental / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D005682:Gadolinium