title: Expression of peptide YY by human blood leukocytes.
abstract: Peptide YY is produced by L cells in the mucosa of the distal ileum, colon, and rectum and may have systemic and paracrine functions. We hypothesized that peptide YY is expressed by peripheral blood mononuclear cells. The purpose of the present study was to evaluate the expression of peptide YY mRNA and peptide by peripheral blood mononuclear cells and differentiated THP-1 cells after lipopolysaccharide treatment as an in vitro model of inflammation. Blood was drawn by venipuncture from 18- to 63-year-old healthy male blood donors (n=63); peptide YY mRNA expression levels were detected in peripheral blood mononuclear cells from all healthy male subjects. In 3 subjects, peripheral blood mononuclear cells were cultured for 3 and 24h and peptide YY was detected in the cell culture supernatant. In human monocytic THP-1 cells treated with phorbol-12-myristate-13-acetate to induce differentiation to macrophages, treatment with lipopolysaccharide caused down-regulation of peptide YY mRNA levels. In summary, freshly isolated peripheral blood mononuclear cells from healthy humans expressed peptide YY. In vitro data suggested that peptide YY expression is down-regulated by differentiation of monocytes to macrophages and proinflammatory stimuli.
authors: Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Justus-Liebig-University, Feulgen-Str. 10-12, D-35385 Giessen, Germany. Electronic address: julia.p.holler@chiru.med.uni-giessen.de.
authors: Julia Pia Natascha
authors: JP
authors: Holler
authors: Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Justus-Liebig-University, Feulgen-Str. 10-12, D-35385 Giessen, Germany. Electronic address: Jessica.Schmitz@med-uni-goettingen.de.
authors: Jessica
authors: J
authors: Schmitz
authors: Department of Anesthesiology, Intensive Care and Pain Medicine, Justus-Liebig-University of Giessen and Marburg, Campus Giessen, Rudolf-Bucheim-Straße 7, 35392 Giessen, Germany. Electronic address: Rainer.Roehrig@chiru.med.uni-giessen.de.
authors: Rainer
authors: R
authors: Roehrig
authors: Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Justus-Liebig-University, Feulgen-Str. 10-12, D-35385 Giessen, Germany. Electronic address: julia.p.holler@chiru.med.uni-giessen.de.
authors: Sigrid
authors: S
authors: Wilker
authors: Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Justus-Liebig-University, Feulgen-Str. 10-12, D-35385 Giessen, Germany. Electronic address: andreas.hecker@chiru.med.uni-giessen.de.
authors: Andreas
authors: A
authors: Hecker
authors: Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Justus-Liebig-University, Feulgen-Str. 10-12, D-35385 Giessen, Germany. Electronic address: winfried.padberg@chiru.med.uni-giessen.de.
authors: Winfried
authors: W
authors: Padberg
authors: Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Justus-Liebig-University, Feulgen-Str. 10-12, D-35385 Giessen, Germany. Electronic address: veronika.grau@chiru.med.uni-giessen.de.
authors: Veronika
authors: V
authors: Grau
mesh_terms: D000293:Adolescent; D000328:Adult; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019894:Peptide YY / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D002273:Carcinogens; D019894:Peptide YY; D013755:Tetradecanoylphorbol Acetate
keywords: Differentiation; Inflammation; Neuropeptide Y; Pancreatic polypeptide