title: Enzymatic synthesis of dendritic amphoteric α-glucans by thermostable phosphorylase catalysis.
abstract: This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively. The structure of the products is confirmed by the (1)H NMR analysis. The products exhibit inherent isoelectric points (pIs) determined by the ζ-potential measurement. These materials self-assemble in water at pH = pI to form large aggregates, but disassemble at pH shifted from pI.
authors: Department of Chemistry, Biotechnology, and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima, 890-0065, Japan.
authors: Yusei
authors: Y
authors: Takata
authors: Riko
authors: R
authors: Shimohigoshi
authors: Kazuya
authors: K
authors: Yamamoto
authors: Jun-Ichi
authors: J
authors: Kadokawa
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D055162:Biocatalysis; D004337:Drug Carriers / Q000138:chemical synthesis*; D004795:Enzyme Stability; D005936:Glucans / Q000138:chemical synthesis*; D005944:Glucosamine / Q000031:analogs & derivatives / Q000737:chemistry; D005958:Glucosephosphates / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006358:Hot Temperature; D007526:Isoelectric Point; D009682:Magnetic Resonance Spectroscopy; D006005:Phosphorylases / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D004337:Drug Carriers; D005936:Glucans; D005958:Glucosephosphates; C038515:glucosamine 1-phosphate; D020723:Glucuronic Acid; D006005:Phosphorylases; D005944:Glucosamine
keywords: amphoteric; dendrimers; enzymes; phosphorylase; polysaccharides