title: Performance evaluation of Sysmex XN hematology analyzer in umbilical cord blood: a comparison study with Sysmex XE-2100.
abstract: BACKGROUND
  The Sysmex XN (XN) modular system (Sysmex, Kobe, Japan) is a new automated hematology analyzer equipped with different principles from its previous version, Sysmex XE-2100. We compared the performances of Sysmex XN and XE-2100 in umbilical cord blood (CB) specimens.
  
  
  METHODS
  In 160 CB specimens, complete blood count (CBC) parameters and white blood cells (WBC) differentials were compared between the two analyzers. Their flagging performances for blasts, abnormal/atypical lymphocytes, immature granulocytes and/or left-shift (IG), and nucleated red blood cells (NRBC) counts were compared with manual counts. For the blast flagging, Q values by Sysmex XN were further compared with manual slide review.
  
  
  RESULTS
  Sysmex XN and XE-2100 showed high or very high correlations for most CBC parameters but variable correlations for WBC differentials. Compared with XE-2100, XN showed significantly different flagging performances for blasts, abnormal/atypical lymphocytes, and IG. The flagging efficiency for blasts was significantly better on Sysmex XN than on XE-2100 (85.0% vs. 38.8%): Sysmex XN showed a remarkably increased specificity of blast flag, compromising its sensitivity of blast flag. Among the 24 specimens with blasts (range, 0.5%-1.5%), only one (4.2%) showed a positive Q value.
  
  
  CONCLUSIONS
  This study highlighted the remarkable differences of flagging performances between Sysmex XN and XE-2100 in CB specimens. The Sysmex XN modular system seems to be a suitable and practical option for the CB specimens used for hematopoietic stem cell transplantation as well as for the specimens from neonates.
authors: Hanah
authors: H
authors: Kim
authors: Mina
authors: M
authors: Hur
authors: Sang-Gyeu
authors: SG
authors: Choi
authors: Hee-Won
authors: HW
authors: Moon
authors: Yeo-Min
authors: YM
authors: Yun
authors: Han Sung
authors: HS
authors: Hwang
authors: Han Sung
authors: HS
authors: Kwon
authors: In Sook
authors: IS
authors: Sohn
mesh_terms: D000328:Adult; D001724:Birth Weight; D001772:Blood Cell Count / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007962:Leukocytes / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology; D008875:Middle Aged; D055815:Young Adult