title: Downregulation of chicken interleukin-17 receptor A during Eimeria infection.
abstract: Both interleukin-17A (IL-17A) and IL-17F are proinflammatory cytokines that have an important role in intestinal homeostasis via receptor signaling. These cytokines have been characterized in chickens, but very little is known about their receptors and their functional activity. We provide here the first description of the sequence analysis, bioactivity, and comparative expression analysis of chicken IL-17RA (chIL-17RA) in chickens infected with Salmonella and Eimeria, two major infectious agents of gastrointestinal diseases of poultry of economic importance. A full-length chIL-17RA cDNA with a 2,568-bp coding region was identified from chicken thymus cDNA. chIL-17RA shares ca. 46% identity with mammalian homologues and 29.2 to 31.5% identity with its piscine counterparts. chIL-17RA transcript expression was relatively high in the thymus and in the chicken macrophage cell line HD11. The chIL-17RA-specific small interfering RNA inhibits interleukin-6 (IL-6), IL-8, and IL-1β mRNA expression in chicken embryo fibroblast cells (but not in DF-1 cells) stimulated with chIL-17A or chIL-17F. Interaction between chIL-17RA and chIL-17A was confirmed by coimmunoprecipitation. Downregulation of chIL-17RA occurred in concanavalin A- or lipopolysaccharide-activated splenic lymphocytes but not in poly(I·C)-activated splenic lymphocytes. In Salmonella- and Eimeria-infected chickens, the expression levels of the chIL-17RA transcript were downregulated in intestinal tissues from chickens infected with two Eimeria species, E. tenella or E. maxima, that preferentially infect the cecum and jejunum, respectively. However, chIL-17RA expression was generally unchanged in Salmonella infection. These results suggest that chIL-17RA has an important role in mucosal immunity to intestinal intracellular parasite infections such as Eimeria infection.
authors: College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University, Jinju, South Korea.
authors: Woo H
authors: WH
authors: Kim
authors: College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University, Jinju, South Korea.
authors: Jipseol
authors: J
authors: Jeong
authors: College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University, Jinju, South Korea.
authors: Ae R
authors: AR
authors: Park
authors: College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University, Jinju, South Korea.
authors: Dongjean
authors: D
authors: Yim
authors: College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University, Jinju, South Korea.
authors: Suk
authors: S
authors: Kim
authors: Department of Animal Science, College of Agriculture, Gyeongsang National University, Jinju, South Korea.
authors: Hong H
authors: HH
authors: Chang
authors: Animal Environment Division, National Institute of Animal Science, RDA, Gyeonggi, South Korea.
authors: Seung-Hak
authors: SH
authors: Yang
authors: Department of Orthopaedic Surgery, Gyeongsang National University Hospital, Jinju, South Korea.
authors: Dong-Hee
authors: DH
authors: Kim
authors: Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, U.S. Department of Agriculture, Beltsville, Maryland, USA.
authors: Hyun S
authors: HS
authors: Lillehoj
authors: College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University, Jinju, South Korea wongimin@gnu.ac.kr.
authors: Wongi
authors: W
authors: Min
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics* / Q000469:parasitology; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular / Q000379:methods; D003048:Coccidiosis / Q000235:genetics* / Q000469:parasitology; D018076:DNA, Complementary / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics*; D004539:Eimeria; D005347:Fibroblasts / Q000469:parasitology; D007378:Interleukins / Q000235:genetics; D007422:Intestines / Q000469:parasitology; D008214:Lymphocytes / Q000378:metabolism; D008264:Macrophages / Q000469:parasitology; D008297:Male; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000235:genetics / Q000469:parasitology; D012333:RNA, Messenger / Q000235:genetics; D053722:Receptors, Interleukin-17 / Q000235:genetics*; D012475:Salmonella / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D007378:Interleukins; D012333:RNA, Messenger; D053722:Receptors, Interleukin-17