title: Increased T-cell responses to Epstein-Barr virus with high viral load in patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma.
abstract: The immunological status of patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma (EBV+ DLBCL) without obvious immunodeficiency has not been elucidated. A multicenter prospective study was conducted to assess pretreatment T-cell responses to EBV, EBV-DNA load and anti-EBV antibody in these patients. The proliferative and interferon (IFN)-γ-producing capacity of T-cells in response to autologous B-lymphoblastoid cell lines was determined using carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay. Frequencies of EBV-specific CD4+ T-cells in patients with EBV+ DLBCL (n = 13) were significantly higher than in healthy controls (HCs) (n = 16) after both ex vivo and in vitro stimulation. Frequencies of EBV-specific CD8+ T-cells in patients with EBV+ DLBCL tended to be higher than in HCs after in vitro stimulation. Patients with EBV+ DLBCL also showed increased immunoglobulin G (IgG) responses to lytic EBV-encoded antigens. Pretreatment plasma EBV-DNA level was significantly higher in patients with EBV+ DLBCL than in patients with EBV- DLBCL or HCs. In conclusion, EBV-specific T-cells showed increased reactivity, accompanied by higher levels of plasma virus DNA in patients with EBV+ DLBCL.
authors: Department of Hematology, Fujita Health University School of Medicine , Toyoake , Japan.
authors: Satoko
authors: S
authors: Morishima
authors: Shigeo
authors: S
authors: Nakamura
authors: Kazuhito
authors: K
authors: Yamamoto
authors: Hidemasa
authors: H
authors: Miyauchi
authors: Yoshitoyo
authors: Y
authors: Kagami
authors: Tomohiro
authors: T
authors: Kinoshita
authors: Hiroshi
authors: H
authors: Onoda
authors: Yasushi
authors: Y
authors: Yatabe
authors: Masafumi
authors: M
authors: Ito
authors: Kouichi
authors: K
authors: Miyamura
authors: Hirokazu
authors: H
authors: Nagai
authors: Suzuko
authors: S
authors: Moritani
authors: Isamu
authors: I
authors: Sugiura
authors: Keitaro
authors: K
authors: Tsushita
authors: Hidetsugu
authors: H
authors: Mihara
authors: Kaneyuki
authors: K
authors: Ohbayashi
authors: Sachiko
authors: S
authors: Iba
authors: Nobuhiko
authors: N
authors: Emi
authors: Masataka
authors: M
authors: Okamoto
authors: Seiko
authors: S
authors: Iwata
authors: Hiroshi
authors: H
authors: Kimura
authors: Kiyotaka
authors: K
authors: Kuzushima
authors: Yasuo
authors: Y
authors: Morishima
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D004279:DNA, Viral / Q000097:blood / Q000235:genetics / Q000276:immunology; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000276:immunology* / Q000821:virology; D005260:Female; D005434:Flow Cytometry; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000627:therapeutic use; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D018655:Lymphocyte Count; D016403:Lymphoma, Large B-Cell, Diffuse / Q000150:complications / Q000188:drug therapy / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D019562:Viral Load / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D007371:Interferon-gamma
keywords: EBV-DNA load; EBV-positive diffuse large B-cell lymphoma; IFN-γ secretion; T-cell proliferation; immune response