title: Genetic and pathogenic characteristics of H1 avian and swine influenza A viruses.
abstract: This study examined the potential for cross-species transmission of influenza viruses by comparing the genetic and pathogenic characteristics of H1 avian influenza viruses (AIVs) with different host origins in Korea. Antigenic and phylogenetic analyses of H1 AIVs circulating in Korea provided evidence of genetic similarity between viruses that infect domestic ducks and those that infect wild birds, although there was no relationship between avian and swine viruses. However, there were some relationships between swine and human viral genes. The replication and pathogenicity of the H1 viruses was assessed in chickens, domestic ducks and mice. Viral shedding in chickens was relatively high. Virus was recovered from both oropharyngeal and cloacal swabs up to 5-10 days post-inoculation. The titres of domestic duck viruses in chickens were much higher than those of wild-bird viruses. Both domestic duck and wild-bird viruses replicated poorly in domestic ducks. None of the swine viruses replicated in chickens or domestic ducks; however, six viruses showed relatively high titres in mice, regardless of host origin, and induced clinical signs such as ruffled fur, squatting and weight loss. Thus, although the phylogenetic and antigenic analyses showed no evidence of interspecies transmission between birds and swine, the results suggest that Korean H1 viruses have the potential to cause disease in mammals. Therefore, we should intensify continuous monitoring of avian H1 viruses in mammals and seek to prevent interspecies transmission.
authors: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido, 430-757, Republic of Korea.
authors: Hyun-Mi
authors: HM
authors: Kang
authors: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido, 430-757, Republic of Korea.
authors: Eun-Kyoung
authors: EK
authors: Lee
authors: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido, 430-757, Republic of Korea.
authors: Byung-Min
authors: BM
authors: Song
authors: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido, 430-757, Republic of Korea.
authors: Jipseol
authors: J
authors: Jeong
authors: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido, 430-757, Republic of Korea.
authors: Hye-Ryoung
authors: HR
authors: Kim
authors: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido, 430-757, Republic of Korea.
authors: Eun-Jin
authors: EJ
authors: Choi
authors: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido, 430-757, Republic of Korea.
authors: Yeun-Kyung
authors: YK
authors: Shin
authors: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido, 430-757, Republic of Korea.
authors: Hee-Soo
authors: HS
authors: Lee
authors: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido, 430-757, Republic of Korea.
authors: Youn-Jeong
authors: YJ
authors: Lee
mesh_terms: D000818:Animals; D001717:Birds; D002645:Chickens; D004195:Disease Models, Animal; D004372:Ducks; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D005585:Influenza in Birds / Q000821:virology*; D007251:Influenza, Human / Q000821:virology*; D007723:Korea; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine; D014774:Virulence; D014779:Virus Replication; D017201:Virus Shedding
chemical_list: D012367:RNA, Viral