title: Improved longevity of hematopoiesis in long-term bone marrow cultures and reduced irradiation-induced pulmonary fibrosis in Toll-like receptor-4 deletion recombinant-negative mice.
abstract: OBJECTIVE
  We measured long-term hematopoiesis in continuous bone marrow cultures derived from Toll-like receptor-4 (Tlr4(-/-))(C57BL/6J) mice.
  
  
  METHODS
  We measured hematopoiesis in vitro over 27 weeks in long-term bone marrow cultures from Tlr4(-/-) and control mice, and irradiation-induced pulmonary fibrosis in mice irradiated to 20 Gy to the thorax.
  
  
  RESULTS
  There was a significant increase in the duration of hematopoiesis in long-term bone marrow cultures from Tlr4(-/-) mice in production of total non-adherent cells and day 7 and day 14 multi-lineage colony-forming cells. The histology of bone marrow hematopoietic and stromal cell lines was indistinguishable between different mouse strains. There was no detectable late irradiation pulmonary fibrosis in Tlr4(-/-) mice.
  
  
  CONCLUSIONS
  Homozygous deletion of both alleles of Tlr4, encoding for an inflammatory mediator receptor, improves the duration of hematopoiesis in vitro and reduces irradiation-induced lung fibrosis.
authors: Department of Radiation Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, PA, U.S.A.
authors: Byung Han
authors: BH
authors: Rhieu
authors: Department of Radiation Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, PA, U.S.A.
authors: Michael W
authors: MW
authors: Epperly
authors: Department of Radiation Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, PA, U.S.A.
authors: Shaonan
authors: S
authors: Cao
authors: Department of Radiation Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, PA, U.S.A.
authors: Julie
authors: J
authors: Goff
authors: Department of Radiation Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, PA, U.S.A.
authors: Donna
authors: D
authors: Shields
authors: Department of Radiation Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, PA, U.S.A.
authors: Darcy
authors: D
authors: Franicola
authors: Department of Radiation Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, PA, U.S.A.
authors: Hong
authors: H
authors: Wang
authors: Department of Radiation Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, PA, U.S.A. greenbergerjs@upmc.edu.
authors: Joel S
authors: JS
authors: Greenberger
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D003114:Colony-Forming Units Assay; D004195:Disease Models, Animal; D017353:Gene Deletion; D006410:Hematopoiesis / Q000235:genetics* / Q000528:radiation effects*; D006720:Homozygote; D051379:Mice; D018345:Mice, Knockout; D011658:Pulmonary Fibrosis / Q000209:etiology* / Q000235:genetics; D011827:Radiation; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: D051197:Toll-Like Receptor 4
keywords: Hematopoiesis; TLR4; long-term bone marrow cultures