title: Functional investigations on embryonic stem cells labeled with clinically translatable iron oxide nanoparticles.
abstract: Stem cell based therapies offer significant potential in the field of regenerative medicine. The development of superparamagnetic iron oxide (SPIO) nanoparticle labeling and magnetic resonance imaging (MRI) have been increasingly used to track the transplanted cells, enabling in vivo determination of cell fate. However, the impact of SPIO-labeling on the cell phenotype and differentiation capacity of embryonic stem cells (ESCs) remains unclear. In this study, we wrapped SPIO nanoparticles with stearic acid grafted PEI600, termed as Stearic-LWPEI-SPIO, to generate efficient and non-toxic ESC labeling tools. Our results showed that efficient labeling of ESCs at an optimized low dosage of Stearic-LWPEI-SPIO nanoparticles did not alter the differentiation and self-renewal properties of ESCs. The localization of the transplanted ESCs observed by MRI correlated well with histological studies. These findings demonstrate that Stearic-LWPEI-SPIO nanoparticles have potential to be clinically translatable MRI probes and may enable non-invasive in vivo tracking of ESCs in experimental and clinical settings during cell-based therapies.
authors: Institute of Stem Cell and Regenerative Medicine, Medical College, Xiamen University, Xiamen, 361102, China. xuxq@xmu.edu.cn.
authors: Jing
authors: J
authors: Liu
authors: Liqin
authors: L
authors: Wang
authors: Jianbo
authors: J
authors: Cao
authors: Yue
authors: Y
authors: Huang
authors: Yu
authors: Y
authors: Lin
authors: Xiaoyun
authors: X
authors: Wu
authors: Zhiyong
authors: Z
authors: Wang
authors: Fan
authors: F
authors: Zhang
authors: Xiuqin
authors: X
authors: Xu
authors: Gang
authors: G
authors: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D017690:Cell Transplantation; D053595:Embryonic Stem Cells / Q000166:cytology*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D007501:Iron; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008854:Microscopy, Electron; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology; D010641:Phenotype; D013229:Stearic Acids / Q000737:chemistry; D013724:Teratoma / Q000473:pathology
chemical_list: D005290:Ferric Compounds; D013229:Stearic Acids; C000499:ferric oxide; C031183:stearic acid; D007501:Iron