title: Serum levels of IL-10, IL-17F and IL-33 in patients with asthma: a case-control study.
abstract: OBJECTIVE
  The development of inflammation in asthma involves an intricate network of cytokines that recruit and activate numerous immune cells. This study was aimed to compare serum levels of IL-10, IL-17F, and IL-33 in asthmatic patients and non-asthmatic controls and correlate cytokine levels to asthma severity and various clinical, spirometric, and laboratory variables.
  
  
  METHODS
  Using ELISA, serum levels of IL-10, IL-17F, and IL-33 were evaluated in 44 asthmatics (14 mild persistent, 15 moderate persistent, and 15 severe persistent) and 44 controls.
  
  
  RESULTS
  This is one of the first reports showing a significant difference in serum levels of asthma-associated cytokines, anti-inflammatory IL-10, and pro-inflammatory IL-17F and IL-33, in the same subset of asthmatic patients. Our results showed diminished level of IL-10 and elevated levels of IL-17F and IL-33 in asthmatics than in controls (p < 0.001). Assessment of cytokine levels between subjects of different gender, age group, and BMI showed non-significant differences. Correlation analysis of cytokine levels to clinical variables showed that IL-17F is associated negatively to FVC % predicted (forced vital capacity) and FEV1% predicted (forced expiratory volume in one second) and positively to number of allergens sensitized and FEV1 reversibility. A strong negative correlation was found between IL-10 and IL-33 levels (p = 0.001).
  
  
  CONCLUSIONS
  Negative correlation between IL-10 and IL-33 levels may reflect a converse relationship between anti-inflammatory and pro-inflammatory cytokines in an individually balanced pattern. The association between IL-17F level and asthmatic phenotypes such as reduced FVC and FEV1, higher degree of sensitization, and post-bronchodilator reversibility needs further assessments.
authors: Genetics and Genomics Lab, Department of Studies in Zoology, University of Mysore , Manasagangotri, Mysore, Karnataka , India .
authors: Sareh
authors: S
authors: Raeiszadeh Jahromi
authors: P A
authors: PA
authors: Mahesh
authors: B S
authors: BS
authors: Jayaraj
authors: Subba Rao V
authors: SR
authors: Madhunapantula
authors: Amrutha D
authors: AD
authors: Holla
authors: Sangeetha
authors: S
authors: Vishweswaraiah
authors: Nallur B
authors: NB
authors: Ramachandra
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D020381:Interleukin-17 / Q000097:blood*; D000067596:Interleukin-33; D007378:Interleukins / Q000097:blood*; D008297:Male; D018709:Statistics, Nonparametric; D014797:Vital Capacity
chemical_list: D015415:Biomarkers; C440021:IL17F protein, human; C506536:IL33 protein, human; D020381:Interleukin-17; D000067596:Interleukin-33; D007378:Interleukins; D016753:Interleukin-10
keywords: Biomarkers; immunopathogenesis; phenotype