title: Evaluation of Various Factors Affecting Bioconversion of l-Tyrosine to l-DOPA by Yeast Yarrowia lipolytica-NCIM 3450 Using Response Surface Methodology.
abstract: 3,4-Dihydroxy l-phenylalanine (l-DOPA) is considered a potent drug for the treatment of Parkinson disease. Physical and nutritional parameters where optimized by using Yarrowia lipolytica-NCIM 3450 to accomplished the highest production of l-DOPA. Screenings of critical components were completed by using a Plackett-Burman design, while further optimization was carried out using the Box-Behnken design. The optimized factor levels predicted by the model were pH 6.1, 1.659 g L(-1) yeast extract, 1.491 g L(-1)l-tyrosine and 0.0290 g L(-1) CuSO4. The predicted yield of l-DOPA with these levels was 1.319 g L(-1), while actual yield obtained was 1.273 g L(-1). The statistical analysis revealed that model is significant with F value 19.55 and R(2) value 0.9514. This process resulted in a 3.594-fold increase in the yield of l-DOPA. l-DOPA was confirmed by HPTLC and HPLC analysis. Thus, Yarrowia lipolytica-NCIM 3450 has potential to be a new source for the production of l-DOPA.
authors: Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, 416004 India.
authors: Swati T
authors: ST
authors: Gurme
authors: Department of Microbiology, Shivaji University, Kolhapur, 416004 India.
authors: Shripad N
authors: SN
authors: Surwase
authors: Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, 416004 India.
authors: Sushama A
authors: SA
authors: Patil
authors: Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, 416004 India.
authors: Jyoti P
authors: JP
authors: Jadhav
keywords: RSM; Yarrowia lipolytica; l-DOPA; l-tyrosine