title: Platelet response to serotonin in patients with stable coronary heart disease.
abstract: Patients with heart disease and depression have an increased mortality rate. Both behavioral and biologic factors have been proposed as potential etiologic mechanisms. Given that the pathophysiology of depression is considered to involve disruption in brain serotonergic signaling, we investigated platelet response to serotonin stimulation in patients with stable coronary artery disease (CAD). We enrolled 92 patients with stable CAD. Platelet response to increasing concentrations of serotonin (5-HT), epinephrine-augmented 5-HT, and adenosine diphosphate (ADP) was measured by optical aggregation and flow cytometry. As concentrations of 5-HT and ADP increased, so did the activation and aggregation of the platelets. However, on addition of the highest concentration of 5-HT (30 μM), a significant decrease in platelet activation (p=0.005) was detected by flow cytometry. This contrasts the increase in platelet activation seen with the addition of the highest concentration of ADP. In conclusion, we found increased platelet activation and aggregation with increased concentrations of ADP; however, when platelets are stimulated with a high concentration of 5-HT (30 μM), there is decreased platelet activation. The data demonstrate unique patterns of platelet activation by 5-HT in patients with stable CAD. The cause of this phenomenon is unclear. Our study sheds light on the in vitro response of platelet function to serotonin in patients with stable CAD, which may further the mechanistic understanding of heart disease and depression.
authors: Department of Medicine, Johns Hopkins Medical Institution, Baltimore, Maryland.
authors: Dan A
authors: DA
authors: Kim
authors: Department of Medicine, Johns Hopkins Medical Institution, Baltimore, Maryland.
authors: Willie G
authors: WG
authors: McClure
authors: Department of Medicine, Johns Hopkins Medical Institution, Baltimore, Maryland.
authors: Jordan B
authors: JB
authors: Neighoff
authors: Department of Medicine, Johns Hopkins Medical Institution, Baltimore, Maryland.
authors: Dhananjay
authors: D
authors: Vaidya
authors: Department of Medicine, Johns Hopkins Medical Institution, Baltimore, Maryland. Electronic address: mwillia1@jhmi.edu.
authors: Marlene S
authors: MS
authors: Williams
mesh_terms: D000368:Aged; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000097:blood* / Q000150:complications / Q000175:diagnosis / Q000188:drug therapy; D003863:Depression / Q000097:blood / Q000150:complications / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D004562:Electrocardiography; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D011379:Prognosis; D012189:Retrospective Studies; D012701:Serotonin / Q000008:administration & dosage*; D017366:Serotonin Receptor Agonists / Q000008:administration & dosage
chemical_list: D017366:Serotonin Receptor Agonists; D012701:Serotonin