title: Characterization of the calicheamicin orsellinate C2-O-methyltransferase CalO6.
abstract: Although bacterial iterative type I polyketide synthases are now known to participate in the biosynthesis of a small set of diverse natural products, the subsequent downstream modification of the resulting polyketide products is poorly understood. We report the functional characterization of the putative orsellinic acid C2-O-methyltransferase, which is involved in calicheamicin biosynthesis. This study suggests that C2-O-methylation precedes C3-hydroxylation/methylation and C5-iodination and requires a coenzyme A- or acyl carrier protein-bound substrate.
authors: Center for Pharmaceutical Research and Innovation, Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536 (USA). ssingh555@uky.edu.
authors: Shanteri
authors: S
authors: Singh
authors: Nitin S
authors: NS
authors: Nandurkar
authors: Jon S
authors: JS
authors: Thorson
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D006900:Hydroxylation; D007700:Kinetics; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D008958:Models, Molecular; D048630:Polyketide Synthases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012118:Resorcinols / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D012118:Resorcinols; C501612:orsellinic acid; D048630:Polyketide Synthases; D012436:S-Adenosylmethionine; D008780:Methyltransferases
keywords: AdoMet; S-adenosylmethionine; acyl carrier proteins; iterative PKSs; orsellinic acid; polyketides