title: A putative corticosteroid hormone in Pacific lamprey, Entosphenus tridentatus.
abstract: Great efforts have been put forth to elucidate the mechanisms of the stress response in vertebrates and demonstrate the conserved response across different vertebrate groups, ranging from similarities in the activation of the hypothalamic-pituitary-adrenal axis to the release and role of corticosteroids. There is however, still very little known about stress physiology in the Pacific lamprey (Entosphenus tridentatus), descendants of the earliest vertebrate lineage, the agnathans. In this paper we demonstrate that 11-deoxycortisol, a steroid precursor to cortisol in the steroidogenic pathway, may be a functional corticosteroid in Pacific lamprey. We identified the putative hormone in Pacific lamprey plasma by employing an array of methods such as RIA, HPLC and mass spectrometry analysis. We demonstrated that plasma levels of 11-deoxycortisol significantly increased in Pacific lamprey 0.5 and 1 h after stress exposure and that lamprey corticotropin releasing hormone injections increased circulating levels of 11-deoxycortisol, suggesting that the stress response is under the control of the HPA/I axis as it is in higher vertebrates. A comprehensive understanding of vertebrate stress physiology may help shed light on the evolution of the corticosteroid signaling system within the vertebrate lineage.
authors: Department of Zoology, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
authors: Satbir
authors: S
authors: Rai
authors: Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
authors: András
authors: A
authors: Szeitz
authors: Department of Zoology, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
authors: Brent W
authors: BW
authors: Roberts
authors: Department of Zoology, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
authors: Quill
authors: Q
authors: Christie
authors: Department of Zoology, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
authors: Wesley
authors: W
authors: Didier
authors: Department of Zoology, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
authors: Junho
authors: J
authors: Eom
authors: Fisheries Centre, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada. Electronic address: s.yun@fisheries.ubc.ca.
authors: Sang-Seon
authors: SS
authors: Yun
authors: Department of Zoology, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada; Fisheries Centre, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada. Electronic address: close@zoology.ubc.ca.
authors: David A
authors: DA
authors: Close
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000008:administration & dosage; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000008:administration & dosage; D003350:Cortodoxone / Q000378:metabolism*; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects / Q000378:metabolism*; D007798:Lampreys / Q000378:metabolism*; D010913:Pituitary-Adrenal System / Q000187:drug effects / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D003350:Cortodoxone
keywords: 11-Deoxycortisol; Agnathan; Glucocorticoid; Stress; Stress response