title: Hemeoxygenase 1 partly mediates the anti-inflammatory effect of dieckol in lipopolysaccharide stimulated murine macrophages.
abstract: Eisenia bicyclis is edible brown algae recognized as a rich source of bioactive derivatives mainly phlorotannins reported for their anti-oxidant properties. Of all phlorotannins identified so far, dieckol has shown the most potent effect in anti-inflammatory, radical scavenging and neuroprotective functions. However, whether dieckol up-regulates hemeoxygenase 1 (HO-1) and this mediates its anti-inflammatory effect in murine macrophages remains poorly understood. Dieckol (12.5-50 μM) inhibited nitric oxide production and attenuated inducible nitric oxide synthase, phospho (p)-PI-3K, p-Akt, p-IKK-α/β, p-IκB-α and nuclear p-NF-κBp65 protein expressions, and NF-κB transcriptional activity in LPS (0.1 μg/ml) stimulated murine macrophages. On the other hand, dieckol up-regulated HO-1 which partly mediated its anti-inflammatory effect in murine macrophages. Thus, dieckol appeared to be a potential therapeutic agent against inflammation through HO-1 up-regulation.
authors: Laboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Taddesse
authors: T
authors: Yayeh
authors: Laboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Eun Ju
authors: EJ
authors: Im
authors: Department of Research & Development, Gyeongbuk Institute for Marine Bio-Industry, Uljin 767-813, Republic of Korea; Food Science and Biotechnology Major, Andong National University, Andong 760-749, Republic of Korea.
authors: Tae-Hyung
authors: TH
authors: Kwon
authors: Department of Herbology, College of Korean Medicine, Daegu Haany University, Gyeongsan 712-715, Republic of Korea.
authors: Seong-Soo
authors: SS
authors: Roh
authors: Department of Veterinary Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Suk
authors: S
authors: Kim
authors: Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors: Ji Hye
authors: JH
authors: Kim
authors: Department of Clinical Laboratory Science, Chungbuk Health and Science University, Chungbuk 363-794, Republic of Korea.
authors: Seung-Bok
authors: SB
authors: Hong
authors: Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors: Jae Youl
authors: JY
authors: Cho
authors: Department of Research & Development, Gyeongbuk Institute for Marine Bio-Industry, Uljin 767-813, Republic of Korea. Electronic address: pnh863660@gimb.or.kr.
authors: Nyun-Ho
authors: NH
authors: Park
authors: Laboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: rheemh@knu.ac.kr.
authors: Man Hee
authors: MH
authors: Rhee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D002460:Cell Line; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007249:Inflammation / Q000188:drug therapy*; D008070:Lipopolysaccharides / Q000276:immunology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D051058:Oncogene Protein v-akt / Q000378:metabolism; D000459:Phaeophyceae / Q000276:immunology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001572:Benzofurans; D008070:Lipopolysaccharides; D016328:NF-kappa B; C503840:dieckol; D009569:Nitric Oxide; D051547:Heme Oxygenase-1; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt
keywords: Dieckol; HO-1; Inflammation; Murine macrophages