title: Dammarane triterpenes as potential SIRT1 activators from the leaves of Panax ginseng.
abstract: During a search for SIRT1 activators originating in nature, three new dammarane triterpenes, 6α,20(S)-dihydroxydammar-3,12-dione-24-ene (1), 6α,20(S),24(S)-trihydroxydammar-3,12-dione-25-ene (2), and 6α,20(S),25-trihydroxydammar-3,12-dione-23-ene (3), as well as two known triterpenes, dammar-20(22),24-diene-3β,6α,12β-triol (4) and 20(S)-ginsenoside Rg3 (5), were isolated from Panax ginseng leaves. Compounds 1 and 3-5 showed potential as SIRT1 activators, as analyzed by in vitro enzyme-based SIRT1-NAD/NADH and SIRT1-p53 luciferase cell-based assays. They were also found to increase the level of NAD(+)/NADH ratio in HEK293 cells. This study presents a new class of chemical entities that may be able to be developed as SIRT1 activators for antiaging and treatment of age-associated diseases.
authors: Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University , Seoul 151-742, Republic of Korea.
authors: Jun-Li
authors: JL
authors: Yang
authors: Thi-Kim-Quy
authors: TK
authors: Ha
authors: Basanta
authors: B
authors: Dhodary
authors: Kuk-Hwa
authors: KH
authors: Kim
authors: Junsoo
authors: J
authors: Park
authors: Chul-Ho
authors: CH
authors: Lee
authors: Young-Choong
authors: YC
authors: Kim
authors: Won-Keun
authors: WK
authors: Oh
mesh_terms: D036145:Ginsenosides / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D005894:Panax / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D056564:Sirtuin 1 / Q000187:drug effects*; D013237:Stereoisomerism; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000097669:Dammaranes
chemical_list: D036145:Ginsenosides; D056564:Sirtuin 1; D014315:Triterpenes; D000097669:Dammaranes; C097367:ginsenoside Rg3