title: Lipolytic and antiadipogenic effects of (3,3-dimethylallyl) halfordinol on 3T3-L1 adipocytes and high fat and fructose diet induced obese C57/BL6J mice.
abstract: Aegle marmelos Correa., (Rutaceae) is a medium sized tree distributed in South East Asia and used traditionally for the management of obestiy and diabetes. In this study the lipolytic and antiadipogenic effects of (3,3-dimethylallyl) halfordinol (Hfn) isolated from leaves of A. marmelos have been investigated. Intracellular lipid accumulation was measured by oil red O staining and glycerol secretion. The expression of genes related to adipocyte differentiation was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). Hfn decreased intracellular triglyceride accumulation and increased glycerol release in a dose dependent manner (5-20 μg/ml) in differentiated 3T3-L1 adipocytes. In high fat diet fed C57/BL 6J mice, treatment with Hfn for four weeks reduced plasma glucose, insulin and triglyceride levels and showed a significant reduction in total adipose tissue mass by 37.85% and visceral adipose tissue mass by 62.99% at 50mg/kg b.w. concentration. RT-PCR analyses indicated that Hfn decreased the expression of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT enhancer binding protein α (CEBPα) and increased the expression of sterol regulatory enzyme binding protein (SREBP-1c), peroxisome proliferator-activated receptor α (PPARα), Adiponectin and Glucose transporter protein 4 (GLUT4) compared to the high fat diet group. These results suggested that Hfn decreased adipocyte differentiation and stimulated lipolysis of adipocytes. This study justifies the folklore medicinal uses and claims about the therapeutic values of this plant for the management of insulin resistance and obesity.
authors: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Nungambakkam, Chennai 600034, India.
authors: Munisankar
authors: M
authors: Saravanan
authors: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Nungambakkam, Chennai 600034, India.
authors: Perumal
authors: P
authors: Pandikumar
authors: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Nungambakkam, Chennai 600034, India.
authors: Subramaniam
authors: S
authors: Saravanan
authors: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Nungambakkam, Chennai 600034, India.
authors: Erenius
authors: E
authors: Toppo
authors: Department of Veterinary Pathology, Madras Veterinary College, Vepery, Chennai 600007, India.
authors: Natesan
authors: N
authors: Pazhanivel
authors: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Nungambakkam, Chennai 600034, India. Electronic address: entolc@hotmail.com.
authors: Savarimuthu
authors: S
authors: Ignacimuthu
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects; D000498:Allyl Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001786:Blood Glucose / Q000032:analysis; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D059305:Diet, High-Fat; D005632:Fructose; D005990:Glycerol / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D007328:Insulin / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000097:blood / Q000188:drug therapy / Q000235:genetics; D010080:Oxazoles / Q000494:pharmacology* / Q000627:therapeutic use; D047493:PPAR alpha / Q000235:genetics; D047495:PPAR gamma / Q000235:genetics; D008517:Phytotherapy; D018515:Plant Leaves; D027821:Rutaceae; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics; D014280:Triglycerides / Q000378:metabolism
chemical_list: C000602297:(3,3-dimethylallyl)halfordinol; D000498:Allyl Compounds; D019440:Anti-Obesity Agents; D001786:Blood Glucose; D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D007004:Hypoglycemic Agents; D007328:Insulin; D010080:Oxazoles; D047493:PPAR alpha; D047495:PPAR gamma; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D014280:Triglycerides; D005632:Fructose; D005990:Glycerol
keywords: Insulin resistance; Lipolysis; Obesity; Type 2 diabetes mellitus