title: A preliminary study of the relationship between breast cancer metastasis and loss of heterozygosity by using exome sequencing.
abstract: We explored the feasibility of studying loss of heterozygosity (LOH) by using exome sequencing and compared the differences in genetic LOH between primary breast tumors and metastatic lesions. Exome sequencing was conducted to investigate the genetic LOH in the peripheral blood, a primary tumor, and a metastatic lesion from the same patient. LOH was observed in 30 and 48 chromosomal loci of the primary tumor and metastatic lesion, respectively. The incidence of LOH was the highest on chromosome 19, followed by chromosomes 14, 3, and 11 in the metastatic lesion. Among these 'hot' regions, LOH was observed for multiple genes of the CECAM, MMP and ZNF families. Therefore, the use of exome sequencing for studying LOH is feasible. More members of gene families appeared with LOH in 'hot' regions, suggesting that these gene families had synergistic effects in tumorigenesis.
authors: Department of Breast Surgery, the Affiliated Tumor Hospital of Guangzhou Medical University, Guangzhou 510095, China.
authors: Hongsheng
authors: H
authors: Li
authors: The tumor Research Institute of Guangzhou Medical University, Guangzhou 510095, China.
authors: Bo
authors: B
authors: Yang
authors: School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
authors: Ke
authors: K
authors: Xing
authors: Department of Breast Surgery, the Affiliated Tumor Hospital of Guangzhou Medical University, Guangzhou 510095, China.
authors: Nangui
authors: N
authors: Yuan
authors: Department of Breast Surgery, the Affiliated Tumor Hospital of Guangzhou Medical University, Guangzhou 510095, China.
authors: Bo
authors: B
authors: Wang
authors: School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
authors: Zhenyu
authors: Z
authors: Chen
authors: Department of Breast Surgery, the Affiliated Tumor Hospital of Guangzhou Medical University, Guangzhou 510095, China.
authors: Weixing
authors: W
authors: He
authors: Department of Breast Surgery, the Affiliated Tumor Hospital of Guangzhou Medical University, Guangzhou 510095, China.
authors: Jie
authors: J
authors: Zhou
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D059472:Exome / Q000235:genetics*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009362:Neoplasm Metastasis; D010865:Pilot Projects