title: Wide-field hyperspectral 3D imaging of functionalized gold nanoparticles targeting cancer cells by reflected light microscopy.
abstract: We present a new hyperspectral reflected light microscopy system with a scanned broadband supercontinuum light source. This wide-field and low phototoxic hyperspectral imaging system has been successful for performing spectral three-dimensional (3D) localization and spectroscopic identification of CD44-targeted PEGylated AuNPs in fixed cell preparations. Such spatial and spectral information is essential for the improvement of nanoplasmonic-based imaging, disease detection and treatment in complex biological environment. The presented system can be used for real-time 3D NP tracking as spectral sensors, thus providing new avenues in the spatio-temporal characterization and detection of bioanalytes. 3D image of the distribution of functionalized AuNPs attached to CD44-expressing MDA-MB-231 human cancer cells.
authors: Sergiy
authors: S
authors: Patskovsky
authors: Eric
authors: E
authors: Bergeron
authors: David
authors: D
authors: Rioux
authors: Michel
authors: M
authors: Meunier
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D004867:Equipment Design; D015972:Gene Expression Regulation, Neoplastic; D006046:Gold / Q000737:chemistry*; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology / Q000378:metabolism; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D053768:Metal Nanoparticles; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D018960:Hyaluronan Receptors; D011092:Polyethylene Glycols; D006046:Gold
keywords: Hyperspectral imaging; biomarkers; cancer stem cell; monoclonal antibody; nanoparticles; supercontinuum