title: Identification and analysis of an intracellular Cu/Zn superoxide dismutase from Sepiella maindroni under stress of Vibrio harveyi and Cd2+.
abstract: Superoxide dismutases (SODs) are ubiquitous family of metalloenzymes involved in protecting organisms from excess reactive oxygen species damage. In this paper, a novel intracellular Cu/ZnSOD from Sepiella maindroni (designated as SmSOD) was identified and characterized. The full-length cDNA sequence of SmSOD (GenBank accession No. KF908850) was 709 bp containing an open reading frame (ORF) of 459 bp, encoding 153 amino acid residues peptide with predicted pI/MW (6.02/15.75 kDa), a 131 bp-5'- and 116 bp-3'- untranslated region (UTR). BLASTn analysis and phylogenetic relationship strongly suggested that the sequence shared high similarity with known Cu/Zn SODs. Several highly conserved motifs, including two typical Cu/Zn SOD family domains, two conserved Cu-/Zn-binding sites (H-47, H-49, H-64, H-120 for Cu binding, and H-64, H-72, H-81, D-84 for Zn binding) and intracellular disulfide bond (C-58 and C-146), were also identified in SmSOD. Time-dependent mRNA expression of SmSOD in hepatopancreas was recorded by quantitative real-time RT-PCR after Vibrio harveyi injection and Cd(2+) exposure. The results indicated that SmSOD was an acute-phase protein involved in the immune responses against pathogens and biological indicator for metal contaminants in aquatic environment.
authors: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors: Jian-yu
authors: JY
authors: He
authors: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors: Chang-feng
authors: CF
authors: Chi
authors: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China. Electronic address: liuhuihui2004@126.com.
authors: Hui-hui
authors: HH
authors: Liu
mesh_terms: D000818:Animals; D002104:Cadmium / Q000378:metabolism; D018076:DNA, Complementary; D043143:Hepatopancreas; D008969:Molecular Sequence Data; D010802:Phylogeny; D049833:Sepia / Q000201:enzymology* / Q000276:immunology* / Q000382:microbiology; D013482:Superoxide Dismutase / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D014733:Vibrio / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; D002104:Cadmium; D013482:Superoxide Dismutase
keywords: Copper/zinc superoxide dismutase (Cu/Zn-SOD); Reactive oxygen species (ROS); Sepiella maindroni