title: Physiological-temperature distance measurement in nucleic acid using triarylmethyl-based spin labels and pulsed dipolar EPR spectroscopy.
abstract: Resolving the nanometer-scale structure of biomolecules in natural conditions still remains a challenging task. We report the first distance measurement in nucleic acid at physiological temperature using electron paramagnetic resonance (EPR). The model 10-mer DNA duplex has been labeled with reactive forms of triarylmethyl radicals and then immobilized on a sorbent in water solution and investigated by double quantum coherence EPR. We succeeded in development of optimal triarylmethyl-based labels, approach for site-directed spin labeling and efficient immobilization procedure that, working together, allowed us to measure as long distances as ~4.6 nm with high accuracy at 310 K (37 °C).
authors: Institute of Chemical Biology and Fundamental Medicine, §International Tomography Center, and #N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of the Sciences (SB RAS) , Novosibirsk 630090, Russia.
authors: Georgiy Yu
authors: GY
authors: Shevelev
authors: Olesya A
authors: OA
authors: Krumkacheva
authors: Alexander A
authors: AA
authors: Lomzov
authors: Andrey A
authors: AA
authors: Kuzhelev
authors: Olga Yu
authors: OY
authors: Rogozhnikova
authors: Dmitry V
authors: DV
authors: Trukhin
authors: Tatiana I
authors: TI
authors: Troitskaya
authors: Victor M
authors: VM
authors: Tormyshev
authors: Matvey V
authors: MV
authors: Fedin
authors: Dmitrii V
authors: DV
authors: Pyshnyi
authors: Elena G
authors: EG
authors: Bagryanskaya
mesh_terms: D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals / Q000737:chemistry; D008958:Models, Molecular; D009589:Nitrogen Oxides / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D013113:Spin Labels
chemical_list: D005609:Free Radicals; D009589:Nitrogen Oxides; D009696:Nucleic Acids; D009841:Oligonucleotides; D013113:Spin Labels