title: Fine-mapping and selective sweep analysis of QTL for cold tolerance in Drosophila melanogaster.
abstract: There is a growing interest in investigating the relationship between genes with signatures of natural selection and genes identified in QTL mapping studies using combined population and quantitative genetics approaches. We dissected an X-linked interval of 6.2 Mb, which contains two QTL underlying variation in chill coma recovery time (CCRT) in Drosophila melanogaster from temperate (European) and tropical (African) regions. This resulted in two relatively small regions of 131 kb and 124 kb. The latter one co-localizes with a very strong selective sweep in the European population. We examined the genes within and near the sweep region individually using gene expression analysis and P-element insertion lines. Of the genes overlapping with the sweep, none appears to be related to CCRT. However, we have identified a new candidate gene of CCRT, brinker, which is located just outside the sweep region and is inducible by cold stress. We discuss these results in light of recent population genetics theories on quantitative traits.
authors: Section of Evolutionary Biology, Department of Biology II, Ludwig-Maximilian University of Munich, 82152 Planegg-Martinsried, Germany wilches@bio.lmu.de.
authors: Ricardo
authors: http://orcid.org/0000-0002-7862-1507
authors: R
authors: Wilches
authors: Section of Evolutionary Biology, Department of Biology II, Ludwig-Maximilian University of Munich, 82152 Planegg-Martinsried, Germany.
authors: Susanne
authors: S
authors: Voigt
authors: Section of Evolutionary Biology, Department of Biology II, Ludwig-Maximilian University of Munich, 82152 Planegg-Martinsried, Germany.
authors: Pablo
authors: P
authors: Duchen
authors: Section of Evolutionary Biology, Department of Biology II, Ludwig-Maximilian University of Munich, 82152 Planegg-Martinsried, Germany.
authors: Stefan
authors: S
authors: Laurent
authors: Section of Evolutionary Biology, Department of Biology II, Ludwig-Maximilian University of Munich, 82152 Planegg-Martinsried, Germany.
authors: Wolfgang
authors: W
authors: Stephan
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000349:Africa; D000818:Animals; D002874:Chromosome Mapping; D003080:Cold Temperature; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology; D005060:Europe; D005260:Female; D015870:Gene Expression; D017344:Genes, Insect; D050172:Genes, X-Linked; D040641:Quantitative Trait Loci
keywords: QTL; brinker; cold tolerance; fine-mapping; selective sweep