title: Comparative genome analysis and molecular epidemiology of the reemerging porcine epidemic diarrhea virus strains isolated in Korea.
abstract: Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family, is an enveloped, positive-sense, single-stranded RNA virus, which causes severe diarrhea and dehydration in suckling pigs. We detected three PEDV strains from ten small intestine samples from piglets with acute diarrhea and we determined the complete genome sequences of the reemerging Korean PEDV field isolates, except for the noncoding regions from both ends. The complete genome sequences of the strains were identical or almost identical (one synonymous single-nucleotide polymorphism (SNP) in the ORF1a/1b genomic sequence). Interestingly, comparative genome analysis of recent Korean PEDV isolates and other strains revealed that the complete genome sequences of recent Korean strains were almost identical (99.9%) to those of the US PEDV strains isolated in 2013. These results suggest that the three reemerging Korean strains are distinct from previous endemic Korean PEDV strains and has been recently introduced into Korea from oversea with high likelihood.
authors: College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Jong-Chul
authors: JC
authors: Choi
authors: College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Kun-Kyu
authors: KK
authors: Lee
authors: Sam Hwa Breeding Agri., Inc, 435-3 Shin Jin Ri, Kwang Chun Eup, Hong Sung, Chung Nam, Republic of Korea.
authors: Jae Ho
authors: JH
authors: Pi
authors: College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Seung-Yong
authors: SY
authors: Park
authors: College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Chang-Seon
authors: CS
authors: Song
authors: College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: In-Soo
authors: IS
authors: Choi
authors: College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Joong-Bok
authors: JB
authors: Lee
authors: College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Dong-Hun
authors: DH
authors: Lee
authors: College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: odssey@konkuk.ac.kr.
authors: Sang-Won
authors: SW
authors: Lee
mesh_terms: D000818:Animals; D021821:Communicable Diseases, Emerging; D018352:Coronavirus Infections / Q000662:veterinary*; D016679:Genome, Viral; D023281:Genomics; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D056910:Republic of Korea / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology*
chemical_list: D012367:RNA, Viral
keywords: Animal virus; Complete genome; Coronavirus; Phylogenetic analysis; Porcine epidemic diarrhea virus