title: Sustained Delivery of Chondroitinase ABC from Hydrogel System.
abstract: In the injured spinal cord, chondroitin sulfate proteoglycans (CSPGs) are the principal responsible of axon growth inhibition and they contribute to regenerative failure, promoting glial scar formation. Chondroitinase ABC (chABC) is known for being able to digest proteoglycans, thus degrading glial scar and favoring axonal regrowth. However, its classic administration is invasive, infection-prone and clinically problematic. An agarose-carbomer (AC1) hydrogel, already used in SCI repair strategies, was here investigated as a delivery system capable of an effective chABC administration: the material ability to include chABC within its pores and the possibility to be injected into the target tissue were firstly proved. Subsequently, release kinetic and the maintenance of enzymatic activity were positively assessed: AC1 hydrogel was thus confirmed to be a feasible tool for chABC delivery and a promising device for spinal cord injury topic repair strategies.
authors: Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, via Mancinelli 7, Milan 20131, Italy. filippo.rossi@mail.polimi.it.
authors: Filippo
authors: F
authors: Rossi
authors: Department of Neuroscience, Mario Negri Institute for Pharmacological Research, via La Masa 19, Milan 20156, Italy. pietro.veglianese@marionegri.it.
authors: Pietro
authors: P
authors: Veglianese
authors: Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, via Mancinelli 7, Milan 20131, Italy. marco.santoro@chem.polimi.it.
authors: Marco
authors: M
authors: Santoro
authors: Department of Neuroscience, Mario Negri Institute for Pharmacological Research, via La Masa 19, Milan 20156, Italy. simonetta.papa@marionegri.it.
authors: Simonetta
authors: S
authors: Papa
authors: Department of Neuroscience, Mario Negri Institute for Pharmacological Research, via La Masa 19, Milan 20156, Italy. cristina.rogora@marionegri.it.
authors: Cristina
authors: C
authors: Rogora
authors: Department of Neuroscience, Mario Negri Institute for Pharmacological Research, via La Masa 19, Milan 20156, Italy. valentina.delloro@marionegri.it.
authors: Valentina
authors: V
authors: Dell'Oro
authors: Department of Neuroscience, Mario Negri Institute for Pharmacological Research, via La Masa 19, Milan 20156, Italy. gianluigi.forloni@marionegri.it.
authors: Gianluigi
authors: G
authors: Forloni
authors: Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, via Mancinelli 7, Milan 20131, Italy. maurizio.masi@polimi.it.
authors: Maurizio
authors: M
authors: Masi
authors: Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, via Mancinelli 7, Milan 20131, Italy. giuseppe.perale@polimi.it.
authors: Giuseppe
authors: G
authors: Perale