title: Model carcinogen adsorption dynamics of DNA gel.
abstract: We have derived theoretical equations describing the adsorption of carcinogen to gels in an immersion medium containing carcinogens. The theory was developed for a cylindrical boundary condition under the assumption of a carcinogen diffusion-limited process combined with the "moving boundary picture (Furusawa et al., 2007)". The time course of the adsorbed carcinogen layer thickness and that of the carcinogen concentration in an immersion medium were expressed by a set of scaled variables, and the asymptotic behavior in the initial stage was derived. Experiments based on the theory were performed using a DNA gel sandwiched between a set of coverglasses in a medium containing acridine orange (AO). The boundary between the AO-adsorbed gel layer and AO-nonadsorbed gel layer was traced during the immersion. The time courses of the AO-adsorbed gel layer thickness and the AO concentration in the immersion medium were well explained by the theory, and the number ratio of the total AO molecules to the adsorption sites in the DNA gel was determined.
authors: Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
authors: Naoko
authors: N
authors: Tomita
authors: Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
authors: Daisuke
authors: D
authors: Naito
authors: Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
authors: Isamu
authors: I
authors: Rokugawa
authors: Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
authors: Takao
authors: T
authors: Yamamoto
authors: Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Tenjin-cho, Kiryu, Gunma 376-8515, Japan. Electronic address: dobashi@gunma-u.ac.jp.
authors: Toshiaki
authors: T
authors: Dobashi
mesh_terms: D000165:Acridine Orange / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D002273:Carcinogens / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D012474:Salmon; D012996:Solutions; D013997:Time Factors
chemical_list: D002273:Carcinogens; D005782:Gels; D012996:Solutions; D004247:DNA; D000165:Acridine Orange
keywords: Adsorption; DNA; Dynamics; Gel; Model carcinogen