title: Rhizobium populi sp. nov., an endophytic bacterium isolated from Populus euphratica.
abstract: An endophytic bacterium, designated K-38(T), was isolated from the storage liquid in the stems of Populus euphratica trees at the ancient Ugan River in Xinjiang, PR China. Strain K-38(T) was found to be rod-shaped, Gram-stain-negative, aerobic, non-motile and non-spore-forming. Strain K-38(T) grew at temperatures of 25-37 °C (optimum, 28 °C), at pH 6.0-9.0 (optimum, pH 7.5) and in the presence of 0-3 % (w/v) NaCl with 1 % as the optimum concentration for growth. According to phylogenetic analysis based on 16S rRNA gene sequences, strain K-38(T) was assigned to the genus Rhizobium with highest 16S rRNA gene sequence similarity of 97.2 % to Rhizobium rosettiformans W3(T), followed by Rhizobium nepotum 39/7(T) (96.5 %) and Rhizobium borbori DN316(T) (96.2 %). Phylogenetic analysis of strain K-38(T) based on the protein coding genes recA, atpD and nifH confirmed (similarities were less than 90 %) it to be a representative of a distinctly delineated species of the genus Rhizobium. The DNA G+C content was determined to be 63.5 mol%. DNA-DNA relatedness between K-38(T) and R. rosettiformans W3(T) was 48.4 %, indicating genetic separation of strain K-38(T) from the latter strain. The major components of the cellular fatty acids in strain K-38(T) were revealed to be summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c; 57.2 %), C16 : 0 (13.6 %) and summed feature 2 (comprising C12 : 0 aldehyde, C14 : 0 3-OH/iso-C16 : 1 I and/or unknown ECL 10.928; 11.0 %). Polar lipids of strain K-38(T) include phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminophospholipids and two unidentified phospholipids. Q-10 was the major quinone in strain K-38(T). Based on phenotypic, chemotaxonomic and phylogenetic properties, strain K-38(T) represents a novel species of the genus Rhizobium, for which the name Rhizobium populi sp. nov. is proposed. The type strain is K-38(T) ( = CCTCC AB 2013068(T) = NRRL B-59990(T) = JCM 19159(T)).
authors: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors: Manziram
authors: M
authors: Rozahon
authors: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors: Nurimangul
authors: N
authors: Ismayil
authors: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors: Buayshem
authors: B
authors: Hamood
authors: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors: Raziya
authors: R
authors: Erkin
authors: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors: Mehfuzem
authors: M
authors: Abdurahman
authors: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors: Hormathan
authors: H
authors: Mamtimin
authors: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors: Muhtar
authors: M
authors: Abdukerim
authors: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
authors: Rup
authors: R
authors: Lal
authors: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors: Erkin
authors: E
authors: Rahman
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S