title: Phosphorylation stoichiometries of human eukaryotic initiation factors.
abstract: Eukaryotic translation initiation factors are the principal molecular effectors regulating the process converting nucleic acid to functional protein. Commonly referred to as eIFs (eukaryotic initiation factors), this suite of proteins is comprised of at least 25 individual subunits that function in a coordinated, regulated, manner during mRNA translation. Multiple facets of eIF regulation have yet to be elucidated; however, many of the necessary protein factors are phosphorylated. Herein, we have isolated, identified and quantified phosphosites from eIF2, eIF3, and eIF4G generated from log phase grown HeLa cell lysates. Our investigation is the first study to globally quantify eIF phosphosites and illustrates differences in abundance of phosphorylation between the residues of each factor. Thus, identification of those phosphosites that exhibit either high or low levels of phosphorylation under log phase growing conditions may aid researchers to concentrate their investigative efforts to specific phosphosites that potentially harbor important regulatory mechanisms germane to mRNA translation.
authors: Department of Molecular and Cellular Biology, University of California at Davis, Davis, CA 95616, USA. aaandaya@ucdavis.edu.
authors: Armann
authors: A
authors: Andaya
authors: Department of Molecular and Cellular Biology, University of California at Davis, Davis, CA 95616, USA. nvilla@ucdavis.edu.
authors: Nancy
authors: N
authors: Villa
authors: Department of Molecular and Cellular Biology, University of California at Davis, Davis, CA 95616, USA. wtjia@ucdavis.edu.
authors: Weitao
authors: W
authors: Jia
authors: Department of Molecular and Cellular Biology, University of California at Davis, Davis, CA 95616, USA. csfraser@ucdavis.edu.
authors: Christopher S
authors: CS
authors: Fraser
authors: Department of Molecular and Cellular Biology, University of California at Davis, Davis, CA 95616, USA. jaleary@ucdavis.edu.
authors: Julie A
authors: JA
authors: Leary
mesh_terms: D039642:Eukaryotic Initiation Factors / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational
chemical_list: D039642:Eukaryotic Initiation Factors