title: Roussoella percutanea, a novel opportunistic pathogen causing subcutaneous mycoses.
abstract: We report the isolation of a novel agent of subcutaneous mycosis from a 45-year-old Indian male immigrant in the United States. Phylogenetic analysis of partial small ribosomal subunit and large ribosomal subunit, internal transcribed spacer, partial translation elongation factor (TEF1), and RNA polymerase second largest subunit (rPB2) loci revealed that the strain was identical to another isolate previously reported as "Madurella mycetomatis." Both strains clustered in the order Pleosporales, nested in the family Arthopyreniaceae/Roussoellaceae. The fungus differed from known species and hence a new taxon, Roussoella percutanea, is introduced, typified by a strain that showed delayed production of pycnidial conidiomata. Antifungal susceptibility testing suggested that the new species is resistant to echinocandins and flucytosine, with variable results with azoles and amphotericin B.
authors: Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, The Netherlands.
authors: Sarah A
authors: SA
authors: Ahmed
authors: California Institute for Medical Research, San Jose, and Division of Infectious Diseases, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA stevens@stanford.edu.
authors: David A
authors: DA
authors: Stevens
authors: Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands.
authors: Wendy W J
authors: WW
authors: van de Sande
authors: Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.
authors: Jacques F
authors: JF
authors: Meis
authors: Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, The Netherlands Peking University Health Science Center, Research Center for Medical Mycology, Beijing, China; Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China; Shanghai Institute of Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, China; Basic Pathology Department, Federal University of Paraná State, Curitiba, Paraná, Brazil; King Abdulaziz University, Jeddah, Saudi Arabia.
authors: G S
authors: GS
authors: de Hoog
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D001203:Ascomycota / Q000145:classification* / Q000302:isolation & purification*; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003881:Dermatomycoses / Q000382:microbiology*; D025141:Drug Resistance, Fungal; D054242:Emigrants and Immigrants; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009894:Opportunistic Infections / Q000382:microbiology*; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D012319:RNA Polymerase II / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014481:United States
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D020648:Peptide Elongation Factor 1; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D012319:RNA Polymerase II
keywords: Pleosporales; Roussoella; antifungal susceptibility; phylogeny; subcutaneous mycosis