title: Myosins Are Differentially Expressed under Oxidative Stress in Chronic Streptozotocin-Induced Diabetic Rat Brains.
abstract: Diabetes mellitus is a disease characterized by persistent hyperglycemia, which may lead to brain tissue damage due to oxidative stress and also contributes to neuronal death and changes in synaptic transmission. This study evaluated the effect of oxidative stress and the use of antioxidants supplementation on myosins expression levels in the brains of chronic diabetic rats induced by streptozotocin. Lipid peroxidation, antioxidant enzymes activities, and myosins-IIB and -Va expressions at transcriptional and translational levels were examined after 90 days induction. The chronic effect of the diabetes led to the upregulation of superoxide dismutase (SOD) and catalase (CAT) activities, and the downregulation of glutathione peroxidase (GPx), but there was no statistically significant increase in the malondialdehyde (MDA) levels. These alterations were accompanied by high myosin-IIB and low myosin-Va expressions. Although the antioxidant supplementation did not interfere on MDA levels, the oxidative stress caused by chronic hyperglycemia was reduced by increasing SOD and restoring CAT and GPx activities. Interestingly, after supplementation, diabetic rats recovered only myosin-Va protein levels, without interfering on myosins mRNA levels expressed in diabetic rat brains. Our results suggest that antioxidant supplementation reduces oxidative stress and also regulates the myosins protein expression, which should be beneficial to individuals with diabetes/chronic hyperglycemia.
authors: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil ; Federal University of Juiz de Fora, Governador Valadares, MG, Brazil.
authors: Luciana Karen
authors: LK
authors: Calábria
authors: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
authors: Alice
authors: A
authors: Vieira da Costa
authors: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
authors: Renato José
authors: RJ
authors: da Silva Oliveira
authors: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
authors: Simone
authors: S
authors: Ramos Deconte
authors: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
authors: Rafael
authors: 0000-0003-3650-9354
authors: R
authors: Nascimento
authors: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
authors: Washington João
authors: WJ
authors: de Carvalho
authors: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil ; Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors: Vanessa Neves
authors: VN
authors: de Oliveira
authors: Department of Chemistry, Physic, and Mathematic, University of Ribeirão Preto, Ribeirão Preto, SP, Brazil.
authors: Carlos Alberto
authors: 0000-0003-4642-2551
authors: CA
authors: Arcaro Filho
authors: Department of Chemistry, Physic, and Mathematic, University of Ribeirão Preto, Ribeirão Preto, SP, Brazil.
authors: Luciana
authors: L
authors: Rezende Alves de Oliveira
authors: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
authors: Luiz Ricardo
authors: LR
authors: Goulart
authors: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
authors: Foued Salmen
authors: FS
authors: Espindola