title: [Effects of heishunpian total alkaloids on Cassia acutifolia induced mice diarrhea and contraction of isolated intestinal smooth muscle in rats].
abstract: OBJECTIVE
  To observe the effects of Heishunpian total alkaloids on Cassia acutifolia induced mice diarrhea and contraction of isolated intestinal smooth muscle in rats.
  
  
  METHODS
  The experiment was carried out with Cassia acutifolia induced mice diarrhea model, small intestinal propulsive rate in mice was used to valuate the effect of Heishunpian total alkaloids on diarrhea mice; The effects of Heishunpian total alkaloids on contraction of isolated intestinal smooth muscle in rats and its mechanism were investigated by monitoring amplitude and tension of isolated intestinal smooth muscle in rats; The chemical constituents of Heishunpian total alkaloids were analyzed by HPLC.
  
  
  RESULTS
  Heishunpian total alkaloids could significantly slow down intestine propulsive motility in diarrhea mice (P < 0. 05), and reduce amplitude and tension of isolated small intestinal smooth muscle contraction in normal rats in dose-dependent manner. Heishunpian total alkaloids had a certain inhibitory effect on acetylcholine chloride and neostigmine methylsulfate strengthening small intestine smooth muscle movement, however, had no significantly effect on Atropine sulfate inhibiting small intestine smooth muscle movement. By HPLC analysis, benzoylaconitine, benzoylmesaconitine, benzoylhypaconitine were the main chemical constituents of Heishunpian total alkaloids.
  
  
  CONCLUSIONS
  Heishunpian total alkaloids have a certain role of antidiarrheal and its mechanism may be related to antagonizing muscarinic receptors.
authors: Feng
authors: F
authors: Shao
authors: Hui-juan
authors: HJ
authors: Chen
authors: Rong-hua
authors: RH
authors: Liu
authors: Yong-chun
authors: YC
authors: Hou
authors: Gang
authors: G
authors: Ren
authors: Hui-lian
authors: HL
authors: Huang
authors: Fang-rui
authors: FR
authors: Tang
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000930:Antidiarrheals / Q000494:pharmacology*; D003967:Diarrhea / Q000139:chemically induced / Q000188:drug therapy*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004339:Drug Compounding; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D007421:Intestine, Small / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009119:Muscle Contraction / Q000187:drug effects* / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012676:Senna Extract / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D000930:Antidiarrheals; D004365:Drugs, Chinese Herbal; D012676:Senna Extract