title: Immunization with the recombinant Cholera toxin B fused to Fimbria 2 protein protects against Bordetella pertussis infection.
abstract: This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)-cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence. The expression and assembly of the fusion protein into pentameric structures (CTB-Fim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50 μg of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (P < 0.01 or P < 0.001 , resp.). Moreover, intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage (BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in a mixed Th1/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigen against Bordetella pertussis infection.
authors: Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-La Plata, CONICET-UNLP, Calle 49 y 115, 1900 La Plata, Argentina.
authors: Noelia
authors: 0000-0001-9492-6485
authors: N
authors: Olivera
authors: Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-La Plata, CONICET-UNLP, Calle 49 y 115, 1900 La Plata, Argentina.
authors: Celina E
authors: CE
authors: Castuma
authors: Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-La Plata, CONICET-UNLP, Calle 49 y 115, 1900 La Plata, Argentina.
authors: Daniela
authors: D
authors: Hozbor
authors: Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-La Plata, CONICET-UNLP, Calle 49 y 115, 1900 La Plata, Argentina.
authors: María E
authors: ME
authors: Gaillard
authors: LISIN, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 48 y 115, 1900 La Plata, Argentina.
authors: Martín
authors: M
authors: Rumbo
authors: Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-La Plata, CONICET-UNLP, Calle 49 y 115, 1900 La Plata, Argentina.
authors: Ricardo M
authors: RM
authors: Gómez
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid; D002772:Cholera Toxin / Q000276:immunology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D036781:Fimbriae Proteins / Q000276:immunology*; D056724:Immunity, Humoral; D007114:Immunization; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008168:Lung / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology* / Q000302:isolation & purification; D010566:Virulence Factors, Bordetella / Q000276:immunology*; D014917:Whooping Cough / Q000097:blood / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control*
chemical_list: D000942:Antigens, Bacterial; D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D010566:Virulence Factors, Bordetella; C101541:fim2 protein, Bordetella; D036781:Fimbriae Proteins; D002772:Cholera Toxin