title: Post-translational modification and extended glycosylation pattern of a plant latex peroxidase of native source characterized by X-ray crystallography.
abstract: The crystal structure of banyan peroxidase purified from the latex of Ficus benghalensis has been solved at 1.67 Å resolution by single-wavelength anomalous diffraction phasing. The refined structure includes 306 amino acid residues, a heme and two calcium ions. The protein belongs to class III peroxidases and is the first one from plant latex. Extensive glycosylation was observed with N-linked glycans attached to seven asparagine residues. The enzyme is stable with respect to a wide pH range, temperature, chemical denaturants and organic solvents, probably as a result of its high glycosylation. An unexpected post-translational modification of Asp290 was identified as succinimide moiety. Kinetic parameters of banyan peroxidase have been determined using various hydrogen donor substrates and hydrogen peroxide.
  
  
  METHODS
  Coordinates and structure factors have been deposited in the Protein Data Bank under accession number 4CUO.
authors: Institut für Biochemie, Universität Greifswald, Germany.
authors: Gottfried J
authors: GJ
authors: Palm
authors: Anurag
authors: A
authors: Sharma
authors: Moni
authors: M
authors: Kumari
authors: Santosh
authors: S
authors: Panjikar
authors: Dirk
authors: D
authors: Albrecht
authors: Medicherla V
authors: MV
authors: Jagannadham
authors: Winfried
authors: W
authors: Hinrichs
mesh_terms: D000595:Amino Acid Sequence; D002118:Calcium / Q000737:chemistry; D020134:Catalytic Domain; D056831:Coordination Complexes / Q000737:chemistry; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry; D004795:Enzyme Stability; D030681:Ficus / Q000201:enzymology*; D006031:Glycosylation; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D013379:Substrate Specificity
chemical_list: D056831:Coordination Complexes; D004791:Enzyme Inhibitors; D010940:Plant Proteins; D010544:Peroxidases; D002118:Calcium
keywords: glycosylation; plant latex; plant peroxidase; stability; succinimide