title: To achieve an earlier IFN-γ response is not sufficient to control Mycobacterium tuberculosis infection in mice.
abstract: The temporo-spatial relationship between the three organs (lung, spleen and lymph node) involved during the initial stages of Mycobacterium tuberculosis infection has been poorly studied. As such, we performed an experimental study to evaluate the bacillary load in each organ after aerosol or intravenous infection and developed a mathematical approach using the data obtained in order to extract conclusions. The results showed that higher bacillary doses result in an earlier IFN-γ response, that a certain bacillary load (BL) needs to be reached to trigger the IFN-γ response, and that control of the BL is not immediate after onset of the IFN-γ response, which might be a consequence of the spatial dimension. This study may have an important impact when it comes to designing new vaccine candidates as it suggests that triggering an earlier IFN-γ response might not guarantee good infection control, and therefore that additional properties should be considered for these candidates.
authors: Unitat de Tuberculosi Experimental (UTE), Fundació Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Universitat Autònoma de Barcelona, CIBERES, Badalona, Catalonia, Spain.
authors: Cristina
authors: C
authors: Vilaplana
authors: Escola Superior d'Agricultura de Barcelona, Departament de Física i Enginyeria Nuclear, Universitat Politècnica de Catalunya, C/Esteve Terradas, Castelldefels, Catalonia, Spain.
authors: Clara
authors: C
authors: Prats
authors: Unitat de Tuberculosi Experimental (UTE), Fundació Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Universitat Autònoma de Barcelona, CIBERES, Badalona, Catalonia, Spain.
authors: Elena
authors: E
authors: Marzo
authors: Escola Superior d'Agricultura de Barcelona, Departament de Física i Enginyeria Nuclear, Universitat Politècnica de Catalunya, C/Esteve Terradas, Castelldefels, Catalonia, Spain.
authors: Carles
authors: C
authors: Barril
authors: Escola Superior d'Agricultura de Barcelona, Departament de Física i Enginyeria Nuclear, Universitat Politècnica de Catalunya, C/Esteve Terradas, Castelldefels, Catalonia, Spain.
authors: Marina
authors: M
authors: Vegué
authors: Unitat de Tuberculosi Experimental (UTE), Fundació Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Universitat Autònoma de Barcelona, CIBERES, Badalona, Catalonia, Spain.
authors: Jorge
authors: J
authors: Diaz
authors: Escola Superior d'Agricultura de Barcelona, Departament de Física i Enginyeria Nuclear, Universitat Politècnica de Catalunya, C/Esteve Terradas, Castelldefels, Catalonia, Spain.
authors: Joaquim
authors: J
authors: Valls
authors: Escola Superior d'Agricultura de Barcelona, Departament de Física i Enginyeria Nuclear, Universitat Politècnica de Catalunya, C/Esteve Terradas, Castelldefels, Catalonia, Spain.
authors: Daniel
authors: D
authors: López
authors: Unitat de Tuberculosi Experimental (UTE), Fundació Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Universitat Autònoma de Barcelona, CIBERES, Badalona, Catalonia, Spain.
authors: Pere-Joan
authors: PJ
authors: Cardona
mesh_terms: D000818:Animals; D058491:Bacterial Load / Q000276:immunology; D005260:Female; D007371:Interferon-gamma / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018448:Models, Immunological; D009164:Mycobacterium Infections / Q000276:immunology* / Q000517:prevention & control; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000472:pathogenicity
chemical_list: D007371:Interferon-gamma