title: Mir-126 inhibits growth of SGC-7901 cells by synergistically targeting the oncogenes PI3KR2 and Crk, and the tumor suppressor PLK2.
abstract: MicroRNA (miRNA)-126 (miR-126) was reported to be downregulated and to act as a tumor suppressor in cancers of the lung, cervix, bladder and prostate. However, the functions of miR-126 in gastric cancer appear to be diverse and are largely unknown. MiR-126 was reported to act as a tumor suppressor by targeting the Crk gene, or as an oncogene by targeting the SOX2 gene in gastric cancer. We identified that the expression of miR-126 was decreased in gastric cancer cell lines and tissues. PLK2, a tumor suppressor gene, was directly regulated by miR-126 in SGC-7901 cells. Overexpression of miR-126 not only suppressed the growth and clone formation of SGC-7901 cells, but also induced apoptosis in vitro, whereas inhibition of miR-126 slightly promoted SGC-7901 cell proliferation. The cell cycle was not affected by miR-126. Moreover, miR-126 suppressed tumor growth in vivo in a xenograft model. PLK2, PI3KR2 and Crk were regulated by miR-126 in SGC-7901 cells. We infer that the functions of miR-126 in gastric cancer depend on synergistic targeting balance between oncogenes and anti-oncogenes. Our study indicates that miR-126 is a tumor suppressor, which in the future may become a therapeutic target for gastric cancer.
authors: The Center Laboratory for Biomedical Research, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710004, P.R. China.
authors: Li Ying
authors: LY
authors: Liu
authors: Department of Orthopaedics, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.
authors: Wei
authors: W
authors: Wang
authors: Department of Genetics and Cell Biology, Molecular Bacteriology Laboratory, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors: Lin Yu
authors: LY
authors: Zhao
authors: Department of Genetics and Cell Biology, Molecular Bacteriology Laboratory, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors: Bo
authors: B
authors: Guo
authors: Department of Genetics and Cell Biology, Molecular Bacteriology Laboratory, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors: Juan
authors: J
authors: Yang
authors: The Center Laboratory for Biomedical Research, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710004, P.R. China.
authors: Xiao Ge
authors: XG
authors: Zhao
authors: Department of Genetics and Cell Biology, Molecular Bacteriology Laboratory, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors: Ni
authors: N
authors: Hou
authors: Department of Genetics and Cell Biology, Molecular Bacteriology Laboratory, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors: Lei
authors: L
authors: Ni
authors: Department of Genetics and Cell Biology, Molecular Bacteriology Laboratory, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors: Ai Ying
authors: AY
authors: Wang
authors: Department of Genetics and Cell Biology, Molecular Bacteriology Laboratory, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors: Tu Sheng
authors: TS
authors: Song
authors: The Center Laboratory for Biomedical Research, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710004, P.R. China.
authors: Chen
authors: C
authors: Huang
authors: Department of Immunology and Pathogenic Biology, Molecular Bacteriology Laboratory, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors: Ji Ru
authors: JR
authors: Xu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D058543:Class Ia Phosphatidylinositol 3-Kinase / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009374:Neoplasms, Experimental; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D050737:Proto-Oncogene Proteins c-crk / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D050737:Proto-Oncogene Proteins c-crk; D058543:Class Ia Phosphatidylinositol 3-Kinase; C496126:PLK2 protein, human; D017346:Protein Serine-Threonine Kinases