title: Molecular evidence for piroplasms in wild Reeves' muntjac (Muntiacus reevesi) in China.
abstract: DNA from liver samples of 17 free-ranging wild Reeves' muntjac (Muntiacus reevesi) was used for PCR amplification of piropalsm 18S rRNA gene. Of 17 samples, 14 (82.4%) showed a specific PCR product which were cloned and sequenced. BLAST analysis of the sequences obtained showed similarities to Babesia sp., Theileria capreoli, Theileria uilenbergi and Theileria sp. BO302-SE. Phylogenetic analysis showed that the Babesia sp. detected in the present study was distantly separated from known Babesia species of wild and domestic animals. Six sequences showed 100% similarity to T. capreoli while five sequences were separated from all known Theileria species and constituted an independent clade with Theileria sp. BO302-SE derived from roe deer in Italy; two sequences were close to T. uilenbergi with 97% similarity. This is the first description of hemoparasite infection in free-ranging wild Reeves' muntjac in China. Our results indicate that wild Reeves' muntjac may play an important reservoir role for hemoparasites.
authors: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, PR China.
authors: Ji-fei
authors: JF
authors: Yang
authors: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, PR China.
authors: You-quan
authors: YQ
authors: Li
authors: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, PR China.
authors: Zhi-jie
authors: ZJ
authors: Liu
authors: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, PR China.
authors: Jun-long
authors: JL
authors: Liu
authors: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, PR China.
authors: Gui-quan
authors: GQ
authors: Guan
authors: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, PR China.
authors: Ze
authors: Z
authors: Chen
authors: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, PR China.
authors: Jian-xun
authors: JX
authors: Luo
authors: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, PR China; Center of Conservation Medicine & Ecological Safety, Northeast Forestry University, 26 Xiangfang District, Harbin, 150040, Heilongjiang, PR China. Electronic address: yttuhh@yeah.net.
authors: Xiao-long
authors: XL
authors: Wang
authors: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, PR China. Electronic address: yinhong@caas.net.cn.
authors: Hong
authors: H
authors: Yin
mesh_terms: D000818:Animals; D000835:Animals, Wild; D001404:Babesiosis / Q000453:epidemiology / Q000469:parasitology*; D002681:China / Q000453:epidemiology; D016054:DNA, Protozoan / Q000235:genetics; D003670:Deer; D010802:Phylogeny; D016792:Piroplasmida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D016054:DNA, Protozoan
keywords: 18S rRNA gene; Babesia; Phylogeny; Reeves' muntjac; Theileria