title: Stimulation of breast cancer cell lines by post-surgical drainage fluids.
abstract: OBJECTIVE
  Surgery, which remains a conventional treatment of breast tumors, may induce the secretion of growth factors that support angiogenesis and wound healing. These factors are suspected to trigger carcinoma cell division and promote tumor relapse. We addressed this question by culturing breast cancer cell lines in the presence of wound fluid harvested after surgery.
  
  
  METHODS
  Wound fluids were collected from patients who underwent either breast reconstruction, tumor resection, or tumor resection after neoadjuvant chemotherapy. MCF-7 (estrogen receptor (ER)+/progesterone receptor (PgR)+, HCC1937 (ER/PgR-, human epidermal growth factor receptor/neuralized (HER2/neu)-) and MCF-10A (used as a negative control) cell lines were grown in culture media supplemented with wound fluids.
  
  
  RESULTS
  Wound fluids drained during the three categories of procedures significantly stimulated the proliferation of MCF-7 and HCC1937 cells in a similar manner.
  
  
  CONCLUSIONS
  This stimulatory effect on tumor cell proliferation could be attenuated by therapeutic targeting against growth factors and inflammation processes in order to avoid tumor relapse.
authors: Laboratory for Tumour Biology, EA3430 of the University of Strasbourg, Strasbourg, France.
authors: Ludivine
authors: L
authors: Ramolu
authors: Department of Surgical Oncology, Paul Strauss Cancer Centre, Strasbourg, France.
authors: Dominique
authors: D
authors: Christ
authors: Laboratory for Tumour Biology, EA3430 of the University of Strasbourg, Strasbourg, France JAbecassis@strasbourg.unicancer.fr.
authors: Joseph
authors: J
authors: Abecassis
authors: Department of Surgical Oncology, Paul Strauss Cancer Centre, Strasbourg, France.
authors: Jean-François
authors: JF
authors: Rodier
mesh_terms: D001826:Body Fluids; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology* / Q000601:surgery*; D045744:Cell Line, Tumor; D017024:Chemotherapy, Adjuvant; D003470:Culture Media; D004322:Drainage; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D020360:Neoadjuvant Therapy; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D064726:Triple Negative Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D014945:Wound Healing / Q000502:physiology
chemical_list: D003470:Culture Media; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C508053:ERBB2 protein, human
keywords: Breast tumor; cell proliferation; drainage wound fluid; surgery