title: Development of an improved RT-LAMP assay for detection of currently circulating rubella viruses.
abstract: Rubella virus is the causative agent of rubella. The symptoms are usually mild, and characterized by a maculopapular rash and fever. However, rubella infection in pregnant women sometimes can result in the birth of infants with congenital rubella syndrome (CRS). Global efforts have been made to reduce and eliminate CRS. Although a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for detection of rubella virus has been reported, the primers contained several mismatched nucleotides with the genomes of currently circulating rubella virus strains. In the present study, a new RT-LAMP assay was established. The detection limit of this assay was 100-1000PFU/reaction of viruses for all rubella genotypes, except for genotype 2C, which is not commonly found in the current era. Therefore, the new RT-LAMP assay can successfully detect all current rubella virus genotypes, and does not require sophisticated devices like TaqMan real-time PCR systems. This assay should be a useful assay for laboratory diagnosis of rubella and CRS.
authors: Laboratory of Rubella, Department of Virology III, National Institute of Infectious Diseases, Murayama Branch, 4-7-1 Gakuen, Musashimurayama, 208-0011, Tokyo, Japan.
authors: H
authors: H
authors: Abo
authors: Laboratory of Rubella, Department of Virology III, National Institute of Infectious Diseases, Murayama Branch, 4-7-1 Gakuen, Musashimurayama, 208-0011, Tokyo, Japan. Electronic address: k-okmt@nih.go.jp.
authors: K
authors: K
authors: Okamoto
authors: Laboratory of Rubella, Department of Virology III, National Institute of Infectious Diseases, Murayama Branch, 4-7-1 Gakuen, Musashimurayama, 208-0011, Tokyo, Japan.
authors: M
authors: M
authors: Anraku
authors: Laboratory of Rubella, Department of Virology III, National Institute of Infectious Diseases, Murayama Branch, 4-7-1 Gakuen, Musashimurayama, 208-0011, Tokyo, Japan.
authors: N
authors: N
authors: Otsuki
authors: Laboratory of Rubella, Department of Virology III, National Institute of Infectious Diseases, Murayama Branch, 4-7-1 Gakuen, Musashimurayama, 208-0011, Tokyo, Japan.
authors: M
authors: M
authors: Sakata
authors: Measles, Mumps, Rubella and Herpes Virus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.
authors: J
authors: J
authors: Icenogle
authors: Measles, Mumps, Rubella and Herpes Virus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.
authors: Q
authors: Q
authors: Zheng
authors: Virology Division, Department of Infectious Diseases, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi, 1-chome, Higashinari-ku, Osaka 537-0025, Japan.
authors: T
authors: T
authors: Kurata
authors: Virology Division, Department of Infectious Diseases, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi, 1-chome, Higashinari-ku, Osaka 537-0025, Japan.
authors: T
authors: T
authors: Kase
authors: Laboratory of Rubella, Department of Virology III, National Institute of Infectious Diseases, Murayama Branch, 4-7-1 Gakuen, Musashimurayama, 208-0011, Tokyo, Japan.
authors: K
authors: K
authors: Komase
authors: Laboratory of Rubella, Department of Virology III, National Institute of Infectious Diseases, Murayama Branch, 4-7-1 Gakuen, Musashimurayama, 208-0011, Tokyo, Japan.
authors: M
authors: M
authors: Takeda
authors: Laboratory of Rubella, Department of Virology III, National Institute of Infectious Diseases, Murayama Branch, 4-7-1 Gakuen, Musashimurayama, 208-0011, Tokyo, Japan.
authors: Y
authors: Y
authors: Mori
mesh_terms: D005260:Female; D006801:Humans; D007231:Infant, Newborn; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011247:Pregnancy; D012409:Rubella / Q000175:diagnosis* / Q000821:virology*; D012412:Rubella virus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
keywords: Diagnosis; RT-LAMP; Rubella