title: Plasminogen activator inhibitor-1 and susceptibility to lung cancer: a population genetics perspective.
abstract: OBJECTIVE
  The aim of this study was to investigate the polymorphism frequency of plasminogen activator inhibitor-1 (PAI-1) (rs1799889) 4G/5G in patients with lung cancer.
  
  
  METHODS
  In this study, 286 genomic DNAs (154 lung cancer patients+132 subjects without lung cancer) were analyzed. Polymorphisms were determined by using the polymerase chain reaction (PCR) method, with 4G and 5G allele-specific primers. PCR products were assessed by a charge-coupled device camera and exposed to 2% agarose gel electrophoresis.
  
  
  RESULTS
  The frequencies of the PAI-1 gene 4G/5G genotypes were found to be 21% 4G/4G, 16% 4G/5G, and 62% 5G/5G in the control group and 31.4% 4G/4G, 30.8% 4G/5G, and 37.8% 5G/5G in the patient group. It was determined that the 5G/5G genotype frequency was high in patients in comparison with other genotypes.
  
  
  CONCLUSIONS
  This study found a statistically significant difference between the groups with respect to genotype distribution. Consequently, we can say that the PAI-1 gene 4G/5G polymorphism is associated with lung cancer in Turkey.
authors: 1 Department of Biology, Science and Art Faculty, Artvin Coruh University , Artvin, Turkey .
authors: Aysegul
authors: A
authors: Bayramoglu
authors: Hasan Veysi
authors: HV
authors: Gunes
authors: Muzaffer
authors: M
authors: Metintas
authors: Irfan
authors: I
authors: Degirmenci
authors: Halil Ibrahim
authors: HI
authors: Guler
authors: Cengiz
authors: C
authors: Ustuner
authors: Ahmet
authors: A
authors: Musmul
mesh_terms: D000483:Alleles; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics*; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D011110:Polymorphism, Genetic; D014421:Turkey / Q000453:epidemiology
chemical_list: D009363:Neoplasm Proteins; D017395:Plasminogen Activator Inhibitor 1; C051321:SERPINE1 protein, human