title: cRGD-conjugated magnetic-fluorescent liposomes for targeted dual-modality imaging of bone metastasis from prostate cancer.
abstract: We reported the development of multifunctional liposomes as a dual-modality probe to facilitate targeted magnetic resonance and fluorescent imaging of bone metastasis from advanced cancer. Multifunctional liposomes consisted of liposomes as a carrier, hydrophobic CdSe QDs in phospholipid bilayer, hydrophilic iron oxide nanoparticles in interior vesicle, lipid-PEG derivative on the surface and cRGDyk peptide conjugated to distal ends of lipid-PEG derivative. Excellent stability, effective detection signal, low toxicity, high resistance to phagocytosis by macrophages and good specificity to tumor of multifunctional liposomes were confirmed by in vitro characterization. The in vivo results demonstrated that multifunctional liposomes accumulated mainly in tumor and liver, indicating that targeted dual-modality imaging was achieved, and the results from two kinds of modalities were consistent and complementary. These findings provide a helpful strategy for detection of bone metastases in a more effective manner for initiation of appropriate therapy.
authors: Institute for Nautical Medicine, Nantong University , Nantong , People's Republic of China.
authors: Fangfang
authors: F
authors: Wang
authors: Zhongping
authors: Z
authors: Chen
authors: Li
authors: L
authors: Zhu
mesh_terms: D001859:Bone Neoplasms / Q000175:diagnosis* / Q000556:secondary; D019187:Cadmium Compounds / Q000737:chemistry; D003952:Diagnostic Imaging; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008297:Male; D009842:Oligopeptides / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000175:diagnosis*; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D008081:Liposomes; D058185:Magnetite Nanoparticles; D009842:Oligopeptides; D018036:Selenium Compounds; C047981:arginyl-glycyl-aspartic acid; C058667:cadmium selenide
keywords: Bone metastasis; RGD peptide; dual-modality imaging; iron oxide nanoparticles; liposome; quantum dots