title: Nanoscale cellular imaging with scanning angle interference microscopy.
abstract: Fluorescence microscopy is among the most widely utilized tools in cell and molecular biology due to its ability to noninvasively obtain time-resolved images of live cells with molecule-specific contrast. In this chapter, we describe a simple high-resolution technique, scanning angle interference microscopy (SAIM), for the imaging and localization of fluorescent molecules with nanometer precision along the optical axis. In SAIM, samples above a reflective surface are sequentially scanned with an excitation laser at varying angles of incidence. Interference patterns generated between the incident and reflected lights result in an emission intensity that depends on the height of a fluorophore above the silicon surface and the angle of the incident radiation. The measured fluorescence intensities are then fit to an optical model to localize the labeled molecules along the z-axis with 5-10 nm precision and diffraction-limited lateral resolution. SAIM is easily implemented on widely available commercial total internal reflection fluorescence microscopes, offering potential for widespread use in cell biology. Here, we describe the setup of SAIM and its application for imaging cellular structures near (<1 μm) the sample substrate.
authors: Department of Surgery, University of California, San Francisco, California, USA; Department of Orthopaedic Surgery, University of California, San Francisco, California, USA.
authors: Christopher
authors: C
authors: DuFort
authors: School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, USA; Kavli Institute at Cornell for Nanoscale Science, Ithaca, New York, USA.
authors: Matthew
authors: M
authors: Paszek
mesh_terms: D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry; D022001:Focal Adhesions / Q000648:ultrastructure; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D008857:Microscopy, Interference / Q000379:methods; D036103:Nanotechnology; D059010:Single-Cell Analysis / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes
keywords: Cytoskeleton; Dynamic; Focal adhesion; Interference; Membrane; Microscopy; Nanoscale; Superresolution