title: Comparative Evaluation of the Effect of EDTA, EDTAC, NaOCl and MTAD on Microhardness of Human Dentin - An In-vitro Study.
abstract: OBJECTIVE
  The aim of this study was to evaluate the microhardness of human dentin by using four irrigating solutions. Materials and Methodos: A total of 40 extracted mandibular premolars were selected and sectioned horizontally in the middle third of the root. Forty specimens of 4 mm thickness were embedded in acrylic resin and polished. Four test groups, each group containing ten specimens were immersed in respective irrigating solution and subjected to vicker's microhardness test at T0, T2 and T5min.
  
  
  METHODS
  The data obtained were analyzed using the one way ANOVA followed by Tukey HSD method with ap=0.05 as the level for statistical significance.
  
  
  RESULTS
  Suggested that there was no statistically significant difference in mean values between four experimental irrigating solutions.
  
  
  CONCLUSIONS
  Mixture of Tetracycline isomer i.e. Doxycycline, Citric acid and a Detergent (Tween 80) MTAD not altered the microhardness of root canal dentin significantly and seems to be an appropriate irrigating solution, because of its harmless effect on the microhardness of the root canal dentin.
authors: Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India .
authors: Rama S
authors: RS
authors: Kalluru
authors: Senior Lecturer, Department of Conservative Dentistry and Endodontics, RVS College of Dental Sciences , Coimbatore, Tamil Nadu, India .
authors: N Deepak
authors: ND
authors: Kumar
authors: Professor, Department of Conservative Dentistry and Endodontics, Raja Muthaiah Dental College , Chidambaram, Tamil Nadu, India .
authors: Shafie
authors: S
authors: Ahmed
authors: Professor, Department of Conservative Dentistry and Endodontics, Raja Muthaiah Dental College , Chidambaram, Tamil Nadu, India .
authors: Emanuel Solomon
authors: ES
authors: Sathish
authors: Professor, Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India .
authors: Thumu
authors: T
authors: Jayaprakash
authors: Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India .
authors: Roopadevi
authors: R
authors: Garlapati
authors: Lecturer, Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India .
authors: Butti
authors: B
authors: Sowmya
authors: Reader, Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India .
authors: K Narasimha
authors: KN
authors: Reddy
keywords: Irrigating solutions; Microhardness; Root canal dentin