title: Phosphoglucomutase1 is necessary for sustained cell growth under repetitive glucose depletion.
abstract: Phosphoglucomutase (PGM)1 catalyzes the reversible conversion reaction between glucose-1-phosphate (G-1-P) and glucose-6-phosphate (G-6-P). Although both G-1-P and G-6-P are important intermediates for glucose and glycogen metabolism, the biological roles and regulatory mechanisms of PGM1 are largely unknown. In this study we found that T553 is obligatory for PGM1 stability and the last C-terminal residue, T562, is critical for its activity. Interestingly, depletion of PGM1 was associated with declined cellular glycogen content and decreased rates of glycogenolysis and glycogenesis. Furthermore, PGM1 depletion suppressed cell proliferation under long-term repetitive glucose depletion. Our results suggest that PGM1 is required for sustained cell growth during nutritional changes, probably through regulating the balance of G-1-P and G-6-P in order to satisfy the cellular demands during nutritional stress.
authors: Department of Biochemistry and Molecular Biology, Brain Korea 21 PLUS Project for Medical Sciences, Institute of Genetic Science, Integrated Genomic Research Center for Metabolic Regulation, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors: Eunju
authors: E
authors: Bae
authors: Department of Biochemistry and Molecular Biology, Brain Korea 21 PLUS Project for Medical Sciences, Institute of Genetic Science, Integrated Genomic Research Center for Metabolic Regulation, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors: Hee Eun
authors: HE
authors: Kim
authors: Department of Biochemistry and Molecular Biology, Brain Korea 21 PLUS Project for Medical Sciences, Institute of Genetic Science, Integrated Genomic Research Center for Metabolic Regulation, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea. Electronic address: KOHCHUN@yuhs.ac.
authors: Eunjin
authors: E
authors: Koh
authors: Department of Biochemistry and Molecular Biology, Brain Korea 21 PLUS Project for Medical Sciences, Institute of Genetic Science, Integrated Genomic Research Center for Metabolic Regulation, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea. Electronic address: KYUNGSUP59@yuhs.ac.
authors: Kyung-Sup
authors: KS
authors: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004795:Enzyme Stability; D005947:Glucose / Q000172:deficiency* / Q000494:pharmacology; D019298:Glucose-6-Phosphate / Q000378:metabolism; D005958:Glucosephosphates / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D010733:Phosphoglucomutase / Q000737:chemistry / Q000378:metabolism*; D013912:Threonine
chemical_list: D005958:Glucosephosphates; D013912:Threonine; D019298:Glucose-6-Phosphate; D006003:Glycogen; C031590:glucose-1-phosphate; D010733:Phosphoglucomutase; D005947:Glucose
keywords: Glycogen; Glycogenesis; Glycogenolysis; Nutritional stress; Phosphoglucomutase