title: The chick chorioallantoic membrane: a model of short-term study of Dupuytren's disease.
abstract: Dupuytren's disease is a progressive fibroproliferative disorder that impairs hand function by altering the normal structures of the palmar fascial bands. Nodules composed almost entirely of myofibroblasts and cords are pathognomonic of Dupuytren's disease. The myofibroblasts express alpha-smooth muscle actin that is especially involved in development of the disease. We aimed to evaluate whether the xenograft of Dupuytren's fibromatosis taken from operating room and transplanted on chick chorioallantoic membrane (CAM) survives with its histological and immunohistological features. Fresh samples obtained from eight patients with Dupuytren's disease were minced and immediately inoculated onto 24 CAMs of 8-day-old chick embryos. The implanted CAMs were examined daily by stereomicroscopy and finally the xenografts were examined and characterized in histological sections using a panel of antibodies. The xenografts were incorporated into the CAMs 6-7 days after transplantation, continued to grow and stimulated angiogenesis in the chick embryo CAMs. The CAMs vessels entered the xenografts and anastomosed with the newly formed xenografts vessels (CD34+ and CD105+) those containing nucleated chick erythrocytes. Myofibroblasts (α-SMA+) and macrophages (CD68+) were readily recognized in the xenograft thickness. We concluded that the xenografts of Dupuytren's fibromatosis transplanted onto chick embryo CAMs continued to develop and preserved the histological and immunohistological features.
authors: Department of Morphological Sciences, University of Medicine and Pharmacy of Craiova, Romania; tutu0101@yahoo.com.
authors: Ion
authors: I
authors: Mîndrilă
authors: Horia
authors: H
authors: Pârvănescu
authors: Daniel
authors: D
authors: Pirici
authors: Mihaela
authors: M
authors: Niculescu
authors: Sandra Alice
authors: SA
authors: Buteică
authors: Oana
authors: O
authors: Taisescu
authors: Dănut Nicolae
authors: DN
authors: Tarnita
mesh_terms: D000818:Animals; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000473:pathology*; D004387:Dupuytren Contracture / Q000473:pathology*; D005205:Fascia / Q000098:blood supply / Q000473:pathology / Q000637:transplantation; D064593:Heterografts; D006801:Humans; D023421:Models, Animal; D058628:Myofibroblasts / Q000473:pathology / Q000637:transplantation; D013997:Time Factors