title: Development of a cell-based assay for the detection of anti-aquaporin 1 antibodies in neuromyelitis optica spectrum disorders.
abstract: OBJECTIVE
  To develop a cell-based assay (CBA) to detect aquaporin 1 (AQP1) antibodies and determine sensitivity/specificity in patients with neuromyelitis optica (NMO) spectrum disorders.
  
  
  METHODS
  A HEK-293T transfected cell model expressing AQP1 was established and detected to be serum AQP1 antibodies.
  
  
  RESULTS
  AQP1 antibodies were present in 73/98 (74.5%) AQP4 antibody-positive patients. Some AQP4 antibody-negative patients were also AQP1 antibody-positive. Test sensitivity was 74.5% in 98 AQP4 antibody-positive patients. Test specificity was 79.6% in 67 multiple sclerosis (MS) patients and 31 controls.
  
  
  CONCLUSIONS
  A sensitive and simple CBA was developed to detect serum AQP1 antibodies. AQP1 antibodies were mainly present in NMO and its high-risk syndrome, but also in some MS patients.
authors: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China.
authors: Youming
authors: Y
authors: Long
authors: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China.
authors: Yangbo
authors: Y
authors: Zheng
authors: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China.
authors: Fulan
authors: F
authors: Shan
authors: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China.
authors: Mengyu
authors: M
authors: Chen
authors: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China.
authors: Yongxiang
authors: Y
authors: Fan
authors: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China.
authors: Bin
authors: B
authors: Zhang
authors: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China. Electronic address: smilegaocong@126.com.
authors: Cong
authors: C
authors: Gao
authors: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China.
authors: Qingchun
authors: Q
authors: Gao
authors: Department of Neurology, The Fifth Affiliated Hospital of GuangZhou Medical University, 621# Gangwan Road, GuangZhou, 510700 Guangdong Province, China.
authors: Ning
authors: N
authors: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000097:blood; D051398:Aquaporin 1 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051401:Aquaporin 4 / Q000276:immunology; D001921:Brain / Q000473:pathology; D002648:Child; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D009471:Neuromyelitis Optica / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D014162:Transfection; D055815:Young Adult
chemical_list: D000906:Antibodies; D051401:Aquaporin 4; D051398:Aquaporin 1
keywords: Aquaporin 1 antibody; Cell-based assay; Multiple sclerosis; Neuromyelitis optica