title: Evaluation of selected cytokine gene expression in colonic mucosa from dogs with idiopathic lymphocytic-plasmacytic colitis.
abstract: Lymphocytic-plasmacytic colitis (LPC) is a common form of inflammatory bowel disease (IBD) affecting the canine large intestine. Cytokines are thought to be involved in the pathogenesis of IBD. However, to date, few studies have investigated cytokine mRNA expression in dogs with LPC. In this study, we investigated mRNA transcription levels of T helper cell cytokines, such as IFN-γ, IL-4, IL-17 and IL-10 and pro-inflammatory cytokines, such as IL-1β, IL-6, TNF-α, IL-8, IL-12 and IL-23, in colonic mucosa from LPC dogs by quantitative real-time RT-PCR. No significant differences were detected in cytokine mRNA expressions between dogs with LPC and controls, except for IL-23p19. Dogs with LPC failed to express a predominant cytokine profile in inflamed colonic mucosa as opposed to human IBD.
authors: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors: Yu
authors: Y
authors: Tamura
authors: Hiroshi
authors: H
authors: Ohta
authors: Nozomu
authors: N
authors: Yokoyama
authors: Sue Yee
authors: SY
authors: Lim
authors: Tatsuyuki
authors: T
authors: Osuga
authors: Keitaro
authors: K
authors: Morishita
authors: Kensuke
authors: K
authors: Nakamura
authors: Masahiro
authors: M
authors: Yamasaki
authors: Mitsuyoshi
authors: M
authors: Takiguchi
mesh_terms: D000818:Animals; D003092:Colitis / Q000145:classification / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D003106:Colon / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male
chemical_list: D016207:Cytokines