title: Interplay between polymorphisms in the endothelial nitric oxide synthase (eNOS) gene and metabolic syndrome in determining the risk of ischemic stroke in Koreans.
abstract: BACKGROUND
  Endothelial nitric oxide synthase (eNOS) gene variants are known to play a role in atherosclerotic development. However, whether interplay between eNOS polymorphisms and metabolic syndrome (MetS) affects ischemic stroke (IS) risk has yet to be discovered. We investigated whether the combined effects of eNOS polymorphisms and MetS influence ischemic stroke risk in Koreans.
  
  
  METHODS
  We genotyped the eNOS -922A>G, -786T>C, 4a4b, and 894G>T polymorphisms in 531 IS cases and 502 controls using polymerase chain reaction-amplified DNA. We then investigated whether the presence of MetS and the number of MetS risk factors worked with eNOS polymorphisms to influence IS risk.
  
  
  RESULTS
  IS patients had a significantly higher prevalence of MetS than controls [adjusted odds ratio (AOR)=2.943, 95% confidence interval (CI), 2.256-3.840, P<0.0001], and MetS prevalence did not differ between stroke subtypes. The 894GT+TT genotypes were positively associated with IS (AOR=1.670, 95% CI, 1.208-2.308, P=0.002), and the -786TC+CC genotypes showed co-morbidity with MetS (AOR=1.448, 95% CI, 1.401-2.015, P=0.028). Among subjects with three or more MetS risk factors, the highest AOR value (28.490, 95% CI, 3.162-256.688) was observed for the eNOS -786TC+CC genotypes.
  
  
  CONCLUSIONS
  The eNOS 894T allele and interplay between the eNOS -786C allele and MetS may predispose Koreans to IS.
authors: Department of Biomedical Science, College of Life Science, CHA University, Republic of Korea.
authors: Min Kyu
authors: MK
authors: Kang
authors: Department of Neurology, School of Medicine, CHA University, Seongnam, Republic of Korea.
authors: Ok Joon
authors: OJ
authors: Kim
authors: The Institute for Clinical Research, School of Medicine, CHA University, Seongnam, Republic of Korea.
authors: Young Joo
authors: YJ
authors: Jeon
authors: Department of Neurology, School of Medicine, CHA University, Seongnam, Republic of Korea.
authors: Hyun Sook
authors: HS
authors: Kim
authors: Department of Neurology, School of Medicine, CHA University, Seongnam, Republic of Korea.
authors: Seung Hun
authors: SH
authors: Oh
authors: Department of Neurology, School of Medicine, CHA University, Seongnam, Republic of Korea.
authors: Jin Kwon
authors: JK
authors: Kim
authors: Department of Medicine, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.
authors: Eo Jin
authors: EJ
authors: Kim
authors: Department of Anatomy, School of Medicine, CHA University, Seongnam, Republic of Korea. Electronic address: tshwang@cha.ac.kr.
authors: Tae Sun
authors: TS
authors: Hwang
authors: Department of Biomedical Science, College of Life Science, CHA University, Republic of Korea; The Institute for Clinical Research, School of Medicine, CHA University, Seongnam, Republic of Korea. Electronic address: nkkim@cha.ac.kr.
authors: Nam Keun
authors: NK
authors: Kim
mesh_terms: D000368:Aged; D000886:Anthropometry; D015992:Body Mass Index; D002545:Brain Ischemia / Q000150:complications; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D008297:Male; D008659:Metabolic Diseases / Q000209:etiology*; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D056910:Republic of Korea; D012307:Risk Factors; D020521:Stroke / Q000150:complications* / Q000209:etiology / Q000235:genetics*
chemical_list: D052250:Nitric Oxide Synthase Type III
keywords: Endothelial nitric oxide synthase (eNOS); Ischemic stroke; Metabolic syndrome; Nitric oxide (NO); Polymorphism