title: [Preparation of novel gypenosides long-circulating liposomes consisted by sphingomyelin and beta-sitosterol modified by PEG].
abstract: OBJECTIVE
  To explore the feasibility of preparing novel gypenosides long-circulating liposomes with PEG grafted on beta-sitosterol (PEG-Sito).
  
  
  METHODS
  Succinicanhydride was adopted to connect beta-sitosterol and PEG 2000. Sphingomyelin and PEG-Sito was used as material to prepare gypenosides long-circulating liposomes by using ethanol injection method. Encapsulation efficiency was determined by using protamine precipitation method. H-NMR was used to verify the synthesis of PEG-Sito, the novel gypenosides long-circulating liposomes were characterized by particle size, zeta potential and atomic force microscope.
  
  
  RESULTS
  The synthesis of PEG-Sito was verified by 1H-NMR. Encapsulation efficiency of long-circulating liposomes prepared by ethanol injection method was 74.3%, particle size was 288.1 nm, zeta potential was -20.25 mV, the morphology were round observed by AFM.
  
  
  CONCLUSIONS
  The novel gypenosides long-circulating liposomes prepared with PEG-Sito was feasible, it had a high encapsulation efficiency and good morphology.
authors: Fan
authors: F
authors: Yu
authors: Jing-Ming
authors: JM
authors: Yang
authors: Jin-Juan
authors: JJ
authors: Li
mesh_terms: D004339:Drug Compounding / Q000379:methods*; D005240:Feasibility Studies; D029774:Gynostemma / Q000737:chemistry; D008081:Liposomes / Q000097:blood* / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D015203:Reproducibility of Results; D012855:Sitosterols / Q000737:chemistry*; D013109:Sphingomyelins / Q000737:chemistry*
chemical_list: D008081:Liposomes; D010936:Plant Extracts; D012855:Sitosterols; D013109:Sphingomyelins; C474918:gypenoside; D011092:Polyethylene Glycols; C025473:gamma-sitosterol