title: MSRB7 reverses oxidation of GSTF2/3 to confer tolerance of Arabidopsis thaliana to oxidative stress.
abstract: Methionine sulfoxide reductases (MSRs) catalyse the reduction of oxidized methionine residues, thereby protecting proteins against oxidative stress. Accordingly, MSRs have been associated with stress responses, disease, and senescence in a taxonomically diverse array of organisms. However, the cytosolic substrates of MSRs in plants remain largely unknown. Here, we used a proteomic analysis strategy to identify MSRB7 substrates. We showed that two glutathione transferases (GSTs), GSTF2 and GSTF3, had fewer oxidized methionine (MetO) residues in MSRB7-overexpressing Arabidopsis thaliana plants than in wild-type plants. Conversely, GSTF2 and GSTF3 were highly oxidized and unstable in MSRB7-knockdown plants. MSRB7 was able to restore the MetO-GSTF2M100/104 and MetO-GSTF3M100 residues produced during oxidative stress. Furthermore, both GSTs were specifically induced by the oxidative stress inducer, methyl viologen. Our results indicate that specific GSTs are substrates of MSRs, which together provide a major line of defence against oxidative stress in A. thaliana.
authors: Institute of Biotechnology, National Cheng Kung University, Tainan, 701, Taiwan Academia Sinica Biotechnology Center in Southern Taiwan, Tainan, 741, Taiwan Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan.
authors: Shu-Hong
authors: SH
authors: Lee
authors: Department of Biotechnology, TransWorld University, Douliu City, Yunlin County, 640, Taiwan.
authors: Chia-Wen
authors: CW
authors: Li
authors: Academia Sinica Biotechnology Center in Southern Taiwan, Tainan, 741, Taiwan Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan.
authors: Kah Wee
authors: KW
authors: Koh
authors: Academia Sinica Biotechnology Center in Southern Taiwan, Tainan, 741, Taiwan Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan.
authors: Hsin-Yu
authors: HY
authors: Chuang
authors: Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan.
authors: Yet-Ran
authors: YR
authors: Chen
authors: Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan.
authors: Choun-Sea
authors: CS
authors: Lin
authors: Institute of Biotechnology, National Cheng Kung University, Tainan, 701, Taiwan Academia Sinica Biotechnology Center in Southern Taiwan, Tainan, 741, Taiwan Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan mbmtchan@gate.sinica.edu.tw.
authors: Ming-Tsair
authors: MT
authors: Chan
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D051150:Methionine Sulfoxide Reductases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D029681:Arabidopsis Proteins; D051150:Methionine Sulfoxide Reductases; C580622:MsrB7 protein, Arabidopsis; C478066:GSTF2 protein, Arabidopsis; D005982:Glutathione Transferase
keywords: A. thaliana; LC-MS/MS; glutathione transferase; methionine sulfoxide reductase B (MSRB).; methyl viologen; oxidative stress