title: Catalase addition to vitrification solutions maintains goat ovarian preantral follicles stability.
abstract: The aim of this study was to verify whether the addition of catalase (20 IU/mL) at different steps of goat ovarian tissue vitrification affects ROS levels, follicular morphology and viability, stromal cell density, apoptosis and the expression of proteins related to DNA-damage signaling (γH2AX) and repair (53BP1). Goat ovarian tissues were analyzed fresh (control) or after vitrification: without catalase (VS-/WS-), with catalase in vitrification solutions (VS+/WS-), with catalase in washing solutions (VS-/WS+) or with catalase in both solutions (VS+/WS+). The vitrification without catalase had higher ROS levels than the control. The catalase, regardless the step of addition, maintained ROS levels similar to the control. There were no difference between treatments regarding follicular viability, stromal cell density and detection of γH2AX and 53BP1. There was no difference in follicular morphology and DNA fragmentation between groups vitrified. In conclusion, catalase addition to vitrification solutions prevents ROS formation in cryopreserved goat ovarian tissues.
authors: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
authors: A A
authors: AA
authors: Carvalho
authors: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
authors: L R
authors: LR
authors: Faustino
authors: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
authors: C M G
authors: CM
authors: Silva
authors: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
authors: S V
authors: SV
authors: Castro
authors: Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, CE, Brazil.
authors: C H
authors: CH
authors: Lobo
authors: Laboratory of Reproduction Biotechnology (BIOTECH), Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
authors: F W
authors: FW
authors: Santos
authors: Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands; Animal Science Post-graduation Program, Federal University of Pará, Belém, PA, Brazil.
authors: R R
authors: RR
authors: Santos
authors: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
authors: C C
authors: CC
authors: Campello
authors: Department of Animal Science, McGill University, Ste-Anne-de-Bellevue, QC, Canada.
authors: V
authors: V
authors: Bordignon
authors: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
authors: J R
authors: JR
authors: Figueiredo
authors: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil. Electronic address: aprrodriguespapers@gmail.com.
authors: A P R
authors: AP
authors: Rodrigues
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005260:Female; D006041:Goats; D006657:Histones / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects*; D010750:Phosphoproteins / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D058989:Vitrification
chemical_list: D006657:Histones; D010750:Phosphoproteins; D017382:Reactive Oxygen Species; D002374:Catalase
keywords: Apoptosis; Cryopreservation; DNA damage; Ovary; ROS levels