title: Mulberry fruit extract protects pancreatic β-cells against hydrogen peroxide-induced apoptosis via antioxidative activity.
abstract: Among the many environmental stresses, excessive production of reactive oxygen species (ROS) and the ensuring oxidative stress are known to cause significant cellular damage. This has clinical implications in the onset of type 1 diabetes, which is triggered by the destruction of pancreatic β-cells and is associated with oxidative stress. In this study, we investigated the protective and antioxidative effects of mulberry extract (ME) in insulin-producing pancreatic β-cells. We found that ME protects pancreatic β-cells against hydrogen peroxide (H2O2)-induced oxidative stress and the associated apoptotic cell death. ME treatment significantly reduced the levels of H2O2-induced 2-diphenyl-1-picrylhydrazyl (DPPH) radicals, and lipid peroxidation and intracellular ROS accumulation. In addition, ME inhibited DNA condensation and/or fragmentation induced by H2O2. These results suggest that ME protects pancreatic β-cells against hydrogen peroxide-induced oxidative stress.
authors: Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea. jongseoklee78@gmail.com.
authors: Jong Seok
authors: JS
authors: Lee
authors: Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea. youngraekim0709@gmail.com.
authors: Young Rae
authors: YR
authors: Kim
authors: Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea. three0313@nate.com.
authors: Jun Myoung
authors: JM
authors: Park
authors: Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea. sjha@kangwon.ac.kr.
authors: Suk-Jin
authors: SJ
authors: Ha
authors: Korea Food Research Institute, Seongnam 463746, Korea. radog@kfri.re.kr.
authors: Young Eon
authors: YE
authors: Kim
authors: Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Youngin 446701, Korea. nibaek@khu.ac.kr.
authors: Nam In
authors: NI
authors: Baek
authors: Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea. ekhong@kangwon.ac.kr.
authors: Eock Kee
authors: EK
authors: Hong
mesh_terms: D000818:Animals; D017209:Apoptosis; D001713:Biphenyl Compounds / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D031625:Morus / Q000737:chemistry*; D018384:Oxidative Stress; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl