title: Enzyme orientation for direct electron transfer in an enzymatic fuel cell with alcohol oxidase and laccase electrodes.
abstract: A new full enzymatic fuel cell was built and characterized. Both enzymatic electrodes were molecularly oriented to enhance the direct electron transfer between the enzyme active site and the electrode surface. The anode consisted in immobilized alcohol oxidase on functionalized carbon nanotubes with 4-azidoaniline, which acts as active-site ligand to orientate the enzyme molecule. The cathode consisted of immobilized laccase on functionalized graphite electrode with 4-(2-aminoethyl) benzoic acid. The enzymatic fuel cell reaches 0.5 V at open circuit voltage with both, ethanol and methanol, while in short circuit the highest current intensity of 250 μA cm(-2) was obtained with methanol. Concerning the power density, the methanol was the best substrate reaching 60 μW cm(-2), while with ethanol 40 μW cm(-2) was obtained.
authors: Instituto de Biotecnologia UNAM, Cuernavaca, Morelos, Mexico.
authors: Andrés A
authors: AA
authors: Arrocha
authors: Instituto de Investigaciones Eléctricas, Cuernavaca, Morelos, Mexico.
authors: Ulises
authors: U
authors: Cano-Castillo
authors: Centro de Nanociencias y Nanotecnología UNAM, Ensenada, Baja California, Mexico.
authors: Sergio A
authors: SA
authors: Aguila
authors: Instituto de Biotecnologia UNAM, Cuernavaca, Morelos, Mexico; Centro de Nanociencias y Nanotecnología UNAM, Ensenada, Baja California, Mexico. Electronic address: rvd@cnyn.unam.mx.
authors: Rafael
authors: R
authors: Vazquez-Duhalt
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D001487:Basidiomycota / Q000201:enzymology*; D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques / Q000295:instrumentation*; D004566:Electrodes; D004579:Electron Transport; D004583:Electrons; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D042845:Laccase / Q000737:chemistry*; D010843:Pichia / Q000201:enzymology*
chemical_list: D004800:Enzymes, Immobilized; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D005949:Glucose Oxidase; D042845:Laccase
keywords: Alcohol oxidase; Enzymatic electrodes; Fuel cell; Laccase; Molecular orientation