title: Viable mononuclear cell stability study for implementation in a proficiency testing program: impact of shipment conditions.
abstract: The impact of shipping temperatures and preservation media used during transport of either peripheral blood mononuclear cells (PBMCs) or Jurkat cells was assessed, in view of implementing of a proficiency testing scheme on mononuclear cell viability. Samples were analyzed before and after shipment at different temperatures (ambient temperature, dry ice, and liquid nitrogen) and in different preservation media (serum with cryoprotectant, commercial cryopreservation solution, and room temperature transport medium). Sample quality was assessed by viability assays (Trypan Blue dye exclusion, flow cytometry, Cell Analysis System cell counting (CASY)), and by ELISpot functional assay. The liquid nitrogen storage and shipment were found to be the most stable conditions to preserve cell viability and functionality. However, we show that alternative high quality shipment conditions for viable cells are dry ice shipment and commercial cryopreservation solution. These were also cost-efficient shipment conditions, satisfying the requirements of a proficiency testing scheme for viable mononuclear cells. Room temperature transport medium dramatically and adversely affected the integrity of mononuclear cells.
authors: 1 Integrated BioBank Of Luxembourg (IBBL) , 6 rue Nicolas Ernest Barblé, L-1210, Luxembourg .
authors: Olga A
authors: OA
authors: Kofanova
authors: Kristine
authors: K
authors: Davis
authors: Barbara
authors: B
authors: Glazer
authors: Yvonne
authors: Y
authors: De Souza
authors: Joseph
authors: J
authors: Kessler
authors: Fotini
authors: F
authors: Betsou
mesh_terms: D001793:Blood Preservation / Q000379:methods*; D001800:Blood Specimen Collection / Q000379:methods*; D002470:Cell Survival; D003451:Cryoprotective Agents / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D007963:Leukocytes, Mononuclear / Q000166:cytology; D009584:Nitrogen / Q000494:pharmacology*; D011786:Quality Control; D013696:Temperature
chemical_list: D003451:Cryoprotective Agents; D009584:Nitrogen