title: Assessment of antioxidative, chelating, and DNA-protective effects of selected essential oil components (eugenol, carvacrol, thymol, borneol, eucalyptol) of plants and intact Rosmarinus officinalis oil.
abstract: Selected components of plant essential oils and intact Rosmarinus officinalis oil (RO) were investigated for their antioxidant, iron-chelating, and DNA-protective effects. Antioxidant activities were assessed using four different techniques. DNA-protective effects on human hepatoma HepG2 cells and plasmid DNA were evaluated with the help of the comet assay and the DNA topology test, respectively. It was observed that whereas eugenol, carvacrol, and thymol showed high antioxidative effectiveness in all assays used, RO manifested only antiradical effect and borneol and eucalyptol did not express antioxidant activity at all. DNA-protective ability against hydrogen peroxide (H2O2)-induced DNA lesions was manifested by two antioxidants (carvacrol and thymol) and two compounds that do not show antioxidant effects (RO and borneol). Borneol was able to preserve not only DNA of HepG2 cells but also plasmid DNA against Fe(2+)-induced damage. This paper evaluates the results in the light of experiences of other scientists.
authors: Department of Genetics, Cancer Research Institute, Slovak Academy of Sciences, Vlarska 7, 833 91 Bratislava, Slovak Republic.
authors: Eva
authors: E
authors: Horvathova
authors: Jana
authors: J
authors: Navarova
authors: Eliska
authors: E
authors: Galova
authors: Andrea
authors: A
authors: Sevcovicova
authors: Lenka
authors: L
authors: Chodakova
authors: Zuzana
authors: Z
authors: Snahnicanova
authors: Martina
authors: M
authors: Melusova
authors: Katarina
authors: K
authors: Kozics
authors: Darina
authors: D
authors: Slamenova
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001892:Camphanes / Q000494:pharmacology; D002614:Chelating Agents / Q000032:analysis*; D003511:Cyclohexanols / Q000494:pharmacology; D000081007:Cymenes; D004249:DNA Damage / Q000187:drug effects*; D000077591:Eucalyptol; D005054:Eugenol / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D039821:Monoterpenes / Q000494:pharmacology; D009822:Oils, Volatile / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D027542:Rosmarinus / Q000737:chemistry*; D013943:Thymol / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001892:Camphanes; D002614:Chelating Agents; D003511:Cyclohexanols; D000081007:Cymenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010938:Plant Oils; D013943:Thymol; D005054:Eugenol; C073316:carvacrol; D006861:Hydrogen Peroxide; C022871:isoborneol; D000077591:Eucalyptol