title: Liquid chromatography-mass spectrometry analysis of diethylcarbamazine in human plasma for clinical pharmacokinetic studies.
abstract: A sensitive and selective liquid chromatographic method using mass spectrometric detection was developed for the determination of diethylcarbamazine (DEC) in human plasma. DEC and its stable isotope internal standard d3-DEC were extracted from 0.25mL of human plasma using solid phase extraction. Chromatography was performed using a Phenomenex Synergi 4μ Fusion-RP column (2mm×250mm) with gradient elution. The retention time was approximately 4.8min. The assay was linear from 4 to 2200ng/mL. Analysis of quality control samples at 12, 300, and 1700ng/mL (N=15) had interday coefficients of variation of 8.4%, 5.4%, and 6.2%, respectively (N=15). Interday bias results were -2.2%, 6.0%, and 0.8%, respectively. Recovery of DEC from plasma ranged from 84.2% to 90.1%. The method was successfully applied to clinical samples from patients with lymphatic filariasis from a drug-drug interaction study between DEC and albendazole and/or ivermectin.
authors: College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA.
authors: Mark S
authors: MS
authors: Schmidt
authors: School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.
authors: Christopher L
authors: CL
authors: King
authors: Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.
authors: Edward K
authors: EK
authors: Thomsen
authors: Papua New Guinea Institute for Medical Research, Papua New Guinea.
authors: Peter M
authors: PM
authors: Siba
authors: Papua New Guinea Institute for Medical Research, Papua New Guinea.
authors: Nelly
authors: N
authors: Sanuku
authors: College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA. Electronic address: l-fleckenstein@uiowa.edu.
authors: Lawrence
authors: L
authors: Fleckenstein
mesh_terms: D015766:Albendazole / Q000097:blood / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D004049:Diethylcarbamazine / Q000097:blood* / Q000737:chemistry*; D004605:Elephantiasis, Filarial / Q000097:blood; D006801:Humans; D007559:Ivermectin / Q000097:blood / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D010949:Plasma / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D007559:Ivermectin; D015766:Albendazole; D004049:Diethylcarbamazine
keywords: Antifilarial; Diethylcarbamazine; Liquid chromatography–mass spectrometry; Plasma