title: [Quantitative detection of matrix metalloproteinase-2 in normal coronal dentine of young people].
abstract: OBJECTIVE
  To compare the distribution and concentration of matrix metalloproteinases-2 (MMP-2) in different dentin depth of premolar and molar of young people.
  
  
  METHODS
  Freshly extracted human premolars and molars (aged between 20-30) were sectioned to 1.5 mm thick slices along the longitudinal axis of the tooth separately. Enamel and pulp of each slice was removed, and then the premolar and molar slices were respectively divided into two subgroups according to superficial or deep dentin and pulverized to fine powder. After dentin protein was extracted, the concentrations of MMP-2 in different tooth were detected using fluorescent microsphere immunoassay.
  
  
  RESULTS
  The content of MMP-2 in superficial layer dentin of premolar was (0.022 ± 0.006) ng/mg. The content of MMP-2 in deep layer dentin of premolar was (2.087 ± 0.090) ng/mg. The content of MMP-2 in superficial layer dentin of molar was (0.336 ± 0.037) ng/mg. The content of MMP-2 in deep layer dentin of molar was (3.312 ± 0.308) ng/mg.
  
  
  CONCLUSIONS
  MMP-2 exists in human coronal dentin. In the same type of teeth of young people, the concentration of MMP-2 in deep dentin was significant higher than those in superficial dentin. In the same dentin depth, the concentration of MMP-2 in molar was significant higher than those in premolar.
authors: Department of Prosthodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors: Danyang
authors: D
authors: Wang
authors: Email: zhang.ling1978@gmail.com.
authors: Ling
authors: L
authors: Zhang
authors: Fang
authors: F
authors: Li
authors: Yujiang
authors: Y
authors: Chen
authors: Jihua
authors: J
authors: Chen
mesh_terms: D000328:Adult; D001641:Bicuspid; D003743:Dental Enamel; D003782:Dental Pulp; D003804:Dentin / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000032:analysis*; D008963:Molar; D014070:Tooth; D019228:Tooth Crown; D014092:Tooth Root; D055815:Young Adult
chemical_list: D020778:Matrix Metalloproteinase 2