title: The viral envelope is not sufficient to transfer the unique broad cell tropism of Bungowannah virus to a related pestivirus.
abstract: Bungowannah virus is the most divergent pestivirus, and both origin and reservoir host have not been identified so far. We therefore performed in vitro tropism studies, which showed that Bungowannah virus differs remarkably from other pestiviruses. Interestingly, cell lines of vervet monkey, mouse, human and even of bat origin were susceptible. This broad in vitro tropism was not observed for a chimeric bovine viral diarrhoea virus (BVDV) expressing all structural proteins of Bungowannah virus. The viral envelope was not sufficient to completely transfer the cell tropism of Bungowannah virus to another pestivirus, and viral RNA replication was either markedly reduced or not detectable in a number of different cell lines for the tested BVDV strain and the chimera. We therefore suggest that the replication machinery together with the viral envelope is responsible for the unique broad cell tropism of Bungowannah virus.
authors: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, D-17493 Greifswald-Insel Riems, Germany.
authors: Maria
authors: M
authors: Richter
authors: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, D-17493 Greifswald-Insel Riems, Germany.
authors: Ilona
authors: I
authors: Reimann
authors: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, D-17493 Greifswald-Insel Riems, Germany.
authors: Horst
authors: H
authors: Schirrmeier
authors: Virology Laboratory, Elizabeth Macarthur Agriculture Institute, New South Wales Department of Primary Industries, Narellan, NSW 2567, Australia.
authors: Peter D
authors: PD
authors: Kirkland
authors: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, D-17493 Greifswald-Insel Riems, Germany.
authors: Martin
authors: M
authors: Beer
mesh_terms: D000818:Animals; D002460:Cell Line; D002685:Chiroptera; D002522:Chlorocebus aethiops; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000502:physiology; D006801:Humans; D051379:Mice; D010576:Pestivirus / Q000235:genetics / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D056189:Viral Tropism
chemical_list: D014759:Viral Envelope Proteins