title: Phenotypic features of first-generation transgenic goats for human granulocyte-colony stimulation factor production in milk.
abstract: Human granulocyte-colony stimulating factor (hG-CSF) is a hematopoietic growth factor used in neutropenic patients. It is produced in transgenic bacteria or cultured mammalian cells. As an alternative, we now show that hG-CSF can be expressed in the mammary gland of first-generation (F1) transgenic goats during induced lactation. Despite lower milk production, transgenic females presented a similar milk composition (fat, protein and lactose) when compared to non-transgenic (p > 0.05) ones. The mean concentration (±SD) of recombinant hG-CSF in milk during lactation was 360 ± 178 µg ml(-1). All clinical parameters, as well as kidney and liver function, indicated that F1 transgenic goats were healthy. Additionally, no ectopic hG-CSF expression was detected in studied tissues of F1 transgenic males. Thus, F1 hG-CSF-transgenic goats can express the recombinant protein in milk at quantities compatible with their use as bioreactors in a commercial-scale protein-production program.
authors: Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE, 60714-903, Brazil.
authors: Ribrio I T P
authors: RI
authors: Batista
authors: Carlos H S
authors: CH
authors: Melo
authors: Joanna M G
authors: JM
authors: Souza-Fabjan
authors: Dárcio I A
authors: DI
authors: Teixeira
authors: Luciana M
authors: LM
authors: Melo
authors: Vicente J F
authors: VJ
authors: Freitas
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019149:Bioreactors; D005260:Female; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007774:Lactation; D008297:Male; D008892:Milk / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor