title: Determination of β-N-oxalyl-L-α,β-diaminopropionic acid and homoarginine in Lathyrus sativus and Lathyrus cicera by capillary zone electrophoresis.
abstract: BACKGROUND
  Lathyrus species as legumes represent an alternative protein source for human and animal nutrition. Heavy consumption of these species can lead to lathyrism, caused by the non-protein amino acid β-N-oxalyl-l-α,β-diaminopropionic acid (β-ODAP). Currently, there is no well-defined level below which β-ODAP is considered non-toxic. In this work, the β-ODAP content was determined in L. sativus and L. cicera samples to assess their potential toxicity. Homoarginine is another non-protein amino acid found in Lathyrus spp. with interesting implications for human and animal nutrition.
  
  
  RESULTS
  The level of β-ODAP found in these two species ranged from 0.79 to 5.05 mg g(-1). The homoarginine content of the samples ranged from 7.49 to 12.44 mg g(-1).
  
  
  CONCLUSIONS
  This paper describes an accurate, fast and sensitive method of simultaneous detection and quantification of β-ODAP and homoarginine by capillary zone electrophoresis in L. cicera and L. sativus seeds. Moreover, several methods of extraction were compared to determine the highest performance.
authors: Dpto. Tecnología de Alimentos, SGIT-INIA, Apdo. 8111, 28080, Madrid, Spain.
authors: Mara
authors: M
authors: Sacristán
authors: Alejandro
authors: A
authors: Varela
authors: Mercedes M
authors: MM
authors: Pedrosa
authors: Carmen
authors: C
authors: Burbano
authors: Carmen
authors: C
authors: Cuadrado
authors: M Estrella
authors: ME
authors: Legaz
authors: Mercedes
authors: M
authors: Muzquiz
mesh_terms: D000818:Animals; D004032:Diet; D004044:Dietary Proteins; D019075:Electrophoresis, Capillary / Q000379:methods; D006709:Homoarginine / Q000009:adverse effects / Q000032:analysis*; D006801:Humans; D007842:Lathyrism / Q000209:etiology; D027807:Lathyrus / Q000009:adverse effects / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D015091:beta-Alanine / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D004044:Dietary Proteins; D015091:beta-Alanine; D006709:Homoarginine; C011757:2,3-diaminopropionic acid
keywords: Lathyrus cicera; Lathyrus sativus; capillary zone electrophoresis; homoarginine; neurolathyrism; β-ODAP