title: Spatiotemporal expression of Wnt5a during the development of the striated muscle complex in rats with anorectal malformations.
abstract: Fecal incontinence and constipation after procedures for anorectal malformations (ARMs) are closely related to the maldevelopment of the striated muscle complex (SMC). Previous studies have demonstrated that myogenic regulatory factors (MRFs) play a significant role in muscle development. Wnt signal pathway is extremely important for MRFs regulation. This study was designed to investigate the spatiotemporal expression pattern of Wnt5a in SMC in ARMs rat embryos.
  
  
  METHODS
  Anorectal malformation embryos were induced by ethylene thiourea on embryonic day 10 (E10). Expression levels of protein and mRNA of Wnt5a were confirmed by immunohistochemistry staining, western blot and quantitative real-time PCR (qRT-PCR) between normal rat embryos and embryos with ARMs.
  
  
  RESULTS
  Immunostaining revealed that, on embryonic day 17 (E17), the Wnt5a protein was initially expressed in the SMC in normal embryos. With the growth of pregnancy, the positive staining cells gradually increased. The same time-dependent changes of Wnt5a protein were detected in ARMs embryos. Besides, immunostaining showed that Wnt5a had a significant increase in normal embryos compared with ARMs embryos. Similarly, in Western blot and qRT-PCR, the higher expression of Wnt5a protein and mRNA were remarkable in normal embryos during the SMC development, relatively.
  
  
  CONCLUSIONS
  Our study demonstrated that the downregulation of Wnt5a at the time of SMC development might partly be related to the dysplasia of SMC in ARMs.
authors: Department of Pediatric Surgery, Shengjing Hospital of China Medical University Liaoning, China.
authors: Jie
authors: J
authors: Mi
authors: Department of Pediatric Surgery, Shengjing Hospital of China Medical University Liaoning, China.
authors: Dong
authors: D
authors: Chen
authors: Department of Pediatric Surgery, Shengjing Hospital of China Medical University Liaoning, China.
authors: Xiantan
authors: X
authors: Ren
authors: Department of Pediatric Surgery, Shengjing Hospital of China Medical University Liaoning, China.
authors: Huimin
authors: H
authors: Jia
authors: Key Laboratory of Congenital Malformations, Ministry of Public Health, Shengjing Hospital of China Medical University Shenyang, Liaoning, China.
authors: Hong
authors: H
authors: Gao
authors: Department of Pediatric Surgery, Shengjing Hospital of China Medical University Liaoning, China.
authors: Weilin
authors: W
authors: Wang
mesh_terms: D000818:Animals; D000071056:Anorectal Malformations; D001006:Anus, Imperforate / Q000139:chemically induced / Q000378:metabolism*; D015153:Blotting, Western; D004195:Disease Models, Animal; D005031:Ethylenethiourea; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D007150:Immunohistochemistry; D008297:Male; D018811:Maternal Exposure; D024510:Muscle Development; D054792:Muscle, Striated / Q000002:abnormalities / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*; D000071818:Wnt-5a Protein
chemical_list: D012333:RNA, Messenger; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C491837:Wnt5a protein, rat; D005031:Ethylenethiourea
keywords: Anorectal malformations; Wnt5a; expression; striated muscle complex