title: Experimental study on effect of simulated microgravity on structural chromosome instability of human peripheral blood lymphocytes.
abstract: Experimental study was made by keeping human peripheral blood lymphocytes under simulated microgravity in a Rotary Cell Culture System bioreactor to investigate the changes that occur in the number of chromosomes, the expression rate of chromosome fragile site, and the expressions of DNA replication- and repair-related genes. Experimental results indicate simulated microgravity has no effect on the numerical chromosome instability of human peripheral blood lymphocytes, but it enhances the structural chromosome instability of human peripheral blood lymphocytes through the inhibition of DNA replication and the reduction of DNA repair. So, the mechanism of chromosome fragile site induced by simulated microgravity can be explained using the changes that occur in the chromosome structure of human peripheral blood lymphocytes, the DNA replication and repair under the effect of simulated microgravity.
authors: School of Life Science and Technology, Harbin Institute of Technology, Harbin, China; State Key Laboratory of Space Medicine Fundamentals and Application, Chinese Astronaut Research and Training Center, Beijing, China.
authors: Lijun
authors: L
authors: Wei
authors: School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.
authors: Chuanpeng
authors: C
authors: Liu
authors: School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.
authors: Li
authors: L
authors: Kang
authors: School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.
authors: Yufeng
authors: Y
authors: Liu
authors: School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.
authors: Shuliang
authors: S
authors: Shi
authors: School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.
authors: Qiong
authors: Q
authors: Wu
authors: School of Life Science and Technology, Harbin Institute of Technology, Harbin, China; State Key Laboratory of Space Medicine Fundamentals and Application, Chinese Astronaut Research and Training Center, Beijing, China.
authors: Yu
authors: Y
authors: Li
mesh_terms: D064007:Ataxia Telangiectasia Mutated Proteins / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D043171:Chromosomal Instability; D003470:Culture Media / Q000737:chemistry; D004260:DNA Repair / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D018474:Weightlessness Simulation
chemical_list: D003470:Culture Media; C576597:ATR protein, human; D064007:Ataxia Telangiectasia Mutated Proteins