title: Biosynthesis of polybrominated aromatic organic compounds by marine bacteria.
abstract: Polybrominated diphenyl ethers (PBDEs) and polybrominated bipyrroles are natural products that bioaccumulate in the marine food chain. PBDEs have attracted widespread attention because of their persistence in the environment and potential toxicity to humans. However, the natural origins of PBDE biosynthesis are not known. Here we report marine bacteria as producers of PBDEs and establish a genetic and molecular foundation for their production that unifies paradigms for the elaboration of bromophenols and bromopyrroles abundant in marine biota. We provide biochemical evidence of marine brominases revealing decarboxylative-halogenation enzymology previously unknown among halogenating enzymes. Biosynthetic motifs discovered in our study were used to mine sequence databases to discover unrealized marine bacterial producers of organobromine compounds.
authors: 1] Center for Oceans and Human Health, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, California, USA. [2].
authors: Vinayak
authors: V
authors: Agarwal
authors: 1] Center for Oceans and Human Health, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, California, USA. [2].
authors: Abrahim A
authors: AA
authors: El Gamal
authors: 1] Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, California, USA. [2].
authors: Kazuya
authors: K
authors: Yamanaka
authors: Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, California, USA.
authors: Dennis
authors: D
authors: Poth
authors: 1] Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, California, USA. [2].
authors: Roland D
authors: RD
authors: Kersten
authors: Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, California, USA.
authors: Michelle
authors: M
authors: Schorn
authors: 1] Center for Oceans and Human Health, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, California, USA. [2] Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, California, USA. [3] Division of Biological Sciences, University of California-San Diego, La Jolla, California, USA.
authors: Eric E
authors: EE
authors: Allen
authors: 1] Center for Oceans and Human Health, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, California, USA. [2] Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, California, USA. [3] Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California-San Diego, La Jolla, California, USA.
authors: Bradley S
authors: BS
authors: Moore
mesh_terms: D059001:Aquatic Organisms; D016680:Genome, Bacterial; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism*; D054879:Halogenation; D008969:Molecular Sequence Data; D005810:Multigene Family; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D031161:Pseudoalteromonas / Q000235:genetics* / Q000378:metabolism*; D011758:Pyrroles / Q000378:metabolism
chemical_list: D055768:Halogenated Diphenyl Ethers; D011758:Pyrroles; D010088:Oxidoreductases