title: Basal transcription factor 3 plays an important role in seed germination and seedling growth of rice.
abstract: BTF3 has been recognized to be involved in plant growth and development. But its function remains mostly unknown during seed germination and seedling stage. Here, we have analyzed OsBTF3-related sequences in Oryza sativa L. subspecies, japonica, which resembles with the conserved domain of a nascent polypeptide associated complex (NAC) with different homologs of OsBTF3 and human BTF3. Inhibition of Osj10gBTF3 has led to considerable morphological changes during seed germination and seedling growth. Germination percentage was not influenced by the application of GA3, ABA, and NaCl but all concentrations caused wild-type (WT) seeds to germinate more rapidly than the RNAi (Osj10gBTF3 (Ri)) transgenic lines. Seedling inhibition was more severe in the Osj10gBTF3 (Ri) seedlings compared with their WT especially when treated with 100 or 200 μM GA3; 50% reduction in shoots was observed in Osj10gBTF3 (Ri) seedlings. The expression of Osj3g1BTF3, Osj3g2BTF3 and Osj10gBTF3 was primarily constitutive and generally modulated by NaCl, ABA, and GA3 stresses in both Osj10gBTF3 (Ri) lines and WT at the early seedling stage, suggesting that Osj3g1BTF3 and Osj10gBTF3 are much similar but different from Osj3g2BTF3 in biological function. These results show that OsBTF3 plays an important role in seed germination and seedling growth gives a new perception demonstrating that more multifaceted regulatory functions are linked with BTF3 in plants.
authors: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Yu-Hang-Tang Road 388, Hangzhou 310058, China.
authors: Wenyi
authors: W
authors: Wang
authors: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Yu-Hang-Tang Road 388, Hangzhou 310058, China.
authors: Mengyun
authors: M
authors: Xu
authors: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Yu-Hang-Tang Road 388, Hangzhou 310058, China.
authors: Ya
authors: Y
authors: Wang
authors: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Yu-Hang-Tang Road 388, Hangzhou 310058, China ; Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat 26000, Pakistan.
authors: Muhammad
authors: 0000-0002-2889-6676
authors: M
authors: Jamil
mesh_terms: D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D018525:Germination; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics; D018520:Plant Shoots; D030821:Plants, Genetically Modified / Q000235:genetics; D034622:RNA Interference; D036226:Seedlings / Q000235:genetics; D012639:Seeds / Q000254:growth & development; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D058127:Transcription Factor 3 / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D058127:Transcription Factor 3; D012965:Sodium Chloride