title: Synergistic apoptosis-inducing effects on A375 human melanoma cells of natural borneol and curcumin.
abstract: This study was to investigate the synergistic effect of NB/Cur on growth and apoptosis in A375 human melanoma cell line by MTT assay, flow cytometry and Western blotting. Our results demonstrated that NB effectively synergized with Cur to enhance its antiproliferative activity on A375 human melanoma cells by induction of apoptosis, as evidenced by an increase in sub-G1 cell population, DNA fragmentation, PARP cleavage and caspase activation. Further mechanistic studies by Western blotting showed that after treatment of the cells with NB/Cur, up-regulation of the expression level of phosphorylated JNK and down-regulation of the expression level of phosphorylated ERK and Akt contributed to A375 cells apoptosis. Moreover, NB also potentiated Cur to trigger intracellular ROS overproduction and the DNA damage with up-regulation of the expression level of phosphorylated ATM, phosphorylated Brca1 and phosphorylated p53. The results indicate the combinational application potential of NB and Cur in treatments of cancers.
authors: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
authors: Jianping
authors: J
authors: Chen
authors: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
authors: Lin
authors: L
authors: Li
authors: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China; Guangdong Hua Qing Yuan Biological Technology Co., Ltd., Meizhou, China.
authors: Jianyu
authors: J
authors: Su
authors: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
authors: Bing
authors: B
authors: Li
authors: Department of Chemistry, Jinan University, Guangzhou, China; Guangdong Hua Qing Yuan Biological Technology Co., Ltd., Meizhou, China.
authors: Tianfeng
authors: T
authors: Chen
authors: School of Life Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China.
authors: Yum-Shing
authors: YS
authors: Wong
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001892:Camphanes / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D020935:MAP Kinase Signaling System; D008545:Melanoma / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D001892:Camphanes; D017382:Reactive Oxygen Species; D051057:Proto-Oncogene Proteins c-akt; D048670:MAP Kinase Kinase 4; D003474:Curcumin; C022871:isoborneol