title: Convenient, sensitive and high-throughput method for screening botanic origin.
abstract: In this work, a rapid (within 4-5 h), sensitive and visible new method for assessing botanic origin is developed by combining loop-mediated isothermal amplification with cationic conjugated polymers. The two Chinese medicinal materials (Jin-Yin-Hua and Shan-Yin-Hua) with similar morphology and chemical composition were clearly distinguished by gene SNP genotyping assays. The identification of plant species in Patented Chinese drugs containing Lonicera buds is successfully performed using this detection system. The method is also robust enough to be used in high-throughput screening. This new method is very helpful to identify herbal materials, and is beneficial for detecting safety and quality of botanic products.
authors: 1] State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, P. R. China [2].
authors: Yuan
authors: Y
authors: Yuan
authors: 1] Beijing Key Laboratory of Protection and Application of Chinese Medicinal Resources, Beijing Normal University, Beijing 100875, P. R. China [2].
authors: Chao
authors: C
authors: Jiang
authors: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
authors: Libing
authors: L
authors: Liu
authors: State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, P. R. China.
authors: Shulin
authors: S
authors: Yu
authors: State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, P. R. China.
authors: Zhanhu
authors: Z
authors: Cui
authors: State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, P. R. China.
authors: Min
authors: M
authors: Chen
authors: State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, P. R. China.
authors: Shufang
authors: S
authors: Lin
authors: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
authors: Shu
authors: S
authors: Wang
authors: State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, P. R. China.
authors: Luqi
authors: L
authors: Huang
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods; D018744:DNA, Plant / Q000235:genetics*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000145:classification; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010946:Plants, Medicinal / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D018744:DNA, Plant; D004365:Drugs, Chinese Herbal