title: Pom1 regulates the assembly of Cdr2-Mid1 cortical nodes for robust spatial control of cytokinesis.
abstract: Proper division plane positioning is essential to achieve faithful DNA segregation and to control daughter cell size, positioning, or fate within tissues. In Schizosaccharomyces pombe, division plane positioning is controlled positively by export of the division plane positioning factor Mid1/anillin from the nucleus and negatively by the Pom1/DYRK (dual-specificity tyrosine-regulated kinase) gradients emanating from cell tips. Pom1 restricts to the cell middle cortical cytokinetic ring precursor nodes organized by the SAD-like kinase Cdr2 and Mid1/anillin through an unknown mechanism. In this study, we show that Pom1 modulates Cdr2 association with membranes by phosphorylation of a basic region cooperating with the lipid-binding KA-1 domain. Pom1 also inhibits Cdr2 interaction with Mid1, reducing its clustering ability, possibly by down-regulation of Cdr2 kinase activity. We propose that the dual regulation exerted by Pom1 on Cdr2 prevents Cdr2 assembly into stable nodes in the cell tip region where Pom1 concentration is high, which ensures proper positioning of cytokinetic ring precursors at the cell geometrical center and robust and accurate division plane positioning.
authors: Centre de Recherche and Laboratory of Mass Spectrometry and Proteomics, Institut Curie, F-75248 Paris, France Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, F-75248 Paris, France.
authors: Sergio A
authors: SA
authors: Rincon
authors: Department of Fundamental Microbiology, University of Lausanne, Lausanne CH-1015, Switzerland.
authors: Payal
authors: P
authors: Bhatia
authors: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK.
authors: Claudia
authors: C
authors: Bicho
authors: Centre de Recherche and Laboratory of Mass Spectrometry and Proteomics, Institut Curie, F-75248 Paris, France Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, F-75248 Paris, France.
authors: Mercè
authors: M
authors: Guzman-Vendrell
authors: Centre de Recherche and Laboratory of Mass Spectrometry and Proteomics, Institut Curie, F-75248 Paris, France Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, F-75248 Paris, France.
authors: Vincent
authors: V
authors: Fraisier
authors: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK.
authors: Weronika E
authors: WE
authors: Borek
authors: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK.
authors: Flavia de Lima
authors: Fde L
authors: Alves
authors: Centre de Recherche and Laboratory of Mass Spectrometry and Proteomics, Institut Curie, F-75248 Paris, FranceCentre de Recherche and Laboratory of Mass Spectrometry and Proteomics, Institut Curie, F-75248 Paris, France.
authors: Florent
authors: F
authors: Dingli
authors: Centre de Recherche and Laboratory of Mass Spectrometry and Proteomics, Institut Curie, F-75248 Paris, FranceCentre de Recherche and Laboratory of Mass Spectrometry and Proteomics, Institut Curie, F-75248 Paris, France.
authors: Damarys
authors: D
authors: Loew
authors: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK.
authors: Juri
authors: J
authors: Rappsilber
authors: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK.
authors: Kenneth E
authors: KE
authors: Sawin
authors: Department of Fundamental Microbiology, University of Lausanne, Lausanne CH-1015, Switzerland.
authors: Sophie G
authors: SG
authors: Martin
authors: Centre de Recherche and Laboratory of Mass Spectrometry and Proteomics, Institut Curie, F-75248 Paris, France Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, F-75248 Paris, France anne.paoletti@curie.fr.
authors: Anne
authors: A
authors: Paoletti
mesh_terms: D020090:Chromosome Segregation; D015825:Chromosomes, Fungal / Q000378:metabolism; D048749:Cytokinesis; D057927:Hydrophobic and Hydrophilic Interactions; D010766:Phosphorylation; D011485:Protein Binding; D011494:Protein Kinases / Q000502:physiology*; D055503:Protein Multimerization; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D012568:Schizosaccharomyces / Q000166:cytology* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: C489694:Mid1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; C112212:Pom1 protein, S pombe; C542567:Cdr2 protein, S pombe; D017346:Protein Serine-Threonine Kinases