title: Improvement of preimplantation development of in vitro-fertilized bovine zygotes by glucose supplementation to a chemically defined medium.
abstract: The influences of glucose supplementation on early development of bovine embryos in BSA-free synthetic oviduct fluid were examined. Among the groups supplemented with 1.5, 2.0, 4.0 or 5.6 mM glucose either at 0, 72 or 144 hr after fertilization, blastocysts yield significantly increased in the group supplemented with 4.0 mM glucose 144 hr after fertilization compared to the controls without glucose supplementation. The results suggest that appropriate amounts of glucose supplemented to the medium at the specific stage of embryo culture may be useful for the production of bovine blastocysts.
authors: Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa 243-0417, Japan.
authors: Nobutada
authors: N
authors: Sakagami
authors: Osamu
authors: O
authors: Nishino
authors: Satoshi
authors: S
authors: Adachi
authors: Hidenobu
authors: H
authors: Umeki
authors: Hiroko
authors: H
authors: Uchiyama
authors: Kyoko
authors: K
authors: Ichikawa
authors: Kazuhisa
authors: K
authors: Takeshita
authors: Etsushi
authors: E
authors: Kaneko
authors: Kiyoshi
authors: K
authors: Akiyama
authors: Shuji
authors: S
authors: Kobayashi
authors: Hiromichi
authors: H
authors: Tamada
mesh_terms: D000818:Animals; D002417:Cattle / Q000196:embryology*; D046149:Embryo Culture Techniques / Q000662:veterinary*; D047108:Embryonic Development / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000662:veterinary*; D005947:Glucose / Q000494:pharmacology*; D015053:Zygote / Q000187:drug effects* / Q000254:growth & development
chemical_list: D005947:Glucose