title: Esterases immobilized on aminosilane modified magnetic nanoparticles as a catalyst for biotransformation reactions.
abstract: Magnetite nanoparticles were prepared by reacting ferrous and ferric salts in presence of aqueous ammonia. The magnetic nanoparticles (MNPs) were amino functionalized by treating with 3-aminopropyl triethoxy silane (APTES) and was coupled with glutaraldehyde. A novel solvent tolerant esterase from Pseudozyma sp. NII 08165 was immobilized on the MNPs through covalent bonding to the glutaraldehyde. The magnetite nanoparticles had a size range of 10-100 nm, confirmed by DLS. Lipases immobilized on MNPs were evaluated for biotransformation reactions including synthesis of ethyl acetate and transesterification of vegetable oil for producing biodiesel. The MNP immobilized esterase had prolonged shelf life and there was no loss in enzyme activity.
authors: Centre for Biofuels, Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate PO, Thiruvananthapuram 695019, Kerala, India; Department of Biotechnology, Mar Ivanios College, Bethany Hills, Thiruvananthapuram 695015, Kerala, India.
authors: Deepthy
authors: D
authors: Alex
authors: Centre for Biofuels, Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate PO, Thiruvananthapuram 695019, Kerala, India; Department of Botany, St. Peter's College, Kolenchery, Ernakulam, Kerala, India.
authors: Abraham
authors: A
authors: Mathew
authors: Centre for Biofuels, Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate PO, Thiruvananthapuram 695019, Kerala, India. Electronic address: rajeevs@niist.res.in.
authors: Rajeev K
authors: RK
authors: Sukumaran
mesh_terms: D056804:Biofuels; D001711:Biotransformation; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004950:Esterases / Q000378:metabolism*; D004951:Esterification; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D012821:Silanes; D004950:Esterases
keywords: Biodiesel; Ethyl acetate; Lipase; Magnetic nanoparticles; Pseudozyma