title: Sulforaphane attenuates homocysteine-induced endoplasmic reticulum stress through Nrf-2-driven enzymes in immortalized human hepatocytes.
abstract: In the present study, we investigated the potential efficacy of cruciferous vegetable-derived sulforaphane (SFN) in improving homocysteine (HCY)-stressed cells. After human hepatocyte line HHL-5 was preincubated with SFN and subsequently with 10 mmol/L HCY, SFN improved the pathologic changes which are caused by HCY, including cell morphological abnormality, endoplasmic reticulum (ER) swelling, excessive generation of reactive oxygen species (ROS), the increased malondialdehyde (MDA) levels, as well as the increased activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Phase II enzymes, thioredoxin reductase-1 (TrxR-1) and
  quinone oxidoreductase 1 (NQO1), were involved in the protective effect of SFN against injury by HCY. The ER stress-specific proteins, such as glucose-regulated protein-78 (GRP78) and protein kinase RNA (PKR)-like ER kinase (PERK), were strikingly abolished by SFN. Furthermore, Nrf-2 translocation was enhanced by SFN, which lead to the induction of TrxR-1and NQO1.
authors: School of Food Science and Engineering, Harbin Institute of Technology , No. 73 Huanghe Road, Harbin 150090, China.
authors: Canxia
authors: C
authors: He
authors: Baolong
authors: B
authors: Li
authors: Wei
authors: W
authors: Song
authors: Zhongqing
authors: Z
authors: Ding
authors: Shuran
authors: S
authors: Wang
authors: Yujuan
authors: Y
authors: Shan
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology; D006710:Homocysteine / Q000008:administration & dosage / Q000009:adverse effects*; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013454:Sulfoxides; D054481:Thioredoxin Reductase 1 / Q000378:metabolism
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D017879:Isothiocyanates; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D017382:Reactive Oxygen Species; D013454:Sulfoxides; D006710:Homocysteine; D008315:Malondialdehyde; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human; C516124:TXNRD1 protein, human; D054481:Thioredoxin Reductase 1; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C016766:sulforaphane