title: Bone formation with deproteinized bovine bone mineral or biphasic calcium phosphate in the presence of autologous platelet lysate: comparative investigation in rabbit.
abstract: Bone substitutes alone or supplemented with platelet-derived concentrates are widely used to promote bone regeneration but their potency remains controversial. The aim of this study was, therefore, to compare the regenerative potential of preparations containing autologous platelet lysate (APL) and particles of either deproteinized bovine bone mineral (DBBM) or biphasic calcium phosphate (BCP), two bone substitutes with different resorption patterns. Rabbit APL was prepared by freeze-thawing a platelet suspension. Critical-size defects in rabbit femoral condyle were filled with DBBM or DBBM+APL and BCP or BCP+APL. Rabbits were sacrificed after six weeks and newly formed bone and residual implanted material were evaluated using nondemineralized histology and histomorphometry. New bone was observed around particles of all fillers tested. In the defects filled with BCP, the newly formed bone area was greater (70%; P < 0.001) while the residual material area was lower (60%; P < 0.001) than that observed in those filled with DBBM. New bone and residual material area of defects filled with either APL+DBBM or APL+BCP were similar to those observed in those filled with the material alone. In summary, osteoconductivity and resorption of BCP were greater than those of DBBM, while APL associated with either DBBM or BCP did not have an additional benefit.
authors: Department of Periodontology, School of Dentistry, Saint-Joseph University, P.O. Box 11-5076, Riad el Solh, Beirut 1107 2050, Lebanon ; Laboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires, UMR CNRS 7052, PRES Sorbonne Paris Cité, 75010 Paris, France.
authors: Carole
authors: 0000-0001-9781-9941
authors: C
authors: Chakar
authors: Department of Periodontology, School of Dentistry, Saint-Joseph University, P.O. Box 11-5076, Riad el Solh, Beirut 1107 2050, Lebanon.
authors: Nada
authors: N
authors: Naaman
authors: Laboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires, UMR CNRS 7052, PRES Sorbonne Paris Cité, 75010 Paris, France ; Department of Prosthetic Dentistry, Service of Odontology, Pitié-Salpêtrière Hospital, AP-HP, U.F.R. d'Odontologie, Paris 7-Diderot University, Paris, France.
authors: Emmanuel
authors: E
authors: Soffer
authors: Laboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires, UMR CNRS 7052, PRES Sorbonne Paris Cité, 75010 Paris, France ; Department of Periodontology, Service of Odontology, Pitié-Salpêtrière Hospital, AP-HP, U.F.R. d'Odontologie, Paris 7-Diderot University, Paris, France.
authors: Nicolas
authors: N
authors: Cohen
authors: Department of Prosthetic Dentistry, School of Dentistry, Saint-Joseph University, P.O. Box 11-5076, Riad el Solh, Beirut 1107 2050, Lebanon.
authors: Nada
authors: N
authors: El Osta
authors: Laboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires, UMR CNRS 7052, PRES Sorbonne Paris Cité, 75010 Paris, France.
authors: Hervé
authors: H
authors: Petite
authors: Laboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires, UMR CNRS 7052, PRES Sorbonne Paris Cité, 75010 Paris, France ; Department of Periodontology, Service of Odontology, Pitié-Salpêtrière Hospital, AP-HP, U.F.R. d'Odontologie, Paris 7-Diderot University, Paris, France.
authors: Fani
authors: 0000-0002-0065-2127
authors: F
authors: Anagnostou