title: Allogenous skin fibroblast transplantation enhances excisional wound healing following alloxan diabetes in sheep, a randomized controlled trial.
abstract: BACKGROUND
  Healing of skin wound is a multi-factorial and complex process. Treatment of diabetic wounds is still a major clinical challenge. Recently, stem cell transplantation to chronic wounds is favored. The objective of this study was to evaluate effects of pre-labeled allogenous skin fibroblasts on healing of ovine diabetic wound model.
  
  
  METHODS
  Eight 4-month-old Iranian Makoui wethers were used in this study. Alloxan monohydrate was used for induction of diabetes. In each wether two excisional wound were created on dorsum of the animal. Wounds of one side were randomly chosen as treatment group (n = 8), and wounds of the other side were considered as control group (n = 8). Pre-labeled skin fibroblasts with bromodeoxyuridine were used in wounds of one side as treatment. Photographs were taken in distinct times for planimetric evaluation. Wound samples were taken for BrdU detection and histopathologic evaluations on day 21 post-wounding.
  
  
  RESULTS
  The planimetric study showed closure of fibroblast treated wounds is significantly faster than control group (P < 0.05). Immunohistochemical staining with anti-bromodeoxyuridine antibody indicated presence of transplanted cells in the wounds. Histopathologic evaluations of H&E stained sections disclosed significantly increasing of re-epithelialization, number of fibroblasts, and number of blood vessels in treatment group in comparison to control group (P < 0.05).
  
  
  CONCLUSIONS
  [corrected] The results of this study indicated that allogenous skin fibroblast transplantation can positively affect wound healing in diabetic sheep.
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran. Electronic address: kazemisiamak@yahoo.com.
authors: Siamak
authors: S
authors: Kazemi-Darabadi
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Farshid
authors: F
authors: Sarrafzadeh-Rezaei
authors: Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Amir-Abbas
authors: AA
authors: Farshid
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Bahram
authors: B
authors: Dalir-Naghadeh
mesh_terms: D000496:Alloxan; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000150:complications / Q000503:physiopathology*; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000637:transplantation*; D008297:Male; D011897:Random Allocation; D012756:Sheep; D016038:Skin Transplantation / Q000379:methods*; D014945:Wound Healing / Q000502:physiology*; D014950:Wounds, Penetrating / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*
chemical_list: D000496:Alloxan
keywords: Alloxan; BrdU; Diabetes; Sheep; Skin fibroblasts; Wound healing