title: Comparative population structure analysis of Campylobacter jejuni from human and poultry origin in Bangladesh.
abstract: Campylobacter jejuni is the most important cause of antecedent infections leading to Guillain-Barré syndrome (GBS) and Miller Fisher syndrome (MFS). The objective of the present study was to define the genetic diversity, population structure, and potential role of poultry in the transmission of Campylobacter to humans in Bangladesh. We determined the population structure of C. jejuni isolated from poultry (n = 66) and patients with enteritis (n = 39) or GBS (n = 10). Lipooligosaccharide (LOS) typing showed that 50/66 (76 %) C. jejuni strains isolated from poultry could be assigned to one of five LOS locus classes (A-E). The distribution of neuropathy-associated LOS locus classes A, B, and C were 30/50 (60 %) among the typable strains isolated from poultry. The LOS locus classes A, B, and C were significantly associated with GBS and enteritis-related C. jejuni strains more than for the poultry strains [(31/38 (82 %) vs. 30/50 (60 %), p < 0.05]. Multilocus sequence typing (MLST) defined 15 sequence types (STs) and six clonal complexes (CCs) among poultry isolates, including one ST-3740 not previously documented. The most commonly identified type, ST-5 (13/66), in chicken was seen only once among human isolates (1/49) (p < 0.001). Amplified fragment length polymorphism (AFLP) revealed three major clusters (A, B, and C) among C. jejuni isolated from humans and poultry. There seems to be a lack of overlap between the major human and chicken clones, which suggests that there may be additional sources for campylobacteriosis other than poultry in Bangladesh.
authors: Emerging Diseases and Immunobiology Research Group, Centre for Food and Waterborne Diseases (CFWD), International Centre for Diarrheal Diseases Research (ICDDR,B), GPO Box 128, Dhaka, 1000, Bangladesh, zislam@icddrb.org.
authors: Z
authors: Z
authors: Islam
authors: A
authors: A
authors: van Belkum
authors: J A
authors: JA
authors: Wagenaar
authors: A J
authors: AJ
authors: Cody
authors: A G
authors: AG
authors: de Boer
authors: S K
authors: SK
authors: Sarker
authors: B C
authors: BC
authors: Jacobs
authors: K A
authors: KA
authors: Talukder
authors: H P
authors: HP
authors: Endtz
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000382:microbiology* / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002645:Chickens; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry; D010802:Phylogeny; D011201:Poultry Diseases / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides