title: [Preparation of human meniscus acellular matrix].
abstract: OBJECTIVE
  To investigate development of a cell extraction process for preparing human meniscus acellular matrix, and morphology and biomechanical properties.
  
  
  METHODS
  Human meniscus were subjected to modified eight-step detergent, then, the specimens were assessed by staining with haematoxylin-eosin, toluidine blue, sirius red, saffron O, alcain blue and hoechst-33258, et al. The ultrastructure of the specimens was observed with scanning electron microscope. Transient recovery rate of deformation, maximal recovery rate of deformation and maximal compressive strength were tested to determine the biomechanical properties of the scaffold.
  
  
  RESULTS
  Every stain confirmed that the celluar constituents of the specimens were removed. The specimens stained positively by staining with sirius red. Lacuna were found irregularly not only on the surface of the meniscus,but also in the meniscus with scanning electron microscope. Pores in the specinmens were large, the diameter of pores was 80 to 760 microm, porosity was over 67%. The transient recovery rate of deformation was (89.62 +/- 1.04)%, the maximal recovery rate of deformation was 100% and the maximal compressive strength was (3.04 +/- 0.13)N, when the specimens were compressed 30%.
  
  
  CONCLUSIONS
  The modified eight-step detergent can remove the immunogenic cell components from human meniscus, in addition, 3D extracellular matrix can be retained. The scaffold has good biomechanical properties. This scaffold stands a good chance to be an implant for future tissue engineering of the human meniscus.
authors: Yu
authors: Y
authors: Zhou
authors: Yu-Jie
authors: YJ
authors: Liu
authors: Jing-Xiang
authors: JX
authors: Huang
authors: Rui
authors: R
authors: Wang
authors: Lei
authors: L
authors: Zang
authors: Yong
authors: Y
authors: Hai
mesh_terms: D000328:Adult; D002469:Cell Separation / Q000379:methods*; D002477:Cells / Q000737:chemistry / Q000166:cytology; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D008592:Menisci, Tibial / Q000166:cytology*; D013194:Staining and Labeling