title: Virological characterization of HIV-2 vpx gene mutants in various cell systems.
abstract: Requirement of intrinsically disordered protein Vpx for HIV-2 replication is cell-type dependent. To define Vpx-dependent conditions, replication ability of HIV-2 vpx mutants was analyzed in various cell lines that differ in cellular type, differentiation state and/or expression level of anti-HIV-1 SAMHD1 degraded by Vpx. Induction of Vpx-sensitive anti-HIV-2 state was not always associated with SAMHD1 expression. Compared with our previous data in lymphocytic cells, growth-defectiveness of the vpx mutants in differentiated THP-1 cells, a newly established multi-cycle infection system, was considerably different. Taken together, our results suggest that Vpx plays cell-type dependent role through its undetermined structure and/or function.
authors: Department of Microbiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan.
authors: Masako
authors: M
authors: Nomaguchi
authors: Department of Microbiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan; Japanese Foundation for AIDS Prevention, Chiyoda-ku, Tokyo 101-0061, Japan.
authors: Naoya
authors: N
authors: Doi
authors: Department of Microbiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan. Electronic address: adachi@basic.med.tokushima-u.ac.jp.
authors: Akio
authors: A
authors: Adachi
mesh_terms: D000818:Animals; D002460:Cell Line; D015498:HIV-2 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D008252:Macaca fascicularis; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics* / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D050505:Mutant Proteins; C074286:VPX protein, Human immunodeficiency virus 2; D054334:Viral Regulatory and Accessory Proteins; D020559:Monomeric GTP-Binding Proteins
keywords: HIV-2; Natural target cells; SIVmac; Target protein; Vpx