title: Hematological changes in opium addicted diabetic rats.
abstract: BACKGROUND
  Chronic opioid treatment in animal models has shown to alter hematological parameters.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the biological effects of opium on the number of peripheral blood cells and red blood cells (RBCs) indices in diabetic rats.
  
  
  METHODS
  Peripheral blood samples were collected from diabetic, opium-addicted, diabetic opium-addicted and normal male and female rats and hematological parameters were measured.
  
  
  RESULTS
  The mean number of white blood cells (WBCs) was significantly higher in diabetic opium-addict females compared to diabetic non-addict female group. In both male and female, the mean number of neutrophils was significantly higher and the mean number of lymphocytes was lower in diabetic opium-addicted rats than those observed in diabetic non-addicted group. In diabetic opium-addicted male group the mean counts of RBC significantly increased as compared with diabetic male group. However, in diabetic addicted female, the mean number of RBCs was significantly lower than diabetic non-addicted female group. In both males and females, the mean number of platelets was significantly lower in diabetic addict rats compared to diabetic non-addict group.
  
  
  CONCLUSIONS
  Generally, the results indicated that opium addiction has different effects on male and female rats according to the number of WBC, RBC and RBC indices. It could also be concluded that in the opium-addicts the risk of infection is enhanced due to the weakness of immune system as a result of the imbalance effect of opium on the immune cells.
authors: Department of Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran ; Physiology Research Center, Kerman University of Medical Sciences, Kerman, IR Iran.
authors: Gholamreza
authors: G
authors: Asadikaram
authors: Department of Biochemistry, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran.
authors: Majid
authors: M
authors: Sirati-Sabet
authors: Department of Biochemistry, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran.
authors: Majid
authors: M
authors: Asiabanha
authors: Physiology Research Center, Kerman University of Medical Sciences, Kerman, IR Iran ; Department of Physiology, Kerman University of Medical Sciences, Kerman, IR Iran.
authors: Nader
authors: N
authors: Shahrokhi
authors: Department of Immunology, Rafsanjan University of Medical Sciences, Kerman, IR Iran.
authors: Abdollah
authors: A
authors: Jafarzadeh
authors: Physiology Research Center, Kerman University of Medical Sciences, Kerman, IR Iran ; Department of Physiology, Kerman University of Medical Sciences, Kerman, IR Iran.
authors: Mohammad
authors: M
authors: Khaksari
keywords: Blood Cell Count; Diabetes Mellitus; Drug Addiction; Hematological; Opium