title: Role of far upstream element binding protein 1 in colonic epithelial disruption during dextran sulphate sodium-induced murine colitis.
abstract: OBJECTIVE
  Intestinal epithelial barrier is essential for maintaining normal intestinal homeostasis; its breakdown leads to chronic inflammatory pathologies, such as inflammatory bowel diseases. Far upstream element binding protein 1 (FBP1) has been reported to play an important role in cell apoptosis and proliferation. We aimed to investigate the expression and the role of FBP1 in dextran sodium sulphate (DSS)-induced experimental colitis.
  
  
  METHODS
  Mice experimental colitis model was established by administration of DSS, and the expression and localization of FBP1 was examined using Western blot and immunohistochemistry. Colon epithelial cell line HT-29 was used to determine the role of FBP1. In vitro study, the expression of FBP1 was determined in HT-29 cells stimulated with tumor necrosis factor α (TNF-α). HT-29 cells were transfected with FBP1 siRNA and then measured for viability.
  
  
  RESULTS
  Significant decreasing of FBP1 expression was found in mice colitis. In addition, FBP1 was cleaved and translocated from nucleus to cytoplasm during apoptosis. Downregulated expression of FBP1 induced cell cycle arrest.
  
  
  CONCLUSIONS
  We demonstrate that apoptosis-mediated cleavage of FBP1 and its decreased expression in epithelial cells induces cell cycle arrest, which may play an important role in colonic epithelial disruption.
authors: Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University 300 Guangzhou Road, Nanjing 210000, Jiangsu Province, People's Republic of China.
authors: Qiyun
authors: Q
authors: Tang
authors: Department of Gastroenterology, Affiliated Hospital of Nantong University 20 Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
authors: Weiwei
authors: W
authors: Xia
authors: Department of Gastroenterology, Affiliated Hospital of Nantong University 20 Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
authors: Qianqian
authors: Q
authors: Ji
authors: Department of Gastroenterology, Affiliated Hospital of Nantong University 20 Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
authors: Runzhou
authors: R
authors: Ni
authors: Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University 300 Guangzhou Road, Nanjing 210000, Jiangsu Province, People's Republic of China.
authors: Jian'an
authors: J
authors: Bai
authors: Department of Gastroenterology, Affiliated Hospital of Nantong University 20 Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
authors: Liren
authors: L
authors: Li
authors: Department of Pathogen Biology, Nantong University Medical College 19 Qixiu Road, Nantong 226001, Jiangsu Province, People's Republic of China.
authors: Yongwei
authors: Y
authors: Qin
mesh_terms: D000818:Animals; D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D002470:Cell Survival; D003092:Colitis / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D015536:Down-Regulation; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D005260:Female; D019073:HT29 Cells; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D034622:RNA Interference; D016601:RNA-Binding Proteins; D013997:Time Factors; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D004268:DNA-Binding Proteins; C492258:FUBP1 protein, human; C118921:Fubp protein, mouse; D016601:RNA-Binding Proteins; D014409:Tumor Necrosis Factor-alpha; D016264:Dextran Sulfate; D004265:DNA Helicases
keywords: Colitis; FBP1; intestinal epithelial cells