title: Changes in meat quality traits and sarcoplasmic proteins during aging in three different cattle breeds.
abstract: The effects of breed and aging time (1, 7, 14, 21d) were evaluated on physical meat properties and on sarcoplasmic protein changes in 24 young bulls from Romagnola×Podolian, Podolian and Friesian breeds. Aging affects lightness showing an increase in all breeds while changes in redness varied according to the breed. Podolian breed showed meat with the darkest and the reddest color and the lowest drip loss compared to the other breeds. Extending aging to 21d reduced drip loss from meat. SDS-PAGE and 2DE showed that many changes in the sarcoplasmic proteins occurred among breeds and during aging. During post-mortem some sarcoplasmic proteins decline in intensity after 21d highlighting that they were susceptible to aging. Protein identification and western blotting showed the presence of myosin light chains, Troponin T and tropomyosin proteins during aging, suggesting a degradation of myofibers and a more intense proteolysis especially in the Podolian breed.
authors: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, University of Foggia, Via, Napoli, 25, 71121 Foggia, Italy. Electronic address: rosaria.marino@unifg.it.
authors: R
authors: R
authors: Marino
authors: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, University of Foggia, Via, Napoli, 25, 71121 Foggia, Italy.
authors: M
authors: M
authors: Albenzio
authors: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, University of Foggia, Via, Napoli, 25, 71121 Foggia, Italy.
authors: A
authors: A
authors: della Malva
authors: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, University of Foggia, Via, Napoli, 25, 71121 Foggia, Italy.
authors: M
authors: M
authors: Caroprese
authors: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, University of Foggia, Via, Napoli, 25, 71121 Foggia, Italy.
authors: A
authors: A
authors: Santillo
authors: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, University of Foggia, Via, Napoli, 25, 71121 Foggia, Italy.
authors: A
authors: A
authors: Sevi
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle / Q000145:classification; D003116:Color; D004591:Electrophoresis, Polyacrylamide Gel; D063427:Food Quality; D006863:Hydrogen-Ion Concentration; D008297:Male; D008460:Meat / Q000032:analysis*; D032342:Muscle Cells / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry; D009218:Myosins / Q000737:chemistry; D010641:Phenotype; D059748:Proteolysis; D040901:Proteomics; D020107:Troponin T / Q000737:chemistry; D014867:Water / Q000032:analysis
chemical_list: D020107:Troponin T; D014867:Water; D009218:Myosins
keywords: Aging; Breed; Proteolysis; Sarcoplasmic proteins; Water holding capacity