title: 100 Gy 60 Co γ-ray induced novel mutations in tetraploid wheat.
abstract: 10 accessions of tetraploid wheat were radiated with 100 Gy (60)Co γ -ray. The germination energy, germination rate, special characters (secondary tillering, stalk with wax powder, and dwarf), meiotic process, and high-molecular-weight glutenin subunits (HMW-GSs) were observed. Different species has different radiation sensibility. With 1 seed germinated (5%), T. dicoccum (PI434999) is the most sensitive to this dose of radiation. With a seed germination rate of 35% and 40%, this dose also affected T. polonicum (As304) and T. carthlicum (As293). Two mutant dwarf plants, T. turgidum (As2255) 253-10 and T. polonicum (As302) 224-14, were detected. Abnormal chromosome pairings were observed in pollen mother cells of both T. dicoccoides (As835) 237-9 and T. dicoccoides (As838) 239-8 with HMW-GS 1Ax silent in seeds from them. Compared with the unirradiated seed of T. polonicum (As304) CK, a novel HMW-GS was detected in seed of T. polonicum (As304) 230-7 and its electrophoretic mobility was between 1By8 and 1Dy12 which were the HMW-GSs of Chinese Spring. These mutant materials would be resources for wheat breeding.
authors: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China ; Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
authors: Chuntao
authors: C
authors: Yang
authors: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
authors: Jianshu
authors: 0000-0002-1483-292X
authors: J
authors: Zhu
authors: Institute of Biological and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610061, China.
authors: Yun
authors: Y
authors: Jiang
authors: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
authors: Xiaolu
authors: X
authors: Wang
authors: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
authors: Mengxue
authors: M
authors: Gu
authors: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
authors: Yi
authors: Y
authors: Wang
authors: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
authors: Houyang
authors: H
authors: Kang
authors: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
authors: Xing
authors: X
authors: Fan
authors: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
authors: Lina
authors: L
authors: Sha
authors: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
authors: Haiqin
authors: H
authors: Zhang
authors: Institute of Biological and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610061, China.
authors: Pu
authors: P
authors: Xuan
authors: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China ; Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
authors: Yonghong
authors: Y
authors: Zhou
mesh_terms: D003037:Cobalt Radioisotopes; D005720:Gamma Rays / Q000009:adverse effects*; D018525:Germination / Q000235:genetics / Q000528:radiation effects; D008938:Mitosis; D009154:Mutation / Q000528:radiation effects*; D010641:Phenotype; D011829:Radiation Dosage; D057891:Tetraploidy; D014908:Triticum / Q000235:genetics* / Q000528:radiation effects*
chemical_list: D003037:Cobalt Radioisotopes