title: Tracking the composition and dominant components of the microbial community via polymerase chain reaction-denaturing gradient gel electrophoresis and fluorescence in situ hybridization during vermiconversion for liquid-state excess sludge stabilization.
abstract: To quantitatively explore the microbial community modified by earthworms, a vermifilter (VF, with earthworms) and a conventional biofilter (BF, without earthworms) were continuously operated to stabilize excess sludge. The results demonstrated a positive role imposed by earthworms on compositions and dominant components of microbial community in the VF. For one thing, the phyla Actinobacteria and Acidobacteria were only detected in the VF, which might explain for the higher Shannon index of bacteria in the VF (H = 2.58) than that in the BF (H = 1.99). For another, the total proportion of dominant bacteria in the VF increased by 23% compared to the BF. Moreover, quantification analysis explicitly noted that the dominant bacteria in VF were β-proteobacteria (27 ± 2%) and γ-proteobacteria (24 ± 1%) while that in BF was Bacteroidetes (21 ± 1%). In conclusion, stimulated by earthworms, a unique microbial community developed in the VF, thus improving the stabilization of excess sludge.
authors: State Key Laboratory of Pollution Control and Resources Reuse, 1239 Siping Road, Shanghai, China; The Institute of Biofilm Technology, Key Laboratory of Yangtze River Water Environment, Ministry of Education, 1239 Siping Road, Shanghai,China; College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors: Ting
authors: T
authors: Xu
authors: State Key Laboratory of Pollution Control and Resources Reuse, 1239 Siping Road, Shanghai, China; The Institute of Biofilm Technology, Key Laboratory of Yangtze River Water Environment, Ministry of Education, 1239 Siping Road, Shanghai,China; College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. Electronic address: xingmeiyan@tongji.edu.cn.
authors: Meiyan
authors: M
authors: Xing
authors: State Key Laboratory of Pollution Control and Resources Reuse, 1239 Siping Road, Shanghai, China; The Institute of Biofilm Technology, Key Laboratory of Yangtze River Water Environment, Ministry of Education, 1239 Siping Road, Shanghai,China; College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors: Jian
authors: J
authors: Yang
authors: State Key Laboratory of Pollution Control and Resources Reuse, 1239 Siping Road, Shanghai, China; The Institute of Biofilm Technology, Key Laboratory of Yangtze River Water Environment, Ministry of Education, 1239 Siping Road, Shanghai,China; College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors: Baoyi
authors: B
authors: Lv
authors: State Key Laboratory of Pollution Control and Resources Reuse, 1239 Siping Road, Shanghai, China; The Institute of Biofilm Technology, Key Laboratory of Yangtze River Water Environment, Ministry of Education, 1239 Siping Road, Shanghai,China; College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors: Ting
authors: T
authors: Duan
authors: Shanghai Municipal Sewerage Company Ltd., 1121 Yishan Road, Shanghai, China.
authors: Jing
authors: J
authors: Nie
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics*; D018441:Biofilms; D016000:Cluster Analysis; D058645:Denaturing Gradient Gel Electrophoresis; D005798:Genes, Bacterial; D017404:In Situ Hybridization, Fluorescence; D009835:Oligochaeta / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S; D012722:Sewage
keywords: Fluorescence in situ hybridization (FISH); Microbial community structure; Polymerase chain reaction–denaturing gradient gel electrophoresis (PCR–DGGE); Vermifilter