title: Genetic alterations of chromosomes, p53 and p16 genes in low- and high-grade bladder cancer.
abstract: A majority of patients with bladder cancer present with superficial disease and subsequently, some patients show progression to muscle invasive or metastatic disease. Bladder cancer has a complex genetic process and identification of the genetic alterations which occur during progression may lead to the understanding of the nature of the disease and provide the possibility of early treatment. The aim of the present study was to compare the structural and numerical chromosomal differences and changes in the p16 and p53 genes between low-grade (LG) and high-grade (HG) bladder cancer (BC) using cytogenetic and molecular cytogenetic methods. Between March 2009 and March 2010, cytogenetic analyses were carried out on tumor and blood samples in 34 patients with transitional cell type BC, and on blood samples of 34 healthy patients as a control group. Fluorescencein situhybridization probes for the p16 and p53 genes were also used to screen the alterations in these genes in 32 patients with BC. The patients were divided into two groups (LG and HG) and the findings were compared. A total of 11 (32.3%) patients exhibited LGBC, 22 (64.7%) exhibited HGBC and one (3%) patient exhibited carcinomain situ. There were no differences between the LGBC and HGBC groups according to the number of chromosomal aberrations (P=0.714); however, differences between alterations of the p16 and p53 genes were significant (P=0.002 and P=0.039). Almost all structural abnormalities were found to be located to the 1q21, 1q32, 3p21 and 5q31 regions in patients with HG tumors. In conclusion, the p16 and p53 genes were altered more prominently in patients with HG tumors compared with LG tumors. The structural abnormalities in the 1q21, 1q32, 3p21 and 5q31 regions were observed more frequently in patients with HG tumors. These regions may play significant roles in the progression of BC, but further studies are required to find candidate genes for a panel of BC.
authors: Department of Urology, Faculty of Medicine, Çukurova University, 01330 Adana, Turkey.
authors: Deniz
authors: D
authors: Abat
authors: Department of Medical Biology, Faculty of Medicine, Çukurova University, 01330 Adana, Turkey.
authors: Osman
authors: O
authors: Demirhan
authors: Department of Medical Biology, Faculty of Medicine, Çukurova University, 01330 Adana, Turkey.
authors: Nihal
authors: N
authors: Inandiklioglu
authors: Department of Medical Biology, Faculty of Medicine, Çukurova University, 01330 Adana, Turkey.
authors: Erdal
authors: E
authors: Tunc
authors: Department of Pathology, Faculty of Medicine, Çukurova University, 01330 Adana, Turkey.
authors: Seyda
authors: S
authors: Erdogan
authors: Vocational School of Health Services, Adıyaman University, 02040 Adıyaman, Turkey.
authors: Deniz
authors: D
authors: Tastemir
authors: Department of Medical Biology, Faculty of Medicine, Çukurova University, 01330 Adana, Turkey.
authors: Inayet Nur
authors: IN
authors: Uslu
authors: Department of Urology, Faculty of Medicine, Çukurova University, 01330 Adana, Turkey.
authors: Zuhtu
authors: Z
authors: Tansug
keywords: bladder carcinoma; chromosomal aberrations; fluorescence in situ hybridization; high grade; low grade; p16; p53