title: The PLAU P141L single nucleotide polymorphism is associated with collateral circulation in patients with coronary artery disease.
abstract: OBJECTIVE
  Urokinase-type plasminogen activator, which is encoded by the PLAU gene, plays a prominent role during collateral arterial growth. We investigated whether the PLAU P141L (C > T) polymorphism, which causes a mutation in the kringle domain of the protein, is associated with coronary collateral circulation in a cohort of 676 patients with coronary artery disease.
  
  
  METHODS
  The polymorphism was genotyped in blood samples using a TaqMan-based genotyping assay, and collateral circulation was assessed by the Rentrop method. Multivariate logistic regression models adjusted by clinically relevant variables to estimate odds ratios were used to examine associations of PLAU P141L allelic variants and genotypes with collateral circulation.
  
  
  RESULTS
  Patients with poor collateral circulation (Rentrop 0-1; n = 547) showed a higher frequency of the TT genotype than those with good collateral circulation (Rentrop 2-3; n = 129; P = .020). The T allele variant was also more common in patients with poor collateral circulation (P = .006). The odds ratio of having poorly developed collaterals in patients bearing the T allele (adjusted for clinically relevant variables) was statistically significant under the dominant model (odds ratio = 1.83 [95% confidence interval, 1.16-2.90]; P = .010) and the additive model (odds ratio = 1.73 [95% confidence interval, 1.14-2.62]; P = .009).
  
  
  CONCLUSIONS
  An association was found between coronary collateral circulation and the PLAU P141L polymorphism. Patients with the 141L variant are at greater risk of developing poor coronary collateral circulation.
authors: Unidad de Anatomía y Embriología Humanas, Departamento de Patología y Terapéutica Experimental, Facultad de Medicina, Campus de Ciencias de la Salud de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, Spain.
authors: Joan
authors: J
authors: Duran
authors: Unidad de Anatomía y Embriología Humanas, Departamento de Patología y Terapéutica Experimental, Facultad de Medicina, Campus de Ciencias de la Salud de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, Spain; Departamento de Estadística, Universidad de Valparaíso, Valparaíso, Chile.
authors: Pilar
authors: P
authors: Sánchez-Olavarría
authors: Unidad de Anatomía y Embriología Humanas, Departamento de Patología y Terapéutica Experimental, Facultad de Medicina, Campus de Ciencias de la Salud de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, Spain; Grupo de Investigación Neurovascular (NEUVAS), IMIM-Hospital del Mar, PRBB-Parque de Investigación Biomédica de Barcelona, Barcelona, Spain.
authors: Marina
authors: M
authors: Mola
authors: Unidad de Anatomía y Embriología Humanas, Departamento de Patología y Terapéutica Experimental, Facultad de Medicina, Campus de Ciencias de la Salud de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, Spain.
authors: Víctor
authors: V
authors: Götzens
authors: Departamento de Cardiología y Hemodinamia, Centro Cardiovascular Sant Jordi, Barcelona, Spain.
authors: Julio
authors: J
authors: Carballo
authors: Departamento de Cardiología y Hemodinamia, Centro Cardiovascular Sant Jordi, Barcelona, Spain.
authors: Eva
authors: E
authors: Martín-Pelegrina
authors: Departamento de Cardiología y Hemodinamia, Centro Cardiovascular Sant Jordi, Barcelona, Spain.
authors: Màrius
authors: M
authors: Petit
authors: Departamento de Cardiología, Hospital Universitari Vall d'Hebron, Barcelona, Spain.
authors: Bruno
authors: B
authors: García Del Blanco
authors: Departamento de Cardiología, Hospital Universitari Vall d'Hebron, Barcelona, Spain.
authors: David
authors: D
authors: García-Dorado
authors: Unidad de Anatomía y Embriología Humanas, Departamento de Patología y Terapéutica Experimental, Facultad de Medicina, Campus de Ciencias de la Salud de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, Spain. Electronic address: janta@ub.edu.
authors: Josep M
authors: JM
authors: de Anta
mesh_terms: D000368:Aged; D003097:Collateral Circulation / Q000235:genetics* / Q000528:radiation effects; D017023:Coronary Angiography; D003327:Coronary Disease / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics* / Q000502:physiology; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics* / Q000502:physiology
chemical_list: D014568:Urokinase-Type Plasminogen Activator
keywords: Activador del plasminógeno; Association study; Circulación colateral; Collateral circulation; Estudio de asociación; Plasminogen activator; Polimorfismo rs2227564; rs2227564 polymorphism