title: Clinicopathological and prognostic implications of the miR-200 family in patients with epithelial ovarian cancer.
abstract: The aim of the present study was to investigate the association of the expression of members in the miR-200 family with clinicopathological characteristics and their impacts on overall survival in patients with epithelial ovarian cancer (EOC). Expression levels of members in the miR-200 family, including miR-200a, miR-200b, miR-200c, miR-141, and miR-429, were detected by using miRNA qRT-PCR and in situ hybridization. Associations of their expression with clinicopathological factors and overall survival were statistically evaluated. Among five members in the miR-200 family, the expression levels of miR-200a, miR-200b and miR-200c were significantly higher in EOC tissues than those in normal surface ovarian epithelium tissues, in line with the findings ofin situ hybridization analysis. In addition, tumors with high miR-200a and miR-200 bexpressionwere both more likely to have advanced stage (both P=0.006) and higher grade (P=0.01 and 0.02), whilehighmiR-200 cexpression was onlysignificantly associated with advanced stage disease (P=0.01). Moreover, univariate analysis showed that the patients with high miR-200a, miR-200b and miR-200c expression all correlated with shorter overall survival in EOC patients (all P<0.001). Multivariate statistical analysis further identified miR-200a, miR-200b and miR-200c asindependent prognostic factorsfor EOC (all P=0.01). In conclusion, these findings suggest that miR-200a, miR-200b and miR-200c overexpression may promote the aggressive tumor progression and be recognized as reliable markers to predict the survival in patients with EOCs. The three miRNAs could be attractive therapeutic targets in patients with advanced-stage EOCs.
authors: Department of Obstetrics and Gynecology, The 180th Hospital of Chinese People's Liberation Army Hua-Yuan Road No.180, Quanzhou City, Fujian Province, 362000 China.
authors: Qing
authors: Q
authors: Cao
authors: Department of Obstetrics and Gynecology, The 180th Hospital of Chinese People's Liberation Army Hua-Yuan Road No.180, Quanzhou City, Fujian Province, 362000 China.
authors: Kunlin
authors: K
authors: Lu
authors: Department of Obstetrics and Gynecology, The 180th Hospital of Chinese People's Liberation Army Hua-Yuan Road No.180, Quanzhou City, Fujian Province, 362000 China.
authors: Suiping
authors: S
authors: Dai
authors: Department of Obstetrics and Gynecology, The 180th Hospital of Chinese People's Liberation Army Hua-Yuan Road No.180, Quanzhou City, Fujian Province, 362000 China.
authors: Yan
authors: Y
authors: Hu
authors: Department of Obstetrics and Gynecology, The 180th Hospital of Chinese People's Liberation Army Hua-Yuan Road No.180, Quanzhou City, Fujian Province, 362000 China.
authors: Weifang
authors: W
authors: Fan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D000077216:Carcinoma, Ovarian Epithelial; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D053208:Kaplan-Meier Estimate; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C533896:MIRN200 microRNA, human; D035683:MicroRNAs
keywords: clinicopathology; epithelial ovarian cancer; miR-200 family; prognosis