title: Exercise training improves high-density lipoprotein-mediated transcription of proangiogenic microRNA in endothelial cells.
abstract: BACKGROUND
  The functional properties of endothelial cells (ECs) for regulating nitric oxide (NO) bioavailability are important for normal endothelial function. Micro-RNAs (miRs) and especially angiomiRs regulate vascular integrity and angiogenesis. Besides regulation of reverse cholesterol transport, high-density lipoprotein (HDL) also stimulates NO generation by ECs. This function is impaired in patients with chronic heart failure (CHF) and can be attenuated by exercise training. The aim of the present study was to evaluate if HDL-induced miR expression is altered in CHF and if exercise training has an impact.
  
  
  METHODS
  HDL was isolated from CHF patients in NYHA-IIIb (HDLNYHA) and healthy subjects (HDLHealthy) before and after exercise training. Subsequently ECs were incubated for 24 h with the isolated HDL and miR expression was quantified by RT-PCR.
  
  
  RESULTS
  HDL-induced expression of miR-126, miR-21 and miR-222 was significantly reduced in ECs incubated with HDLNYHA when compared to HDLHealthy. Exercise training attenuated this HDL-induced reduction of miR-126 and miR-21. HDL-induced expression of miR-221 and miR-214 was not altered in CHF compared to controls and no impact of exercise training was noted.
  
  
  CONCLUSIONS
  In conclusion, the present study shows that HDL isolated from CHF patients (NYHA-III) reduces the expression of pro-angiogenic miRs (i.e. miR-126 and miR-21), which may contribute to atherogenesis and endothelial dysfunction. However, exercise training was able to attenuate the HDL-induced reduction in pro-angiogenic miRs expression.
authors: Department of Cardiology, University Leipzig - Heart Center Leipzig, Leipzig, Germany.
authors: Saskia
authors: S
authors: Riedel
authors: Department of Cardiology, University Leipzig - Heart Center Leipzig, Leipzig, Germany.
authors: Stephan
authors: S
authors: Radzanowski
authors: Department of Cardiology, University Leipzig - Heart Center Leipzig, Leipzig, Germany.
authors: T Scott
authors: TS
authors: Bowen
authors: Department of Cardiology, University Leipzig - Heart Center Leipzig, Leipzig, Germany.
authors: Sarah
authors: S
authors: Werner
authors: Department of Cardiology, University Leipzig - Heart Center Leipzig, Leipzig, Germany.
authors: Sandra
authors: S
authors: Erbs
authors: Department of Cardiology, University Leipzig - Heart Center Leipzig, Leipzig, Germany.
authors: Gerhard
authors: G
authors: Schuler
authors: Department of Cardiology, University Leipzig - Heart Center Leipzig, Leipzig, Germany adav@medizin.uni-leipzig.de.
authors: Volker
authors: V
authors: Adams
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D005081:Exercise Therapy; D005260:Female; D006333:Heart Failure / Q000097:blood / Q000175:diagnosis / Q000235:genetics / Q000503:physiopathology / Q000628:therapy*; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014158:Transcription, Genetic; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D008075:Lipoproteins, HDL; C527111:MIRN126 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs
keywords: High density lipoprotein; chronic heart failure; exercise training; microRNA