title: Interaction between NOD2 and CARD9 involves the NOD2 NACHT and the linker region between the NOD2 CARDs and NACHT domain.
abstract: NOD2 activation by muramyl dipeptide causes a proinflammatory immune response in which the adaptor protein CARD9 works synergistically with NOD2 to drive p38 and c-Jun N-terminal kinase (JNK) signalling. To date the nature of the interaction between NOD2 and CARD9 remains undetermined. Here we show that this interaction is not mediated by the CARDs of NOD2 and CARD9 as previously suggested, but that NOD2 possesses two interaction sites for CARD9; one in the CARD-NACHT linker and one in the NACHT itself.
authors: Department of Biochemistry, University of Cambridge, Cambridge, UK.
authors: Rhiannon
authors: R
authors: Parkhouse
authors: Department of Biochemistry, University of Cambridge, Cambridge, UK.
authors: Joseph P
authors: JP
authors: Boyle
authors: Department of Biochemistry, University of Cambridge, Cambridge, UK.
authors: Sophie
authors: S
authors: Mayle
authors: Division of Molecular Structure, MRC-National Institute for Medical Research, London, UK.
authors: Kovilen
authors: K
authors: Sawmynaden
authors: Division of Molecular Structure, MRC-National Institute for Medical Research, London, UK.
authors: Katrin
authors: K
authors: Rittinger
authors: Department of Biochemistry, University of Cambridge, Cambridge, UK; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK. Electronic address: tpm22@cam.ac.uk.
authors: Tom P
authors: TP
authors: Monie
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D053468:CARD Signaling Adaptor Proteins; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D053473:Nod2 Signaling Adaptor Protein / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D053468:CARD Signaling Adaptor Proteins; C513280:Card9 protein, mouse; D053473:Nod2 Signaling Adaptor Protein
keywords: Caspase activation and recruitment domain; Crohn’s Disease; Innate immunity; Nucleotide-binding leucine-rich repeat containing receptor; Signal transduction; Stress kinase pathway