title: Differences in IGF axis-related proteins in amniotic fluid of trisomy 21 and trisomy 18 using a multiple reaction monitoring approach.
abstract: OBJECTIVE
  Trisomy 21 and trisomy 18 are the two most common chromosomal anomalies in live births. To find new biomarkers for aneuploidies and pathogenesis of fetal malformations, we measured insulin-like growth factor (IGF) axis-related proteins in amniotic fluid (AF) of pregnant women carrying trisomies 21 or 18 affected fetuses using multiple reaction monitoring (MRM) approach.
  
  
  METHODS
  Eighty-five AF samples from pregnant women carrying either trisomy 21, trisomy 18, or normal fetuses were collected. IGF axis-related proteins in AF after serial treatments were quantitated with MRM method. The differential protein levels were also confirmed by western blot in AF without any treatment.
  
  
  RESULTS
  The IGF type I receptor and pregnancy-associated plasma protein-A in AF of trisomy 21 (1.35 ± 0.32 and 13.36 ± 3.64 µg/mg protein) and trisomy 18 (1.39 ± 0.40 and 12.80 ± 1.84 µg/mg protein) were decreased versus normal controls (2.16 ± 0.59 and 23.77 ± 6.18 µg/mg protein). IGF binding protein 5 was reduced in trisomy 18 (1.47 ± 0.33 vs 2.36 ± 0.77 µg/mg protein). These alterations were confirmed by western blot. The other proteins showed no significant difference between the three groups.
  
  
  CONCLUSIONS
  Our data suggested that MRM can provide a powerful platform for the identification of biomarkers in AF that have crucial developmental effects in the aneuploid fetus.
authors: Department of Clinical Genetics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.
authors: Guangrui
authors: G
authors: Lai
authors: Yuanyuan
authors: Y
authors: Zhang
authors: Leitong
authors: L
authors: Wang
authors: Bijun
authors: B
authors: Zhang
authors: Gaoyan
authors: G
authors: Zheng
authors: Xuanyi
authors: X
authors: Wang
authors: Xiaoqiu
authors: X
authors: Zhao
authors: Haiming
authors: H
authors: Gao
authors: Yanyan
authors: Y
authors: Zhao
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D002887:Chromosomes, Human, Pair 18; D004314:Down Syndrome / Q000175:diagnosis*; D005260:Female; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins / Q000032:analysis*; D011247:Pregnancy; D017451:Receptors, Somatomedin / Q000032:analysis*; D012189:Retrospective Studies; D013002:Somatomedins / Q000032:analysis*; D014314:Trisomy / Q000175:diagnosis*; D000073842:Trisomy 18 Syndrome
chemical_list: D015415:Biomarkers; D018969:Insulin-Like Growth Factor Binding Proteins; D017451:Receptors, Somatomedin; D013002:Somatomedins