title: [Serodiagnosis of human bocavirus lower respiratory tract infection in children].
abstract: OBJECTIVE
  To study the application of serodiagnosis of human bocavirus (HBoV) lower respiratory tract infection in children.
  
  
  METHODS
  From January to April, 2013, samples including serum, sputum and bronchoalveolar lavage fluids (BALFs) were obtained from 714 children hospitalized with ALRI. Serums were tested for HBoV-specific IgG and IgM antibodies by ELISA and all kinds of samples were tested for HBoV DNA by quantitative real-time fluorescent PCR. The results of HBoV serologic tests, viral DNA in sputum and their combination were compared with those of HBoV DNA in serums and/or BALFs, which was considered as the "standard". Their consistence and differences were evaluated, and the diagnostic parameters including sensitivity, specificity, positive predictive value, negative predictive value, consistency rate, Kappa value and J value were calculated. Age distributions of the HBoV positive patients tested by the latter two methods were also compared.
  
  
  RESULTS
  The positive rate of HBoV serology was 13.2% (94/714) . The results of HBoV serology, its DNA in sputum and their combination were all consistent with those of HBoV DNA in serums and/or BALFs (χ(2) = 91.834, 124.662, 138.643, P < 0.001 for all comparisons) . Differences were significant by McNemar test (χ(2) = 23.547, 33.440, 12.410, P all <0.001) . All the diagnostic parameters for single HBoV serologic test or single viral DNA test in sputa were approximate. However, they were improved to 70.4%, 94.8%, 38.0%, 98.6%, 93.7%, 0.463(P < 0.001), 0.65 for sensitivity, specificity, positive predictive value, negative predictive value, consistency rate, Kappa value and J value, respectively, when the methods were combined. HBoV was found positive mainly in children under 3 years of age, especially in the 1 year group. The positive rates were the highest in both group -1 year, and group -3 years was the next. However, the rate was the lowest in group >3 years and in the group -6 months.
  
  
  CONCLUSIONS
  Diagnostic power can be improved and age distribution can be demonstrated when serologic tests were combined with traditional sputum DNA detection in children with HBoV lower respiratory tract infection.
authors: Department of Respiratory Diseases, the Children's Hospital Affiliated to Suchow University, Suzhou 215003, China.
authors: Ling
authors: L
authors: Li
authors: Department of Respiratory Diseases, the Children's Hospital Affiliated to Suchow University, Suzhou 215003, China.
authors: Meijuan
authors: M
authors: Wang
authors: Department of Respiratory Diseases, the Children's Hospital Affiliated to Suchow University, Suzhou 215003, China.
authors: Yongdong
authors: Y
authors: Yan
authors: Department of Respiratory Diseases, the Children's Hospital Affiliated to Suchow University, Suzhou 215003, China.
authors: Xuejun
authors: X
authors: Shao
authors: Department of Respiratory Diseases, the Children's Hospital Affiliated to Suchow University, Suzhou 215003, China.
authors: Fengguo
authors: F
authors: Wan
authors: Department of Respiratory Diseases, the Children's Hospital Affiliated to Suchow University, Suzhou 215003, China.
authors: Jun
authors: J
authors: Xu
authors: Department of Respiratory Diseases, the Children's Hospital Affiliated to Suchow University, Suzhou 215003, China.
authors: Huijiang
authors: H
authors: Shao
authors: Department of Respiratory Diseases, the Children's Hospital Affiliated to Suchow University, Suzhou 215003, China. Email:szdxjiwei@163.com.
authors: Wei
authors: W
authors: Ji
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D017677:Age Distribution; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood*; D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D056661:Human bocavirus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007223:Infant; D008297:Male; D010322:Parvoviridae Infections / Q000175:diagnosis* / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M