title: [Identification of common medicinal snakes in medicated liquor of Guangdong by COI barcode sequence].
abstract: OBJECTIVE
  To identify the common snakes in medicated liquor of Guangdong using COI barcode sequence,and to test the feasibility.
  
  
  METHODS
  The COI barcode sequences of collected medicinal snakes were amplified and sequenced. The sequences combined with the data from GenBank were analyzed for divergence and building a neighbor-joining(NJ) tree with MEGA 5.0.
  
  
  RESULTS
  The genetic distance and NJ tree demonstrated that there were 241 variable sites in these species, and the average (A + T) content of 56.2% was higher than the average (G + C) content of 43.7%. The maximum interspecific genetic distance was 0.2568, and the minimum was 0. 1519. In the NJ tree,each species formed a monophyletic clade with bootstrap supports of 100%.
  
  
  CONCLUSIONS
  DNA barcoding identification method based on the COI sequence is accurate and can be applied to identify the common medicinal snakes.
authors: Jing
authors: J
authors: Liao
authors: Zhi
authors: Z
authors: Chao
authors: Liang
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D058893:DNA Barcoding, Taxonomic; D017931:DNA Primers; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV / Q000235:genetics*; D008419:Materia Medica; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012911:Snakes / Q000145:classification / Q000235:genetics*; D013045:Species Specificity; D014920:Wine
chemical_list: D017931:DNA Primers; D021903:DNA, Ribosomal Spacer; D008419:Materia Medica; D003576:Electron Transport Complex IV