title: Crystal structure of the yeast TRAPP-associated protein Tca17.
abstract: The transport protein particle (TRAPP) is a hetero-multimeric complex involved in the trafficking of COP II (coat protein complex II) vesicles. TRAPP is present in different eukaryotes from yeast to vertebrates and occurs in three distinct modifications with function in different intracellular transport steps. All forms contain a core of five essential subunits, and the different species of TRAPP are formed by the addition of various subunits. A recently identified TRAPP-associated protein, Tca17, is supposed to be involved in the regulation of the transport complex. We have determined the three-dimensional structure of yeast Tca17 by X-ray crystallography at a resolution of 1.8 Å. It adopts the longin fold characteristic for the Bet5 family of TRAPP subunits, and it also shares a binding motif of these for the interaction with other members of the complex. Two alternative models of the localization of Tca17 within TRAPP as well as its potential role in the regulation of TRAPP function by transient integration into the complex are discussed.
  
  
  METHODS
  Structural data are available in the Protein Databank under accession number 3PR6.
authors: Macromolecular Structure and Interaction Group, Max-Delbrück-Center for Molecular Medicine, and Chemistry and Biochemistry Institute, Freie Universität, Berlin, Germany.
authors: Chengcheng
authors: C
authors: Wang
authors: Ulrich
authors: U
authors: Gohlke
authors: Yvette
authors: Y
authors: Roske
authors: Udo
authors: U
authors: Heinemann
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D033921:Vesicular Transport Proteins / Q000737:chemistry*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C000593022:Tca17 protein, S cerevisiae; D033921:Vesicular Transport Proteins
keywords: Sedlin; TRAPP; Tca17; Trs20; vesicle transport