title: Protective effects of glucosamine hydrochloride against free radical-induced erythrocytes damage.
abstract: Glucosamine (GlcN) is an important precursor in the biochemical synthesis of glycosylated proteins and lipids in human body. It gains importance because of its contribution to human health and its multiple biological and therapeutic effects. In this study, the in vitro oxidative hemolysis of rat erythrocyte was used as a model to study the potential protective effect of glucosamine hydrochloride against free radical-induced damage of biological membranes. Glucosamine hydrochloride exhibited dose-dependent DPPH antioxidant activity. Oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by a water-soluble free radical initiator 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) were significantly suppressed by GlcN in a time and dose dependent manner. GlcN also prevented the depletion of cytosolic antioxidant glutathione (GSH) in erythrocytes. These results indicated that glucosamine hydrochloride efficiently protected erythrocytes against free radicals and it could be recommended as a pharmaceutical supplement to alleviate oxidative stress.
authors: Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Khadijeh
authors: K
authors: Jamialahmadi
authors: School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Omid
authors: O
authors: Arasteh
authors: Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Maryam
authors: M
authors: Matbou Riahi
authors: Pharmaceutical Research Center, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Soghra
authors: S
authors: Mehri
authors: Medical Toxicology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Bamdad
authors: B
authors: Riahi-Zanjani
authors: Medical Toxicology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: Karimig@mums.ac.ir.
authors: Gholamreza
authors: G
authors: Karimi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005609:Free Radicals; D005944:Glucosamine / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D050050:Protein Carbonylation; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D005978:Glutathione; D005944:Glucosamine
keywords: AAPH; Erythrocyte; Glucosamine; Hemolysis; Oxidative stress