title: Effect of dietary supplementation of probiotics and palm fruits extracts on the antioxidant enzyme gene expression in the mucosae of gilthead seabream (Sparus aurata L.).
abstract: Antioxidant activity is particularly important, since oxidation is an unavoidable reaction in all living bodies. At present, natural antioxidants to be used on food as an alternative to synthetic ones are being sought. Gilthead seabream (Sparus aurata L.) specimens were fed for 4 weeks with diets enriched with bacterial probiotics (Shewanella putrefaciens Pdp11 and Bacillus sp), single or in combination with Tunisian dates palm fruit extracts. The expression of the main antioxidant enzyme genes (superoxide dismutase, catalase and glutathione reductase) in the mucosae (gut, skin and gill) was evaluated after 2 and 4 weeks. Previously, free radical scavenging and several antioxidant assays were developed to know the antioxidant properties present on the palm fruits extracts. The results demonstrated that experimental diets alter the expression of the studied antioxidant genes, primarily in the gill and skin. Furthermore, the tested probiotics and mainly, the aqueous date palm fruits extracts had significant antioxidant properties based on their protective effect against the levels of intracellular reactive oxygen species, especially when administering during 4 weeks. For this reason, probiotics and date palm fruit extracts may serve as good natural antioxidants and could potentially be considered as a functional food ingredient for fish in farms.
authors: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain. Electronic address: aesteban@um.es.
authors: M A
authors: MA
authors: Esteban
authors: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
authors: H
authors: H
authors: Cordero
authors: Department of Food Science, Veterinary Faculty, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain; CIBERobn (Fisiopatología de la Obesidad y la Nutrición CB12/03/30038) Instituto de Salud Carlos III, Spain.
authors: M
authors: M
authors: Martínez-Tomé
authors: Department of Food Science, Veterinary Faculty, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain; CIBERobn (Fisiopatología de la Obesidad y la Nutrición CB12/03/30038) Instituto de Salud Carlos III, Spain.
authors: A M
authors: AM
authors: Jiménez-Monreal
authors: Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products (LATVPEP), Faculty of Pharmacy, University of Monastir, 5000, Tunisia.
authors: A
authors: A
authors: Bakhrouf
authors: Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products (LATVPEP), Faculty of Pharmacy, University of Monastir, 5000, Tunisia.
authors: A
authors: A
authors: Mahdhi
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D019587:Dietary Supplements; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005980:Glutathione Reductase / Q000378:metabolism; D009092:Mucous Membrane / Q000187:drug effects / Q000378:metabolism*; D065749:Phoeniceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D019936:Probiotics / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D021541:Sea Bream / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017931:DNA Primers; D010936:Plant Extracts; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase
keywords: Antioxidant genes; Date palm fruit extracts; Gilthead seabream (Sparus aurata L.); Probiotics; Teleosts