title: EGF-induced expression of Fused Toes Homolog (FTS) facilitates epithelial-mesenchymal transition and promotes cell migration in ME180 cervical cancer cells.
abstract: The role of Fused Toes Homolog (FTS) in epidermal growth factor (EGF) induced epithelial-mesenchymal transition (EMT) in cervical cancer cells was studied. EGF treatment induced the change of EMT markers and increased cell migration. EGF treatment also increased phosphorylated EGFR and ERK and nuclear level of ATF-2. The binding of ATF-2 to the promoter region of FTS was evidenced after EGF treatment. Pretreatment with PD98059 and gefitinib prevented EGF-induced FTS expression. FTS silencing reduced EMT and cell migration by EGF treatment. These results demonstrate a novel function for FTS in EGF-mediated EMT process.
authors: Department of Radiation Oncology, Chungbuk National University, College of Medicine, Cheongju 361-763, Republic of Korea.
authors: Sridhar
authors: S
authors: Muthusami
authors: Department of Radiation Oncology, Chungbuk National University, College of Medicine, Cheongju 361-763, Republic of Korea.
authors: D S
authors: DS
authors: Prabakaran
authors: Department of Environmental and Tropical Medicine, Konkuk University, College of Medicine, Chungju 380-701, Republic of Korea.
authors: Jae-Ran
authors: JR
authors: Yu
authors: Department of Radiation Oncology, Chungbuk National University, College of Medicine, Cheongju 361-763, Republic of Korea. Electronic address: wynpark@chungbuk.ac.kr.
authors: Woo-Yoon
authors: WY
authors: Park
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects / Q000502:physiology; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*
chemical_list: C486377:AKTIP protein, human; D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D004815:Epidermal Growth Factor; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: Cancer cell migration; EGF; EMT; FTS; MMP