title: Hyperprolinemia induces DNA, protein and lipid damage in blood of rats: antioxidant protection.
abstract: The present study investigated the effects of hyperprolinemia on oxidative damage to biomolecules (protein, lipids and DNA) and the antioxidant status in blood of rats. The influence of the antioxidants on the effects elicited by proline was also examined. Wistar rats received two daily injections of proline and/or vitamin E plus C (6th-28th day of life) and were killed 12h after the last injection. Results showed that hyperprolinemia induced a significant oxidative damage to proteins, lipids and DNA demonstrated by increased carbonyl content, malondialdehyde levels and a greater damage index in comet assay, respectively. The concomitant antioxidants administration to proline treatment completely prevented oxidative damage to proteins, but partially prevented lipids and DNA damage. We also observed that the non-enzymatic antioxidant potential was decreased by proline treatment and partially prevented by antioxidant supplementation. The plasma levels of vitamins E and C significantly increased in rats treated exogenously with these vitamins but, interestingly, when proline was administered concomitantly with vitamin E plus C, the levels of these vitamins were similar to those found in plasma of control and proline rats. Our findings suggest that hyperprolinemia promotes oxidative damage to the three major classes of macromolecules in blood of rats. These effects were accomplished by decrease in non-enzymatic antioxidant potential and decrease in vitamins administered exogenously, which significantly decreased oxidative damage to biomolecules studied. These data suggest that antioxidants may be an effective adjuvant therapeutic to limit oxidative damage caused by proline.
authors: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, ICBS, UFRGS, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil. Electronic address: deiakf@gmail.com.
authors: Andréa G K
authors: AG
authors: Ferreira
authors: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, ICBS, UFRGS, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil.
authors: Emilene B
authors: EB
authors: Scherer
authors: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, ICBS, UFRGS, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil.
authors: Aline A
authors: AA
authors: da Cunha
authors: Universidade Federal do Pampa, BR 472, Km 585, Caixa Postal 118, 97500-970 Uruguaiana, RS, Brazil.
authors: Vanusa
authors: V
authors: Manfredini
authors: Serviço de Genética Médica, HCPA, Ramiro Barcelos 2350, Porto Alegre, RS, 90035-903, Brazil.
authors: Giovana Brondani
authors: GB
authors: Biancini
authors: Serviço de Genética Médica, HCPA, Ramiro Barcelos 2350, Porto Alegre, RS, 90035-903, Brazil.
authors: Camila Simioni
authors: CS
authors: Vanzin
authors: Serviço de Genética Médica, HCPA, Ramiro Barcelos 2350, Porto Alegre, RS, 90035-903, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas, UFRGS, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil.
authors: Carmen R
authors: CR
authors: Vargas
authors: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, ICBS, UFRGS, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil. Electronic address: wyse@ufrgs.br.
authors: Angela T S
authors: AT
authors: Wyse
mesh_terms: D050842:1-Pyrroline-5-Carboxylate Dehydrogenase / Q000172:deficiency; D000592:Amino Acid Metabolism, Inborn Errors / Q000503:physiopathology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects*; D019587:Dietary Supplements; D008055:Lipids / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D011392:Proline / Q000737:chemistry; D011394:Proline Oxidase / Q000172:deficiency*; D011506:Proteins / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D008055:Lipids; D011506:Proteins; D014815:Vitamins; D014810:Vitamin E; D008315:Malondialdehyde; D004247:DNA; D011392:Proline; D050842:1-Pyrroline-5-Carboxylate Dehydrogenase; D011394:Proline Oxidase; D001205:Ascorbic Acid
keywords: DNA; Hyperprolinemia; Lipid peroxidation; Protein; Vitamins E and C.