title: Multitarget real-time PCR-based system: monitoring for unauthorized genetically modified events in India.
abstract: A multitarget TaqMan real-time PCR (RTi-PCR) based system was developed to monitor unauthorized genetically modified (GM) events in India. Most of the GM events included in this study are either authorized for commercial cultivation or field trials, which were indigenously developed or imported for research purposes. The developed system consists of a 96-well prespotted plate with lyophilized primers and probes, for simultaneous detection of 47 targets in duplicate, including 21 event-specific sequences, 5 construct regions, 15 for transgenic elements, and 6 taxon-specific targets for cotton, eggplant, maize, potato, rice, and soybean. Limit of detection (LOD) of assays ranged from 0.1 to 0.01% GM content for different targets. Applicability, robustness, and practical utility of the developed system were verified with stacked GM cotton event, powdered samples of proficiency testing and two unknown test samples. This user-friendly multitarget approach can be efficiently utilized for monitoring the unauthorized GM events in an Indian context.
authors: Division of Genomic Resources, National Bureau of Plant Genetic Resources, New Delhi 110 012, India.
authors: Gurinder Jit
authors: GJ
authors: Randhawa
authors: Monika
authors: M
authors: Singh
authors: Payal
authors: P
authors: Sood
authors: Rajesh K
authors: RK
authors: Bhoge
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D007194:India; D057230:Limit of Detection; D030821:Plants, Genetically Modified / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers