title: In vitro effects of selected contact lens care solutions on Acanthamoeba castellanii strains in Poland.
abstract: Free-living, cosmopolitan amoebae of the Acanthamoeba genus may be the causative agents of Acanthamoeba keratitis (AK) - a progressive, vision-threatening infection of the human cornea described particularly among contact lens wearers. Use of contact lens care solutions, effective against these organisms, is important in preventing AK infections. 3 different strains of Acanthamoeba castellanii of the T4 genotype (Neff strain and two others, isolated from patients with AK) were exposed to 4 selected multipurpose contact lens care solutions available in Poland: Ciba Vision AoSept Plus, Bausch & Lomb ReNu MultiPlus, Alcon Opti-Free, Ciba Vision Solo Care Aqua. No amoebicidal effect was observed. The strongest amoebostatic effect was visible after 24h of exposition to Opti-Free and ReNu solution and associated with percentage increase of rounded, motionless forms. This is significantly longer than minimum disinfection time recommended by manufacturers of all tested multipurpose solutions. Surprisingly, no clear induction of the encystation process was observed.
authors: Department of Medical Biology, Medical University of Warsaw, 73 Nowogrodzka Str., 02-018 Warsaw, Poland. Electronic address: mpadzik@wum.edu.pl.
authors: Marcin
authors: M
authors: Padzik
authors: Department of Medical Biology, Medical University of Warsaw, 73 Nowogrodzka Str., 02-018 Warsaw, Poland.
authors: Lidia
authors: L
authors: Chomicz
authors: Department of Ophthalmology, SPKSO Ophthalmic Hospital, Medical University of Warsaw, 13 Sierakowskiego Str., 03-709 Warsaw, Poland.
authors: Jacek P
authors: JP
authors: Szaflik
authors: Department of Medical Biology, Medical University of Warsaw, 73 Nowogrodzka Str., 02-018 Warsaw, Poland.
authors: Agnieszka
authors: A
authors: Chruścikowska
authors: Department of Orthodontics, Medical University of Warsaw, 59 Nowogrodzka Str., 02-005 Warsaw, Poland.
authors: Konrad
authors: K
authors: Perkowski
authors: Department of Ophthalmology, SPKSO Ophthalmic Hospital, Medical University of Warsaw, 13 Sierakowskiego Str., 03-709 Warsaw, Poland.
authors: Jerzy
authors: J
authors: Szaflik
mesh_terms: D015823:Acanthamoeba Keratitis / Q000469:parasitology / Q000517:prevention & control*; D048248:Acanthamoeba castellanii / Q000187:drug effects* / Q000254:growth & development; D018639:Contact Lens Solutions / Q000494:pharmacology*; D006801:Humans; D008858:Microscopy, Phase-Contrast; D033661:Oocysts / Q000187:drug effects; D011044:Poland; D013997:Time Factors; D053058:Trophozoites / Q000187:drug effects
chemical_list: D018639:Contact Lens Solutions
keywords: Acanthamoeba strains; Hydrogen peroxide; Multipurpose contact lens care solution