title: Comparative study between univariate spectrophotometry and multivariate calibration as analytical tools for simultaneous quantitation of Moexipril and Hydrochlorothiazide.
abstract: Three simple, accurate, reproducible, and selective methods have been developed and subsequently validated for the simultaneous determination of Moexipril (MOX) and Hydrochlorothiazide (HCTZ) in pharmaceutical dosage form. The first method is the new extended ratio subtraction method (EXRSM) coupled to ratio subtraction method (RSM) for determination of both drugs in commercial dosage form. The second and third methods are multivariate calibration which include Principal Component Regression (PCR) and Partial Least Squares (PLSs). A detailed validation of the methods was performed following the ICH guidelines and the standard curves were found to be linear in the range of 10-60 and 2-30 for MOX and HCTZ in EXRSM method, respectively, with well accepted mean correlation coefficient for each analyte. The intra-day and inter-day precision and accuracy results were well within the acceptable limits.
authors: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
authors: Shereen M
authors: SM
authors: Tawakkol
authors: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
authors: M
authors: M
authors: Farouk
authors: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
authors: Omar Abd
authors: OA
authors: Elaziz
authors: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6th of October, Egypt. Electronic address: hemmdan@yahoo.com.
authors: A
authors: A
authors: Hemdan
authors: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors: Mostafa A
authors: MA
authors: Shehata
mesh_terms: D000959:Antihypertensive Agents / Q000032:analysis*; D002138:Calibration; D006852:Hydrochlorothiazide / Q000032:analysis*; D016018:Least-Squares Analysis; D057230:Limit of Detection; D013053:Spectrophotometry / Q000379:methods; D013607:Tablets; D044005:Tetrahydroisoquinolines / Q000032:analysis*
chemical_list: D000959:Antihypertensive Agents; D013607:Tablets; D044005:Tetrahydroisoquinolines; D006852:Hydrochlorothiazide; C058302:moexipril
keywords: Extended ratio subtraction method; Hydrochlorothiazide; Moexipril; PCR; PLSs