title: Implications of DNA leakage in eyes of mutant mice.
abstract: Extranuclear DNA (enDNA) is not well studied ultrastructurally in the retinal pigment epithelium (RPE). We analyzed the retina and vastus medialis muscle of four mouse strains that are related to focal retinal degeneration by transmission electron microscopy (TEM) and EM immunolabeling. Evaluation of enDNA would imply the involvements of enDNA is either limited to the affected tissue or generalized in the whole body. Ultrastructural analysis and EM immunolabeling revealed that enDNA was present in the RPE cells but not in the muscle. These data suggest that enDNA could be unique to unhealthy RPE and a potential biomarker for cellular abnormality.
authors: Laboratory of Immunology, National Eye Institute , Bethesda, MA , USA and.
authors: Alexander J
authors: AJ
authors: Ogilvy
authors: Defen
authors: D
authors: Shen
authors: Yujuan
authors: Y
authors: Wang
authors: Chi-Chao
authors: CC
authors: Chan
authors: Mones S
authors: MS
authors: Abu-Asab
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000378:metabolism / Q000648:ultrastructure; D009154:Mutation / Q000235:genetics; D012160:Retina / Q000378:metabolism* / Q000648:ultrastructure*; D055213:Retinal Pigment Epithelium / Q000378:metabolism* / Q000648:ultrastructure*
chemical_list: D004247:DNA
keywords: Autophagy; RPE; enDNA; extranuclear DNA; hypoxia; mitochondria; retinal pigment epithelium; vastus medialis