title: Platelet vacuoles in a dog with severe nonregenerative anemia: evidence of platelet autophagy.
abstract: A 13-year-old neutered male English Springer Spaniel was presented to The Ohio State University Veterinary Medical Center for evaluation of severe anemia. Upon blood smear review, approximately 50% of the platelets contained single to multiple variably sized clear vacuoles. Transmission electron microscopy of the platelets revealed hallmark features of autophagy, including membrane-lined vesicles and vacuoles containing membrane whorls and degrading organelles. While autophagy has been demonstrated in a wide range of eukaryotic cells for decades, reports of platelet autophagy are lacking. This case report illustrates atypical platelet vacuolation with electron microscopic features characteristic of autophagy.
authors: Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.
authors: Emily M
authors: EM
authors: Pieczarka
authors: Mamoru
authors: M
authors: Yamaguchi
authors: Maxey L
authors: ML
authors: Wellman
authors: M
authors: M
authors: Judith Radin
mesh_terms: D000740:Anemia / Q000097:blood / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D001343:Autophagy; D001792:Blood Platelets / Q000473:pathology / Q000648:ultrastructure*; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000097:blood / Q000473:pathology*; D004285:Dogs; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D014617:Vacuoles / Q000648:ultrastructure*
keywords: Autophagosome; canine; transmission electron microscopy; vacuolization