title: Access to cerebrospinal fluid in piglets via the cisterna magna: optimization and description of the technique.
abstract: The collection of cerebrospinal fluid is necessary in order to determine its composition. It can then be used to diagnose various diseases. The aim of the study was to develop and optimize a technique for performing safe centesis for the collection of cerebrospinal fluid in piglets and its injection through the cisterna magna. The study was divided into three phases: (1) anatomical study of cadavers, (2) in vivo application of the technique and (3) observation of recovery time. The proposed technique resulted in a safe puncture of the cisterna magna. The authors identified and confirmed the correspondence of the crista occipitalis and the wings of the atlas with the external landmarks on the cadaver by means of direct radiological visualization. The punctures were performed successfully at the first attempt in 11 out of 12 anaesthetized piglets. The technique herein described provides a reproducible safe and easy route for approaching the cisterna magna for cerebrospinal fluid collection, drug administration and gene delivery.
authors: Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy noemi.romagnoli@unibo.it.
authors: Noemi
authors: N
authors: Romagnoli
authors: Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
authors: Domenico
authors: D
authors: Ventrella
authors: Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
authors: Massimo
authors: M
authors: Giunti
authors: Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
authors: Francesco
authors: F
authors: Dondi
authors: Telethon Institute of Genetics and Medicine, Napoli, Italy.
authors: Nicolina C
authors: NC
authors: Sorrentino
authors: Telethon Institute of Genetics and Medicine, Napoli, Italy.
authors: Alessandro
authors: A
authors: Fraldi
authors: Telethon Institute of Genetics and Medicine, Napoli, Italy.
authors: Enrico M
authors: EM
authors: Surace
authors: Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
authors: Maria L
authors: ML
authors: Bacci
mesh_terms: D000818:Animals; D002555:Cerebrospinal Fluid; D002946:Cisterna Magna; D005260:Female; D008297:Male; D011677:Punctures; D034421:Sus scrofa; D014696:Ventriculostomy / Q000295:instrumentation / Q000662:veterinary*
keywords: centesis technique; cerebrospinal fluid; cisterna magna; general anaesthesia; piglets