title: Expression of transcribed ultraconserved regions of genome in rat cerebral cortex.
abstract: Emerging evidence indicates that 481 regions of the genome (>200 bp) that actively transcribe noncoding RNAs shows 100% homology between humans, rats and mice. These transcribed ultraconserved regions (T-UCRs) are thought to control the essential regulatory functions basic for life in rodents and mammals. Using microarray analysis, we presently show that 107 T-UCRs are actively expressed in adult rat cerebral cortex. They are grouped into intragenic (61) and intergenic (46) based on their genic location. Interestingly, 10 T-UCRs are expressed at unusually high levels in cerebral cortex. Additionally, many T-UCRs also showed cogenic expression. We further analyzed the correlation of intragenic T-UCRs with their host protein coding genes. Surprisingly, most of the expressed intragenic T-UCRs (54 out of 61) displayed a negative correlation with their host gene expression. T-UCRs are thought to control the splicing and transcription of the protein-coding genes that host them and flank them. Bioinformatics analysis indicated that the protein products of majority of these genes are nuclear in localization, share protein domains and are involved in the regulation of diverse biological and molecular functions including metabolism, development, cell cycle, binding and transcription factor regulation. In conclusion, this is the first study to shows that many T-UCRs are expressed in rodent brain and they might play a role in physiological brain functions.
authors: Department of Neurological Surgery, University of Wisconsin, Madison, WI, USA.
authors: Suresh L
authors: SL
authors: Mehta
authors: Department of Neurological Surgery, University of Wisconsin, Madison, WI, USA; Theoretical Biology and Biophysics (T-6), Los Alamos National Laboratory, Los Alamos, NM, USA.
authors: Ashutosh
authors: A
authors: Dharap
authors: Department of Neurological Surgery, University of Wisconsin, Madison, WI, USA. Electronic address: Vemuganti@neurosurgery.wisc.edu.
authors: Raghu
authors: R
authors: Vemuganti
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000235:genetics; D002540:Cerebral Cortex / Q000378:metabolism*; D019295:Computational Biology; D017124:Conserved Sequence / Q000235:genetics*; D053263:Gene Regulatory Networks / Q000235:genetics; D016678:Genome / Q000235:genetics*; D058977:Molecular Sequence Annotation; D051381:Rats
keywords: Brain; Gene expression; Non-coding RNA; Transcription; lncRNA