title: Changes in metabolome and in enzyme activities during germination of Trichoderma atroviride conidia.
abstract: The aim of this work was to study the metabolic changes during germination of Trichoderma atroviride conidia along with selected marker enzyme activities. The increase in proteinogenic amino acid concentrations together with the increase in glutamate dehydrogenase activity suggests a requirement for nitrogen metabolism. Even though the activities of tricarboxylic acid cycle enzymes also increased, detected organic acid pools did not change, which predisposes this pathway to energy production and supply of intermediates for further metabolism. The concentrations of many metabolites, including the main osmolytes mannitol and betaine, also increased during the formation of germ tubes. The activities of H(+)-ATPase and GDPase, the only marker enzymes that did not have detectable activity in non-germinated conidia, were shown with germ tubes.
authors: Department of Biochemistry and Microbiology, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovakia.
authors: Michal
authors: M
authors: Kaliňák
authors: Martin
authors: M
authors: Simkovič
authors: Peter
authors: P
authors: Zemla
authors: Matej
authors: M
authors: Matata
authors: Tomáš
authors: T
authors: Molnár
authors: Tibor
authors: T
authors: Liptaj
authors: L'udovít
authors: L
authors: Varečka
authors: Daniela
authors: D
authors: Hudecová
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001622:Betaine / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis; D004798:Enzymes / Q000378:metabolism*; D008353:Mannitol / Q000032:analysis; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D009584:Nitrogen / Q000378:metabolism; D013172:Spores, Fungal / Q000737:chemistry* / Q000201:enzymology / Q000254:growth & development*; D014242:Trichoderma / Q000737:chemistry* / Q000201:enzymology / Q000254:growth & development*
chemical_list: D000596:Amino Acids; D002264:Carboxylic Acids; D004798:Enzymes; D008353:Mannitol; D001622:Betaine; D009584:Nitrogen
keywords: Trichoderma atroviride; conidia; germination; marker enzymes; metabolomics