title: Evaluation of NCS-1, DARPP-32, and neurotrophins in hippocampus and prefrontal cortex in rats submitted to sepsis.
abstract: Sepsis is defined as the host's reaction to infection and it is characterized by a systemic inflammatory response with important clinical implications. Central nervous system dysfunction secondary to sepsis is associated with local generation of pro- and anti-inflammatory cytokines, impaired cerebral microcirculation, disturbance of neurotransmitters, apoptosis, and cognitive impairment. It is known that during the process of learning and memory formation several pathways are involved such as dopaminergic and cholinergic systems. Thus, the objective of this study is to evaluate the neuronal calcium sensor (NCS-1) and dopamine-cAMP regulated phosphoprotein of 32,000 kDa (DARPP-32) expression as well as brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) levels in prefrontal cortex and hippocampus of rats 12, 24, and 48 h after sepsis induction. To this aim, we used sham-operated Wistar rats or submitted to the cecal ligation and perforation procedure. After 12 and 24 h, there was an increase of NGF levels in hippocampus; and up to 48 h, a decrease of NCS-1 expression in prefrontal cortex, a decrease of BDNF levels in hippocampus and an increase of NGF levels in hippocampus. In conclusion, we believe that the low expression of NCS-1 in prefrontal cortex and low levels of BDNF in hippocampus may be associated with the pathophysiology of cognitive impairment during sepsis and a putative role of the dopaminergic system.
authors: Postgraduate Program in Health Sciences, Laboratory of Experimental Neurosciences, Infectious Diseases Unit, University of South Santa Catarina, Palhoça, Santa Catarina, Brazil.
authors: Clarissa M
authors: CM
authors: Comim
authors: Napoleão C
authors: NC
authors: Silva
authors: Francielle
authors: F
authors: Mina
authors: Diogo
authors: D
authors: Dominguini
authors: Giselli
authors: G
authors: Scaini
authors: Meline O S
authors: MO
authors: Morais
authors: Daniela V
authors: DV
authors: Rosa
authors: Luiz Alexandre V
authors: LA
authors: Magno
authors: Emilio L
authors: EL
authors: Streck
authors: Marco A
authors: MA
authors: Romano-Silva
authors: João
authors: J
authors: Quevedo
authors: Felipe
authors: F
authors: Dal-Pizzol
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D004195:Disease Models, Animal; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006624:Hippocampus / Q000378:metabolism*; D015151:Immunoblotting; D008297:Male; D020932:Nerve Growth Factor / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism*; D051580:Neuronal Calcium-Sensor Proteins / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism*; D017208:Rats, Wistar; D018805:Sepsis / Q000378:metabolism*; D013997:Time Factors
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009414:Nerve Growth Factors; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; C497930:Ppp1r1b protein, rat; C101464:frequenin calcium sensor proteins; D020932:Nerve Growth Factor
keywords: BDNF; NGF; brain; cognitive impairment