title: Radiofrequency pancreatic ablation and section of the main pancreatic duct does not lead to necrotizing pancreatitis.
abstract: OBJECTIVE
  The aim of this study was to determine whether radiofrequency ablation (RFA) of the pancreas and subsequent transection of the main pancreatic duct may avoid the risk of both necrotizing pancreatitis and postoperative pancreatic fistula (POPF) formation.
  
  
  METHODS
  Thirty-two rats were subjected to RFA and section of the pancreas over their portal vein. Animals were killed at 3, 7, 15, and 21 days (groups 0-3, respectively). Two additional control groups (sham operation and user manipulation only, respectively) of 15 days of postoperative period were considered. Postoperative complications, histological changes (including morphometric and immunohistochemical analysis), and incidence of POPF were evaluated.
  
  
  RESULTS
  A significant increase in serum amylase levels (P < 0.05) on the third postoperative day, which return to baseline levels in the following weeks, was noted in groups 0 to 3. Those groups showed a rapid atrophy of the distal pancreas by apoptosis with no signs of necrotizing pancreatitis or POPF. The distal pancreas in groups 1 to 3 compared with group 0 and control groups showed a significant increase of small islets (<1000 µm).
  
  
  CONCLUSIONS
  The rapid acinar atrophy of the distal pancreas after RFA and section of the pancreatic ducts in this model does not lead to necrotizing pancreatitis.
authors: From the *Instituto Municipal de Investigación Médica; Departments of †Surgery and ‡Pathology, Hospital del Mar; and §Department of Animal Surgery, Veterinary Faculty, University of Barcelona, Barcelona; ∥Department of Pathology, Hospital Clínico Universitario "Lozano Blesa," Zaragoza; and ¶Department of Electronic Engineering, Polytechnic University of Valencia, Valencia, Spain.
authors: Rita
authors: R
authors: Quesada
authors: Fernando
authors: F
authors: Burdío
authors: Mar
authors: M
authors: Iglesias
authors: Dimitri
authors: D
authors: Dorcaratto
authors: Marta
authors: M
authors: Cáceres
authors: Anna
authors: A
authors: Andaluz
authors: Ignasi
authors: I
authors: Poves
authors: Tomás
authors: T
authors: Castiella
authors: Patricia
authors: P
authors: Sánchez
authors: Enrique
authors: E
authors: Berjano
authors: Luis
authors: L
authors: Grande
mesh_terms: D000818:Animals; D017115:Catheter Ablation / Q000009:adverse effects / Q000379:methods*; D005260:Female; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D010183:Pancreatic Ducts / Q000601:surgery*; D010185:Pancreatic Fistula / Q000175:diagnosis / Q000209:etiology; D019283:Pancreatitis, Acute Necrotizing / Q000175:diagnosis* / Q000209:etiology; D011169:Portal Vein / Q000601:surgery; D011183:Postoperative Complications / Q000175:diagnosis* / Q000209:etiology; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D016896:Treatment Outcome
chemical_list: D007328:Insulin