title: Infection of Beet necrotic yellow vein virus with RNA4-encoded P31 specifically up-regulates pathogenesis-related protein 10 in Nicotiana benthamiana.
abstract: BACKGROUND
  Beet necrotic yellow vein virus (BNYVV) is the infectious agent of sugar beet rhizomania, which consists of four or five plus-sense RNAs. RNA4 of BNYVV is not essential for virus propagation in Nicotiana benthamiana but has a major effect on symptom expression. Early reports showed that RNA4-encoded P31 was associated with severe symptoms, such as curling and dwarfing, in N. benthamiana.
  
  
  RESULTS
  We discovered that the pathogenesis-related protein 10 (PR-10) gene can be up-regulated in BNYVV-infected N. benthamiana in the presence of RNA4 and that it had a close link with symptom development. Our frame-shift, deletion and substitution analysis showed that only the entire P31 could induce PR-10 up-regulation during BNYVV infection and that all the tryptophans and six cysteines (C174, C183, C186, C190, C197 and C199) in the cysteine-rich P31 had significant effects on PR-10 expression. However, P31 could not interact directly with PR-10 in yeast.
  
  
  CONCLUSIONS
  Our data demonstrated that only integrated P31 specifically induced PR-10 transcription, which coincided closely with the appearance of severe symptoms in BNYVV-infected N. benthamiana, although they could not interact directly with each other in yeast.
authors: Wen-Qi
authors: WQ
authors: Wu
authors: Hui-Yan
authors: HY
authors: Fan
authors: Ning
authors: N
authors: Jiang
authors: Ying
authors: Y
authors: Wang
authors: Zong-Ying
authors: ZY
authors: Zhang
authors: Yong-Liang
authors: YL
authors: Zhang
authors: Xian-Bing
authors: XB
authors: Wang
authors: Da-Wei
authors: DW
authors: Li
authors: Jia-Lin
authors: JL
authors: Yu
authors: State Key Laboratory of Agrobiotechnology and the Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China. hanchenggui@cau.edu.cn.
authors: Cheng-Gui
authors: CG
authors: Han
mesh_terms: D004252:DNA Mutational Analysis; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D010940:Plant Proteins / Q000096:biosynthesis*; D012328:RNA Viruses / Q000502:physiology*; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000821:virology*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014764:Viral Proteins; C053376:pathogenesis-related proteins, plant