title: Insulin receptor expression in clear cell renal cell carcinoma and its relation to prognosis.
abstract: OBJECTIVE
  Both insulin and insulin-like growth factor (IGF)-1 signaling are key regulators of energy metabolism, cellular growth, proliferation, and survival. The IGF-1 receptor (IGF-1R) is overexpressed in most types of human cancers including renal cell carcinoma (RCC) with poor prognosis. Insulin receptor (IR) shares downstream effectors with IGF-1R; however, the expression and function of IR in the tumorigenesis of renal cancer remains elusive. Therefore, we examined the expression of IR and its prognostic significance in clear cell RCC (CCRCC).
  
  
  METHODS
  Immunohistochemical staining for IR was performed on 126 formalin-fixed paraffin-embedded CCRCC tissue samples. Eight of these cases were utilized for western blot analysis. The results were compared with various clinico-pathologic parameters of CCRCC and patient survival.
  
  
  RESULTS
  IR was expressed in the nuclei of CCRCC tumor cells in 109 cases (87.9%). Higher IR expression was significantly correlated with the presence of cystic change, lower Fuhrman nuclear grade, lower pathologic T stage, and lower TNM stage, although it wasn't significantly related to diabetes status and patient survival. Western blot analyses supported the results of the immunohistochemistry studies.
  
  
  CONCLUSIONS
  IR expression in CCRCC may be associated with favorable prognostic factors.
authors: Department of Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Sayamaa
authors: S
authors: Lkhagvadorj
authors: Department of Occupational & Environmental Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Sung Soo
authors: SS
authors: Oh
authors: Department of Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Mi-Ra
authors: MR
authors: Lee
authors: Department of Urology, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Jae Hung
authors: JH
authors: Jung
authors: Department of Urology, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Hyun Chul
authors: HC
authors: Chung
authors: Department of Physiology, Yonsei University Wonju College of Medicine, Wonju, Korea. ; Institute of Lifestyle Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors: Seung-Kuy
authors: SK
authors: Cha
authors: Department of Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea. eomm@yonsei.ac.kr.
authors: Minseob
authors: M
authors: Eom
mesh_terms: D000368:Aged; D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011972:Receptor, Insulin / Q000378:metabolism*
chemical_list: D011972:Receptor, Insulin
keywords: Insulin receptor; clear cell renal carcinoma; diabetes mellitus; immunohistochemistry; prognosis