title: Involvement of TSP1 and MMP9/NGAL in angiogenesis during orthodontic periodontal remodeling.
abstract: In the present study the aim was to measure the levels of Thrombospondin-1 (TSP1) and Lipocalin-2/matrix metalloproteinase 9 (MMP9/NGAL) complex in gingival crevicular fluid (GCF) at different time points of orthodontic treatment, to determine the relationship between these values and those of total-matrix metalloproteinase 9 (MMP9) and theirs implication in angiogenesis balance, in the situation of a good control of the bacterial plaque, emphasizing the role of TSP1 and MMP9/NGAL complex. GCF samples were collected from 16 young orthodontic patients requiring upper canine distalization (test tooth) with first premolar extraction. The contralateral canine (control tooth) was free from orthodontic force. For the orthodontic appliance, brackets Roth 0.018 inch with 0.012 inch NiTi archwire and a laceback were used. TSP1, MMP9/NGAL, and MMP9 increased from 1 hour before activation of orthodontic appliance to a maximum at 8 hours for MMP9 and 72 hours for MMP9/NGAL and TSP1. The results show a change in time of TSP1, MMP9/NGAL, and MMP9 levels in GCF of patients with this method of orthodontic treatment. The powerful correlation of MMP9/NGAL with TSP1 suggests their stronger involvement in angiogenesis processes in PDL during orthodontic periodontal remodeling, in the situation of a healthy periodontium and a good control of the bacterial plaque.
authors: Department of Periodontology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Petra
authors: P
authors: Surlin
authors: Department of Immunology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Isabela
authors: I
authors: Silosi
authors: Department of Orthodontics, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Anne Marie
authors: AM
authors: Rauten
authors: Department of Physiology, University of Medicine Titu Maiorescu of Bucharest, 187 Calea Vacaresti Street, 040051 Bucharest, Romania.
authors: Manole
authors: M
authors: Cojocaru
authors: Department of Biochemistry, University of Medicine and Pharmacy "Gr. T. Popa" Iasi, 16 Universitatii Street, 700115 Iasi, Romania.
authors: Lili
authors: L
authors: Foia
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism*; D000293:Adolescent; D005260:Female; D005883:Gingival Crevicular Fluid / Q000378:metabolism; D006801:Humans; D000071068:Lipocalin-2; D054834:Lipocalins / Q000378:metabolism*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010519:Periodontium / Q000098:blood supply* / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019700:Thrombospondin 1 / Q000378:metabolism*
chemical_list: D000209:Acute-Phase Proteins; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D011518:Proto-Oncogene Proteins; D019700:Thrombospondin 1; D020780:Matrix Metalloproteinase 9