title: Exploring the importance of translational regulation in the inflammatory responses by a genome-wide approach.
abstract: It is widely recognized that exercise has an important role in inflammation regulation. To understand inflammatory mechanisms, extensive studies on the transcriptome and proteome have been conducted. However, interpreting these results is difficult, partly due to technical difficulties that impose some restriction on the accuracy and comprehensiveness of measurements. Here we first mention some limitations of studies involving large scale proteomics and high-throughput transcriptomics and further introduce a newly developed genome-wide translational analysis which may overcome some of the limitations and discover novel cellular dynamics. We then show preliminary results obtained by conducting a genome-wide translational analysis of the early inflammatory response of macrophages in response to lipopolysaccharide (LPS), and discuss the potential to identify novel factors by employing a genome-wide translational analysis.
authors: Hiroaki
authors: H
authors: Sako
authors: Katsuhiko
authors: K
authors: Suzuki
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D055106:Genome-Wide Association Study / Q000379:methods*; D007249:Inflammation / Q000235:genetics* / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000235:genetics*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames / Q000235:genetics; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D015203:Reproducibility of Results; D012270:Ribosomes / Q000378:metabolism; D017423:Sequence Analysis, RNA
chemical_list: D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D011506:Proteins; D012333:RNA, Messenger; C482199:lipopolysaccharide, E coli O55-B5; C031017:ornithine decarboxylase antizyme