title: Glycocaulis albus sp. nov., a moderately halophilic dimorphic prosthecate bacterium isolated from petroleum-contaminated saline soil.
abstract: Two novel bacterial strains, SLG210-30A1(T) and SLG210-19A2, which shared 99.9 % 16S rRNA gene sequence similarity with each other, were isolated from petroleum-contaminated saline soil in Shengli Oilfield, eastern China. Cells were Gram-stain-negative, motile, aerobic, mesophilic and moderately halophilic. They could grow chemoheterotrophically with oxygen as an electron acceptor. Morphologically, cells were typical Caulobacteria-type dimorphic prosthecate bacteria. The genomic DNA G+C contents of strains SLG210-30A1(T) and SLG210-19A2 were 61.8 mol% and 61.6 mol% respectively. Strain SLG210-30A1(T) had Q10 as the predominant respiratory ubiquinone, and C16 : 0 (28.4 %), C17 : 0 (11.6 %), C18 : 0 (22.1 %) and C18 : 1ω7c (14.0 %) as the major cellular fatty acids. The polar lipids of the two isolates were some glycolipids, a lipid, a phospholipid, an aminoglycolipid and an aminophospholipid (all unidentified). The 16S rRNA gene sequences of strains SLG210-30A1(T) and SLG210-19A2 showed the highest similarities with Glycocaulis abyssi MCS 33(T) (99.8-99.9 %), but low sequence similarities (<94.7 %) with type strains of other members of the family Hyphomonadaceae. However, the DNA-DNA relatedness of G. abyssi MCS 33(T) to strains SLG210-30A1(T) and SLG210-19A2 was 37.4±4.4 % and 36.1±1.1 %, respectively. Based on different physiological, biochemical, and phylogenetic characteristics, strains SLG210-30A1(T) and SLG210-19A2 represent a novel species of the genus Glycocaulis. The name Glycocaulis albus is therefore proposed with strain SLG210-30A1(T) ( = LMG 27741(T) = CGMCC 1.12766(T)) as the type strain. An emended description of the genus Glycocaulis is also provided.
authors: College of Engineering, Peking University, Beijing 100871, PR China.
authors: Xiang-Lin
authors: XL
authors: Lv
authors: College of Engineering, Peking University, Beijing 100871, PR China.
authors: Bai-Sheng
authors: BS
authors: Xie
authors: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Man
authors: M
authors: Cai
authors: College of Engineering, Peking University, Beijing 100871, PR China.
authors: Shuang
authors: S
authors: Geng
authors: College of Architecture and Environment, Sichuan University, Chengdu, 610065, PR China.
authors: Yue-Qin
authors: YQ
authors: Tang
authors: Institute of Biology, Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors: Ya-Nan
authors: YN
authors: Wang
authors: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors: Heng-Lin
authors: HL
authors: Cui
authors: College of Engineering, Peking University, Beijing 100871, PR China.
authors: Xue-Ying
authors: XY
authors: Liu
authors: Qingdao Institute of Marine Geology, No. 62, Fuzhou Rd. Qingdao 266071, PR China.
authors: Si-Yuan
authors: SY
authors: Ye
authors: College of Engineering, Peking University, Beijing 100871, PR China.
authors: Xiao-Lei
authors: XL
authors: Wu
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004787:Environmental Pollution; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010578:Petroleum; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010578:Petroleum; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2