title: Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus.
abstract: OBJECTIVE
  We have designed ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles associated with fucoidan (USPOI-FUCO), a natural sulfated polysaccharide with high affinity for activated platelets, to visualize by MRI arterial thrombi.
  
  
  METHODS
  USPIOs were prepared and sizes, zeta-potentials and relaxivities were measured. Elastase perfusion in the infrarenal aorta of Wistar rats induced intraluminal thrombus. They were scanned on 4.7 T MRI before and after injection of USPIO-FUCO or USPIO coated with anionic dextran.
  
  
  RESULTS
  Surface plasmon resonance evidenced that fucoidan and USPIO-FUCO bind in vitro to immobilized P-selectin. All intraluminal hyposignals detected by MRI after injection of USPIO-FUCO on animals (13 out of 13) were correlated by histology with thrombi, whereas none could be identified with control USPIOs (0 out of 7). No signal was seen in absence of thrombus. Thrombi by MRI were correlated with P-selectin immunostaining and USPIO detection by electron microscopy.
  
  
  CONCLUSIONS
  In vivo thrombi can thus be evidenced by MRI with USPIO-FUCO.
authors: French Institute of Health & Medical Research (Inserm) U1148, Laboratory for Vascular Translational Science, CHU X Bichat, University Paris 7, 46 rue H Huchard, F-75877, France.
authors: Michimasa
authors: M
authors: Suzuki
authors: Laure
authors: L
authors: Bachelet-Violette
authors: François
authors: F
authors: Rouzet
authors: Anne
authors: A
authors: Beilvert
authors: Gwennhael
authors: G
authors: Autret
authors: Murielle
authors: M
authors: Maire
authors: Christine
authors: C
authors: Menager
authors: Liliane
authors: L
authors: Louedec
authors: Christine
authors: C
authors: Choqueux
authors: Pierre
authors: P
authors: Saboural
authors: Oualid
authors: O
authors: Haddad
authors: Cédric
authors: C
authors: Chauvierre
authors: Frédéric
authors: F
authors: Chaubet
authors: Jean-Baptiste
authors: JB
authors: Michel
authors: Jean-Michel
authors: JM
authors: Serfaty
authors: Didier
authors: D
authors: Letourneur
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage; D005290:Ferric Compounds / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D018810:Magnetic Resonance Angiography; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D013927:Thrombosis / Q000000981:diagnostic imaging* / Q000473:pathology
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D011134:Polysaccharides; C000499:ferric oxide; C007789:fucoidan
keywords: MRI; P-selectin; aneurysm; iron oxide nanoparticle; thrombus