title: In vitro antimicrobial effects of 3 root canal sealers on Actinomyces radicidentis.
abstract: OBJECTIVE
  To determine the in vitro antimicrobial effects of 3 endodontic sealers--AH Plus, Sealapex, and Tubli-Seal--on Actinomyces radicidentis, a bacterial species commonly found in root canals.
  
  
  METHODS
  Prior to the experimental procedures, bacterial identification tests, such as Gram staining, catalase, and API 20A, were performed, and the bacteria were identified as A. radicidentis. The agar diffusion susceptibility test was performed to determine the areas of bacterial growth inhibition and, consequently, the microbial resistance of the 3 sealers against A. radicidentis. Chlorhexidine was used as a positive control, and saline solution was used as a negative control.
  
  
  RESULTS
  Tubli-Seal cement had an average diameter of inhibition zones in the 3 panels of 22.73 mm, that of AH Plus was 17.13 mm, and that of Sealapex, 11.99 mm. A one-way ANOVA test showed that there were significant differences between the 3 cements (p < 0.05).
  
  
  CONCLUSIONS
  Tubli-Seal showed the highest levels of antimicrobial activity, which was followed by AH Plus with the next highest levels and, finally, Sealapex with the lowest levels of antimicrobial activity.
authors: Ignacio
authors: I
authors: Barbero-Navarro
authors: Hugo
authors: H
authors: Galera-Ruiz
authors: Mario
authors: M
authors: Pereira
authors: Diogo
authors: D
authors: Guerreiro
authors: María del Carmen
authors: Mdel C
authors: Machuca-Portillo
authors: Lydia
authors: L
authors: López del Valle
mesh_terms: D000190:Actinomyces / Q000187:drug effects*; D000704:Analysis of Variance; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000494:pharmacology; D002710:Chlorhexidine / Q000494:pharmacology; D003786:Dental Pulp Cavity / Q000382:microbiology; D004853:Epoxy Resins / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012459:Salicylates / Q000494:pharmacology; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D000891:Anti-Infective Agents, Local; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012459:Salicylates; D015035:Zinc Oxide-Eugenol Cement; C534916:epoxy resin-based root canal sealer; C055601:Sealapex; C048769:tubliseal; D002126:Calcium Hydroxide; D002710:Chlorhexidine