title: Isolation and characterisation of methanol-soluble fraction of Alternanthera philoxeroides (Mart.) - evaluation of their antioxidant, α-glucosidase inhibitory and antimicrobial activity in in vitro systems.
abstract: Alternanthera philoxeroides (Mart.) is a tropical weed commonly known as alligator weed. It grows rapidly within a small span of time and easily available all over the world. The objective of this work was to isolate and characterise the major phenolic components present in the methanol-soluble fraction (fraction X) of A. philoxeroides leaves and to explore the biological activity (antioxidant, α-glucosidase inhibition and antimicrobial) of the fraction in in vitro system. Chromatographic (HPLC) and spectroscopic (MALDI-TOF, ¹H NMR) techniques were used to purify and characterise the phenolics present in fraction X. Five major phenolics (kaempferol, ferulic acid, salicylic acid, syringic acid and chlorogenic acid) were found in fraction X. The fraction showed anti-oxidant property, dose-dependent inhibition of α-glucosidase activity and anti-microbial activity. Hence fraction X from the weed has therapeutic potential in pathophysiological condition.
authors: a Department of Botany , Presidency University , 86/1, College Street, Kolkata 700 073 West Bengal India.
authors: Abhishek
authors: A
authors: Bhattacherjee
authors: Tanushree
authors: T
authors: Ghosh
authors: Rajarshi
authors: R
authors: Sil
authors: Abhijit
authors: A
authors: Datta
mesh_terms: D027703:Amaranthaceae / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002726:Chlorogenic Acid / Q000494:pharmacology; D003373:Coumaric Acids / Q000494:pharmacology; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D066298:In Vitro Techniques; D044949:Kaempferols / Q000494:pharmacology; D000432:Methanol; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D020156:Salicylic Acid / Q000494:pharmacology; D000520:alpha-Glucosidases / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D003373:Coumaric Acids; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D044949:Kaempferols; D010636:Phenols; D002726:Chlorogenic Acid; D005707:Gallic Acid; C006552:kaempferol; C004999:ferulic acid; C001945:syringic acid; D000520:alpha-Glucosidases; D020156:Salicylic Acid; D000432:Methanol
keywords: Alternanthera philoxeroides; antimicrobial; antioxidant; phenolics; α-glucosidase